LOCUS       DAVBCV010000001        83251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBCV010000001 DAVBCV010000000
VERSION     DAVBCV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 83251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.001"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            146..394
                     /locus_tag="VQ516_000001"
     CDS             146..394
                     /locus_tag="VQ516_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510528.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            525..1697
                     /locus_tag="VQ516_000002"
     CDS             525..1697
                     /locus_tag="VQ516_000002"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2510529.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1697..2971
                     /locus_tag="VQ516_000003"
     CDS             1697..2971
                     /locus_tag="VQ516_000003"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2510530.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(3007..3972)
                     /locus_tag="VQ516_000004"
     CDS             complement(3007..3972)
                     /locus_tag="VQ516_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2510531.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQTIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3953..5407)
                     /locus_tag="VQ516_000005"
     CDS             complement(3953..5407)
                     /locus_tag="VQ516_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2510532.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKSQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5784..7667
                     /locus_tag="VQ516_000006"
     CDS             5784..7667
                     /locus_tag="VQ516_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2510533.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            7816..8703
                     /locus_tag="VQ516_000007"
     CDS             7816..8703
                     /locus_tag="VQ516_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2510534.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGTSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(9023..9640)
                     /locus_tag="VQ516_000008"
     CDS             complement(9023..9640)
                     /locus_tag="VQ516_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2510535.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(10055..10243)
                     /locus_tag="VQ516_000009"
                     /pseudo
     CDS             complement(10055..10243)
                     /locus_tag="VQ516_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(10753..10833)
                     /locus_tag="VQ516_000010"
                     /pseudo
     CDS             complement(10753..10833)
                     /locus_tag="VQ516_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10949..11842
                     /locus_tag="VQ516_000011"
     CDS             10949..11842
                     /locus_tag="VQ516_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510536.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11839..13038
                     /locus_tag="VQ516_000012"
     CDS             11839..13038
                     /locus_tag="VQ516_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2510537.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(13391..14158)
                     /locus_tag="VQ516_000013"
     CDS             complement(13391..14158)
                     /locus_tag="VQ516_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510538.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(14175..14870)
                     /locus_tag="VQ516_000014"
     CDS             complement(14175..14870)
                     /locus_tag="VQ516_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510539.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(14875..15240)
                     /locus_tag="VQ516_000015"
     CDS             complement(14875..15240)
                     /locus_tag="VQ516_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2510540.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            15380..15853
                     /gene="nrdR"
                     /locus_tag="VQ516_000016"
     CDS             15380..15853
                     /gene="nrdR"
                     /locus_tag="VQ516_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2510541.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            15854..17023
                     /locus_tag="VQ516_000017"
     CDS             15854..17023
                     /locus_tag="VQ516_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2510542.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            17024..17920
                     /gene="dnaI"
                     /locus_tag="VQ516_000018"
     CDS             17024..17920
                     /gene="dnaI"
                     /locus_tag="VQ516_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2510543.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            17917..18630
                     /locus_tag="VQ516_000019"
     CDS             17917..18630
                     /locus_tag="VQ516_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2510544.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            18644..19954
                     /gene="der"
                     /locus_tag="VQ516_000020"
     CDS             18644..19954
                     /gene="der"
                     /locus_tag="VQ516_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2510545.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            20045..20380
                     /locus_tag="VQ516_000021"
     CDS             20045..20380
                     /locus_tag="VQ516_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510546.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            20520..20855
                     /locus_tag="VQ516_000022"
     CDS             20520..20855
                     /locus_tag="VQ516_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510547.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            21040..21831
                     /locus_tag="VQ516_000023"
     CDS             21040..21831
                     /locus_tag="VQ516_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510548.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            21824..22465
                     /locus_tag="VQ516_000024"
     CDS             21824..22465
                     /locus_tag="VQ516_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510549.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            22469..23119
                     /locus_tag="VQ516_000025"
     CDS             22469..23119
                     /locus_tag="VQ516_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2510550.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            23130..23417
                     /locus_tag="VQ516_000026"
     CDS             23130..23417
                     /locus_tag="VQ516_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510551.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            23563..26076
                     /gene="secA"
                     /locus_tag="VQ516_000027"
     CDS             23563..26076
                     /gene="secA"
                     /locus_tag="VQ516_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2510552.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            26157..27188
                     /locus_tag="VQ516_000028"
     CDS             26157..27188
                     /locus_tag="VQ516_000028"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2510553.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            27190..28221
                     /locus_tag="VQ516_000029"
     CDS             27190..28221
                     /locus_tag="VQ516_000029"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2510554.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            28260..28628
                     /locus_tag="VQ516_000030"
     CDS             28260..28628
                     /locus_tag="VQ516_000030"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2510555.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            28618..29721
                     /locus_tag="VQ516_000031"
     CDS             28618..29721
                     /locus_tag="VQ516_000031"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2510556.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            29740..31755
                     /gene="recG"
                     /locus_tag="VQ516_000032"
     CDS             29740..31755
                     /gene="recG"
                     /locus_tag="VQ516_000032"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2510557.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     IKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            32071..33051
                     /locus_tag="VQ516_000033"
     CDS             32071..33051
                     /locus_tag="VQ516_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2510558.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            33856..34083
                     /locus_tag="VQ516_000034"
     CDS             33856..34083
                     /locus_tag="VQ516_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510559.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            34371..37268
                     /gene="nanA"
                     /locus_tag="VQ516_000035"
     CDS             34371..37268
                     /gene="nanA"
                     /locus_tag="VQ516_000035"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2510560.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGNRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            38283..38735
                     /locus_tag="VQ516_000036"
     CDS             38283..38735
                     /locus_tag="VQ516_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2510561.1"
                     /translation="MIITKISHLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            38754..40091
                     /locus_tag="VQ516_000037"
     CDS             38754..40091
                     /locus_tag="VQ516_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2510562.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            40170..41054
                     /locus_tag="VQ516_000038"
     CDS             40170..41054
                     /locus_tag="VQ516_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2510563.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            41054..41887
                     /locus_tag="VQ516_000039"
     CDS             41054..41887
                     /locus_tag="VQ516_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2510564.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            41905..43998
                     /gene="nanB"
                     /locus_tag="VQ516_000040"
     CDS             41905..43998
                     /gene="nanB"
                     /locus_tag="VQ516_000040"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2510565.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKRSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNREESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            44010..45113
                     /locus_tag="VQ516_000041"
     CDS             44010..45113
                     /locus_tag="VQ516_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2510566.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            45287..45985
                     /locus_tag="VQ516_000042"
     CDS             45287..45985
                     /locus_tag="VQ516_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2510567.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            46004..47521
                     /locus_tag="VQ516_000043"
     CDS             46004..47521
                     /locus_tag="VQ516_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2510568.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFLGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            47646..48974
                     /locus_tag="VQ516_000044"
     CDS             47646..48974
                     /locus_tag="VQ516_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2510569.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            49226..50092
                     /locus_tag="VQ516_000045"
     CDS             49226..50092
                     /locus_tag="VQ516_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2510570.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            50108..50947
                     /locus_tag="VQ516_000046"
     CDS             50108..50947
                     /locus_tag="VQ516_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2510571.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            51220..51672
                     /locus_tag="VQ516_000047"
     CDS             51220..51672
                     /locus_tag="VQ516_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2510572.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            51769..52113
                     /locus_tag="VQ516_000048"
     CDS             51769..52113
                     /locus_tag="VQ516_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510573.1"
                     /translation="MVSSKHAARASFLDGQEITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGIGIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            52397..53041
                     /locus_tag="VQ516_000049"
     CDS             52397..53041
                     /locus_tag="VQ516_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2510574.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            53210..54127
                     /locus_tag="VQ516_000050"
     CDS             53210..54127
                     /locus_tag="VQ516_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2510575.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKESFL"
     gene            54145..55029
                     /locus_tag="VQ516_000051"
     CDS             54145..55029
                     /locus_tag="VQ516_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2510576.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(55045..55896)
                     /locus_tag="VQ516_000052"
     CDS             complement(55045..55896)
                     /locus_tag="VQ516_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2510577.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANRVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            56096..58153
                     /gene="pbp2b"
                     /locus_tag="VQ516_000053"
     CDS             56096..58153
                     /gene="pbp2b"
                     /locus_tag="VQ516_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2510578.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTKRQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            58164..58760
                     /gene="recR"
                     /locus_tag="VQ516_000054"
     CDS             58164..58760
                     /gene="recR"
                     /locus_tag="VQ516_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2510579.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            58936..59979
                     /locus_tag="VQ516_000055"
     CDS             58936..59979
                     /locus_tag="VQ516_000055"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2510580.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            60063..61436
                     /locus_tag="VQ516_000056"
     CDS             60063..61436
                     /locus_tag="VQ516_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2510581.1"
                     /translation="MKLTIHEVAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGVRDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGVQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            61423..62034
                     /locus_tag="VQ516_000057"
     CDS             61423..62034
                     /locus_tag="VQ516_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2510582.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDPLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            62125..62829
                     /locus_tag="VQ516_000058"
     CDS             62125..62829
                     /locus_tag="VQ516_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2510583.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            63048..64421
                     /gene="ftsA"
                     /locus_tag="VQ516_000059"
     CDS             63048..64421
                     /gene="ftsA"
                     /locus_tag="VQ516_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2510584.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENIIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            64438..65697
                     /gene="ftsZ"
                     /locus_tag="VQ516_000060"
     CDS             64438..65697
                     /gene="ftsZ"
                     /locus_tag="VQ516_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2510585.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            65702..66373
                     /locus_tag="VQ516_000061"
     CDS             65702..66373
                     /locus_tag="VQ516_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2510586.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            66383..66922
                     /locus_tag="VQ516_000062"
     CDS             66383..66922
                     /locus_tag="VQ516_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2510587.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            66922..67185
                     /locus_tag="VQ516_000063"
     CDS             66922..67185
                     /locus_tag="VQ516_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2510588.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            67182..67866
                     /locus_tag="VQ516_000064"
                     /pseudo
     CDS             67182..67866
                     /locus_tag="VQ516_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            67875..68663
                     /gene="divIVA"
                     /locus_tag="VQ516_000065"
     CDS             67875..68663
                     /gene="divIVA"
                     /locus_tag="VQ516_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2510589.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            69011..71803
                     /locus_tag="VQ516_000066"
     CDS             69011..71803
                     /locus_tag="VQ516_000066"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2510590.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPAPEAALSFEDVVFTVERAAGEVCDRCRRIDPTTAERSYHAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            72213..72575
                     /locus_tag="VQ516_000067"
     CDS             72213..72575
                     /locus_tag="VQ516_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510591.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            73110..73802
                     /locus_tag="VQ516_000068"
     CDS             73110..73802
                     /locus_tag="VQ516_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2510592.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            73855..76266
                     /locus_tag="VQ516_000069"
     CDS             73855..76266
                     /locus_tag="VQ516_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2510593.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYSGNLFSY
                     KGQEYIEAARAILNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            76516..77217
                     /locus_tag="VQ516_000070"
     CDS             76516..77217
                     /locus_tag="VQ516_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510594.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            77219..79919
                     /locus_tag="VQ516_000071"
                     /pseudo
     CDS             77219..79919
                     /locus_tag="VQ516_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(80015..80506)
                     /gene="tpx"
                     /locus_tag="VQ516_000072"
     CDS             complement(80015..80506)
                     /gene="tpx"
                     /locus_tag="VQ516_000072"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2510595.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(80629..81558)
                     /gene="psaA"
                     /locus_tag="VQ516_000073"
     CDS             complement(80629..81558)
                     /gene="psaA"
                     /locus_tag="VQ516_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2510596.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(81590..82438)
                     /locus_tag="VQ516_000074"
     CDS             complement(81590..82438)
                     /locus_tag="VQ516_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2510597.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(82435..83157)
                     /locus_tag="VQ516_000075"
     CDS             complement(82435..83157)
                     /locus_tag="VQ516_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2510598.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        25167 a        15176 c        18384 g        24524 t
ORIGIN      
        1 tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg cattatcaat
       61 catgaactag gattctagtt ttgcaggaag tctactactt tccttattgt ctgtaaatct
      121 actgaccttg ttgtttatcc cagtcatggt ttctagttcg ggctcagagt ttcaaagtgg
      181 atggcaagag catcaattga ttgctgagaa ggttagtaaa acacttgata agacatttga
      241 taaggatgtc agagaaattc cgaccagtca gttttatcaa aaatttgtag atgagatggg
      301 aaggatttac tcaggaaatt tgatcctcca ggagctgata actgtgaatg gagcttataa
      361 agctacttat atcggtgaac tctctagcaa ctaatcattc tttaaaatga tgaccaataa
      421 gaggcttgat ttggtgaatc aagtcttttt tctctctttt actgccattt gtgataaaat
      481 agtatagaac gattttttac atgaatgata aaacagaggt aaatatgaca atcggtattg
      541 ataagattgg ttttgcgacc agtcaatatg tcttgaaatt acaagactta gcagaagcga
      601 ggggaattga ccctgaaaaa ttaagtaaag gactcttact caaggaattg agtattgcgc
      661 ccctaactga ggacatcgtg accttggcgg ccagtgctag tgactctatt ttaactgagc
      721 aagaaagaca agaagttgac atggtcattg tggctaccga gtcaggaatt gaccagagta
      781 aggctgcggc cgtctttgtg catggcttgc tgggcatcca gccctttgct cgtagtttcg
      841 agattaaaga agcctgctat ggggcgacag ctgccctcca ttatgccaaa ttgcatgtgg
      901 aaaattctcc ggagtccaag gtcttggtca ttgccagtga tattgccaaa tatggtattg
      961 aaactccggg agaaccaact caaggtgctg gaagtgtagc tatgttgatt acacaaaatc
     1021 cacgcatgat ggcctttaat aatgacaatg tagctcagac ccgtgacatc atggatttct
     1081 ggcgaccaaa ttactcgaca actccttatg taaatggtgt ctattctacc caacaatact
     1141 tggatagttt gaaaacgact tggcttgaat atcaaaaacg ctaccagctt actctggatg
     1201 attttgcggc tgtttgtttc cacttgcctt atcctaaatt agcgctaaaa ggcttgaaaa
     1261 aaatcatgga taagagcctg cctcaagaga aaaaagacct cttacaaaag cattttgacc
     1321 agtctattct ctacagtcaa aaggtgggga atatctacac aggttcactt ttccttggac
     1381 ttttgtctct cttggaaaat gcagatagct tgaaagctgg ggataaaatc gccctttata
     1441 gttacggaag tggagctgtg gctgagttct tcagtggtga attggttgaa ggatatgaag
     1501 cttatttgga taaagaccgc ttgaacaagc tcaaccaacg aactgcctta tccgttgcag
     1561 actatgaaaa ggtctttttt gaggaagtaa acttggatga aacaaactct gcccagtttg
     1621 ctggctatga aaatcaagat tttgccttgg ttgaaattct cgaccaccaa cgccgttata
     1681 gcaaggttga aaaataatga agataagttg gaatggattt tctaaaaaat cataccaaga
     1741 gcgcctcgag ctgttaaaag ctcaggcgct ccttagtcct gagagacaag ctagtctgga
     1801 gaaggatgaa cagatgagtg tgactgtggc agaccagctg agtgagaatg tagtgggaac
     1861 tttttctctg ccttattcgc tggttccgga ggtacttgtc aacggtcagg aatacaccgt
     1921 tccctatgtg acagaagaac cctctgtggt tgcggcggcc agctatgcca gcaaaatcat
     1981 caagcgtgca ggtggtttta ctgcacaagt ccatcagcgc cagatgattg ggcaggtagc
     2041 cctttatcaa gttgctaatc ctaaactagc gcaagagaag attgccagca agaaagcgga
     2101 gctcttggag tttgccaatc aagcctatcc ttctatcgtt aaacgtggag gtggggcgcg
     2161 tgatctgcat gtcgagcaga taaaaggcga accagacttt ctcgttgttt atattcatgt
     2221 cgatacccag gaagccatgg gtgccaatat gctcaacacc atgctggaag ccttgaaacc
     2281 agtcttagaa gaactcagtc agggacagag tctcatagga atcctgtcca actacgcgac
     2341 cgattctctg gtgactgcaa gctgtcgcat ggcctttcgc tacttgagcc gccaaaagga
     2401 tcaaggacga gagattgcgg agaaaattgc gttggctagt cagtttgcgc aggctgatcc
     2461 ttaccgagct gctactcata ataaaggaat ttttaatggt attgatgcga ttttgattgc
     2521 cactggtaat gactggcgtg ccatcgaagc tggggcccat gcctttgcca gtcgagatgg
     2581 acgctatcaa ggtcttagtt gctggacgct ggaccttgaa agagaagaat tggtcggtga
     2641 gatgaccctg cccatgcctg tagcgactaa gggtggctct atcggcctca acccacgtgt
     2701 agctttcagt catgatctac taggaaatcc ttctgccaga gaattagccc agattatcgt
     2761 gtccatcggt cttgcccaaa attttgcagc cctcaaagct ttggtaagta cgggcatcca
     2821 gcaaggccac atgaaactac aggccaaatc cctagctctc ctagctgggg ctagtgaatc
     2881 tgaagttgct cccctagtag agcgcctcat ctcagataaa acctttaacc tagagacagc
     2941 ccagcgctat ctcgaaaatt taagatcata aaaaactcag acgaatcggt ctgagttttc
     3001 ttgtgtttaa atactttttc ctggtaatag tgtaactggt aagaagtaac cagttgtggc
     3061 aacttccttg ccttcaatct tttgcaagag aagatcaata gtgagacaag caatctcttc
     3121 caaaggttgc ttgatagtag ccaattgagg gtagtaattt tcgataaagt aggtcccatc
     3181 atagccgatg accttgagct cttttgggac agaaatgccc aattcttgag cgattttaat
     3241 gaccagaata gctgtcaaat catccgaagc aaaaatggca tctggttttt cccgggtcaa
     3301 gatattcttg atttccattt cttttctgac gggagaaaag tcactggaaa cattgataat
     3361 aggagctttt gggagtacgg atgcaaaacc agcgtggcgt agtccggttg gcgaattgga
     3421 attgtcattc cctgtaatca tgatgatagt ctgggcacct gtcttgacca aggtttgggc
     3481 agcaagaacc ccaccagcat agttgtcaga ggagacgaca gggatgtctg gcgataggtt
     3541 tcggtcaaag gaaataatcg gcgctgtcac acgattgtag tcttcgattc ctaggttgtg
     3601 actaccagaa atgatgccgt ccacctgatt ggcttccaac atttcgatgt attcgcgttc
     3661 cttctcagaa tcatgttcac tgttgcagat gatggtcttg taaccatttt tgaagagttg
     3721 gtgttccaat ttatcaatca attctgcata gaaaacattg gaaatattag ggaaaatcaa
     3781 gccgattaac ttagctgatt ttccttgcag actacgagcc aggttgttgg gtttatagcc
     3841 caattctcgc atggcttcat tgactttttg gatggttttc tcagatagat accctttttt
     3901 attgataacc cgagaaacgg tagtaggact gacgcctgca agtttggcga catcagttag
     3961 ttttgcgacc ataatctaat tcatagtaag ttccagttgg gtttccagat ttgatcagga
     4021 taccattttg gtccgcatgt gggaagacac gaccagaaaa tactttttct cctttattga
     4081 tgaaaatttc aaagacagag ttatcgatga agattgtagc agtagtagcc tgattctcga
     4141 tagggcaaga acgagttgtc ccaaattctt gggcatactg ttctccagcc tggctacgat
     4201 ccactgtcac ttgaccgttt acaaggtcaa agttgattga aagtccctta ccttctttat
     4261 cagcaagtaa gacaatctcg ctctggctat tagcttccaa gttgagttca agttcgtaag
     4321 tgttcttggt ttgggaacgg tttgagaagg cttcttcaga agcacgaagg tccttaatag
     4381 cagcgactgg atactggtag agcttgtcgt ctttgatagt gagttccttg accaaagaga
     4441 aggttccttg gtggtcaaaa cggtcagatg ggtaagaaac atctggcaaa ccaagccagc
     4501 taactgctag agcacgccca tcaggagcgt tgaaggcttg agttgcatag gcttcgaaac
     4561 cgtaatccat gttttgaagt tgagacacat ctaccatttt ggcattttta gggtcaaagg
     4621 aagccccaat cttatacata tttggaaaga tattatcgta gtctagaact ttcttatcca
     4681 atccttgtgg acagtagaga aggacaggat gttcctctac aaagaccaga ttaggacatt
     4741 ccatcatgta ggcagtacgg tcgttagcaa agtcaaggtc gccaactgct tgccagtttg
     4801 tgtagtcgtt attgacagcc ttgtagagac ggacgaagcc ttttttctcc aagtcctgtc
     4861 caccgacgac agcatagtat tgacttttaa agttaaaaat ttgtggatcg cggaagtggt
     4921 cagtagagtc tgctggctgg tcaatcaaga tcttgtcaat ctttgtaatc ttaccctcct
     4981 tgtccatcaa agcaccgatc tggtatgggt gacggatcca gttttcatcg cgaacatttc
     5041 ctgtataaaa taggaataag ttatcgccaa attgcatggc agaaccagag taggcaccgt
     5101 ggctatctaa tggagtatct ggtaaaactt tgattccagt ttctttaaag tgaaccaaat
     5161 catcactttc tagctgtgcc caagatttca aaccgtgggc tgcaccaaaa ggaaaattct
     5221 ggtaaaagag gatccacttg ccatcaaagt aagaaaagcc atttgggtcg ttgagaagtc
     5281 ctgtttttgg ctcaacatgg taatgagtat gccatggaga ttgtgccata ttttccttta
     5341 tatgttgaat ttcttcttgc gtccaatctt gataaagtct gtaacgacgc tcagttgtcc
     5401 attccattca ttcctccaaa aatcttatca cttttaatag taactgtttt cgataaaaaa
     5461 tgcaagcctt ttatgttaaa aaagaggaaa aactgtcaaa cgattatcat aaaataaata
     5521 caggaaatca atcaaatttt tacgaaaatg tgaaagataa tgaaataaaa gactttttaa
     5581 ccaaggtttc aaacttattt ttcaaggaat tcctgatgaa acagtcaaaa acaatcaatc
     5641 taaaacttgc ccactaagtg gatagaatat tttttctgca aaacgcttga catattttta
     5701 aaacatgata aaataatagt cgtaagggcg aataaaatcg ctttcaaaca agaacaaaat
     5761 gttatataag gagatttttg caaatgaaca atcaggaaat tgcaaaaaaa gtcatcgatg
     5821 ccttgggcgg acgtgaaaat gtcaatagtg ttgcccactg tgcgactcgt ctacgtgtca
     5881 tggtcaaaga tgaagagaaa atcaataaag aagtgattga gaacttggaa aaagttcaag
     5941 gtgctttctt taactcaggg caataccaaa ttatctttgg tacaggtaca gttaacaaaa
     6001 tgtacgatga agttgttgta cttggcttac caacatcatc taaggatgac atgaaagcag
     6061 aagttgctaa acaagggaac tggttccaac gtgctatccg tactttcggt gatgttttcg
     6121 ttccaatcat cccagttatc gtagcgacag gtctcttcat gggtgtgcgt ggtcttttca
     6181 acgctcttga aatgccactt ccaggtgact ttgcaactta cacacaaatc ttgacagata
     6241 cagccttcat catcttgcca ggtttggttg tgtggtcaac cttccgtgta tttggtggaa
     6301 atcctgccgt tggtatcgtt cttggtatga tgcttgtctc tggctcactt ccaaacgctt
     6361 gggcagttgc tcaaggtggt gaagtaacag cgatgaactt ctttggtttc atccctgttg
     6421 ttggtttgca aggttccgtt cttccagcct tcatcatcgg ggttgtcgga gctaaatttg
     6481 aaaaagctgt ccgcaaggtt gttccagatg tcattgacct cttggtaaca ccattcgtga
     6541 cacttttggt catgtctatc cttggactct ttgtcattgg accagttttc cacgttgttg
     6601 aaaactacat ccttattgct acaaaagcga ttcttagcat gccatttggt cttggtggtt
     6661 tcttgattgg tggggttcac caattgatcg tcgtgtcagg tgtgcaccac atcttcaact
     6721 tgcttgaagt gcaattactt gctgctgacc atgctaaccc attcaacgct atcatcacag
     6781 ctgctatgac agctcaaggt gctgctactg ttgcggttgg tgttaaaaca aaaaatccaa
     6841 aactgaaaac acttgctttc ccggctgctc tttctgcctt cctaggtatt acagagcctg
     6901 ctatcttcgg ggtgaacttg cgcttccgta aaccattctt cctttcattg attgctggtg
     6961 caatcggtgg tggattggct tctatccttg gacttgctgg tactggtaat ggtatcacca
     7021 tcatccctgg tacaatgctt tatgttggta acggacaact tccacaatac cttcttatgg
     7081 tagctgtatc atttgccctt ggttttgctc ttacttacat gtttggttac gaagatgaag
     7141 tagacgcaac tgcagctgca aaacaagctg aagtggctga agaaaaagaa gaagttgcgc
     7201 cagcagctct tcaaaatgaa acacttgtaa ctcctatcgt cggtgatgtt gtcgctcttg
     7261 ctgatgtcaa tgacccagtc ttctcaagtg gagctatggg acaaggtatc gctgtgaaac
     7321 caagtcaagg cgtggtctat gcactagctg atgctgaagt ttcaattgcc tttccaacag
     7381 ggcacgcttt tggtttgaaa acaagaaatg gtgctgaagt tttgattcac gttggtattg
     7441 acactgtatc tatgaacggt gacggttttg aagcaaaagt tgctcaaggt aataaggtga
     7501 aagctggcga tgttcttgga acatttgact caaacaaaat cgctgcagct ggacttgatg
     7561 atacaacaat ggttatcgtt acaaatacag ctgactacgc ttcagtagct ccagtcgcaa
     7621 caggttcagt ttcgaagggg gatgctgtga tcgaagtgaa aatctaatca atcctctcta
     7681 atgtgaaaac gaacaaatga catgtttgtt cgtttttatt agatagtaag atttacagag
     7741 gaaaatctaa aaaatagaga catttagact ttcgaagtat gctataataa agaaaataaa
     7801 aacaagaggt ttatcatgac aaaattatat ggaagcttgg aagcgggcgg tacaaagttt
     7861 gtctgtgctg tcggtgatga aaactttaac gttgtagaaa aaacacaatt tccaacaaca
     7921 actccaatcg aaacaatcga taaaaccatt gagttcttct caaaattcga taaccttgct
     7981 ggtcttgcag ttggttcatt tgggccgatt gatattgaca aaaactcaaa aacttatggc
     8041 tttatcacga cgactccaaa accaaactgg gcaaatgtgg acttgcttgg tgcccttcgt
     8101 cgcgccctaa acgtgccaat gtacttcaca acagacgtaa acagctctgc ttacggtgaa
     8161 atggttgctc gtaacaatgc tggtggtcgt atcgaaaact tggtttacta cacaatcggt
     8221 acaggtatcg gtgcaggcgt catccaacgt ggtgagttta tcggtggtgt gggtcaccct
     8281 gaaatgggtc attattatgt tgctagacac ccaatggata ttgaaaaaga gtttaagggt
     8341 gtttgtcctt tccataaggg atgtctagaa ggttatgcag ctggtacaag tttggaagct
     8401 cgtacaggtg tacgtgggga aactattgaa ctcaacaacc ctgtttggga tgttcaagcc
     8461 tactatatcg ctcaagctgc ggttaatgcg acagtgactt tccgcccaga cgtgattgtc
     8521 tttggtggag gggtcatggc tcaacaacat atgctggacc gtgtccgtga gaaatttaca
     8581 tctcttctta atggttacct accagtacca gatgtgcgtg actatatcgt gacgccagca
     8641 gtcgcaggaa atggttctgc cacacttggg aactttgttc ttgcaaaaga agtttcaaaa
     8701 taaagcacaa aatccgcttg ggaaaccaaa cggattttgt ggttgaaaac aatatttttg
     8761 agcctttcct taagtgatta cggacggtag cgactttctt cgaaattcca tacctaaact
     8821 ttgagcctag tgtctcaaag tttccgaata cctgaaacca ttagatttca ggtgtttcta
     8881 tcacggcgga aaggctaggt aagtcctcgc ctcactatcg aaaaatatgc ataatttcca
     8941 taccaaagta tatttagtat caagctatag ttaatctacg ctttaaaaat ccacttggga
     9001 aaccaaacgg attttttacg tgtcaaagca tttgccagaa aaagtcaata atataggtca
     9061 gaccgtaggt ataaaccacg agggtaaagg gtttggtcag aatgatgata gtcatgtagc
     9121 gcttgaaact catcttggtc agggcggcca gcatacagag aaagtcagct gggctaatgg
     9181 gccaaatcat cataaaaata aagaagcggt caaaacgatt gcccttatct agccagccga
     9241 tgtacttgtc gtaggtgcgc ttgctgacga cagactggac aaaggcagct ccgtataggc
     9301 gcactagata aaagataatg gcacagccaa tcacgatgcc gatatagttg tagatagtcc
     9361 cgatgatgtg cccgtagata aagaccccag ccaccgaggt caaggcccct ggaatgatag
     9421 ggacgacagt ctgtaaaatc tgtaaaaaga taaagagagg tggaccccag atgcctgcct
     9481 gctggataaa ggcagagagg gtttccttgg attgtaaaat cccagcctga taagcccaaa
     9541 tacagaaaat caaactccca accccaccga caatcgatga gatattgata actcgctgca
     9601 gaaggggaga aacagctttc atttgtttat cctgtgacat gtaaactcct catagatagt
     9661 atatttctac tataccatat tttggaggag aaagggggaa tgagtctttt ttgataagga
     9721 atagaaagta aatagccctt cgagacttag aataggtctg tcaactaaag gttatatgca
     9781 ttctaggttt cgaataatcg caattccata aatttaaaag tattctaagt ttcgaatagc
     9841 ttatctgaga taattttgag gcattcgcgc tttcgaatag tcattttccc taaagtttat
     9901 atgtattctg aagctagaaa gactaaaata taaaagtagt aagagtctct ctctgatgga
     9961 cgtaatttat ggctagtatg aatcctttga attgttagtt aattttaata ttaatcagtt
    10021 ctgaaataat tactaaatat agttagatgg tgagttaatt attgctaaat ttcaagttca
    10081 agttagtcat ccagaagtct tctctggggg acttgtagtt caagcatttt ttaggatagt
    10141 tgttaatcca cttttcgata aatgcgactt cctttgtagt tgttttctta gttccctgag
    10201 gtaaccatct atgaatgagc ctgttgtgat tttcattaga tcctatagtg atgtgcataa
    10261 tagatatagc agattgaaac tagaatagta tacatctact tctaaaacat tgttagaaat
    10321 caatttgact gtcctgatca atttgtcctg tttttatttc attttactat ataatcctta
    10381 gaaaacatat cggacaagcg gttgaattcc gttccgtcca ttagtagata aaggattact
    10441 ccttcttgaa atgacagatg aacttgtttc ttataaaaat gttgtctgaa actattagcc
    10501 tttttccaga agttttagag gaagaaaact ttacgaggaa aaaggagtta ctcaaataac
    10561 aattcaggat taaaaataga cagttgagga gcggaaggta taaattaagt ttgctactgt
    10621 ataatggatt tatcactaat actcttcgaa aatctcttca aaccacgtca gcttcgcctt
    10681 gccgtagata tagttactga cttcgtcagt tctatccaca acctcaaaac agtgttttga
    10741 gctgacttcg tcagttttat ctgcaacctc aaagctgtac tttgagcagc ctacggctag
    10801 cttcctagtt tgctctttgc ttttcattga gtataaaagt gctccaggcc atattttaca
    10861 cagcaatagt cttctaaaaa tggacaaaaa cattgtaaag gctatcaaaa aggagtataa
    10921 tgagtgcaag aaatttcgga ggtgaaagat gctagaagta agaagtctag agaaaagttt
    10981 tggctccaag caagttttgt ttggtattga ctttcaagcg cgaccaggtc gtattttggg
    11041 actagtcgga aaaaatggtg ctggaaaaac aacgattttc catagtattt tgaaattcct
    11101 agaatatcag ggagaaatcg gtctggatgg tcaggatatt cgtcaggaga cctatgctcg
    11161 gattggttat ctgcctgaag aacgcagtct catgcctaaa ttgacagttc ttgaacaagt
    11221 tcgctacttg gcgactctaa aaggcatgga tgccaaagaa gtcaaagaaa aactccctca
    11281 atggatgaag aggttggaag tgaaaggaaa gctgacagat aaaatcaaga gtctgtcaaa
    11341 aggaaatcag cagaagattc agctcattat tactctgatt catgaaccag acctgattat
    11401 cttggatgag ccttttagtg gattggaccc agttaataca gaattgctca aacaagtcat
    11461 ttttcaggaa aaagagcgcg gagcaaccat tatcttttct gaccatgtca tgaccaatgt
    11521 tgaggaactt tgtgacgata ttctgatgat ccgagatggc cgtgtggtct tgcacggacc
    11581 agttcaggat gtccgcaatc aatacgggaa aacgcgtctc tttgtttcaa gtgaacgaag
    11641 caaggaagaa ttggaaaatc ttcctcatgt caaacaggtg agcttgacca aacaaggcag
    11701 ttggaaattg attttggagg atgagagcgc tggaagggaa ctcttcccaa tcttgactca
    11761 aggtcaatat atcgcaacat ttgaccaaca agcgccaact atcgatgaaa tctttaaact
    11821 agaatcagga gtggaagtat gagaaatatg tgggttgtaa tcaaggaaac ctatcttcga
    11881 catgtcgagt catggagttt cttctttatg gtgatttcgc cgttcctctt tttaggaatc
    11941 tctgtaggaa ttgggcatct ccaaggttct tctatggcta aaaataataa agtggcagta
    12001 gtgacaacag tgccatctgt agcagaagga ctgaagaatg taaatggtgt taacttcgac
    12061 tataaagacg aagcaagtgc caaagaagca attaaagaag aaaaattaaa aggttatttg
    12121 accattgatc aagaagatag tgttctaaag gcagtttatc atggcgaaac atcgcttgaa
    12181 aatggaatta aatttgaggt tacaggtaca ctcaatgaac tgcaaaatca gcttaatcgt
    12241 tcaactgctt ccttgtctca agagcaggaa aaacgcttag agcagacaat tcaattcaca
    12301 gaaaagattg atgaagccaa ggaaaataaa aagtttattc aaacaattgc agcaggtgcc
    12361 ttaggattct ttctttatat gattctgatt acctatgcgg gtgtaacagc tcaggaagtt
    12421 gccagtgaaa aaggcaccaa aattatggaa gtcgtttttt ctagcataag ggcaagtcac
    12481 tatttctatg cgcggatgat ggctctgttt ctagtgattt taacgcatat tgggatctat
    12541 gttgtaggtg gtctggctgc cgttttgctc tttaaagatt tgccattctt ggctcagtct
    12601 ggtattttgg atcacttggg agatgctatc tcactgaata ccttgctctt tattttgatc
    12661 agtcttttca tgtacgtagt cttggcagca ttcctaggat ctatggtttc tcgtcctgag
    12721 gactcaggga aagccttgtc gcctttgatg attttgatta tgggtggttt ttttggagtg
    12781 acagctctag gtgcagctgg tgacaatctc ctcttgaaga ttggttctta tattcccttt
    12841 atttcgacct tctttatgcc gtttcgaacg attaatggct atgcgggggg agcagaagca
    12901 tggatttcac ttgctattac agtgattttt gcggtggtag caacaggatt tatcggacgc
    12961 atgtatgcta gtctcgttct tcaaacggat gatttaggga tttggaaaac ctttaaacgt
    13021 gccttatctt ataaatagaa gagccttgcg aaagcgaggt ttttaaaggt gaaaagggtg
    13081 gaatcacgaa cctaatgatg tacacattat gtaaacaggt tgcttgaaaa ctcaattgta
    13141 tagtgaactg aatctggaat aggataatat tacttctaaa acattcttat aaattgattt
    13201 gaattccaaa atcccatcgt tccgttctta tttcattttg cgatatagtg aaaggaaata
    13261 agtcagctca caaaaaacgc ataatatcaa ggatttttaa cacttgatac tatgcgtttt
    13321 ctaattttaa agactttttg cctggtctct tttgttctat gagtatgtta catatctagg
    13381 taaaaattat ttaaagcaaa ttaaccttat ttctcaagat gtagtaggtt ccgagataaa
    13441 agatagctat gaatatgagt tctttaacaa tggctatacc tgctcccata tagaaatggt
    13501 cattgagtcc taccagtgaa ttgacataga aattagaact aagattgaag aaaagttcaa
    13561 taaatccaat gacgatttgg ataccaatgt aggctgcaac agcgagtgct gtacggtatt
    13621 cattgaaaag ctgtccaatg gaaatagcca ggtagatgca gaggattcct gaaatggtat
    13681 ttagtaggaa ggatatacct gtaagaaaga tctgagggag atgtgtttct acaaatgtaa
    13741 tcacataaga aagaggaatc cattctggag ctgttaaagc cagaataata acagcactta
    13801 gagccaatac agcggtgcta atcaatgacc agataaaggc accgattagt ttggctgtga
    13861 tgatatggtg ttcagaaact ggcaaggtca aagtcagata gccttgtcgg tcgtagacac
    13921 tacctttgaa gcgtttaata atcaagaaaa tggttgaaat cgcaagtgta agcatcaagc
    13981 caccaaagac tgtagctaga aaaagtagca tagtagcctg actttcttgg taaggtagat
    14041 ttttaaagcc ttgtgtctgt attccgatga gggcagaaag gactagcacg gctgcgtaga
    14101 gggctaaata ccacttgtta acatttttaa attcgtagtg aactaaattc caaaacataa
    14161 ataatctcct ttgcttaggc cttaaattcc tgacggaaga gttggtcaat ggattcacct
    14221 gactcgtagc gaatatcatc tacatttcct tgacggacga cttttccgtc ttttaggaag
    14281 acaatttcat ccaagattgg ctcgatatca gaaatcaagt gggtagaaat caaaacggta
    14341 gaagttggtg agtagttgtt gataatggta ttgaggatat aagcacgggc tgctggatcc
    14401 accccaccaa tgggttcatc caaaacatag agacgagcat cacggctcat aaccaaaatc
    14461 agttgaacct tttctttgtt tccttttgat agtttcttga gacgactatt ttcatcaatg
    14521 cccaggtctg caagtagatg atgagcgcgt tcaagattga aatctttata gaaggtcttg
    14581 aagtaggtta gggcttcttt gaccttcatt tgctcattga gataggtcgt atcaggcaaa
    14641 taagctacaa cggccttggt tgctgggctt gggtccatgt cgttgatgag gacacgtcct
    14701 tgatctggtt gtaagaggcc attaattagt ttaatcaggg ttgtttttcc tgagccgttt
    14761 ggcccaagaa ggccgacaat ttttccagct ggaatgtcaa gagaaacatt ttcaagggct
    14821 ggtgttgctc catatgattt ggatacattt tcaaatacta gtaatgacat aggcttaaac
    14881 tcctttaata taatcactga ctacgcctgg tagttcttct ttttcatagc caaaatggat
    14941 catggaggaa acgaagtgtt ccaattcttc ttctgataat tgtttgcgtg actgagcgat
    15001 tagctcctta tccttagtca caaatcgtcc agttgttcgc ttgctgtaga caaatccttc
    15061 tcgttcaagg tctgataagg ctctttggat ggtattggga ttgacaccag cctcgctagc
    15121 tagctccctc acggttggaa gttgttgatt gggttccagt gtatgggaaa caatctgaag
    15181 cttgattttc tccataatct gtaaatagat gggttttttg ttgtcaaatg tccaggacat
    15241 cttagtctcc tttctcttat ccttttgtct taactaaata atacaacaaa acaaaaaact
    15301 tgtcaagcaa aaagaagaat tgttttggga atcgcttaaa aaatggtata atgaaaagaa
    15361 aacgataagg agggaaagga tgcgttgtcc aaaatgtggg gctaccaagt caagtgttat
    15421 cgatagtcgc caagcagaag aagggaacac cattcgtaga agacgtgagt gcgacgaatg
    15481 ccaacaccgt tttacaacct acgaacgagt agaagaaaga accttagtgg ttgttaaaaa
    15541 agatggtaca cgggaacaat tctccagaga taaaatcttt aatgggatta tccgctcagc
    15601 ccagaaacgt cctgtgtcaa gtgatgaaat caacatggta gtcaatcgta tcgaacagaa
    15661 actccgtggt cgaaatgaaa atgaaattca aagtgaggac attggttcac tcgtcatgga
    15721 ggagttggct gaattggacg agattaccta tgtacgtttt gctagtgtct atcgtagttt
    15781 taaggatgtc agtgagttag agagcttgct ccaacaaatc acccagtcct ctaaaaagaa
    15841 aaaggaaaga taaatgaagc caattgaccg tttttcttat ctaaagaata atcgggtgtc
    15901 gcaagatacc tcatctctgg tacagtgcta cctcccgatt atcggtcagg aggcactgag
    15961 cctttatctt tatacaatca gtttttggga taatggtaga aaggaatatc ttttttcaag
    16021 tatcctcaat catctcaact ttgggatgga tagactgata aaatcattga aaatcttatc
    16081 tgcttttaat ctcttgaccc tctatcaaaa gggggatgtt tatcagctag ccctccatgc
    16141 tcctctatct agtcaagact tcttggagca tcctgtttat cgcagactct tagagaaaaa
    16201 gattggcgac tctgctgtgg aggatttgaa agttgaaagt gctgatggtg aagaaatacc
    16261 tgtctcactc aatcaagtct ttccagaatt ggcagaacta ggcagtcaag aagaccttgg
    16321 tctcaagaag aaagtggcca atgattttga cttggaacat tttcgccagc tgatggctcg
    16381 agatggactt cgttttgcgg atgagcagtc cgatgtctta aacctctttg ctattgccga
    16441 ggagaagaaa tggacttggt ttgaaaccta tcaattggcc aagtcaacag ctgtttctca
    16501 ggttatttca accaaacgca tgcgtgaaaa aattgctcaa aaaccagttt cctctgactt
    16561 taatcctaag gaagcaacca ttatcaagga agctaaaagt aagactgccc tgcagttctt
    16621 agcagaaatc aagcaaacac gcaaggggac cattacccaa acagaaagag aacttttgca
    16681 acagatggct ggcttgggct tgctggacga agtcatcaat atcattctct tgttgacctt
    16741 taataaggtg gattcggcaa acatcaatga gaaatatgcc atgaaggtgg ctaatgacta
    16801 tgcctatcaa aagatacatt cagcagaaga ggccgtttta cgaattcgtg agcgagggca
    16861 gaaaacaaaa acacaaaaac agaatcagac tgccccagca aaaaccaata ttcctaaatg
    16921 gagtaaccca gattataaga atgaaaccag cgaggaaact cgtctggaac tagaacgtaa
    16981 gaaacaagaa ctattagctc gattagaaaa aggaggagat tagatggaaa gtgtcggaga
    17041 cgtactcaaa cgtcaaccca gccgttttta ttatcaagat ttggtccaga aaatcatgaa
    17101 ggaccctgat gttgcggctt ttatccagca agaatccttg actccagagg aattaaatcg
    17161 cagtatctcc aagtttaatc agtacatcac cgagcgtgac aagtttctcc gtggggatac
    17221 ggattatatt gccaaaggct acaagccgat tttggttaag aatcatggct atgcagatgt
    17281 ttcatatgaa gaaactcctg agctaatcgc ggctgaaaaa gaagcggcta ttaagaatcg
    17341 tctcaagcta atcaatctac cagccagtct caaaaaagct agtttggctc aagttgattt
    17401 ggatgatttg ggacgcttgc cagtttttga aaaactatta gcctttgtgg agcaatatcc
    17461 agctattcga aaaggtcttt acttatatgg agactttggt gtgggtaaaa gtttcatggt
    17521 ggcggcctta gctcatgatt tatcagaaaa acgtggtgtt tcatcaactc tcctccacta
    17581 tcctagcttt gtcattgatg tcaaaaatgc tatcagtgat ggcaatgtta agaccttggt
    17641 ggatgagatt aagctgtctg aggtcctgat tttagatgat attggcgccg agcaatcaac
    17701 cgcttgggtg cgtgacgaaa tcctgcaggt cattctccaa tatcggatgc aggaaaattt
    17761 accgaccttt ttcacatcca acttcaactt tgaagatttg gagaagcatt ttgcgaaagt
    17821 gaaacatgga aatgacgaaa cctgggaagc cagacgggtc atggaacgca tccgttattt
    17881 ggctgaggag actcgtttag aaggagtaaa ccgtcgatga cagaaaccat taaattgatg
    17941 aaggctcata cttcagtgcg caggtttaaa gagcaagaaa ttccccaagt agacttaaat
    18001 gagattttga cagcagccca gatggcatca tcttggaaga atttccaatc ctactctgtg
    18061 attgtggtac gaagtcaaga gaagaaagat gccttgtatg aattggtacc tcaagaagcc
    18121 attcgccagt ctgctgtttt ccttctcttt gtcggagatt tgaaccgagc agaaaaggga
    18181 gcccgacttc ataccgacac cttccaaccc caaggtgtgg aaggtctctt gattagttcg
    18241 gtcgatgcag ctcttgctgg acaaaacgcc ttgttggcag ctgaaagctt gggctatggt
    18301 ggtgtgatta tcggtttggt tcgatacaag tctgaagaag tggcagagct ctttaaccta
    18361 cctgactaca cctattctgt ctttgggatg gcactgggtg tgccaaatca acatcatgat
    18421 atgaaaccga gactgccact agagaatgtt gtctttgagg aagaatacca agaacagtca
    18481 actgaggcaa tccaagctta tgaccgtgtt caggctgact atgctggggc gcgtgcgacc
    18541 acaagctgga gtcagcgcct agcagaacag tttggtcaag ctgaaccaag ctcaactaga
    18601 aaaaatcttg aacagaagaa attattgtag aaagtgagaa attatggccc taccaactat
    18661 tgccattgta ggacgtccca atgttgggaa atcaacccta tttaatcgga tcgctggtga
    18721 gcgaatctcc attgtagaag atgtcgaagg agtgacacgt gaccgtattt atgcaacggg
    18781 tgagtggctc aatcgttctt ttagcatgat tgatacagga ggaattgatg atgtcgatgc
    18841 tcctttcatg gaacaaatca agcaccaggc agaaattgcc atggaagaag cagatgttat
    18901 cgtctttgtc gtgtccggta aggaaggaat taccgatgca gacgaatacg tagcccgtaa
    18961 gctttataag acccacaaac cagttatcct cgcagtcaac aaggtggaca accctgagat
    19021 gagaaatgat atctatgatt tctatgctct cggtttgggt gaaccattgc ctatctcatc
    19081 tgtccatgga atcggtacag gggatgtgct agatgcgatc gtagaaaatc ttccaaatga
    19141 atatgaggaa gaaaatccag atgtcattaa gtttagcttg attggtcgtc ctaacgttgg
    19201 aaaatcaagc ttgatcaatg ctatcttggg agaagaccgt gttattgcta gtcctgttgc
    19261 tggaacaact cgtgatgcca ttgataccca ctttacagat acagatggtc aagagtttac
    19321 catgattgat acggctggta tgcgtaagtc tggtaaggtt tatgaaaata ctgagaaata
    19381 ctctgttatg cgtgccatgc gtgctattga ccgttcagat gtggtcttga tggtcatcaa
    19441 tgcggaagaa ggcattcgtg agtacgacaa gcgtatcgca ggatttgccc atgaagctgg
    19501 taaagggatg attatcgtgg tcaacaagtg ggatacgctt gaaaaagata accacactat
    19561 gaaaaactgg gaagaagata tccgtgagca gttccaatac ctgccttacg caccgattat
    19621 ctttgtatca gctttaacca agcaacgtct ccacaaactt cctgagatga ttaagcaaat
    19681 cagcgaaagt caaaatacac gtattccatc agctgtcttg aacgatgtca tcatggatgc
    19741 cattgccatc aacccaacac cgacagacaa aggaaaacgt ctcaagattt tctatgcgac
    19801 ccaagtggca accaaaccac caacctttgt catctttgtc aatgaagaag aactcatgca
    19861 cttttcttac ctgcgtttct tggaaaatca aatccgcaag gcctttgttt ttgagggaac
    19921 accgattcat ctcatcgcaa gaaaacgcaa ataaaaaagt agaatctgga atgacagttc
    19981 cggatttttt tgatataata aaataataga aaacgctatc aaaagaagga gggtgatgtc
    20041 ggtgatggaa catttgttta aattcttact tttagcaccg tacttttatt ttgataactg
    20101 gattgagaag gccaacagaa atagtaagtt ttttccaatt ttttattatt tttactggtt
    20161 ttacatcccc ttctattctc tttttagcct tgcttggaca gttgtttcag ttctgttttt
    20221 caataccgtc ttgagaaatg tgacagatat caagttatgg ggcatttggt ttctttttat
    20281 tctgctagct attggtatga attggttaac ttattcctgt ttcaaagaaa tgtttcgctt
    20341 gagacaggaa ctagggaagt ctaagggtgg aaggcattga tttatatcgg tttctatttg
    20401 taggaggtgg cttatggtac ctctaaaatc atttattaca cgctattcca aggtttatat
    20461 tggtaataac atttaataga acgaaactaa agaaaacaat ttaaaagagg aggtttgtga
    20521 tggaatactt gtttaaaatg ataatcttat taccctgttt ttactttttc agctggattg
    20581 aaaaggcaaa cagagatagt aagattttcc caatttttta ctatttttac tggatttaca
    20641 tcaccctcta tgctcttttt agccttgctt ggacagtttt ttcagttctg tttttcaata
    20701 tcgtcttgag aaatttgaca gatatcaagt tatggggtat ttggctatta ttactactta
    20761 tcgcttttgc tagtgactgg ttagactata tctgtttcaa aaaaatgctt gacttgagac
    20821 gggaactagg gaagtctaaa ggcggaaggc attgatttat actcttcgaa aatctcttca
    20881 aaccacgtca acgtcgcctt ggattatata tgtaactgac ttcgtcagtc ttatctacaa
    20941 cctcaaagca gtgctttgag caacttgcgg ctagtttcct agtttgctct ttgattttca
    21001 ttgagtatta tattactttc tatttgtagg aggtggctta tgaagattcc tctcttaact
    21061 ttggcaaggc ataaatttgt ttatgtcttg cttactttgc tttttcttgc tttggtttat
    21121 cgtgatgttt tgatgactta tttctttttt gatattcatg cgcccgatct agctaaattc
    21181 gatggacaag caattaaaaa tgacttatta aaatcagcat tagattttcg tattctccag
    21241 ttcaatctag gtttttatca atcatttatt attccaatca tcattgtttt gctaggtttt
    21301 caatatattg agctgaaaaa taaagtttta cgattgagta ttggaagaga agtgagctat
    21361 caagggttaa aaagaaagtt gactttgcaa gttgcaagta tcccttgttt gatatattta
    21421 gtgactgtgc tgataattgc aattataacc tatttctttg ggactttttc tcctcttgaa
    21481 tggaattctc tattttctga tgggagtggt ttacaaagac tcctagatgg agagataaaa
    21541 agctatttgt tctttacttg tgtcctacta atcggtattt tcatcaatgc aatctatttt
    21601 ttacaaatag ttgattatgt ggggaatgtg actcgttcgg caatcaccta tttgatgttt
    21661 ctttggcttg gttctatgct gctctatagt gccttgcctt actatatggt tcctatgacg
    21721 agtttgatgc aagctagcta tggggatgta agtttgatga aactctttac tccttatatc
    21781 ctttatgttg tcccttatat ggtacttaaa aaatatgaag ataatgttta agaattttaa
    21841 caatatttgg ctaaagagaa agatcgtttt actacttcgt atagttctga tgatgatttt
    21901 gataaactat ctattgtcaa cagcggttca aaagcaggat gctgttatct ttttcaagag
    21961 agaattgatt tcaatttttt cctataatga ctattctgaa gcgaatttag aaatccccaa
    22021 actcttgtta aacctttcgc ttttcatggt aggatggctc tctgtcattt tacttgaaag
    22081 tgatttggca gaccattacc atcacttgat tcgctatcaa tcaagctcct ttttcgatta
    22141 tacaaggaaa cgattggttg tcatttctaa attttttact caagatttgt ttgtctggtt
    22201 tcttggttta cttccgctag gaattcattt caaaacagtc gcacttttct ttttacttgc
    22261 tcagttaatg atgttgtact tactactgtc ttatctgata gcactgatta gtgcgggcgc
    22321 tggtttttct ttttttctct attttttggc atttgtggga caagaatgga tgatggatca
    22381 tattgtaaca gtgtatttag tactcttaag cttattagtt atgttgattg ttagtcgctt
    22441 ggaagagaaa tttaagaaag gataaacgat gagacttgaa attataaatg gacagaaaat
    22501 ttatgggaaa agacctattt taaatcagtt gaatttggtg tttcaatcag gaaaaattta
    22561 tggacttaaa ggtgataatg gatctggcaa gacggttctt ttaaagatac ttgctggtta
    22621 tattaagctt gacaaaggaa aagttcttca agatggtaaa gtttacgggg taaaaaatca
    22681 ttatattcag gatgcaggaa ttttaattga aaaagtcgag tttttatctc atttatccct
    22741 gagagaaaat ttggaactgt taaggtattt ttcatctaaa gttacggaaa aaagaattgc
    22801 ctattggatt caatactatg atttacagga atttgaagac gttgaatacc gtcatttatc
    22861 cttgggaaca aagcaaaaaa tggccttgat tcaagccttt atttcctctc cttctatact
    22921 ctttctcgat gaacctatga atgctttgga tgagaagagt gtgaggttaa ccaaacaggt
    22981 cattttatct tacctgaaaa aagaaaatgg tctggttatc ctgacgtcgc acatatcgga
    23041 agatatttca gacctttgta cagatgtatt agttgtcgaa aatggacata tacaatatgt
    23101 aaaggatata caatcctagg agatggctta tggcacatct aaaatcattt attacacgct
    23161 attccaaggt ttatattggt ttagttctgc tgatctggct gtctttcttc tttatccctt
    23221 gggatagagc gattctgggg gtaaggattg acatcttcat catgcagaaa atcttgctag
    23281 cttttggaat tctgtccatt ctcatggcct tgctgtccaa gaaagtcagt ctctttgttt
    23341 ttggactgat ttgctgtctt tctctttgga ttaacttatt tatcacattt gccattttgc
    23401 cgatttttgg caattaaaca gtcataaaag tcggagaggt tagcttgaaa actaacctct
    23461 ttttcctttt caaaatgggg attcttcctt gaaaataatc agtaattgtg ctaaaattaa
    23521 aggaacattc taaaatattc ggaatttaaa gtaaggaaaa acatggctaa tattttaaaa
    23581 acaattatcg aaaatgataa aggagaaatc cgtcgtctgg aaaagatggc tgacaaggtt
    23641 ttcaaatacg aagaccaaat ggctactttg actgacgacc aactaaaagc aaaaacagtt
    23701 gaatttaagg aacgttatca aaatggagaa tcactggatt cattgcttta cgaagcattt
    23761 gcggttgtcc gtgaaggtgc caaacgtgtc ctaggtctct tcccttataa ggttcaggtc
    23821 atggggggga ttgttcttca ccatggtgac gtgccagaga tgcgtacagg ggaagggaaa
    23881 accttgactg cgaccatgcc ggtatacctc aatgcccttt caggtaaagg ggttcacgta
    23941 gttacggtta atgaatacct gtcagaacgt gacgcgactg agatgggtga attgtactct
    24001 tggcttggtt tgtcagtagg gattaacttg gctaccaaat ctccaatgga gaaaaaagaa
    24061 gcctatgagt gtgatattac ttactcaact aactcagaaa tcggatttga ctaccttcgt
    24121 gacaacatgg tcgttcgcgc cgaaaacatg gtacaacgtc cgcttaacta tgccttggtc
    24181 gatgaggttg actctatctt gattgacgag gctcgtacac ctttgattgt atcaggtgcc
    24241 aatgcggttg aaaccagtca gttgtatcac atggcagacc actatgtaaa atctttgaac
    24301 aaagatgact acatcatcga tgtgcagtct aagactattg gtttgtctga ttcagggatt
    24361 gacagggctg aaagctactt caaacttgaa aacctctatg acatcgaaaa cgtggctttg
    24421 actcacttta tcgataacgc ccttcgtgcc aactacatca tgcttctcga tattgactat
    24481 gtggtgagcg aagagcaaga aatcttgatt gtcgaccaat ttacaggtcg taccatggaa
    24541 ggtcgtcgtt attctgatgg attgcaccaa gctattgaag ccaaagaagg tgtgccaatc
    24601 caggatgaaa ccaagacatc tgcctcaatc acgtaccaaa accttttccg tatgtacaag
    24661 aaattgtctg gtatgacggg tacaggtaag actgaggaag aagaattccg tgaaatctac
    24721 aacattcgtg ttattccaat cccaacaaac cgtcctgttc aacgtattga ccactcagac
    24781 cttctttatg caagtatcga atctaagttt aaagcggttg tcgaagacgt taaggctcgt
    24841 taccaaaagg gtcaacctgt cttggttggt acagtagcgg ttgaaactag tgactacatt
    24901 tctaagaaat tggttgcagc tggtgttcct cacgaagtct tgaatgccaa aaaccactat
    24961 agagaagccc aaatcatcat gaatgctggt caacgtggtg ccgttaccat cgcaaccaac
    25021 atggcgggtc gtggtaccga catcaagctt ggtgaaggtg ttcgtgaact tggaggactt
    25081 tgtgttattg gtacagaacg tcatgaaagt cgtcgtatcg ataaccagct tcgtggacgt
    25141 tcaggtcgtc aaggagatcc aggtgagtca caattctacc tatctcttga agatgatttg
    25201 atgaaacgtt ttggttctga acgcttgaag ggaatctttg aacgtttgaa catgtctgaa
    25261 gaggcgattg agtctcgcat gttgacgcgt caggttgaag cagctcagaa acgtgtcgaa
    25321 ggaaataact acgatacccg taaacaagtc cttcaatacg atgatgtcat gcgtgaacaa
    25381 cgtgagatta tctatgctca acgttacgat gtcatcactg cagatcgtga cttggcacct
    25441 gaaattcagg ctatgatcaa acgcacgatt gaacgtgtcg ttgatggtca tgcgcgtgcc
    25501 aaacaagatg aaaaactaga ggcaattttg aactttgcta agtacaactt gcttcctgaa
    25561 gattctatta cgatggaaga cttgtcaggc ttgtctgata aggccatcaa ggaagagctt
    25621 ttccaacgtg ccttgaaggt ttacgatagt caggtttcaa aactacgcga tgaagaagca
    25681 gttaaagaat tccaaaaagt tttgattcta cgagtggtgg ataacaagtg gacagatcat
    25741 atcgatgccc ttgatcaatt gcgtaacgcg gttggacttc gtggctatgc tcagaacaac
    25801 cctgttgttg agtatcaggc agaaggtttc cgtatgttta atgatatgat tggttcgatt
    25861 gagtttgatg tgacacgctt gatgatgaaa gcacaaattc atgaacaaga aagaccacag
    25921 gcagaacgtc atatcagtac aacagcgact cgcaatatcg ctgctcacca agcaagtatg
    25981 ccagaagatt tggatttgag ccagattgga cgcaatgaac tttgcccatg tggttctggt
    26041 aagaaattta aaaactgtca cggtaaaaga caataaaatg agatagttta gaggcggata
    26101 tcttgtgaaa agtaaatttt tactgggtat ccgtttgctt tataaggaga tgagttatgg
    26161 tatttacagc aaaaagctct aaaataaata tagaagaagt tcgtgccttg tcaaaattag
    26221 aaggtcaggc tttggagagg aaatcacagc gcgatcaaga gctagaagcc attatacgtg
    26281 gagaagatca gcgaattctc ttggtaatcg ggccatgctc atctgacaac gaagaagctg
    26341 tccttgaata cgctaagcgt ttggcagtcc tacaagaaga agtggcagat cgtatcttta
    26401 tggttatgcg tgtttatact gccaaacccc gtaccaacgg agatggctat aagggcttga
    26461 ttcaccagcc taacgcgaca gaagcgccta gtcttatcaa tggaatcaaa gccgttcgcc
    26521 atcttcacta tcgtgtcatc acagaaacag ggatgacaac tgctgatgaa atgctttatc
    26581 ctgaaaacct tccgcttgta gatgatttga tttcttacat ggcagttggt gcccgttcag
    26641 ttgaagacca gcaacaccgc tttgtggcaa gtggggcagg attttctact ggttttaaaa
    26701 atccaacctc tggaaatctc aatgtcatgt ttaatgggat ttatgctgct caaaacaaac
    26761 aaagtttcct tttcttagga aaagaagtag aaacaactgg gaacccgctt tcacacgcta
    26821 ttcttcgtgg tgctcttaat gagtatggaa aaaatattcc caactactat tatgacaatt
    26881 taattgatac cattgcccaa tatgagaaaa tgggcttgga aaatcctttt atcatcattg
    26941 ataccaatca tgacaattct ggtaagcagt atattgaaca gatccgaatt gtccgccaga
    27001 ccttgattaa ccgtgcttgg aatgaaaaaa ttaagcagtt cgttcgtggt tttatgattg
    27061 agtcttatct ggaagatggt cgacaaaatg agccagaagt atttggcaag tctatcacag
    27121 acccttgcct gggttgggat aacacagaag ctcttgtcag agaaatttac aaaacgttag
    27181 gagaataaga tggcatttat tgaaaaaggt caagaaatcg atatggaagt catcaaggct
    27241 gaaacccaat tgtctgcgga agccttgaga ctcaaggaaa gccgtgacag ggaattggca
    27301 gatattattt caggggaaga tgaccgtatt ctcttggtga ttggtccttg ctcttctgat
    27361 aatgaagagg cggtcttgga atatgctcgc cgtttatccg ccttgcaaaa gaaggtagcg
    27421 gacaagattt tcatggtcat gcgcgtgtat actgctaagc ctcgtaccaa tggagacggc
    27481 tataaaggat tggttcacca gccagatact tctaaggctc caagcctgat taacggtttg
    27541 caggctgtgc gccagttgca ttaccgcgtg attacagaga ctggtttgac aacggcagat
    27601 gagatgcttt atccgtcaaa tctgatcttg gtggatgact tggtcagcta ccatgccgtt
    27661 ggagctcgtt ctgtggaaga ccaagagcac cgctttgtgg cttctgggat tgatgcacca
    27721 gtagggatga aaaatccaac ctcaggaaat ttgggtgtta tgtttaacgc catctatgct
    27781 gctcaaaaca agcaaacctt cctttatcat gggcaggaag ttgagacatc aggtaatcct
    27841 ttggcccatg ttatcctccg tggagcagtc aacgagtatg gcaattatat gccgaattac
    27901 tactatgaaa acctactcca agccattgaa cgctatgaaa ccatgggact tgaaaatcct
    27961 tttatcctca ttgacaccaa ccatgataac tcaggcaagc aatatatgga gcagattcga
    28021 attgttcgcc agaccttgca gaatcgtgat tggaatgaga aaattaaaaa gacggttcga
    28081 ggatttatga ttgaatctta cctagcagat ggtcgtcaaa accaaccaga gatctttggt
    28141 tgctctatta ctgacccttg cctaggttgg gaaaatacag aggccttggt agaagagatt
    28201 tatgttacct tgacaaaata agtgaaaagg atggagttgg ggaatctcaa ctccttttga
    28261 tgagaatgat agttggacac ggaattgaca tcgaagaatt ggcttcgata gaaagcgcag
    28321 ttacacgaca tgaaggattt gctaagcgtg tactgaccgc tcaggaaatg gagcgcttca
    28381 ccagtctcaa aggacgcagg caaatagaat atttagctgg tcgctggtcg gctaaggagg
    28441 ccttttccaa ggctatggga acgggcatta gcaagctcgg ttttcaggat ttggaagtat
    28501 tgaacaatga acgtggggcg ccttatttta gtcaggcacc attttcagga aagatttggc
    28561 tgtctatcag ccacaccgat cagtttgtga cagccagtgt cattttggag gaaaatcatg
    28621 aaagctagtc cacatagacc aaccaaggct ctgattcatc tgggagctat tcgacaaaat
    28681 attcagcaaa tgggggctca tatccctcaa ggaacgctca agttggctgt ggttaaggcc
    28741 aatgcttatg gtcatggagc tgttgccgtt gccaaggcaa ttcaagatga tgttgatggc
    28801 ttttgcgttt ccaatatcga tgaagccatt gaactcagac aagctggact cagcaagcca
    28861 atcctcattt taggagtttc tgaaatcgaa gctgttgctc tagctaaaga atatgacttc
    28921 accttgacag tggctggact ggagtggatt caagcactct tagataagga agtggaccta
    28981 actggattga cagtccacct caagattgat tcagggatgg gacggattgg ttttagagag
    29041 gcaagtgagg ttgagcaggc tcaagatttg ctccaacaac acggtgttcg tgttgaagga
    29101 atctttaccc actttgctac tgctgatgag gaatcagatg actattttaa tgcccagtta
    29161 gaacggttta aaactatttt agctagtatg aaggaagttc cagagctggt tcatgctagc
    29221 aattctgcaa cgactctttg gcatgtagag actattttca atgcggttcg tatgggagat
    29281 gccatgtatg gtctcaatcc aagtggagcg gtcttggctt tgccttatga tttgataccg
    29341 gccttgacct tggagtctgc tctggttcat atcaagacag ttccagctgg tgcttgcatg
    29401 ggctatggag caacttatca aacggatagc gagcaagtca tcgcgaccgt gccaatcggg
    29461 tatgcagatg gatggacaag agacatgcaa aatttctctg tcttggtaga tggccaagct
    29521 tgcccaattg tcggcagggt ttcgatggac caaatcacta ttcgattgcc taagccttac
    29581 ccactcggaa ccaaggtaac cttgattggc tccaatgggg ataaggaaat cactgcaact
    29641 caggtagcga cctaccgcgt aaccattaac tatgaggtgg tttgcctcct cagcgaccgt
    29701 attccgagag aatattacta gaaaagaaag gagtggagca tgaatctaca tcaacccttg
    29761 catgtcttgc ctggtgtggg accaaagtca gcagaaaaat acgccaaact aggaattgaa
    29821 aacttgcaag atctcttgct ctactttcct ttccgttatg aagacttcaa aatcaagcag
    29881 gtgctggagc tggaagacgg tgagaaggca gttctttctg gtcaggtagt gactcctgct
    29941 agtgtccagt attatggttt caagcgcaat cgcctgcgtt ttagtctcaa gcagggagag
    30001 gtcgtttttg cggtgaattt ctttaaccag ccctatctgg ctgataaaat agagttggga
    30061 gcaacccttg ctgtctttgg aaaatgggac cgcgctaagg ctagtctgac tgggatgaag
    30121 gttctggctc aggtagaaga tgacctccag cctgtctatc gtctggctca gggaatcagt
    30181 caggccagtc tggttaaggt catcaagacg gcttttgatc agggactgga cctcttgata
    30241 gaagaaaatc tgccccagtc tttactagac aaatacaaac tcatgtcccg ttgtcaggca
    30301 gtccgagcta tgcattttcc aaaggatttg gcagaataca agcaggctct tcgccgtatc
    30361 aagtttgagg aactctttta tttccaaatg cagctgcaga tgctcaagtc tgaaaataga
    30421 gttcagggaa gtggtctggt tctgaattgg tctcaggaaa aagtgacagc agttaaagta
    30481 agtcttcctt ttgccctgac ccaagctcag gaaaagagtt tgcaggaaat tttaactgat
    30541 atgaagtccg accaccacat gaatcgtctc ctacaagggg atgtggggag tggaaaaacg
    30601 gtagtcgctg gcttggccat gtttgcggca gtgacagcag gttatcaggc tgccctaatg
    30661 gtaccaacag aaatcctcgc agagcaacac tttgagagtt tacagaacct ttttcccaat
    30721 ttgaaactgg ctctcttgac aggttccttg aaagctgcag aaaagagaga ggtcttggag
    30781 accattgcca agggtgaggc tgatttgatt atcggaactc acgctctgat acaagatggg
    30841 gtggagtatg ctcgtcttgg tttgattatt atcgatgagc agcaccgttt tggtgtaggg
    30901 caaaggcgta ttttacggga aaaaggtgac aatccagatg tcctcatgat gacggcgact
    30961 cccattccac ggacgcttgc catcacagcc tttggagata tggatgtttc cattatcgac
    31021 cagatgccag caggtcggaa gcctattgtg acgcgctgga tcaaacatga gcaactacct
    31081 caggtcttga cttggttaga gggggaaatt caaaaaggtt cccaagccta tgtcatctct
    31141 cctttgattg aagaatcaga agctctagat ttgaaaaatg ccattgcctt atcagaggag
    31201 ttgacgactc attttgcagg caaggcagag gtggctcttc tacatggtag gatgaagagt
    31261 gacgaaaaag accagatcat gcaggatttc aaggagagaa agacggatat tctggtttcg
    31321 acgacggtta ttgaggttgg ggtcaacgtt cccaatgcga ctgtcatgat tatcatggat
    31381 gccgatcgct tcggtctcag tcaacttcac cagcttagag gtcgtgtcgg tcggggggac
    31441 aagcagtcct acgctgttct cgttgccaat cccaagacgg attctgggaa agaccgcatg
    31501 cgtatcatga cagaaacgac caatggattt gtccttgcgg aggaagattt gaaaatgcgt
    31561 ggttctggtg agatttttgg aaccagacag tcaggacttc cagagttcca agtggctgat
    31621 attatcgaag attttccgat tttagaagaa gcaagaaagg ttgctagcta cattagttct
    31681 atagaagctt ggcaagaaga tccagagtgg cgcatgattg cccttcatct ggaaaagaaa
    31741 gaacatctgg attaagcttt ctctaaggaa aacttaagct agtttttagg tttggctctt
    31801 atactaaagt catcaaaaag aaacgaggac tctcatatga cagtaacgat taaagtaaat
    31861 taccaaacca ctttccaaaa gaaggaagca aaaaactagt ataaacagaa gagagagcga
    31921 aatgctcttt ttcgtttcta aaactacttt cagcccatca tcctaaaagt aaagaatcta
    31981 aattcacttt ctatttatcc ttctttcttg cattgattac atagatatgc tacagttgtg
    32041 gtaacgatta caaaataaaa ggagcatgct atgaaaaatc cagctttgct agaagaaatt
    32101 aagacctata gaggaaggga tgaggttccg gaagactttg atgctttctg ggatggggaa
    32161 gtgaaaaatg tttccacgct tccatcctac cacttggagg aaagagattt ccacattcct
    32221 caagtcaagt gctatgagtt aacatttgaa ggaagcaagg aaggaaaggt ctatgcacgc
    32281 attgttcttc caaagagtga ggagaaggtt ccactaatct tccattttca tggttatatg
    32341 ggacgtggct gggactgggc cgacatgctg ggcttcaccg tagctggtta cggtgttgtt
    32401 tccatggatg tgcggggcca gtcaggttac tcacaagacg gcttgcgttc tcctttagga
    32461 aataccgtga aggggcatat tatccgtggt gctatggaag gtcgggacca cctcttttat
    32521 aaggatgttt atctggatat ttaccagttg gtcgaaattg ttgctagtct gtctcaggtt
    32581 gatgagaagc gtctttctag ctatggtgcc tcacaaggag gggctctagc tctagttgca
    32641 gcagcgctca atcctcgaat tcagaaaaca gttgccattt atcccttctt gtcagacttc
    32701 agacgggtga ttgagattgg taatactagc gaggcttacg acgaactttt ccgttatttc
    32761 aagtttcacg accccttcca tgaaacagag gaggaaatca tggcgaccct tgcctatatc
    32821 gatgtcaaaa atcttgccca tcgtatccaa ggtgaggtta agatgattac gggcttggac
    32881 gacgatgttt gctatcccat tacccagttt gcgatttata atcgtctgac ctgcgataaa
    32941 acctatcgca tcatgcctga gtatgctcac gaagccatga atgtatttgt caatgaccaa
    33001 gtctacaact ggctctgtgg aagtgagatt ccttttaaat atctaaaata aggagtcgac
    33061 tctaagcaca aaatcttaaa aattacaaac acgcatagta tcaggggatt aaaaaaactt
    33121 gatactatgc gttttatcat ggaaatatag taaaatgaaa caagaatagg acaaaacgat
    33181 caggacagtc aaatcgattt ctaacaatgt tttagaagca aatgtgtact attctagttt
    33241 caatctatta tatttataga attttttgtt gctagatttg tcaaattgct taaaataatt
    33301 tttttcagaa agcaaaagcc gatacctatc gagtagggta gttcttgcta tcgtcaggct
    33361 tgtctgtagg tgttaatact tttcaaaaat ctctttaaac cacgtcagct tcgccttgcc
    33421 gtatatatgt tactgacttc gtcagttcta tctgccacct caaaacggtg ttttgagctg
    33481 actttgtcag tcttatctac aacctcaaaa cagtgttttg agcatcatgc ggctagcttc
    33541 ttagtttgct ctttgatttt cattgagtat aaaaacagat gagtttctgt tttcttttta
    33601 tggactataa atgttcagct gaaactactt tcaaggacat tattatataa aagaattttt
    33661 tgaaactaaa atctactata ttacactata ttgaaagcgt tttaaaaatg aggtataata
    33721 aatttactaa cgcttataaa aagtgataga atctatcttt atgtatattt aaagatagat
    33781 tgctgtaaaa atagtagtag ctatgcgaaa taacagatag agagaaggga ttgaagctta
    33841 gaaaagggga ataatatgat atttaaggca ttcaagacaa aaaagcagag aaaaagacaa
    33901 gttgaactac ttttgacagt ttttttcgac agttttctga ttgatttatt tcttcactta
    33961 tttgggattg tcccctttaa gctggataag attctgattg tgagcttgat tatatttccc
    34021 attatttcta caagtattta tgcttatgaa aagctatttg aaaaagtgtt cgataaggat
    34081 tgagcaggaa gtatggtata aatagcataa gctgatgtcc atcatttgct tataaagaga
    34141 tattttagtt taattgcagc ggtgtcctgg tagataaact agattggcag gagtctgatt
    34201 ggagaaagga gaggggaaaa ttggcaccaa tttgagatag tttgtttagt tcatttttgt
    34261 catttaaatg aactgtagta aaagaaagtt aataaaagac aaactaagtg cattttctgg
    34321 agtaaatgtc ttatttcaga aatcgggata tagatataga gaggatcagt atgaatcgga
    34381 gtgttcaaga acgtaagtgt cgttatagca ttaggaaact atcggtagga gcggtttcta
    34441 tgattgtagg agcagtggta tttggaacgt ctcctgtttt agctcaagaa ggggcaagtg
    34501 agcaacctct ggcaaatgaa actcaacttt cgggggagag ctcaacccta actgatacag
    34561 aaaagagcca gccttcttca gagactgaac tttctggcaa taagcaagaa caagaaagga
    34621 aagataagca agaagaaaaa attccaagag attactatgc acgagatttg gaaaatgtcg
    34681 aaacagtgat agaaaaagaa gatgttgaaa ccaatgcttc aaatggtcag agagttgatt
    34741 tatcaagtga actagataaa ctaaagaaac ttgaaaacgc aacagttcac atggagttta
    34801 agccagatgc caaggctcca gcattctata atctcttttc tgtgtcaagt gctactaaaa
    34861 aagatgagta cttcactatg gcagtttaca ataatactgc tactctagag gggcgtggtt
    34921 cggatgggaa acagttttac aataattaca acgatgcacc cttaaaagtt aaaccaggtc
    34981 agtggaattc tgtgactttc acagttgaaa aaccgacagc agaattgcca aatggtcgag
    35041 ttcgcctgta tgttaatggt gttttatctc gaacaagtct gaaatctggc aatttcatta
    35101 aagatatgcc agatgtaacg catgtgcaaa tcggagcaac caagcgtgcc aacaatacgg
    35161 tttgggggtc aaatctacag attcggaatc tcactgtgta taatcgtgct ttaacaccag
    35221 aagaggtaca aaaacgtagt caacttttta aacgctcaga tttagaaaaa aaactacctg
    35281 aaggagcggc tttaacagag aaaacggaca tattcgaaag cgggcgtaac ggtaaaccaa
    35341 ataaagatgg aatcaagagt tatcgtattc cagcacttct caagacagat aaaggaactt
    35401 tgatcgcagg tgcagatgaa cgccgtctcc attcgagtga ctggggtgat atcggtatgg
    35461 tcatcagacg tagtgaagat aatggtaaaa cttggggtaa ccgagtaacc attaccaact
    35521 tacgtgacaa tccaaaagct tctgacccat cgatcggttc accagtgaat atcgatatgg
    35581 tgttggttca agatcctgaa accaaacgaa tcttttctat ctatgacatg ttcccagaag
    35641 ggaagggaat ctttggaatg tcttcacaaa aagaagaagc ctacaaaaaa atcgatggaa
    35701 aaacctatca aatcctctat cgtgaaggag aaaagggagc ttataccatt cgagaaaatg
    35761 gtactgtcta tacaccagat ggtaaggcga cagactatcg cgttgttgta gatcctgtta
    35821 aaccagccta tagcgacaag ggtgatctat acaagggtaa ccaattacta ggcaatatct
    35881 acttcacaac aaacaaaact tctccattta gaattgccaa ggatagctat ctatggatgt
    35941 cctacagtga tgacgacggg aagacatggt cagcgcctca agatattact ccgatggtca
    36001 aagccgattg gatgaaattc ttgggtgtag gtcctggaac aggaattgta cttcggaatg
    36061 ggcctcacaa gggacggatt ttgataccgg tttatacgac taataatgta tctcacttaa
    36121 atggctcgca atcttctcgt gtcatctatt cagatgatca tggaaaaact tggcatgctg
    36181 gagaagcggt caacgataac cgtcaggtag acggtcaaaa gatccactct tctacgatga
    36241 acaatagacg tgcgcaaaat acagaatcaa cggtggtaca actaaacaat ggagatgtta
    36301 aactctttat gcgtggtttg actggagatc ttcaggttgc tacaagtaaa gacggaggag
    36361 tgacttggga gaaggatatc aaacgttatc cacaggttaa agatgtctat gttcaaatgt
    36421 ctgctatcca tacgatgcat aatggaaaag aatacattat cctcagcaat gcaggtggac
    36481 cgaatcgtga aaatgggatg gtccacttgg cacgtgtcga agaaaatggt gagttgactt
    36541 ggctcaaaca caatccaatt caaaaaggag agtttgccta taattcgctc caagaattag
    36601 gaaatgggga gtatggcatc ttgtatgaac atactgaaaa aggacaaaat gcctataccc
    36661 tatcatttag aaaatttaat tgggaatttt tgagcaaaga tctgatttct cctaccgaag
    36721 cgaaagtgaa gcgaactaga gagatgggca aaggagagat gggcaaagga gttattggct
    36781 tggagtttga ctcagaagta ttggtcaaca aggctccaac ccttcaattg gcaaatggta
    36841 aaacagcgac tttcctaacc cagtatgata gcaaaacctt gttgtttgca gtagataagg
    36901 aagatatcgg acaggaaatt attggtatag ctaaaggaag catcgaaagt atgcataatc
    36961 ttcctgtaaa tctagcaggt gccagagttc ctggcggagt aaatggtagc aaagcagcgg
    37021 tgcatgaagt tccagaattt acagggggag ttaatggtac agagccagct gttcatgaaa
    37081 tcgcagagta taagggatct gattcgcttg taactcttac tacaaaagaa gattatactt
    37141 acaaagctcc tcttgctcag caggcacttc ctgaaacagg aaacaaggag agtgacctcc
    37201 tagcttcact aggactaaca gctttcttcc ttggtctgtt tacgctaggg aaaaagagag
    37261 aacaataaga gaagaattct aaacatttga ttttgtaaaa atagaaggag atagcaggtt
    37321 ttcaagcctg ctatcttttt ttgatgacat tcaggctgat acgaaatcat aagaggtctg
    37381 aaactacttt cagagtagtc tgttctataa aatatagtag attgaaataa gatgtgaaca
    37441 actctatcag gaaagtcaaa ttaatttata gaaaaatttt agcagtcaag gtgtactgtt
    37501 atagattcaa tatattatgt gactattaac cttctcttct cctaaaattg actttcttgt
    37561 tttcttatct tgtccactcg aaacaagtat tgtaagaatt tgattatttt tgaaagtact
    37621 tttaatatac ttgatatagt taaaaaagat ttgaaactaa attccaaatt agaaaaagac
    37681 ttgaaatact aaaaaaaaaa agtatactct aattgaaaac ggtaacaaaa ctaatttaga
    37741 gaatgaaata tagagtattt ctctcttaaa agtttttggt gaaacgagat gtagaaagga
    37801 gatttagcca aagagtctat tagtgctaga ataatagatt agaattattt tagaaaaacg
    37861 aagtgagcag cttataaatt caagtcccca aatagattca tactagtatc ttttgcaaaa
    37921 aataaagggc gacttccttc atgaatatca atttcatcta taaggaaggt agctaattga
    37981 actaacttat ttattctgtt tgtcgctaga aaaatcagac ctccttgtga agattgagga
    38041 gatactgaat gaaaatcaaa gaagaaacta gcaagctagt agcagattgc ccaaaacacc
    38101 gccttgaggt tgtagataag actgacctat ataatccaag gtgaagcgac tgtggtttga
    38161 agagattttc aaagagtata ggctagagag tagtgttttt atgtccttct agtagaaaat
    38221 gctagacaga agaatgggga acttggatag gaaaaataga ttgagaaagg aggttagaag
    38281 agatgattat tacaaaaatt agccatttag gaacttatgt gggagtaaat ccacattttg
    38341 caacattaat agattttcta gaaaaaacag gactagaaaa tttaacagaa ggttcgattg
    38401 ctatcgatgg taatcgattg tttgggaatt gctttactta tctagcagat ggtcaagcag
    38461 gggctttctt tgaaacacac caaaaatatt tggatattca tttagttttg gaaaacgaag
    38521 aagccatggc tgttacatcg ccggaaaatg taagcgttac ccaagaatat gatgaagaga
    38581 aagatattga attatacaca gggaaagtgg aacagttggt tcagttgaga gctggcgaat
    38641 gcctcatcac ttttccagaa gatttacatc aacccaaggt tcgtataaat gatgaacctg
    38701 tgaaaaaagt tgtctttaaa gttgcgattt cttaatgtag aaagagaaga acgatgaaaa
    38761 aaatgagaaa gtttttatgt ctagctggaa ttgcgctagc ggctgttgcc ttggtagctt
    38821 gttcaggaaa aaaagaagct acaactagta ctgaaccacc aacagaatta tctggtgaga
    38881 ttacaatgtg gcactccttt actcaaggac cccgtttaga aagtattcaa aaatcagcag
    38941 atgctttcat gcaaaagcat ccaaaaacga aaatcaagat tgaaacattt tcttggaatg
    39001 acttctatac taaatggact acaggtttag caaatggaaa tgtgccagat atcagtacag
    39061 ctcttcctaa ccaagtaatg gaaatggtca actcagatgc tttggttccg ctaaatgatt
    39121 ctatcaagcg tattggacaa gataaattta acgaaactgc cttaaatgaa gcaaaaatcg
    39181 gagatgatta ctactctgtt cctctttatt cacatgcaca agtcatgtgg gttagaacag
    39241 atttgttaaa agaacataat attgaggttc ctaaaacttg ggatcaactc tatgaagctt
    39301 ctaaaaaatt gaaagaagct ggagtttatg gcttgtctgt tccgtttgga acaaatgact
    39361 taatggcaac acgtttcttg aacttctacg tacgtagtgg tggaggaagc ctcttaacaa
    39421 aagatcttaa agcagacttg acaagccaac ttgctcaaga tggtattaaa tactgggtta
    39481 aattgtataa agaaatctca cctcaagatt ctttgaactt taatgtcctt caacaagcta
    39541 ccttgttcta tcaaggaaaa acagcatttg actttaactc tggcttccat atcggaggaa
    39601 ttaatgccaa cagtcctcaa ttgattgatt cgattgatgc ttatcctatt ccaaaaatca
    39661 aagagtctga taaagaccaa ggaattgaaa cctcaaacat tccaatggtt gtttggaaaa
    39721 attcaaaaca tccagaagtt gctaaagcat tcttagaagc actttataat gaagaagact
    39781 acgttaaatt ccttgattca actccagtag gtatgttgcc aactattaag gggattagcg
    39841 attctgcagc ctataaagaa aatgaaactc gtaagaagtt taaacatgct gaagaagtaa
    39901 ttactgaagc tgttaaaaaa ggtactgcta ttggttatga aaatggacca agtgtacaag
    39961 ctggtatgtt gactaaccaa cacattattg aacaaatgtt ccaagatatc attacaaatg
    40021 gaacagatcc tatgaaagca gcaaaagaag cagaaaaaca attaaacgat ttatttgagg
    40081 ctgttcagta gatgtaaaag actagaaaat aggtgggcta gtgagctgaa aagctctagc
    40141 ccaatcttgt aaaagaaggg agaaggagaa tggttaaaga acgtaattta actcgctgga
    40201 tatttgtttt gccagctatg attatcgtag gattactctt tgtttatccg tttttctcga
    40261 gtatttttta tagctttacc aataagcatt tgattatgcc taattataaa tttgttggtt
    40321 tggctaacta taaagctgtg ctatcagatc ccaacttctt taatgcgttc tttaattcaa
    40381 ttaagtggac cgttttctca ttagttggtc aagttttagt agggtttgta ttggctttag
    40441 ctcttcacag agtacgccac ttcaagaaat tatataggac cttattgatt gttccttggg
    40501 catttcctac catcgttatt gccttttctt ggcagtggat tctaaacggg gtttatggct
    40561 acttacctaa tctaatcgta aaattaggtt taatggaaca tacacctgca tttttgacag
    40621 atagtacatg ggcattccta tgtttggtgt ttatcaacat ttggtttgga gcaccaatga
    40681 ttatggttaa tgtgctttca gctttgcaaa cagtaccaga agaacaattt gaggctgcta
    40741 agatagatgg tgcttcaagt tggcaggtgt tcaagtttat cgtctttcca catattaaag
    40801 tggttgtagg acttctagtt gttttgagaa ctgtatggat ctttaataac tttgacatta
    40861 tctacctcat tactggtggt ggaccagcca atgctacaac gacgcttcca atttttgctt
    40921 acaacctggg ctggggaact aaattgttgg gtcgtgcttc agcagttaca gtattgctct
    40981 ttatcttctt ggtggcgatt tgctttatct actttgctat catcagtaag tgggaaaagg
    41041 agggtagaaa ataatgaaga agaaatccag tatttattta gatattctct cacatgtact
    41101 tttagttggt gcgaccatcg ttgcaatttt cccattggta tggattatca tatcttctgt
    41161 caaagggaaa ggggaattaa ctcagtatcc aacacgattt tggcctgaac agtttacatt
    41221 agattatttc actcatgtta tcaacgattt gcacttcatt gataacattc gaaacagttt
    41281 aatcattgcc ttggctacaa ccctcattgc gattattatt tctgctatgg cagcctatgg
    41341 tattgttcga ttctttccta aattgggagc aatcatgtcg agactactcg tcattaccta
    41401 cattttccca ccaattttgt tagcaattcc ctattcaatt gccattgcta aagttgggtt
    41461 aacaaatagt ttatttggct tgatgatggt ttatctatct tttagtgttc catatgcagt
    41521 ttggctctta gttggatttt tccaaacagt tccaattgga attgaagaag cggctagaat
    41581 tgatggtgca aataaatttg ttacgtttta taaagttgtg ctaccgattg tagcaccagg
    41641 tattgtagca acagctattt atacatttat caatgcttgg aatgaattcc tgtatgcctt
    41701 gattttgatt aacaatacag gaaagatgac agtagcagta gcccttcgtt cacttaatgg
    41761 ttcagaaata ctagactggg gagatatgat ggcagcgtct gttattgtag ttcttccatc
    41821 aattattttc ttctctatca tccaaaataa gattgcaagt ggattatcag aaggatctgt
    41881 gaagtagacg aaagaaggaa aaaaatgaat aaaagaggtc tttattcaaa actaggaatt
    41941 tctgttgtag gcattagtct tttaatggga gtccccactt tgattcatgc gaatgaatta
    42001 aactatggtc aactgtccat atctcctatt tttcaaggag gctcatatca actgaacaat
    42061 aagagtatag atatcagccc tttgttatta gataaattgt ctggagagag tcagacagta
    42121 gtaatgaaat ttaaagcaga tgaaccaaac tctcttcaag ctttgtttgg cctatctaat
    42181 agtaaagcag gctttaaaaa taattacttt tcaattttca tgagagattc tggtgagata
    42241 ggtgtagaaa taagagacgc ccaaaaggga ataaattatt tattttccag accagcttca
    42301 ttatggggaa aacataaagg acaggcagtt gaaaatacac tagtatttgt atctgattct
    42361 aaagataaaa catacacaat gtatgttaat ggaatagaag tgttctctga aacagttgat
    42421 acatttttgc caatttcaaa tataaatggt atagataagg caacactagg agctgttaat
    42481 cgtgaaggca aggaacatta cctcgcaaaa agaagtattg atgaaatcag tctatttaac
    42541 aaagcaatta gtgatcagga agtttcaaat attcccttgt caaatccatt tcagttaatt
    42601 ttccaatcag gagattctac tcaagctaac tattttagaa taccgacact atatacatta
    42661 agtagtggaa gagttctatc aagtattgat gcacgttatg gtgggactca tgattctaaa
    42721 agtaagatta atattgccac ttcttatagt gatgataatg ggaaaacgtg gagtgagcca
    42781 atttttgcta tgaagtttaa tgactatgag gagcagttag tttactggcc acgagataat
    42841 aaattaaaga atagtcaaat tagtggaagt gcttcattca tagattcatc cattgttgaa
    42901 gataaaaaat ctgggaaaac gatattacta gctgatgtta tgcctgcggg tattggaaat
    42961 aataatgcaa ataaagccga ctcaggtttt aaagaaataa atggtcatta ttatttaaaa
    43021 ctaaagaaga atggagataa cgatttccgt tatacagtta gagaaaatgg tgtcgtttat
    43081 gatgaaacaa ctaataaacc tacaaattat actataaatg ataagtatga agttttggag
    43141 ggaggaaagt ctttaacagt cgaacaatat tcggttgatt ttgatagtgg ctctttaaga
    43201 gaaaggcata atggaaaaca ggttcctatg aatgttttct acaaagattc gttatttaaa
    43261 gtgactccta ctaattatat agcaatgaca actagtcaga atagagaaga gagttgggaa
    43321 caatttaagt tgttgcctcc gttcttagga gaaaaacata atggaactta cttgtgtcct
    43381 ggacaaggtt tagcattaaa atcaagtaac agattgattt ttgcaacata tactagtgga
    43441 gaactaacct atctcatttc tgatgatagt ggtcaaacat ggaagaaatc ctcagcttca
    43501 attccgttta aaaatgcaac agcagaagca caaatggttg aactgagaga tggtgtgatt
    43561 agaacattct ttagaaccac tacaggtaag atagcttata tgactagtag agattctgga
    43621 gaaacatggt cggaagtttc gtatattgat ggaatccaac aaacttcata tggcacacaa
    43681 gtatctgcaa ttaaatactc tcaattaatt gatggaaaag aagcagtcat tttgagtaca
    43741 ccaaattcta gaagtggccg taagggaggt caattagttg tcggtttagt caataaagaa
    43801 gatgatagta ttgattggaa ataccactat gatattgatt tgccttcgta tggttatgcc
    43861 tattctgcga ttacagaatt gccaaatcat cacataggtg tactgtttga aaaatatgat
    43921 tcgtggtcga gaaatgaatt gcatttaagc aatgtagttc agtatataga tttggaaatt
    43981 aatgatttaa caaaataaag gagaaaaaca tggttaaata cggtgttgtt ggagcagggt
    44041 attttggagc tgaattggct cgctacatgc aaaagaatga tggagcagag attactcttc
    44101 tctatgatcc agataatgca gaggcgattg cagaagaatt gggagcaaaa gtagcaagtt
    44161 ccttagatga gttggtttct agcgatgaag tagattgtgt tatcgtcgca actccaaata
    44221 atcttcataa ggaaccggtt attaaggctg cacagcatgg taaaaatgtt ttctgtgaaa
    44281 aaccaattgc gctttcttat caagattgtc gcgagatggt agatgcgtgt aaagaaaaca
    44341 atgtaacctt tatggcagga catattatga atttctttaa tggtgttcat catgcaaaag
    44401 aactcattaa tcaaggagtt atcggagacg ttctatattg tcatacagct cgtaatggtt
    44461 gggaagaaca acaaccgtca gtgtcatgga aaaaaattcg tgaaaaatca ggtggtcact
    44521 tgtatcacca catccatgaa ttggattgcg ttcaattcct tatggggggc atgcctgaaa
    44581 ctgtaaccat gacaggtgga aatgtggccc atgaaggtga acatttcggt gatgaagatg
    44641 atatgatttt tgtcaatatg gaattttcta ataagcgttt tgccttgtta gaatggggtt
    44701 cagcttatcg ttggggtgaa cattatgtct taatccaagg aagcaaaggt gccatccgct
    44761 tagacttatt caactgtaaa ggaactctta agctagatgg gcaagaaagc tatttcttga
    44821 ttcacgaatc gcaagaagaa gatgatgatc ggactcgtat ctatcatagt acagagatgg
    44881 atggagcaat tgcttatggt aaaccaggta aacgtactcc attatggcta tcatctgtca
    44941 ttgataaaga aatgcgctat ctgcatgaga ttatggaagg agctccagta tcagaagaat
    45001 ttgcaaaact tttgacaggt gaagctgccc tagaagcaat tgctactgca gatgcttgta
    45061 cccagtctat gtttgaagat cgcaaagtaa aattgtcaga aattgtaaaa taaattttgg
    45121 tattctccta tttataggtc gacttgctcc tctgaaagta cttttagagg agctgtttga
    45181 ctttgctagt ttttgaaact gaaatctatt atactacaaa ctattgaaag cgttttaatt
    45241 ttaaggtata ataatctcat agaaataaag aaaaggagga aagaggatgc cacagattag
    45301 caaagaagcc ttgattgagc aaatcaaaga tggaatcatc gtttcttgtc aggctcttcc
    45361 tcatgaaccg ctttatacag aagcgggagg ggtgattccc ttgctggtca aagcggctga
    45421 gcaaggtgga gcagtcggta tccgagcaaa cagtgttcgc gatatcaagg aaattaagga
    45481 agtcactaaa cttccaatca ttgggattat caaacgtgat tatccacctc aggaaccctt
    45541 catcacggct actatgaaag aagttgatga attggcagaa ctggacatcg aggtgattgc
    45601 tctggattgt accaagcgtg aacgctacga tggtttggaa attcaagagt tcattcgtca
    45661 ggttaaggag aaatatccta atcagctttt gatggctgat actagtatct tcgaagaagg
    45721 gctagcagct gtagaagcag gaattgactt tgtcggaaca accttatcag gctacacatc
    45781 ctacagtcca aaagtagacg gtccagattt tgaattgatt aagaaactct gtgatgccgg
    45841 tgtagatgtc attgcagaag gaaaaattca tacaccagaa caagccaaac aaatccttga
    45901 atatggagtg cgaggcatcg ttgttggtgg cgccattact agaccaaaag agattacaga
    45961 acgcttcgtt gctagtctta aataagatgt gagggggagt tttatgttta aagttttaca
    46021 aaaagttgga aaagctttta tgttacctat agctatactt cctgcagcag gtctactttt
    46081 ggggattggt ggtgcacttt caaacccaac cacgatagca acttatccaa tactagacaa
    46141 tagtattttt caatcaatat tccaagtaat gagctctgca ggagaggttg tattcagtaa
    46201 tttgtcacta cttctctgtg tgggattatg tattggctta gcgaaacgag ataaaggaac
    46261 cgctgcgtta gcaggagtaa ctggttactt agttatgact gcaacgatca aagctttagt
    46321 aaaacttttt atggcagaag gatctgcaat tgatactgga gttattggag cattagttgt
    46381 cggaatagtt gccgtatatt tgcacaaccg atataacaat attcaattac cttccgcttt
    46441 aggattcttt ggaggttcac gcttcgttcc tattgttaca tcgttctctt ctatcttgat
    46501 tggctttgtc ttctttgtta tttggccacc tttccaacaa cttcttgttt ctacaggtgg
    46561 atatatttct caggcgggtc caattggaac ttttctatat ggatttttaa tgagactttc
    46621 tggagcagta ggcttacatc atataattta ccctatgttt tggtatactg aacttggtgg
    46681 tgttgaaact gttgcaggac aaacagtggt tggagctcaa aaaatatttt ttgctcaatt
    46741 agccgatttg gcccattctg gattatttac agaaggaaca aggttttttg caggtcgttt
    46801 ctcaacaatg atgttcggtt taccggctgc ctgtttagcg atgtaccata gtgttcctaa
    46861 aaatcgtcgt aaaaaatacg cgggtttgtt tttgggagtt gctttaacat cttttattac
    46921 cggtattaca gaaccaattg aatttatgtt tctattcgtt agtccggttc tatatgttgt
    46981 tcacgcattc cttgatggtg ttagcttctt tattgcagac gtcttaaata tttcaatagg
    47041 aaacacattt tcaggaggtg taatcgattt cactttattt ggaattttgc aggggaacgc
    47101 taagacgaat tgggttcttc agattccatt tggacttatt tggagtgttt tgtattatat
    47161 tatttttaga tggttcatta ctcaattcaa cgttctaacg ccagggcgag gagaagaagt
    47221 agattctaaa gaaatttctg aatccgcaga ttcaacttca aatactgcag attatttaaa
    47281 acaggatagc ctacaaatta tcagagcctt gggtggatca aataatatag aagatgtaga
    47341 tgcttgtgtg acacgtttac gtgtagctgt aaaagaagtt aatcaagttg ataaagcact
    47401 tttaaaacaa attggtgcag ttgatgtctt agaagtgaag ggtggcattc aagcaatata
    47461 tggagcaaaa gcaatcttat ataaaaatag tattaatgaa attttaggtg tagatgatta
    47521 agtacttact gacttaataa aaaacagagg agagtgatgg atgagtagga tgaaatgaaa
    47581 tcgcatacaa gaaataaaga actcattatc caagttggat acgcttatta cataggagaa
    47641 tacaaatgaa atttagaaaa ttagcttgta cagtacttgc gggtgctgcg gttcttggtc
    47701 ttgctgcttg tggcaattct ggcggaagta aagatgctgc caaatcaggt ggtgacggtg
    47761 ccaaaacaga aatcacttgg tgggcattcc cagtatttac ccaagaaaaa actggtgacg
    47821 gtgttggaac ttatgaaaaa tcaatcatcg aagcgtttga aaaagcaaac ccagatataa
    47881 aagtgaaatt ggaaaccatc gacttcaagt caggtcctga aaaaatcaca acagccatcg
    47941 aagcaggaac agctccagac gtactctttg atgcaccagg acgtatcatc caatacggta
    48001 aaaacggtaa attggctgag ttgaacgacc tcttcacaga tgaatttgtt aaagatgtca
    48061 acaatgaaaa catcgtacaa gcaagtaaag ctggagacaa ggcttatatg tatccgatta
    48121 gttctgcccc attctacatg gcaatgaaca agaaaatgtt agaagatgct ggagtagcaa
    48181 accttgtaaa agaaggttgg acaactgatg attttgaaaa agtattgaaa gcacttaaag
    48241 acaagggtta cacaccaggt tcattgttca gttctggtca agggggagac caaggaacac
    48301 gtgcctttat ctctaacctt tatagcggtt ctgtaacaga tgaaaaagtt agcaaatata
    48361 caactgatga tcctaaattc gtcaaaggtc ttgaaaaagc aactagctgg attaaagaca
    48421 atttgatcaa taatggttca caatttgacg gtggggcaga tatccaaaac tttgccaacg
    48481 gtcaaacatc ttacacaatc ctttgggcac cagctcaaaa tggtatccaa gctaaacttt
    48541 tagaagcaag taaggtagaa gtggtagaag taccattccc atcagacgaa ggtaagccag
    48601 ctcttgaata ccttgtaaac ggatttgcag tattcaacaa taaagacgac aagaaagtcg
    48661 ctgcatctaa gaaattcatc cagtttatcg cagatgacaa agagtgggga cctaaagacg
    48721 tagttcgtac aggtgctttc ccagtccgta cttcatttgg aaaactttat gaagacaaac
    48781 gcatggaaac aatcagcggc tggactcaat actactcacc atactacaac actattgatg
    48841 gttttgctga aatgagaaca ctttggttcc caatgttgca atctgtatca aatggtgacg
    48901 aaaaaccagc ggatgctttg aaagccttca ctgaaaaagc aaacgaaaca atcaaaaaag
    48961 ctatgaaaca atagtcctta gttattctat aaaaagtagt tttttaaaga acctaagagt
    49021 gtataccccc ttttccctct acacagatag tgtaagaaaa gggggctttt gtttaaaatg
    49081 taagaaactg tcacgaaatt aaaatgaagt tcttacataa gcgaatcata aaaaatttca
    49141 ttttgatttt aaaacagttc aagaaagtca aaaaattatt ctatttgaaa gagaggtgcc
    49201 gactgtgaaa gtcaataaaa tccgtatgcg ggaaacagtg atttcctacg ctttcctagc
    49261 accagtatta ttcttctttg tcatctttgt gttggctccg atggtgatgg gcttcattac
    49321 aagtttcttt aactactcaa tgactaaatt tgagtttgta ggcttggata actatatccg
    49381 tatgtttaaa gatcctgtct ttacaaaatc tctgattaac acagttattt tggttattgg
    49441 atctgtacca gttgttgttc tattctcact ctttgtagca tctcagacct atcatcaaaa
    49501 tgtcattgcc agatccttct accgtttcgt cttcttcctt cctgttgtaa cgggtagtgt
    49561 tgccgtgaca gttgtttgga aatggattta tgacccacta tcagggattc taaactttgt
    49621 ccttaagtcc agccacatca tcagccaaaa catttcttgg ttgggagata aaaactgggc
    49681 attgatggcg attatgatta ttctcttgac cacttcagtt ggtcagccca tcatccttta
    49741 tatcgctgcc atggggaata ttgacaattc actggttgaa gcggcgcgtg ttgatggtgc
    49801 aactgagttt caagtttttt ggaagattaa atggccaagc cttcttccaa caactcttta
    49861 tattgcaatc atcacaacaa ttaactcatt ccagtgtttc gccttgattc agcttttgac
    49921 atctggtggt ccaaactact caacaagtac cttgatgtac tacctttacg aaaaagcctt
    49981 ccaattgaca gaatacggct atgccaacac aattggtgtc ttcttggcag tcatgattgc
    50041 tatcgtaagc tttgttcaat ttaaagtact tggaaacgac gtagaatact aaagaaagga
    50101 gacagctatg caatctacag aaaaaaaacc attaacagcc tttactgtta tttcaacaat
    50161 cattttgctc ttgttgactg tgctgttcat ctttccattc tactggattt tgacaggggc
    50221 attcaaatca caacctgata caattgttat tcctcctcag tggttcccta aaatgccaac
    50281 catggaaaac ttccaacaac tcatggtgca gaaccctgcc ttgcaatgga tgtggaactc
    50341 agtatttatc tcattggtaa ccatgttctt agtttgtgca acctcatctc tagcaggtta
    50401 tgtattggct aaaaaacgtt tctatggtca acgcattcta tttgctatct ttatcgctgc
    50461 tatggcgctt ccaaaacaag ttgtccttgt accattggta cgtatcgtca acttcatggg
    50521 aatccacgat actctctggg cagttatctt gcctttgatt ggatggccat tcggtgtctt
    50581 cctcatgaaa caatttagtg aaaatattcc tacagagttg cttgaatcag ctaaaatcga
    50641 cggttgtggt gagattcgta ccttctggag tgtagccttc ccgattgtga aaccagggtt
    50701 tgcagccctt gcaatcttta ccttcatcaa tacttggaat gactacttca tgcagttggt
    50761 aatgttgact tcacgtaaca atttgaccat ctcacttggg gttgcgacca tgcaggctga
    50821 aatggcaacc aactatggtt tgattatggc aggagctgcc cttgctgctg ttccaatcgt
    50881 cacagtcttc cttgtcttcc aaaaatcctt cacacagggt attactatgg gagcggtcaa
    50941 aggataatac tctgcgaaaa tcgaatgcaa actacgtcag cttcaccttg ccatacttaa
    51001 gtattgcctg cggttagctt cctagtttgt tcttcaattt tcattgagta taggaaaatc
    51061 aatctatcaa gatacagaag tatattttat agatttagag aatatagaag ttataagtgt
    51121 ctacaaaatg gagggtatgc agttacttta tgaagttttg tcagacactt atcaacttaa
    51181 gaatgatttt agttaattat cagaaacgaa ggaaagagta tgatttttga cgatttgaaa
    51241 aacatcacct tttacaaagg gattcatccc aatttagaca aagctatcga ctacctctac
    51301 caacatcgta aagattcatt cgaattagga aagtatgaga ttgatggaga taaggtcttt
    51361 ctagttgttc aggaaaatgt cctcaatcaa gttgagaata atcaatttga acaccataag
    51421 aactatgcag atttgcattt gctgatagaa gggcatgaat attcgagcta cggttcacgt
    51481 atcaaagacg aggcagtagc attcgacgaa gcgactgaca ttggctttgt tcattgtcat
    51541 gaacactacc cactcttgtt gggttatcat aattttgcga ttttcttccc aggtgagcca
    51601 catcagccaa ctggctatgc aggtatggaa gaaaaggttc gaaaatatct ctttaaaatt
    51661 ttgattgatt aaaaatagga tgaattgttt ttttgtaaag ctttgacaat actctaccat
    51721 gaaattgatc tttatgaggt agagaaatga gaataaaata tttaaaaatt ggtatcttct
    51781 aagcatgctg caagagctag tttcttagat ggacaggaaa ttacagttga tgagatgtcc
    51841 tggataatta atggcattgt gaatgcattg attggtagat acataaaatt aggtacttat
    51901 gcagctaagt atggtattgg tatagcacgc tcgatcttaa gtagggcagc tgcaactgca
    51961 gcagcaagag taggattact gaccaagatt tctggatgga ttttacgagt agctgtgaat
    52021 gtagctgatg tatatggtaa tttcgccaac aatattgctg cagcttggga tgcatatgat
    52081 aaaattccta acaatggtcg tataaacttt taaaatacga gaatgaaagc actttgtatt
    52141 tttttattga atatgttagc ttggacagtg cttgcaatga taattcgtgg agggctagat
    52201 ggatttgata ggcatacttg gagtactatt ttaattgctt cgctgttcgg ggtatatgat
    52261 tataagccca tagataaaaa tagaaaaaag tccaaaagaa aaaagaaaaa atagatttgt
    52321 tcatggtagg gacttatgaa agctttactg acaaaaaaga aaacagttta caaagaaaaa
    52381 tgatggagga gcaaacatgg cacaaaaagg agtaagcctt atcaaggcag catttgatac
    52441 agataacttt ctcatgcgtt ttagtgagaa ggtcttggac atcgtgacag ccaatcttct
    52501 ttttgtcgtc tcttgtttac ccatcgtgac gattggagtg gctaaaatca gcctctacga
    52561 gaccatgttc gaagttaaga agagcagacg ggtgcctgtt tttaaaatct atctaagatc
    52621 tttcaagcaa aatctgaaac taggtcttca gctgggttta atggagttag gaattgtgtt
    52681 tcttaccctt tcagatctct atcttttctg gggtcaaaca gctctgccct tccaattgct
    52741 gaaagccatt tgtttaggta ttctgatttt tcttactatc gtgatgctgg ctagttaccc
    52801 tatcgcggca cgttatgacc tatcttggaa agaaattctt caaaaaggat tgatgttggc
    52861 tagttttaac tttccttggt tcttcctcat gttagccatt cttgtcctca ttgtgatggt
    52921 tctttatctg tccgccttca gtctactctt aggtggctca gtcttcctac tttttgggtt
    52981 tggactattg gtctttatcc agactggatt gatggagaaa attttcgcaa aataccaata
    53041 ggagctttat ttctgaaact actttcaaag gctccaaacg ctattctata agcgagaaac
    53101 taaaatctaa gtgtctataa gatattgaaa gccttttcgg taaagtgtat actaaagaag
    53161 taaagaaaaa tctgctttag cagaaaataa aaataatagg agaaaatcta tgtcagattt
    53221 gaaaaaatac gaaggtgtca ttccagcctt ctacgcatgt tatgatgagc aaggagaagt
    53281 aagtccagag cgtacgcgtg ccttggttca atacttcatt gataaaggtg ttcaaggtct
    53341 ttatgtcaat ggttcttctg gtgaatgtat ctaccaaagc gttgaagatc gcaagttgat
    53401 tttggaagaa gtcatggcgg tagctaaagg taaattgacc attattgccc atgttgcctg
    53461 caacaatact aaagacagta tggaacttgc tcgccatgct gaaagcttgg gagtagatgc
    53521 tattgcaacg attccaccaa tttatttccg cttgccagaa tactcagttg ccaaatactg
    53581 gaacgatatc agttctgcag ctccaaacac agactacgtg atttacaaca ttcctcaatt
    53641 ggcaggggtt gctttgactc caagccttta cacagaaatg ttgaaaaatc ctcgtgttat
    53701 cggtgtgaag aactcttcta tgccagttca agatatccaa acctttgtca gccttggtgg
    53761 ggaagaccat atcgtcttta acggtccaga tgaacagttc ctaggtggtc gcctcatggg
    53821 tgccaaagct ggtatcggtg gtacttatgg tgctatgcca gaactcttct tgaaactcaa
    53881 ccaattgatt gcggataaag atttagaaac agcgcgtgag ttacagtatg ctatcaacgc
    53941 tatcattggt aaattgactg ctacacatgg aaatatgtac ggtgtcatca aagaagtttt
    54001 gaagatcaat gaaggcttga atattggttc tgttcgttca ccattgacac cagtgactga
    54061 agaagatcgt ccagttgtag aagcggctgc tgccttgatt cgtgaaacca aggagagctt
    54121 cctctaatct aaaaggaggt atttatgaca tactacgttg caattgatat cggtggaacc
    54181 aacatcaagt atggtttggt tgatcaagag gggcaacttc ttgaatcgca tgaaatgcca
    54241 actgaggcgc ataagggtgg acctcatatc ttacaaaaga ccaaagatat cgtagctagt
    54301 tatttagaaa aaggcccagt agcaggtgtt gccatatctt ctgctgggat ggtggatccg
    54361 gataagggtg agattttcta tgctgggccg caaatcccta actacgcagg cacccagttc
    54421 aaaaaggaaa tcgaagaaag ctttactatt ccttgtgaga ttgaaaatga tgtcaactgt
    54481 gcaggtcttg ctgaggcagt atctggttca ggcaagggag caagtgtgac actttgcttg
    54541 accattggaa ccggtatcgg tggttgcttg attatggata ggaaagtctt ccatggtttt
    54601 agcaattcag cctgtgaagt cgggtatatg catatgcagg atggagcttt tcaagacttg
    54661 gcttctacaa cagctttagt gaaatatgta gctgaagccc atggagaaga tgttgatcag
    54721 tggaatggcc gtagaatttt caaagaagcc actgaaggaa acaaaatctg catggaaggt
    54781 attgaccgta tggttgacta tctaggaaaa ggtctggcaa atatttgcta cgttgccaat
    54841 ccagaagtgg ttattcttgg tggtggtatc atggggcaag aggctatcct caaacctaag
    54901 atccgcacag ccttgaaaga ggctttggta ccaagtttag cagaaaaaac acgattagaa
    54961 tttgcccatc accaaaatac agcagggatg ttgggtgcat attatcattt taagacaaaa
    55021 caatcctagt ttggctcagc caaactagga ttttcttaca cgtttttgtc tacgatagcc
    55081 gttgagtttt ttattttccc agtagctatt aaagattttt tccttgcttt cgcgattgat
    55141 ttccaaaaag taggcataaa tcaaatcgat aaagaagagc ataggaagtt gagcggatat
    55201 tcgttggata taggagggtt ggctgtgggt ggctacaaga acagtctctg tataggtctg
    55261 gctatcttta ttgggaacac ttgtaaagag tacagtcttt gcccccatct ccttggcatc
    55321 taatagacta tctaaaatag aaggagttga gcctgaaagt gagaagccca gtactagaca
    55381 attttcatcc atgatgctgg ttgtccaggc aaagccgtct tggtctgtca aagcttcgca
    55441 gaccacacct agtcgcataa aacgtaattt catttcacgg gcgacgaggc cagaactccc
    55501 tgttccaaag aagtagatac gctcagcatc ttcgattagc tgggcaattc gctctagttg
    55561 gatttcgtca atcaagtctt gtgtttgttc cctcatattg ctataacttc tgaggactcg
    55621 tttggtcagt ggactgtgct tggagactcg gttggcttga ttttctgcct gatgttggta
    55681 ttggaaaata aattctcggt agccagtaaa gccacacttt ttagcaaagc gggtcaaagc
    55741 agcttgagaa atatgtaatt tttgggtgac ttgttgagaa gataaatcat ctgtaatcgt
    55801 ttcagcttgc aaaaaatagc gagcgatttc ttgctctagg tctgtcattt cttcaaaatg
    55861 tgaatcaatg atagttgcga tatctggttt gtccataggg aaagctcctt tacatgagtc
    55921 atactggaag actagatcag ggaatagtca cacttcatta taacacataa tataaggata
    55981 gataaataaa aacgcatctc tgttttaaaa acgaaaaaat cgaaaaagct tctctctttt
    56041 ccataatttt ctactcaaat tgtggtacaa ttaagagtaa gattttaagt tagaaatgag
    56101 actgatttgt atgagaaaat ttaacagcca ttcgattccg attcggctta atttattgtt
    56161 ttcaatcgtc attttactct ttatgaccat tattggtcgt ttgttgtata tgcaggtttt
    56221 gaacaaggat ttttacgaaa aaaagctagc ctcagctagt cagaccaaga ttacaagcag
    56281 ttcagcccgt ggggaaattt atgatgctag tggaaaacct ttggtagaaa atacgttaaa
    56341 gcaggttgtt tcctttacgc gtagcaataa aatgacggct acagacttaa aagaaacagc
    56401 taaaaagtta ctgacttatg tgagcatcag ttctccaaat ttgacaaaac gccagctggc
    56461 ggattactat ttggctgatc ctgaaatcta taaaaaaaca gtggaagctc tcccaagcga
    56521 gaaacgcttg gattcagatg gcaatcgtct atccgaatca gaactgtata acaatgcggt
    56581 cgatagtgtc caaacaagtc aactaaacta tacagaggat gaaaagaaag aaatctatct
    56641 ttttagtcag ttaaatgctg ttggaaactt tgcgacagga accattgcta cagatcctct
    56701 aaatgattct caggtggctg ttattgcctc tatttcaaag gagatgcctg gcattagtat
    56761 ttctacttct tgggatagaa aggttttgga aacttccctt tcttctatag ttgggagtgt
    56821 atccagtgaa aaagctggtc tcccagcgga agaagcagaa gcctatctta aaaaaggcta
    56881 ttctctaaat gaccgtgtag gaacctccta tttggaaaag caatatgaag agaccttaca
    56941 aggaaaacga tcggtaaaag aaatccatct ggataaatat ggcaatatgg aaagcgtgga
    57001 tacaattgag gaaggtagta agggaaacaa tatcaaactg accattgatt tggccttcca
    57061 agatagcgtg gatgctttgc tgaaaagtta tttcaattct gagctagaaa atggtggagc
    57121 caagtattct gaaggtgtct atgcagtcgc ccttaaccca aaaacaggtg cggttttgtc
    57181 tatgtcaggg attaaacatg acttgaaaac gggagagttg acgcctgatt ccttgggaac
    57241 ggtaaccaat gtctttgttc caggttcggt tgtcaaggcg gcgaccatca gctcaggttg
    57301 ggaaaatgga gtcttgtcag gaaaccagac cttgacagac cagtccattg tcttccaagg
    57361 ttcagctccc atcaattctt ggtatactca ggcttacggt tcattcccta tcacagcggt
    57421 ccaagctctg gagtattcat caaataccta tatggtccaa acagccttag gtcttatggg
    57481 gcaaacctat caacccaata tgtttgtcgg caccagcaat ctagagtctg ctatggagaa
    57541 actgcgttca acctttggcg aatatggctt gggtactgcg acaggaattg acctaccaga
    57601 tgaatctact ggatttgttc ccaaagagta tagctttgct aattacatta ctaatgcctt
    57661 tgggcagttt gataactata cgccgatgca gttggctcag tatgtagcaa ctattgcaaa
    57721 taatggtgtt cgtgtggctc ctcgtattgt tgaaggcatt tatggtaata atgataaggg
    57781 aggactgggt gacttgattc agcaactgca accgacagag ataaataagg tcaatatatc
    57841 cgactccgat atgagcatct tgcaccaagg tttttatcag gttgcccatg gtactagtgg
    57901 attgacaact ggacgtgcct tttcaaatgg tgccttggta tccattagcg gaaaaacagg
    57961 tacagccgaa agctatgtgg cagatggtca gcaagcaacc aataccaatg cggtggccta
    58021 tgccccatct gataatcccc aaatcgctgt cgcagtggtc tttcctcata ataccaatct
    58081 aacaaatggt gtaggacctt ccattgcgcg tgacattatc aatctgtatc aaaaatacca
    58141 tccaatgaac tagaaaggaa attatgcttt atccaacacc tattgccaag ttgattgaca
    58201 gttattctaa gttaccaggt atcgggatta agacggctac gcgtctggcc ttttatacga
    58261 ttgggatgtc tgctgatgat gtcaatgaat ttgcaaaaaa tctcctttct gctaagagag
    58321 aattgacata ttgttctatt tgtggacgtt tgacagacga cgatccttgt tctatctgta
    58381 ctgatccgac tcgtgaccag acaacaattt tagttcttga ggatagtaga gatgtggcag
    58441 ccatggaaaa tatccaagaa taccatggac tctatcatgt cctgcatggc ctcatttctc
    58501 ctatgaatgg tatcagtccg gacgatatca atctcaagag ccttatgact cgtcttatgg
    58561 atagtgaggt ttcagaagtg attgtggcga ctaatgctac agcggatggt gaagcgactt
    58621 ccatgtatct ttcacgtttg ctcaagccag ctggtatcaa ggttacgcgt ctagcacgag
    58681 gtctcgctgt gggagcggac attgagtatg cggacgaagt gacactctta cgagcgattg
    58741 aaaatcggac agagttgtaa gtgtaggcaa atttacgaac tcccttcatt tataaaaaat
    58801 caaagaggct gaaaatcgtt cctatcggcc tctttttgta tagtgtgatg agtaggatcg
    58861 ggttcaagtt ttaaaaaacc aagcaaatat gatatactaa agagcgagta ttctagtaga
    58921 attaggacaa ataatatgaa acaaacgatt attcttttat atggtggacg gagtgcggaa
    58981 cgcgaagtct ctgtcctttc agctgagagt gtcatgcgtg cggtcaatta cgaccgtttc
    59041 acagtcaaga ctttctttat cagtcagtca ggtgacttta tcaaaacaca ggaatttagt
    59101 catgctccgg ggcaagaaga ccgtctcatg accaatgaaa ccattgattg ggataagaaa
    59161 gttgcaccaa gtgctatcta cgaagaaggt gcagtggtct ttccagtcct tcacgggcca
    59221 atgggagaag atggctctgt tcaaggattc ttggaagttt tgaaaatgcc ttacgttggt
    59281 tgcaacattt tgtcatcaag tcttgccatg gataaaatca cgactaagcg tgttctggaa
    59341 tctgctggta ttgcccaagt tccttatgtg gctatcgttg aaggcgatga tgtgactgct
    59401 aaaatcgctg aagtggaaga aaaattggct tatccagtct tcactaagcc gtcaaacatg
    59461 gggtctagtg tcggtatttc taagtctgaa aaccaagaag aactccgtca agccttaaaa
    59521 cttgccttcc gatatgacag ccgtgtcttg gttgagcaag gagtgaatgc ccgtgaaatt
    59581 gaggttggcc tcttgggtaa ctacgatgtc aagagcacgc tacctggaga agttgtcaag
    59641 gacgttgcct tttatgacta cgatgccaag tatattgata acaagattac tatggatatt
    59701 cctgccaaaa tcagtgatga tgtggtagct gtcatgcgtc aaaatgcaga aacagccttc
    59761 cgtgccattg gtggccttgg tctatctcgt tgcgatttct tctatacaga taagggagag
    59821 atttttctca acgagctcaa taccatgcca ggtttcaccc agtggtctat gtacccacta
    59881 ctttgggaca atatggggat cagctaccca gaactaatcg agcgtttggt tgaccttgcc
    59941 aaggaaagtt ttgacaagcg cgaagcgcat ttgatataaa atgaaagaga gggtagaagc
    60001 cagaaccatc actgcaaggt gactagagtt ctcggacttc agcccttttt aaaggagtag
    60061 aaatgaaatt aacaatccat gaagttgccc aagttgttgg agccaaaaat gatatcagta
    60121 tctttgagga cacccagtta gaaaaagctg agtttgatag tcgtttgatt ggaactggag
    60181 atttatttgt gcccctaaaa ggtgtgcgtg acggtcatga ctttatcgaa agagcttttg
    60241 aaaatggtgc ggcagcaacc ctgtctgaga aagaggttgc aaatcatccc tacattctag
    60301 tagatgatgt tttgactgcc tttcaacaac tagctgccta ctatcttgaa aaaacagctg
    60361 ttgatgtctt tgcggttacg ggttcaaatg gcaagacaac gactaaggat atgttggcgc
    60421 atttactgtc aacaagatac aagacctaca aaacacaagg caattacaat aatgagattg
    60481 gccttcctta cacagttctc cacatgcctg aaggaacaga aaagttggtc ttggagatgg
    60541 ggcaggacca cttgggagat attcatctct tgtctgaatt ggctcgtcca aaaacagcca
    60601 tcgtgacctt ggttggagaa gctcatttgg cctttttcaa agatcgttct gagattgcta
    60661 aaggaaaaat gcaaattgca gacggaatgg cttcaggttc cttgctttta gcgccggctg
    60721 accctatcgt agaggactat ttgccaactg ataaaaaggt ggttcgtttt ggtcaaggag
    60781 cagagctgga aattactgac ttggttgagc gcaaagatag tctgaccttc aaggccaatt
    60841 tcttagagca agtccttgat ttgccagtaa ctggcaagta caatgcgaca aatgctatga
    60901 ttgcatccta tgttgccttg caagaaggag tttcagagga gcaaattcgt ttggccttcc
    60961 aacatcttga attgacgcgt aaccgtaccg agtggaagaa agcagccaat ggagcagaca
    61021 tcttgtcaga tgtttacaat gccaatccaa ctgctatgaa gctgatttta gagaccttct
    61081 ctgccattcc agccaatgaa ggtggcaaga aaattgcagt gttggcggat atgaaggaac
    61141 ttggtgtcca gtctgttcaa cttcataatc agatgatttt gagcctttcg ccagatgtgc
    61201 ttgatactgt tattttctac ggagaagata ttgctgaatt agcccaactt gctagtcaaa
    61261 tgttcccaat cggacacgtt tattacttca agaaaacaga agaccaagac caatttgaag
    61321 acctagttaa gcaggtcaaa gaaagccttg gggctcatga ccaaatcctg ctcaaaggct
    61381 ctaactctat gaatctagcc aagttggtag aaagtttaga aaatgaagac aagtgatttt
    61441 gtcaagtatt tgcaaagaat gattgccatt acagatactg gattaacctt tacaaaagat
    61501 ccgtttgacc gtgagcgcta cgaagacttg cgagaccttc tatctgaaat gttgaatcaa
    61561 gcatcagacc ttgattccga agaagtggca gaagtcttga agccaacttc tgcttatgcg
    61621 actcccttaa tggacgtccg tgcttggatt gttgaggacg aaaagatttg tctggttagg
    61681 ggacaaggag aggatagttg ggctttgcca ggtggctttg gtgaagtcgg ctattctcca
    61741 actgaaaata ttctcaagga aattgaagaa gaaaccggtt ttaaagccaa agttgaaaga
    61801 ctgctagctg tttttgatac caatcgtttc caactacaga gcaaacaata tgcaaagttt
    61861 gtttttgaat gtaagcttct cgatggacaa ttccaagaaa atcaagaaat cgctgacctt
    61921 caattttttg caattgaccc actgccgaat ttatctgaaa aacgcattac caaggagcaa
    61981 atcgagattc tttggcaggt ttatcaaggt cagagggagc aatatcttga ctaagaagat
    62041 gattatcgta tttctaaatt catttttctt aagtagcatg gtataataaa atgcaggaaa
    62101 attttgaatc atgaggaaga ctagatgaat ttatgggata ttttctttac gactcaggca
    62161 accgagccgc ccaaatttga ccttttttgg tatgttagcc tatttacgct cttagcctta
    62221 accttttata cagcccatcg ctatcgtgaa aagaaggttt accaacgatt tttccaaatc
    62281 ttgcagactg ttcagttaat ccttctttat ggttggtact gggtcaatca tatgccactg
    62341 tcagaaagcc taccctttta ccattgccgt atggctatgt ttgtggtact cttgcttcct
    62401 ggtcagtcca aatataaaca atactttgca ttattgggaa catttgggac attagcagcc
    62461 tttgtttatc cagtgccaga tgcttaccct tttccacata tcaccattct atcctttatc
    62521 tttggtcatt tagcactctt ggggaactct ctagtttatc tattgagaca gtataatgcg
    62581 cgattgctgg atgtgaaggg aatttttctc atgacctttg ccctaaatgc cttgattttt
    62641 gtggtcaatt tggtgacagg tggcgattac ggatttttga caaaaccgcc attggttggg
    62701 gatcacggtc tagtagctaa ttatttactt gtttcaattg tgctggtagc tactatcagt
    62761 ttgactaaga aaatcttaga attcttttta gctcaagaag cagaaaaaat gattgcaaag
    62821 gaagcttaac acagagcttt cttttttgct cttagagagt ttttacaagc agcttataaa
    62881 ataagaattt ctgaatagac aaactcaaaa aatggctggg aaatttagga aaaaagcaag
    62941 cacgattaaa ttttttgtgt tataatattt tgtgaatagc tatgcctatg tttagctatg
    63001 gaataatacg aagtgcgaaa cttggaagat agagaggaag cgatgtaatg gctagagaag
    63061 gcttttttac aggtctagat attggaacaa gctctgtcaa ggtgcttgtg gccgagcaga
    63121 gaaatggtga attaaatgta attggcgtga gtaatgccaa aagtaaaggt gtaaaggatg
    63181 gaattattgt tgatattgat gcagcagcaa ctgctatcaa gtcagccatt tcccaagcgg
    63241 aagaaaaggc aggcatttcg attaaatcag taaatgtcgg cttgcctggt aatcttttgc
    63301 aggtagaacc aactcagggg atgattccag taacatctga tactaaggaa attacggatc
    63361 aagatgttga aaatgttgtc aaatcagctt tgacaaagag tatgacacct gaccgtgaag
    63421 tcattacctt tattcctgaa gaatttattg tggatggttt ccaagggatt cgtgacccac
    63481 gtggcatgat gggggttcgc cttgaaatgc gtggtttgct ttatacagga cctcgtacta
    63541 tcttgcacaa tttgcgtaag acggttgagc gtgcaggtgt tcaggttgaa aatattatca
    63601 tttcaccact agcaatggtt cagtctgttt tgaatgaagg ggaacgtgaa tttggtgcta
    63661 cagtgattga tatgggggca ggtcaaacga ctgtcgctac aatccgtaat caagaactcc
    63721 agttcacaca tattctccaa gaaggtggag attatgtaac taaagatatc tccaaggttt
    63781 tgaaaacctc tcgcaaatta gcggaaggct tgaaactgaa ttacggggaa gcctatccgc
    63841 ctcttgcaag caaagaaacc ttccaagtag aggttattgg agaagtggaa gcagtcgaag
    63901 tgacggaagc ctacttgtca gaaattattt ctgcacgaat caagcacatc cttgaacaaa
    63961 tcaagcaaga attagataga aggcgtctat tggacctccc tggtggtatt gtcttaatcg
    64021 gtgggaatgc cattttacca ggtatggttg agcttgctca ggaagtcttt ggcgtccgtg
    64081 tcaagcttta tgttccaaat caagttggta tccgtaatcc agcctttgcg catgtgatta
    64141 gtttatcaga atttgcgggt caattaacag aagttaatct tttggctcag ggagcgataa
    64201 aaggtgagaa tgacttaagt catcagccaa ttagttttgg tgggatgctg caaaaaacag
    64261 ctcagtttgt acaatcaacg cctgttcaac cagctcctgc tccagaagta gagtcggtgg
    64321 cgcctacaga accaatggcg gatttccaac aagcttcaca aaataaaccg aaattagcag
    64381 atcgtttccg tggcttgatc ggaagcatgt ttgacgaata aagaggaaaa ataaattatg
    64441 acattttcat ttgatacagc tgctgctcaa ggggcagtga ttaaagtaat tggtgtcggt
    64501 ggaggtggtg gcaatgccat caaccgtatg gtcgacgaag gtgttacagg cgtagaattt
    64561 atcgcagcaa acacagatgt acaagcattg agtagtacaa aagctgagac tgttattcag
    64621 ttgggaccta aattgactcg tggtttgggt gcaggaggtc aacctgaggt tggtcgtaaa
    64681 gccgctgaag aaagcgaaga aacactgacg gaagctatta gtggtgccga tatggtcttc
    64741 atcactgctg gtatgggagg aggctctgga actggagctg ctcctgttat tgctcgtatc
    64801 gccaaagatt taggtgcgct tacagttggt gttgtaacac gtccctttgg ttttgaagga
    64861 agtaagcgtg gacaatttgc tgtagaagga atcaatcaac ttcgtgagca tgtagacact
    64921 ctattgatta tctcaaacaa caatttgctt gaaattgttg ataagaaaac accgcttttg
    64981 gaggctctta gcgaagcgga taacgttctt cgtcaaggtg ttcaagggat taccgatttg
    65041 attaccaatc caggattgat taaccttgac tttgccgatg tgaaaacggt aatggcaaac
    65101 aaagggaatg ctcttatggg tattggtatc ggtagtggag aagaacgtgt ggtagaagcg
    65161 gcacgtaagg caatctattc accacttctt gaaacaacta ttgacggtgc tgaggatgtt
    65221 atcgtcaacg ttactggtgg tcttgactta accttgattg aggcagaaga ggcttcacaa
    65281 attgtgaacc aggcagcagg tcaaggagtg aacatctggc tcggtacttc aattgatgaa
    65341 agtatgcgtg atgaaattcg tgtaacagtt gttgcaacgg gtgttcgtca agaccgcgta
    65401 gaaaaggttg tggctccaca agctagatct gctactaact accgtgagac agtgaaacca
    65461 gctcattcac atggctttga tcgtcatttt gatatggcag aaacagttga attgccaaaa
    65521 caaaatccac gtcgtttgga accaactcag gcatctgctt ttggtgattg ggatcttcgc
    65581 cgtgaatcga ttgttcgtac aacagattca gtcgtttctc cagtcgagcg ctttgaagcc
    65641 ccaatttcac aagatgaaga tgaattggat acacctccat ttttcaaaaa tcgttaagta
    65701 aatgaatgta aaagaaaata cagaacttgt ttttcgagaa gttgcagagg ctagtctgag
    65761 tgctcatcga gagagtggtt cggtctctgt cattgcagtt accaagtatg tagatgtacc
    65821 gacagcggaa gccttgcttc cgctaggtgt tcatcatatc ggtgaaaatc gtgtagataa
    65881 gtttctggaa aaatatgaag ctttaaaaga tcgagatgtg acttggcatt tgattggtac
    65941 cttgcaaaga cgtaaggtga aagatgtcat tcaatacgtt gattatttcc atgcattgga
    66001 ctcagtaaag ctagcagggg aaattcaaaa aagaagtgac cgagtcatca agtgtttcct
    66061 tcaagtaaat atttctaaag aagaaagcaa acacggtttt tcgagagagg aactgctgga
    66121 aatcttgcca gagttagcca gactagataa gattgaatat gttggtttaa tgacgatggc
    66181 accttttgag gctagcagtg agcagttgaa agagattttc aaggcgaccc aagatttaca
    66241 aagagaaatt caagagaaac aaattccaaa tatgcctatg accgagttaa gtatgggaat
    66301 gagtcgtgat tataaagaag cgattcaatt cggttccact tttgttcgta taggtacatc
    66361 attttttaag taggagagaa ccatgtcttt aaaagataga ttcgatagat ttatagatta
    66421 ttttacggag gatgaggatt caagtctccc ttatgaaaaa agagatgagc ctgtgtttac
    66481 tccagtaaat tcttcacagg aaccggctct cccaatgaat caaccttcac agtcggctgg
    66541 tacaaaagag aacaatatca ccagacttca tgcaagacaa caggaattgg caaatcagag
    66601 tcagcgtgca acggataagg tcattataga tgttcgttat cctagaaaat atgaggatgc
    66661 aacagaaatt gttgatttat tggcaggaaa cgaaagtatc ttgattgatt ttcagtatat
    66721 gacagaggtg caggctcgtc gttgtttgga ctatttggat ggagcttgtc atgttttagc
    66781 tggaaatttg aaaaaggtag cttctaccat gtatttgttg acaccagtga atgttattgt
    66841 aaatgttgaa gatatccgtt taccagatga agatcaacag ggtgagttcg gttttgatat
    66901 gaagcgaaat agagtacgat aatgattttt ttaattcgta tgatttataa tgcagtggat
    66961 atttactccc tgattttggt agccttcgct gtcatgtctt ggtttccagg tgcctacgaa
    67021 tccagtttag gtcgttggat tgtagcgttg gtgaaaccag tgcttgctcc cttgcaacgc
    67081 ctgcctttac agatagcggg tcttgattta tctgtttggg tcgcgattgt tttggttcga
    67141 tttttaggag aaaacctagt gcgttttctg gcgatgatag gatgaataaa gggatttatc
    67201 agcatttctc catagaagat cgtccatttc ttgacaaggg aatggaatgg ataaagaagg
    67261 tagaagatag ctatgctcct tttttaactc cttttatcaa tcctcatcag gagaagctat
    67321 taaagatttt ggccaaaacc tatggtcttg cttgtagcag tagtggggaa ttcgtctcga
    67381 gtgagtatgt tcgagtttta ttatacccag attatttcca accagagttt tcagattttg
    67441 aaatatctct ccaggaaatt gtgtattcca ataaatttga acatttaacg catgctaaga
    67501 ttttagggac agtcatcaat caattaggga ttgaacggaa actttttgga gatatcctag
    67561 tagatgaaga acgggcgcag attatgatta atcagcagtt tcttcttctc tttcaagatg
    67621 gactaaagaa aattggtcgt atacctgttt cgctggagga acgtcctttc accgagaaaa
    67681 tagataagct agaacagtat cgagaactgg atttatctgt gtctagtttt cgattagatg
    67741 ttctttgttc aagttggaga cttgattagt gtgagaaaat ttggtcgctt gagattactt
    67801 caagataagg gacaaacgaa aaaagagaag aaaaaaataa ccgtccagtt attattaagt
    67861 aagtgaggaa tagaatgcca attacatcat tagaaataaa ggacaagact tttggaactc
    67921 gattcagagg ttttgatcca gaagaagtcg atgaattttt agatattgtg gttcgtgatt
    67981 acgaagatct tgtgcgtgcg aatcatgata aaaatttgcg tattaagagt ttagaagagc
    68041 gtttgtctta ctttgatgaa ataaaagatt cattgagcca gtctgtattg attgctcagg
    68101 atacagctga gagagtgaaa caggcggcgc atgaacgttc aaacaatatc attcatcaag
    68161 cagagcaaga tgcgcaacgc ttgttggaag aagctaaata taaggcaaac gagattcttc
    68221 gtcaagcaac tgataatgct aagaaagtcg ctgttgaaac agaagaattg aagaacaaga
    68281 gccgtgtctt ccaccaacgt ctcaaatcta caattgagag tcagttggct attgttgaat
    68341 cttcagattg ggaagatatt ctccgtccaa cagctactta tcttcaaacc agtgatgaag
    68401 cctttaaaga agtggttagc gaagtacttg gagaaccgat tccagctcca attgaagaag
    68461 aaccaattga tatgacacgt cagttctctc aagcagaaat ggaagaatta caagctcgta
    68521 ttgaggtagc cgataaagaa ttgtctgaat ttgaagctca gattaaacag gaagtggaaa
    68581 ctccaactcc tgtagtgagt cctcaagttg aagaagagcc tctgctcatc cagttggccc
    68641 aatgtatgaa gaaccagaag tagctccagt gcatccgaca ggtccaacac cagctacaga
    68701 aactgttgat tcagcaccgg gatttgaagc accgcaagaa tctgttacaa ttttataaga
    68761 aatattctga gaacaatatc ttatccttat attcccagcg agcaggagat ggtgtgagtc
    68821 ctgcattccc tatcgataag attatcctct caaactatca agtctgaatc tagtaagatt
    68881 tgacgttccc cacgttacgg gataagaggg agaaagacta aaactttttc cgaataaagg
    68941 tggtaccacg attttcgtcc tttttggaag tcgtggtttt taatttgtta ttatttataa
    69001 aggagatacc atgaaactca aagacaccct taatcttggg aaaactgaat tcccaatgcg
    69061 tgcaggcctt cctaccaaag agccagtttg gcaaaaggaa tgggaagatg caaaacttta
    69121 tcaacgtcgt caagaattga accaaggaaa acctcatttc accttgcatg atggccctcc
    69181 atacgctaac ggaaatatcc acgttggaca tgctatgaac aagatttcaa aagatatcat
    69241 tgttcgttct aagtctatgt caggatttta cgcaccattt attcctggtt gggatactca
    69301 tggtctgcca atcgagcaag tcttgtcaaa acaaggtgtc aaacgtaaag aaatggactt
    69361 ggttgagtac ttgaaacttt gccgtgagta cgctctttct caagtagata aacaacgtga
    69421 agattttaaa cgtttgggtg tttctggtga ctgggaaaat ccatatgtga ccttgactcc
    69481 tgactatgaa gcagctcaaa ttcgtgtatt tggtgagatg gctaataagg gttatatcta
    69541 ccgtggtgct aagccagttt actggtcatg gtcatctgag tcagcccttg ctgaagcaga
    69601 gattgaatac catgacttgg tttcaacttc cctttactat gccaacaagg taaaagatgg
    69661 caaaggagtt ctagatacag atacttatat cgttgtctgg acaacgactc catttactat
    69721 cacagcctct cgtggtttga cggttggtgc agatattgat tacgttttgg ttcaacctgc
    69781 tggtgaagct cgtaagtttg tcgttgctgc tgaattattg actagcttgt ctgagaaatt
    69841 tggctgggct gatgttcaag ttttggaaac ttaccgtggc caagaactca accacatcgt
    69901 aacagaacac ccatgggata cagctgtaga agagttggta attcttggtg accacgttac
    69961 gactgactct ggtacaggta ttgtccatac agcccctggt tttggtgagg acgattacaa
    70021 tgttggtatt gctaataatc ttgaagtcgc agtgactgtt gatgaacgtg gtatcatgat
    70081 gaagaatgct ggtcctgaat ttgaaggtca attctatgaa aaggtagttc caactgttat
    70141 tgaaaaactt ggtaacctcc ttcttgccca agaagaaatc tctcactcat atccatttga
    70201 ctggcgtact aagaaaccaa tcatctggcg tgcagttcca caatggtttg cctcagtttc
    70261 taaattccgt caagaaatct tggacgaaat tgaaaaagtg aaattccact cagaatgggg
    70321 taaagtccgt ctttacaata tgatccgtga ccgtggtgac tgggttatct ctcgtcaacg
    70381 tgcttggggt gttccacttc ctatcttcta cgctgaagat ggtacagcta tcatggtagc
    70441 tgaaactatt gaacacgtag ctcaactttt tgaagaacat ggttcaagca tttggtggga
    70501 acgtgatgcc aaagacctct tgccagaagg atttactcat ccaggttcac caaacggcga
    70561 gttcaaaaaa gaaactgata tcatggacgt ttggtttgac tcaggttcat catggaatgg
    70621 agtggtggta aaccgtcctg aattgactta cccagccgac ctttacctag aaggttctga
    70681 ccaataccgt ggttggttta actcatcact tatcacatct gttgccaacc atggcgtagc
    70741 accttacaaa caaatcttgt cacaaggttt tgcccttgat ggtaaaggtg agaagatgtc
    70801 taaatctctt ggaaatacca ttgctccaag cgatgttgaa aaacaattcg gtgctgaaat
    70861 cttgcgtctc tgggtaacaa gtgttgactc aagcaatgac gtgcgtatct ctatggatat
    70921 cttgagccaa gtttctgaaa cttaccgtaa gattcgtaac actcttcgtt tcttgattgc
    70981 caatacatct gactttaacc cagctcaaga tacagtcgct tacgatgagc ttcgttcagt
    71041 tgataagtac atgacgattc gctttaacca gcttgtcaag accattcgtg atgcttacgc
    71101 aaactttgaa ttcttgacaa tctacaaggc cttggtgaac tttatcaacg ttgacttgtc
    71161 agccttctac cttgattttg ccaaagatgt tgtttacatt gaaggtgcca aatcactgga
    71221 acgccgtcaa atgcagactg tcttctatga cattcttgtc aaaatcacca aactcttgac
    71281 tccaatcctt cctcacactg cggaagaaat ctggtcatat cttgagtttg aagccgaaga
    71341 cttcgtccaa ttgtcagaat taccagaagt tcaaactttt gctaaccaag aagaaatctt
    71401 ggatacatgg gcagccttca tggacttccg tggacaagca caaaaagcct tggaagaagc
    71461 tcgtaatgca aaagttatcg gtaaatcact tgaagcacac ttgacagttt atccaaatga
    71521 agttgtgaaa actctactcg aagcagtaaa cagcaatgta gctcaacttt tgatcgtgtc
    71581 tgagttgacc atcgcagaag aaccagctcc ggaagctgcc cttagcttcg aagatgtagt
    71641 cttcacagtt gaacgcgctg caggtgaagt atgtgaccgt tgccgtcgta ttgacccaac
    71701 aacagcagaa cgtagctacc acgcagtcat ctgtgaccac tgtgcaagta tcgtagaaga
    71761 aaactttgca gatgcagtcg cagaaggatt tgaagagaaa taagattgaa aagtctaggt
    71821 aaaactcaat ttgagaagaa aagacaacta attttatagt ctattaaacg cattgtatca
    71881 cgtttttgaa cacctgatat gatgcgtttt ttatttattt taaaaatttg cgaggtatga
    71941 ctttttatac tcaacaagaa tcaaagagaa acttagcaag ctaacagtag taatataaaa
    72001 taggaatttg atattaggga taagattggt aaatagtgta atatttttac aacaataaat
    72061 ttatatagtt atttctggtt tctgaaaagt attatattat attttatttc atattataca
    72121 aatttttatt ttataatatc agaaaatact ttttttaaaa agcaaatatg atacaatttt
    72181 atttgaaaaa aaataaaaaa ggagatttta ttatgaaatc aagtggtatc gtcggtcttg
    72241 tgggaggaat tttacttctt atttgtcctt ttgtcttgtt gggaacagcg gtaaatacag
    72301 ctgctacaac tcttaatgga ggagctactg caggggcttt ttcaggtgtt gccttactct
    72361 tgaatgcctt gaagattgca aatcttgttc ttggtatcat tgctattgtt tactataaag
    72421 gagataagca tgtaggtgca gctccgtctg tactaatgat tgtttctggt gaagttagtc
    72481 tcattccatt cttaggatgg gttgggggga ttcttgctat tatcggagga tctctattcc
    72541 ttgcaacatt gaagaaattc aaatcagaag aataaaaggt attttagcat gaaaagaaca
    72601 aaaaagttta tcggtatagg agtagctcta ttatctcttt ctcttctagt tgcatgtgga
    72661 acataaagtt caaagaatac ttcaacaagt aatgatgaga agacagtagc aacatccaat
    72721 agttcaaaag aaacaatcac tttcgataca ccggttgtaa cagacgatgc gattgaatca
    72781 atacgcactt atgcagatta tatagatctt tataaaaata tttttgatga ttattttact
    72841 aaagctgagg aaggtttcaa aggcacaact atggaaaata atgactcgtt tactaaacta
    72901 aaagagtcaa ctcaaaaatt attcgatgcg cagaaaaaaa ggttaaataa tgaagataga
    72961 atagaaacaa ccaaaaacaa tgtgattgcc aaacattgtc aaacagtcct ttcctttttg
    73021 gttttgacta gcttttttgt gaaaaattgt gtaaaataga atagataaac gaggggaaac
    73081 ctcggaaaat ttaaaggaga atccatctaa tggtaaaatt ggtttttgct cgccacggtg
    73141 agtctgaatg gaacaaagct aaccttttca ctggttgggc tgatgttgat ttgtctgaaa
    73201 aaggtacaca acaagcgatt gacgctggta aattgatcaa agaagctggt atcgaatttg
    73261 accaagctta cacttcagta ttgaaacgtg ctatcaaaac aactaacttg gctcttgaag
    73321 cttctgacca attgtgggtt ccagttgaaa aatcatggcg cttgaacgaa cgtcactacg
    73381 gtggtttgac tggtaaaaac aaagctgaag ctgctgaaca atttggtgat gagcaagttc
    73441 acatctggcg tcgttcatac gatgtattgc ctccaaatat ggaccgtgat gatgagcact
    73501 cagctcacac agaccgtcgt tatgcttcac ttgacgactc agtcattcca gatgctgaaa
    73561 acttgaaagt gactttggaa cgtgctcttc cattctggga agataaaatc gctccagctc
    73621 ttaaagatgg taaaaacgta ttcgtaggag ctcacggtaa ctcaatccgt gcccttgtaa
    73681 aacacatcaa aggtttgtca gatgatgaga tcatggacgt ggaaatccct aacttcccac
    73741 cattggtatt cgaattcgac gaaaaattga acgttgtttc tgaatactac cttggaaaat
    73801 aaaaaattgt aagcctagga ttgatttcta ggctttttat gttagtatgg aagtatgata
    73861 aggaataaaa aacaagatta tgtactggcc tacaagcaac cagcttcaac cacttacatg
    73921 ggttgggaag aagaagcttt accgataggc aatggttctt taggagcaaa agtatttggc
    73981 cttatagggg ctgaacggat tcaatttaat gaaaaaagtc tctggtctgg aggtccactt
    74041 cctgatagtt cagattatca gggtggaaat cttcaggatc agtatgtttt tttagctgag
    74101 attcggcagg ctttggagaa gagagattac aatctggcta aggaactggc tgagcagcac
    74161 ctaattgggc caaaaacgag tcaatatggg acctatctgt cttttgggga tattcacatt
    74221 gagttcagcc agcaaggtac gactttgtct caggtgacgg actatcagag acagctgaat
    74281 attagtaagg cacttgcgac gacttcttat gtctataagg gaacgcgatt tgaacgtaaa
    74341 gcttttgcga gttttccaga tgatctcttg attcaatgtt ttactaagga agggttggaa
    74401 actctagatt ttactataga actatccttg acctgtgatt tggcttctga tggaaagtat
    74461 gagcaggaaa aatctgatta caaggagtgt aagttggata ttactgattc tcatatcttg
    74521 atgaagggaa gagttaagga taatgatctg cggtttgcta gttatctagc ttgggaaacg
    74581 gatggagata ttagagtttg gtcagatagg gttcagatat caggagccag ttatgccaat
    74641 ctcttcttgg ccgctaagac ggattttgcc caaaatcctg ctagcaatta tcgcaagaaa
    74701 ctagatttag agcaacaggt gatagacttg gtggacacag ctaaagaaaa gggctatacc
    74761 caattgaaat caaggcatat cgaggactac caagccttat tccagcgtgt tcaattggat
    74821 ttggaagctg atgttgacgc atccactaca gatgatttgt taaaaaatta taagccacaa
    74881 gaagggcagg ctttggagga gctgttcttc cagtatggac ggtatttatt gattagttcg
    74941 tccagagact gcccagatgc tctaccagct aacctacagg gagtctggaa tgcggtcgac
    75001 aatcctcctt ggaattcgga ctatcactta aatgtcaatc tgcagatgaa ttattggcca
    75061 gcctatgtta ccaatctcct agagacggtc tttccagtca tcaactatgt agatgatttg
    75121 cgtgtctatg gtcgtctagc ggctgtaaag tatgcaggaa tcgtctctca aaaaggtgag
    75181 gaaaatggtt ggttggttca tactcaagcg actccctttg gttggacggc acctggttgg
    75241 gattactatt ggggttggtc accagctgcc aatgcgtgga tgatgcaaac cgtttatgaa
    75301 gcctattcat tttataggga ccaagactat ctcagggaga aaatttatcc catgttgagg
    75361 gaaacggttc gtttttggaa tgccttttta cataaggatc agcaggcgca gcgttgggtc
    75421 tcttctccgt cttattcccc agaacatggg ccgatttcga ttggcaatac ctatgaccaa
    75481 tctctgattt ggcagttatt tcatgatttt attcaggctg ctcaggaatt gggactggat
    75541 gaggacttgt tgactgaggt taaggagaag tctgatttac taaatccttt gcaaatcact
    75601 caatctggtc gaatcaggga gtggtatgag gaggaagagc agtattttca aaatgagaaa
    75661 gtggaggccc agcatcggca cgcttcccat ctagtgggac tctattctgg caatctcttt
    75721 agctacaagg gacaagagta tattgaagcg gcgcgtgcta tcctcaatga tcgtggagat
    75781 ggcggcacag gctggtccaa ggctaataag atcaatctct gggcgcgttt gggagatggc
    75841 aatcgagccc ataaattatt ggcagagcag ttaaagacat ccaccttgcc aaatctttgg
    75901 tgtagccatc ctccttttca gatagatggt aattttggtg ctactagtgg catggcagaa
    75961 atgttactcc agtctcatgc agcttatctg gtacctctag ctgccctacc tgatgcttgg
    76021 tcaacaggtt ctgtttcagg cttaatggca cgtggacatt ttgaagtgag catgagctgg
    76081 gaagataaaa aactcttaca gttgaccatt ttatcaagga gtggaggaga tttgcgagtt
    76141 tcttatccag atattgagaa gagtgtgatt aaaatgaatc aagaaaaaat aaaagcgaaa
    76201 tgcatgggga aagattgtat ttcggtggca acagcagaag gtgatcttgt tcaattttat
    76261 ttttaagaag atgttataag gcagtaattt gaaacttcct tttaataagg atttgagaat
    76321 ataggcagtt tccaactagt tgaaaaagcg ttataatgat aataggaagt aatactcaat
    76381 gaaaatcaaa gagcacaaac taggaagcta gccgcaggtt gctcaaaaca gtgttttgag
    76441 gttgtagatg gaagctgacg tggtttgaag agagattttc gaggagtata atttgtttga
    76501 tagagggtgg gtctgatggc ttatattgag atgaaacact gttacaagcg ttatcaggtt
    76561 ggggacacgg agattgtggc caatcgtgat gtgaattttg agattgaaaa gggggagctg
    76621 gttattatcc ttggtgcttc aggtgcaggc aagtcaacag ttcttaacct tcttggggga
    76681 atggatacca atgatgaagg ggaaatctgg attgatggtg ttaatattgc ggattatagt
    76741 tcccaccagc gtaccaatta ccgtagaaat gatgtggggt ttgtttttca gttttataat
    76801 ctagtttcta atctgacagc taaggaaaat gtggaactgg cttctgaaat tgtgacagat
    76861 gccttgaatc ctgatcaggt cttgacagat gtaggtctgg ctcatcgtct caataacttt
    76921 ccagcccagc tttctggagg ggagcaacag cgagtctcca ttgcacgcgc ggtagccaaa
    76981 aatcctaaaa ttctcctttg tgatgaaccg actggagcct tggattatca gacgggcaag
    77041 caggttttga aaattctcca agacatgtct cgtcaaaagg gagcgacggt gatcatcgtg
    77101 actcataatg gagctttggc gcccattgct gatcgcgtga ttcaaatgca cgatgccagt
    77161 gtcaaggatg tggtgctcaa ccagcatcct caggatattg acagtttgga gtactagcat
    77221 gatcaagcga aaaacttatt ggaaggactt aattcagtcc ttcacaggct ccaaggggcg
    77281 ttttttatcc atcttgatcc tgatgatgtt gggatctcta gccttagtag gcctcaaagt
    77341 aaccagtccc aacatggagg cgacagctaa tgcttattta acaactgctc aaaccttgga
    77401 tttggcagtc atgtctaact atggcttgga tcaagcagac caagaagaac taaaacagac
    77461 ggagggcgca gaggtcgagt ttggctattt gacagatgtg actatggata atgggcagga
    77521 tgccattcgg ctgtactcca aaccagagcg aatttcaacc tttcagctaa gaaagggacg
    77581 acttcctcag tcagacaagg aaatcgcttt ggccactcat ttgcaaggcc aatacagcgt
    77641 gggacaggag attagtttta aagaaaaaga agagggtcat tcctctttaa aagaccatac
    77701 ttataccatt actggttttg tggattcggc tgaaatcctc tcccagcgag atatgggcta
    77761 cgcaggaagt ggaagtggga ctctgacagc ctatggggtg attttaccta gtcagtttga
    77821 tcagaaagtc tacaatatag ctcgtttgaa atatcaagat ttagcaggtt taaatgcctt
    77881 ttcatcagct tatgaagaaa aatccaagca acatcaggaa gaccttgaac aaactttatc
    77941 agataatggc aaggtacgtc tgcaactttt gaaaaaagaa ggacaagagt ctctagacaa
    78001 ggggcaagag acccttgaca aggctcagac taatttgcag gaaggcaagc gtcgtttagc
    78061 agctgctcaa gctcgtatac aggctcaaga aagtcaacta gccttgtttc ctcaagttca
    78121 gagagagcag gctagtgctc aacttaccca agccaagcag gaattgggca aggaagagga
    78181 taaactaaag caagctgaac aaaatctagc ccaagaaaag gaaaaattag aaaaacatca
    78241 gcaagtcttg gacgatttgg cggagccaag gtatcaggtc tataatcgtc agaccatgcc
    78301 aggtggtcag ggctatctta tgtatagcaa tgcttcatcc agtattcgag cagtgggcaa
    78361 tatctttcct gtggtacttt atgccgtagc atccatggtg acctttacga ccatgactcg
    78421 ctttgtagac gaagagcgaa ctcatgcagg gatttttaag gccttgggtt accgtagtaa
    78481 ggatattatc accaagtttc tcctttatgg actagtagct gggactgtcg gaacggctct
    78541 aggtagtata cttggtcatt atttgctagc cagtgtaatt tcaagtgtca ttacaaaagg
    78601 catggtggtg ggagaaaccc agattcagtt ctattggacc tatagcttac tagctcttgt
    78661 cttgagctgg ttggcgagtg tgttaccagc ctatctggtg gcttggaggg aacttcatgc
    78721 cgaagcagcc cagcttctac ttcctaaacc tcctgtcaaa ggagctaaaa tcttattgga
    78781 gcgtatcggt tttatctggc gtcgtctcag ttttactcat aaggtaacag cccgcaacat
    78841 ctttcgttat aagcagagaa tgttgatgac aatctttggt gtggcaggtt ctgtagctct
    78901 gctctttgca ggtttgggaa tccaatcttc tgtagcagga gttccgtcta aacagtttca
    78961 acaaatccaa cagtatcaga tgcttgtctc tgaaaatcct agtgcgacca atcaggacaa
    79021 ggtagagcta gcagaagtgt tgaaagggca ggagatacta gcctaccaga aaatctattc
    79081 taaaacgcta gacaaggatt tcaaaggcaa ggctggtctt caaaacatta ctcttatgat
    79141 gatagagaag gaagatttga ctccctttat ccatcttcaa catcatcagc aggagctgac
    79201 attaaaagat ggcatcgtta ttacagctaa actcgcccag ctggcaggtg tcaaggttgg
    79261 gcagacttta gaaattgaag gtaaggaact aaaggtcgct gctattactg agaactacgt
    79321 tggtcacttt atttatatga gtcaggctag ctatgagcaa ctttacggac agctacccca
    79381 agccaacact tatctggtct cattaaggga taccagtgca actagtatcg aaagtcaggc
    79441 gggcttgctt atgaatcaat ctgcggtgtc cagcgttgtc caaaatgctt cagccattcg
    79501 actctatcgc tagctcactc aatcagacca tgaccatctt ggtcatcgta tcagttctat
    79561 tagctattgt catcctttac aatctgacca atatcaacgt agctgagaga atccgtgaac
    79621 tctccactat caaggttctc ggttttcata ataatgaagt caccctctac atttaccgtg
    79681 agacgattgt gctgtccctt gtgggaatcg tacttggtct ggtagctggt ttctatttac
    79741 accaattttt gattcaaatg atttcgcctg cgactattct cttttatccg caggtaggct
    79801 gggaagtcta tgtaatccca gtggcaacag taagcatcat tttgaccttg cttggtttct
    79861 tcgtcaatta ttatctgaga aaggttgata tgttagaagc cctgaaatct gtagagtaag
    79921 gtagttattt ttagctgatt gaacttctat ttactaatat tcaaaaatcc tctgtcacaa
    79981 agagcaggga gctctttgtg acagaggatt ttttctatag ggctttagca gctgcaattg
    80041 cggcttcgaa gtttggctca gaattgatat tatccacgta ttcaacgtag cgaatcgtat
    80101 tgtcagtatc gaggacaaag actgcgcgtg ctaataggtg ccattcgttg atcaagaggg
    80161 cataatcgcg cccgaaagaa tggtcaaagt agtctgaaag cataatggca ttgtcaaggc
    80221 cttcagcacc gcaccaacgt ttttgagcaa aaggtaggtc cattgaaaca gtcaatacga
    80281 ccgtgttgtc cagtccagcc aattcttcat taaaacgacg tgtttgagtt gagcagatgc
    80341 ctgtatcgat agaaggaacg acactcaaga cttttttctt gccatcaaaa tcagccagag
    80401 attttttaga aagatctgtt gtagtaagag aaaaatcaag cgccttgtcg ccgacttgta
    80461 gttgtttacc tgtaaagctc acaggatttc cgagaaaagt taccatagga tactccaatc
    80521 ttttttcttc cattgtatct gaaacagtca gaattttcca atgatttgac cggaaatgtg
    80581 ggcatagaaa aaacgccagc tcacatgaga atgacgtttt tcagaggctt attttgccaa
    80641 tccttcagca atcttgtcaa ggttgtattt catcatgctg tagtagctgt cgccttcttt
    80701 accttgttct gcgatagagt cagtaaagat ttgtgcgtag attgggatgt ttgtgtcttg
    80761 agaaacagtt ttcattggac ggtcatccac acttgattct acaaagagtg atggaacttt
    80821 tgtttggcga agtttttcaa ccaaggtctt gatttgttca ggagttcctt cttcttcagt
    80881 attgatttcc cagatgtaag cacttgggac accataggct ttagagaagt atttgaatgc
    80941 tccttcgctg gttacaatga gtttcttttc agcagggatc ttattaaatt tatccttact
    81001 ttctttatca agtttgtcta acttatcagt atattctttg agattttttt catagaattc
    81061 tttattgtta gggtctttgg cgctcaattg tttggcgata tttttagcaa aaataatacc
    81121 gttttcaagg ttaagccaag cgtgtgggtc ttcttttcct ttttcatttt gaccttcaag
    81181 gtagataaca tcaacgccgt cgctgactgc gaagtagtct ttgttttcag ttttcttggc
    81241 attttctacc aattttgtaa accaagcatt gccacctgtt tcaaggttga taccgttata
    81301 gaaaatcaaa tcagcctcag aagttttctt aacgtcttca ggaagtggtt cgtattcgtg
    81361 tgggtcttgc ccaatcggaa cgatactatg aaggtcaatt ttgtcaccag caatattttt
    81421 agtaatatca gcgatgattg agtttgtagc aacaactttt agtttttgac cagaagttgt
    81481 atcttttttt ccgctagcac atgctacaag aatgattgca gaaagaaaga gaacgagtaa
    81541 tgtacctaat tttttcatta gatcctccaa attattaggg ctttgcccct tattttaaca
    81601 aatgtttatt tttcagtttc aaatatcgtt gtttgggagc gataaagaag ctaatgagaa
    81661 agaaactagc agctgtaagc acgatactag aacctgccgc aacattaaaa ctatagccaa
    81721 taaagagtcc caaaactgaa gcagtagctc cgaaggttga ggaaaggaaa atcatgcttt
    81781 tcagactatt agcatacaga taagcagttg cagctggggt aatcagcatg gctacaatca
    81841 ggatagttcc gacactttgc atggctgtca cagacacgag agtcaggagt accatgagaa
    81901 ggtagtgata gaaattgaca ggcattccca tggctttagc caagagttca tcaaaggaag
    81961 ttatcaagag ttgcttgaag aaaatccaga ttaacaagag gatggctgcc cccacaccca
    82021 tagtaataaa catatccgta tcttggacgg ccaggatatt accaaaaagg atatggaaaa
    82081 ggtcagttga acttttagcg acaccaatca agatgatacc gagggctaag aaagaagaaa
    82141 aggtaatgcc gatggcggta tcgcttttga taatcgagtt tcctttgatg taggtaatga
    82201 tgatggcagc tagcaatcca aagacaatgg ctccgataaa gaagtcaagg cccaagatga
    82261 aggagagggc tacacctggt aagacagcat gtgaaatggc atctcccatg agtgacatcc
    82321 cgcgtagaat aatgaaacat cccacagctc cagctacaat cccgacgaca atagctgtta
    82381 tcaaggcatt ttgtaggaaa tggaattttt gcaatccatc gataaattct gcaatcatag
    82441 gtcacctcca ttgaaaaaga gttgattacc gtaagcttct tttagattgg tttcggtaaa
    82501 agtttctttt gttggaccaa aggcaatcac ttctcgattg acaagtaaga cttgatcgaa
    82561 gtagtgggga atcttgctga ggtcgtggtg aacgatgaga accgtcttcc cagctttttt
    82621 caaatctctc agcgtattca tgatgatttc ctcactgaca gagtcaatcc cagcaaaggg
    82681 ttcatccaag aggatatagt cggcttcctg caccaaacat ctggcaatca aaacccgctg
    82741 gaattgacct ccagacagtt gactaatttg acgttcagcg tagtcagcta ggccgacgat
    82801 ttcaagggcc tcttgcactt tcttccaatg tttagccttt aaacttcgaa agagaggaat
    82861 agagggaaat agtcctaacg agacgcattc cttgaccttg atgggaaagt tgtagtcgat
    82921 attgattttt tgttcgacat aggcaattcg gtgtaaggat tttttaactt ccttgtcatc
    82981 gagaaatgcc tgaccttgat gtgggataat tcccagcata ccttttaata gtgttgattt
    83041 cccagcgccg tttggaccaa tgatgccggt aattgttggt ccatggagca ctagtgaaat
    83101 atccttaagt gccaacgttt ctttgtagga gacactgagg ttttcgatac gtatcataaa
    83161 cttgtattcc tcctgtctct taatatacat taaaaaaaaa attaagtcaa gttaattttt
    83221 gaaaaaatta aaataataac tgaaaaatag a
//
LOCUS       DAVBCV010000002        80116 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBCV010000002 DAVBCV010000000
VERSION     DAVBCV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 80116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.002"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(134..1882)
                     /locus_tag="VQ516_000076"
     CDS             complement(134..1882)
                     /locus_tag="VQ516_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510599.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTISRVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1884..3608)
                     /locus_tag="VQ516_000077"
     CDS             complement(1884..3608)
                     /locus_tag="VQ516_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510600.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(3666..4604)
                     /gene="msrB"
                     /locus_tag="VQ516_000078"
     CDS             complement(3666..4604)
                     /gene="msrB"
                     /locus_tag="VQ516_000078"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2510601.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(4746..4823)
                     /locus_tag="VQ516_000079"
                     /pseudo
     CDS             complement(4746..4823)
                     /locus_tag="VQ516_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4899..5768)
                     /locus_tag="VQ516_000080"
     CDS             complement(4899..5768)
                     /locus_tag="VQ516_000080"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2510602.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTKGVRVEAK"
     gene            complement(5770..7056)
                     /locus_tag="VQ516_000081"
     CDS             complement(5770..7056)
                     /locus_tag="VQ516_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2510603.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(7207..7944)
                     /gene="mecA"
                     /locus_tag="VQ516_000082"
     CDS             complement(7207..7944)
                     /gene="mecA"
                     /locus_tag="VQ516_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2510604.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(8144..9178)
                     /locus_tag="VQ516_000083"
     CDS             complement(8144..9178)
                     /locus_tag="VQ516_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2510605.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(9207..10640)
                     /locus_tag="VQ516_000084"
     CDS             complement(9207..10640)
                     /locus_tag="VQ516_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2510606.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            complement(10656..11642)
                     /locus_tag="VQ516_000085"
     CDS             complement(10656..11642)
                     /locus_tag="VQ516_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2510607.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(11644..12747)
                     /locus_tag="VQ516_000086"
     CDS             complement(11644..12747)
                     /locus_tag="VQ516_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2510608.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQIIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(12737..13582)
                     /locus_tag="VQ516_000087"
     CDS             complement(12737..13582)
                     /locus_tag="VQ516_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2510609.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLTLDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(13861..15135)
                     /gene="psr"
                     /locus_tag="VQ516_000088"
     CDS             complement(13861..15135)
                     /gene="psr"
                     /locus_tag="VQ516_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2510610.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(15132..15980)
                     /gene="pheA"
                     /locus_tag="VQ516_000089"
     CDS             complement(15132..15980)
                     /gene="pheA"
                     /locus_tag="VQ516_000089"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2510611.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(15977..16453)
                     /locus_tag="VQ516_000090"
     CDS             complement(15977..16453)
                     /locus_tag="VQ516_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2510612.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(16446..17729)
                     /gene="aroA"
                     /locus_tag="VQ516_000091"
     CDS             complement(16446..17729)
                     /gene="aroA"
                     /locus_tag="VQ516_000091"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2510613.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(17821..18159)
                     /locus_tag="VQ516_000092"
     CDS             complement(17821..18159)
                     /locus_tag="VQ516_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2510614.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(18170..19273)
                     /locus_tag="VQ516_000093"
     CDS             complement(18170..19273)
                     /locus_tag="VQ516_000093"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2510615.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(19283..20449)
                     /gene="aroC"
                     /locus_tag="VQ516_000094"
     CDS             complement(19283..20449)
                     /gene="aroC"
                     /locus_tag="VQ516_000094"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2510616.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(20459..21526)
                     /locus_tag="VQ516_000095"
     CDS             complement(20459..21526)
                     /locus_tag="VQ516_000095"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2510617.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(21545..22399)
                     /locus_tag="VQ516_000096"
     CDS             complement(21545..22399)
                     /locus_tag="VQ516_000096"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2510618.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKKMPTEEIW
                     QSLTEKYQ"
     gene            complement(22389..23066)
                     /locus_tag="VQ516_000097"
     CDS             complement(22389..23066)
                     /locus_tag="VQ516_000097"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2510619.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(23063..24226)
                     /locus_tag="VQ516_000098"
     CDS             complement(23063..24226)
                     /locus_tag="VQ516_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2510620.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDERRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(24911..26545)
                     /locus_tag="VQ516_000099"
                     /pseudo
     CDS             complement(24911..26545)
                     /locus_tag="VQ516_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26526..27275)
                     /locus_tag="VQ516_000100"
     CDS             complement(26526..27275)
                     /locus_tag="VQ516_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510621.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            27454..28908
                     /locus_tag="VQ516_000101"
     CDS             27454..28908
                     /locus_tag="VQ516_000101"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2510622.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(28998..31616)
                     /gene="alaS"
                     /locus_tag="VQ516_000102"
     CDS             complement(28998..31616)
                     /gene="alaS"
                     /locus_tag="VQ516_000102"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2510623.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(31638..32123)
                     /locus_tag="VQ516_000103"
     CDS             complement(31638..32123)
                     /locus_tag="VQ516_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2510624.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLLDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(32398..32637)
                     /locus_tag="VQ516_000104"
     CDS             complement(32398..32637)
                     /locus_tag="VQ516_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510625.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILEK"
     gene            complement(32890..33960)
                     /locus_tag="VQ516_000105"
     CDS             complement(32890..33960)
                     /locus_tag="VQ516_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2510626.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNIPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(33957..34730)
                     /locus_tag="VQ516_000106"
     CDS             complement(33957..34730)
                     /locus_tag="VQ516_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2510627.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(34727..35533)
                     /locus_tag="VQ516_000107"
     CDS             complement(34727..35533)
                     /locus_tag="VQ516_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2510628.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(35514..36671)
                     /locus_tag="VQ516_000108"
     CDS             complement(35514..36671)
                     /locus_tag="VQ516_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510629.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(36730..37680)
                     /gene="murB"
                     /locus_tag="VQ516_000109"
     CDS             complement(36730..37680)
                     /gene="murB"
                     /locus_tag="VQ516_000109"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2510630.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(37792..38544)
                     /locus_tag="VQ516_000110"
     CDS             complement(37792..38544)
                     /locus_tag="VQ516_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET2510631.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWFEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(38554..39285)
                     /gene="budA"
                     /locus_tag="VQ516_000111"
     CDS             complement(38554..39285)
                     /gene="budA"
                     /locus_tag="VQ516_000111"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2510632.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            39427..40698
                     /locus_tag="VQ516_000112"
     CDS             39427..40698
                     /locus_tag="VQ516_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2510633.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(40718..41533)
                     /locus_tag="VQ516_000113"
     CDS             complement(40718..41533)
                     /locus_tag="VQ516_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2510634.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(41690..42343)
                     /gene="phoU"
                     /locus_tag="VQ516_000114"
     CDS             complement(41690..42343)
                     /gene="phoU"
                     /locus_tag="VQ516_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2510635.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(42355..43113)
                     /gene="pstB"
                     /locus_tag="VQ516_000115"
     CDS             complement(42355..43113)
                     /gene="pstB"
                     /locus_tag="VQ516_000115"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2510636.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(43126..43929)
                     /gene="pstB"
                     /locus_tag="VQ516_000116"
     CDS             complement(43126..43929)
                     /gene="pstB"
                     /locus_tag="VQ516_000116"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2510637.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(43940..44824)
                     /gene="pstA"
                     /locus_tag="VQ516_000117"
     CDS             complement(43940..44824)
                     /gene="pstA"
                     /locus_tag="VQ516_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2510638.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(44814..45731)
                     /gene="pstC"
                     /locus_tag="VQ516_000118"
     CDS             complement(44814..45731)
                     /gene="pstC"
                     /locus_tag="VQ516_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2510639.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(45731..46609)
                     /gene="pstS"
                     /locus_tag="VQ516_000119"
     CDS             complement(45731..46609)
                     /gene="pstS"
                     /locus_tag="VQ516_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2510640.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(47183..48487)
                     /locus_tag="VQ516_000120"
     CDS             complement(47183..48487)
                     /locus_tag="VQ516_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2510641.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(48488..49261)
                     /locus_tag="VQ516_000121"
     CDS             complement(48488..49261)
                     /locus_tag="VQ516_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2510642.1"
                     /translation="MKSKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(49251..49529)
                     /locus_tag="VQ516_000122"
     CDS             complement(49251..49529)
                     /locus_tag="VQ516_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2510643.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGKGKISLEK"
     gene            complement(49529..49930)
                     /locus_tag="VQ516_000123"
     CDS             complement(49529..49930)
                     /locus_tag="VQ516_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2510644.1"
                     /translation="MITLFLSPSCTSCRKAKTWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(50140..50757)
                     /locus_tag="VQ516_000124"
     CDS             complement(50140..50757)
                     /locus_tag="VQ516_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2510645.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(50747..51520)
                     /locus_tag="VQ516_000125"
     CDS             complement(50747..51520)
                     /locus_tag="VQ516_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2510646.1"
                     /translation="MKYKGYLIDLDGTIYKGKDQIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(51531..52268)
                     /locus_tag="VQ516_000126"
     CDS             complement(51531..52268)
                     /locus_tag="VQ516_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2510647.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEESISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(52273..53403)
                     /locus_tag="VQ516_000127"
     CDS             complement(52273..53403)
                     /locus_tag="VQ516_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2510648.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(53487..53876)
                     /locus_tag="VQ516_000128"
     CDS             complement(53487..53876)
                     /locus_tag="VQ516_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2510649.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(53892..54275)
                     /locus_tag="VQ516_000129"
     CDS             complement(53892..54275)
                     /locus_tag="VQ516_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2510650.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(54276..55064)
                     /locus_tag="VQ516_000130"
     CDS             complement(54276..55064)
                     /locus_tag="VQ516_000130"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2510651.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(55057..55992)
                     /locus_tag="VQ516_000131"
     CDS             complement(55057..55992)
                     /locus_tag="VQ516_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2510652.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLSETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(56122..56298)
                     /locus_tag="VQ516_000132"
     CDS             complement(56122..56298)
                     /locus_tag="VQ516_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2510653.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(56438..57145)
                     /locus_tag="VQ516_000133"
     CDS             complement(56438..57145)
                     /locus_tag="VQ516_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2510654.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            57296..58324
                     /gene="queA"
                     /locus_tag="VQ516_000134"
     CDS             57296..58324
                     /gene="queA"
                     /locus_tag="VQ516_000134"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2510655.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            complement(58995..59384)
                     /locus_tag="VQ516_000135"
     CDS             complement(58995..59384)
                     /locus_tag="VQ516_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2510656.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(59438..60145)
                     /locus_tag="VQ516_000136"
     CDS             complement(59438..60145)
                     /locus_tag="VQ516_000136"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2510657.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            complement(60845..61396)
                     /locus_tag="VQ516_000137"
     CDS             complement(60845..61396)
                     /locus_tag="VQ516_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2510658.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(61794..62618)
                     /gene="nadE"
                     /locus_tag="VQ516_000138"
     CDS             complement(61794..62618)
                     /gene="nadE"
                     /locus_tag="VQ516_000138"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2510659.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(62615..64075)
                     /locus_tag="VQ516_000139"
     CDS             complement(62615..64075)
                     /locus_tag="VQ516_000139"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2510660.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(64191..64679)
                     /locus_tag="VQ516_000140"
     CDS             complement(64191..64679)
                     /locus_tag="VQ516_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510661.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(64669..64878)
                     /locus_tag="VQ516_000141"
     CDS             complement(64669..64878)
                     /locus_tag="VQ516_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2510662.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(65268..65710)
                     /locus_tag="VQ516_000142"
                     /pseudo
     CDS             complement(65268..65710)
                     /locus_tag="VQ516_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66002..66808)
                     /locus_tag="VQ516_000143"
     CDS             complement(66002..66808)
                     /locus_tag="VQ516_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510663.1"
                     /translation="MILKDINFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNI
                     ISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKV
                     KTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILI
                     SSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGD
                     IVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            complement(67214..67942)
                     /locus_tag="VQ516_000144"
                     /pseudo
     CDS             complement(67214..67942)
                     /locus_tag="VQ516_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(68178..68489)
                     /locus_tag="VQ516_000145"
     CDS             complement(68178..68489)
                     /locus_tag="VQ516_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510664.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYVCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(68504..69790)
                     /locus_tag="VQ516_000146"
     CDS             complement(68504..69790)
                     /locus_tag="VQ516_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2510665.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     KGEILEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQNLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQAGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            70414..70596
                     /locus_tag="VQ516_000147"
     CDS             70414..70596
                     /locus_tag="VQ516_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2510666.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            70606..70971
                     /locus_tag="VQ516_000148"
     CDS             70606..70971
                     /locus_tag="VQ516_000148"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2510667.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            70968..72158
                     /locus_tag="VQ516_000149"
     CDS             70968..72158
                     /locus_tag="VQ516_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2510668.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RNGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(72673..73665)
                     /locus_tag="VQ516_000150"
     CDS             complement(72673..73665)
                     /locus_tag="VQ516_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2510669.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            73825..75579
                     /locus_tag="VQ516_000151"
     CDS             73825..75579
                     /locus_tag="VQ516_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510670.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAVLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAEVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAIIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            75563..77284
                     /locus_tag="VQ516_000152"
     CDS             75563..77284
                     /locus_tag="VQ516_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510671.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            77295..77924
                     /locus_tag="VQ516_000153"
     CDS             77295..77924
                     /locus_tag="VQ516_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2510672.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALIALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            77825..78382
                     /locus_tag="VQ516_000154"
     CDS             77825..78382
                     /locus_tag="VQ516_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2510673.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            78370..79734
                     /locus_tag="VQ516_000155"
     CDS             78370..79734
                     /locus_tag="VQ516_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510674.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFIFFDEPSSGIDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT        24632 a        17071 c        14696 g        23717 t
ORIGIN      
        1 acctacggct agcttcctag tttgctcttt gattttcatt gagtattaca gtaaacaatt
       61 tttctagcta ttgtaacaaa aaagtcactc gaaggcaact tttttcgtcc ataaactttc
      121 aaaagagaat ggcttattca gaactaaaag ccgcagcttt ttgcatttgg taatacttac
      181 cctttctatc catgagttct tgatggttac catattcaac aatatcacca tctactaaga
      241 caagaattaa atccgcatcc tgaatggttg acaaacggtg agcaatgatg aagcttgtgc
      301 ggcccttcat gagttttgca aaggcatcct gtaccagcac ttctgtccgt gtatcaatgg
      361 aagaagttgc ctcgtctaag ataagaatct ttggaatagc cagaaagact cgggatatgg
      421 tcaagagctg agcttggccg acagagagag attctccagc attttccaac ttggtatcgt
      481 atccctgtgg caactgttgg atgaaaaagt ctgcattagc tgctttggca gcagcaatta
      541 cttgctctcg actggcttca ggattgccaa aggcaatatt atcatgaatg gtcccttgtg
      601 tgagccaggt ttcttgaagc accataccaa actgctgtct caatgatact cgtgtataat
      661 cataaatgga ttgcccatcc agcaagatat ctcccgagct aatgggataa aaacgcataa
      721 ggagattgat aagagttgat tttccagcac ctgtcggacc aacgatggct accttactac
      781 cagctggaat atcgatagac aactccttaa tcaaaatttt ttcaggatgg tagccaaaag
      841 agacatgttt aaaggaaata gctcccttaa cttggtcact ggtcaagact tccttacctg
      901 tttcagccac ttcagggcta tctaagaccc catagatacg ctctacgcaa gccagagcac
      961 tttgcaactc agctagcact gaagaaatat cgttaaaggg cttggtgtat tgctgaacat
     1021 agttcaaaaa agtcactaaa cgaccgacgg tcaaggttga acccatcatg atacgataag
     1081 ctcctactcc agctaaaagg gcataaatga gtgcatttac aaagcgagtc gaaggattga
     1141 ccgttgaaga ataaaagatg gctgactgag aatagcctga gtagttgtca tgagcctcac
     1201 gcaatctttg gataaattct gtttgagcat tgaaggactg gattatagtc tgctggctaa
     1261 gcgattcttc aatcaactga gtctgaattc ccctcgtctc tgtttgcttc tggaagagat
     1321 gataggatct cttggcaata aagcgtgaaa tcaccatgga cagtggcgtc aacagcaaga
     1381 ctaagagagt catgaggaga tgaatttgga gcatggctag aatactaacc aaaatcatca
     1441 aaacaccaat gaaaaattgg ttaaaaatca tggtcaagcc agctgccaac tgttcgatat
     1501 ccgtggttac acgactgacc atctctccat tgccttgccg atccgcaaaa gcaatcggta
     1561 accgatggag tttatggatg attcgctctc gtaaatctct ggtataagag aagattagac
     1621 gattatagag gagaggattg gcccattgta ccagagtatt tcctattacc accaagagca
     1681 tctggagaaa aatctgccaa aaaactggtg atgaaccagc cactaggact tggtcaatga
     1741 cctgcccaat cagaataggt aggtaaattg ataagccaac ttgggcaata gttcctagaa
     1801 aggctaggaa aaggaggaag ggatggtttg ctaaatctac gactaaacgt ttgagcgtct
     1861 ggtttacagt ttgtcgtttc attctagtcc tcctttccat gttgggatgc attgatttca
     1921 cgatagactt gactggattt catcaagtca tcgtgcttgc caacagctag caactcacct
     1981 ttttccaaga ggagaatctg gtccgccatc tggagtgtcg aagtccgttg agaaatcaag
     2041 attaagctca tatttggcag attttcgcgg atagctttca agagcttgga ctctgtaatg
     2101 gtgtccagtg ctgaggtcgc atcatctagg atgagaaacg gagcctggcg caagactgct
     2161 cgtgcgatag acagcctttg tttttgtcca cctgagaaat ttcgccctcc tgcttcaact
     2221 agagcatcca agagtccttc cttatcactg acaaaatcct tagcttgcgc aatctccaag
     2281 gcttgccaga gttcttgatc agtcacttct tgatttaaac ctaaagtcaa gttggaacga
     2341 atagttccct taaagagttc gaccttttga ggtacatagg caatccaaga ccgccactgc
     2401 tccaaattaa gaggactacg tccattttga taaaggtcaa tgttcccctt gtctactgga
     2461 taaagtccaa gtaagagttg caccaagctt gatttaccag aaccagtccc cccaatgata
     2521 ccaaggattt gtccttgagt catatcaaag gaaatgtctc tcagagaagg ctgggccgca
     2581 tcaggatagg taaaagtcaa ttcttggact tgtaaaaccc gaccactggt aacttgcttt
     2641 tgttctaatt ctgaatggat gttttcggga gattccgcaa agacctcctc gattctcttg
     2701 gctgagatat aggactggtt gagggaattg atcagcatgg ctagcttgac caattccacc
     2761 aaaatctgta agaggtaatt gataagagca atgagagcac cttgactgag tactcctcct
     2821 tgaattgaaa tatagccttg ccagataata acgagaagag ttccattgac aatcagatag
     2881 gtcagaggtg ttaataaact agacaagaaa cctgtctttt cttgtaatct agcataaact
     2941 tggttaaggg tttgaaaaat ctgtaactct cgtttttctt gaccaaaagc acgaataacc
     3001 cgcatccctt gcaattgctg gcgcgtttcc tgaaccagtt ggtccgtttt ctttctgaga
     3061 ctactgtaga gaggattgac caatcgagat aaccctacaa tgacaatggt caaaatggca
     3121 accatgacta agaaccagaa agtcaactca gctgagattc gataagccat aaaaatggca
     3181 ccaaaaacga taatgggcgc tcgtaaaaag agacgcagga attgattgat accagtctga
     3241 atctggtagg tatccgaagt caagcgagta accaaactag aagttgtcag acggtctctg
     3301 ctgtcctttg gcaaggaaag aatatgacga taaagatcgt ttgtcaattc cttagcaaaa
     3361 cctactgctg cctttgctga gtaaaattga gctatcaagg ccactaaaac gccaattact
     3421 gcaaagataa ggagcaggcc aatctgcatc cagagatgac cttgatctcc ctgaggtaaa
     3481 gattggtcaa caatcccagc aatcaccatg ggaaccaaga gctcaaaaac agcttctaac
     3541 agcttgaaca agggggctaa aattgattcc ttgatgtagg gtttgaagta agataatagg
     3601 tgtttcataa atcccttcta ttcatattct agaaatgaag aaagtgggaa gccccactct
     3661 ctgttttatt tgtttaagta aggcaataga tagccatatc ctgctttttc catctcatcc
     3721 ttggccacaa agcgtaaaga agcagaattg atacagtaac ggaggccgcc taactcccgc
     3781 ggtccatctg tgaaaacatg acccaagtga gcactgcctg aacgagaacg aacttcaatt
     3841 cgctccattc catggctcag atccttgtaa taatgaatca actctttgga aatcggacgg
     3901 ctaaaacttg gccaaccaca acctgaagca aacttatcct tggcaaaaaa gagtggctca
     3961 cctgtcgtaa tatctacata aatcccctct tcaaaggttt ggtcataggc attggtaaat
     4021 ggagcctctg tagcagcttc ttgtgtgaca cgataagact cttcagatag actggccttc
     4081 aacacctctt gactaggctt ttcatagttt gctgcatcaa tcaatggctt atcagcatcg
     4141 gtcacatcga tatgacagta acctgaagga ttcttcctga gatagtcttg gtggtagtct
     4201 tcagccagaa tgtagtggcg taattgctcc acttctactg caatctttcg acccagcatg
     4261 cgttcctgct cctgcaccac tgtgtagata gctggcaaat ctgcttcatc ctgataataa
     4321 atcccagttc gatattggcg accacggtca ttcccttgtt gattgataga tagaggatcg
     4381 ataactcgga aataataaag taaaatctct ctgagtgaca cttccttctc atcgtaaatc
     4441 acttggaccg tttctgcatg gtctgtttcc ttgatcaact ggtaattggt cgtttcgact
     4501 tgaccattag cgtagccaac actggtttct agcactccag aaatgcgtga aaaatattcc
     4561 tctaggcccc aaaaacaacc acctgctaga taaatttctg ccatctttat ttctccttta
     4621 tcacgttttt aactaatact cttcgaaaat ctcttcaaac cacgtcagcg tcaccttacc
     4681 gtaggtatgt tactgacttc gtcagttcta tctgcaacct caaaacagtg tcttgagctg
     4741 acttcgtcag ttctatctgc aacctcaaaa cagtgttttg agcagcctgc ggctagcttc
     4801 ctagtttgct ctttgatttt cattgagtat aaacttcttt tcacaaaaag gatatgaaac
     4861 cctctggtca agagtttccc catcctatct tctattcttt attttgcttc tacacggaca
     4921 cctttggtat caactctcaa gtcatgcagt tttcctttga aaggttgctt ttccaattct
     4981 gccttaattg ttggcatctt gtcatgagaa gccagaacca taactgtcgg cccagcacca
     5041 gaaaggtagg ttgcataggc cccattttct ttggtcactt gcttaatcat cgcaaattct
     5101 cttaccaagt cctgacgata gcgctcatgg aagaggtctc cctcgattgc ttgcccagcg
     5161 gtcaccatgt ctcctgccaa caaggcagca accgctacat tggcgataga acttgcagca
     5221 acagcttcct tataagacaa ttttttaggc aagacactac ggctgtcgcg agtacgtaat
     5281 tcatagtttg gaatgtaagc tagaaaatca cactctggaa agtctgctac gatagcagag
     5341 acttgccctt caacagaact tgcaataacg agattaccat aaatggctgg agccacattg
     5401 tcaggatgcc cttcaatctt ggtcgctaac tgcaattttt catggtctga taagttgagt
     5461 tgacccagtt ggttggctag ttcaatccca gcaacgataa ccgagctgga agaacccaaa
     5521 ccgcgcgcca aagggacatc actggtcatt ttcaagcgtc ttggttgcaa gtctggtaca
     5581 atttgcaaag cgattttaag caagagatta cgctcgtcat gtggaatcca tttgccaatc
     5641 tggtgttcaa tcagccactc atctcgttct tcgcagacct caatttgaag atacttggtt
     5701 acagctacac cgaccgagtc aaaacctggc ccgatattgg cactggttgc aggtacaata
     5761 atcttcatct tattctccta gcaccttgaa ggtattcaag aggtcgaatt ctgaaacctt
     5821 cttcaattca gctgagacat tttcaagctg ggctttatta atcttgtgtg tgatgataac
     5881 gacacgcgcc ttgtcaccct ctttgccatc ttgaaggatt tgcttaaagg aaatatcttg
     5941 agcattgaag atttcagcca acttcaagac ctgacctttt gagtctagag ccaagattga
     6001 gaaatagtag tttgctttga catcttcagg atttgccaag accaagtcac ggctatattc
     6061 gttgaagtct ttgccaatag taccatcatt caaacgacga acgatacgga caatatcagc
     6121 tacaacactt gttgcagttg gtttttgacc cgcacctggt ccgtagtaca tagactcacc
     6181 aataccgata gattctacaa agacagcgtt cattacgcca ttcacactag caagtgggtg
     6241 cgctttaggt aggaaggttg gagtcacttc tgcagcaata cctgaagaag tttcctcaat
     6301 agaaccaacc aatttcacta cgtaaccaag ctcttgagct acagctacgt cttctggtgt
     6361 gatattgcgg attcccttgt gggctacatc atcaaaggca atcttcatgc caaaggcaaa
     6421 ttggctcaaa ataaccatct tgtaggctgc atcaatccca tctacgtcat tcgtcggatc
     6481 gctttctgca aatcctagac gttgtgcttc cgcaagagca tcatcgtaag accagccttc
     6541 ttccaccatc ttggtcacca tgaagttgga agttccgttg actactccaa gcacgcgcgt
     6601 aattttatca gaagccaagg aatttgctaa agtacgaaga attggaatcc caccagcaac
     6661 tgctgcttcg tagtaaagtg ctaccttgtt agcttgagcg atttctagca attctgcgcc
     6721 atggacagct aaaaggtcct tgttagcagt aacaacgtgt tttccagctt ccaaggcacg
     6781 agtgataaag gttttagcag gctcaatacg ccccatcaat tccactacga tagtaatatc
     6841 ctggtctgat aaaatatcat ccacattggt tacaaagtta aagtcattcc ctgctgcaag
     6901 caagcgattt ttttcatctt catccttgac caatacctta gcaactttga tatctgaatg
     6961 tgctgattga ttgatttttc ctccattttc ctttaggagg aaaggcacac cacttgcaac
     7021 ggtaccaaat cctagtaaag caattttaac tgtcatcttt atctcctcga aattttctaa
     7081 tatagccatt ataacagaat tttgtgaaaa ttcctattat agtaaatcac tatttcagta
     7141 taaaaagaaa aaacgaatca gacgattcgc tcttcttaaa atctgaaaat agctttccag
     7201 aaaagattag ccgatttttt gcagattgag cactgcatcg tgactcatca agacttgacc
     7261 atactcttgt aagactgagc gactgatatc actatcgtct gcaaactcgc gcatacgggc
     7321 caacagccaa gctggatatg ggcttggatg attttcaata tccactaaaa tggtcaaata
     7381 atagcgctcg ttcattttgt agagttcaga agtttccatt tcaaaagtca ctgtcttggc
     7441 aaaagctacc aagtcagcca acttagcaaa agaaaggatg tagtagatgt aaggttcttt
     7501 cttactctca gcttcttgtt cagcctgctc ttgctcttct tccttggctt caacttgctc
     7561 aagagattga atggcttcga tatcatcctt ggttttgtct gcgatgcttt tttccagggt
     7621 tttgataaat tcatctggag acatttgagc caattcttcc atatctggca aatccgataa
     7681 gtcttcaaaa tctagatttt ggtcaatctt tgacttggtc acaaagacat ctaccttatc
     7741 aggttttgga gtcacacgga agctcaacat gcctgtatcc agaaagctat caggcatctc
     7801 tagctcatcc aagatagcat aaaagaactc ttctgttttt tcttgaggaa cgagaaagtc
     7861 agcaatctcc attccacgat ccatcaaatc ctctaaagac atcgtgattt ttaaagttgt
     7921 atcactaatt tgtttcattt tcattgctag taacctcata ctttcagttc tatctattat
     7981 actagatttt tacgatttta tcaaaagaag gctcctctat acggatagat tttccctagg
     8041 gtctttctat aggagactcc aagagaaaat ttctgcagac agatagaaaa agccttcaaa
     8101 atcggctaag agccgatttt gaagacctta tacatcagaa tacttataat ttaaaggttg
     8161 ctacaccgag gatagaacga tttaagtttc tgagaatttg aagactttgc tcaaatttct
     8221 tataacgagt cactccgtac tcttcaacaa gaaggactgt atctctttcc aaaagagatg
     8281 atacatcctg taaatctaca aaatgcattc cttttaaagc ttcttgactc tgtttcaatt
     8341 tatctaggat agctttattt gagctaacga tggtcaattc ctgtccagta tttttgtatg
     8401 acaaaacatc tgctaggtta gcaattgttg taatctctgt tacaaaatca atttgatact
     8461 gagaaaaatc acctactcta ttgattgttg gattaaagag ataaactaac acatttccca
     8521 tcacaaccaa aatcacacaa accactccaa taacaactaa acgaagaatc agatttttca
     8581 catttaagcc aagcgctgtt tcaccatttg cgttcaattc tttagagttg atggtttcca
     8641 gtttttcaat tttcacattt gcataggcat gtttaaattt ctcaatcaac ccatcaattt
     8701 ttttctctaa caagtcattg gcatctttac ttgatgtcaa aattttcaca ccaacccctg
     8761 catcgtcaat catatagtag acggtcaatt ttttccacca atagtcattc gttgaatttt
     8821 tcaaggttgt ttctgtcgtg tctaattcac tggcaatttt tttcaactca ctgggttcta
     8881 catcattgaa aagataagct ccattcaaat taccatcaat caatttccca taaaaatcac
     8941 tataaccacc aatttgatga ttcaaaatcg ttttggccga ctcttttgga ggagtgattt
     9001 tatagataag ataagttgaa taacttgttg tatctttgac agtgttttta ttcctaactg
     9061 ctttaattgt aaatggtaca gcaatgagag caaataaagc gatgagagct aaaatatttg
     9121 cttttcgctt tttataaaga tttgcaaaca aatcagctac tgaataatgt tcaaacatga
     9181 tttttttctc ctttgtttag tagatactag ttttcctttg taagcatttt tgctataaat
     9241 ataatcacaa gaacaattcc ccagaattgc attgtaaata aattgaagaa actttctgaa
     9301 aagctgcttc ttggcataaa gaatagatta ttcaagatga gtagggataa agcaaatagg
     9361 attgtccttg agcgataggc tacttgcagc atggctataa ataatacgcc gagtaagaaa
     9421 ctaagcagaa agactccaat cataccatag tcggtataca actccatgat ataactactt
     9481 ccgataccat gccctttcag gtattccttg ttcaagacaa gataggatag attgtgggca
     9541 taactattac tatcaatagc tagttccaca ctattggttg tatgttcaaa ggcttttcct
     9601 ccgaaaatgg ctcccaaact cccccttgca aaataatcaa gaacaggacc aaaagtaaaa
     9661 ttacggaaat ctcggtaagg gaggctactg ttaaatagaa aacctcgagc cagaacacca
     9721 aaactagtcc cttgtttata gataaagtca agtaagatat cccagaaacc tgtatgggaa
     9781 acttggacat tatcccgtac ataattgagt actcccatcg ctaacatgag aataggagaa
     9841 cctacaaaaa tcgctaactt ttctttaaac ccaatccatt ttcctttttc agtttgctcc
     9901 cgcataaagt aataaacaaa agcaaataaa atacttaaaa taaagggatt tcgtgtccca
     9961 attgccaaat gaatagtatt agctgcaata aaggagacaa gcactgctgt ggcctgcaat
    10021 ttctttggct tggttgccag atacatacac attgcataga ccgtaaaggt agacaaaatg
    10081 taggtaaaat aaggcagttt actttcaaaa tttgcatagt aggcatagta ggaagtctgc
    10141 aaacgataca agagccgttc aaataaccga atgaaataga aaggataagt tagaagaaaa
    10201 actcctagtg atacaaagcg taaccgcttg atataaacct cttttagaga atttcctata
    10261 tttgctactt ttattttctt cctagctatg aagtaacgag ccagaatgcc tcctgtggtc
    10321 aagcccagaa tcgaaaccat gacaactata aaggcaaaac gataggctat tggatgatag
    10381 gtatccaaag caccatccct aaaataatca atggtcggtc ttgataccag aaatacaaaa
    10441 atggttaaat agaaaataaa atggattaag taatacttga tatcattcca acaagcaatt
    10501 aagctactaa ccaacaagaa caataaagta gaaagtaagc taacattatt attattaaac
    10561 agatacacaa ttccacttac tagcgtcaag gcataactga ctatggtcaa actaaataat
    10621 aatcgtttcc catcaatcac ttggtcaccc ccgttctaat gtaatttttt agatttttca
    10681 atatttttca gtaataagaa tcgatataag gaaatattta tgaatagggc caaagcacta
    10741 attcttctcc ccttacggaa aattggattc ctagaaatag caaaggcatg gccttttaaa
    10801 aaacgatgaa tctgagaata ggcttcaaac tgtttatact gatcatctag caacatctta
    10861 tccagaataa agaagtgggc ataggccaat ctgaaaaaag cgacctcttt caagtcagga
    10921 tagtttttca caacttcatt ataaaacttt tggtagatat caatataggc taaatccttc
    10981 tctgcatagg gtttggtcgt aatactatcc cctctatgga aatagtaata atagggttta
    11041 gtattaacca catacttctt ggccaacttg attaaatcaa aatggtaata ggcatcttcg
    11101 taaatcaacc ccttaggaaa ggatagggca gttgcaatct gtctcttgat tagcttattg
    11161 caaatcgtcc caggtatttt ttcacctatg aggtattcct ttagaaatgt ttgagaatca
    11221 cagacaaaat agtcatcctg attggctgac tgtgggcttt catcattagc atagacattc
    11281 atgacaccac agcttgaaac atccgcatct tcttgaacta attgctcata taagctctga
    11341 atcatttctg gatggatata atcatctgag tcaataaaaa tcagataatc cccgtgagcc
    11401 tgcttcatcc catcatttcg tgcttgcgac aatccttcgt tctttttatg aagcactgac
    11461 accctgtcat cttgttcagc gattgagtca cacaagcgac cactttcatc tgttgcacca
    11521 tcatcaacaa gaataatttc cagattttga tagatctgct tctgaatgga agctatcgat
    11581 ttttctaggt actgcgccac attatagact ggcacaatca cactaattaa tgcagtttcc
    11641 atgctactcc tctaatagtt tttctacttg ttcgatttgt tttgtaattg taaattgttg
    11701 aatgaattgg ctagcctcat cgacatcaaa gtttgaggca gaagtcatgt aattagtaat
    11761 cgcctgagct gcctcttgat tgctctcaat gatttgtcca aatcgtcctt cttgggataa
    11821 ttcctcagcc cctccaacgt ccgtagagat aaaagggagt cccagactca aggcctccac
    11881 atacactcca ggaaaacctt cttgtttaga catagacaag agaactttcg tctgagataa
    11941 atactgataa ggattttttt gataaccaag gaaatgtaca tagtcctcaa tcccatactc
    12001 tttgactcgt tttttcagtt cctcttccat atcaccagcc ccgataaaat agagatgata
    12061 gttttttccc tcttggtgta ataatcgtat cacttccact acacggtcag aacccttatt
    12121 ttcctcaatc cgtccgatag tacagatact ttgaggagca atctcgatat cgatcttctc
    12181 ttgagatttt tctagaatag tctgaaaatc atatccattg tagattatct gtaatttaga
    12241 agcataatct ggataaactt ccttgataga attgctggtc ttttttgaaa tccctacaat
    12301 tgtattcgca gcatccaact ggcttctatg tgattctctt ttagagctat ccttaagaag
    12361 ttcttcaata cttccatgaa tccaagatat cttcttgact tctcttcttt tagagaacaa
    12421 cagtggtgga ttcataatgg taaaagaaac ttcaacatca taatcatctt ttacaagcaa
    12481 acgacgagtc agtcttggaa aataaattct cattctccac aaaaaagctc gtaaccatct
    12541 ggtttggcga taatcttgaa gggattttaa aatgcgtaca tgctttggaa cagattcata
    12601 tcccttgtca aagtgctcca tttcaagaat atcaatatca tacttttcta gatccagatt
    12661 tgaaacaatg gttgatagaa tcttctctgc accacctcca agagaaaaag accacataaa
    12721 aaataagatt tttttcttag ccaccatatt ctcccttgta ttctgtataa gacttatcca
    12781 tatcagcgat gacagcatca tgatgcggta cctgcttgtc tgctggtgga ggcgtcatat
    12841 aatccccaaa agcagttctg agatagacat catagccgat tggaataggc atctctgttc
    12901 cttcaaatgg caagaaaaga ttgtcttcaa aagatgtgat tgggtacttg tttctcatgt
    12961 agccaggacc tgagcataat tctgtaatgc catcacaatc agccaaatca tacttagtca
    13021 tttctttctc agcttttttc cagatgcgat aacggagaga ttttggagtc aaacccagta
    13081 aaatgcgact tccccatttc atgagagcac catgcttttc tggaatagtt tgcgcacaaa
    13141 agagtgaata aatcaaggcc caacgaacct gttttttccg ctcagctgga tttttcggat
    13201 aataatccaa aggcaaaaca tccaaggtca gaccatgtgg caaatctaaa tcctgctgat
    13261 aaggcttgat acaggtggtt tccttgtcac gaatggtaat aaaaagatta cgatcaacaa
    13321 aatccttgtg actctttgac aagaaataac gttcatctgc ataacgaggc cataattctg
    13381 ctaatttctc ataatcttta cgaggcataa aaaagtctag gtcgtcgtcc caaggaataa
    13441 atcccttgtt tcgaagggca ccaatagcgc ctccgccaca gagataacag agcaaatcat
    13501 gttctttaca aaaggccaca aaatattcag ccatctccag actacgagcc tgaattgctt
    13561 ttaaatcagt catattgttc attattcttt ctatcgtatc gtttcattat accacaaaca
    13621 aggggtgaaa atctattgca gactgtaaaa aatcaaagcc tgactgctat ccaaatagct
    13681 atcaaacttt gatttttctg tcttatactc ttcgaaaatc tcttcaaacc acgtcagctt
    13741 caccttgccg taggtatagg taactgactt cgtcagtctt atctacaacc tcaaaactgt
    13801 gtttttagca gcctgcggct agcttcctag tttgcacttt aattttcatt gagtattatc
    13861 ttatctcaag cccatttgag cgagcttggt ttgatatttg ttttgatcaa ccagcaagcc
    13921 caagcctcca taaacatcat aggcatctac ccagtcaccc agttctggaa tcgtcaattt
    13981 ttcaatacca ttttttgctc catccaaaac agataaaccg tttgttagga ggaaagtata
    14041 gggtacgttg gttgaggtca tagcaaaaac ctttccaaga gcttcagaac cagtgaaaag
    14101 tttagtggga tctttaattt gctctaaaat tgctgttaaa acttgttgct gtctttttgt
    14161 acggccgtaa tccgcctcat catcatcacg gaaacgagca taattgagca gggtcgagcc
    14221 attcatctgc tgttttccga ctttaatggt ttgggttgga gactcagtct cggtagcgta
    14281 taaatcatct ccgactgtag cttctgttag gggacgccca ttcaatgttg aaaattgagc
    14341 atcaatcgtc accccatcag ggaaaagcgt gtcaatcgct gtggcaaagg cctggaaatc
    14401 aaccaaggcg tagtacttaa tgtccaagtc aaaattatct ttcaagactt ggcgaaccat
    14461 ttctgcccct ttttgcccct cttgttctcc taactcgtag gctacgttta acttgttatc
    14521 tgtctgtttt ctaccattaa tcacttgact ataaccatct atatagacca aattatcacg
    14581 catgaaactg actagcttca ttttcttatc tgagcccccg acatttaata ccataataga
    14641 gtcagttcgt gtctcaacac tgttctggcc gattcgacca tcagtaccca tgattaaaat
    14701 attaactcca tctctagtgt cctgaccatt aaagacttct acttgagctg cccgggcatc
    14761 agcagttttc tttgcgctag catcttggta accacgcaaa aacatgaata ccatggccaa
    14821 agccacacag accaaaagtg aaaaaatcac cataaaaatt cgtttaagac ggagcttccg
    14881 tcttttcttt tttggaggga aagagagtgc ttgtgatttg gattgtgagc gactccggtt
    14941 cgcatagctt ggtaagtcaa cctgctcttc tctttcttgt tccaagctag agctactatt
    15001 tcccctagca agagttagct tttcttgcaa ataggcaaac tcattttttt ctctctcatt
    15061 gagatagtga atatttttta gcaaataatc ataacgcaac tgctcatgat gacttaaggg
    15121 attttcttta ctcatcttct ctcctttcca tggtctgata ttggataaat aggataggca
    15181 cccagaattt tatactggat tccaatcgct tctaattctt tttgggcaaa gtggaccaag
    15241 tccttatcga tataatccac atcgataatg aaaaagtatt cacccagtgc tgtcttgagt
    15301 ggacgacttt caatttttgt caagtcaatt cctcgccaag caaaggtcga cagggcctta
    15361 taaagtgcac ctggaaggtt gtcaggtaat gtcaaggcca aactcatctt ttcagtttgt
    15421 gcttgcaagg gaatactagg cttttcagct cctagaaccc agaaacgtgt gaaattggct
    15481 tccatttcct gaatatcctc ggcaatcagt tccaatccat attcttcagc agaacttcta
    15541 ggtgcaactg ctgcaaaggg ctggtctgga tgttcggaaa taaaacgggc cgcataagct
    15601 gtactagctg ttacctcgat ttgagcctct ggatattgtt catcgatgaa tttctttcct
    15661 tgagccaagg cctgtggatg tgaaaaaatc ttttcaatct tagtatggcc tggaaccacc
    15721 atcaactgct gatgaatagg ctgaacgatt tctgctactg cttggatgtg agcctgatga
    15781 aaaagatagt ccaaggtttc atgaacacta ccctcaatag aattttcaac tggcaccaca
    15841 gaatagtcca ctaatccttg ctcataagcc ttgatgacat ctgtaatgtt ggcaaaagcc
    15901 tgcaattcct catgaggaaa agctgtctgc acaacgtggt gtgaaaatga tcccttggga
    15961 cctagataag ctattttcat cttagttcct ctataatttc ctctgggctt agcttggtca
    16021 catccaaaac ccgactagcc acttcctcat accaagcctg tctttcttga aaaatagcta
    16081 ctagttcttc cttgctatta tttagaaaaa gcggtcgctg attgtcctta tcagctgcga
    16141 tacgtaggta gagggtttca aaatctgctt tcaggtagat gttatctgta ttagtcttga
    16201 gtaagtcacg atttctctga gaaataacca ctcctcctcc agttgacacg acttggtctg
    16261 tttgtagtaa atcagctagg acttctgatt ctacatgacg aaaggctgct tctccctttt
    16321 cagcgaaaaa attcgcaatg gacataccta ggcgcttctc aatcagagca tccatatcaa
    16381 ggtaattagg gtccaagcct cttgcaatag tcgattttcc agcccccata aaccctaata
    16441 acaccttagc catgaatcaa gctctccaaa tcatcaaaga aactaggata gctggtattg
    16501 atggcttctg cacggtcaag ctccacctct ccatctgcaa ccaagagggc tgcgatagct
    16561 gtcatcatgc cgatacggtg gtcaccaaac gtattgactc tagcaccgtg aagagctgat
    16621 ttccctttga taatcatccc atctgccgta ggagtaatat ctgctcccat actatttaag
    16681 gcgtctgcca caacctgaat acggtctgtt tccttgacct tgagctcctc agcatccttg
    16741 ataactgtta caccttgggc ttgagtcgca agcagggcaa taatgggcaa ttcatcaatc
    16801 aatcgtggaa tcaaagcgcc accaatctct gttcctttca agtcagaaga ctcaacaatc
    16861 aaggtagcag atttagcgac tggatcgatt tcagttattt ccaattttcc acccatggca
    16921 cgaatgacat caataatacc ggtgcgagtt tcgttgatcc ccacattctg cagcactaga
    16981 cgagaatttg gagcaatcaa acctgcgact aaccaaaagg ctgcactgga aatatctcct
    17041 ggtacgacca ccttctgtcc tgtcaatttt tgtggcccct ggactgtgat tttcttacca
    17101 tccacactta aatgaccacc aaattgttgc aacatatctt cagtatgatt acgggtgtac
    17161 tctttttcga taataactga ctccccctta gcttgtaagg ctgcaaacat caaggctgac
    17221 ttgacttggg cagaggcaat tggcaactca taatgaatag gtcttaggtt tttcgtccct
    17281 tttaagcgaa ggggaggcaa gtctcgttca gtttgccctg aaatgctgac gcccattttt
    17341 ttcagtggaa gggtcacacg gtccatagga cgtttggaaa gactatcatc tccaaacatc
    17401 tctacttcga aatctgcacc agcaaggaca cctgaaatca ggcgaatcga ggtgccagaa
    17461 tttcccatat taagggcatt ttgtggcgct tttaagccag ccatgcctac accttgaatg
    17521 gtaataaccc catctttatc ctcaatttca acaccaaggt cacgaaaaac ctgcatggtc
    17581 gaaagaacgt cttcacctcg cagaatatca taaaccttgg tctcaccctc agccaaactt
    17641 ccaaagataa tggaacggtg gctgatagac ttgtcacctg ggacgcggat actaccatgt
    17701 aaatggcgaa tgtttgtttt tagtttcata ctggacctca tacttgcaat acttttacct
    17761 attttatcat aaaaagtcag aaattcctta aaaattcctg actttaggat cgttcttttc
    17821 ttatttcagc aattctgaaa ctggttcaaa aacaattttt tcaatgtcag aaaggtaaat
    17881 ggccaattgt tgttgcttgg taaagaattc tgacaagagg ctatttcctt gaatctgttt
    17941 accaaagcct tccatcttag cttggaagga cgcatctggc atttgacctg tctgtgctag
    18001 tttttgaatt tcctcttgaa aggcaagata ttctgtaaag attttgcttg cctcagcatc
    18061 tgctgcaatc gcatctttag ctgctttaac agccttgtat tctggtaatc cgcgtagacc
    18121 gcgactgagt tcgtttgcac tatcgtaaat atttgacatg ttcttctcct tatttgatga
    18181 cgactgtata gtcggtattt tctgttatga gatgctcagc tctttccaag tcttgagcat
    18241 ttttaaatga aatttgtagg attccgtgaa tatcctcacg attttcctcg ttgatgtgga
    18301 tgttaaccaa ggaagttcca cgtagcagtt ccaaaatccg caggatgaca tcttcttcat
    18361 caggaacgtc aacatagagg tcgtaagagc tatccacacc accacgctta tggatttcca
    18421 tggtctggcg ttgttcacgc gcttggttaa aaaagttcca aatttgctct tcatctccct
    18481 tactaatggc ctgaccaatc acttccaaac gttccttgaa atcctcaatt ctatccagaa
    18541 tggtctcgct attggacaag agaatggagg tccacattcc tggctcgctt tccgcaattc
    18601 gggtcatatc tcgaaaacca cctgccgcaa agcgccttgc catctcatgc tcttgagcat
    18661 agaccgcagt ctgctccatg agactagaag ccaaaatatg aggaaaatgg ctaatctgag
    18721 aagtgacacg atcatgctcc ttggcatcaa tctcgataaa acgagcatga agacctgaaa
    18781 gcagatcctt catttcctta agcgtgtcct gacttgtcag gcttgaaggt gtaaagatat
    18841 aataggcatt ttcaaaaaga ttgacatctg ccgaagcagc ccctgtcttg tgactaccag
    18901 ccatgggatg ggccccgaca aagcgaacag acttgccagc caaatactgc tccgccgcat
    18961 ccacaatggt tgacttggtc gaaccagcat ctgaaataat aacgccttct cgcaaatcca
    19021 aattggccaa ctccttaatg aaagcaatag tttgtttgat tggcaagctg aggataatga
    19081 catctgccaa aggagcaaaa ctagcaaaat catccgttgc acggtcaatc ataccttctt
    19141 tcaaggcgat atctctcgaa gcttgactac gattataacc taaaatttca taatctggat
    19201 gatcgcgttt gataccaagt gccatagagg ctccaatcaa cccaagacct gcgatataga
    19261 ttgtttttgc cataggaact ccttaatagt tctttgtata gtctcggtgt ttggctaccg
    19321 cttcttttag ttcctcaaga ttatctgatg agaatttttc gaggatttct tgcgccagaa
    19381 ccgttgctac aactgcttcc atgaccattc ctgcagctgg aagagcagtc ggatcacttc
    19441 tctccacggt tgccttgtaa ggttcgtggg tttcgatatc cacactcata agaggtttat
    19501 aaagagtagg aatgggtttc atgaccccac gaacaacgat gggttgccca ttagtcatac
    19561 caccttcaaa accacctaga ttattggtac ggcgagtata accgtcttct ttagaccaga
    19621 gaatttcatc cataacttgg ctgcctttac gataaccagc ctcaaagcca agaccaaatt
    19681 ccaccccttt aaaggcattg atagagacaa cagcttgagc caatcttgca tccaattttc
    19741 tatcccattg gacataggaa ccaagaccaa ctggaacgcc tccgacgact gtctccacaa
    19801 ccccaccgat ggtatcacca tcacgtttga tttggtcaat atagtccttg atttcctgtt
    19861 ctcgttcttg gttgacaata gaaacttcag actgggcagc tctttgctta atttcagcga
    19921 ctgtcagatt ttcaggaaca tctatttcct taccaccaaa gaccacgaca tggttggcaa
    19981 tctccatatc cagctcagcc aagaggcgtt tggctactgc accaactgcc acccgcatgg
    20041 tggtttcacg agctgatgaa cgctccaaag aatttcgcaa atcatcaaaa cggtacttaa
    20101 tccccccaac caaatcggca tgacctgggc gaggatgagt aattttccgc ttgcttttaa
    20161 ggcggtcttc aatgtcctcc gcagacatga tgtccagcca tttctggtgg tccttattga
    20221 tgacatccat agtaataggc gcccctgtcg tcttcccgtg gcgaacaccc gaagtaaaga
    20281 caacctggtc actctcaatc ttcatacgac caccacgacc gtagccaccc tgacggcgtc
    20341 taagatcctc attgatatcc tcagctgtca atggaagtcc agctggaatt ccctcaataa
    20401 tagctgttag acgggggccg tgtgattctc ctgcagttaa atatctcata cactctcctt
    20461 attttaccaa gtagtctttc atctcttcca gagaaactgg gtgaatggtc gctgaaccaa
    20521 gctctggcac caagaccaat ttcaaggtgt taccacgcgc tttcttgtca tgagtaagag
    20581 cctgataaag cttgtcaact ttccaatttt catagtcaac aggcaagccg aatttctgac
    20641 acatctctgt gatagattgg gtaatgccag ctggcatgag gcctttttcc tcagcaacct
    20701 tggaaatctg taccattccc atggcaacag cctctccatg catgaccttg ccataaccgg
    20761 cagtcgcttc gatggcatgg ccaatagtgt ggccaaaatt gaggtaaaga cgaataccat
    20821 tgtccaactc atcttcaacc accatcttgc gcttcacctg acaagaatgt tcaatcaagg
    20881 tctctgcatg ttccaaaata ctctcaacag aaccattcag tcccgtcaag agagcccaca
    20941 gttctggatc ctcaatcaag ccatacttga taacttcacc catcccttca atcaactctc
    21001 tttttccgag ggtttcaaga acaagtggat caatcagaac cccatctggt tgggcaaagg
    21061 tccccaccat atttttagca aatggtgtgt taacgcctgt ctttccaccg atagaagaat
    21121 caacctgagc tgtcaaacta gtcggaatct gaacaaagtg aataccccgc atataggtag
    21181 aggctacaaa gccagccagg tccccaacaa cgccaccacc aagagcaacg attccatcgc
    21241 tacgagtcag accttgcttg actagaaatt catagacttt ctgaacagta gttaaattct
    21301 ttctttcttc accttctaag aaatcaaaaa cagctacctg aaaaccagca tcttctaggc
    21361 tgagcttgac cttctctgca tagagagagg ctacatggtt atctgtcaca atgactacct
    21421 tttgcggttg ccagagttct cgcaaccact gaccagcctg ggccatacaa cctttttcaa
    21481 tctgaatatc ataaggatga tgaggaatat cgattctgat tttcatagga gagtctccct
    21541 ttctttattg atatttttct gttaaagact gccaaatctc ttctgtcggc attttcttgc
    21601 ctgtccacag ttgaaaagct tctgcagctt gatagagcaa cattcccaga ccattgactg
    21661 ctggattgcc ctgacttcta gcccatttca aaaacggtgt ttcaaagggt tggtatatga
    21721 tatctgcaac taagagagtt tctggtaaga ctatgttttc aggaacagga gaggattggc
    21781 catccatgcc cacactggtg gcattaacta gcaaatccga ctcggcaatc cttgcttgca
    21841 gttcagaaac atattctaaa gcacacaaat ccactttaaa gcctgtctgc tcctgtaact
    21901 tgtctaggta aggtcttgtt ttttccatag aaacggaacg aacaaagacc gaaatctgac
    21961 tgacgccatc caaaatagcc tgtgccaaga ttgatttagc cgcaccacct gcacccagca
    22021 gggtcatctt tttacctgaa attgtaaaag aaggcaagca cttaaaaaat cccttgccat
    22081 ctgtattata tccaattaaa ttgccattct cattgacaac cgtattaacc gcaccaatca
    22141 agcgcgcttc atcgctcagc tcatccaaat aaggaatcac ctgctcctta tagggcatgg
    22201 acagattgat gccaaacatc tggtagcgac gaatattggc cactgtttct accaagtcac
    22261 tcgcttcaat ctcccaagcc acataagcac cgttggtagc tgtcgcctca aaggctctat
    22321 tgtggatgaa gggagaaata gaatgcttaa taggattggc aacaactgca gctaaacgtg
    22381 tatagccatc aagcttcatc caaaatctcc ctgatttttt tcatgctagc tagagaaatc
    22441 tgcccagggg cactaacctc atccagactg gcaaaagacc aactcgaacc agtcacatcc
    22501 gcagtgatac gagagacctt gcccacctta cccatagaaa tggtcacata ttcctgttca
    22561 ggattgagcg ttttaaagcc tcgtgtatag ttcatcaagt ctaagacatc ctgctccgtg
    22621 tgagccatca ccgcaacctt aacaagtttt ggatttagga tcgtcaactc tgacaagatt
    22681 tccatcatgt tctcaggtgt ttcttggaaa ttatggtaac tcaaaacaag atttgggaag
    22741 tccagcattt cctcaaaaac atccttgtag ctatagtact caaaatcaat atagtctggt
    22801 tgatagagtt gcgcaacttc cttgattaga tggatatact cttctggaga aaggtcgatt
    22861 tctccacctt cggagcgagt tcgtagcgtg aaaaccaact cacggcctgc gaatttttca
    22921 aaaatggctg gagctacctg caaaatcgct tctttaggca gatagtcggc acgccattca
    22981 atgatgtcgg catccaggta cctcgtggca tccagagcct gagcctcctc taaacttctt
    23041 ggcattactg aaacgattaa tttcatttac taaccttcat actaatcacc ttgaggtaat
    23101 tactactttc atctttttta ttataggcaa aatctgctgg aagaccatat ttgtttaaaa
    23161 tctggtaact tcttcctgca aagcctttat caatttgttc tgtaaatttc tgacgggaaa
    23221 cattggcagc attggtactg gcaatgataa tccctcccgg atttaaaatc tcaagactct
    23281 gggaaatcaa cttgtgataa tccttggcca cagagaaagt ttgtttttta ttccgagcaa
    23341 agctaggcgg atctaggaca atcacatcgt aggtcaagtt tttgcgtttg gcatatttga
    23401 aatactcaaa gacatccatg actataaaac gacgctcgtc tgtgctgagc ccatttgcct
    23461 gaaaatgcgc ttgagacaat tctcgtgaac gtttggctag atcaacagaa gttgtctggc
    23521 tagctcctcc catggccgca gctactgaaa aagccgctgt gtaggaaaac atattgagca
    23581 aggatttacc catagccaag ccgtcaacta aactaccgcg aacctcatgc tggtctagga
    23641 aaattcctgt catcaagcca tcattcataa agacttgata caggacacca ttttctaaaa
    23701 cagtgaaaaa gtcaggtgct tcttgaccat aaacatgggc agattcatag tccaaaccct
    23761 taaagcggat tttctcataa gcccctaaaa cctcagggaa aacctgtcta aaggcttctg
    23821 atatagtctg acgaatctga taaacataag agttatacca agaaaagacg gcgtagtcgc
    23881 cataaaggtc cactgtcaga cccccaaagc catctccctc ttgattaaag agacgaaagg
    23941 cagttgtcaa atcatcttga tagtaggcgt ttctcttttc tttggctttt ctaaacaacg
    24001 tttcaaagaa agcttgattg aaggccacct tgtctttgct gacaaaccag cccaagccct
    24061 tgttttgctg agaaaggtag gcagtcccaa gaaagtttcc ttcctgaccc tgcacctcta
    24121 cttcctgatc cttaagattg acattctcaa gatcactggc ttctagtaaa actagcccct
    24181 tagcaagctt cttttcaacc cttttgctga ctcttattct attcataact accattatat
    24241 caaactttta gacaattctc aaaaaagaaa ctacccttgc ttttttactc ttcttttaaa
    24301 aaatggtata ctagacttcc tgcaaaacta ggaagtaaat gtgtaagaat cacaataaaa
    24361 aatgctcttc cgtcttggag gagcatttct ttttatcaac gaaaatcaaa taacaaacta
    24421 tgaaactagc ctcaggttaa ctgtgagatt ataggtagag aggttgaatc agcaatatgt
    24481 gtctgtcaaa tttagtgaca aaggtagtag aagaaagatg aagaaataaa tctgcttcag
    24541 tagatatctg gaaaatttga ttttatagaa aagccttttg ttacaaactc aatatactat
    24601 caataaataa tattatagaa gcaacaataa ttaaaatttc acctatttgc atcattctat
    24661 ttcgaactct aaatatatgt tctatcaaaa atacttggat cacacacatt ataggaatta
    24721 acgtttttga aattgaaaaa tatcccaata aataaactat aaacaacaat aatagaacta
    24781 tattatattt cttattcaaa acattcctcc ctatattttt gattaccaat cttaatcatt
    24841 tacaactaca ttctaacaaa ctataaaagc gtttgtcgaa ttgaatttat caagcaagca
    24901 accaactatc tcatcttttt tctatttctg ccaatatgcg tgacaggtaa taatgatagc
    24961 caaaaatagc aagagcaagc aagacgataa gagctcctac tcccaagttg atggcaagga
    25021 taggggagag agactgaacc aagaatatgc tcccaattac aagggccatc aggattgcac
    25081 tataaataaa caataaaact atggcgacta tgccatttga acgattcacc aggtccgtaa
    25141 tgctactcca attggttgac agatttttaa cgtccttaaa gtaatggtgg caagaaagga
    25201 tgacactggc aatgatccag actacaagaa ggtaaatcat cgaaaggatg gacaagccta
    25261 gatatagaga aagaccgagc aaagtcagaa ctggtaaaaa ggactgaaca gcaaagataa
    25321 tccaaaattt cactttcaca taacgagcaa agtcaaaggg taaactcttc agaaaatcaa
    25381 cattttccct ctccaaggac aaggcaattg aatgcaggct ggtgatattg ttattgacaa
    25441 ctgctataaa cactcctaca aaaaacaagg gtaaccagta tggaggatga atgtctggaa
    25501 ctatctgaga atctcggatt ttggaaatca gactgatcat catgagataa ggaaggaaag
    25561 cacttgtaaa aagcactgta atcacaccag tcccctatcc caagagggtg aggtggtagc
    25621 gtaaaaccat acggaaaaag ccctttttag tagttgaaat cctctccttg ctgcgacgtt
    25681 cttttttgac cttctcctca ctgttaagca ggatcacgtc ataaaaacga ggaaggacct
    25741 tctttttggt cagataaagc aggaagagag ttagtcctat ccaagcgagc agacccacta
    25801 aggcttctgt cgaaaaaggc tccactgcta ttttgtaaaa gatatgaaga ggataaagga
    25861 gaaatggaat gtctctaact ttgtcaacaa tacttccaaa agtcgactga agaaagaaga
    25921 taaatattaa aggtatgaga actcctatcc caatcatcac attcgaaaaa atagactgat
    25981 actttctgaa gaccctagtc tgagccaaga aatgtactgc cactaccgtc actaaagtaa
    26041 cagagacaaa taataaggtc aaggacagta gcatcaaagg caaacccagc caaagagaag
    26101 gagctagact aatatagagg gctagaaaat aagctaggat tggtacaatt ccagttagag
    26161 ctggcaagag gacagacaga cctttggcca ttataatctc tgattcttta aaggcatagg
    26221 gcctatagga ggccaggtcc ttactctcat aaaagacatt gtaaaaggca gtgaaagaag
    26281 ttgaaaaggc cagcaccagt aaaatagcaa ccatcgagct aaaataaata ggtatttcct
    26341 caaaaggaaa atgaatggct atattactaa aacagatgag catcaagaga ctggaaaaaa
    26401 tgtaagaact taggactcta gcagaaacat ttactttttt cccaggattc ttagcctgct
    26461 tctttcgtag attagccaga ttagcttctt gagatgaata gaggatattg atatcaacta
    26521 attttttaat gaccttgaga cgcatccgca acctcctctt ttctaccagc aagactaagg
    26581 tagatacttt ccaaaaactg gtctgggtgg tctttcctca agttctctac actaccacaa
    26641 tagatcaaat gccccttttt caaaatagca atccgatcac agacttgctc ggctacctca
    26701 aggacatgag ttgaaaacaa gaccgtcttg cctttttgtg catgttcctt catcatttgc
    26761 ttcaaatcaa aggcggcctg gggatccaaa ccagtcaagg gttcgtccaa aacccaaata
    26821 tcaggagcag acaagagtgc cccaatgaca aagactttct gacgcattcc gtgagaaaga
    26881 gtttcaataa cctgatagcg gttttcagca aaatcaaaaa cgttcaatag cctagctaga
    26941 ctagtctcca agtcagagct agtcagatca taggatgaag cgatcaattc ccaaaattca
    27001 ttggccgtta agcgtaaaaa taagtcaggc gagtctgcta cgtagccaat ctttcgtttg
    27061 atcgccaagc gattttccga taactcctga ccgtctacca aaatacgacc actgctgggt
    27121 gaaatgatac tgactaggga ttttatggtg gtcgattttc cagccccatt atgaccaatc
    27181 aagcccataa tctctccatt ttcaatctgc aaattgagat tactcaaggc ctctttatca
    27241 ccatacaact tactaacatt ttgaaattca atcatgggac aactcctatc tttatctgta
    27301 ttatacatcg ctatactagc actttgatac aaaaaaatca atactttatt ttaagtagtt
    27361 aaaatagttt ccttctatct tacgcaaacg tttgcgccga gagatacttt atgatagaat
    27421 aaagaacaag attgacaagt aagaggaaac atcatgcaaa atcaaacact catgcaatac
    27481 tttgaatggt atctgcccca cgacggtcaa cactggacgc gtctggctga aaatgctcca
    27541 cacctagctc atctggggat cagtcacgtc tggatgccac cagccttcaa ggcaaccaac
    27601 gaaaaagatg tcggctatgg ggtctatgac ttatttgact taggagagtt caaccaaaaa
    27661 gggactgtcc gcaccaagta tggtttcaaa gaagactatc ttcaagccat tcaagccctt
    27721 aaagaacagg gaattcaacc tatggccgat gtagttctca accacaaggc tgctgccgat
    27781 aacagggaag cctttcaggt tatcgaagtt gatcctgtag accgtacagt tgaacttgga
    27841 gaacccttca ccatcaatgg ctggactagt tttaccttcg atggtcgcca agatacctat
    27901 aatggcttcc actggcattg gtaccacttc accggtacag actacgatgc caaacgcagt
    27961 aaatctggga tttatctgat ccaaggggac aacaagggct gggctaacga ggaattggtc
    28021 gataacgaaa acggaaacta cgactacctc atgtatgccg acctagactt taaacatcct
    28081 gaagtcatcc aaaacatcta tgactgggct gattggttca tggaaacgac tggtgtagct
    28141 ggtttccgtt tggatgccgt taagcatatt gactctttct ttatgcgcaa cttcatccgc
    28201 gatatgaagg aaaaatacgg tgacgatttc tatgtttttg gtgaattttg gaacccagac
    28261 aaggaagcca atctggacta tctcgaaaaa acggaagaac actttgacct tgtcgatgtt
    28321 cgtctccacc agaatctctt tgaagccagt caagctggcg caaactatga ccttcgtggc
    28381 attttcacag atagcctggt tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac
    28441 cacgataccc aacgaggaca agcccttgag tctaccgttg aagaatggtt caagccagca
    28501 gcctatgccc tcattttgtt acgccaagac ggccttccat gtgtctttta cggagactac
    28561 tatgggattt caggccagta tgctcaagaa gatttcaaag aaatccttga ccgcctccta
    28621 gccatccgaa aagacttggc ctatggagaa caaaatgact actttgacca tgctaactgt
    28681 atcggttggg tacgttcagg tgctgaaaat caatccccaa tcgcagtcct tatctcaaat
    28741 gaccaagaaa acagcaagtc aatgtttgtc ggtcaagaat ggactaatca aacctttgta
    28801 gatttacttg gtaaccacca aggtcaagtt acaattgatg aggaaggtta tggacaattc
    28861 cctgtctcag ctagatccgt aagtgtctgg gcagtcaata ccatctaata gctcataata
    28921 accaagctag gtccaagcgg atttggcttt tttgtattca caaaaagacc tacccaaatg
    28981 gatagatctt tacttgatta caatttacct gctactgcat ccaacaattc ttggatctta
    29041 gcttggttgc ttcctcctgc catggccatg tctggtttac caccaccacg tccatcgatg
    29101 attggtgcta attctttgac aaggtttcct gcatgaaggt cttttgtctt gcttgctaca
    29161 aggacattga ctttgtcacc gatagcggca actaggacaa gaagatcaga gtagtctttt
    29221 tgtttccagt tatctgcaaa agtacgaagg gcaccggcat cggatacaga cacttgacta
    29281 gcaatgtaac gatgaccgtt gacttcctta gcatctttga agatatcgcc tgcggctgca
    29341 gctgcggctt tttctttcaa ctcagcattt tctttttgaa gttgacgaag ttgttcttga
    29401 agtccttcta ccttgtgagg tacttccttg acttgaggtg ctttcaaggt tgctgcgaca
    29461 gctttaagag catcctcttg ttcacgatag gcttcaaagg cttccttacc agtcactgcc
    29521 aagatacggc gagttcctga accgattcct tcttctttga caattttgaa gagaccaatc
    29581 tcagaagtgt tgccaacatg agtaccacca caaagttcaa tagagtagtc accgatagtc
    29641 acgacacgaa cttccttgcc gtatttctca ccaaagaggg ccatagctcc catttcttta
    29701 gcagtgtcaa tatccgtttc aactgtcttc acttcaagtg cttcccaaat tttctcgtta
    29761 acttgctgtt caatcgcacg aagttcctca gcagttactg cttggaagtg ggtaaagtca
    29821 aagcgaagga attcaacttc gttaagagat cctgcctgtg ttgcgtggtt tccaaggata
    29881 ttgtgaaggg cagcgtgaag caaatgagtc gcagtgtggt ttttcatgac acggtgacgg
    29941 cgattgctat caattgccaa ggtatattct tggttcaagg caagcggtgc aaggacttca
    30001 actgtatgaa gggcttgacc atttggggct ttctgaacat tggtcacagt agccacaatc
    30061 ttacctgact catccaagat ttgtccgtag tcagctacct gtccacccat ttcagcataa
    30121 aatgacgttt ccgcaaagat aagagaggca gttccttctg aaacagctcc tacttctgca
    30181 ttgtcagcaa cgatagctac caatttagaa gacaattggc tagtattgta gttgaagaca
    30241 ctttctacag tgatgttttg aagagtttca ttttgcatac ccattgagcc acccttgaca
    30301 gctgacgcac gcgcgcgttc ttgttgttct ttcatggctg cttcaaaacc ttcacggtct
    30361 acagtcatac cagcctcttc agcaatttct tcagtcaatt caactgggaa cccataagta
    30421 tcatagagtt tgaagacatc tgaaccagcg ataacagatt gacctttttc tttcaagtct
    30481 gctacaatgc cttgggcaaa gtgttgacct gagtgaaggg tacgggcaaa tgattcttct
    30541 tcgctcttaa cgattttctc aataaagtca cgtttctcaa gcacttctgg gtagtagctt
    30601 tccatgattt ttccaacagt tggaaccaat ttgtaaagga aaggctcgtt gattcccaat
    30661 ttttgaccat gcatagaagc acgacggaga agacgacgaa gaacataacc acgaccttca
    30721 tttcctggaa gggcaccatc accgatggca aatgaaagtg aacggatgtg gtcagcgatg
    30781 accttgaagc tcatgttgtc gccatcttgg tcataaacct taccagacaa tttctcgact
    30841 tcacggataa tcggcatgaa gaggtccgtt tcaaagttgg tcttagcccc ttggataacg
    30901 gccaccaaac gctccaaacc agcgcccgta tcaatgttct tatgtggcaa ttccttgtat
    30961 tcgctacgag gaacagcagg gtctgcgtta aattgtgaca aaacgatgtt ccagatttca
    31021 atataacggt cgttttcaat atcttctgca agcaggcgaa gaccgatatt ttctgggtca
    31081 aaggcttccc cacggtcaaa gaagatttct gtatctggtc cagaaggtcc cgcaccgatt
    31141 tcccagaagt tgtcctcaat tggaatcaag tgacttggat ccactcccac ttcaatccag
    31201 cggttgtaag aatctttatc gtctggatag taggtcatgt aaagtttttc agcagggaaa
    31261 tcaaaccatt cagggcttat caaaagctca taagcccaag tgatagcttc gtcacggaag
    31321 taatccccga tagagaagtt ccccaacatt tcaaacatgg tatggtgacg cgcagtcttc
    31381 cctacgtttt cgatgtcgtt ggtacggata gccttttggg cattggtaat acgtggattt
    31441 tcagggataa tggtcccgtc aaagtatttc ttaagggttg ctaccccaga gttgatccac
    31501 aaaagagttg ggtcatttac aggaaccaaa cttactgatg gttctactga gtgacctttg
    31561 gtcgcccaga aatcaagcca catttggcgt acttgtgcac tagatagttg tttcatattg
    31621 tctccttatt tacttgttta atgtgattgg ctttccagca tttccacata gtcaatcgcg
    31681 acacagaggg aaatgactag gtctgcataa gcgtcttcaa gaaccgttac ggtataggta
    31741 gaggtcagat ggaagagttc cttcttaatt tccgcaatca actgatcgcg atcatccagc
    31801 aatttgaaat tcaaatccca gatattgccc tcgatacgaa gacctagatt atcaaactca
    31861 tacttatctc gccaaaaggt caacttctta cgaatgacaa aactcgagcc atccagaagc
    31921 tgaatctcaa aacgaggaag caaggtcaag atttctttac tgatctgact gacttgttca
    31981 ccagccgcat catagatggt aaaagttttg ggaatcttaa aaaatgatcc ctccacctga
    32041 taggcaattt ctcccctgtc atccttgata gcgaagcgtt cgcctccaag acgaaacttt
    32101 tgtttgacaa gaaatgtttt catcaacacc tccaaaaatc aaaagacaag ctcatatcac
    32161 gaagggcgaa aaaccgcggt accaccttca ttcaatgaac ttgtcattct cttgttctta
    32221 tgcaattgta tgattgagta gcatgacttc ctagcttaga tggctcgcag caccgccatt
    32281 tctctggact aagacaagtg aaaatcaatt ctcaactttc ttattatatc gtttttttaa
    32341 tcttgcgtca actggaaatg atctccgttg aattagacca attccctaca tctctgatta
    32401 cttttccagg atatattttt tcttactgcc gtttttcttt ttatcccaaa ttttcatatt
    32461 actaaacaca gctactagaa tatttccaaa tataaaggtg cctatcaccc aatatatgga
    32521 ctcagttgtt aggtattgtc gatccaagcc atcctttaaa tggaatagta tagcagtttg
    32581 gttaacaatc ataaaggttg gccagaaact tttgttgaaa aaagtagaca ttttcattat
    32641 ttgttgccgc tttctgtaag gttaatactc aataaaaatc aaaaagcaaa ctaggaagct
    32701 agcctcaagc tgtacttgag tacggcaagg caacgctgac gtggtttgaa gagtataggc
    32761 ttagtatact actaggcaag caaataagca aataaacaac tagaatagaa aaagataggg
    32821 ctctaaaaac tgacttctat tccttaaaaa cgaaccagct tgactgattc gtcttcttac
    32881 gtttatctcc tacttccgat acattttaaa ctgtaggaag aggtcgctat atttccctgt
    32941 ccatttatgg tcaaatttct cataaatttc taggtgtttc atggtttcaa catcgggata
    33001 gaaggcctta tcttcctttg tttcctctgg gagcaattcc ttcgctggta tgtttggtgt
    33061 tgaatagccg acatactccg cattttggag agcattttca ggtttcaaca taaagttgat
    33121 aaaggcatag gctgagtctt ggtttttaac tgttttggga atgaccatat tgtcaaacca
    33181 aagattgctg gcctctgtcg gtaccacata acgtagattt tcattttttt ctaacatttg
    33241 gctggcttca ccagagaagg tcacgccgat tgcaacgtta ttctgaatca tatagccctt
    33301 catctcgtcc gcaacgatag ccttgatatt tggagtcagt ttgtagagct tatccactgt
    33361 ctcttccaac tgctgcagat ccttggagtt gaggctgtag ccgagggaat tgagtcctag
    33421 tcccagcacc tcacgcgccc catcaaagag catgatagaa ttcttatact ccggcttcca
    33481 aaggtcatcc caatgctcag gcgcttcatc taccatggtt tcgttgtaga caattcctaa
    33541 ggttccccag aagtaaggga tggagaattt attacctggg tcaaaggact ggttgagaaa
    33601 ctctggtccg atattttcga ttccttcaat ttttgaataa tcaagcggaa ccaagaggtc
    33661 ttcgtccttc atcttgttaa tcatgtattc acttggaatg gcaatatcgt aggtcgttcc
    33721 accctgcttt atcttagtgt acatggcttc gttggagtca aaagtctcgt actgaacttg
    33781 aattcctgtt tcttctgtaa actgagtcaa gagttcagga tcgatatagt ctccccagtt
    33841 atagataacc aatttttgac tatctcgact attgatttta ctatctaaat gagtcgcaat
    33901 tccccacaag acaaggataa tcgctgcaat tcctgctaaa aatgaataga tttttttcat
    33961 gcttgctcct ccttctcacg agagataaag taataaccta caactaggat aatactaaag
    34021 agaaagacta gagcagacag ggcattgatt tctaaggaaa tccccttgcg agcacgagag
    34081 taaatctcga ctgatagggt tgaaaagcca tttcctgtta caaagaaggt cacggcaaag
    34141 tcatctaacg aataggtgaa ggccatgaaa taaccagtaa tgatagacgg agtcaggtaa
    34201 ggaagcatga tttccttgaa catctgaaat tgactagctc ccaagtcata ggccgcatga
    34261 atcatgtcgc cattcatttc cttgagtcga ggcaagacca tcaagaccac gataggaatg
    34321 gagaaggcca cgtgactaga tagaacggtc aaaaagccaa gtgaaaactt gagttgggta
    34381 aagagaatca agaagctagc accaatcata acgtcaggcg caaccatgag gatattattg
    34441 agtgatagaa aggcttcttg gtatttctta cgagactggt agatgtaaat ggcaccaaaa
    34501 gtcccgataa tggtcgctat caaggctgat aggaaggcca agaaaaatgt ctgagccaaa
    34561 atcagcatga gtctcccatc tccaaacatg gtttcaaagt gagtccagct aaaacctgta
    34621 aagctattca tatcatcacc agcattaaag gcatagccaa tcaagtaaaa gataggcagg
    34681 tagaggacca gaaagaccag tcccagataa aggttggcaa attttttcat cgttctctcc
    34741 tttccttagt cacccacatg gtgatgaaca tggtcaggat gagaatcaca ccgatggttg
    34801 aacccatacc atagttgtca ttggttagaa aattctgctc aatagccgtc cccaaggtga
    34861 taacgcggtt cccaccaatc aaacgggtca gcatgaagag actcaaactt gggataaaga
    34921 ccgactgaac cccacttcgc acaccgttca tagatagagg gaagatgaca tgacggaagg
    34981 tctcccactt agttgcacca aggtcataac tagcattgat gagattatta tccatgtcgt
    35041 ctaagacatt gaaaatcggc aaaatcataa aggggagctc gatgtagctt gcgacaaaga
    35101 taaaggaaaa atcggtaaaa agcaactgtt gtgaaccaat tccgataaat tccaagaatt
    35161 ggttaataga gccattttga ccaaaaatcc cgataaaagc ataggcttta aggagcaaat
    35221 tgatccaggt aggaaggata atcagcatga gccagagttg acggtgcttg agacgggtca
    35281 aaaagagggc tgttggatag ctgataagca aggtcacaaa ggtcacaatc cctgcataaa
    35341 gcactgagtt gaaactcatt ttgaggtagg tcaagttttg tgacgcaaag taagatttat
    35401 aattttctaa actaaactgg ccttcaatgt tgaaaaagga ttgtccgaaa atcaagacca
    35461 agggtgccaa tacaaagagg gcaatccaaa gcatgtaggg cactacaaag agtttagagc
    35521 ttgttttctt catctctttc ctcctcgatc gcattgatca gacctgcttc ttgctcttcg
    35581 atttccacgt attcttcgat acgagcatcg aactcttctt cggtttcatt gagacgcatg
    35641 atgtggatgt cttctgattc aaagtccaga ccgatttcct cacccacgat ggccttacgg
    35701 gttgagtgga tcatccattc atttccaagt tcgtcatagg cgataatttc ataatgaact
    35761 ccacggaaga gctgggtgtc gaccttaact tggagtttgc cttcttcagg aagggtaatg
    35821 cgcaagtcct ctggacgaat aacgacctca acaggttcat ttggcttcat cccaccatca
    35881 accgcttcaa agcgtttgcc gttaaattcg accaagtagt cctcaatcat ggtacctggc
    35941 aagatgtttg actccccgat aaaggtggca acaaagtggt tgattggctc atcgtagatg
    36001 tccacagggg ttccagactg gacaatctcg ccatcattca taacgaaaat ccagtcactc
    36061 atggcaagag cttcttcctg atcgtgagtg acaaagacaa aggtaatgcc caatcgttgt
    36121 tgtaattcac gcaattcgta ctgcatgtct gttctcaatt tcaagtccag cgctgataaa
    36181 ggctcgtcca acaagaccac acggggttgg ttgatgatag cacgggcgat ggccacacgc
    36241 tgacgttgtc ctccagaaag tttgcggatg gaacgttttt cataaccttc caactgaacc
    36301 atcttgagaa cttccgctac acgctgctcg atttctttct tatcaatttt acgcaagcga
    36361 agtggaaagg caacattttc aaacacattc atatgtggga acaaggcata ggattggaag
    36421 acggtatgta cgtcgcgctt gttggttgga atatcattga tacgaacacc gtctagcatg
    36481 atatctcctg tcgtcgcatc cagtaaacct gcaataatgt ttaggatagt tgatttcccc
    36541 gaaccagatg cacctagaag ggtgtagaat ttcccttctt ccaactcaaa gttgatgtct
    36601 ttgagaacct tggtgttgct gtcttcaaaa actttagaga cgtttttgaa ttcgataatt
    36661 ggttttttca attggcataa attccttctt tttcatagat taaccgatcg gggctctgtc
    36721 aggtccccac tacctcttgc agggagtaaa accacctgca tacatcttcg ctaccgatag
    36781 gctttcaccc aagatccgga cttctctttc aagcgtaata cctgagtgtt ccttgacttt
    36841 ttcgataacc gattggatca agtcctcgta gtctttggcc gttccatctg cgacattgat
    36901 cataaatcct gcatgctttt ctgacacttc tacgccaccg atacgatagc ctttcaagcc
    36961 agcttctgaa attaactgac ctgcaaaatg cccgactgga cgcttaaaga ccgagccaca
    37021 agatgggtat tccaaaggtt gcttgagttc acgtaggtgc gtcaagcggt ccatttcctg
    37081 cttgataacc tgatgggttc ctggagctag ggcaaattta actgacaaga caactgcacc
    37141 agactcctga atagctgaat ggcggtaacc aaaagccaag tctttagcag acagggtttc
    37201 gatttctcca tccttggtca agaccttaca agactgcaag atgtgagcaa tctcgccacc
    37261 ataggcaccc gcattcataa agacagcacc gccaacgctt cctggaatac cacaagcaaa
    37321 ctcaaagcca gttaaactat gacggagggc aatgcgagtt gtttcaatca agttagcccc
    37381 agcttctgct tcaatggtat agccatcaac agaaacgtta ttgagcttgt cacacaagat
    37441 gacaaatcca cgaatcccac catcacgaac gatgatattg cttgcattgc caagaaccat
    37501 ccaagggata ttttcttggt tggcaaattt cacaaggcga gccaactcaa aacgatttcg
    37561 tggaaagacc aaataatcag cctctccacc tacttttgta taactatagc tatgcaaggg
    37621 ttcctgaaaa cggatatcaa ttccttctaa gatttcaagc attttttctc ttacagacat
    37681 gtcactcttc cttttccaaa attcattcca ttataccatt tttagagaca tttgacgacc
    37741 ataaaaatac cttgtttgga ttttgcataa gaaaaagagg ttcccccctt tttatgattt
    37801 tttacaaaag atttccttgg ttccataggc gaccagaacg agctccagtg ctagaatcac
    37861 ttcaaccaag actggatttg tcaaccagcc tacttggaaa agagatggtg ccagatcaaa
    37921 gaaggcatgc aagccatagg ctgttaggag ataaatccat ttcttctggc gaacagcttg
    37981 gtaaacccaa actgtcaaaa gtaattggaa accaagcgcc aagattcgct caaaaccaag
    38041 caaataaatc tgccagaccg aaagtgactg aatggttttt aacatatttt cagacagtaa
    38101 ttgcataacc tgtggattct gagtttgaac tgccgaaaga acaatgtaaa gattgagtaa
    38161 actagtaagg cctagaaaaa tcaactccaa gccaccatgc cccagtccat aggccaaggc
    38221 gtctgccttt tccaaacttc tctttttctc aaaccatttg aagaaaacaa gacgagccgt
    38281 ttcctcaaaa aatgctgcca tggctaggcc atagataata tagataagcg gatgatcttg
    38341 caagagggca atactaccgt ctttttgagg gtgcaaaatc aagatatgca ccagtttttc
    38401 caaaatctgc gaagagacaa agaaagcgac tgcacccaag cccaagacag ctagattaat
    38461 ctggtatttc tttttggcaa accaaatgct tcctatcaga aaagctagca acagcaacat
    38521 ggtaataatg atatgaatgg tcattttctt ctcctattct gccttttcaa tatctttttt
    38581 catctcgtca acattgaact tagcaaacaa gtattgacgg tcttggactg ggaaacgttg
    38641 gtccaactgg tcaactgctc ccacctcgat aatgccttcc ttgatgacaa aatccatgac
    38701 atgtccaccg aaggtcaaat catctgatat gaagtgcaga tggtagcctg ccacactgac
    38761 cccatggaaa atctcaggcg tccagaaacc aacaatagtc cccgcaacat tgtcacgact
    38821 atattccggt tgatgggttg cgacatcagc aaacttggta tcgggtgttg acttaggaat
    38881 catacgcaca tgcatatgta aaaattcccc ccgaatcttg atagagcgga aaagattttc
    38941 cccatcataa taagactcaa ttcgttcttt caattccttg tctgtcatct caaagcgctg
    39001 gcggaaaatg acctctgcct gatgcggtac cactgcagcg taaggaataa gggcatctgg
    39061 tgacacttcc acaatttctg gctggtctcc tgaccctttg gcctgataag ccttgccatc
    39121 caagacaatc aattccccat caatggaatc caaggttccc aaaccaaggt caccatgttc
    39181 tagcaattct cctactgtca tagtaccacc ataaaggcca gccatcaagg ctccaagagt
    39241 attgtattga aataatttca ctggttcctg cacgttctta tccattcact ttcttgtctg
    39301 ttattctata aatcttactc ctaagtatac cacatttgcc cctagatgtg aacgagagaa
    39361 acgttctaga cattgccaag aaggaaaaaa aagggtacaa tgtaacaaaa tcaagggagg
    39421 tctggaatga agaaacaaag caagtacaaa gaggtcgttt cctatctgaa aaatggtatc
    39481 gagtctggac gatttccgac gggtagtcgc ctgccttcta tccgtcaact gagccttgac
    39541 tttcactgca gcaaggacac cattcaacga gccctgctgg aattacggca cgaacaatac
    39601 ctctatgcca agcctcagag tggctactat gtattagaac aagggcaaca tcaagaccta
    39661 gaaatcgagg ttaccgacga acatgccagt gcctatgacg atttccgact ctgtgtcaat
    39721 gaaaccttga ttggccgaga aaactacctc ttcaactact atgacaatca agaaggatta
    39781 gaagacctaa gacagtccat tcacaaactc ctctttgagc aagctctcta ctgcaaggct
    39841 aaccaactag tactgacttc tggaacccaa caagccttat ttatcctctc tcaaatatcc
    39901 tttcctagac aagccaagga aatcttggtg gaacagccaa cctaccatcg gatgaatcgc
    39961 ctcttgattg cacaggggct ggactatcaa acgattgaac gaggcattga tgggattgac
    40021 ttggaggagc tggaaggcca cttcaaaaca ggaaaaatta aatttttcta caccattccc
    40081 cgatttcact atcctctggg acattcctat tctgagcaag acaaacgatc tattcttaac
    40141 ttagctgcca agtatgatgt ctatatcgta gaggacgatt atctgggtga tttggactcc
    40201 aagaagggcc aaaccttcca ctatcttgat acagaggagc atgtcattta tatcaagtcc
    40261 ttctcgacca gcctttttcc tgcccttcgt attacagcac tcattcttcc aaatgctatc
    40321 aaagaagcat ttgtggccta caaaaatatc ctagactacg acagcaacct cattatgcaa
    40381 aaggccctgt cactctatat tgacagtcaa ttgtttgaaa aaaatcgttt ggctcgcttg
    40441 accaatcatg aatcttacca aaaacaaatc gaggaaagga taactaaaac accttgtccc
    40501 cttcctcatt atcccctaca cgatggttta ttgctagacc tgagacagta tcctaaaatc
    40561 gccagtctca aacacagtca actgggcttg gacttctttg aagaggccta tttgagcacc
    40621 tgtccttatc aatttgccaa ggtgtcccta gacaatctgg aaaatgtttt aaactattta
    40681 aaagcagaat tggaatgaca tccaactctg ctttttctta ttcttcaact tcagttagac
    40741 ttgctgcttt ttcaagatag ctttggtaag ttccttcatc cttaagtttt tggatgacct
    40801 tgtctaccac ttctttcaaa tcatcactat tttttctaag agcaacggca ttggcgtcgc
    40861 cgtccttcat cttcaagctg acagttgcga cagctaagcc agcgttttta gcagcataac
    40921 taagtgcaac aggctcatcc atatgaacag catctatttt tccagcctgc aattcattga
    40981 ctgcttcacc catattagtt agggaagtta attgagcttt tggcaattgt tccttgacca
    41041 ttgattctgg aacagtccct ttttgggctg caatattagc actttctagg ctagttaaat
    41101 ccttgtattt ttccacatca gccttacgaa ccaagaaact aatcttgttt tcatagtatg
    41161 ggattgaaaa atcaaagact tcttttctct cgtcagtagc actaattcct gcaactgcta
    41221 ggtcagcctt accagtttga agactggtca aaacattgtc aaaactcatg cttaagattt
    41281 ccaacttaac cccaagttca tcagcgatag cctgagccat gtcgatgtct gcaccgacta
    41341 cctggttctt tccatcaacc aatgattgaa attcaaaggg tgcatagtca ggactggttg
    41401 ccacaactaa tttccccttt tgcttaatgg cctcaacagc tgactgagaa ccattagaac
    41461 ctgactggca agctactaag aagaaacttg caagaaaatt acataaaaca aatatccatt
    41521 tttttgattt cataaataaa caacctttct gttaattttg tataattata acgatatcca
    41581 agttacttgt caagtgtttt ttaaattttt atctcaaaaa tattttttcg ttcaaaaaaa
    41641 ggagccatca gttgatttca agctcctttt tatacagaat taaactattt tatagttcga
    41701 caatcttacc tgtttcaaag tagacaaccc attcacagat atttttagca tagtcaccga
    41761 tacgctccaa gtaggaaata acttggaaat aatcacgacc cgtaacaatg gcttctggat
    41821 ttttcttaat ctcttcagtc gcaaggtcac ggatagtttc aaaatagtgg ttaatttgct
    41881 catccatgga ggccacccgg tatgcgtcgt caacagaacc attaagataa agatcaagtg
    41941 ctgcttccac aacgctttta acttcacgtc ccattttttt aatttcttcc tctacagctg
    42001 ggatgcgttc ttcccccttc atacggatgg ttgcctgggc aatggctaca gcgtgatccc
    42061 ccatacgctc cacatctgat acagccttaa ggacagtcaa gactgtacgc aaatcttgag
    42121 agactggttg ttggagtgcg atcatttcaa atgatttctt ttccagtttc acttcgtatt
    42181 catttacttc tgcatcatct tcgatgacct cttttgccag gtcacggtca tgcgtgacaa
    42241 aagcacgtac cgtacgattg atttgtgaga gcacttcttg tcccatagcg tagaactggt
    42301 tatgtaattt ctctaaatct tcttcaaatt gagatcgtaa catctttcat ctccttatcc
    42361 aaattttcct gtaatatagt cttccgtttc cttgtgttgg ggatcaagga acatctgctt
    42421 ggtatcatta aattcaatca aatctccatc taggaaaaat cctgtcttat cagagatacg
    42481 tgaagcttgc tgcatggaac gggttaccag aagcatggtg tacttgtctt ttagaccata
    42541 caaggtttcc tcaattttac cagctgaaat cggatccaaa gccgaagttg gctcatccaa
    42601 gaggatgatt ttaggactag ttgccaagac acgggccacg cagacacgct gctgttgacc
    42661 acctgacaat ccaatagctg aatcatatag acgatccttg acctcatccc agatagaggc
    42721 accttgcaag gctttttcta cggcttcatc cagaacctgc ttatccttaa ttccattgat
    42781 acgaagcccg tagacaacat tctcatagat agtcataggg aaaggattag gttgttggaa
    42841 aaccattccg atttccttac gtaattcaac cgtatctgta cgcggactgt agatgttgtg
    42901 accattgtac accacggatc cagttgtggt cacctctgga ttgagatctc ccatgcggtt
    42961 gagagacttg aggagggttg acttccctga tccagatgga ccaatcaagg ctgtaatttc
    43021 cttaggttgg aaagataggg aaacactatt caaagccttc tttttattat aatatacgga
    43081 caggtctgat acctgtaaaa tcgcatctgt catacggttt cctttctaac caaagtgacc
    43141 agatacatag tcattggtgg actgtagctt ggcattttgg aaaatagttg cagtcttgtc
    43201 atactcaatc aaatcaccca agtaaaagaa gcctgtatag tcacttgcac gagcagcctg
    43261 ctgcatatta tgcgttacaa tgatgatggt aaagtttttc ttgagctcaa acatggtctc
    43321 ttctagttgc atggtcgcaa tcggatccaa ggctgaggct ggctcatcca ttaagaggat
    43381 atctggctta acagagatgg cacgagcgat acagagacgt tgttgctgac cacctgataa
    43441 ggtcaaggct gacttgtgga gatcgtcttt aacctgatcc cagagggcag cctgacgaag
    43501 ggaggtttct acgatttcat ctaggacttg cttatcctta actccagcac gttcatgcgc
    43561 aaaggtaata ttacggtaaa ttgacttagc aaatggattg gggcgttgaa aaaccattcc
    43621 aatgtgttta cgcatttcat aaacgttgat ttctggacgg ttgacatcaa ttccacgata
    43681 gagaatctgc ccagttactt tagcaatatc aatagtatca ttcatgcgat tgagactgcg
    43741 taagtaggta gatttccccg atcccgacgg accaatcaaa gctgtaattt tatttctttc
    43801 aaattgcata tcaatcccct taatggattc atttttacca tagtaaacat ggacatcctt
    43861 agtagaaagg gctacttttt cttcaggaaa ggtaaggata tgcttctcat cccagttata
    43921 tgttgacatg gcttctcctt taggcagcgg ttaatttctt gtgtagatag cttccgaact
    43981 tacgagctcc aaagttaaaa atcaggataa agatcaggag cacagcggca gaacctgctg
    44041 atacaatggt tccatctgga atagtgcctt cactattgac tttccagata tggacagcca
    44101 aggtttctgc ttgacggaag atagagatgg ggctagtcac actgaggata ttccagttag
    44161 accagtcaag agctggcgcc gattgccctg ctgtatagat cagagctgca gcttcgccaa
    44221 agatacgacc agatgccaag acgacacccg ttacaatacc tggaagcgct tctggaataa
    44281 caacatgaac cactgtctcc cagcgagaaa tcccaagagc cagaccagcc tcacgttggg
    44341 tatggtgaac gtgtttcaaa ctatcctcta cattacgcgt catctgaggc aagttaaaga
    44401 ctgtcaaggc caaggcacct gaaatgattg aaaatccata ctcaaactgg actacaaaga
    44461 tcaagtaacc aaagagaccc accaccactg atggtaaaga ggacaagatt tcaatacaag
    44521 tccgcacaaa gttggtaaca ggaccttttt tagcatattc agccaagtaa atcccagctc
    44581 ccatagaaag aggtacagaa ataatcaagg taatgaccaa taggaaaaag gaattgtaaa
    44641 gctgaatgcc aatcccacca cctgcttgaa aagcagaaga ccttccagtc aagaaagacc
    44701 aagagatatg gggcaagccc cgaaccaaga tatagagaat caaggaagcc aagattgtca
    44761 caatgatgct agcaatcgta tagaggacag ctgttgcaag tttatctaat ttcttagcgc
    44821 gcataatttt tctttcctct ttctttcgta atcaatttaa tcacactgtt aaaaactaaa
    44881 ctcatcaaga gcagtaccaa ggccagtgac cagagaacat tattatttac agttcccatg
    44941 acagtgttcc caattcccat agttaatata gaagttaaag ttgcagctgg tgtggtcaag
    45001 gaagttggga taacagctga gtttccgaca accatctgga tagctaaagc ctcaccaaag
    45061 gcacgcgcca tcccaaagac cactgcagtg aaaataccag aacgggccgc cttcaagatc
    45121 acacgccaga tagtctgcca gcgagtggct cccatagcga aacttgcctc acgataataa
    45181 cgaggaaccg cacgcaagct atccgttgtc ataaaggtta cggtcggcaa aatcatgaca
    45241 aagaggacgg aaatccctga caaaatccca aaaccagtcc caccaaagac actgcgaaca
    45301 aagggaacga cgacttgcaa gccaataaat ccgtacacta ctgaaggaat cccaaccagg
    45361 agttcaatag ctggttgcaa aatcttcgcc ccttttggtg atacttcggt cataaaaact
    45421 gctgcaccaa tagcaaaggg tgttgcgata agggctgaga gaatggtaac gataaaggaa
    45481 cccaaaatca taggaagggc accaaattct ttactagaag gattccaagt tcctcccaaa
    45541 agaaagtcaa agatattcac accattgaca aagaaggtcg acaagccttt ttgcgctacg
    45601 aaaaccaaaa tcatggccac aaggatgact atcaaagaaa gacaggcaaa ggtcaaacct
    45661 tttcctaatt tctccagacg agaattcttt gatggaagca acattttctt agctaattct
    45721 tcttgattca ttattgtctc ccttccaaca ctgtcacagt tccggcagca tctttttcaa
    45781 ccttcatttc cttaatcgga atatacttca atcctttgac aatcccttct tgggtctcat
    45841 ccgagagaac aaaattgaga aattctgcag ctaactcatt gggctgcccc aatgtataca
    45901 tatgctcata agaccacaag ggccaattat tgctacttat attttctgga cttaagtcat
    45961 agccattcaa cttcatgctt ttgaccgaat catctatata ggtaagagat aaataagaga
    46021 tagctcctgg actttttgat acgattgatt ttaccgctcc atttgaatcc tgctcctgac
    46081 tttgcatggc agactgacct tccataatga cagtatcaaa ggtagcacga gagccagagc
    46141 cggctgcccg attgataaca gagatgggta agtccttacc accaacctct ttccaattgg
    46201 ttacctcacc tatgaagatt tgacgaagtt gctctgtcgt taggttatca acatcaacct
    46261 ccttattgac aatcagagcc aagccagcta ccgcgacctt gtggtcaaca agagcagaag
    46321 catcaattcc gtctttttcc tcagcaaata catctgagtt tcctatatca actgccccag
    46381 actgaacctg ggacaagcct gtaccagaac ctcccccttg gacattgacc gtttttccaa
    46441 catggatcgt gccaaattca tctgccgcta cttcaaccaa gggttgcaag gcagttgagc
    46501 caacagccgt tatggattct ccacgatcaa tccagctagc acagcctact aaacaagccg
    46561 tcagccaaaa agcgataaga gacagagcaa gcttttttct ttttttcact gtttttctcc
    46621 tcgaaaataa ttatgaatac tgtgaatttt ttaagtagtt ctttatgagt tgacgcatga
    46681 attcttacca aatttctgcg caattgatta tttatataat ataggctata ttactctttc
    46741 ctaacctcct tttttcatat gtggataaaa tctcttgtct atcccttccc ccattgtcac
    46801 ccattatagt catttcgtgt ctctttttcc cctttttaat gcaagggaaa ttactctcct
    46861 tagatgataa tccaaaagct agaaaggtat ctcaaatctc tctactctcc cagactagtt
    46921 tacaactaaa aggaaaagat tctattttat gagaaatcta gtttacaagc ggtaagaacg
    46981 ctaataacta aacttcttgt actctttgaa aatctcttca aagcagtgtt ttgagctatc
    47041 tatggctagc ttcctagttt gctctttgat tttcatcgag tagtaaaact acatgtgatg
    47101 gcaatcaaga tatcaagaat catcctacta aaaaaatcca tactttcact ataacataga
    47161 ataagatatt tgactagcat tttcatttga atctgaggcc ttttggaaaa taatttttca
    47221 aaacatttcc agtaaccttt gcaaagccca agccattgcc tttaaccaaa acttggtacc
    47281 aaccatttgg cagactttct gccagctgaa cggtttctcc agccgcatac ttgacaaact
    47341 cttcttggcc aatttcaacc gactgttcga cctgactcgg tttcaaggct aaaccaagag
    47401 cgaaactggg ctcaaagcgt ttcttcttaa aagtacccag atgcagtcca ttgcgagcaa
    47461 tcttgagctt ccctaaatct ggcaaaagtt ctggcaagag ataaagctgg tctccaaaaa
    47521 tctgcaagat acccggtaga ttgaccttca aatggttttg ggcaaattcc tgccacaagg
    47581 caacttgttc acggctgagg ttactcttac ttgccttaaa tttaggagct ggattgttac
    47641 ccttaaactg tagatgggca acaaactgac cctctccctt aaactgatga gggtacatcc
    47701 gagccgtttc tggcaggtca ataccagcta ccattccatt gatatgctct actggcaaca
    47761 agtcaaaatc atactcttcc agcaaccaat tgacaatctc ttcgttttcc tcgggtgccc
    47821 aggtacaggt cgaataaacc agacgaccac cttcagctaa catggtcact gcatcctcca
    47881 gaatttctct ttgcaagcta gcacattgac tcggataatc taagctccaa tagtccatag
    47941 catcaggttg cttacgaaac attccttcac cagagcaagg ggcatcaaga acgattaagt
    48001 caaaatagcc tttaaagacc ttgaccaagc ggtcggcaga ttcattggtc accacgacat
    48061 ttgtcgctcc aaaacgctcc atgttttcaa ccaaaatctt agcccgtttg cttgaaattt
    48121 cattggaaac aagtagcccc tcccctgcta gataggctgc cagttgagtt gatttgcccc
    48181 ccggtgcagc agccaagtcc aagaccttca taccaggact gggttgggct acttgagcca
    48241 ccatttgagc agcaggttct tgcgaataaa ctaaacctgt agcatgctca ggcgatttcc
    48301 ctgaaacctt cccatagtgg ccccaagggg tttgagtaat ggcatcagaa aaggaaagtt
    48361 gctcttcttt taagggattg acccgaaagg ccgaaaccgc ttcctcctca aaagaggcaa
    48421 gaaaatctct tgcctcatct cctagtattt ctttatattt ttcaacaaat ccttctggaa
    48481 atttcattta agttcttttc ctttcgtaaa tataggactg aatttcctcc tgcatctcaa
    48541 gaggcaccat catgaccggc tgtctggttt gaaaatcagg agcttcacca aaaagggtca
    48601 caacccgata gcccagactt tcccctaaaa tactagctgc ggcataatcc catggttgca
    48661 gataggtgag ataggtcaac aaacgccctg acaaaatctt ggcaaaacta atggccgcac
    48721 ttccatagac acgaacacca agaaccgctc ggctcaaatc agccagcccc cattcattgg
    48781 tttccagcat accactattc cctgcaatga gaaaatctcc aagtggttta gttttaaaag
    48841 aagctagggg cctatcattt agacaaactg gaaattcccc accaccgtgg taacaatccc
    48901 ctttgaccac atcataaatc agaccaaact gtccctgacc attttcaaaa taagccatca
    48961 taacagcaaa atcttcctgc tgggctacaa aattattggt accatcaatg ggatcaatga
    49021 cccaaacctt gccctcttga accgaggctc gcagacaacc ttcttcagca caaatcttat
    49081 cctcaggata acgggacaaa atctcaccaa ccaagagttc ctgaacttct ttgtccagtc
    49141 tggtcaccaa atctgttgga gaggacttgg tttcaacacg caagtcttcc tgcatatggt
    49201 caagaatgta ctgacctgct ttcttaacaa gctctttagc aaattcaaat ttacttttca
    49261 agagaaatct ttcctttccc tttttctttg gcagcctgaa ctgctcggta aagtgaatag
    49321 ccactaaccg tttcaaattc tcgtcccaaa cgtttctctt cgctcttgct tggcacgacc
    49381 gctttgaatc ccttatagga gtccagtaac tttttagcct ctactttgcc ttcataggca
    49441 gcttcaacat cattaaaaaa agaaagcact gaagcaagtt cttcagtgct ccacgacaaa
    49501 tctagtgggt aactatactg tttgttcatt aactaatacc agctctcatt cttgcttctt
    49561 ttagttcttg cttacgataa ctacgaggga gaaaagcacg aatctcatct tcattaaaac
    49621 cgatttgcat acgcttggca tcaataataa ttggacgacg caaaagacta ggatactgct
    49681 caatcaaatg aagcaattcc gataccgaaa tactctctac atcaatattc aatttttgaa
    49741 aaatttttga acgagttgaa atgatgtcat cagtaccatt ttcggtcaag gaaaggatgt
    49801 gttgcaattc ttttcttgtt aaaggactgg tcataatatt gtgttccaca aagggaacct
    49861 tatgtttttc taaccaggtc ttagccttac gacatgatgt acagctcggt gatagaaata
    49921 gtgtaatcat gcttttctct tcttatctat actttgctac ttctattata caaaaaaata
    49981 aggcgcttga ctagggattt ttagaaaaaa agcctatttt ttcaggaaaa ataggctgtt
    50041 tgcgaacgat tgacacaatt ggatttggtt aattcactct taacgatggt tttaaacgat
    50101 atatatattt atatatgtaa attaaaaaca tctttccttt cacttcctac gacttttcag
    50161 acacagatag ccaaagaagc tttcatagag ggcaaaaaag aggaggaagg catgaagaaa
    50221 gaaggtctct ggcaaaatca taataacagg atccttggct ggatcaaaaa gccaggtatc
    50281 atctcccaca aagagaattt gatggaaaag agtaaagaat tggtcaaaac caatcaaaac
    50341 tcccccaagt ccaatcatca caggtaagac tactagagcc aggagacttt ttcgataaag
    50401 agacaaaaag tcctttttca caatcctatt gacaaagaca tagaaacttg gcagtgtcac
    50461 tagagctact agctgaacca aatgaaagag attcttgacc actgcgaaat ggtgcaaacc
    50521 agctgctgac gaacgaaaat caggcatctg taagacctga ctaaaaggat tggtcagata
    50581 attcatcaag atatgaaaat tgtattgaat ggtttctggt tttagataga ctcgattcgt
    50641 taagtttagc cactgaatct ccataggata gaaaatccaa gccagataaa tggtcagaag
    50701 gattgagagg gagaggagaa agagcataga gccccaaaag gtcaatttag ttttcatcaa
    50761 aatcccactc cgcaaggcta gaaaccacat gtgtcggtgc gattggcagg ccagctactt
    50821 cttctgcctt ggtaaaacct gtcgtcacca agagcgttgg aatgccattg tcaatcccag
    50881 cccggatatc agtcaaataa ttgtccccaa ccatgattaa ctcttcacgt tccaaaccta
    50941 agtgctcaac tgccttgtcc ataatgatgg cattgggttt tccgatataa accggcttca
    51001 ctcgtgtcgc tacttcaagc agcgtaatca gtgagccagc acctggcaaa agaccacgtt
    51061 ccgtcgggat gttgaggtca ggattggttc cgataaaatg ggcacccttt tgaatagcaa
    51121 gagttgctgt ggcaaatttt tcatagtcga cttgccaatc cagaccaact accacgtagg
    51181 caggtttttc cttgtcttcc acataaccag ccgccttgat ggcttccttg agtcctgctt
    51241 ctccgacaac atagacggtc ttttcaagcc ccaaatcatt catatagtcg atggttgcca
    51301 aagtcgctgt gtagacagtc gataggggcg tatcgatatt aaaattctga gccaacatct
    51361 ccttaacact ctctggagtg cgggttgtat tgttggttac aaagagatag ggaatgtccc
    51421 gcttttgcaa ttcatgaaca aaagtctctc cagcagggat ttggtctttc cccttataaa
    51481 tggttccgtc taaatcaatt aaatagcctt tatatttcat ctatttctcc ctaagccttt
    51541 tttatttctt gccaagtaat gattgcttgg gcattgataa ccccatcact tgtaatttca
    51601 tgcttgcttt ccagtccagt ccgttcaaca gccgatgtaa tcaccccacc tggtcgaact
    51661 tccttgacat acttgaggtt gattttcttg ggaatatagt gggtcaaaaa atccgctccc
    51721 atgacctcaa aaatccagtc taagtattta ctgttattga catgaccatt catatccaag
    51781 tcgtaaaaac gaacatggta atccttgctg atcgactctt ccaaggactc atacttcggt
    51841 ccacggataa gttttttatc aaaatcagac tggtaaggag ccacaatctc aggttcaaca
    51901 gcatggactt ttcgactgtc gcggtccatg agaacaaagg tcgccatcat atggatgagc
    51961 tcctgccccg cttcattata aatagtaaag cgacggtagc aaaaaagtcg attgtagctc
    52021 aaggcttccg tttcgatggt aatttcttcc gcaaaacgag gcaaacgaac cacctcaata
    52081 tcatattcta cgataatcca gaccagatta tagtcttcca aaatggcctt atcactaact
    52141 cccagttcaa tcgactgcat ccctgaaact tgcagtgaca gcaaaattac atctggaagt
    52201 ttgatatgac cgttcatatc agccatatca aaaggaattt tcattttcat ttgataagtt
    52261 aagcccatga tcctactcca aaataaatcg ttccgctaca gtatctccca aaaagagacc
    52321 tctctttgtc atgcgaacgt ggtcaccctc aatctgcatg aggccttgtt gaaccaaatc
    52381 tctgacaatt tctccataaa gtccagcaaa agactgtcca aatttttcct caaatcgcgc
    52441 catggaaacc ccggatttct tgcggagtcc caagaacatt tcttcttcca tttgctcctt
    52501 ttgactcagg tgatcttctg taatacaagc attgccttcc tcaaccgcac tgagataatg
    52561 acgaatggga ccatgatttt tatagcgtac tccattgaca taaccagatg cccctgcacc
    52621 aataccatag tattcagcat tgtcccagta catgagattg tgacgacttt caaagccggg
    52681 tttggagaaa ttagaaatct cataatgctc aaaacccgct cgctccagct ctgcaatgat
    52741 gtactcaaac atctccgctt ctagttcctc cttaggcaga ggcaatttcc cacgtcgcat
    52801 ccgattcata aagaccgtat ggttttctaa aatcaaacta tacaaactca tgtggggaat
    52861 atccagtcca atggctttag ccacattttc ctttacttgc tccatggtct gaccaggcag
    52921 agcataaatc aaatcaatgg agatgttgtc aaaaccagcc agtttcaggc gatcgatatt
    52981 ttcataaata tccttctcca aatgactgcg cccaatcttt ttcaacatct tatcatcaaa
    53041 ggtctggaca cctagcgaaa cacgattgac agccgaattt ttcaaaacag ctatcttatc
    53101 cgcatccaaa tcgcctggat tggcttcaat ggtcaactct tctaagacag acaagtccaa
    53161 gtttttagtc aagccattca gtaaaacctc cagttgcgga gccgacaggg ctgtcggtgt
    53221 tccaccaccg atataaaggg ttgacaactt ttcaatatca taagaacgaa actcttccag
    53281 cagatgctct aaatagctgt cgactggctg atttttgatg aagacctttg aaaaatcaca
    53341 ataataacaa atctgggtac aaaatgggat gtgcacatag gctgacgttg gttttttctg
    53401 catagtaatt attataccac aaagactaga ttccagataa aaatcaccat ccccagatac
    53461 atagtccgtc cggagatggt gatggtttat tcttctgtta tatcaatcac aatctcttct
    53521 gagtcatcaa gagcttcggc tttttcttgc cattgctcct tgagattatt taattgattt
    53581 tttgatgctt ctgtcgcttg aaaagcatag gatttagctt gagcaagtat actgtccaca
    53641 gtgatttcac ctgactcaac ctgttctttt gttttcagaa caaaatctgt agcctgctcc
    53701 ttaacttctg tcagtttttc acagacttgc tccttggcat actccggatc ttctctcaaa
    53761 tcatctagaa aatcttgagc ctgactgcaa acttgtttgc ccttatcact tgttaaaaac
    53821 aaggcaagag ctgcacctga aacggttcct aaaaggattg aggataattt acccataagg
    53881 attctccttt tttatttttt gaaaaattta cttgcaagac gaagagctga cagacttgca
    53941 ccagtcttga gtgtttttga accagctgat gaagctttct tgctcaagac acgcgcatgg
    54001 tcattgaggt ctgaaacaga tagagataaa tctgcaacag cactgaagag tggatcaatc
    54061 gtagccacct tgacattgat atcatctgcc aagacattga ccttagccaa caactcattg
    54121 gtgtgatgca aggtcacatc cacatctgaa gtcaaggttt taatcgtctt ttctgtttca
    54181 tcgatgacac gaccaagctt ttgtacagta atgatcagat agaccaaaaa gacaatcaaa
    54241 gctagggcaa caagaatata tgcaacttct aacatttagt tttcctcctc tgtaatatag
    54301 taaggggcct tctttcgatt ttgataaata acgatcatta taccgagacc gataaggaca
    54361 actgacagcc attgggacac tcgaaggccg aagaacatga gactatctgt tcgcatacct
    54421 tcgatgacca tacgaccgaa accataccaa atcaagtaaa aggccgtgat atgacctcgt
    54481 ctgagactct tccatttccg tctaaaaatc agaatcaagg caaagccaag cagattccat
    54541 agagactcat aaaggaaagt cggttgacgg tagctcccct caatatacat ctggtcacgg
    54601 ataaagccag gtagataatc cagattatcc actgttgcac cataagcttc ttggttaaag
    54661 aaattacccc aacgccccaa actttgagca atcataacgc taggcgccgc aatatctaga
    54721 aaatcccaag tattgatgag tttacggtca gcaaagatat agagcacaag agccccagtt
    54781 atcaaaccac cgtaaatggc caaaccacca ttccaaatgg caaaaatctc tcctaaattc
    54841 tgactatagt aatcaaatcg gaaaataaca tagtagagac gagctcctaa aatagccaag
    54901 ggaaaggcta ctaagataaa atctaaaata tcgtctggta tgatcttctt tctaggtgct
    54961 tctttcatgg tcaaataaac cgcaagaatc aagcctgtca caatacataa ggcataccaa
    55021 cgaatggcta ggggtcctag ttgaatagca attggatcaa gcattttgca cctcatttcg
    55081 agcgatgaga cttgtcagtc gttcgtcgaa caaacgggtc gcatcaaagc ccatttcctt
    55141 ggcacgataa ttcatggcag ctgcctcaat cacaacagag atattacgac ctgttttaac
    55201 tggaatacga atacgaggaa tggctacgcc agaaacttca agttcctctg cattgtttcc
    55261 aagacgatca aaggtcttat gcgtatcgta attttccaaa tagacagcaa gctgaacctg
    55321 tgaagaatcc ttgacagcac tcgcaccgta gagactcata acatcgataa taccaacccc
    55381 acgaatttca atcaagtgtt tcaaaatttc agctggttca ccccagagag taatctcatc
    55441 cttggcaaag atatcgacac ggtcatctgc taccaaacgg tgaccacgtt tgacaagctc
    55501 aagacctgtc tcgctcttac caattccact atctccctga atcaagacgc ccatcccata
    55561 aatatccatc aagacaccgt gcacactggt acgttctgcc aaacgagaat ccagatagct
    55621 agataactct ccagataaac gactggtagc tgcacggctg gttaaaatag caatcttaca
    55681 ttctctagca gcctttaaca tctcctctgg aaccaccaaa ccacgggcaa caatgactgc
    55741 tggtgtctca gatagaaaca tttttttcaa aacttcataa cggctgttgg aaggcatgct
    55801 gatcagataa gaccactcct tcatccccaa aagttgaatc cgctctggtg tgtagtagtc
    55861 aaaatagcct gtcatttcaa gaccaggtcg cgtaatatcc gctgtattga tttccttttc
    55921 aagcaactct ggttcaccat agacaatatc tagtctaagc ttttcaatca cttcttttac
    55981 taaaaccgac atcatttcct ccttcaaatg agttcttctc tctattatat caaattgtag
    56041 gaggaagttt cagatttttg caggctttgg agtatttatt taaaagaaaa aggactagat
    56101 ttctctagcc tttcattcca attagaattt tttacgttta cgagctactt ctgatttacg
    56161 tttacgtttt acagaaggtt tttcatagaa ttcacgtttg cgtgtttctt gaagagtacc
    56221 agctttagta accgcacgtt tgaaacggcg aagtgcgtcg tcaagagatt cattcttacg
    56281 tactactgtt ttagacattt ttttcactcc cttcaagttc gatatctatt tcattctaca
    56341 cgtaaaagga ataattgtca agcaaaaatg ctgtttaatt ttgatttttt cttccattta
    56401 tacaagagtt gaaagcgttc aatagaacat tgctttttta tttttcgagt aagctaagcg
    56461 cttcagcatc tgcgatgatg gttacatcag ggtgattttg gaggctactt gcaggtaggt
    56521 tctcagtcac tgggccagat actgttccgg caatggcttc tgctttcgac tcaccgtaag
    56581 caaaaagaat aatagacttg gcatccaaaa tgtttttaat ccccattgaa atagcttggg
    56641 ttgggacgtc ttcaatcttg gcaaagaagc gtgcattggc ttcgatagta gactggtcaa
    56701 gttctactag atgcgtttga ctgtcaaatg gagtaccagg ctcattaaag ccgatatgcc
    56761 cattgcgacc gattcccaag atttggaggt caactggatg gtcagccaaa atttggttgt
    56821 agcgttcaac ttcagcttca gcattgtcct taacaccacg aggcaagaaa ctttctttaa
    56881 atggtttttg gttgaacaag ttttcttgca tgaagtaacg ataagactgt ggattgtccc
    56941 catcaaggcc cacatactca tcaaggttga cactggttag atttgaaaaa tcaaggtcac
    57001 tctcaacaat ttccttgtaa aattcaagtg ggctacttcc tgtcgcaagt cctagagttt
    57061 gagctccatt agccaatttt tccttcaaaa tctcaaaagc aacttttcca ccttgaactt
    57121 ggttttcaac tttaataact ttcatctttt catcctccat ttctgtctat atttattata
    57181 tggtatagac caatttttgt caagtgaaaa cgatttaatt tctaaaaaaa gagcgtccaa
    57241 acttgaaacg tgttataatg gataggatta gaacttagaa agaatgaaca gatatatgaa
    57301 tacagctgat tttgatttcc acttgccaga ggaattgatt gcccaaacgc cccttgaaaa
    57361 acgtgatgcc tccaaactcc tcatcgtcaa ccgtgagaca ggagaaatgc aagataaaca
    57421 cttccactct attattaata tgctggaacc tggtgatgcc cttgtcatga acgacacccg
    57481 agttctccct gcccgcctct atggtcaaaa agtggagaca ggaggtcatg tggaacttct
    57541 cctccttaag aacactagtg gagacgagtg ggaagttctg gctaaacctg ccaaacgcct
    57601 caaggtcggt actcgtatca gctttggtga tggccgcctc agcgctgtcg ttacagaaga
    57661 attgacccac gggggacgca ttgtccgctt tgaataccaa ggaattttcc tagaagtctt
    57721 ggaaagtctg ggagaaatgc ctctgccacc ttatatccac gaaaaattag atgaccgtga
    57781 acgttatcaa accgtctacg ccaaggaaag cggctctgct gcagcaccga ctgctggtct
    57841 tcacttcacc aaagaactgc tggcagaaat ccaagctaag ggtgttcatc tagtctatct
    57901 gactctccat gtcggactcg gaacctttag acctgtttct gtggataatc tggacgaaca
    57961 cgaaatgcat tcagagttct atcaactttc tgaggaagct gctgccaccc ttcgctctgt
    58021 caaagaaaat ggtggtcgcg tcatcgctgt cggaaccact tctatccgca ccttggaaac
    58081 tattggttcc aagtttgatg ggcaaatcca agcagattct ggttggacca atatctttat
    58141 caaacctggg tatgagtgga aggtcgtgga tgccttctca accaacttcc atctgccaaa
    58201 atcaactctg gtcatgttgg tttctgcctt tgcaggccgt gaattagtct tagatgccta
    58261 ccaccatgcc atccaagaac actaccgctt cttcagtttt ggtgactcca tgtttattta
    58321 ttgagaaaga atttctctaa atcttctaat accaataaat cgctaagata ttatttcaaa
    58381 gaacatctac aattgaaact ctagctagct gtagaagacg cctagtacat tgaaattaaa
    58441 atgcttcccc ctagcttcga aaatattgcc atagattgcg ttgactctcc aaattgattc
    58501 atctatattt tatttcagct tcctatattt tcttcgctgt ttgtaaatca aaatgcaaga
    58561 cacatgagta gcaccatatt tgttactctt atctgtcctc tcaagagact attatgagtt
    58621 atttcagaat cattcactac tttgaccctg actctcctta gtctcaaaat caaagactta
    58681 tactcttcaa aaatctcttc aaactgcgtc aacgtcacct tggattatat atgtgactga
    58741 cttcgtcagt tctatctaca acctcaaagc agtactttga gcaacctgcg actagttttc
    58801 tagtttgctc tttgattttc attgagtatt aaacaaaaag tgaacaaatc tgaattctaa
    58861 tgtacagaag actaggcttg ttcacttttt tatagtcgct ataagatgac cttatctata
    58921 gctttttata tataattata tataattata tattcagaca tactattatc aattttgtcg
    58981 cagggaggaa tctgttaacg cacccattca ccattatcat tgactctata gccatctata
    59041 cttgtattga ccgctaactc acccgatgca tttacataat accatttacc accaacttgg
    59101 aaccattgat tgactttcat agaaccgttg ctgttgaggt agtaccatga accattaact
    59161 tgtacccaac ctgttgccat ggaaccatca gtattataaa aataccacat accattttct
    59221 tgtttccagt ctgttgttgg agcaactgct ttagctggtt ctactgctac atctgttcct
    59281 tggttagatg taacagatac aggatacgaa ggaatagatg attgctcagg aacaacaact
    59341 ttttcaggtt ctctcgtccc tctccttata cgtcttttta ccatctcttt agtaatttga
    59401 cgagaagtag tttcttcaat tgttccatca cgttcatcta cagtatagat tgtagtaaga
    59461 gtaatttacc aatttctcct acttcttcta cttcttgact tttatcaaga gttgggtcat
    59521 cgagatattc tgtttcgatt ggaatttctt ggacaagaac ttggggcttg gttccttttt
    59581 taacaactct tgtttgagag tcttcttttt gacttaaagt actctcagtt acttgtccac
    59641 tctttccatc tacatcataa gttatcgttg taactgtttt cccattcttt cctagagtaa
    59701 tctcttgctc ctgtcctgca gaaaggtcat tgtctgcttc atatttagta gcacatggaa
    59761 caagaacttc ttcaaccttg cttttagctg gaactttgat aactgtatcc gtggcttgtt
    59821 ttctatcaac agtaacctgt tcggtaacat gaccagtctc tggattaaca tcgtaggtcc
    59881 ttgtcgtagt tacatagcca tcctctccat caattgtaac aggattttca ctacggtctt
    59941 ttgtttcatc tttttcataa cgaattcgcg tacttgaaat tttcttggtt actaccttag
    60001 gtttagtcgc tacttttaca ataatacccc cattgtcagc gtcatcatac tctattccct
    60061 cttctttatc tctagtatca tctctgacat attgaatccc atcagcagca tgaacaaaac
    60121 ttgtattcag attcctccta aaaataaagt tagcccgatt accgcagaac caaaaatctt
    60181 tccgagttta cgtaatgcat agcgcttatt agtattagat tttgccatta catcctactt
    60241 ctagtatagc atcttttcta tcaaacgtta aacaatatac tttatatata aaatagactt
    60301 agaatgatat attgattatt gaactaacac tttaactata tcgtaatcaa tctcatatat
    60361 aaaggattgc agacatctta tctaaataca tgctaatata tttagataca aacattccaa
    60421 cttgataatt ttcactcatc tttcatcatt ccttatacaa ctatgcagta taaatagaat
    60481 agttttctca tcagaatgag actattttaa tattagataa tttgatagaa aaaacatgaa
    60541 taaaattggt tgaatttaat tccctaactc gatagaggat tctattttaa atttaacaac
    60601 agctaggatt acactataaa aagtacatta ccacctgttc ggctggagaa tctagtcgat
    60661 tagctccctc ttagttacaa gcttaaagcg attttttaac taatactaaa ccagattaaa
    60721 gaaggaacta gaacatctgt cttgtactaa tcaatatgct attttaaagt ttcaaataaa
    60781 cttggaatgc taattattat atttacaata aatagttgac ttcgatagaa acagaaaata
    60841 gaattcaaca ttcttcccta cttataccat aataaataag atccgcaaga tagccttttc
    60901 tctctacacc attaacaatg gtttgtagat aggacattcc agccttttcc atgacacgac
    60961 ctgaagctgg gttgagactg gcataacgtg ctctgacctt ttgaaaacct gcttgagtaa
    61021 aacaaaagtc caagatagct ttcaaagtct ctgtcatcat accatttccc cagtaagcct
    61081 tgcctaacac atagccaatt tcacagctta aatcagcctc gtctatctta acgatgctga
    61141 tatctcctat cacttgctct gggttttctt ttagacaaat ggcccattta taatagttga
    61201 gattagtata ggaagcaacc caattgcgaa tcgagtttcg agtgatttcg atatcaggat
    61261 ggggatccca ggtaacatag gtcagattct cagcagatga agcccaattt tgaaacatgg
    61321 cttctgcatc actctccaca aatcttctta aaatcaaacg ttctgtctgt aatatttgcg
    61381 taccgataga tttcatgatg ctacctcact tccttatttc tatgctcgta gtaaaaaggc
    61441 tcaactcagt aacagctaga aaacgagttt gtcttctaat ttcttgacgc agtcgctgcc
    61501 tggtttgaaa tgcgctctag caagcttttt caaacctagt catcgttgca ggggtaagac
    61561 gatgaaatcg aactcttcga gttaccgaat ctgcctcatt ttcttatttc taaactcggg
    61621 gttcaagatc ccccagacct tctctcactt tcttatttca agatgagagg ctgaaaacct
    61681 ccagtggagg tttttcttgc tttttaattt cagggcaaga agctaaaaag gtccccggac
    61741 cttcttcgct ttctcatttc taggctcagg gtaaaaacct ccaatggagg tttttactcc
    61801 caaaagtcat caaatacagt gatgggtaag tggcgtttgt gttggccttt gtgccaccag
    61861 ttttcaatgg tcgcttgagc ttctgggctg attgttttgc cttctaggta gtcgtcaatc
    61921 tctgcgtagg tgactccaag tgcgacttcg tcagctaggc ctggtttatc ttcttctagg
    61981 tctgccgttg ggattttttc ataaagggct ggctctgcgc caagtttctg caagagctgt
    62041 tttccttggc gtttattgag gcggtaaaga gggagaatat ccgcaccgcc gtcaccaaac
    62101 ttggtaaaga aacctgtgat attttccgcg gcgtggtctg ttccaatgac cgctccgcta
    62161 tgggaaccag caagggcata ctgagcaatc atacggcaac gtgccttgat attccccttg
    62221 ttgaagtctg aaacaggact acctgtcgct tcaactgcag ctgtcatggc atcagctgat
    62281 tccttgatat tcacaaccaa gctgacatct ggctggatga aggctagggc tttttgagca
    62341 tctgcttcat cagcttgcac tccgtatggc aggcggacag cgataaattt gtagctgtcg
    62401 tctcccgttt cagctcgcag ttcttccata gctaattgcg ccaaacgccc tgccaaggtt
    62461 gagtcttgtc ccccagaaat ccctagtaca aaggttttta ggaagggatg ttttttcaga
    62521 tatcttttta agaaatcaat agaacgacgg atttcttcct gggcatcaat cactggtttg
    62581 acacccagct cttggataat cgtttcttgc aaactcattc ttcttctcct tcaccaaggg
    62641 cttccttgcg catcttatca atcaagtcca tcttatcttg ccatacatca cgcgccaaat
    62701 ccactggata gtgctgcgga ttgagcacac gcttatactc atcccacaac ttgtcaaatt
    62761 ccttacgggc ataatcctga atgtcagtca aactaggcaa gttgtaaact aatattcctt
    62821 ctttgaagat atccaccaag agaggaacgg catcaaaatt acgaaccgtc ttcttgatgt
    62881 atgtataggt cggatggaac atcttgattt ctgtcatgtc gctaatatcc acaccatcat
    62941 aagtgatata gtcgccttct gacttgcctt tttcacgact ggtaatgcgc cacacctgct
    63001 tcttacctgg cgtagagact ttttccgcat tattagacag cttaatcgta ttgcgcatct
    63061 gaccagtttc atcttcgatt gcaacaatct tgtaaactgc accaagagct ggctggtcat
    63121 aagctgtaat cagcttggta cccacgcccc agacatcaat cttggccttt tgcatcttga
    63181 gattgaggat ggtattttca tctagatcat tagaagcata aatcttagcc tctgtaaatc
    63241 cagcctcatc cagttgctga cggactttct tagaaatgta ggcaatatcc ccagagtcaa
    63301 tccgcacacc cataaagtta atctgatcac ccagctcacg cgccacctga atggcagctg
    63361 gtacaccgat gcgaagggtg tcataggtat ccacaagaaa gacacaattt ttgtgggtcg
    63421 cagcgtaagc cttgaaagct tcatagtcat tgccataaac ctgtaccaag gcatgggcat
    63481 gggttcccaa aacaggaatg tcaaagagct tacccgcacg cacgttgctg gttccattgg
    63541 cgccaccaat cacagctgcg cgtgttcccc agatggccgc atccatttct tgagcccgac
    63601 gtgtcccaaa ctccatcaag ggttcatctt cgataaccga acgaatacga gctgccttcg
    63661 tcgccaccaa ggtctggtag ttgacgatgt tcaaaagagc cgtttcgacc aactgacatt
    63721 gggctagagg tccttccacc tgcacaatcg gttcattagc aaaaaccaaa tccccttctt
    63781 gggcagaacg aacggtcaac tccaacttga aattgcgaag gtaatccaag aacgccccat
    63841 gataaccaag cgactccaaa taggctatat cactatctga aaaacgcaag tcttcaagat
    63901 agttcacaat tctttccaaa cctgcaaaaa ccgcatagcc gttcttaaaa ggctgttggc
    63961 ggaaatacac ctcaaagacc gccttcttat tgtgaatccc ttggtcaaag taaacctgca
    64021 tcatgttgat ctggtacaag tccgtgtgca atgtcaaact atcatctgga tacatacttt
    64081 tcctacttcc ttagctagaa acccatgaaa attttcaaga actttcatgt attccaataa
    64141 attagtacta ttatatcaca ttttagctgg attgagaaaa gagtaacaag ctattctcca
    64201 ctctccaatt catccatatc ttgttcaaat tttttctgag cccattcgcc atagctctta
    64261 agaccaagat tgccaataaa gacccacgga aggtaaatga cataagtaat gacccaagca
    64321 gacaggtatt taaaattcaa aggattgtgc tgataaattt ctatgttgaa ttgataattc
    64381 tgcaacatca aaagagccgt aatagccaag gttaggaaaa aacaacccaa aatcgtaaaa
    64441 tgaaaacgac tatagtaggt cactcccaga taacgggcac ggttaaaaaa gtaaaatgtc
    64501 cctatgatga taacgattag cagcatatta gaattaaaaa ggcttggtgc taatactgaa
    64561 atgatataag ataggagcga caaagcgatg cagatataga aactttcaga gcccgcttta
    64621 ttgatcagtt gttcttctct ttcgtctagt aattgataat aataaaatct atttttcatc
    64681 ttcttcctcc caaaatagtt ggtctagggt tttccctaaa catctgcaaa tagactggca
    64741 gagcgagaga ctgggattgt attttcccgc ctctatcaaa ccaatagtct ggcgtgtcac
    64801 cccgacagcc tctgccagtt gaccttgtgt taaatcacgc tctacccgag ctaattttaa
    64861 ttttaaattt ttagccacct tcgtcctcct tatagtttta atactcatct actctttaaa
    64921 aatccaaact aacacaagct gtcagctatt tattataacc ccattaattt gcactccagt
    64981 attatcttca gccgaaaatc agtattttag gttgtttgaa ataaacttcc tagtttactc
    65041 ttttattttt atttttattt agcattagct ttagtaggat cttttaaacg agtttttatt
    65101 gcttatattt cttgtttagt ttagttggtt tcatatcact attatataat gcttttggaa
    65161 atgagcctag tataagtcaa aaacgtgttg aatagtgaaa gcaggagcat accaatatga
    65221 ctgatttttt actgtatttt aggtatgcaa aataatacta aatgaaacta aaatgaatac
    65281 ttcttgcttt tcttgagagt gaaaaagtac aataaaatga ttgtagtgat tgccacaaga
    65341 tactgactaa tagacatcgt atttgaagcc acaaatacag gtaaagaata aaaaggaaga
    65401 agttcaattg ctttgataaa caaatcatta ctagtattca tcatcgtatt taatatatta
    65461 gggataaaga gtagtaaagc tactaaaata ccaagccact tttcaatttg tataaataga
    65521 aaatgtaaga taatagaaaa taaaacagga ctaacaaaat tgtattgact acaatagcta
    65581 ctataaaatc taatcctaaa tcacctggaa tacccaattt acttactatg actagtaaca
    65641 caatgccatt caaagcataa ataatggaag ctgagatggc atatagaaaa ttagacaaga
    65701 cgtaaatcat ccgattcttc tgttgattat aaaacaaact taccgtattg tgggaaaaat
    65761 ctctactaat gactcgatta gcatgtataa cagccattaa cataccgagc atagcataaa
    65821 agtttgaaat atgcttaata ccaagcgatg tcccttaagt ctttagaata aagaatgtaa
    65881 gcaatatcgg cggtataaac gagataagca aactgagata tattacgcct gatgaatata
    65941 aatttcttat attttcctta agaaaattca atttatttaa tttcatgatt ataaccttcc
    66001 tttattttga acgatttagg taaatatctt taagcgtctc ttttttagtt tcaaaatcaa
    66061 ctactttaat agagttttcg ttaaaaaatt taaaaagctc actactttga atattcccag
    66121 acatagtaat tctcaatccc tcttcctgaa caatatcccc aaattcttgt ttggtaatga
    66181 aaatgtctct atctgtagct gatgaaaaag ctatctcaaa caagaaatta tgactatctt
    66241 ttcctacttt ttgaaatgtc aaaagcccgt tctccaagaa aagaactctc tcacaaattt
    66301 cttcaatgtc ttctaattta tgactcgata ttaaaattcc cacgttttca tttaaagcta
    66361 atttttttag aaccgctaaa actatttgtg atgattcaat atctaaacca ttagtcggtt
    66421 catctaaaat caatatatca ggttccgtaa cgagagttag aagcaaagct aatttttgtt
    66481 ttgtacccaa agaataggtc tttacttttt tattaataga ctgagtcaaa tctaactctt
    66541 ggatcaaact tctaaatctt tcttgattgt agtcaacacc atataaattt gacaaatatt
    66601 ttaaattctc taaacctgtt ttagataaaa ataattttgg ttcttcgatt aaatacccaa
    66661 cattatcact gcttataata ttacctgaag tcggttgatt attttgaaca agaattttca
    66721 tcaacgtact cttaccaact ccatttctcc ctactagacc aacaatttca cccttgttaa
    66781 gtgcaaaatt tatatctttg agaatgattg aatgaccata atgcttactt acatttttat
    66841 tcctacatta atattcatgg tttactcttt gattcttatt gagtttaaaa agttgcgcct
    66901 ctttttaaca atattatatc atatatctaa cttgatgcaa tatataaatg tcattttgta
    66961 aaattttatt aacaaaaaac tctctacaac aaaataatag agagttttct gcttattaat
    67021 ttttttcaaa atatctaaga catcgcttct ttgtttaatt tgataggctt tgatacctaa
    67081 gctccgactt tatcacttaa aaaataaaaa agacagctaa tctcttgcgc atcaagggat
    67141 ttcgccattc tttaatctcg tttaagtgtt gtatatggag ctttgcataa ttttttaaaa
    67201 ctaaacaact acattaagca cgaactgtga cgctggttcc atcttcctta taaagattga
    67261 taagcccctc tttaagagct cgaaccatgt ctcctgattg aacgggttgt gggactttaa
    67321 tagtcaatag ttccattgga tttggagcgc ggtcgatttt attgcctttg gcatcatgca
    67381 aatcttcaat ataggtttca aaatgacgga aacctggacc ataaaactca acttggtccc
    67441 cttcgttaat gacgtttcgt tgacgaatag ttgctgtttg tgccgcatca tcataagaaa
    67501 ccacttcagc gacaaacttg tactcaggga ttttacgacg agcaccaaac aactgctcat
    67561 tttcagatgg tgtaccatag taaaatcctg tagccagttc acgttgggca accttccaca
    67621 tctcgtccac caagtcttgt ttgatagctt caaacttttc aggactttca agataggcat
    67681 ccacaaccgc cttgtagcag ttggttactg ttaatacata gtgaatagac tccatacgtc
    67741 cttcgatttt tagactgtcc acaccatttt caatcatatc tggaatgtgg tcaatcatag
    67801 acatgtcaac ggctgacatt gaaaattctt ctggaatctc accctgcaaa ctcttacgtt
    67861 ctttcccaaa tggcatatcg taaaggtcgt atttccaacg gcatgactga gaacatccac
    67921 cacggttggc atcacgcata ctctagacgc aattctaatc ttttagactt tgcataataa
    67981 ctgttaaaca gttgaaaatt aacccagttt taacaagtag tcaaaatacc agataaagga
    68041 aaaaaagtct ttaaaatctt aaaaacgcat agtatcaggt attgaacaat cttgatatta
    68101 tgcgttttat tgtgggaaga tttacttcat tttctcttgg aattgagttt tctcctactt
    68161 caaaactaga tgatttgcta tttaaaatag cgttccttat gattttggca acctggatta
    68221 aagcgagaaa aacaaaaggg gcaacttaag ctttctttat attgcttata tgttagtagt
    68281 tttagacaaa caccacataa aataggctta tcctgtatca aagataaggg atacggctca
    68341 aaagggtgat tttctaatga atcatgacac cgataacaag catagtactt tttacaaaca
    68401 tagcattgta aagcaataat atccagcttg ctatggtaat gaatgcatct tgtttgctta
    68461 tccactaaga aaccctgtgc ctctatcatt tccttttcct ttcttaagct cgaactgtga
    68521 cgctggttcc atcttcctta taaagattga tgagtccttc ttttaatgca cgaaccatgt
    68581 ctccagcttg aacaggttga ggcaccttaa tagtcaatag ttccattgga tttggagcgc
    68641 ggtcgatttt attgcctttg gcatcatgca aatcttcaat ataggtttca aaatgacgga
    68701 aacctggacc ataaaactca acttggtccc cttcgttaat gacgtttcgt tgacgaatag
    68761 ttgctgtttg tgtcgcatca tcataagaaa ccacttcagc gacaaacttg tactcaggaa
    68821 ttttacgacg agcaccaaac aactgctcat tttcagatgg tgtaccatag taaaatcctg
    68881 tagccagttc acgttgggca accttccaca tctcgtccac taagttttgt ttgatggctt
    68941 caaacttttc agaactttca agataggcat ccacagccgc cttgtagcag ttggttactg
    69001 ttgatacgta gtgaatagac ttcatacgtc cttcgatttt tagactgtcc acaccatttt
    69061 caatcatatc tggaatgtgg tcaatcatag acatatcaac ggctgacatt gaaaattctt
    69121 ctagaatctc acccttcaaa ctcttacgtt ctttcccaaa tggcatatcg taaaggtcgt
    69181 atttccaacg gcatgactga gaacacccac cacggttggc atcacgcata ctcatgtggt
    69241 ttgaaagagt acaacgtcca gagtatgaaa tacacatagc tccatggaca aaggcttcaa
    69301 tttcaacatc tgtacgtttg cggatttcag ctaattcttt cattgaaacc tcacgtgcta
    69361 aaacgacacg agtcaagcct agctctttcc agaactcaag ggtttcatag ttagtggcac
    69421 tggcttgggt agaaaggtgg atttcaaggc ctggtgcttc agtcgctgca atcataatca
    69481 aggctgggtc agatacgata actgctgcaa tcccgatatc acgcagttta cgaaaccact
    69541 caccagcacc agcttcattt ccttcgtgca taaccatatt agccgctaca tagaccttgg
    69601 caccatactt ggccgcaaac tgcacgcctt cttccatctg ttcgaaagta aagtttcccg
    69661 cacggctacg aagaccatag gcctgaccac cgataaagac agcatctgct ccatactgaa
    69721 cagctacctt tagcttctct aaagtccctg caggtgataa aacctcagga cgttttaatg
    69781 tttttgtcat gatttctcct atttgcaata taaaagtttg acatttttcc ttctcatttt
    69841 atagtaaata aaggataaaa tcaagcctag acggattgtt ttgacaattc taatctttta
    69901 gactttgcat aataactatt aaacagtcga aaattacccc agttttaaca agtagtcaaa
    69961 aagtctaaaa aagtaaaaac cagtatccca agatactgac tcgtaaaaat atataaatta
    70021 gtccttagat gactactccc actctgttgc tcctataatg gaaaaggatt gatacagtct
    70081 agaatatcgc ttttttattt attattctga tataaaactg ttagaattga ttttttgttt
    70141 tatacccaaa tatgaataga gacgtagcta ataagttgaa taacatattt ggaaaaatga
    70201 gaaatagcaa tattactaga acttttagaa acaatcaata tttaaaaaga aaaaagctca
    70261 aagaatttat taaaccatgt tataatagat atagctataa ttttatttat aacaagagga
    70321 tagaaatgac cgaattagaa agaaaaaatc gaaaaattag ctaagaaata ttctgataac
    70381 ttaaacatca aagttcaaga gagagttcgt gaaatggcaa atgataataa gagccattat
    70441 ttgatataca gagttttagg tatttcattt gaagaaggag aaaatatcga tttgtatcaa
    70501 aataaaggtc gttttttata caaatatgct ggttcatttt tagaagaagc tgcagtacta
    70561 tcctttaacg aaaaatttgg tacagaaaat acttaaaaag ttaacattcc taattctgaa
    70621 aatacaaaac ctaagacttt tgaaattgat tgtttagtcg gagaaaaaca cgcatacgaa
    70681 ataaaatggt gggatgcaac tacagatgga gaccatataa ctaaagaaca cactagaata
    70741 aaagttattc ataacaaagg atatatacca attcggttaa tgttctacta tccaaataga
    70801 actcaagcta taaaaattca gcaaacttta gaaacattgt ataacggtat tggagggaaa
    70861 tattattatg gagattctgc ctgggaacat ttaagagcag tgaccggtat tgatttactt
    70921 agtattctaa cagatattgc aaataaaaaa acaggggtaa aatcaaaatg acagtattaa
    70981 aaggagataa cttagaaata ttaaaaacta ttgaatcctc aagtattgat ttaatctata
    71041 tggaccctcc tttctttaca cagaaaaccc aaaaattatc taataacaaa aatattatgt
    71101 attcattcga agatacgtgg acttcgattg aggattacaa agaatttttg tctgtaagat
    71161 tagaagaatg caaaagagtg ctaaaaaata gtggcagtat tttcgttcat tgtgataaaa
    71221 ttgcaaatca tcatattaga ttaattttag ataatatctt tggagcagat atgtttcaaa
    71281 gcgaaattat atggaactat aaacggtggt ctaattcaaa aaagggatta ttgaacaatc
    71341 atcaaaacat ttacttttat tcaaagtcaa aagattttaa atttaataca atttttacag
    71401 agtattcttc tactacaaat atcgaccaaa tactagtaga acgaaaacga aatggaaact
    71461 ctaaaactat atataaagtt gataataatg gtaactatat tctagcaaaa gagaaaaatg
    71521 gagttcccct ttcagatgtt tggaatatac catttcttaa tccaaaagct aaagaaagag
    71581 taggttatcc tacacaaaaa cctattctgt tattagaaca aattataaag attgctactg
    71641 ataaaaatga tatagtttta gacccgttct gtggaagtgg aactacttta gtagcctcca
    71701 agattttgaa tagaaattat atggggattg atttatctga ggaagctatc aatataactc
    71761 agcaacgtct ggaaaatgtt ataaaaacaa gttcaaattt attgaataaa ggaatcgaag
    71821 catatagaac caaaactgag gaagaggaaa acattcttaa attattacag gcaaaaattg
    71881 ttcaaagaaa taaaggaatt gatggttttt tacctaaaca ttttcaaaaa aaaccgatac
    71941 ctataaaaat tcaaaaaaat aatgaatgtc tgaatgagag tatctcttta ttacagaatg
    72001 ctataaactc caaaaaactt gattttggag tagttataaa aactcattca gataatttat
    72061 tatttgattt tgatacgatt cctgaaaata taatagtagt tgaccatttt gaacttacaa
    72121 tcgaaaaatg gttaagtaaa agtcaacagt tactatagtt tgcaaattat ttacagaaag
    72181 aacaaaaaat gctctgaaac actgatttca aggcattttt tgttagttta aaattactcc
    72241 cattcttctt ttccaaacgt acaatatatc caaaaccatt caaaatacta gattctattt
    72301 tttataatat cactaaatcc acctaattat aggacgtttt cagattttta gtcccgtccc
    72361 agtcccagta ccggagaaat attgttttaa tataatatct ctttttgtct tctaagctct
    72421 taaaagcaaa agaacaagta aagagtcaag acaaggataa aaagtccata ttagggcaaa
    72481 taaaaagctt taagacagat gacaaatcta agtcaaataa gaaagaccat agcaaaggtg
    72541 cagagagata aatattggcg gtcttcggac tgcctttatt tttttatcca tttttcaaat
    72601 caaatttatt cagactatat atgcacatat acacttaaat tcatataaaa acatggcttg
    72661 taaaaaatta ctttaatcac aataatcgca tttaaaattg tgatgtttgc aagctaaatt
    72721 acggacttca cttggaagtt ttcccttgta tcttttataa tagatagaaa atttgctggc
    72781 agatgaatat ccaacagatt ctgctatctc ttttataggt agttcagtgt ttaaaagaag
    72841 agtttcagct acattcattc tttttctttg agtgtactct gtaatgcttt gacaatattt
    72901 ttccttaaat aaatttttta atttagtacc actcatttta gatatttttt caagcgtgcc
    72961 ttgatttaca ttcgttgcaa aatgatcatc taagaatctt gctacatctt caagtgcttt
    73021 atcatcatca atttcaattt tatatttttt tctatttaag tatgtgtcaa ttactatact
    73081 tatccattca tttgcctttg ctttaaagaa aaaatcagcg gcaggagcgt ccatcttaca
    73141 atttaatatt tccattgcca ctctttctaa ggcctttgta agtattattt gattcggttg
    73201 aagcaaggtt gaataaaaag attctggatt aatgttaata gatgctaaat gtttttctat
    73261 tagctctttt ttaaaaccaa tggaaacagc aagataacaa caattctcgt gtaataaaaa
    73321 aacaaaatca tcttttatat tatcaaaatc aaaagtacat aaagagtttg cggtaatagt
    73381 ttgatacgga ttaaactttt ctccgtttgc actgacaatg taacttgaat aaattgaaac
    73441 atagtctgac atactataag tgctattttg aactacttcc tctttgatat aaaaatcatg
    73501 tatatcgata atgaagctgc ctccttcata aaaccaatat aggccttctg cataagttga
    73561 attctctttt ttccaacaaa atgtatgacc tgcacttgaa tacttctcat tgtttttaca
    73621 ttcatctact ttcatataat cttttacaaa atcataatat gacataacac actatccctt
    73681 ttagacaata ttccaattag ccttattaat tcaaaactat tgtattagta attataacag
    73741 atgtataata gaaaagcaat gatagatatt atcaattaag cgaatttata tctaaaaggg
    73801 atattaaaga aaggagatat gcttatgaag atttacaaaa aactatttgc ttatgtccaa
    73861 gataagaaat atcttggggt tttggccata attttttctg ctatatctgc tgtacttaca
    73921 gtatatggat attatttaat ctacaaattt ctagataagt taataattaa ttcaaactta
    73981 tccggtgcag agagtatagc attaaaatct gttattacac taacaagtgg agcgatattt
    74041 tattttgtct caggaatgtt ttcacatatc ttgggattta ggcttgaaac aaatttaaga
    74101 aaaaggggaa tcgatggtct ggaaaaagca agttttaggt tctttgactt aaatccatct
    74161 ggtcaaataa gaaagatcat agatgataat gcagcacaaa ctcatcaggt ggtagctcac
    74221 atgattccag atagctctca ggcaatagtc acacctatac ttgtccttgt acttggcttt
    74281 atagtaagta taagagttgg cataattttg cttgctctta ctataattgg tggcttaatt
    74341 ttaggggcaa tgatgggcga gcaagaattt atgaagatat accaagaatc cctatctaaa
    74401 ctaagtgctg aaactgttga gtacgtgaga ggaatgcaag ttgtaaaaat atttaaagca
    74461 aatgtagagt cttttaaaag cttttataag gcgataaaag attactcaaa gtatgcttat
    74521 gattattccc tatcttgtaa aaggccttat gttttgtatc aatggttatt ttttggactg
    74581 attgcaattt taattattcc tatagtttat tttatgacta gcttagctag cgcagaggtg
    74641 attttacttg agcttatcat gattttattt ttatcaggag ttctctttgt ttcattcatg
    74701 agaatgatgt ggtactccat gtatatttct caaggaaatt atgcagtaga tactttagag
    74761 gcgctttacg aagatatgca aaaagacaaa ttagtgcatg gtaatgtcaa taattttaaa
    74821 aactataata tagaatttga gaatgttagc tttgcttata atgataaagc tatcattgaa
    74881 aatttatcct ttaatttaga agaaggaaag tcctacgcac ttgtcggttc atctggatca
    74941 ggcaaatcaa cagtagcaaa acttatatca ggtttttaca atgttaataa aggaagcata
    75001 aagataggcg ggatagcaat aagtgaatat tctgacgaag ccttaattaa agccatttcc
    75061 tttgtttttc aagattcaaa attattcaag aagagcattt atgataatgt agctttagct
    75121 aataaagatg cgacgaaaga tgacgttatg agagccttaa aattagcagg atgcgattta
    75181 atattagaca aattcccaga aagagaaaat acaatcatag gctcaaaagg tgtttattta
    75241 tccggtggag aaaaacaaag aattgcaatt gctagagcaa ttttaaagga ttccaaaatt
    75301 attattatgg atgaagcatc agcatctatt gacccagata acgagtttga attgcaaaaa
    75361 gcttttaaaa atcttatgaa ggataaaaca gttatcatga ttgcacacag gctatctaca
    75421 attaaagacc ttgatgaaat tattgtcatg gatagtggaa aaattataga aagagggtct
    75481 gacaaagaat taatgtcaaa agatacaagg tataagagcc tgcaagagat gtttaacagt
    75541 gcgaatgaat ggagggtttc aaatgaaaga gttttataaa aaaagatttg ctcttacaga
    75601 tggaggagca agaaatttaa gtaaagcaac actggcttca tttttcgttt attgtataaa
    75661 catgcttcct gccatattac ttatgatttt tgctcaggaa gttttggaaa atatgggcaa
    75721 aagcaatggc ttttatatag tattctcagt tttgactttg atagcaatgt atattttgct
    75781 ttctatcgaa tacgataaat tatataacac aacctatcaa gaaagtgcag atttaagaat
    75841 aaggacagcg gagaatttat caaaattacc tctatcttac ttttctaaac atgacatttc
    75901 cgacatttca caaacaatca tggctgatat tgaaggcata gagcatgcaa tgagccactc
    75961 aataccaaag gtgggcggca tggtactgtt tttcccatta atatctgtaa tgatgctagc
    76021 gggcaatgtc aagatgggtt tagctgtaat tattccatct attttaagct ttatatttat
    76081 acctttatct aaaaaatatc aggttaatgg acagaataga tattatgatg tcttaagaaa
    76141 aaactcagaa agctttcaag aaaatatcga aatgcaaatg gagattaaag catataattt
    76201 atcgaaggat attaaagatg acttatataa aaaaatggaa gatagtgaga gaatacactt
    76261 aaaggcggaa gtaactacaa ttttaacttt gtctatatct tcaatattta gctttatatc
    76321 tcttgctgtt gtgatatttg tcggcgtaaa tctaattatt aataaagaga taaattctct
    76381 ctaccttata ggatatttac tagctgctat gaagataaaa gactctttag atgcatctaa
    76441 agagggcttg atggaaatat tttatttatc gcccaaaata gaaagattaa aagaaattca
    76501 aaatcaagat ttacaagaag gcgatgacta tagcttaaaa aaatttgata ttgatctaaa
    76561 agatgttgag tttgcctaca ataaagacgc aaaagtttta aatggtgtaa gttttaaagc
    76621 taagcaggga gaggtcactg ctttggtagg tgcaagtggc tgcggtaaaa caactatctt
    76681 gaaacttata tcaagacttt atgattatga caagggacaa atcttaatcg atggcaaaga
    76741 tataaaagaa atatcaacag aatctctttt tgataaggtc tctatagttt tccaagatgt
    76801 ggttctcttt aatcaaagcg ttatggaaaa tattagaatc ggtaagcaag atgcaagtga
    76861 cgaagaggtt aaaagagcag caaaacttgc aaattgcaca gattttatag aaaaaatgga
    76921 taaaggtttc gatacagtta ttggtgaaaa cggagctgag ctatcaggag gagaaagaca
    76981 aagattatca atagccagag ccttcttaaa agatgcgccg atattgatct tagatgagat
    77041 aacagcaagc cttgatgtta acaacgagaa aaagattcaa gagtctttaa ataatttagt
    77101 taaagataaa actgttgtaa tcatttcaca tagaatgaaa tccatagaaa atgcagacaa
    77161 gatagtagtt cttcaaaacg gaagagtaga aagcgaaggt aagcatgaag agcttttaca
    77221 aaaatcaaaa atttacaaaa atttaataga aaagacaaaa atggcagaag aatttattta
    77281 ttaggaggac tacaatggat aataaaaaat taaaagtaaa agatttagta agtatcggtg
    77341 tttttggcgt aatttatttt gccttcatgt ttggagttgg tatgatgggc ttgattccaa
    77401 tattgttctt aatatacccg acagtattag ccatagttgc aggaactgtt gttatgttat
    77461 ttatggctaa ggttcaaaag ccatgggcac tatttatatt tggtatgata tcaccacttg
    77521 tgatgtttgc agctggtcat acctacgtag ttgtggtttt atcacttata gtaatgataa
    77581 tagcagaatt aattagaaag attggtaatt ataattcatt taaatacaat atgctttctt
    77641 atgcaatctt cagcacatgg atatgtagct ctttaatgca aatgctttta gcaaaagaaa
    77701 aatatatgga gtggtctttg atgacgatgg gaaaagatta tgttgatgta ttagaaaagt
    77761 taataactta tcctcacatg gctttaatag ccttaggtgc tttcttagga ggaattcttg
    77821 gagcatatat aggcaaggct ctattgaaaa aacacttttc aaatggatta tattgtgtgg
    77881 gatactttac tccttgccta attttatggt gttatctgaa ttaaacccta tagttaagat
    77941 gtttttgagt atacctattg ttattagaat gtttatttta ccatttatgg cagcaagctt
    78001 tatgataaag acctcggatg taggcgcaat aatttcatcg atggataagc ttaagatttc
    78061 aaagaatgta tccataccta ttgcggttat gtttagattc ttcccatctt ttaaggagga
    78121 gaagaaaaac atcaaaatgg ctatgagagt aagagggata aattttaaaa acccagtcaa
    78181 atatcttgaa tatgtttctg tgccactact cattatatca tctaatatat cagatgacat
    78241 tgcaaaagcg gcagaaacaa aggcaataga aaatccaatt gccaagacca gatacattcg
    78301 cgtaaagata cagctaattg attttgttta tgttttagcg gttgctggac ttattgtggg
    78361 aggcttaata tggttgaaat aaaaaattta agtcttgatt atggtgaaga gcatatatta
    78421 gatgatatat cactatccat agccgaggga gagtgcgtgc tatttacagg aaaaagtgga
    78481 agtggtaagt catctttaat aaattcaatc aatggactag ctgtaaggta tgataacgca
    78541 aagacaaagg gcgaaataat tattgatggt aagaatataa aaaatttgga actttatcaa
    78601 atctcaatgc ttgtttcaac tgtttttcaa aatcctaaga catatttttt taatgtcaat
    78661 acgacattag aattattatt ttatttggaa aatatcggtc ttgcaagaga agagatggac
    78721 aggcgtttga aggatatact tgagatattc ccgataaaaa atcttttgaa cagaaatata
    78781 tttaatctat ccggcggtga aaaacaaatt ctttgcattg cagcttctta tatagcaggt
    78841 acaaagatta tagttatgga tgagtcttca tcgaatttag atattaaaag cataagtgtt
    78901 ttggcaaaga tgctaaagat attaaaagag aaaggcataa gcataattgt tgcagagcat
    78961 agaatttatt atttgatgga catagttgac cgtgtatttt taatagataa aggaaagctt
    79021 aaaaaaactt atactagaag tgaattttta aagctagata aaaatgaatt aaatgcttta
    79081 agtttaagag ataaagaatt aagtaaatta aaagttcctt atttaaaaga aggtggagag
    79141 tatcagataa aaaatcttag ctacaaattt actgatgatg agtgtttaag cttaaaagat
    79201 atttcgttca agcttgggaa aatttatggc ataataggat ccaacggacg aggaaaatca
    79261 acgcttttaa gatgtttaat aggtcttgag aaaaaatcaa aagaagaaat ttattttaag
    79321 ggagagaagc tatctaaaaa agaaagactc aaaaactctt cacttgttat gcaagatgta
    79381 aatcatcaat tattcacaga tgaagtattc aacgagctta gattaggagt aaagaatttt
    79441 gatgaagaaa aggcgaaaat cattttaaaa gatttaggcc tggacgaatt tattgaaagg
    79501 catccgatga gtttatcagg agggcaaaag caaaggcttg caatagcatc tgttatgtgc
    79561 aagacttctc catttatctt ttttgacgaa ccttcaagtg gtatagatta ttccaatatg
    79621 ataaaaatat ctgaactgat taataagtat aaaaccatgg ataaaataat ttttattgtt
    79681 tcccatgata tagaattttt aaatgaagtg gcagatgaaa tttttgaatt gtaaaataga
    79741 gtctgttaaa catgtctata gaactggtaa attgtctaaa aatttaaaag atactaacac
    79801 atattgatat ttattagaaa attaacttca ggatttttac agtatttata aactttataa
    79861 tatgtattta tagtattaat aatttccata tctttaggga aattatttat ttacttacca
    79921 taattaaaga gatgagagat aaatatcggc agtcttcgga ctgcctttat ttttttactt
    79981 aatttaatgc agtaaatcat acgattgaaa ggaatctgcg ttatatgcta taattaggta
    80041 gttaatctat acatttagaa aggtaaaata tagaattgga gaatgatatg ggattttcat
    80101 ataataaatt gtaacc
//
LOCUS       DAVBCV010000003        79966 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBCV010000003 DAVBCV010000000
VERSION     DAVBCV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 79966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79966
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.003"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..722
                     /locus_tag="VQ516_000156"
     CDS             <1..722
                     /locus_tag="VQ516_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET2510675.1"
                     /translation="KKDAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQS
                     DWKVQYTRCLDIIFPELDKIVEKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTAS
                     KIQDILSVAPRSIGTTSPAREFEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGN
                     RLEAVILAEIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQ
                     AAKACPRFSPAFKAYLKTKLE"
     gene            complement(1023..2912)
                     /locus_tag="VQ516_000157"
     CDS             complement(1023..2912)
                     /locus_tag="VQ516_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2510676.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSLFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            3050..4615
                     /locus_tag="VQ516_000158"
     CDS             3050..4615
                     /locus_tag="VQ516_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2510677.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKKPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMCKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4615..5379
                     /locus_tag="VQ516_000159"
     CDS             4615..5379
                     /locus_tag="VQ516_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2510678.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQARPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(5578..5793)
                     /locus_tag="VQ516_000160"
     CDS             complement(5578..5793)
                     /locus_tag="VQ516_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510679.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(6664..7914)
                     /gene="ilvA"
                     /locus_tag="VQ516_000161"
     CDS             complement(6664..7914)
                     /gene="ilvA"
                     /locus_tag="VQ516_000161"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2510680.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7964..8320)
                     /locus_tag="VQ516_000162"
     CDS             complement(7964..8320)
                     /locus_tag="VQ516_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2510681.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(8558..8794)
                     /locus_tag="VQ516_000163"
     CDS             complement(8558..8794)
                     /locus_tag="VQ516_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510682.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(8901..9923)
                     /gene="ilvC"
                     /locus_tag="VQ516_000164"
     CDS             complement(8901..9923)
                     /gene="ilvC"
                     /locus_tag="VQ516_000164"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2510683.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVVEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9989..10465)
                     /gene="ilvN"
                     /locus_tag="VQ516_000165"
     CDS             complement(9989..10465)
                     /gene="ilvN"
                     /locus_tag="VQ516_000165"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2510684.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(10458..12158)
                     /locus_tag="VQ516_000166"
     CDS             complement(10458..12158)
                     /locus_tag="VQ516_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2510685.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(12359..14026)
                     /locus_tag="VQ516_000167"
     CDS             complement(12359..14026)
                     /locus_tag="VQ516_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2510686.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(14029..14394)
                     /locus_tag="VQ516_000168"
     CDS             complement(14029..14394)
                     /locus_tag="VQ516_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2510687.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(14551..14739)
                     /locus_tag="VQ516_000169"
     CDS             complement(14551..14739)
                     /locus_tag="VQ516_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2510688.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(14855..15031)
                     /locus_tag="VQ516_000170"
     CDS             complement(14855..15031)
                     /locus_tag="VQ516_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2510689.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(15142..15291)
                     /locus_tag="VQ516_000171"
     CDS             complement(15142..15291)
                     /locus_tag="VQ516_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2510690.1"
                     /translation="MDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
                     EIVNV"
     gene            complement(15603..17147)
                     /locus_tag="VQ516_000172"
     CDS             complement(15603..17147)
                     /locus_tag="VQ516_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2510691.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            17360..17662
                     /gene="gatC"
                     /locus_tag="VQ516_000173"
     CDS             17360..17662
                     /gene="gatC"
                     /locus_tag="VQ516_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2510692.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            17662..19128
                     /gene="gatA"
                     /locus_tag="VQ516_000174"
     CDS             17662..19128
                     /gene="gatA"
                     /locus_tag="VQ516_000174"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2510693.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            19128..20570
                     /gene="gatB"
                     /locus_tag="VQ516_000175"
     CDS             19128..20570
                     /gene="gatB"
                     /locus_tag="VQ516_000175"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2510694.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            20699..21259
                     /gene="efp"
                     /locus_tag="VQ516_000176"
     CDS             20699..21259
                     /gene="efp"
                     /locus_tag="VQ516_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2510695.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            21281..21670
                     /locus_tag="VQ516_000177"
     CDS             21281..21670
                     /locus_tag="VQ516_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2510696.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            21663..22085
                     /gene="nusB"
                     /locus_tag="VQ516_000178"
     CDS             21663..22085
                     /gene="nusB"
                     /locus_tag="VQ516_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2510697.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(22134..22511)
                     /locus_tag="VQ516_000179"
     CDS             complement(22134..22511)
                     /locus_tag="VQ516_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2510698.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(22852..23037)
                     /gene="briC"
                     /locus_tag="VQ516_000180"
     CDS             complement(22852..23037)
                     /gene="briC"
                     /locus_tag="VQ516_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2510699.1"
                     /translation="MTGTETFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(23196..23963)
                     /locus_tag="VQ516_000181"
     CDS             complement(23196..23963)
                     /locus_tag="VQ516_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2510700.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(23960..24826)
                     /locus_tag="VQ516_000182"
     CDS             complement(23960..24826)
                     /locus_tag="VQ516_000182"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2510701.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(24863..26230)
                     /gene="accC"
                     /locus_tag="VQ516_000183"
     CDS             complement(24863..26230)
                     /gene="accC"
                     /locus_tag="VQ516_000183"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2510702.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(26242..26664)
                     /gene="fabZ"
                     /locus_tag="VQ516_000184"
     CDS             complement(26242..26664)
                     /gene="fabZ"
                     /locus_tag="VQ516_000184"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2510703.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(26661..27146)
                     /locus_tag="VQ516_000185"
     CDS             complement(26661..27146)
                     /locus_tag="VQ516_000185"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2510704.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(27149..28384)
                     /gene="fabF"
                     /locus_tag="VQ516_000186"
     CDS             complement(27149..28384)
                     /gene="fabF"
                     /locus_tag="VQ516_000186"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2510705.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(28406..29137)
                     /gene="fabG"
                     /locus_tag="VQ516_000187"
     CDS             complement(28406..29137)
                     /gene="fabG"
                     /locus_tag="VQ516_000187"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2510706.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(29171..30091)
                     /gene="fabD"
                     /locus_tag="VQ516_000188"
     CDS             complement(29171..30091)
                     /gene="fabD"
                     /locus_tag="VQ516_000188"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2510707.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(30084..31058)
                     /gene="fabK"
                     /locus_tag="VQ516_000189"
     CDS             complement(30084..31058)
                     /gene="fabK"
                     /locus_tag="VQ516_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2510708.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(31177..31401)
                     /locus_tag="VQ516_000190"
     CDS             complement(31177..31401)
                     /locus_tag="VQ516_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2510709.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(31461..32435)
                     /locus_tag="VQ516_000191"
     CDS             complement(31461..32435)
                     /locus_tag="VQ516_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2510710.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANNRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(32435..32869)
                     /locus_tag="VQ516_000192"
     CDS             complement(32435..32869)
                     /locus_tag="VQ516_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2510711.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(32963..33748)
                     /locus_tag="VQ516_000193"
     CDS             complement(32963..33748)
                     /locus_tag="VQ516_000193"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2510712.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            34073..35437
                     /locus_tag="VQ516_000194"
     CDS             34073..35437
                     /locus_tag="VQ516_000194"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2510713.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            35440..35811
                     /locus_tag="VQ516_000195"
     CDS             35440..35811
                     /locus_tag="VQ516_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2510714.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            36025..37299
                     /gene="serS"
                     /locus_tag="VQ516_000196"
     CDS             36025..37299
                     /gene="serS"
                     /locus_tag="VQ516_000196"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2510715.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(37324..38223)
                     /locus_tag="VQ516_000197"
     CDS             complement(37324..38223)
                     /locus_tag="VQ516_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2510716.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(38333..38881)
                     /locus_tag="VQ516_000198"
     CDS             complement(38333..38881)
                     /locus_tag="VQ516_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2510717.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(39099..40421)
                     /locus_tag="VQ516_000199"
     CDS             complement(39099..40421)
                     /locus_tag="VQ516_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2510718.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(40512..40600)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(40764..43100)
                     /locus_tag="VQ516_000200"
     CDS             complement(40764..43100)
                     /locus_tag="VQ516_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2510719.1"
                     /translation="MNKKILETLEFDKVKALFDPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(43204..43752)
                     /locus_tag="VQ516_000201"
     CDS             complement(43204..43752)
                     /locus_tag="VQ516_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2510720.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIFYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(43749..44051)
                     /gene="zapA"
                     /locus_tag="VQ516_000202"
     CDS             complement(43749..44051)
                     /gene="zapA"
                     /locus_tag="VQ516_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2510721.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            44138..45019
                     /locus_tag="VQ516_000203"
     CDS             44138..45019
                     /locus_tag="VQ516_000203"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2510722.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            45023..45637
                     /gene="lepB"
                     /locus_tag="VQ516_000204"
     CDS             45023..45637
                     /gene="lepB"
                     /locus_tag="VQ516_000204"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2510723.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            45916..48282
                     /locus_tag="VQ516_000205"
     CDS             45916..48282
                     /locus_tag="VQ516_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2510724.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTHLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(48330..49613)
                     /locus_tag="VQ516_000206"
     CDS             complement(48330..49613)
                     /locus_tag="VQ516_000206"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2510725.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(49807..50166)
                     /locus_tag="VQ516_000207"
     CDS             complement(49807..50166)
                     /locus_tag="VQ516_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2510726.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(50465..51094)
                     /locus_tag="VQ516_000208"
     CDS             complement(50465..51094)
                     /locus_tag="VQ516_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2510727.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPTVSPSQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYPSYNFKVHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(51139..51378)
                     /locus_tag="VQ516_000209"
     CDS             complement(51139..51378)
                     /locus_tag="VQ516_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510728.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(51528..51794)
                     /locus_tag="VQ516_000210"
                     /pseudo
     CDS             complement(51528..51794)
                     /locus_tag="VQ516_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(51819..52127)
                     /locus_tag="VQ516_000211"
     CDS             complement(51819..52127)
                     /locus_tag="VQ516_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2510729.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLRLQIAS
                     "
     gene            complement(52052..52294)
                     /locus_tag="VQ516_000212"
     CDS             complement(52052..52294)
                     /locus_tag="VQ516_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2510730.1"
                     /translation="MSLADLLEELEAAKDSKKARPMEAYMRHQFSFLGIAVPERNKLY
                     KNIYQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(52295..52927)
                     /locus_tag="VQ516_000213"
     CDS             complement(52295..52927)
                     /locus_tag="VQ516_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2510731.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(52941..53936)
                     /locus_tag="VQ516_000214"
     CDS             complement(52941..53936)
                     /locus_tag="VQ516_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2510732.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLYDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLDQEEEIIEKERKRIARDLHDTVSQELFAAHIILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(53933..54631)
                     /locus_tag="VQ516_000215"
     CDS             complement(53933..54631)
                     /locus_tag="VQ516_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2510733.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(54709..55719)
                     /locus_tag="VQ516_000216"
     CDS             complement(54709..55719)
                     /locus_tag="VQ516_000216"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2510734.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(55703..56710)
                     /locus_tag="VQ516_000217"
     CDS             complement(55703..56710)
                     /locus_tag="VQ516_000217"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2510735.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRSFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(56697..57650)
                     /gene="mvaD"
                     /locus_tag="VQ516_000218"
     CDS             complement(56697..57650)
                     /gene="mvaD"
                     /locus_tag="VQ516_000218"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2510736.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVIADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YSVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(57632..58510)
                     /gene="mvk"
                     /locus_tag="VQ516_000219"
     CDS             complement(57632..58510)
                     /gene="mvk"
                     /locus_tag="VQ516_000219"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2510737.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(58632..58961)
                     /locus_tag="VQ516_000220"
     CDS             complement(58632..58961)
                     /locus_tag="VQ516_000220"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2510738.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
     gene            59623..59959
                     /locus_tag="VQ516_000221"
                     /pseudo
     CDS             59623..59959
                     /locus_tag="VQ516_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
     gene            59859..60446
                     /locus_tag="VQ516_000222"
     CDS             59859..60446
                     /locus_tag="VQ516_000222"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510739.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSRGYIGVY"
     gene            60489..61721
                     /locus_tag="VQ516_000223"
     CDS             60489..61721
                     /locus_tag="VQ516_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2510740.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(61839..62795)
                     /gene="cbpC"
                     /locus_tag="VQ516_000224"
     CDS             complement(61839..62795)
                     /gene="cbpC"
                     /locus_tag="VQ516_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2510741.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(62893..63582)
                     /locus_tag="VQ516_000225"
     CDS             complement(62893..63582)
                     /locus_tag="VQ516_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2510742.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(63594..65018)
                     /gene="gndA"
                     /locus_tag="VQ516_000226"
     CDS             complement(63594..65018)
                     /gene="gndA"
                     /locus_tag="VQ516_000226"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2510743.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(65094..66488)
                     /gene="mapZ"
                     /locus_tag="VQ516_000227"
     CDS             complement(65094..66488)
                     /gene="mapZ"
                     /locus_tag="VQ516_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2510744.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(66501..67658)
                     /locus_tag="VQ516_000228"
     CDS             complement(66501..67658)
                     /locus_tag="VQ516_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2510745.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(67743..68123)
                     /gene="rnpB"
                     /locus_tag="VQ516_000229"
     ncRNA           complement(67743..68123)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQ516_000229"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(68144..68473)
                     /gene="gpsB"
                     /locus_tag="VQ516_000230"
     CDS             complement(68144..68473)
                     /gene="gpsB"
                     /locus_tag="VQ516_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2510746.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(68543..69070)
                     /locus_tag="VQ516_000231"
     CDS             complement(68543..69070)
                     /locus_tag="VQ516_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET2510747.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            69136..69732
                     /gene="recU"
                     /locus_tag="VQ516_000232"
     CDS             69136..69732
                     /gene="recU"
                     /locus_tag="VQ516_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2510748.1"
                     /translation="MINYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            69729..71888
                     /locus_tag="VQ516_000233"
     CDS             69729..71888
                     /locus_tag="VQ516_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET2510749.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(72055..77358)
                     /locus_tag="VQ516_000234"
     CDS             complement(72055..77358)
                     /locus_tag="VQ516_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HET2510750.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATANENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLFSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVFLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(77659..79641)
                     /locus_tag="VQ516_000235"
     CDS             complement(77659..79641)
                     /locus_tag="VQ516_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2510751.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT        23293 a        18190 c        14785 g        23698 t
ORIGIN      
        1 ataagaaaga cgctcatggc atcgctctta aacttctttc tgaccccaat cgtgaacaat
       61 ttcaacacga taatcggcaa gtagaactaa aaatactggc tagacatatt catcgtctca
      121 agaaaaaaca gtctgattgg aaagtacaat acactcgttg tcttgatatc atctttcctg
      181 agttggataa aatcgttgaa aagcattcag aatataccta ccaactcttg acatgctacc
      241 ctaatcctca gaaaaggctt gaggcaggat ttgataagct gatagaaatt aagcgattga
      301 ccgcttctaa aattcaggat atcctctcag ttgcacctcg ttctatcgga acaacatctc
      361 ctgctcgtga attcgaaatc atcaaacatt acaagaggct cattgacaag gcggaaacat
      421 gtgtcaatga cttgatggct gagttcaact cggtcatcac gacggtcact gggattggga
      481 atcgtttaga ggcggtcatt ttagccgaga ttcgaaatat tcatgccttt gataatcctg
      541 ctcaattaca agctttcgct ggactggatt cttctattta tcagtcaggt cagattgatt
      601 tagctggaag aatgatcaaa cggggttccc ctcatctgcg gtgggcactc atacaagctg
      661 ccaaagcatg ccctcgcttt tcacctgctt ttaaggccta tcttaagact aagttagaat
      721 aaggaaaaca ttacaatgta gccatcatcc accttgcaaa aaaacttatc cgaaccctgt
      781 tttatatcct aaaaaagagc tgccatttga cgaacaaaaa gtgatgtaat ttctgaaatt
      841 aacacaggat tacctttgaa aaaatggact ttttcaaggg ctttttgccg tgctctaaaa
      901 tcatcgccaa atcatcaaat tgaaataaga aatagtttag ataaaactat tgacttttct
      961 tatagtttgt cttttttcta tcttccatta tatcatactt tttcagtcta tggtagctct
     1021 ctttaaaagg caccgatact gccgccacct ccgcctccag agaagccacc accagaactt
     1081 ccacttccag aagatacaga gtaggtgctt gctgtatttg cgacactagc ataatggctc
     1141 atttgcgcgc ttgaatgata aaacagactg tgccagccat aagctacata gagattgata
     1201 tctggatttt ccacttgaat ctgatgaacc ttcatcaaat gactaacctt gtccgcatag
     1261 ccaaataagg tcgcatagac caagaggcga ttccagacca caatactttc cagttcagcc
     1321 tgatccaatc gtgcaatctc acgcaacata ttttcaaaac tggtccagag atagtagacc
     1381 tcagctcccg cttcatttag aacaccatca cgattatcta gtcgaagctt ccaataatag
     1441 aaaacagaca aaactaaccc tagaaaacca agtattggca aagggaggta aagatagcca
     1501 tgaacgtcta aactgtacaa gaacaaacca aatccgataa ataggggcag gatagtcaag
     1561 gcacccatac ccacttgcaa ggccttttcc ccaccagtta aaggacgata ataatctggg
     1621 agcccccaga aggaaactcg ttttctcact ccttcttgca tctggttcaa tacctcttca
     1681 aaagaagatt tgagttgaag ccctcttgct tgaatccgtt tttcatcaga aactttggct
     1741 ctacgataaa gactatcaga taccttgtaa tccgcaaaca aattggaaag agtttcttct
     1801 tttttacctg aaaaagctag atttaggcag tctttctcaa agcttgacaa accatcttct
     1861 tttactagcc tcaaaccaac tgcatctcct tctgaaatga tagagacatt cccacggtct
     1921 atcacatcta gcaaggtagc ttgaataagt tgatcaaagg tgaattttcc agctcccttg
     1981 accaagggac tcacttcctc caaggaggtc gagtagactg cttctgataa aaccataggc
     2041 tctaattcca ttggtggttc atagagacga tgatttttgg catatttgac tgaaggagtg
     2101 gtctttcttc tataaataaa atagaagcag acactcaata acaaggagat agaaaggatc
     2161 gaagggagga cccaagtaac aagttgttta ctctgatctt tttctctaac aatcgagtct
     2221 tctatcttat taaactcttc taaacgattc cctttcaatc cctgatccct agcgctagca
     2281 aaatcggtcc gaggccaata ggcatgcaac tcaactccac gcttagccgg aagattgtct
     2341 aaacggatag tataatcaag gttactcttt tcaatcgttc cctctctaaa aagtttccct
     2401 gtatggaaaa agagtttttc agctcccttg tctcccctta catgaaattc aaacttttca
     2461 atagactctg aactatctgt cagaggttgc caatttaatt cagcgatatc atcataaagg
     2521 aaaagtaaat tttttaagtt ccagacgagg tcaacttcaa ctatgtcgcc ctcctgacct
     2581 ggattataga ctctcacagt ataaccatcc gcttcttctg ttacttcgct agtcacatct
     2641 gctagttctg caccgttttt cgcggcctga acctttggat gagggtcaat gtcaaacccg
     2701 ctaggcatct taccagcacg tccaagtccc acgatttgtc ccttaaagtc ctcctcaaac
     2761 tggtaaacta tcttctgtct aaactctgcc gtattatctg catgaatata caaatcccct
     2821 tgataagagt ttatcttgaa atcaatggca aaaacagaga gtggaagaag gcaaaacaag
     2881 cctaacacca gtaagaaaaa agtttttttc atcaactaag cccccttttt atctttctta
     2941 tcattatagc attttttcgt aagtaaaggc ttacttatgt tgaaaaatta cgcatttttc
     3001 gataaaatag gagacagtta ttttttaata gatcagaata ggagaaatca tgagaaaaat
     3061 atacttatct attttcacaa gtctcttgct gatgctagga cttgtcaatg ttgctcaagc
     3121 cgatgaatat ttacgcatcg ggatggaagc agcatatgct ccctttaact ggacccagga
     3181 tgatgatagc aacggagctg tcaaaatcga tgggaccaat cagtatgcca acggatacga
     3241 tgttcaaatc gccaagaaaa tcgctaagga cttaggtaaa aaacctttgg ttgttaaaac
     3301 caagtgggaa ggtctagtcc ctgcccttac ttctggtaag attgacatga ttatcgcagg
     3361 tatgagtcca actgcagaac gcaaacaaga aattgccttt tcgagcagtt actacactag
     3421 cgaaccagtt ttgcttgtca aaaaagattc tgcctacgca agtgctaaat ctttggatga
     3481 ctttaacggt gcaaaaatca cttctcaaca aggggtctac ctctacgact tgattgcaca
     3541 aatcccaggt gctaaaaaag aaacagccat gggagacttc gctcaaatgc gacaagctct
     3601 tgaggctggt gtcattgatg cttatgtttc tgaacgtcca gaagcactga ctgctgaagc
     3661 tgcgaactct aagttcaaga tgattcaagt agaacctggt ttcaaaactg gggaagaaga
     3721 tacagctatc gctatcgggc ttcgtaaaaa tgacaatcgt attagccaaa tcaatgccag
     3781 cattgaaacc atttcaaaag atgaccaagt tgccttgatg gatcgtatga tcaaggaaca
     3841 acctgccgaa gctacaacaa ctgaagagac tagcagtagt ttctttagcc aagtcgctaa
     3901 aattctttct gaaaactggc aacaactctt gcgtggtgct ggtatcactc ttttaatctc
     3961 tatcgtcgga accatcatag gtctcattat tggacttgcc attggtgtct tccgtactgc
     4021 tcctctctct gaaaacaaag ccatttatgg ccttcaaaaa ctagtcggct gggttctcaa
     4081 tgtctacatt gaaattttcc gtggtacgcc aatgattgtt caatcgatgg ttatctacta
     4141 tggaactgcc caagctttcg gtatcaacct tgaccgtaca ctagctgcta tcttcatcgt
     4201 ttcaatcaat accggtgcct acatgactga aattgtccgt ggtggtatcc tggcagttga
     4261 caagggacaa tttgaagctg cgactgctct tggtatgact cataaccaaa ccatgtgtaa
     4321 gattgtccta cctcaggttg tccgcaatat cctaccagca actggtaatg aatttgtcat
     4381 caatatcaaa gatacatctg tattgaacgt tatctctgtt gtcgaacttt atttctcagg
     4441 aaataccgtg gcaacacaaa cctatcaata cttccagaca tttacaatca tcgccgtgat
     4501 ttactttgtc ctcaccttca ccgtaacacg tatcctacgc tttatcgagc gcagaatgga
     4561 catggatacc tacactacag gtgctaacca aatgcaaacg gaggatttga aataatgaca
     4621 caagcaatcc ttgaaattaa acacctcaaa aaatcctatg gacaaaacga agtgctaaaa
     4681 gacatttctc tcactgtcca caagggagaa gttatttcta tcatcggaag ctctggaagt
     4741 gggaaatcaa ccttcctacg ctccattaac ctacttgaaa cgccaactga tggacaaatc
     4801 ctttatcatg gacaaaacgt cctcgaaaaa ggctatgacc tcacgcaata ccgtgaaaag
     4861 ttagggatgg ttttccaatc ctttaacctc tttgaaaatc tcaacgttct tgaaaacaca
     4921 atcgtcgctc agacaactgt ccttaaacgc gaacgcacag aagctgaaga aattgccaaa
     4981 gaaaacctgg aaaaggtcgg catgggagaa cgctactggc aagcgagacc caaacaactc
     5041 tcaggtggtc aaaaacaacg tgtggccatc gctcgtgccc tttcaatgaa tcctgacgcc
     5101 attctctttg atgaaccaac atcagctctc gatccagaaa tggttggaga ggtcctcaaa
     5161 atcatgcaag atctggctca ggaaggcttg accatgattg tcgtaaccca tgaaatggaa
     5221 tttgcccgtg atgtctctca ccgtgttatc tttatggata agggtgtgat tgctgaagaa
     5281 ggtaaaccag aagacctctt cactaatcct aaagaagacc gtacaaaaga atttcttcaa
     5341 cgttatctca tactcaatga aaatcaaaga gcaaactagg cagctagccg caggttgctc
     5401 aaaacactgt tttgaggttg cagatagagc tgacgtggtt tgaagagatt ttcgaagagt
     5461 atcaaataaa aagaaaaggc tgcatcaacc gtgcagtctt tttgctgtcc tcaaaatgaa
     5521 aaggcagatt ctctatctct actacttgaa ggtcaagaat ccaccgttat ccaaagatta
     5581 atcttcaaat ttttcatgat ttttttcata gaaatcaatt aagcctaggg ctgtttcaaa
     5641 tgaaatattt tttacttttg cacgaccctg cgccagagca atgatagaca tttcacgcgc
     5701 attcgtttct ttagagatac ggtagcctgt gattttctta tcacgaaccc agccaacaac
     5761 tgattctact ttttcaaagt ttgacttagc catgtttttc tcctattttc ttctttataa
     5821 tatttaattg tatacgtttt tactactttt gtcaataaaa aaaacatata ttcaacgaaa
     5881 ataattagtt cagaattttc ttacttcctt tttaaatccg ataatatata ggttttgcta
     5941 gcctctacac cataaatatt tgctaataaa aatactgtat tattaccctc ttaaggtgca
     6001 aaattattca agttcttaaa aaatgcttct ctatttccgt aagatagctt atttcttcga
     6061 tttaagaact gaagatgata gttctaacct ttagaaatcc aagccttctc tagctcaaaa
     6121 actcctatct ttcttcctca ataaatcatc tgagagatag gtattcctta tatccttcaa
     6181 attcatcact cctttggata ttgaggtgct agcctagaag cagacacccg attatctaaa
     6241 ccatccttaa tagagatgac aagacagata agctcagtta attacttata tatcttttta
     6301 atatggtata tttgtcaaca aaaagaaatc aaatcatacc ttatactcaa tgaaaatcaa
     6361 agagcaaact aggaagctag ccgcaaattg ctcaaaacac tgttttgagg tttcagatag
     6421 agctaacgtg gtttgaagag attttcgaag agtattaaag acagtatgac tatgcttgtc
     6481 aatagatagg aaatcaaaat gaaggatgga aatctgagcc aaacatcata agtgctataa
     6541 tgaacagaag taagaattgc acgataagtt ttctaaaacc ataggaatcg tcccctttct
     6601 tcatcttcat tatagcacat atacatcagt tgtacaaata atatgatatt tttttattag
     6661 tcctcagaca agcatattat aaagcgtttc attaccattt aagttaatat aagctggatc
     6721 aaaaccttcc attctacgga tcaaacctgc ataatcatgc ttatctgcca aagcgatccc
     6781 aatcaatact gggcctgttc ccttgctagc tcgtttgata tactcaaaac gtgtgatatc
     6841 atcatttggc cccaggatat catttacaaa ctcacgcaaa gctcctggac gctgtgggaa
     6901 attgaccaca aagtaatgtt tgataccatc ataaatcaag gcacgctctt ccatttctgg
     6961 catacggttg atatcattat ttcctccaga aatgatacaa caaatggttt tccccttaat
     7021 atattcagct aaaacctcta aagaggcgat actagccgct ccagcaggtt ctgcgactat
     7081 cccttgctta gagtaaaggt caatcaaggt ttcagaaatc aatccctcat cgacacctac
     7141 caaagtttga atatgttgac gagttgcttc ataggtcaac tgacctacct tttgcacagc
     7201 aatcccatcc gcaaatttat caatttcctt gagtttgact ggacctccag cctcaaaggc
     7261 agctttcatg gaacgcgctc cattcgcctc tactccgata acctcaatct ctggacttgt
     7321 ttccttgata taggtagaaa ccccggcaat gagaccgcca ccaccaacag gaaccaagac
     7381 agcatcaaaa tcaatcgatt cttttcgagc ttcttctaaa atctcataag caactgttcc
     7441 ttgacctgct tgaacatgag catcatcaaa aggatcaata aaggtacgat tttcagagac
     7501 tgtaaattct tgagctgctt tggctgaggc atcaaaggta tctccaacta gtttaatagt
     7561 tacaaaatcc ccaccaaaaa agcgaacctg accaatcttt tgttgtggcg tagtaatagg
     7621 cataaagata gtagcaggaa ttttcatttc attacaagta taggctactc cctgcgcatg
     7681 atttcccgca gaagcgcaga ctaccccacg ttcacgttct tccttgctga gctgggaaat
     7741 ggcataatag gcaccacgaa ttttaaagga gcgaacacgc tgggcatttt cttttttcaa
     7801 ataaatctta gcaccatact tctccgataa ataatgatcg taatccagtg gagtattcac
     7861 aaccacaccg ttcaagacct tgtgagcctt gattatatct tttgaactta acatataaaa
     7921 cctctttttc tataaaaaac agtcactaat gactgttttt tagttattca tctccgaaaa
     7981 cttcagtaac actttcatgg tatttatgag ccatacgaat cgaaaactct ttaggaagtt
     8041 cataacgatt tgtggaaatc caatcatata accattctac caaactttct tcactatcat
     8101 tacgatggaa gataaaatag tttaaaattt ctttatattc tacatcattc aaatccaacg
     8161 tgtctacaaa ttgactacga ggaacaaaat tagaatctaa ttttcttagt tgatctgtca
     8221 attctcccaa ttgtaaatcc aactgaaaaa atagtttttg cagttgccta tcagtttcat
     8281 cacatcctgt cttgatagaa aacaaattgg taatattcat atgatttcaa cccctaaata
     8341 agtacatcta atatcccacg aatagctgaa gctaaagttt tcgcagtcca gtcatttaaa
     8401 cccgattctt tcaatccaga tgcaataatt tgctctattt gatataaacc gccatctatc
     8461 gtctcattta ttctgcgaag aggaccttca atataggata aagcttctcc gaatctaaca
     8521 gctgatttag gtcctaatga tttaccaact atatttgtta atgtttttta tttctcaaaa
     8581 tccatttcgc tatataacga gcagatttag tcgcaccagc tctctcaaac ggagaatgaa
     8641 tttcaagacc ttcttctcta gctatttgtg ccaatttgaa gaattgttca aaggtcatat
     8701 gcaacctacc atcttcaaaa ttagcatcat cataaatgtt aattacagca ttttcatcat
     8761 acgaccaatc tgctttattt tggtctgcta gcactattac cgacggtgta attacattat
     8821 acaactagaa ccaataataa cgatgcaatt tttctcataa tctttttcat aaaatcatct
     8881 ccagcgctat atatttctaa ttagttatag attttgaatg catcatcgtc gtttttacca
     8941 acaaatggca ttgctttacg caattctgca ccaacttttt caatttcaag gttagctgct
     9001 tgttcacggt aagcagtcaa ttttggacgt ccagctttat agtcatttac aaagtcattt
     9061 gcaaatttac cattttggat gtctgccaag acagccttca tattttcttt aacttgttca
     9121 gtgattacac gtggacctga tacatagtca ccgtattcag cagtgtttga aatagattga
     9181 cgcattttct tgaatccacc ttcgtagatc aagtcaacga tcaatttcat ttcgtgaaga
     9241 acttcaaagt aagccaattc tggagcgtaa cctgcttctg tcaagacttc gaaacctgct
     9301 tcgataaggg cagtcaaacc accacaaagt acagcttgtt caccaaacaa atcttcttca
     9361 gtttcttctt tgtaagttgt ttcaagaaga cctacacgag ccgctccaac acctttacac
     9421 cagtccatag caatgttttt agcatttcct gttgcatctt ggtatactgc ataaagagct
     9481 ggaacaccaa atccttcttc gtaagtacga cgtaccaagt gtcccggtcc tttaggagca
     9541 cacatgaaga catctacatc cgcaggaact ttgataaatt caaagtggat gttgaaacca
     9601 tgggcaaatc caactgcgtt tccagcttcc aagtttggag cgatttctgc ttcgtacaat
     9661 tcttgttgaa tttcgtctgg cgccaagatc atgataacat cagccaactt agtagcttct
     9721 actactgtgt aagtatcaaa tccatcttct tttgctttat caaaagattt acctggacgt
     9781 acaccgataa taacgtcacg acctgaatca cgcaagtttt gagcatgcgc atgtccttgt
     9841 gaaccataac cgataacggc gattttttta ccgtcaagtg ctgctacttt aacatctttt
     9901 tcatattcca tttgaactgt catagctttt ctctcttttc tattatttat tgccttttag
     9961 gctggtttaa taaatttaag ttggattttt aatcgcgggt aaatccagtt gcacccgttc
    10021 gagcaatatt gcgaataccg tatgggcgaa tgactcgcaa tagggcttcg ctcttttctg
    10081 catttcccgt catctgaatg gtaatcgagc ttggcgctac gtctaccact gttgcacgga
    10141 aaggttgaat aatcgctaaa atctcagctc tcttctcagc tggcgctgac atcttaacca
    10201 aaatcacctc gcgctccaaa tgaggcttgt ctgtaatatc tcgaatgcga atcacatcaa
    10261 tctgacgatt gagctgtttg atgatttgct ccacttcatc atgagaagca acatcaataa
    10321 taatagtgat acgcgataca ttcggatctt ctgttgctcc aacagagatg ctttcaatat
    10381 taacctgacg acgagatagg acacctgtaa agcgattgag gactcctgaa cgattttgta
    10441 gttttgctgt taacattcta cgcatggaac tgcaccccca acatctcatg attactctta
    10501 ccagccggta ccattggtaa cacctgttcc ttacgagaaa tatctacctc aattagcata
    10561 ggaacatcct cagtgatgac ttcaaggtct tgagccaagg tctcaggatt gtcaaactta
    10621 tagtttttaa taccataagc ctgcgccatc aattggaaat caggaagggt atcaaagacc
    10681 gactctgatg ttctgccttc atagaaggat tcctgccact ggcgaaccat tccaagtgaa
    10741 tgattgttca gcataaccac cttgattggc accttgtaaa tattcaaaat agccaactcc
    10801 tggttggtca tttggaaacc accatcccca acaaacaaga ctacttcctt atctgggtta
    10861 gcaattttag caccgattgc tgctggaatt ccaaagccca ttgttcccaa accacctgaa
    10921 gtcactaact gacgctcatt ttggtaggga taatactgag ctgtccacat ttggtgttga
    10981 ccaacgtctg ttaccacaat ggcatctcca ttcgtcaatt caccaattcg ttcaataact
    11041 gcttgcggtt gaaccacacg ctctttcttg tcataagaac gaacacgatt cttgtcttta
    11101 gtgactttct caatccactt ttcagtattg ttgtgaactg ttggttctgc tagcaacatt
    11161 tgcaaggcct tcttagcatc tccaactaca ggaatgtctg cactgataat cttgccaatc
    11221 tcagctgggt caatatcaat gtgggcaacc ttagcattct tagcgaaagt cttaggattc
    11281 cccgtcaaac ggtcatcgaa acgagaacca atactaatca taaagtccgc ttccgtcatg
    11341 gcaatatttg ctgcgaatga cccgtgcatg cctcccattc caagaaagag tgggtgactc
    11401 gttgcaatcg ttccttgtcc caaaagactg gttaccactg gaatttgata gcgttctgca
    11461 aattcattta gttccgtagc agcctcagca taactaattc caccaccagc taacaagact
    11521 ggctttttag ccttggacaa ttgcttcaag attttcttga tttgcatatc attcggctca
    11581 agagtcggct gataacttgg taaattcact tctggtgaat aaatgaagtc tgtttctaaa
    11641 gcagatatgt cttttggtag gtcaattaca actggccctg gacggcctgt agttgcgata
    11701 tggacagctt ccgtaatgat acgcggaata tcagctgtct cacgaacttg gtaattgtac
    11761 ttagtgattg gcatggtaat tcctacgatg tctgcctcct gaaaggcatc cttcccaatc
    11821 cctgctcgcg ccacctgacc tgtaaagacc aaaaggggaa cgctatcgct catagcatcc
    11881 gcaatccctg taatggcatt tgttgctcct ggtccactag tgacgacggc aacacccaac
    11941 tttccagttg atttggcata accttcagct tcatgcaaac aaccttgctc atggcgccct
    12001 agaatgtggc gaatgccttt aaaattatat atcgcatcat aaaaaggcaa aaccgcacca
    12061 ccaggataac caaagatggt atcaactcct aaatcacgaa gtgtttccaa aactaggtcc
    12121 gaccccgtct taggagattc taaactgatt ttctccattg ttcccctttc tttcctctta
    12181 aaaataactt gttactatca taccattttt tcaaaatttt tcaagataaa agaagaaatt
    12241 ttctgaattt tctattttaa cgtttattta tgaatgttat ttttgttttt attaaaaaga
    12301 taccgatttc atttactaaa aaagaaaaaa gaactgattt ctcagtcctt cattaatctt
    12361 attccacact aaataggtat gggtaaacag gttgttgacc ttggtgaatc tcgacttcaa
    12421 cgtcttcgaa ttcttctacg atttcttgag cgatttcatt ggcaagttct tcgcttccgt
    12481 cttcaccgac atagaaggtt acgatttcac tatcttcatc caacatatgt ttcaaggttt
    12541 cagtcaatgt ttggtgcata tcagggtttg acacaagaat ttttccatcc accataccta
    12601 gattatcgtt ttcatggatt tctaagccat cgatcgttgt atcacgcacg gctgttgtga
    12661 cgcttccgct aacgacatcg ctaagagcag ctgtcatacg ctcttggttt tcttcaatgg
    12721 acttgcttgg atcaaaggca agaagacttg tcataccttg aggaagagtg cgagcctcta
    12781 ccactactgc tggttgctcc aaaacttctg ccgcagattg agctgccatg aagatgttct
    12841 tgttgtttgg caagaagatg atgttacggg cattgacctg ttcaacagcc ttgataaagt
    12901 cttctgttga agggttcatg gtttgaccgc cttcgataac ataatccacg ccttgagaac
    12961 agaagatatc tgctagacct ttaccagcca ccacagcaat caaagcatac tctttttctt
    13021 cagccgactt gataacttga gtagcttctt tctcaacctg tgcttcgtgt tggttacgca
    13081 tattgtcaac ttttaccttg accaagctac catatttgag accttcttgc ataacaagtc
    13141 ctggatcttc tgtatgaaca tggactttga caatttcatc atcgttaaca acgaggagag
    13201 aatccccaag ctcatccaag tagttacgga attcatcgta gtcaaaatct ttagcatagg
    13261 ttggaccttg cttaagagct accatgattt cagtacagta accaaacgtg atgtcctcag
    13321 tcgctacgtg accagctaca gacttatgat gctctacatt gatcatctca ctcatgttgg
    13381 caggagtcgc tacaaagtcc tcagatgcaa tatattcgcc agtaagggct gaaaggaaac
    13441 cttcgtagat gaagaccaat ccttgaccac ctgagtccac aacgccaact tctttcaata
    13501 ctggaagcat gtctggtgtt ttagctagag ctgttttagc accttccaag gctgcgcgca
    13561 tgacttcaac agtgtcatct gtttgctcag cttttttctt agcaccgata gcagctccac
    13621 gagaaactgt caaaatcgtt ccttcaacag gtttcatcac tgccttatag gcaacttcca
    13681 cacctgattg gaaggccaga gccaagtctt gacctgttaa ctcgtcttta tccttgatag
    13741 cttgggaaaa tccacggaaa agctgagacg taatcactcc tgagttccca cgcgcaccca
    13801 tcaaaagccc tttggcaaga atgctcgcta cttctccaac tgtagaagct ggcttgtctg
    13861 caacttcttt agcaccattt tcaatggtca ttcccatatt tgtcccagta tctccatctg
    13921 gaactggaaa gacgtttaat gaattgacat attcagcttg cttattcaag cgagttgatg
    13981 cagcctgcac catttcttga aataagctag tagtaatttt tgacacggtt attctcctac
    14041 aactttgata ttttgaatgt agacatttac agtctgagca gtaattccaa gctggttttc
    14101 caagctaaag gcaacacgct cttgaatgtt ttttgacact tcactaatct ttgttccgta
    14161 gcttaacacg gtatatacat caactgcaat actgccatct tcggctgcct ttacgacgac
    14221 acctttagaa taattttcct tacctagcag ggcttggaaa ttatctttga gggcattttt
    14281 actagccata ccgaccacac caaaaatctc agttgctgct ccacctacga cggttgcaat
    14341 cacttcatct gttagttcga tttgaccatc ttttgtatta atttttacag tcatcctttt
    14401 tacctcaact agttgatact ctattttatc atatttcagc ccaagtgtaa aagcagaatg
    14461 atgtatcagc ggatatttac tctatttttc aaacgatgtt atacccacaa taaaagaaaa
    14521 aagaccctaa ggtctccttt gcttttatta ttaaacgcgt tcaactttac ctgatttcaa
    14581 agcacgagct gaagcccaaa cttttttagg tttaccatcg ataagaacag taactttttg
    14641 aaggtttggt tttacggcac gttttgtttg gttcatcgcg tgtgaacggt tgtttcctga
    14701 tacagtctta cgacctgtaa agtaacatac tttagccatt gtgttttcct cctattagat
    14761 ctaatatagc ggatgtgcta gcaccacata ccgtactatg ttatcacact ttcttgtttt
    14821 ttgcaaggga attggaagat tttttatttg tgtcttaaat caggtcttgc gtgacatttt
    14881 ctgctctcca catgccatcg ttgattaaca gaacaccaga attaaaatta tgtgtataaa
    14941 aatcatctct aactgcagct aagggtatag ccgtcaagtc caaatcccac agctcatcta
    15001 tcgattttct tacaacaata tctgaatcca aatacagtac acgagactcg cttacatact
    15061 ttggaataaa atacctaaaa aagccgcata tgaaagtccc tcaaagggga gacgataacc
    15121 tttcagaata ttactgtcaa tctaaacatt cacaatctca ctattcaaag tctctagtct
    15181 tttttccatc aattggaacc attctcgcgg aaggtcatca ttaaaaacat aaaacttaag
    15241 attataatga tgaacacaaa gagattttat tgttgtttca actttatcca tataagcatt
    15301 atctgcacct aagacaatcg cttttttctc ttttttctct tctttcactt tttatctcat
    15361 ttctttttat tcccatcata tgtttctacg taaccattat tttcgcctat tcgttcgtaa
    15421 aaccatacca gtggagattt tagatgaagt cccattacgg tttacaattt ttacattacg
    15481 acacggagtt ttacaaatcg atttcatttg ccaaacgtag ttagtgaggc agttagctag
    15541 ttcgccaaat agcgactagc gtccaacaat ttggaacttt agttccaatt gttggtactg
    15601 agtcacatct tctcctctaa ctctacgtct ggatacttgt cagcaaacca gcggagggca
    15661 aagtcatttt caaagagaaa gactggttgg tcaaaacggt ctttggctaa gatattgcga
    15721 cttgacgaca tccgttcatc caagtcctca ggcttgatcc aacgaacggt ctttttaccc
    15781 attgggttca taactacttc tgcattgtac tcgccttcca tgcggtgttt aaagacttca
    15841 aactggagtt gacctacagc gcctagcatg tactcacctg tttggtaatt cttataaagc
    15901 tgaacggctc cttcttgcac caattgctca atccccttgt ggaaggattt ttgcttcata
    15961 acattcttag cagaaacttt catgaaaatt tcaggagtaa aggttggcag tggttcaaat
    16021 tcaaacttgt ttttaccaac tgtcaaggta tccccaactt gataagtacc ggtatcgtaa
    16081 accccgataa tatcacctgc cacggcattg atcacattct cacgactttc cgccataaac
    16141 tgggtaacat tagatagttt ggccccctta ccagtacgag ggagattgac actcatgcca
    16201 cgctcaaatt cgcctgatac gatacggaca aaggcaatac ggtcacggtg acgaggatcc
    16261 atgttggctt ggattttaaa gacaaagcct gagaaatcct tgtcataagg atccacaatt
    16321 tcaccgtctg ttttcttgtg accatgtggt tctggagcaa acttgaggaa gatttcaagg
    16381 aaggtctgca caccaaagtt tgtcagggct gaaccgaaaa agacaggcgt caattctcca
    16441 gccagaatag cttcctctga aaactcattc ccagcttcat ttaaaagctc aatgtcatcc
    16501 ttgacttgct cgtagaaagg attgctacca aagagtttgt ccccgtcttc tagactggca
    16561 aaacgctcat cccctttgta aagctctaaa cgttggttat agaggtcata caagccctca
    16621 aaggctttcc ccatcccgat aggccagttc atagggtagc tagcaatgcc caagatttct
    16681 tccaattctt gcaagagatc caaaggctca cgaccgtcac ggtccagctt gttcataaag
    16741 gtaaaaactg gaatgccacg atgtttcaca acctcaaaca atttcttggt ttgagcctcg
    16801 atccccttgg cagagtccac gaccatgacc gcagcatcca ccgccatcaa ggtacgatag
    16861 gtatcttctg agaagtcctc gtgccctggc gtgtctaaga tattcacgcg cttgccgtcg
    16921 tagtcaaatt gcataacaga tgaagtaaca gaaatcccac gttgcttctc gatatccatc
    16981 cagtcagatt tagcaaaagt ccctgttttc ttccctttta ccgtaccagc ctcacgaatc
    17041 tcacccccaa agtagagtaa ctgctcagtg atggttgttt tccccgcgtc cgggtgggag
    17101 ataatggcaa aggtacgacg tttcttaatt tcttcttgaa tattcataag ttctctttct
    17161 ttgattctct atttttcttg tttcaatagc tgagaatgat ttttacattg gattttacca
    17221 ttcctttcaa cattccatta tatcggattt tagcattttt ttcaatttct atttcttttc
    17281 acttccccct cccttattta taggaaaata tggtaaaata gaacagacta aaaatcatca
    17341 tttcacgaaa ggatgcaaga tgaaaattac gcaagaagag gtaacacacg ttgccaatct
    17401 ttcaaaatta agattctctg aagaagaaac tgctgccttt gcgaccacct tgtctaagat
    17461 tgttgacatg gttgaattgc tgggcgaagt tgacacaact ggtgtcgcac ctactacgac
    17521 tatggctgac cgcaagactg tactccgccc tgatgtggcc gaagaaggaa cagaccgtga
    17581 tcgcttgttt aaaaacgtac ctgaacaaga caactactat atcaaggtgc cagctatcct
    17641 agacgatgga ggagatgcct aatgactttt aacaataaaa ctattgaaga gttgcacaat
    17701 ctccttgtct ctaaggaaat ttctgcaaca gaattgaccc aagcaacact tgaaaatatc
    17761 aagtctcgtg aggaagccct caattcattt gtcaccatcg ctgaggagca agctcttgtt
    17821 caagctaaag ccattgatga agctggaatt gatgctaaca atgtcctttc aggaattcca
    17881 cttgctgtta aggataacat ctctacagac ggtattctca caactgctgc ctcaaaaatg
    17941 ctctacaact atgagccaat ctttgatgcg acagctgttg ccaatgcaaa aaccaaaggc
    18001 atgattgtcg ttggaaagac caacatggac gaatttgcta tgggtggttc cggtgaaact
    18061 tcacactacg gagcaactaa aaacgcttgg gaccacagca aggttcctgg tgggtcatca
    18121 agtggttctg ccgcagctgt agcctcagga caagttcgct tgtcacttgg ttctgatact
    18181 ggtggttcca tccgccaacc tgctgccttc aacggaatcg ttggtctcaa accaacctac
    18241 ggaacagttt cacgtttcgg tctcattgcc tttggtagct cattagacca gattggacct
    18301 tttgctccta ctgttaagga aaatgccctc ttgctcaacg ctattgccag cgaagatact
    18361 aaagactcta cttctgctcc tgtccgcatc gccgacttta cttcaaaaat cggccaagac
    18421 attaagggca tgaaaattgc tttgcctaag gaatacctag gcgaaggaat tgatccagag
    18481 gttaaggaaa caatcttaaa cgcggccaaa cactttgaaa aattgggtgc tatcgtcgaa
    18541 gaagtcagcc ttcctcactc taaatacggt gttgccgttt attacatcat cgcttcatca
    18601 gaagcttcat caaacttgca acgttttgac ggtatccgtt acggctaccg cgcagaagat
    18661 gcgactaatc ttgatgaaat ctatgtaaac agccgtagcc aaggttttgg tgaagaagtg
    18721 aagcgccgta tcatgctggg tactttcagt ctttcatcag gttactacga tgcctactac
    18781 aagaaggctg gacaggtccg tacacttatc attcaagatt tcgaaaaagt cttcgcggat
    18841 tacgatttga ttttgggtcc aactgctcca agtgttgcct atgacttgga ttctctcaac
    18901 catgacccag ttgccatgta cttagccgac ctcttgacca tacctgtaaa cttggcagga
    18961 ctgcctggaa tttcgattcc tgctggattc tctcaaggtc tacctgtcgg actccaattg
    19021 attggtccca agtactctga ggaaaccatt taccaagctg ctgctgcttt tgaagcaaca
    19081 acagactacc acaaacaaca acccgtgatt tttggaggtg acaactaatg aactttgaaa
    19141 cagtcatcgg acttgaagtc cacgtagagc tcaacaccaa ttcaaaaatc ttctcaccta
    19201 cttctgccca ctttggaaat gaccaaaatg ccaacactaa cgtgattgac tggtctttcc
    19261 caggagttct accagttctc aataaagggg ttgttgatgc cggtatcaag gctgctcttg
    19321 ccctcaacat ggacctccac aaaaaaatgc actttgaccg caagaactac ttctaccctg
    19381 ataatcccaa agcctaccaa atttctcagt ttgatgagcc aatcggttat aacggctgga
    19441 ttgaagtcga gctagaagac ggtacgacca agaaaattgg tatcgaacgc gcccacttag
    19501 aggaagacgc tggtaaaaac acccacggta cagacggcta ctcttacgtt gaccttaacc
    19561 gccaaggggt acctttgatt gagattgtat ctgaagccga tatgcgttcg ccagaggagg
    19621 cctatgctta tctgacagcc ctcaaggaag ttatccagta cgctggcatt tctgacgtta
    19681 agatggaaga aggttctatg cgtgtggatg ccaacatctc ccttcgtcct tatggtcaag
    19741 agaaattcgg taccaagact gaattgaaga acctcaactc cttctcaaac gttcgtaaag
    19801 gtcttgaata cgaagtccaa cgccaggctg aaattcttcg ctcaggtggt caaatccgcc
    19861 aagaaacacg ccgttacgat gaagcgaata aagcaaccat cctcatgcgt gtcaaggaag
    19921 gggctgctga ctaccgctac ttcccagaac cagacctacc cctctttgaa atttctgacg
    19981 agtggattga ggaaatgcgg actgagttgc cagagtttcc aaaagaacgt cgtgcgcgtt
    20041 atgtatctga ccttggttta tcagactacg atgctagtca gttgactgct aataaagtca
    20101 cttctgactt ctttgaaaaa gctgttgccc taggtggtga tgccaaacaa gtctctaact
    20161 ggctccaagg ggaagtcgct cagttcttga atgctgaagg taaaacactg gaacaaatcg
    20221 aattgacacc agaaaacttg gttgaaatga ttgccatcat cgaagacggt actatttcat
    20281 ctaagattgc caagaaagtc tttgtccatc tagctaaaaa tggcggtggc gcgcgtgaat
    20341 acgtggaaaa agcaggtatg gttcaaattt cagatccagc tatcttgatc ccaatcatcc
    20401 accaagtctt tgccgataac gaagctgctg ttgccgactt caagtcaggc aaacgtaacg
    20461 ccgacaaagc ctttacagga ttccttatga aggcaaccaa aggccaagcc aacccacaag
    20521 ttgcccttaa actacttgca caggaattgg cgaagttgaa agaaaactag acagaacaaa
    20581 accagcccta aggttggttt tttcttctct accaactccc aataactatt ttggctttat
    20641 ttccagagta ttttatggta aaatgaagag taataatatt tattaaagag gtaaaaacat
    20701 gattgaagca agtaaattaa aagctggtat gacctttgaa acagctgacg gcaaattgat
    20761 tcgcgttttg gaagctagtc accacaagcc aggtaaagga aacacgatca tgcgtatgaa
    20821 attgcgtgat gtccgtactg gttctacatt tgacacaagc taccgtccag aggaaaaatt
    20881 tgaacaagct attatcgaga ctgtcccagc tcaatacttg tacaaaatgg atgacacagc
    20941 atacttcatg aatacagaaa cttacgacca gtacgaaatc cctgtagtca atgttgaaaa
    21001 cgaattgctt tacatccttg aaaactctga tgtgaaaatc caattctacg gaactgaagt
    21061 gatcggtgtc accgttccta ctactgttga gttgacagtt gctgaaactc aaccatctat
    21121 caaaggtgct actgttacag gttctggtaa accagcaacg atggaaactg gacttgtcgt
    21181 aaacgttcca gacttcatcg aagcaggaca aaaactcgtt atcaacactg cagaaggaac
    21241 ttacgtttct cgtgcctaat ctctagaaag aggtcattct atgggaattg aagaacaact
    21301 tggcgaaatc gttatcgccc cacgtgtact tgaaaaaatc attgctatcg ctactgcaaa
    21361 ggtagagggt gttcactctt tttcaaacag atcagtgtct gatacccttt caaaactttc
    21421 actcggccgt ggcgtttatc ttaaaaacgt ggacgaagaa ctcacagcag atatctatct
    21481 ctaccttgag tacggagtaa aagttcctaa ggtagcggtt gctatccaga aagctgtcaa
    21541 agatgccgtc cgtaatatgg ctgatgtaga actcgctgct atcaatattc acgttgcagg
    21601 tatcgtccca gataaaacac caaaaccaga attgaaagat ctatttgacg aggacttcct
    21661 caatgactag tccactatta gaatctagac gccaactccg taaatgcgct tttcaagctc
    21721 tcatgagcct tgagttcggt acgaatgtcg aaactgcttg tcgtttcgcc tatactcatg
    21781 atcgtgaaga tacggatgta caacttccag cctttttgat agacctcgtt tctggtgttc
    21841 aagctaaaaa ggaagaacta gataagcaaa tcactcagca tttaaaagta ggttggacca
    21901 ttgaacgctt aacgctcgtg gagagaaacc tccttcgctt gggagttttt gaaatcactt
    21961 catttgacac tcctcagctg gttgctgtta atgaagctat cgagcttgca aaggacttct
    22021 ccgatcaaaa atctgcccgt tttatcaatg gactgctcag ccagtttgta acagaagaac
    22081 aataaaaaga aaaagtgttt gatagatatc aaacactttt tctttgcctc ccactatcta
    22141 aaaaaatgat aatagatata attgtaaaca aaaatccaga taggttttgc atgattgaga
    22201 aagttaaaaa aactatggca gagaatcgtt aatctcagat tgtcggtaga acgataaaca
    22261 agggcaaaaa agaaaccaat cagactataa tataataaac taattggatc tctgtgagat
    22321 agtatcaaat ggctaatccc aaagataagg cctgataaga tgacatctaa atagtacttt
    22381 gacttaggaa agaaggtatt cataaaatac cctctatcaa gagtctcctc aaaaacagga
    22441 ccgatgatta caagcaggac aaaagataag atagtcgata aaaaggttgg ttgtccattt
    22501 gaaagaacca cggtaaaata ctcatcatga atattcctat ggttaatcaa atgagcatag
    22561 cgtgcctaaa aattaccgag aatctgataa accacataag ttgcaaataa ggagaagaca
    22621 aatgaccagt cccagctctt tttctttttt aacctccaaa ttaatagaaa aggaaacttc
    22681 ccactaatcc cactgttaaa ataagagaat agacatcagc tcctaaccct aaaatgatcg
    22741 tcacatacaa tccaattgtt tgtggtaaat aggtagatag taaaataata agcaaaaata
    22801 ttccaaattg tcttagtttc tttgtgtttc tcatcgtact tttttgaaag attaccctgt
    22861 tcggaagccg tacttccaag catctatata agaattaagt gccccttgcc tcatataggg
    22921 agcaaattct ctataatata accatctact atatccatct tcccaaacag caagaccacc
    22981 tgaagtttgc tccaagtcct cagttgaaag aactgtaaat gtttctgtac ctgtcattgc
    23041 aagtaccttc ttaaaataga ttgttgtagg ctcacattta tagtatattt ctttttttgt
    23101 ctattttata gtctatctcc tcaactggca atttttcgac ctgaattgca tttttccata
    23161 aaaaatgaga cctttctagt ctcatttagt cattcttagt attttctaaa tcgttgatag
    23221 cgttcttcca gcaactcttc tagcggtttt tgtgaaagtc tagccagctc cgtttggagt
    23281 tcttttttga cactcttaat tagttcttta ctagaaagtc ctacttcaga aatcacctta
    23341 tccaccacgt ccatttctaa cagttcatgc gaagtgattt tcatcagttc tgctgcttcc
    23401 atggcgcgag taccgtcctt ccataaaatg gaagcaaagc cttctggact gagaatggca
    23461 tagatagaat tttccagcat ccagacacgg tccgcgacag ctagagccag agccccgcct
    23521 gaaccacctt caccgataat aatggcgata ataggaactt tcaggtcact catttccatg
    23581 agattgcgag cgatagcttc cccttgacca cgttcttccg ctccgacacc aggataagca
    23641 cctgctgtat tgataaaggt cacaactgga cggccaaatt tctcagcctg tttcatcaac
    23701 cgcagtgcct ttcggtagcc ttctggatgt ggttggccaa aattccgttt gaggttgtct
    23761 tgcaaactct tgcctttttg gataccaacc actgttacag cttggtctcc aagccaacca
    23821 ataccaccaa caactgcacc atcatcacga aaagaacggt caccatgtaa ttggataaat
    23881 tcatcaaaaa tgcctgtcgc aaagtccaag gttgtcaagc gactctgctc acgcgcttct
    23941 ctgactattt ttgcaatatt catctaggac tccctccatg caatctgact aggctagcaa
    24001 tcgtatctgg taagtctctt cttttgacaa tagcatccac aaagccatgt tctaatagga
    24061 attctgcctt ttggaaatcc tcaggcaagc tttcacgaac cgtattttca atcacacgac
    24121 gcccagcaaa accaaccaag ctctgtggtt cagccagaat gatatcgcct tccatagcga
    24181 aagaagctgt cacaccacca gtcgttggat ctgtcaaaat ggtcaggtaa aagagaccag
    24241 catttgaatg gcgtttaacc gctgcagaga tcttagccat ctgcatgaga ctcatgattc
    24301 cttcctgcat acgggctcca ccagaggctg tgaataggac aactggtaat ttttcgacag
    24361 tcgcatactc aaacaaacga gtgatttttt cacctacaac cgtacccata gaagccatga
    24421 taaaattaga atccataatc ccaagagcca cagtctgacc tttaataaga gcagttcctg
    24481 tcacaacggc ttcatgcaga cctgtttttt cacgcataga tgccagtttc ttttggtaac
    24541 cagggaaatg caagggatcc ttgctttcaa tccctgtaaa caattctttg aaggttccca
    24601 tatcaatcgt caaagccaag cgttcttggg cagaaatacg aaaggtatag ctacagtgcg
    24661 gacagatacg ttcacttccc agatccttct gatagatggt atgcttacag cctggacact
    24721 gggaaaataa ttcatctgga acctctggct tagcttgagg tttttcccta accgaacgat
    24781 tgggattgat tcgaatatac ttatcttttt tactaaatag agccattgat tccccttttc
    24841 ggtttaaact cttaaagtca ttttattctt tttcttgata tttaggtaag aaggtttcca
    24901 tcaagaagga agtatcataa tccccagcaa tgacattgcg atctgaaatg agatcaagct
    24961 ggaaatctgc attggtctgc actccttcaa tctctaattc atagagggca cgttgcattt
    25021 tcatcaaggc gtcaaaacga ttttcgccgt gtactatgat tttggcaatc atactatcat
    25081 aataaggcgg aatggtataa cctggataaa ctgctgaatc cacgcgcaag ccaactccac
    25141 cacttggcag atagagatta gtaatcttac ctggacttgg agcaaagtta aaggctgggt
    25201 tttctgcatt gatacgacac tcgatggcat gaccgcgtag gacaatatct tcttgcttaa
    25261 cagacaaagg ctgacctgcc gcaatgcaaa tctgttcctt aacgatatca acacctgaaa
    25321 caaactctgt tactggatgt tctacctgaa cacgagtatt catctccatg aaatagaaat
    25381 tgctacttgc ttcatcaaga agaaattcaa tggttcctgc attctcatag ccaacaaact
    25441 ctgccgctcg aacagcagca gcacctattt catgacgcag cgtttttccg attgcaatcg
    25501 agggactttc ttccaaaacc ttttggttat tcctttgaag agaacaatcc cgttcaccca
    25561 agtgaatcac atgtccatgc tcatcaccta ggatttgaac ctcaatgtgc cgagctggat
    25621 agataacccg ttctatgtac atggcaccat tgccataatt ggccttggcc tcactagagg
    25681 cagtttcaaa ggcagaaacg aggtcatctg gtttttcaac cttacgaatc cctttaccac
    25741 ctccacctgc tgaagccttg agcataacag gatagccaat tttttcagca acaatcaaag
    25801 cttcttcaga gttatgcact tctccatctg aacctggtat aacaggcaca cctgctttaa
    25861 tcatctgagc acgcgcattg atcttatccc ccatcatatc cataacatga ccagatggac
    25921 cgataaactt gatacctact tcttcacaca tggtcgcaaa tttggaattt tcactgagaa
    25981 atccaaaacc agggtgaata gcttctgcct cagtcaagac tgcagctgat agaactgcat
    26041 taatattgag ataagactct gttgccttgc caggaccaat acaaactgct tcatctgcca
    26101 acagcgtatg aagagcttcc ttatcagcag ttgaataaac cgctaccgtc gcaatcccca
    26161 attcacgtgc cgcacggata atacgaaccg caatttcacc acgattggca attaaaattt
    26221 ttcgaaacat ggagaacctc cttagttccc aattgcaaaa gtaagggtac cactggctgc
    26281 aagcttgcca tccacttcag cctttgcttc aaccacagct atggtgccac gacgttttac
    26341 aaaagtcgct gtcataacca attggtcgcc tggtacaact tgcttcttga acttaacctt
    26401 atccatacca gcgtaaaaga ccagttttcc tttattttca ggttttgata actccaacac
    26461 accagcagtt tgcgccaagg cttccataat cagaacacct ggcataactg ggtattgagg
    26521 aaagtggccg ttaaagaaag gctcgttgat ggtcacattt ttgatagcaa caatggtatc
    26581 ctcgctcacc tccaagacac ggtccactag aagcatagga taacggtggg gaagggcttc
    26641 tttgattcct tgaatatcga tcatttgata cgtaccaatc ctttaccaaa ctcaaccatt
    26701 tcttcgttag agacgagaat ttccgttacc acaccatcct taggagctgg gatttcattc
    26761 atgactttca tggcttcgat aattaccaat gtttgacctt ttttgacact atcaccaact
    26821 gtaacgaagg caggtttatc tggtccagca gccaagtaaa ccactccaac aagtggactc
    26881 tctacaagat ttccctcact agccacactt gcttcagctg gagctggaac ttcttctgct
    26941 acagtctctg ctggagcaga tgtaggagct actggactcg gtgttgctag aacgggtgct
    27001 ggagcgactt gagttgcaac ttcaggcaca ggtctcgctt cattcttgct aaactgcaac
    27061 tcatccgtcc catttttata agaaaattct ctcaaacttg actggtcaaa ttgagtcatc
    27121 aagtctttaa tatcgtttaa attcatactt atctattctc ccaacgtttg aaagcaagaa
    27181 ctgcattgtg gcctccaaaa ccaaaagtat ttgaaatagc gtatggaatt tctttctcca
    27241 agccttgtcc ataaacgaca ttagcttcga tataatctga tacttcactt gtcccagctg
    27301 tcattggtac aaagttatga cgcatagctt cgatggtgac gatagcttct actgcacccg
    27361 cagcccccag caaatgtcct gtaaaagact tggttgatga tacaggtact tccttaccaa
    27421 gaacagctac gatagcacca ctttctcctt tttcattggc aggagttgac gttccgtgag
    27481 cattgacata ggctacttgc tctggagaaa tctcagcttc ttccaaggct agtttgatgg
    27541 ccttgatagc tccctgacct tctggatgtg gagaagtcat gtggtaggca tcacaagtat
    27601 ttccgtaacc aaccacttca gccaggatag tagctccacg tttttcagcg tgttcaagac
    27661 tttctagaac caacatccct gaaccttcac ccataacaaa cccattgcga tccttatcaa
    27721 atgggatcga agcacgagtt ggatcctctg tagtagagag agctgttaag gcttggaaac
    27781 cagcgatggc aaaaggtgtg atagaagctt ctgttcctcc caccaacatc acatcttgga
    27841 aaccaaactt aatggagcgg aaggcatccc caatcgcatc atttgatgaa gagcaggcag
    27901 tattgataga tttacaaaca ccgtttgcac caaaacgcat ggctacattc ccagaagcca
    27961 tatttggtaa agcttttgga agagtcattg gtttgacacg tttgggtcct ttttcatgaa
    28021 ggcgaagtac ctgatcttca atttccttga ttccaccaat accagatgca acgataacac
    28081 caaaacgatc cctattaaga gcctctacat caagattggc atgatttaca gcctcttggg
    28141 ctgcatacaa ggcatataaa gaatagttat caaaacggtt ggtatctttt tttacaaagt
    28201 atttatcgaa cggaaaatct tggatttctg ccgcattatg cacatcaaag tcactatgat
    28261 caaattttgt aatgccacca atgccgattt tcccagttgc taaactattc caaaattctt
    28321 ctggtgtatt tccgattgga gatgttactc cataacctgt taccactact cgatttagtt
    28381 tcattctttt cacctctagc tttcgctaca tacttaagcc accatcaatg gcaatcactt
    28441 gaccagttag ataatcttgg cctgctaaaa atactgtcaa atctgcaacc tgctctgcct
    28501 gcccaaattc tttcatcgga atctgagcta gtgtagcttc cttaatctta tctgataaga
    28561 tagctgtcat atcagactca atcattcctg gagcaatcac attgactcgt atattccgac
    28621 tagcgacctc gcgtgccaca gacttggtaa agccaatcaa gccagcctta gaagcagcat
    28681 agttagcttg accaatattc cccatcaaac caacaacact agacatatta atgatagcac
    28741 cttctctggc tttcatcatc ggtttcaaga ctgattgtgt catattaaag gcaccagtca
    28801 gattgacctt gagcactttt tcaaaatctg cttctgtcat cttgagcata agagtatctt
    28861 gggtaatccc tgcattgttg accaaaacat ctactgaacc cagttctgca atagcttgat
    28921 caatcatacg cttagcgtct gcaaaatctg atacatctcc tgaaatggga accaccttga
    28981 taccatagtt tgaaaactca gcgagcaatt cttctgagat tgccccacga ctgtttaaga
    29041 caatgttggc tcctgcttga gcaaacttgt gggcgatggc aagaccaatt ccacgactcg
    29101 aacctgtaat aaagatattt ttatgttcta gtttcatttt tttcctttca aaacttctac
    29161 ttattttagt ctatttttct aaaagtgcta ctaaactcgc ttgatcttcc acatgagcta
    29221 agtgagcagt ttgatcaatt tttttaacaa aacctgacaa gactttcccc ggtccaatct
    29281 cgataaagtt gcttatgcct gcttcttgca tgaccccaat actttcatag aaacgaacgg
    29341 gttccttgac ctgacgcgtc aagagctgag caatgtcctc tttttgcatc acagcagctt
    29401 ctgtattgcc gactagggga caagtaaaat ctgaaaaact tacctgagct agagtttcag
    29461 ctagtttctg gctagcaggc tcaaggagag cggtgtgaaa gggacctgac accttaagag
    29521 gaatcaagcg tttggcacct gcttcttgca aaagttcaac cgctcgatca actgcaacca
    29581 cttctccagc aatgacgatt tgtgcaggtg tgttatagtt ggctggagta accactccaa
    29641 gttcagaagc tttttgacag gcttcttcaa tgacctctac tggcgtattg agaactgcta
    29701 ccatcttgcc agagtcagca ggagccgctt cttccatata ggctccacgc ttagctacca
    29761 aggcaaccgc atcttcaaaa tccaaggcgc cgcttgccac caaggcagag tattctccaa
    29821 gagacaaacc agcaaccata tcaggctgat agcccttttc ttgcaataaa cggtagatag
    29881 caaccgaagt cgctagaatg gctggttgcg tatagcgggt ctgattgagt ttgtcttctt
    29941 ccgtatcgat gagataacgt aaatcataac cgagcacctg actcgctcga tcaatcgttt
    30001 ctttaacaat cggatactga tcatagaaat cccgtcccat ccctagatac tgggcacctt
    30061 gaccagcaaa taaaaaggct gttttagtca tttcttacaa ctcctgccca gcgagaggct
    30121 tcttcttgaa ttttcttagc ggctccgtaa tacaaatctt ttaggatttc ttcagctgtt
    30181 tcttctttag aaacaagccc tgcgatttga cctgccatga cagagccacc ctccacatca
    30241 ccgtgaacaa ctgctttggc tagggcacct gctcccattt gttcaaagat ttctaaatca
    30301 ggatcttcct gcttaaaggc atctttttca gccagttcaa aatctctagt caactgattt
    30361 ttaatagcac gaacagcatg accaaagtgc tgagctgaaa tcgtagtatc aatatccctt
    30421 gcttttaaaa ttttctcttt gtagtttgga tgggcattcg actcttttgc aactacaaac
    30481 cgtgtcccca cctgtacagc ctctgcacct agcataaagc cagccgcagc accttcacca
    30541 tccgcaattc ctcctgcagc aataacagga atagatatag ctgtggctac ctgtcgcacc
    30601 aaggtcatgg ttgttaattt accgatatgc cccccagctt ccattccttc tgcaataaca
    30661 gcgtctgcac cgattttttc catgcgttta gctaaagcga cactaggaac aacaggaata
    30721 acgattatcc cagcttcatg gaaacgttcc atatacttgc ttggatttcc tgctcctgtt
    30781 gtgacaactt taacaccttc ttcaataacg agatccacga tatcttccac aaagggagat
    30841 aagagcatga tgttgacccc aaagggttta tcagtcaatg atttgatttt atcaatattg
    30901 gccttgacaa cttctttcgg ggcatttccc ccaccgataa ttcctaatcc tccagccttg
    30961 gaaacagccc ctgccaaatc accatcagca acccaggcca tccctccttg gaaaatagga
    31021 tagtcaatct tcaataattc tgtaatacgc gttttcatag tgcctccaac cttccttgct
    31081 tacgtaatag ttcgatttca ccataatttg acagtcaaac tattacctaa acaagaggga
    31141 gtgggtttct ccctactcct tctactaata ttctgcttat tttgcttgct cttcaacgta
    31201 agcaaccaag tcaccaactg ttttcaagtc attttctgct tcgatttgga tatcaaaagc
    31261 atcttcgatt tctgagatta cttggaacaa gtccaatgaa tctgcgtcca aatcatcaaa
    31321 agttgattca agtgttactt ctgatgcgtc ttttccaagt tcttcaacga taatttcttg
    31381 tactttttca aatactgcca tgataggact cctttaaaat aaatagtttt tttataacaa
    31441 tgtgttcacc acatgattac ctaaattgta agaatgagcg tgccccaggt caagcctcca
    31501 ccgaagcctg atagaagaac agtttggcta ccatctaaag ggatgagacc ttgttctaca
    31561 cactctgaaa gtaaaatcgg gatactggct gcactggtat tgccatattc catcatatta
    31621 gctggaagtt tggctcggtc aacaccaatt tttctagcca tcttatccaa aatacggtta
    31681 ttggcttgat gaagtagcag ataatccaag tctgtcacct ctataggaga ttcatcaata
    31741 gtctgcttga tagacttggc tacatctcga atggcaaaat caaagactgt gcgtccatcc
    31801 atcttcaaaa atgaatctgc actttcttga tctgaaaatg gagaatgcaa acctgaatgc
    31861 ccataagtta aacactcgct gcgacttcca tcgctattga gactctcagc taagaaatgc
    31921 tcttgctcgc tagcttctaa caagacacca ccagcaccat ctccaaacaa cacagctgtt
    31981 gatcgatccg accaatcgac tgccttagag agggtttcac taccaatcac caagcctttt
    32041 tgaaagcgac cagaagcgat aaacttttca gcagttgaaa gagcaaatac aaatccactg
    32101 caagccgcgg ttaagtcaaa agcaaaggct ttattagcac caatattagc ttgaacacga
    32161 gcagctgtag agggcatcat cgaatctgga gtaatggtag ctaggatgat aaaatccagt
    32221 tcttctcctg ttattccagc ttttgccatc agtttcttag caacctctgt agccaaatca
    32281 ctggtagatt ctgttcttga aatatgcctt tgtcgtattc ccgttcgact tgaaatccac
    32341 tcatcattgg tatccataat ctgagccaag tcgtgatttg taaccacttg ctctggcaca
    32401 taatgagcaa cctgacttat ttttgcaaaa gccattattt caaatcctcc aaaaattggt
    32461 aaagattagt caaaccttta cccatgacag caatttcttc ctcgctcatg ccatcaataa
    32521 ttttttctac catggccttg tggaagcgtt tatgcagtct atgaatcaag cgacccttct
    32581 ttgtcaaatg cagatgcacc acacgacgat cctgttctga ccgaactcgc tcaatgtagc
    32641 ccttacgctc taaattgttc aaacttgtcg taacagttcc aagagttacc atcaactctt
    32701 ttgacacttg acttggagtc acatctggag ccttaccgat gacatcaatc gtatgcattt
    32761 ctttgatgga gatatcctta aaacgactac ctctcaagtt cacttcctca attacaagga
    32821 cattgttaaa tatagatgtt aaatattcat tgattcgttg gtagtccatt tttcatatcc
    32881 ctccttcttc aaaaaacttt tacaatcaaa acatttgaca gaaagaatta tatcaaaatt
    32941 caaagaatga tgcaagtatt ttttattttc ctgtaaattt aggtcttctt ctctccgaat
    33001 gagctcgcac tccttctttg aaatcctctg tttgagctaa ggatttctgt aggttcagtt
    33061 ctaaagtagc gtaaccctgc cagtctttaa attggctctc ccaaaccaac ttcttaatgg
    33121 cagcatagga attacttgaa gcacgtctta attttttaag aagctgttct ctcgtctttt
    33181 caagtttttc agcttcagaa acgcggtaaa ccaaccccca ttctagggct ttttctgccg
    33241 ttaaagcctc tcctgtcata gctaattgag cagcacgcgt cacaccgata ctgcggctca
    33301 agagatgaat tccccctgca tctggagcca aaccaacacc aacaaaggct tggataaact
    33361 tagccttatc cgtcgccaaa cagaaatctg ccgcaacagc catattcgct gcggcacctg
    33421 caacagcacc gtcaacttcc attaaaacag gtttggctat ttgcttgatt ttataagaaa
    33481 tcgtattgac caactctgcg atttttgtca atgatggaat atcatcctca tccactgccc
    33541 gcttcatctc taccaaatct cccccaactg agaagacctt tccattggca ttgattaaga
    33601 taaaatgcac agctggattt tcttctgcca aagtcagagc ttctaaaatc tcctcacaca
    33661 tgggaatatg aaaaccattt gcgacctcag gacggttcaa ggtaaggatt gccaagtcct
    33721 cttcaagctg ataaataatg tgttccatca gggctccttt ttattttata agcagtttat
    33781 actcttcgaa aatctcttta aaccgcgtca gcttccatct gcaacctcaa aacagtattt
    33841 tgagctgact tcgtctagct tcttagtttg ctctttgatt ttcattgagt gttaaattat
    33901 ttcactgtca aagtatatct tttttaggag cagaaagcaa gaaaaaatcg actgaaagtg
    33961 tctcagccga taagaaaggc tcgctttagg ctagaaagca tcttattttc ttcattgaaa
    34021 aaagcctttc tttctgctat aatcgtataa aatacttact ttaggagttc ttatgaaagt
    34081 tgttaaattt ggaggtagtt ctcttgcctc tgctagtcaa ttagaaaaag ttttaaacat
    34141 cgtcaaaagc gattcagagc gtcgttttgt agtcgtttct gcgcctggta aacgcaatgc
    34201 tgaagatact aaggttacgg atgccctgat taaatactac cgcgactatg ttgcgggtaa
    34261 cgatattagc aagagccaaa gctggattat cgaccgctat gctgctatgg ttagtgaatt
    34321 gggactaaaa ccagctgtgc tagaaaaaat ttctaaaagc attcacgcct tggccactct
    34381 tcctattgaa gaaaatgaat ttctctacga tactttccta gcagccggtg aaaataacaa
    34441 tgccaaattg attgctgcct actttaacca aaatggtatc gatgcacgct atatgcaccc
    34501 tagagaagct gggattgtgg tcacaagtga acctggtcac gctcgcatca ttccatcaag
    34561 ttatgacaag attgaagaat tgacaaacac caatgaagtc cttgtcattc ctggtttctt
    34621 tggtgtcact aaggaaaatc aaatctgtac tttctcacgt ggaggttctg atattacagg
    34681 ttctatcatt gctgctggtg tcaaagctga cctctatgaa aactttacgg acgttgatgg
    34741 tatctttgca gcccaccctg gtattatcca ccaaccacac tcgattcctg agttgaccta
    34801 ccgtgaaatg cgcgagttgg cctatgcagg cttctcagtc cttcatgacg aggctcttct
    34861 tcctgcctac cgtggaaaaa ttcctctggt tatcaagaat accaacaacc ctgaccatcc
    34921 aggtactcgt atcgttctaa aacacagtaa tgatgaattt ccagttgtgg gaattgctgg
    34981 tgactcaggc tttgtcagca ttaacatgtc gaaatacctc atgaaccgtg aggttggatt
    35041 tggccgcaag gttctgcaaa tcctggaaga acttaacatc ggttgggaac atatgccaac
    35101 aggtatcgac gatctttcta tcattctccg ttctcgccaa ctaactccca tcaaggaaga
    35161 agaaatcctg cgtcagttgg ttcaaaaggc taaagtagat catgcagaaa tcgaacacga
    35221 cctttctatc attatgattg ttggtgaaaa aatgaagagc catatcggag tgactgctac
    35281 tgcgacacgc gctctatctg aaaacaaaat caacatccag atgatgtctc aaggttctag
    35341 tgaagtttct attatgtttg ttgtcaataa agaccaagag aaagcagcaa ttaaagccct
    35401 ctacaatgcc ttttttggag aaagtaagga agactaagca tggcccaatc acttaacaaa
    35461 acagtactcc tcagtacgac aggcacttcc tacctctcta tcgctgggaa agttgggaaa
    35521 ttccttgtcg gagatcaggc tttggaattt tacccagatg tcaatgtcga acaatttatc
    35581 cagattcctt ggagccatat caatcagatt ggagccaatg tcactggacg caaaatcagc
    35641 cgccacttcg aagtctttac agacaaagga aaattcctct ttgcttcaaa agactcaggt
    35701 gccattctca aaattgcacg cgaaaaacta ggcaatgaca aggtcgtcaa acttccgacc
    35761 ctgattcaga caattagcca aaaatttaaa aatctatttg caaaaaagta gaaactttag
    35821 tataatcata gcaacggata agtaggttat cttcttcttt cagaaagtct gcgggtactg
    35881 cgagcagata ggggggatag gtgaaattct accgttagta acaattacat acacaatcaa
    35941 gtgcacacct gtgaagttgg atggaaccgt ggccctgcca ctccaacgtt ttgtcaggcg
    36001 tgcttttttc ataaaggagt tcttatgtta gatatcaaac gtattcgtac agattttgaa
    36061 gctgtcgcag aaaaattagc tacacgtggt gtagatgctg ctgtcttgaa tgaaatgaaa
    36121 gaaatcgatg ctaaacgtcg taacatcttg gtcaaggttg aaactctcaa agcagaacgt
    36181 aacacagttt ctgctgagat tgcccaagct aagcgcaaca aggaaaatac agatgacaag
    36241 attgctgcca tgcaaaatct atctgctgag gttaaagcct tggatgctga attggcagaa
    36301 atcgatgcta aattgacaga atttacaacg actcttccaa atatcccagc tgacagcgtt
    36361 cctgttggga ctgacgaaga cgacaatgtg gaagttcgcc gttggggtac tccacgcgag
    36421 tttgacttcg aacctaaagc tcactgggat ctcggtgaag accttggtat ccttgactgg
    36481 gaacgcggtg gtaaggtaac aggcgctcgc ttcctcttct ataaaggcct cggtgctcgt
    36541 ttggaacgtg ctatctacaa ctttatgttg gatgaacatg gaaaagaagg ctatactgaa
    36601 gtcatcacac cttacatagt caaccatgat tctatgtttg gtactggtca gtatccaaaa
    36661 tttaaggaag atacttttga actcagcgat accaactttg tcttgattcc aactgctgaa
    36721 gttcctctga caaactacta ccgtgatgaa atcttagacg gcaaagatct tccaatctac
    36781 ttcactgcca tgagtccgtc attccgttct gaggctggtt ctgccggtcg tgatacgcgt
    36841 ggcttgatcc gtttgcacca attccacaag gttgaaatgg tcaaatttgc caaaccagaa
    36901 gaatcttacg aagaattgga aaaaatgaca gccaacgctg aaaacattct tcaaaaactc
    36961 aaccttccat accgtgtcgt tgctctctct actggagata tgggcttctc agctgcgaag
    37021 acttacgact tggaagtgtg gattccagca caaaacaatt accgtgaaat ctcaagctgt
    37081 tcaaacacag aagatttcca agcccgtcgt gcccaaatcc gttaccgtga tgaagcagat
    37141 ggcaaggtga aactccttca taccttgaac ggctctggac ttgcagttgg acgtacagtg
    37201 gctgcaattc ttgaaaatta ccaaaatgaa gatggttctg tgaccatccc agaagcactt
    37261 cgtccataca tgggtggagc tgaagtcatc aaaccataaa aaataaggtt tagctatttc
    37321 tagctagacc ttttttcgta accaaatcag ataagcacct agtacaaaga ataaaatagt
    37381 taggcatata atggtttcag ccaataccag gtaatccaga aatggaagtt tcaaaattcc
    37441 ctgagccatc ttgagcgagg tcgctgtgat aatggttggg aaggtgaggg ctgagaaggc
    37501 tggttgaaaa ccttgtttta aaatgttggg cagacgagtt aaaacaaaga aaaagaagga
    37561 ttgagaagcc aaaatcatga caatcaagac ccaagtcggc aggctggttc ctcctactcg
    37621 aactagagaa gccaagagta gagagaaagg agcacagtag attccttctt gtccaagcaa
    37681 ggctagtggg agtggatgtt tctttaaatc gctataaata aggggataga gatagaaggt
    37741 caagagaaaa ccaaaactca aggtcgcata ggcaatttcg ataataccta ccagaggata
    37801 ggtcaaggca gccactgcta tccccacata gagaaccgtc caacttggag tggcatgaac
    37861 cctccgccct gaacaagcaa acttgatggt aaaaccagca atcaaggtca aatccaagag
    37921 aaatgaaaac caccaaatcc cttgtgctac caaaggaaga taagagaata cgcgaaagac
    37981 ataggtcgat aaaatcatcc cagccatagg aaaggttgcc attcctgaca aaagaggggg
    38041 cttggtcaat tcttgcttgg tttctttcca attaaagaga tgcagaatta gaaagtaaat
    38101 ccataaaacc aaaccagtca gactaaaaag atgggataga accggcaacg tatctaaaat
    38161 aagatttcca gctcctgcca aacctagcaa acaacctgaa aatactaagg ggagtttttt
    38221 catcctaacc tccaataatc atgttagttt cagtataaca taaaagcgct taaatgagga
    38281 tttaaaaaaa cgaagtccgc ttatttcaga cttcatttta ctcagatatg aattaggcat
    38341 aaggttgcaa ttctggatta attggtgtat tagctaagtt gttggcatag ttacagagga
    38401 ttgctaggct gacaccaaaa accacatcca aggcattttg ttgagtgtag ccagcttcta
    38461 aaaactcaga caaggcttca tctcctacac gacccttggt attgataact gccaaggtaa
    38521 acttagctag ggtatccaat ttaggatctg tttcaattgg agtacgattg cgaagagctt
    38581 gaatcaagtc atcattcatc tggatttgtt tgatggaaaa ggctgtgtga cctgcgacac
    38641 agaaggcaca accattggtc acggctgccg tgatttgcac cacttcacgc tcaacgggtg
    38701 tcaggctgtt gcgacggtgg atagatgaga caatttggta ggcttctaaa acagtcgggg
    38761 cattggccaa gagaccgatt aggttgggaa tatagccatt gttgtctttt tctactgttt
    38821 caagaatttc tttcacttct gctggtgctg actctactgt atggatagta aatgttgtca
    38881 taagatacct cttttcttat tattgacact aatattattg gaaaatctta taaaatcctg
    38941 attcctaagt ttatctaaga taaagcttta ttctctcata agattttcgt tgttatatta
    39001 gtttatcaca cttccaatca cttgcataat atatattata tatcaggctg ataaaaatta
    39061 tttataggca aaaaaatcac acgagctgtg tgattccatt atttgtcaaa atacttttta
    39121 gtttcagcaa taacgactgg cgacaagacc aagagggcaa tcaagtttgg cagagccatc
    39181 aaggcgttaa cgatatctgc gataatccag accatatcca actcgataaa tcctcctaac
    39241 aagaccatga gcacaaaaaa cacacggtag agccagataa agcgagcccc aaagaggaac
    39301 tcaaaacagc gttctccgta atagttccaa cctagaatcg ttgtaaaggc aaaaagtaca
    39361 aggaagatgg tcaagagggc aggcccaaag tgtgaaaaga ctgttgagaa agctgactga
    39421 gtcaaggcaa ccccattcaa gtcaccactc caaactccag ttaccaagat ggtcaaacca
    39481 gttagagtac aaatgatgag ggtatcaata aaggttcctg tcatggaaat caaaccttgc
    39541 tctactggtt catttgtctt ggcagctgca gctgcaatag gagcagaacc cagaccagat
    39601 tcgtttgaga acacaccacg cgccacacca ttttgaatag ccatccgaac gctagcacca
    39661 gcaaatccac ctaccgcagc aaggggacta aaagctgagg taaagactaa agcgattgtg
    39721 ccagggattt ttccgatatt aaagaaaata actgtaagag ttcctaagat ataaatgagg
    39781 gccataaaag gaacaacagt agttgaaacc ttagaaatag acttgagtcc accaaagact
    39841 gcaatcgcta caaagacaga caagacgaga gctgtgatgg ctggcgaaat cgtcgttgta
    39901 ttttggatag attctgtaat cgagttgact tgggtgaagg ttccgattcc caagagagca
    39961 accaatactc ctgctactgc aaacaaaaca gcaagtggtc gccacttttc tcccatccct
    40021 agaaggatat agtgcatggg acctcccgct actgcaccat ggtcgtcctt ggtgcggtat
    40081 ttgatggcca agagtccttc cgcatacttg gtagccattc caaagaaagc cgccatccac
    40141 atccaaaata gagctcctgg tccaccaacc ttgatagccg tcgcaactcc tatgatattt
    40201 cctgttccaa cagttgatgc caaggctgta cacagagctg caaaactgga tacatcacca
    40261 tgtcccttat cctggataaa aataagctga aaggccttgg gcagacgcaa aacctgcaag
    40321 agtcctagcc gcatggttag gtaaatccct gttccgacca ataaaatcaa gaggggcggt
    40381 ccccaagcaa aagcatcgat tgatttaagc aattctaaca tttccttctc ctatcgtttc
    40441 aaccccaaaa gaaagagcac atgcaagata catgtactct ggaatgctta gataaatgct
    40501 aaaaagcggt ctatcctagc tctgtccttt tacctgagag tttgagcagt tgcctgcctt
    40561 gccccttcgg tgcctttacg gtctctccag agttccgtcc atttacagtc atggaaaatc
    40621 aaacgattcc ccacttctat taaacttcat tcggtgttgg tatttaattg attctaattt
    40681 tacaaaaaat gttggctttt gtcaatgtgt ttattagtaa aaattagttc aacagttttt
    40741 actttataaa gtccagaata ctgctatcct ttaaaagtga caatagtcgc accactgcct
    40801 ccagcatttt gtggggcata gccgaaactc ttgacatgtt tgtttctttg caagtatttg
    40861 gtaactcctt cacggatgac tcctgttccg ataccatgga tgatatcaac ttgagccata
    40921 ttgttaagca aggcttggtc gatgaaggta tctagctcat tcatggcttc ttcatagcgc
    40981 ttgcctcgaa gatccagtct agcttgaggt cctcgcccag aagttcgttt cacaacattg
    41041 acctgtttct tcttgactgg tttttcttgc tgggcttgaa caagatcaaa ctctttctct
    41101 tccaaggtca tcttaatcaa gccaacttgg gcttcccagc gaccgtcctt gagttgactg
    41161 gtcaaggtac cacgctgacc ataactgaga accacgatat catctcccac ctttggagct
    41221 cgttttttct tggccttttg aaggacctta tttttagaca agtccacttt ttcaggagcc
    41281 aattttttca acttggcctt ggcttcaatg atttcgtggg gcttgagttg ggatttactg
    41341 tggagatttt tgagaatctg gtcactttca cttagggcca tatccacaat ctcagcagcc
    41401 tgttcacgcg ccttgttaag ctcggtttcc ttttcacgat taagctcgtt gtagagtttt
    41461 tttagcgcac ggttcatctt gagattttct tgctccacct cacggatatt gtccaaacgt
    41521 ttgcggcttt ccagcgtctg ctcttctaat tgctcaatga tacgattgac gtcattgtcc
    41581 tgatcgatct gctgactggc atctcctacg ataacttcag ataggcctag acgtttggca
    41641 atttcaaagg cattacttcg gccaggaaca ccctgcataa agcgataggt cgggcgaaga
    41701 gttgcagtat caaactccat actggcattt tgcacaaagg ctgtctcaat accgtaggcc
    41761 ttgagttctg gatagtgggt cgtcgccatg gtcttgattt gacgcaggcg aaggtcctcc
    41821 agaatagcca tggcaagggc tgctccctct tggggatcag taccagcccc caactcatcc
    41881 aaaagtaaga gtgaatgttg gttgaccttg ccaagaatat ccacgatatt ggtcatatgg
    41941 ctagagaagg tagacaagct ctgctcaata gactgctcat ctccaatatc agcaaagatt
    42001 tcttcaaaaa taccaacacg acttcccttg tctgctaaaa tcggcaatcc tgactgggcc
    42061 atgacctgtg tcaagcccag agttttgagc atgatggtct tcccacctgt attgggacct
    42121 gtaatgacaa tagctgttaa atcttgacca aaatggacat catttgcgac ggcatttttg
    42181 accaaaggat ggcagacatg gagcagttga atctcttgat tttctgacag ctgaggcacg
    42241 actgcttgtc tttcttggat aaatcgaacc ttggcacgaa tcaagtccag atgaccgata
    42301 atccaagcgt cattagcaat ctcagccgca tgagggcgga cacgctcaga aatttcttgg
    42361 agaatgcgaa gcatttcata gcgctcatct gctcgcagac tagcaatttc ttcgctcagt
    42421 ttgactacct cacgtggttc gatatagacg gtgtttccac tagcagaaat atcatgaacg
    42481 acacctgcaa tcttattgcg gtaggtgttt ttgactggta aaacctgacg gccatttctg
    42541 ctagcaacaa ttccttccgt caacatctgc gctttttgct tgagcaagtc ttgtaaaaca
    42601 tcgcgtacct gactctcgct atcatgtatt tttcgacgga ttcgcgccaa ttcttcactg
    42661 gcaaaatttt caatgaaacc cgcatcatta aaggcctgaa gatttccttg taattgcgga
    42721 aaatcatgta atttctcaaa ccaaagggct aattcttcca agctgacatt ttccagattg
    42781 gcataaaaac tttgaagttc tcggctggca agaagcacgc gtttcaagag taggaactcc
    42841 tcgatattga gatccgctcc catctccaac ctcttgcaga ctcctgcaat ttccttagtt
    42901 gagagaatag taaaatgcgg ttgctcgacg aaaagagcct gcatttcttt catctcagca
    42961 aaagcctgtt tgattttatc tgctttggca gtcggagcca gctgtctcaa ttgctccaag
    43021 ccctgctcgg tcaacaaatg aggatcaaac aaggccttga ccttatcgaa ctctaatgtt
    43081 tctaatattt tcttattcat ctttatttcc ttgtctttga atgtctaggt gtggtagctt
    43141 tttacattct gatagattcg agtcaaatct gtaaacaaag ggctaggaaa actcctgccc
    43201 tttttatccg attaaatttg tcacccagat ttgtttgagc caactggttg ttatcggtat
    43261 gctctggatg atgtgttttg cgacgatact cttttcaaga ggattttgta taactgccat
    43321 ggggatggtc gccaagattg tcaaggccat ttgcaagaca aataaggtca ccaacatgga
    43381 caagatacct gctgaaactt ggaacaactt accacccagt tttttgctag gaatcaagtg
    43441 taagagaaga ccaagcaaac gaccaatgct atagaaaatc ccaaatacaa gcaagtagcc
    43501 gatacctgca taaaagacct tatccagctg aaagagttga tccgatggga aaaagaaagt
    43561 cccctgacct tcctgcgaat ttgcataagg gagcaataaa tggaattgct ctccaagccc
    43621 cttataaaac tggccagcca taaaagccga tgccatggct gaaatcaggt aataaacctg
    43681 taagagcagg cctctccgat agccgatata aaatccccaa accaagacca atagaagaag
    43741 gaatgaaatc ataaggaatc ctcaatcttg ctctgttctt gcttacaagt cacaagcttg
    43801 tgacggagtt cttctagctc ttgctcctta tcgtcaaatt caatctcacg gctgagctga
    43861 gttgataaac agttgactgc caacaaaaga gcgattgttt catcatctgc gctaggcatt
    43921 tgttctttaa ttgcttggta tttttctgtc gcaaccttag cgatttcctc cataaaaagg
    43981 ttatcatgtt cgcttgtcaa ggttaacgat tttttcccga atgtaaattt gaatcgattt
    44041 agatttgcca taaaattcac ctcacgatat tataccaaaa ttcgctaatt ttgtcagttt
    44101 ttacaaattt tactgctttt gtggtacaat agaaactatg gcaagtatca cactcacacc
    44161 aagcgaaaag gatattcagg cttttcttga acactatcaa accagtctgg ctcccagtaa
    44221 gaatccctat atccgctact ttttgaaact acctcaagca acggtttcta tctatacttc
    44281 tggaaaaatc ttgcttcagg gtgaaggggc tgagaaatac gctagtttct ttggctatca
    44341 acttgtagag gaaaacaggg ggcaaaatct tcctttaatt gggacagatg aggtgggaaa
    44401 tggttcctac tttggtgggc ttgcagttgt ggctgccttt gtcacatctg accagcacga
    44461 ctttttacga aaactcggtg tgggggattc taagactctg accgaccaaa agatccgtca
    44521 gattgctcct attctcaagg aaaaaatcca gcaccaggca ctccttctct cacccagcaa
    44581 gtacaacgag gtcatcggag accgctacaa tgctgtttcg gttaaggttg ccctccataa
    44641 tcaggctatc tatctcctcc ttcaaaaagg tgttcagcct gagaaaattg tgattgatgc
    44701 ctttaccagt gctaaaaatt atgacaagta cttggcacaa gaggccaatc gtttcagcaa
    44761 tcctatcagc ttagaagaaa aggctgaggg caaatacttg tctgtcgcag tttcttctgt
    44821 cattgcgcgt gatctctttc tggaaaatct tgaaaatttg ggacgagaac tgggttatca
    44881 gctcccaagt ggagctggaa cggcttctga caaggtggct agccagattt tgcaagccta
    44941 tggtatgcag ggactcagct tctgcgccaa attgcacttt aaaaacactg aaaaagcgaa
    45001 aaaacgctta gaaaggtaag ttatgaattc atttaaaaat ttcttaaaag agtggggatt
    45061 gttcctccta attctgtcat tactagcttt aagtcgtatc tttttttgga gcaatgttcg
    45121 cgtagaagga cattccatgg atccgaccct agcggatggc gaaattctct tcgttgtaaa
    45181 acaccttcct attgaccgtt ttgatatcgt ggtggcccat gaggaagatg gcaataagga
    45241 catcgtcaag cgcgtgattg gaatgcctgg cgacaccatt cgttacgaaa atgataaact
    45301 ctacatcaat gacaaagaaa cggacgagcc ttatctagca gactatatca aacgcttcaa
    45361 ggatgacaaa ctccaaagca cctactcagg caagggcttt gaaggaaata aaggaacttt
    45421 ctttagaagt atcgctcaaa aagcccaagc cttcacagtt gatgtcaact acaacaccaa
    45481 ctttagcttt actgttccag aaggagaata ccttctcctc ggagatgacc gcttggtttc
    45541 gagcgacagc cgccacgtag gtaccttcaa agcaaaagat atcacagggg aagctaaatt
    45601 ccgcttctgg ccaatcaccc gtatcggaac attttaagag acctaagagg ccgagaatca
    45661 ccaatctcag cctcttcttc tatcgtgaga agtgattgtt tattgacaaa tactcaacga
    45721 aaatcaaaga acaaactagg aagctagccg taggctgctt aaaacagtgt tttgaggttg
    45781 tggatagaac tgacgaagtc agctcaaaat actgttttga ggttgcagat ggaagctgac
    45841 gtggtttgaa gatattttcg aagagtataa aataaaaaca atcccaactc tcacctattc
    45901 atgcaaagga attttatgga agtttatttt tcaggaacca ttgaacggat tatttttgaa
    45961 aatcccagca atttttatcg catcctcctc ctagaaatcg acgatacgga cgcagaggat
    46021 tttgatgatt ttgaaatcat tgtcacagga accatggctg atgtaattga gggcgaagac
    46081 tatacttttt gggggcaaat tgtccagcac tccaagtatg gagaacaact gcaaatcagt
    46141 cgttatgatc gcgcaaaacc aactagtaag ggcttggtca agtacttttc aagtagccat
    46201 ttcaagggga ttggtctcaa gacagctcag aaaatcgtgg atacctatgg cgaaaatacc
    46261 attgacgaaa ttttgcaaca cccagaaaag ttagaaggca tcgcaggact ctctgccaaa
    46321 aatcgcgagg ctttcgtctc cactctccgt ctcaactacg gaacggagat ggttttgtcc
    46381 aaactagcca actacggcat tcccaacaaa ctagcctttc agattcaaga cttttacaag
    46441 gaagaaaccc ttgatgtggt tgaaaattat ccctaccagc tggttgagga tatcaagggt
    46501 ttgggcttta ccattgctga ccaactagct gaggaactag gcatcgaaag tcaggctcct
    46561 gaacgcttcc gcgccggtct agttcacagt ctttttcagg cctgtatgga aacaggggac
    46621 acctatgttg aagcacggga tttgctggaa caaaccctta ctctccttga gtcttcccgt
    46681 cccgtggaac tagaccccag ccaagttgcc caagagctct cctacctgat cgaagaagac
    46741 aaggttcagc agattgatac caagatcttt gacaacagcc tctttttcgc tgaggaaggc
    46801 atccgcagtc acttggttcg tatccttgaa aaaggaaaac agaagagtca tgatttagaa
    46861 accattcaaa aacatatcac tactatcgag gaagagctgg gaattgagta tgataacatt
    46921 caaaaacagg ctatctgtga tgctatccag aataaggtct ttatcctgac aggtgggcct
    46981 ggtactggta agacaactgt tatcaatgga atcatcgctg tttatgccct gttagaggga
    47041 cttgacttca ggaagaaaag caatctgcct attcttcttg ctgctccaac tggtcgagct
    47101 gctcgtcgca tgaatgaatt gacaggtttg cctagcgcga ccatacatcg ccacttggga
    47161 atgacaggtg acgatgatac cagtcatctg gaagattacc tagatgctga ctttatcatc
    47221 gtggatgaat tctctatggt agatacttgg ctggccaatc aactcttctc caatatctct
    47281 tctaacagta agatcctcat cgtaggtgac agtgatcagc taccttctgt cagtcctgga
    47341 caggttctag cggatctgct tcatattcct ttgattcctc agactcactt ggaaaaaatt
    47401 tatcgacaaa gcaaagaatc aaccatcgtc accctagcta gtcagattcg acagggcatc
    47461 ttgccagctg atttcaccca aaaaaaagct gaccgttcct actttgaaat tgctagtggc
    47521 catattcctg ccaccattga aaaaatctta ggtgccgccc tcagaaatgg tattcctgcc
    47581 cgtgatatcc aagttctggc tcctatgtac cgagggacgg cagggattga tgctatcaat
    47641 cagctcatgc aagacctgct caatccccca caaaaagatc aactcagttt tgaagctcct
    47701 cagtgccact atcgtaaggg agacaaggtc attcatttgg ttaacgatgc tgaaatcaat
    47761 gtctttaatg gggatttagg agctatcaca gacctgattc ctggtaaata caccgaatcg
    47821 aaacaagacg agattgtcat tgattttgat ggtaatgaag tctcttaccc ccgtaacgaa
    47881 tggtacaaga ttcgcttggc ctatgccatg agtattcata agtctcaggg aagtgagttc
    47941 ccagttgtca tcctaccgat tactagtgct agccgacgta tgctggagcg aaatctcatc
    48001 tacacagcca ttacacgtgc caaaagcaaa ctcatcttac taggcgaatt acaagccttc
    48061 gactatgcta cccaacacat cggaactgcc cgaaaaacct atctgattga acgcttcagt
    48121 gatctactgg agaatgttga agaaaagcaa caagctgtct ctgaaacagt cacatcaagt
    48181 gcctctgaac aatcctacat cctaaccgaa gaaaactggg acagcattcc agccatgatt
    48241 gggattacag acacagacct caaagagatt tttggaaaat agtgcatcag aaaaaccacc
    48301 aattcaggtg ggttttctgt ttattaagat tattttactg ttgctgtgct tgtaatcaat
    48361 tcaacagctt ttttgatagt gatatcatgt ttcaacatgt cagctgaaag caagttttga
    48421 acttgtgcaa cttccatgtt gtagtctgct gccaattgct caacttcttt ttggatttct
    48481 tcttctgaag catcaaatcc ttcagctttg gcaactgctt cgataacaag gttagtctta
    48541 gtacgtgact cagcttctgc ttggtattgg ttgtgaaggt cttcttgagt agttccagta
    48601 atttggaagt acatgtcagg gttgatccct tgacgttgca agttcccaag gaattcattt
    48661 actgaacggt gaacttcttc gtggatcatt tcttctggaa gttctacgat ttcagcattt
    48721 tctacagctg tatcaattgc tgcaccttca actgcatctt tgtaagcttc ttctttagca
    48781 gcagccaatt ctttgcggta tttttctttc aagtcagcaa gtgtttcaac ttcttcatca
    48841 atgtcttttg caagttcatc gtcaagagct ggaacttctt tagcttttac ttcgtggata
    48901 gttgtcacga atttagcttc tttacctgca aggtcttctg cttggtagtc ttctgggaat
    48961 gttacgataa catcaacggt ttcgccagct gagtgaccta ccaattggtc ttcgaaacca
    49021 gggatgaatt gacctgaacc aagtccaagt gagaagtttt caccttttcc accgtcaaat
    49081 tcaacaccgt cgatagaacc aacgaagtcg atcacaacag tgtcgccgtc ttcagcagca
    49141 gcttccttga taaccaattc agccaggttg ttgcgttcac gttcgatacg ctcttcgaca
    49201 tcagcgtcag ttacttcttt ttctacatca actgatactt caaggttttt gtagtcaccc
    49261 aattttactt caggttttgt aacaacttca gcagcgataa cccagtcttg acctttttcc
    49321 attgaagtta cgtcaatttt tggttgggca accacttcaa gaccagcctc ttttacagct
    49381 gcttcataag cgtttggcaa aagagcgttc ataacgtctt ggtaaagtga ttcttcacca
    49441 aattttttat cgaagatagg acgtggaagg tgacctttac ggaaacctgg aacattaaga
    49501 gatttcttca ctgacttgaa gacacggtcc aattctggtt tgatttggtc ttgagagata
    49561 gtgaaagtca agacaccacg gtttgtttct ttgttttcaa atgatacaga cattctgtca
    49621 tttctcctta aaatttttta aatacagtct attataacat tttctataaa taaaatctag
    49681 actaatgcag acaattatag acaacaagcc tttttcctaa ctaaaacact cataaagtat
    49741 agacaatttt agactacttt ggatatcttt tggttagtca ttttccaaaa ctaatttatc
    49801 taatttttaa gttatatttg acttcatctt ctcagtaaca tgagtataaa cacttaaaat
    49861 ctttgtttca ttagtataat ctacacgatc cattatcgct gttataggta cttccaattc
    49921 agcaagcaaa cttatatgcg aatatctaaa aacatgagca gtcaatacaa aatcatcttt
    49981 cttaatttca cttttcctaa acctgttttt caaaaagtaa ttgaaacaag ttatcattaa
    50041 gggagaacca ttatttgtaa atatataatc accttcttct aaaagaagat attcttttaa
    50101 aatttctaac actctatcac taataccaat tactctatat gaagctaaac ttttcggact
    50161 atccaaaaac acttcgttat tggacgatta cattatagta aaacgaaata agaacaggac
    50221 aaatcgatca ggacagtcaa atcaatttct aacaatgttt tagaagtaga ggtgtactat
    50281 tatagtttca atctactata caagacgaaa ttgaagaaat ctttccatta taaaacgcat
    50341 attatcaagg tttttaaaca cctaataata tgcgtttttt cctattttaa agacttttat
    50401 ccacctatta tacaaagtta agtatccatc acagtttaca attagccttc attacctaac
    50461 ccattcacca tccttcaaga gagtaacctt taagaaccga ataaataaat ggcaaatttc
    50521 gttcatttaa tttaactgca ctgttaattt gattagctcc atctcctaaa gtatgcactc
    50581 ctactacacg gtgactagca tcataaactg tagatccact agacccctct aaagtatcaa
    50641 cttggtaatc caagaacatg ccatcatcac ttaaaacttg ttttttatct gtatacattt
    50701 gatgaacttt aaaattatat gatggatagc ctgtgatagt cacagttatt cctgtcaaat
    50761 ttttttgact agtaggaaga cccaaagtcc ctaattttgc accaatgggc tcttctagaa
    50821 ttaataaagc caagtcatat tcccttgcat ccttagaatt taaatttcta aattccttca
    50881 aataacgaac ttcctttact ttgatctttc caaatggttc ttgacttgga ctaacagtcg
    50941 gaagaacata aatatcatcc gcttctttcc catagtcatg tctgtagtag ttgtgagccg
    51001 ctgtaattaa cacattatct ttaataaaat tcgcacttcc cctcccttct acattcattc
    51061 catcggctac tccataatac ttagataaaa ccatagctga agtcgaatac ggatactgta
    51121 aagtattatc aattttaatc aaatcatcat taccgataat acttctgatt gcttttggta
    51181 gtatgaacca tatgttggtg aaatctcaga taatgaagaa tcattagatc ctggaccttt
    51241 ttctagtgtc tcacttacct catattcttc acccgtacta gaaataacac tcaaagcaga
    51301 tactgtcgat aactggctag ccaataaagt actcgcaata attgaaatac ccaatttttt
    51361 ataaacagtt ttcttcatta ttgtatcctc ctaatgtaat tatagcgtac tattctaaat
    51421 ttcttaatct actatagaat caagaaatct accaccttct ttaaataccc tccattatca
    51481 cataaacagg taaacttttc aattaatgac tgcgcttttc aatcacgcta gaggtactta
    51541 cttgcttctt tgatactaag ttcagccatt ctttccttgt ttttctcaat aaagcatgtt
    51601 acccaagtgg gattcgtttt ggagtagtct cgcagagtcc agccaatggc tttattgata
    51661 aaaaattctg tttggttcaa gttatgaagt agaatctttt ccattaattg agtattggtc
    51721 ttctcttttc ttaacaactg gtggtcaata gcgacacgtc tcagccagat attatctgat
    51781 aggctccatt ttatactcaa tgaaaatcaa agagcaaact aggaagctat ctgcaacctc
    51841 aaaacactgt tttgaggttg cagatagagc tgacgtggtt tgaagagatt ttcgaagagt
    51901 attaagatta tttcttctag ttcagggtgt tcatacacca aactccctac tactcgatct
    51961 aggatatcta ccgtgtccca ccaggatttt gtcacgacta actgctctag cttaggcaaa
    52021 tcggtttcct ttagataaga ctgcattgct ttcaaatagt tagcagccac atattggtat
    52081 tttctaggct ccttttccca gcaagtgtct gcaaaatccc aatcgataat ctttgttttt
    52141 ttcgcttctg gtaaatattt ttatagagtt tatttctttc aggcaccgca atacctagaa
    52201 aagaaaattg atggcgcata taggcttcca tgggccttgc ttttttagag tcttttgctg
    52261 cttctagctc ctcaagtaaa tctgctaaac tcatctaaaa ctcctcttgc cccaccaaat
    52321 ggtgctgaaa ggcatagaca gccgcctggg tacgatcgct gacttcaagt ttggcaagaa
    52381 tattggacac gtgggtcttg accgtcttga gagagataaa gaggtcatct gcgatgcgct
    52441 gattttcgta gcccttggcg atgagttgga gaacatctcg ctcacgcgca gtcaattctt
    52501 catgaagttc catatgattg cggtggtatt caaccttctt gctaacctct tgctcaatgg
    52561 ccagctcgcc agcagctacc ttactgacgg catgaagcaa ttcatctgca ctagaagtct
    52621 tgagcatata gcctttggca ccagcatcta agactggcat gattttttca ttgtccaaat
    52681 aagaggtcac aatcaaaatc ttggcttcag gccattcttt aaggattgct aaggtcgcgt
    52741 caatcccatt catctcaggc atgacaatat ccatgacaat gacatctgga cgcagttcca
    52801 aggccaagtc aatcccttga gacccgttgg acgcctcacc cacaacttct acatcgtctt
    52861 ggaggtcaaa gtagcttttc aagcccaaac ggaccatttc atggtcatct actagtaaaa
    52921 ttttcatctt tactccttta tcattcctta tctaacaggg gaatacggat atcaactgcc
    52981 agcccttgct tgggagctgt taataactga accgtccctg ccatatcttc aacccgctcc
    53041 ttgatatttc gcagtccata actcaagtcg tctaagcttc ctaaccggaa accaatccca
    53101 ttgtccacca ccttcagttg caattcaaca tctgtctgat agaggtagac atctaggcaa
    53161 gatgcctggg catggcggag ggtattgcta atcaactctt gcaggatacg gaagatatgc
    53221 tcctcgattt tcttaggcaa tttcgtcata ttctgcttga gactaaccct aagatcactc
    53281 ttgtcctcaa gctcttttaa gagaatctga atcccttcta tcaagctctt ctgctccagt
    53341 tcaactggtc gcaaatgcaa gagcaaaacc cgcaaatcct tctgggcagt ttctaaaata
    53401 gctgtgacac tctgcaactg ggtctgcatc ttttctctat ccaatttcaa agcctgctga
    53461 ctgatacccg ataaaataat gtgggccgca aacaactcct gactgactgt atcgtgcaaa
    53521 tcccgagcaa ttcgcttccg ttccttctcg ataatttcct cttcctgatc aaggctatga
    53581 ttttcagctt tttgaagagc ctctgtcaaa aggttaagtt tacctgataa ggacttgaaa
    53641 ctggcatcca aatctggatc tacaacctga actacttctt gccctgccaa taaacgcttg
    53701 agattagcct gcatttttct tagagaaagc tcttcgatcc ctcgccaaaa cagggctaag
    53761 agacaggtca tggacatgct gaaaaccaac aataaaaaga caaatttttc tgttttttcg
    53821 acatcgtaca aaaagataga ccagtcaaaa tcaagtattt ccagcaagct gtgggagaaa
    53881 aaaaagacaa ataggaagga ggtgagagca ataatgacat aggcttgttt tttcatcctc
    53941 taaccacctc cacatcacca atcatagtgg tcaagaaaat cttgacactc ttgttactct
    54001 tgagatagtc ttttgtttct tgatgatagt gttcattgcg gagggctcgc ttgggctggt
    54061 tgaaaaaagt caaatcccca tagagacagt taacgctgag actgacttcc acatctacag
    54121 gtacgatgat tttggtcgtt cctaccatct ttctgaggat aatgacattg tcatgattgg
    54181 ttaagatgac tctctccaga tgaatagtgt ccttgcccat gaagcgaaag agattgatat
    54241 catcaaattg gcaagtctgg tagcttgaaa aatgatgaag atttccaaac caacgatttt
    54301 tctccttctt aaccgtcaca acctcttcaa aaaccaaatt ggtctgctct ttttcctggt
    54361 tcatcatcgg gtaaagaaga aagagactat agataaccgc aacaaaaata gctagaatca
    54421 caaaaggatt gagcataacg atgaaaaaga agagaatggt tgccgctact aaaagaagat
    54481 tattcccctc tttaccagta tagtagcgaa tcaaaagcaa aaagaggaat agtatcagca
    54541 gaaaacgcga aaaatgctct gataccatca aaatcagagc tcctgtcaga agacaggctt
    54601 cgataaataa aaagatttta aattttctca taggttcatc ctctcccttc tattttatca
    54661 caattcaaaa aagtcacctc agtctgagga tggaaaaaaa ggcgctggtt acgccttttt
    54721 catctgatcc tttgcttctt ttaattttcc ataaagaaga tagtctactt tttgtagatc
    54781 tgctatggtg gcacagttaa gggaacacat aatcaagcgt agatctgctt tccagccttg
    54841 gacaatgcca atcacttctt caactgtgta ggtttcaacc aattccagaa cggttcgtga
    54901 caatcccaca gccttagcac caaaaaccaa gcacttaatc atatccagcg gattccgaac
    54961 ccctccacta accaagagtt cgaccttatc tttccattct tgggcattga gaagggcctg
    55021 catggtagac tgaccccatt gattgaggta atcacgctgg ccactacgac ggttttcgat
    55081 ataggcaaag ctggtgccac cacgacccga taggtccact gtacgaacac cgaattcata
    55141 ggctctttcg attgtcttgg catccattcc aaagcccact tccttgagga caataggaac
    55201 gggaatttgc ttgctataat ctgctagatg cgattgccag cttctaaact tcctttctcc
    55261 ctcgggcatg agtaattcct gcatgacatt gacatgcact tgcaatagaa caggattcat
    55321 ctcttctaca gtctgaagtc ctaactcgac aggtttgtcc aatccaatat tggttccaag
    55381 gaggagattg ggatgactag acttgacaga aaaagaatca tccgttggat ttttgagggc
    55441 tgcgctataa gaacccgtta caaataaaat accacaggat tccgccacct gagccagctt
    55501 ttgattgatt tctcttccct tattacttcc accagtcatg gcattgatat aaaaaggaaa
    55561 gtcccacttt cgaccagcaa actctgtcga aagatcgatt tcatccagat tgtaaagagg
    55621 caaggaagaa tgaatcagct ccacctcatc aaagctatta taggaacttt tctgctcaag
    55681 ggcatagagg atatgctcgt ccttacgatt tgtcgtcatg tcctatcctt tcttgatata
    55741 agagctcaat ccccagatcg gcccaacgat tttttaaggt tttggttgat tgcgcatcaa
    55801 aactcagggc gatgccacag tcaccaccac cagcaccact actcttggca acggcctgca
    55861 aatcttgact ggcttctttc aactgtctaa gcaaaggcgt gtaaatatct gtactcaagc
    55921 cttctaaaag cttgctggct acttctactt gctcgataat cttttctgat ttcccctgtt
    55981 ccaaggcttc gaccaaagaa accaccgttt cttttgagga acttaaaaaa ttttgattga
    56041 tattttgctt gatttgctgg accatgtgac tcgatacagc cacttccttg gtccatccca
    56101 ctaagaaatc acattctaaa gttggtttca cttgtgaaat tgaaaagccc caatcacgct
    56161 ccagaactgt cgccaagttt tcttcttcta accaagcagc caccttctgg cgatcaaatg
    56221 actggtagag aaccaaatcc tctgccacaa tacaggcaag gtcgcccatg gaaccattgt
    56281 ctcctcgctt gagcaagaca gcgctagtca gcttgaacaa gagctcctga tcaacagaaa
    56341 catcatacag agccagtaaa gccttgacaa ccaagacaac gacgctgcca ctagaaccta
    56401 gaccaaactt tttcccttct cgttccattt tgccacagat ttctagggaa aaagatctta
    56461 aattctgacc gcgaacagcg aggaagtctc ccatcaaagc aatcgtttct tgaatcaagc
    56521 tgtagtcagg attgggcgtt aagtccactg cgaaatcaaa catatctgaa tagatacggt
    56581 agctgtcaga aaaagcaatc tcagccctca tatagatggg aatatccttt atcaaagcta
    56641 actgccctgg ctctaaaata gcatattcac ctgcccaata gagttttccg caagttttaa
    56701 cagcaatcat cttgactcaa atcctttgtt tttgacacaa tcaagcgata acgctgaccg
    56761 aaaatttctg acaaatgctc caagtctttc tcctgacaaa agaccttaac attgggacca
    56821 gcatccatgg taaagtagca ggcctctcct ttctcacgaa gctggcgaac aaagtccata
    56881 gcctcataag aggcatccgt cagataagaa aaggctggac tagcagtctt tgtcgtagca
    56941 tgcatagcca gggcattttt ctccgttaat tctccaatct tggcaaaatc attttccttg
    57001 agataaatca gcatatcctg atagtccttc tcagactgac gaacccagtc gtcaaaagtc
    57061 gtcgaggttt ccacacaaag tttcatcccg tcacggctag agattggttt tttcttgtcc
    57121 tctagcacca acataatcat agctagtttc aagtctgtct ctacagagta aatttctcca
    57181 ctatccttat cccaggctcc tagcggtcca taaaaactcc gagaagaaga gcctgaggca
    57241 aacttggctt cctgcgctaa ctgacttcta tccaatccaa gcttgaaata agcattacaa
    57301 gccttgacca gggcggacaa accactagaa cttgaggaca ggcccgctgc agtaggcata
    57361 ttgttttgag tatcgatacg gacaaagccc tcaccagctg gacgataacg gtcaataatc
    57421 ttactcatct tggcatgctc gacctcattt tgtagctgac cattgatgta aaattcgtca
    57481 gctattacat tggctggtaa aggcgacaag gtcgtctctg tatacatatt ttccaaagtt
    57541 agagaaatac tgctagtagc aggcaccatc tctttttctt ttttctttcc ccaatatttg
    57601 ataatagcaa tatttgcgta ggaacgtact gtcacaggct ctctatccat gtctgaacag
    57661 ctcctttctc ttctaatctt tctgctagtt cttgtgcgtg tgtcaaattg gttaccaagg
    57721 ctatgataca acctcctagc ccaccaccgc tcatcttggc acccagagca ccatggctaa
    57781 gagtcgtttc aaccaaaaag tctgcctcag ggctactgac tccaatttct tttaaatgta
    57841 aatgcgcttg actgaggatt tgtcccagtc cttcagcatc tttttgtgaa atcgcaactt
    57901 ctgcttgctg ggttaattct cccaaggcat gcaaaaacgg tagggcatcc ttgcccttat
    57961 tttgaaccac ttggatggct tcacgagtat gaccataaac acccgtatcg gcaatcacca
    58021 aataggcgga taaatccatc tcaagttctg taaatcctac gttcttgata aagcgaatag
    58081 gttggtcact gagacaggtc ttagcatcca aaccactagg attcatatgg gcaatcatct
    58141 cagctcgatt gaccaagatt tctagtacat catgaggcag atcagcctga tagtagtcaa
    58201 ataccgcacg aatggccgct atgctgatag ccgctgacga acccatcccc cgtttctcag
    58261 ggatagccga gtcaatctca caacgaatgc aggcttctgt gatatccaaa tactccagtg
    58321 aggcataaac cgccatggac aaggtatcct cctcataaag gcgccaagga ctctctgcag
    58381 gaactacctt acaggtcacc tccacctcca aaagaggcag ggaaatggca ggataaccgt
    58441 aaacgaccgc atgttcccct attaaaatta tcttactatg tgcctgaccg acaccaactt
    58501 tttttgtcat tttttccttt tactagacga aaaaacgtct tatttttcat acaagtatta
    58561 attctttcct atctatttta ttatattttc acaaaaaaag cgattgtttc cattcacaat
    58621 cgcttctttc attattgaac ccattcgcca ttatagttga cagaatagcc atctacggtc
    58681 gtattcactg ccaaggcacc tgagctataa gcgtagtacc atctgccatt gacctggaac
    58741 caacctgtcg tcatagaacg acgaaagaaa ctccataccg ttaagtaaag aggaaagtcg
    58801 tgagggatca tgcgccattg acaacctgtt ttagtgacgt acaaagtctc attaacaagt
    58861 actcgtttcg gccatttata ggtgcggtgt ttggagaaat agggttcaat cttagcccat
    58921 tcttgatcgt ttaaatcagt atcatatgct ttgcgtgtca taactctagt ttaacatttt
    58981 ttgtgaatac aagttctaaa taatcgacca cgaaaatttc ttaagtggaa aacgccttat
    59041 gaagtatgct acgggaaact tatgcactta atttgacaat tcaagatgta aaaatatata
    59101 ctatagtaga ttgaaactag aatagtacac ctctacttct aaaacatttt tagaaatcga
    59161 tttgactgtc ctgatcgatt tgtcctgtta ttattttatt ttactataat atttgacaag
    59221 ttatcctaaa agtattatta tgttgttgtg ttatagattg attgaatcta actaaaggat
    59281 cctattcaat tactagaact atcacatact caaggtcagc tcacagatga gcaactattt
    59341 tggttacaat gtctactaaa tttaagtcaa acaaataatt tagtcaaaat taaaaaaata
    59401 gaggaacata aatatgatta caaaacagaa tgtaatagtg ttctacgatt tttactagat
    59461 aaaactgtaa attctgaagg aaggatcact tcttcaacag aatttggaaa tttcgtaagt
    59521 aatttatcat tccaacacgg aatagctgga ctactgtttc ctctaaataa attgtacccc
    59581 ccagaactgg attctaaaat actctctatc atcaagaagg cagtgacaat tagaacgaca
    59641 cacatatgaa tatcaatact cactgctatt tggtgatgca ggctatctat ggttactcct
    59701 acatttattt tctatcagta aaaatcaata ctatctacaa ttagcaaacg tcaccgctaa
    59761 gaaattaata gagaattatg atactctaga agaaatagac tttgcattgg gaaaatctgg
    59821 tgtcctatta tcattaataa aatactatca atttaccaat gacaatactc ttaaaacttt
    59881 catccacaat agtatatggg gaaatttatc attatttcct acaaagagat acagccaaag
    59941 aaagcatttt agactatagc tttgctcatg gatattgtga aattgcatat gctttatttg
    60001 cctattctaa agtcttagaa ccttctatgt tctataatga tctccataca ttccatgctg
    60061 aattaaaaaa attattagaa aaagttactt ctaatactga aaatttagga aatttacaac
    60121 tttcttggtg cgaaggaatt tccggaataa tcttatatct ttgtatgtac gattgtgacg
    60181 gaaacaaaga tattattagt aaatatcaag aatttgtttt taaccatcat ctaaaaatga
    60241 tgacaggata ttgccacgga ataactagct tactacaaac cactgtctac aatcaaaaca
    60301 aattactgat gaaaaaaatc caacaggtaa ttttagcatg ttctgaacga gatgatcacg
    60361 gtttactgat gtttcaagga gatagtggta aagcagattt gtttgacttc ggaataggaa
    60421 gcagggggta tattggtgtc tattaaataa taaattccca ttcgatgtgc aaacataagg
    60481 agaaatgtat gaaattattt tggacaaaca acatatatag acagttgctg ctaaacagct
    60541 gtttttcatc attcggcgac agtattttct acctcgccat tatcaattat gtggctcagt
    60601 acaatttcgc tccgctagcg attttactga tttccatttc agagatggtt cccctactat
    60661 cgcaactctt tctcgggatt ctaggagatt ttcaagaaaa tagagtcaaa cacgcactct
    60721 gtattgccaa aatcaaaatc ctgctctacg ctattttgac agtatttctc gtcttgtcgc
    60781 ccttttcatt agtttcagtc attatgattg tcatcatcaa cctcatctct gacaccttga
    60841 gctacctgtc tgcctacatg atgaacgccc tctacatcag tgtaattaag gatgacctgc
    60901 atgatgccat ggggttcagg cagtctctga tgagggttgt ccacattgtc gccaatctgg
    60961 ctggcgcatt ccttatcaat gttataagta ttcaaactat ttcccttatc aacactctga
    61021 cttttgtcat tgcctttttg ggcctgtatg ttattcgaca taccttgtat gaggttgaaa
    61081 aaaggattga aatgtcacat acagcactga gttttaagaa atattttcaa catcttaaac
    61141 agtcgctggc tgtgctcctg aggttaaaag ataccgtcat actactgttt ctgacgacca
    61201 gtatgattgc catcttggat gtgtcccctc ggctgattgc cctccgcttc atccaacaga
    61261 cactagcaca actgagcatt gggcaactct tcgccctgct ctccatcatc atgtcttgtg
    61321 gagctatcct tggcaatatg accagcagta atctatttaa aaatatccgt ttcacgcacc
    61381 tcttggtttt ctgtgagatt tccctattgg ctctaataac tagtatcctt tgtcaagcct
    61441 atatcgtaat tttcgtgacc agtttcatca gttctacgat tatcggcatt ctcagccctc
    61501 gcctacaagc agctgtcttt gcccatatcc ccagtgacaa gatggggacg gtgggctctg
    61561 ctctgagcac agtagacatt ctcgccccgt ccctgctctc cctattagcc ctatccatag
    61621 catcgggcgt ttcggtgcag ttagcattga tatttttgta tcttattttg attgctctta
    61681 tcttttgtca atggttagtc aagttcaaca ctcataacta acgaaaaagc atgtgtagat
    61741 ttcacatgct tttaatctcc ccaatcgtca ggtcaagtac aacaaagtca cttctttgat
    61801 taagcgagtg ttctaatata attataagcg ccctgtcatt accgaaccca ttcgccatta
    61861 tagttgacag aatagccatc tacggtcgta ttcactgcca aagcacctga gctataagca
    61921 tagtaccagt tgccattgac ctggaaccaa cctgtcttca tgtctccatt acctgcattt
    61981 aggtagtacc aagttgaacc atcttgatac caaccagttg ccatagctcc tgatgaacgg
    62041 agatagtacc atttgttccc aaggttttgc caacctgttt tcatatcgcc atttggctgg
    62101 tctaaataat accaagtggt accttcctga taccagccag tggccattgc tcctgaggaa
    62161 cggaggtagt accatggagc aggtttgctt ctatcaatgt caatagtaac atgaaaatct
    62221 tgagtccaac ccttagcaac ttcaccaatt ggtagtggat tgtaagaatc atcaccaaaa
    62281 tttcctagct tatttaaata ataccaattg ccgtcttcat aaatccagcc tgtctttaca
    62341 gcataattat tatcaaaata ataagttctc ttttcagcag atagaggata ttgttcaccg
    62401 tatacttttc ctgtattttc agacgttttt gcctctagta cttgtttatc tgtttgttct
    62461 agcaaagcac catcttgtcc aaaatagtac cacttctcct gaaggccaat ttcgaagact
    62521 ggacgattat caaataaatc atcacgataa cctgttccag gaatttctaa gtattgccaa
    62581 ccgacaacca tctctccaat atcaccaaaa tagtaagttt taccgtctat tttatgccaa
    62641 tagattgctt tatgatcatc ttttacataa tattttctat tatccttatc aacaaatcgg
    62701 ccacctgtgg tattcgcaaa taccgtattt gtagctagca aaccaaagaa aaagactgct
    62761 agtagaactt gcatcatttt tttcaaaagt ttcatttata tatcctccta aaacgattgt
    62821 agcaaagaca cgactgcatg tcaatatata aaaactcctc aaaaaagcag ttacacaact
    62881 gcaactgctt ttctattctt gcatggtgta accaacacca cgcacggttt taatgtagct
    62941 tttttgtcct ttaacatcaa gcttgctccg tagatagcgg atatagacat ctacgatatt
    63001 tgtctcggtc gcactttcat acttccaaac actttccaac aattgctcac gagtcaatac
    63061 tttcttgctt cccataagtg tcgccaaaag gtcatactca cggcgggtca gagcaatcat
    63121 ctcttcacca cgataaaccg tgtgatgttc aacatctatc ctaagattgc ggtaggtcct
    63181 tggaactttc atcagactgc agtgttgatc aatgaagtcc cgacctcgga agatcgccga
    63241 aatacgcgct accagatttt cgataaagac tggcttatag atgtatgaaa ctgcaaaacg
    63301 ctgaacaact tccagctctt cttgcaagtc ttcccaatga tctaaaatca tgatgactga
    63361 ggcaggttta gttcgactca atttttctgc aaaatcctga gccatcatat ctcccagatt
    63421 aacgttcaat aaaatcaaat catagtctgt ctgaagagcc atggaaaggg ctttttgccc
    63481 ctcctctacc agatcaaccc gatactgctc tttctggagt tccaaactta aaaaatgagc
    63541 tagatttcgt tctttctcaa gtaataaaat ccgtttcccc atggctgacc tacttatttt
    63601 tcgtcatacc aagagtagtg gaaggttcct tctttgtctt tacgttggta agtgtgagca
    63661 ccaaagtagt cacgttgtgc ttggatcaag ttagccggaa ggtcagctga acggtagcta
    63721 tcaaagtaag taatagctgc tgagaaagtt ggcactggca caccagcttg aaccgcaaga
    63781 gctacgatat cacgcactgc ttgttgatac ttagcagtaa catccaagaa gtactcgtcc
    63841 aaaagaaggt tggcaagatc tgcatcgcgg ttgtaagcat ctgtaatctt ttgcaagaaa
    63901 cgagaacgga tgatacagcc atcacgccag atagatgcga tatctgcaaa tggcaagttc
    63961 cagttgtttt ctttagaggc tacacgcaat tgagcaaatc cttgtgcgta tgaaatgatt
    64021 tttgagaagt aaagggcttg acggattttt tcaatcaatt cagccttgtc tccttcaaag
    64081 ttgaaggcag ctggttttgg aagcaccttg ctagcatgta cacgttcttc tttgtaagtt
    64141 gaaatgtagc gtgcaaacac tgactcagta atcagtgaca atggtacacc aaggtcaaga
    64201 gatgattggc tcgtccattt accagttccc ttgttacctg cagcatcaag gatgtagtct
    64261 acgattggtc catcttggtc ttcatcgtct ttacggctca agatatcagc tgtgatttcg
    64321 atcaagtagc tatccaattc acccttgttc cactcagtaa agatttcagc catatcctct
    64381 gcagaaaggc ctagcaagtg ttgcatcaag tcatagcttt ctgcgatcaa ttgcatatca
    64441 ccgtactcga taccgttgtg aaccattttc acatagtgac cagctccatc aggaccgata
    64501 taagtcacac atggtttgcc atcttctggt gctttagctg agatttcttc aagaacatcc
    64561 gaaaccaatt cgtaggcctc tttttgtcca ccaggcatga tagaaggacc ttcaagggca
    64621 cctttttcac caccagaaac tccagtaccg ataaagttga tacctgagtt tgccaattct
    64681 tcattacgac ggatggtatc tttgtagaaa gtgtttcctc cgtcaatcaa gatatcacct
    64741 ttgtcaaggt gtggaagaag ggcttggata gtagcatctg taccaggtcc agcttgaacc
    64801 atcagcatga tacgacgagg tttttcgatt gagtttacaa aactttcaac gtcatagctt
    64861 ggtacaaaat tcttttcagg atggcaagca atcacatctt ccgttttttc tttactacgg
    64921 ttgtagatag caactgtgta accacgtgat tcaatattaa gggcaaggtt acgacccatt
    64981 acggccatac ctacgacacc aaagttagct tttgtcattt gacactcctc ttgtttcatt
    65041 tgttttattt tatcattttt acttttgaaa agaaagaatt ttgtaacgac tgcttagtag
    65101 tccaagtcat ccgcatgacc agcgccattt ccgacaaagt agcctgtctt acagttaagg
    65161 gtaaagagat aggttccatc tggcttatag aggttgtaat aaccattgcc gttaacgata
    65221 ttgacacgtt caaggatata ttggtctcca gtgatatagc cacgtgaacg tgaagttgct
    65281 agaatttgtt ccaaaacacc atccgcaaaa tcccaggcag agttattact atcatcaata
    65341 gcagactgat tataggcaac acgactggca cttctttgaa cagcaacccc tgcactcgag
    65401 agacccatat tgacttcact gcgactactt ctagtctcat ttgaactact tggttttggc
    65461 tcactagtcg tatttgaact tgcttgactt gaacttgagc tgctagtttg acttgaactt
    65521 gagctagaag tacttgtttg ctggctctta ccaagactga tagccttatc tagcacttta
    65581 tcaagctccg tatttccagt tttaatatcc gtaaatttag catccgattt ggctttggca
    65641 ttggtatcca acacaccatc cacaatagct ggtttttcaa attgtgcatt gacatcttga
    65701 atggccttga tttgcgttac tagactatca tatttagatt tggcaagcgt atgttcacga
    65761 ctaccttcca gcttatcaag taaagtcttg agttgactca gtttatcaaa ctggctattt
    65821 ttcaaagccg ttttattgct atttgtgtaa aaggcgtcat aaagtgtatt aaaatcatcc
    65881 acatccgatt gattggctgt agttgattga gaagtttcaa tttccttggt cgaacgagcc
    65941 acttgacgat agacataata agcactgaca caaataatta ctgatacaag cgccaaaacg
    66001 gtcaaaataa agcctttttt acttttcttt tctgagtcct cttgatccag acgagaaagg
    66061 ttttgctcct cttccacttc ttcagtcatt tctgttggat tttcatcatc tagcaataga
    66121 ggatccaagc cttgctcctc atcgtccaga ggcaagggta gtaagatgtc ttcagaagat
    66181 ggaacttcct ccgaagaagc ttctgacttc tctacagcct cacgcatctc ttgaattaaa
    66241 tcatccagac tctgagtttc aacgaactcc tcttttttgt attgacgagt cgcaaactta
    66301 tccgcctcaa tttcatctct gtgttgctta acatacttgt ccaaaatgga atcctcaggc
    66361 aagactcctg cttccacttc ttcattttta cgaatagctt gaccaactgt tagctctttt
    66421 gcttcatcaa aatcaaattg cggttcttga ccttcttttt tatgacgatt tcgtcttttt
    66481 ttactcatga gtatcccttt ctattttacc tcttgccgtt tgacacgctg accaaaatat
    66541 tgatatagat ccactttcaa ggttccgttg tataacttac gcttcttatc tgcttggcta
    66601 ccatacttgc tttcaaaagc ttcatcacta gtcaggataa atttgctcca agttttcagc
    66661 ggtgcaaata cttgccccat ctcagcatag agcttggtca ctcctgcatc atctgacaaa
    66721 cgttcaccat aaggcggatt ggaaatgatt actccattga ttttatcgga acgtaaatcc
    66781 tgcacgcgca tctgcttaaa agtaatatct cctgcaacac cagctgcctg agcattggcc
    66841 ttagcaattt ccaccatgcg agcatcaata tcacagccca tgatatccaa ctcaagctta
    66901 cgttctactt ttttagccgc ttctgtgcgc acttcttgaa tcaagcgatc gctgatccag
    66961 ttccattcct caaatgcaaa agagcgacga agacctggcg ccatctttct agcaatcata
    67021 actgcctcaa tacagaaagt ccccgaacca caggtcggat caatcaaagg cttgtctgga
    67081 taccagttag aaagttgtaa aatggctgct gccatatttt ccttgatagg agcgccacct
    67141 ttttcggtac gataaccacg tttaaagagg ctagacccgg tcgtatcaat catgacagtt
    67201 gccacatctt tgagaataga gacctcaatc ttaaactctg ggccattctc catcagagga
    67261 accccttctg ggcgagcata gtgtttctgc aatttcttga caacagcttt cttagaaata
    67321 gcctgaacac tgggctcatt gtgaagtttg gacttaacac atttagcttt tgaaatcggt
    67381 aaccgagctc caagtggtaa ataattttcc caatctaaag cgaaaactcc ctgaaatagc
    67441 tcttcaaaag tcttagctgg gaacgttcct acgataattt tgatacgatc tgctgcccga
    67501 agccaaaggt tggtttcgat aatagctctc acgtctcctt gaaaacgaac acgtccattt
    67561 tcaacctgac aatcgtagcc caactctcgc acttcacgac caacgacagc ctcaagccct
    67621 gctgccacag ttgcaattaa attaaattct tttttcatgt tacagtcttg caagaccttt
    67681 ctatatgtcc actttaaaaa atgaggttga gaaaatttct cagccccaac ctatatgcaa
    67741 ttttctataa gccatgtttt gttccagaag tgactaggac cacttccttc gataatcatc
    67801 tgtctaccac taacagctct agctgatagc agtcagaata ctacgttcgt ttccgcatac
    67861 tccatgcccc gaccaaaatt tgggttgcta gcttgagggg tttaccgcgt tccactctct
    67921 ctgtttccag aaagactccg tcactgtggc actttcaagc ctactctggc ctatccaagg
    67981 acttagccat ttcaactgcc gtaacgattt ctcgtcccta ggcttatggt ttcgcctagc
    68041 acaaacacta caggcatcac agcctgtgct agcatggact ttcctcatga gaagcaattc
    68101 tcctcacgcg attatccaaa aattgcacat ctcaaataac tacttaaaaa tctgagttat
    68161 ctaaaatttg tttaccaaaa acttcttttt ccaatctatt caggcgtttc aaaatatcaa
    68221 aattcgtcat agaacttgta attgccgctt caaggggttc tgcttgaact ggtgaaggtt
    68281 tcggtttacg agttaattct tccttcaaat ccgcaatttc ctgacgaagt gacttgacca
    68341 aggcagcata ggtttcatag tccttgatga catcgtctaa aaactcgtca acttctactt
    68401 tattatagcc acggacttca cgtccaaact cttgttcaaa aatatctttc gctgaaaaaa
    68461 taatacttgc catgtctctc tccattctcg cttgctagta ttattatata aaaaaaggca
    68521 aacaaaaatc aaggtcaaag cttcaatttt cggaaaaatt ttcagcaagt tcatttaatt
    68581 gatcaaatgt taatctcttt ataaaatagt cctcttgatt tttcatcttt tggtaaaaat
    68641 aagctagttt tgtttcattt tcttcatcat aaaaaagata ggaactagtc gtgttttcca
    68701 gcaaaaactg ctggtaatct cttaactgcc ccttgtgttc atagcgagga taagcatatt
    68761 tgacaaagtc tacctgctta aagcgactca gtttcatctg attgccttca ttccaatttt
    68821 ccccatgggt ttcaaaagca aaaatggtgg ccaactggaa accgtattcg gttttcatct
    68881 cctgagcaac ctctaaaacc caatattcaa aacccaaact ccctgtaaac acaagccaag
    68941 acaccccatc tgctgccata gcctctaaat ctttacgtat cgctttttta atcaatttaa
    69001 gacgaggatc cttatcagaa aataaaccca aatcaaaagc agaatagcct aaaaccaaag
    69061 ctgtagccat ttttaaccct ttctgtgcaa attatggtat aataaagtga tgttattgta
    69121 ccaataagga gaattatgat caactatcca cataaagttt catcacaaaa aagacaaaca
    69181 tctctttctc aacccaaaaa tttcgcaaat cgaggaatgt cttttgaaaa gatgatcaat
    69241 gctaccaacg actactattt gtctcagggc ttggctgtta tacataagaa accaactcct
    69301 attcaaatcg tacaagtgga ctatccacaa cgaagtcgtg ccaagattgt tgaagcctat
    69361 tttcgacaag cttcaacgac ggactattct ggcgtttata atggatatta catcgacttt
    69421 gaagtcaagg aaacaaaaca aaaacgtgcg attccgatga aaaattttca tccacatcag
    69481 attcagcata tggaacaagt ccttgcccaa caaggaatct gctttgtcct tcttcacttt
    69541 tcttctcagc aagaaaccta cttattgccg gcattcgatt tgattcgctt ctatcatcaa
    69601 gataagggac aaaaatcaat gccacttgaa tatattcgag aatatggata tgaaatcaag
    69661 gctggtgcct tccctcaaat tccttatctc aatgttatca aagaacattt attaggtggt
    69721 aaaacaagat gaacaaacca acgattctgc gcctaatcaa gtatctgagc attagcttct
    69781 taagcttggt tatcgcagcc attgtcttag gcggaggagt ttttttctac tacgttagca
    69841 aggctcctag cctatccgag agtaaactag ttgcaacaac ttctagtaaa atctacgaca
    69901 ataaaaatca actcattgct gacttgggtt ctgaacgccg cgtcaatgcc caagctaatg
    69961 atattcccac agatttggtt aaagcaatcg tttctatcga agaccatcgc ttcttcgacc
    70021 acagggggat tgataccatc cgtatcctgg gagctttctt gcgcaatctg caaagcaatt
    70081 ccctccaagg tggatcaact ctcactcaac agttgattaa gttgacttac ttttcaactt
    70141 cgacttccga ccagactatt tctcgtaagg ctcaggaagc ttggttagcg attcagttag
    70201 aacaaaaagc aaccaagcaa gaaatcttga cctactatat aaataaggtc tacatgtcta
    70261 atgggaacta tggaatgcag acagcagctc aaaactacta tggtaaagac ctcaataatt
    70321 taagtttacc tcagttagcc ttgctggctg gaatgcctca ggcaccaaac caatatgacc
    70381 cctattcaca tccagaagca gcccaagacc gccgaaactt ggtcttatct gaaatgaaaa
    70441 atcaaggcta catctctgct gaacagtatg agaaagcagt caatacacca attactgatg
    70501 gactacaaag tctcaaatca gcaagtaatt accctgctta catggataat tacctcaagg
    70561 aagtcatcaa tcaagttgaa gaagaaacag gctataacct gctcacaact gggatggatg
    70621 tctacacaaa tgtagaccaa gaagctcaaa aacatctgtg ggatatttac aatacagacg
    70681 aatacgttgc ctatccagac gatgaattgc aagtcgcttc taccattgtt gatgtttcta
    70741 acggtaaagt cattgcccag ctaggagcac gccatcagtc aagtaatgtt tccttcggaa
    70801 ttaaccaagc agtagaaaca aaccgcgact ggggatcaac tatgaaaccg atcacagact
    70861 atgctcctgc cttggagtac ggtatctacg attcaactgc tactatcgtt cacgatgagc
    70921 cctataacta ccctgggaca aatacccctg tttataactg ggataggggc tactttggca
    70981 acatcacctt gcaatacgcc ctgcaacaat cgcgaaacgt cccagccgtg gaaactctaa
    71041 acaaggtcgg actcaaccgc gccaagactt tcctaaatgg tctcggaatc gactacccaa
    71101 gtattcacta ctcaaatgcc atttcaagta acacaaccga atcagacaaa aaatatggag
    71161 caagtagtga aaagatggct gctgcttacg ctgcctttgc aaatggtgga acttactata
    71221 aaccaatgta tatccataaa gtcgtcttta gtgatgggag tgaaaaagag ttctctaatg
    71281 tcggaactcg tgccatgaag gaaacgacag cctatatgat gaccgacatg atgaaaacag
    71341 tcttgactta tggaactgga cgaaatgcct atcttgcttg gctccctcag gctggtaaaa
    71401 caggaacctc taactataca gacgaggaaa ttgaaaacca catcaagacc tctcaatttg
    71461 tagcacctga tgaactattt gctggctata cgcgtaaata ttcaatggct gtatggacag
    71521 gctattctaa ccgtctgaca ccacttgtag gcaatggcct tacggtcgct gccaaagttt
    71581 accgctctat gatgacctac ctgtctgaag gaagcaatcc agaggattgg aatataccag
    71641 aggggctcta cagaaatgga gaattcgtat ttaaaaatgg tgctcgttct acgtggagct
    71701 cacctgctcc acaacaaccc ccatcaactg aaagttcaag ctcatcatca gatagttcaa
    71761 cttcacagtc tagctcaacc actccaagca caaataatag tacgactacc aatcctaaca
    71821 ataatacgca acaatcaaat acaacccctg atcaacaaaa tcagaatcct caaccagcac
    71881 aaccataaca tttatcaccc agatttttct gggtgatttt gtcatagcac tattgtattt
    71941 atgtgcttaa caaaagcctg agaagatgtc ttctcaggct ttttctgtac tataaacctc
    72001 attggaaaag atagagactg cttccgtaat cttgttaaga ggttttacta aatactagtc
    72061 tttcttcgtt tttactacaa agagagcaga tagggctaga ctaacacttg ctaggaagag
    72121 ggctgtgtca gattgactct cacctgttgc tggcaagatt tgttcaggtg tttctggtct
    72181 agctatttct tgcttataat ctggcgtctt accttgatcg gaaactaaag agctaagttt
    72241 aaactcaggt ttctctacag ttggagcatc tggtttcgta gcagaatctt ttaattggtt
    72301 gagataatct gcaaagtatt ccaccattct ttccgtcaag agatggttat ccgtcgcata
    72361 tttcatgctt gcctgaacgc tagctacttc ctcttgattg tctttgtctg ttaatttctg
    72421 agctttagcc aaagctgctt catagcctga caagtcaaga ggtgtttctg ctacctgtgg
    72481 acgagtaaag atgagttccg ctgcagattg gtatttatcg ccaccatctc cgtaagtctt
    72541 agtacctgta aggacaattt tcttagcctt aattgtctta ccaaaatcaa tgtcttttgg
    72601 cttattgtta tctggccaat cagttgcagt aaaggtatgc tccttgccag actcatctgt
    72661 cacaacaagt ttcacatcac gcaagttacc atttgaacct gatccacgtg gaacgtaacg
    72721 aagtccagtg atttcagttg gttctttcaa gaccatggtt gcaggcttgc ctacatctcc
    72781 accattccaa gatgtatgcc ataaactaga taagtttcca tcaaaggcat ttgcaagacc
    72841 ttcttgagcc tgagcaggag ctgtaagact tgcaaagtca tctgctacca aagccgtttt
    72901 cttctgaacc aaagcattct tcaaggcttc aatcttggca atctctgcac gcgcttcttc
    72961 cacactgata tcatcatcgg cctgactaag gttaaagacc gcctctttca aagcatccat
    73021 agactctttg gtgtagttag tcatggcaac cgttggcaag tagttcttca gagcattttc
    73081 tgtcaacatc ttacctgtta gggtaatttc ttcgatttga agattatcca tcatgaagtc
    73141 gttataacca cggaagttgg catttccacc agaatcacca cgagtattac ttgcattgcc
    73201 agttgagtag atacctaccc aagtatcccc tgtttctgca cctgtcacaa ggaaggttgc
    73261 cttcttggct ttcttagaat ctgtccaagt atttggcaat tcatgcattt ccaagttgct
    73321 tgcttgagtg ccacgacgac ctgactggaa ttctccctta ccgactacaa aagcataggt
    73381 attgtctgaa cctgcttcgt attcaaaggt tacacggtag gtcttacctg cttcaaaacg
    73441 gaagttttgt gggatggttt ggtaaaccaa gttacgacgg ctcactagtc catttgtctt
    73501 gagtgaccaa tttccttcga taacatcatc gactttctta ccgttccaac cacgttgtgt
    73561 atatggatcg tgtttttcag acaagtgagt gcggttgtct tcgacacctt cgacaccacc
    73621 cactacaaat gggaagattc cttgagctac attttcaaag tcttgcttga aggttccttg
    73681 acctgtatcg tgcttgtctc catacatgct tgaattgttt tcaaaggtac gaatttcatc
    73741 aaagtaagtt gcttgatcac cagcttcacg actcaatgtc agagtaacat ttgagacgtc
    73801 cgatcctgtt gtaaagaagg cgtacatgtt ttggaagtaa cttgtatcgt taactgtagc
    73861 attgttacga cgtgtattgt gggcgtaggc ttttacatag ttgagggcga gagacttatt
    73921 ggtataagta gtcacttctt tttcaccagt attcacagtg atacttgcct tggcattact
    73981 acggttatcg acaccgacat aaacggcata cttggtattt ggtttcaagc cagttaattt
    74041 ctgagtgaga ctaacttttt ctttgtttcc ttgaatacga agcatatcgt ttgccccttg
    74101 agacttgaca atttctgcct tagaagcatc gcctgaaatg gtccaatgtt tcaaggtacc
    74161 actgttaaat ccttggtcat agatgtgcat gccttcactc catgacattt caggattggt
    74221 ttgttttgaa cgatagagaa cgtatggttg atttgctaga agatctaggg taattttacc
    74281 atcttttaca gttagttctt gctcttctgt cttaccttgg tcagttagct tgtaaaggta
    74341 aaccttgctc tttgcccaat cgcttggaag ggtccaagtt gttgcaccgg cctgcgtatt
    74401 gaagtagtac atcttttcct tatcagtaga aagtttctta ccatttgcat cccagttcca
    74461 aggagtcaag taagctgaac catcttggat gacacgtccg ttgagagtta ctgtacgttc
    74521 gcgatattgt ggactattga catcatttga cttacgagtt acaactactt tattattgtc
    74581 agcatctacc aattccactc gcatttctgg agtccattta taggtactac cgttatcggt
    74641 catagtcacc ggtgtaccat tttcccattt acttacagtg aagtgttgga agtacttagt
    74701 catgacgtca tgggcaaata agttggttac atagccattg tagtcacttc ttccctgcca
    74761 gccttcaaag tctttcatgc tgtagccacc tagcagtgga tagttggctg caccaccata
    74821 acttctgtag tccccaaccc aagcatcttt ttggtggtta cggataaagc gggtgatggc
    74881 actgttgata cctttattgg tgtagccacc gtaggtcaag tcagctgccc agtgatggaa
    74941 ggtagagtcg tactcaccac catggcccca ctcgatcgca aagcgccagc cttgtttgtt
    75001 aatttcttta gcaagaacgt gggtagccca ggcaccgtta tcacctgatt gaccattacc
    75061 ccaaacgtcc acatagataa agtcgagacc gtcaccaagt tttttcttca aatcttccca
    75121 acgtgccaaa cgaccatgag ctaggtcata ggcagcatca atgttgatac cttgatctag
    75181 ccagttccaa ccatagctat agcttccatc tggattctta cggagaattt tttcattgaa
    75241 gtatttagac tcaggataag tttctgaagc gttaacgtgg atacctagat gagctccata
    75301 tttcttagcc ttctcaatta gggtcttgaa gtcttcgaca ccaccgatac gcttaccaat
    75361 atcagcatag ttcaagtgac cagagtcatg gccttcgcta ccatatcctt taaggaaaac
    75421 accttgccca agaccatctg tgtggagatt gattttcttg ataccatcca aggtcataag
    75481 gaatggattt tgtgcttgag aaccaaagtt catcgcgata cggtaagctg tgatatcctt
    75541 aactttttcc caaccttgag gattgttcat aatgctacga taagcaatgg caccatcttg
    75601 ccaatcgact ttcttgtctg cattggcatc ttcagtgata acaaccttag cacttggaag
    75661 ttccttcgtg tattctggga aaacaatgcc cttataagct ttttcccatt gccattcaga
    75721 gctgtggatt cctacatagt tggcatttcc gactgtttct ttataagctg tcaaacgagt
    75781 ccagtcattc gaaccaccac catagctatt ttgagagtta ctccaaacac cagcagcaag
    75841 cttatctgta gaaacaaatc catacatgta acccttggct aggtctttca ttggattggt
    75901 tacatcgata tgatcatctc cgctgacatg cgtattgttt gacatggttg ccccatcaaa
    75961 cttagcacca gtttgatcac tagaaacaga gactaaagca ttgccgagga aactaataga
    76021 agaaagtagt tttctttcgt catcaatctt ttgacctgga gtgacttgat tgtggttgac
    76081 aatcttggtc acatcaaagt gcaattgatt gtccacaact tgcaagcgta ctgtcatttc
    76141 cgcattgatt aagtgagcat catcgcgaag cttcatcaag tactctgctg ttgtctcatt
    76201 gattttctta taagtgactt caggggtgat tcggtggtta ttgataaaga cttggttgaa
    76261 ctgttgcacc tgtcctggca aagtatgtcc attcaagctg tattccttga cacgagggaa
    76321 ggcttggtca atcactgctt tgaggacctt agactggatc gtatcataag tcaccttgct
    76381 atcgtcaaca accggacctg tttctttctg ggcaggagta tcatccgctt ttacgccttc
    76441 ttgattgtct gttttaacgc tgacaactgt acggtcattg ccataagttc ctgccttgag
    76501 aaggatcttc ttctcatttt taagattatc attgaccgcc ccaggcagtg tcactgtgtc
    76561 aaagagattg acatcgttat tgctggcatt gagttgtcca tctgacttga gagtgataga
    76621 aagacggttt acagaacccg gttctggtgc aactacacgg ttgcctttat accatgtgct
    76681 attacctgga gttttgtatt cccagaacca gccatccttg tcataaccga caaaaacatt
    76741 attattggta tctttaaatt tcaggaagac accaaagcgt gatttgccct tttcagaatc
    76801 ttctttgaag gttaaatcaa cagttgcatt tccattggca tcaacggtca agcccttctt
    76861 ttcaaacagg gctggtttgc ctgcgttatc attttgagca gttgagaata gttggttgta
    76921 gcggacacct ttttcttctc ggatagtgac tgttccctgt tgttcttttt tctctaccgt
    76981 ttgccattca ggagttaccg tcttaggtgt ttcaggttta gcagctgcag gtttatcctc
    77041 ttttggtttt tcttcagccg gtttttcttt ttcaagtgct aatagttctt cttctgtctt
    77101 aggtccatct gtaacttctg gagaaccttg gtcttctccc accttaggcg cttcaaaatc
    77161 acgcccatca ttctcgtttg ctgttgcaag agcagtagct aaatcagctg gtaagttcgc
    77221 cgtggaacca gactgcacgc tatctgcaag aaccggactt gtcccaaaga atgcagctcc
    77281 aatcataaca gaagcaacac ctaatgaaaa tttccgaata ctatatttac aacgtttttc
    77341 aaataatcct ttattcataa acttctaaat cctttcattt tttagcaagc gtttacattt
    77401 cctttttaaa aaatactcct tcctctgaaa gtgcaatgct taaccctgat aaataaaaaa
    77461 taattgtact tttaatataa caaataatag ctgagaaagc aataggaaac aagtaaaatt
    77521 aacaaaaatt ttctctattc tgacaaaaag aggctccaac ttttgggggt cagtacattt
    77581 agaatctttc tttacaaatg taaaaaagga ttcagcattc aagagaaata ctaaatcctt
    77641 tcttatattt tgcaacagtt atttcacatg ttttgcgaga tcttcttgag cctttttatt
    77701 agactcttct ttttctttca tccatttttc ctgagctttt tggtattcat ctacagtgac
    77761 tgccttgtct tgaagttcca agtatttata caagactggt tcacttgtac ctttatttcc
    77821 tgacaatgca aatggtattg taaatggtac catcttagac aagattgggc gccctgtacg
    77881 agatgtagtt ggaataatca aagcactatc tgtcaaccaa gcttgggctg cagcgtattt
    77941 atcatagcgt ttagcaacat ctgtagcctc atcaccagcc tcagtgacca atttttcgta
    78001 gtcatataga cctacttttt tagcagctac attatcttcc cctgagtcaa accctaaata
    78061 tgttttagta ctttctccta cagaaggttt gataatatca aggtaggttg atggatcggc
    78121 aaagtctgga ccccaaccga cattatctga taaatcccag tcttcgccag cagcattttc
    78181 agcaaaatat gtaatattgt ttacttcgtc tttttgtagt tgttggatat caataatgac
    78241 attatcagct cctaaagttg cttccaagga ttgtttcata gattggacgc gctgaacttt
    78301 tgtagttgct gtctggtcaa ctggcatatc caaatgaatt gggaaagtca caccttctgc
    78361 ttgtaaggct gatttagctt tagcaaattc agccttggct ttttctggat tgtaaagacc
    78421 atcctgagaa tctgcaagat taacatcctt ccattcatcc ccataagtga ccaatttctc
    78481 tttgaccata tcgccaaagt ttttaccatc tgcttgaaca aatgttggtg gaacaaagat
    78541 attacgtaag attttgcttg ctccagtttg tccattcaac tgagaggcat aggctgtacg
    78601 atcaaaacca aaggcaatag cctgacggaa atccttgttt aagagagcct ttttagtcga
    78661 tgccttttgt tcgtcgctgg tcttagatgt atatttatag gactgacggt caatatttgt
    78721 accaactaga tacgtaatag agccttgttg agtatagaca atattgtcct tcatactctt
    78781 ctcaagctct gcgaaacttg cacttgttgg atagagacga gctgctgtaa ggctaccatc
    78841 tttaaagttt tctgcaggtt tgctggtatc ttgaccatcc cagaatgaca atttaacttt
    78901 gtcaacatgc acattgtcct tatcccagta gttcggattt ttcgcaaatt caacagagga
    78961 ttttgtcaca atggatttca acaaataagg accgttatac aagagactac ttggatccgt
    79021 cgctttggca aaatcatccc cttttgagtt caaaaactct tcattaactg gcgcaagcac
    79081 acccatggtt gtcttagaat tccagaagct ttctggtttg ttcaaagtgt actgaactgt
    79141 ctgatcatcc aaagccttaa ttcctacttg tgagaaatct ttgatttccc cttttacata
    79201 ggcatccaac cctttgattg attcttgaac aaggtaaaga gcatctgatt ttttatcagc
    79261 agcatatttt agtcctgtta caaagtcttg agctttgact gccgcgtatt cttcaccttc
    79321 agaagtatac cattttgcat ccttacggat agtataagtg taagtcaatc catccttgga
    79381 tacagaccaa tcctcagcca tagacggcac aaagttcccg tagcgatcat tttctagcaa
    79441 accatcaacc acgttactgg taatatttgc tgtcgcagcc ttagcagttg tcaaatagtt
    79501 gaggttatca gggtctgtct cataaatgta tgagaatgtc ttctcacctt tagtgcttga
    79561 acctgatcca gagcatgcag ctaaagtagt cgccgccaat aatgtcacgc ccgcaagggc
    79621 aagtagtttt gaacttttca tccttttctc ctttaaaact ttctctccat tatagactct
    79681 tttcagaaag ttgtcaacag aattttcaga atttttgaaa attatttttc aaataacatc
    79741 tttgcaaaaa atatgaatat cgtaaccgcc caataacgaa gtctattgaa aaatctccag
    79801 actagagaac tcacggatag ttcctaatct ggagatttct tatttgcact tttcttgtac
    79861 aactttagtc cacggtaaat aggcctctaa aatctctttg tttacgagag tttcctcgtt
    79921 tggaagacat tctagaagat aggatagata tttctcgcta tttaat
//
LOCUS       DAVBCV010000004        66960 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBCV010000004 DAVBCV010000000
VERSION     DAVBCV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.004"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            114..1031
                     /locus_tag="VQ516_000236"
                     /pseudo
     CDS             114..1031
                     /locus_tag="VQ516_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(1071..2015)
                     /gene="manA"
                     /locus_tag="VQ516_000237"
     CDS             complement(1071..2015)
                     /gene="manA"
                     /locus_tag="VQ516_000237"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2510752.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTILVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2147..2428)
                     /locus_tag="VQ516_000238"
     CDS             complement(2147..2428)
                     /locus_tag="VQ516_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2510753.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            2599..2819
                     /locus_tag="VQ516_000239"
                     /pseudo
     CDS             2599..2819
                     /locus_tag="VQ516_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2936..3283)
                     /locus_tag="VQ516_000240"
     CDS             complement(2936..3283)
                     /locus_tag="VQ516_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2510754.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(3394..5169)
                     /gene="spxB"
                     /locus_tag="VQ516_000241"
     CDS             complement(3394..5169)
                     /gene="spxB"
                     /locus_tag="VQ516_000241"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2510755.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(5376..7619)
                     /locus_tag="VQ516_000242"
     CDS             complement(5376..7619)
                     /locus_tag="VQ516_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2510756.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(7629..8000)
                     /locus_tag="VQ516_000243"
     CDS             complement(7629..8000)
                     /locus_tag="VQ516_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2510757.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            complement(8011..8406)
                     /locus_tag="VQ516_000244"
     CDS             complement(8011..8406)
                     /locus_tag="VQ516_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2510758.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      8590..8688
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8772..9563
                     /gene="thiD"
                     /locus_tag="VQ516_000245"
     CDS             8772..9563
                     /gene="thiD"
                     /locus_tag="VQ516_000245"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2510759.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(9772..10404)
                     /locus_tag="VQ516_000246"
     CDS             complement(9772..10404)
                     /locus_tag="VQ516_000246"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2510760.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(10397..11200)
                     /locus_tag="VQ516_000247"
     CDS             complement(10397..11200)
                     /locus_tag="VQ516_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2510761.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(11200..11724)
                     /gene="thiW"
                     /locus_tag="VQ516_000248"
     CDS             complement(11200..11724)
                     /gene="thiW"
                     /locus_tag="VQ516_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2510762.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            complement(11729..12421)
                     /gene="tenA"
                     /locus_tag="VQ516_000249"
     CDS             complement(11729..12421)
                     /gene="tenA"
                     /locus_tag="VQ516_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2510763.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(12432..13082)
                     /locus_tag="VQ516_000250"
     CDS             complement(12432..13082)
                     /locus_tag="VQ516_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2510764.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAIFPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAYGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(13084..14469)
                     /locus_tag="VQ516_000251"
     CDS             complement(13084..14469)
                     /locus_tag="VQ516_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510765.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(14470..15030)
                     /locus_tag="VQ516_000252"
     CDS             complement(14470..15030)
                     /locus_tag="VQ516_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2510766.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(15292..15380)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15589..16218)
                     /locus_tag="VQ516_000253"
     CDS             complement(15589..16218)
                     /locus_tag="VQ516_000253"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2510767.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(16220..17002)
                     /gene="thiM"
                     /locus_tag="VQ516_000254"
     CDS             complement(16220..17002)
                     /gene="thiM"
                     /locus_tag="VQ516_000254"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2510768.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(17245..17913)
                     /locus_tag="VQ516_000255"
     CDS             complement(17245..17913)
                     /locus_tag="VQ516_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2510769.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(18128..18216)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18470..19606)
                     /gene="lctO"
                     /locus_tag="VQ516_000256"
     CDS             complement(18470..19606)
                     /gene="lctO"
                     /locus_tag="VQ516_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2510770.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(20231..21721)
                     /gene="lysS"
                     /locus_tag="VQ516_000257"
     CDS             complement(20231..21721)
                     /gene="lysS"
                     /locus_tag="VQ516_000257"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2510771.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(21849..21995)
                     /locus_tag="VQ516_000258"
     CDS             complement(21849..21995)
                     /locus_tag="VQ516_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET2510772.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            22221..22889
                     /locus_tag="VQ516_000259"
     CDS             22221..22889
                     /locus_tag="VQ516_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2510773.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            22849..23526
                     /locus_tag="VQ516_000260"
     CDS             22849..23526
                     /locus_tag="VQ516_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2510774.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            23527..24284
                     /locus_tag="VQ516_000261"
                     /pseudo
     CDS             23527..24284
                     /locus_tag="VQ516_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            24296..25157
                     /locus_tag="VQ516_000262"
                     /pseudo
     CDS             24296..25157
                     /locus_tag="VQ516_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(25237..25884)
                     /locus_tag="VQ516_000263"
     CDS             complement(25237..25884)
                     /locus_tag="VQ516_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510775.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(25881..26579)
                     /locus_tag="VQ516_000264"
     CDS             complement(25881..26579)
                     /locus_tag="VQ516_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510776.1"
                     /translation="MGKLSSHMWRLNKIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDVGGDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(26589..27341)
                     /locus_tag="VQ516_000265"
     CDS             complement(26589..27341)
                     /locus_tag="VQ516_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510777.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(27341..28105)
                     /locus_tag="VQ516_000266"
     CDS             complement(27341..28105)
                     /locus_tag="VQ516_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510778.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(28201..28604)
                     /locus_tag="VQ516_000267"
                     /pseudo
     CDS             complement(28201..28604)
                     /locus_tag="VQ516_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28784..29416)
                     /locus_tag="VQ516_000268"
     CDS             complement(28784..29416)
                     /locus_tag="VQ516_000268"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2510779.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYRELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(29450..30151)
                     /gene="pyrF"
                     /locus_tag="VQ516_000269"
     CDS             complement(29450..30151)
                     /gene="pyrF"
                     /locus_tag="VQ516_000269"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2510780.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQNWN"
     gene            30472..30564
                     /locus_tag="VQ516_000270"
                     /pseudo
     CDS             30472..30564
                     /locus_tag="VQ516_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(30886..31599)
                     /locus_tag="VQ516_000271"
     CDS             complement(30886..31599)
                     /locus_tag="VQ516_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2510781.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(31592..32287)
                     /locus_tag="VQ516_000272"
     CDS             complement(31592..32287)
                     /locus_tag="VQ516_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510782.1"
                     /translation="MTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMS
                     LYNNLLFYSKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPA
                     VLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKL
                     VEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLE
                     DIFITLTGGKLNA"
     gene            complement(32627..33959)
                     /locus_tag="VQ516_000273"
                     /pseudo
     CDS             complement(32627..33959)
                     /locus_tag="VQ516_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            complement(33943..34741)
                     /locus_tag="VQ516_000274"
                     /pseudo
     CDS             complement(33943..34741)
                     /locus_tag="VQ516_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            complement(35531..36730)
                     /gene="divIB"
                     /locus_tag="VQ516_000275"
     CDS             complement(35531..36730)
                     /gene="divIB"
                     /locus_tag="VQ516_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2510783.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(36740..37798)
                     /locus_tag="VQ516_000276"
     CDS             complement(36740..37798)
                     /locus_tag="VQ516_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2510784.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VAKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(37800..39152)
                     /locus_tag="VQ516_000277"
     CDS             complement(37800..39152)
                     /locus_tag="VQ516_000277"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2510785.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(39256..39897)
                     /locus_tag="VQ516_000278"
     CDS             complement(39256..39897)
                     /locus_tag="VQ516_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510786.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(39899..41920)
                     /locus_tag="VQ516_000279"
     CDS             complement(39899..41920)
                     /locus_tag="VQ516_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2510787.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(41991..42290)
                     /locus_tag="VQ516_000280"
     CDS             complement(41991..42290)
                     /locus_tag="VQ516_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2510788.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            complement(42656..42910)
                     /locus_tag="VQ516_000281"
     CDS             complement(42656..42910)
                     /locus_tag="VQ516_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2510789.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(42930..44771)
                     /gene="typA"
                     /locus_tag="VQ516_000282"
     CDS             complement(42930..44771)
                     /gene="typA"
                     /locus_tag="VQ516_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2510790.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            44933..45658
                     /locus_tag="VQ516_000283"
     CDS             44933..45658
                     /locus_tag="VQ516_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET2510791.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNELLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(45698..45925)
                     /locus_tag="VQ516_000284"
     CDS             complement(45698..45925)
                     /locus_tag="VQ516_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510792.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(46014..46394)
                     /locus_tag="VQ516_000285"
     CDS             complement(46014..46394)
                     /locus_tag="VQ516_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2510793.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(46394..46663)
                     /locus_tag="VQ516_000286"
     CDS             complement(46394..46663)
                     /locus_tag="VQ516_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2510794.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            46727..47635
                     /locus_tag="VQ516_000287"
     CDS             46727..47635
                     /locus_tag="VQ516_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2510795.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(47689..48444)
                     /locus_tag="VQ516_000288"
     CDS             complement(47689..48444)
                     /locus_tag="VQ516_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2510796.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(48465..49394)
                     /locus_tag="VQ516_000289"
     CDS             complement(48465..49394)
                     /locus_tag="VQ516_000289"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2510797.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(49409..50032)
                     /locus_tag="VQ516_000290"
     CDS             complement(49409..50032)
                     /locus_tag="VQ516_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2510798.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(50025..51263)
                     /gene="hflX"
                     /locus_tag="VQ516_000291"
     CDS             complement(50025..51263)
                     /gene="hflX"
                     /locus_tag="VQ516_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2510799.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(51256..52140)
                     /gene="miaA"
                     /locus_tag="VQ516_000292"
     CDS             complement(51256..52140)
                     /gene="miaA"
                     /locus_tag="VQ516_000292"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2510800.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            52261..52431
                     /locus_tag="VQ516_000293"
     CDS             52261..52431
                     /locus_tag="VQ516_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2510801.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(52479..53318)
                     /locus_tag="VQ516_000294"
     CDS             complement(52479..53318)
                     /locus_tag="VQ516_000294"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2510802.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPIITLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(53415..54374)
                     /locus_tag="VQ516_000295"
     CDS             complement(53415..54374)
                     /locus_tag="VQ516_000295"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2510803.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(54501..55499)
                     /locus_tag="VQ516_000296"
     CDS             complement(54501..55499)
                     /locus_tag="VQ516_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2510804.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDG"
     gene            complement(55549..56166)
                     /locus_tag="VQ516_000297"
     CDS             complement(55549..56166)
                     /locus_tag="VQ516_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2510805.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(56167..57888)
                     /gene="pabB"
                     /locus_tag="VQ516_000298"
     CDS             complement(56167..57888)
                     /gene="pabB"
                     /locus_tag="VQ516_000298"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2510806.1"
                     /translation="MHRKTVIDFRTLGERYTFAQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(57957..63644)
                     /locus_tag="VQ516_000299"
     CDS             complement(57957..63644)
                     /locus_tag="VQ516_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2510807.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEETPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNPKEDRGAEETPKQEESTPDTKAE
                     ETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDP
                     TKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPI
                     EGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAP
                     TITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSI
                     KVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKE
                     VQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLA
                     ITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDL
                     VDAMQKNPNGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFD
                     RVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDK
                     LDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIV
                     GHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKR
                     GDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMG
                     ITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQ
                     GNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVK
                     QVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVN
                     AIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGL
                     TYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFRE
                     VVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYR
                     LYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRESFKSEIN
                     KRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPD
                     YAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETT
                     HVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQW
                     YNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRP
                     MDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTA
                     YVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKEN
                     NGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKT
                     MFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            complement(63666..64229)
                     /locus_tag="VQ516_000300"
     CDS             complement(63666..64229)
                     /locus_tag="VQ516_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2510808.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(64345..66036)
                     /locus_tag="VQ516_000301"
     CDS             complement(64345..66036)
                     /locus_tag="VQ516_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2510809.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(66033..66770)
                     /locus_tag="VQ516_000302"
     CDS             complement(66033..66770)
                     /locus_tag="VQ516_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2510810.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
BASE COUNT        20079 a        14556 c        11839 g        20486 t
ORIGIN      
        1 aaaagtcgtg cgctgaacac caacaagacg cttaaatcgt gcatcagtta gttgtttact
       61 tgcttcatca ttcatagaac tactatgcca tattttgttt cgcaggaagt ctgatcattt
      121 caaatccagc cattgctcta ggagctcaag cgacctttat cctattgaat atcttcattg
      181 tatcacgtgg ggttcaaaaa gggattgaaa gagcttcgaa agtcatgatg cccctgctct
      241 ttatcatctt tgtcgtcatc atcgaacgct ctctcagttt gccaaatgcc atggaagggg
      301 ttctttactt cctcaaacca gatttttcaa aactgactag cgctggtctc ctctatgctc
      361 tgagacaatc tttctttgcc ctctcactag gggttacagc catgttgacc tatgcttctt
      421 acttggacaa gaaaaccaat ctggtccagt caagaatctc catcgtagcc atgaatatct
      481 cggtatccat catggcaggt ctagccattt tccaagctcg gtcccccttc agactgaata
      541 ttgaaggggg accgagcttg gtctttatcg tcttgcctca actctttgac aagatgcctt
      601 ttggaaccat tttctatgtc ctcttcctct ttgcgactgt cacttcttct gtcgttatgc
      661 tggaaatcaa tgtagacaat atcaccaatc aggacaacag caagcgtgct aaatggagtg
      721 ttattttagg aattttgacc tttgtctttg gcattccttc agccctatct tacggtgtca
      781 tggcggatgt tcacattttt ggtaaagcct tctttgacgc tatggacttc ttggtttcca
      841 atctcctcat gccatttgga gctctcttcc tttcactttt tacaggctat atctttaaaa
      901 aggctcttgc aatggaggaa ctccatctcg atgaaagagc atggaaacaa ggactgttcc
      961 aagtctggct cttccttctt cgtttcgtca ttccaatcat catcgtggtc ttcatcgccc
     1021 aatttatgta atcaaaagga cttgagtaat gaactcaggt cctttctttt ttatggatga
     1081 ctaacaatta attctaaatc ttgcccttct agagtccaag attcaacatc acttggtagg
     1141 ataaagtgtc tccctttttg gattggatag tttttcccgt caacagttag ttgaccttga
     1201 ccagccaaga cactcaacaa gctgtagtca gctgtctttt caaagttaac ttttccagta
     1261 atttcccatt tgtaaactgc gaagaaatca ttggatacaa ggatagtgga acgtagatca
     1321 tctgctttga ttgtcactgg acggctattt gcgggttcac caatgttcaa gacatcgata
     1381 gatttttcaa ggtgaagttc acgtaaattg cccttgtcat ccttacggtc aaagtcatag
     1441 acacggtaag tggtatcgct agactgctgg gtttcaagaa tcaagatacc cgccccgata
     1501 gcgtgcatgg taccgcttgg tacatagaag aaatctcctg ctttaactgg cacttttgtc
     1561 aacagcgcat cccagttctt atcctcgatt tgctggcgga gttcttcttt tgacttggca
     1621 ttgtgaccgt agataatctc tgaaccttca tctgctgcaa tgatgtacca acattctgtt
     1681 tttccaagtt cgccttcatg ctcgagtcca taagcatcgt ctgggtgaac ttggacactg
     1741 agccagtcgt tggcatcgag gatcttggtc aaaagtggaa atacaggttc tggacgattg
     1801 ccaaataatt cacggtgttc cgcatacaaa gtagcaagat ctgttccctc gtaacgacca
     1861 ttggcaactt tagaaacacc atttagatga gctgagatag cccagtactc tccgactttt
     1921 tcacttggga tgtcgtagcc aaactcatca cgtagcttgg taccacccca gattttttct
     1981 tgcataactg attgtaaaaa taatggttct gacatcttaa tctcctgtct gatttttctc
     2041 ccctcattat agcaaaaaaa gagttccatt tgaactcttt tttacatctt ataaagcagg
     2101 gagaagattt tataaaaata gtaaacaaat gtgctctact cgatgcttac accattgcta
     2161 taaatgacat gtaccaatag aaggacttct tcttgctacg tttgagagtc ccatttccta
     2221 cattatctcg atctacatag ataaagccat agcgcttatt catttcccct gtgccagctg
     2281 aaaccgaatc gatacagccc catgtcgtat aaccaagcaa gtcaaccccg tctttgtaaa
     2341 tggcatctcg catggccttg atgtgggcct ctaagtaagt aatccgatag tcatctgcta
     2401 cataaccatt ctcatccggt gtatccatag caccgagtcc attttctacg ataatactat
     2461 actaaaatca aaaagcatta tataatagtg atatgaaatc aactaaagaa gaaatccaaa
     2521 ccatcaaaac actttttaaa gactctcgta cagctaaata tcataaatgc cttcaaatca
     2581 ttctatttcg tctgatgggc aaatcttata aagactccga ctaatattgg ttttgcattt
     2641 attcctccat acacaccaga gatgaaccca tcgaacaagt gtggaaagag attcataaac
     2701 gtggatttaa gaataaagcc tttcgaactt tggaagatgt catacaagga ctggagaagg
     2761 aggtgataaa gtccatcgtt aatcggagat ggaatagaat gctttttgaa agcagatgag
     2821 tattatatgc aatttcttta tataaaaaga ccggattgct ccgatctttc aatagttcat
     2881 attctcaatt tctattttaa aaatagctaa ggttaacgtc aaatgactac gcgacctatt
     2941 tcatacgata aaaatcaagc actagaccag caggtccttg aactaataag gactctgttc
     3001 cccaatcggt tacagttggt ccgtgtaaaa cctttatacc aagctcattc aaccgtttgt
     3061 agttctggtc tacatcctca acctcgatat gaataatgat tcctgactga aagttttcca
     3121 aaggaaccaa atgattttgt gacaacataa ggcagtgact accaatcgta aactgagcaa
     3181 aaccatcatc agcataatct gcctttttat ccaaaatatg ctccaagtca gcacagactt
     3241 ggggaacatt tgaaacgata atatctaatt gatttaaatt catttactct cctccataaa
     3301 aagaccgaat tgctccgatc ttttaaagtt ctgctctatg aaaatcaaag aataaagtct
     3361 acaagtttca tatttgattt tcggcgagag gaattattta attgcgcgtg attgcaatcc
     3421 ttcttcttcc aagaagagac ggaatggtac gagttcttct gcttcgtatt tttccttgaa
     3481 ggctttgata gcttcttctg agtgaagttt tggatccaat tcaagtactt ctactggaag
     3541 tggacggtgt tgagtgatgc gagcatcgat gacaacagtt ttaccttctt tgttcaattt
     3601 aacagcttct gcaacaactg catcgatgtc ttcgatacgg tcaactgtga atccaacagc
     3661 tccttgagct tccgcaattt tagcgtagtc agcgtttgtg aagtctacac caaacaagtg
     3721 tttgtttgta tcttcgtatt tgttcttgat gaagccgtac tcagcatttg agaagacaag
     3781 gttgataact ggaaggtcgt attgaacgtt tgtgataacg tctgggtagc acatgttgaa
     3841 tgctccatca cccatgatgt tccatacttg gcgatctgga ttgtctttct tagcagcgat
     3901 accaccagga agggcaatac ccattgtcgc aaagagcgga gatgtacgcc acatattctt
     3961 aggtgtcatg tgaaggtgac gagtagatgt ttgagtagtg ttacctacgt cgattgagta
     4021 gatagcgtct tgatcagcat gtttgttgat tgcattgtaa acttgataca attgcaattc
     4081 accctcagtt ttaccttcga gtttgttcat gtaatcacgc cagttttggt tgttcttaac
     4141 gtttgcacgc caccatggag ttgattcaac tgggtttact ttgtcaagga tagctttagc
     4201 tgcttgacca gcatcaccaa ggattgaagc gtcaagggca tgacgtttac caagtttgta
     4261 agggtcgata tcgacttgga tgaatttttc agtgttcttg aatgcttcgt aaacttcagc
     4321 aaatgggaag tttgaaccaa ggaaaagaac tgtgtctgct tcaaagacca cttcgttggc
     4381 tggtttccaa ccaacacggt aagcagaacc tgtcaaacct tcatagttcc attcgaaagc
     4441 ttcaaagttt ttaccagttg tgatgattgg tgctttgatt ttacgtgaca attcagtaat
     4501 cacttcacca gctttaacac caccaaatcc agcatagata actgggcgtt cagcattgtt
     4561 caagatttca acagctttgt cgatttcaac ttcgttcaaa gcaggagcga tgaatgagcg
     4621 ttcgtatgaa cctgaaccgt agtatgagtt ttcgtcgatt tcttggaaac cgaagtttac
     4681 tggaatttca acaacagctg gacctttttt agaaattgca gcacggcagg cttcgtcaat
     4741 tacttttggc aattgctcag cgtaagctac acgtttgttg taaacagcga taccgttgta
     4801 cattgggttt tggttaagct cttggaaagc atccatgttc aattcgttaa ctggacgtga
     4861 tccaaggatc gctaggaatg gagtgttatc catagctgca tcgtaaacac cgttaatcaa
     4921 gtgagtcgca cctggaccac ctgaaccaac tgcaaccccg attgagccgc cgaatttagc
     4981 ttgcataacc gctgcaagag cacctgtctc ttcgtggcga acttgtaaga agcggatatc
     5041 tttgtcttca gccaaagcgt ccatcaatga gctgagtgtt cctgatggga taccgtagat
     5101 tgtatctacg ccccatgttt tcaatacgtt aagcattgct gcagatgcag taattttccc
     5161 ttgagtcata atgataactc tccttcaatt tttttagatt tggagaatac gattacatag
     5221 aattggaaac gttctccaaa tttttactat tccactgtat catatttatg ctgacttttc
     5281 taaaaatctg ctcaaaactc tctattctct attctaatac agttttgaaa gttctgtcat
     5341 ttctgtttta taacaaagaa atctagtcat tacttttagt ctattttact aaaatttaac
     5401 agaagggaac tggtcagaac agatacagaa ctaaaggcca tggctagacc tgccaattct
     5461 gggttgagag ccagtccaac acctgaaaag actcctgctg caatcggaat tccgacaaca
     5521 ttgtagataa aagcccagaa aagattgagt agaattcgat gaaaggtttt cttactcata
     5581 tcaaaggcac gaaccactcc taaaagatta ttggttgtca acaccaaatc tgctgactcg
     5641 atggcgatat ctgttccagc tcccatagca atccccacat ctgctacact aagggcagga
     5701 gcgtcattga taccgtcccc aacaaaggct actttccctg actgttgcag tttatggatt
     5761 tcatgggctt tttcttctgg caagacgcct gcaatgacct cttcaattcc gatttgatct
     5821 gcaatagcac gcgccacacc agcattgtct cctgtcagca tgactgttcg gagaccacgt
     5881 ttttttagct gactgatggc tagcttagca ttttccttag gaatatcttg caaagcaagc
     5941 aagcctttga tttcattgtc aacagctaag aacacaactg tcttagcttc tttttctagt
     6001 tcttctagtt tatcttgata agtattagaa atatccatac catccagcat tttagcattt
     6061 ccaagtaaaa cttgttttcc attgattcgc cctgaaacac ctttcccgtg caaggactga
     6121 aaattttcaa cagtttgaaa ctcaagtcca gcttcactcg ctcgcttaac gatagcctca
     6181 gccagtgggt gttgagaagc atcttccaag gaggctgcca atccaaacac ttctacttcg
     6241 tcgccgatga tatctgttac cacaggtttc ccttccgtca aagtcccggt cttatcaaag
     6301 acaagagttt gaactttctg gatttcctgt aagacagttc catttttgag gagaaccccc
     6361 atcttggcac tacgtcctgt ccccaccata agggctgtcg gtgttgcaag tcccaaggca
     6421 caaggacagg cgataatcaa aaccgccact ccgtagagaa gagaggacac aaagctagct
     6481 ccaagcacga ccacactatc cctgagcaag acgaaccaaa cccaaaaggt catgattcct
     6541 aaaatgacaa ctactgggac aaaaatccct gaaatcttat ccgtcaagtc ctgaatcggc
     6601 gcacgacttg tctgagcttt cttcacaaaa tccacaatct gagccaaaac agtctctgag
     6661 ccaacttttt ctgctctaaa gacaagcgtt ccactatgat tgatggttga gccaatgaca
     6721 gtatctccaa ctgtcttgtc cacaggcaga ctctcacctg tcaccatgga ttcgtcaata
     6781 ctagagacac cttctactac gacaccatca acagcaatct tttcaccggg acgcactcga
     6841 atcaggtcgc ctaccttgac ttgttccaaa ggaacttgga cataactatc atcactcaag
     6901 acttctgcgg ttttagcttg caagtccagt aatttctcca cagcttggga cgtatttttt
     6961 ctcatttttt cctcaaaaac tgctcccaaa agaacgaaaa agaggataaa tccagcactt
     7021 tcgaagtaaa cagggagacc agcaaagaga gcaactaggc tatagaaata agccactaga
     7081 gttcccagcg caaccaaggt atccatgttg gcattgtgct ttttaaaact ggcccaagca
     7141 ctctggatat atggcttacc tgcaactaac ataataggcg ttgttgctag aaaggttccc
     7201 caatgcatga cttgatgact aatgctacct gtcaacatcc caatcatgag aatcacaaga
     7261 ggcacagtaa agatactagt aatccaaaaa cgttgcagga gagatagaga ttttcgagtc
     7321 ttctcaacga ctatatagct tcccttttgc atcttcatgc cacaagaaaa ttcatgtcgc
     7381 cctaattctt gaggtgtaaa acgaatgact ttctcctcat ctacgccgat tggttccaag
     7441 ataccttctt cttcaaacag aatttcctta taacagtttg aaggagtagc acgatgaaag
     7501 gtaatctcag ctggaattcc cttttgaagc tggatatggg ctggatgata gcctttttca
     7561 gctcggatac ggattttttg aatgccattt tctaagcttg ctttcacaat ttctgtcata
     7621 gtctccacct actctacaat catcttgccg tgcatcatgt tcataccaca agcaaagcca
     7681 aactctccaa cctgctcagg cgtgatttcc actacatact cttcccccat tggcaggttc
     7741 gcatgtacac caaaatctgg aaaaacaatt tgatccagac atggtgaagg atccttgcgg
     7801 tcaaagacaa tgcgtgctgg cactgatttc ttgaggacaa tcaactcagg agtatagcct
     7861 cccatgactt ccactcgaat ctcttggtat ccgttttttt gctgggcttt ttgtccagat
     7921 ttttcaggct ttttgaaaaa ccaaaacaag ataaacgcga taagggcaat acaaataatg
     7981 gttacaatac tatttaacat gacgtctcct ttacatacaa ttacatctta cttctgttac
     8041 agcacttgat ttcttctctg aaatcacagc ttccaagtct tccaagtcag tctgagtaaa
     8101 ttcacattct acaatcaagt cagccaacaa attcctaatc ctacgggaac aaaccttgtc
     8161 tttgatatct tggacaagta aatcccgact ttggtctaga gttaaaaggg ctgaataaac
     8221 aaaggacttg ccttcttttt tccgagtcag acactctttc tcaaccaaac gagccaaaag
     8281 agtttgaatg gtcgacttgg accagtcgaa ccgctctgcc aaaaccctaa tcaaatctgt
     8341 actggtctgc tccccctgca tccaaataat cttcatgacc tgccattctg catctgaaat
     8401 ctgcattacc atacctccaa aatctacatt tgtcaattac actcatcagt atactcttaa
     8461 aatctacatt tgtcaattat agaaataata ttttcttcga aaaatagaat tttaatcatt
     8521 tgaaaaacga tttgcagtca aatattacta tataaacaat aaaaatatgc tatactaaag
     8581 aaaaaagaaa acaaccacta ggggtgcgta aagctgagat taacgactgt tagatccctc
     8641 tgactcaatc taggtaatgc tagctgatgg aagtggaaat gataatgggg actagcagtc
     8701 ttctattgcc tttctaaaac agactagctt gttcttaaga atacaaactt cagttggttg
     8761 ggaggtttta gatgacttat ttacccgttg ctttgaccat tgcagggact gaccctagtg
     8821 gtggtgctgg cattatggca gatttaaagt cattccaagc gagagatgtc tatggaatgg
     8881 ctgttgtaac cagtcttgtc gctcaaaata ccagaggtgt tcagctaatc gagcacgttt
     8941 ctcctcaaat gttgaaagcc caattggaga gtgtcttttc tgatattcca cctcaggctg
     9001 taaaaactgg aatgttggct actactgaaa tcatggaaat catccaaccc tatcttaaaa
     9061 aactggattg tccctatgtc cttgatcctg ttatggttgc tacaagtgga gatgccttga
     9121 ttgactcaag tgctagagac tatctcaaaa caaacttact acctctagca actattatta
     9181 cgccaaatct tcctgaagca gaagagattg ttggtttttc aatccatgac cccgaaggca
     9241 tgcagcgtgc tggtcgcctg attttaaaag aatttggtcc tcagtctgtg gttatcaaag
     9301 gcggacatct caaaggtggt gctaaagatt tcctctttac caagaatgaa caatttgtct
     9361 gggaaagccc acgaattcaa acctgtcaca cccatggtac tggatgtacc tttgctgcag
     9421 tgattactgc tgaactagcc aagggcaaga gtctttacca ggcagttgat aaggccaagg
     9481 cctttatcac aaaagctatt caagatgccc ctcaactcgg tcatggttct ggtccagtca
     9541 accatacaac ttttaaagat taagaaaaaa actctctagt tcccacttta agggaattag
     9601 agagttttta tactcttcga aaatctcttc aaattacgtc agcttccatc tgcaacctca
     9661 aaacactgtt ttgagctgac ttcgtcagtc ttatctaaaa cctcaaggca gtactttgag
     9721 caacctgcga ctagctttct agtttactct ttgattttca ttgagtatta attaggaaag
     9781 aatgtcatgc aactttgtta aaaaggcttg cgtttttgcc tcaatatctt ctgcttgcat
     9841 caaatcacgt acaacagcta caccagctat gccagtgccc ataagctgat caatattctc
     9901 cgaagtcaag cctccaatag caactactgg aatggcaacc gtttggcaaa ttgttttcaa
     9961 ggtcgatatc agagtaatgg gcgcattttc cttggtggtg gttgggaaaa tggctcctgt
    10021 acccaagtaa tctgcacctg atttctccgc ttccagagct cttttaaccg ttttagcggt
    10081 gacaccgagg attttttcag gacccaagac tttgcgagct accgaaactg gtaattcatc
    10141 atctccgata tgcagacctg ctgcatcaac cgcaagacaa acatccaacc gatcatcgat
    10201 tatcaagggg acctgatagg catctgttat ttccttgact tgttttgcca gttgataata
    10261 ttgattggtt gtgagatttt tttctcgcaa ttggactatg gtaacccctg aacggcaggc
    10321 cgtctcaact tttgcaagaa agctttccac ggaatcttga tagcgattgg ttaccagata
    10381 tagtctaagt gcttctctat tcataaacct ctcctttgat ggtatctagc caattttcat
    10441 ctcttcttag gagcgaaagc tgattgagta cttggtaacg aaattcttcc aatcccattc
    10501 cttgaacaac tattttctca gcagcgatat tgagataaga gactgctaag caagaagctt
    10561 caaaaccagt ctttccttgg ctgagaaaaa cggctgttaa ggctccaacc aagtctcctg
    10621 tccctgttat ccagtctaat tcagtacagc cattctccag tacagcaact tgattctccg
    10681 aaacaataag gtccttgggg ccagtaacta agaaagacat accaggatag gtctgacacc
    10741 agtctttcaa gacttgaagc aaatcctccg tttcttgatc tttagcactc gcatcgaccc
    10801 caacgccgtg gtgctttaat ccaacaagac ttcgaatttc tgacatgttt cctttaagga
    10861 ccgtaggtct atagtctaaa aggtctttaa ctaagctctt acgaatggat gaagtcgtta
    10921 cgccaaccgc atctactacc atcgggagag aagattggtt tgcatacgaa gctgccatgc
    10981 ggattgcttt ttccttctca gctgacaaat gccccaaatt gatgaagaga gcctgacttt
    11041 gcttagtaaa atcaagaact tcacggggat catctgccat gacaggtttg catcccagag
    11101 ccaaaatccc atttgccagc atctcacaag aaatctcatt ggtcatacag tgaatgaggg
    11161 aactagagcc tataggaaaa ggatttgtca atgcctgcat cagtctatcc tttcagcaaa
    11221 gaaatatcct tgcacttttt taaagaattc ctgcttgatt aaaaatctaa atgcaataaa
    11281 ggaaatcgct gtaccaatca aggttgctcc gaaaaatcga ggagtgtaga taaaccaact
    11341 aagcttagca gccgatcctg taaagagcac cataacagga taggaaacaa tagaaccaat
    11401 aatacctgtt cccacaattt ctcccaaggc agaaaagtaa aattttcgac cgtacttata
    11461 aaagagacct gctagaaggg ctccaaaagt cgctcctgtg agagctaaag gcggaatccc
    11521 ttgagtcgtc atacggataa aggctgtcac tgtagccata gccaaggcat aaacaggtcc
    11581 catcatgatt cccgctagaa tattgactac actggacatc ggtgccattc cctcaatccg
    11641 aaagataggt gtaaggacta catcaagggc aatcatcata gataaaatgg tcaatttgtg
    11701 aacttgtagt tggtgctttc tcatgtttct attcttctcc tttttctaaa gactgtaaat
    11761 cgctcttcca tgtctggtgt tggtaagcca tctcccaaaa cttggcttcc atatgaacac
    11821 tgatgtggaa ggcatctagc attttttgct tatctgtctc atcactttct cgataaagct
    11881 gattgaccag tgctccctcc tctctgatct gttgctctaa ctcatccgta atataagttt
    11941 caatccattg ttgatagaga ggatttggtg atggtttaag attaagtgat ttgcctatat
    12001 catggtataa ccaaggacaa ggaagcaagc ttgcaaaagc tatggctaag ttcggttctg
    12061 caaattgcct ataaatatga gaaatgtaat gataacaggt tggagcgatt ggatgttgct
    12121 ccatttcctg gtcgttgatt tccaattcct tgaaaaattg ttggcgaata aataactcac
    12181 cctccactaa accctgagca ttttgtttca agagtctttt catctcttgg tttgaagtct
    12241 tatcagccaa aagatgatag atttctgaga aagccttcag atagtaggca tcctgaatca
    12301 ggtaatagcg gaaaatggca ggttctaaat tcccctcttg taattgtaaa ataaagggat
    12361 gatgaaagga agcctgccaa gctttcttgg ataattccat cgcaatatct gtaaattcca
    12421 taataactcc tttataaaaa tagactggtt tgaagcaata aaaagaaaag caggtagatt
    12481 aattttgttt ttttaggaat ataaaaagtc cgatagctat tcttcaactg tgcatgttcg
    12541 tcatatccgt aagcagatag agctctcagg taaagatggc gccacctaaa gactgtcatc
    12601 agaaccttac tgtaaatcaa gggcgaccaa aaatgtagtt tttgaccacg taataggcaa
    12661 gcttctttga gggacttgat ttcttgctga atgagaggaa aagaattgaa taccacaatc
    12721 aaggcatagg accaagagcg tgatagcccc ttttgagcca agtacaagag aagctctttt
    12781 agtgaaatag aggaaacaaa gacaaggccg atacaaactg tcacaaaggc cctcgttcca
    12841 agcatgactg cctgtgaagc atctccgtgt aactgaactg cccagtagtt ggcaaaagat
    12901 gggaaaatgg caagtatgat catccaagct aacattttaa atcgacggta atagagcata
    12961 aagagaatac aaaatgcgac taccgaaaga ttcagagcaa tcgaaggaat gaaagatgtt
    13021 tccaaggata aaatcagcaa gaagagactg ataatcggtg tctgggttgc tactttgacc
    13081 atactatctc acctcccctt gggtattgct actctgagat gtaagtggtt tggtaatggt
    13141 cacttctttc acatgccgaa gaccctgact agtcatctca atccaataat caaccacaga
    13201 aatcaaaggg tctaaacgat gactaatgag cagaaaactt cttccttgat tcctctcctc
    13261 cacaatccac ttgcaaaaat aatggcaggc tctatcatcc aaacctgcaa aaggttcatc
    13321 tagcaagatc acggaagcct tactggtcaa gatggtcagg agctgaagaa ttttttgctg
    13381 accaccactt aattgatagg gactcttatc gactgcctgc tccaaatcaa aatatcgtaa
    13441 agcttgaaaa atccgctgat ttctttcaga atcaggtcca tctaattgaa gctcctctcg
    13501 cagactgact cggataaact gcttctcagc ttcctgaaca acaccagtca gatcacgata
    13561 caaactcttt ttctttttca ggaccgaacc cttccaagta atgctcccct tatacttttg
    13621 aaattgaaga atagaccgaa agagggttga tttcccgaca ccattgtcac ccaggataca
    13681 ggaaatccct tgatagaatg tgaaatcagc aattgaaaag agggggcgat taccaagctc
    13741 accagtcaca cggttcatat ggaatagttc cgggctagaa gcaacttcct ttgaagcaac
    13801 ctgtgtcatc tcataggaag ggatttgaaa cacttccctt agttttccgt ctcttagctc
    13861 caccatatgg tcgatatagg ctttatagtc agataaatca tggtcgcaca aaataactgt
    13921 cttcccatca tagaccaact cttttagaat ctccaatatc tcgattctgc tcttgcggtc
    13981 aatggaagcg aagggctcat ccaagagata gaccctagga ttcatggcaa agaggacagc
    14041 cagcgctgct ttttgctttt ccccacctga taagtgatgg atgagacggt gcaagatgtc
    14101 cttgcaacga cattgctgga caacctctgc tattttagaa tcaatttcct gaaggtgata
    14161 gccgatattt tccatggtaa aaaccaactc ctcaaacaag ctctccatgg taaattgatg
    14221 attaggattt tgcaagagaa taccaaccgt ctggacacgt tcgacgatag aaagctgact
    14281 gacctcgctc ccatctatca ggacttgacc gctataggga agagaactaa cttgggcaat
    14341 catttgaaag aggctggatt ttccagaccc actactccca actaacaagg taaaggcttg
    14401 cgcatgaaaa gtaaaatcaa acggctcaga gaagattggg gactgaatcg ctcgtagttc
    14461 cagacccatc tatgcttttc ctccagttgc aaactgatga tagagtttga caatggcacg
    14521 aaccaagatg gtacagaaga aataaacaga aataaaacgt accacaagca aggaaaggac
    14581 aaacggaagg gaaaaggcgt agtaacctaa cttaatgtat tcatagacaa agctaacaag
    14641 cgtaatccca atactattag cagttagaga gagccaactt tcatagcgat tcttagttac
    14701 gataaaacca aattcacttc ccaaaccttg aacaaagcca gacaaaagag ctcctagacc
    14761 aaattggcta ccataaagga cttcagcaag cgcagctagc acttctccaa tcgttgcact
    14821 tccgactctc ggaacaaaga tggcagcaat gggcgcagcc atacaccaga gaccgaagag
    14881 gatttcattg gcaaaggcct gcaaaccaag aggtgttaag agtagactga gaatattata
    14941 cacatatcct gaaccaacga aaaccccacc aaaaaagata gacaagaaag caagcaagat
    15001 aacatctttt aactgccatt ttttcaacat aaaaaactcc tttttttaaa gaaaagtggg
    15061 gcactcaaga agaccgacct aaatactttg tatagcagac tgaatttaga acagtacaca
    15121 agaacactaa aatatttcta gaaattaatt tgaattttct aattgatttg ttcgcatctt
    15181 atttcaatct actatatcat cttcatccag tttcgtaaaa gaaaaaactc taattacaga
    15241 tacaaattag agttcagctt acaagattag acagttcttt tcgacatacg aaaaaaacat
    15301 ttcacatttc ccttcgccag tcttaactgt atcaggttca atgggtatca tctcagccta
    15361 aagcacccca aatgtcttta ttatttaatt atgtgattat tataacacac attttatact
    15421 agttcaagaa attgaactgg aaatacagcc ttgcactcac aaagacagca gatctttctt
    15481 ttgcaaaaaa caaatgacct gtttgatgaa ttagccattc aagctgaatc tggacatagc
    15541 tttttaaaaa aggaaaatcc tacttactta gaatccaagg atagatatct attgttcact
    15601 catttcccga acagtttttt ctatattttt tgcatacgat attgccgaaa tgattgaaac
    15661 gccatccata ttggtcttta taatgtcttt aatatgtttc gtctgtatcc caccaattgc
    15721 aactaaaggc atttgtggca atagttttct catcaattca agaccttcat aacctatagt
    15781 accaccagca tcatcctttg actgggtacc aaatacaggc ccaacaccta cataatctac
    15841 atattcaact tttgattgtt gaaattcttc ttcgtttctt atagaaagac caattatttt
    15901 atctggcatc aattttctaa tttcatcaac accaatatca tcttgaccta catgtacgcc
    15961 atcggcgtca atttccattg ctaaatctat atcgtcatta acgataaatg gaacattgta
    16021 ttttttacaa agttctttaa tttggatagc tagctcaagt ttttctaagc cttctaaagc
    16081 accctcacct ttttctcgaa attgaaataa ggttatacca ccttttaagg cttcctcaac
    16141 gactgtatat agattttttc cttggcaagt agtcgttcca caaataaaat atagttttag
    16201 taattcttta tgaaacatct tacttcactc ttttgaattc ctttacatct tcatctgtaa
    16261 tctcgtataa ggcatttata aattcaactt taaatgtccc aggaagatgt ccatttggac
    16321 gtttttctgc catttctcca gcgatattgt aaaccaacat tgctgtttct aatgatttca
    16381 attcttgacc tttttctagt ccgataaagc ttgctactac agctcctaat aagcatcctg
    16441 tcccaatgac tttcggcatc atagcactac cattatgaat cattaccact tctccattaa
    16501 cagcaatggc atccacttca cctgttacta ctattggaat attgaacttc tcatttgctg
    16561 ctagagcaat ttcgtcaata ttatctacgc ccgcactatc tactccttta gatgccacat
    16621 ctattcctac taaagaggca atctcgccag catttcctct aatcgctgct agtttataat
    16681 tgttgattag atcatctgct actttttttc tatattctcc tgctccacag gctacaggat
    16741 ctaaaactgc tgggacatta tatttctctg caattttcag agcagcttgg tataatttcc
    16801 aattttcatc tgtcaatgtt cctatgttta ttaataaacc accagcatac tttaacaaat
    16861 cctctaaatc tgctggaaac tcactcatgg ctggtgaggc gcccagtgct actaatccat
    16921 ttgctgtgaa attttttact acatcattgg ttatacaaat gaccaatggt gctttttctt
    16981 ttaataattt taaacttgtc atattgaaat ccttcctttt cactttatac gatctactga
    17041 tttcgattta tttctatctt tcgttaagaa tttttttcat ttacattgaa tgatttatac
    17101 tcaatgaaaa tcaaagagca aactaggagg ctaaccgcag gttgctcaaa acactgtttt
    17161 gaggttgtgg atagaactga cgtggtttga agagattttc gaagagtctt acctcatcaa
    17221 atttgtaaat atcatgagcc ttctctagac atcgtaacca atatcaaaaa aagctaattc
    17281 taaagcgact gcttgattcc agcgttgctg aagttctgtc aaatcttctc gatttttatc
    17341 gacacgattg agttcgtcaa ccagaaattg aacccactct gcaaagaaag gacctctgtg
    17401 gagattgatc cattccgaat gaatatagac ttcaggtaaa gccaaatctt tagaacccca
    17461 gtctaaatag agaccttctg caatgaccag catgaccaaa agatgggcat agtctgatga
    17521 agccaccgcc gaatacatta gatcctgaaa ggcttttgtt acagggtgca aagtcacttc
    17581 tagatagtca ttctctgcta cttttaactc tttaaaagcc ttttggaaat aaccatcttc
    17641 atctgcttca agaaagccta gttgcttggc aaaacgaagc ttggattcaa gtttatctgc
    17701 gtgagctacg caggcaccca gcatggataa gaaggcatca aagaagtgat aatcttgaat
    17761 cagatagtcc tttaagacct tattctcaat tgtccccgca aaaagttcct taacaaaacg
    17821 atgattgatt gcagcctgcc aatccttctg actgcttttt aataattctc caacagtcaa
    17881 acctggctga aatgcatagt cttgtgtttc catatttact tctcctctct ttacttgtta
    17941 gtaattaata aaacaccaag aaatatcaag caaaatcgta attccacttg atccttttaa
    18001 agcacatcga gagcatttgc agagagctaa ctaaacaagc ctatccagtt tatataaaca
    18061 aaaaactcca attacaatca agaattagag ttgacttaca agattagatc gttcatttcg
    18121 ccatacgaaa aaacgtttca catttccctt cgccagtctt aactgtatca ggttcaatgg
    18181 gtattatctc agcctaaagc accccaaatg tctctattat ttaattactg aaccagtata
    18241 gcaaaaaatg aaagccctag caagatattt gaccgaaaaa tatctttata tataatatat
    18301 tgaaactaga atagtacacc tctacttcta aaacattgtt agaaatcgat ttgactgtcc
    18361 tgattgattt gtcctattct tatttcattt tactatagtt ttcgatagca atttattctt
    18421 ccaatacacg aagaaaaacc tccacattca gtggaggcaa tctgttttat caatacaatt
    18481 ttaagtcacg agggtcaact gggaaggttg ggttgtatgg attgtgacgg agcttaaagt
    18541 gtttgacatc ttcaatggtc tgagctccag acaattgcat aactgtcttc aattccgcat
    18601 tcaagtgttc aaagacttga cgcacaccga cactaccacc gagagccaag ccataaataa
    18661 cagggcgacc aatagctacc aagtctgctc ctgaagccaa ggctttaaag acgtgttgac
    18721 cacgacgaac accagagtca aagacaatcg gcacacgtct atcaactgct tctgccactt
    18781 cttgaagcga gtcaaaggca gctggtccac cgtcgatttg acgaccaccg tggttggtta
    18841 cccagatacc agaagctcct gcagcaagtg aacgttcaac gtcctcacgg cattgtggtc
    18901 ccttgacata cacaggaaga ccagagtatt cagcgataaa ttctacatcg cgtggagaca
    18961 agcgttgttt agctgatttg taaacaaagt ccattgattt accagcacct tctggcaggt
    19021 attcttcaac aatcggcatg ccaactggga agacaaaacc attacgctta tccacttcac
    19081 gattcccccc tacagtagca tctgccgtca agacaatcgc tttataacct tcagccttca
    19141 cacggtccat gatgtggcgg ttgataccgt catccttact aaagtaaaat tggaaccaat
    19201 ggggtgtccc ttgaagggct tcagaaatct ctggaaggtc aacagtagag taagagctgg
    19261 ttgtataaag agaaccaaac tcatgcacac cacgcgcagt cgccacttcc ccctgttcat
    19321 ttgccaattt atgagccgca acaggcgcca taatgattgg tgaagatagt ttttcacctg
    19381 caaattcaat ctctgtactt ggattttcaa catcgcaaag tgtatgagga acgatgagtt
    19441 tgtggttaaa ggcgcggata ttctctctta aagtgaaagt atcttccgcc ccactagcga
    19501 tatagccaaa tgctgcttta ggaataactt gttgcgccat tggctccaaa tcataggtat
    19561 tgatgaagtc tacatgacct tctgcattgc ttgttttgta tgacataaaa tgccctcctt
    19621 gattaagtaa gcgtttgctt tttactacac aactataata actctttttt caatcatttt
    19681 caagagtttt gcttaaaaac catcttattt tttcatacaa tatactagcc ctactttcct
    19741 taaactctaa actttagtct atattaagtg aatcgttgag attttttgct gccaggcatt
    19801 tgaactactt ttttatttgc ggattctatc tactaatact ataaaaaaac aagtgaataa
    19861 gacaccagaa catcctctaa taggacctat ggacttatat cagtcccata tatactgttt
    19921 aagcaagata ctatcctagt tgacgctaac ttcctatccc acttattaat tttttattta
    19981 tagtgaatct gaacaaatca attggggaat ccaagtcaat ttctagtact ttttgaacag
    20041 tttcagcgta ctatcgtaaa atgaaataaa acatgcacaa attgcttagg gaatttaaac
    20101 caatttctaa caatgtctta gaaatcaaag tgtactattt caacttcaat atactacact
    20161 tcattttctc cttaaattca gtctcttttt gaatcaaaaa atcccctggt aagagccaga
    20221 ggataagaat ttatttcatt gttgggaaga gcaatacatc acggatagtt gttgtatcag
    20281 tgaggagcat gcagagacgg tcgataccga ttcccaaacc acctgttggt ggcataccgt
    20341 attcaagagc ttcaatgtag tcatagtcga tgcctgtcgc ttcatcatca ccaagttctt
    20401 tggctttagc ttgggcttca aaacggctaa gttggtcgat tgggtcgttc aactcagtaa
    20461 aggcattacc gtactcctta gtcatgataa agagctcgaa acggtcagta aagcgttggt
    20521 cttcaggatt tttcttagcg agtggagata cagctactgg atgtccatag acaaaggttg
    20581 gttggattaa agtttcttca acaaactctt caaagaaggc attgatgatg tgaccaacct
    20641 cagtgtagtg tttctcaact ggaactttct tctcagcagc gatagtttta gcttcttcca
    20701 aagtcatgtc ttgccagaaa tcgacaccag taatttctct gatagcatcc accatatgaa
    20761 cacgcttaaa tggttcgtta atcttgattt cagtaccttg gtagttgact gggccatcac
    20821 ctttgactga tttagcagcg tgttggataa tgccttcagt caagtccatg atgtcttgga
    20881 agtctgcata agcttggtaa acttcgatag aagtgaactc agggttatga gtagcgtcca
    20941 ttccttcgtt acggaagata cggccaattt catagacacg ttccatacca cccacgataa
    21001 ggcgtttcaa gtgaagctca gtcgcgatac gaagcaccat gtcaatgttt tgggcattgt
    21061 ggtgggtgat aaatggacgg gcagcagcac caccggcttc attatgaaga acaggtgttt
    21121 ccacttcaag gaatcctttt tggtcaaggt aacgacggat ttcagagatg atttttgaac
    21181 gagtgacaaa gcgttcaaag ctttcacgat tagaaatcaa gtcaaggtaa cgtttacggt
    21241 aaattgtttc aacgtctgtc aaaccatgga atttctcagg aagaggacga agagccttag
    21301 acaagtgtgt gatgtgggtt gccttgatag agagttctcc catatccgta cgcatcactt
    21361 caccttcgac accaaggaag tcaccaaggt ctgctttttt gaagatttcg tagttttctt
    21421 caccgacagc atccttacga acgtagatct gaatctggcc ttcgcggtct tgaaggtggg
    21481 caaaaccaac ttttccttta ccacgtttgg ttatcaagcg tcctgcgata gtagctgttt
    21541 cgtttttatc gtgtaattgt tctttatcga ggttggcata tttatctttt aattcttgtg
    21601 aatttgcagt acgttcaaaa cgttttccga aaggatcgat tccttgttcg cggagcgcag
    21661 ccattttttc acggcgaacg atctgctggt catttagttc ttccatatgt tctgtagaca
    21721 tgggatcctc ctagttttac tcaaaattga atacgatgag tcattttttg cgatactccc
    21781 attttatcat aaataagcaa gaaattcacg gataatcttt aaaaactaaa aagaacctga
    21841 cgctaaaatc actggaaaga agtgaaagta tctcccgccc cactagcgat atagccaaat
    21901 gctgctttag gaataacttg ttgcgccatt ggctccaaat cataggtatt gatgaaatct
    21961 acatgacctt ctgcattgct tgttttgtat gacataaaat gtcctcctta ataagtaagc
    22021 gtttactttg tgtattacaa aaatatctta actctttttc aaaactttta aaatattttg
    22081 tttggaaatt tcagaaattt tatgtctatg ataaaaatcc ttataacggc aataaaaaat
    22141 agatattatc caaagaagat tttaagtgct acaataactg tattatttct agatgggagg
    22201 ttctattttt ggattgatcc attgttgaac aatatctacc actatatcaa aaggcattct
    22261 ttctgacctt gcatattgca gtttggggaa ttttgggatc ctttctgctc ggtttaatcg
    22321 ttagtatcat ccgacattat cgaatccttg ttttggcgca agtagcgaca gcctacattg
    22381 aattgtcacg taatacgccc cttttgattc aactcttctt tctctacttc ggtcttcccc
    22441 gaatcgggat tgtcctatct tcagaagtct gtgcaacgct tgggcttgtc tttttaggag
    22501 gctcctatat ggcagaatct ttccgaagtg ggctggaagc catcagtcaa acccagcagg
    22561 agattggcct cgctattggt ctgacacctc tacaggtctt ttactatgtg gttcttccgc
    22621 aagcaacagc ggtggcactc ccctccttta gtgccaatgt cattttcctt atcaaggaaa
    22681 cctctgtttt ctcagcagtg gctttggccg acctcatgta cgtcgccaag gatttgattg
    22741 gtctctacta tgagacagac attgcgctag ctatgttggt agttgcttat ctgatcatgc
    22801 tgctacccat ctcactggtc tttagctgga tagaaaggag gctccgccat gcaggattcg
    22861 ggaatccaag tactctttca aggaaataat ctcctgagaa tcttacaggg attgggcgtt
    22921 acgattgaga tatccatcct gtctgtcctc ttatccatga tgttcggaac agtcatggga
    22981 atcatcatga cctcccattc tagaatcata cgatttttaa cacgattgta tctggacttt
    23041 acccgtatca tgccccagct ggtgctactc ttcatcgttt actttggctt agctcgaaac
    23101 tttaatatca atatctcagg tgagacttca gctattatcg tttttaccct ctggggagca
    23161 gctgaaatgg gagacttggt acgtggagct atcacttctc tccctaaaca tcagtttgaa
    23221 agtggacagg cactcggctt gactaatgtt caactttact accacatcat catcccacaa
    23281 gtcttaagaa gactgctacc gcaggctatc aatcttgtca ctcggatgat taaaaccact
    23341 tcattagttg ttttgattgg ggttgtggaa gtgaccaaag ttggacaaca aatcatcgat
    23401 agcaatcgcc tgaccatccc aactgcttca ttttggattt atggaaccat tctagtctta
    23461 tatttcgcag tttgctaccc tatttccaaa ctatccactc acttagaaaa acattggaga
    23521 aactaaatgt ctgaaactat cttagaaatc aaggaactaa aaaaatcctt cggagacaat
    23581 cccatcctcc aaggactttc tctagaaatc aaaaaaggga agttgttgtc atcctagggc
    23641 catctggttg tgggaaaagt accctccttc gttgcctcaa cggcttagaa agtattcaag
    23701 gtggagatat tcttctggat ggtcagtcta tcgttgaaaa taaaaaagat tttcacctag
    23761 ttcgccaaaa gattggcatg gtctttcaaa gttatgaact ctttccccat ctggatgtct
    23821 tacaaaacct catcctaggc cctatcaaag ctcaaggaag ggacaagaaa gaagtaacgg
    23881 aagaagcttt gcaattacta gagcgtgtcg gtttgctgga taaacaacat agctttgccc
    23941 gtcaattatc tggtggacag aagcaacgtg ttgcaattgt ccgtgccctc ctaatgcatc
    24001 cagaaatcat cctttttgac gaggtgactg cttcgctgga tccagaaatg gtgcgtgagg
    24061 tgctggaact tatcaatgat ttggcccaag aaggccgtac catgatttta gtaacccacg
    24121 aaatgcagtt tgcccaagcc attgctgacc ggattatctt cctcgaccaa gggaaaatcg
    24181 ctgaagaagg aacagctcaa gccttcttta ccaatccgca aaccaaacga gcccaggaat
    24241 ttttaaacgt ctttgacttt agccaattcg gctcatatct ataaaggaga ttcttatgaa
    24301 actattcaaa ccactcttaa ctgttttagc acttgccttt gcccttatct ttatcactgc
    24361 ttgtagctca ggtggaaacg ctggttcatc ctctggaaaa acaactgcca aagctcgcac
    24421 tatcgatgaa atcaaaaaaa aagcggtgaa ctgcgaatcg ccgtgtttgg agataaaaaa
    24481 ccgtttggct acgttgacaa tgatggttct taccaaggct acgatattga actagggaac
    24541 caactagctc aagaccttgg tgtcaaggtt aaatacattt cagtcgatgc tgccaaccgt
    24601 gcggaatact tgatttcaaa caaggtagat attactcttg ctaactttac agtaactgac
    24661 gaacgtaaga aacaagttga ttttgccctt ccatatatga aagtttctct gggtgtcgta
    24721 tcacctaaga ctggtctcat tacagacgtc aaacaacttg aaggtaaaac cttaattgtc
    24781 acaaaaggaa cgactgctga gacttatttt gaaaagaatc atccagaaat caaactccaa
    24841 aaatacgacc aatacagtga ctcttaccaa gctcttcttg acggacgtgg agatgccttt
    24901 tcaactgaca atacggaagt tctagcttgg gcgcttgaaa ataaaggatt tgaagtagga
    24961 attacttccc tcggtgatcc cgataccatt gcggcagcag ttcaaaaagg caaccaagaa
    25021 ttgctagact tcatcaataa agatattgaa aaattagcaa ggaaaacttc ttccacaagg
    25081 cctatgaaaa aacacttcac ccaacctacg gtgacgctgc taaagcagat gacctggttg
    25141 ttgaaggtgg tcattaactc ttaaaaggga ctggatttta agctccaatc cctttttaag
    25201 attttaccta taacatcctg agtctatcta agatgttcaa tctgaacaca gtgtacatac
    25261 tttatcttct attgcatata ctttatcaca taagatacga atatcctctt cactatgact
    25321 agcaatcaaa attgttgtcc ctttttcact agagagcttt ctaaacaatg ttctcatatt
    25381 ttctacactt gatttatcca aggcattcat aggttcatct agtaaaagaa tagagggatt
    25441 ctccatgatt gcttgagcaa tccctagctt tttcctcata cctagcgaat aagttttaac
    25501 tttctggtct ttttgctcat atagaccaac tattttcagt gtatcattga tttcctgatt
    25561 accaactact cctcgtatgc ttgccaaata ttgtaaattc ttaaagccac tataataatt
    25621 tataaaacca ggttcttcaa tcaaagctcc caaattagct ggaatttttc tctcaggaac
    25681 aatattttcc ccattgatta acacttctcc ataagacgga ctatataaac cagctattaa
    25741 tttaaacaat acacttttcc ctgagccatt cgcaccagta attcctataa tttccccctg
    25801 tttacaacta aagttaaggt tttgaaaaac acatgtcttt tttaatttca actcaatatt
    25861 ttttaatata attatttcat tcattctata aacctcctct tttgacgagt gaaatagaaa
    25921 atgctttgaa aaagaaagac taaaaatagc aactgaagaa acaaatctcc ccctacatcc
    25981 ccattccctc gattcaaaat ataaaataga taattagttc gatttcctac aaatagacca
    26041 ccaaacacaa tcatgagtaa aaagaaacta acgcaagcaa agttcatttg tacattcgct
    26101 cctagtgcgt ataacataaa gcaaaacaag actaacacac aagcattcaa agaatcaact
    26161 ataaacaaaa ctcctatatc ccagtttggt aactgtaaca taaattgaat cactagaact
    26221 tttaccatga ttaaaatgag tccataaaga aaacaggtcc aaatctgata cctaatccag
    26281 agcttaatag aagaaaatcg aatcacttcc ataaagaaat cggatgctct tctacgatat
    26341 acaactgcta gaaaaaatgt atgaataata aaccaatgaa gcaaccatat agataagact
    26401 aaaaaagagt tttgactcag attaggccct tttaaaattt ctataacaag tctatcattt
    26461 ccttctaacc aataccataa tcctaaacaa atcaatatcc aaaaaagaac ataaccccaa
    26521 aagtacttgg tatagattat cttattcaac ctccacatgt gactacttaa ctttcccatt
    26581 tccaactttt acactctcct ccagtttcta tgaacagaaa atacagtcaa tggaattatg
    26641 atagatagta atgtaagcca ataaatcaat gcatacggat tggaatttac catatactga
    26701 aaaattggca ataatccaat tatcccattg ttcaactgat aaatccagaa taaggattct
    26761 acaaataaaa aagtaaacag aaatactaag ctcatgtttg attttttaaa ccaagaaaaa
    26821 ctgctaatag taagtaagga taaaaagaaa tagtatgcta tataagagaa aaaaaatcct
    26881 ataaagaaac tagcattgtt tgcaatactt ataccataaa attctcttaa aaaatcaacc
    26941 tcctttatct ccaaagagaa gctaaaacca ttactaaatg caattagaaa aatcaataaa
    27001 aataaagtcg ccgtccaaat ccccgtacta agagctgcta atttgaaact aaaactggta
    27061 aagtgcttaa ttgatttcag acgaatgcga cactccaacc tattaaaata gttattcatc
    27121 aaataaaaaa agaacaatat atatgtgaac ggaaagcaat atactccagt cgtcatatct
    27181 tgaagtaaaa ctaaaatcca ttctaataca tttggatgga ttgaatattg gcgacagcgc
    27241 aataaatata ctgtactaga taaaacacag gatagcagta atataaaata aaccaatact
    27301 gataaaaaat cttttttgta aaattgaaca aattgtttca ttatacatag tcctctgaat
    27361 gtagaaaaaa tgtaccataa acaaccaaac aactaacaaa taaaataaaa gcaagatgcc
    27421 cactaactaa ggaaagactg atatctttct gatatcccaa agctaatgtt gtcacaggtt
    27481 ctaagtaaga tagccctaaa atagcccaaa aaataccacc aaccatcata taggcaactg
    27541 ggatgaaaat agctcctatt tttttcttca ctagcaaagc actagctagt ccaaaaatag
    27601 agaacacagc gccccaaact ccataccaga gagtcgtcac aagactatag agcaactgat
    27661 tagaatcaaa taattctttt aaggcaccac tataatctcc aatataaatt tcctgataag
    27721 gagtcactaa aagattaatt cctaataata ataaataggg gagaaaaaag actagaaaag
    27781 aagaaataat tgcagtacta cctacaatag ccagatactt ctttttagaa attcggcaca
    27841 attgtgctgt tagaaaatga ctctcagcat cctctattat ctgactagaa tagggcagtg
    27901 tacagataag tgcagctact aggctaatcg gtgaaaatcc tcgaatagaa gaagcggcaa
    27961 aaaaaatcga taaaccttca attttataaa taccattgaa agcaaggaaa tttcctaaac
    28021 tcatgcaaag aagggtcaaa aataaagaca tataaaatcg aggtgattga acgactcgta
    28081 caagattaca aatgaaaaat tccatcctta ctcctcctta taataaaaat agggtgtagc
    28141 attctttttt catgctacac ccacaatcaa ccatctttaa ggcttactct gacaagtaag
    28201 ttaataagaa tctggactcc aagaacctga agtatgaatt cttacatgat ttccaaattg
    28261 tggcgccata gctaatctag taccagaacc aatataattg tcaccacctc cattatagta
    28321 catgacaatc ctagagccag accccaatga atataccggg gtaatatctg acccactata
    28381 ggcgctacga atagaggtat ttaacctttt accgccacca gtgctgtcac tattattaat
    28441 tccagcagag gcgttttctt tctcaagctc actagttgta tccgaacccc aacgcggcaa
    28501 cgttaaatca taatttccaa tctgtctagc taaaactgta gggagaaaca aactagatgc
    28561 aattgtaact acgcaaaaaa ggaatatatt tttcttcttt tcatgacatc gtcctcttaa
    28621 gaagtgataa acaagatagt tgattgatca atttgataca tattacattt tcattatatc
    28681 aataaaagaa agcgttgtca attattttat ttaaaatatt gtcatatttt aaataaaatg
    28741 gtaaaagaga ctcggtagcc tcgtctctct ttatcttact gacctaacct tcttgccaat
    28801 tttcttggtc ttctttaaag cgttttagaa gatcaaggcc ctctggcgtg atgtaacctt
    28861 cttcttgggc tagatggata agctctctat agtttgaaag cgtcacaagt ttaacaccag
    28921 catctgcaaa gttcttatct gcttttggca attggtagct gaaaatcgct acaactccaa
    28981 gtacatctgc tccttctcgc ttggctgctg ctacagcttc aagaactgaa ccacccgttg
    29041 aaataaggtc ttcaaccact accatttttt gaccttgagc tacgcgacct tcgatttgat
    29101 taccagctcc gtggtctttt ggtttgctac ggatgtaggc aaaaggcaag tccatcttat
    29161 cagcaataat ggctccgtgt ggaatccctg ctgttgcagt tcctgcaatc acttctactt
    29221 caggaaaggc ttctttaata gcttccacaa aaccattttc aattagggta cgagtttctg
    29281 gataggctag tgtcacacga ttatcagtgt aaatcggtga cttgatacca gatgcccaag
    29341 tgaagggttc ctctggtttg aggtaaacgg cttggatttt caagaggtgg ctagcgatat
    29401 ctttagcaag tgtcatggta ttctcctttt atttttctaa tctagttctt taattccagt
    29461 tctgtgtcca ttcatccttg atggcatgat aagctgcaac aggatcctca gcttgggtaa
    29521 tgggacgtcc cactacgata tagtcactgc cgatttgata ggcatcagca ggtgtcatga
    29581 ctcgtttttg atctccaact gcaacaccag ctggacgaat ccctggtgtc agacagataa
    29641 aatctggatt ggtagcctgc ttgatgactt gtacttcctg agccgagcaa acaacaccat
    29701 ccaagccagc ttcagctgtc ttcttggcat agtgaatcac agactcttgc agactggttt
    29761 ggatattttg aaactcctgc atctgagctt ctgacgttga tgtgagctga gttacagcga
    29821 tcaatttggc ttgactccca agaccttcac gcgccgcctt catcatctct acaccaccag
    29881 ccgcatggac attagtcata tcgacaccaa gctgagacaa gaccttcatg gctgacttga
    29941 ctgtattagg aatgtcatga agtttgagat ccaaaaagac actatgaccc aaacctttta
    30001 agtaggacac aatctcaggc cccgctgcgt aataaagctc catccctacc ttgagataaa
    30061 ggctttcttc tgctgggaaa agagctaaaa attccttgac cgcctcaaaa ctaggaaaat
    30121 caagagcaat gattggacga tgttctcgca taggtttctc cttttacctt tactagtgag
    30181 agagtctggc caacagaagc cacgaaaaaa ggaacctgcc aacagcaagg ttccacgaaa
    30241 aatgggtagt ctccaccctt tcaccgtcta accttagctg cctctctgga ctgctttaaa
    30301 ggttttctat tattctatta tttctactag cacttgtcaa gtaaaaactt agtcacgtat
    30361 gtgttgactg ctattgaaca cctcacaaga ctctttccaa aagaatagtt aaagactttc
    30421 acttaggaca gagaaaaaga gtttgcttgt taccctttaa ttgaacaaat gggaattctg
    30481 ttttattttg cggatgccta ttcatcttgg caaaggggaa gtaatgagaa tgcgaatgga
    30541 cttcttcgtg agtattttct taaaccgatt tggcaacaat taccgaaaaa atccttaatg
    30601 gtgctttgct agcttattaa tcaccgtccc caaaaatact tggattacaa gacaggtttt
    30661 gaagcactta tagaggaaat ttaaaaatgt tgcatttttt attgcaattt atcagttatc
    30721 ttatcaccca aaacttctgc aattagtatg agcactattg ttatttttct tttttcgtac
    30781 actcaaatca aaatatagca ataagactat tctgtagaat tgctgtcaaa aagatcagtt
    30841 catttttcct tcccacttgt gtttataatc attaaaataa ctcaattaag aaattttctt
    30901 tttcctaata gttatcgtaa ttaaagttaa tagaagaaca atccaaataa gtagtgttag
    30961 attaggaatt agagttttat tccatgaaaa tccctcccat tttgtgaaaa acttagtaaa
    31021 cagtcccata aaactataat ctgttatctt cgcaactgtc ttatccaaac cagatagcat
    31081 cggtaagaaa gcaactaaaa tcatagcagg aagactgata acctgagcta ctacttggct
    31141 cttcgcggtt aaacctatca ataaatagaa taaaatgatg gataaagagg ttagaagaac
    31201 gattgtaata taattaaaag tatgtacaat tgtaactcct aaaataagag gagtagttcc
    31261 cataatcaca aaagttagca aaaaaggaag aaacatcgtt gataaaatgt attcggatcc
    31321 tttaacacca ctcaacagaa gagtttgtaa attgtacttt tctttttctt cagacaagat
    31381 aatagttata ggacttccaa cagccaaagc caaagaaaaa ggtaaacaca tcatcaaaag
    31441 aactaatgcc tgttgatcgt tgaccttccc ctgtgtttcc ataagatatt tataaaaata
    31501 tgtgaaagca aaaggcacta aaacttgcaa taaaatactc ttattggaaa ggataatttg
    31561 acaacgcaac cataccaaag ccagaaaccg tttaagcatt tagctttcct cctgttaatg
    31621 tgataaaaat atcttctaaa gtaggctcac atgaatgaat tgaaaaaata ttttccagat
    31681 cagcctgaga tacctgttct agtgatgtaa aatcaaaagt tatctgattc ccattataat
    31741 ctgtaacctt aatcttttta tctttattat atctttgaat gagttcagaa ggagctcctt
    31801 gctcaactaa tttcccttta tttaataagg caacataatc acataaaaga gttgcttcat
    31861 tcatatcatg agtcgttaga aaaatcgttg tccctgctgt tttcaattct aaaattaact
    31921 catgaattgt tcgagaagtt gtgggatcta gacctgaggt cggttcatcc agaaagagta
    31981 cagcgggttt gttgataaga gctcgtgcta aaagcattcg ttgcctcatt ccagtggata
    32041 attttcctgc taccatcttg cgactatcat ataatcctac tcgctttaac aaattatcaa
    32101 cacgtgattt actaatatta taaaatttac tataaaaaag aagattgtta tacagagaca
    32161 ttttctcata aaatccactt gtatcgctaa ccaatccaat tctctttaat tcaccgcttg
    32221 ttaaattttg agatttttgt cccaaaataa tagattgtcc tttatcggca aggaactgcc
    32281 cagtcagaat attaatcgtt gtggtctttc cagaaccaga tggtcctaaa aatctaacct
    32341 tctttaacag tgaagcttat attatttaaa gctattttat caccaaaact ttttgataaa
    32401 tggctaactt caatcatact cattgcacct cctttatatt ctacattttt acttaacatc
    32461 tatttactta tctttatcaa ataaacgtct taaaaaaaac aataacatta agcatataac
    32521 cgttagcaag attgtttcaa tattattgat aatacgatac attctcaagc agtataaaat
    32581 aattatatat atgcaaatgc tcattatacc tattttttta aacatactaa gcctccttgc
    32641 taaataataa agttatataa taactaagac acactatacc taacataata aatatccact
    32701 ggtaactgaa taaatgttct aaaattaaca agcaaaaata aaatctcaaa cgatgaaaaa
    32761 agccatacta tattttccaa tgatgtttga atatttttat tgtagtaagc ataatatata
    32821 gcaaaaataa tacccaagaa caatactatt aattgtgttt ctataacagt gaactgccca
    32881 atagcagata aaaaagctac tcctcctgat aataagcaaa aacaaactga acgaataaat
    32941 gttttcgctc taatattgtt atctttcact ttattatttt tttacaaatt ggacattctc
    33001 cgttttccag aacatatttt tcttgactta gagcatgatt tctcatatta taaacgtaag
    33061 cgttatctat taggatttca ttataacatc ccagaaatat tttaattatt aaattagtca
    33121 ttatccctcc caaaatagca ggcatctctg gtgtagtcgg ccactttgtc ttatcagata
    33181 aagtgtactt atttatatct ttgtttatat tccgacaacc aaagcaagaa ccgatggtag
    33241 ggatatatat tatcagtaat aagatagcat ccattaaacc ctcctgatat gtaggatatc
    33301 ttatgcgata cagcaaattg attgacaatt tttataatat caatgggcgt atcaattgct
    33361 ttaacgataa aatctatact cccatattcc gcaacaattt tttctaattc ttctactgaa
    33421 gaaactttaa tagaaatagg tactacctga acatctgaat tatacttgtg tattttttca
    33481 gaaagaacat taatcttctc cttaccaaca tccgactctg tataaagaat ttgcctattt
    33541 aaatttgatg gttctattat atcgtaatca gcgacaataa gtttcctcac tccatacata
    33601 cttagactta gagatatata acatcctaag gctcctgctc ctaatactaa tactgtttta
    33661 ttctgtattt tattatactc ttcattataa aataacgaat agtaaccttt agttctagaa
    33721 tatctactat caatatctat ctcatccgaa attaaaattt gttcatttaa tagttcaaat
    33781 gttagtttac tgcctaatac ttcttctatc tctaatagtt ccttacaact attaaataga
    33841 tcgttgataa attcttgcga aatttgaggt tctatagcta tttcccgcat actccgacca
    33901 cgcaaataca ttataccttc ttttttataa gcaaaaacat aactatttac gtagaacata
    33961 tttcgtcctt cctctcatat ataatataga aagcagtata gaaacaacca cccctcctat
    34021 actacgaaca ataatattag gtgtcaaaaa ctgtaatagt tgactcaaag ggaaataaat
    34081 aataacaaat gctattaaaa gttgaactat atattgaaaa ttcaagttat tattgttaga
    34141 attcaagtat cttaaaataa taccactatc atactgaaaa aaaggaattg aattaataaa
    34201 aatcatacct aagttaatga taaaaaataa gagtccaagc aatttatacg atgtgaacaa
    34261 taaaacaagt aaacctgtaa gtgataaaat cgaatttgaa gcaattcctg ctaacataat
    34321 tttcaaaaat tgttttttat tttttaagaa ttgcacccca cttaaatcta caaaaaaaag
    34381 ccaaattaaa caaaagtagc attattccac aatttgggat ctcaatttga taattattag
    34441 caattattgc atgactaaat tcatgtatcg ttgcagacat aataacaaca agcaaccaaa
    34501 ataaaagaat gtttagcgtt attggaaagt tttggaaatt agtcaaaata tccgccctat
    34561 aaatatacat aataaaacat tgcaaaataa gagccaatgg gaaaatacca aataggattt
    34621 tttcaaaatt cttattaaat cttttatagt ttaatttttc agataatgtt atattaaagt
    34681 cccaagatat tttaaaaata tttatctttt ttcgtgattt catagaatct ataagtccca
    34741 ttttactaag aacaatataa aatcttaaat aatatttctt ccctactaac actaataagt
    34801 ccgatatggt aaattcttga cctattagtt gctctaacca tttttttcct gtttcccaac
    34861 taatacatgt ttcttactag atggtatata tagatgatac tgtccctcat aaggagtagg
    34921 taatatctta atcttatcaa caattctcat aacaaaccta acctttaata aataaaagag
    34981 gggctaagta aaacatactc tagtccctca gtccgcaatc aataaaataa tgactaattc
    35041 tattaatctc ttgctgcaag actaatacgg aaaattcaaa tgactgtcta aaatgaaatt
    35101 gaaaaagctt gatgttagta aatgaacaaa cttcaacaac cagcaagagc aaggattgct
    35161 aaaccaaatc cgatacctgt tgcaagtcca gctgaaaaat cactaactgg tactgaaatt
    35221 tcttcaattt cctcaaataa aaagttatct tgcatgatta tcactcccta tctttttaac
    35281 aacaggctat tccaattaag agaactaccc cagttccaag taaaactccc ccaaaaattc
    35341 accatctgta ggggctacga tttcttcaat ttcttcaaat aagaatgctt catctgacat
    35401 atgtgttacc ttcttcataa aaaattattt tgtaatcgat tacattgcaa atgtaacata
    35461 aagaaaaaca gatgtcaaat attaaacgta agaacatggt cactaaagaa ctataagaga
    35521 aaaggtaaac ctagcgacgc gatgagcgct gggtcgtttg atttcgattg ctctcttcct
    35581 cttgtttttt ctgttcttct tcctgttttt tctcggcttc cttggcctct tgtttggctt
    35641 tttcctcagc ctccataatt aatttatccg ccacagtgta actgtaaatt ccagcttcca
    35701 tgtcgaccac actcggttct gacaattgtg gcttaatctt actgtaatat ggcagtttct
    35761 tactcatttc agatagagga accaagactt cgtccgaatc attcatggtc aatcgaatta
    35821 aatcggatgt caccttgctt ggggctaatt ccaccttttg gatagccgcc ttgagttctg
    35881 ggctaatttg agcaagttct gagacaaaaa ccttgatttg ttcactatca ttaaagagaa
    35941 ctgataaata agtttctggt aaactgttca gactcacaga actagtctca agctgaccac
    36001 tggaaagaat aggataatga ttttcaccag aaatatagta ggccacaata tcatattcct
    36061 tgaccttaat agtgaactta gttggaaatt gatagacaag ttgagctgat tcaacccaat
    36121 agttagactt aatctgcttt tcatattttg ccttgtctag cagaaggtta atcgtataat
    36181 ccgaatcctg aatgcctgaa gcctgtcgaa tatcatcagc tgtagtttgc accgttccct
    36241 caacacgaat atctttcatg gtcgcataag gactgagcaa gtaggcagag acaatcaata
    36301 aaagcagact tggaaataaa atcgtgaagg ctcgcaagat atggatacca ggaatctttg
    36361 ctttggctgg tttttccttt gtagcctttt tagaaagctt tttatcctgt tcctccttct
    36421 ctttagactc tggttcttct ttctcttctt tctctttgtc agcctctgag gatgctactt
    36481 tttcttcaga ctcttcctta gctgattctg aatcttcctg gtctgtttca ctctcctggt
    36541 cctgtttgtc ctctgacttc tcagattctt ctcccattcg agcttgtctt tccttttcct
    36601 tctcctcagc tagagccgcc tcttcttcag ccttcttttt tagatattct tggtttcgtt
    36661 tctgccattc tgataactct ttcaattctt cgaggatttc tttgtcctca tttttcttat
    36721 cttttgacat ttactttcct tatgataaat cttttttcaa caattgataa aaatctgcta
    36781 gagatttcaa ttccttagaa gccttcatct tagcttggta atcttccttg tgacttagta
    36841 agtgagaaag cttctcttcc aaactatcca aggtcaaatc gctttcttga aggtcttctg
    36901 catagccttt ttttacaaag taagctgcat tttcaagctg gtcaccacga ctagcttcac
    36961 gaccaagcgg cacaatgaca tgcaattttg ctatcgccaa gagctcaaaa atcgtattgg
    37021 caccacctcg tgtcacaaca atatcagcca attccatcaa gggttgatag agatcggtca
    37081 catagtcaac acgaaaaaga ttttggctca actcattcag gctagaatct ccagttagat
    37141 tgataatatt gtagcgctct gttagttctt tcttatggtc tgtcaccaat tggttaaaga
    37201 cacgagcacc tgcagaaccg ccaacaaaca atacagtcgg caatttatga ttaaagtggc
    37261 tttgaatatc caccaattca tctggttctg gatttttttg atctgaaacc ttggccactg
    37321 ctcccacatg ctcaacctta gacaaactcg aagcttgctc aaaggttgaa tacatcttag
    37381 tcgcaaattt ataggcgatt ttattggcca agcccataga caggtcagat tcgtgaataa
    37441 agacaggcac tcctgacaca cgcgcagcga taacaggcgg tactgagaca aagcccccct
    37501 ttgaaaaaag ggtctgtgga cgaagtcgca acatgataaa gagcgattgg acgattcccc
    37561 agcaaacttt gaagacgtcc agcatatttt gccaagagaa atagcgacgc aattttccag
    37621 tcgcaataga atggaaggtg acatccaaac ctgacttaag gatttcttgg tgttcgatac
    37681 cacgcttgtc cccgatatag tggacttccc aaccatcttc gatgaacttg ggcattaaca
    37741 aaagattgag ggtaacgtgt ccaaccgtcc ccccacctgt aaagacaatt tttttcatat
    37801 tattctttta actccgctac tgtgtcgata aagaggtcgc cacgtacttc aaagttagca
    37861 tacatatccc agctggcatt ggcaggacta agaagaacca catctccttg agtcgcaagc
    37921 tcataggcct tgcgggtcgc atctgcaata tctgtcgcct ccacataagc gacaccagcc
    37981 ttgtctgctg cccgtttgac acgttctgca gattgaccca ggatgaccat cttcttgagt
    38041 ccagtaatgt ctggcaccaa ttcgtcaaac tcattgccac ggtccaaacc acctgcaatc
    38101 aagacgacct tgctgttatc aaatcctgac aaggcttttt gagtagccaa gatattagtt
    38161 gatttactgt cgttatagaa tttaacaccc ttgatgtcat ccacaaactg gagacggtgt
    38221 ttgacaccac cgaaggctga aagagtttcc ttgatggttt gattgtccac accacgaagc
    38281 ttggctacag caatagtcgc aagggcattt tccacattgt ggctacctgg aacaccgatt
    38341 tcattcgctg ccatgactac ttcaccacgg aagtagagtt gaccatcttc cagataagct
    38401 ccatcaacct tttcaagtgt tgaaaatggt acaacagtgg cttctgtctt ggaagtcaag
    38461 tcttttgcca agtcttgatt aaagttcaag acaaggaaat cagctgctgt catcttgttc
    38521 tggatattcc acttggctgc tacatattcc gaaaatgacc catggtagtc gatatgagtt
    38581 ggcatgaggt tggtaataac cgcaatctct ggatggaatt cttgaacacc catgagttgg
    38641 aaagaagaaa gttccataac aagcgtgtcc ttatctgatg ctatttgagc aacctgacta
    38701 gctggatagc cgatattccc tgataaaaga ccatgttgcc cagcagcagt caaaacttcc
    38761 ccaatcatag tcgttgtggt tgtcttaccg ttcgatcctg tgataccaat aatcggtgct
    38821 tctgaaatca aataagccaa ttccacctca gtcaagactg gaattccctt ggccaaagcc
    38881 ttttcaatca tgggattgtt gtaggggata cctggatttt tcaccataag ggcaaactct
    38941 tcatccaaga gttccaaagg atggccacct gtaatgacct tgatcccttc ttccagcaaa
    39001 ctttgggcag ctggattgtc ctcgaaaggt ttcccatcat ttactgtcac aatggcacct
    39061 agcttgtcca acaaacgagc tgcagattca ccagacttgg ccaaacctaa aacaaggact
    39121 ttcttatttt taaattgatc tattactttc atgtctcgaa ctccatttct actcctacta
    39181 ttttaccatt tttatggaaa taaaaaagcc acaaagtgtg tttgtgactc tttcttctaa
    39241 ctgaatctta ccatatcatc tatgtgataa atcggtaact cgaatgacct gatccacttg
    39301 ttcccaaata agaggattgt gggtcgcaat aataatggtc cgattcggat tttttaaaga
    39361 ttctaggatg gaaagtaatt cctcagagtt tttggggtct aaggaagtgg ttggttcatc
    39421 tgcgaggatc aaaggtggat cctttaaaat tatcttcgct aatgcaacac gttgtgcttc
    39481 tcctcctgat aactcaaata taggttgctt caaatccaaa taagagaggt ttacacagtt
    39541 tagagcttgt ttcatcaaag agattttctc tttttccttc aactttttac caactaaacc
    39601 cagattgaga ttctctttga cggtttggct ttcaattaag ccaaaatctt gaaataagta
    39661 tcccaagtaa tctctaaaga aaacagaagg cttgatgtcc ttaagagaag taccatcata
    39721 gatgatttgt cctttgtcat atggctctaa tcgtccaatc atattcaaga atgttgtctt
    39781 accacagcca cttgtaccga ttaaggcata aattttccca ccttcaaaat gaagatttgt
    39841 atctgaaaat agctgacggc ttccaaattt tttagatata ttctttagtt caatcatcct
    39901 attttccttt cataattgtc atagaaacac gagattcttt ctgcgcttga cggtaaagcg
    39961 tcagaactgc actagctaga aagactaata aagtgagcaa gccaatcacc aagtctcgac
    40021 tgcttaaaat aaagagacta gcaccaaata caaaactggc aaattgacta accatatact
    40081 gagcatgtgt ttcaaaaaat cgtaaacctg aaattcgttt aatcaaaata tctcggcgga
    40141 attgctcgaa atatagaaga ttgacagaat aaaatagtaa taaggaacta gctatcccca
    40201 caatagctcc taagattaaa gttgctgttt cagtttgaac ttcattataa cgagttagat
    40261 aaacacttct tccttcttta agataggata cttgctcata aattccagct ttcttcaaga
    40321 gttctagccc actctcatat cctttgataa agagttgttt tccagcattg atagaccaac
    40381 tagataagga tataaaacta tcacctgtag aagtcggcgt gaataccact aaaatcggat
    40441 cagtcaaata ctgagtagat acgggattct caccgttatt ataaacaaac cgcttttctc
    40501 ccattgaaag ataactaacg tgcgctttca tctcataatc caaaggagca cttgcctcct
    40561 caccagattt tccataataa ctcaatcttt cttcaaaaac tttcttaagt tctgcttctc
    40621 gagagcgcaa atgttctggg agcaagagga taaactcacc tttttggaga tgggctaact
    40681 tctgtttggt ctcagcatct accacgacct tttccttgtc caaataactg ggactaacat
    40741 agagcgtatt agcatctgga ctataggtat ccagtgtctc tccctgttca ttttttcctt
    40801 gtggattggc aaaatggagc agattatcct ttacataaag agcttgttct tcttcgattg
    40861 cttccttggc aaaggcatac cacttgctct gattttctgt atcttttcct ctatcaccta
    40921 agccaaaaga aatttgataa tagtctgctc tatcctgcca tgcttgtttt gaaatttcaa
    40981 gttctttcaa tcgttggtaa gacatcaaac ctgtcttaac agcgtagcct actgtaaaga
    41041 cagcaaccaa ctgacacaat agggttaaaa tcatcaggcg tttaaggggt aatctcccct
    41101 taataacggg aactaatgct ttgtaactca aactcattag ataaagaagc attagtaaaa
    41161 ttgaaatcgc caataaaaac aacagataga aactaatccc aaaaccatag gtggctaaca
    41221 agataggata aaacaaacct tgactaaaaa gaacgactcc cccacctagg aaggaaagga
    41281 gggctgatag aaggagccat ttgatatcag tagataaaga atgccccatg atggataaga
    41341 gagtctgacc agaaaagagt tttatacctg ctgctctcat ttccttaatc cgagtgataa
    41401 tcactaaagc aaagaaagat aagccaaata ttgctaaact aattaaaata aggggattta
    41461 gtaatattcg aaaagcaaga aaatagggcg gtatctttcg gtcagcactt gctttataac
    41521 ccaaatctcc taatttatcg gcaagctttt ctttcgtcaa ggagcctgac aaaaggagat
    41581 aactatttag cggatcacta cgttcacgac tttcttggct agcttcttgg aattcttttg
    41641 gtaaagttcc ctgaccataa gttgcataag taaagtgagt cgtcccatcc ttactcggct
    41701 ctacaattct tctagctatt aaactctgtt ctgagtttgc aaaattctcc aattcctgtt
    41761 caaatacctc acgcgtcggt tcctgagtat cttttttgac acgaagtaaa gaaacggaat
    41821 catagcttgc atataaatat tgtggcgcac gtaagacaat aatccaagca aggaagaagc
    41881 tgagaaaaaa agttgataat aatatgaata gtttcttcat agtagactcc ttgtaaacaa
    41941 aattccccct gtaatttctt acaaggggaa cgatttaaat caatgaacga ttagtcataa
    42001 tcacagtaaa atgctacttg ttctccccat ttagtccaaa tccatgcata ggaggttttt
    42061 ccagcagaag cttctccttt atctgaatga ctattccatc tacttacaag agaagaataa
    42121 tgatattttt taccatgata gtaatttgaa aaagcccaac cacctcctga accttctcca
    42181 tacgtccata ctcctccatc tggatattgt acagcagctg atgcagctcc caataatgta
    42241 aaacttgaaa taagagctag agcaagtaat ctatgttttt tcgttttcat tttatttttt
    42301 ctttcaaaaa aagcacacct tgagcaacaa tgcaacaaaa taaaccctcc tctctctttt
    42361 gttgaaaccg ctttcttatg tgataaaaat atcattttta ttatttgttg tcaaggaaaa
    42421 aatcgaattt tttagatatt ttactacatt gcctctatga ataatattat atagtagttt
    42481 tattttaaaa taatatgcaa ccagtactaa ccaaatataa aatagatgcc attaacgaat
    42541 tttattcaag tttttcccat tcatactatc caagcaaaag agatgatgtt atagtacaaa
    42601 acaattcaaa ctattgtaaa attcctagca aaaaagagag ccaaaactct cttttttatc
    42661 ttcttttact ttttttgact ggcatgagtg taatgtctct aacactaaag taagctagga
    42721 tcaacatggc tattgctagg aatatttctg ttggtaattg aaaaattttc agaaaagata
    42781 gaaccaataa aatcaagagt gccactaaaa tacataccat agcgacgata ttgactgtcc
    42841 ctttaatgct ttctggtgtc gcaaatacat agagtaggag cagtaaaatt cctaggacta
    42901 aatagaccat ctttctctct ttctagctct tattcagctg attttttctt cttgttagct
    42961 ttctcacgct ctgctttgtt aaggatttgt ttacgcaaac ggatagactc aggcgttact
    43021 tccatgtact catcgtcgtt caagaactca agagactctt caagtgtcaa gatacgaggc
    43081 gtcttgataa cagctgtttg gtccttagta gctgaacgaa cgttggtcat ttgttttgcc
    43141 ttcgtgatgt taactgtcaa gtcattttca cgagagtttt caccgatgat cattccttca
    43201 taaacctcag tacctgggtt gacaaagatc gtaccacgtt cttcgataga catgattgag
    43261 taagttgtag ccttaccagc atcgatagaa acaagggcac cacggtgacg tccaccaatt
    43321 tcccctggaa tcaatggcaa gtattggtcg aaggtatggt tcatgatacc gtaaccacga
    43381 gtcattgaca agaactcagt tgagtatcca atcaaaccac gcgctggaac aaggaagacc
    43441 aaacgagttt gaccattacc agttgaaatc atatccaaca tttcaccttt acgttcagaa
    43501 aggctttgga taacagaccc ttggtattct tctggagtgt cgatttgtac acgttcaaat
    43561 ggttcacatt taacaccgtc gatttctttt acgataactt ctggacgaga tacttgaagt
    43621 tcatagccct cacgacgcat tgtttcgata aggattgaca agtgcaattc tccacgtcct
    43681 gaaacagtcc atttatctgg tgaatcagtt gggtcaacac gaagggaaac gtctgtttgc
    43741 aattctgcct gcaagcgttc ttccacctta cgagaagtta cccatttacc ttctttacca
    43801 gcaaatggtg agttgttgac caagaaagtc atttgaagag ttggctcatc gatgtgtagg
    43861 attggaagag cttctactgc atctgtcgga gtgatggttt caccgacaaa gatgtcttcc
    43921 atacctgaaa cggcaatcaa gtcacccgct ttggcttctt ggatttcacg acgttccaaa
    43981 ccaaagaaac cgaagagttt tgtaacacgg aagtttttag ttgtaccgtc aagtttagaa
    44041 agggtaactt ggtccccaac cttaactgta ccacggaaga cacgaccgat accgatacgt
    44101 ccaacgaagt cattgtagtc caaaagtgac acttggaact gcaaaggctc atctgagtta
    44161 tctactggag ctgggatatg gtcgataatc gtgtcaaaga ttggtgccat agtcgcttct
    44221 tggtcagctg gatcatctga caatgaagaa gttccgttga tcgctgaagc ataaaccact
    44281 gggaaatcaa gctggtcgtc atctgcacca agctcgatga aaagttccaa gacttcatcc
    44341 actacttctg ctggacgagc tgatggctta tcgattttgt taacaaccac gattgggaca
    44401 aggtcttgtt ccaaggcttt tttcaatacg aaacgagttt gtggcatggt tccttcatag
    44461 gcatctacga ccaagacaac accgtcaacc attttcatga tacgctcaac ttctccacca
    44521 aagtccgcgt gtcctggtgt gtccataatg ttgatacgag ttccgttgta agcaacggca
    44581 gtatttttag caaggatggt aatcccacgc tctttttcga tatcgtttga gtccatagca
    44641 cgctctgcca attcagtacg tgcatcaagc gtttctgatt gtttcaataa ttcgtcaacc
    44701 agggttgttt taccgtggtc aacgtgggcg ataatcgcaa tgttacggat atcttctctt
    44761 aattttgtca tgatttcctc tataatattc aaaatttatt ttctaactga acgattatac
    44821 cataatttca aataaataac ataactcaag caagtgtaaa tgttttcact ccgcttttct
    44881 tttcacgtca agccttttca aagcaagcga cttatgataa gataggcaca gtatgcgttt
    44941 agataattta ttagcccaag aaaaagttag ccgaaaggcc atgaagcaag cactcctcag
    45001 aggggaaatt ctagtcgatg gttgcccagc ccgatcccta gctcaaaata tcgatacagg
    45061 actacaagaa ctcctttttc aggatcgaat cattcaaggc tatgaacaca cctatcttat
    45121 gcttcataag cctgctggtg tcgttacagc caacaaagac aaggaacttc cgaccgtcat
    45181 ggacctgctt ccatctgaca tccagtctga caagctctat gccgttggcc gactggaccg
    45241 agatacaacg ggactcctcc tcttgaccga taacggtccc ttgggctttc agctcctcca
    45301 tccccaatat catgtcgata agacttacca agtccaggtt aatgaacttc taacacctga
    45361 ccatatccaa gcctttcaaa agggaattgt ctttttagat ggtactgtct gtaaacccgc
    45421 aaaactagag attctatctg caagtccctc cctcagtcaa gcctctatca ccatttcaga
    45481 aggaaaattt catcaaatca agaaaatgtt cctctcggtt ggtgttaagg tgactagcct
    45541 caaaagaatc caatttgggg acttcacatt gaacccagat ttagcagaaa gtaactaccg
    45601 ccctttgaac caaaaagagt tacaaatcat taaaaactat ttagagatga gtcgataaaa
    45661 caaaaaaagc tttaaaacta aagctttttt cttttattta ccgaaaaatt aaggcgattg
    45721 ctacaatcca gttaactaca gaaatcacaa ttcctaagat attaagaatc ttttctattt
    45781 tatagtctaa ttgtgactct ttttggtatg aaatagccaa gaccaatcct atgataccca
    45841 aaatcaagcc tacaattgga aataacaaac caagaataat cgacaagata cccacaaaaa
    45901 gtggattttt cttcttttct ttcatgttct aagaactctt taaattttat acaaattaat
    45961 tatactataa aacaatagct tcatcctatc attcgactaa tttggaaata aggttagcta
    46021 gtcttcactt tccctttcca agaatccaag ccataagaaa ggatataaat ctcagaaaaa
    46081 ccttgttttt tcaagtaaag agctgcattt gtaactcgtt gcgcacgttg gttctcgtag
    46141 agaaggacag gtttatcttt acgaagggct gcaagactag ttttcaactg acttgaagga
    46201 atattgcgtg caccaaggat atgttttctg tggaattctg ctgggtcgcg caaatcaatc
    46261 aattgacccg tacgaatcaa ggcttcaaac tcctcattgt ccacaatttt agccgcacga
    46321 cgaatacgaa gatagttaaa gcccatccac gccaacattg ctagtataag tgcccacaaa
    46381 atccaagtaa ccattagttc ttttctccat ttttctcaat ataatccaat tctaccttgt
    46441 gctctctgcg aagaactgct tctgcctcta gatagtctaa tttatccatc aaccctgcat
    46501 cgtaaatccg agatagttcc aacttcatca gttcaatatc atataagcgt tttcccatgt
    46561 aaacaataat accaaatcgt ttgaggaatt gctgcacatc atagaatgtt ttcataagac
    46621 tcattctagc aaaattttgt gtttttttca agaagagact cacacaatgc tccttatttt
    46681 cctatcttct ttggcgattc taaggcaagt gtggtacaat aaaaacatga gaattcaaca
    46741 attacattat attatcaaaa tcgtcgaaac tggctctatg aatgaggcag ccaagcaact
    46801 ctttatcact cagccaagtc tttccaatgc tgtgagagat ttggaaaatg aaatgggcat
    46861 tgagatcttt atccgcaatc ccaagggaat caccttgacc cgtgatggga tggagtttct
    46921 ctcttatgcc cgtcaggttg tcgagcaaac tcagcttctg gaggaacgct ataaaaatcc
    46981 tgtcgcccac cgcgaactct ttagcgtttc gtctcaacac tatgcctttg tggtcaatgc
    47041 ctttgtctct ttgctcaaga aaagcgatat ggagaaatac gagcttttcc ttcgtgaaac
    47101 tcggacttgg gagattatcg acgacgtcaa gaacttccgc agtgaggtcg gggtcctctt
    47161 cttaaacagt tacaaccgtg atgttttaac caagatgctg gatgacaatc acctactagc
    47221 ccaccatctc ttcacagcgc aaccgcatat ctttgtcagc aagaccaacc ctctggcaaa
    47281 gaaagacaag gtgaaactgt ctgatttgga gaatttccct tacctcagct atgaccaagg
    47341 gacgcacaac tccttctact tttcagaaga gattctttct caagaacacc acaagaaatc
    47401 cattgtggtc agtgaccgtg ccaccctctt taatctcttg attggtttgg atggttatac
    47461 cattgcgaca gggattttga acagcaacct aaacggagac aatatcgttt ctatcccact
    47521 ggatattgat gacccgattg agctggtcta tatccagcat gagaaaacca gcctatctaa
    47581 gatgggcgaa cgctttatcg actatctact agaagaagtt cagtttgata gttgagaaat
    47641 gataagaacc aatatgtagg ctagcaacaa cctgcacatt ggttcttttt acttataatt
    47701 aaaagtttcc cctgccaact tatcagctag cttgggaaag agagtataaa acttatgggc
    47761 taggttcaac aaaatcggga gattgagttc tcgtttattt tttcctataa tcttgacaat
    47821 ctttttagcc actgcatctg cctctaagag gaagcggtca accgatttaa gataagttcc
    47881 atctgggtca gcttggtcaa aaaatcctgt tcggattgga cctggattga ctgttgtcac
    47941 atagactcca tagggcataa gttcgagtcg cagagcattt gaaaaaccaa tagccgcaaa
    48001 cttggtcgct gagtaaagac tagacttgcc agtagctatt agacctgcca tgctgacgat
    48061 gttgatgata tgccctttgc tgctttcctt catacgagcc gcaaggtgac gagacagatt
    48121 catcagggca aaggtattga cctcaaacat ctggtgaata tctttatcag caatctggtc
    48181 aaatccctca aaaatcccgt aaccagcgtt gttaatcaag acatcaatct tgccatagcg
    48241 gagataaaga tcagttacca gagcttctag ggctgaatcg tcggtaatat caatttcaat
    48301 caattctgca tgggaataat ttccgtagag ttgggctaat ttttccttat ttctaccaag
    48361 caagatgagt tggtcattgg gcaggagttt gaccatttct tgagctagac caccgctagc
    48421 tccggtaatg agaatagtag gcatacttat cctttctgtg actgctagat ttccacttct
    48481 tccaagtctt tgaccacatg gacattttca aaaattgtgg cagcgtcctt cttgagtttg
    48541 cttatatctt ttgagaggaa acgggcactg atatggttga gtaggaggcg tttggcacct
    48601 gcttctaccg ctacttgtgc agcttgcata ttagttgagt gaccatggtt acgagcaatt
    48661 ttttcatcac ccttgccata agtggactca tgaactagga catctgcatt gacagccaga
    48721 cgcacactgg catccgtttt tcgagtgtct cctaaaatag tgataatctt acctggacgt
    48781 ggcgctgaga tatagtctgc tgccttgatt tcagttccgt cttccaaaac aagatcctgg
    48841 ccgtttttga ttttaccaaa aagcgggccg aacggaacac cagcagcctt gagtttttca
    48901 gcatccagcg tcccttctag atccttttgc atgacacgat agccaacaca gaaaatagtg
    48961 tggtccagct cctctgcata cacagtgaat ttatcggttt caagaatttt acccagagaa
    49021 tcttggtcaa actcatggaa atgaatgcgg tagggcagac gagaacctga cacacgaagg
    49081 ctggttaaga caaatgactt gattccttga ggtccgtaga tttccaaatc tgtctgctct
    49141 tcattggcct gaaaggcacg gctagaaagg aaacctggca aaccaaaaat gtggtctcca
    49201 tgcagatgag taataaagat tttgctgacc ttacgtggtc gaattgtggt ttccagaatg
    49261 cgattttgcg taccttctcc acagtcaaag agccaaactt cgttaatctc gtccaaaagt
    49321 ttcagggcga gacttgaaac gttgcgggct ttagagggct gaccagcccc cgttcctaaa
    49381 aattgaatat ccattcgata ctttctaatt aatcaatata taacatggct gtgcggtttt
    49441 ccgatcggaa atagcgtttg ccagaaaaag cagcagcttc ttgcaataaa tcctcttggc
    49501 tgtagccttt gagacgtttt cgaccatcag ccaatctttc caaatcagtc aaagctgtga
    49561 gactttctag gctgacaact tcctcgtcct tgacaggctt catgtaaatc ttaccagact
    49621 cttcaaagac taattgatgg gagaaaattt gcgcaatttc aaagagcaag tcatccgaga
    49681 ttttctcctc attttcagag aaaatccgac caaagccgtc actctcataa caaaaaccaa
    49741 aggatttacc agacagatta agccgaataa aaggcttatt ttctagggtg aaacttggct
    49801 cagtattgta aagattcagt tcctgactga gttctgcaaa ataatccgtc gcagcctgag
    49861 gacttttttt ctgatagagt tctgcaaagt aggcattaac aacacttggc ggaggtgtaa
    49921 taagtgttaa ctgctcctgc tctgttttac cagctagaag ctgatccaga tagaccttgt
    49981 ccagacttgt ataacctcca tactttagag ccaaagtttt aatatcagtc ataaaattct
    50041 tctaacctcc atttattttt ctcggaaatg tagcctgtaa tcacttcgcc gtcttcctga
    50101 taatcacgtt cttccagaat tgcaacactc tctaaatcat gaatcttgta ggactttgaa
    50161 aaaggcactc gcagggtaaa tgcttcaaaa atttccttaa tcttatctag caataatgct
    50221 tgcaagtttt cacgactgtc ctcagacttg gcagaaatga gggtatatgg cgtttgggta
    50281 ggcgtgaaat cctccaccaa atccgcttta ttataaagcg tcaagtgagg aatatcttcc
    50341 atgtccaggt ctttcatgat ggagagaacc gttttttcat gctcctcgtg gtaaggattg
    50401 ctagcatcga taacatgaac cagaaggtcc acatgcttgc tttcttccaa ggttgacttg
    50461 aaactggaca ccaactctgt cggcaaatct tggataaagc caacagtatc tgtcaaagtt
    50521 acttggagat tgcctcccag atgaatactc ttggttgtcg catccagagt cgcaaagagc
    50581 tcatctgctt catactgggt cttactggtc aagatgttca tgatagttga tttcccagca
    50641 ttagtataac caatcaaacc aatcttaaaa gtgctagact ccaaacgttt ttctctgaca
    50701 gtcgcacgat ttttctcaac caccttaagc tggcgctcga tatccgtgat ttgattgcga
    50761 acgctacgac ggttcagctc cagttggctt tcaccaggac cacgggaacc aattccccct
    50821 gcctgacggc tgagcataat cccctgacca accaagcgag gcaagaggta tttgagttgg
    50881 gctaggtgga cttggagctt cccttcatgg cttcgagccc gcatggcaaa gatatccaaa
    50941 atcaactgca tacggtcaat gaccttaaca ccgagaactt cctctagatt gacattctgc
    51001 cttggggtca gacggttgtt gacgatgaca gtagtgattt cttctgcatc caccataagc
    51061 gcaatctctt ccaacttacc agagccgacg aaggtcttgg aatcatattt ttcacgtttt
    51121 tgtctgtagc tatctacaac gactgcccct gccgttttcg ctaaactagc caattcttcc
    51181 atggagaggt caaaactgtc cataccctgc aattccacac caatcagcag gactcgctcc
    51241 tcttttttct ccgtttcaat catctaaaaa ctcctctatc tggcttaaaa tgcggtcttg
    51301 tacaccagat tctccaatct gataaaaggt gacctgcatg cgattacgga accaggtcag
    51361 ctgacgcttg gcaaaacgac gggtcgcctg tttaagactc tcacgagctt cctccaaggt
    51421 ctgctctcca cggaaataag gaaagagttc cttatagcca attcctttag cagcctgtac
    51481 attaggggaa tggtcaaaca gccacttggc ctcatccaaa agcccagcct caaacatcaa
    51541 atccactcgg tggttgatac gctcataaag ttgactacgt tcatcatcca agcagataat
    51601 cagcggttca tacaaaatct cttgattttc caaatcctga ccaaaatggg caatttctaa
    51661 ggcacgcata gcacgacgac gattaaactg gggaatctca aggcctgctt gctccaccaa
    51721 atgggctaat tcctcatctg aatatggctc caaactagct cgataagcta aaatctcctc
    51781 atgaggagtc tccccaccta ggtggtaacc ttctagcaag ctctggatat aaagtccagt
    51841 cccaccggcg ataatggcta gcttgccacg gctgtgaata tcctcaatag tcatcttagc
    51901 ttctgaaaca aaatcaaaag ccgagtaaga ctcagttatc tctctaacat cgattaaatg
    51961 atgaggaaca gctgcctgct cttctggact agccttggcc gtcccaatat caagtcctcg
    52021 atagacttgc tggctatctc cactaaccac ttcgccatta aagcactttg caacttcaat
    52081 agctagagcc gtctttccaa cagcagtcgg tccaacaatc acaattattt ttgttttcat
    52141 cttttttcct tgaaaaattc ccattttttc gttactatta ttataacaca aaaaggtcag
    52201 tcagaaaaat gtgacctctt gaggaggctg gctgattgag aatataaagg aggaagactc
    52261 atggctaaag gattcgctaa aggtcttgta acaggtgtcg caggaactgt cgctgccgtt
    52321 gcaggtgcag tatacgcatt taaaaagaaa gtaatcgaac cagaagagca aaaagcagct
    52381 ttcatcgaag aaaaccgtaa aaaagcagct cgtcgccgcg tatcacgtta agaaacaaaa
    52441 gaagttggga ttattctcaa cttctttttt ctattctttt aaatagctag gtcaaacttc
    52501 aactgtggct taacagggtc ataatccacc aactcaaaat cttctgcttt gatatcaaag
    52561 aaattagtcc catcaggaac atttaaaacc aagcgtggtt ggcagtttga cggctcccga
    52621 cggagcaatt cctgagcttg ttcaaattga ttatcataga tatggaggtt gttgatgaag
    52681 tagaagaact tcccaacctt ccagccaaaa tgtttagcaa tcatcatctg caaagccaca
    52741 tactgcatag cgttgatgtg gtgggccacc agcatatcat tggagcgctg ggtcaaggtc
    52801 gcatccagat agatttcccc atcaacacgc cgaacatcaa acatggtctg aaaggcgcac
    52861 gggagcagcc catctgtttc ttcgaaagct tggtaatccc agagagaaat aatattgcgg
    52921 cggttccaag gattggtttc caactgtttg agaagcttat tgataatgtc gtgtttctta
    52981 acaacggcac catagcgctc accaatggtt cccgtgtctc caacttccca gtcattccag
    53041 tagtgaacat tgtacttgtc attaagcact tctaagctat ttgactggtc ttggtaaatc
    53101 cagagaactt ctttgatggc ggatttgatt gcaatgggac gcaaggttat gataggaaat
    53161 tctcctttag ataagtcgta ctcggaaaag gcaccagtta catacttgga attagcaaca
    53221 gtcccatcct tgtacttagg acgagcctgc tctgagaaaa caccttcctt tagaatgcgt
    53281 tcaatattct ctttaaaaat cgtatctgct tttgtcattt actctcacct cattcattgt
    53341 cctaactagt atagcataaa aaataaaaga ggaacattac taactctagg ttagcatttt
    53401 cctctctcat tatcttattg caatacaagt gatgctgctc cgataactcc agcgtcattt
    53461 cctagagttg caagagctaa tttagtagat gtgcgtactt gtgggaaagt attttcgtca
    53521 tagacttttt gaacaccttg tagaaggaat tctcccgcag ctgacacacc accaccgatg
    53581 acgattgttg atgggtttag gattgaacca atgttagcac aagcgattcc caagtaacgt
    53641 gagaagttac ggtaaacaat caaagcaagg tcgtctcctt cttttgcgag atcaaagaca
    53701 gtcttagcag ttacttcttc tccgttatca atcaagcgtt tcaaggctgc atcgccttcg
    53761 tattcatcgg catagcgacg agtcaagttg acaattcctg ttgcagaagc gactgtctca
    53821 agacagcctt tcttaccgca agtacatgag attggctggt caaagtcaac agtgatgtga
    53881 cccaactcac ctgctgcacc agcaacaccg tgaagcaatt tgccttctgc gacgataccg
    53941 ccaccaacac cagtaccgag tgtcataaag acaacatctg gttggttatc accagcaccc
    54001 atccagcgct caccaagagc tgctacgttg gcatcattat cgatgaaaaa tggaatgccc
    54061 aaagcttttt caatcttttg tttgattggt tgaagggttt tccagttcaa gttgtaggca
    54121 ccgataacag tccctttgtc acggtcaacc acacctggtg atcccattcc aatgccttgg
    54181 aagtccgctg ctgccaatcc aagcaagtcc aaacgatgtt gaatagactc aatcatatca
    54241 tcaacgatat gacttccctc atccaaaatg ttggtcttga ttgaccattt tccttggatt
    54301 tctcctgctg ttgttaagat tgcaaatttg atagaagttc caccaaggtc aatcccaata
    54361 atcttttgac tcattatatt ctcctttttc attgtgtagt aattgaaaat gcttacagta
    54421 ctagtatatc aaaatccatt cacttatgca aaggtttgcg taaatgaatt ataaggaatt
    54481 ttttcgccta ccagtatctg ttaaccatct aaatgatcaa tggcatattg agctgcatca
    54541 tttgaaaatt tatcaaaatc agaagttaat tgtctataca tacgtttttt tgacatgtgg
    54601 aataacgaat tataactttt tgcctttcct aaagcagcag aatattcact attgctatca
    54661 ttactagaag aagctgtact tgcttgacct tccttccaaa ctccgtcagc gccaacataa
    54721 taacgtccac catcaaccca ttcattgaca gccattttac catccgattt tagatagtaa
    54781 tttccttgcc atgtactata agcataagaa ccatctgatg tcaaataata ccaagcttga
    54841 taggtggcat cataaaccca ttcaccttta gccattttac catctgattt caaatagtag
    54901 tttccttgcc atgcattgcg agcatatgaa ccatctgatt tcaagtaata ccatgcttgg
    54961 taagaagaat cataaaccca ctcaccttgt gccattttac cgtttgattt aaggtaataa
    55021 gctccttgcc atgcattttt tgcataagaa ccatctgatt tcaaataata ccaagcttga
    55081 taagaagagt cataaatcca ttcactctgt gccattttgc catcagcttt caggtaatag
    55141 tttccctgcc atgcattttt cactagattt ccatttccat caaaatagta ccaagaaccg
    55201 tcctgcttaa cccaaccact tgctgtggat gaagttgtat cagtttgagt tgtagccgct
    55261 tgttttgctt taaaagcacc cttaggagca tttttcaatt cgcaagccac accatcatga
    55321 tcgcggtcta acttggcaga ataaccaggt tctccctcat gaagatccga atatccatta
    55381 gcccaagctt ccttacagct cgaaaaatgg atgttttctt ccgccaaaac tggttgtgaa
    55441 aacaaggcta aaattggcaa cgtcaccaga ctcattttta aaaatagacg tttattcatt
    55501 tctttctccc ataattatga tatcgatttc aatattattt tatcaaactt atttcagcaa
    55561 ttcaaggaaa cttctggtct tttctacaaa ctcctttggt tttgccttat tcaagacatg
    55621 agggccatca ggaatgatct gaaactgcga atctgttaac agtctatgga gagctttcat
    55681 agaactaaga ttcggtttat ctttgctgcc acaaattagt aaggttggat aggagcaagt
    55741 ccctgctata tccgttaaat caagtgtctt caattcctca gaaactccga ccataagagt
    55801 cttgtctgct ccctgttttt caaatactct tttgggaagt agtttaaaaa tcagcaactg
    55861 aagataaaat aggatattcc ctgctaattt aagcgggcat cctgacagaa tcaaagctcg
    55921 aagatttggt aaatcgtaac tggaaagttc tagtgtcagg gcagcaccta aggacaatcc
    55981 aatcaaaaca aaaggttctg tctcttgagc taggtgctga taaactcgct ctttagcttg
    56041 ttgatagtta ctaactccag aaggaaataa ctcgatagcc tcagaaggat aatctgtcag
    56101 tagattccga acttctttcc aagactctgc tgactgccct aacccatgca aaaatatcaa
    56161 tttcatctag ttctcctcaa ggcttagttc atacaagcct ctcactgcat tacagccgta
    56221 aatagcttct gcttgggtta aatctgccaa ggtcaagact ttctcttcta cctgtcctgt
    56281 ttctagcaaa tactgacggt aaattcctgg taagattcca agtcggatag gcggtgtgta
    56341 gagttttcca gcgattttca gaaccaaatt tcctatagag gtttcaagca gttctcctga
    56401 cttattgtgg taaatcttct cttgttctcc taggctcaaa tgcggtcggt gagtggtttt
    56461 aaagtaggta aaggattgat tcaaagcagc ttcctgaagg cagacttggg cctgacaaaa
    56521 gcttgtactg agaggggtta atacttgacg attgacttct atctctccag atttgctaag
    56581 gctgattcgc aagcggtaat ctcgattagc ttcacaatcc tgacactctt cctcaatctt
    56641 gtgtcccaag tcttctgcat caaaaggaaa agcaaaataa cgactagctt ttctcagtct
    56701 ttccagatgt tgatcttcaa acagcaggtt cttctggctg atttcccctg tcgtaatcag
    56761 ttggaagcga gcttgtttac gataaagaac tgctgccttt tgatgaacct ctcggtattc
    56821 agattcccat gtgctatccc aagtaatccc tccgccaact ccataaatgg cttgaccttt
    56881 gtgaagttga atggtacgaa tggccacatt aaaaatccgt cgtccatttg gaagcaagag
    56941 accaatcgtt ccacagtaga ctccacgcgg ttgaggctcc aagtccttga taatctccat
    57001 tgtcgcaatt ttcggtgcac ccgttatgga accacaagga aagagtgagc ggaagatttc
    57061 aacaaggtcc acatcctctc gcaactgact cttgatggtc gaagtcatct gccaaacagt
    57121 tgaatactgc tctacctgac acagacgctc cacgtgctcg ctcccaactt cagaaatacg
    57181 gttcatatca ttgcgcaaga ggtccacaat catcatattt tcagagcgat ttttgggatc
    57241 ctgttccaac caactggcct gttcaagatc ttcttggtca gttaccccac gctgagtcgt
    57301 ccccttcatt ggtcgtgttg tcaactcgcg atcattttgc tcaaaaaaga gctctgggct
    57361 catggaaatc actgccatct cgtcatgttc cacataggca ttgtagcccg cctcctgctc
    57421 taccaccata cgattgtaga tggcaaaagg attggcactt aacttttgct tgagttggac
    57481 ggtgtagttg acctgatagg tgtctccctg cctcaagtga tggtgtatct gggcaatggc
    57541 cttttcatag tctgctgcag acgtcacttc ctgccaattt gagggcaaat caatatcctc
    57601 ataagtcaga ggaatagggg aagtttctac gctatcatga acagtaaagt aaagcaggta
    57661 ctctcccagt aggggatcct tgtgaactgc taatttttcc tcaaaagcag gtgcagcctc
    57721 gtagctgaca taccccacca cataataacc ttgctcttgg tagctttcca cttgtgccag
    57781 caaatctgcc acttcttcta catttctcgt tttcaactct ttaataggct gggcaaaggt
    57841 atatctctcc cctaaagtcc taaaatcaat cactgttttt ctatgcatac cttaagtata
    57901 gcataaaaag gcagatacag acaaagctga tctacctttt tacgatttta ttcattctat
    57961 gcttgtggtg cagtaaatag atcaccagag aaaccgtctg tgtttttaag gagagcttta
    58021 aagactttcg atttcaagac aactgtattt cggtatttgt ctttaatact actatcgttt
    58081 agttccgtta aatctttagt aacagcctca tcaaacatag tcttcaactg ttcataggtt
    58141 gaaatttgag tgccattaat ttcaattggt gcaaaacctt ttttagattt tgtaagaact
    58201 tccccatacc attgcttctt ccaatcttca aggtttgaaa attggttatt agaaactttc
    58261 ttaataatga aatcatcacc taatagaccg ttattttctt tattggctgc accttgatgt
    58321 ttgcttgaaa catagtcaat gaacccatgt tcatatccat agtaacccca catacggaag
    58381 gcgttatgtt tgaaggatag agaaccaggg gcaccttgac tcgtattacc gccataaata
    58441 ccagccatca tattaacatt gacatatgcc gtaccaaagt cctctgggtg gaagacctta
    58501 ttacctggat ttccgtgaat ggtcatgaaa ttattattta ccaagtcatc gatggtttca
    58561 aggttagttg ttttctcagc gtctatcaaa tcacgcactt tgtcccattg gtgaggacca
    58621 cttagtttat ttctgtccat tggtcgacgt gtttcgcgat caattttctt gaaccactta
    58681 ctattgtcac ctttaacctt aggaagaaca gcctcagctt ctgcataatc gagcatcatc
    58741 atagcttcat tgtagttctt catgtaatgg tcgatttggt cacgtgtttg aagtttggta
    58801 ggatctggat tataccattg gtttccatca tttgaacgtt caaatgccat atttaatcca
    58861 agtgcgccat attcaccttg atgtccatgt tctgctggag tttgtaacat accttgagca
    58921 aatgcttcaa gatctgtacc ctgtcgatgt ctatgacctc ccagatatgc cattcggtca
    58981 tttacgtggg tagtctcatg tgtaaaggca gatgtgccat attcacttat taaatccgac
    59041 ataacaaaat atacagcatc cataggctgt ggatcagcaa agatgtaagc atacgctcca
    59101 tacccatact tataatcatg atacttatca atacgtccaa agaattcacg aataggtgca
    59161 taatccggtt tatcttttcg atgaccaaat ctatcaggcc aaccaccggg tgcaccatga
    59221 ttatcccata caggagttgg aactacattt tgactcttaa gaagtttatc gcggacatta
    59281 tcagtggcaa ggcgtgacca aaaatctaag taattaactt gctctttagc acgtttattg
    59341 atttcactct tgaacgattc acgtttttct tctgtatctt taccatactt ttcaaacgaa
    59401 ctaaatgaga tagtattata tgttgaaatc ataaataaat gtgcatcttt caagttcaga
    59461 agcgataaaa tcatgcgtcc atgttgtccg ttattaagtc cttcatatag acgatatttt
    59521 ttattagcaa atgcagggtt agttgacggt tgttcaacta cataagcatt attttcaata
    59581 gctttcttaa accaaacatt tatgtctgtt tcatttgtga atagtttcat attgtaggtc
    59641 aagaagtcat gtaagtttcc agaaagagta tttttcgcca ccacttcacg gaaggactcc
    59701 tgtgtacgac ttcccttaat atagtcctct tttgaaccaa tttctattaa tcgatctaaa
    59761 acacttacat tcttaccata aaaatctggt ttgaatagca ttaattcttt aatattgaag
    59821 ttatcaaatt tcacaccgta ataacgattt aggtatgtta atcctaacag gacagcagct
    59881 ttgttatctt caattttctt aatcaaggca cgttttgcag cttcatctgt attcagttga
    59941 tgatcttcat tttcaactaa ttttgtcaca aatgtattaa gatgatcttt tacttcttta
    60001 aagctttctt ctaagtaaag attcttaatt gcattaactc tgttatcccc agttcttcct
    60061 aatcgttgat aaattggatc agattgtaat tcaattggac ttaaaatacc ttcaatagca
    60121 ctaattaaat ctgcacgatc tttaacaacc atatttggtg tatagacgac ttcaccaagc
    60181 tctgcgacac tgtattcttt tacttgctta acttgagagt ctttaggaga aatcgtaaag
    60241 atatcttttg tcttatctgc gtaatgaacc ataatatggt cagcatctgt aaggtctgta
    60301 ataaagtcat tacctttcat tgcagtcaca gacaatactt ctttattcat taatggacta
    60361 tcagttgtaa gcttgttccc ttgattcaca atccattctt tgttgtagaa cggttggagt
    60421 ttttcaacat tacgataaac aagctcacga tttgcattat aatcttgaat tgttttgtat
    60481 tcttcatcac gtaatatacg gcgattcaac ttatcaacca aatgctcagt tacttcaaat
    60541 gtatcagcag tgatacccat atccttaatt ttcttatcag cttctacctg tgaaatttct
    60601 ttaattttag acgattgttt atgggtttta tctccagaac tcacaccgat aactgttgca
    60661 ttattaggtt taatccaatt gcttacaaat tcatcatcca tatcattaga accaaagaaa
    60721 atatcaccac gcttaacatt cactaagctg atattgttat caatggttcc ccaaggccag
    60781 tctttaccaa ttaaaccgcc tacatcacct ctagcaacat caatagtacc tttggctaca
    60841 gaatttctta cattagacca agcaccaaca tctcttggat taaaatcatg agctaaatgt
    60901 cctactatag cacctgcacg agtatttaaa cctttaagtt caacatctgc ataagcttta
    60961 tttaaattgc ctcgataaat ctcagatgca atcccagcca agttccaccc tttatcacca
    61021 acagattgaa cttttccgat aaaagcaaca ttagtcataa tacctgcatc atctaattta
    61081 tccaaaatac ccgcaacatt gttattacca acgatagtac ccgttacttt aatattttca
    61141 acagttgcat ttttcactgt ttgagctaat gaagctaatt gatttgccca aggcatatta
    61201 atattaacgt taattaaatt aatatttttg atagaacccc cttcaactct atcaaataat
    61261 ggacgagcag tgttatgaat ggagaaatgc ttaccatcaa cacttgttaa gtgacctgta
    61321 aatttacctg ttacatatga tttacttggt gtaggtacat ttgttgcatt taaatccgat
    61381 cctaatttaa actcaccgtt aggatttttc tgcatagcat ctactaaatc tttgaagtta
    61441 tagtaaacgt tattttgcga tgctttgggt ttctctatgt aataagtgta tgtttgactc
    61501 aaactattat ttgaatcacg ttggattaaa tcaggagctg aagcagtaac tttaaataag
    61561 gatgtctcct cctcagttac ttcctctata ttagtaatgg caagccttgt tactttatta
    61621 tgattagtgg taatttttag ataatatgac gttagatttt ctggaacagt ttctagaagg
    61681 ctactatctg tttcatttcc attctcatcc actttcatca atcttgtttc tttaatatct
    61741 ttaatctcaa cttttttaag gtctaactga acttctttat cttctaatat ttcagtttct
    61801 tcaccgttac ctcgatcata gaccatggtt gtggatagtg tataaccttg ataatatttc
    61861 aagttgtcta aagtagcttg taagttattt tcttctaccg ttacttcttt aacaactgtt
    61921 ccagtattat cttttagagc tactttaatc gatttaattt ctactccagc tggtttagtc
    61981 aatgtataac tagctgtcgc actcttcttc aagacatctt tatctgtttt agcccattcc
    62041 aaggttggtt tctctaactc ttttgtgcca ttcttaataa ttttgtcttg aggtttttca
    62101 atcactgttt ctgtgatagt aggagcgtca cttgtttttt ctccgcgaat tgttttatag
    62161 gtttcagtaa tttgtttctt atcgtttaca ccttcttgag ttacttgtct tgttccgcgt
    62221 tttaaagtgt catcttcttg ttcttttgtt tcaaaaggaa tgatttcttc tcttgtctca
    62281 accgttgtcc cttcaattgg ttttgtacca cggattacct gttgatttac aggctccttg
    62341 gtgacttccg tactggttga aagaacttgg tcagttttaa cccctcctat tgttttatag
    62401 gttgtttttg taacttgact tcctttttca ccatttcgta ggacagtttc ttcgtccgtg
    62461 tacttggttg ggtcctcagt cgtttcagtt ttatagtcaa gttctgtagt tgattcttca
    62521 acgagtgttc cttcagtcac cttatactca ggtaattgtt ctggaacaag agcctcaccc
    62581 acgtaatctt cttcttgcgt ccccttagct gttttagtct cctcttttgg ttctacagtt
    62641 tcttcagcct tggtatctgg agttgactct tcttgtttcg gtgtctcttc cgctccccta
    62701 tcttctttag gattgtcttc ttgtgatgat tcttttggtg tacttggttg ctcagcttgt
    62761 gtaggttgca cgggttgttc tgggactttt gcttgttcta ctggtggttc aacttttgat
    62821 tcctcagctg gtttgtctga tggttgactt tctggcttaa ctgctacttt ttcctctgat
    62881 tttgactcaa cttctccacc tacttcttca actggagctg gttctgctga atcttctttc
    62941 ccctcttcta ctttaggaag ggtgtcgtca gtaggtttta cctccgattt tggttcttcc
    63001 tttggacttt cttctgttct aggtgcttct tcttttggag tttcctctgt ctctactact
    63061 tggttttctg tcctagcttg ctcctgattt gttgttgatt gaggagtctc aacttcgact
    63121 acagtcacct ctccaggttt tgctgaggtt tcttctaaaa cagtgtccaa gccaagcgtt
    63181 ttgaggatgt cacctgatag ataaccaaca tagcgatagc catccatttc aacaacaccc
    63241 tctcgactag ccagcgctaa ggtcgcaact gggtctacag cccctgcact aggaagaact
    63301 accaatccca tagctccaac tagaaagacg ctagcaattt tctttctctt gtagattaaa
    63361 agcaagctcc caacagtcag caaaccaaaa gctgtcaaaa cagatgcttc tgtccctgtt
    63421 tgaggcaact gatctttttg atacaccaaa ccatatacaa cttcattcct gtcaggcttt
    63481 cctgtctgaa ttaaatcttt agcttcttgt gaaataatct ctttatttac atagtgatag
    63541 gtagctgcgt ccactacaga aggagccatc aaaaggcttc caagaaatac agagcctaca
    63601 actcccttaa tcttacgaat tgaaaaacgg tcttttttaa acactttcat ctcctttatt
    63661 cattctcaag actttctaat agcatcttgc ggatagtgcg cacgcgcacc tccgattaat
    63721 tttggacgac tagccagtgc cgttacatgg gcatgaccaa tctctctcaa aatagggcga
    63781 atcggaacct gaacatgctt gacatgcatg ccaattgcag tgtctccgat atccaatcca
    63841 gcatgagcct tgataaattc aacctcaact ggatcctgca taaacttaaa ggctgccaac
    63901 tgccccgaac ctcctgcatg aagagtagga tggacactga caatttccag atcaaactgc
    63961 tctgccacct gacgttcaac aacgagagcc cgattgacat gctcacaacc ttgaactgct
    64021 aaatggatac ctctactacc tagaataccc aagatagtct ccactatcag ctcaccaatc
    64081 tcttgactgg attctttccc aatctgacca cctagcacct cactagaaga tagacctaaa
    64141 acaaaaagga ccccctgctt caaattggtc ttttctaaaa catcttccac tacctgacgt
    64201 gtttctcttt gaatctgtgt ctcgttcatc tctgttacct ctgttgtcac tcttctatca
    64261 taccgttttt tcttgttttt agcaagatag acaacctata aagtctgccc aattacgcat
    64321 aaaactccca gaattgactg ggagttagct agtttctatt ctatttatat atatttcaac
    64381 tttcgttcct ttttggggtc tagaatcaat cttcatctgg taattggctc caaaatgaag
    64441 tttgagccgt tgatcgacat tttgaagacc aactccccca cgtttgagtt gactttgact
    64501 actatcacca gcatcttgga agccaacgcc atcatcctca atacggatga ccaatcccga
    64561 atcctgtttc tggacagaaa gtttaatatg gccctgacct tccttttcct taatgccatg
    64621 gtaaagagca ttttctacaa ggggttgtag gaccagcttg ggtaagacta aattatcaaa
    64681 ggcaacattt tcattaattt cgtattccag cttatctcca tagcgttgtt tctggataaa
    64741 gagatactgg cggacatgat tgatttcgtc agagagacaa atcaagtcct tgccttgatt
    64801 gagcgccaag cggaaatagg ttgccaagga cttggtcacc tgcaccactc gctgactatc
    64861 atgaaattca gccatccaga tgatggtgtc caaagtgtta tagaggaaat gtggattaat
    64921 ctggctcgaa agggcttgaa gttggtactg acgggtcgtt tcttcctggc tacgaatagc
    64981 taccatcaac tgatcaatct gatccaacat agcattaaat tggcgagtta cttctctcag
    65041 ttcataggca ccaacttcct tggcacgaag attttgagca ccagaagcaa tttccaacat
    65101 ggtttctctc aaatccttca aaggagcaat ccagcgttta agactgaacc acactaagca
    65161 gagacaaaca agaagagatg tgacactggc cccaagcaag gtccacaaga gctgactccg
    65221 aacctggtct aacttttcca atgatgacac gccaagtacc gtccaatcag ttcctgcaat
    65281 cttctcttga ctgacgtagg atttgtgacc aggagtataa ccctgacctg tctcgatgta
    65341 gggtttcata gcctccattt tgctagacga actataaact gtgtgttgag gatggtagac
    65401 aaattcatgg ttttcattga taatgaaggc aaagccctgc tgccccaact ggagttgatt
    65461 gagataggct tccagagttt cataagaaat atccaaacga agcacaccaa gattggctcc
    65521 ctttgcatca acaagttctt gagtgacaga aatgacccac tgactatctg atttacgagc
    65581 tggagtcaaa acaggcatag ctccctgatg aatagccttt tggtaccaat cctcagccat
    65641 catatcagag gaagttttca tctgcacact gtcatctgta gaaatgacct gaccagattt
    65701 ggtcaccagc acaacagttt tcaagtcctt atctgacttc aagatggtca aaaacaaatc
    65761 tcggattccc tcgaccttgt cttgactggg attctcagca taggccagaa catccgtctg
    65821 ctgggtcaaa ccagtcgagg tggtttctag ttttttgata taagactgaa taaagtggct
    65881 agtctggctg atggtcgttt ggctgttgcc ctcaatggtg gcctcaatgg ctgaagaact
    65941 tgattgatag tagaaagttc caaccagagc taggagaatg agaaagacca gaaagatgga
    66001 aataaccatt ctaactaaaa gagaagaacg cttcatcggt cttctccctt cttaaactga
    66061 cgaggtgtca cacctgcaat ctgcttaaaa cgttgggtaa aatagttcat atcttcaaaa
    66121 ccaaccttct ctgcgatctc ataaatcttc agatctgtag ttaaaagcaa gagcttggct
    66181 tgtttaacac gttctctcac cagataatcc tgaaaaggca agcccaactc tttcttaatc
    66241 aaggaactca gataggtcgg actaaaacct aagtcactgg ctaaagactt taaactaaat
    66301 tggctatcag ccagatgaga ctggattttc tgggccatgt ttccttcaaa cctattagtc
    66361 aataaatctt gtaactgctc ttctttctct tccttgtcta gtttttgttt gattttcccc
    66421 aacatttcct caatatcctg acgagaaaag ggtttgagca ggtagtcgtc cacacctagt
    66481 ttgacagcag acaaggcata atcaaaatca tcgtaacctg ttaaaaagac caaatgaacc
    66541 tgaggatagg tttctcgtac cagactggcc aactggatgc catttagatg aggcatgttg
    66601 atatcggtta aaatgatatc tggcacctgc ttttggatca attcccaagc ctgccttcca
    66661 ttttcagcct gaccgatgat ttccatatcg taggctgcta cattgaccag tttagtcaaa
    66721 ccttgtctta ccagatattc atcttctacg attaagattg tgtaggtcat gctctgctcc
    66781 tttaccactt actagtatca gtatagcaaa attctcctct aactgcttag gaaagacctc
    66841 ttatactcaa taaaaatcaa aaagtaaact aggaagatag ccacaggttt ctcaaagtat
    66901 agctttgagg ttgtaaataa aactgacgaa gtcagctcaa agtatagctt tgaggttgta
//
LOCUS       DAVBCV010000005        60489 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBCV010000005 DAVBCV010000000
VERSION     DAVBCV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 60489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.005"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            488..1531
                     /locus_tag="VQ516_000303"
     CDS             488..1531
                     /locus_tag="VQ516_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2510811.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1698..3785
                     /gene="topA"
                     /locus_tag="VQ516_000304"
     CDS             1698..3785
                     /gene="topA"
                     /locus_tag="VQ516_000304"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2510812.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            4006..4365
                     /locus_tag="VQ516_000305"
     CDS             4006..4365
                     /locus_tag="VQ516_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2510813.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4458..5090
                     /locus_tag="VQ516_000306"
     CDS             4458..5090
                     /locus_tag="VQ516_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2510814.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            5134..5790
                     /locus_tag="VQ516_000307"
     CDS             5134..5790
                     /locus_tag="VQ516_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2510815.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5809..6903
                     /locus_tag="VQ516_000308"
     CDS             5809..6903
                     /locus_tag="VQ516_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2510816.1"
                     /translation="MNFQLAKYSLLKRFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6915..7952
                     /gene="leuB"
                     /locus_tag="VQ516_000309"
                     /pseudo
     CDS             6915..7952
                     /gene="leuB"
                     /locus_tag="VQ516_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            7949..8218
                     /locus_tag="VQ516_000310"
     CDS             7949..8218
                     /locus_tag="VQ516_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2510817.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            8222..8581
                     /gene="leuD"
                     /locus_tag="VQ516_000311"
     CDS             8222..8581
                     /gene="leuD"
                     /locus_tag="VQ516_000311"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2510818.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8743..9311
                     /locus_tag="VQ516_000312"
                     /pseudo
     CDS             8743..9311
                     /locus_tag="VQ516_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9405..9470
                     /locus_tag="VQ516_000313"
                     /pseudo
     CDS             9405..9470
                     /locus_tag="VQ516_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9633..11564
                     /locus_tag="VQ516_000314"
     CDS             9633..11564
                     /locus_tag="VQ516_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2510819.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELMSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNESDENDDADY"
     gene            11551..12903
                     /locus_tag="VQ516_000315"
     CDS             11551..12903
                     /locus_tag="VQ516_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2510820.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSDYSAFCKMMENSEENFKAIIDEEVGRK
                     "
     gene            13078..14064
                     /locus_tag="VQ516_000316"
     CDS             13078..14064
                     /locus_tag="VQ516_000316"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2510821.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKQMHDQGLIASISVGVKDYEY
                     DFVSQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            14128..14199
                     /locus_tag="VQ516_000317"
     tRNA            14128..14199
                     /locus_tag="VQ516_000317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14161..14163,aa:Arg,seq:ccg)
     gene            14382..15080
                     /locus_tag="VQ516_000318"
     CDS             14382..15080
                     /locus_tag="VQ516_000318"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2510822.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            15071..18610
                     /gene="smc"
                     /locus_tag="VQ516_000319"
     CDS             15071..18610
                     /gene="smc"
                     /locus_tag="VQ516_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2510823.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18607..19401
                     /locus_tag="VQ516_000320"
     CDS             18607..19401
                     /locus_tag="VQ516_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2510824.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGETWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            19401..20219
                     /locus_tag="VQ516_000321"
     CDS             19401..20219
                     /locus_tag="VQ516_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2510825.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAASDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            20223..21443
                     /gene="ftsY"
                     /locus_tag="VQ516_000322"
     CDS             20223..21443
                     /gene="ftsY"
                     /locus_tag="VQ516_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2510826.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAVLEESQDSEPAVGVSQEEIEELPEEEKLEHEGTIEENNSEVLEAERPQTEETVQ
                     EKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEELLIMSDVGVQVASNLTEELR
                     YEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQDNLTVMLFVGVNGVGKTTS
                     IGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVDVPVVTGPEKADPASVVFDG
                     MERAVAEGIDILMIDTAGRLQNKDNLMTELEKIGRIIKRVVPEAPHETFLALDASTGQ
                     NALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREELNIPVKLIGFGEKIDDIGEF
                     NSENFMKGLLEGLI"
     gene            complement(21489..22976)
                     /locus_tag="VQ516_000323"
     CDS             complement(21489..22976)
                     /locus_tag="VQ516_000323"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2510827.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPAIFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(23125..23865)
                     /locus_tag="VQ516_000324"
     CDS             complement(23125..23865)
                     /locus_tag="VQ516_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2510828.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDNLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(23865..26030)
                     /locus_tag="VQ516_000325"
     CDS             complement(23865..26030)
                     /locus_tag="VQ516_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2510829.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            26203..27156
                     /locus_tag="VQ516_000326"
     CDS             26203..27156
                     /locus_tag="VQ516_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2510830.1"
                     /translation="MKNKRIFKDFQASKMNLNLYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLSLVVLGQYRLESIHLNPYYLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            27233..29221
                     /gene="uvrB"
                     /locus_tag="VQ516_000327"
     CDS             27233..29221
                     /gene="uvrB"
                     /locus_tag="VQ516_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2510831.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            29231..29794
                     /locus_tag="VQ516_000328"
     CDS             29231..29794
                     /locus_tag="VQ516_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2510832.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKTNDGYYQINGNS"
     gene            29760..30248
                     /locus_tag="VQ516_000329"
     CDS             29760..30248
                     /locus_tag="VQ516_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2510833.1"
                     /translation="MTVIIKSMETPEEIEGKPLVHWQTWREAYDGLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAMDGMKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LIKAALIALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDEQEKILELGKPIKEKRMVFY
                     SK"
     gene            30321..30788
                     /locus_tag="VQ516_000330"
     CDS             30321..30788
                     /locus_tag="VQ516_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2510834.1"
                     /translation="MSRAQLTILTNICLIEDLEAQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEASDKSEFFYPRRTEDDWEKKIF"
     gene            complement(30785..31300)
                     /locus_tag="VQ516_000331"
     CDS             complement(30785..31300)
                     /locus_tag="VQ516_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2510835.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFKPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(31312..31839)
                     /locus_tag="VQ516_000332"
     CDS             complement(31312..31839)
                     /locus_tag="VQ516_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2510836.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGSLYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(32117..32680)
                     /locus_tag="VQ516_000333"
     CDS             complement(32117..32680)
                     /locus_tag="VQ516_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2510837.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(32664..33215)
                     /gene="coaC"
                     /locus_tag="VQ516_000334"
     CDS             complement(32664..33215)
                     /gene="coaC"
                     /locus_tag="VQ516_000334"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2510838.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(33227..33916)
                     /gene="coaB"
                     /locus_tag="VQ516_000335"
     CDS             complement(33227..33916)
                     /gene="coaB"
                     /locus_tag="VQ516_000335"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2510839.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            34146..35816
                     /locus_tag="VQ516_000336"
     CDS             34146..35816
                     /locus_tag="VQ516_000336"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2510840.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            36144..37319
                     /gene="mutY"
                     /locus_tag="VQ516_000337"
     CDS             36144..37319
                     /gene="mutY"
                     /locus_tag="VQ516_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2510841.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     LIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            37374..38078
                     /locus_tag="VQ516_000338"
     CDS             37374..38078
                     /locus_tag="VQ516_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2510842.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            38071..39420
                     /gene="vicK"
                     /locus_tag="VQ516_000339"
     CDS             38071..39420
                     /gene="vicK"
                     /locus_tag="VQ516_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2510843.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            39422..40231
                     /locus_tag="VQ516_000340"
     CDS             39422..40231
                     /locus_tag="VQ516_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2510844.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            40322..40564
                     /locus_tag="VQ516_000341"
     CDS             40322..40564
                     /locus_tag="VQ516_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2510845.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            40566..40820
                     /locus_tag="VQ516_000342"
     CDS             40566..40820
                     /locus_tag="VQ516_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2510846.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            40867..41640
                     /locus_tag="VQ516_000343"
     CDS             40867..41640
                     /locus_tag="VQ516_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2510847.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(41900..42886)
                     /locus_tag="VQ516_000344"
     CDS             complement(41900..42886)
                     /locus_tag="VQ516_000344"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2510848.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGLYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            43080..45548
                     /gene="gyrA"
                     /locus_tag="VQ516_000345"
     CDS             43080..45548
                     /gene="gyrA"
                     /locus_tag="VQ516_000345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2510849.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            45548..46291
                     /gene="srtA"
                     /locus_tag="VQ516_000346"
     CDS             45548..46291
                     /gene="srtA"
                     /locus_tag="VQ516_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2510850.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
     gene            46535..47332
                     /locus_tag="VQ516_000347"
     CDS             46535..47332
                     /locus_tag="VQ516_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2510851.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGILMGLPYAFLNKNEDTYVD"
     gene            47480..47602
                     /locus_tag="VQ516_000348"
     CDS             47480..47602
                     /locus_tag="VQ516_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET2510852.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            47624..48664
                     /locus_tag="VQ516_000349"
     CDS             47624..48664
                     /locus_tag="VQ516_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET2510853.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            48835..50109
                     /locus_tag="VQ516_000350"
     CDS             48835..50109
                     /locus_tag="VQ516_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2510854.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            50119..50997
                     /gene="truB"
                     /locus_tag="VQ516_000351"
     CDS             50119..50997
                     /gene="truB"
                     /locus_tag="VQ516_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2510855.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            51265..51540
                     /locus_tag="VQ516_000352"
     CDS             51265..51540
                     /locus_tag="VQ516_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2510856.1"
                     /translation="MEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSVMANLSQGL
                     SLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            51648..52286
                     /gene="udk"
                     /locus_tag="VQ516_000353"
     CDS             51648..52286
                     /gene="udk"
                     /locus_tag="VQ516_000353"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2510857.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            52414..53754
                     /locus_tag="VQ516_000354"
     CDS             52414..53754
                     /locus_tag="VQ516_000354"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2510858.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            53732..53944
                     /locus_tag="VQ516_000355"
     CDS             53732..53944
                     /locus_tag="VQ516_000355"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2510859.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            53941..54816
                     /locus_tag="VQ516_000356"
     CDS             53941..54816
                     /locus_tag="VQ516_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2510860.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            54809..55624
                     /locus_tag="VQ516_000357"
     CDS             54809..55624
                     /locus_tag="VQ516_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2510861.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHSVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            55617..56048
                     /locus_tag="VQ516_000358"
     CDS             55617..56048
                     /locus_tag="VQ516_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2510862.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            56055..57722
                     /gene="recN"
                     /locus_tag="VQ516_000359"
     CDS             56055..57722
                     /gene="recN"
                     /locus_tag="VQ516_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2510863.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            57724..58452
                     /locus_tag="VQ516_000360"
     CDS             57724..58452
                     /locus_tag="VQ516_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2510864.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            58531..60354
                     /gene="lepA"
                     /locus_tag="VQ516_000361"
     CDS             58531..60354
                     /gene="lepA"
                     /locus_tag="VQ516_000361"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2510865.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT        18524 a        10700 c        13651 g        17614 t
ORIGIN      
        1 caaggtgcac tgttatagat tcaatacact ataatttagt gtaattgaga aaggagaaat
       61 gattgtgatt gatgttggct aggttatgtt caatgattcc taccgtctca aatcttgtca
      121 gtaaggaaaa ataaattctt caaaagtaga gattacaagg cttgtttaag aaagaattca
      181 aagaccttga caaataaaaa taaaatggtt attataaaaa atggtctgaa atagatgatg
      241 atacttttcg aaaatctttt tacgtcagct cagctttgcc ttgctgtgtt ttgagcaagc
      301 tacggttagc ttccgagttt gattttcatt tactagaaat gaaactgatg agagatatca
      361 gtagacattt gagtcaggat attatggaaa atgataaaaa gagctcgtga gattggcata
      421 tcagactact aaagtattga gtttgttagg attttagcga ctagttagct gggaaaggaa
      481 gatatttgtg acaaataata aactgtattc gttgatagaa tttagaaata aaatatatga
      541 agaattagaa ctttccagaa gtgatttagc gattttacta tgtgccatgc ttatcgcctc
      601 tatcggatta aatatggatt cgactcccgt gattattgga gccatgttaa tctctccttt
      661 gatgacacct attctgggag tggggctctc tctagctata tttgatttta aattgttaag
      721 aaaatctttt aaaatattag ctattcaaat tcttgccagt ttaatagctt caacacttta
      781 tttttatctt tctcccattt cgtatgctag ttcggaaatt gttgctagaa cctctccgac
      841 tatttgggat gttctcattg cttttgtagg agggatagca ggtattattg gtgctaggaa
      901 aaaagagacc aataatattg ttcctggtgt tgctattgca accgccttga tgcctcctct
      961 ttgtacagta ggttatgcta ttgcttctgc taatctaaaa tttatcatag gctcctctta
     1021 cctattcctc atcaattgta gctttattgt cattgcgact tatataggtg ttaggttgat
     1081 gatggttaag aaacattatt ttaaagataa tgaagaagac tctaaaatgc gtaggatttt
     1141 gcttctagtt gctgttttgc tgatgattcc gagtttcatc tctgcaacga ctttagtgag
     1201 agaaacgttg aaaaaagagt cccttaagaa atttatatca gagcagtttc aggggcataa
     1261 tattttgaaa aaaacctatt ctaaaaagac tcatacccta aagctaacca tttcaggaaa
     1321 ttatttgaca gaagaagaac tcgatatgat ttcaagtaag agaggtgact atggtttaag
     1381 tgatgtttct gttcaagttt cacaattgtc tgattcagaa caacttagta aggaagaact
     1441 ggtggagtat ttcttccagt atatcaagga taaggaagca aaagaaaagg aaaaagctaa
     1501 taagttttat acagagtctg aggagcaata atttcttgag aatagctggt ttctctcgtg
     1561 agtcttctat gtatatcaaa ggaagactga ggttttaagt ataaaacttt tcttctatta
     1621 tagtagctaa gtcttgacag ggttcaaaaa atggtttaca ctttataaag tttattactt
     1681 tgaaaaggtg tgatactgtg gctacggcaa caaaaaagaa aaaatcaaca gttaaaaaaa
     1741 atctagtcat cgtggagtcg cctgctaagg ccaagacgat tgaaaaatat ctaggcagaa
     1801 actacaaggt tttagccagt gtcgggcata tccgtgattt gaagaaatcc agtatgtccg
     1861 tcgatattga aaataattat gaaccgcaat atatcaatat ccgaggaaaa ggccctctta
     1921 tcaatgactt gaaaaaagaa gctaaaaaag ctaataaagt ttttctcgcg agtgacccgg
     1981 accgtgaagg agaagcgatt tcttggcatt tggcccatat tctcaacttg gatgaaaatg
     2041 atgccaaccg tgtggtcttc aatgaaatca ccaaggatgc agtcaaaaat gcttttaaag
     2101 aacctcgtaa gatcgatatg gacttggtcg atgcccaaca agctcgtcgg atcttggatc
     2161 gcttggtagg gtattcgatt tcgcctattt tgtggaagaa ggtcaagaag ggcttgtcag
     2221 caggtcgcgt tcagtccatt gcccttaaac tcatcattga ccgtgaaaat gaaatcaatg
     2281 ccttccagcc agaagaatac tggacagttg atgctgtctt taaaaaggga accaaacaat
     2341 ttcatgcttc cttctatgga gtagatggta aaaagatgaa actgaccagc aatgacgaag
     2401 tcaaggaagt cttgtctcgt ctgactagta aagacttttc agtagatcag gtggataaga
     2461 aagagcgcaa gcgcaatgct cctttaccct ataccacttc atctatgcag atggatgctg
     2521 ccaataaaat caatttccgt actcgaaaaa ccatgatggt tgcccaacag ctctatgaag
     2581 gaattaatat tggttctggt gttcaaggtt tgattaccta tatgcgtacc gattcgactc
     2641 gtatcagtcc tgtagcgcaa aatgaggcgg caagcttcat tacggatcgt tttggtagca
     2701 agtattctaa gcacggtagc aaggtcaaaa acgcatcagg tgctcaggat gcccatgagg
     2761 ctattcgtcc gtcaagtgtc tttaatacac cagaaagcat cgctaagtat ctggacaagg
     2821 atcagctcaa gctatatacc cttatctgga atcgttttgt ggctagccag atgacagcgg
     2881 ccgtttttga taccatggct gttaaattgt ctcaaaatgg agttcaattt gctgccaatg
     2941 gtagtcaggt taagtttgat ggttatcttg ccatttataa tgattctgac aagaataaga
     3001 tgttaccgga catggttgtt ggagatgtgg tcaaacaggt caatagcaaa ccagagcaac
     3061 atttcaccca accgcctgcc cgttattctg aagcaacact gattaaaacc ttagaggaaa
     3121 atggggttgg acgtccatca acctatgcgc caaccattga aaccattcag aaacgttatt
     3181 atgttcgcct ggcagccaaa cgttttgaac cgacagagtt gggagaaatt gtcaataagc
     3241 tcatcgttga atatttccca gatatcgtaa acgtgacctt cacagctgaa atggaaggta
     3301 aactggatga tgtcgaagtt ggaaaagagc agtggcgacg ggtcattgat gctttttaca
     3361 aaccattctc taaagaagtt gccaaggctg aagaagaaat ggaaaaaatc cagattaagg
     3421 atgaaccagc tggatttgac tgtgaagtgt gtggcagtcc aatggtcatt aaacttggtc
     3481 gttttggtaa attctacgct tgtagcaatt tcccagattg ccgtcatacc caagcaatcg
     3541 tgaaagagat tggtgttgag tgtccaagct gtcatcaggg acaaattatt gagcgaaaaa
     3601 ccaagcgtaa tcgcctattc tatggttgca atcgctatcc agaatgtgaa tttacctctt
     3661 gggacaagcc tgttggtcgt gactgtccaa aatgtggcaa cttcctcatg gagaaaaaag
     3721 tccgtggtgg tggcaagcag gttgtttgta gcaaaggcga ctacgaggaa gaaaagatta
     3781 aataagagga ggggcccgaa agtaaatttc aggctccttt tggttagaac ttgacaaaat
     3841 acatcatttt atgcgaaact agaagaagat tattttatac tcaatgagaa tcaaagagca
     3901 aattagaaag ctagtcgcag gttgctcaaa gcactacttt gaggttgaag atggaagctg
     3961 acctggtttg aagagatttt cgaagagtat taactaggag aagttatgcg tattatctat
     4021 ctaattattg gttttttatc gctgaccttg gctattgttg gggttgtttt acccttgttg
     4081 cctacaacac ctttcctttt gttgtctatt gcttgtttct ccagaagttc caagcgattc
     4141 gaagattggc tttatcatac caagctctat caagcatatg tagctgattt tcgtgagacc
     4201 aagtctattg cgcgtgaacg aaagaaaaaa atcatcgtct ctatctacgt cttgatggga
     4261 atttctattt attttgcacc tcttttacca gtcaaaatcg gtctgggtgc tttgaccatc
     4321 tttattactt attatctctt caaggtcatt ccagacaaag aatagttaaa acagtagtta
     4381 tttgccttga taaaattgaa agcatattca taacaatatg atataataaa attgaagtaa
     4441 tattcaagga gaatcaaatg atttacgaat tttgtgctga aaatgtgact ttacttgaaa
     4501 aagcgatgca ggctggagct cgtcggattg aactctgtga taatctagca gttggtggga
     4561 caacacccag ctatggagtg actaaggcag cggttgaact ggcagctaac tacgatacaa
     4621 ccatcatgac catgattcgg ccacgtggtg gtgactttgt ctataatgac ctagaaattg
     4681 ctatcatgct agaagacatt cgtttgactg ctcaggctgg aagtcaaggg gttgtatttg
     4741 gagctttaac tgctgataaa aagttggata agcctaatct ggaaaagtta attgctgcat
     4801 caaaaggaat ggaaattgtc tttcacatgg cctttgatga actaagtgat gaagatcaac
     4861 cggaagctat tgactggctc agtcaagccg gtgtcactcg tatcctaact cgtgctggtg
     4921 tgtctggcga ctccttagaa aaacgttttg ttcactatca cagaattttg gagtacgcta
     4981 aaggtaaaat tgaaattcta ccaggtgggg ggattgacct tgaaaaccgt caaaccttta
     5041 tcgaccaggt gggggtaaca caattgcatg gtactaaggt tgttttttaa aaaatagaaa
     5101 ggaactgcta gctttgggta gcagttttca cttatgtttg aaatttttaa atcctatcaa
     5161 tttaatcaag aaaaggctca tgattatggt tttatagaaa atagcgaagt ctggacatat
     5221 agttgccaga ttttgcaagg tgactttgtc atgactgtgt ccatcactgc tgataatgtg
     5281 aactttcaag tctttgacca agagactggt gacctctatc ctcacgttta tatggaaagc
     5341 atgaggggaa gttttgtcgg aaatgtccgt gaggcttgtc tggagattct ttaccagatt
     5401 cggaaggctt gttttgatgt gcaagatttt atctgtcatc agactaagcg tatcatgact
     5461 caagttcagg aaaagtatgg aaaccagttg gagtatctgt gggaaaaatc gcctgataca
     5521 gctgtattgc gccatgaagg caatcaaaag tggtatgccg tcttgatgaa aatctcttgg
     5581 aataagctgg aaaagggcag agaaggacaa gtggaagcag tcaacctcaa gcatgaccaa
     5641 gtagctaatt tgctttcaca aaaggggatt tatccagcct tccatatgag caagcgctac
     5701 tggattagtg tgtcccttga tgatacttta tcagatgaag aagtactgga attgatagaa
     5761 aaaagttgga acttaacctc taaaaaatga aatattttaa taattttcat gaactttcaa
     5821 ttagctaaat attctttact gaagagattt ttagaaaata taggatttac cacactagag
     5881 gaatatggtg ccatcttcaa atacctgatt gagaatgtca agacggatcg tcagatcatc
     5941 tattcgcctc actgtcatga tgacctcgga atggcagtgg caaatagcct tgctgctgtc
     6001 aagaatggtg caggacgtgt tgaagggact atcaatggta ttagggagcg agctgaaaat
     6061 gctgctttgg aagaaattgc agtggctctc aatattcgcc aagattacta ccaagtagaa
     6121 accagtattg tcctaaatga gaccatcaat acgtcagaaa tggtttctcg cttctctggt
     6181 attccagttc ctaaaaacaa agccgtcgtt ggtggcaata ccttctccca cgaatctggt
     6241 attcaccaag atggagtcct taaaaatcct ctcacttatg agatcatcac acctgaattg
     6301 gttggtgtta agattctgct tggaaaatta tctggtcgcc atgcttttgt tgagaaactg
     6361 agagaattgg ccctagattt tacagaagag gatatcaaac cactctttgc taagttcaag
     6421 gcactggtcg ataagaagca agaaatcaca gatgcagata ttcgagcttt ggtagctgga
     6481 accatggttg aaaatccaga aggcttccac tttgatgatt tacaacttca aactcatgca
     6541 gataatgaca ttgaagcgct cgttagccta gccaatatgg atggtgagaa agtcgaattt
     6601 aatgcgacag ggcaaggttc cgttgaagca atctttaatg ctatcgataa gttctttaac
     6661 caatctgttc gtttggtgtc ctacactatc gatgcggtaa cagatggaat cgatacccag
     6721 gatcgggttt tggtcactgt tgaaaacaga gatacagaaa ccatctttaa tgcagcaggg
     6781 cttgattttg atgtgttgaa ggcttctgct attgtctata taaacgctaa tacctttgtt
     6841 caaaaagaga atgcaggtga gatgggacgc agtgtttctt accacgatat gcctagtgtg
     6901 taaaggagaa ggctatggca aagaaaatag tagctctagc aggagacgga attggcccag
     6961 aaatcatgga ggttggttta gaagttctgg aggctctagc tgaaaaaaca ggttttgact
     7021 atgagattga cagacgaccg ttcggaggtg cagatattga tgcagcatga cctcccttac
     7081 ctgatgaaac ccttaaggca agtagggaag cagatgctat cctactagta gctatcggta
     7141 gtcctcagta tgatggagca gtggttcgcc ctgaacaagg cctgatggct ctccgtaagg
     7201 aactcaatct ttacgctaat attcgtcctg taaaaatctt tgacagtctc aagcatttgt
     7261 cactactcaa actggaacga attgctggtg tagactttgt cgtggtgcgt gaattgacag
     7321 gcgggattta ctttggagat catattcttg aagagcgcaa tgcgcgtgat atcaacgact
     7381 atagctatga ggaagtggag cggattattc gcaaagcctt tgaaattgca agaaatcgca
     7441 gaaaaatcgt tactagtatc gataagcaaa atgttctagc gacctcaaaa ctctggcgga
     7501 aagtagctga ggaagtcgca caggatttcc cagatgtaac cttggaacat cagctggtag
     7561 actcagctgc tatgcttatg attaccaatc ctgctaagtt tgatgttatt gtaacggaga
     7621 atctttttgg agatatttta tctgatgaat caagcgtctt atctggtaca cttggggtta
     7681 tgccatcagc cagtcattct gaaaatggac caagtctcta tgaacctatt cacagttcag
     7741 cacctgatat tgcaggtcaa ggaattgcca atcctatttc catgatttta tcagtttcca
     7801 tgatgttgag agatagtttc ggacgttatg aggatgcaga gcgtatcaaa cgtgctgttg
     7861 agacaagtct ggcggcagga attttaacga gagatatagg aggtcaggct tcaacaaagg
     7921 aaatgacgga agctattatt gcaaggttat gaagttagac gaaaaaatta ctctagtcct
     7981 tttgatttgg aatgtcatca ttttcttgat ttatggtatt gacaaatcta aggcaaggag
     8041 aagagtttgg cgcatccctg agaaaatctt acttatttta gcctttactt ttggtggttt
     8101 tggtgcctgg ctagcaggaa tcatctttca ccacaagact cgaaaatggt actttaaaat
     8161 agtttggttt cttgggatgg tgaccacact agtagcctta tattttattt ggaggtaatg
     8221 gatggcaggg tcttcgaggg aatacgctgc ttgggctcta gcggactatg gttttaaggt
     8281 cgtgattgca ggatctttcg gtgacattca ttacaataat gaactcaata atggcatgtt
     8341 gccaatcgtt cagcctagag aggttagaga gaaactagcc cagctaaaac caaccgacca
     8401 ggtaactgtg gacttggaac aacaaaaaat catctcacca gttgaagaat tcaccttcga
     8461 gatagatagc gagtggaaac ataaactcct aaatagtttg gatgatatcg gtattacctt
     8521 gcagtatgaa gagttgattg ctgcttatga aaaacaacga ccagcctact ggcaggatta
     8581 gaaaaaatag aaaaggagat atagtaaact gaaataagat gtaaacaaat gaattggagc
     8641 ttaacatcca tttccagcaa ttttttagaa actacagtgg actattctgg attcaacaca
     8701 ttataaaatt atgacaaaac acattcacaa gaaggctacg acattttaaa aggtgagggc
     8761 ggatgtatcg tttgccctac taaagttggt tacattatca tgaccagtga caaggcagga
     8821 cttgagcgta agttcgcagc caaagaacgt aagcgtaaca aaccaggtgt tgttctctgc
     8881 ggtagcatgg atgaactttg cgctttagcg caactcaacc cagaaattga agcattctac
     8941 taaaaacatt gggatgaaga tattcttctt ggttgtatcc ttccttggaa accagaagcc
     9001 tttgaaaaac tcaaagcata cggggatggc cgtgaagaac ttatgactga tgtacgtggt
     9061 actagctgtt ttgttatcaa gtttggaaaa gcaggtgaac aattggctgc caagctttgg
     9121 gaagaaggta aaatggtcta cgcctcatct gcttcaatga caaaacgatt gaaactcgct
     9181 atgagcaagg tgtaatggtg tctatggtcg ataaggacgg caaactcatc ccagaacaag
     9241 gaggagcacg ttcaacttca ccagctccag ttgtgatccg taaagggctt gacattgata
     9301 tagtaaaatg aagtaagagc aggacaaatt aatcaggaca gtcaaatcga tttctaacaa
     9361 tgttttagaa gtagaggtgt actattctag tttcaatcca ctataaaatc atgatgcacc
     9421 tgtcagatac ttttaactca tgggactacc gtcaggttga gtattattag gatagagaag
     9481 aagtctagtg ttaaggacat tacagctcat aatactaggc ttttttatag aaattctttt
     9541 ctttttctat aggatatggt attctatgta gaaaatatat gttaataagt aatgccaata
     9601 tttaaacatc attagtaaaa ggagttagat tgatgaataa aagaaaagtt agtttagaag
     9661 atttttataa atggtatagt ctaaataaag aagagttatt aaataaggca actgttggtg
     9721 aaaagtttaa tgataaatta aaagaagagt ttctccagga atggcctttg gataggattt
     9781 taacaatgtc aatcgatgaa tatgtaatag gaaagggaca gcaaaataag tctttatgct
     9841 acgctcttga gaagggaaaa tacaaaaatc tatttcttgg aatttctggt ggctcagctt
     9901 caaaatttgg tatttattgg aataaaaaaa caaacaaata taaagatcaa gctaataatg
     9961 agatttcaga gttggatcag cgattttcaa aattaaaatc agatttgtat gaaattatca
    10021 aagaaggtat tcgttttaac tttgaaaatc ctatttttga tatgaaaaga tcaacaaatg
    10081 aatttattgg tcgttctgct atggtgacaa aattactttg tatctatact gagggagatc
    10141 ctttctttgg tgtaaatatt aatagtcaga aagaattttg gaaccacttt gtttctcaga
    10201 caaatcaagg tggaccttat ctgcaaaatc ataaaataat tgaactgatg tccaaaactt
    10261 atcctgagtt ggagccatcg aaattaggaa ctatgctttt tgagtattct aagcttttta
    10321 tggaaaataa ggaagacaat agtacaatgg attcatcaaa caattttcgt catcaattaa
    10381 ctcaatctct attaaagtct ccaaacctta tccttcgtgg tgctcctggc acaggaaaga
    10441 cttatcttgc taaagaaatt gccaaagaat taacggatgg caacgaagat caaatcggat
    10501 ttgtacaatt tcacccatca tatgattata cggattttgt agaaggttta agaccagtat
    10561 caaatgggga tggagctatt gagtttaggc tacaggacgg tatttttaaa gatttttgtc
    10621 agaaagcaaa agaaacccaa ttgattggag gacaagataa ttttgatgag gcttgggatt
    10681 cttacttaga atatataaat gttgctgaag aaaaagaata tataacaaaa acatcttact
    10741 tatctgttaa tagtagacaa aatttgtcag taaattatga tagtggtgtt ccaggatggt
    10801 cactacctag caaatatgtt tacgagttgt ataaagataa aaattataat aagcaagaat
    10861 actacaaaag tggtggaaaa actgtcctag aaacattgag aaagagattt ggtttgaaag
    10921 actatgtttc cccaacagaa attgatactg ataagaaatt cgttttcatc atcgatgaaa
    10981 tcaatcgtgg ggagatttct aagatttttg gcgaactctt tttctctatc gaccctggct
    11041 atcgtggtga aaaaggaagt gtttctaccc aatatgcaaa tctacacgaa actgatgaaa
    11101 agttctatat ccccgaaaat gtttacatca tcggaactat gaatgatatt gatcgttcag
    11161 tggatacctt tgattttgct atgcgtcgtc gttttcgttt tgttgaagtt actgccgagg
    11221 gtcaagttgg catgttggat aaagagctga atatccatgc agaagaagca aaaattcgtc
    11281 taagaaactt gaacgctgct atcgaaaatg ttcaggaatt aaacagtcat tatcatattg
    11341 gaccaagtta ttttcttaag ttgaaggatg tagattttga ctatgaatta ctctggtccg
    11401 attatattaa gcctctccta gaagactact tgcgaggttc ttatgatgag gttgaaactt
    11461 tggaaacttt gaaaaaagca tttgaactga caaataatga gcaaaaagat caggcagtag
    11521 ctgatgacaa tgaaagcgat gaaaacgatg atgcggatta ctgataatca acacaagatt
    11581 attaaagaaa aatttgttga agaatatcct aaactaagca atcttctttt agacagaacc
    11641 ttggaaagtc tatcccaaga tgaacgtatt ttcatttttc caaatgattt gactcatact
    11701 ccagatttgg ataaaaaaca aaagattttg gaaacgatca atcaggaaat caagacagga
    11761 aatgtgattg gttttcttgg atatggtcag gaaagattaa cgatttcctc acgattttct
    11821 gatgagagta atgaccattt tttgcattat ctcttaaaca aggttcttca tatcaatctc
    11881 actagtttag atgttgcttt gtctcgtgaa gagaggcttt atcaactttt gatgtatctc
    11941 ttccccaagt atctacaagc tgctattcga aaaggtcttt ataaggaata tcatcgattt
    12001 tctcataacg acagtcatgt taagggagtg attgatgtaa gaaaccatct caagaaaaat
    12061 cttcctttca cgggaaatat tgcctacaca acgagagagt tcacctatga taatcccctc
    12121 atgcagttgg tccgtcacac tattgaatac attaagaatc agaaaagcat tggtcaaggg
    12181 gtactagata atctctcaac tagtcgtgaa aacgtatctg aaatcgtgcg tgtaacgccc
    12241 tcttataaac tagctgatcg tgctaagatt attcggggaa atcaatctaa acctatacgt
    12301 catgcatact ttcacgagta cagaaactta caagaacttt gtctgatgat cctaaaccaa
    12361 gaaaagcacg gtttagggta tcaagatcaa aaaatctatg gtattctctt tgatgttgcc
    12421 tggctttggg aagagtatgt ttacaccttg ttgccaaaag gttttgtaca tcccagaaat
    12481 aaggataaga cggatggaat ttcagtattt tctgttggga aacgaaaagt atatccagat
    12541 ttttatgaca gagaacgaaa gattgttcta gatgcaaaat ataaaaaact ggaattgact
    12601 gaaaaaggaa tcaatcgtga ggacttattc cagctgattt cctattctta tattttaaaa
    12661 gctgagaagg ctggacttgt ttttccgagc aaggacaagg ttattgataa tgagatggga
    12721 aacctagcag gttatggagc tttgttgaag aaatggtcta tccaaattcc tcagaagtct
    12781 tcatcttata gtgcattttg taaaatgatg gaaaattcag attatagtgc attttgtaaa
    12841 atgatggaaa attcagaaga aaattttaaa gcgattattg atgaagaagt ggggagaaaa
    12901 taatttcccc tctttctttt atttttaaca caagtaaaac ccgaacaata tatttcaatt
    12961 cataaaataa tttgacaaaa actgtacttt ggtttataat aaattaagga aacgtcggaa
    13021 aagacgtagt gatgcgagag cataggtagg tcattacaaa agaaacgaga catcgatatg
    13081 ttaaatgaat ttccaatttt tgattacgaa gatattcaat tgattccaaa taaatgtgtc
    13141 attaaaagcc gtgcagaagc ggatacaagt gtcactttag gaaatcacac ctttaaacta
    13201 cctgttgtgc cagcgaatat gcagacgatt ttggatgaaa atgtagcaga gcaactggct
    13261 aaaggtggtt acttctacat tatgcaccgt tttgatgagg caggacgcat tccttttatt
    13321 aaacaaatgc acgatcaagg gctcattgct tccatctctg tcggtgttaa ggactatgag
    13381 tatgattttg ttagccagct caagactgat gctcctgagt atatcactat tgatattgct
    13441 catggtcatg cggatagcgt gatttctatg attcaacata tcaagaaaga attgccagat
    13501 acctttgtca ttgctggaaa tgtgggaaca ccagaagctg tgcgtgaatt ggaaaatgct
    13561 ggtgcggatg ctactaaggt cggaatcggt cccggtaagg tttgtatcac caaggttaag
    13621 actggttttg gtacaggtgg ttggcagttg gctgctctac gctggtgtgc caaggctgcg
    13681 cgtaaaccga ttatcgctga tggaggaatt cgtactcacg gtgatattgc taagtctatc
    13741 cgcttcggtg ctagcatgat catgattggt tccctctttg caggacacat tgaaagtcca
    13801 gggaaaacga ttgaagtcga tggtgaacag ttcaaagaat attatggttc agcctcacaa
    13861 tatcaaaaag gtgcttacaa aaatgtggaa ggcaaacgta tcttgcttcc tgctaaaggt
    13921 catctgcaag acactttaac tgagatggaa caagaccttc aaagtgctat ttcttatgca
    13981 ggtggacgtc aggttgctga ccttaaacac gttgattatg tgatcgtgaa aaactccatc
    14041 tggaatgggg atgcttccca ctaagacggg gtctttgacc aaaaaacttg ttattagagc
    14101 aaatttctgt tataataaaa caagtttcca cccttagtgt aatggatatc acgtaagatt
    14161 ccggttcttg agatgggggt tcgattccct cagggtggat gtaaacatcc taaaaagcct
    14221 ttaatagggc ttttttcttt atcctgaccc aaatttgccg ctaaaactga gaatctctgt
    14281 ctaaagaatt cccaaatgct ttccagtccc gttttagagt gactcagggg gctttttttg
    14341 atataataaa aaggactgtt atcagttaga aagaggttgg tatgaaagaa ttacaaactg
    14401 tactaaagaa tcattttgca atcgaatttg cagacaaaaa tttactggaa actgccttta
    14461 ctcatacgag ttatgccaat gagcaccgcc tcttaaaaat ttcacacaat gaacgcttgg
    14521 aatttttagg agacgctgtt ctacagttat tgatttcaga atatctatat aaaaaatatc
    14581 ctaaaaagcc tgaaggtgac ctatcaaaac tccgtgctat gattgtccgt gaggagagtt
    14641 tagctggttt tgcgcgtgat tgccagtttg accagtttat caagttgggt aaaggggaag
    14701 aaaaatcagg tggtcgcaat cgtgacacca ttcttggtga tgcctttgaa gcctttcttg
    14761 gtgcccttct tttggataag gatgtggcca aggtcaagga atttatctat caagtcatga
    14821 ttcctaaggt tgaagcaggc gaatttgaga tgattacaga ctataaaacc catctccaag
    14881 agttacttca ggtcaatggt gatgtggcta ttcgttatca ggtgatttct gaaacagggc
    14941 ctgctcacga taaggtcttt gatgtagaag ttcttgttga aggtaagagc atcggtcaag
    15001 gccaaggtcg ttctaagaaa ttagcagagc aggaagctgc caaaaatgcc gttgagaagg
    15061 ggctggattc atgtatttaa aggaaatcga aattcagggg ttcaagtctt ttgctgataa
    15121 gaccaaggtc gtttttgacc aaggtgtgac ggcagttgtt ggacccaatg gatctggaaa
    15181 gtccaatatt acagaaagtc tgcgttgggc tttgggggag tctagtgtca agagtctccg
    15241 tgggggcaag atgccggatg tcatctttgc tggaacagaa agtcgcaaac cgctcaatta
    15301 tgcttctgta gttgtgactc tggataatca tgacggattt atcaaggatg caggtcaaga
    15361 aatcagggtg gaacgccata tctatcgtag tggagatagc gaatacaaga ttgacggcaa
    15421 gaaagtccgt ctgcgtgata ttcatgacct tttcttggat acaggtttgg gacgagattc
    15481 cttctctatt atttcccaag ggaaggttga ggagattttt aattccaagt ctgaggaacg
    15541 acgagctatt tttgaagaag ctgctggagt tttaaaatac aagactcgca gaaaagaaac
    15601 cgagagtaaa ctgcagcaaa ctcaggataa tctggaccgc ttagaggaca ttatctacga
    15661 gttggataat caaatcaagc ctcttgagaa gcaagctgag aatgcccgta aatttctaga
    15721 cttagaaggt caacgcaagg ctatttactt ggatgtattg gttgctcaaa tcaaggaaaa
    15781 taaggcagaa ctagagtcga cagaagaaga gttggctcag gttcaagaac tcttgatgag
    15841 ttattaccaa aagcgtgaaa aattagaaga agaaaatcaa actcttaaaa agcaacgcca
    15901 agatttacag gctgaaatgg ccaaagacca aggcagtttg atggacttga ctagtctgat
    15961 tagtgattta gaaagaaaat tagccctatc gaaactggag tctgagcaag tggccttgaa
    16021 tcaacaggag gcacaagctc gtttggctgc tttggaggat aagagaaatt cactcagcaa
    16081 agaaaagtct gataaagaaa gctctttagc tctgttagag ggaaatctag tccaaaataa
    16141 tcaaaaactc aatcgtttag aagctgaatt gctggctttc tcagacgatc ctgatcagat
    16201 gattgagctc ttacgtgaac gctttgtagc tcttttacaa gaagaagcgg atgtctcaaa
    16261 ccagttgacc cgtattgaga atgagttgga aaatagtcgt cagctttctc aaaaacaagc
    16321 agatcaacta gaaaagctga aagagcaatt agctatagct aaagagaagg ctagtcagca
    16381 aaaggaagag cttgaaactg ccaaggagca ggttcagaaa ttattggctg actaccaagc
    16441 tattgccaag gagcaagagg agcagaaaac ttcctatcaa gctcaacaaa gccaactctt
    16501 tgaccgtctg gataatctca aaaacaagca ggccagagct caaagtttgg aaaatatcct
    16561 gagaaatcat agtaactttt atgcaggtgt taagagtgtt ctccaagaaa aagatcgcct
    16621 aggtgggatt attggtgcag tcagtgagca tctgaccttt gatatgcatt atcaaactgc
    16681 cctagaaatt gcgcttggag cgagtagcca acatatcatc gtagaagatg aagagtcggc
    16741 aaccaaggct attgacttcc ttaaacggaa cagagccggt cgtgcaacct ttcttccttt
    16801 gaccactatc aaggcgcgta cgatttctag tcagaaccaa gatgctatca ctgtaagccc
    16861 aggtttcctt gggatggcag atgagttggt gacttttgat actagactgg aagccatttt
    16921 caagaacttg ctagctacga cggctatctt tgatgccgta gaacatgcgc gtgcagcagc
    16981 tcgccaagtt cgttatcagg ttcgtatggt gacattggat gggacagaat tacgcacggg
    17041 tggttcctat gcgggtggtg ccaatcgcca aaataacagt attttcatca agccagaact
    17101 ggagcaatta caaaaagaaa ttgctgcaga tgaagcaagc ttgcgttcag aagaagcggc
    17161 tttgaagacc ttacaagacc agatggctac attgacagaa agattagaag tcatcaaatc
    17221 tcaaggagaa caggcacgta ttcaggagca aggcttgtcc ctcgcttatc agcaaactag
    17281 tcagcaagtt gaagaactgg aaactctttg gaaactccaa gaagaggaaa tagatcgtct
    17341 ttccgaggga gattggcaag cggataagga aaaatgccaa gatcgtcttg ctgcaatcgc
    17401 cagtgacaag caaaatctgg aagctgagat tgaagagatt aagtctaaca aaaatgccat
    17461 ccaagaacgc tatcaaaact tgcaggaaga gctagcgcaa gctcgtttgc ttaagacaga
    17521 actgcaaggg caaaaacgtt atgaagttgc tgatattgaa cgcttaggca aggaattgga
    17581 caatcttgat tttgaacaag aggaaatcca gcgccttctt caagaaaagg ttgataatct
    17641 tgagaaggtt gatacagaat tgctcagtca acaggcggaa gaagccaaaa ctcagaaaac
    17701 aaatctccaa caaggtttga ttcgcaagca gtttgagttg gatgatattg aaggtcaact
    17761 ggatgatatt gctagtcatt tggatcaggc tcgccaacag aatgaagagt ggattcgcaa
    17821 gcaaacacgt gctgaagcca agaaagaaaa ggtcagcgag cgcttgcgct atttacaaag
    17881 tcaattaaca gaccaatatc agattagcta tactgaagct ctagaaaagg cgcatgaatt
    17941 ggaaaacctc aatctggcag agcaagaggt gcaggattta gagaaagcta ttcgctcact
    18001 ggggcctgtc aatatagaag ctattgacca gtacgaagaa gttcacaatc gtctggattt
    18061 cctaaatagc caacgagatg atattttgtc agcgaaaaat ctgctccttg agaccattac
    18121 agagatgaat gatgaggtca aagatcgctt taaatcaacc tttgaggcca ttcgtgaatc
    18181 ctttaaagtg accttcaaac agatgtttgg aggaggtcag gcagacttaa tcttgactga
    18241 gggagacttg ttgacagctg gggttgagat ttctgttcaa cctccaggta agaaaatcca
    18301 gtcgcttaac ctcatgagtg gtggtgaaaa agccttatca gctcttgctt tgcttttttc
    18361 tatcattcga gttaagacta tcccatttgt catcttggat gaggtagagg ccgcgctgga
    18421 cgaagccaat gttaaacgtt ttggggatta cctcaatcga tttgacaagg acagccagtt
    18481 tatcgtcgta acccaccgta agggaaccat ggcagcggct gattctatct atggagtgac
    18541 catgcaagaa tcgggggtct caaaaattgt ttcggttaag ttaaaagatt tagaaagtat
    18601 tgaaggatga caattaaact agtagcaacg gatatggacg gaaccttcct agatgggaat
    18661 ggacgctttg atatggatcg tctcaagtct ctcttggctt cctacaagga aaaagggatt
    18721 tactttgcgg tggcttcggg tcggggattt ctgtctctgg aaaaattatt tgctggtgtt
    18781 cgtgatgaca ttattttcat cgcggaaaat ggcagtttgg tagagtatca aggtcaggac
    18841 ttgtatgaag cgactatgtc tcgtgacttt tatctggcaa cttttgaaaa gctgaaaacg
    18901 tcaccttatg tagatatcaa taaactgctc ttgacgggta agaagggttc atatgttcta
    18961 gatacggttg atgagaccta tttgaaagtg agtcagcatt ataatgaaaa tatccaaaaa
    19021 gtagcgagtt tggaagatat cacagatgac attttcaaat ttacaaccaa cttcacagaa
    19081 gaaacgctgg aagctggaga aacttgggta aacgataatg tccctggtgt caaggctatg
    19141 acaactggct ttgaatctat tgatattgtt ctggactatg tcgataaggg agtggctatt
    19201 gttgaattag ctaaaaaact tggtatcaca atggatcagg tcatggcttt tggagacaat
    19261 cttaatgact tacatatgat gcaggttgtg ggacatcctg tagctcctga aaatgcacga
    19321 cctgaaattt tagaattagc aaagactgtg attggtcacc ataaggacca gtcggttata
    19381 gcttatatgg agggcttata atggcagata taaaattaat cgcattggac ttggatggta
    19441 ccttgctgac tactgataaa aggctgacgg atcgcaccaa ggcaactttg aaagctgcga
    19501 gtgatcgtgg tatcaaggtc gtattgacaa ctggtcgtcc cttaaaagcc atggacttct
    19561 ttctccatga gttagggact gacggccatg aagatgagta taccattact tttaatggtg
    19621 gattagttca gaaaaataca ggagaaatcc ttgataaaac agtcttttca tatgatgatg
    19681 tggcacgttt gtatgaggaa acagagaaat tatcactgcc tcttgatgcc atctcagaag
    19741 gaacagttta tcaaatccaa tcggaccaag aaagtcttta tgccaaattc aatccagctt
    19801 tgacttttgt tccagttgac tttgaagacc tgtctagtca aatgacctac aacaaatgcg
    19861 tgactgcctt tgctcaagaa cccttggatg cagccattca gaagatttct ccagaattgt
    19921 ttgaccaata tgaaatcttt aaatcacgtg aaatgttgct agaatggtca ccaaagaatg
    19981 ttcataaagc aacaggtttg gcaaaactaa ttagccatct tggaattgat caaagtcaag
    20041 tgatggcctg tggtgacgag gccaatgacc tctctatgat tgaatgggca gggctcggtg
    20101 ttgccatgca aaacgctgtt cctgaagtaa aggcagccgc aaatgtagtg acgccgatga
    20161 ccaacgatga ggaagctgtc gcctgggcta tcgaagaata tgtgctaaag gagaactaag
    20221 atatgggatt atttgaccgt ctattcggaa aaaaagaaga acctaaaatc gaagaagttg
    20281 taaaagaagc tctggaaaat cttgatttgt ctgaagatgt tgatcctacc ttcacagaag
    20341 ttgaagaagt ttctcaagaa gaagctgtgc ttgaagaaag tcaggattca gagccagctg
    20401 taggggtttc tcaagaagaa atagaagaat taccagagga agagaagctt gagcacgaag
    20461 gaactataga agaaaataat tctgaagttc ttgaagcaga aaggcctcaa acagaagaaa
    20521 ctgttcagga aaaatatgac cgcagtctta agaaaactcg cacaggtttc ggtgcccgct
    20581 tgaatgcctt ctttgctaac ttccgctctg ttgacgaaga atttttcgag gaactggaag
    20641 aactgttgat tatgagtgat gttggtgtcc aagtcgcttc taacttaacg gaggaactac
    20701 gttacgaagc caagcttgaa aatgccaaga aacctgatgc acttcgtcgt gtcatcattg
    20761 agaaattggt tgagctttat gaaaaggatg gtagctacga tgaaagcatc cacttccaag
    20821 ataacttgac agttatgctc tttgttggtg tgaatggtgt tgggaaaaca acttctatcg
    20881 gaaaactagc ccaccgctac aaacgagctg gtaagaaggt catgctggtt gcagcagata
    20941 ccttccgtgc gggtgcagta gctcagctag ctgaatgggg ccgacgagta gatgttccag
    21001 tagtaactgg acctgaaaaa gctgatccag ccagcgtggt ctttgatggt atggaacgtg
    21061 ccgtggctga aggtatcgat attctcatga ttgatactgc tggtcgtctg caaaataagg
    21121 ataaccttat gactgagttg gaaaagattg gtcgtattat caaacgtgtt gtgccagaag
    21181 caccacatga aaccttcttg gcacttgatg catcaacagg tcaaaatgcc ctagtacagg
    21241 ccaaagaatt ttcgaaaatc acacctttaa cgggaattgt tttgactaag attgatggaa
    21301 ctgctcgagg aggtgtggtt ctagccattc gtgaagaact caatattcct gtaaaattga
    21361 ttggttttgg tgaaaaaatc gatgatattg gagagtttaa ctcagaaaac ttcatgaaag
    21421 gtctcttgga aggtttaatc taatcagaag caaaaatcct gcaaggcata aacttgcagg
    21481 aaattttttt attcgaaacg accatcttga cgataggcga tatctggttg ccaagtccat
    21541 ttggcaccga atttttcaag taggtcaaag ctggcttgag gtcccatgct tccagcttta
    21601 tagtcatgaa gtggggcacc attttcagcc cagagctctt caatacggtc aatcaacttc
    21661 catgatgcac caacttcatc ccagtggcta aagttagttg agttgttatt taggacatca
    21721 taaatcaatt tctcgtatgg ttctggagaa gcaccagttg cagtcgcatc tgtacggtaa
    21781 tcaagtgagt taggagccaa gttaaattct tctcctactt gcttcccatt taggctaaga
    21841 gagaagcctt ctgttggttg aatatagatg gtcaaaatat ttggagcaag tggttctcca
    21901 aagatagaat ccatttgttt aaagacgatg ttgacatgag ttcctttttc agtcagtcgt
    21961 ttacctgtac ggaaaaagaa aggaacacta cggaatcgat cgctgtctac aaagaaggca
    22021 ccagatgtaa aggtttcagt tgttgattct ggattcacat ttggctcgct acgataagag
    22081 atgtatttca tgccatcaat cttaccagag cggtattgcc cacggataaa gtgttctttg
    22141 agttcttcat cagttggatg atagaggttt ttaaagacct taatcttttc agcacgaatc
    22201 tcgtcttttg tgaagattgc tggtttgtcc atggcgagga gcgaaagaag ttgtagagtg
    22261 tggttttgga ccatgtcacg gagggcaccg gattggtcat agtagccacc acgttcttct
    22321 acacccaagc gctccgcaaa ggtaatttga acattgtcga taaaatcctt gttccaaacg
    22381 ttttcaaaaa tcaagtttgc aaagcgaact gcaaagatgc tttggatcat ttccttacca
    22441 agataatggt cgatacggaa aatttgttct tcgtcaaatg ttgctaggag ttcgtcattc
    22501 aacttgcttg cagttgcgta atctgtacca aatggttttt caacgatcaa gcgctcaaaa
    22561 cctttgccat cgacaatgtt ttcagatttg aggtgtttgg caatggttcc aaagaactga
    22621 ggtgccatag acaagaagaa gagcttattg tgttcagctt ggtatttttc attaagctca
    22681 gcttgtaatt gacgcaaagc aatataatgt tccgaatcat tgacatcatg gctttgatag
    22741 tagaagtggc tagcaaattc ttgggcttgc tcggtactat ctgccaaatc aaggatggac
    22801 tcgacaacta cagattcaaa atattcctta ctccaaggtc tacgggcagt tccaataacg
    22861 gcaaagtgct tggaaagatt gccggattga tatagtctaa aaagggaagg gtagagcttg
    22921 cgtttagcca ggtctccact cgcaccgaaa attgtaacaa taaccttaga tgacatctag
    22981 ctacctaatt tctattttta ttcctagtct tgctaggtat gaggaaacct catttcataa
    23041 acttaattct aacataattt tccgaaaaat caaaaattcc aatacgaggt agaaaaactg
    23101 caaggaaatc cttacagttt gagtttagac gtttaagacc ttatctaaga actctttcag
    23161 acgagggtgt tgtgggttat caaagatttg gtcaggtgtt ccgtcttcaa ggaactcgcc
    23221 atctgcagta aagataacgc ggttggcaac ctgacgagca aatcccatct catgggttac
    23281 gataatcatg gtcatgcctt gctcagccaa ttccttcata acgttaagta cgtctccaac
    23341 catctcaggg tcaagggcag aagttggttc atcgaagagc atgatgtctg gattcattgc
    23401 taggccacga gcgatggcca cacgttgttt ttgaccacct gataggttat ctggattggc
    23461 attagcttta tctgctagtc caaccttttc aagcaactcc attcccaatt cctcagcttc
    23521 ttccttagtc atcaacttgt gctcaatagg agcaaaggtg atgttgtcca atacagacat
    23581 atgagggaag aggttgaagt gttggaatac catgccgata ttttcacgga cgtggtcaac
    23641 attggttgtt ttttcagtta aatcatagcc gttcacagtg atgtgaccgc tagtgacttc
    23701 ttctaaaaga ttgaggctac ggaggaaagt tgacttacca gaacctgaag gaccgatgat
    23761 acaaacaaca tctccttcat agaacttagt cgtaattcct tttaggactt cattttttcc
    23821 atagtgcttg tgtaaatcat ttacatcaat ttttaatttt gccattaacg aatcctcttt
    23881 tctaagcgtt tcgctagtct agtcaaaagt gtgataatta caagatagaa gatagcaagg
    23941 attgcataca tcttgaaact ttggtagtta cgagcaatga taatcttacc agtttggaag
    24001 agttcaacca aaccgatagc agatacgata gttgtatctt taagagcgat aacgaattgg
    24061 ttgacaaagt ttggcaacat caatttagtt gcttgtggca agataatctt acgcatggtt
    24121 tttccataag agatacccaa gcttcggctg gcttccattt ggccaactgg aacggcctga
    24181 ataccaccac gaacgatttc agcgatataa gcagccgcat tgagtgagag ggcaatggtt
    24241 ccagctacaa agtcgttaat tgggctttgt tggcctgtga tagactcgat gaagtttgga
    24301 attccccaga agatgaaggc tgcaagaatc atcaatggaa taccacgaat aacgtcaacg
    24361 aaaatctcag agatgacgcg aagagatttg tatgggctaa cgctaaacat accgaagata
    24421 attccgatga caatggcaat agcaaatgag ataagagcta gagcaagagt gataccaaga
    24481 ccgctaagga gttgtttgta gttgttttga agcaagcccc agagcgttgt ttcgtcaaca
    24541 gtacttgttg aagcagttga agattcgcta gctaggtatt tgtcaagaat cttttggaat
    24601 tcaccgtttg ctttaaggtt tgcaagtccg ttgttgaaca tttcaatcag ttctggattt
    24661 gctccttttt taacggcaaa ggctgtttca ccgattggag ttccagagat tggagttttc
    24721 aatttttgac cttggctgat agaatatttg agaacaggtt catcatccat aacggcatca
    24781 atggcaccag tgtttaaact gtcatacatt gaagaaccat cagcaaaggt tttgattttg
    24841 taaccgtatt tgctttgatt ttctgttagg aaggtttgag aagcagttcc gtttttaaca
    24901 ccgactgtct ttccttttag atcttcataa gaagcaatat tgcttgattc tttgacacca
    24961 agaatggtat tagcagtgta gtatgattct gagaagtcaa aagttgcctt acgagcatct
    25021 gtgacagaca taccagcaat gataccatcg gcttgaccag cttggacagc actgatagca
    25081 gcatcaaaac cagggttggt gatttcaatt tcaaaacctt ggtctttagc gattgcctta
    25141 atcaattcca tatcaatacc agtgtattgg ttgcttgaat tttggaaaac aaaaggtgca
    25201 aaagaagaat cgctggcaat gatatattta gccttaacag gaatagcttt taatcctgcg
    25261 agagtagttg tagttggcac tgctgaagat gatgaagcag tccatttctt gataatttta
    25321 tcaagactac catctttttt catttcagcc aaggcttggt taaattcagt aaccaggtgc
    25381 tcgtatttac ttcctttttt cacaccgaaa gcaaaacttc ctacagcttc accatccatt
    25441 tcaatatgga ggtcttgacc ttggttaatg gcatattcga taacaggttt gtcatccatc
    25501 atggcatcga tggcaccagc actcaagctg ttgttcatta aatcaccagt gtcaaatgtt
    25561 ttaatagtaa agccgtattt atctttgatt gtttcaagga aacgttgagc ggcagttccg
    25621 tttttaacac caacggtttt gccagttaat tggtcgtact tgctaatttt gtgtgacttt
    25681 gtagtagcaa tgacaacttt tgtatcatag taagtatcag acatggtgaa gactttttca
    25741 cgttctttag tctttgtcat ccctgccatg atagcgtcgg cttgcccagc ttgaaccgca
    25801 ttgactgctg cgtcaaatcc aggataggac atctgaatgt tccagccttt aatctcagcg
    25861 actttgttaa taatgtcaac atcaattcct ttataagttt gatctgaatc tttaaactca
    25921 aaaggtgcat aggcggtatc agaaacaatc ttaatcgttt ctgctttggc aatacctaat
    25981 gagaaaattg ggaataaaat tagcaaaaat gctagaaatt ttttcttcat ttttagtctc
    26041 cttttccgaa tattctctca ttatatcaga aaaagtaaaa aaaggcaaat ttttatattt
    26101 ttcgtgtcag aaaatataac attgattttt ccttttcttt cttgaattgc tattaaaaat
    26161 tgctataata atactatata tagaagaaaa gaggacgggg atatgaagaa caaaagaata
    26221 tttaaagact tccaagcttc aaaaatgaat ttaaaccttt acacaagccc cttgttagcc
    26281 tttgtttttg tcttcatagg agagtttgtg gcttatactt tatatggtat tagtttgtta
    26341 gctctcatcg gacttgctag aaattttgga gaggctggtc aaaatcttgc aagctacttg
    26401 cagaccttgc atcagagctt gacggataaa acaagtgact ttcgtttaat tttaggatta
    26461 ctggcctttg gttttattct taacactgtg ttcagatgga caagaaaagt tgagaaaaga
    26521 cctattcgaa ccttgggatt ttatagagag aattgcctca gcaatcttct gaaaggattt
    26581 agtctaggcc tggcactttt tcttctgacc ttgttaagtt tagtggtctt aggtcaatat
    26641 cgtttggaat ccattcactt gaatccttat tatcttgcct ttgtcgtctt tactatccca
    26701 ttttggattt tacaggggac agcagaagaa gtggtggccc gtgcttggct acttcctcaa
    26761 ttggcctcaa gaaccaatct aaaactagct attcttatat ctagcctgtt ctttaccctg
    26821 cttcatatgg gcaattctgg cctcacccct ctatctctag taaatctctt tttattcgga
    26881 gttgccatgg ctctttacct tctcaaaact gatacagttt ggggtgttgc aggtattcat
    26941 ggtgcttgga attttgctca gggtaatctc tttgggattt tagttagtgg tcaaccgtca
    27001 ggaacgtctc tgatgacctt tttaccacaa ggcaatcaag attggctatc aggtggttct
    27061 tttggcatag aaggttccat tatgacaagt ctggtattac tactgctgat tgtctatctt
    27121 gctaataaat taaagaaaga aaatgaaagg atgtgacttc ggtccgtcct tttcttcgtg
    27181 aaaatactat aagtatgcta aaataggaat agcacatgga gagaggattc ttatgatcaa
    27241 tcacattaca gataatcaat ttaaactagt atcaaaatat caaccatcag gagatcaacc
    27301 ccaagctatc gagcagttgg tggataacat tgagggggga gaaaaagctc agattctgat
    27361 gggggcgact ggaacaggga agacctatac tatgagtcag gtcatttcta aagtcaataa
    27421 accaactctg gttattgccc acaataaaac tctggctggt cagctctatg gggagtttaa
    27481 ggaatttttc cctgaaaatg cagttgagta tttcgtatcc tactatgatt attaccagcc
    27541 agaggcctat gtcccttcta gcgataccta tattgagaag gatagttctg tcaatgacga
    27601 gattgacaag cttcgccact cagctacctc agcccttttg gagcgtaatg atgttattgt
    27661 cgtggcctca gtctcttgta tctatggttt gggttcgccc aaggaatacg ctgatagtgt
    27721 cgttagtctc cgtcctggtc tagagatttc tcgtgataaa ctcttgaatg acttggtcga
    27781 tattcagttt gaacgcaatg atattgattt ccaacgcgga agatttcgcg ttcgtgggga
    27841 tgtggtagag attttcccag cttcccgaga tgaacatgcc tttcgagtag aattttttgg
    27901 agacgaaatt gaccgtattc gtgaagttga ggctctgaca ggtcaggtgt tgggagaagt
    27961 ggatcattta gcgattttcc cagcgacaca ctttgtgacc aatgacgacc acatggaagt
    28021 tgccattgca aagattcagg ccgagttgga agaacaatta gctgtctttg aaaaggaagg
    28081 taaactgctt gaagcccagc gtttgaaaca gcggacagag tatgatatcg aaatgttgcg
    28141 tgagatgggc tataccaatg gggttgaaaa ttattctcgc cacatggatg gacggagcga
    28201 aggagagcct ccttatacgc ttctcgactt cttcccagat gatttcttga ttatgattga
    28261 cgagagtcat atgaccatag ggcaaatcaa gggcatgtac aatggagacc gttcgcgtaa
    28321 agaaatgctg gttaattatg gtttccgttt gccgtctgct ttggacaatc gtcctctccg
    28381 tcgggaggag tttgagagtc acgttcatca gattgtttac gtttcagcga cacctggtga
    28441 ctatgaaaat gaacagaccg agacagtgat tgagcaaatc attcgtccaa cgggactctt
    28501 ggatccagag gtggaagtcc gtccgactat gggacagatt gatgacctct tgggtgaaat
    28561 caatgcccgc gttgaaaaaa atgagcgtac ctttatcaca actttgacca agaaaatggc
    28621 agaggacttg accgactact tcaaggaaat gggtatcaag gttaagtaca tgcactcgga
    28681 tatcaagacc ttggaacgga cggagattat ccgtgacctg cgcttgggtg tctttgatgt
    28741 cttggtcgga attaacctgc tccgtgaagg aattgacgtt cctgaagtga gcctcgtagc
    28801 tattctcgat gctgacaagg aaggtttcct tcgcaacgaa cgtggactca tccagactat
    28861 tggacgtgct gctcgtaaca gtgaaggcca tgtcatcatg tatgcagaca cggttaccca
    28921 gtctatgcaa cgtgctatcg atgaaactgc ccgccgtcgg aaaattcaga tggcctataa
    28981 tgaagagcat ggtatcgtac cacagacaat caagaaagaa atccgtgacc tgattgctgt
    29041 gaccaaggca gttgctaagg aagaggacaa ggaagtcgac atcaatagcc tcaacaaaca
    29101 agagcgcaaa gaactcgtca agaaactaga aaaacaaatg caagaagccg tcgaagtact
    29161 tgactttgaa ctggcggctc agatccgtga tatgatgttg gaagtcaagg ccttggatta
    29221 ggggaatagt atgatttatt taagaaagtt aaagagagaa gatttgatgt ctttatggga
    29281 aatggcttat tcacaactta atccagtttg gaaacagtat gatgctccct attatgatga
    29341 ttatcagtat ttttcaaatt ttaaagaatt cgaactacaa aaatcagaat ccattttaag
    29401 caactcaaat cgccttggta tttttgttga tgataaacta gttgggactg tttcgcgtta
    29461 ttgggtatgt aaacaaacaa gatggatgga attgggaatt ggtatttatg ataaaaaatt
    29521 ctggaacact ggtattggga aagttgctat gttgcagtgg atagatagga cgtttcagga
    29581 ttacttggag ttggagcatc tgggtttgac aacttggtca gggaatcttg gtatgatgaa
    29641 acttgctgaa aaattaagaa tgaaaaaaga agctcatatt ccaaaagttc gttattatca
    29701 aggtaaatat tttgatagta ttaaatatgg tattttgaga gaagactggg agaaaacaaa
    29761 tgacggttat tatcaaatca atggaaactc ctgaagagat agaaggtaag ccccttgttc
    29821 actggcaaac gtggagagag gcttatgatg gccttttgcc tgcagaattt caggagacaa
    29881 tgacattaga aagatgtcga ttctttagtc aaaagtatcc agaaaataca ttgattgcga
    29941 tggatggtat gaaggtagtt ggttttatca gttatggcaa ctttcgtgat gagactattc
    30001 aagctggcga aattattgct ttatatgttt taaaagacta ttatggaaaa ggtatcgcac
    30061 aaaagttaat aaaagcagct ttgattgctc ttaatcattt ttctgaaatt ttcttatggg
    30121 tattgaaaga taataagcgc gcaattgctt tctatcaaaa aatgggcttt acttttgatg
    30181 aacaagaaaa gatacttgaa cttggaaagc ccataaagga aaaacggatg gtattctatt
    30241 ctaaataatt ttaaaaagta gaagctaatg gtactggtat caagtctaaa aatttaataa
    30301 attagaaagt gagtaaattt atgtctcgag cacaattaac gattttaaca aatatttgtc
    30361 tgattgaaga tctcgaagct cagcgcgtgg tgatgcagta tcgcgctcct gaaactaatc
    30421 gctggtctgg ttatgccttt ccaggaggcc atgtagaaaa tggcgaatct ttcgcagagt
    30481 ctgtcattcg tgaaatctat gaagaaacag ggttgactat ccaaaatcct caacttgtcg
    30541 gcattaaaaa ttggccacta gatacaggtg ggcgctatat tgtcgtttgt tataaggcaa
    30601 ctgagttctc tggtaccctt cgctcttcag atgaaggaga agtttcttgg gtgcaaaaag
    30661 accagattcc aaacttggat ctggcctatg atatgctacc tttgatggag atgatggaag
    30721 catcggacaa atctgaattt ttctaccctc gccgtacaga agacgattgg gaaaagaaaa
    30781 ttttctaatc ctttactaaa taacctagct gatccaaggc ctcctcgata tagtggaggt
    30841 cttgttgtgt ttcggcttcg actaggtggt aatggacacc atctgtcaat tcagataaag
    30901 gtttaaaatc agaatgctcg acttgttcta gaaaatgttg aacgtctcgg cggcaagaca
    30961 gtttaagcaa ggtttcaatt tctccataaa cgggatggtc aatcaaagta ttctggacat
    31021 gcccaccatt atcgacaata gcaaggagtt cctgaccgat ttcttcaact tcatgtttca
    31081 ctttgaaaag tttgtgaaca taaggattgg tatcaatttg cttatagatg tagccacgat
    31141 tggtggatag gataggagcg ccatcggctc tcaaaattgc aatgtcctgc acaatgacct
    31201 gacgtgtgac atgaaaatgt tccgccaaac tttggccatt aagggcttta ggagcttctt
    31261 tcaagagttg gagcagggct tgtttgcgat cttttgtcat agcttttcct tttaacggcg
    31321 ttttcgaagc actttataga cagctagtgc taatgtatag tctaccatac tatggataat
    31381 tgtgccaaat ccaactagta caaatagaac atagaacatg ttttctacat tggtacccga
    31441 agttgcgtaa aaaaggacac aggctaatac ttcagcaagg gcatgaacaa cagccaaaac
    31501 aaaattgaaa acccaggaag attttggttt atctagggta tcggggaatt tttgtaggta
    31561 aagagatcct aaagcaccaa aagatatatg ggaaaaagcc cgaaaaacta taaccatggg
    31621 atagccagcc atcaaaaatc caaaactaga ggctaggatg acaaaaactg ccatcaaggg
    31681 cgacaagaac atggctataa aaatagcgat gtggctcccc aaagtatagg aagcaggtgg
    31741 aatgacaatc ttgaaaggca taacaattgg aatcaaaatc gcaatagccg ttaaaagggc
    31801 tgtcattgtc ataaattgtg tctttttccg tgtattcaca agaatctcct ttttaactgc
    31861 atatacacta gtatggtaca ataaaccaga caataaagca agaatttact tgggtttata
    31921 gatcattttt tagttaaaag ttatagtaga tggaaactag aatagtccac ctctacttct
    31981 aaaacattgt tagcgatttg actgtcctga tcgatttgtc ctgttcttat ttcgttttac
    32041 tatagtaaag atttcattaa aaagaaactg tatagagcaa aatctccacc ttcaggtttg
    32101 aaagcggaga ttgtttttat tttttcaagg tttgtagtcg tggaacaatg gctagggtta
    32161 gaattgcaga aatgaccaat tcagcaattg aatttgttga gataacggtt gccagaagaa
    32221 gttggatatt tccattataa acatttccaa ataggaagaa gattcctcca aggacaaaga
    32281 tagtatttgt caaggaacca agggctccag ctaaaatcag accagtcttg tttttcatca
    32341 gtttatagac taagtaagga gttaaaccaa tcaaaatacg tgggacgatg gcaatgatag
    32401 ctgagtagat gtttccgttt ggtacgaagg gagagaagag gtagcttgtc ggtagaatcg
    32461 taatcgtgtt aaccgtcaag ctaagtaatc ccatcaaaaa tccaagtgta accccaactc
    32521 gtggaccata aataatgctg gcaataatga caggaatatg aacaatggtc ggtttgattg
    32581 gaaatggaaa aaggttaaag ataagtgagc tcagaaagtg tatcacgagc atggtagcaa
    32641 aaaagatagc aatgggtgca atattagagc gttttttcat cgatagtttc ctttattctt
    32701 tctaaaataa ttgtgaggtc agctaaagct cctcgtccgt ggtctccaca agctagtagg
    32761 gattccttag gagcaatcag ctgatagccg taggtttcta atgttttcag attattctga
    32821 gttactggat ggtcatacat ttttgtattc atagcaggag ctattagttt gggaatatga
    32881 cttggtaggg ctagagctgt actggttacc atgttgtccg caaatccgtg agctagtttt
    32941 gcaatagtgt tagcagttgc aggtaccacg ataaataaat ctgctttttt tccaagttcg
    33001 atatgattga cctgatcagg atagggttcc ttcatgacat ccaagtggac aggattctgt
    33061 gagagtacct gtagtgtcaa aggttggata aactctgtag cagcctgagt cattaagaca
    33121 gtgacttgat ggccttgttt ttttagagaa ctgactaaat ctgccgactt ataagaggcg
    33181 attgaacccg ttacagccaa gagaatgttt gccatagttt tcctttctaa gaatgatagg
    33241 cttgaatttt ttcaaggagg agttctgcaa tttcttcttt agtctggact gtttgaagct
    33301 gatttttctc aacaaatata gctcggtgct gatctgctga aatttgagtc aggtcattcg
    33361 cgatgattaa atctgcttga ttcttgataa gactttttcg tgcaatgtca accagatgat
    33421 cttcggtaac atcaaccagc agtttgaaac caatcagatg aatagtggga ttccattcct
    33481 tgactaggga tatgattttg ggtgtctttt taaggaacaa aacctgaacc tcatcagttg
    33541 aagaaatctt ggcctgatga ttctgcttgc ttaaaaattc ttttagattg gagctagcct
    33601 gaacttcctc aagccctgtc atataaacag gagtgtagtc agaaacagcc attgagtgga
    33661 tcaagacctg ataatcctga acacgttctt gcatttctat tagaaggtcc ttggtattgg
    33721 taatttctcg aatacttagg ttaggatgag gctctggctt cagagctcgt tttgtcgtaa
    33781 ttaaacaaac ttcatgccct gcagaaagca aagtctctgt gataattttc cccaagtgac
    33841 ctgtagaatg gttagtgata gagcggacgc tatcgatagc ttcactggta ccgcccgatg
    33901 taactaaaat tttcatagca ctattgtaca caaaaataag cggtaagtaa aatgaaagtg
    33961 ataaattttt ggtaaaaacg aagatttact atagtttcaa actataaagc catataaaat
    34021 ttaagaaagg actctaaaag ccttaaaaac agagatattt acgaacgttt agagagtttt
    34081 aggatgttaa aaatcttgtt ttgtgatata atgaattatc atataagagg ttagaggaat
    34141 tttgaatgaa aacagatatt gaaatcgcac agagtattga gttgaagcca attgttgatg
    34201 ttgtagagaa acttggtatt tcttatgacg atttggagtt gtacggaaag tacaaggcta
    34261 aactcagctt tgataaaatt cgtgcagttg agagcaatcc agtcggtaaa ttgatcttgg
    34321 ttactgccat caacccaaca cctgcaggtg aaggaaagtc gacgcttacc attggtcttg
    34381 cggatgcctt gaacaagatt ggcaagaaaa ccatgattgc tatccgcgaa ccgtctcttg
    34441 gtccagtcat ggggatcaag ggtggtgctg ctggtggtgg gtatgcacaa gttctgccaa
    34501 tggaagacat caacctccac tttactggag atatgcatgc tattacaact gccaacaatg
    34561 ccctttctgc cttgattgac aaccacttgc accaagggaa tgagctggaa attgatcaac
    34621 gtcgtatcct ctggaaacgc gttgtggact tgaacgaccg tgcgctccgc catgtgactg
    34681 ttggtcttgg tggtcctcta aacggtattc cacgtgagga tggttttgat attacagttg
    34741 cttcagaaat catggcaatt ctttgcttgg caacggacat cgaggacttg aaacgtcgtt
    34801 tggcgaatat cgttattggt tatcgctatg accgtacgcc tgtttctgta ggtgatttgc
    34861 aggttgaggg tgccttggct ttgattttga aggatgctat taagccaaac ttggttcaga
    34921 caatttacgg tacacctgcc tttgtacacg gtggtccatt tgccaatatc gctcatggct
    34981 gtaactctgt tttggcgacg acaacagccc ttcacttggc tgattacact gttactgaag
    35041 ctggttttgg tgcggacctt ggtgctgaga aattccttga tatcaagaca ccaaacttgc
    35101 caacatctcc agatgcagtt gtcattgtcg caacccttcg tgcccttaag atgaatggtg
    35161 gtgtggctaa agacgctctg actgaagaaa atgtagaggc agttcgtgca ggttttgcta
    35221 acttgaaacg ccacgttgaa aatatccgta agttcggtat tccagcagtt gtagctatta
    35281 acgaatttgt atctgataca gaagctgaaa ttgcagtctt aaaagaactc tgtgcctcaa
    35341 tcgatgtacc agttgaattg gctagtgtct gggctgatgg agcagaaggt ggagtagcac
    35401 ttgccgaaac agttgttaag acaattgctg aaaatccagc taactataaa cgtttgtacg
    35461 ataatgccct ttctgtccaa gaaaagattg aaaaaattgt cactgaaatc tatcgtggta
    35521 gcaaagtgaa ctttgagaag aaagctcaaa cacaaattgc tcaaatcgtt caaaacggtt
    35581 gggacaaatt gccaatctgt atggctaaaa ctcaatacag tttctcagac aatccaaatg
    35641 cgcttggagc accagaaaac tttgaaatta ccattcgtga attggtacca aaattaggtg
    35701 caggcttcat cgttgcctta actggtgatg ttatgaccat gccaggtctt ccaaaacgtc
    35761 cagcagctct caacatggat gttgaaagcg atggaactgt actaggcttg ttctagtgga
    35821 ttgaaattga ctttaagtga gtttggaaat aatatccaag ctcatttttt ctcagactgc
    35881 aagcagttca tcaataagat ttatactctt cgaaaatctc ttcaaactac gtcagcttca
    35941 ccttgccgta ggtatatgat actgactctg tcagttctat ccacaacctc aaaacagtgt
    36001 tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga gtataagtag
    36061 atagaaaagt tatatactat ctattgaaaa tattataaat cagatacaaa gacaggtgaa
    36121 accttctgtg ttatactaga gatatgttag atttgaaaga atacggtatc gtcatgtggc
    36181 cggaggagaa agtcatttct ttccgtgaga aacttctcgc ttggtatgat gaaaacaaaa
    36241 gagatttgcc ttggaggaga agtaaaaatc cttatcacat ctgggtatct gaaatcatgc
    36301 ttcagcagac cagggtggat acagttatcc cttactacga aagattcctg gactggtttc
    36361 caactgtcga aagtctggca actgcgcctg aggagagttt actgaaagct tgggagggct
    36421 tgggctatta ttctcgagtt cgcaatatgc aggctgcagc ccagcagatt atgactgact
    36481 ttggtggcca atttccaaat acctatgaag gaatttccag cttgaaaggg attggacctt
    36541 acacagcagg agccatttcc agtattgctt ttaacttgcc tgagccagct gtagatggta
    36601 atgtcatgcg ggtcttggcg cgtctgtttg aagtcaacca cgatattggg attccaagta
    36661 atcgaaaaat ttttcaggca atgatggaga tcttgattaa tccggatcgt ccgggtgatt
    36721 ttaatcaagc cttgatggac ttagggtctg atattgagtc tcctgtaaat cccagacctg
    36781 aagaaagccc agtcaaggac tttagtgcgg catatcagaa tggaaccatg gaccgttatc
    36841 caattaaatc tcctaagaaa aagccggttc ctatttatct taaagcatta gttgtgaaaa
    36901 attctcaagg acaattttta cttgaaaaaa atgaaagcga aaagctcttg gcaggttttt
    36961 ggcatttccc ccttatagaa gttgataact tttcgcaaga agagcagttt gacctctttc
    37021 atcaggttgc ggaagaaagt gtgaactttg gccccagtcc agaagagagt ttccagcagg
    37081 actatgacct agatgttgat tggcttgatg tttgttttga tacagtccag catgttttta
    37141 gtcatcgaaa atggcatgta cagattgtag caggtcaggt gagtgacttc catgattttt
    37201 cagataggga agttcgttgg ctttcaccag aagaattcaa gaattaccca cttgccaaac
    37261 cccaacaaaa aatctggcag gcttatgcac aagccaactt agacagtagc caagactagc
    37321 tattgtgtct tctgactatt ttttggtata atagtagaga aaaaggtgaa catatgaaaa
    37381 aaatactaat tgtagatgat gagaaaccaa tctcggatat tatcaagttt aatatgacca
    37441 aggaaggtta cgaagttgta actgctttta atggtcgtga agcgctagag caatttgaag
    37501 cagagcaacc agatattatt attctggatt tgatgcttcc agaaattgat ggtttagaag
    37561 ttgctaagac cattcgtaag acaagcagtg tgcccattct tatgctttca gccaaagata
    37621 gtgaatttga taaggttatc ggtttggaac ttggggcaga tgactatgta acgaaaccct
    37681 tctccaatcg tgagttgcag gcgcgtgtta aagctcttct gcgtcgttct caacctatgc
    37741 cagtagatgg tcaggaagca gatagtaaac ctcaacctat ccaaattggg gatttagaaa
    37801 ttgttccaga cgcctacgtg gctaaaaaat atggcgaaga actagactta acccatcgtg
    37861 aatttgagct tttgtatcat ttagcatcgc atacaggtca agtcatcacg cgcgaacact
    37921 tgcttgagac tgtctggggt tatgactatt ttggtgatgt ccgtacagtt gatgtgactg
    37981 tacgacgtct gcgtgagaag attgaagata cgcccagccg accagagtat atcttgacgc
    38041 gccgtggtgt agggtattac atgagaaata atgcttgatt tactgaaaca aaccattttt
    38101 accagagatt ttatctttat cctgattttg ttaggtttca tccttgttgt gaccctctta
    38161 ttactggaaa atagacgtga taatattcag ttgaagcaaa tcaatcaaaa ggttaaagat
    38221 ttgattgcag gagattattc caaggttctt gatatgcaag gtgggtctga aatcaccaat
    38281 attaccaata atttgaatga cttgtcggag gttattcgtc tcactcagga aaatctagaa
    38341 caagagagta agaggctaaa tagtattctg ttttatatga cagatggggt tcttgcgacc
    38401 aaccgtcggg gtcagattat catgattaac gatacagcca agaagcaact ggggttggtt
    38461 aaggaagatg ttctgaatag aagcattttg gaattgctca agatagaaga aaactatgaa
    38521 ttgcgtgatt tgattaccca aagtccagaa ttgttgctag attcccaaga tatcaatggc
    38581 gaatatttga accttcgagt tcgctttgcc ttgatacgtc gagagtctgg ctttatttca
    38641 ggtttggtgg ctgttttgca tgatacgacg gagcaggaga aggaagaacg cgaacgaaga
    38701 ctctttgttt ccaatgttag ccatgagtta cggactcctc tgactagcgt aaaatcctat
    38761 cttgaagcct tggatgaggg ggctttgtgt gaaactgtag caccagactt tatcaaggtt
    38821 tctcttgatg agaccaaccg tatgatgcgc atggtgacgg atctcctcca tctttcacgt
    38881 attgataatg ctaccagtca cctagatgtg gaactgatta acttcactgc ttttattacc
    38941 tttatcctca atcgttttga caagatgaaa ggacaggaaa aggagaaaaa atatgagttg
    39001 gtgagagatt atcccatcaa ttctatctgg atggaaattg atacagataa gatgacgcag
    39061 gttgtcgaca atattttaaa taatgctatt aagtattcgc cagatggggg taaaatcact
    39121 gtcagaatga agacaactga agaccagatg attttatcca tttctgacca cggtttgggg
    39181 attcctaagc aggatttacc acgtatcttt gaccgtttct atcgtgtgga tcgtgctaga
    39241 agtcgtgcac aaggtggtac aggtctagga ctgtctatcg ctaaagaaat tatcaaacaa
    39301 cataagggct ttatttgggc caagagtgaa tacggcaagg gttcaacctt taccattgtg
    39361 ctcccttatg ataaggatgc agtgaaagaa gaagtatggg aggatgaagt agaagactag
    39421 aatgagtgaa ataggcttta aatacagtat tttagcgtcg ggttccagtg gaaattcttt
    39481 ttatctggaa acctcaaaaa agaagctttt agtagatgca ggcttgtctg gcaagaaaat
    39541 taccagtctg ctagctgaaa ttaaccgtaa gccagaagac ctggatgcca tcttgattac
    39601 ccatgagcat tcagatcata tccatggagt aggcgttttg gctcgcaagt atggtatgga
    39661 tctttatgcc aatgaaaaga cctggcaagc tatggaaaat agtaaatatc ttggcaaggt
    39721 ggattcttcg caaaagcata tttttgaaat gggtaaaacc aaaacctttg gagatatcga
    39781 catcgagagt tttggtgtaa gccatgatgc agtcgcaccg cagttctatc gctttatgaa
    39841 ggatgataag agttttgtcc tcttgacaga tacaggttat gtcagtgacc gtatggcggg
    39901 cattgtcgaa aatgcggatg gttatcttat cgaggccaac catgatgtag agattttgcg
    39961 atcaggttct tacgcttggc gcctcaaaca acgaatccta tctgaccttg gtcacctttc
    40021 taacgaggac ggtgctgaag ctatgattcg gacgctagga aatcgtacta agaagattta
    40081 ccttgggcat ttatctaagg aaaacaatat caaggaactg gctcatatga ccatggtcaa
    40141 tcagctggct caagctgatc tgggagtcgg agtagacttt aaggtttatg atacctcacc
    40201 agataccgca acaccattga cagagatata gaaagaacgc tgagaaggtg ttctttttat
    40261 attgactgga cacctaaaaa gtaatacaat ggtgttacca ttaaaaaagg gagcacaaaa
    40321 gatgactact attacattaa aagtttctga agctgataaa acatttatga aagcaatggc
    40381 taagtttgaa ggagtttccc tttcggaact tattcgcacc aaaactcttg aagccctaga
    40441 agatgaatac gatgctcgtg tggcagattt agcctatcaa gagtatttag aagacttgga
    40501 aaaaggagtt gaacccatta cttgggaaga aatgatgcat gatttaggct tgaaggatga
    40561 ataatttgta taaattagtt ccaacaagac gttttatcaa gcaattgaaa aaattggacc
    40621 gttatacgca gaagctaatt acaaactatt tacaaaccaa tgttttggaa gatccaagac
    40681 gacacggaaa ggctttggtt ggtaatcgcg ttggtcaatg gcgctataga attggtaatt
    40741 atcgagttat cgtacaaatt gtagatgatg aattagttgt tgctactcta gaagttggtc
    40801 atcggagaga tatttattaa attaaagctg agtaataaca gtatagaaag taaggagagt
    40861 aaaaatttga agaaaaaatg gttttttgca gattattatg acacaacaat tattttactt
    40921 gctttaatct ctgttattct ggtattactt ggatttgctg agatgattga tttggacaat
    40981 ccgccttata gtattattga tttagtaatc tggggtgttt ttgtaattga ttatagttgg
    41041 cgctttttta cgactaaaag aaaatggcgc tttattcttg aaaatatttt tgatttattg
    41101 gctattctcc ctttaaatgc tatttttaca gtgtttcgat tagggcgtat ctttcgttta
    41161 gctaaactaa caaaattact gaaactgact cgtttactta gaataattgg acttacaggt
    41221 aaattagaaa gaaaaattag tagattctta cggacaaatg gcctgattta tatcttgtat
    41281 gttaatatct ttattgttct agtagggagt tcgattttat ccgttgttga agaaaaatcc
    41341 ttctcagata gtctttggtg ggctcttgtg acagtaacta ctgttggata tggggatatt
    41401 gttcctgctt ccatttttgg gaaatggcta gcagttttac tgatgcttgt cggtattgga
    41461 acaataggga tgttaacaag tgctttgacg aacttttttg taaaggataa tccagatgaa
    41521 cagataaaac ttgataaact caaagatgaa ttatcgagcc aaagaatatt actagagaaa
    41581 caatctaaaa agatagaaga actacataaa atgatacaag atttgattga aaaaacataa
    41641 taaaaataaa gatgagacaa agtttgagct gaggagaaat caagtaagtt ttgacacaat
    41701 agaacgcata ttatcaggta taaaaacttg atactatgcg ttttatagag aaataaaata
    41761 aaatctgaac aaatcaatta ggaatttaaa gcattttata acaacgtttt agagtgatgg
    41821 ggtgctattt caacttcaac ctactataat acagaaaaaa acaactccct gatgattcaa
    41881 ggagttgtct atagttaaat tagtttttag aagcttcttg gaattctgga tttttccatg
    41941 cttcgtcaat aatagcttgc aattctttag cagatgcttg cattttttga gtttctgcat
    42001 cgttcaatgg aatgtttact ggacgaacga taccatgtgc accaacaaca gctggttgac
    42061 cgataaagac attctcaact ccgtatagac cttcttggaa tactgaaagt ggaagtactg
    42121 cgttttcatc gtcaaggatt gctttagtga tacgagcaag ggctactgcg ataccgtagt
    42181 atgttgcacc ttttttgttg atgattgtgt aggctgcatc acgaacacct tcgaacaatt
    42241 caatcaattc agcttcttga acattttgag tgtctttaag gaattcttca aggtttacac
    42301 cagcgatgtt agcgtgtgac caaacagcga actcagagtc accgtgttca cccatgatgt
    42361 aggcgtgcac tgaacgagca tccacatcca atttttcagc aagtgcttga cggaaacgag
    42421 ctgagtcaag tgaagtacct gaaccgataa cgcgttcttt agggaaacca gagaatttcc
    42481 aagttgagta agtcaaaacg tcaactgggt tagcagcaac aaggaagata cctttgaaac
    42541 cagattcaac aacttgagtt acgattgatt tgttgatagc aaggttttta cctacaaggt
    42601 caagacgagt ttcacctggt ttttgaggtg cacctgcagt aatcacaaca aggtcagcgt
    42661 ctgcacagtc agagtattga gctgcataga tttttttagg tgaagtgaag gcaagggcgt
    42721 gactaaggtc aagcgcatca ccaacagctt tttcatgcaa ttgtggaatt tcgataattc
    42781 caagctcttg tgcaattcct tggttaacaa gtgcaaaagc gtaagatgaa cctacagcac
    42841 catcaccgac aaggataact tttttgtgtt gtttagttga agtcattgtt ttaaacatct
    42901 ccttaatttt attaggggat tttccctaga caacttcatt ctatcacttt taaaaaactt
    42961 tgtcacgaat atgccttata gttctcgatg taaacgtttt agtggtttag aggctgaaat
    43021 agatgggaat ttatggtata atgttgttac ttactaattg tgaaatgagg catttattaa
    43081 tgcaggataa aaatttagtg aatgtcaatc tgacaaagga gatgaaggca agttttatcg
    43141 actacgccat gagtgttatc gtagcgcgag ctcttcctga tgttcgagat ggcttaaaac
    43201 ctgttcaccg tcgcattctc tacggaatga atgaattggg tgtgacccca gacaaacccc
    43261 ataaaaaatc tgctcgtatt acaggggatg tcatgggtaa atatcaccca cacggggatt
    43321 cctctattta tgaagccatg gtccgtatgg ctcaatggtg gagctaccgt tacatgcttg
    43381 tagatggtca tgggaatttt ggttccatgg atggagatag tgctgccgct caacgttata
    43441 ccgaggcacg tatgagcaag attgctctgg aaatgcttcg tgatatcaac aaaaatacag
    43501 ttgatttcgt tgataactat gatgccaatg aacgggaacc cttggtcttg ccagcgcgtt
    43561 ttccaaacct tttggttaat ggagcaactg gtatcgcggt tgggatggca accaatattc
    43621 cacctcataa tctgggtgaa accattgatg cagtgaagtt ggtcatggat aatcctgaag
    43681 tgactaccaa ggacttgatg gaagtcttgc ctggaccaga ttttccaact ggtgctcttg
    43741 tcatggggaa atcaggtatc cataaggctt atgaaacagg taaaggttcg attgccctac
    43801 gttctcgtac agagattgaa acgactaaga ctggtcgtga gcgcatcgtt gtaacagaat
    43861 ttccttacat ggtcaataaa accaaggtgc atgagcatat tgttcgcttg gttcaggaaa
    43921 aacgcattga gggtatcaca gcagtacgtg atgagtcaaa ccgtgaaggt gttcgatttg
    43981 ttattgaagt caagcgcgac gcctcagcca atgttattct caataacctc ttcaaaatga
    44041 cccaaatgca aaccaatttt ggtttcaata tgctcgctat ccaaaatggt ataccgaaaa
    44101 ttttgtctct tcgtcagatt ttggatgctt atatcgagca ccaaaaagaa gtggttgttc
    44161 gtcgtacacg ttttgataag gaaaaagcgg aagcgcgcgc tcatatctta gaaggtctct
    44221 tgattgcgct agaccatatc gacgaagtga ttcgtatcat ccgtgctagt gaaacggatg
    44281 cggaagctca agctgagttg atgagcaagt ttaagctttc tgaacgtcaa agtcaagcta
    44341 tccttgatat gcgtcttcgt cgtttgacag gtttggaacg cgataagatt caatctgagt
    44401 atgatgacct cttggctctg attgcggatt tagcagatat tcttgctaag cctgaacgtg
    44461 tttctcaaat tatcaaagac gaattggatg aagttaaacg taaattttct gataagcgcc
    44521 gtacagagtt gatgattgga caggtcttga gtctcgagga tgaggacttg attgaagaat
    44581 cggatgtctt gattaccctt tctaacagag gctacattaa gcgtttggat caggacgagt
    44641 tcactgctca aaaacgtggg ggtcgtggtg tccaaggaac gggagtgaaa gatgatgact
    44701 ttgttcgtga gttagtgtca actagcaccc atgatcatct gctcttcttc acaaacaagg
    44761 gacgtgtcta tcgtcttaaa ggttatgaaa ttcctgagta tggtcggact gccaaagggc
    44821 taccagtagt caatctcttg aaattggatg aagacgaaag tattcagacg gttatcaatg
    44881 ttgagtctga tcgcagtgat gatgcttatc tcttctttac aacccgtcac ggtattgtga
    44941 agagaaccag tgttaaggag tttgccaata ttcgtcaaaa tggtctcaaa gcgctgaatt
    45001 taaaggatga agatgagtta atcaatgtct tgttggcaga aggagatatg gatattatca
    45061 ttggtaccaa gtttggttat gcagttcgct ttaatcaatc agccgttcgt ggtatgagtc
    45121 gtatcgccac tggtgtgaaa ggtgttaacc ttcgtgaagg agacacagtt gttggtgcca
    45181 gcttgattac tgatcaagat gaggttctta ttatcacaga aaaaggatat ggtaagcgta
    45241 cagtcgctac tgaataccca acaaaaggtc gtggtggtaa gggaatgcag acagctaaaa
    45301 ttaccgaaaa aaatggcttg ctggccggtc ttatgactgt tcaaggggat gaggatttga
    45361 tgattatcac tgatacaggt gtcatgattc gaaccaatct tgccaatatt tcacaaacag
    45421 gacgtgcaac tatgggagtt aaagtaatgc gcctggatca agatgctcag atagtgactt
    45481 tcacaacggt tgcggtggca gaaaaagaag aagttgggac agaaaacgaa acagaaggtg
    45541 aagaataatg tctcaaaaaa ataataaaaa gaaaaacaag cgaaaaaatc tgctgacaaa
    45601 tatcctagca ggatttctga tattactgtc actggctttg atttttaata ctcaaattcg
    45661 aaatattttc atagtctgga ataccaataa gtatcaagtt agccaggtat caaaagaaaa
    45721 attagaagaa aatcaggata cagaaggcaa ttttgacttt gattctgtca aagctatctc
    45781 ttcggaagct gttctaactt ctcaatggga tgctcaaaaa ttaccagtta ttgggggaat
    45841 tgcaattcct gaattggaaa tgaatttgcc gatttttaaa ggacttgata atgttaatct
    45901 cttctacgga gctggtacaa tgaaacgcga gcaagtaatg ggagaaggaa attatagtct
    45961 agctagtcac catatctttg gtgttgataa tgctaataaa atgttatttt ctcctttaga
    46021 taatgctaaa aatggcatga agatttatct aaccgataaa aataaagttt atacttatga
    46081 aatacgtgaa gtcaaacgtg tgacaccgga tcgtgttgat gaagttgatg atagagatgg
    46141 ggtcaatgaa atcacattag taacctgtga agaccttgct gctacagaac gtattattgt
    46201 caaaggtgat ttgaaagaaa caaaagatta ttcacaaaca tctgatgaaa ttctaacagc
    46261 tttcaatcaa ccatataaac aattttatta atacaaatca gtgaaatcat ggatttcact
    46321 gattttttaa gattttcata aaaatatagt aaaatgaaat aagaatagga cgaatcgttc
    46381 aggacagtca aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttctagtttc
    46441 aatctactat aaacttttat gaaatacaga aaacgcttcc taaaacctaa agtttgtgat
    46501 ataattatca agaaaaaaga tttaggagtt tattatggtt tcttcagaat ttatctcaaa
    46561 gattgaattt gcttgcaata agaaagaaag tctttatagt caaagcaaat ttaagtatgc
    46621 gattcgttcg atgttcgcag gtgcattttt aaccttcagt actgctgcag gtgcagttgg
    46681 ggctgacttg attaataaaa ttgcaccagg tagtggacgc ttcctctttc cattcgtttt
    46741 tgcttggggc ttggcctaca ttgttttttt gaatgccgag ttggtcactt caaacatgat
    46801 gttcttgact gctggtagtt tcttaaaaaa aatctcttgg agaaaaacag ctgagatttt
    46861 actatactgt accttgttca accttatcgg agccttgata gcagggtggg gctttgctca
    46921 ttcggcagcc tatgcgaatc tgacacacga tagtttcatc tcaggtgttg ttgagatgaa
    46981 gttaggccgc tcaaatgaat tggtcttgct tgaggcgatt ttggcaaata ttttcgtaaa
    47041 tattgcgatt ctgtcattta ttttggtcaa agatggtggt gccaaacttt ggcttgtgtt
    47101 gtcagctatt tacatgtttg tattcttaac aaacgagcac attgcggcga actttgcttc
    47161 tttcgcgatt gtgaaattca gtgttgctgc ggattcaatt gccaacttcg gtgttggaaa
    47221 tatgcttcgc cactggggtg tgactttcat cggaaacttt atcggaggag gcatcttgat
    47281 gggtcttcca tatgccttcc tcaataaaaa cgaagatact tatgtagatt aagaaaatga
    47341 gcacgattga gtcgtgcttt tttcattttc aaaataaggt aatagctatt tcttatatca
    47401 aaatatagaa aactgatatt tgtaaactat aactcaaggt gctacaatat ccttaataaa
    47461 atgatatgga ggtcacctta tgacttgtga ttttaaattt gaaactctac aactacatgc
    47521 tggtcaagtt gtggctccag ctactaagtc tcgtgcagtg ccgatttatc aaacaacatc
    47581 ttttattttt gatgacacgt aggaaggtgc tgatctgttt gccttgagga aaccagggaa
    47641 catttatact cgtatcacca atcctacaac agctgccctt gaaggtggtg ttgaagcgct
    47701 agcaacagca tcaggtatga ctgcagtgac ttatacgatt ttggcgcttg cccatgctgg
    47761 tgaccatgta gtggctgctt cgactattta cggtggaacc ttcaatcttt tgaaagaacc
    47821 ccttcctcgt tatggtatca caacaacctt tgtcgatgtt gataatttgg aggaagtaga
    47881 agcagctatc aaagacaata ccaagcttgt cttgattgaa accttgggta actccttgat
    47941 taatattcca gacctggaaa aactggcaga gattgctcat aaacatcaaa tcccacttgt
    48001 gtcagacaat acttttgcaa taccttattt gattaacgtc ttctctcatg gcgttgacat
    48061 tgccattcac tctgcgacta agtttatcgg tgggcatggt acaactattg gaggaataat
    48121 tgtcgatagt ggtcgttttg actggacggc ttcagggaaa ttccctcaat ttgttgacga
    48181 gggtccaagc tgccacaatt tgagttatac ttgtgatgtg ggtgcagcag cctttattat
    48241 agctgttcga gttcaattgc ttcgtgatac aggtgcagcc ttgtcaccat tcaatgcttt
    48301 cctcttgcta caaagacttg aaacctcttc acttcgtgtg gaacgccatg tacaaaatgc
    48361 tgagacaatt gttgattttc ttgtcaacca tcctaaggta gaaaaggtaa attatccaaa
    48421 acttgcagat agtccttatc atgccttggc tgagaaatac ttgccaaaag atgtcggttc
    48481 aatctttacc ttccatgtca aaggtggcga ggaagaagca cgcaaggtca ttgataattt
    48541 agaaatcttt tctgaccttg caaacgcggc agatgctaaa tcgcttgttg tccatccagc
    48601 aacaaccact cacggtcaat tgtcagaaaa agacctagaa gcagcaggtg tcacaccaaa
    48661 ctaaattcgt ttgtcaatcg gtcttgaaaa tgtagaagat ttgattgaag acttgcgctt
    48721 ggccttggaa aaaatttaaa gtaaaagaag ataaacagtg ggcttcgact cactgttttt
    48781 gattttccct caggcatgat ataatggtta aagaagtcta gaaagaggaa cgatatgaac
    48841 gaaatcaaat gtcccaactg tggggaagtc tttacagtaa atgagagtca gtatgccgaa
    48901 ctcttgtccc aagtgagaac ggcagagttt gataaggaac tacacgatag gatgaagcag
    48961 gaactggcct tggctgagca aaaggccatg aatgagcaac agactaaact ggctcagaag
    49021 gatcaagaaa ttgcgcaatt acagagtcag atccaaaact ttgatacaga aaaagaattg
    49081 gccaagaaag aggttgaaca gacaagccat gaggctctct tggctaagga caaggaagta
    49141 cagctcttag aaaatcagtt ggctaccttg cgtttggagc atgaaaatca actgcaaaag
    49201 accctttctg acctagaaaa agaacgggat caggttaaaa accaactact tttgcaggaa
    49261 aaggaaaatg aattatcttt ggcttctgtt aagcaaaact acgaagccca gctcaaggca
    49321 gctagtgaac aagtcgagtt ttataaggat tttaaggctc aacaatctac aaaagcgatt
    49381 ggggaaagcc tagaacagta tgcagagagt gagtttaaca aggttcgtag tttcgccttt
    49441 ccaaatgctt actttgagaa ggataacaag gtctcttcgc gtgggtctaa aggggacttt
    49501 atcttccgtg agtgtgatga aaatggagtt gaaatcattt ctatcatgtt tgagatgaaa
    49561 aacgaagcag acggaacaga gaagaagcac aagaatgcag atttttacaa ggaattggac
    49621 aaggaccgtc gggagaagaa ctgtgagtat gccgttttgg tgaccatgct tgaggccgat
    49681 aatgactact ttaacacagg gattgttgac gtcagccacg agtatgaaaa aatgtatgtt
    49741 gttcgtcctc aattctttat ccagttgatt ggtctcttac gtaatgcggc gctaaattcc
    49801 ctaaaataca agcaggagtt ggccttggtt cgcgagcaaa atattgacat tacgcatttt
    49861 gaggaagact tggatgcctt taagctagct tttgctaaga actataattc agcttcgact
    49921 aactttggaa aagctattga tgaaatcgac aaggccatca aacgcatgga agaggttaag
    49981 aaattcctga ctacatctga aaaccaactc cgtctagcta acaacaaatt ggaagatgtc
    50041 tctgttaaaa aattgacccg gaaaaatcca acaatgaaag cgaagttcga agcactgaag
    50101 ggggagtaga aagcaaaaat gaacggtatt atcaacttaa aaaaagaagc aggaatgacc
    50161 tcgcatgatg cggtttttaa actgcgtaag attttgggaa ccaagaaaat tggtcatggt
    50221 ggaaccttgg atccggatgt ggtgggtgtt ttgccgattg cggttggcaa ggcgacacgc
    50281 atggtcgagt ttatgcagga cgagggtaag atctatgagg gggaaatcat tctgggctat
    50341 tccacgacga ctgaggatgc tagtggggaa gtggtcgcag aaacccctgt tttgtctccc
    50401 ttggatgaaa agcttgttga tgaagcgatt gctagcttga ctgggcctat tactcagatt
    50461 ccccctatgt attcggcagt taaggttaat ggtcgcaagc tctatgagta tgcgcgtgct
    50521 ggtcaggaag tggagcgtcc agaacgtcag gtgaccattt atcaatttga gcgaacaagt
    50581 ccgatttctt atgatggcca acttgcccga ttcacttttc gtgtaaaatg cagtaaaggg
    50641 acttacatcc gtactttgtc agttgatttg ggtgaaaagc ttggttatgc ggctcatatg
    50701 tcccatttga ctcgtactag tgctgctggc ttacaattag aagacgctct taccttggag
    50761 gaaattgctg aaaaagtaga ggctgggcaa ttagattttc tccatccttt agagattggg
    50821 acaggtgacc ttgtcaaagt tttcctaact ccagaagagg ctacagaagt tcgctttggt
    50881 cgttttattg agctagacca aacggacaaa gaactggctg cctttgaaga tgataaattg
    50941 ttagccattc tagaaaaacg gggcaatctc tataagccaa ggaaggtttt tagctagatc
    51001 gtttaggaat aaaaatcggg tgatagataa caattgcttg ataaaccccc atactaatag
    51061 tagaatggtt ttgggaatta taatattcca attgttgcga gttgtagata ctcaaataat
    51121 ctatatagaa atttagaggt gtgaaatgaa gcaacttaaa attctttcag ataaatattt
    51181 agagtccatg acaggttctg gtgggaactt aggtctagga tttggtgtga taattccatg
    51241 atgtgaaatg agtttcgaga aagggtggag caacttcttc aacaaaaaga aataaatgaa
    51301 aatagtgagt tgagtcacct gtttcgtctt gctatacaaa atttagacag aaatgaaaaa
    51361 taccaatcgg tcatggccaa tttgagtcaa gggttgtcac tttacctcat gacgcatcat
    51421 taccaggcac ctaagtctgt cattgatttt ggtttatgga ttgccaaagc tcctagccag
    51481 gaaagaggga gactggcttt cttgcagatg cttgctcaaa ccctacaggg ctttcgttaa
    51541 aactgtcgaa catcagaggg aagactctgg tgttttttgc tgatgagact ataaattttg
    51601 taaaaaatgt gatagaatag acgacggata aaataacgga ggatagcatg caaaatagac
    51661 caatcattat tggagtgaca ggtggttctg gtggtggtaa aaccagtgtt tcaagagcca
    51721 ttttatcgca ttttcctgat gaaaagattt ccatgattga gcatgattca tactacaagg
    51781 atcagtctca tttgaccttt gaagagcgtg tcaaaaccaa ctatgaccat ccttttgcct
    51841 ttgatacaga cttgatgatc gagcagatta aggaattgtt ggcagggcgt ccggtggaca
    51901 tcccgaccta cgactataca gagcatacac ggagtagcaa gacctatcgt caggagcctc
    51961 aagatgtctt tatcgttgag ggcattttgg tcttggagga caagcgtctg cgcgatttga
    52021 tggatatcaa gatttttgtg gatacggatg acgatgtgcg tattattcgt cgtatcaagc
    52081 gtgatatgga ggaacgtggc cgtagccttg atagcgttat taaccagtac ttaggtgtgg
    52141 tcaaaccaat gtaccaccag tttatcgagt caactaagcg ttatgctgat atcgtcattc
    52201 ctgaaggggt tagcaatacc gtggctatcg acctgttgac gaccaagatt gcaaagattt
    52261 tggaagaagc tcgaaacagc aaataatcac atgtggaggc cttgcctcct ttttctattt
    52321 ttccctcata atggtacata aatggagatt tttaggcata tttttggtat aataataccc
    52381 ataaaagagc aagtaaatga atagtaggtg gagatggaaa agtatttatc ggtaacaact
    52441 ttgaccaaat atctgaaaat gaaattcgat aaagacccat acttggaacg ggtctattta
    52501 actggtcaag tttccaactt tcgtaaacga cctactcacc aatatttctc cctaaaggat
    52561 gaccatgcag ttattcaagc gaccatctgg tctgggattt atcagaaatt agggtttgac
    52621 ctggaagaag gaatgaagat caatgtgatt gggcgtgtac aggtctatga accaagtggt
    52681 agctactcca tcatcattga aaaagctgag cctgatgggg ttggggcgct tgcgattcag
    52741 tttgaacaac ttaagaaaaa attgacagaa gaaggcctgt ttcaagaacg cttcaagcaa
    52801 gctctacccc aattttctaa gagaattggg gtagtaacca gccgtagtgg agccgttatt
    52861 cgagatatta tcacgaccgt cagcaggcga ttcccaggtg ttgacatcct tctttatcct
    52921 accaaggttc aaggtgaagg ggctgcggag gaaattgcta gaaatattgc gcgtgctaat
    52981 caacgggacg atttggattt gctcattatt ggtcgtggag gtggttctat tgaggatctc
    53041 tgggccttta acgaagaaat tgtggtacga gctatttttg aatctcgttt gccagttatc
    53101 tctagtgtgg ggcatgagac ggatgtgacc ttggcagatt ttgtggcaga tcgacgcgct
    53161 gcaacgccaa cagcggcggc tgaactggca acacctgtga ccaagttgga tgtattagct
    53221 catttgcaaa atcaggaaaa acggatggca acggcagtcc gaaatgttct atctaagaaa
    53281 caagaggctt tgaaaaaatg cagtcagtct gttatcttta gacaacctga gcgcttgtat
    53341 gacggttatt tgcaacgctt ggaccaactg caactgcgtt tgaaacaaag tttgcgaact
    53401 cggatttctg ataacaaaca attagttcaa gcaagaactc atcaattagt acaattatca
    53461 cctgttacca aaatccaacg ctatcaagac cgtttaggac agttggacaa gctcttaggt
    53521 agccaaatgg cgttagttta tgacgccaag gttgctgagg ccaagcgact ttcggaagct
    53581 ttgctcatgt tggatactag ccgaatcgtg gcgcgtggtt atgctattgt caaaaaagaa
    53641 gagtccgttg tagattcggt tgagagtttg aagaaaaaag accaagtaac gcttttgatg
    53701 cgagatggtc aagtagaatt agaggttaaa gatgtcaaaa caaaagaaat ttgaggaaaa
    53761 tctagcagaa ctggaaacca ttgtccaaag tttggaaaat ggtgaaattg ctctggaaga
    53821 tgcgattact gcctttcaaa agggcatggt cttgtcaaaa gagctccaag ctacgctgga
    53881 caaggctgaa aagaccttgg tcaaggtcat gcaagaagac ggaacagaaa gtgattttga
    53941 atgaaaaagc aagaaaaatt agctcttgtc gagtcggctt tggaagattt ttatggagac
    54001 cagcagtttg cctctagttt acgggagtct gttctctatt ctattcatgc tggtggcaag
    54061 cgtattcggc cttttctctt gttagaagtt ctggaatcct tgcaggttac catcaaacct
    54121 gctcacgcgc aggtagctac tgccttggag atgattcata cagggagctt gattcacgat
    54181 gaccttcctg ctatggatga tgacgattat cgaagagggc ggttaaccaa tcacaagaaa
    54241 ttcggtgaag ctatggccat tttggctgga gatgccttat tcttagaccc atatgccttg
    54301 attgcgcagg cagatttgcc aagtcagatc aaggtggact tgattgccaa cttatccctt
    54361 gcttcaggta gtctgggtat ggtggcaggg caagttttgg atatggaggg cgaacaccag
    54421 cacttgtctc tggaagaact tcagactatt catgccaata agactgggaa gttactagcc
    54481 tatcccttcc aagcggcagc tattatagct gaattgtcac ctgaaatgca ggtgaagctg
    54541 aaaactgtgg gtgaattgat tggacttgct tttcaagtca gagatgatgt actggatgtg
    54601 acagctagtt ttgaggaaat cggcaagaca cctcaaaagg atctgcaggc agaaaaatca
    54661 acctatcctg ccttgttggg cttggaagag tccattgcct tttgtaacca aaccctggat
    54721 caagctaatg aaaaattaga agaaattgcc cagcagcttc cctttgaaac agaatcgatt
    54781 gtaagtgtag tagaaagttt gagaatcaat ggctaaggaa agagtggatg tactagctta
    54841 taaacagggg ttgtttgaaa cgagagagca ggccaagcga ggtgtgatgg ctggtctagt
    54901 cgtagcagtc cttaatggag aacggtttga caagccagga gagaaaattc cagatgacac
    54961 cgaattaaaa ctcaaggggg agaaactcaa gtatgtcagc cgtggtggtt tgaaactgga
    55021 aaaggccttg caggtctttg atttgtcggt ggatggcgcg actacgattg atatcggggc
    55081 ctctactgga ggttttaccg atgtcatgct acagaatagt gccaagttgg tctttgcagt
    55141 cgatgttggt accaatcagt tggcttggaa attacgccaa gacccacgag ttgtcagcat
    55201 ggagcagttc aattttcgct atgctgaaaa gactgatttc gagcaggagc cgagctttgc
    55261 cagtattgat gtgagtttca tttcccttag tctgattttg ccagccttgc acagtgtctt
    55321 ggctgatcaa ggtcaggtgg tagcacttgt caaacctcag tttgaggcag gacgtgagca
    55381 gattgggaaa aatggaatta ttcgagatgc taagattcat cagaatgtcc ttgaatctgt
    55441 aacagctatg gcagtagagg caggtttttc agtccttggc ttggactttt ctcccatcca
    55501 aggtggacat ggaaatattg aatttttagc gtatttgaaa aaagaaaagt cagcaagcaa
    55561 tcagattctt gctgagatta aagaagcagt agagagggcg catagtcaat ttaaaaatga
    55621 ataaaaaaga aagacttgaa aaaattagaa gattggtgac agattatcaa atcggcacgc
    55681 aagaagaaat tgtagaacat ttgaaagaag caggtatcac tgccactcag gcgacggtat
    55741 cccgagatat caaagagtta ggtattgtca aaattccttt gagagacaac acctatgtct
    55801 acgaattgcc aaaatcaatc gtaaaaagtc tgcaactggc tgaagacaat atcgaatcgg
    55861 ctgaattgat ggataagatg atcaatctcc aagttattcc aggaaatacg gcttttgtaa
    55921 aagctcagtt aatcgagact tttgcagaca agatttttag ctgtttgact gatgatagct
    55981 cgattttagt cattgccaga agtggaagtc tagcagagga aatctttgaa caagtaaaaa
    56041 attggtaggt ctatatgtta cttgaaattt cgataaaaaa ctttgccatt attgaggcta
    56101 tttccctcaa ttttgaaaag gggatgactg tcctgactgg tgaaacgggt gcagggaagt
    56161 cgattatcat tgatgccatg aatatgatgt tgggagctcg tgcgacgaca gatgttattc
    56221 gtcatggtgc accaaaggca gagattgagg ggcttttctc agttgagaat agtcgtcttt
    56281 tacaggaaat ttttgatgag caaggtttag aattaggtga tgaaattatc atccgtcggg
    56341 aaatcttgca aaatggtcgt agtatcagcc gtgtaaatgg tcagatggtt aatctgtctg
    56401 ttttgcgaac tattgggcaa catcttgtag atattcatgg tcagcatgac catgaggagt
    56461 taatgcgtcc ccaactgcat atccagatgt tggatgaatt tggtgatgcc gctttttggg
    56521 acttgaaaga aacctatcaa acgagttttg atgcctatcg gaaaatgcgc aagcaggttc
    56581 tggaagtcaa gaaaaaccaa caggaacaca aggcacgtat cgaaatgttg gaatttcaaa
    56641 tggcagagat tgaggcagca aacttgcagg ctggagaaga cttggatctc aatcaagagc
    56701 gagataaact cctcaaccac aaaaatattg cggatacact gaccaatgcc tacagtatgt
    56761 tggacaatga agatttttca agtctggcca atgttcgttc agccatgaat gacatggaaa
    56821 gtgtcgaaga gtatgaccct gaataccgtg aaatttcaag ctctctgtct gagacctact
    56881 atgttttaga agacattagc aaacgtttgg aagctatcat tgaggacctt gattttgatg
    56941 gcaatcgcct catgcaggtt gagaatcgtt tggacctcct tcatactatt actcgtaagt
    57001 atggtgggac tgttgatgat gttttgcttt attttgccaa gattacggaa gaatacaatc
    57061 tcttgacagg caataatctt tcgtctgagg acatggaagc agagcttaag aagttggaag
    57121 tcaatcttgt caatttggca ggtcaacttg cttctgctcg tcataatttg gctcagcaac
    57181 tcgaagctga gattaaacaa gaactgcaag atctttatat ggaaaaagcc cagtttcagg
    57241 ttcgttttag taagggaaaa ttcagtcgtg agggaaataa aatggttgag ttttatattt
    57301 caaccaaccc tggagaagac tttaaaccct tggttaaggt tgcttctgga ggggaattat
    57361 ctcgtctcat gttagccatt aagtctgcct tttcacgtaa agaaggcaag actagcattg
    57421 tctttgatga ggtggatacg ggagtttcag gtcgtgttgc tcaagctatt gctcagaaaa
    57481 ttcataaaat tggtcagcat ggtcaggttt tggctatctc ccatttgcca caagtaattg
    57541 cgattgcaga ttatcaattc tttattgaga agattagtaa tgaccattca acggtttcga
    57601 ctgttcgtct cttgacggtc gaagagcgag tggaggaagt tgccaagatg ttggcaggtg
    57661 atgatgtgac agaagcagcc ctgacgcaag caagagaatt gttgagaaac agggagaaat
    57721 aagatgacag attattatgt aattggagat gttcacggaa aagctgggat gctggaagac
    57781 cttctcaaaa cctgggatgg tcagccccag ttgctctttc taggtgattt gattgacaga
    57841 ggtgaggata gtcaccgtgt tcttgaaatg gtcaaggact tggtggacaa tcaaggggct
    57901 atctgtttgt ccggaaacca cgagtatatg tttttgactt ggctagatga cccagaagaa
    57961 agttatgacc attatagtcg caatggtgga gatacaacca ttaactctat cctaggtcgt
    58021 cccttggatg caccagttga tggagtagaa gatgccaagc gggttgccgc tgaagcggca
    58081 gacttggtcg aattcattcg tcaaatgcca tttgtagtag agacagacaa gtatatcttt
    58141 gttcacgcag gtattgattt gaccttggac gactggcatg aaacaacaga ttataaaaaa
    58201 gtatggctta gaaaaccatt ccacggagct gaaaatcata ctggaaaaac cattgtcttc
    58261 ggtcatacac cggtctatgg tttattacag caagaacgcg gtacatctga gctttggact
    58321 acagaagatg gcaagattgg aatggatgga ggagctgttt atggtggtgt ccttcacggg
    58381 attgtcttta cagaccaagg aatgacagaa caccacttta tcgaaaatga cggctttgtc
    58441 gctgaagatt agtactccta gcagggtatg gtcttgtcaa aatatcaaaa acaatttata
    58501 ataaatagat accctgaaag gaagagaatc atgaacttag aagaattgaa aaaacgacag
    58561 gagaagatcc gtaacttctc tattatcgcc catattgacc acggaaaatc aactctagca
    58621 gaccgcattt tggaaaaaac agagacggtt tcaagtcgtg aaatgcaggc ccagcttttg
    58681 gatagcatgg agctagagcg ggaacgtgga attactatta agttgaatgc catcgagttg
    58741 aattacactg caaaagatgg ggaaacttat attttccact tgattgacac accagggcac
    58801 gttgacttta cctatgaagt ttcacgttcg ctagctgcct gtgagggtgc tattttggtg
    58861 gtcgatgcgg ctcaaggaat tgaggctcaa actcttgcca acgtttatct ggctttggat
    58921 aatgatttgg aaatcatgcc tatcattaat aaaattgacc tgccggctgc agatccggag
    58981 cgcgtgcgta cagagattga agatgtgatt ggtttggatg ccagtgaagc agttttggct
    59041 tctgccaagg ctggtattgg gattgaagaa atcctcgagc aaattgtaga aaaagtacca
    59101 gcaccaacgg gtgatgtgac ggcgccactt aaggccttga ttttcgactc tgtttacgat
    59161 gcttaccgtg gggttatcct ccaagtgcgt gtcatggacg gagtggtcaa acctggtgat
    59221 aagattcagc tcatgagcaa tagtaagacc tttgatgtgg ccgaagtcgg tatttttaca
    59281 ccaaaagcgg ttggtcgtga tttccttgcg actggtgatg ttggttacat tgcggcgtct
    59341 atcaagacgg ttcaggatac tcgtgtgggt gataccgtta ccttggcaac caatcctgcg
    59401 gcagaaccat tacatggtta taagcagatg aatcctatgg tctttgcggg tctctaccca
    59461 atcgaatcaa acaagtacaa tgacctgcgt gaagcgcttg aaaaattgca actgaatgat
    59521 gctagtcttc agtttgaacc agaaacatct caggcacttg gatttggttt ccgttgtgga
    59581 tttcttggac ttctccatat ggatgttatc caggaacgtt tagagcgtga gttcaatatt
    59641 gacctcatca tgacagctcc gtctgttatt tacaaagtta atttgaccga cggtgagtct
    59701 atggatgtgt ctaacccatc tgagtttcca gacccaacta agattgcgac cattgaagag
    59761 ccttatgtca aggcgcaaat catggtacca caggagttcg tcggagcagt aatggagcta
    59821 gctcagcgta agcgtgggga ctttgtgact atggactata ttgatgataa ccgtgtcaat
    59881 gttatctatc aaattcctct tgctgaaatt gtctttgact tctttgataa acttaagtct
    59941 tcgacacgtg gttatgcaag ctttgactac gaattgtcag aatatcgccc atctaagctg
    60001 gtgaaaatgg atattcttct caatggagat aaggtggatg ccctcagctt tatcgttcac
    60061 aaggactttg cctacgaacg tgggaaactc atcgttgata aactcaagaa aatcatccct
    60121 cgccaacaat ttgaagttcc aatccaagcg gctattggac acaagattgt cgctcgtact
    60181 gatatcaagg cccttcgtaa gaacgtactt gctaaatgtt atggtggtga cgtttctcgt
    60241 aagcgtaaac tccttgaaaa acaaaaagct ggtaagaaac gcatgaaatc catcggatca
    60301 gttgaagttc cgcaagaagc cttcctcagc gtcttgagta tggatgaaga ataagttatc
    60361 agaaatgcct ttccttgttc acaggaaatt tataagaatc aatagtaaag caggcttaga
    60421 tggtctgctt ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc
    60481 cttaccgta
//
LOCUS       DAVBCV010000006        51676 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBCV010000006 DAVBCV010000000
VERSION     DAVBCV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.006"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(54..362)
                     /locus_tag="VQ516_000362"
                     /pseudo
     CDS             complement(54..362)
                     /locus_tag="VQ516_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            complement(604..900)
                     /locus_tag="VQ516_000363"
     CDS             complement(604..900)
                     /locus_tag="VQ516_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510866.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(884..3043)
                     /gene="pfbA"
                     /locus_tag="VQ516_000364"
     CDS             complement(884..3043)
                     /gene="pfbA"
                     /locus_tag="VQ516_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2510867.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVISTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKESVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(4173..5399)
                     /locus_tag="VQ516_000365"
     CDS             complement(4173..5399)
                     /locus_tag="VQ516_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2510868.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(5442..6134)
                     /locus_tag="VQ516_000366"
     CDS             complement(5442..6134)
                     /locus_tag="VQ516_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2510869.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(6320..8071)
                     /locus_tag="VQ516_000367"
     CDS             complement(6320..8071)
                     /locus_tag="VQ516_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510870.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(8061..9806)
                     /locus_tag="VQ516_000368"
     CDS             complement(8061..9806)
                     /locus_tag="VQ516_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2510871.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(10012..10308)
                     /locus_tag="VQ516_000369"
     CDS             complement(10012..10308)
                     /locus_tag="VQ516_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2510872.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(10461..10917)
                     /locus_tag="VQ516_000370"
                     /pseudo
     CDS             complement(10461..10917)
                     /locus_tag="VQ516_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            11051..11878
                     /gene="xth"
                     /locus_tag="VQ516_000371"
     CDS             11051..11878
                     /gene="xth"
                     /locus_tag="VQ516_000371"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2510873.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11890..12033
                     /locus_tag="VQ516_000372"
     CDS             11890..12033
                     /locus_tag="VQ516_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2510874.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRSEENFLFLLK
                     SVN"
     gene            12033..12272
                     /locus_tag="VQ516_000373"
     CDS             12033..12272
                     /locus_tag="VQ516_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2510875.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12656..12751
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            13151..13732
                     /locus_tag="VQ516_000374"
     CDS             13151..13732
                     /locus_tag="VQ516_000374"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2510876.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13732..14994
                     /locus_tag="VQ516_000375"
     CDS             13732..14994
                     /locus_tag="VQ516_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2510877.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(15081..15947)
                     /locus_tag="VQ516_000376"
     CDS             complement(15081..15947)
                     /locus_tag="VQ516_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2510878.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(15934..16704)
                     /locus_tag="VQ516_000377"
     CDS             complement(15934..16704)
                     /locus_tag="VQ516_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2510879.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(16730..17584)
                     /locus_tag="VQ516_000378"
     CDS             complement(16730..17584)
                     /locus_tag="VQ516_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2510880.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(17741..18145)
                     /locus_tag="VQ516_000379"
     CDS             complement(17741..18145)
                     /locus_tag="VQ516_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2510881.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(18215..19696)
                     /locus_tag="VQ516_000380"
     CDS             complement(18215..19696)
                     /locus_tag="VQ516_000380"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2510882.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIVYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSADHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(19715..20893)
                     /locus_tag="VQ516_000381"
     CDS             complement(19715..20893)
                     /locus_tag="VQ516_000381"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2510883.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVFENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            21058..22065
                     /gene="galR"
                     /locus_tag="VQ516_000382"
     CDS             21058..22065
                     /gene="galR"
                     /locus_tag="VQ516_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2510884.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHDRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(22111..23148)
                     /locus_tag="VQ516_000383"
     CDS             complement(22111..23148)
                     /locus_tag="VQ516_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2510885.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAVEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLQSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(23149..23502)
                     /locus_tag="VQ516_000384"
     CDS             complement(23149..23502)
                     /locus_tag="VQ516_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2510886.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(23746..24636)
                     /locus_tag="VQ516_000385"
     CDS             complement(23746..24636)
                     /locus_tag="VQ516_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2510887.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGIPAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            24769..25305
                     /locus_tag="VQ516_000386"
     CDS             24769..25305
                     /locus_tag="VQ516_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2510888.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(25472..26278)
                     /locus_tag="VQ516_000387"
     CDS             complement(25472..26278)
                     /locus_tag="VQ516_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2510889.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(26623..28143)
                     /locus_tag="VQ516_000388"
     CDS             complement(26623..28143)
                     /locus_tag="VQ516_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2510890.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(28136..28864)
                     /locus_tag="VQ516_000389"
     CDS             complement(28136..28864)
                     /locus_tag="VQ516_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510891.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(28879..29643)
                     /locus_tag="VQ516_000390"
     CDS             complement(28879..29643)
                     /locus_tag="VQ516_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2510892.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVVDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(29647..30090)
                     /locus_tag="VQ516_000391"
     CDS             complement(29647..30090)
                     /locus_tag="VQ516_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2510893.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(30772..31068)
                     /locus_tag="VQ516_000392"
     CDS             complement(30772..31068)
                     /locus_tag="VQ516_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2510894.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(31090..32154)
                     /gene="pepA"
                     /locus_tag="VQ516_000393"
     CDS             complement(31090..32154)
                     /gene="pepA"
                     /locus_tag="VQ516_000393"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2510895.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            32555..33256
                     /gene="galE"
                     /locus_tag="VQ516_000394"
     CDS             32555..33256
                     /gene="galE"
                     /locus_tag="VQ516_000394"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2510896.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            33679..34638
                     /locus_tag="VQ516_000395"
     CDS             33679..34638
                     /locus_tag="VQ516_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2510897.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKEISESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLGFILLLWRGGRKDAD"
     gene            34628..35584
                     /locus_tag="VQ516_000396"
     CDS             34628..35584
                     /locus_tag="VQ516_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2510898.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLTLQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            35581..36333
                     /locus_tag="VQ516_000397"
     CDS             35581..36333
                     /locus_tag="VQ516_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2510899.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            36429..37394
                     /locus_tag="VQ516_000398"
     CDS             36429..37394
                     /locus_tag="VQ516_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2510900.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPAILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(37619..37861)
                     /gene="yidD"
                     /locus_tag="VQ516_000399"
     CDS             complement(37619..37861)
                     /gene="yidD"
                     /locus_tag="VQ516_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2510901.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(37861..38583)
                     /locus_tag="VQ516_000400"
     CDS             complement(37861..38583)
                     /locus_tag="VQ516_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2510902.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(38570..39139)
                     /locus_tag="VQ516_000401"
     CDS             complement(38570..39139)
                     /locus_tag="VQ516_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2510903.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKSQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(39158..39886)
                     /locus_tag="VQ516_000402"
     CDS             complement(39158..39886)
                     /locus_tag="VQ516_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2510904.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(39886..40626)
                     /gene="xerD"
                     /locus_tag="VQ516_000403"
     CDS             complement(39886..40626)
                     /gene="xerD"
                     /locus_tag="VQ516_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2510905.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(40617..41078)
                     /locus_tag="VQ516_000404"
     CDS             complement(40617..41078)
                     /locus_tag="VQ516_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2510906.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(41075..41596)
                     /locus_tag="VQ516_000405"
     CDS             complement(41075..41596)
                     /locus_tag="VQ516_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2510907.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(41572..42543)
                     /locus_tag="VQ516_000406"
     CDS             complement(41572..42543)
                     /locus_tag="VQ516_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2510908.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(42540..43334)
                     /gene="racE"
                     /locus_tag="VQ516_000407"
     CDS             complement(42540..43334)
                     /gene="racE"
                     /locus_tag="VQ516_000407"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2510909.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIYVEHVEL"
     gene            complement(43541..43789)
                     /locus_tag="VQ516_000408"
     CDS             complement(43541..43789)
                     /locus_tag="VQ516_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2510910.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(43931..45556)
                     /gene="treC"
                     /locus_tag="VQ516_000409"
     CDS             complement(43931..45556)
                     /gene="treC"
                     /locus_tag="VQ516_000409"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2510911.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(45734..47701)
                     /gene="treP"
                     /locus_tag="VQ516_000410"
     CDS             complement(45734..47701)
                     /gene="treP"
                     /locus_tag="VQ516_000410"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2510912.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGNDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            47886..48596
                     /gene="treR"
                     /locus_tag="VQ516_000411"
     CDS             47886..48596
                     /gene="treR"
                     /locus_tag="VQ516_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2510913.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(48629..49432)
                     /locus_tag="VQ516_000412"
     CDS             complement(48629..49432)
                     /locus_tag="VQ516_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2510914.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(49445..49873)
                     /locus_tag="VQ516_000413"
     CDS             complement(49445..49873)
                     /locus_tag="VQ516_000413"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510915.1"
                     /translation="MKTEIGLVLRSTGDNIPMSEANGVNVNTMKIVGYMISTGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPALQKRLKIRGLKLRNE"
     gene            complement(49882..50322)
                     /locus_tag="VQ516_000414"
     CDS             complement(49882..50322)
                     /locus_tag="VQ516_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2510916.1"
                     /translation="MNFVLSSLSEGLLWSIMAIGVYLTFRILDIADMTAEGAFPMGAA
                     IVVSQIQAGANPWLATLLALLAGMVAGLISGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVWF"
     gene            complement(50367..51272)
                     /locus_tag="VQ516_000415"
                     /pseudo
     CDS             complement(50367..51272)
                     /locus_tag="VQ516_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT        15174 a        11736 c         9478 g        15288 t
ORIGIN      
        1 attaaaaggt taagtaattt ttccaaaata cagcactcat atagacagta tcatcaatta
       61 tagccattgg gattttttga ttgccaattc catgtatctc tacacatgtc ttcaatcgat
      121 tttgttgtcc tccaatttaa ttccttatat gctttgtctg catttgcata ataagttgca
      181 acgtctcctg aacgtcttgg aactatttta taaggaatag ggatcttatt aacactttca
      241 aatgtattta caagttgtaa tacactagtg ccttctcccg agcctaggtt atagatataa
      301 acatctgttt tttcagatac tttttctaaa gcttttatat gtcctattgc taaatctact
      361 acttgaatct tttatctcat ttttcccttt ttcaaataag ttatctactc tatgatcagt
      421 tatttccatt tcgtttacta acatgacgca gtcacctagc atcatatact ccaacttttt
      481 ttctgaaagt ttagcaaata ttggtaagcg ataatatagt atattgaaac tagaatagta
      541 cacctctact tctaaaacat tgttagaaat cgatttgact gtcctgattg atttgtccta
      601 ttattatttc attttactat actctgttaa ttcatatgag tttaaaccga tttcatcttt
      661 aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt tacaattaac
      721 cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta tctcctcttt
      781 agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt gtgaacctac
      841 aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta tctttaccat
      901 aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt aagaatagtt
      961 ccataatctt gttcgaacca gtctttggta atttttgttt tacatctact atttctttag
     1021 atttattaat atgattttca gtttctctgc catctccaac tattttatag tttacttctt
     1081 ctgtcttatt atcttgttta ttgtcgatct tgtcattcat ttgtctatta tctttacttg
     1141 agttaaactc tccgttcttc tggttactat caattacatt atttgaatta gattgttttt
     1201 cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca attttgtaaa
     1261 gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca gattctttcc
     1321 catttgcatt agatttgatg aatgatatat acttaccgga taaattataa aattggttat
     1381 ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta ccaccgaata
     1441 tactattctc tgagactact aaattatcac taactcgtaa taattcaata tttggttgtt
     1501 ctgtttcctt agaattatta ttaattacat tttttgttac agtaatatct gatacttttc
     1561 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
     1621 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
     1681 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
     1741 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
     1801 cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
     1861 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
     1921 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
     1981 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
     2041 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
     2101 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
     2161 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
     2221 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac
     2281 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
     2341 ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg
     2401 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
     2461 aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
     2521 ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca ccataatctt
     2581 ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt
     2641 ccttataact cttattttca ttagtagata ttactgtaga ttcaactgtt ctgttgtcta
     2701 attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt aacgaattat
     2761 ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattt tgattaacag
     2821 ttggtgaatt atctaaatca ttagtgattg cattagaaga ctcttttgta gccatcggtg
     2881 aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca
     2941 atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca ttaggaacaa
     3001 aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct
     3061 taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc aaattgaatt
     3121 gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta
     3181 ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta tagtagactg
     3241 aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt gactgtcccg
     3301 atcgatttgt cctattctta tttcatttta ttatatctac aacctcaaag cagggttttg
     3361 agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat
     3421 ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttgtt gatatgttgt
     3481 aaattataaa tgaaagaaca aatatcttgt catacaagca ttcgccaggg atatttacat
     3541 gaatatcgct catatggcgg attgaaacta aaacactaca atatatttct taaatagacc
     3601 ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga
     3661 atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcgg gagatctaat
     3721 gatgtatagc aagcatcact aaacatatag aatatttgtg ttattataat gtcaatatgc
     3781 tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa
     3841 caatattctg aaaacaaaaa aagacgcgag tttccacaat ctcactcatg gaaactcacg
     3901 tctttcaaaa aatagggtga attaaaaact agaacaagac actagatttt ctaaatgatg
     3961 tctagtaagg attctaaatg ctacaccaaa ttgctcacat cttatttcaa accacgggac
     4021 tcccacaaga aaggaaaatc ctataaaaat tctctctctg aggagagaga ggcctcaact
     4081 acaggtgtaa actattatca attgaaaaca acagttttta cactcaatta gactttttag
     4141 ggacaggatc actttcgctt gactgtagtt tgtcattttt ttgataaagt tagcactttt
     4201 tcagaaactg ttttgaaagt ctcaatgata tagtctactt cttcatcgct taatttagta
     4261 tgaagaggga gggtaatttc attctcaaag aaggcatagg ccttaggata gttcgtcata
     4321 tcaaatccaa gattcttata ggctgtcaag agaggaagcg gtttgtagtg aacattactt
     4381 gcaattcctg ctttagccaa ttcttggatg atgaggttgc gttcttctag gcttgctcct
     4441 tctacacggg tgatgtagag gtggcgtgaa gattcgacag tttcagtctt gtgtgccaaa
     4501 ggatggatgc gagaacctgc aaaaccacta tcatagcggt ccacaatgtc cttacggcgt
     4561 tgcaacaaac ttggatagcg gtccaattgt accaaaccaa gtgaagccat gatatcggtc
     4621 atgttgcact tataggctgg tgtaacgata tcgtattccc atgaccccag ttgcatcttg
     4681 gcaagagcat ccttagtttg cccgtgaagg gaaaggattt ggaattcctt gtacatctct
     4741 tcgtcatcaa tcactggatt ggctttccaa gtcgcacttc caccttctgc cgttgtaaag
     4801 ttcttaacgg catggaatga gaaggaagta aagtcagcga tagaaccaga aggttgtcct
     4861 ttataagtag atcccaaagc gtgggcacta tcagagacaa tgacaatacg gttaaaggcc
     4921 ttttgccact tgcttgaagc ggtaaagaag tcacgttttt tctccacgac ttggaacaaa
     4981 cggtcataat cgcaaacaat ccctgcgagc tctactggga taatcacctt agttttctca
     5041 gtgatagctt gctcaagcag gtcatagtcc atctcaaacg tatctgcttg gatatccacc
     5101 atgacagggg ttgctcccac gtgcgtaatg acactacatg aagccgtata ggttatggct
     5161 ggaacgatga cttcatcacc aggtcccact tcaaaaacgc gtaaaatcaa ctccagagcg
     5221 gctgtcgcag agttgagaca aacagtctta ggtgtctgtg tgtaaagaga caagcggcgc
     5281 tccagttctt ttgttttagg acctgttgtg atccaaccag aacgcagggt atccgctact
     5341 tcagcaattt ctgcttctgt gatatcaggc ggtgaaaatg gaatattgta atttggcatt
     5401 gatttgctcc ttttatctgt actcattctc ttatgactac tttattttag taccttaaac
     5461 acggtttgaa acatgatttt gatgtcccca aagaaactaa actctcggag ataggcgagg
     5521 ttatagcgca tcttttcagg aagaacatgc tccacatagg cctgatcaac tgacagacct
     5581 ttctctgtca tttgactgat gatggtgtcc tcatccttgt agttgatgct ggctggagag
     5641 gtaattcctg cttgcaagag caaggttgcc atcatttcag tgctatactg ctctgtataa
     5701 cgtggcactt caggtcgtgt accgacaaag gacatctcac ctttaaggac attgaccaac
     5761 tgaggcagtt cgtccaaacg gacacgtcgg atgaaatttc caaccttggt aatacggcta
     5821 tcgttagcag aagtcaccag atttcctttt ttatccgcat ccgtcaccat ggtacggaac
     5881 ttccaaatct tgaaccgacg gttgtactgg gtcacacgct cttgcttgta aatcactggc
     5941 cccttgctat ccaacttgat ccaaatgctc aagatgagaa agatgggaga ggtcagaacc
     6001 agtaagacca aggccaaaac ccagtccaag caacgcttga aaatcagcga acccttcctt
     6061 ttagagacaa gctggtagta agactcaacc tcgcttgatt tcatttccac aggcaagtct
     6121 tcccatttca gcatgctgtt ctcctttgtt tttattatac tatttgtcaa cactttttgt
     6181 caacactttt cattatacca caaaatggaa aaggcggtga aaaaaatctg taatttgaag
     6241 gatttccttc tagtactcaa tgaaaatcaa agtacagctt tgagattgca gataaagctg
     6301 acatggttta aagagatttt caaagagtat aggccatggc ccctgcctgc aaactataca
     6361 tcttgtgata ggttcctccc agagccaaga gttcctcatg ggttccactc tcgataatgc
     6421 gtcccttatc caagacatag atgcagttgg cgtcttgaat agtagaaagg cggtgagcta
     6481 tagcaatagt tgttcggccc tgtctcatct tcgccagaga agcttgaacc aagctttctg
     6541 tttcagagtc aatattggct gtcgcttcat ccaaaatcag gattttaggc tggctggcga
     6601 ctgttctagc aaaggcaaga agctggcgtt gcccagtaga gaagctcgaa ccacgctcgg
     6661 aaacagggga gtcgtacccc tgaggaagtt cttgaataaa ggaatctgca tccacaaagg
     6721 ctgccgcagc ctgaacctgc tcatcactga tttcttggta catggcgata ttggacttaa
     6781 tagttccatg atagaggaag ggttcctgca agaccaaacc gatgtttttt ctcagctctt
     6841 cttgactgaa atccctgata tccacatcat ccaagagaac tctccctgac tggaattcat
     6901 aaaagcgcat gaggacattg ataatagacg atttccctga acctgtatga cctacaaagg
     6961 caatggtttc acccttatta acagagaaag aaatgtcatc cagaatcgga tgtttaccgt
     7021 catatgagaa acacacatgt tcaaagcgga tattgccttc ttggactttg gcttgcccat
     7081 tttcttgaag aggttcatag gtcctctcgt ctaccagggc aaagacacga cctgcagaaa
     7141 ccatagacgt ttgcagtgtt gaaaagtttt gcgtcacctc aatcaaaggg tcaaagaggc
     7201 ggttgatgta ctggataaaa gcatacatgg tcccggccgt tatcccgata gaaagaccac
     7261 gatagccaaa ataggccatc aagacagcat aacctagaag tttcaacaaa ctcatggcag
     7321 gtctcaaaaa gagggcatcc aaggctacag aacggttggc atagaccaag tgctcttggt
     7381 tgatttcatc aaattctgcc tgcaggcgct tctcttgatt aaaggcctgg attattctga
     7441 ttccctcgat gttttcagcc agcttgctat tgatatctga caagagactt ctggttttct
     7501 caatgatttt cactgatttt ttccgataga gattgaccaa aaggaaaatc aaggggagaa
     7561 agagcaagac taatgctgtc aaacgaaaat ccaataccaa catggtataa agggttgtca
     7621 gaaagatgaa aactgctgag ataaagctag ataaaatccc agaaaacata tcactaatcg
     7681 tctcggtatc gttggtcaaa cgagaaacga tagaacctgc tggcgtcttg tcaaagtaag
     7741 acatgcccag tttctccata ttggcaaagg catcccgacg aatatcccta acaatactgt
     7801 aagacacgcg cgcaaagaga agattgccga catactgaac tacagtttgt aggatgtaga
     7861 gaccatagta gaccagcaaa acggtaacgg ctagttggtt aagattgctg agatactggt
     7921 cgataaagtg ggaagccacg aggggtatga cacttttaat gaccgtcgtc gctaggagaa
     7981 aactgagtgc caaaaaggtc aggagtctat aaggcttgag ataagacatc aagcgcttca
     8041 atacagccca ttgttcttgt ttattctgca tcttcttctc ctttcatttc taactgctga
     8101 gactggtagg tttgggcata ccagccatcc atcgctagca agtcttcgtg cctgcctcgt
     8161 tcgataattt gaccattttg tagaactaaa ataaaatctg catggacaac agcactgagg
     8221 cgatgggcag ttataatggt tgtcttgtcc tttcgcgtct ccttgagatt gtcgataatc
     8281 gcatactctg tcttggcatc tacggcggat aaggaatcat ccaaaatcaa gatatcaggg
     8341 tttaaaatca tagcccgact catagccaac cgttgctttt gaccaccaga aagactgact
     8401 cctttttcac caatcagcgt atcaaatcct tgaggcatgt ctacaatatc ttggtaaact
     8461 cgggctagct tagtagcttc ctcaacctct gaaaggggca agttaggatt gccaaagcgg
     8521 atattgtcta ggattgaagt cgcaaagaga aactggtcct gaggaacata gcccatgaga
     8581 ctgcgaagtt ctgtcaaacg atagtcccga atatcgtgac cgtttagata aatggcaccc
     8641 ttatccacat cgtattcacg caagaggagc ttgattaagg acgttttccc agagcctgtc
     8701 tgcccaacca agcccagtgt ttgccctttt gccaaactaa agtgaatatc cgtcagtgtt
     8761 tcctcatttt caaaagcaaa gctgtcaatg gcatactcca aacgcccatt ttcaatacca
     8821 tccagaggaa actcagggtc ttgtacagga gattcctgag acaaaagatt ttcaatccgc
     8881 tggtaagaaa ccttccctcg ctgagtagta ttaaagagga agccgatggc cataagaggc
     8941 cagaccagca tatccaaata gctgataaag gtgactagat taccaactgt aatctgccct
     9001 tcctgaacca tcaaggagcc aaccaaaagc gttaaaacat aggacgaacc aacaaacaag
     9061 agaaccatag ggtcaaagag actatcatat ttcatggttt gcaggttctt ttggaaggtt
     9121 aattcattga ctgcctgaaa agacttcaac tcgtctgcct gataaccgaa agacttggtc
     9181 actttgatac ctgatacgga ctcctgtacc ttgttattga gttcagaaaa agcagcttgg
     9241 gattcgccaa aggccttatg agtctttctc cctaggcgac tagtcgcata ggccatgaaa
     9301 ggtaggggga gaatggcaac aagagtcatc tgccatgaga tgctaaagag catggtcaac
     9361 aaagtcacca gagccgtgat agaggcatcc accgcagaca tgacaccgcc acctgctaaa
     9421 cgagtcaagg cattgatatc attggttgcg tgtgccatca gatcacccgt ccgataggtt
     9481 tgataaaagg ctggcgacat ttttgtgaaa tgcttaaaca agcgagaccg catgatctgt
     9541 cccaagcaat aagaggtccc aaggatatac atacgccaca catagcgcaa atagtacata
     9601 ccaaaggctg caagtagcaa gtaaaatagg ctaagaagga ggtcctgctg ggttaattgc
     9661 cccgatgtga tggcatcaat gactcgcccc ataaccatag gaggaatgag attgagaacg
     9721 gaaaccaaga ccagggccac aatcccgact agataacggt gtttttctaa cttgaaaaac
     9781 caccagagtt tttgaataat ggacataaaa tccctttctg attgcaaatg gaaacctgag
     9841 gccaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgctcaaagc
     9901 actgctttga ggttgtagat agaactgacg aagtcagtaa cctacatacg gcaaggcgac
     9961 gttgacgcgg tttgaagaga ttttcgaaga gtacaagacc ccaggttttt cttattcata
    10021 agttacgact gtaacagcac ccttgtcata ttcagcaata aagatattgg ctacattgtc
    10081 atgcccttgt ttgctgaggt tatcaagcaa ccactcttcg ctacgaccaa tcgattccaa
    10141 gacgtctact tgaatcacac cgtcagtcac aactggatac ttaggatttt catctcccat
    10201 ttgcacaacg atgagttgcc cattttgctc ttgcacagct cgtttgactt gcttcatctg
    10261 gaaaatccct tggctacgaa gtttgagggc tacatccgat gcagacaaac caactgaacg
    10321 acaggcttat gggtcaatat tcccattttt gataaggaga gttggcttac catcaatcaa
    10381 acgtttcaca aagtgaacat tgttgttgag ctatttgaga gtcaagatca gtttgattaa
    10441 aatttcgata taattaagtg tcatttgttc acatccacca gttcaattgc gtctgtctta
    10501 tacaattcga ctttctctaa caggtacttg tctggctcac ttccgtttag ggcacgataa
    10561 tagcgatctc ccaccttgat aagtgcgcca tctgtgtttg tggaagtatt aacatggact
    10621 tctgacttgt ctactcccaa atctttggaa acaacctcga tgaaatgaag tgaagtttga
    10681 aatttattgt tagaaacttg attgttttga taagtcgtga tgctgaccaa aagcatagct
    10741 aaataaggcc agagctgaaa tcatgaccaa ctcacgaaat ttggttcctt ttttatcatg
    10801 gtatgccttg aaagcaaaaa aaactgtcac agctaataga acaatcccaa agccaaccat
    10861 gataccattt tgctgaccga tttggctgag tacatagtca taagagtaga atttcatgta
    10921 ttttcactcc ctttcataaa atcattctta attataaacg aaagagagta atttttcaaa
    10981 ccatacgttg gggaaataga tctttttttg gtataataaa atctataatc tgaatgaaaa
    11041 aggtaacttt atgaaactta tctcatggaa tattgattcc ctaaacgctg ccctaactag
    11101 tgactcagct cgtgccaaat tgtcccaaga agtcctacaa accttggtcg ctgaaaatgc
    11161 tgatatcatt gctatccaag aaaccaagct ttctgccaaa ggacctacaa agaaacacgt
    11221 ggaaatttta gaagaactct tcccaggcta cgaaaacacg tggcgctctt cccaagagcc
    11281 tgcccgtaaa ggctatgctg gaaccatgtt cctttataag aaagaactta cacccactat
    11341 cagcttccca gaaatcggtg ccccttctac catggacttg gaaggccgta tcatcactct
    11401 agaatttgat gcatttttcg taacccaagt ttacactcca aacgctggtg acggtctcaa
    11461 acgcttggaa gaacgccaag tctgggatgt caaatatgcg gagtatttgg ctgaactaga
    11521 caaagaaaaa ccagtccttg cgaccggtga ctacaacgta gcccacaatg aaatcgacct
    11581 tgcaaatcct gctagcaacc gccgttcacc tggatttact gacgaggaac gtgctggatt
    11641 taccaaccta ttggcaactg gatttactga taccttccgc catgttcatg gcgatgttcc
    11701 tgaacgctac acttggtggg cacaacgcag caaaacttct aaaatcaaca atacaggctg
    11761 gagaatcgac tactggctca caagtaaccg tatcgctgac aaggtcacta aatctgatat
    11821 gattgactca ggtgctcgcc aagaccatac accgattgtc ttggaaattg acttgtaagg
    11881 attttctata tggactacaa tgcggtcatt cccgagtttc ttgtatcgaa tatcgaacag
    11941 tcccggtcct tctactgtgg tttactgggt tttaggattg aataccaacg ttcagaagaa
    12001 aactttctct ttctcttgaa gagtgtcaac taatgttaga ggagggaaca aaggatcaac
    12061 tagctgaact gacctacccc tttggtcgtg gagtcaatct ctcctttggc ataaaggatg
    12121 ttccaaaact ctatcaaaaa gtgatggagg caaactatcc tatttatcgt cccttgacca
    12181 aaagaaagtt tcgtgtcagt gatccctata tctatcctca taaatttgca gttttggatc
    12241 cagatggcta ttttttaaga tttagcgagt agaataataa aaattgggaa aagtctttaa
    12301 aagttagaaa aaggcatatt gtcaggtgtc taaaaacttg atagtatgcc ttttatgatg
    12361 gaaaaatctt ctaatactct tcgaaaatct cttcaagcca cgtcaacgtc gccttaccgt
    12421 aggtatataa tactgactct gtcaattcta tccacaacct caaaacagtg ttttgaggtt
    12481 gtggatagct tcctagtttg ctctttgatt ttcatttagt ataaatgtta gcttacaatg
    12541 gagtttttgt tcaccctctc ttctgtttgt tcgtttttta aaccaaaaca ctaacaaaat
    12601 agttgatttc taaagttatt tagtgtaaaa taaaaacatt ggctcaagcc tatttaaaat
    12661 tgaatatcgt tttacttgtt tatgtcgtga attggcacga cgtttctaca aggtgccgga
    12721 acacctaaca ataagtaagt cagcagtgag atatggccgt ttttgccatg cctatttggg
    12781 ggacttactt agagattaag taggtctttt ttgaatattt ttaatatttc ccttaattag
    12841 tgcggacggg aggtgaaatt atcctgtgaa taatttcagg aatccaggga atttcatccc
    12901 taatttttga gcccctagct ctttcatttc taggcttagg ctaaaatagt tccccgaact
    12961 attttactct caaaaatccc gtcatagaca ataacaagat tattgtctat gataccacta
    13021 caacgggaaa tatttgataa gactcgcttc gctcgtaata gttttatcaa atatatagca
    13081 ttagcaaaag ggatcactat agaaacattt aaacaacaag taaattaact tttagacttt
    13141 aggaggtctt atgaaattat tagaagagcg catcctcaag gatgggcata tcttgggtga
    13201 taacatcctc aaggtagatt cctttttaac ccaccaagtt gactttagct tgatgcgaga
    13261 gattggtaag gtttttgcgg aaaaatttgc tgctgccggc attaccaagg tcgtaaccat
    13321 tgaagcgtcg ggtattgccc cagccgtttt tacagctgaa gccttaaacg ttcccatgat
    13381 tttcgccaaa aaagctaaga acatcaccat gaacgaaggc atcttaactg ctcaagtcta
    13441 ctcctttacc aagcaggtga ccagcaccgt ttctatcgct ggaaaattcc tctcaccaga
    13501 ggacaaggtt ttgattatcg acgatttcct tgctaatggc caagctgcta aaggcttgat
    13561 tcaaatcatc gaacaggccg gtgccacagt ccaagctatc ggtatcgtga ttgagaaatc
    13621 cttccaagat ggccgtgatt tgcttgaaaa agcaggctac cctgtcctat cacttgctcg
    13681 tttggatcgt tttgaaaatg gtcaggtcgt atttaaggag gcagatctct aatgcaaact
    13741 caagaaaaac actcgcaagc agccgttctt ggcttgcagc acttactagc catgtactca
    13801 ggatctatcc tggttcccat catgattgcg acagcccttg gctattcagc tgagcagttg
    13861 acctacctga tttctacaga tatcttcatg tgtggggtgg caaccttcct ccaactccaa
    13921 ctcaacaaat actttgggat tggactccca gtcgttcttg gagttgcatt ccagtcggtc
    13981 gctcccttga ttatgattgg gcaaagccat ggtagtggcg ctatgtttgg tgcccttatc
    14041 gcatctggga tttacgtggt tcttgtttca ggcatcttct caaaagtggc caatctcttc
    14101 ccctctatcg taacaggatc tgttattacc acgattggtt taaccttgat ccctgtcgct
    14161 attggaaata tgggaaataa cgttccagag ccaactggtc aaagtctctt gctttcagct
    14221 attactgttc tgattatcct cttgatcaac atctttacca aaggatttat caagtctatc
    14281 tctattttga ttggtctggt tgttggaact gccattgctg ctactatggg cttggtggac
    14341 ttctctcctg ttgcggtagc tccacttgtc catgtcccaa ctccactcta ctttgggatg
    14401 ccaacctttg aaatctcatc tattgtcatg atgtgtatca tcgcaacggt gtctatggtt
    14461 gagtcaactg gtgtttatct agccttgtct gatatcacaa aagatccaat cgacagcacg
    14521 cgccttcgca acggttaccg cgcagaaggt ttggccgtac ttctcggagg aatctttaac
    14581 accttccctt acaccggatt ttcacaaaac gttggtttgg ttaaattgtc aggcatcaaa
    14641 aaacgcctgc caatctacta cgcagctggt ttcctggttc tccttggact gcttcctaag
    14701 tttggtgccc ttgcccaaat cattccaagc tccgtcctcg gcggtgccat gctggtgatg
    14761 tttggttttg tatctattca agggatgcaa atcctagccc gagttgactt tgctaacaat
    14821 gaacacaact tccttatcgc agctgtttca atcgctgcag gtgtcggtct caacaacagt
    14881 aatctctttg tcagcatgcc gacagccttc caaatgttct tctcaaacgg aatcgtcgta
    14941 gccagcctac tcgccattgt cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa
    15001 gaggtgcgag cctctttttt gtttatcaca aattgattat aaatacaatc aatcccaatt
    15061 cccatctaat tgaattggac ctatttcaaa ttctttaaaa tattatgttc caaatcgtta
    15121 atattatata agaatggtaa aatatcaaat gtttctcgta aattattctt agccttatac
    15181 caaccttgtc catcagtaat ccacataaac tcaacattag gaatagcttt aatttcttca
    15241 gcaatcattt tgtaagatct agccgtctca tttaattttg aaccacttcc tgaataaaaa
    15301 ttaacctcaa tcagataaag cacttgttca ttttttatga caaaatcaaa acgtttcaca
    15361 gtattcccat cattcgtaat agcagataaa tcgacagaaa aaatttcctc tatttcattt
    15421 tgttcaatct ctttaaataa attctctcct aaaatatacc cttcagcttc aagataactt
    15481 tgaacaatat tttccatcgc atctccagtc cgatttttgc gcccattagt atccatacca
    15541 acctcaacac ctgtaacgta gtcaactaaa tttgatacta aatgattttg caataaatca
    15601 aatatacctg atttttccaa aaacatagta tattcttcaa tactatagtt tcgtttacta
    15661 aaatcaaaat agaaatcttt tatcggatcc ttaacaataa tcgtatctct caaacgttta
    15721 gcaataagta gaggcacaac tttcaaaatt tctggataat tttgatataa gtctaaaaaa
    15781 tcctcttgta tatttttact accaataaga ctattcataa tatttagagc aacttttata
    15841 ctagatacat ttttataaac cttgggaaaa tctgtataat aagtccagtc agctactgtg
    15901 tcggtcatcg ttgacaacca ctcatcaaaa tttctagttt gtttcatttt ctgcctccaa
    15961 cctcttcctt gcaattttta aatattcttt ttcagcatcg atacctataa atcttctacc
    16021 taaccgcttc gcaacaacac ccgtagtgcc actaccaaca aatgggtcta gaatatagtc
    16081 accctcttta gtagaggcta aaataatgcg ttctaacaaa tactctggtt tttgagttgg
    16141 atgtttccca gcccattttt caactttctt tgttaaagaa cccgtccaga catctttcat
    16201 ttgttttcca tcattcaatt ctttcattaa atcataattg tagtaatgac gagctttttt
    16261 atcatttttt ctggcccata aaatggtttc agtagaatgg gtaaaataac gacaagataa
    16321 attgggggca gggtttgttt tctgccaagt aatattattc agaattttaa aaccttcttg
    16381 ttctaatgcc attccaactg agtatatgtt gtgcaaacta cctgaaatcc ataccgtccc
    16441 attaggcttc agaacttctt ttgctaggcg aatccattta cgattaaact catgtttttc
    16501 ttcgaatgaa gaaattttat cccaatctcc tttatcaaca gaaacaactt gtcccccaga
    16561 attagatatt ccaccattac ttaaaaaata aggtggatca gcaaaaatca tatccatact
    16621 ttctggtttc atttttgata agaacttgaa cgtatctgaa tgaacaagaa tcatcttatt
    16681 tttattgtag tatggttttg tcataagaac acctccatac ttatattcgt tatttttcat
    16741 aatttgtgac tataatttca gaaatttttc ctcgacttga agattttgct ccattagttc
    16801 gggtagcttc aacataatgt atattaaaat ccttatacaa ctcctctact aaagcactag
    16861 aagaatttga taacataaca taagctcctg tatcactcaa tctcttaaag gcatctctta
    16921 atcttacttg atctgcaaaa gagaatccct catgagtata agacgtaaat gcactcgtct
    16981 cagacaatgg aatatatgga gggtcaaaat acacaaaatc tcctgttcga acatctacaa
    17041 ttgccttttc aaaatctccc actttaattt ctagttgatt gttatttaga taaactgaaa
    17101 tagcagatat caattcctca tcaacaattt taggattctt ataacgtcca tatggaacat
    17161 taaattggtt cttagaattc acacgatata gaccattaaa gttcactctc aacatatata
    17221 gaatacgtgc agctctttgt acttcggaca tcatatctat tctttcatca cgatctgcag
    17281 aacgtaaatc taaataatat tcttttgaat tatattcctg atgaactttc aaaatttcaa
    17341 tcaattcttg aggattgtcc ttaatttgtt gatagcaatt tattagttca gcgttaaaat
    17401 cattaataac tgcatcttta ggagccaaat caaaaaataa agctccacct ccaacaaaag
    17461 gttcgaaata cctgttatag gttttaggca ttaattctct aataacaggc aataattgtc
    17521 ttttaccacc tgtccatttc gtgaacggtt gtaaagtaac tttctttatt tcttttatct
    17581 tcatgctttt aattatacca tagaaattaa aacacaactc ttgtattttc taccacatta
    17641 ctaaattttc tattttagaa ttaaatcata tcaatataaa aaaatgagta aggcaaatct
    17701 tataaattcc aaaattcacc tcactctatt aaattactct ttatatcctt acccgtcttt
    17761 cctcagacct ttggcctgtg acaaacatag taaaggttgc tttgcagaca tttctgccct
    17821 cttgattggt gatatccaca tccatcacac aggttgtacg accttgatgg acacattctc
    17881 ctttaatggt caatacatcg tcgagttttc ctgccttaag gtagttgata gaggattgga
    17941 gtgtcacccc atcaagtccc agcgagataa ccaccaaacc actgatttgg tcacatagtg
    18001 tgaatagata gccaccatgg gcattgccat agtagttgag cgacgagttc actactttgg
    18061 tcgtcaccac aacgtgacca tctctcattt tttcaatttc gtaattttca aaggcagata
    18121 tagcgtcaaa atgaaagtct ttcatcgttt cctcctgata tttcaaatga cactatgata
    18181 ctacttttta taggactgtg caaggagaaa gctcctagtc tagtaaaatt ccgacatgtt
    18241 ccacaaagcg cataaaggct gtctgccctt gttctgtctg cttgtagact cctgcatcct
    18301 caagtacacg cgcaaagata gcacccacag agtccttgac gattgcaagg gctttttctt
    18361 tatccgttag gtctggatgt tgggatttga gttggtctgc ccactcctga tgataatcgg
    18421 caactgtaac agcttctcct acaagatagc tagcgacttg ctctacttct tctttcagac
    18481 gtggtggcaa gattgccaag cccatgacct caatcaagcc gatattttcc ttcttgatat
    18541 gttggacatc cttgtgggga tgatagatgc catcaggatg atctgctgaa gtctgattgt
    18601 ctcgcaagac caagtccaac tcaaactgtc catcacgctt acgcgctatt ggtgtgatgg
    18661 tgtgatgcgg tgtcccgtct gtctctgcta aaatctgcac actagaatct gaatactgac
    18721 gccattcctg gaaaatctta tcagctaagt taatcaaatc ctctttggaa tccgaagtca
    18781 agcgtaggac agacattggc cacttgataa ttccagcctt gacctgctca aaaccagcaa
    18841 atcggaaggt cttttgcaag ggagccaatt ccataggaaa tacgtgacgg cctccctgat
    18901 aatgatcatg agttagaata gagcccccca caatcggcag gtcggcatta gatccagcaa
    18961 aatatcctgg aaactgctct acgatagcca acagacgttc aaaactctga cgactaatgg
    19021 ccatgggacg atgctggcca tctaaaaaga tacaatgctc attaaagtaa gcatagggcg
    19081 aatactggaa accccattcc tgaccaacca tttcaaaacg gataatccgg tggttgctgc
    19141 gagctgggtg gttgacccga ccatggtaac cctcattctc taaacaaagt tgacactgag
    19201 gataattact agcttgcacc aacttggcta ccgcaatctc tttcggatcc ttttcaggct
    19261 tagagagatt gatggtaatt tcaagttctc cataatcaga tggaacacga taaacgatat
    19321 ttttagcaat ggccttgagt ttgatgtagt catttttctg actgagttga taaaaatcct
    19381 ctatcgcttg ctcaggagag taggcatagg cttcccagaa gtcacgattg acctgactcg
    19441 gacaaggagt caccaagtcc atcaattcag taccaaggat ttcacgcgca gtctgactat
    19501 cctcaatcgt ctctaatcga acggcttcct caactagctg gtccttgagg tcaatcaatt
    19561 tatccagatt ggtctcaact tccaaaacac cttctcccac tcgtgccaaa acacgattgg
    19621 tcaggtagat tcgatccatt tcctcaaatg aactttcaga aatgacatgt gttacaaatt
    19681 tatctactaa ggtcactata gagcctcctt ttgactagtc aaggacgcga gtgccacctg
    19741 caacttcagc gatatagaag cttggagcgt atccaactac ttcctcgtag tgtttgccta
    19801 cagcttcctt aaaggcctca acagtatctt tttgaaccaa ggcaatggca cagccaccaa
    19861 aaccagcccc tgtcatacga gcaccgagaa ctccttcttg tgcccaagct gtgtgaacaa
    19921 gagtatccaa ttccaaacca gttacttcat aatcatgctc cagagaaacg tgtgacgcat
    19981 tcatcaaacg accaaatgtt tccaaatcac ctgcttgaag ggctgcttga gctttaaggg
    20041 tacgttggtt ttcaaacaca gcatggcgag cacgtttcaa acgattttca tctttaatca
    20101 gatagctata ttggtcaacg gcccactcgt ccaattcacc cagggtctga atatccaagg
    20161 caacttgcaa ttcttccact gctttttcac actcagcacg acgttcattg tatttagagt
    20221 ccgccaattc acggcgtttg ttggtgttca tgataacaac gacattgtcc ttcaaatcaa
    20281 gtggcaccaa gtcgtattct aaagtattgg tatctaagta aatagcacgt tggtcagccc
    20341 ccataccaat agcaaattgg tccatgatac cagagttgac tccgataaag ttattttctg
    20401 tttgttttcc gattttaacc aaatcgagac gctctagttt taaatcaaag agatgctctg
    20461 ccacgactcc tgtcaagagc tccaaggatg ctgaagaaga caaaccagca ccatttggga
    20521 tattcccata aacataaaaa tcaaaacctt tgtcaatcac gtgcccagct tcttgcaaga
    20581 aatgaaggac accttttgga taattggtcc agttgtgctc tttttcaaac ttgaggtcag
    20641 cgagaggcac ttcgataatg cccttgtcct caaagttagc tgagtagaaa cgcaagactt
    20701 ggtcgtcacg cttacgagct gcaccgtaag ttcccaagga aatagcagca ggaaaaacgt
    20761 gcccaccgtt gtagtctgtg tgttcaccaa tcaaattaat gcggcctggt gaaaagaagg
    20821 tttggtctgc ttcttgacca aaaacagcaa gaaagtcttt gcgaagggct tcagtagtaa
    20881 gatgttgtgc catatgaatt ctcctttgac tgtccgttaa gccaccttaa cgtataatcg
    20941 ttttctttct attgtatcca aggaattttc caagatcaat cctttttact aaaattttag
    21001 taaaatatca gtaaaaagaa gaaaaacatg atatactaac ctaatcaagg aggatttatg
    21061 gctaccttaa aagacattgc acagctagcc tctgtctcta tcgcgaccgt atcccgcgtc
    21121 ctcaatcgcg accagagcct atctgttaca gaagaaacca gacaccgtat tttaaccgtt
    21181 gctgaagagc tgggctacac caagcacctc aagacaggcg agtcccacaa acccaagcaa
    21241 aagattgcca ttatccaatg ggtcagcgaa caaggggagc tggacgacct ctactactac
    21301 cagattcgcc taggaataga aaaaagagcc caagagttgg actatgatat cttgcgctat
    21361 tttaatgacc atccttttac cttaagcgag gaagtgattg ggattctctg cattggaaaa
    21421 tttagccgag ctcagatttc tgcctttgaa gaataccaaa aacccctggt ctttctagac
    21481 agtgatacac tttctctggg gcatacttgt attattacgg atttttacac tgccatgaaa
    21541 caggttgtcg attatttcct cagccaagga atggaccgta tcgggattct aacaggcctt
    21601 gaagaaacaa ctgaccaaga agaaatcatt caggataagc ggctggaaaa tttcagaaac
    21661 tacagtcaag caaaaggaat ctatcatgat gaactggtct ttcaaggaaa ctttactgcc
    21721 cagtctggct atgacttgat gaaggaagcc attcagaact tgggagacca actcccacca
    21781 gcatttttcg cagccagcga tagtttagct atcggtgccc ttcgtgccct ccaagaagct
    21841 ggaatcagcc taccagaccg tgtcagcctc atttccttta acgacaccag cctgaccaag
    21901 caggtttatc ctcccctctc cagcatcaca gtttatactg aagaaatggg gcgagcaggt
    21961 atggatattc ttaacaagga agtcctccac gatcgcaaaa ttcctagcct gaccatgctg
    22021 ggaaccagac tgacattaag agaaagtacc ctaaatcaag aataggataa cataaaaaac
    22081 gaatagagtt ctaaaactcc tattcgtttt ttattcgatt acaatcatag acttaatggt
    22141 cttacgttca tccatatctt tgtaggcttg gtcgatatct tccagtttat aacttgaagt
    22201 aaagacgcga cctggattga tatcaccatc aaggacggct tttagtaaaa attgcttatc
    22261 gtatgttgta gcagaagctg ccccacctgc tacagagata ttttgcataa atgtcgaacc
    22321 aagagcacga ttattatagt gtgggactcc tacaaagccc atacgccctc cattatgaag
    22381 aacacctagc gcctgttcta tagcagcctc cgtaccaaca cattcaagtg ctgcgtctgc
    22441 tcctccaccg aggatttcac gcaccttggt aattccttct tgaccacgtt ctgcaacaac
    22501 agctgtcgca cctgactcca tagccatctt ttgacggtct tcatgacggc tcataaggat
    22561 aatttgtgat gctccacgca tcttagccgc gatgacagca cattgaccaa cagccccatc
    22621 accgataaca acaaccttgt cccctttttg aacatttgca acacgcgccg catgatagcc
    22681 tgtcggcatg acatctgcaa gagtcaaaag ggactggagc atcccttctg tatagtcaga
    22741 aggttgacca gggattttaa ccagcgccca gtttgcatag tggaagcgaa tatattctgc
    22801 ctgaaaatca ccccccaaat tattgccaat atgattgtcg caagaaccgt caaatccagc
    22861 aagacaggca tcacactcac cacatccatg tgtaaaaggg acaatcacaa aatcacctgg
    22921 tttcaccgtc gtaatggctt ccacagcttc ttcaacaatc ccaatcgctt cgtgtccact
    22981 atttttgtgt ccagctttcg tttctggatt acggtacctc cataaatctg aaccacaaac
    23041 gcacgcacga accacacgaa taatcacatc atccgcttct attatttgcg gacgttcaat
    23101 gctagcaagt ccaacctgac ctgcctttgt atatactgct gatttcattt aaaattttcc
    23161 ttccttataa agtttaattt tgagatttaa acgatttaaa gctgtctgta gctgagacaa
    23221 gcgctcctct aatttttgct tttcctcttc taaaatacca agcctctcct ctctcgtttc
    23281 atctcccttt tggtagagcg acatatagtc aactaaacta tctacagaga cacccgccga
    23341 acgaaaacac ttaataaatt ccagcgcttc gatatcctga tcttgaaaat cacgaatccc
    23401 agtagcagta cgagtaatcg gtggcacaag accaacccgt tcataatacc gaatcgtatc
    23461 cgctgaaatt cccaacaaat cactggcaga tttaatattc acacgctaac ctcctctcat
    23521 ttcattcact gaattcacgc tattctaact cacttatctt attatataac tttgagttga
    23581 ctccaagtca agcattttgt atctaatcga aaaattttta tcattaacaa tcatatatct
    23641 ggagtttata caatgctctt ctcttaccaa tttttgaaat atttaaataa taaagaaatt
    23701 actctattcc aaataagata gtacccaagt atttttcact tttttctaat gttgatgctc
    23761 ataactccgt tccaagtcac acacctttcg cttatgggtt tggtgagttt ctaggtcagc
    23821 atcaatttca atggtaatat tttgaaaacc acaatctttt aggaaaattc gaatagactc
    23881 tttacaagtt tccatatgtt ccatttcttt tagacaaaca tggacaatgg catttttttc
    23941 caaagcatcc atagtccaga gattaagctg attaaggctg gccacattgt ccaatcgctc
    24001 caggccactc tttacttgct tgatatcaag accttctggc acagcatcca agaaaatctt
    24061 gagtgtagac caaaaacgtg gaagggcttt tgaaagaata aagaaagaaa tgacaaggga
    24121 caaaagagga tctaggatat accagtccgt aaatcgaaga acaatcgcca tcaggataac
    24181 agctacccac cctagcgtat cttccagaaa atgcagactc agaatagact cattctttgt
    24241 ctttccctta ccaaccacca gactcgctaa cagattgata gtaatcgcaa taattcctaa
    24301 ccagagaatc ccctcatcat tgactggttg cggatgcaaa atcttcgtga cattttccaa
    24361 aatgactaga acagagcccg ttacgagaat cacagctgtt accaaggctc ctagcaggct
    24421 aaaccgctta tagcccaagg tgtactgatt gtcttcttca cgattggaga ttgtttctag
    24481 aaaagctggt attccaattg caatcgcatc tcccaagtca tgcacagagt cagcaagaac
    24541 agcgctagaa ccaaatactc cacctgcaat aaactcaaca atggcataag tcaaatttaa
    24601 gaaaaaagcc acccaaacag catattttgc cttcatattt ctcattcctt tgttataata
    24661 gatttatgaa caccttgttc attatcatta tccactaaaa ctcaaagaaa ggatagaagc
    24721 aaaacgtcag ctttattcag ttctgaacaa tttgccttaa gtgtccatat gactaacatt
    24781 gaccgccgta tcagcaaaac caaaaaagcc atctatcaag cttttataca acttttgaat
    24841 gctaagggct acgaggccac tactgttcag gatatcattg atctcgcaga tgtgggacga
    24901 tccacctttt actgtcacta tgagagtaag gagcttcttc tggaccagct ctgccgctac
    24961 ctcttccatc atctttttga aagagagcaa gccatttcaa ccgaggacta cctcgcccac
    25021 ctctttctcc attttcataa aaaccaagac catatcacca gtctgctatt ttccaaaaat
    25081 gactacttcc tccgtcaact ccataaagag ctagaacacc atgtctattc cgtgctagct
    25141 gataatttaa aagaagccca ccctaatctg cctacttctt acctccaaca cttggtcatg
    25201 tccaacttta tcgagacatt gacctggtgg ctcaaaaaag gtcaagattt cacagaccag
    25261 gaagttgtcc aattttatct agaccttctc attcctaaaa attgaatata gagtaaagct
    25321 tcagttgtct tatttctagg ttactgagtt ttttatcttt tcaacaacaa aagaggaccc
    25381 gccgatcctc tttttcatac tataaatcct tgattatcaa ctatatctgt tttaatcgaa
    25441 atctcaaaac agcactttca aacatctttt cctagttaag taaatcagta ttctgcttag
    25501 ctgccttgct ccattgatac caaccaacta gactgttaat gagataaatt agatatttcc
    25561 cttgaatttg caggctttct ccccaccaga gatagattga aaagacattg gtagccgccc
    25621 agaatatcca ctgttcacgg taaacagctg tcatgaggat ttgccctacc ccattggttg
    25681 catctgtgat tgaatcacga tagggacgat tggcaccaat agactgataa atgaagccaa
    25741 aggccaacca ccaaagcaca ctaatggaaa gatactttgt ccagcccttg ccgtccagtt
    25801 tacgcgcgac aaactcctgc ttttccttct taaactgtgc ctgataaatc caaactagaa
    25861 gtccaattgg ctgcatgact gtgaagtaaa gtgtcgtcag cacctcacca taaaagcctt
    25921 tctgtagggc caaaataagg taaataacag agttaatcaa gccaaaaaga taattacttg
    25981 ctcgaccttc cgatacaaag attacacaga taatccctgt caagctacaa atcatcccaa
    26041 tccagtcaac aatacgatgt tcgtaaacca actccagcca gagaggaaaa cttcctaaaa
    26101 ccagcaaata aatccactgg gcaaaactac gatgggcaaa gaggtcatcc cagatagcct
    26161 tcatagttcc tgaaaatcct aaatcagcca tagccgcaac catacgacgg taaccacctg
    26221 acatttcacc tagggttgtt ttgatatttt caattttctt ttgcaaataa gtatgcatca
    26281 tttctccttt tgtttttaaa gagccgtgtc tggatagact ttcggacgca acgctctatt
    26341 agataatgaa ctgcctatac acaagatttc taaccttagt cgacatgagc tgaaacctct
    26401 tatttgttaa gtagttcaca aaatattata cacctatttt atgaatagtc aactgtcttt
    26461 acagtaaaat tttagaaaat catgaaaatt ttctctttct ttccatttta agtgacattc
    26521 agtcattctc acatcaaaaa agcccagacg aaattgtctg agcattcttt tatctagtcg
    26581 tttaaggaag ttgagttcag tatgtttaaa gtctctgtcc catcatttct tcaacaaacc
    26641 ttgttcttgg agaaactcct tggctacttg ctttgctgac ttgccttcaa caccgacttg
    26701 gtagttgagc tggctcatct ggctttctgt aatcttacca gccaatgtat taagaactct
    26761 ttccaactct gggtgtttct tgagaagagc ttctttcatg agtggagccc cttgataagg
    26821 tgggaagagt tgcttgtcat cttccaagac ctgtaaatca taacgctcca attccgcatc
    26881 agtcgaatag gcatccgtga tttgaatatc ccctgactga atagcctgat agcgaagggc
    26941 tggctccatg gtcgctacat tgagattgag accatacatt gattgcaagc ccttatttcc
    27001 atcttcacgg tcgttaaact cgagtgtaaa acctgccttc aactgccctt ccactttttt
    27061 caagtctgaa atggtcttca agccatattc ttgagcaatc tttttcggaa cagctacagc
    27121 ataggtgttt tgataagaca tgggtttgag ataggctaga tgatcctgct tagcaatgcc
    27181 atcacgcgcc acctgataaa cctgttctgg ttcatgactc accttgggtg atggttgaag
    27241 caaactttca gtcaccgtac cagtaaattc aggatagatg tcaatatcgc cttttttcag
    27301 agcttcataa aggaagcttg tcttcccaaa attcggttta acagtcgcag tcatgctggt
    27361 attttcttca atcagcaact tatacatatt ggccaaaatt tctggttctg gacctatttt
    27421 cccagcaata accaagtttt ccttctcttt ttgaaccaaa agagctggac tataagacag
    27481 acccagtaat aaagccacca aggcaaaacc tgagaaaatc gtccgtaatt ttgctttttc
    27541 catcactttt agtaggaagt taaaggcaat ggctagcact gcagaagaaa gtgccccaat
    27601 caaaatcaaa ctggcattat tacggtcaat tcccaaaaga ataaaggaac ctagtccccc
    27661 tgcaccaatc aaggccgcca aggttgccgt accgataatc aaaacagctg ccgtccgaat
    27721 cccagacatg ataacaggca tggcgagtgg aatttcaaat ttcttgagac gttcccatct
    27781 ggtcatccca aaggcaatcc cagcctcttg caggttcgga tcaattccct tcagcccagt
    27841 gatagtattt tgcaaaatag ggaaaatcgc ataaatcact agagctgtca aagccggcaa
    27901 ggtcccaatt cccatcaaag ggataaagag ccccaacaag gccagagacg ggatggtctg
    27961 gaaaatacct gcaatctgca agacccagtc ggccagcttc tcatgatagc gaagaaaaac
    28021 agccaaggga atcgcaagca aaatagctag taacaaggtc aaaagcgaca actgcaaatg
    28081 ttgagataga gctgtcaacc aatcactaaa acgatcctga aaagttgcaa ttaaattagt
    28141 catgaacact acctccaaac aagtctgcta caaagtctgt tgcaggcgct tttaaaattg
    28201 tctcgggatt cgctacctgg cgaatttctc catcctgcaa gacagcaata cggtccgcca
    28261 acttcaaggc ttcatccgta tcatgggtta caaaaatcgt tgtcatccca aactctttat
    28321 gcaattcttt tgtcagaacc tgcaactgtt ttctcgaaat agcatccaag gccgaaaagg
    28381 gttcatccat gaggaaaatc ttgggctgac caatcatagc tcggacaata ccgacccgtt
    28441 gctgttctcc accagataat tcactaggta agcgatgccc atactcggct actggtaaac
    28501 caaccttagc caaaagctct tctgttttct tcgtaatttc ttccttgctc caccccttca
    28561 tttcaggaat gagagcaata ttttccgcaa ctgttagatt tggaaaaaga gcaatagcct
    28621 gtaaaacata accagtagaa agacgaagtt cacgctcatc atagtctttg atgcgcttcc
    28681 catccatata aatatttcca tcagttggtt ccaaaagacg gttaatcatc ttgagcatgg
    28741 tcgtcttacc tgacccagaa ggccctacta aaaccataaa ttccccatcc tcaatctgta
    28801 agttgacatc tctcaagaca tccttttctg tgtagcgcag tgctacattt ttgtattcaa
    28861 tcattctttg tcctcaattt aaaacttccc tcgattggtc aagtcttcta ccttaggcat
    28921 aacttcctta ttatcccaat gctccacaat tttcccgttc tctaaacgga agatatcgta
    28981 ctgggcataa gcaacgccat caatctgagt ctgaccatag ctaaccacat agtttccttg
    29041 tcctaagagt tggaaaacaa agtcaaaagt gacactatat tcagccacat agtttttata
    29101 agcagcactt ccttgtccaa tatcatgatt atgctgaatc aaatcgtctg ccacataatc
    29161 actccactgc tctagctccc cattttggaa aatttctgtc aagaaacggc gaaccagctt
    29221 tttattttct gctttcttgt ccaaatcctt gatttcaaaa tctccaaaaa tttgatctag
    29281 ttggtcattt tcaggtgttc gatagtagtc aacgacatcc caatgctcaa caatacgacc
    29341 attctcatcc gcacggaaag tatccgtcgt cacccattga gcttctccac cattcagata
    29401 ttgatgaaca tgaacaaaga ccagattgcc atcctcaatg gtgcggacaa tcttaatctg
    29461 acgctctgga tgacgctcaa agaaatctgc aaagaaggct gcaaatcctt ctttcccgtc
    29521 aggaacacct gtcgaatgtt ggatataggt atcccctaca gactgggctt gagcctcagc
    29581 aactcgcccg tcttgaatgg catggatgta taggttgtga gcatttttca cttgttgtga
    29641 catattctaa acctcatttc ccttctcttt cagattcgcc aaaattcttt cttgaaaacc
    29701 ttcaaattgg tgaatttctt cctctgaaaa tcctttgtaa aagatagtat ccaatttctg
    29761 actgacacga tgccccactt ctttctggaa cttgcctaac tccgttaaaa ctaaatactt
    29821 cttacgcttg tcttttccac acggactaac aattacaagc ttttgttcct ctagcttttt
    29881 tatcatagtc gtcagcgtat tattcgcaag cccagtcgca agcgcgatat ctgtcgcagt
    29941 tgcgcagcca gtttcactat tccataaaac cgctaaaatc ttgccctgtt cacccctata
    30001 aagagcctca ggatcttgac tcagtaactt ttgaaaaatc cgcccattca acaaacgaat
    30061 atgatgggct agcaaatgac tatctttcat aacacctcca atttatttcg atatcgaaat
    30121 gaataaaata attgtaacac tcatcgttct aactgtcaac tatttcgatt tggaaataat
    30181 ttttgataat tatccacacc accatactcc ggctcaacta acttttaacg agagtttcta
    30241 aactccttcg tcctccagtc tacaaaagcc ttccattcgt actatcctat attttatgag
    30301 gggacacatt tttcctatca gaccatttat tttaaagata gaagtaaatc ataattgctt
    30361 ccatctgttc ttttatagta tattgaagtt agactagagc actgtatctt ctaaaacatt
    30421 gatagaaagc gatttgaatt tcccaatcaa tttgttcgta tttatagcat tttgaaacta
    30481 gaataggaca ccatgactgc taaaagattt ctataaattc atttgatttt cctaatcaat
    30541 ttgttcatat cttatttcat tccgctataa tttcacctta ccctatcttt ttcgtagcac
    30601 ccttcaaaca gcctatcccc taccgtttga cgattcctca cttcgctcca cttccattac
    30661 agaagtttct tcactactat gggctcggct gacttctcat gattccttgt tactactatt
    30721 tgaacgctca cgagatagat cttacaaaaa atgctttgat ccacaatgga atcaaagcat
    30781 tttaaagagt tcctcataca taagcgcaga agtcgcagtt cctctgtact tggcttcttc
    30841 tcttttgata aagcgagcca agttgagcaa ctcaggtgct ggatgtttgg gatttaggag
    30901 caattcacga ttgaccaggc ctgagagacg aactgcctgc aattgctcat ttgtagtagg
    30961 cagtttttta gcagtctcta ggagagcagc aactaaatct tcactcaaat catgtcgagc
    31021 atgattgtaa agatctttta taaggctttc taggtttggt tctgccatcc ctaccacctc
    31081 ccttatggtt taataatgtt taatcaaatc aaccgttgaa cgatccaatt tcttcaccaa
    31141 ggcttgtaag aaagcttgcg cttctaggaa gtcatccatt gcatagaggg tttggtgaga
    31201 atggatataa cgagcgcaga caccgatagt tgttgatggg acaccaccat ttttcagatg
    31261 agctgcacct gcatctgttc cgcctttacc acagtagtat tggtacttga taccagcttc
    31321 ttcagccgtt gtcaaaagaa aatccttcat ccctgggaga agcaagtgac ctggatcata
    31381 gaaacgaatc aaggttccat ctccaatctt gccttgacca ccgtagacat cacctgctgg
    31441 tgagcaatca actgcgagga agacttctgg gtcaaacttg gttgtagagg tatgagcgcc
    31501 acgcagacca acttcttctt ggacgttaga acccagatag agttcattgc cgagtttttg
    31561 acctgataaa gcttcagcta gctcgcttac catgaggaca ccgtagcggt tatcccaagc
    31621 ttttgagatg atattttttt cattggctgt caaaattgca gaactatctg gtacgatggt
    31681 atcaccagga cggatgccaa aactttctgc ctcagccttg tccgcaaaac caccatcaaa
    31741 aacgatatcg gcaatggctg gcatggttgg tccccccttt ccacgagtca aatgcggagg
    31801 aacagaacct gaaatcacag gaatttcatg accatcacga gtcaagagtt tgaaacgttg
    31861 gctgctaacc accatggggt tccagccacc gatttctacg acacggaagg taccatctgg
    31921 cttgatttcg ctgaccataa aaccaacttc gtccatatga gaagcgacca agacgcgcgg
    31981 tgcatccaca gcttctgaat gtttgatacc aaaaatacca cccaagccat ctgtcaccac
    32041 ttcatccaca tgcggtgtca acttttcacg aagataagca cggacaggcg cttcatgacc
    32101 tgagactgca gcaagttctg ttacttcttt aatttttgaa aataatgttg tcatttcagt
    32161 tccttctttc tttcatccat tttaccactt tttataggag aaggatagtg ggaaggtgga
    32221 tttctaagtt agtatcttag tcctgctcta tcttagaaaa ggatagtatt ctcttgcatg
    32281 tagtgcaaaa tctagtaaac attccaaaat taactcgaat atttatttcc aaacaaaaaa
    32341 acaatacacc atcaaagttg tttgtatttt tcatgaaatt tacagaaaat agttgacttc
    32401 cctttcttct ttctttaaat atatagttgg ttgagtttgg aatagtacgc tgtagctgct
    32461 gaaacatttc tagaaattaa tttgactttc ctaatagagt tgttcatatc ttatttcaat
    32521 ttactatagt acaaaactag aaaaggaaaa aatcatgacc aaaacaattc ttgttacagg
    32581 tgggactagc tacattggct cccataccgt taaagctctt ttaaatgctg gctataaggt
    32641 gcatgtcctc gataatctct ccacaggaaa tcgagcggct gtggatagtc gtgctagctt
    32701 taaagaactg gatgtttatg atgctagcgc cttaaaggct tacttagaag aaaatcagat
    32761 tgatgctgtc ctccattgtg caggtgaaat tgttgtgagc gaaagtattg aaaatccaag
    32821 taaatacttc actgccaacg ttgctggtat gaaccaagtt ctcaaagtct tatctgaagt
    32881 tggcattcaa aaaatcatgt tctcttcgac tgcttctctc tatggaaata actgtattga
    32941 caagccagtg actgaagata ccctgctcga ccctgtcaac ccttatgcag agacaaaact
    33001 gatgggcgaa cgaatgattt actggatggc caatcgctac gactggaaat atgttatttt
    33061 ccgttacttt aatgttgctg gggctgaaat ggatgcttca aacggtctgc gtgtgaaaaa
    33121 tccaactcac atcattccaa atatcaacaa gaccgcattg ggacaaaatg atagcttaaa
    33181 aatatttgga gatgactacg atacacgtga cggttcatgt attcgagatt acatttatgt
    33241 cttggacctt gcataggctc atgttaaagg aatgaactac ctatttcaag aagacagttc
    33301 ttctcaaatc tttaacttag gaactaaaaa aggctatact atcaaagaaa tctttaaaac
    33361 tgctgaagaa ttactaaatt aaaaaatccc acacgagatt gttgctcgac gtgctagtga
    33421 cccagccagt gtcctagcag atgcttcaaa agctaaacaa tatcttgatt ggacggctag
    33481 ctactccctc gaagatatta ttttatcaga ttaaggtaaa aacattttag aataggataa
    33541 caaaggagaa agtcttgttg ctctctcctt gttgtattta ttaaattgtc agaaatttta
    33601 ttgacagatt agaaaaataa tgttacattt atatccgcag atatctttcg ataccaaatc
    33661 tacatgaaag gacggggtat gaaactttct cattatttaa ttggcttact tctactccta
    33721 gtctttctct ctattagcat tgggaccagt gatttttcat ggggaaagct atttgatttc
    33781 gaccagcaga catggctcct ctttcaagag tcccgtctcc caagaactat cagtattctc
    33841 ctgactgcct ctagtatgag tatggcaggc cttctcatgc agactattac ccaaaatcag
    33901 tttgctgcac cgagtacagt tggaacgact gaagccgcca aactgggaat ggtgctgagc
    33961 ctttttgtct ttccatcggc tagtctgacc caaaagatgc tcttcgcttt tgtttcatcc
    34021 atcgtattca ccctcttctt cctagccttt atgaccattt ttactgtaaa ggaaaggtgg
    34081 atgttgcctc tgattgggat catctatagc gggattatcg gctcagtcac agaagttatc
    34141 gcctatcgtt tcaatctggt tcagagtatg actgcctgga cccagggctc cttctccatg
    34201 attcagaccc atcagtatga gtggctcttc ttaggcctca tcatcctgat aaccgtttgg
    34261 aaattatccc aaaccttcac catcatgaat ctggggaaag aaatcagcga gagtttgggg
    34321 atttcctact ccctacttga aaaactggcc ctctttctgg tggcgctaac gacaagcgtc
    34381 accatgatta ccgtgggtgg cctaccattt ctcggagtta tcgttcccaa tcttgttcgc
    34441 aagcgctatg gagataatct aagtcaaacc aaactcatgg tcgcactggt tggtgccaat
    34501 ctagttctgg cttgcgatat cctatcccga gttctgatta ggccctatga gttgtctgtc
    34561 agtctcttgc taggaatcat cggtagtctc ggctttatcc tacttctctg gagaggggga
    34621 cgaaaagatg cagactaaaa gcaaacatac caagctcttc tggattctca ttattcttgc
    34681 catcggagct tgtcttctct acttttggcc catcactcac ttgtcagcct ttgcttggaa
    34741 gttgcgttcc caaaagatca tcgtttatct cttggtagcc atcgcgactg ggatttcgac
    34801 cattagtttt caaaccctga cggaaaatcg cttcctgacg cctagtattt tgggaattga
    34861 atccttctac gtcctactac aaaccctact actggttttt gaaagcaagt ttcttcaact
    34921 tggcaaatcc cctatcttag aattcctagt cttacttctt gtccagtccc tcttctttct
    34981 caccttacaa ggttacttga agacactgat gaagcaagac ctggtcttca tcctgctgat
    35041 ctgtctagcg ctcagaagtc tctttcgaaa tatcagcacc ttccttcaag tcctaatgga
    35101 tccaaacgaa tacgataaac tgcaaaatag tctttttgcc tcctttcaac atctcaacac
    35161 ttccatccta gccatcggtt ctctgatcat cctcgctttg acaatctttt tctttcgaaa
    35221 agcagtcgtt ctagatgtct tgcacctgca aagagaaacg gctcagatat tgggactcga
    35281 tgttgaaaaa gaacagaaag agctcctctg gggaatcgtg cttttgacct caacggccac
    35341 tgccttggta ggacctatgg ccttcttcgg ctttatgctg gccaacctca cctacctgat
    35401 tgtcaaagac tatcagcaca agttactctt tatagtggcc attctggttg gatttattag
    35461 cttaaccttg gggcaagcct tgattgaacg agtctttgca ctggaaattc gtatcagtat
    35521 gatcattgag agtgtgggtg gcttcttatt ctttatctta ctatatagga ggtctcgtca
    35581 gtgaaactgg aaaacattga caaatccatt caaaaacagg atattttgca aggcatttcg
    35641 cttgaagtca gtcctcaaaa actgacagcc tttattggtc caaatggtgc tggaaaatcg
    35701 actctcctct ccatcatgag cagactaacc aagaaagatc agggagttct cagtatcaaa
    35761 ggacgtgaaa tcgagagctg gaattcgcaa gaactggccc aagaactaac catcctaaaa
    35821 cagaaaatca attaccaagc caaattgact gttgaagaac tggtcagttt tggacgtttt
    35881 ccctacagcc gaggtcgact tagatcagaa gactgggaaa aaatccgaga aactctgaac
    35941 tatttggaac tgaccaactt aaaagaccgc tacatcaata gcctgtcagg ggggcaactc
    36001 cagcgcgtct ttatcgctat ggtactggcc caggatacgg actttatctt gctggacgaa
    36061 ccactcaaca atctcgatat caagcaaagc gtcagcatga tgcagattct tcgacgactg
    36121 gtggaggaac tcggcaagac cattatcatc gtcctccacg atatcaacat ggccagtcag
    36181 tatgcagatg aaattgtcgc cttcaaggac ggccaggtct ttagcaaggg aagcaccgat
    36241 caaatcatgc aggctgacct gctcagtcaa ctttatgaga ttcccatcac gctagctgat
    36301 atcaatgaca aaaagatctg tatctatagc tagtaacata aaagctcaag ttagagaacc
    36361 ttcagtctct tagtcaataa gattaagaga ctccctaaat cgttatcaca ttttaaaaag
    36421 gagaaattat gaaaacatcc cttaaacttt atttcactgc cctagtggcc agcttcttgc
    36481 tcctacttgg tgcatgtagt acaaactcaa gcactagtca gacagagacc agtagctctg
    36541 ctccaacaga ggtaaccatt aaaagttcac tggacgaggt caaactttcc aaagttcctg
    36601 aaaagattgt gacctttgac ctcggcgctg cggatactat tcgcgcttta ggatttgaaa
    36661 aaaatatcgt cggaatgcct acaaaaactg ttccgactta tctaaaagac ctagtgggaa
    36721 ctgtcaaaaa tgttggttct atgaaagaac ctgatttaga agctatcgcc gcccttgagc
    36781 ctgatttgat tatcgcttca ccacgtacac aaaaattcgt agacaaattc aaagaaatcg
    36841 ccccaaccgt tctcttccaa gcaagcaagg acgactactg gacttctacc aaggctaata
    36901 tcgaatcctt agcaagtgcc ttcggcgaaa ctggtacaca gaaagccaag gaagaattgg
    36961 ccaagctaga caagagcatc caagaagtcg ctactaaaaa tgaaagctct gacaaaaaag
    37021 cccttgcgat cctccttaat gaaggaaaaa tggcagcctt tggtgccaaa tctcgtttct
    37081 ctttcttgta ccaaaccttg aaattcaaac caactgatac aaaatttgaa gactcacgcc
    37141 acggacaaga agtcagcttt gaaagtgtca aagaaatcaa ccctgccatc ctctttgtca
    37201 tcaaccgtac ccttgccatc ggtggggaca actctagcaa cgacggtgtc ctagaaaatg
    37261 cccttatcgc tgaaacacct gctgctaaaa atggtaagat tatccaacta acaccagacc
    37321 tctggtatct aagcggaggc ggacttgaat caacaaaact catgattgaa gacatacaaa
    37381 aagctttgaa ataagccact tcaaactcaa tttaatactc aatgaaaatc aaagagcaaa
    37441 ctaggaaact agccgcaggt tgctcaaaac actgttttga ggttgcagat gaaagctgac
    37501 gcggtttgaa gggattttcg aagagtataa aattttgaca ttctaaaacg catcctatca
    37561 agttcactca aacctgatag gatgcgtttt tatcattttg aaatctattt accccacctc
    37621 attccccttc ttgatttcgt ttaagggaaa agtggtctgg aacggggtcc ttacctgttt
    37681 tcgaccaggg atgacaacgt aaaatccgag ccaagcccat caataccccc ttaaaaccat
    37741 gtttttcaat agcctgaatc atgtagttgg aacaagtcag ctcaaagcga caagagggtg
    37801 gaaagactgg tgagataaaa cgttggtaaa agcgcacagg cgctattaaa attcgtttca
    37861 ttatttctta gttacagcca tggtgtgtag ttggctgatt tcttttttat taagacgacg
    37921 ggattctcct ggacggagtc ctgtcaagtc taggtgtccg aaacgagtcc gagacaactt
    37981 atctacttgg agaccaacag cttcaaacat ctttttaacc tgatggttac gcccttcatg
    38041 gatggtcaac tgcaccacag agcgattttt gactgggtcc actttgagaa tttcataaac
    38101 agctggcttg gttttcttac catcaatctc aagaccacgg gtcaaggggc ggagattgtc
    38161 cttattggcc acacccttaa cacgcgcgac ataaaccttg tcaatctcat tacgagggtg
    38221 aatcatctcg tctgtaaagt ccccatcatt ggtcaaaatc aagacacctg atgtatccca
    38281 gtccaaacga cccacagggt aaatacgctc tttgacattg ggcaagaggt cgacaaccgt
    38341 cttgcgaccc ttatcatctg tcacactgga aatcacaccg cgtggtttgt taagcagata
    38401 gtagaccttt tcttcgttgt agataggttg accttcaact tcgaccttgt cgcctgactt
    38461 gatagtggtt gctagttcac gcaccacttg gccgttaacc gtcaccaagc cttgcttaat
    38521 cagctcttct gcttttctcc tactggccac acctgcgtgg gcaatatact tattgattct
    38581 catcttcttc tatcctttca ccaaataatt ggctttcttg ggcttgaatc tcaagctcat
    38641 caatcactgg taattcttct aaatggttta tccccatgta atctaggaaa taatccgtag
    38701 tcacatagag gttggggcgc cccaatactt cctttttccc gtcttccttt atcaggtcaa
    38761 aagcctgcaa ctttgccaag gctccactcg agttaactcc acggatggca tcaatttcta
    38821 tccgcgtaat cggctgtttg taggcaataa tggacaaggt ctcaagggca gcccgagaca
    38881 agctctggtt gataggcgcc ttagagtatt ccttcaaaat ctctgcaaat tgagacttgg
    38941 tcaccaatct ataagcacca cttgtctcaa tcaaagccaa actggaatct gggtcctttt
    39001 catacttctg ggctaatttt cctaaactct gctggatgcc tgtcggtggc agagagagga
    39061 gttcagctaa ctggcggacc cgaatcccat cttcacccgc tacaaacaag agcgcttcta
    39121 tttttgctaa agtactcatc tttcctctct atcaagtcta gctttgaggc acttgacttt
    39181 cttccttctt ttccatgaga tagatatctc caaaactctc ctcttgcacg aggatcagct
    39241 cctgggtttt gattaactct agggttgcca aaaagagggt gatgacctct tggacattct
    39301 gggcttcctt gaacaaatcc tgcaagcgca attgatctcg tccaatcaag gactctttca
    39361 cgataatcat catgtcctca atcttatact catcccgcaa gatagtcgtg tgattttgtg
    39421 caaactcctc ttttttcttg gctaggatat ttgaaaaagc caaaaagagg tcaatggtcg
    39481 tcttgtcatg cacaagctcc gcatcttcgt aaatcaactc tgtcggcgct ttggaataat
    39541 actgggcccg ttcttggtgc ttggcttcca agtgctcacc caagagcttg aacttgcgat
    39601 attcttcgat ttgagagagg aggtcctgct ccaggtcatc ccccaagtct gtcacttctg
    39661 ctaccttcgg aaggagttta cgactcttaa tcagcatgag ctgactagcc atgaccatgt
    39721 actcacccgt cacttccaga cgcatggcct gcagggttga aacataggct agatactgtt
    39781 cgatgacttc cgtaatgggc acatcgtaga tatccatctg gtacttagaa accagatgca
    39841 agagcaagtc cagggggcct tcaaaatctt ttaatttaat atccattatc tatatttttc
    39901 taaggtcagg actgttttta atcctaattt ttttgcaatt tcgtacaaat cgaccttgtt
    39961 ttctatttgt cttagaataa actgttcacg taagacttga gctgataagg atggaaaacc
    40021 tttctccttg acaaaagatt ctaactgacg aaaggcccac tgacgagaat agggtttctc
    40081 tcctctttca aaaagatagg tctgccccat caagggttcc aattctgaaa gcaaggccgt
    40141 gggaatggtg acaatcctct gttgggaagc cttgctgatt cgcaacacct gaaaatccag
    40201 attgatgtcc gcaaccttga tggctaaaat ctcactgggc aagagcccca tttctaggat
    40261 taagagcgct agcaagcggc cctctggatg gtcgctttcc tgccaaaaag agtctaggta
    40321 tagaatctct ggcttttccg tcttcttttc agcttgtttg gctaattcca agcggtaaaa
    40381 gctgtccacc tctccttttt gatagagaaa gtatagaaat tggttacagg ccgaaatctt
    40441 tcgcttctgg gcgctgattt ttagattggc tagctgggct tggtaaatct tgagactggt
    40501 ctcagaaatc cgctcaccta ccatgtctaa aaattgctcc aaatcatact tataggactg
    40561 cttggaattg acagacaagc cctgcttttc ctctaaaaag gctgaaatcc tgtctctcat
    40621 tggcatcgaa tctcatattc cttactaaag tcatgcaaga gggcattaac ggccttgagg
    40681 atggacttac gcgtaataat cccttggaaa ataccctctg catccacaac cggtaagaag
    40741 gactcatcta ctagcttgtg caagacctcc gtaatggtga aatcaggcga aacaaccgct
    40801 acgtccgttt ttgtcatatg aacgatatcc gtatccgcca tgatttcttg gctcaagtca
    40861 tgctccatct gataagccat aatatctctg agtccaatcg tcccaacaaa ctgtttttca
    40921 tctgtcacaa cgggaacacg ggtataggtc atctgactga gcaagagggt cgcatgatcc
    40981 gcattgtggg tatcaatcaa cacagctaga tttttagcag gggtcaaaaa agtttcctcc
    41041 tgccccaaca agaaagtctc aaactccttg gcaatcatcg gctaaactcc ttggacaaac
    41101 ctggatacac ctcgtgatct cgtgtcaaaa agtccacttt gaagtaacta tcatcaatct
    41161 ccacacgagc atagagacat tctctgatgg tacctcgtgg ttgactgata gaacctggat
    41221 ttagaaagag gatcttgcct tccaaccaag cacttggcac atgcaagtga ccatagaggc
    41281 agatagcggc ctcttcctcc tgagcccagt agtccaactt ttgaaagttg aaattgatgt
    41341 caaacaagtg accatgagtt tggataatct tggtcgaacc aagctcagtc accagacgtt
    41401 ctgggtagcc ggcgtagaag tccatgtttc ctttaacaac gcggatgccc tcccaaagtg
    41461 gagaatccgg acgtagttca gaatcgccgt tatgaaaaac ggcatcgact ttgcccacat
    41521 agcgatcacg gacttcttcc acaatcaagc tatcgccatg ggaatcgctc attacaatga
    41581 tggtttgctt tgccatgatg gaaatacctc caaaagtttc ttaacggcta aggcacggtg
    41641 agattgacta tttttttctt ccagggttaa ttcagctgat gacttacctg tctctcctac
    41701 aaggaagagg ggatcatagc caaagccatt ttcaccctta ggttcaaagt taatataacc
    41761 tgaccagtct gcttcaacaa ctaaactttc cttatttggg ctggctacga ctagggttgt
    41821 gtggaactga gccgagcggt ccttgagttc aaagaccatg gccaattcgt gcaagagttt
    41881 ggcattattt tcacggtcag ttgctcccac acctgcgaaa cgagctgacc agacgcctgg
    41941 taagccacca aggacatcga ctttgagacc agaatcatct gccaaaacca tcttgcccgt
    42001 taattgagaa atggtttctg ccttaaggcg ggcattttct tcaaaggtca tacctgtttc
    42061 tgctacttca ggcaggtcag ggtagtcatt aagattttcc acatcgtagc ctaacttatc
    42121 aaagatagct cggaattcct tggttttgcc ttcgttacga gtcgcaatca acaaggtttc
    42181 tctgaccttg cttgtctcaa agaaatcatg aacattgacc ccttctttag gcaattctac
    42241 atacaagagt tgcccatttt caaccaagag cagggcccca tgacgttgaa taacttccaa
    42301 gtttcgtccc atttcttgat taagcatgtc taccacaaag gagaacaaac ggtaggtaga
    42361 accatagcgt aaaacagtaa ctgaaagcgg gaaaccatac tcttcatctc caaatatttt
    42421 ggagaattca aacagaggaa aatctgtctt atagtcgctc aaactgttaa cgccaccaaa
    42481 aatactataa gacccaacat accaatcctg gtcatcctta tattcataaa ttttatttgt
    42541 cataattcta catgctccac ataaatctct ttttccagcc attcttcacc aatttgtgca
    42601 aaactttggc tactggctgt tgtgtaaaaa cggtgatgga gtggtccagc atcgcgacca
    42661 cgattgattt caaaataatt gagtaagact gagatatccc gtacgcactc tgccccacta
    42721 tcgatgagct gaacctttgg ccccatgaca ttttggataa tagggcgaag gagtggataa
    42781 tgagtacagc ccaaaatcag gctatccacc tttccaacca agggacgcag ggtttcatag
    42841 accaccttct tggtaacact ggttgacagg gcacctgact caaccaaggg agcaaacttg
    42901 ggacaggcca agctctccac ctgtaagtcg ggatccagat catggatttt ctgacggtat
    42961 atgtctgatt gtaccgtcat gggcgttcca atcactccga ttttcccacc ttgactggac
    43021 ttgatggctg ccgaagctcc tggcaaaatt acacccaaga caggaatatc tagttgagcc
    43081 ttgatttctt cccagacgac cgcagtcgca gtgttacaag caatgacaat cattttgaca
    43141 tccttggtca agagaaagtt gaccagctgc caagtatatt cacgaatttg ctcagctgga
    43201 cgggggccat agggcgcccg cgccgaatct ccaatataga cgatttcttc atggggaagc
    43261 tggcgcatga gctcgcgcac aacggtcaag cccccgacac ccgaatccaa aaaaccaatt
    43321 ggtcgattat ccatacagtc ttctttctag tcttttttct gattgatcgt tcattatgat
    43381 tccttgtact gaacgaactg accgacagct tgatagaatg tcaactacct ctctcttaat
    43441 cataatcaaa tatcaataga atgcaaggaa aaagtctcat ccctaaacca taaccaacat
    43501 agtgagaagt ctggaacttt catcccagac ttttccttat ttattttttc tttttattgt
    43561 tagcaagggc tgccttttgt tggcggatga tttggtggta aacttgttgt accttagctt
    43621 cgcttggttt ttgaccattt gcactcaaaa gagtacgaac tgcttcagca ttcaaacgtg
    43681 ggttgtcagc aaattctttt tcgatttgct tacgaaccaa gtacattcct ccaagagctc
    43741 ctcctagaaa agctagcaca atcaatacaa ttgctaaaag taaatccatc atttttctcc
    43801 tgtttctttt tgagtccctt tcattatacc acaaactagc caccctgacc agtttgttct
    43861 acactttcag ggatggaata gacagcaaaa agaaccctgc ttttctgaaa aagaggtctc
    43921 cttgctaagt ttaatttaca taaatagcca gtgtttgata gggttttagt agaattttct
    43981 cagacacttc tgcatcttca tagtttgaaa tcaaaatctg tccattttgg tagactgctg
    44041 gcaagtcgat ttccacttct ttagcataaa agttattgag cactagtaac ttttgatcct
    44101 caaactggcg ttcaaaagcg tagacttgtt tgctatcttc aaaggctggt ttgtaacttc
    44161 cttctgaaat gattggcatt tccttacgca gtcgaatcaa gtcttgatag aaggtaaaaa
    44221 tcggaccctg gatttcattt tctacattga tgtctttata ggatttacca gctttcaacc
    44281 aaggagtgcc tgttgaaaat cctgcatttt ccgaagcatc ccactgcatg ggaatgcgtg
    44341 aattatcacg cgacttagct tgaataatct ggaaggcttc ttgctgactc tttccttctt
    44401 ctaggagcat ctgataggca ttaagcgatt cgacatccac gtaatcagcc atagaatcat
    44461 agtctgggtc aatcatcccg atttcctcac ccatgtagat ataaggtgtc ccacgtgaca
    44521 ggtgaatgct ggctgctagc atggtggctc cttccttgcg gaagttttga atatcgacaa
    44581 aacggttcaa ggcacgtggt tgatcgtgat tgttccaaaa gagggcactc caaccgtctt
    44641 tatcactcat ttccttaccc caactatggt aaagactctt caactcttca aaatcaaagg
    44701 gagccaaggt ccacttttgt ccatccttat agtccacctt gaggtgatga aaattaaagg
    44761 tcatggataa ttcctgacga tcaggcgacg aatagaggac acagttttcc atggtggtag
    44821 aagacatttc cccaactgtc ataaagctat cgtcagatcc aaaagtggct tggttcatca
    44881 tacgcaaata gttatgaacg atgggtttgt ctgtataagc tggcttccct tcattttcag
    44941 gacagtccac tgaaacctcg tccttaccaa tcaaattgat cacatcaaat cgaaaacctt
    45001 tgacaccctt gtcgcgccag aaattaacaa ccttgaaaag ctccttacgg acattgggat
    45061 tgcgccagtt aaggtcagcc tgggtctcat caaataggtg aagatagtat ttcccagtat
    45121 ccccgaaagg cgcccatgca gaaccaccaa acttagactg ccaatctgtt ggttggtctt
    45181 ggatgaagaa aaagtcttga taatacttat caccagctag ggctttctga aaccattcat
    45241 gctctgtcga acaatgatta agtaccatgt ccagcataaa gtcaatcttg tgctctttac
    45301 cgatacacac catttcctca aaatcagcca tatcaccaaa aagaggatcc actgccatat
    45361 aatctgaaat atcgtaacca ttatcccgtt gaggacttgg atagaatgga ttgagccaga
    45421 ccatatccac acctagtttg gctagatagg gaatttttcc gataatccca cggaaatccc
    45481 caataccatt gccagtggtg tctttgtaag attttggata gatttgatag actacttttc
    45541 ctttatcaag tgtcatctgt ttctcctttt ctgataaaag ggaggaagca gtcttccgtc
    45601 cctatttgtg ctatttcaat tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    45661 cacaggttgc tcaaaacact gttttgaggt tgcagataga gctgacgtgg tttgaagaga
    45721 ttttcgaaga gtattagatt cgtgtagcga ccatgagaga tgctccagct tgaatcgttg
    45781 tcggataagt tccgggaata gtcgctgtat aagcatcttg gttggtgatg ataacaggag
    45841 tttctgtcac cagacctgca gccttaatga catccatatc aaaacgaatc agttgctgac
    45901 caactgtaac gtgatctcct tggactacaa gactttcaaa acctttgcca tcaagaccta
    45961 ctgtatccat accgatgtgg atgagcaatt caactccctc gtcagagaca atgccgatgg
    46021 catgcttggt agggaaaaga accgtcactg tcccattaac tggagaggtc aactcacctt
    46081 ggcttggttc aatgactaga ccttgcccca tgacacctga tgcaaaaata ggatccgtcg
    46141 cttgactcaa ttctttcact tggccagtta gtgggctgat aatttctacc gaagtaagtt
    46201 ctactggttc atggttcaca aattctgctt cttcttgagc aacgaattct gcctgcaagt
    46261 tcgtatcgcc ctctgttttt gtaaagagac cagccttgcg gaagaagaaa gtcaagagca
    46321 ttggaacaac aatcgcaact agcatagttc ctgcaaatgg cagcatgtat tgaggttgaa
    46381 tagagagaat acctggcaaa ccaccgatac caatagaagc cgcagttaca ttaaaagtaa
    46441 cggataacat gcctgcaagg gctgaaccag tcatcccagc aacaaatgga taaatatatt
    46501 ttacgttaac cccaaaaaga gctggttctg taacaccgag ataggctgaa atggttgcag
    46561 gaagtgaaac ctgagcctca cgctcatcat ggcgatgcat gaaataatag gcaaacacgg
    46621 ctgagccttg agcaatatta gaaagagcaa tcattggcca tagggcagtg ccaccagcat
    46681 ccgcaatcaa ttgtgtatca atggcattgg tcatatggtg cagacctgtg atgacaaatg
    46741 gagcgtagag ggcaccaaaa attgcaccga agagccattt aactggacca gttaaacctg
    46801 ccaagacaac tgatgaaagt ccttgtccaa ttgtccaacc gattggtccc aaaacagtat
    46861 gagccaaaat caaggctgga atcaatgaca agaaaggtac aaaaatcata gaaatgactt
    46921 ctgggatatg cttgcgccag aagatttcaa gataagatag actcaaacct gcaagcaagg
    46981 ctgggataac ttgggcttgg taaccgatac gattaacagt aaaatagcca aaattccaaa
    47041 cccagtttgc cgcgatatca gctgctggcg ttgaagcaac cgcataggca ttgagcaact
    47101 gaggcgatac caaacagatt ccgagaacaa ttcccaaaat ttggctggtt cccatcttac
    47161 gagaaacaga ccaagtaatc cctactggta agaactggaa gatagcttca ccaggcaacc
    47221 agaggaaatg attaacacct gcccaaaact gagaggattc tgtgatggtc ttgccatcca
    47281 acatcgacca atggacacct tccaagacat tacggaaacc gaggatcaat cctccgacta
    47341 tcaaggctgg aataatcgga gtaaaaatct ccgccagagt ggtcatgaca cgttggacca
    47401 cgttttgatt actcttagct gcagacttgg ctgcttcttt ggaaacaccc tcaatacctg
    47461 aaacagctgt aaaatcatta taaaagatgg gcacgtcatt tccaatgatt acctgaaatt
    47521 gacctgcatt tgtaaaggtt cctttaacag ctggaattga ctcgatagct ttaacattag
    47581 ccttcttatc atttcctaaa acaaagcgca tccgtgttgc acagtgagtt acggcagtca
    47641 cattttcttt gcctccgatt gcctgaagca gatctttggc ttcttgttca aattttccca
    47701 tttgattctc cttatacttg tcaaagcaag tttgatttga taagtatatt gtaaacgata
    47761 ccaaacttgt ctgcaacttg tttggtataa aaagggtgtt tttttgtaaa ttttctagca
    47821 cttgtatggt aaaatagata caggtgttca ttaaactaga ctaaaaacct atttaagcag
    47881 gcaaaatgaa gaaataccaa caattattta agcaaatcca agaaaccatt caaaacgaga
    47941 cttacgctgt cggagatttc cttcctagcg agcacgacct tatggagcaa tatcaagtga
    48001 gtcgtgatac cgtccgaaag gccctgtctc tcctccaaga ggaaggattg atcaaaaaga
    48061 taagagggca aggttctcaa gtcgtcaaag aagaaaccgt caatttccct gtatccaacc
    48121 taaccagcta ccaagaacta gttaaagaac ttggactgcg ctctaaaacc aacgtggtca
    48181 gtctggacaa gattattatt gataaaaaat cctcactgat aaccggtttc ccagagtttc
    48241 ggatggtttg gaaggtggtc cgccagcgtg tggtggatga tctggtatcc gttctggata
    48301 cggactatct ggatatggaa ctcatcccaa atctcactcg ccaaattgct gagcagtcta
    48361 tctattctta tatagaaaat ggcctcaaac tccttattga ttatgctcag aaggaaatca
    48421 ccattgacca ctcaagcgac cgagacaaga ttctcatgga cattggcaaa gacccttatg
    48481 tcgtttcgat taaatcaaaa gtctatctcc aagacgggcg ccaatttcag tttaccgaaa
    48541 gtcgccataa gttagaaaaa tttagatttg tagattttgc aaaacgcaag aaataaaaga
    48601 ctgagacacc agatctcagc ctttttcttt atcccaaaac taattcatca ctgaccagac
    48661 tttggccact atttttctgg aagagatgca taaggtcttc aactgtcaga tgctttttct
    48721 cttctccctt gacatccacc actatcttgc cttgatagag cataatgaga cgattgccgt
    48781 actcaatcgc atgattcata tcatgggtaa tcatcaaagt cgtccactga tggtactcca
    48841 caatcttttg cgtcaagtcc atcaccattt gactggtttt cgggtcaagt gccgcagtat
    48901 gttcatccaa aagcaaaagt ttgagtttca ccagagctgc catgactagg gtcaaagcct
    48961 gtctttgtcc tcctgagaga tattgagtat ctacttttaa gcggttttca agaccaatat
    49021 tcaactcctt caaggcctct tggaactgga ttctatcctt ctccttcacg ccccaaccaa
    49081 gccctctctt ttgcccacgt ctcaaggcaa tagccatatt ctcctcaatc gtcaaacgag
    49141 aagctgttcc catcttagga tcttgaaaaa cacgggcaat atccttggct ctctttctga
    49201 cactcagatg tttaatggat ttccctgcca gtagaaggtc cccttcgtcc accgatagat
    49261 taccagccaa gatattcatc aaagtggatt ttcctgctcc atttccacca atcacagaga
    49321 taaaatctcc ctcttcaacc tctaaatcta atcctttgag gacatggttc tcattgaccg
    49381 tccctgcttc aaatgtcttg tgaatatttt caagtgttaa caaacttgcc ataactttct
    49441 cctcctattc atttctcaat ttcagaccac gaatctttaa tctcttttgc aatgctggtg
    49501 caaataaaac caaagcgagc aagattgcat taaagagacg taccaagttt tgatctagat
    49561 ttggaatttc atagatattt aaaataatca aacggtaaac aatagcaccg attccgatgg
    49621 ataataagcg gcctccaatg gtcaagtcgt gtatcaagac ttccgagctc aaaccaacaa
    49681 caatggttcc tgtcccagaa gtcacatctg aaaatccatc attttgggca aacaaggaac
    49741 cacatagggc aatcaaaccg gttgaaatca tgtaaccaac aatcttcatg gtgtttacat
    49801 tgactccatt ggcctcactc atcgggatat tgtccccagt cgaacgcaag accaaaccaa
    49861 tctctgtttt catcaaaagg gtcaaaacca aacaagtaag agacagacta aactaagtga
    49921 gaaaacagct ccttcatttg tcagacccaa gctcgccaac tgtttaaaga cggttgcaga
    49981 atctcccaag gaaagattgg gtacactgcc catgatttta atattgattg aatagagccc
    50041 tgtcaaggtc acaatccctg tcaagagagc tgggattttc atcttggtgt ggagcattcc
    50101 tgatataaga cctgctacca tacctgccag caaagcaagt aaagtcgcaa gccaaggatt
    50161 tgcccctgcc tgtatctgtg atacaacgat tgctgccccc ataggaaagg ctccctcagc
    50221 agtcatatcc gcaatatcca aaatacggaa agtcaagtag accccaattg ccataatcga
    50281 ccacaacaaa ccttctgata aactagataa cacaaaattc atcttaatcc tccttattcc
    50341 ttgccttcta acttactaat atcaattcac aatgcatctg ccatttcttg attagtatgc
    50401 aattccagtt tttcaggcaa ctcaactgct atattttctg gttcctcacc ttttaaaaca
    50461 cgaatcagca tgcgagctgt ctgtcttccc aattcttcat aattggttcc gtagttatac
    50521 aagccaccaa cagcaatcat ttctgttgaa ccaccgaata ctggaacctt gtgtttaata
    50581 gaaacctgct taactgtttc catggttgac gaaatgatat tatcagttgg gacaaaaacc
    50641 atatccacct ctgccatcaa actctctgct gccgccttga cgttattact gtccaagatt
    50701 gttttttcaa caacggtaaa gcctttttct ttcagaaatc gcttagcttc atccttttaa
    50761 acaaccgaat tgggctcgct ctgagtatag aggattccaa tcgttttagc ctttagcaac
    50821 actttcttta tcaaattgat ttgggttgaa atggcatctg atgactgatc gcttgtccca
    50881 gttacattgc ccccaggatg ttctcttgac tcaaccaact tggcactgac tgggtctgtt
    50941 acagctgaaa agataactgg tgtcgtttgc gtcgtattgg ccaagctctg agcagaaggc
    51001 cttgcgatgg ctagaacgac atcgctggat tctgctagtt gctgggaaat cgtttttaga
    51061 tttctttgtt ctccctgtgc attttgcaaa tcaatctcaa tattttcccc ctcaacatag
    51121 ccttgtttgg ccagctcatc cacaaatcct tctctagtag cgtctaagga ctggtgggta
    51181 ataaactgcg aaataccgat acgaaagaca tctcttttct tatctgcctt caactgatgc
    51241 aaactgatca gagaagtcaa gaggatcccc actaccaaga gggggctagt gattttcgaa
    51301 caactttcat aatgaaactc cttctcagaa aagataaaac aaaaaaacgc ctagatacta
    51361 tctaaacggg tacttgaaat aacctaacaa gttacaactt agctggtcca tttagatatt
    51421 catctatatg gaccacaatc aaaaatctta gccacataga tacaaacaaa ttgcttgaac
    51481 tgtttgcatc catggtgaca tgtctacaaa cactatctaa agtagctaaa gtaaaagttt
    51541 tgattcatag ttacggcttg tctcttattc atttcttttt ctgctttctt ttttgatcct
    51601 tcctatccta ccaccttttt catcacctgt caataatatt tcaaaatttt ttaaaatttt
    51661 tcgaaaattc tttcag
//
LOCUS       DAVBCV010000007        46818 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBCV010000007 DAVBCV010000000
VERSION     DAVBCV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.007"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..781
                     /locus_tag="VQ516_000416"
     CDS             <1..781
                     /locus_tag="VQ516_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2510917.1"
                     /translation="TYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYGYRRIYLELRN
                     RGYLVNHKRVQGLMKVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCY
                     TDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSISPNLEQVQTMLEQAFTEKHYENT
                     ILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKS
                     FQSLNQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(910..1113)
                     /locus_tag="VQ516_000417"
     CDS             complement(910..1113)
                     /locus_tag="VQ516_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2510918.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(1106..2188)
                     /locus_tag="VQ516_000418"
     CDS             complement(1106..2188)
                     /locus_tag="VQ516_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2510919.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(2314..3096)
                     /locus_tag="VQ516_000419"
     CDS             complement(2314..3096)
                     /locus_tag="VQ516_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2510920.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3096..4439)
                     /locus_tag="VQ516_000420"
     CDS             complement(3096..4439)
                     /locus_tag="VQ516_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2510921.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4538..5854)
                     /locus_tag="VQ516_000421"
     CDS             complement(4538..5854)
                     /locus_tag="VQ516_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2510922.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            6047..7268
                     /locus_tag="VQ516_000422"
                     /pseudo
     CDS             6047..7268
                     /locus_tag="VQ516_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
     gene            7467..9041
                     /locus_tag="VQ516_000423"
     CDS             7467..9041
                     /locus_tag="VQ516_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2510923.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            9305..10093
                     /gene="codY"
                     /locus_tag="VQ516_000424"
     CDS             9305..10093
                     /gene="codY"
                     /locus_tag="VQ516_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2510924.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            10093..10668
                     /locus_tag="VQ516_000425"
     CDS             10093..10668
                     /locus_tag="VQ516_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2510925.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10750..10835
                     /locus_tag="VQ516_000426"
     tRNA            10750..10835
                     /locus_tag="VQ516_000426"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10784..10786,aa:Leu,seq:aag)
     gene            11118..12248
                     /gene="ugpC"
                     /locus_tag="VQ516_000427"
     CDS             11118..12248
                     /gene="ugpC"
                     /locus_tag="VQ516_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2510926.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            12749..13507
                     /locus_tag="VQ516_000428"
     CDS             12749..13507
                     /locus_tag="VQ516_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2510927.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGLSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            13594..14106
                     /locus_tag="VQ516_000429"
     CDS             13594..14106
                     /locus_tag="VQ516_000429"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2510928.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            14288..15232
                     /locus_tag="VQ516_000430"
     CDS             14288..15232
                     /locus_tag="VQ516_000430"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2510929.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            15241..15918
                     /locus_tag="VQ516_000431"
     CDS             15241..15918
                     /locus_tag="VQ516_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2510930.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            16016..16774
                     /locus_tag="VQ516_000432"
     CDS             16016..16774
                     /locus_tag="VQ516_000432"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2510931.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16812..18317
                     /gene="lytC"
                     /locus_tag="VQ516_000433"
     CDS             16812..18317
                     /gene="lytC"
                     /locus_tag="VQ516_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2510932.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18813..19331
                     /locus_tag="VQ516_000434"
     CDS             18813..19331
                     /locus_tag="VQ516_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2510933.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            19461..19967
                     /locus_tag="VQ516_000435"
     CDS             19461..19967
                     /locus_tag="VQ516_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2510934.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            20169..21401
                     /gene="clpX"
                     /locus_tag="VQ516_000436"
     CDS             20169..21401
                     /gene="clpX"
                     /locus_tag="VQ516_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2510935.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            21410..21997
                     /locus_tag="VQ516_000437"
     CDS             21410..21997
                     /locus_tag="VQ516_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2510936.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            22008..22388
                     /locus_tag="VQ516_000438"
     CDS             22008..22388
                     /locus_tag="VQ516_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2510937.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            22440..23330
                     /gene="rapZ"
                     /locus_tag="VQ516_000439"
     CDS             22440..23330
                     /gene="rapZ"
                     /locus_tag="VQ516_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2510938.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            23327..24304
                     /locus_tag="VQ516_000440"
     CDS             23327..24304
                     /locus_tag="VQ516_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2510939.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            24301..25212
                     /gene="whiA"
                     /locus_tag="VQ516_000441"
     CDS             24301..25212
                     /gene="whiA"
                     /locus_tag="VQ516_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2510940.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(25246..26214)
                     /locus_tag="VQ516_000442"
     CDS             complement(25246..26214)
                     /locus_tag="VQ516_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2510941.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            26350..27207
                     /locus_tag="VQ516_000443"
     CDS             26350..27207
                     /locus_tag="VQ516_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2510942.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            27194..27973
                     /locus_tag="VQ516_000444"
     CDS             27194..27973
                     /locus_tag="VQ516_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2510943.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27997..29349
                     /locus_tag="VQ516_000445"
     CDS             27997..29349
                     /locus_tag="VQ516_000445"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2510944.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAIQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            29423..29797
                     /locus_tag="VQ516_000446"
     CDS             29423..29797
                     /locus_tag="VQ516_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2510945.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29799..30647
                     /locus_tag="VQ516_000447"
     CDS             29799..30647
                     /locus_tag="VQ516_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2510946.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30924..31691
                     /locus_tag="VQ516_000448"
     CDS             30924..31691
                     /locus_tag="VQ516_000448"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2510947.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDDMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31703..32887
                     /locus_tag="VQ516_000449"
     CDS             31703..32887
                     /locus_tag="VQ516_000449"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2510948.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32884..34755
                     /locus_tag="VQ516_000450"
     CDS             32884..34755
                     /locus_tag="VQ516_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2510949.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDSKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34898..36082)
                     /gene="mntE"
                     /locus_tag="VQ516_000451"
     CDS             complement(34898..36082)
                     /gene="mntE"
                     /locus_tag="VQ516_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2510950.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPISEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            36386..39082
                     /locus_tag="VQ516_000452"
     CDS             36386..39082
                     /locus_tag="VQ516_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2510951.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            39095..39886
                     /gene="yghU"
                     /locus_tag="VQ516_000453"
     CDS             39095..39886
                     /gene="yghU"
                     /locus_tag="VQ516_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2510952.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            40002..40412
                     /locus_tag="VQ516_000454"
     CDS             40002..40412
                     /locus_tag="VQ516_000454"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2510953.1"
                     /translation="MEKKIVKDILFLSQVSHPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGMQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            40424..42019
                     /locus_tag="VQ516_000455"
     CDS             40424..42019
                     /locus_tag="VQ516_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2510954.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            42186..42350
                     /locus_tag="VQ516_000456"
     CDS             42186..42350
                     /locus_tag="VQ516_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2510955.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            42429..43157
                     /gene="yaaA"
                     /locus_tag="VQ516_000457"
     CDS             42429..43157
                     /gene="yaaA"
                     /locus_tag="VQ516_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2510956.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(43208..44692)
                     /locus_tag="VQ516_000458"
     CDS             complement(43208..44692)
                     /locus_tag="VQ516_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2510957.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEGYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            44908..>46818
                     /locus_tag="VQ516_000459"
     CDS             44908..>46818
                     /locus_tag="VQ516_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2510958.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDR
                     GYSGFRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVRN
                     RVDFSIRYSGPGEFVNNNFILDKGNGFGEPSRATVSTPKLRDQVKNISKGGNFVSHSG
                     YSLEAAVETSTNQTIKFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDRGERGLTGAQGAKGEKGDRGERGLTG
                     AQGAKGEKGDRGE"
BASE COUNT        13992 a         8661 c        10567 g        13598 t
ORIGIN      
        1 gacctactac tatcacttga aacagctaga caaaacagat aaggaccaag agcttaaagc
       61 tgaaattcaa tccatcttta tcgaacacaa gggaaattat ggttatcgtc gaatttattt
      121 agaactaaga aatcgtggtt atctggtaaa tcataaaaga gttcaaggct tgatgaaagt
      181 actcaattta caagctaaaa tgcgacagaa acgaaaatat tcttctcata aaggagacgt
      241 tggtaagaaa gcagagaatc tcattcaagg ccaatttgaa ggctctaaaa caatggaaaa
      301 gtgctacaca gatgtgacag aatttgccat tccagcaagt actcaaaagc tttacttatc
      361 accagtttta gatggcttta acagtgaaat tatcgcctat aatctttcaa tttcacccaa
      421 cttagaacaa gtacaaacaa tgttggaaca agccttcaca gagaagcact acgagaatac
      481 gattctccat agtgaccaag gctggcaata tcaacacgat tcttatcatc agtttcttga
      541 gggtaaggga attcaagcat ctatgtcacg caagggaaac agccaagaca acggtatgat
      601 ggaatctttc tttgggattc tgaaatcgga aatgttttac ggttatgaga agtcgtttca
      661 gtcgcttaat caattggaac aagctattgt agactatatt gattactaca acaataaacg
      721 aattaaggta aaactaaaag gacttagtcc tgtgcaatac agaactaaat ccttcggata
      781 aattaattgt ctaactgttt gggggcagta catattcaac acctgatact atgcgtttta
      841 ttgtgggaag attttcctca ttttctagtg aaattgaact ttttctaaca tcttttgata
      901 gatgacttct taatcaatta tcatttccat tgttgtttct tggggtttca tcccttgtct
      961 ttgataaaag cggagagcac cctcgttatc attccagaca cttagggtta gattattgca
     1021 gccaatttct ttagcataat agcagaaaaa tgacaataaa atagccccta gatattttga
     1081 aaggaaagca taggggctat atagtttaat caaaataaag caaatctttg ctggtgcttg
     1141 taaagaggtc gaagccatcc ttggtaacaa caccgcagtc ttcaatacga acaccgactt
     1201 taccagggat atagatacct ggttcaacag agaagcacat gccttcttcg atgaccatgt
     1261 cgtttccttc catgatagat gggaattcat ggacatccat accgatacca tgcccgagac
     1321 ggtggttgaa gtactcacca taaccagctt tttcgatgac ctcacgggca gcgcggtcca
     1381 cttcatgagc agtcacacct ggcttgataa agtcaagagc agcttgttgg gcttcaagag
     1441 tcaagttgta aatatctttc ttgaattggt ctggtttgcc gacagcgact gtacgagtca
     1501 tatctgacgc atagccattg accagaacac ccaggtcaaa gaggagaaga gcatcatttt
     1561 caaccttatt agctgctgga atgccgtgtg gattcgcagc attatcacca gtcaagacca
     1621 tggtatcaaa gctcatttca taaccttcac gtttcatggc aaagtcgatt tgtgcgatga
     1681 tatctgtctc agtcttatca agagaaatat tgtcaaaacc aacatgaaca gccttgtcag
     1741 cataaagacc tgcaaccatc attttttgca cttcatcagc tgatttgatg aggcgcatgc
     1801 gttggatacg aggagtgagg ttgtcaaact cagcagtctc aaaaactgtt ttcaaaccat
     1861 ggtatttggt caagatgaga ttgtcaaact caacagcgac acgtttgaag tcaagttgtg
     1921 gaagagcatg tttgattttt tgccatggat tttcagaatc gacatagccc actactggga
     1981 aggaaacggt gctacttgca cgttctactt caagagctgg gacaaagagg agaggttcct
     2041 gatccgctag gacaaagagg aacatttggc gttcatgggg atcactgtaa aaaccagtga
     2101 ggtaattgat tgtgacgggg tcagatacga cagcgacgtc tagtttttct gattcaagat
     2161 atgttaggat ttgttgtaat ttagacatat gctaccttct ttctacccct ctattttggc
     2221 aaaaaatgag agaaaatgca agggagaagg gtaaaagaaa agacaaaaaa ctccgacaag
     2281 ataacggagt tctttgatat cattttcctt tgtttaagaa aagtcagcct tgcttttgac
     2341 gtcactgtac tcttcagcga tttcttggct gagaatgtcc tcataggcag ggagttggat
     2401 gtcctgacca tatttcttct tgagggcagt agtaactagg cgataagcca gattggcatt
     2461 acgagagagg ataggcccgt ggaagtagga accaaagaca ttcttataat gaaccccttc
     2521 accgaccttt tcttcgttgt ttccatttcc atagacaacc tgtcccagcg gtttttggtc
     2581 atcagagagg aaggtacgac cttggtgatt ttcaaatcca tagtaggttt catcgaaatc
     2641 ttcattgtga atcttgatgt caccgataaa acggttattg gtctggttga gcgtgtagtg
     2701 tcccatgacc cctagccctt cgatacgttt tcctgaagct tcaacataat attgacccaa
     2761 tagttggaaa ccaccgcaga tagccagaac tacaccgtcg ttttggatgt agttgtcaat
     2821 gctctctttt ttagcaggta ggtcgtctgc aatgatactt tgttcaaagt cttgaccacc
     2881 accgaaaaag gcgatgtcgt agtgattttc atcaaagtca tcatggagag aaacgatgtc
     2941 aacggtcaca tgggctccca gtttttcagc cacatacttg agcatgagga tgtttccatt
     3001 gtccccgtag gtattcatga gatttccgta gaggtgggca atgttgagct gataggggta
     3061 attgctatct tttgaggaaa gtgaagtata aaccattagt tcatctcctt tctaacaatc
     3121 tgacgactag ccagcagttc acgaaattcc agcatggcag tataagttgc cagaatatag
     3181 gcatgcttgc agtcttgatt ctcaatggtc ttgagaactt gctccagatt actcgtttca
     3241 gtgattttct cagctggata gccagtcact cggaggcgac gagcgatttc agaatgacga
     3301 acaccgccag cgttgatttc aggaatgtcc atgtcagtga tttgctcaaa gtctgcatcc
     3361 cagatccagc tagtgtcaat tccatctgca tagttggcat taaggaggac agataggcta
     3421 aatggataag gtgctagttt gatcatttcg atagcttggg ttgcaccgac tggattttta
     3481 atcaagacaa gggtacattc cttgtcaccg atatgaaagg tttcttggcg tccaaagaca
     3541 gcacggctct tgtcaaatcc ctgtttgatg agttgggaat ctgcgccgag gaaacgggcg
     3601 atggccacag cagctagggc gttatagata ttatagagcc cgccgatttg gataccgtat
     3661 tcttggccgt ctatgacaaa gcgagagcga ttgttggtca actcaaccag ttttgtcaaa
     3721 cgatagtcga gatcaggacg tttacatcca caaccttcac agatataggc acccaagttt
     3781 gcataggtat tatgctcata tttgaggatg ccttggcagt caggacagag aatcccttcg
     3841 gtattgtagt gagccagttg ggctggtcct ttttccaagt caaaaccaaa atactctata
     3901 gggtttggaa tagttggctt gtagaaaagt ggactgtctc cgttaaggag aacagtagca
     3961 gttggaactt ttcgaatggc atccaatatc atgttatagg tagtatagat ttcaccgaaa
     4021 cggtccatct ggtcacggaa gatattagta atgacaaaaa gactaggctg gatatagtca
     4081 cagatacgag atagactggc ttcgtcaatt tcgaggacgg caatattttt cccagttttt
     4141 gaagatttgg ctgttaggaa ggttgttgca atccctgtaa tcatgttggc accgcttggg
     4201 ttggttagaa cttgaccata aacctctttt aaaatgccga cagtgagggc agttgtcagg
     4261 gtttttccat ttgttccagt gacaacgaca atctcgtagt tcttagctag gctttgtaaa
     4321 atatctttat caaattgaag ggcgactttc cctgggagcg tacttccacg tccaagacgg
     4381 cttaaaacga agtgggaaga acgcccagca agaaggccca aagtagtttt taagttcatg
     4441 tttctattat atcacaaaag agggaaaaga cagatttggt gtttcgtaga agtaaaaaca
     4501 gcccaaagag ggctgtcagt taagatgaga tttcaactta aatcgcaaac aagtcattca
     4561 agttctcagc caaggttggg tgagtgaaga tttgttttgt gaagtaagtg taaggaatct
     4621 tgttgtccat agcaacagta atgatgttga tgatttcttg agaaccttct gagaagatgc
     4681 ttgcaccaag aatttctttt gtttcagtat taacaacagc tttgaaagct ccgcgaaggt
     4741 ctccatttac gtgaccacga ggcatggctg caacagggat ttctttcact gcgtatggaa
     4801 gtttcaaatc agctgcttgg ctttcagtca aaccaacttg tgaaagtgca ggtgtgatga
     4861 acatagtatt tggcacattg agacggtcct caagtgtgta gctgccatct ccagcaaggt
     4921 agctgtaaac aacacggaag tcatcaagtg aaatgtaagt aaattgaagg ccaccgttga
     4981 catctccaac tgcaaagaca ccaggaacgt ttgtttgaca gtgtttgtct actttaatag
     5041 caccacgttc agttagttca atatctgtat tttcaagttg aagtggttct acatttggtt
     5101 tgcgtccagt tgcgtagaga agggcgtcga aacggtaagt ttcgtcttca gttacgacaa
     5161 gcacttggtc accatcgttt ttgatttcag tagtatggat attttgaagc aattcaatgc
     5221 catcttcttc catgtattgt ttagcaagag ctgcgatgga aggttctgca cgaggtagga
     5281 atgtatccaa ggcatctagg actgtgacct tgcttccaag tttgttgtaa aggccggcaa
     5341 attcaagacc gatatttccg ccaccaagga ttccaagttt ttcaggtaat ttgtccaagc
     5401 tttggatacc tgttgagtca aagatgtttt tgcttgtagc aagtccagga attggcaaga
     5461 cgtttgaaac agcaccagtg ttgatgacga ttgtttcagc agtcagttct ttcttttcat
     5521 caccagcttg gatttcgatg actttatttg aaaggaagtg agcttccgca tcaaagatat
     5581 ctacgcctgt accagcaaca gtcgcatagt ttttaccgtt gaggcgacca gtgatcgtgt
     5641 ttttagtagc aatcacttct tcaaaagaca agtccttttc agcagcaact agcaaggttt
     5701 tagttgggat acaaccaatg ttgatacaag ttccaccgta catagccttg ctgcgttcaa
     5761 cgagggcaac ttttttgcca gctgaagcca atttacctgc tagtgtttta ccagctttac
     5821 caaatccaat aacaattaaa tcgtatgtta acatttatct tcctcctatt cgaatttcat
     5881 tctagcacaa gaaaaaaact tttgcacaat tctgctacgg aaaagacaga aaaagataaa
     5941 aacgctttcc agacaaaaaa ctagtaattt tttgacaagc ttgataaact aaaatcctat
     6001 ctatttttat ataagaaaga ataaatgttc gaaaatagga ggttttatga aatcgaatcg
     6061 ttatattatt gcctttgctg gggtcatttt acacttaatg ctgggttcga cttatgcttg
     6121 gagtgtttat cgtaacccta ttattgaaaa aacgggatgg gatcaggctt ctgttgcctt
     6181 cgcctttagt ctagcaatct tttgtttggg cttatcggct gcatttatgg ggcgtttggt
     6241 agaaaaattt ggtccgaaag tcatggggag tctatctgct tttctatacg caggtggaaa
     6301 tatcttaaca ggatttgcaa tagaccgtca ggaactgtgg ttgttgtatc tagcttatgg
     6361 cattttaggt gggcttggtt tgggagcagg ctatattacc cctgtgtcaa cgattataaa
     6421 atggtttcct gataaacgtg gtctcgcaac aggtttagcg attatggggt ttggttttgc
     6481 ttctttattg actagtccca tagcgcaaca cctcatcgca ggggtaggga ttgtagaaac
     6541 tttttatatt ttaggagcaa gttactttat tatcatgctc ctagcttcac aattcattaa
     6601 gcgtccaaat gagcaagagc ttgcaatttt atcttcttca gggaaagaaa aaacagcctc
     6661 tttgacgcaa ggaatggctg caaatcaggc tctaaaaagc aatcggtttt atatgctttg
     6721 gattattttc tttatcaaca tagcttgtgg tttaggctta atttcagcgg catcgccaat
     6781 ggcacaggag atggctggct tgtctacaag tcatgcagca gtaatggtgg gtgttttggg
     6841 gattttcaat ggatttggtc gcttgctctg ggcgagtttg tctgactata tcggtcgccc
     6901 tctaaccttt agtatattac tgcttgtcaa tcttttctct ctcactttgg ctctttacag
     6961 attccgtttt atttgtagtt gctatgtcta ttttgatgac ttgctatgga gctggttttt
     7021 ctttgattcc agcttatctc agtgatattt ttggaaccaa ggaattggcc gctctgcatg
     7081 gttatatttt aacagcttgg gcaatggctg gtttagcggg acctatttta ttagcagaga
     7141 cttataaaat ggctcattcg tacacacaaa ccttgttcgt ttttctcatt ttatacagta
     7201 tcgccttggc tttgtcttat tatctaggtc gttcaatcaa aaaagaaagt caaaaaccgc
     7261 ttacatgatt ttctgtagaa atggggctat cttttgctct ggtcttgtgt tatactagat
     7321 aggttgcaaa gaaaacagta cttttctttt gtggaaaaga agcaacacga ttttatatct
     7381 agttatatga aaatacgtca taaaaagaaa agtataaact aagccctata gggtggcttc
     7441 gcaccacctt tagaaagaag aataacgtga aatttaatga attaaacttg tctgctgatt
     7501 tgctagcaga aattgaaaaa gctggttttg tagaagctag tcctatccaa gaacaaacta
     7561 ttcctttggc ccttgaaggc aaggatgtta tcggtcaagc tcagactggt acaggaaaaa
     7621 ctgcagcctt tggcttgcct acccttgaaa aaatccgtac agaagaagcg actatccaag
     7681 ctttggtcat cgctccaact cgtgaactag ctgtccaaag tcaagaagaa ctcttccgct
     7741 ttggtcgtag taagggagtc aaagtccgtt cagtatatgg cggatcaagc attgaaaaac
     7801 aaattaaggc tcttaaatct ggtgcccata tcgtggtggg aactccaggt cgcctcttgg
     7861 acttgattaa acgcaaggcc ttgaaattac aagacattga aacccttatc cttgacgaag
     7921 cggatgaaat gcttaacatg ggcttccttg aagacatcga agccattatt tcccgtgtac
     7981 ctgagaaccg tcaaactttg cttttctcag caactatgcc agatgccatc aaacgtatcg
     8041 gtgttcagtt tatgaaagcc cctgaacatg tcaagattgc ggctaaggaa ttgacaacag
     8101 aattggttga ccagtactat atccgtgtta aggaacaaga aaaatttgac accatgactc
     8161 gtctcatgga tgtggcacaa ccagaactcg ctattgtatt tggtcgtacc aaacgccgtg
     8221 tggatgaatt gactcgtggt ttgaaaattc gtggcttccg tgcagaagga attcatggcg
     8281 acctagacca aaacaaacgt cttcgtgtcc ttcgtgactt taaaaatggc aatcttgatg
     8341 ttttggttgc gacagacgtt gcagcgcgtg gtttggatat ttcaggtgtg acccatgtct
     8401 acaactacga tattccacaa gatcctgaga gttatgttca ccgtatcggt cgtacaggtc
     8461 gtgctggtaa gtcaggtcaa tctattactt ttgttgctcc aaacgaaatg ggttaccttc
     8521 aaatcattga aaacttgact aagaaacgca tgaaaggtct caaacctgca agtgtagaag
     8581 aatccttcca atcaaaaaaa caggtagctc tcaagaaaat cgaacgtgat tttgcagatg
     8641 aaaccattcg tgccaacttt gagaaatttg gtaaggatgc tcgcaaattg gctgctgagt
     8701 ttactccaga agaattggca atgtatatct tgagtctgac agtccaagac ccagatagcc
     8761 ttccagaagt ggagattgca cgtgaaaaac cactaccgtt taaaccatca ggtaatggtt
     8821 tcggtggtaa agctaaggga ggtcgtggag gccgtcgtgg ggacgaccgt cgagagcgtg
     8881 atcgccgtgg caatggtcgc cgtgatgagt tcaaaaaagg aagtcgtggc aacgatcgtt
     8941 ttgataagga aaaacgttac cgtaaggata ataaaaaacc acgcaatact ttaagcgaaa
     9001 agcaaacagg ctttgttatt cgtaacaaag gcgataaata agaaaaagcg gttcaaaatg
     9061 aatcgctttt ttatataagg agaccgaatg tgaaaagaca aataaaatta ctatattatc
     9121 ttttataatg ttatgataca caaaaatagg caacttgtca atagaaaagg aacaatttaa
     9181 ttaaaaatat tctggttttt aattaaattg taagtcaatg caagtcaata aggaattttc
     9241 agataattat tgaaaaagag acgtttttat gttataatga tagcgattaa attcgaggtg
     9301 aaaaatggca catttattag aaaaaactag aaaaattaca tcaattttga aacgctcaga
     9361 ggagcagttg caggatgagc tcccttacaa cgctattacg cgtcagttag cggatattat
     9421 tcattgcaat gcctgcatta tcaatagtaa gggacgtctg cttggctatt ttatgcgtta
     9481 taaaacaaat acagatcgcg tagagcaatt cttccaaact aagattttcc cagatgacta
     9541 cgttcaaggg gctaatatga tttacgaaac agaagcaaac ttgcctgttg agcatgatat
     9601 gagtattttc cctgttgaga gtagagatga ttttccagat ggcttgacga ctattgcacc
     9661 gattcatgta tcggggattc gccttggttc tttgattatt tggcgtaatg ataaaaaatt
     9721 cgaagatgag gacttggttc ttgttgagat tgccagtacc gttgttggga ttcagcttct
     9781 taacttccaa cgtgaagaag atgagaaaaa tattcgtcgt cgtactgctg tcaccatggc
     9841 ggtcaatacc ctttcttact ccgaactccg tgctgtttca gcaattttag gggaattaaa
     9901 tggaaatgaa gggaagttga ctgcgtcagt gattgcagat cgtatcggaa tcactcgctc
     9961 tgtgattgtc aatgctcttc gtaaacttga gtctgcgggg attattgaaa gtcgctcact
    10021 tggaatgaag ggaacctatc ttaaggtctt gatttcagat atttttgaag aagtgaagaa
    10081 aagagattac taatgacaaa ggctttaatt tcgattgatt atacagaaga ttttgttgct
    10141 gatagtggga agctgacagc aggtgctcca gctcaggcga tttcggatgc catcagcaag
    10201 gtaactcgat tagcttttga acgaggagat tatatcttct ttactattga tgctcatgaa
    10261 gaaaacgatt ttttccatcc ggaaagtaag ttatttcctc ctcataatct gattgggacg
    10321 agtggacgga atttatatgg agatttgggg atcttttatc aagagcatgg ttcagacagt
    10381 cgtgtctttt ggatggataa acgccattac tcagcttttt cagggactga cctagatatt
    10441 cgtttgagag agcgtagagt gtctaccgtt atcttaacag gtgtcttgac ggatatctgt
    10501 gtcctacata cagctataga tgcctataat ctaggatatg acatcgagat tgttaaacca
    10561 gctgttgctt ccatctggcc tgaaaatcat caatttgccc taggtcattt caaaaataca
    10621 ctcggagcta agttagtaga tgaaaatcta aatgaacttt ctgagtaacc tggttggtga
    10681 aaatgaaaaa atttgaaaaa aagtgttgac aagcatccca aaagttgata tactagtaaa
    10741 gtaatcgatg cggggatggc ggaattggca gacgcgcagg actaaggatc ctgtgaccgc
    10801 tttaggtcgt gagggttcaa gtccctctct ccgcatagga aaagagttag ctaaagctag
    10861 ctcttttgta tttctctaaa aaattagaat ccagtcaaat gtagtggaga atcagcgaaa
    10921 atatgggaaa actgttcctt gtttctgctt ttgtcataga agaataataa agattatctt
    10981 cttcaaattc tccgatattc tctaaagttt tgtgcaagtt gcacagaact tttttatttt
    11041 tttggtcatc ttgccataga aatataaagc gttttcatct ataatataat tatcaaaaga
    11101 caaaaggagt tcacctcatg gtagaattga atcttaaaaa tatttacaaa aaatatccaa
    11161 acagcgaaca ctattcagtt gaagatttca acttgaacat caaagataaa gaatttatcg
    11221 ttttcgtagg accttcagga tgtggtaaat caactacact ccgtatgatt gctggtcttg
    11281 aagacattac agaaggtact gcatctatcg atggcgtggt tgtcaacgac gtagctccaa
    11341 aagaccgtga tatcgccatg gtattccaaa actacgctct ttacccacac atgactgttt
    11401 atgacaacat ggctttcggt ttgaaattgc gtaaatacag caaagaagac attaacaaac
    11461 gtgttcaaga agcagctgaa atacttggat tgaaagaatt cttggaacgt aaaccagctg
    11521 acctttcagg tggtcaacgt caacgtgttg ccatggggcg tgcgattgtc cgtgatgcga
    11581 aagtattctt gatggacgaa cctttgtcaa acttggatgc caaacttcgt gtatcaatgc
    11641 gtgctgaaat cgctaaaatt caccgtcgta tcggagctac aactatctat gtaactcacg
    11701 accaaacaga agcgatgaca cttgcagacc gtatcgttat tatgtcagct actaagaacc
    11761 ctgctggtac aggtactatc ggacgtgtag aacaaatcgg tactcctcaa gaagtttaca
    11821 aaaatccagt taacaaattc gttgcaggat tcatcggaag cccagctatg aacttcatca
    11881 acgtgaaatt ggttggtagc gaaattgttt ctgacggttt ccgtttgaaa gtgccagaag
    11941 gagcattgaa agttcttcgt gaaaaaggct acgaaggaaa agaattgatc tttggtatcc
    12001 gtccagaaga cgtgaatgca gaacctgctt tccttgaaac attcccagac tgtgttgtaa
    12061 aagcgactat ctctgtatca gaactgcttg gttcagaatc tcacctttac tgccaagttg
    12121 gtaaagacga gtttgttgca aaagttgatg ctcgtgacta cttgcaaaca ggtgcaacag
    12181 ttgagcttgg atttgacttg aacaaagcac acttcttcga tgtagaaact gaaaaaacaa
    12241 tctactaaaa taaataaaat tcaaagcact acaagaaaag atatctcttt atcaattgta
    12301 gtggagagat atcagttaat ctagggagag aaacaaaatg cttctctcct ttttgctagg
    12361 gaagtcatat tatgcatcta tattgtgatg ctctttaata ctcttcgaaa atctcttcaa
    12421 accacgtcaa cgtcgccttg ccgtacgtat gattactgat ttcgtcagtt ttatctgcaa
    12481 cctcaaagat gtactttgag cagcttacgg ctagtttcct agtttgctct ttgatttcca
    12541 ttgagtatta tttgtgggta ccatctacaa gtgaagctat atgcgtaaac tacgtgagca
    12601 attgaattcg aactagagag gtaataataa atttatgcta tagttatggt gacttgtatg
    12661 cttttgattc tagtttatca aataatagat tagaattgtc agataatatc attttgtgtt
    12721 ataatgaaga aaaaacagag gtgttcaaat gtcagaagca ggtcataagt ttttagcaaa
    12781 attggggaaa aaacgcttac gtccaggtgg aaagcgtgct acagattggt taattgcaga
    12841 aggaggattg tcaaaagaaa agagaatact agaggttgcg tgtaataggg gaactacagc
    12901 aattgagttg gcacagcgtt ttggttgcaa gataactgct gttgatatgg atgctcaagc
    12961 tttagaagtg gctaaaaaat ctgctggaac ggcaggtgtt gctcatttaa tcagttttga
    13021 aagagcaaat gcaatgaaac ttccttatca agatgctagt tttgatattg ttataaatga
    13081 agctatgctg actatgcaag ccgatcaagc taagaaaaaa tgtgtaatgg aatatctaag
    13141 ggtattaaaa cctggaggtc ttcttttgac acatgatgtg cttcttaagg aagctaaaga
    13201 gtctatcaga caggaattat cacaagcaat tcatgtaaat gtaggtcctt taactcaaga
    13261 tggttgggaa caggtgatga tagaatcagg ttattgtgat gtgaaagtat tgactggtga
    13321 aatgacatta atgaaattat cgggtatgat ttatgacgaa ggttggctag gaactttgaa
    13381 aatttgtgta aatgcttgta aaaaggagaa tagaaagcag tttttaacta tgtataaaat
    13441 gtttgctaag aataaacaga aattgggctt tattgcgatg gctagttata aatcgtcaaa
    13501 acgttagata attattgaag ttaacttttc cttttttctt tcttaaaaaa tatgctataa
    13561 tagagagtga aaaactttga aagaaagaaa aagatgaatt taaaagatta cattgcaaca
    13621 attgaaaatt atccaaagga aggcattacc ttccgtgata ttagtccttt gatggctgat
    13681 ggaaatgctt atagctacgc tgttcgtgaa atcgttcagt atgctactga caagaaagtc
    13741 gacatgatcg tgggacctga agctcgtgga tttatcgtgg gttgtccagt tgcctttgag
    13801 ttgggaattg gttttgcgcc tgttcgtaag ccaggtaaat tgccacgcga agttatttct
    13861 gctgactatg aaaaagagta cggtgtcgat accttgacta tgcacgcgga tgccattaag
    13921 ccaggtcaac gtgttcttat tgtagatgac cttttggcga caggtggaac tgttaaggca
    13981 actatcgaga tgattgaaaa acttggtggt gttatggcag gttgtgcctt ccttgttgaa
    14041 ttggatgaat tgaacggccg tgaaaaaatc ggtgactacg actacaaagt tcttatgcat
    14101 tattaatgaa aacagtccct agggctgttt tctctacact aggatataaa aatagactat
    14161 aactagttag agaaaaacta taattgaaaa ctatatcttc ttgcagtata ataaaaggac
    14221 taagtgtttg agatttgtct tcaaacacat gcaattattc ctgagagagt acagttagga
    14281 gagggttatg ccgattcgaa ttgataaaaa attgccagct gttgagattt tacggacaga
    14341 gaatatcttt gtcatggatg atcaacgtgc tgcccaccaa gatatccgtc ctttgaagat
    14401 tttaatttta aatctcatgc cacagaaaat ggtcacagag acccagttgt tgcgtcactt
    14461 ggctaataca cccctacaac tggatattga ttttctctat atggagagcc accgttctaa
    14521 aacaactcgt tcagagcaca tggagacctt ctataaaact tttcctgaag tcaaggatga
    14581 gtattttgat gggatgatca tcacgggtgc tccagttgag catttaccat ttgaggaagt
    14641 ggactattgg gaggaattta ggcagatgct tgagtggtct aagacacatg tctattcgac
    14701 ccttcatatc tgttgggggg ctcaggctgg gctttatctg cgctatggtg tagaaaaata
    14761 ccagatggac agtaagctat caggtattta tcctcaggac accctaaaag agggtcacct
    14821 tctatttaga ggctttgatg atagctatgt atcccctcat tcacggcaca cggagatttc
    14881 taaggaagag gtcttaaaca agaccaatct cgagatttta tcagaaggac ctcaggttgg
    14941 ggtttctatt ttggccagtc gtgatttacg agaaatttat agttttggtc atttggagta
    15001 tgaccgtgat accttggcaa aagagtattt tcgagatcgt gatgcaggtt tcgacccaca
    15061 tattccagaa aattacttta aagatgatga tgttaatcag gtaccttgtc tttgttggtc
    15121 ttcatctgca gccctctttt tcagtaattg ggtaaaccat gcggtctatc aggagacgcc
    15181 ttttgattgg agaaagatag aagatgatgc atctgcatat gggtatttat aagaggaatt
    15241 atgacatatt tagacgcttt taaatcaggt accttggttt taccgagtgc cctgctcttg
    15301 cattttaagg aactctttcc ttctagcgac gattttctgg tttggcaatt tttctatttg
    15361 caaaatacga caggcttaga agaaatgtcg ccaagccaga ttgctgaaag gattggcaag
    15421 gaaatttcgg atgtcaacca gtccatttct aatctgacgg aaaggggact gctccagtat
    15481 cgtactatcg aattaaatgg cgaaattgaa ttgctctttg atgctagttt ggccttggaa
    15541 cgtttggatg acctgtttgg agcagttcgt tcaagttcag accagctaac acctcaaaac
    15601 cagctcaagg atttggtgga aaccttccag caggagttgg gacgattgtt gacgcctttt
    15661 gagattgagg atttgaccaa gacactaaag gaagatggaa ccagtgctga cttgattaag
    15721 gaggctcttc gtgaagctgt tttgaatgga aaaccaaact ggaagtacat tcaggcgatt
    15781 ttgagaaact ggcgccatga aggaatcaag agtgtggctc aaattgaggc caagagagca
    15841 gaaagagaag caagcaatcc tcagttgaca caggtatctg cagatttcag aaatgccatg
    15901 gatctctgga aggattaatc catgcaagta ggcttgaaat ccgagtaaga tttgcaagct
    15961 gtgtataatt gtgatagaat aaatagaaaa taaattgaaa aaagaggtat gtgaaatgtc
    16021 acgtaaacca tttatcgctg gtaactggaa aatgaacaaa aatccagaag aagctaaagc
    16081 attcgttgaa gcagttgcat caaaacttcc ttcatcagat cttgttgaag caggtatcgc
    16141 tgctccagct cttgatttga caactgttct tgctgttgca aaaggctcaa accttaaagt
    16201 tgctgctcaa aactgctact ttgaaaatgc aggtgctttc actggtgaaa ctagcccaca
    16261 agttttgaaa gaaatcggta ctgattacgt tgttatcggt cactcagaac gccgtgacta
    16321 cttccatgaa actgacgaag atatcaacaa aaaagcaaaa gcaatctttg cgaacggtat
    16381 gcttccaatc atctgttgtg gtgaatcact tgaaacttac gaagctggta aagctgctga
    16441 attcgtaggt gctcaagtat ctgctgcatt ggctggattg actgctgaac aagttgctgc
    16501 ctcagttatc gcttatgagc caatctgggc tatcggtact ggtaaatcag cttcacaaga
    16561 cgatgcacaa aaaatgtgta aagttgttcg tgacgttgta gctgctgact ttggtcaaga
    16621 agtcgcagac aaagttcgtg ttcaatacgg tggttctgtt aaacctgaaa atgttgcttc
    16681 atacatggct tgcccagacg ttgacggtgc ccttgtaggt ggtgcgtcac ttgaagctga
    16741 aagcttcttg gctttgcttg actttgtaaa ataatcagta agtagcaaaa gctaggtgga
    16801 acagcattca gatgtctgtt acatttttta taggagagga aagattgaaa acaaaaattg
    16861 gattagcaag tatctgttta ctaggcttgg caactagtca tgtcgctgca aatgaaactg
    16921 aagtagcaaa aacttcgcag gatacaacga cagcttcaag tagttcagag caaaatcagt
    16981 cttctaataa aacgcaaacg agcacagaag tacagactaa tgctgctgcc cactgggatg
    17041 gggattatta tgtaaaggat gatggctcta aagctcaaag tgaatggatt tttgacaact
    17101 actataaggc ctggttttat attaattcag atggtcgtta ctcgcagaat gaatggcatg
    17161 gaaattacta cctgaaatca ggtggatata tggcccaaaa cgagtggatc tatgacagta
    17221 attacaagag ttggttttat ctcaagtcag atggggctta tgctcatcaa gaatggcaat
    17281 tgattggaaa taagtggtac tacttcaaga agtggggtta catggctaaa agccaatggc
    17341 aaggaagtta tttcttgaat ggtcaaggag ctatgatgca aaatgaatgg ctctatgatc
    17401 cagcctattc tgcttatttt tatctaaaat ccgatggaac ttatgctaac caagagtggc
    17461 aaaaagtggg cggaaaatgg tactatttca agaagtgggg ctatatggct cggaatgagt
    17521 ggcaaggcaa ctactatttg actggaagtg gtgctatggc gactgacgaa gtgattatgg
    17581 atggtgctcg ctatatcttt gcggcctctg gtgagctcaa agaaaagaaa gatttgaatg
    17641 tcggctgggt tcacagagat ggtaaacgct atttctttaa taatagagaa gaacaagtgg
    17701 gaaccgaaca tgctaagaaa atcattgata ttagtgagca caatggtcgt atcaatgatt
    17761 ggaaaaaggt tattgatgag aacgaggtgg atggtgtcat tgttcgtcta ggttatagcg
    17821 gtaaagaaga caaggaattg gcgcataaca ttaaggagtt aaaccgtctg ggaattcctt
    17881 atggtgtcta tctctatacc tatgctgaaa atgagaccga tgctgagagt gacgctaaac
    17941 agaccattga acttataaag aaatacaata tgaacctgtc ttaccctatc tattatgatg
    18001 ttgagaattg ggaatatgta aataagagca agagagctcc aagtgataca ggcacttggg
    18061 ttaaaatcat caacaagtac atggacacga tgaagcaggc gggttatcaa aatgtgtatg
    18121 tctatagcta tcgtagttta ttacagacgc gtttaaaaca cccagatatt ttaaaacatg
    18181 taaactgggt agcggcctat acgaatgctt tagaatggga aaaccctcat tattcaggaa
    18241 aaaaaggttg gcaatatacc tcttctgaat acatgaaagg aatccaaggg cgcgtagatg
    18301 tcagcgtttg gtattaagcg atgatttgaa agagggatgt gatagtagca ccctcttttt
    18361 ctttgtttta tgatagttca tcctcgagta aattcaagtt cttgctcgga aatgaagctt
    18421 atatagtaga ttgaatatag acaaatacct tgtgattggt aaaacatttt agaaattcat
    18481 ttacctttcc taatcgactt ggtttcatct tatttcaatc tattatagta ttggggaatt
    18541 tcttcaaacc acatcagctt ggtcagttct acctgcgacc tcaaaacttg tgctttggtc
    18601 aagctgggtt tagtttccta gtttgctgat ggatttccat tgactataag catccaaccc
    18661 tctttttgtc ttctaaagaa ttcttaaatt atcagtctat tgcaactttt ctcatataag
    18721 ttctttgtct tgctattggt tttccttagt agtatactaa ggtagtaatc attaagaagt
    18781 ggttacaaaa aataatgaat gaggtaaaga aaatggtaga attgaaaaaa gaagcagtaa
    18841 aagacgtaac atcattgaca aaagcagcgc cagtagcatt ggcaaaaaca aaggaagtct
    18901 tgaaccaagc tgttgctgat ttgtatgtag ctcacgttgc tttgcaccaa gtgcactggt
    18961 atatgcatgg tcgtggtttc cttgtatggc atccaaaaat ggatgagtac atggaagctc
    19021 ttgacggtca attggatgaa atcagtgaac gcttgattac actcggtgga agcccattct
    19081 ctacattgac agagttcctt caaaatagtg aaatcgaaga agaagctggt gaataccgta
    19141 atgttgaaga aagcttggaa cgtgttcttg ttatctaccg ttacttgtca gaacttttcc
    19201 aaaaaggttt ggatgtcact gatgaagaag gtgacgatgt gacaaacggt atctttgcag
    19261 gcgctaaaac tgaaacagat aaaacaattt ggatgcttgc agccgaactt ggacaagcac
    19321 ctggtttgta agaaataagg aaacatagat aaatctgtag gaaatttctt acagattttt
    19381 tctattttgt aagctattcc aaaccagtct ttttttgagt tttttgataa aatagtacta
    19441 tcagtgaaaa ggatggaagc atgactaaga aaatcgtagc tatttgggcc caggatgaag
    19501 agggtgtgat tggtaaggaa aatcgtctgc cttggcattt gccagcagaa ttgcagcact
    19561 ttaaagaaac aactctgaat catgctatct tgatggggcg tgtgaccttt gatgggatgg
    19621 ggcgtcgctt gcttccaaaa cgggaaacct tgattttgac gcgtaatccg gaagaaaaga
    19681 tagatggggt tgctactttt caggacgtcc agtctgttct tgactggtat caggctcaag
    19741 aaaagaatct ctacattatc ggtgggaagc aaatttttca ggcttttgaa ccttaccttg
    19801 atgaagtgat tgtcactcac attcatgctc gggtggaagg agatacctat ttccctgaag
    19861 aacttgactt gtctcttttt gagacagttt caagcaaatt ttacgccaaa gatgagaaga
    19921 atccttatga ttttaccatc caataccgca agagaaagga agtctaatgg aacggagttt
    19981 atttggtcta ttcacggctt ttctgtgttt catctgcttt ctggcaggag cacaagcttt
    20041 tcgtaaaaag cgttacgggc tttctatcct cctttggtta aatgccttta ccaatctggt
    20101 caatagtatt catgcttttt acatgacctt attttagata gaatgacagt atagaatgga
    20161 aggaaatcat gtctacaaat agaaaaaatg atatgatggt ttattgctca ttttgtggca
    20221 aaaaccaaga agaagtacaa aaaataattg ctggcaacaa tgcttttatt tgtaatgaat
    20281 gcgtggagtt agctcaggaa atcattcgag aagaattggt tgaggaagtc ttggcagact
    20341 tgtctgaggt gccaaaacca attgaactcc tccatatctt gaaccactat gtaattggtc
    20401 aagatcgtgc caagcgtgcc ttggcagtgg cggtttataa ccactacaaa cgcatcaatt
    20461 tccacgatac acgcgaagag tcagaagatg tggatttgca gaagtcaaac attttgatga
    20521 ttggcccaac tggttcaggg aaaactttcc ttgcccagac cttggctaag agcttgaatg
    20581 taccttttgc tattgcggat gcgacagctc tgacggaggc tggttatgtg ggtgaggatg
    20641 tggaaaatat actcctcaaa ctcttgcagg ttgctgactt taacatcgaa cgtgcagagc
    20701 gtggcattat ctatgtggat gaaattgaca agattgccaa gaagagtgag aatgtgtcta
    20761 tcacacgtga tgtttctggt gaaggggtgc aacaagccct tctcaagatt attgagggaa
    20821 ctgttgctag cgtaccgcct caaggtggac gcaaacatcc acaacaagag atgattcaag
    20881 tggatacaaa aaatatcctc ttcatcgtgg gtggtgcttt tgatggtatt gaagaaattg
    20941 tcaaacaacg tctgggtgaa aaagtcatcg gatttggtca aaacaataag gcgattgacg
    21001 aaaacagctc atacatgcaa gaaatcatcg ctgaagacat tcaaaaattt ggtattatcc
    21061 ctgagttgat tggacgcttg cctgtttttg cggctcttga gcaattgacc gttgatgact
    21121 tggttcgcat cttgaaagag ccaagaaatg ccttggtgaa acaataccaa accttgcttt
    21181 cttatgatga tgttgagttg gaatttgacg acgaagccct tcaagagatt gctaataaag
    21241 caatcgaacg gaagacaggg gcgcgtggac ttcgctccat catcgaagaa accatgctag
    21301 atgttatgtt tgaggtgccg agtcaggaaa atgtgaaatt ggttcgtatc actaaagaaa
    21361 ctgtcgatgg aacggataaa ccgatcctag aaacagccta gaggtgacta tggaacttaa
    21421 tacacacaat gctgaaatct tgctcagtgc agctaataag tcccactatc cgcaggatga
    21481 actgccagag attgccctag cagggcgttc aaatgttggt aaatccagct ttatcaacac
    21541 tatgttgaac cgtaagaatc tcgcccgtac atcaggaaaa cctggtaaaa cccagctcct
    21601 gaactttttt aacattgatg acaagatgcg ctttgtggat gtgcctggtt atggctatgc
    21661 tcgtgtttct aaaaaggaac gtgaaaagtg ggggtgcatg attgaggagt acttaacgac
    21721 tcgggaaaat ctccgtgcgg ttgtcagtct agttgacctt cgtcatgacc cgtcagcaga
    21781 tgatgtgcag atgtacgaat ttctcaagta ttatgagatt ccagtcatca ttgtggcgac
    21841 caaggcggac aagattcctc gtggtaaatg gaacaagcat gaatcagcaa tcaaaaagaa
    21901 attaaacttt gacccaagtg acgatttcat cctcttttca tctgtcagca aggcagggat
    21961 ggatgaggct tgggatgcaa tcttagaaaa attgtgagga aaagaaaatg gcaaaaacaa
    22021 ttcatacaga taaggcccca aaggctatcg gaccctatgt tcaaggaaaa atcgttggca
    22081 accttttgtt tgctagcggt caagttcccc tatcccctga aactggggaa attgtaggag
    22141 agaatatcca agaacagaca gagcaagtct tgaaaaacat cggtgctatt ttggcagaag
    22201 caggaacaga ctttgaccat gttgtcaaaa caacttgctt cttgagcgat atgaacgact
    22261 ttgttccttt taatgaggtt taccaaacgg ccttcaaaga ggaattccca gctcgttcag
    22321 ctgtggaggt agctcgtctt cctcgtgatg taaaagtcga aattgaagtc atcgcagaga
    22381 ttggataagc tagttgaagt ttggtgttgc caaacttctt ttgatataag gagaaaaaga
    22441 tgacaaagaa acaacttcac ttggtgattg tgacagggat gagtggcgca gggaaaactg
    22501 tagccattca gtccttcgag gatctaggtt atttcaccat tgataatatg ccgccagctc
    22561 tcttgcctaa gtttttgcag ctggttgaaa ttaaggaaga taatcctaag ttggctttgg
    22621 tagtggatat gcgtagccgt tctttctttt cagagattca agctgttttg gatgagttgg
    22681 aaaatcaaga tggtttggat ttcaaaatcc tctttttgga tgcggctgat aaggaattgg
    22741 tcgctcgtta caaggaaacc agacggagtc acccactagc agcagacggt cgtattttag
    22801 atggaatcaa gttggaacgt gaactcttgg cacctttgaa aaatatgagc caaaatgtgg
    22861 tggatacgac tgaactcact ccacgtgaac tgcgcaaaac ccttgcagag cagttttcag
    22921 accaagaaca agcccagtct ttccgtatcg aagtcatgtc tttcggattt aagtatggaa
    22981 tcccgattga tgcggacttg gtctttgatg tccgtttctt gccaaatccc tattatctac
    23041 cagaactgag aaaccaaacg ggtgtggatg aacctgttta tgattatgtc atgaaccatc
    23101 ctgagtcaga agacttttat caacatttat tggccttgat tgagccgatt ctgccaagtt
    23161 accaaaagga aggtaagtcc gttttgacca ttgctatggg atgtacgggt ggacaacacc
    23221 gtagtgtggc atttgctaaa cgcttggcgc aggacttatc caagaattgg tctgttaatg
    23281 aagggcatcg cgacaaagac cgcagaaagg aaacggtaaa ccgttcatga gaaaaccaaa
    23341 gataacggtg attggtggag ggactggaat tcccgtcatt ctaaaaagtc tgcgggaaaa
    23401 agatgtggaa atcgcagcta tcgtgacggt ggcagatgat ggtggttctt caggtgaact
    23461 ccgaaaaaat atgcaacagt tgacaccgcc aggtgatctt cgtaatgtcc ttgtggccat
    23521 gtcggatatg cctaagtttt atgagaaggt ctttcagtat cggttctctg aggatgccgg
    23581 agcctttgct ggccatccat tgggaaatct catcattgct ggcttgtcag aaatgcaggg
    23641 ttcaacctat aatgccatgc agttattgag caaatttttc catacaacag ggaaaattta
    23701 tccttccagt gaccatcctt tgacccttca tgcagtcttt caggatggga cagaagtggc
    23761 tggagagagt catattgtag accatcgagg cataattgac aatgtctatg tgaccaatgc
    23821 cctaaacgat gatacgcctc tggccagccg tcgagtagtg cagaccatcc ttgaaagtga
    23881 catgattgtc ctagggccag gttccctctt tacctctatt ttgcccaata tcgtgattaa
    23941 ggaaattggg cgggctcttt tggaaactaa ggcagaaatt gcctatgtct gcaatatcat
    24001 gacccaacgt ggggagacgg aacactttac agatagcgac cacgtggaag tcttgcatcg
    24061 tcaccttggt cgccctttta tcgacactgt cttggtgaat attgaaaaag tgcctcagga
    24121 atacatgaat tccaaccgtt ttgatgaata cttggtgcaa gtggaacacg attttgtagg
    24181 tctttgtaag caagtttcgc gcgtgatttc atctaacttc cttcgtctgg aaaatggcgg
    24241 tgccttccac gatggagatt tgattgtgga cgagttgatg cgcattatac aggtgaaaaa
    24301 atgagtttca cagtagcagt aaaagaagaa atcctgggcc aacaccatct gagccggcat
    24361 gaattatctg ccattatcaa aatgtctggt agcatcggtc tctcgacttc gggcttgact
    24421 ttgtcagttg tgacagaaaa tgccaaactg gcccgtcacc tctatgagtc ctttctccat
    24481 ttctatgaaa tcaaatctga aattcgtcac caccaacgga gcaatcttcg caagaatcgc
    24541 gtctataccg tttttacaga tgaaaaggtg caggatttgt taagtgattt gcacttggca
    24601 gactctttct ttggcctgga aacaggtatt gatgaggcga ttttatcgga tgaggaagca
    24661 ggtcgtgcct atctctgtgg cgctttcttg gcaaatggga gcattcgtga ccctgagtca
    24721 ggcaagtacc agttggaaat cagttctgtt tatctggacc acgcgcaagg aattgcctcc
    24781 cttctccaac agtttttact ggatgccaag gtgcttgagc gcaagaaggg ggctgtgacc
    24841 tatctccagc gagcagaaga cattatggac ttcttgatag taatcggagc catgcaggca
    24901 cgtgatgatt ttgagcgggt taagattttg cgagaaaccc gtaacgacct caatcgggct
    24961 aataatgccg agacagctaa tatcgctcgg acagtttctg ccagcatgaa gaccattaac
    25021 aatatcagta aaataaaaga tatcatgggc ttagaaaatt tgccagtaga tttgcaggaa
    25081 gtggcgcaac tgaggattca gcacccagac tactctatcc agcagttagc agatagtctc
    25141 agcacccctc tgaccaaaag tggtgtcaat cacagactca gaaaaatcaa taagatagcg
    25201 gatgaattat aaaaccacga atcctatgtg actcgtggtt cttttttata aactggtaga
    25261 gtgttttggt tgtacttttt gttcagggtc aatgtagttg atagcgttgt tgacagcagt
    25321 tggagcttct ccgaggcctg tcgcaatcag atcaattttt ccgtcatagt agcagcagtc
    25381 accgatagca tagatacctg cttggctgga ttcctgtttg ctgttgacga taatcttgtg
    25441 acggttgagg tcgagccccc agtttttaag gttaccgaca gaagatttga aaccatagtt
    25501 gacaaagagg tggtctaggt caatggtttc agtttcatca gatttgactt ttgtgatttc
    25561 aagtttatca agtgtttttc catttccaag gagttggcta ggggcgaatg gtgtcttgat
    25621 ggttacagat gattcttgca aggcttgaac actgtgttcc aaggcacgga aattatctct
    25681 gcggtgaaca agggtagttg gtgcgatttt ttcaaaagcc aaagtccaat ccacagccga
    25741 gtctccccca ccaagaatcg tcactttttt accagcgtat tgctgaatgt tagaaacgtg
    25801 gtagtggata ttttcatagc cctcaacccc ttcaagttcc agcggacgtg gtttgaaggc
    25861 accgccaccc atagcgatga taactgtttt agtcaggtga cttcctttag aagttgtgat
    25921 ggcaaattct tcttcttgtt tgtcaatctc aagaaccgtt tcattgagat gaataggggt
    25981 atcaaatcca tttagctgtt caatcaagcg gttagtcaac tcttctccag tcaggtttgg
    26041 gaagcctggt acgtctagga tttccttttc agggtagaga atagcaggtt gtccacctag
    26101 ctggggaaga gagtcgatga tttgaacctt ggcttggcgt aggtgggcat aaaaggctgc
    26161 aaaaagcccg acaggaccac cacccacaat ggtaatatca tagagttgag acatagtttc
    26221 tcctttgttt tttctagtca gtttatttta tcatattttt gttcaatgta aaatggacta
    26281 ggatagggaa aaaaatgcca gaaaatggta taataggacg aaagtgtttt gatagggagg
    26341 atatcaggga tgaattttca acaattatcc aatttgcaat attggaccag tttgtttgca
    26401 agtccatgga cgatagctat caatctgatt gatattttga ttgttgctta tattttatac
    26461 cattttacaa aagctattgc aggaaccaag attatgattt tggtacgtgg agttttggta
    26521 tttattttag ctcaaatcct tgcaaatatg attggtttga ctacgatttc ttggttaatc
    26581 aatcaaatta ttacttatgg ggttattgcg gcggttgtta tcttctctcc agagattcgg
    26641 actggtttgg aacgtttggg aagagcgaca gatttctttt ccaatgcccc tattagtgct
    26701 gaggaacaga tgattcgtgc ctttgttaag tctgttgaat acatgagtcc tcgtaaaatc
    26761 ggggccttgg ttgctattca gcgtgtacgt accttgcagg agtatatttc gacaggaatt
    26821 cccttggatg ctaagatttc tgcagaactt ctcattaaca tttttattcc caacactccc
    26881 ctacatgacg gtgcggtgat tatcaaagaa gaacgtatcg ctgtgacgtc tgcctatctg
    26941 cccttgacaa aaaacacagg gatttccaag gaatttggga ccagacaccg ggcggctatc
    27001 ggtttatcag aagtctcaga tgccttgact tttgtcgtat cagaggaaac gggaggaatt
    27061 tcgataacct ataatggaag gtttaagcac aacctaacac ttgatgaatt tgaaacagaa
    27121 ttacgtgaaa tcttacttcc aaaagaggaa gtaggtctta gttttaaaga acgattgcta
    27181 ggaggatgga aacatgaaaa aaaatagttt atatatcata tcctcactct tttttgcttg
    27241 tgtcttattt gtctatgcta cggcgacgaa ttttcaaaac agtaccagtg ctaggcaggt
    27301 aaaaacggaa acctatacta atacagtaac aaatgtccct attgacatac gctataatag
    27361 tgataagtat tttattagcg gttttgcttc agaagtatca gtggtcttga ctggtgcaaa
    27421 tcgcctatcg ctagctagtg aaatgcaaga aagtacacgt aaattcaagg ttactgctga
    27481 cctaacagat gccggtgttg gaacaattga agttcctttg agcattgaag atttacccaa
    27541 tgggctgacc gctgtggcga ctccgcaaaa aattacagtc aagattggta agaaggctca
    27601 gaaggataag gtaaagattg taccagagat tgaccctagt caaattgata gtcgggtaca
    27661 aattgaaaat gtcatggtgt cagataaaga agtgtcgatt acgagtgacc aagagacatt
    27721 ggatagaatt gataagatta tcgctgtctt gccaactagc gaacgcataa caggtaatta
    27781 cagtggttca gtacctttgc aggcaatcga ccgcaatggt gttgtcttac cggcagttat
    27841 cactccgttt gatacaataa tgaaggtgac tacaaaacca gtagcaccaa gttcaagcac
    27901 atcaaattca agtacaagca gttcatcgga gacatcttcg tcaacgaaag caactagttc
    27961 aaaaacgaat taaaaataga aaaggatttt ataacaatgg gtaaatattt tgggactgat
    28021 ggagtccgtg gagaagctaa cctagaacta acaccagaat tagcctttaa actaggacgt
    28081 tttggaggct atgttcttag tcaacatgaa acggaagcgc cgaaagtctt tgtaggacgt
    28141 gacacacgta tttcagggga aatgctagaa tcggccttgg tggcaggtct cctttcagta
    28201 gggattcacg tatacaaact tggtgtcctt gcaacaccag cagtagctta cttggttgaa
    28261 actgaaggag caagtgctgg tgtcatgatt tctgctagcc acaatccagc tcttgataac
    28321 ggaatcaagt tctttggcgg tgatggcttc aaactagatg atgaaaaaga agcagaaatt
    28381 gaagccttgc tagatgctga ggaagacact cttcctcgtc caagtgcaga aggcttagga
    28441 attttggtag attatccaga aggcttgcgt aagtatgaag gataccttgt ttcaactgga
    28501 actcctcttg atggaatgaa ggttgccttg gatacagcta atggagcagc ttctaccagt
    28561 gcccgtcaaa tctttgcaga ccttggtgcc caattgacgg ttatcgggga aacaccagac
    28621 ggtcttaaca tcaaccttaa tgttggttca acacatccag aagcccttca agaagtggtc
    28681 aaagaaagtg ggtcagctat tggtttggcc tttgatggag acagtgaccg cttgattgct
    28741 gttgatgaga atggtgacat cgtcgatggt gacaagatta tgtacatcat cggaaaatac
    28801 ctttctgaaa aaggacaatt ggctcaaaat acaattgtga caactgttat gtctaacctt
    28861 ggtttccaca aggccttgaa tcgcgaaggt attaacaagg cagttactgc agttggtgac
    28921 cgctacgttg ttgaagaaat gagaaaatca ggttacaacc ttggtggtga acagtctggt
    28981 cacgttatct tgatggatta caataccaca ggtgatggtc aattatcagc aattcaattg
    29041 actaaaatca tgaaggaaac tggtaagagc ttatcagagt tggcggcaga agtaacgatt
    29101 tatccacaaa aattagttaa tatccgagtg gaaaacgtca tgaaggaaaa ggccatggaa
    29161 gtgccagcta tcaaggccat catcgagaag atggaagaag aaatggcggg gaacggccgt
    29221 atccttgttc gtccaagtgg aacagagccc ctcttgcgtg ttatggcaga agcgcctaca
    29281 acagaagaag tggactacta tgttgatact atcacagatg tagttcgtgc tgaaattggg
    29341 attgactaaa tcctagaaaa ctagatgaaa ataaaaaatt atggtacaat agcttataat
    29401 attgtagccg atggagaaag acatgaatct taaacgtgaa caagaatttg ttagtcagta
    29461 tcattttgat gcacgtaatt ttgaatggga aaatgaaaat ggagctcctg aaaccaaggt
    29521 ggatgtgaat ttccaattgc tccaacatga ccaagaaaat caagtgactt ccttgattgt
    29581 cattttgagc tttatgattg tatttgataa gtttgtcatt agtggaacaa tctcacaagt
    29641 aaaccatatc gatggccgta ttgtcaatga accaagtgaa ttgaaccaag aagaggtgga
    29701 aaccttggct cgtccatgtt tgaacatgct caatcgtttg acttacgaag tgactgaaat
    29761 tgcattagac ttaccaggaa ttaatttgga gttctaatat gaaattagct gttttcacag
    29821 attcctctgc ttatctcagt gaagagacct tgcaaagaga agacttgttt gtcttggata
    29881 ttcctgtcaa tattgatggt gaggaatatg tcgaaggcat caatctgtct gctgaggaat
    29941 tttaccaaaa aatggctcag gcttctgaat tgcctaagac cagtcaacca agtattgcca
    30001 agttagatga gatcttaact tcgctcaaag aacaaggcta tacacatgcc ttggggcttt
    30061 tcctatcttc tggaatttca ggtttttacc aaaatatcca gtatatggtc gatgactatg
    30121 agggcttaac cattgctttc ccagacactt tgattacaag tgctccccta ggcatcatgg
    30181 ttgaaagcgt ctttaattgg cgtgatcagg gcgatgattt tgccagcatt caggataagc
    30241 tagccattca aatcagccgt acgtcagcct ttatcatggt agatgacttg gatcatttgg
    30301 taaaaggtgg acgcctttca aatggggctg ccattttggg caatttgctt agcattaagc
    30361 caatccttta ttttaacgat caaggtgtga ttgaagttta cgaaaaagtt cgtactgaaa
    30421 agaaggccac caagcgctta attgaaatta tcaaggaaac aacggcttca ggtcaatacc
    30481 gggtcattgt cattcatggg aatgctcctg aaaaggctga agaattgcgt cagcacttgc
    30541 ttgattttgg cttgggttcg gatgtttcac ttgctacatt tggtagtgtc attggaacgc
    30601 acctaggagc aggaagtatt gctctgggtt atattccagt gatttagttg gcgcttctag
    30661 gctagttaag atgccttgta tcttagaaat tgaacacgga ctacctgcct cttgaaaaaa
    30721 gatgcctgtc ttgcctttcg tggaaagtca gtgccattcc ctatttttca gaggcagcta
    30781 aggtcctttg tatcttgata attgaacacg cctggaaccc tgtgtgaaaa agatagttct
    30841 tccaagaagt agacacttct tgattggaat tcctattttc actttgtgtt cttacggact
    30901 ttgtatctta gacaggagta gagatgagta ttcgagtaat tattgccggt tttaagggaa
    30961 agatgggcca ggctgcttgt cagatggtat tgactgatcc agacttggac ttggtggcag
    31021 ttttggatcc ttttgagtct gagtcagaat ggcagggtat tcctgttttc aaggataagg
    31081 ctgatttagc tggttttgaa gcggatgtct gggtagattt tactactcca gctgttgcct
    31141 acgaaaatac acgttttgct cttgaaaatg gctttgctcc agtagttgga acgactggtt
    31201 tcacgagtga agaaattgca gagctaaaag aattttctcg tgcccaagac ttgggtggcc
    31261 tgattgcccc taactttgcc ttgggtgctg tcttactcat gcaatttgcg acgcaggctg
    31321 ccaaatattt cccaaatgtg gagattattg agctccatca tgacaagaaa aaggatgctc
    31381 cgagtggaac agccattaaa acagctgagt tgatggcaga ggttcgagag tccattcagc
    31441 aaggcgcagc agatgaggaa gagctgattg ctggtgctcg tggtgctgac tttgatgata
    31501 tgcgcatcca ctcagttcgt ttgccaggct tggtagctca tcaggaagtc atctttggca
    31561 atcagggaga agggttgacc ctccgtcatg actcctatga tcgcatctcc ttcatgacag
    31621 gagtcaattt gggaattaaa gaagttgtca agcgtcatga gcttgtctat ggattagaac
    31681 acttattatg agattaacgc aaatgccttc tgaatttcag aaggctttac cagtattaga
    31741 aaaaattaaa gaagcaggct ttgaggctta ttttgttggg ggctctgttc gagatgccct
    31801 tctccatagc cctatccacg atgtggatat tgcgacgtct tcttatccag aagagaccaa
    31861 gcagattttt ccgcgaacag ccgatatcgg aatcgagcat ggaaccgtct tggtcttaga
    31921 tggggatgag gagtatgagg taacaacctt tcggacagag gatgtctatg tggactatcg
    31981 cagacccagt gcggtttcct ttgtacgatc gctagaagaa gacctcaaac gccgtgattt
    32041 cacagtcaac gcctttgcct tggatgagac aggagaaatc gttgacttgt tccatggttt
    32101 agaagatttg gaaaagcaag tcttgcgagc agttggagtg gctagtgagc gtttcaacga
    32161 agatgcttta cggattatgc gtggtttccg ttttcaggct agtcttggtt ttgcacttga
    32221 gccagaaaca tttaaagcta tgaagacctt gacgccgctt ttggagaaaa tttctgtaga
    32281 gcgtaccttc gtcgagtttg ataaactctt gctggctcca ttttggagaa ggggcttggc
    32341 ttccatgatt gagagtcaag cttatgacta tctccctgat atggcatcta gccaggacaa
    32401 gctcaacaga ctgtttgatt tggagactga ttttactttt gaatcctctg aacaagcctg
    32461 ggcggcttta ctatgggctt tggagattga aaatgcgcag tcatttttga aatcttggaa
    32521 gacctcacgc cagtttgcca agcaagttca ggatttgctg attattttgg ctctgcgtga
    32581 aaatggagaa ttgagtaagc gagattgtta tcgctttgac atagatttgc ttttacaggc
    32641 tgaaaatctt cgtcaagctc aaggaaaaga agtcaaccca caagccatca cagaaaaata
    32701 ccaaagcttg actatccatg acaagaaaga gattcagatt aatggcgata ttttgatcaa
    32761 ggaatatggc tatcagccag gcccagactt gggagagatt ttaacagaga ttgagtttgc
    32821 cattgtcgat ggagaattgg agaataatcg tgaagccatc catgcttacc tgagggagaa
    32881 aaaatgagtg attttatcgt tgaaaaacta agtaaatccg ttggtgacaa gaccgttttt
    32941 agggatattt cctttattat ccatgactta gacagaattg gtttaatcgg tgtcaatggg
    33001 actggcaaga ccaccctttt ggacgtcctt tctggtgttt ctggatttga tggggatgtc
    33061 agtccttttt cagctaaaaa tgattaccag attggttact tgactcagga tcctgatttt
    33121 gatgatagaa agacagtttt ggatacggtt ctatctagtg aactcaagga aatccagctc
    33181 attcgtgagt atgaattgat tatgctcgac tatagtgagg acaagcaggc gcgtttggaa
    33241 cgtgtcatgg cagagatgga ctctctccaa gcttgggaaa tcgaaagtca ggtcaagacc
    33301 gttcttagca aattgggcat tcaagactta tctactcctg ttggggaatt gtcaggtggt
    33361 ctgagaagac gggtacagtt ggcacaagtc ttacttggca accacgacct cttgcttttg
    33421 gatgagccga ccaaccatct ggatattgcg attattgagt ggctgaccct ctttttgaaa
    33481 aattctaaga agaccgtcct ttttatcact cacgatcgtt atttcttaga cgctttgtca
    33541 acacggattt tcgagttgga tcgtgcaggc ttgaccgagt accagggcaa ttaccaggac
    33601 tatgttcgcc taaaggcgga acaggatgag cgcgacgcgg ctcttcttca caaaaaagaa
    33661 caactctaca aacaagaact ggcctggatg cgcagacaac cgcaggcgcg tgcgaccaag
    33721 cagcaggccc gtatcaatcg attccatgat ctgaaaaagg aagtttcagg cagtagtgct
    33781 gagacagact tgaccatgaa ctttgaaacc agtcggattg ggaagaaagt catcgagttt
    33841 caggctgttt cctttgccta tgaaaacaag cctattttgc aagattttaa tctcttggtg
    33901 caggccaaag accgtatcgg aatcgttggg gacaatggtg ttggaaaatc aaccctgctc
    33961 aacttgattg caggaagtct tgagccaact aaaggacaag ttgtgattgg ggaaactgtt
    34021 cgcatcgcct atttctctca gcaaattgag ggtttggatg aaagcaagag agttatcaat
    34081 tacctgcagg aagtggcaga agaggtcaag accagtggcg gttctacgac ttcgattgca
    34141 gagttgctgg agcagttcct tttcccacgt tcgacgcatg gaaccttgat tgagaaattg
    34201 tcagggggtg agaaaaaacg tctttatctc ctcaaactgc ttttggaaaa accaaatgtt
    34261 cttcttttag acgagccaac caatgaccta gatattgcaa ctttgacagt cttagagaat
    34321 ttcttgcaag gttttgcagg tcccgtttta acagtcagtc acgaccgcta tttcttggat
    34381 aaggtagcga ccaagattct cgcttttgag gatagcaaga ttcgtccttt ctttggtcat
    34441 tacaccgact atcttgatga aaaagctttt gaaacagata tggccaatca agtgcaaaag
    34501 gtcgaaaagg aaaaagtggt caaggttcga gaagacaaga aacgtatgac ctaccaagaa
    34561 aagcaggagt gggcaagtat tgaaggtgat attgaaacct tggaaaaacg tatcgctgct
    34621 attgaagagg aaatgcaggc taacggctct gactttggta agctggctac tctccaaaaa
    34681 gaattggatg agaaaaatga agcactcctt gaaaaatacg aacgctatga gtatctcagt
    34741 gaatttgata gttaagacta ttcgaaaatc aaagtgcaaa ctatgtcggc ttcacattgc
    34801 cgactcaagt acagcctgtg accggcttcc taatttgcac tttgattttt attgagtata
    34861 aaagaataga aaaatcccgt ctcatgaaag gatttctcta ttcttttttg gtttcttgat
    34921 gaaagatatt ttgccaggtt tcgtggcgac gccagatact ggtatggatg ataccatcta
    34981 actcatagca gatgagtttg gttttttgac tgatggaagt aatttgaatg tccttgatag
    35041 cagaatttaa ctcttttttg gtcttataag cctctttatc catctgctct ccatcttggc
    35101 gaatatagac aaaatcatca gtcaagagtt cttctagttg gtttccttgg tcaatcaatt
    35161 gttcacgcat aagcaatttt ttatagacat tgtctaaaat ttcatcctca gagataggtg
    35221 ctggttcgat atggacatcg gtatcaaaga cgccaaaacg ctcctccagc atagactcga
    35281 cctgatccgc gatttcatgg ctttcaaaaa cagacaagtc aggattcatc tctagtgtaa
    35341 tatccaggta gatgttgcta ccgtaggtgc gacctctttg cgatttgacc ttgctgattt
    35401 tgggaatttc catgatagcc ttttggtagt cctcgagcag gcggtcgtca aagccatctg
    35461 aaagactaaa ggaagactcg atgaagatat cataggcagt cttcaagata aagaaagtga
    35521 tgatgatagc aaccagttta tccacaatcg gataattgaa actactagct aggatggcaa
    35581 tggcggttcc aagtgaggta acagcgtcag aaagattgtc cttagcagct gccttcagcg
    35641 ccttggagtt ggatttctta ctgaggcgag tattgtagag atagaccaca aacataatcg
    35701 ctgcagaaat gattcctaga gttgcaccaa gaggatcaat gaccgtttct tcccgactga
    35761 gaatcttttg aatggtatct cttagaacat cgaaaccgac atagaacatg atgatagaag
    35821 tgatcaagct tgccaaatct tcaatcttcc aatgaccaaa acggtggtca cggtctgcag
    35881 gctggcgcgc catccgaatc ccgattaaga gggccacatt tccaatgatg tccgatacgt
    35941 tattaaaacc atcggccacc aaactggatg aatgaaggag atgaccagct gctaatttgg
    36001 ctgcagacaa gatcaaatag gtcgaaatac tgataatggc tccacgctca gctaacttga
    36061 gatttgagat agattgcttc attcaacttc ctttctagtc acatagcatt ctaccattat
    36121 actggttttc aaggaaaaat tcaaataagg cagtttttac tctttgaaaa ctccccaaaa
    36181 tttggtataa tggtactact caagggagta gctggcagaa acctgtgata gtgtcgtcat
    36241 tccgaatttt atgctgaaaa gtatgctttc cggccctatc ttaaacagcg agacttgtta
    36301 tgattaacag gtctcttttt gtttgtcgtg aaaagatatg acagaaagag agtctgtttt
    36361 gcacacaatg tcaaggagga gacacatgtc aaaagaacaa aaacgccaag cgttttatac
    36421 tcagagccct gaagaggtct tgcaggctgt ggatgcgacc gagcaaggtt tgtcatcaag
    36481 tgaggcggaa aagcgccttg ctgaatttgg gcacaatgaa ctcgaagaag gcgagaaacg
    36541 atcaatcttg gtcaaattca tcgagcaatt taaggatttg atgattatca tcctagttgc
    36601 ggcagcaatc ttgtcagtcg tgacttctgg tggggaagat atcgcagatg ccattatcat
    36661 cctagctgtg gttatcatca acgctgcctt tggtgtttac caagaaggaa aagctgaaga
    36721 agctattgaa gccctcaaat ccatgtctag tccagttgcc cgcgttcttc gtgatggaca
    36781 catggcagag attgactcta aagaattggt accaggcgat atcgttgccc ttgaagcagg
    36841 tgacgtggta ccagcggacc tacgtttgat agaagccaac tctcttaaaa ttgaagaagc
    36901 agccttgaca ggtgaatctg taccagtcga aaaagacttg tcagtcgagc ttgcgacaga
    36961 tgctggtatt ggtgaccgtg tcaacatggc cttccaaaac tcaaacgtaa cctatggtcg
    37021 tgggatgggt gttgttgtca atacaggtat gtacactgaa gttggtcata ttgctggtat
    37081 gcttcaagat gcggatgaga ctgatacacc actcaaacaa aatttgaaca acctttctaa
    37141 ggtcttgacc tatgctatct tggtcattgc ccttgttact tttgtagtgg gtgtcttcat
    37201 tcaagggaaa aatccacttg gtgagttgtt gacttctgtt gcccttgccg ttgcagccat
    37261 tccagaagga cttcctgcta tcgttaccat cgttctttct cttggtactc aagttttggc
    37321 caaacgacat tccatcgttc gtaagttgcc agcagttgaa acacttggtt caactgaaat
    37381 catcgcttct gataagactg gtacgctgac tatgaacaag atgacagtcg aaaaagtctt
    37441 ctacgatgcg gttctacatg actcagctga tgatattgaa ctaggtcttg aaatgccact
    37501 acttcgttca gttgtcttgg ccaatgatac gaaaatcgat gtggaaggta acttgattgg
    37561 tgacccaacc gaaacagcct ttatccaata tgccttggac aagggctatg atgtcaaagg
    37621 tttcttagag aaatatcctc gtgtagctga attgccattt gactctgacc gtaagctcat
    37681 gtcaacagtt cacccattgc cagatggtcg tttccttgta gcagtcaagg gtgcgccaga
    37741 ccaactctta aaacgttgtc ttcttcgtga taaggctggg gatattgctc cgattgatga
    37801 gaaggttaca aatctcattc atacaaacaa ttctgaaatg gctcatcaag ccttgcgtgt
    37861 ccttgcaggt gcttataaga ttatcgatag tattccagaa aatctcactt ctgaagagct
    37921 tgaaaatgat ttaattttta ctggtttgat tgggatgatt gaccctgaac gtcctgaagc
    37981 cgctgaggct gttcgtgtgg ctaaggaagc gggaatccgt ccaattatga ttacaggtga
    38041 ccaccaagac acagcggaag ccattgccaa acgtttggga atcattgacg caaacgatac
    38101 agaaggtcac gttttaactg gtgctgaact caatgaactg tcagatgaag aatttgaaaa
    38161 agtcgttggt caatactctg tttatgcccg tgtgtctcca gaacacaagg ttcgtatcgt
    38221 caaggcttgg caaaaacaag gtaaagtcgt tgccatgaca ggtgacggtg tcaatgacgc
    38281 gccagctctg aaaacagccg atatcggtat cggtatggga atcactggta cagaggtttc
    38341 taagggggct tctgatatga ttcttgcaga tgataacttt gcgactatta tcgtcgcagt
    38401 ggaagaagga cgtaaggtct tctcaaacat tcaaaagact attcagtacc tactttctgc
    38461 taatactgct gaagtattaa ccatcttcct atcaaccttg tttggttggg atgtcttaca
    38521 gccggttcat cttttgtgga ttaacttggt aacggatacc ttcccagcta tcgctcttgg
    38581 tgttgaacct gcggaacctg gtgtcatgaa tcataaacca cgtggacgca aggcaagctt
    38641 cttctcaggt ggtgttttga gttctatcat ttatcaaggt gtactccaag cagctcttgt
    38701 tatgagtgtt tatggccttg cgattgctta cccagttcat gtgggtgaca atcatgctat
    38761 tcatgcagat gccctaacga tggcctttgc aacccttggt ttgattcagc tcttccatgc
    38821 ctacaatgtc aagtctgtct accaatccat cttgacagtt ggcccattca agtctaagac
    38881 ctttaactgg tccatcttgg tatcctttat ccttctcatg gcaacaatcg ttgtagaacc
    38941 gcttgaagga atcttccacg taaccaaact agacttgtca caatggggaa ttgttatggc
    39001 tggaagcttc tcaatgatta tcatcgtcga aatcgttaag tttattcaac gcaaactcgg
    39061 ttttgacaag aatgcgattt aagggaggtt tcggatgtca gcatatcaat taccgaccgt
    39121 atggcaggat gaagctagta atcaaggagc ttttacgggg ctaaacagac caacagcagg
    39181 tgcccgtttc gaacaaaact tgccaaaagg agaacaagct tttcagcttt attcactggg
    39241 aacaccaaat ggtgtgaagg ttactatctt attggaagaa ttactagaag ctggttttaa
    39301 ggaagcggct tacgacttgt ataagattgc tatcatggat ggggatcaat tcggctcaga
    39361 ctttgtgaag ctcaatccaa attccaagat tccagcctta ttggaccagt caggtactga
    39421 aaacgtaaga gtctttgagt ctgctcatat tcttctttac cttgctgaga aatttggagc
    39481 ctttttacca agtaatcctg tggaaaaggt agaagttttg aattggctat tctggcaagc
    39541 aggtgcagca ccttttctag gtgggggatt tggacatttc ttcaattatg ctcctgaaaa
    39601 attggaatat cctattaacc gttttacgat ggaagtgaaa cgccagttgg atttattgga
    39661 taaggaattg gctcagaaac cttatattgc aggcaatgac tatacgattg cagatattgc
    39721 tatctggtct tggtatggac agttagttca aggaaatctt taccaaggtt ctgcaaaatt
    39781 cttggatgcc tcaagttatc aaaatctagt aaaatgggca gaaaaaattg ccaatcgtcc
    39841 agctgttaag cgtggcttgg aagtaactta tacagaaatt aaatagcaaa aaagtcatct
    39901 tcggatggct ttttgctaca gttgagggaa agggtttgca gttatcggct tttgtgctat
    39961 aattgctata atatagtaaa aagcaagagg agtgtgagga agtggaaaag aaaattgtga
    40021 aggatatctt atttttatct caagtgtctc atccggcaag tcaggaggac ctttatcttg
    40081 ccagagattt gcaggataca ctcttagcaa atcgtgatac ctgtgttggt ctagctgcca
    40141 atatgattgg gatgcagaag cgcgtgatta tctttaatct tggcttggtt cccgtggtca
    40201 tgtttaaccc agtgcttctg tcctttgaag gatcctatga ggcagaagaa ggctgtttgt
    40261 ccttggtagg tgtgagatca actaagcgtt atgaaaccat aaggcttgcc tatcgtgaca
    40321 gcaagtggca ggaacagacc attaccttga caggcttccc agctcagatt tgccagcatg
    40381 agctggatca cttggaagga cgaatcattt aggaggaaag caaatgaaac gaatagtctt
    40441 tgaacttatt tttatcgcaa cgacctggta tatcttttta ccgcccctta acctgaccag
    40501 ctgggaattt ctcttcttcc tctgtgggca tttgttagtt gtggcaatct tatttggctt
    40561 tggcaagggg ataaaccttg tcaaaacggt tcatgtgcgc cacggtaagg cggaagctgc
    40621 cttaaatctt gagggtttca aaatcaatcg gttagggaaa attctgttag cttcgattgg
    40681 aggaattctt ctcttggcag ctttggtttc cttggtaact tctagcatgt ttcaggctaa
    40741 aaattatgcc aatgtagtca cggttacgga aaaagacttt actgaatttc ctaagagtga
    40801 caccagtaag gttcctatcc tagatagaag tactgctgaa aaaattggag accgctactt
    40861 gggttcccta accgataagg tgtcgcaata cgtagcggca gatacctata cccaattgac
    40921 aattgatggg aaaccttatc gggtcacacc actagaatat gcagacccta tcaaatggtt
    40981 taacaatcaa gccaagggaa tcggtgagta tatcaaggtg gacatggtaa ctggaaatgc
    41041 ggatttggtg gacttgaaga caccaatcaa gtattcagac tcggagtatt ttaaccgtga
    41101 tgtcaaacgt cacctgcgct tgaagtaccc gaccaaaatc tttaaaactc catcttttga
    41161 ggtggacgat gagggcaatc ctttctatgt agcaacggtt taccaaaagc aatttggact
    41221 tgctgttcct cgtcctgctt cagtcattat cttggatgct acaaatggag aaaccaagga
    41281 atacagctta tcagatgttc cagaatgggt ggacaggatc tatccagcag aggaaaccat
    41341 tgagcaaatc aactacaacg gcaagtacaa ggacggtttc ttgaatgcca tgatttccaa
    41401 gaaaaacgtg acccagacga ccaatggcta taattacttg tctatcggta atgacatcta
    41461 tctctacaca ggtgtgacgt cggctaatgc ggatgagagt aatcttggtt tcatccttga
    41521 aaatatgcga acaggagaaa ttactaagta tagcttggct tctgcgacag aagaatcagc
    41581 ccgtgaatca gcagaaggtg ctgttcagga gaaatcctac aaagcaacct tcccaatcct
    41641 catcaacctc aatgacaagc ctctctacat catgggctta aaggacaatg ctggcttggt
    41701 caaagagtac gccctggtag acgcagtcga gtaccaaaat gttatcgttg ctactacagt
    41761 ggaagagatg ctcagcaagt atgccaataa aaacgacctt gaaattgaca atgcaacgac
    41821 agaaagcatc aagggagtag tagcagacct caaatcagct gttatcaagg gagacactgt
    41881 ctacttcttt aaagttgatg gcaagatata caaggtaaag gcttcagtat ccgatgacct
    41941 tccttacctt gaaaatggta aaaccttcga aggtcaagta ggaaaagaca attatctcaa
    42001 gacctttaaa gtacaataat actcttcgaa aatctctagc tctatctgca acctcaaaac
    42061 agtgttttga gcagcctgcg gctagtttcc tagtttgctc tttgattttc attgagtata
    42121 aaaaaggttt atttaataaa tatatgttat aataaggtaa attaagccta atggaggaca
    42181 aagaaatggg tttttatttg atggttgctt caatgctact cggactgctc gctttgaaga
    42241 taggtttttc tcagttcaag gagaaaaaag ataaattctt atctatctta acaagtttag
    42301 ctggcacagc ccttgttctc gtggctgtct ggttaggatg gcccaaataa atagaaaaac
    42361 ctcggtcaaa ccgaggtttt tcaaatgaat ttcttggttg tggctaaaaa atatgctaca
    42421 ctatcaatat gaaaatttta atcccaacag caaaagaaat gaacacagac ttgccaagta
    42481 tcgaggcaat tcctttaaaa ccagaaagtc agaccgtgct tgatgccttg gctctctatt
    42541 ctgccagtca attggagagt ttctacaagg tatcagctga gaaagcggcg gaagaatttc
    42601 aaaatatcca agctttgaaa aggcaaactg ctcaacacta tccagccttg aaactttttg
    42661 atgggcttat gtaccgcaac attaagagag ataagctgac cgaggcggaa caagattatc
    42721 ttgaaaatca tgtcttcatt acctcggctt tgtacggtgt tgttccagcc ttgtcaccca
    42781 tggctcctca ccgtttggat tttttgatga aattaaaagt cgctggtaag actttgaaga
    42841 gccattggaa ggcagtctat gatgaagctc tgaagaagga agaagtgatt ttctctctct
    42901 tgtcatcaga gtttgagact gtattttcta aggaaatcag agcaaagatg gtgaccttca
    42961 aattcatgga ggatagaggc ggtcagctga agattcactc aactatctcc aagaaagcgc
    43021 gcggggcctt tctaacagct ttaatagaaa atcaagtaca aactgtgggg gaagcacgtc
    43081 gcttgaactt tgctggattt gtttaccgag aagatttgtc acaaccacag ggattggttt
    43141 ttgtaaagga agtatagaaa tcagataaga gaaaagtcag cacgattatg ctgacttttt
    43201 tgataactta taaagtattg gacaaatgaa tctcatttga taaggtgtta agatgattaa
    43261 ccaaattcat gattgacaga tgatttcggc aaataaactt cttggtaatc cctgaaatat
    43321 agaaattaga cacgatgata tcgtaatctg tctgatttaa aatctcagga cttgttttta
    43381 attcatccca aatatcgaag gtaaagcggt tattacagta ataggaaagc atatcaacaa
    43441 aggtaaggga tatagcatgg tcaaagttac tgataatcaa aaccttgata ggtggtcgat
    43501 tttctaacaa ctgagtagag aggttttcag catgggttaa gatggtgtac atcaagtgtt
    43561 ccaactgttc aggatggtca tgctgtccaa ttgcctgacg gtattgagcc agttcttgac
    43621 gcgcacttcc cataaagtct gggaagtaac cttcaaattt tttaatcgtc aatgcttttt
    43681 gttcaaataa gattggggta gaaaagattt cctgtctttc aaagaaagca gtgttgtgta
    43741 aatgccaaat cagctcatcg tggttagtaa aagtaatctt atattgtttt gataggcgtt
    43801 ctaatatttg actaagtaat tggtaagagt atcttgcttg actgttatct tctaaagagt
    43861 tgaagaattc ttgaggatct aggaaaatat cattctgaag aaaagaaata aaaatttgag
    43921 cgattgtatc tggagtcatt tcaagcccaa aatgtttaga gaaataagca agtgtctcct
    43981 ggaaatttgg aatgctcttt aaaagaggat aatattcttt gtaaaagtgg ttggggaggt
    44041 caatgaagtg accattttta accctgtgga tactgatagc tatcatcagt ttgtacattc
    44101 tataaattga aaatcgcatt ggataattcg tgattttata gaagaagtca gcaaactctg
    44161 ttaaatcttc ctcaggaagg tcaggaaaag gccaatctag gaaataatag cgctcagaaa
    44221 agtattgggc aaaaaagtaa cgaatatcga tctcatttcc acgtatctcg gatggagtaa
    44281 aagataattc aatttgaaac tgagatgaaa gaacctttgt aatatttctg cccagtcgat
    44341 agaggttggc actactaaag taaagatttt ctattgttcg ataaataggt agcccctcat
    44401 tgaagaacat atactctagt aattgaaaag attgagaatt agcaagaaaa tattgataaa
    44461 tatcttctac actagtgtta acttctaaat ctatcatgat tccgttaaca gaagattgga
    44521 tgttaataga tggaaaggca atacgtaatt catttaaatc actttttaag attcgttctg
    44581 tacatcctaa tttttcggct agttcatgta atttcatcca attacgattc tgaattaggg
    44641 tttccattaa gcgtaattgt ctttgaactt ttgtggataa aaggtttctc atacgggtat
    44701 ttcctccata attcattata tctttttttg tctaagtagt ataaaaaaat tagagcaaga
    44761 aaattgtcgt tagtaatcgg aaaaattaga ataatataag tggcttttaa gtgcaaaaac
    44821 agaatatatt aattgttcag aaaatattga ttaatgcctc aaatagttgt acattgtaaa
    44881 ctataaaata ctatctagga gtttaaaatg aaacattcac ataaaaaatc atttgactgg
    44941 tatagcatgc aacaacgtta ttctattcgt aagtattact ttggtgcagt tagtgtcttg
    45001 ctcggtaccg ctttggtatt aggtgcagca gctagtgtcc aaacagtaca agcggaagaa
    45061 aacaaacaag aaactaccaa tagtatttct gttggtaggg gagaagcagc tactaaacca
    45121 gcagaggttt ctgcgtctaa taaagagaaa acctatgcag ctccaactgt agctaatcca
    45181 gtagaaacga ctccagttaa aactggagaa gttactaaac cagcagaaaa agttgaagaa
    45241 gcaaaagaca aaaaagagga agtaacgcat caagatgcta ttgacaagtc aaaattgtta
    45301 acggctcttt cgcgtgctaa aaagttagaa agcaagttat atacagaagc aagtgctgct
    45361 aacttgcaaa caagtatcca agctggtcaa agcttgcttg ggaaagcaga tgcatctgaa
    45421 gctgaattat cagcagcaga gtcatctatt caatcatcta ttattggtct agaacttcgt
    45481 tctaactcta acaaaggaac tgtatcagaa actcctgtag cgaagaaagc taatatagtt
    45541 gaagcgaagg aagagactaa tccagctgca acaactgatc gttcagctgt tgatagtgct
    45601 gttttgccaa ctagcacagc tgccaaagta gaaacaactt cagctccagc atctattaat
    45661 gaaatcttga aaccaggttt gagcctttct gatgctcgcc aaaatccagc tatccgtaag
    45721 gaagatgttg atagagggta tagtggtttt agagcggcgc ctgctccaac gaaccaacca
    45781 gtaactaatc taggaaataa tactgttttc actgatatta gcagaggttc tcatactttc
    45841 cgtggtaatg gaaattcacg tcgtggaaac ccgattcatt ttgatgtaac aacaactaga
    45901 gttagaaata gagtagattt ctcaatcaga tattcaggac caggtgagtt tgttaataac
    45961 aactttatct tagacaaagg aaatggattc ggagaacctt ctcgtgcaac tgtttcaaca
    46021 ccaaaattaa gagaccaagt taaaaacatt agtaagggtg gaaactttgt ttctcattca
    46081 ggctacagtt tagaagctgc agtagaaaca agcaccaatc aaacaattaa gtttagttta
    46141 cctatagcca accctaatgg tgatctgtct gttcgattaa gacctgttac ttttaatgta
    46201 ggtcaagggg aatcaggatc tgcaactagt aatgatccat atagtaactc taactattac
    46261 ttcagagaaa aaccactcct cttggatgca aatccaaatg gtggtactaa taataagact
    46321 gtttcagaag acattgactt ccaaactgtc tatcttccaa ctagtaagtt accagaaggt
    46381 caaactagat tagttcgaga aggtgaaaaa ggacaacgtc aaatcatcta taaagttcat
    46441 caattgggta acgaaacaat cctaggattg ccaattagta attcagttat taaagaagtt
    46501 aagccacgta ttatgcaaat tggtgtggct aaagatctaa tcgatacagt aaaaccacgt
    46561 gttgatcaaa ataaagtcgg tgatacaaat aacctcactt tctatcttga taacgatgga
    46621 aacggtgttt atactgaggg cgtagacgaa cttgttcaaa aaattgctat taaagatggt
    46681 gctaaaggtg aaaaaggaga ccgaggcgaa cgcggtctaa ctggagctca aggagctaaa
    46741 ggtgaaaaag gagaccgagg tgaacgcggt ctaactggag ctcaaggagc taaaggtgaa
    46801 aaaggagacc gaggtgaa
//
LOCUS       DAVBCV010000008        45795 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBCV010000008 DAVBCV010000000
VERSION     DAVBCV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.008"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(1..143)
                     /locus_tag="VQ516_000460"
                     /pseudo
     CDS             complement(1..143)
                     /locus_tag="VQ516_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(205..654)
                     /locus_tag="VQ516_000461"
     CDS             complement(205..654)
                     /locus_tag="VQ516_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2510959.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(656..1414)
                     /locus_tag="VQ516_000462"
     CDS             complement(656..1414)
                     /locus_tag="VQ516_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2510960.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1529..3508)
                     /gene="pknB"
                     /locus_tag="VQ516_000463"
     CDS             complement(1529..3508)
                     /gene="pknB"
                     /locus_tag="VQ516_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2510961.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3505..4245)
                     /locus_tag="VQ516_000464"
     CDS             complement(3505..4245)
                     /locus_tag="VQ516_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2510962.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVFMNEEDAE"
     gene            complement(4260..5573)
                     /gene="rsmB"
                     /locus_tag="VQ516_000465"
     CDS             complement(4260..5573)
                     /gene="rsmB"
                     /locus_tag="VQ516_000465"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2510963.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5566..6501)
                     /locus_tag="VQ516_000466"
     CDS             complement(5566..6501)
                     /locus_tag="VQ516_000466"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2510964.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6514..8910)
                     /locus_tag="VQ516_000467"
     CDS             complement(6514..8910)
                     /locus_tag="VQ516_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2510965.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGTVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8976..9290)
                     /locus_tag="VQ516_000468"
     CDS             complement(8976..9290)
                     /locus_tag="VQ516_000468"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2510966.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9315..9941)
                     /gene="gmk"
                     /locus_tag="VQ516_000469"
     CDS             complement(9315..9941)
                     /gene="gmk"
                     /locus_tag="VQ516_000469"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2510967.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10071..11684)
                     /locus_tag="VQ516_000470"
     CDS             complement(10071..11684)
                     /locus_tag="VQ516_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2510968.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11875..12129)
                     /locus_tag="VQ516_000471"
                     /pseudo
     CDS             complement(11875..12129)
                     /locus_tag="VQ516_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(12133..12387)
                     /locus_tag="VQ516_000472"
     CDS             complement(12133..12387)
                     /locus_tag="VQ516_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET2510969.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12625..13722)
                     /locus_tag="VQ516_000473"
     CDS             complement(12625..13722)
                     /locus_tag="VQ516_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2510970.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKVRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13732..14472)
                     /locus_tag="VQ516_000474"
     CDS             complement(13732..14472)
                     /locus_tag="VQ516_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2510971.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14792..15145)
                     /gene="rsfS"
                     /locus_tag="VQ516_000475"
     CDS             complement(14792..15145)
                     /gene="rsfS"
                     /locus_tag="VQ516_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2510972.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(15158..15658)
                     /locus_tag="VQ516_000476"
     CDS             complement(15158..15658)
                     /locus_tag="VQ516_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2510973.1"
                     /translation="MKTAFIIIDVQNILVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DCKHIF"
     gene            complement(15659..16252)
                     /gene="yqeK"
                     /locus_tag="VQ516_000477"
     CDS             complement(15659..16252)
                     /gene="yqeK"
                     /locus_tag="VQ516_000477"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2510974.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(16252..16881)
                     /locus_tag="VQ516_000478"
     CDS             complement(16252..16881)
                     /locus_tag="VQ516_000478"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2510975.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16933..17244)
                     /gene="yhbY"
                     /locus_tag="VQ516_000479"
     CDS             complement(16933..17244)
                     /gene="yhbY"
                     /locus_tag="VQ516_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2510976.1"
                     /translation="MSLASKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(17458..18564)
                     /gene="yqeH"
                     /locus_tag="VQ516_000480"
     CDS             complement(17458..18564)
                     /gene="yqeH"
                     /locus_tag="VQ516_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2510977.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVATRKAII"
     gene            complement(18567..19157)
                     /locus_tag="VQ516_000481"
     CDS             complement(18567..19157)
                     /locus_tag="VQ516_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2510978.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(19109..20017)
                     /locus_tag="VQ516_000482"
     CDS             complement(19109..20017)
                     /locus_tag="VQ516_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2510979.1"
                     /translation="MVLEKQLGHGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(20074..20634)
                     /locus_tag="VQ516_000483"
     CDS             complement(20074..20634)
                     /locus_tag="VQ516_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2510980.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20773..21982
                     /gene="sstT"
                     /locus_tag="VQ516_000484"
                     /pseudo
     CDS             20773..21982
                     /gene="sstT"
                     /locus_tag="VQ516_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            22050..23003
                     /locus_tag="VQ516_000485"
     CDS             22050..23003
                     /locus_tag="VQ516_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2510981.1"
                     /translation="MSISQRTTKLILATCLACLFAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(23239..23319)
                     /locus_tag="VQ516_000486"
                     /pseudo
     CDS             complement(23239..23319)
                     /locus_tag="VQ516_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(23374..23598)
                     /locus_tag="VQ516_000487"
     CDS             complement(23374..23598)
                     /locus_tag="VQ516_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HET2510982.1"
                     /translation="MIEMLIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(23603..23782)
                     /gene="asp4"
                     /locus_tag="VQ516_000488"
     CDS             complement(23603..23782)
                     /gene="asp4"
                     /locus_tag="VQ516_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HET2510983.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(23784..25121)
                     /gene="gtfB"
                     /locus_tag="VQ516_000489"
     CDS             complement(23784..25121)
                     /gene="gtfB"
                     /locus_tag="VQ516_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HET2510984.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPEH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQEIMQTVLGG"
     gene            complement(25114..26625)
                     /gene="gtfA"
                     /locus_tag="VQ516_000490"
     CDS             complement(25114..26625)
                     /gene="gtfA"
                     /locus_tag="VQ516_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HET2510985.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(26637..29009)
                     /gene="secA2"
                     /locus_tag="VQ516_000491"
     CDS             complement(26637..29009)
                     /gene="secA2"
                     /locus_tag="VQ516_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET2510986.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSSLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(29002..29442)
                     /gene="asp3"
                     /locus_tag="VQ516_000492"
     CDS             complement(29002..29442)
                     /gene="asp3"
                     /locus_tag="VQ516_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HET2510987.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(29439..30974)
                     /gene="asp2"
                     /locus_tag="VQ516_000493"
     CDS             complement(29439..30974)
                     /gene="asp2"
                     /locus_tag="VQ516_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HET2510988.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(30958..32538)
                     /gene="asp1"
                     /locus_tag="VQ516_000494"
     CDS             complement(30958..32538)
                     /gene="asp1"
                     /locus_tag="VQ516_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HET2510989.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(32548..33765)
                     /gene="secY2"
                     /locus_tag="VQ516_000495"
     CDS             complement(32548..33765)
                     /gene="secY2"
                     /locus_tag="VQ516_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HET2510990.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYFYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(33822..34499)
                     /locus_tag="VQ516_000496"
     CDS             complement(33822..34499)
                     /locus_tag="VQ516_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2510991.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(34484..34726)
                     /locus_tag="VQ516_000497"
     CDS             complement(34484..34726)
                     /locus_tag="VQ516_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2510992.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGQHKLKIWGLV"
     gene            complement(34726..35922)
                     /locus_tag="VQ516_000498"
     CDS             complement(34726..35922)
                     /locus_tag="VQ516_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET2510993.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(35931..37151)
                     /locus_tag="VQ516_000499"
     CDS             complement(35931..37151)
                     /locus_tag="VQ516_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET2510994.1"
                     /translation="MRNTKRAVAFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(37182..39626)
                     /locus_tag="VQ516_000500"
     CDS             complement(37182..39626)
                     /locus_tag="VQ516_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HET2510995.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYTRYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELDKEFNLQVGHDVTAFYNKWENYFNE
                     LVPPSIIHFVSYRKPWTTLIANRYRDLWWEFHDLEWTKILQHHIGEFELTSSLDKEFS
                     CLTLTNSQDLEGIEELVTALPDVVFHIAAWTDMGDKLIKLAVYDNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHEIEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(39636..40646)
                     /locus_tag="VQ516_000501"
     CDS             complement(39636..40646)
                     /locus_tag="VQ516_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2510996.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTYQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFVANSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(40675..41550)
                     /locus_tag="VQ516_000502"
     CDS             complement(40675..41550)
                     /locus_tag="VQ516_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2510997.1"
                     /translation="MENELISIVVPIYNVENYLRQCLDSIQKQTYPNFECIMVNDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(41590..42468)
                     /locus_tag="VQ516_000503"
     CDS             complement(41590..42468)
                     /locus_tag="VQ516_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HET2510998.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            complement(42465..44550)
                     /locus_tag="VQ516_000504"
                     /pseudo
     CDS             complement(42465..44550)
                     /locus_tag="VQ516_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(44611..>45795)
                     /locus_tag="VQ516_000505"
     CDS             complement(44611..>45795)
                     /locus_tag="VQ516_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2510999.1"
                     /translation="ASTSASESASTSASASASTSASASASTSASASASTSASASASTS
                     ASASASTSASASASTSASTSTSTSASTSASTSASTSASTSASTSASESASTSASTSAS
                     TSASASASISASESASTSASASASTSASASASTSASESASTSASASASTSTSASESAS
                     TSASASASTSASASASTSASASASTSASESASTSTSASESASTSASASASTSASASAS
                     TSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTS
                     ASASASTSASASASTSASASASTSASASASTSASESASTSASESASTSASASASTSVS
                     NSANHSNSQVGNASGSTGKSQKELPNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDE
                     EE"
BASE COUNT        13348 a        10285 c         8222 g        13940 t
ORIGIN      
        1 cagaactgag aggagttctt gaaatcgtaa caccaccttt gaacaagagt tacttcaacc
       61 cattggctcc gacggagtaa gttgctttcg tggataccaa aatcagccgc aatttcttca
      121 taagtgcggt attctcgcac atatagaaag cgttgttaat ttatttatct catttttcag
      181 aaaattcttt tatttctgta aagtctacga tactcgatgt gtttttatat aatgatacag
      241 tctgagaatc actgttccgc tagccattcc aatagagatt accaaagcca acatgacaac
      301 caaggtcgca cttgccagtg ctttattata gtcccctgtc acaaaaaagg cagttgttcg
      361 gtaggagaga taacctggaa ccagcggtgc caaaatggcc aagataaaga ccacagcagg
      421 tgtcttataa agaatactta aaatctggct gacacaagaa ccaataatgg ctgcaatgaa
      481 ggtagctaca atgacattgg tcggttcctt gagcaagaga tagattagcc agacagtcat
      541 gcccaaaatc cctccaggta agagcataga ccgttgcaca ttgagtacga ttaaaaaagt
      601 gataatggca agaaaacttg ctactgcttg taataaaaag gttgttagtg tcatattagt
      661 tcatcaatac caaggcgaca gaagttcctg cccctaaagc gagggtaatg agcagggatt
      721 caaacatctt actcatacca gagtttatgt ggttggtcat aatatcacgg accgcattgg
      781 tcaaggcaat acctggtaca aacggcatga ccgcaccagc tataatcaaa tctgccgttg
      841 aaggaaaacc tgtgtagcga gcccaaaact gggcaattat cccaaagaca aaggctccag
      901 caaaggctgt cacaaaggga attcggataa atttttccac atagagggaa aaggcaaaac
      961 caaataaggt cgccactcct gccccaagtg cgtcgtagat atttccgcta aacataactg
     1021 aaaagaaagg agcactaaag gtcgcagcca gagttacctg caacttagta tagggaaggg
     1081 gttgagcttg caaggccgtc aattgcttaa aggctgtttc taagtcaatc tgccccccaa
     1141 ctagctgacg agaaatctgg ttcacatcgc agactttttc gatgttataa gaagaggagg
     1201 tcacgcgctt catgcgcgaa atattggtat tttcaataga gaaaaagata gcggcaggca
     1261 tggcaaggac attgcaatcc acaatcccct gcgaatgcgc gattcgaatc atggtatctt
     1321 ctacacgatg gatttctgag ccacttttaa ggagaatagt ccccgctagc ataatcacat
     1381 caatgacggc atttaattct tttgattctt ccatgctttc ctccttttat caactccctc
     1441 tattctatca caaatccgga ctcaaaaaaa atctttgcca tgaaatcatg acaaagattg
     1501 attactcatt ttgattatcc atctgctttt aaggagtagc tgaagttgtt ttaggtttgt
     1561 agattgaaat cttgactcta gtcttattga ggtctacctt ttcacctgct ctaggacttt
     1621 gttcaacaac catgccttct acactacctg caggcgctgt cgtcacttct acaacttcta
     1681 tattagcttc cttaatccca acaatttgaa tcaaattgtt cttagtaaac tccaagctgg
     1741 aaccaatgta actcggcatg gcaacacttg taactttttt agctactgtc aagacaattt
     1801 gagtaggttt actcacatca taagtcgttc cggcacctgg actttgtttc ataatcgttc
     1861 ctggttcgct ttcgctggac tcttcttcct ctatcttaat caaattctca ggaaccttct
     1921 tctgcttgag ttctgagatt acttctgtag agttccgtcc aatatagttc cctaattgaa
     1981 tcgtcgtagc ttttttagct actgtcaaaa caatttgagt tgccttgctc aagtcatagg
     2041 tcgtaccttc tggtagactt tgcttcagga ccgttccagc ctcactctca ttcgactctt
     2101 cttcctcaat tttaatcaaa ttatctggaa cttttttctc ttttaattcc gcgataacat
     2161 cagaagattt ccggccgaca taattactaa tttggaagga ttgtttgcct gatgagacaa
     2221 ccagattgat tttcgttcct tcttttcgac cagttccagc gccaggatct gtacggataa
     2281 tccgcccttc ttccaccttt tcactagcct ctgtcttctc ctcaccaatc tcaaaattgg
     2341 cttttttgag cgttgccttg gcctctgcaa ctgtctgacc tgccacatct ggaatggcaa
     2401 tggttgcagg agttctggat agtatccaaa taagagaagc tgccaccaat acaaggctgg
     2461 ccaacaaaat caggtaacgc atcttaaatc tatgtttttt cggtgcttgt ggttggtaag
     2521 tttcctctgt cacagcctgg cttgggtttt tgattgattt gtgttctgtc tgcgcttgaa
     2581 ccttaggaat agatgtcaag gtactctgag aaaccttcgg caaggtcttg gtatctgcct
     2641 tgctcgtttc atcaaagatt aacttacttt catttctacg attgtaggac aagctactag
     2701 acaagtccac atacatctct gaaaccgagc ggtagcgatt ggtcaacttt ttagcagttg
     2761 ccttgataat aacattttct aaagcctgag gtacagatgg attttctgca ataacggacg
     2821 gcaggggttt ctggaaatgc tggagggcaa tggtcaccgc gctatccccg tcataaggga
     2881 tatggcctgt caacatctca tagaaaataa tccccatggc atagatatca ctctgcacag
     2941 tcgccttcga accacgcgcc tgctctggtg acaagtaatg aactgagccc aacatcgagt
     3001 tagtctgggt cagacttgtc tctgcaaagg ctacagcaat cccaaagtct gtgaccttgg
     3061 cagtcccatc tggtgtcaaa aggatatttt gaggtttcaa gtccctgtga acaattcctc
     3121 gagtatgggc caagcgcata gccaagagaa tttgtcccat gatacggact gcttcttcat
     3181 tagaaagagg ataatgttcc ttgatatagc gtttgaggtc cagtccagcc acatactcca
     3241 tagctaggta ctgttgaccg tcttcctcgc caatatctgt tatccgaacg atatgaggat
     3301 ggtctagatc tgccatagct ctcgcttcac gctgaaaacg agctacagct atcgggtccg
     3361 tctggtagtt ggtcctcaga accttcactg ccacttcttc cccatctaag attaagtctt
     3421 tggctaggta gacatccgcc atgcctcctc gaccaatctg tttgacaatc cgatagcgtc
     3481 cggcaaaaat cttgccgatt tggatcattc tgcatcctcc tcgttcataa aaacaagggc
     3541 aaccgtaatg ttgtctaaac ctcctgcatt gttagcaaaa cgaacaagtg tctccgtttt
     3601 atctgctaaa ggaatatcac tggttacaat atcacgaatc tcactgcctg aaatcatgtt
     3661 ggtcaagccg tcactattga gcaagagata gtcacctgac tcaaggataa ctgtcccaaa
     3721 atcaggctga atttcatctt tttgcccaat agactgggtg ataatatttt tttgcggatg
     3781 agcttctgcc tcttctggtg tcaattgacc agccttgagc aattcattaa ccaaggaatg
     3841 atcgctcgtc aactgatggt attcttctcc acgaatcaag ccgatacgcg aatcaccaat
     3901 atgagcatag atagcctgat tatcaataat agcaaggact tccaaagtag ttcccatgcc
     3961 tctgtaagct tcatcctgac caagctggtg aatcttttga ttttcaattt ctaggtaatg
     4021 ggcgaaccat tcacgcactt cattgactgt atcgatctgg gtatcaaccc aagctacacc
     4081 caggtctgtg accgccattt cactagcgat attccctgca cgatgacctc ccatcccatc
     4141 agctaaaata atcatggtac gtccagctct attgacatag tggttgacat agtcttggtt
     4201 atttgttcgt ttctgaccaa catctgttaa taatgaaatt tccatgtgtc agttccttcc
     4261 taatccgata tcttgcgaaa ttgactgatg aagaatccat cacttccata caattcaggt
     4321 gtaatgagga tacagccgtc tttcatgata tccttacatt catgttctag ttttacctgc
     4381 tcgaactcgg gatgactctc taaaaaggcc ttaacgactt gaaaattctc ctctgagacg
     4441 atagtgcagg tgctataagt tattatacca cctttgccta gtatttgaca aacactacct
     4501 aatatttcta actgaatttc ctgcaaggac gcgaaatctg ccgtttcttt attgtatttg
     4561 atatctggtt ttcggcgcaa aagaccgatt cctgaacaag gagcatccac caaaatctta
     4621 tcaaaggaat cctggtcaaa aaactcatgc acctttctgg catccaattt ttgagtttga
     4681 acccgatctt caactcccag acgttgggca ttttcttgaa ttaaatccaa cttgtggtcg
     4741 tacaagtcca gagcagtaac ctgacctgtc gtaagataag aggctatatg ggctgttttc
     4801 ccacctggag ccgcacaggc atcaagcact cgctcatcac cttgtaaatc aagcgtcgga
     4861 gcaaccaact gactggactc gtcttggatg gtaatggctc catccgcaaa caaattatgc
     4921 cctgcaaaat gcccctgctc cttaaccaga ccagtggttg ctaaaaggga attattcgcc
     4981 tccaacaagg cttggatttc ctcttttcga cttaggtctg ttacacgaat actggctttg
     5041 tttcgcacta acaggctttc aaagatggct tttgctctct cctctccgta ttcttccttg
     5101 agtttggcaa ctagccaaac tgggagagaa taggcaatgg agtcacgctt gttttttcgc
     5161 ttgatgctag caatatctgg ccagccttca cgcaagatac ggcgaaggac agcgttgacc
     5221 aatttttcac tgcctttttt acggagtttg gccaattcca ctgcttcatt gaccacagca
     5281 tgatctggaa tcttgtccaa atagcggagt tggtaggcac tcatgagaag aaggacatag
     5341 agccagctgt ctaactggtc tctgtcttcg ataaagtggg ataggtacca ttccagagtc
     5401 agtttacggg ctaccgttcc atagactagc tcggtcacta agcccttgtc tgctgcagag
     5461 agctgactcc ccttgaggtg tttatttaag gcaatatttg aatatgcttg attcacaaaa
     5521 acatcctcta gcactgctag agctaaactt ctagccgttt ctactttagt caccaaatcg
     5581 ttctcctaca gtcaatgtac gtccaactcc attgaggaag gaagcaatgt ccatcttagg
     5641 cttaccagct ggctgcactt gtttgaggga tagagcccct tcagccgttg cgacaatcaa
     5701 ttctttcttg ccgatagaga gaatctcacc tggatttccc tgaccttcta ctggtagggc
     5761 ttcataaatc ttaaagcggt cgcccttaag gaaagtatgg gcaacaggcc aggggttcat
     5821 tccacgaatt tggttaaaga gttgacgatt ggttttgttc cagtccagtt tttcttcctc
     5881 tggctttata tttggagaga aggtaacctg actcgtatcc tgcggttcag gtttgatatc
     5941 accagcaata taggcaggca gagtgtccaa aagcaaatca cgaccaacta gcgccaattt
     6001 ttcaaacaag gtgccaacat tgtcctcatc tgtgatcgga atgctgcgac gagaaatcat
     6061 atctcctgca tccatttcct taaccatttc catgatggtc acaccagctt cctcatcccc
     6121 ttgaatcaag gcataatgga taggcgcacc accacggtgt ctaggaagga gggaggcatg
     6181 aacgttgaca gcaaagtcca tgctatcaag gagtttgctt gggagaaact gcccaaaagc
     6241 agcagtcaca attccatctg ctcctagctt cataagttct tccatctctg gacttccaga
     6301 taatttttca ggttggtaga tagatagtcc tgcttccttg gcagcctgct tgactggggt
     6361 ttcttggata acttttttac gaccaacagc acggtctggc tgggtcacaa cggctagaat
     6421 ttcgtaacgg tcatctgtca aaagtccttt taagactgtt gctgaaaagt cgggggtccc
     6481 cataaagatt agttttgtca tatcttctcc ttcttataaa aattgctgcg gctcatggtc
     6541 aatgctgaga cggagctcac tattttcccg ttcttgagtc aaggccaaga cctggttgag
     6601 ggtcggcccc agctcatctt ctaaacggta tttaattaaa atctggtaat gatagaggtt
     6661 gtgggtacgg gcaatcggtt ttggcgttgg ccccagaatg ggactggtct ctgacaagcc
     6721 tgaccgcaaa atgttcatga cttcataggc acgtttgaaa acctcttctt ctttcttgtg
     6781 agaaagggta ataccaatcg tgaaatagta aggtggatag ccgagttgtc gtctgattcc
     6841 catttcatag gcataaaagc cttcgtaatc ttgatccttg gcaaatcgaa tagcatagtg
     6901 ctgcgaattg taggactgta tcaagacttg acctgctttt tcagcccgac ctgctcgacc
     6961 tgccacctga gtcaagagct ggaaggttct ctcagaagaa cggaaatcag gcagattcaa
     7021 ggccgtatcc gcatttagaa ctccgactag ggtaacattg ggaaaatcca aaccctttgc
     7081 aatcatctga gtaccaagta aaatatccgc ttcccctcgc ccaaactggt caagcaaggc
     7141 ttggtgactg cctttctttc gagtcgtatc cacatccatc ctcaaaatgc gagcttgggg
     7201 aaagagttct gctagctcat cataagcctt ctgagttccc gtcccatagt aacgaatact
     7261 gcggctctta cagttaggac agacctgagg aatatccttc gagaaaccac aataatggca
     7321 gttcatagtc ttggtatcca tatgcaaggt cagagaaata tcgcagttgg gacaagtatc
     7381 caccgtccca cactcccgac acatgacaaa gctagaataa ccacggcgat tgagcatgag
     7441 aaccacctgc tcttttttaa ccagacggtc ttggatagcc tctagcaaag gaggcgtaaa
     7501 gtttgacgtc tcattttgtc cgatatagtc tcgaaagtca atcacttgaa cctcagggat
     7561 tgtagccaaa ggattggcac gttgggttag acgtaagtgt tgatagacgc ctttgccagc
     7621 acgtgcccgg ctctctaagc tcggcgttgc agatccaagt accagagttg cttgattata
     7681 ctgagcccgt aaaatagcta cctctctggc atggtaacgg ggattgctgt cctgcttata
     7741 agccgcttca tgctcttcat caataatcat gacacccaga tttttcagag gagcaaagat
     7801 agcagatctg gcaccaacaa caacttgggc atcgccacgc tccaccttgc gccattcatc
     7861 atacttttcg ccattggata atcctgagtg aagaatggct accttgtccc caaaacgtgc
     7921 tataaaacgc tcggtcatct gaggagtcaa ggaaatctca ggtaccagca aaatagctgt
     7981 cttaccctta tccagggcac cttggataat ctgcaagtaa acctcggtct tcccacttcc
     8041 tgtaatccct tgaagtagaa agggaggttg agaactgcca atagaactca caaccgcatc
     8101 acgcgcctgt ctttgttctg gatttaactc caaaggtcta cttgcttcaa ttccttcaaa
     8161 ataagcagcc gagcgttgaa cttccttttg gactatggta acagtacctt gatccacaaa
     8221 gaagttgact tgctctcgcg agtaggactc taacaagcta gccaaggaag cgctctctgg
     8281 atgagacagc agataatctc tcagttccaa ctttttcttg gcacgtgtag aaatctcaac
     8341 accttctaat tgggcatggt caacctcata ccaagactgg gtcttgacct tcttttgatc
     8401 gactgcctga tattccagac caagcaggcc ttttctagtc aaacgcatca tttcagcttg
     8461 cttggcaagg tctagtgaag aaaaggctag cgaatcttct gaaccaaaca ggcgtactcg
     8521 ttcttcctga ctcaagcctt ccagaggata gagaatcttg tcatagctag aattcagaaa
     8581 ccctggaagc atggccttga ggatagagat tttgtaggag aagacagatt tgcgtaactc
     8641 ctcagccagc cagagttgtt ctggcgtgag aacaggagaa aaatccagca cctctgcaat
     8701 atcttttaaa tcttgctcca tctcttctcc atctgattgg gacttcaaac caagaacaat
     8761 cccttgaatc aggcgattac ccttaccaaa aggcacatga acccgcatcc caacttccag
     8821 cattccctca aattcctccg gaatcctgta actatagggc tggtccgtct gcatcaaggg
     8881 cacatctacg ataatcttag ctagggccat cttctcacct cctccttgtc agtacattct
     8941 tgcaatagaa aaaataagat tgagtccccc caaccttaaa ttttttcacc atcttctttt
     9001 tctttagcaa tttgctcttt gattttcttt tcttcttctt ctttgcggcg tttttcttct
     9061 tcgatacggc gacgcactgc ttcacgtttt ccttctggat ctgggtgaat tgtaacgttt
     9121 cctgattcga tttcttctaa agcgcgaaga gttgattttt cagacttgaa accttgagtt
     9181 gctggggcac ctgcttccaa ttcgtgggca cgttttgctt ccaagattac gagtgaatat
     9241 tttgaaggaa ccttgtcgag caaggtatca atagagggtt ttaacatcat ttgcttgtac
     9301 ctattttcta aattttatcg ggtagttgga gattttggta acatctcctg atagtgacca
     9361 atgacacgat ccacacagaa gtgttctgct tcaatcacac atttgacacg ttcagcagct
     9421 aggggcacct gatcgttgac aatcgcataa tcatactcac gcatgagggc aatttcttcc
     9481 ttggcctttt cgattcgttg ggcaatcact tctgcactat ctgttccacg acctaccaag
     9541 cgatcttgca attcatccaa atctggtggt gtcaggaaga taaagacagc atctggaacc
     9601 tttttcttga cctgaagagc accctgaact tcaatttcaa ggaaaacatc gattcccttg
     9661 tccaaggttt cattgacata ggtcagagga gttccatagt agttgccgac atattctgcg
     9721 tattccaaca tctgtccttg acgaatcagc tcttcaaatt cttcacgagt acggaagaaa
     9781 tagtcaacac cgtccacttc tccaggacgt tgtgcgcgtg tcgtcatcga tacagaatat
     9841 tgaaattggt tttcagaact ctcaaaaatc tctcttctaa ccgttccttt tccaacccct
     9901 gaaggaccag aaaaaacgat tagtaagcct cggtctgcca ttgtgtctcc ttttagtcaa
     9961 tctgtgaaat aacatttctc tagaataatg gcaaaaagcc agattatcct ttacagtctt
    10021 tctatctagt gtaacaaaaa agcagtaatt tttcaactgc tctttcttat ttatttagca
    10081 taatctactg cacgaagctc gcgaatcacg gttaccttga tatttcctgg ataatcgaga
    10141 ttgttttcaa ttttcttacg aactttgtga gccaagattg tgactttgtc gtccttgatt
    10201 tttcctggat tgaccatgat acgaatttca cgtcctgctt gaagggcaaa gctagtttgc
    10261 actccttcaa agccgttagc aatttcttcc aaatcatgga gacgcttgat gtagctttca
    10321 agagactcac tacgagcacc tggacgggct gcgctcaagg catctgctgc agcgacgata
    10381 actgctatca cgctctcagc ttcaacatct ccgtggtgac tagcaatcgt attcaccaca
    10441 actgggggtt ccttgtactt acgggccaat tccataccga tttcaacgtg gctaccttca
    10501 acctcatggt caatggcttt cccgatatcg tgaaggaatc cagcacgacg ggcaagagcc
    10561 gcattttcac caagttcgct cgccatgata ccagccaact tagcaacctc aatcgaatgg
    10621 cgcaaaacat tttgtccata tgaagtacgg aactgcaaac gtcccataat cttcatcaag
    10681 tctggatgaa ggtttggcgc accaatttca taggcagcag cctcaccgta ttcacgaatc
    10741 ttattgtcaa tctcttgacg gtttttctca accaactctt cgatacgagc tggatgtata
    10801 cgaccatctt tgagcaacat ttccatagtc atacgggcaa tctcacgacg aatcggatca
    10861 aatcctgaca aggtcaccac ttctggtgta tcgtcgataa tcacatcgac ccctgtcaaa
    10921 ctttcaaagg tacgaatgtt acgaccttca cgaccaataa tgcgtccctt catagtatcg
    10981 tctggcagat gaactgttga gtttgttgac tccgctacat attcaccagc gatacgttgc
    11041 atagcttgaa ccaagatgtc cttggccatt ttgtcagaac gttccttgac ctcttgctca
    11101 gcttcgcgaa tgcgactggc aatctccctg gtcaagtttt cctctgtctg agccaagata
    11161 atatctcgtg cttctgcctg agacagagca ccaatacgct ctagttctgc ttctttttgt
    11221 ctttcgactt cctctaattg ctcttcacgc gcatcaaggt ttttcgctct atcagaaata
    11281 ctttgttctt tttgttcaag tgtttgttct ttactcgtca aattgtcgtc cttacggtca
    11341 aggctagtag ctctctctgt caaacgactt tcgatttgtt tgagttcttg acgttctgat
    11401 ttgaattcag cgtccacttc ttcacggtat tttctggctt cttctttggc ctccaatagt
    11461 gcttcttttt taagagactt gctttcacgt ttggcttcat taacaagtaa atccgcttca
    11521 cgctcagctt gtccacgtaa attagttgct tcttgttcag catttaaaag catcaactct
    11581 gcagcttcct gagatgattt catcttagct gagatgctga catatccaat gactaaacca
    11641 atgatgacgg caaaaacagc aatcgcaagc gacatgattt ccatgttttt acctcatttt
    11701 attgttattc cgaatgacat acattctttt acattctacc ataaaaaagt gattttcaca
    11761 aacctaaaat agaatatgtt ttggggaatt tggaacacat ttaccaaaat aaacttgttg
    11821 tttagaaata gtagtttagt agagacttga gaaaaagcct actttgcagt agacttagta
    11881 atgatcttta aaggacaaga aagccacgct atctccatcc atcatataaa tcaagcgatt
    11941 ttctgcatca atacgccgtg accaggctcc ttggtaatca tatttgagtg gctctggttt
    12001 tcctattcct gtaaatggat cgcgttgaat atccttgatt aatttattga ttctttttag
    12061 tgttttttta tcttggtttt gtcagtagca ataatctgcc caggcatctt ctgtaaactt
    12121 gagtagcatt catcactcct caatcacatg gacctgagtg cttccagcac ggacttgagc
    12181 cattcctcgc aaaaccttgt ctgataactc cttattttgg gcgattctca gggtttcctg
    12241 aatactgtcc cattcgctct ttgaaagaac tacaatgtcc tcatctggat ttttattgac
    12301 caccgtcaaa ggctcaaatt catcatttac cttcttcatg tagtccttta aatgatttcg
    12361 gaatgttgag taaaggactg cttccataac catacctcgt tttagctctt ttccactatt
    12421 atacacgaaa agaaagaaat tgtcaggaac ttgtacaaga ttttcttttc tatctattta
    12481 tactcaatga aaatcaaaga gcaaactagg aaactagccg caggctgtac ttgagtacgg
    12541 caaggcgacg ttgacgcgat ttgaatttga ttttcgaaga gtattattcg taaaaaatct
    12601 caaaaagcct acctttcggt agacttagtt tgtttctatt ctaatcggca ctcttccaaa
    12661 attttgctct gctatacttg gcttccctag ttggtaaatc tggtctgctt tttgggtcat
    12721 agcatcccaa ggttctttgc caattcggct gactagattg acctgtcctt tcagagactt
    12781 gagatgttgc ctgacttttt cagtaaatcc aaggacatga atggcttctg gcaagacatt
    12841 ttctctagcc tgtaccaaaa tataggtcaa taggcgtctg acacgcgcct tggtgtaacg
    12901 tttggtagtc actaactcga ccaattcttc cacagactgg gctgttttaa tagcttcttt
    12961 aatgcgcaca gccatttctt gattgacctg atagatggtg gttaggtcgg gatttgacaa
    13021 gatttgatag cggagcaagg gaaaatagtc ttcccagatc accttactgg cctgctcaaa
    13081 tagggcaaca gaaggcataa aacgttctaa gaaatcttgg tccttctgat gttgacggag
    13141 ggctgtcgcc gaggcaaagt ccacatcttt attcacagaa tggtaacctg ccccctgacg
    13201 ctgaatcgga tgcagcttga tatttcgtcc tgcaaccgcc ttggcatagg ccagagcaag
    13261 aacatgattg ggggtattac ctgaaaaatc aagaccagca aattccttcc acatagcttg
    13321 ggttttctgg ggataggaaa gggaatcagg cagatttttc acaaatttct ccatctcagc
    13381 acctttctct gtgtataagt cagcgatttt ctggtaatcc agaacttcct ctgtcccaaa
    13441 aactagacta tcaatgccca agcgatccaa gatatccaca gctccttgac caaagaaatc
    13501 agctgcctga acactgacta aaaagggcaa ttccactacc aagtctgcgc cattttccag
    13561 cgccatctgg gcccttgtcc acttatccac aatagcaggc tctccacgct gcatgaaatt
    13621 ccctgacatg gcaacaattt tcagtccctc tgcctgatcc agcaggtatt tatggccatt
    13681 atgaaaagga ttgaactccg cgataatacc tgttatggtc atgctaatct cctacttctg
    13741 cgccacaaaa aaccaacggg tgctagtttc tgttggctcc ttatcctcaa agtctgcata
    13801 gagtttgaag gacttaaatc cagcctgttc cagcaagata tcataggtca agatctcata
    13861 ggtcctctcc tcatgtactt catcgtggcg actaaaggaa ccgtcagcct ccttgataaa
    13921 gaaagtcagc tcatgcacga tggagtgagg aacttcgtcc tcatatgtat cccaaagcat
    13981 ggcaaaatct tccgcatttt catggtagga atagccaggg aatacttcat ctgtctggta
    14041 ggtcgagtgc acgtcaaaga tgaaaactcc ctcttcattc agcgcattgt acacgtcctt
    14101 aaagacgtcc cctacttcca cctcatcctg catatagcag atagagtccg aataacacgt
    14161 gacaaaatcg tatttccctg ccttggacaa attcagcata ttaccttcta taaaagcaat
    14221 cttttgcttg gctgaagttg ctctcttctc cgcaatcttc aacatatccg cactcaagtc
    14281 aagtccagtc acatcaaaac cagcctgaga aaagcgcact gattgaattc ctgtcccaca
    14341 agccaattcc aagagtttct ttctctcctt ggtcttaggc aaatgacgca gagaaaaatt
    14401 cgtccatttg tcgtataaac tatcgtccat gaccgcatca tagaccgccg caaaggtttc
    14461 ataagtcgcc ataaaacatg ataccaagtc cgcccgtaat tcgatagaaa attaggagcc
    14521 ttacgatgga gccgagctcc aaggaaggct atctattttc cgagaattac aggacgggtt
    14581 caaatccttt ctaagataca aagagcctta aaagcaaaga gtacatagga aactttcttc
    14641 cctatcgtca gatacaaaga aagttacttt ttatctcagc ttttagctcg tgttcagttt
    14701 cgaaatgaaa caaacacgag gccctttacc tttctatcaa tttttctaaa ataagcaaag
    14761 aaacccagtt gactacaact gagttcatct tctaagcaag agcttctgaa atatctactg
    14821 aattcgcctc atgccatagc ttctctagat tataatgggc acgcatttct tctgaaaaga
    14881 tatgcacgac aacagcacca aggtccagta agacccagcc tccagctgca tcgccttcga
    14941 catggctacc tttaaagcct gcttgagcta ctttttcacg gatattagct gcgatagcgt
    15001 ccaactgacg gctattcatt gagctagtga tgacaaagta gtccgtcaca ctagtcaaat
    15061 cttgtacatc aagtgcgagg atatcctccg cacgtttctc atcagccgct ttcacgacta
    15121 gttctagtaa ttctttttcg ttcatttaat cctcttttta gaaaatatgt ttgcaatcta
    15181 gaacatctgc aaagcgctcc tcccaaatgt cctcataaaa ttcattaatc gtttctgctg
    15241 gaatgtcatc cccatcaaag gttgtgacag caccttctgg aacaataagc tgatagccat
    15301 attcaaaggc aaccttgaca gaggtatcca cacaatattc tgtctgcata ccacataaaa
    15361 ctaatttttc aatcccctgc ttatccaagt attcttttaa gccagtttct ttgaaaatac
    15421 tgttatactt cttctgaaag accttttcag caggttttcg atttaaaagc gcagataact
    15481 gccaatcttc tgatgtttga gcttcagagt tctcaatatg ttgaacatag ataatttcaa
    15541 tattcttgct tctagcctgg ttttgtaaat aagaaatttt tcccaataga ctttttgtct
    15601 ggaacccagt ttctactaaa atattctgaa catcaatgat tataaaagcc gtcttcattt
    15661 agttctcttt caaatagtgc acaaaggcgt tataggtttc aagggtttgg ggatagatgg
    15721 ggaatccctg atgagctaga tactccacgg tacgagctgt ttcgtaggcc accgccttat
    15781 tgagcgatag atttgcaatc tcacgcgcca catctactcc aggaaaggct cgattgtgct
    15841 cgatatagtc tgcgacgtag atgaccttat ctaggtctgt catttgacca gcaccgactg
    15901 tatggacttc aatggctcgc aggatttctg aatcatgcaa atccaaatct tcctgaattt
    15961 tgtaaattcc gaccatacca tgccagacat tattgcccca gtttttgagg tcagggtcta
    16021 gctggtaacg gtcaatcaag gctagaaatt cctgatttga caacttttta gcatagtcat
    16081 gaagaagacc tgctagactt gctttctcaa catctacacc aaagcgctga gctaattcca
    16141 tagcggcacg ctccacacct agacaatggg ttagacgttt ctcaggtaga aactctgcca
    16201 ttttttccaa caaggcctca cgggagcagt tgatatagtc ttgataggac atcagtagag
    16261 cccctccttc tcgatgtagt ctagcactgg ctgaggtagg agaaagttgg gtttccgacc
    16321 ttgggcaagg aagtcccgca ccatgctgga cgagatatcc atgagcggta cgtccaccca
    16381 gataactgga taggaagtcc ctaccttgta gcgtggacgc tgaaccccca caaactgaac
    16441 catgtcaacc agttcatcaa ttcggtacca cttaggcaga tagtcaacca tgtcggcacc
    16501 gatgataaag taataatccg tatctggatt cttctctgtc aaaatcttca tggtgtcgta
    16561 ggtgtaggaa atacccttgc gctccaactc aatggtttca atgactaggc cgtcaattcc
    16621 ctcaattgcc aactcaagca tcttgagacg atggtgttca gggatggttt cctttttatc
    16681 aacgtgagga ggttggtatt caggcatgag aagaacttga tccagtccca actgttgccg
    16741 tacttgatcc gcaacaatga gatgggcatt gtgaacaggg ttaaaattcc cccctaaaat
    16801 cccaacttgt ttgcgttttt tctccttgat ttctggctcc aactctacct tggtaaaggg
    16861 agtcaatagt tcgattgtca taggctagtc ccctttattt ctctataaaa acagttattt
    16921 ggagtttcgg tcttagattt ctttgacttt cttagaaatc ttgcgatttt ctttcttgct
    16981 agattgctta aacaaaatca agatgcgtcc tattttttgg actgtatcca caccgatttc
    17041 ttcttccaaa atttcagcta cttcgtggat gttttcatct gtgttttgta agagagtaac
    17101 cttgattaat tcacgcgcat caagagcttg acggacgctg gttttgattt ggtcgttgag
    17161 tccatttttc ccgatttgga tgataggttt gagggtgtgt gcctggctgt tgaggaaggc
    17221 acgttgtttt gatgctaatg acataatttt cttccttgtt ttttgatagt tattttaggt
    17281 ggtgagggac ggtcggaact aattttccaa agatttcatc tttgaaaaat ggatttcctg
    17341 acctcacaat tgagccttgg tctcaattgt gccccttgcc aatctacaga ttggcaatca
    17401 caccacggca ataactatcg ctatatgagc tcactttgtt cgctcagcga ttttgtttta
    17461 aataattgct tttcgtgtgg cgacggcgac gccttctggt gcccagacgg cgacttttgc
    17521 tgtgcctgtt acacgaatcc agcctaggcc tgaaatgact aggtctgtct tatctttaat
    17581 ggtaaagaca tgctggacta gccttgggaa atcttctttt tccttgctat ttggtggtgt
    17641 cagaagagtt cccaggtgct tatcgtagaa agcactagct ccttcaagct tgctacgatg
    17701 gagtttgagt tcattatcaa agaaagcagt aaatccttgc ttttctcctg ctataaagtc
    17761 aaagcgtccc aaaccaccta aaaatagggt ttgctcagga ttaagctgat aggtcttagg
    17821 cttgatttcc tttttaggac tgacatactt gaggtttttg gccgtcaagt agtgagccat
    17881 ctggtggcgg tggataattc ccggcgtatc gtaaatataa gatccgtcgt caagcggaat
    17941 ctctattttg tccaaggttg tccctgggaa gcgtgaagta gtgatgacat tctgatcacc
    18001 cgtgatttct tggataatag cattgattag agttgatttt ccaacgttgg tcacaccgac
    18061 cacatagaca tcgcggccct tacggtagtg ttcaatctta tcaatgactt ccttaatggc
    18121 atgtttattt tgtgctgaag ttaggaccac atcgactgga cgaagacctt cttcatgggc
    18181 acgtttcatg agccactggc taatcttacc agacttaact gacttaggaa ggatatcttt
    18241 tttatttcct accaagagga catcattgcc cgagacgaaa cgtggtaaac ctgggatgac
    18301 agatccatta aaatcaaaga tatcaatgac attgaccact aaggcatcac tgtctcccac
    18361 ctcgtgcaag agcttgagga aatcatcgtt cgtcaactgg acatctgtga tttcattgta
    18421 gtggcggaga cggaaacagc gttggcaata gacttcgcca gtctccaaac ctttttcaag
    18481 tgccgactgg ggggtaaaac caagaccagc tttatctgtc gtctgaatgg ttgctccaca
    18541 accaatacag agaatttctt ccatagttaa attccttttt tatatgtaat cggtccgtac
    18601 ttttcagtga tttttctcat aacacgacgc tcacgagttc ggttaatctg cgttttgatt
    18661 gagtcatgtt ggaccaaggg tttgactaaa attgaccgaa tccctgcacg gtgggctgct
    18721 cgtatatctg tcatgagctg gtcgccaacc atgaccactt cctttttgtc atagtggaat
    18781 tccttcatgg cacggtcaat accaaatgtg aagggcttca aggcccagta aacgtaatca
    18841 atcccaaatt tctcaactgc tcgttgaacg cgttttttgg tgttatttga cactacgata
    18901 atgccaatac ccgcgtcccg aaggtcatgt agccattgct tcatctctgg cgttccatca
    18961 gggttgttcc aagcaatgag ggtattatcc aaatcgacca aaacagcctt gattccctgc
    19021 gcctgcaggc ttgggactgt cagatcatag actgcttcca cagcaaaatc tggtatataa
    19081 ttttcaatcg ccattctggc tccttttctt aacttttttt cgcaattttc cttagtaaca
    19141 aggataaaac aatccacaaa cctgcactag ccaaactgat atagagccaa gcatggggct
    19201 catctgttaa aggtaaggga acattcattc cgaaaaagcc tgtcacgact gccaaaacag
    19261 ctagcaagac tgaaatgata gtcaaggttg tcaaattgtc attcagatta ttgtttagaa
    19321 tattgttgta agaggctgaa agctgctgta aaatctgaga gattaggtct gtcatggata
    19381 ccagctgatg agcctcaatc atggcatcat caaactgttc tctctcaatc tcatcaaaac
    19441 tacgatacaa ggcatgacct tgaatatgct ctaacaaaat ccgattttgt ttggcagctg
    19501 ccgtcagata aaccatacca gtctccaaat cagacagggc aaagaggttt ttcttggtag
    19561 ttcgctggcg caagaggtca ttgacctcat ccctactctt gtccatctgc tcaatgacag
    19621 gatagtaggc attgctgatg atttccagac tggcaaagag aaacttataa atcgaaagcg
    19681 tgtcatggct ctccagataa cgagtcatct gttcaatgac ataggcgttc ttggtattac
    19741 taatggtaat caggcgacga tgctcgacga taaaggtcat gggaaaagtc tcatagtagg
    19801 ccttgtcctt ttttacgtct aggacattat agataaaggt aaccgtccca ctttcacggt
    19861 ggtagtccat gtgggcgcgc tcgtttctat ccagtgcgta ttcaatggtt tccttgtcca
    19921 aaccgtaaat ttcagaaagg tcttctagtt tattcaactt tcctaggtct aggtctatcc
    19981 aggtacaacc atggcccaac tgcttttcta aaaccatctt ttctccttat taaactccct
    20041 ctattgtacc acaaattact taaaataaca ggtctagtct gtgcgtgaga aattgctaac
    20101 aactgtgata tttcggttag ggacaaagtg aagggcttgc tcctcgctac gaagctgtgt
    20161 cgtacgaatt cccacactga caaccttacc cgatacagta atcggtccat ttgtcagaac
    20221 gacctcatct cccacatcca gttgacgttc aaagaggatg aaaaagccat tgatgacatc
    20281 agacagaaag ccttgggctc ccataccaat cgctacccca gcaataccag ctccagccag
    20341 caaactagaa actggcaaac ctaaaatcga caaaatgcag tagagtaaaa agaaatatag
    20401 cgtataatta aacacatttt ctagtaaacg tgagatggtt ttttggcgtc cgacatcatg
    20461 acgagacatt tttagagaag gtttgacaat tctctgcacc atggtatgaa gcattttttt
    20521 agcaatataa aatacaatca aaagcagtaa aagagaaatg accttggtta gaatattctc
    20581 gataatggtt gtcacatcta gcttttcaat ataagcctga ataaattttt gcatataaaa
    20641 ctcctttatt tcattatacc atattttcat cagtctcatt ctccataaat attcaaaaaa
    20701 tagacagaaa attcttgatt ttgtctatca tttatactat aatcataatc actacacaaa
    20761 aaggagatta ttatgaaaag aatcattcat gcttggaata aggcaagcct tgtcaagcgt
    20821 atcttgattg gtatgctttg tggggaatcc taggactgac ccttcctaat ctctcaggga
    20881 ttgggctact cggggattta ttcgttggag gtctaaaagc tgttgctcct atcctagtct
    20941 ttgctctcgt tgccaatgcc ctttcccaac atcaaaaggg acaagatagc aatatgaaaa
    21001 ctgttgtttt cttgtatatc ctatgatagg gactttcgcc gctgctcttg tagctgtact
    21061 agcaagtttc atcgtcccta ttgaaattac cctaaatagt gccaatactg aaattgcacc
    21121 accagatggg attgggcagg tcctcagcaa cctcttgctc aaactggttg acagcccagt
    21181 caacgccctg cttactgcta actatattgg aatcttatct tgggcagtca tttttggaat
    21241 cgctatgaga gaagccagta aaaatagtaa agaattgcta aaaactatcg ctgacgtgac
    21301 ttctaaaatt gtcgaatgga tcatcaatct gactccattt ggaatccttg gtcttgtttt
    21361 taaaaccatt tctgacaagg gagtcggaag gcttgccaac tacggtattt tattggttct
    21421 attagtaacg actatgcttt ttgttgcccc tgtggtcaac cctttgattg ccttcttctt
    21481 tatgagacgc aatccttacc ctctagtttg gaactgcctc cgtgtcagcg gtgtgacagc
    21541 ctttttcact cgtagttctg cggctaacat tcctgtcaat atgaaactct gccatgacct
    21601 tggactcaac ccagatacct attctgtttc tatcccactc ggttctacta tcaatatggc
    21661 tggagtagcg attaccatta accttttgac ccttgttaca gttaacactc ttggaattcc
    21721 tgttgacttt gccacagcct ttgtcctcag tgtggtagca gctatctcag cctgtggtgc
    21781 ttcaggtatt gccggaggtt ccctccttct tatcccagtt gcttgtagcc ttttcggtat
    21841 ttctaacgat attgccatac aaattgttgg ggttggtttt gtgattggtg tcatccaaga
    21901 ctcatgtgaa acagccctta actcttctac agatgtcctc tttaccgccg ttgccgaata
    21961 cgcagcaacc cgtaaaaaat aactcatcaa ggcaagcctg ctcatgtctt gtcttttacg
    22021 cttttattct aacttattag gaaattctta tgtctattag ccaacgtacg accaagctca
    22081 tcttagctac ctgtcttgcc tgcctgtttg cttattttct caatctttcg tcagcagttt
    22141 cggctggaat tatcgctctc ttgagcctat ctgatacgcg tagaagtact ttaaaactgg
    22201 ctcgcaatcg tcttttttct atgcttctag ctctggctat cggtgttcta gcttttcact
    22261 tgagcggatt tcatatctgg agtctcggcc tctatctggc cttctacgtt cctttagcct
    22321 acaagatggg ctgggaaatt ggcatcacac caagcacggt tttggttagc catctcttgg
    22381 ttcaagagtc aacctctcca gacctcctag tcaacgaatt ccttctcttt gctatcggta
    22441 caggatttgc cttgcttgtt aatctctata tgccttcacg agaagaggaa atccagcact
    22501 accacacgct ggtggaagaa aagttaaaag atatcctcca gcgcttcaaa tactatttat
    22561 ccagaggaga cggacgcaac cgagcacagc tggtagcaga attagacacg cttttgaaag
    22621 aagccctcag actggtctat ttggatcact ctgaccacct ctttcaccag accgactacc
    22681 atatccacta ctttgagatg agacaacgac aaagtcgtat cctgagaaac atggcccaac
    22741 agattaacac ttgtcacctt gccgccagtg aaagcctgat cttagcgcaa ctcttttcaa
    22801 aaattgcagg tcaactgagc cagaccaatc ctgcttctga tttgctagat gaaattgaac
    22861 gttatctgga agtcttccgg aaccgcagtc tgcccaagac aagagaagaa tttgaaaccc
    22921 gcgccaccct tcttcaactc ctacgtgaag ccaaaacctt catccaagta aaagttgatt
    22981 tttaccaaaa atatagacag taatactctt caaaccacgt cagcgtcgcc ttgctgtata
    23041 tatgttactg acttcgtcag ttctatccac aacctcaaaa cgatgttttg agctgacttc
    23101 gtcagttcta tccacaacct caaaacggtg ttttgagctg acttcgtcag ttctatccac
    23161 aacctcaaaa cggtgttttg agctgacttc gtcagttcta tctacaactt caaaacggtg
    23221 ttttgagctg acttcgtcag ttccatccac aaccttaaaa cagtgttttg agcagcctgc
    23281 ggctagcttc ctagtttgct ctttgatttt cattgagtat aaaaaagaaa acttcagacc
    23341 aatccacaaa aggtgaatat ctgaagtttt cttttattta taagtaatca ccataaaggt
    23401 taatagtaga acaaataaag ccaaactcac caataaagtg agcaccttga ccaaggtgtt
    23461 gctctgccag tagcttggtt taccaatatt actagtggca tccatggaaa atagttgatt
    23521 ttgacggggt tgtatagtta ccaatacaat caaagcaaga gataggataa tagctaaaac
    23581 aattagcatt tcaatcattg atctacctca aaattcccaa caaagtaaag agcaaaccaa
    23641 tcagaatcat taagcccagt aaaagtgaaa aagttttact aatcttctcc cctcgggttt
    23701 ctttttcctt cttgatgggt ttttgtttca actcttccat gcgaccttca acgtctttgt
    23761 aaaataaatc ttcctctgac atgttagcct cctaaaacag tttgcattat ttcttgatat
    23821 cgcaccaagt caacataatt tgcatgttgg ccttgtttgc caagtgcctg acgcatttga
    23881 tcaacatctg aaagggccaa tttaatggtc tcaaccaagg cagcaacttc actactttca
    23941 aatagatgtt ctggagcgat ataaagtcta ttgtgaaccg tttggttaaa tccaagaatc
    24001 aagagattgt gctcaaaggc ctgacgcact gcctgcaaca actcattgct gtgattgata
    24061 tccaagtaaa tatccgacag ttgatacagc tcctgaatct tctgtggact agcattctgg
    24121 taaaggacca cattaggata gcgaagcatg tctaagagct tagaagacat ctctgtcact
    24181 gctgcgatac ggaaggtaac atcaggcaag gcttctacga ttgcttctac ttgctcaatc
    24241 tgatctgaat tggtcaagat taaggcatct cgtcttagga aattatcacg tttgaactgg
    24301 taatgataac ccaagtgcac aaactgatca tggtatttct cgtcagttaa ctctaaagcg
    24361 cgctcataag tcgccttatt tggaatgatg atcttcttag tacgcacatt atcactttcc
    24421 aaaatcaact gcatattacc tggaatggca tcatagagag gttcctgcca taccaagaca
    24481 tccgagccag atttatctgg atgatggaag gaaaccaaga aaggagtcgc tagagtatta
    24541 aagataagct gactggtatc tatttccaaa tcttgcaaaa agaaggtgat aaattcaact
    24601 ttatttgcaa agtaacgcat ggactgacca ggcaaagtca ataagatatc acccgtcaca
    24661 tggttttcca gtaaaacttg ttggccattg acatcttggt aaactgtcat aatcggctcg
    24721 ctatctgcgc tataagtggt tttagcaaag caagcaccaa agcgattgta gtggtcaacc
    24781 tgacgtactc gaccttctag gtctttccag tctacctgtt taaccaagcg agcctgcatt
    24841 ccatcaacat aatgaatgac agccctctcc tgcgtcacat cttcaataca agcagactga
    24901 ttatctccta aaatttccca aaaatctgaa acgggaactt ggttaaaata gagaggtttt
    24961 ccatcctcgt aacctagata ataggtaaaa ggagaaagca gaccatcagg cagaaaacca
    25021 tctgcatcga tgaccactcc aagttgagaa agaccagtag cgactagact ttcatgtaaa
    25081 tctctacttt cttgactgta actatcataa agttcaatca tggagcacct cctctactgt
    25141 tttcttccac ttttctaaaa tttctttggt caagaagcct tctgcaattt gataagaata
    25201 ggcacgcata gcttccaaac gattttcttg atacaattga caaatcttag cggcataagc
    25261 ttgcttgatt tggtcttcta catggtcaga tgaacttgga atcaaataac cattttgccc
    25321 atcctctata aaggtctgat taccataagg cacatcaaaa ccaattagag gtagacctga
    25381 accaatagct tccatcaagg tcagaccaaa tccttcgctg gtagaagccg ttaagtagac
    25441 ctcatactgg ctataaatct gcgaaagttc cgcatgcccc ttgagttgga tatagtcctc
    25501 tgcctgatga tttgcaataa tttctctaag cagagaatct tctccaccac taccatagat
    25561 atcaaaggtt agttccggta actccttatg agcttcaatc acagctttca caagccaatc
    25621 aatgtgcttt tctttggcaa gacgtgaagc cgtaatcaat gaaaatggtt tgcgcccttg
    25681 acttgaatct gtcaaggaat caatactgcc tacaggaatg gtaacaatct ttggctgatg
    25741 ctgagtatat ttggcaaatt gctcttgtag aacttcattt tgtctatcag tagacacgat
    25801 aaagaagtca accttatctg cattggtaaa ctgatagtca taatagttat tccaaaggat
    25861 atagtcctca tttgtagcat tttcactata atgctccgca tgaacaacta ccgctagatg
    25921 tgctgtctgt gcttcctcaa acacaacctg tccaatacct gtctccctat ccagaataac
    25981 caaatcagac ttgttcaaat tcaaagattt cataaaggca cgcacaaaag cttgctttcc
    26041 atagaaaatc ttatccttga aatgataaac ttcttccttc ccttgattca tcaaaatatc
    26101 atagactgga gtcccgtctt cattgtaaaa agttcgttgg tataagactg caacattgtc
    26161 cttgggagca aaatactcgc tacaataacg cgtataagaa aagtaatcct tccgaatcag
    26221 gtttccctta aaaacatact cggcatgttg aaccaagtcc ttgttctcat caaccaaata
    26281 acaggttaca aacttatctt ggtcaaaaaa gaatacacgt aaaaccttgc catttttttc
    26341 tctgtgactt tcttcaccac caaagtaagc caagacatca tccactgtca cgctagtagg
    26401 tgcaattttg atatctgtga aatgattata aagccagata acctgattat catcaaaacc
    26461 aatattggct gttaagtgct gaatattatc ggctaaaatc atatctgtaa agataaactt
    26521 agaggacaga tttaatttcc gaaaaacacc agcacgatag gcttgagcgt attcaacacc
    26581 gctactagcc caaccaattc ctaaatttat attgtaaatt gtcatatttt ctcctcttat
    26641 ggaaaatgag tcacgatttc accttttgga gtgacctcaa ccagccccat caggagatta
    26701 cgcaccatat ccgcacggat ttgttctttc atagcttcaa agcccgcgta ggcttcttga
    26761 tagtactcta cgattggatt tttctgacta gcagattgac caccgatagc catggatagc
    26821 tgttgtagat agtctacctg ctctacccag ttgtcatcaa tggctttaag cagtgaaagt
    26881 cgtaaaaact gttcatataa gtcatgttga ttaagtaatt ctttcttttc agaaagttct
    26941 ttatcaatca cctgcttcat aaagctacga actgcagttt tgtcagttac atctatataa
    27001 tctggaacct ctttaacatg aaaactaata ttggtcacaa taaagtgaaa caataattca
    27061 cgactagcat agtgatcagc cgctacctct tctgtatatc tctcaatgat atccacaaca
    27121 acatcctcta agtcacgaga accatctatt agacgatttc tctctttata gactatatcc
    27181 cgttgtatat tcatactttc agcatactcc agagtctgac gacgtgctga acgtccagca
    27241 ctatcactgg catgctgagc cttttcgact agtttccggt atttacgacc tttcaatact
    27301 tccggttgag tcatatcttg aacctgatag tctttgtact ttttatgcac ccaagatgga
    27361 ccaaatttct tgataacatc atcctctaag gatacaaaaa atttactcat accaggatct
    27421 ccctgacgac cagaacggcc acgaatttgt aggtcgatcc gctgactttc catccgctca
    27481 gtcccaataa caatcaagcc cccaagctct gcgactcctt taccaagctt gatatccgta
    27541 ccacgtcctg ccatagaggt agccactgtc acagccccca tctgacctga ctcggagata
    27601 atctgagcct cacgcgccgc attattagca tttaggacat tatgggcaat cccttcacga
    27661 aacaagagag acgaatagag ttgagacatt tcaactgagc ctacaaaaac gagtaaagga
    27721 tttcccttag catggtattg cttgatgtac tccaaggatg catacacttt ttcaggtaaa
    27781 gtgatatata gattatctgg atagtcaatc cgttgtctcg gacgattggt tggaatgcgt
    27841 actacagaca tattgtaagt ttcaataaac tctttttccg cgaccttacc tgtccctgtc
    27901 ataccagata tcttattaaa catcttaaaa agactctgat aggtgatcga ggccatagcc
    27961 cgcgtctcag gagataattt gacatgttcc ttggcttcaa tagcctgatg gagacctcct
    28021 tgaagtttag tcatttccat tagacgccct gttcccttat caaccagtac catctcattt
    28081 ccacgaatga tatagtcctt atctttagta aagagcttat gagctcgaat cgcataaacc
    28141 aaatgacgag caaaagacgc atgctcttcc ttgtataaat tatcaatccc taggaaattc
    28201 tcagcagact tggccccctt agtagtgagc caaacctcct ctttctcctc tttaaagata
    28261 taatcctctc cttcgaccaa ggttgttaca agtgtatcaa tgatcgcata gtaattagac
    28321 tgaacacgag gagaacccgc aataatcaga ggagtttgtg cactatcaag caagatatca
    28381 tcaatttcat caataataac atagttaaac ggtcgtaaaa acttaccttc ttcattcgag
    28441 gctaggttat catttagata atcaaaacct aaactactat tggttgtgta gatgatatcc
    28501 gaagcataga taagcttctt ttcttcagct ttcatttcct tctttggatc ttccgtaaat
    28561 ggtacaccaa tggtcaatcc tagaaaacga taaacttggc ccatttcctc ggcatcacgc
    28621 tttgataaat actcattagg agtcacaacc atcactcctt ctcctgaaaa agcgttcaaa
    28681 tagacaggca tggtagctgt caaggtctta ccttcccccg tattcatctc agcaacattt
    28741 ccatagtgca tgacaatagc tcccatgact tgaacatcat aggggaacat ccctaaaatc
    28801 cgcttatctg cttcacgcac cacagcaaaa gcttcaacca aaatatcgtc tagactttct
    28861 ccctcggaaa gacgctgacg aaactctact gttttagcta ctaattcttg atccgaaaga
    28921 gaggacatct tgcctttcag ggcattaatc tgctttaaaa tctttttcac tttcctaagc
    28981 tggaaatctt gacctaaacg tctaaacact tctgatctcc ttgatagaaa aatgataaaa
    29041 agttaaggac tcaaatccag cacttaaaag agaaacttga taagtataac tatcttctgg
    29101 ataagtaaat atgaaatctt tcgctttttc tacctttttt tctaaaatat caccatagcg
    29161 gtcaaaaaaa gttatcttgg tataaactcc attttcaggc tgacaatcaa aaaccagtga
    29221 tagttgatac tcctgctttc tttttaataa gggaagctga ggttgggttc gtgcagcctg
    29281 gtaattgaca ctagaaaacc aagtctttag aatctcacca gatggcaata gaggattata
    29341 cagacgaaca cttttgtcaa ggtaataaga tacagttgtt ccatacatat aagtcccacc
    29401 aacttctccc caataaatag actgtctctg tgtaataatc atcttttctc ccttccaaaa
    29461 ccagtctcta agaccattct ataaaagtgc aaaaaccaag aaatattggt atctgtatca
    29521 tcattgtgtc gtccagcagt tcctttagat aaaatcttcg cacctgtatt acacagataa
    29581 gacactaatt gttcataggc ttgtggatcc atttcttcat ctttcatata ggataaacca
    29641 aaggttgtct ttgaaaaatt tgcttgttta aaaattttcc aaaaacgctg atccaactcc
    29701 gtcatatctt gagaacttgt cccacctgta tgatgaatta aacaatcaaa agctaagtta
    29761 gagacacccg gtgcgtccaa acgtccacga ctagctatag ttcctaaatt agccaagggc
    29821 ttaccaacta tgatggcatg gggttcaaaa gaagcaccat aatagagagc cggaaacgtt
    29881 cccatagaaa gacctgataa aatcaaatcc ttatgatcaa agcctaaata atcaagatag
    29941 tgagtgatcg tatcctttac ttttccctct agctcatccg ttccgagata aaaagctccc
    30001 ccctctaaac gtggatcaga aaataaaatg aagggacatc caagagtttt catcataaag
    30061 taaccctcga atccttctgc aggtcgataa cctgcaaaat agacagtcaa aggcggttta
    30121 aaatcacctg gatggaagaa atagtttatt tcatcacgct tggaatcatg taggatattt
    30181 ccaccaagta caaacttacc aaattgtttt cgactccatc gttggtgaag attacctagt
    30241 ttgatagttc cacgacctct tgcttcaaca gatatcgaga gaaagtagtc tccgtctcga
    30301 ctatccataa aaagtgcttg ctccaagtct ttttcagata ctattacttc ttcaaagaaa
    30361 tcatcaacag acccactcca cattttacgg attactaaac gaaagtcaca attgccttcc
    30421 ttctcatatt caagccacaa ctcaataggg agtgtttttt gcaccataat attataagcc
    30481 caataggcaa gctgggcaaa atcttgccca aactcacctt ccaaactgac ataatccaat
    30541 ccttgataag aaatagctcc tgtaaaattt ggattgactt gtatcgtcgg aggaaagagt
    30601 ttatcaccat acccaccgcc aaaaagagat ttacttaaat cattgattag ttgttgggga
    30661 cttaaaaaat caattgcttg tgcacatcgt ttttttagaa aatccacaac ctctctatca
    30721 tttatttgta gattctggtt atagaacaaa gtataaggtt caaaatattt tacataggaa
    30781 aataagtctc tggaatactg accatcttct attaaaactg catgaaaacg gttaatatcc
    30841 tctttttcga ttatttcacg aagggttttt gatgagtttg ggtaaaaata ataccaactc
    30901 atattttcag gtatgtcata gtgattttcc caattggcaa gtcctatctg taaaatatta
    30961 agttcctttg acatttttaa cctcctctat ccatttctct aattttccaa gaaattgttg
    31021 tcctgtgtgc tccttaatct tatcaattga atatatcaag gactcattcc actccttcaa
    31081 cctatctgtg taataatgtg cagccttaga aaattctgtc acatctgtca gcaaataacc
    31141 attttttaaa tgttccacat agacggtttc aactaggttg atttgaggaa ttcctgcact
    31201 aatcccagca atctgtgtgt agagatgagg atgtctattt aaatccacaa tcaaacggac
    31261 aaattctagt gtttttatca aatctaactc atcattcaag ttaacaaact gtaagcgtaa
    31321 gtcctggtgt tgattttctt ccaggggatt ttctgcatca ccataatcaa tcgcctttcc
    31381 cagattttcg gattgaatgt tttcttggat gaaagactca acaatccctt caacctcatt
    31441 catttgctcc tgactagcag caaaggctcc aaaaatcacc tcagtatcct tattttcagc
    31501 gacaaaggac aagacttgaa gcagagcttg attatcaatc ccctgctcaa aatccagttg
    31561 aaaataaatg atggattctt tcttagtttg gcttcgtccc aatcgtaatc gggtgtcaaa
    31621 agatgataaa tgataacatt gatgcattcg ttcaggatac aactcctgca acaatcgtaa
    31681 actatcctct ctatccacca aaatcaaatc cgatttttca aaagttacat ctaaatccct
    31741 aaaggtatct tgaggattac gtccaatgaa cagactcaac agtttaggat tagtactagg
    31801 taaacgatct agtaccaact ggtcatgatg agaatgagaa ggaagcataa aaatatcctg
    31861 atccttcaca tacgtttgca gataattctc aaaaaattct gccaccagat ctcccatatt
    31921 ttgataggta agcattttaa agcgataacc caaaattgga ttgacttcta cctgtcctcc
    31981 ttcttttaaa cgctctctaa attgccaaac tcccttagga ttcaaatatt cttgataggc
    32041 tgcttgccca tcttcaaaaa aaataatact cgatataaac cctcgatcat ccatgatatg
    32101 gtgcatatgt atttgaccat ttggttcaaa atactgtata tcactgatga agccttcaac
    32161 tccatgttca accttagcaa atttcttccc atttttttga acgataatcg taaagggact
    32221 ataaataaat tcacagtcat catcccactc aatatctcta atgcttaaaa cttgggtatg
    32281 gagattatga aaatcttgca taacatcaaa aacggaataa gtatccgttt ctaacacacc
    32341 atgtctatat aaaaaataac gtagatgagg ctggtaagct aatactaata gacgagaatc
    32401 tatatcttgc tcttgaaaga gccggatctg gtgaaaggta tcatcaaact ctagacgaaa
    32461 atgagaaaaa taccatggag tgatatctgc atgccatgtt ctttctgacc cataccaagc
    32521 tggaataaaa taatacataa aagtctccta aatcaaaggt ttataggtct catttagcct
    32581 taaagccttg acctcgtctc taatcgtaaa aatcatgcct ccaaacatca taaacaaacc
    32641 tggaatcatt gccaatcgta ataacttttc atcaaacaaa acaaaaagca tgggaccacc
    32701 tgccataacc acattaaaga gaccacctat gactgagaaa cgaaggacca atcgattaat
    32761 aaatcgacta gtatccgcac ctggataaat accataaatg tattctccag attttttcat
    32821 acggtctgca atctcttctc cattcatcgt aacaaaagca aaaatgatac taaataaaaa
    32881 taagaccgaa atataaacat agacccacaa aggctttcca atcataaatt cctttgataa
    32941 agccgctaac cctgaatgat tagggaaaat aaatcccaac aagatgaaca aataagctgg
    33001 tacactaaga aaactcatca catacatata aggcatccca cctgcaggat tcaacatgat
    33061 ttcgagataa aaatagcgtt taaatcgatt gtgtaagcca attttattaa caggaaccaa
    33121 atagcgagct cgatacataa gggcaagtaa ataagaaaag acaagggtta ataaagcaag
    33181 taacacaata atccctgttg gaatgtgtac tgtctgaaat gtttccaaaa catcctgagg
    33241 aatatttaaa accatactgg ataggagaat gactatagaa cctccaatcc ccatactcgc
    33301 atttaaatct gacaaccaaa caagaaaaaa tgttcctgct atcagcaata ttgtattcat
    33361 tagaacaacc aatattgcag aataggagga ttgtactggc agtctcaagc taactgccaa
    33421 tgattgaatg acagctatca tcaaggtcag gtacatttta cggcgatctt gtatttctat
    33481 agacgtagat gttaaaccca accgtttaga aaaagaaaac atctgccata aaatcatagc
    33541 tgacatccaa ggggacaatc caacagaaaa aattgacaaa cttcttagat ttccacctgt
    33601 tagggcggct gagaaggcta gataggctgt tgaacctcct aaaaaatctt tagtatttag
    33661 gtcaacaaaa gggagagtaa tacgacttcc caatacatag ataaacagta gaaataaggt
    33721 aaatagtcct ttttttactg ctattgacga ataaatctta gtcataaaac actcccctca
    33781 ccttcattgt ttgtgatatg gtaaataaca attcttttat ttcaactttt accatttctt
    33841 tgttctgcat caaaaaacaa ctttaatctg aaacaaatat ctttatattc ctcagtatct
    33901 gttaattgat ggtattctaa agtttgctta taatctttta aaagattgac aaatcgaata
    33961 cgcagaacgg agacatccaa accagccaag acacaatccg atattttctt agaaaacact
    34021 tcaacaatat ctcttgccca ggaaagatca aattccgttc tcatgatgct tcctgaacgt
    34081 cttctataca gatagagatt atcgtttata aagacgattt tagaggctaa aagataaaag
    34141 cgatgcatag ttgcttcatc atcaaagcgg cgaccatttg aaaagtgaac ttcattgaat
    34201 aattcttttt taatcaactt aaatgtcggc aatataaagg ccgagctatt gaatttccaa
    34261 tctcctgctt gacggttcat aatttcttgt atagctaatt cttcgacgca aaaatcttgc
    34321 cctgttacat aaaaataaaa attcccgtca ctttcgtcat aattatagta attccctata
    34381 acaacatctg catccttttc ttggacttgc tgataaagag tttcaatcat attttctttt
    34441 acgatatcat ctgaatcaac aatcgtgatg tactctccct tagctacact aatccccata
    34501 tttttagctt gtgctgcccc cccttatttt cttgatgcaa tactcgcaca cgattatctt
    34561 gcatcataaa agcatcacaa atttccccag aattatccgt agaaccgtca tcaactaaaa
    34621 taatttctat attttgatag gtttgcttca gaatatgctc gacacactcc actaaatatt
    34681 ttcccgtatt gtatatcggt accacaacac taattaattc actcattact ctatctctat
    34741 attcttcatt ctatccacta aatcatctgg tctctccaca gaaaaaatac cgtcatagag
    34801 tccatcatcc atacttttac gtgtgatatc aaaagcgaag attttcttgc cactttcttg
    34861 tgcccttgta actacattaa aaacctctcc acctgtatta atatctaaat aaactataga
    34921 atcgtccaat agccaatcaa ttctactatg taatacattt tgatatactg ttacattcgt
    34981 atactgtgat agagaggtca gctcctcaga acaatcacac ggtgcagcca aatgaaaatg
    35041 ccaatcaggt aaccgttgta ctaaatactc taaatgtttt atatctgcac tccatgtcac
    35101 aagcatcact tgttttttag actgattgct tctttcaaaa taatttaaat cggaacgttg
    35161 aaaagcaatc tctgaccaat ccaaatctct ataaacccac cataattcac gtagtctaga
    35221 tatactatat gtattccaag gtttatcatg actagcatag tgaacaattg taggtggatt
    35281 gtcatctaga cgttcacatt cttgagcaag gtgataaata tcaacaccca ccatataatt
    35341 atatgtttta tctaaggcta gccaattatc ctcaaaataa atatttaaaa tactctgatc
    35401 cccaagatga acaacttgat tttgctcggc cgctaattcc aataaactat tgacaataga
    35461 gtgttctttc cattttgcaa catccattag taacatacca gcattaaatc cagattttcg
    35521 tccttcatag gcatagacat catcaacagc accaattgaa tatcctttga gatctatctc
    35581 aaacaaagta gctagttccc cagttacaat gatatcggaa tccagataca ataccctatc
    35641 agattcaacc acttctgtcg caaaaaatct aaagtaagaa gcataattta tatgaggtcc
    35701 tgttttatag ctttcaaaaa gttctttatc aatgtgtaca ctgcgaattg tagaattcac
    35761 aacttccatt tttctcccca ataatttaaa ccattcagga gctatatcac tgttgagaat
    35821 ataaaaatca acatctctat tgtgatacaa tacagacttt atagtcgtct ctaaaggaat
    35881 aagataggca ttatctgccg ctaatactat tgattttctc atactgttct ctacctcatg
    35941 taagatctga ttgctgcaac catttttttc ggaatggagc ttggatagat accttcatat
    36001 gaaatctcag aaagattttc agagcaagta ttgtcgaaag ctatcattgg ttttttgtac
    36061 ttaagcacaa actcatatgc atcttctaat tttcgatcat gattcaaatc caaatacaaa
    36121 tctgaagcta ttactatttg ttccaataac attggtaagg aatttggata tacggtaaca
    36181 ttaggaaaac gcgttaaagc tagtagttca tttgccatat ttgtataagc aacaatatga
    36241 aaatgaactt ctggcaattg ctccgccaaa taatcgattt tctcgacaca ccaagaattc
    36301 gttaaattaa cacattgtaa cttaaatatt tgtgatttac taggatactt cacactcttt
    36361 gaaaaccatt catttaaaat aacagaccaa tccatcaaag agtattccca ccaaacttct
    36421 cttagacgtc caacagaaaa ttgattccaa ggcttatcct gagaaatata gtgtaaaatt
    36481 agtggcagtg gttcgagcgg aatatcaaaa atgaattgat gtttaaaggc tgccgcccca
    36541 taatcatatc ctatttgaaa attatattga tcttcaaggg aactatattg atctttaaac
    36601 aacatattca aaattgactg gtctccttct tccacattct catgttcttt ttctgttaag
    36661 tcaatcaatt tttgtcgaat agtttcagat ccccattttt tgttgttaat caagagaaca
    36721 ccagcattga agccgactcc tgctccaaag caagaacgag ctgctgccaa ataattttca
    36781 cctaagtcta attcaaacaa atcggtcaaa tcaccagtca caatcaaatc actatctaga
    36841 tagagaactt tatcttctgt tacaaaatct ggaataaaat agcgtgcaaa tgtcatatga
    36901 ttgatatgag gtaatttatt agaccaattc atttgaaact gtgagccaat taacttgcag
    36961 tcaatcaagt cgccccccat ctcttgtaaa tatattctta tttgactaaa ccattcctga
    37021 ggaatgtcct gatttagcag ataaattttc aaatgactat tgtgtctaca gagtgacttc
    37081 atcgccgttt cgatttgtcg aatataagcg taatcacctg caaatgctac agcgcgtttg
    37141 gtatttctca ttttaaatcc ttatcattta ttttaaatag tctagtttaa tattcgtgca
    37201 acaatctgac tgttataggt ttcatcatca ataaattcaa tatcttgatc aaaattatac
    37261 tctgcagtat gtttatgaga aaatttaacc ttagttttag ctcccatttt catccattca
    37321 tactctgagt caatatggcc tacatccaaa acttgatagc ccatctggca tagattataa
    37381 ctcagaactt ttgctgtagg acccagcata cataaaatca agcgaccacc agcatacttt
    37441 tcaatttctt gttcaatttc atgaactcta gaaaaggcac tatgagaagg acagataatt
    37501 cgcttaatag aatttgcctc atcgaataaa tcatttccga cacctgaacg agaagtcgca
    37561 ccttcgacta tcagtaagtc acggttttcc caaatgcttt tcaatttttc aaattgagct
    37621 ttagcttgac tcttgtcttc aaaatcgata taagggcgag atacaaaggt tgagccatac
    37681 catgaatcac tgcataactc tctgtaaaaa tccatataat gatccatgtg atctttccaa
    37741 aatggaatcg cccacgatgt aaacctaaaa cgatctgtaa aagcatcagg aaggcacact
    37801 actaaatctt ctcgactttc ttggccgata atgtccctca tgattgaaac caactcttca
    37861 tcataatcct ggtagggaat tgaatgccct gcaagcatat tgatttcccc atctccaaaa
    37921 cgaactaaag aagagttgtg ttcaataata taatccaagg tttcatctat ccctttcacc
    37981 tggattatat caagcggttt ttctagcata tccttagtta tcaatttttg caaatcagcc
    38041 aacattcgtt caggatgttc acttgaatac aacctttggc cattattccc attttgagat
    38101 ttataaaagg ctagtatagg tttagataga tgagccattt ctgcaacgat atcggatgtt
    38161 gaagtcagta ggttgatatc taaataagca tccatcttct ccttcaactc gtctagctta
    38221 tctctagaaa ttgccggata gagctgaata ttaggataac gattagacaa atcatataat
    38281 tttggcccca tagctgtcca agcagcaact tgaaaaacaa catttggcaa ctgtccagcc
    38341 aagtaatcca attgttcgat atcctgagaa gccgtaaacg ttaaacaagt taaacttgga
    38401 agttgtcgaa aacaggtaag atgtccgttt ttcttaaaat ctttaatcgt atcaatcata
    38461 aaggaaactt tcccattttc gaaaacgatt tgtcctaact ctccgtgctg agtcgattga
    38521 aaagctagta gcgttttttc ttgttcttgc aaagatttta aaaagttctc atctgcacta
    38581 ccatgattga tatccaaata tagatttgtt gactttttca gcttatctaa tacaggagga
    38641 acaatttgtg gatgcaatct cacattatca tacacagcta attttatcag cttatcaccc
    38701 atatccgtcc aagcagcaat atgaaatacc acatctggta aagctgtgac taactcttct
    38761 attccttcta aatcttggga atttgtcaaa gttaaacaag aaaattcctt atctaaagac
    38821 gatgtcagtt caaactcccc tatatgatgt tgtaaaattt tagtccattc caaatcatga
    38881 aattcccacc ataaatcacg ataacgattg gctatcaagg tggtccaggg ttttctgtaa
    38941 gacacaaaat gaataatact aggaggtact aactcattaa aataattttc ccatttatta
    39001 taaaaagcag ttacatcatg acccacttga aggttaaatt ctttatccag ctctaaccaa
    39061 tcatcttgta agacctgatt aaaaatcgtt tgatcaccat taaaatgctc gaaacggcct
    39121 tcttcaactt ccttcattgt aacaatgctc tgttcaatta gtcgttcttt taatttctct
    39181 tgacgccatt ttttattgtt gattaataaa acacctgcat taaatgtaat tccatctgta
    39241 tctttaactg ctgcgagtga tttttcttct aaacaaatac taaataattt ctctaaagaa
    39301 gtatttacaa tcaaatcact gtctaaatat aaaaccttat cttcttggat ataatctgca
    39361 ataaaatatc tagtataggt aatgctacta atgtgatctt gcttttccca atcttgaaac
    39421 acagtttgtt caggtagctt cacatcgata atctcactac ctaacatgcg agctattttt
    39481 cgtggtttgc gaaaccaatc tggcataata tcttgattta aaatataaat cttaacatct
    39541 cgattgtggt ataaaataga ttttatggtt gtttctaact gcctgatgta attacgatca
    39601 ccagctaata caattgtttt aatcatcttg ttctcttatt caactcccaa atggtaaatt
    39661 gcatctacca ataatttttt agtaaaataa ccttctttta gcaaataact aaaggttttt
    39721 atccgattcg tcatttcttg atattcttgt agattcattt tatcaactat cgcatgaact
    39781 tcttctagac tattcgccac aaagcctaag ccttgatcta ctataaattt agcagttgac
    39841 aagctacttg gtacaatgac tgggatgccc gctgtcagat aggtactcac cttatgagat
    39901 atatttaagg tataatattg gttgctttct ccatcgtttt gataagttcc ccagacaagg
    39961 ccaaatccac cctttgataa ttctagcaac aattcctcat cttttttcca tccttcgatg
    40021 ctgagatttc tagcactaga actagcttcc cctttatttg aaaatactct caaaggcgta
    40081 tcttgagacc aattttgtaa gtctggaaaa cgctctaaac ttccagcaaa aaaaagttct
    40141 tttttaaaag caggggtgta taaggataaa tcatgaggat gatcccacat cccttgaaca
    40201 agaatcttct tagtcgtcaa tccttcttcc atcaggcgtg ttttcattct ctctgacggc
    40261 actatcaaaa catctgatag attatacatg tacatatagt ctttcatgag ataatagtta
    40321 ctatcaaaca tgaggggaac aacatcatgg ataaagcaaa taattttcac ctgcatatcc
    40381 ttcaacttat caaacaagag acgatcaaat tcaaagccat tccaggttgg agactgaaag
    40441 actaaaatat ccccaataga aatactggcc ataataccat ccagacgctt attcatttca
    40501 gaaggactat ctgaagcaat gttgtaaaaa taaataccaa cctctctaaa tcccagttga
    40561 ctagctatct tttgaacagc attttgagct aagataaccg tactatcacc agccatgccg
    40621 tataaatttg ttaaatgtag tttcatatta cgctatctca ttttttatta ctatctacgg
    40681 aagtaaagac aatttttcca tcattcttct ataaatctct gtgaactgca tattttgttc
    40741 ttctaatttt gctatcactc tttgtaatct atttttataa atcaaaatat gattggtcaa
    40801 gtcatagtct gaacttgcta aaatagctat cttttcttcc ctagccccga tttcatcata
    40861 catacgcttt tcactccatg tattcgataa agtatcagat aaaccaacac ggtaccagta
    40921 aacacaacga ttcaaataga ctatcctcga agctcttaga agtaccttcc atgtccagta
    40981 agtatcctct gcaatttttc ctataggaaa tggtaaatct tgtagtaact ctctcttgaa
    41041 taacttcaag acagctacag tccagttccc atttctgact tcttcgacac cttccctatc
    41101 catgattgct ttaccttcta tcacttccag agaatcatct ggatccgtaa cataagtcat
    41161 atacacatag cgtgtttcat cataagaatt ataacgcccg atactaatat ctgcgttttc
    41221 ctttttcaaa gcaccatata atcggtctaa agcatcatgt tccaaccaat catcagagtc
    41281 tacaaaagta atgtacgccc cccccgaaca ttcaatacct aggttacgag ctgatgaaag
    41341 accgccgttt gctttctcaa aatatttgaa acgagaatct ttctctacaa attcttcaca
    41401 tattttggat gaatgatctg gagagccatc attaaccatg atacattcaa aattcggata
    41461 agtttgtttt tgaatactat ccaaacactg tcgcaaataa ttctcaacgt tgtagattgg
    41521 gactacaata ctaattagtt cattttccat atactaactt tcctagactt gatttattct
    41581 atttccctct taatctcttc tctttacaac aagatttcca ttattataaa ctacactgtt
    41641 tgatggaata tcattgcgta tgagacaacc tgctccaata atggtattat ccccaatcgt
    41701 cactcctttt aaaatcgtga cattacttcc tatccagcag tctctgccaa ctctaatcgg
    41761 cgctgtagtc cactgccact tttcaatctt ttcagcagta taaatatggt cgtgatcata
    41821 aaaacgaaca ccctctccca tcatggtacc agctccgatt tcaattcttt ccatacagtt
    41881 aaaactacaa gagttgttga taaaaacacc atcatgcagt attaacttac cagaagagac
    41941 atgaaaattt tcaaaatttc tgcagatgac atttgctcct acaatgaagt gagccttatc
    42001 atcgatttta aagacattcc aagtcccatc ttggatgatt ttttcttgat gattggtgcc
    42061 tactctgact tcattcagat agtctctcac tctagaaacc aatttgtctg gtgtacttga
    42121 tagaaaaact tcctgcccct gatttccatg gactgtattg tcaaaagcaa agacagattt
    42181 tccagcctgt acaaacttgg agacaatagc atctacttct tgaaagtgat tgatatctag
    42241 taaaaagtca caggtatcga ctagaaagtt tagctgttct tcatttgtaa tcgttcggag
    42301 acggacatta tagttcgttt ctaagctacg gattttctcg gaaaattgaa caggtgctgc
    42361 aatataaaaa cagatatttg gcaaggattc aaggagataa tccaactctt caatctgatc
    42421 actaacagtc aaaatcagag catctcctac taaatgattt ttcttcattt gcttatttct
    42481 ctcaatttct caatcattgc ttgaacttgg tcaacagcat atacctcctg acctgcttca
    42541 taggttttgg tattttcaaa ggacaaaata gtttttccaa gtcttgcaaa ttgattgata
    42601 atttcctctg ttttttcacc atgattaata tctaaaagta cttgactggt ttctaccaat
    42661 tcattatcga catctaccaa atagtgaatt ccgttaaata tagtcacgtt tggataaatt
    42721 gtcatctgag ccaatcgatc actaactatt actctagctg ctatcttaaa ctgaatatca
    42781 ggcaaggatt gaaccaatgt ctcaatttgt tcaatatggt ctgaggcagt atagattaga
    42841 caagtgaaag gttcctttat tggatagatg tgagatcttt gtaatggatg taaatgatgg
    42901 ttttgtccca attctgtcca ttcaagccca tgatagtacc accaaacttc acgataggtt
    42961 tgggccgcca aatctttcca cggtttccga tgagaaagat agtgaataat agcaggataa
    43021 tcctgaccct caggcaattg ataatcagca aactgtttat gaatgacaat atgattataa
    43081 tcaaagtcca attccaacca tttatgttca aaaagcatat tcaagatgct ctgatctgcc
    43141 tgatccacct tatcatgcca ttcattggtt acatcaatta atttttgggt catattctct
    43201 tttttccaaa aagcattgtt taccaagaga acaccggcat taaagatttc ttgaccaaaa
    43261 taagctctgc ccccaaaatc tctaacagca gccaaaggat aatcttgtaa gtctgtagca
    43321 aacaaatcat caagattttt cgttacaagt aaatcacagt ccaagtagag ggccttatct
    43381 tcttgcacga aatcagctat gaaatagcgt aaaaaaactg tgtaactaat atccgtttta
    43441 taacatgaaa tttgctcaga agttacccga caattaataa tttctgagtc aaacttctct
    43501 aagcgcttat ttaattgctt aatccattca tttggaaaat cgctatgaat cagataaaaa
    43561 cgaatcgaac gattatgata acaaatagac ttgattgtcg ttaaaacttg gtctacatag
    43621 ccatagtttg ctgctaggac aatggctttc ttttcacttg attcttgtac cgttaactga
    43681 tttaaaagtc tttttttcat ttcaaactct ttatatattg ctgtgtcaga taaaccacta
    43741 gcttgaccgt tggcgagact gacttccaac atctgacgat aaactgccaa gtgtttctct
    43801 agaggatatc ccatattagc tagtagcgta atacgttcag acatagcatc aactaaggcg
    43861 tgcatccact tttctgtcca aactcttgat agactacctt ttctaatccg ataagcataa
    43921 agacctttat ttaaataaat taccttttcc gataataaat ataccttttg attgaggtaa
    43981 ccatcttctc ctaatttacc catgtcaaag cgcaactgct caaacaatct tgccttatag
    44041 agtttaccac aagcagatat caaagcaaaa ctcttcattt cttgagtttc ataacaagtt
    44101 ctcaaagatg gaaacattgt catatacctt atcataatag gagtctcctg atatatgaaa
    44161 gtagaacatt ccttcacttt cgttgaaaga atagtaattc ccaactgcaa tatcagcttg
    44221 atattctgtt atttttttat atagggtttc tacataatct aactcaatcc aatcatctga
    44281 atccacaaag gtcacataat ttccggacat attattcaga ccggtgtttc gtgcggcaga
    44341 aataccagca ttttcttgtt ctatatagag aattcggtga tccatttctg aaaattcttt
    44401 acaaatttca cctgaagcat ccgtagaacc atcattaacg acaacaatct caatattttt
    44461 atatgtttga gaaataatac tatctagaca tttcctcaga tagttttcta cattgtatac
    44521 tggtacaatg accgtaattt tatcatccac tagcaatttt ctccttgatt tatgtgcgcg
    44581 agttagttta gcctaacttt acaggttgtc ttactcttct tcatcacgtt tacggcgttt
    44641 cgcaaccaat ccaatacctg taacagctgc tagaactcca agtaacacag atccaattga
    44701 cgactcagta cctgtattag gcaattcttt ttgggattta cctgtcgatc cagaagcatt
    44761 tccaacttgt gagttcgaat ggtttgctga atttgaaact gatgtacttg ctgaggctga
    44821 cgcactcgtt gatgctgact cagacgcact ggttgatgcc gattcagacg ctgaggtgct
    44881 tgctgatgct gaggcactgg ttgacgctga agctgaggcg ctagtacttg ctgaagccga
    44941 ggcactggtt gatgctgagg ctgaggcgct agtacttgct gaagctgagg cacttgtcga
    45001 tgctgaggcc gaagcactgg tacttgctga agccgaagca ctcgttgatg ctgaggctga
    45061 agcactggtt gacgctgaag ctgaggcact cgttgaggct gaagccgaag cactcgttga
    45121 tgcggaagct gacgcactgg tacttgctga agctgaggcg cttgtgcttg ctgaggccga
    45181 ggcacttgtt gaggccgatt cagaagctga ggtactcgtt gatgcggatt cagatgctga
    45241 ggtgcttgct gaggccgagg cgctggttga tgcagaagct gaggcactgg ttgacgctga
    45301 agctgaggca ctggttgagg ccgattcaga agctgaggta ctggtgcttg ctgaagctga
    45361 ggcactcgtt gatgccgatt cagacgctga ggtgcttgct gaagctgatg cacttgttga
    45421 tgctgaggct gaggcgctgg ttgatgccga ttcagacgct gagatacttg ctgaggctga
    45481 ggcgcttgta cttgctgaag tcgacgcact ggttgaggcc gattcagatg ctgacgtact
    45541 tgcacttgtt gacgccgatg tgcttgcact tgtcgatgct gacgtgcttg ccgatgtact
    45601 tgtgcttgtt gatgctgacg tgcttgctga agccgaagca cttgtgcttg ctgaagccga
    45661 tgcactcgtt gatgcggaag ctgatgcact tgttgatgcg gaagctgatg cactcgttga
    45721 tgctgaagct gaggcgctag tacttgctga ggctgaggca ctggttgatg ccgattcaga
    45781 tgcactggtt gaggc
//
LOCUS       DAVBCV010000009        45159 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBCV010000009 DAVBCV010000000
VERSION     DAVBCV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.009"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(56..1066)
                     /locus_tag="VQ516_000506"
     CDS             complement(56..1066)
                     /locus_tag="VQ516_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2511000.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VQ516_000507"
     CDS             1215..2384
                     /locus_tag="VQ516_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2511001.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VQ516_000508"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VQ516_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2511002.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VQ516_000509"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VQ516_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2511003.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VQ516_000510"
     CDS             4146..4379
                     /locus_tag="VQ516_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2511004.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            4425..4716
                     /locus_tag="VQ516_000511"
                     /pseudo
     CDS             4425..4716
                     /locus_tag="VQ516_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4919..5149
                     /gene="blpU"
                     /locus_tag="VQ516_000512"
     CDS             4919..5149
                     /gene="blpU"
                     /locus_tag="VQ516_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2511005.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            5864..8017
                     /locus_tag="VQ516_000513"
     CDS             5864..8017
                     /locus_tag="VQ516_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2511006.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            8030..9379
                     /locus_tag="VQ516_000514"
     CDS             8030..9379
                     /locus_tag="VQ516_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2511007.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            9550..10257
                     /locus_tag="VQ516_000515"
     CDS             9550..10257
                     /locus_tag="VQ516_000515"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2511008.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            10459..14184
                     /locus_tag="VQ516_000516"
     CDS             10459..14184
                     /locus_tag="VQ516_000516"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2511009.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVTKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            14277..15719
                     /locus_tag="VQ516_000517"
     CDS             14277..15719
                     /locus_tag="VQ516_000517"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2511010.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            15756..16778
                     /locus_tag="VQ516_000518"
     CDS             15756..16778
                     /locus_tag="VQ516_000518"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2511011.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            16775..17320
                     /gene="purN"
                     /locus_tag="VQ516_000519"
     CDS             16775..17320
                     /gene="purN"
                     /locus_tag="VQ516_000519"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2511012.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIALVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            17404..17913
                     /locus_tag="VQ516_000520"
     CDS             17404..17913
                     /locus_tag="VQ516_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2511013.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            17938..19485
                     /gene="purH"
                     /locus_tag="VQ516_000521"
     CDS             17938..19485
                     /gene="purH"
                     /locus_tag="VQ516_000521"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2511014.1"
                     /translation="MTKRVLISVSDKADIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYDDAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGENKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            19607..20869
                     /gene="purD"
                     /locus_tag="VQ516_000522"
     CDS             19607..20869
                     /gene="purD"
                     /locus_tag="VQ516_000522"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2511015.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFFEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLNYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            21272..21760
                     /gene="purE"
                     /locus_tag="VQ516_000523"
     CDS             21272..21760
                     /gene="purE"
                     /locus_tag="VQ516_000523"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2511016.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            21747..22838
                     /gene="purK"
                     /locus_tag="VQ516_000524"
     CDS             21747..22838
                     /gene="purK"
                     /locus_tag="VQ516_000524"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2511017.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            22848..23075
                     /locus_tag="VQ516_000525"
     CDS             22848..23075
                     /locus_tag="VQ516_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2511018.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            23138..24436
                     /gene="purB"
                     /locus_tag="VQ516_000526"
     CDS             23138..24436
                     /gene="purB"
                     /locus_tag="VQ516_000526"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2511019.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(24491..28429)
                     /gene="strH"
                     /locus_tag="VQ516_000527"
     CDS             complement(24491..28429)
                     /gene="strH"
                     /locus_tag="VQ516_000527"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2511020.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEDDKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGSMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(28744..29460)
                     /locus_tag="VQ516_000528"
     CDS             complement(28744..29460)
                     /locus_tag="VQ516_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2511021.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            29808..31595
                     /locus_tag="VQ516_000529"
     CDS             29808..31595
                     /locus_tag="VQ516_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2511022.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPVYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            31592..32068
                     /locus_tag="VQ516_000530"
     CDS             31592..32068
                     /locus_tag="VQ516_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2511023.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            32096..33001
                     /locus_tag="VQ516_000531"
     CDS             32096..33001
                     /locus_tag="VQ516_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2511024.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            32988..33803
                     /locus_tag="VQ516_000532"
     CDS             32988..33803
                     /locus_tag="VQ516_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2511025.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            33810..34214
                     /locus_tag="VQ516_000533"
     CDS             33810..34214
                     /locus_tag="VQ516_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2511026.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            34513..35679
                     /locus_tag="VQ516_000534"
     CDS             34513..35679
                     /locus_tag="VQ516_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2511027.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            35795..36832
                     /locus_tag="VQ516_000535"
     CDS             35795..36832
                     /locus_tag="VQ516_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2511028.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            37040..37894
                     /locus_tag="VQ516_000536"
     CDS             37040..37894
                     /locus_tag="VQ516_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2511029.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            38044..38301
                     /locus_tag="VQ516_000537"
     CDS             38044..38301
                     /locus_tag="VQ516_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2511030.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(38677..38769)
                     /locus_tag="VQ516_000538"
                     /pseudo
     CDS             complement(38677..38769)
                     /locus_tag="VQ516_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(38805..39101)
                     /locus_tag="VQ516_000539"
     CDS             complement(38805..39101)
                     /locus_tag="VQ516_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2511031.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
     gene            complement(39091..39252)
                     /locus_tag="VQ516_000540"
     CDS             complement(39091..39252)
                     /locus_tag="VQ516_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2511032.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            39322..40086
                     /locus_tag="VQ516_000541"
     CDS             39322..40086
                     /locus_tag="VQ516_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2511033.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            40699..42078
                     /locus_tag="VQ516_000542"
     CDS             40699..42078
                     /locus_tag="VQ516_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2511034.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            42092..42757
                     /locus_tag="VQ516_000543"
     CDS             42092..42757
                     /locus_tag="VQ516_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2511035.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            42871..42966
                     /locus_tag="VQ516_000544"
                     /pseudo
     CDS             42871..42966
                     /locus_tag="VQ516_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            43112..43561
                     /locus_tag="VQ516_000545"
     CDS             43112..43561
                     /locus_tag="VQ516_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2511036.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            43718..44128
                     /locus_tag="VQ516_000546"
     CDS             43718..44128
                     /locus_tag="VQ516_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2511037.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            44244..>45159
                     /gene="pavB"
                     /locus_tag="VQ516_000547"
     CDS             44244..>45159
                     /gene="pavB"
                     /locus_tag="VQ516_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2511038.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAV"
BASE COUNT        12582 a         8714 c        10653 g        13210 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtgttaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaataagga atttagagag ttctttgaga ggggtaaaga
      241 aactactgga cacacctaga gaggtgagta gtgtagtgag gagagaggca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggagaga aagagcgtga tagggataat agccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatcaata acgggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggtta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaattttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcggccttca
     1981 cagcccagtt aatcaagagt caccaatact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaatgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggcagcagg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggactcatgt ctaccttgcc taccgttgat
     3541 ggaaaaggtt ttgacatgct agaccttggt gccaatgcag aaaatacagc ccagcacctc
     3601 catcaatatg cggttctagg ttccttctat gctaaaaatg tccgtggcat tgcgcaacca
     3661 cgcgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgtcagatc
     4081 cgtaccatgc tagaaacaga cgtggttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aggaaattga ccaactccaa agtgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aatgagaaat atcggacaag
     4441 ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat cctcaagcac
     4501 aaacttccac gtaaatccag caaactcaag ccgctaacag ttgaagataa agcctgtaat
     4561 catgcgctat ctaaggagag aagcaaggtt gagaatatct ttgccaaagt aaaaacgttt
     4621 aaaatgtttt caacaaccta tcgaaatcat cgtaaacgct tcggattacg aatgaatttg
     4681 attgctggta ttatcaatca tgaactagga ttctagtttt gcaggaagtc taatagtaaa
     4741 aaagtgatta gaaaacatct tttttaaaaa tagagatgat tttgaaacaa aaaagctaat
     4801 tcaagacgtt tcgatgccaa ttcaagattt ggatgaaaaa aattaataga tactgttata
     4861 ctaaacttgt caagtttgta acaagacaaa tattaaaaat aaaaaagagg tattcgttat
     4921 gaatacaaaa aagatgtcac aatttgaaat tatggatact gagatgcttg cttgcgttga
     4981 aggtggcgga tgcaattggg gagattttgc caaagcaggt gttggaggag gagcagtacg
     5041 aggtcttcag ctaggaatta aaacaagaac atggcaaggt gcagcaactg gtgctgcggg
     5101 aggagctata cttggaggtg tggcctatgc agcgacatgt tggtggtaat tatggatttt
     5161 aaaagtttta ttattggttt agtagttggt atatttggtc cttatatgga tgatttaatt
     5221 agaaaaaaat ttttaaagtc ttcggagaag aaaacagaaa aatctgttaa aaaataatca
     5281 aaactataaa tgatgaatct gaatcaaaat tattttgcgc atgtaaagag gagtcttata
     5341 gtaacgagtc aaaaaaggag taactatgaa tcgtaattta gaacggtgtt atctattctg
     5401 actaggaata gatcatacca gaggtagctt agaaatagca gggacattag aaattgaagt
     5461 aataaatagg atgtcgtaag tgttactatc aatgatttat ttgtttcaag cttgcctagg
     5521 gtgacagtaa aaaatcaatt tcctttcaat agcatatttt tagtgggcag gactcttgtt
     5581 ctgcctattt ttttatccaa aaagtgcagt tgggagggag ataggctcat ttgggaagga
     5641 agtccagttt ttgtttagtg attggggtaa gatagttgtt atcagatgag tttatactct
     5701 tcgaaaatca aattcaaacc acgtcaacgt cgccttgccg tatatatgtg actgacttcg
     5761 tcagtcctat ctacaacctc aaaacagtgt tttgagcagc ctgcggctag tttcctagtt
     5821 tgctctttga ttttcattga gtattaggga aaaggagatg aatatgaaat ttgggaaacg
     5881 tcactatcgt ccgcaagtgg atcagatgga ctgcggtgta gcttcattag ccatggtttt
     5941 tggctactat ggtagttatt attttttggc tcacttgcga gaattggcta agacgaccat
     6001 ggatgggacg acggctttgg gcttggtcaa ggtggcagag gagattggtt ttgagacgcg
     6061 agccattaag gcggatatga cgctttttga cttgccggat ttgacttttc cttttgttgc
     6121 ccatgtgctt aaggaaggga aattgctcca ctactatgtg gtgactgggc aggataagga
     6181 tagcattcat attgccgatc cagatcccgg ggtgaagttg actaaactgc cacgtgagcg
     6241 ttttgaggaa gaatggacag gagtgactct ttttatggca cctagtccag actataagcc
     6301 ttataaggaa caaaaaaatg gtctgctctc ttttatccct atattagtga agcagcgtgg
     6361 cttgattgcc aatatcgttt tggcaacact cttggtaacc gggattaaca ttgtgggttc
     6421 ttattatctg cagtctatca ttgataccta tgtgccagat cagatgcgtt cgacactagg
     6481 gattatttct attgggctag tcatcgtcta catcctccag caaatcttgt cttacgctca
     6541 ggagtatctc ttgcttgttt tggggcaacg cttgtcgatt gacgtgattt tgtcctatat
     6601 caagcatgtt tttcacctcc ctatgtcctt ctttgcgaca cgcaggacag gggagatcgt
     6661 gtctcgtttt acagatgcta acagtatcat cgatgcgctg gcttcgacca tcctttcgat
     6721 tttcctagat gtgtcaacgg ttgtcattat ttcccttgtt ctattttcac aaaataccaa
     6781 tctctttttc atgactttat tggcgcttcc tatctacaca gtgattatct ttgcctttat
     6841 gaagccgttt gaaaagatga atcgggatac catggaagcc aatgcggttc tgtcttcttc
     6901 tatcattgag gacatcaacg gtattgagac tatcaagtcc ttgaccagtg aaagtcagcg
     6961 ttaccaaaaa attgacaagg aatttgtgga ttatctgaag aaatccttta cctatagtcg
     7021 agcagagagt cagcaaaagg ctctgaaaaa ggttgcccat ctcttgctta atgtcggcat
     7081 tctctggatg ggggctgttc tggtcatgga tggcaagatg agtttggggc agttgattac
     7141 ctataatacc ttgctggttt actttaccaa tcctttggaa aatatcatca atctgcaaac
     7201 caagcttcag acagcgcagg ttgccaataa ccgtctaaat gaagtgtatc tagtagcttc
     7261 tgagtttgag gagaagaaaa cagttgagga tttgagcttg atgaagggag atatgacctt
     7321 caagcaggtt cattacaagt atggctatgg tcgagacgtc ttgtcggata tcaatttaac
     7381 cgttccccaa gggtctaagg tggcttttgt ggggatttca gggtcaggta agacgacttt
     7441 ggccaagatg atggttaatt tttacgaccc aagtcaaggg gagattagtc tgggtggtgt
     7501 caatctcaat cagattgata aaaaagccct gcgccagtac atcaactatc tgcctcaaca
     7561 gccctatgtc tttaacggaa cgattttgga gaatcttctt ttgggagcca aggaggggac
     7621 gacacaggaa gatatcttac gggcggtcga attggcagag attcgagagg atatcgagcg
     7681 catgccactg aattatcaga cagaattgac ttcggatggg gcagggattt caggtggtca
     7741 acgtcagaga atcgctttgg cgcgtgctct cttgacagat gcgccggtct tgattttgga
     7801 tgaggcgact agcagtttgg atattttgac agagaagcgg attgtcgata atctcatggc
     7861 tttggacaag accttgattt tcattgctca ccgcttgact attgctgagc ggacagagaa
     7921 ggtggttgtc ttggatcagg gcaagattgt cgaagaagga aagcatgctg atttgcttgc
     7981 acagggaggc ttttacgccc atttggtcaa tagctagaaa gaggagagga tgaaaccaga
     8041 atttttagaa agtgcggagt tttataatcg tcgttaccat aatttttcca gtagtgtgat
     8101 tgtacccatg gcccttctgc tcgtgttttt acttggcttt gcaactgttg cagagaagga
     8161 gatgagtttg tccactagag ctactgtcga acccagtcgt atccttgcaa atatccagtc
     8221 aactagcaac aatcgtattc ttgtcaatca tttggaagaa aataagctgg ttaagaaggg
     8281 ggatctcttg gttcaatacc aagaaggggc agagggtgtc caagcggagt cctatgccag
     8341 tcagttggac atgctaaagg atcaaaaaaa gcaattggag tatctgcaaa agagcctgca
     8401 agaaggggag aaccactttc cagaggagga taagtttggc taccaagcca cctttcgcga
     8461 ctacatcagt caagcaggca gtcttagggc tagtacatcg caacaaaatg agaccatcgc
     8521 gtcccagaat gcagcagcta gccaaaccca agccgaaatc ggcaacctca tcagtcaaac
     8581 agaggctaaa attcgcgatt accagacagc taagtcagct atggaaacag gtgcttcctt
     8641 ggccagtcag aatctagcct actctcttta ccagtcctac aagtctcagg gcgaggaaaa
     8701 tccccaaact aaggttcagg cagttgcaca ggttgaagca cagatttctc agttagaatc
     8761 tagtcttgct acttaccgtg tccagtatgc aggttcaggt acccagcaag cctatgcgtc
     8821 agggttaagc agtcaattgg aatcccttaa atcccaacat ttggcaaagg ttggtcagga
     8881 attgaccctt ctagcccaga aaatcttgga ggcagagtca ggtaagaagg tacagggaaa
     8941 tcttttagac aaggggaaaa ttacggcgag tgaggatggg gtgcttcatc ttaatcctga
     9001 gaccagtgat tctagcatgg ttgcagaagg tgccctacta gcccaacttt atccatcttt
     9061 ggaaagagaa gggaaagcca aactcacagc ctatctaagt tcaaaagatg tagcaagaat
     9121 caaggtcggt gattctgttc gctatactac gactcatgat gccgggaatc aacttttcct
     9181 agattctact attacaagta ttgatgcgac agctactaag actgagaaag ggaatttctt
     9241 taaaatcgag gcggagacta atctaacttc ggagcaggct gaaaaactta ggtacggggt
     9301 ggaaggccgc ttgcagatga ttacgggcaa gaaaagttat ctacgttatt atttggatca
     9361 atttttgaac aaagagtaat gttcgtgttt ttagagttaa ataattttta aactgtgaga
     9421 aagattcttc ttgcagtttt ttctttacaa tttttaaaaa aacatctgct atttattcgg
     9481 ttaaattctt gtgttttttg gttttttgtg gtaaaatgtg ctcaagtaat acgaaaggcg
     9541 aactttaaaa tgtcaaaaca attgatctat tcgggaaaag ctaaagatat ctatacaact
     9601 gaggatgaaa atcttattat ttcaacttac aaggaccagg cgactgcttt caacggtgtc
     9661 aagaaggagc agattgcagg taagggagtc ttgaataatc agatctcatc ttttattttt
     9721 gagaaattaa atgcggctgg tgtggcgact cactttgtgg agaaactttc agacacggaa
     9781 caactcaata aaaaggttaa gattattcct ttggaagtcg tgctccgcaa ctatactgct
     9841 ggttcctttt caaaacgttt tggtgtggat gagggaatcg ccttggagac tccgattgtc
     9901 gaattttact acaaaaatga tgatttggat gatccattta tcaatgatga gcatgtgaaa
     9961 ttcctacaga ttgcgggtga ccagcagatt gcctacttga aggaagaaac gcgtcgtatc
    10021 aatgaactat tgaaagtctg gtttgctgag attgggctta aattgattga ctttaagcta
    10081 gagttcggtt ttgacaagga tggcaagatt atcttggcag acgaattttc accagataac
    10141 tgccgcttgt gggacgctga tggcaaccac atggataagg atgttttccg tagaggattg
    10201 ggagaactaa ccgacgttta tgagattgtt tgggaaaagt tgcaggaatt gaaataatct
    10261 gtttgcaacg gaaaaccttc gtctctcaac taaaaggact caggctgaaa aggtccccca
    10321 gaccttttca ctctgtagag aactaggtga actaacagat gtttacgaaa ttgtctggga
    10381 aaagttgcag ggtttaaaat aacaacctca aggctgtttg ggaatattgc aagagctgaa
    10441 ataaaggaat aagaattgat ggataaacgt atttttgttg aaaaaaaggc tgattttcag
    10501 gtcaagtcag agagtttggt tagagagctc cagcacaact tgggactgtc aagcttgaaa
    10561 agtattcgta ttgtgcaagt atatgatgta tttgacttgg ctgaggactt gtttgcacct
    10621 gcagagaagc acattttctc tgagcaggga accgaccatg ttttagatga agtatctgtg
    10681 caggcggatc ttgctaacta tgctttcttt gccattgaaa gtctgccagg gcagtttgac
    10741 cagcgtgcag cttcgtcaca ggaagccttg cttttgttgg gaagttcgag tgacgtgaca
    10801 gtcaacacag cccaacttta cttggtgaat aaagatattg atgcgactga gttggaagct
    10861 gtcaaaaact acctgctcaa tccagttgat tctcgtttca aggatatcac gacagggatt
    10921 gccaagcagg agttttcaga gtcagacaag accattccaa aattgacttt ctttgaaagc
    10981 tatacagcag aagactttgc tcgctacaag gccgagcaag ggatggccat ggaagtggat
    11041 gatttgctct ttatccaaga ctactttaag tcaatcgggc gcgtgccaac tgagactgaa
    11101 ctcaaggttt tggacactta ctggtctgac cactgccgtc atacgacttt tgagacagag
    11161 ttgaaacaca tcgacttttc agcttctaaa tttcaaaagc aattgcagtc aacctatgac
    11221 aagtatattg ccatgcgcga ggagttaggt cggtctgaaa aaccacaaac cttgatggat
    11281 atggcgacta ttttcggtcg ttatgagcgt gctaacggac gattggatga tatggaagtc
    11341 tctgacgaaa tcaatgcctg ctcagttgaa attgaagtgg atgttgatgg tgtcaaggaa
    11401 ccttggctcc tcatgtttaa aaatgaaacc cacaaccatc caacagaaat tgagccattt
    11461 ggtggagcgg ctacctgtat cggtggagct attcgtgatc cgttgtcagg ccgttcctat
    11521 gtttaccaag ccatgcgtat ttcaggtgct ggtgatatta cagcaccgat ttcggaaact
    11581 cgtgctggga aattgccaca acaagtcatt tctaaaacag cagctcatgg ttattcttca
    11641 tatggtaacc agattgggct tgccacaacc tacgttcgtg aatacttcca cccaggcttt
    11701 gtagctaaac gtatggaact tggtgccgtt gttggtgcgg ctcccaaggg caatgttgtc
    11761 cgtgaaaaac ctgaagcagg tgatgtgatc atccttctcg gaggcaaaac aggtcgtgat
    11821 ggtgtcggtg gtgcgacggg ctcttctaag gttcaaacag ttgagtctgt agagactgct
    11881 ggtgctgagg ttcaaaaagg aaatgccatc gaagaacgca agattcagcg cctcttccgt
    11941 aatggcaatg tcactcgtct gatcaagaag tccaatgact ttggggcagg cggcgtctgt
    12001 gtggctatcg gtgaattggc agacggtctt gaaatcgacc tcaacaaggt gcctcttaaa
    12061 taccagggct tgaatggtac agaaattgcc atctctgaat cacaagaacg gatggcggtc
    12121 gtggttcgtc ctgaagatgt ggataccttc gttgccgaat gtaacaaaga aaatattgat
    12181 gctgttgtgg tggcgacagt aactgaaaaa ccaaatcttg tcatgcactg gaatggtgag
    12241 acaatcgttg acttggagcg tcgtttcctt gacaccaatg gtgtgcgcgt ggttgtcgat
    12301 gccaaagttg tggacaagga tgtcaaactc ccagaagagc gtcaaacatc tgctgaaaca
    12361 ctggaatcag atacccttac ggttctatct gacctcaacc atgcaagtca aaaaggatta
    12421 cagactatct ttgactgctc tgttggacgc tcaacggtta atcacccact tggtggtcgt
    12481 taccaactca caccaactga ggcatctgtg cagaaattgc cagttcaaca cggtgtgact
    12541 catactgcgt cggtcattgc tcaaggtttc aacccatatg tagctgaatg gtctccatac
    12601 cacggtgctg cttatgcggt tatcgaagca actgctcgtt tggtggctgc tggtgccaac
    12661 tggtctaagg ctcgtttctc ttaccaagaa tatttcgagc ggatggacaa gcaagcagag
    12721 cgtttcggtc agccagtagc tgctcttcta ggttctattg aagcacaaat tcagcttggc
    12781 ttgccatcta tcggtggtaa ggactccatg tctggtacct ttgaagaatt gaccgttccg
    12841 ccaaccttgg ttgcctttgg ggtgacgacg gcagatagcc gtaaggtgct ctctccagaa
    12901 tttaaagctg ttggggaaaa tatctactac atcccaggtc aagccctctc tgcagagatt
    12961 gattttgact tgattaagaa aaattttgct cagtttgaag caagccaagc tgaccataaa
    13021 gtgacgtctg catcagctgt caaatacggt ggtgtagttg aaagtttggc tcttgctacc
    13081 tttggaaact atattggtgc agaggtgacc ttgcctgaac ttaaaacagc tttgacagct
    13141 caattaggcg gctttgtctt cacatctcct gaagaaattg ctggagtaga gaagattgga
    13201 caaacgaaag cagactttac actgactgtc aacggtgtga agctagatgg acacaagctt
    13261 gacagtgcat ttcaagggac attggaagaa gtttacccaa cagaatttac ccaagcgaaa
    13321 gaactagaag aagtaccagc tgtggcatca gatgttgtga ttaaagccaa agaaaaggtt
    13381 gaaaaacctg tggtttacat cccagtcttt ccaggaacca actcagaata tgattcagct
    13441 aaggccttcg aaaaagaagg tgcagaggtc aatttggtgc cattcgtgac cttgaatgaa
    13501 gaagctattg tcaagtcagt tgaaactatg gttgacaata tcgacaagac taatattctc
    13561 ttctttgccg gtggattctc ggctgcggat gaaccagatg gttcagctaa gtttatcgtc
    13621 aatatcctgc ttaatgaaaa agtgcgtgtg gctattgata gctttatcgc ccgtggtggc
    13681 ttgattatcg gtatttgtaa tggattccaa gccttagtca aatcgggtct cctaccatac
    13741 ggaaactttg aagatgctaa cagtactagt ccaaccctct tctacaatga tgccaaccaa
    13801 cacgtgacca agatggtgga aactcgcatt gccaatacca actcaccatg gttggctggt
    13861 gtgcaagtgg gcgatatcca cgctattcct gtttcgcacg gtgaagggaa gtttgtcgtg
    13921 acggctgagg aatttgcaga gctccgtgac aatggacaaa ttttcagcca atacgttgac
    13981 tttaacggta aaccaagtat ggattctaag tacaatccga atggttctgt ccatgccatc
    14041 gaaggaatta ccagcaagaa tggtcaaatc atcggtaaga tgggccactc agaacgttat
    14101 gaggatggtc ttttccaaaa tatcccaggc aataaagacc aacacctgtt cgcatcagcg
    14161 gttaaacatt tcactggaaa ataagactta cagattttct aatagatagt atcagtaatg
    14221 taaaagtcat gtaaatctag ctcttgatga ttacaaatga aaattaggta taaaaaatga
    14281 catacgaagt aaaatctctt aatgaagaat gtggtgtttt cggtatttgg ggacatccag
    14341 atgctgctaa gttgacctat tttggactcc acagtcttca acaccgtggt caggaggggg
    14401 caggaatcct ctccaatgat caaggacaac tgaagcgcca tcgtgacatg gggcttttat
    14461 cagaagtttt cagaaatcca gccaatttgg ataaattgac aggagctggt gcgattgggc
    14521 atgtgcgtta tgcgactgct ggcgaagctt ctgttgataa catccagccc ttcctcttcc
    14581 gttttcacga tatgcagttt ggcttggctc ataatggaaa tctgaccaat gcagcctctc
    14641 tcaagaaaga actggaacaa agaggagcaa ttttcagcgc gacttcggac tctgaaatct
    14701 tggctcacct cattcgtcgt agtcataatc ctagcctgat gggcaaaatc aaggaagcgc
    14761 tcagccttgt caaaggtggt tttgcctata tcttgctgtt tgaggacaag ttgattgcgg
    14821 ctcttgaccc taatggcttt cgtccgcttt ctatcgggaa aatggccaac ggagcggtgg
    14881 ttgtttcctc tgaaacctgt gcttttgagg tcattggtgc cgagtggatt cgtgatttga
    14941 agccaggtga gattgtgatc attgatgacg agggcattca gtatgacagc tatacagatg
    15001 atactcagtt ggcgatttgt tctatggagt atatctactt tgctcgccct gattctaata
    15061 tccacggtgt caatgtccat acggcacgta agagaatggg agcgcaattg gcgcgagaat
    15121 ttaagcatga ggcagatatt gtagttggtg tgcccaattc ttccctaagc gcggctatgg
    15181 gatttgcgga agaatcaggc ttaccaaatg aaatgggtct gatcaaaaac caatacaccc
    15241 agcgaacttt tatccaaccg actcaagaat tgcgggagca aggagtgcgg atgaaactgt
    15301 ctgctgtttc gggtgttgtc aaaggcaaac gtgtggtcat ggtggatgat tccattgtac
    15361 gtggaacaac ctctcgtcgt atcgttcagc tcttgaaaga agcgggtgcg actgaggttc
    15421 acgttgccat tggaagtcct gcactagcgt atccatgttt ctacgggatt gatatccaga
    15481 cccgtcagga gctgattgca gccaatcata cggtcgaaga aactcgccaa atcattggtg
    15541 cggacagtct gacttatctt tcaattgatg gcttgattga gtcgattggt atcgaaacag
    15601 atgcgccgaa cggtggtctc tgtgtcgctt actttgacgg tgactaccca acgcctctct
    15661 atgactacga agaagactat cgtagaagtt tggaagaaaa gaccagtttt tacaagtagg
    15721 cgacagattc tccattaaag aaaaggaaaa aacaaatgac aaataaaaat gcatatgccc
    15781 agtcgggtgt ggatgttgaa gcgggttatg aagttgttga acggattaaa aagcacgtgg
    15841 cccgtacgga gcgtgcaggt gtcatgggag ctcttggtgg ctttggtggt atgtttgacc
    15901 tttccaagac tggggttaaa gaacccgtct tgatttcagg gactgacggt gtcggaacca
    15961 agctcatgtt ggctatcaag tacgacaagc acgataccat cgggcaggac tgtgtggcca
    16021 tgtgtgtcaa cgacatcatt gctgcaggtg cggaacccct ctattttctc gactacgtag
    16081 cgacagggaa gaatgaacca gctaagctag aacaagtggt tgctggtgtg gcagaaggtt
    16141 gtgtgcaggc tggtgctgcc ctcatcggtg gggaaacggc tgaaatgccg ggcatgtacg
    16201 gcgaagacga ctatgacttg gctggttttg cggtcggtgt ggctgaaaaa tctcaaatca
    16261 ttgacggttc aaaggtggta gagggagatg ttcttctcgg acttgcttca agtgggattc
    16321 actcaaatgg ttactctttg gttcgtcgtg tctttgcgga ttacacaggt gaggaagtcc
    16381 taccagaatt ggaaggcaag aaacttaagg aagttctact tgagccgact cgtatctatg
    16441 tcaaggctgt cttgccgctc atcaaagaag agttggtcaa cggcattgcc cacatcacag
    16501 gtggtggctt tatcgaaaat gtccctcgta tgtttgcaga tgacctagct gctgaaattg
    16561 atgaaagcaa agttccagtg cttccaattt tcaaagccct tgaaaaatat ggtcagatta
    16621 aacacgaaga aatgtttgaa atcttcaata tgggtgtggg acttatgttg gcggtcagcc
    16681 ctgaaaatgt agagcgtgta aaagaattgt tggatgaagc agtctatgaa attggtcgca
    16741 tcgtcaagaa agaaaacgaa agtgtcatta tcaaatgaaa aaaatagcgg tttttgcctc
    16801 tggtaatggc tcaaattttc aggtgattgc cgaagaattt ccagtggagt ttgtcttttc
    16861 agaccatcgt gatgcctatg tgcttgagcg tgcaaagcag ctcggcgttc tgtcctatgc
    16921 ttttgaactc aaggagtttg agagcaaggc agactacgaa gcagcccttg tcgaactctt
    16981 ggaagaacac cagattgcct tggtttgcct agcaggctac atgaaaatcg ttggaccaac
    17041 cttattgtcg gcttatgaag gtcggattgt caacattcat ccagcctact tgccagaatt
    17101 tccaggagct catgggattg aggatgcttg gaatgctggc gtgggtcagt ctggtgtgac
    17161 cattcactgg gtggattcgg gtgtggatac aggccaggtc atcaaacagg ttcgtgtgcc
    17221 acgactagct gatgatacca ttgacagatt tgaagctcgc atccatgaag cagagtacag
    17281 gttgtatccg gaagtagtga aggctctatt tacagattga ctttttgatg attcatatga
    17341 tatctttgat tttaaattgg agtcagtgtt tgttgaagac ggcttcaaac ggaggtattt
    17401 gtaatgttag aatctaaaaa aacaactcga tatgtatttt atgtctatct gatgttatta
    17461 acttggggaa tcttatttaa gtttgaaaca aatcctgaat ttatagcatt tttcttagct
    17521 ccaaggtata tcaattggat tccattttca gaaccactaa tagtcgatgg aaaaattgtt
    17581 tttgctgaaa tgttatttaa tctgattttc tttattccat taggtgtttg tttccctttg
    17641 ataaaaacta atttatctag tttaagaata gtcgggacag gtttcttgat tagtttattg
    17701 tttgagtgct tacagtatat tttagcaata ggtataacag atataacgga tttgacttta
    17761 aatacgctag gtgtctgtgt aggcttactg atttatcaaa tttttataag agtgttcaaa
    17821 tcacagacta gaaaatggat caatatctta ggtatgctta gccttggttt tgcttatctt
    17881 gttttactgt tactgcattt aattggtgtt taactaatga ttaaaaagga gaatataatg
    17941 actaaacgcg tcttaatcag cgtctcagac aaagcggaca ttgttgaatt tgcccaagaa
    18001 ctcaaaaaac ttggttggga gattatctcg acaggtggaa ctaaggttgc ccttgataat
    18061 gctggggtgg ataccattgc tatcgatgat gtgactggtt tcccagaaat gatggacggt
    18121 cgtgtgaaga ccctccaccc aaatatccac ggagggcttc tcgctcgtcg tgacttggat
    18181 agccacttgg aagcggctaa ggacaacaag attgagctca ttgaccttgt ggtggtcaac
    18241 ctttacccat ttaaggaaac tatccttaaa ccagatgtga cttatgatga tgcagttgaa
    18301 aatatcgata ttggtgggcc atctatgctt cgttcagcag cgaaaaatca tgccagtgtt
    18361 acagttgtgg tagatcctgc tgactacgct gtggttttgg acgaattggc agcaaacggc
    18421 gaaacctctt atgaaactcg ccaacgttta gcagccaaag tatttcgtca cacagcggct
    18481 tatgacgcct tgattgcaga atacttcaca gctcaagtgg gtgaaaacaa gcctgaaaaa
    18541 ctcactttga cttatgacct caagcaacca atgcgttacg gtgagaatcc tcaacaagac
    18601 gcggactttt accagaaagc tttgcctaca gactactcca ttgcttcagc caaacagctc
    18661 aacgggaaag aattgtcatt taataatatc cgtgatgcag atgctgctat ccgtatcatc
    18721 cgtgacttca aagatagtcc aaccgttgtg gctctcaaac acatgaatcc atgtggaatt
    18781 ggtcaagctg atgacatcga gactgcttgg gactacgctt atgagtctga cccagtgtct
    18841 atctttggtg ggattgtcgt cctcaaccgt gaggtggatg ctgcgacagc tgagaagatg
    18901 cacggcgttt tcctcgaaat catcattgca ccaagctata cggatgaagc gctagccatt
    18961 ttgatcaata aaaagaaaaa cttgcgtatc cttgccttgc catttaatgc tcaagaggct
    19021 agcgaagtgg aagcagaata cacaggtgta gtcggtggac ttctcgtgca aaatcaagac
    19081 gtggtcaagg aaagcccagc tgactggcaa gtggtgacta aacgtcagcc aactgagaca
    19141 gaagcgactg ctcttgagtt cgcttggaag gctatcaagt acgtcaaatc aaatggtatt
    19201 atcgtgacca acgaccacat gacacttggt gttggtccag gtcaaaccaa ccgtgtggct
    19261 tctgttcgcc ttgccattga ccaagccaaa gatcgtctgg acggggcggt ccttgcttca
    19321 gatgccttct tcccatttgc ggataacgtg gaagaaatcg ccaaagcagg aattaaggcc
    19381 atcatccagc ctggtggctc tgtccgtgac caagaatcca tcgaagcagc ggataaatac
    19441 ggcttgacta tggtctttac aggtgtgaga cattttagac attaagaaga taaaagggaa
    19501 gaaaacagtt tctttccttt tttggcttaa aatgctaact gaaacaagat taaaacgaac
    19561 ttttttgata taatgttggt aaataattcg caaaagaggt tgaggaatga aactgcttgt
    19621 tgtcggttct ggtggtcgtg agcatgcgat tgctaaaaag ttacttgaat caaaagacgt
    19681 ggaaaaagtc tttgtagctc ctgggaatga tgggatgact ctggatggtt tggaattggt
    19741 aaatatctct atttccgaac attctaaatt gattgacttc gcaaagacca atgatgttgc
    19801 ttggaccttt atcggtccag atgatgccct tgctgctggt atcgtggatg attttaacca
    19861 agctgggctt aaggcctttg gtccgactag ggctgcagcg gagctggagt ggtccaagga
    19921 ttttgccaag gaaatcatgg tcaaatatgg tgtttcgaca gcagcctatg gcacattttc
    19981 agatttcgag gaagcaaagg cctatatcga aaagcatggt gcgcctatcg tagtcaaggc
    20041 ggatggcttg gcgcttggga agggtgtcgt cgtttctgag acggttgagc aagcggtcga
    20101 agccgctcat gagatgcttt tggacaataa atttggtgac tcaggtgcgc gcgtggttat
    20161 tgaggaattc tttgaaggag aggaattttc actctttgcc tttgtcaatg gtgataagtt
    20221 ctacatcatg ccaacggctc aggaccacaa acgtgcctat gatggcgaca aagggcctaa
    20281 cacgggtggt atgggtgcct atgcgccagt tccacactta ccacagagtg tagttgatac
    20341 agcggttgac accattgtca agccagttct agaagggatg attaaagaag gtcgccctta
    20401 tctgggagtt ctttacgcag ggcttatcct gacagctgat ggaccgaaag tcattgagtt
    20461 caacgctcgg ttcggagatc cagaaactca gattatcttg cctcgcttaa cctctgactt
    20521 tgctcaaaat atcacagata tcctggatag caaggagcca aatatcacgt ggacggacaa
    20581 gggtgtgact ctgggtgtgg ttgtcgcatc caagggctac ccgctaaatt attcaaaggg
    20641 tgttgagttg ccagtcaaaa ccgatggcga catcattacc tactatgcag gggctaagtt
    20701 tgcggaaaat agcagagcac tgctctcaaa cggcggacga gtttatatgc tcgttaccac
    20761 agcagatacc gtcaaagaag cccaagccag catctaccaa gaactatccc aacaaaaaat
    20821 agaaggactc ttctaccgaa cagatatcgg aagcaaggca attaagtaaa gatataagaa
    20881 taacgcgccg tagtcgccaa acacgataat ggtcgtcgtg gtgaaaagac cagaacagtg
    20941 aatgttctgg tcagggggaa acttggagac cgtaggctca aagtttagga atgaaaccga
    21001 aggtttgctt ccgcctccat cacctaagac cattatcaaa aagaaaaata aaaattcaca
    21061 aaatacgtta atgatcgtat ggtttgcgag cgttagcgag ctaatataga acaatcaccg
    21121 ccgttgtgaa agaacgattg gatgataatc caatcgttca gggaaattgg aagaccttgg
    21181 gtttccaatt taggcatgag acacctttgg tggctgctgc cgtccctcac aagctaaggt
    21241 gattgttgaa aaagaggaaa aaggagaaga aatgaaacca gtaatttcca tcatcatggg
    21301 ctcaaaatcc gactgggcaa ccatgcaaaa aacagcagaa gtcctagacc gcttcggtgt
    21361 agcctacgaa aagaaagttg tttccgcaca ccgcacacca gacctcatgt tcaaacatgc
    21421 agaagaagcc cgtagtcgtg gcatcaagat catcatcgca ggtgctggtg gcgcagcgca
    21481 tttgccaggc atggtagctg ccaaaacaac ccttccagtc attggtgtgc cagtcaagtc
    21541 tcgtgctctt agtggagtgg attcactcta ttctatcgtt cagatgccgg gtggggtgcc
    21601 tgttgcgacc atggctatcg gtgaagctgg agcgactaac gcagctctct ttgccctccg
    21661 tctcctctct gtagaagata agtccattgc ggatgcactt gccaactttg ctgaagaaca
    21721 aggaaaaatc gcagaggagt cgtcaaatga gctcatctaa aacaatcgga attatcggtg
    21781 gcggtcaact gggtcagatg atggccattt ctgctatcta catgggccac aaggttatcg
    21841 cgctggatcc tgcggcggat tgcccggcct ctcgcgtggc ggaaatcatt gtggcacctt
    21901 ataacgatgt ggacgccctc cgtcagttgg cagaccgttg cgatgtcctc acttatgagt
    21961 ttgaaaatgt cgacgctgac ggtttggatg ccgttatcaa ggatggacaa ctccctcaag
    22021 gaacagatct gctccgcatt tcgcaaaatc gtatttttga aaaggacttt ttgtcaaaca
    22081 aggctcaagt cactgtggca ccctacaagg tcgtgacttc tagcctagac ttggcagata
    22141 tcgacttgtc gaaaaactat gtcctcaaga ctgcgactgg tggctacgat ggtcatggac
    22201 aaaaggttat tcgttcagaa gcagacttgg aagcagccta tgcgctagca gactcagcag
    22261 actgcgtctt ggaagaattt gtcaactttg accttgagat ttctgtcatc gtgtcaggaa
    22321 atggcaagga ggtgacgttt ttcccagttc aggaaaatat ccaccgcaac aatatcctgt
    22381 ctaagaccat cgtaccagcc cgcatttctg aaagtctagc agacaaggct aaagctatgg
    22441 cagtgcgaat cgcagaacaa ctcaacttgt ctggaactct ctgtgtggaa atgtttgcga
    22501 ctgatgatga cgttattgtc aatgaaatcg ccccacgacc acataactct gggcactatt
    22561 ctattgaagc ctgtgatttc tctcagtttg acacccatat tctgggtgtt ctcggagcac
    22621 cattaccagt catcaaactc catgcgccag ccgttatgct taatgtcctc ggtcagcatg
    22681 tcgaggctgc tgagcaatat gtgacagaaa attcaagcgc ccacctccac atgtatggta
    22741 aaatagaagc gaagcacaac cgcaagatgg gacatgtgac cttgtttagt gatgtaccgg
    22801 atagtgtgga tgagcttggg gaagggattg atttttagga caagtctatg atacaaatta
    22861 tcgttaatac atttattgaa aaggataaga ctggagcagt tgttgaagtg ttgtatgcca
    22921 gtgctgacca agataaggta caagctaaat atgaagaact agctgcacaa taccccgaaa
    22981 attatttagc tatctatgat gtaccgctgg atacggattt gaatacacta gatcattacc
    23041 cgtctgtgtt tattgaaaaa gaggagtttg agtagaaatc ttggtttacc tagatagctt
    23101 attcccaaca gcttaagaag aaaggaaaaa ttaacacatg atcaaccgtt actctcgccc
    23161 tgagatggcg aatatttgga gtgaagaaaa taaataccgt gcttggcttg aggtggaaat
    23221 cctctctgac gaggcatggg ctgagttggg ggaaatccct aaggaagatg tggctttgat
    23281 tcgcaagaag gcggactttg acatcgaccg tattttggaa attgagcagg agacgcgcca
    23341 cgatgtggtg gctttcacgc gtgcggtttc tgagactctt ggtgaagagc gcaagtgggt
    23401 tcactatggg ttaacttcta ccgacgtggt ggatactgct tatggttacc tctacaagca
    23461 ggccaacgac atcatccgtc gtgaccttga aaacttcact aatatcatcg ctgacaaggc
    23521 caaggagcac aagttcacca tcatgatggg gcgtactcat ggtgtgcacg ctgagccgac
    23581 aacctttggt cttaaattgg caacttggta cagcgaaatg aaacgcaata tcgagcgttt
    23641 cgagcatgca gctgctggtg tagaagctgg taagatttct ggtgcggttg ggaactttgc
    23701 caatatccca ccatttgtag aggagtatgt ctgcgataaa cttggcatcc gtgcccaaga
    23761 aatctctaca caagtccttc ctcgtgacct tcacgctgag tactttgcgg ttcttgccag
    23821 cattgcgact tcaatcgaac gtatggcgac tgagattcgt ggtctacaaa aatctgagca
    23881 acgcgaagta gaagagttct ttgctaaagg gcaaaaaggg tcttcagcaa tgcctcacaa
    23941 acgcaaccca atcggttctg aaaatatgac tggtctggcg cgtgtcattc gtggtcacat
    24001 gattacggct tatgaaaacg tcgctctctg gcacgaacgc gatatttctc actcatcagc
    24061 tgagcgtatc atcacaccag atacgaccat tttgattgac tacatgctca accgttttgg
    24121 aaatatcgtc aagaacttga cagtcttccc agaaaatatg atccgaaaca tgaactcgac
    24181 ttttggtctt atctttagcc aacgggctat gttgacattg attgaaaaag gcatgacccg
    24241 tgagcaagcc tatgacttgg tgcaaccaaa aacagcctac tcttgggaca accaagtaga
    24301 ctttaaacca cttcttgagg cagattcaga agtaacatca cgtctcactc aagaagaaat
    24361 cgatgaaatc ttcaacccag tttattacac caaacgagtg gatgatatct ttgaacgtct
    24421 tggactaggt gattaattaa aaaataaaca gcgagcttca atctcgctgt ttatttttta
    24481 tcgaaaagac ttagtcttct tttcttttag tgagtccata ggctgctagt gtggccatga
    24541 gtcctgcgac tactagtcct gcagaatcgt gagttcctgt ttcaggaagt tttttctcta
    24601 ttaccacagg agctggatct tgaggaagag ctttgctttc ctcagcagga gcagttgatg
    24661 gagctggttg gcttgggatt tctagttttg gtttttcttc agcaatagcg gcttgtccgt
    24721 tttcatcgcc tacatgtgtt accatagttc cgacttcgac tatttgagta acggcttcct
    24781 gtgctacgac actatttaca agtgttttca cttccttacc atcggcagaa gtgctcacag
    24841 agtagaagtt gctacgatgt ccattgacgc ccttagtaat gacttgtgtt tttcctttga
    24901 gtaagagtgg attttcacga gtcactgtgg taaatggaat ttcttctttt tggatatcca
    24961 gtctaggttt tacctcagta gttggtgcaa gaccactttc atcacccttg tgagttacag
    25021 gagcgccaac ttcaaccact tggtttataa cttctttggt tacctggcta tcaaggactg
    25081 tttctgttgt ttttccattt tcagtgagta cagagatgta atgagttcgc tcacctttga
    25141 ctcctgctgt gataatattt tcctgaccag ctgggaggtt aggattttct ttcttgataa
    25201 cttcaaatgg aatttcttca gttcttgtga tgagttctgg tctggtttca acattggcag
    25261 ccacttcatt ttcatccagg cttcctgaat gagttgcagc tggtttgagg ccttgaagag
    25321 cggcttttag gttggctaca agtgtgtcaa gctcagcttg tttattacgg ttgaggttgt
    25381 aatttagagc tgttttagct gcgtcaaggg cctcaagact ttctttacta tatccttcta
    25441 agtttgtagg aattttagct aattcttcgc ggagagcatt ataattagca cggaagtagt
    25501 ctttgttgtg gtctgcaaag gcagtcatga gttcaaagat ttcctcttcc ttgtattcag
    25561 cgcttggtct atctgcccag attgaaagca tacttccgac tgttggaaga tctacttcag
    25621 gatatttggt agaagctagt tgattgaatg gtgtttttcc agtattctca atagctttct
    25681 tgaggaaacc accaccatct tctggttttt gaccaagaat gtagtaccag tcaccgttgg
    25741 tattcaagaa tttatagcct ttgcttgcta ggtattgagg tgatgcgagg ttatatcccc
    25801 accagccttt agaccagtaa gaaatcaaga catctttgtc aaactgaaca tcgtccttgt
    25861 cttcatagta gaagccatcg ttgaaggcca ttggttgaag ccctctttct ttggccatag
    25921 ctgcgagggt gttggcatat tcggcaaatt tgccatagag ttgataccac ttgaggtagt
    25981 accagccttg ggcactagtc gcatcgttgg cgtattcgtc agtaccaaag ttgaaaatct
    26041 ttgttttacc tgcaaagaag tccatgtatt taccgatgag ggcttttaca aagttcatcg
    26101 cttcttcgtt tttcaagtcc atagttgttt ttgaaacttt atcaaagtgg gcttgaggat
    26161 ttttaatacc taatttttcc atggcaacca gcatagcatc catgtgacct ggactgttaa
    26221 tagctgggat gagaccgatg tccttagatt tagcgtattc aattagctct gttacttctg
    26281 cctgtgttag tgcagtaccg tttggatcgt cgtagtaagc tttagttcct tcgataatag
    26341 cttttttaac gtcatcacta gcataggttt ttccgttggc agtaatggtc atatcatcga
    26401 gtagaaagcg aagtccgtca tttcctagaa ggagatggac atcagaatat ccgagctcac
    26461 tggccttgtc tacgatgcgt ttgagctggt tcagagtaaa gtatttgcgt ccagcatcga
    26521 ttgagattac cttgtttttg gcaagttttt caacctcacg tttagcttct tcttcttttt
    26581 gagcttcagg cgtgagggtc aagttgttga cagtttcttg aagtttagca atggcttgat
    26641 caatcgtatc ttgttgggca cggctaaggt tgctatcgag agagcgaata gctttttcag
    26701 cttcttttac ggccgcgacg ctttctgcag tataacggtt caggtctttt ggtacctcgt
    26761 taagtgtttg ctctgcagat tcataatcag ctgcgaagta ttcagcgttg gcatttgcaa
    26821 aatgacgcat gagtttgaag aggcgtgatg gtgaataacg tgcagatgga gtgtcagccc
    26881 aagcagctac catactaccg atgattggga tatcagctcc ttctgttttt ggtacagaag
    26941 tgattggtgt gtttttaata ccattgagcc cctgatcgag attgtaccag ccttggccat
    27001 cagcgtttcg tccaagaacg tagtaccaag catcattggt attaaggatt tggtgacctt
    27061 tttcagctag tagtttagaa gaagcgacat cgtagcctcc ccaaccacca gtccacatag
    27121 aaacgatgat gtctttgtca aaactaccaa agcttgtgtc gctattgtag tagataccgt
    27181 cgttaaaggc cattggtttg agaccgtgcg attttacaat acgagcgagg tcattggcgt
    27241 aggcaataaa tttttcatag ccttttacag ggtagccttc gtttggatag tatttatcag
    27301 cttgaagcac actccaacct ttagcatctg tcgcatcatt ggcatattca tcaagtccga
    27361 tgttgaagat ttcagtcttt ttcgcgaaat aagcagcata cttgtcgata agggcttttg
    27421 taaaagcgac agcttgttcg ttgtcaagat tgacagtacg ggctgattcc ttcccaaaat
    27481 agctaaagtt agggttttgg attcccaatt ctttcatggc attgagaatc gcatccatgt
    27541 gaccaggact atttactgtc ggaatgagac cgataccttt atctttggca tagttaatca
    27601 gatctgtcat ttgactttct gttaagtgat tgccgtttgg atcgttgtaa taatcatttg
    27661 tacctttttc aatggcgcgt ttgacatcgt cactggcata ggtcttgccg ttagctgtga
    27721 tgctcatatc gtccaacatg aaacggagtc catcatttcc gactaatagg tgtaaatcag
    27781 tgtagccata atgtttcgct ttatcgatga tttccttgag ctgttctggt gagaaatatt
    27841 tacgtccagc atcaatagaa acaattttct ttttcgctag tttttcattt acagttgcag
    27901 cacgttcctt tcctgcctct gtttccggtt tgtcatcctc tgctttcgct tcatcttttt
    27961 tagctggttt atccttgtca gtcttgtctg tatttgactc tttagaatca acctctttcg
    28021 cttcttcctt tttagggcta gcttcttctg cctttttatt agcagtttct ttttcagcag
    28081 aagttggagt taccacttct gctttatcac taggagttga actaacttcc tcttgtggtt
    28141 tttcttctgt ttttggaaga ctagctacct tatcagtagc tggagtttct gtttctacag
    28201 tttttggagc ttctggttga agctctgctt taggtgtttc ctcagtccga ttttcggatg
    28261 attgagggga atcagaaacc gtatggatgg tcggttggtt ttctgtagta gtaggagtaa
    28321 ctccatcggc tgcaacagtc tgtgcttgga aggcaaatcc aattagaaca gaagctgctc
    28381 ctacagcgta tttacgaata gaaaaacgct gttgtttttc atgtttcatt gcaaaacctc
    28441 ctgattgcat tgttatattg atagcgatta tataaatcaa cgcctttatt ttatttctta
    28501 tattaacgag agtcaagagg agatgacaaa aaactataat aagtataaaa aaatataaaa
    28561 tttaaactta agatttcaga ttggtcggaa aaaatacgta tatatatcta gtataatttt
    28621 tggttctatt tctataaaat attccacaaa ttatagaatt ttccaaaaat aggtaagcgc
    28681 tacctttttg gtgtggtata ataagcatag aaaaagccca agcgattagc tcaggctttc
    28741 ttcttagtga tcacggtcac atgagataaa tttaatcttg tagtaatcag atcgtttgta
    28801 agtttcactg tattctaaaa cttggccagt tgattcgagt ttggtgattt tagtttgtag
    28861 gacagtaggg aattgttcat cgactccgag gactgaagcc gcatgttctg gagttggaaa
    28921 gactatttcg ttgatttctt caaagtgttc atcattcatg tgaatgtggt agtctaactt
    28981 gaaacgatta tagatagaac tatagtattc aaggtttgga taatttgcgt tgatatattg
    29041 ttctgggatg taggatgtat ggtagatata aacgacaccg tttgattcgc ggatacgttc
    29101 aatcttgtag tagaattgat cgccgcgtag acccaatttt tccaagtaaa caagcttgtt
    29161 tccgcgttca attgaaagaa cagttacctt atcatcttta gcattgaaga gttcaatatc
    29221 tgaaaactct acaagcttgt gtttgcgtgc acgtgaaacg aaggttcctt ttccttgttg
    29281 gcggacaata tagccatctt tggcaaggtc gtttaaggcg cgaacaactg tgatagagct
    29341 gacatcgtac attgaaatga gttctgcttc agtgtaaaat ttatctccac tgctaaactg
    29401 cccagagatg attttatttt ttaattcgtc ttttatgtat tgatatttgg gaattgccat
    29461 atttttacct ctattttcct tctccatagg aaattttacc ataaaagcga aaaaaatagt
    29521 agtcttttga ataaaaaatt agaataatag tttgaaaaat taagcgatat agggattgct
    29581 catattttat caatgtattc tatacaaaat tattttaggt cccatattcc gaatttaaag
    29641 agcgtttgaa atgggtctaa aaatgtttga aactaattga atgtgtgtat aatagtaaaa
    29701 atgcagtaaa tatcccgaca atttctattg acaattcaaa cagattggtt tataattaat
    29761 ataacaacaa atgaaagcgc aaactttcgc ggtcggaagg tagttttatg acacgatttg
    29821 agatacgaga tgatttctat ctcgatggaa aatcatttaa gattttatct ggtgccattc
    29881 attattttag ggttcctcca gaggattggt atcattcgct ctataacttg aaggctcttg
    29941 gttttaatac ggtagagact tatgttgctt ggaatttaca cgagcctcgt gaaggtgagt
    30001 ttcattttga aggtgatctg gatttagaga aatttctcca aatagcgcag gatttgggtc
    30061 tctacgcaat tgtgcgtccg tctccattta tctgtgcgga atgggaattc ggtggcttac
    30121 cagcttggct cttgaccaag aacatgcgaa ttcgctcatc cgacccagta tatatcgagg
    30181 cagttggtcg ctactatgat cagttattgc caagactggt gcctcgtttg ttggacaatg
    30241 gtggcaatat tctcatgatg caggttgaaa atgagtatgg ttcttacgga gaagataagg
    30301 cttacctgag agcgattcga cagctaatgg aagagtgtgg cgtaacctgt cccctcttta
    30361 catcagatgg tccatggcga gctactctga aagctggaac cttaattgaa gaggacctct
    30421 ttgtaacagg aaactttggt tctaaggcac cttacaactt ttcgcagatg caggaattct
    30481 ttgatgaaca tggtaagaaa tggccactca tgtgtatgga gttctgggat ggttggttca
    30541 atcgctggaa agaaccgatt atcacacggg atcctaagga attggcagat gcagttcgag
    30601 aggttttgga acaaggctct atcaatcttt acatgttcca cggtggtaca aactttggtt
    30661 tcatgaatgg ttgctcagct cgaggaactt tggacctgcc acaagttacg tcttatgatt
    30721 acgatgccct tctggatgaa gaaggaaatc caactgctaa atatcttgca gtcaagaaga
    30781 tgatggcaac acatttttca gagtatccgc agttggaacc actctacaaa gagagtatgg
    30841 agttggatgc tattccacta gttgaaaaag tttctttgtt tgaaacctta gatagcttgt
    30901 caagtcctgt agaaagtctc tatcctcaaa agatggagga gctgggacaa agttatggct
    30961 acctacttta tcgaacagaa acaaactggg atgcagaaga agaaagactt cgtatcattg
    31021 atggtcgaga tagggcccag ctgtatgtcg atggtcagtg ggttaaaact caatatcaga
    31081 cagagattgg ggaagatatt ttttatcaag gtaaaaagaa agggctatct aggttagata
    31141 tcttgataga aaatatgggg cgtgtcaact atgggcataa gttcttagcg gatacgcaac
    31201 gtaagggaat tcggacaggg gtctgtaagg atctgcattt cttactaaac tggaaacact
    31261 atccactccc actagacaat cctgagaaaa ttgatttttc aaaaggatgg actcaaggac
    31321 aaccagcctt ttacgcttat gactttacag tcgaagagcc aaaagatact tacctagact
    31381 tgtctgagtt tggtaagggg gttgcctttg tcaatgggca gaatctagga cgtttttgga
    31441 acgttggccc aactctctca ctttatatcc ctcatagcta tctcaaggaa ggtgccaacc
    31501 gcatcattat ctttgaaaca gaaggtcaat ataaagaaga gattcattta actcgtaaac
    31561 ctacactaaa acatataaag ggggaaaact tatgacaatt gtaggatgcc gtattgatgg
    31621 acgtttgatc cacggacaag tagccaatct ttgggctgga aaactaaatg tttcacgcat
    31681 tatggttgta gacgacgaag ttgtcaacaa cgatattgaa aagagtggtt tgaaacttgc
    31741 gacaccacca ggtgtgaaat tgagtatttt gccagttgag aaagctgcag ccaatattct
    31801 tgctggtaaa tacgatagcc aacgtctctt tatcgtggct cgtaaaccag accgcttcct
    31861 tggtttggta gaagcaggtg taccacttga aacccttaat gttgggaata tgtctcaaac
    31921 accagaaaca cgttctatta cacgttctat caacgtagta gacaaggatg tggaagactt
    31981 ccacaaactg gcagaaaaag gtgttaaact tactgctcag atggttccaa atgatccaat
    32041 ttcagacttt ttgagcttat taaaatagga aaaaaatttt taggaggtca ttgttatgat
    32101 acaatggtgg caaattttac ttctcacttt gtactcagct tatcaaatct gtgatgagtt
    32161 gacgatcgtt tcatctgcag gttctcctgt atttgctggt ttcattactg gtttaatcat
    32221 gggagatgtg actactggtt tacttatcgg tggtaacttg caactgttcg ttcttggggt
    32281 tggtaccttc ggtggtgctt ctcgtatcga cgcaacttct ggtgcggttc ttgcgacagc
    32341 cttctctgtt tcacaaggaa ttgatgcacc gcttgccatt actacaatcg ctgtaccagt
    32401 agcagctctc ttgacttact tcgacgttct tggtcgtatg actactacct tcttcgctca
    32461 ccgtgtggat gctgcaatcg aacgctttga ctataaaggt attgaacgca actacttgct
    32521 tggtgcgatt ccgtgggctc tatctcgtgc ccttccagtc ttctttgccc ttgcttttgg
    32581 tggtgccttt gtacaatcag tagtagactt cgttgaagcc tacaaatggg ttgcagatgg
    32641 cttgacactc gcaggacgta tgcttccagg tcttggattt gcaatcttgc ttcgttacct
    32701 tccagttaaa cgtaaccttc actaccttgc tatgggattt ggtttgacag ctatgttgac
    32761 tgttctttac tcatatgtaa caggtcttgg tggcgctgtt gctggtatcg taggtactct
    32821 tcctgctgaa gttgctgaaa aaattggttt cgtgaacaac ttcaaaggtt tgtctatgat
    32881 tggtatttct atcgtaggta ttttccttgc agtgcttcac ttcaaaaata gccaaaaagt
    32941 agctgtagca gcaccttcta caccatcaga aagtggggaa atcgaagatg acgaattcta
    33001 attacaaact tacaaaagaa gattttaatc aaatcaacaa acgtagcttg tttactttcc
    33061 aattaggttg gaactacgaa cgtatgcaag cttctggtta cctttacatg atcttgcctc
    33121 agttgcgtaa aatgtatggt gatggaactc ctgaattgaa agaaatgatg aaagttcata
    33181 ctcaattctt caatacttca ccattcttcc ataccattat cgctggtttt gaccttgcca
    33241 tggaagaaaa agatggtgta ggttcaaaag acgctgttaa cggtatcaag acaggtttga
    33301 tgggaccatt cgctcctctt ggggatacaa tctttggttc acttgtacct gctatcatgg
    33361 ggtcagtcgc agcaactatg gctatcgctg gccaaccttg ggggatcttc ctttggattg
    33421 cagttgcagt agcgtatgac atcttccgtt ggaaacagtt ggaatttgct tacaaagaag
    33481 gggttaacct tatcaacaac atgcaaagta ccttgacagc tttgattgac gctgcatctg
    33541 tacttggtgt cttcatgatg ggtgctcttg tagcaacagt gattaacttt gaaatttctt
    33601 acaagttgcc aatcggtgaa aagatgattg atttccaaga catcttgaac caaatcttcc
    33661 cacgtttgct tccagcaatc tttactgcct ttatcttctg gttgcttggt aaaaaaggta
    33721 tgaactctac taaagctatc ggtattatta tcgtacttgc tttggctctt tctgcccttg
    33781 gtcactttgc acttggaatg taattcctta tgactaaatc attaattttg gtgagccatg
    33841 gtcgcttctg tgaggagctt agaggtagca cagaaatgat tatgggccca caagacaaca
    33901 tttacacagt agctcttctt ccagaagatg gcccagaaga atttactgct aaatttgaag
    33961 ctgttattga aggattggat gatttcctag tctttgcgga tcttctcggt gggacacctt
    34021 gtaatgtggt gagtcgcttg atcatggaag gtcgtgatat tgacctttac gcagggatga
    34081 atcttccaat ggtgattgaa tttatcaatg cgagccttac aggcgcagat gcggactaca
    34141 agagccgtgc tgcagaaagc attgtgaaag ttaatgacct gttagcgggc ttcgatgatg
    34201 acgaagatga ataatactct tcgaaaatct cttcaaacta cgtcaacgtc gccttgccgt
    34261 aggtatatgt tactgacttc gtcagtctta tccggcaacc tcaaaacggt gttttgagct
    34321 gacttcgtca gtcttatccg gcaacctcaa agcagtgctt tgagcagcct gcggctagtt
    34381 tcctacagat tttagttgga actcgattca attcatgtga caacgtgaaa atcgttagag
    34441 cattttatat agaatataca tgggaatgga gcttactccc attcccatat ttaatagaaa
    34501 aagaggaact caatgctaca ttatacaaaa gaagacttgc tcgaattggg tgcagaaatc
    34561 actacgcgtg aaatctacca acagcctgat gtatggagag aagcttttga attttatcaa
    34621 gcaaaacgtg aagaaattgc agcctttcta caagaaatcg ctgataaaca tgactatatt
    34681 aaggttatct tgacaggtgc tgggacttct gcttatgtgg gagatacctt gctaccttac
    34741 tttaaggaag tctatgacga acgcaaatgg aatttcaatg ctattgcgac aacagatatc
    34801 gttgccaaac cagcaaccta tttgaaaaaa gatgtggcaa ctgtccttgt gtcttttgct
    34861 cgtagtggga actcgcctga aagtgtggcg actgttgatt tggccaaatc cttggtggat
    34921 gagctttatc aagtgacgat tacttgtgca gcagatggta aattggctct tcaagctcac
    34981 ggtgatgacc gtaatctctt gctcttgcaa ccagctgtct ctaatgatgc tggatttgcc
    35041 atgacttcta gctttacgtc tatgatgttg acaactctct tggtctttga tcctacagaa
    35101 tttgctgtta agtctgaacg ttttgaagtt gtatctagtc ttgcccgtaa agttttagac
    35161 aaggcagaag atgtcaaaga gctcgttgat ttagacttta accgtgtcat ctatctaggc
    35221 gctggtcctt tctttggact tgctcatgaa gctcagctca agattttgga attaactgct
    35281 ggtcaagttg cgaccatgta tgaaagccca gttggcttcc gtcacggtcc aaaatctctt
    35341 atcaacgaca atacagttgt tttggtcttt ggtacaacga cggactacac tcgtaagtac
    35401 gacttggact tggttcgtga agttgctggt gaccagattg ctcgtcgtgt tgtgcttttg
    35461 agtgatcaag cttttggtct tgaaaatgtc aaagaagtgg tccttggttg tggcggtgtc
    35521 ttgaatgata tttaccgtgt cttcccttac atcgtttatg cccaactctt tgccctattg
    35581 acttcactca aggtagaaaa taaaccagat acaccgtctc ctacaggtac agtaaaccgt
    35641 gtagtacaag gtgtcataat tcacgaatat caaaagtaag acagtgttta tgaattcttg
    35701 acaagaggat ttgtaaatta tcagataaac catagattgt cagtacgctt tctatggttt
    35761 gtttgcttga gagaaatagt aaaaggagaa cagaatgaaa gcatacacag agcgtgtatt
    35821 tggaaatgtt gagggtgagg atgtcttggc ctatcgattt gagacagacg gtggctacca
    35881 acttgaggtt atgacttatg gtgcgactat cttgcgctat gtcgcacctg acaaggctgg
    35941 aaattttgcc aatgttatct tgggatttga tgactttgat agttatgtag gcaatagtcc
    36001 caagcatgga gcaagtgtag gtcctgtagc gggtcgtatt gcaggtgcga cctttgagct
    36061 caatggtaag acctatgacc ttgaggttaa taatgctagc aactgtaatc acagtggttc
    36121 aactggttgg gattccagct tgtttgaagt tgaagaagta agcgatcatg gcttgactct
    36181 ctacacagag cgtacagatg ggacaggagg gttccctgga aatctcaaga tttggatcag
    36241 ttatcacttg gaagaaactg gtgcctatga aatcagctac aaggtaacga ccgatcagga
    36301 tacgctggtc aatccaacca accacagcta tttcaacttg tctggtgatt tcacgcagac
    36361 gattgaccgt catgtcttcc aactaaacac agagggcatt tactcaatcg ctcctgacgg
    36421 tgttcctgcc aaaactccag aagccaaccg tgatgtggtc aaacacatct acaatggtgc
    36481 cttgttgaag gatatctttg cagaagaaga tgagcaaatc cagctggcat caggtttgga
    36541 tcatccattt gcccttcctg caggccatga caatgctgga ttcctttatg accaaaattc
    36601 aggtcgcttc ctgcttttca agacagaagc tccttgcttt gtggtctaca cagcaaactt
    36661 tgtggatgaa agtgtcatca taggaggtca gccaatgcta cagcacaatg ggattgctct
    36721 tgaagcgcaa gctttaccag atgccattca cagtgacctt aaaggccaag tcattcttaa
    36781 agctggtcaa accttcacca gtaagacacg ttatgaactt gttgtgaaat aaaaaaagct
    36841 ggtcaaaaag tctttaaaat taaaataacg catattgtca ggtgatgaaa agtttgataa
    36901 tatgcgtttt attatgaaat gatttacttt atattatcct gaaatggtgt tatttttgag
    36961 ttatctggct tgactttctt gatggaaatt atataatagt tgtaacaatg ataattacaa
    37021 ctagaaaaga ggaccattta tgacctatga atacaagagt cacatttatt tggcagagac
    37081 agctttaaat gtaaaggatt tggccagtca aacagccttt tatcagcaag ttattggttt
    37141 agaaatctta tctcaaactg agacagagtc cattctaggc cttggtggaa aagtcttggt
    37201 acagctgatt caagcacaag agagcggaga agtgagggaa tattatggtc tttaccattt
    37261 ggccattctc ttgcccacac gcaaggcttt agctgatgtc ttgaaacacc tgacagattt
    37321 acaaattcct cttgtcggcg gtgcagacca tggttacagt gaagctattt acttggaaga
    37381 tttggaggga aatggcattg aactctatcg agataagcca gtttccacat gggatattcg
    37441 agaagatgga cgcattatcg gagtgacaga ggctcttgcg gcgcaggaca tttatgagtt
    37501 gggggaaaga gtagagccct ttatcctggc agaaggtacg agaatggggc atattcatct
    37561 ttccgtcaag gatagtcgaa agtccagcca gttttatcaa aaggtgttag ggctagagga
    37621 taaattcagt gtgcctagcg ctagttggat tgcggctgga gattaccatc atcatttagc
    37681 agttaacgaa tggggaggaa aaggtctggc ttcgcgtaag caaggcttac caggtttagc
    37741 ctactatgtc atcgaagtcg cacacaaaaa agaactatta acgattgccc aacgagcaca
    37801 agaagttgac gcaccaatca aatggatgac atcgagccaa ttggaaatca cagactcaga
    37861 tggcatcgtg acccgtattc gtttagcaag gtaaaattag gaatttttaa taagttataa
    37921 gtattgctag tttgcttttt gaacggtctt tggtaacaga actgcgtgag ctggatgggc
    37981 gagaattttg acaaagaagc tgattagaga tataatgaag aaaaatcgag caaggaaaaa
    38041 gaaatgacaa attcaaagta tattacttgt ttgaaacgtt cagagggcca gttgtgtggg
    38101 attcaaaaga tgattgaagg agatcgtgac tgtgctgata ttgtgacgca actgacagca
    38161 gtgagatcta gtgtagagcg cgtgattgag atgataatta ccgaaaatct tactgaatgt
    38221 atcaatcagc cactagatga ttctgaagct caaaaggaac gcttagaaaa ggctatccga
    38281 tacttgatta aacggaaata aatgaatgtt ttgagacgat atgtgcaaag gtctattaac
    38341 tattagaata gtttaatggg agtcaaaaga agttgtaaaa aatctttaaa attaaaaaaa
    38401 cgcatagtat caggtattga aatgtactga ccccaaaaag ttagattttt tctgtctaac
    38461 ttttggggtg cagttcataa gaaccttggt aatatgcgtt ttattatggg gagatttata
    38521 cttttcgaaa atctcttcaa accaggtcag cttcaccttg ccgtagatat ggttactgac
    38581 ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt cagttccatc
    38641 cacaacctta aaacagtgtt ttgagctgac ttcgtcagtt ccatccacaa ccttaaaaca
    38701 gtgttttgag cagcctgcgg ctaacttcct agtttgcact ttaattttca ttgagtatta
    38761 cttcatcttc tcatgaaatt gagtttttgc ccagttcctc ttgattattc tacttgacca
    38821 ggccaagcat tcataccacc ttctacgttg gtaacggtga ggccttggga gatgagaaat
    38881 tggcaggtag atgcagagcg gactcctcct tgacaaataa catagtattc atggtcaggt
    38941 ttgagttctt tgtagccttg ctctaaggta cttaatggaa gatttttggc acctggtgca
    39001 tgtcctgctt ggaattcatg tgcttcacgg acatcgataa gttctagatt ttcattttgg
    39061 tattttccat aaaagtcagc catgctgata ctagtttcca tattctactc cttctgggtt
    39121 aaccattttg tatagtgaat aagcgccgtc aaggttttgg gcggtaaatc ctgcttgctt
    39181 gaggataggc tctgcgatat agctgcgcaa accactgtgg cagctgacga tgtaggcttg
    39241 ttttttgtcc agttcatcag cttcctcctt gacactttgt cagtttttga tacaatagta
    39301 caaaattaga ggaggtgggc tatgattcag aaacatgcga ttcctatttt agagtttgat
    39361 gacaatcctc aggcggttat catgcccaat cacgaggggc tggacttgca gttgccaaag
    39421 aagtgtgttt atgcattttt agatgaggag attgaccgct atgcaaggga agtaggggcg
    39481 gattgtgtcg gcgaattcgt ttctgctacc aagacctatc cagtctatgt catcaactac
    39541 aagggtgagg aggtctgtct ggctcaggct cctgttggct ccgctccagc agcccagttt
    39601 atggattggt tgattggcta tggtgtggag cagattatct ctactgggac ctgtggtgtt
    39661 ctagctgata tagaggaaaa tgcctttcta gtccctgttc gcgctctgcg agatgaagga
    39721 gccagttacc actatgtggc accttctcgt tatatggaaa tgcagccaga ggctattgct
    39781 gctattgagg aagttttgga agacagaggg attccttatg aagaagtcat gacctggacg
    39841 acagacggtt tttaccgaga aacggctgaa aaggtggctt atcgtaagga agaaggctgt
    39901 gctgttgtgg agatggagtg ttctgctctt gcggcagtag ctcaattgcg tggggttctc
    39961 tggggtgaat tgttgttcac agcagattct ctagcggact tggaccagta cgacagtcgt
    40021 gactggggct cggaagcttt taataaggcg ctagaactga gtttagcaag tgttcaccac
    40081 ctttagttgt actggcaaag gatttgtttt atcataaaat gtctagctca tacttttcaa
    40141 aaatatgttt aaacgaggtc accttcctct tgtcctaagc atgttgaggt tgggaaaaat
    40201 ctttaaaatc agaaaaacgt atcatatcag gtgatgaaaa ccttgatact atgcgtttta
    40261 tgttggtaag atttagagtg agatgaaatg atactcttcg aaaatctttt caaaccaggt
    40321 cagcttcacc ttgccgtagg tatatgttac tgacttcgtc agtcttatcc ggcaacctca
    40381 aaacggtgtt ttgagctgac ttcgtcagtt ctatttgcaa cctcaaaaca gtgttttgag
    40441 caatctgtga ctagctttct aatcgatgcc ttggttctca ttgcctataa tcaaaaagag
    40501 aaattttctc ctgaaaagca tatagagtag ctggcgttaa aagctcctgt cttgcttttt
    40561 tgacctatag tcacatctat caagtattgt tcttgcctaa gctatcaata aaaaggtggc
    40621 attttttagg cttggtgtta gtagattttg ccttatccta tctaagtcat ttcgaacttt
    40681 ttatggtaca atggaaacat gttattcaaa ttatctaagg aaaaaataga gctaggctta
    40741 tctcgtttat cgccagcccg tcgtattttt ttgagttttg ccttggtcat tttactaggc
    40801 tctcttcttt tgagcttgcc ctttgtccaa gttgaaagct cacgagcgac ttattttgat
    40861 catcttttca ctgctgtctc tgcagtctgt gtgacaggtc tctcaaccct tccagtagct
    40921 cacacctata atatatgggg ccaaataatc tgtttgctct tgattcagat cggtggtcta
    40981 gggctcatga cctttattgg ggttttctat atccagagca agcaaaagct tagtcttcgt
    41041 agccgtgcaa ctattcagga tagttttagt tatggagaaa ctcgatcttt gagaaagttt
    41101 gtctattcta tttttctcac gacctttttg gttgagagct tgggagctat tttgcttagt
    41161 tttcgcctta ttcctcaact tggctgggga cgtggtcttt ttagttccat ttttctagcg
    41221 atctcagcct tctgtaatgc cggttttgat aatttaggga gcaccagttt atttgctttt
    41281 cagaccgatt tactggtcaa tctggtgatt gcaggcttga ttattacagg cggccttggt
    41341 tttatggtct ggtttgattt ggctggtcat gtaggaagaa agaaaaaagg acgtctgcac
    41401 tttcatacga agcttgtact attgttgact ataggtttgt tgttatttgg aacggcaact
    41461 actctctttc ttgagtggaa caatgctgga acgattggca atctccctgt tgccgataag
    41521 gttttagtta gcttttttca aacagtgacg atgcgaacag ctggcttttc tacgatagat
    41581 tatactcagg ctcatcctgt gactcttttg atttatatct tacagatgtt tctaggtggg
    41641 gcacctggag gaacagctgg gggactcaag attacgacat tttttgtcct cttggtcttt
    41701 gcacgaagtg agcttctagg cttgcctcat gccaatgttg cgagacgaac gattgcgccg
    41761 cgaacggttc aaaaatcctt tagtgtcttt attatctttt tgatgagctt cttgatagga
    41821 ttgattctgc tagggataac agccaaaggc aatcctccct ttatccacct catatttgaa
    41881 accatttcag ctcttagtac agttggtgta acggcaaatc tgactcctga ccttgggaaa
    41941 ttggctctca gtgttatcat gccacttatg tttatgggac gaattggtcc cttgaccttg
    42001 tttgttagct tggcagatta ccatccagaa aagaaagata tgattcacta tatgaaagca
    42061 gatattagta ttggttaaga aaggaaagag catgtcagat cgtacgattg gaattttggg
    42121 cttgggaatt tttgggagca gtgtcctagc tgccctagcc aagcaggata tgaatattat
    42181 cgctattgat gaccacgcag agcgcatcaa tcagtttgag ccagttttgg cgcgtggagt
    42241 gattggtgac atcacagatg aagaattatt gagatcagca gggattgata cctgcgatac
    42301 cgttgtagtc gcgacaggtg aaaatctgga gtcgagtgtg cttgcggtta tgcactgtaa
    42361 gagtttgggg gtaccgactg ttattgctaa ggtcaaaagt cagaccgcta agaaagtgct
    42421 agaaaagatt ggagctgact cggttatctc gccagagtat gaaatggggc agtctctagc
    42481 acagaccatt cttttccata atagtgttga tgtctttcag ttggataaaa atgtgtctat
    42541 cgtggagatg aaaattcctc agtcttgggc aggtcaaagt ctgagtaaat tagacctccg
    42601 tggcaaatac aatctgaata ttttgggttt ccgagagcag gaaaattccc cattggatgt
    42661 tgaatttgga ccagatgacc tcttgaaagc agatacctat attttggcag tcatcaacaa
    42721 ccagtatttg gataccctag tagcattgaa ttcgtaaaga gggatgaccc ctcttttttg
    42781 atgcccaaga tggcaaatag agacagaagc cccttgtctt ctagtaaaag ttcttcaaag
    42841 gctggacttt atggtaaaat agaaggaagt gacaagagag agtaatactc aatgaaaatc
    42901 aaagatcaaa ctaggaaact agctgcgggc tgctcaaaac actgttttga ggttgtggat
    42961 agaactgacg aagtcagctc aaaacactgt tttgaggttg cagatagaac tgacgaagtc
    43021 agtaacatct atacggcaag gcgacgttga cgcggtttga agagattttc gaagagtata
    43081 agaaaaaatc agtcccctaa aggagcagat tatgaagtta ttgtctatcg ccatttctag
    43141 ctataatgca gcagcctatc ttcattactg tgtggagtcg ctagtgattg gtggtgagca
    43201 agttgggatt ttgattatca atgacgggtc tcaggatcag actcaggaaa tcgctgagtg
    43261 tttagctagc aagtatccta atatcgttag agccatctat caggaaaata aatgccatgg
    43321 cggtgcggtc aatcgtggct tggcagaggc ttctgggcgc tattttaaag tagttgacag
    43381 tgatgactgg gtggatcctc gtgcctactt gaaaattctt gaaaccttgc aggaacttga
    43441 gagcaagggt caagaggtgg atgcctttgt gaccaatttt gtctatgaaa aggaaggtca
    43501 gtctcgtaag aagagtatga gttacgagtc agtcttgcct gttcggcaga tttttgacta
    43561 ggaccaggtc ggaaatttct ccaaaggcca gtatatcatg atacactcgc tgatttatcg
    43621 gacagatttg ttgcgtgcta gccagttcta actgcctgaa catacttttt atgtcgatga
    43681 tctctttgtc tttacgcccc ttcagcaggt caagaccatg tactatctgc ctgtcgattt
    43741 ctatcgttat ttgattgggc gtgaggacca gtctgtcaat gagcaagtga tgattaagcg
    43801 cattgaccag caactcaagg tcaatcgact cttggtagac cagcttgatt tgtcccaagt
    43861 gagtcatcct aaaatgcgag aatatctgct gaatcatatt gaaatcacga cggtgatttc
    43921 cagtaccctg ctcaaccgat ctgaaacagc ggagcatctg gcaaaaaaac gccaattgtg
    43981 gacctatatt cagcagaaaa atccagaagt ctttcaggct attcgtaaga ccatgttgag
    44041 ccgtttgacc aaacattctg tcttgccaga tcgcaaactg tccaatgtcg tctatcaaat
    44101 caccaaatct gtttatggat ttaattaata taagtgtttt ataagaggga tttaagaaaa
    44161 attttaactt tttcttagtc ctttttaatt tcaggagatt atactagagt catcaaataa
    44221 agaaagactc taaggagaat cctatgaaat tcaatccaaa tcaaagatat actcgttggt
    44281 ctattcgccg tctcagtgtc ggtgttgcct cagttgttgt ggctagtggc ttctttgtcc
    44341 tagttggtca gccaagttct gtacgtgccg atgtggtcaa tccgacccca ggtcaagtct
    44401 tacctgaaga gacatcggga acgaaagagg gtgacttatc agaaaaacca ggagacaccg
    44461 ttctcactca agcgaaacct gagggcgtta ctggaaatac gaattcactt ccgacaccta
    44521 cagaaagaac tgaagtgagc gaggaaacaa actcttctag tctggataca ctttttgaaa
    44581 aagatgaaga agctcaaaaa aatccagagc taacagatgt cttaaaagaa actgtagata
    44641 cagctgatgt ggatgggaca caagcaagtc cagcagaaac tactcctgaa caagtaaaag
    44701 gtggagtgaa agaaaataca aaagacagca tcgatgttcc tgctgcttat cttgaaaaag
    44761 ctgaagggaa aggtcctttc actgccggtg taaaccaagt aattccttat gaactattcg
    44821 ctggtgatgg tatgttaact cgtctattac taaaagcttc ggataatgct ccttggtctg
    44881 acaatggtac tgctaaaaat cctgctttac ctcctcttga aggattaaca aaagggaaat
    44941 acttctatga agtagactta aatggcaata ctgttggtaa acaaggtcaa gctttaattg
    45001 atcaacttcg cgctaatggt actcaaactt ataaagctac tgttaaagtt tacggaaata
    45061 aagacggtaa agctgacttg actaatctag ttgctactaa aaatgtagac atcaacatca
    45121 atggattagt tgctaaagaa actgtcgaaa aagctgtta
//
LOCUS       DAVBCV010000010        42569 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBCV010000010 DAVBCV010000000
VERSION     DAVBCV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.010"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..279
                     /locus_tag="VQ516_000548"
     CDS             <1..279
                     /locus_tag="VQ516_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2511039.1"
                     /translation="WQYQHDSYHQFLEGKGIQASMSRKGNSQDNGMMESFFGILKSEM
                     FYGYEKSFRSLENLEQAIVDYIDYYNNKRIKVKLKELSPVQYRTKSFG"
     gene            complement(327..2870)
                     /locus_tag="VQ516_000549"
     CDS             complement(327..2870)
                     /locus_tag="VQ516_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2511040.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(2932..4554)
                     /locus_tag="VQ516_000550"
     CDS             complement(2932..4554)
                     /locus_tag="VQ516_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2511041.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            4760..5785
                     /gene="trpS"
                     /locus_tag="VQ516_000551"
     CDS             4760..5785
                     /gene="trpS"
                     /locus_tag="VQ516_000551"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2511042.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            5938..7416
                     /gene="guaB"
                     /locus_tag="VQ516_000552"
     CDS             5938..7416
                     /gene="guaB"
                     /locus_tag="VQ516_000552"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2511043.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(7467..8564)
                     /gene="recF"
                     /locus_tag="VQ516_000553"
     CDS             complement(7467..8564)
                     /gene="recF"
                     /locus_tag="VQ516_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2511044.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(8567..8935)
                     /gene="yaaA"
                     /locus_tag="VQ516_000554"
     CDS             complement(8567..8935)
                     /gene="yaaA"
                     /locus_tag="VQ516_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HET2511045.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKIDIPDMNIDILLTQSTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            9094..10344
                     /locus_tag="VQ516_000555"
     CDS             9094..10344
                     /locus_tag="VQ516_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2511046.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSYLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHCPSKYGDEKHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSQLDLFSGFLRMYAGIDRENRNQARKMMNNQLLDL
                     KKGYFTELELEQTKEMIRRSLLLSQDNQSSLIERAYQNALLGKSSADFKSWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            10341..11624
                     /locus_tag="VQ516_000556"
     CDS             10341..11624
                     /locus_tag="VQ516_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2511047.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTLVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELITSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKLTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            11666..12487
                     /locus_tag="VQ516_000557"
     CDS             11666..12487
                     /locus_tag="VQ516_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2511048.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            12498..13043
                     /gene="pgsA"
                     /locus_tag="VQ516_000558"
     CDS             12498..13043
                     /gene="pgsA"
                     /locus_tag="VQ516_000558"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2511049.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            13040..13867
                     /locus_tag="VQ516_000559"
     CDS             13040..13867
                     /locus_tag="VQ516_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2511050.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            13852..14691
                     /locus_tag="VQ516_000560"
     CDS             13852..14691
                     /locus_tag="VQ516_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2511051.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPNQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            14684..15478
                     /locus_tag="VQ516_000561"
     CDS             14684..15478
                     /locus_tag="VQ516_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2511052.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            15537..16355
                     /gene="mreC"
                     /locus_tag="VQ516_000562"
     CDS             15537..16355
                     /gene="mreC"
                     /locus_tag="VQ516_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2511053.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKVSQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            16355..16849
                     /gene="mreD"
                     /locus_tag="VQ516_000563"
     CDS             16355..16849
                     /gene="mreD"
                     /locus_tag="VQ516_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2511054.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            16943..18121
                     /locus_tag="VQ516_000564"
     CDS             16943..18121
                     /locus_tag="VQ516_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2511055.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            18345..19124
                     /gene="rpsB"
                     /locus_tag="VQ516_000565"
     CDS             18345..19124
                     /gene="rpsB"
                     /locus_tag="VQ516_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2511056.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            19203..20243
                     /locus_tag="VQ516_000566"
     CDS             19203..20243
                     /locus_tag="VQ516_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2511057.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            20490..20831
                     /gene="tnpB"
                     /locus_tag="VQ516_000567"
                     /pseudo
     CDS             20490..20831
                     /gene="tnpB"
                     /locus_tag="VQ516_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(20967..21254)
                     /locus_tag="VQ516_000568"
                     /pseudo
     CDS             complement(20967..21254)
                     /locus_tag="VQ516_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            21245..21643
                     /locus_tag="VQ516_000569"
     CDS             21245..21643
                     /locus_tag="VQ516_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2511058.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPIIRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(21840..22760)
                     /gene="cysK"
                     /locus_tag="VQ516_000570"
     CDS             complement(21840..22760)
                     /gene="cysK"
                     /locus_tag="VQ516_000570"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2511059.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(23045..23680)
                     /locus_tag="VQ516_000571"
     CDS             complement(23045..23680)
                     /locus_tag="VQ516_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2511060.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVSVNLV"
     gene            23736..25034
                     /locus_tag="VQ516_000572"
     CDS             23736..25034
                     /locus_tag="VQ516_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2511061.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            25031..25693
                     /locus_tag="VQ516_000573"
     CDS             25031..25693
                     /locus_tag="VQ516_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2511062.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQACQFWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            25773..26321
                     /gene="raiA"
                     /locus_tag="VQ516_000574"
     CDS             25773..26321
                     /gene="raiA"
                     /locus_tag="VQ516_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2511063.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            26457..28430
                     /locus_tag="VQ516_000575"
     CDS             26457..28430
                     /locus_tag="VQ516_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2511064.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDALGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNTVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            28427..28879
                     /gene="rplI"
                     /locus_tag="VQ516_000576"
     CDS             28427..28879
                     /gene="rplI"
                     /locus_tag="VQ516_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2511065.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            28923..30275
                     /gene="dnaB"
                     /locus_tag="VQ516_000577"
     CDS             28923..30275
                     /gene="dnaB"
                     /locus_tag="VQ516_000577"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2511066.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            30277..30543
                     /locus_tag="VQ516_000578"
     CDS             30277..30543
                     /locus_tag="VQ516_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511067.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            30632..31978
                     /gene="cbpD"
                     /locus_tag="VQ516_000579"
     CDS             30632..31978
                     /gene="cbpD"
                     /locus_tag="VQ516_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2511068.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      32061..32163
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            32234..32524
                     /locus_tag="VQ516_000580"
     CDS             32234..32524
                     /locus_tag="VQ516_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2511069.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            32487..33245
                     /locus_tag="VQ516_000581"
     CDS             32487..33245
                     /locus_tag="VQ516_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2511070.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            33283..34290
                     /locus_tag="VQ516_000582"
     CDS             33283..34290
                     /locus_tag="VQ516_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2511071.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            34290..35018
                     /locus_tag="VQ516_000583"
     CDS             34290..35018
                     /locus_tag="VQ516_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511072.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            35137..35595
                     /locus_tag="VQ516_000584"
     CDS             35137..35595
                     /locus_tag="VQ516_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2511073.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            35597..38029
                     /locus_tag="VQ516_000585"
     CDS             35597..38029
                     /locus_tag="VQ516_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2511074.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFMELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALVLGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     ALRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            38671..39324
                     /gene="rr06"
                     /locus_tag="VQ516_000586"
     CDS             38671..39324
                     /gene="rr06"
                     /locus_tag="VQ516_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2511075.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            39321..40661
                     /gene="hk06"
                     /locus_tag="VQ516_000587"
     CDS             39321..40661
                     /gene="hk06"
                     /locus_tag="VQ516_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2511076.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILFLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            40744..41271
                     /locus_tag="VQ516_000588"
     CDS             40744..41271
                     /locus_tag="VQ516_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2511077.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            41350..>42569
                     /locus_tag="VQ516_000589"
     CDS             41350..>42569
                     /locus_tag="VQ516_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET2511078.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGDEVESHLKKILSEIQTQLDRKRHAKTVALINKLQDIKRTYSYKLNVLEEK
                     PKAELTSKTKEELTAAFEQFKKDTLPTEPGKKVAEAEKKVAEAEKKAEDQKEEDRRNY
                     PTNTYKTLELEIAEFDVKVKEAELELLKEKAKKPLNEGTIKQAKEKVESKKAEATRLE
                     NIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPAPKPENP
                     APKPEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQ
                     PSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYL
                     NANGSMATGWLQNNG"
BASE COUNT        13450 a         6962 c         9181 g        12976 t
ORIGIN      
        1 tggcaatatc aacacgattc ttatcatcag tttcttgagg gtaagggaat tcaagcatct
       61 atgtcacgca agggaaacag ccaagacaac ggtatgatgg aatctttctt tgggattctg
      121 aaatcggaaa tgttttacgg ttatgagaag agctttagat ctttagaaaa ccttgaacaa
      181 gctattgtgg actacattga ttactacaac aacaaacgaa ttaaggtaaa actaaaagaa
      241 cttagccctg tgcaatacag aactaaatcc ttcggataaa ttaattgtct aactttttgg
      301 ggtcagtaca ttaactggtc gattttttat gccttagatg actttcgtct gtgattatag
      361 attccaaata gtaagagaga agtaaaggaa cagattgctc cagtaataaa accattggga
      421 atgaaggaaa gtgtaatagt tcctttcccc ttgggaatgt caactttcat aaatccagtt
      481 tgagcttgtt taatttctat tttcttacca tcttggtagg cagaccaacc tttgtcataa
      541 ggaatggtga agaaaataga tgtatcttgt tggacatcat atgtagcaaa aaccttgttt
      601 ttagaagttg atactgtgac aggttgttct ttaatttttt gaattgcctc ggtgaaagtt
      661 ttggtatcta aacgatagaa ggtaggagat tcaaatgata cttgtgaatt tccagggaaa
      721 ctaacattga tattgaaagt ttttttctct ttagtatatc ctagattaaa gaaggagaag
      781 acattatcag ttgtaaaagt ctttttttca ccatttacaa ggatgtcaac cttcttttgt
      841 ttatcgttag aaaagtgaag gtttatgaaa gagagataaa cttggctgtt ttctggaact
      901 tcaatttgat actggattgc tgcatcttca tttgaagaac ttgtgacact aatcaaatca
      961 ttagtatttt ctgttttttc ataaggtatt ggagaaaaat aatcaaaatt gacgttagca
     1021 agttgattta aaaatgaggc ctgattatcc aaggtatgtt cattgaactt gacatcattg
     1081 taaacagatt gactagcaac tgcaatcgga agagagtatt gattttcata tagggtaaga
     1141 ttatcttttt gatagatatc tttaaagcca tacttatcaa taggactgtc tgagatattg
     1201 tactggatac caaataaact atcagccaaa atactattat ttgcatatcg gagattgaga
     1261 ttagtcccag aggatttaaa accaagttta tctaaagtag agcttgatga acgatttcga
     1321 acagatgaaa attgagagat tccattgtag ttgaatttca tactgtcatt tcctgtctga
     1381 gtttgtagtt tttcagtacg agtaaattga tttccaatat atgttgagaa agattccata
     1441 gctgggatat ctcgactata agcacttcga gaagcaaatc cccattcctt agcaattccg
     1501 tccatttgag atgaagcatt taaactcatt tcaaccagta taaataaaga gattagaatg
     1561 gcaaatagat tcacagatat aaactttttg ataactgcaa ggagtaaaag agaatagaca
     1621 accaaaaatt caagagtaag cagaatattc aaatctgtta aaaaagaata atgcgatttt
     1681 agatagatgg tagctaaaaa tcctgctact acaagaaaaa gcgaaactaa aaaattccag
     1741 actttaagtt ctttcagacg ctttaagact tctgctgctg tgtaaattaa caaggtagag
     1801 aaaatccaag catagcgatg taaaaacatg tttggagtat gcatgccttg ccaaaataag
     1861 tcaagagctt ctatgtaaaa gcttgcaatt agaaatgcaa agaatattac atatatgagt
     1921 ttcacgtgaa acttaataga tttcagcgta aaaaataaaa tggtcaaaat aaagggaaat
     1981 agtccaacaa aaatcattgg gatggcccca tactttgttg tgtcaaagga accaatgaat
     2041 tgcttagcaa agagatcaag ataccagcta ctttcagttt gaaactttgt aacttcagtc
     2101 aatttttccc catgtgtctg taaatcaaat agagtgggaa gagtcataat caaactagcc
     2161 ataccagcta aaaaggagat aactatgaaa tcaagaacag atgattttcg agtcttaaag
     2221 tcccacgaaa tttgacagag ataccagaaa ataagaaaca atactgtcat atatccaaaa
     2281 taataatttt gaataaataa gattgacaga cttgtaaagt acaataggag tttcttttca
     2341 gttatcagta gatgtaaacc agttataatt aaaggaatca agataaaaac atctagccag
     2401 gtttttatct ctaattgact gacagtgaaa ctcatcagag cataggaagt agataaggct
     2461 agttttaaag tctgagggat agattgaaac aatttattca aactaaaaaa ggttgacaga
     2521 ccaatcaatc caaattttaa gagagtggtc agatagacag catctggcat attcgttaga
     2581 tcaaaaaagt aaactagagg cgcgagaaaa ctacccaagt aataactaga tagggcatag
     2641 aagtttagcc ctagaccact tgtaaaggtg taaaacagac tactatttcc atgtaggata
     2701 tttcgtaagg ctacatcaaa aataacgtat tgatgaaagc catctcctaa tagaggagag
     2761 ttgtcgctat tccagtagat accttgagat agatatactc cagacataat cactacagga
     2821 atgatgaaag aaataaaata ggttcgatat gtttttaaaa atgatttcat gttacctcgt
     2881 agaatgatag aaaactcagt tggttaaccc aactgagttt tgaagtttta tttagtcttt
     2941 ccaaagttct ttaacttttg cttgtacttc tgcattttct aggaattcat cgtaggtttc
     3001 atcgatacgg tcaatgacgc catttttaga taagacaatg atatggttag ccaaagtttg
     3061 aataaactcg tggtcatggc tggcaaagat gattgattct ttaaagtttt tcagtccatc
     3121 attcaagctt gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa gtacaaggac
     3181 atttgatttt aagagcatga gttttgaaag catgacacga actttttctc cccctgacaa
     3241 gacatttaca ggtttgttaa cttcatctcc agagaagagc atacggccga ggaaaccacg
     3301 taggaaagta ttgtcatctt cttctttact tgcgaattga cgcaaccagt caagaattga
     3361 ttctcctcct gcaaaatcag ctgagttatc ttttggtagg taagattgac tagttgtaac
     3421 tccccacttg acagttcctt catagtcaat atctcccatg attgcacgaa ttaatgcagt
     3481 cgtttgaata tcattttgtc caataagtgc tgtcttatca tctggacgca agatgaaact
     3541 aatattatcc aagatagttt caccatcaat ctttacagtt agattttcta ctgtcaagag
     3601 atcattacca atctcacgtt ccgctttaaa gttgataaat ggatatttac gactagatgg
     3661 tacaatctct tctagctcaa tcttatcaag cattttctta cgtgatgttg cctgccttga
     3721 cttagaagca ttggcagaga aacgagcaac gaattcttgc aattgtttaa ttttttcttc
     3781 tgctttagca ttacggtctg ctagcaattt agcagcaagc tcagaagatt ccttccagaa
     3841 gtcgtagttt ccgacataga gtttgatttt tccaaagtca aggtcggcca tgtgagtaca
     3901 aactttgttt aagaagtgac ggtcgtggga tactacgata actgtgttat caaagtcaat
     3961 caagaagtct tctaaccaag taatcgattg gatatccaaa ccgttagtag gctcgtccaa
     4021 gagaagaaca tctggtttac caaaaagtgc tttggcgagg agaaccttta ctttttcacc
     4081 gttggccaat tcgctcatgt tttggtagtg taattcttct ggaatgttta ggttttgaag
     4141 tagttgagag gcttcactct ctgcttccca acctccaagt tcggcaaact ctccttcgag
     4201 ttcggcagca cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga tagcatcttt
     4261 ctctttcatg atgctataaa gtttttcatt tcccatgata acgacatcaa tggcacgttc
     4321 atcttcgtag tcaaagtgat tttgacgaag aacagagaga cgttcatctg gaccaagaga
     4381 gatgtgacca gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg ttgattttcc
     4441 ggcaccatta gcaccgatta atccgtaagt atttccttct gtaaatttga tattgacatc
     4501 atcaaaaagt ttgcgatcac taaaacgtag tgaaacatca gatactgtaa gcaatgtttt
     4561 tctcctatat gtgtaatata tttattctac tagaaaatac agaaatattc aaatttttat
     4621 ttgtcaattt tgtgtaaatt atatttacag tatcctttac acaaatctgt aaaaagcaag
     4681 gctgatttat tttgataaat tacggttatt tcattaaaaa aatgctataa ttgaaaggac
     4741 tatatcgaag gagaacaaaa tgactaaacc cattatttta acaggagacc gtccaacagg
     4801 aaaattgcat attggacatt atgttggaag tctcaaaaat cgagtattat tacaggaaga
     4861 ggataagtat gatatgtttg tgttcttggc tgaccaacaa gccttgacag atcatgccaa
     4921 agatcctcaa accattgtag agtctatcgg aaatgtggct ttggattatc ttgcagttgg
     4981 attggatcca aataagtcaa ctatttttat tcaaagccag attccagagt tggctgagtt
     5041 gtctatgtat tatatgaatc tagtttcgtt agcacgtttg gagcgaaatc caacagtcaa
     5101 gacagagatt tctcagaaag gatttggaga aagcattccg acaggattct tggtctatcc
     5161 aatcgctcaa gcagctgata tcacagcttt caaggctaat tatgttcctg ttgggacaga
     5221 tcagaaacca atgattgagc aaactcgtga aattgttcgt tcttttaaca atgcatataa
     5281 ctgtgatgtc ttggtagagc cggaaggtat ttatccagaa aatgagagag cagggcgttt
     5341 gcctggttta gatggaaatg ctaaaatgtc taaatcacta aataatggta tttatttagc
     5401 tgatgatgcg gatactttgc gtaaaaaagt aatgagtatg tatacagatc cagatcatat
     5461 ccgcgttgag gatccaggta agattgaggg aaatatggtt ttccattatc tagatgtttt
     5521 tggtcgtcca gaagatgctc aagaaattgc tgatatgaaa gaacgttatc aacgaggtgg
     5581 tcttggtgat gtgaagacca agcgttatct acttgaaata ttagaacgtg aactgggtcc
     5641 tattcgtgag cgccgtattg aatttgctaa ggatatggga gaagtttata atatgattca
     5701 aaaaggtagt gaaagagcgc gtgaagttgc gggtcaaacc ctatctgagg taaaaggggc
     5761 aatgggactt cattacttta actaagttta ttttacaaga aactatcatt tctatctcga
     5821 agaaaagata gtttttttat ttacccctgt aacatttgac aaatttttat agaatggtat
     5881 gatagataca atataaaaag agtcaagctc aaaaagaaag aaaagaggaa acttcgaatg
     5941 tctaattggg acactaaatt tttgaaaaaa ggttttacct ttgatgatgt attgcttatt
     6001 ccagctgaaa gtcatgtgtt gcctaacgat gcagatttaa caactaaatt ggcagataat
     6061 ttgactttaa atatcccaat tattaccgct gccatggaca cagttacaga gagtcaaatg
     6121 gccattgcta ttgctcgtgc aggcggtctc ggagttatcc ataaaaacat gtcaattgct
     6181 caacaagcag acgaggttcg taaggtaaaa cgttctgaaa atggagttat tattgatccg
     6241 ttcttcttga cgcctgaaca tacaattgct gaagcagatg agcttatggg tcgttaccgc
     6301 atcagtggtg ttccagttgt tgaaacactt gaaaatcgta aattggttgg tattttgaca
     6361 aaccgagatc ttcgttttat ttcagattat aatcaaccaa tttcaaacca tatgactagt
     6421 gaaaatcttg ttactgctcc tgtgggtacg gatcttgcaa cggctgagag tattcttcaa
     6481 gagcatcgta ttgaaaaact tccgttggtc gatgaagaag gcagtctttc tggtttgatc
     6541 actatcaaag atattgaaaa agttattgag tttccaaatg cggctaaaga tgagtttggt
     6601 cgtcttctag ttgcaggtgc agtaggtgtt acttcagata catttgaacg tgcagaggct
     6661 ctttttgagg caggagcgga tgcgattgtt attgatactg cacatggtca ttctgcaggt
     6721 gtcttgcgta aaattgccga gattcgtgct catttcccag atcggacttt gattgctgga
     6781 aatattgcta ctgctgaagg tgcacgtgcc ctttatgaag cgggtgtaga cgttgttaag
     6841 gttggtattg gaccaggttc tatctgtact actcgtgtga ttgctggtgt tggtgttccg
     6901 caagtaacag ctatctacga tgctgcagct gttgcgcgcg aatatggtaa aacgattatt
     6961 gctgacggtg ggatcaagta ttctggagat attgtaaaag cacttgctgc aggtggaaat
     7021 gctgttatgc ttggatctat gtttgctgga actgatgaag ctccaggcga aactgaaatc
     7081 ttccaaggac gtaaattcaa gacttaccgt ggtatgggat caattgctgc tatgaagaaa
     7141 ggttcaagcg accgttattt ccaaggttct gtcaatgaag caaacaagct tgttccagaa
     7201 ggaattgaag gtcgtgttgc ttataaagga gcggcagctg atattgtttt ccaaatgatt
     7261 ggtggtattc gctctggtat gggttactgt ggtgcagcta accttaaaga actacacgat
     7321 aatgctcaat ttattgaaat gtctggtgct ggtttgaaag aaagccatcc tcatgatgtg
     7381 caaattacta atgaggcacc aaattattct atgtaaaaaa caatgaaaag aactccagtg
     7441 aaaacaggag ttcttttaca atgttgtcaa tttccattta cagcagcttt accatcctga
     7501 atagtgaaga tacttagatt ttctggcaga ttttgaagat ggtctaagct tgttgttgtg
     7561 ataaaggttt ggattgattg agaaatcgtt tctaataatt ttaactgtct agtgttgtca
     7621 agttcactca tcacatcgtc aagcagtaat ataggagatt ctgtggtaat gctttccatt
     7681 aattcgattt ctgctaattt tatcgagagg acgagactac gatgttgacc ttggcttccg
     7741 aaactagcat ccatcccatt tatataaaaa gaaatgtcat ctcgatgagg accgacacca
     7801 gtattctttt taaataaatc tctggatcta cttttttcta aagcaatttt gaaagattcg
     7861 gataagtttt gtttgtcagt tatattgaca gaagattgat aggatattga caactcttcg
     7921 atctgattag agagttcaaa atgtttctta cgcccaaatg attctagttt ttttatgaaa
     7981 tctaagcggt gattcattac acgacatcca taatcaacta gctgatcatc taacacagaa
     8041 aggaatgttt catctatttt ttgagctgat tttaggtaag tgtttctttg ctttaggatg
     8101 tggttataat tggttaagtc agataaatag attggcttaa tttgcccaag ttccatatca
     8161 atgaattttc gtcgaatcga aggtgctcct ttaattagtt gtaaatcttc aggagcaaat
     8221 aagacaacat tcatgtgtcc tacataatct gaaaggcgtg cctgttttaa gtgattaact
     8281 tttgttacac ggcctttttg tgttagttcg atttctaggg gaatggatcc agtttttttc
     8341 tgaacgagac ctgaaagatg aagttgttcc tcatcaaaat gaatgagatt tttatctgtt
     8401 cgagttcgat gactacgcgt taaggctaaa aaatagatag cctctaacat gtttgttttt
     8461 ccttgtgcat tacgtcccaa aaagacattc aatttaggat taaagtctat tttcgtttct
     8521 ttgtaattac gaaaagtctt gagagataga tgttgtagcc acatgattat ctaccaggga
     8581 atcgtggagc ttgttggctt ttaggtgatg aagcaggttt cgatttgtct tttttaactc
     8641 ccttattcat ctttttgaca agtttagcga tccgttcttt ttcaacttta tcagcttggt
     8701 attcatcttg ttcttcagaa gtagattgtg tcaacaagat gtcaatgttc atgtcaggga
     8761 tgtcaatttt atcgccaata cgaagttttt taccacgacg actctctaat tccctattaa
     8821 agtaaacaga atgttcagag agaaatgatt taatagctcc tccgctatgt gtaattccaa
     8881 gttctttgag tagtgcttgg agggtaataa attcttcaaa taatttgtat tccataattc
     8941 acctcaatct ttggtattat accatatttt cctaaaaata gtgagagata cagcgagaaa
     9001 tgtaagcgat ttttatttca aaagtgttac agggaacaag aaaatttcag gttttcgtga
     9061 tataatagaa gtctgtatat aaggagataa atcatggagt tagtgcatgg aatttcaaca
     9121 cattttatcc aatcaaaaaa gtttaaaaca aacaaaatta ccgtgcgttt taccgctcca
     9181 ttatcccttg atacgattgc aggtcacatg ttgagtgcaa gtatgctaga gactgctaat
     9241 cagatgtacc ccacttctca agatttgagg agacacttgg ccagtctata cggtacagat
     9301 atgtcaacca attgtttcag aagagggcaa agccacatta tagaattgac atttacctat
     9361 gttcgtgatg agtttttaag taggaaaaac gtgctaactt ctcagatttt ggaacttgta
     9421 aaagaaactc ttttttcacc cgtagtagtt gataatgggt ttgatccggc cttatttgaa
     9481 attgagaaaa aacaattgct agcaagttta gcagctgata tggatgattc tttttatttt
     9541 gcacataaag aattggataa attgtttttt catgatgaac gtcttcaatt ggaatatagt
     9601 tatttacgaa atcgtatttt agctgaaact ccacaaagtt cttattcttg tttccaagaa
     9661 tttttagcca atgatcgaat agatttcttt ttcctaggtg attttaatga ggttgaaatt
     9721 caaaatgtat tagaatcatt tggctttaaa ggtcgaaaag gagatgtgaa ggttcagtat
     9781 tgtcaacctt attctaatat ccttcaggaa ggtatggttc ggaaaaatgt gggacaatcc
     9841 attttggaat taggttatca ttgcccttct aaatatggtg atgagaaaca tttacccatg
     9901 attgtaatga atggtttact tggtggattt gctcactcta aactttttac aaatgtccgt
     9961 gaaaatgctg gattagctta tactatttca agtcagctcg atttatttag tggattcttg
    10021 aggatgtatg ctggtattga ccgagaaaat cgtaaccagg ctcgtaaaat gatgaataat
    10081 caactgcttg atttaaaaaa aggttatttt acagagcttg agttagagca gaccaaggaa
    10141 atgattcgtc ggtctttgtt actttctcaa gataaccaat cttcattgat tgaacgtgct
    10201 tatcaaaatg ccttacttgg aaaatcttca gcagatttta aaagttggat tgcaaaactc
    10261 gagcaagttg acaaagatgc tatttgtaga gcagctaaca atgtgaaact acaagcgatt
    10321 tactttatgg aaggaataga atgacaaagg ttgtttttga agaaaaatac tatccagctg
    10381 taaaagaaat ggtttatcga actcgtttgg caaatggatt gacagtagct cttttgccta
    10441 aaaaggaatt taaagaggtt tacgggagtg taactgtaca gtttggttcg gtagatacgc
    10501 ttgtcacaga agttgatgga gatgtaaaac aatatcctgc aggaattgct cattttcttg
    10561 aacataaatt atttgaaaga gaagattcta gcgatttgat gtcggctttt acgagtctag
    10621 gtgcagatag taatgccttt acaagcttta caaaaacaaa ctatcttttt tcagcaacgg
    10681 attatttttt agaaaattta gatttacttg atgaattgat aacatcagca cactttactg
    10741 aagcttccat tctgacagag caggatatta ttcagcaaga acgagaaatg taccaagatg
    10801 atccagattc gtgtttattc ttttcaactt tagcgaattt gtatcctggt acacctttag
    10861 caactgatat agttggaagt gaggagtcca tttcccaaat caatctaact aatttgcaag
    10921 aaaattttac aaagttttac aaacctgtaa acatgtctct gtttttagtt ggtaattttg
    10981 atgtggagcg agtacaggac tattttgaaa gcaaagaact gaaagattca gattttcagg
    11041 aagtagcaag agaaaagttg tttttacagc ctgtaaagct aacagatagt atgagaatgg
    11101 aagtatcttc tcccaaacta gcgattggag ttagaggtaa gcgagaagtt tctgaagcgg
    11161 attgctatcg acatcatatt ttattaaaat tattgtttgc aatgatgttt ggttggactt
    11221 cggatcgttt tcaaaaatgt tatgaatcag gtaaaattga tgcgtcctta tctctggaag
    11281 ttgaaataac aagtcgcttt cattttgtca tgttgacaat ggatacgaaa gagccagttg
    11341 ctttgtctca tcaatttagg aaggctattc gtaattttac aaaggattta gatattacag
    11401 aggaacattt agatattatc aaaagagaga tgtttggcga atttttcagt agcatgaact
    11461 ctcttgaatt tattgcaacg caatatgatg cttttgaaaa tggtgagaca atttttgatt
    11521 tgccgaaaat tttacaggaa attactttag aggatgtcct tgatgctgga catcatttaa
    11581 tagatgatgg tgacatagtt gattttacaa tatttccatc gtagtaacct atcataatag
    11641 acactagaaa gaagggatga caagtatgag aaaaaaaaca attggagagg ttttacgatt
    11701 agctagaatc aatcagggat tgagtttaga tgaattgcag aaaaagacag aaatccagtt
    11761 agatatgttg gaagcaatgg aagcagacga tttcgatcaa cttccaagtc ctttttacac
    11821 gcgttctttc ttgaaaaaat atgcatgggc tgttgagtta gatgaccaaa ttgttttgga
    11881 tgcttatgat tctgggagta tgattactta tgaggaagta gatgttgatg aagatgagtt
    11941 gacaggtcgt agacgttcaa gtaagaaaaa gaagaaaaaa acatcatttt tacctttatt
    12001 ttattttatc ctttttgctt tatcgatttt aatttttgtg acttattatg tttggaacta
    12061 tattcaaact caaccagagg agccttctct ttctaattac agtgtggttc aatcaacaag
    12121 ttcaactagc tctgttcccc actcctcaag tagtagttct tctagtatag aatcagctat
    12181 aagtgtatca ggcgaaggaa atcatgtaga aatcgcttat aagacaagta aggaaacagt
    12241 taaattgcaa ttggcagttt cagatgttac aagttgggtc agtgtttcag aaagcgaact
    12301 tgagggcggt gtaaccttat cgccaaagaa gaaaagtgca gaagcaacag ttgcaactaa
    12361 aagtcctgta acaattacgt taggtgttgt aaaaggtgtt gatttgacag tagataatca
    12421 gactgttgat ttatcgaaat taacagctca gactggacaa atcactgtaa cctttactaa
    12481 aaattaagga aaaacgaatg aaaaaagaac aaattcccaa tctcttaaca ataggtcgaa
    12541 ttctctttat acctattttt atctttattt taacgatagg aaattcgata gagagtcata
    12601 tagttgcagc tattatcttt gctgttgcca gtattaccga ctatttagat ggatatttag
    12661 ctcgtaaatg gaatgtggtc agtaattttg gtaaatttgc agatcctatg gcggataagt
    12721 tactagttat gtcggctttt attatgttga ttgagttagg tatggctccg gcttggattg
    12781 ttgcagtgat tatatgtcgt gagttagctg tgacaggttt aaggctttta ttggttgaaa
    12841 ctggtggaac aattttagca gcagcaatgc ctggaaaaat taaaactttt agtcagatgt
    12901 ttgctattat tttcttgcta ttacattgga ctttgcttgg tcaagttcta ctttatgtag
    12961 ccttattttt cactatctac tctggctatg actattttaa gggtagtgcc tatgtattta
    13021 aagggacatt tggttcgaaa tgaaatcaat aattgatgta aaaaatcttt cttttcgcta
    13081 taaagaaaat cagaactact acgatgtgaa ggatattacg tttcacgtga aacgtggaga
    13141 atggctttcg attgtagggc ataatggtag tggtaaatca acgacggttc gattaattga
    13201 tggcttactg gaagcagaat ccggagagat tgtaattgat ggccaacgtt tgactgagga
    13261 aaatgtttgg aatatacgtc gtcaaatcgg tatggttttt caaaatccag acaatcaatt
    13321 tgttggagcg actgttgaag atgatgttgc ttttggtttg gaaaatcagg gactttctcg
    13381 tcaagaaatg aaaaagagag tggaagaagc tctggcttta gttggcatgt tggactttaa
    13441 aaagagagag ccagcgcgtc tatcaggtgg tcaaaagcaa cgtgtggcca ttgcaggtgt
    13501 tgtagcccta agaccagcta ttttaatctt agatgaagca acgagtatgt tggatcctga
    13561 ggggcgtaga gaacttattg ggacagtaaa aggaattcga aaagactatg atatgacggt
    13621 catttctatt acccatgatt tggaagaagt cgccatgagt gatcgcgtat tggtcatgaa
    13681 aaaaggggaa attgaatcaa ctagtagtcc aagggagctt ttctctcgaa atgatttaga
    13741 tcaaattgga ttagacgatc cttttgctaa tcaattaaaa aaatctttga gccagaatgg
    13801 ctatgattta cctgaaaatt atttgacaga aagtgagcta gaggataagc tatgggaatt
    13861 gctctagaaa atgtgaattt tacatatcaa gaaggtactc ccttagcttc agcagctttg
    13921 tcggatgttt ctttgacgat tgaagatggc tcttatacag ctttaattgg gcacacaggt
    13981 agtggtaaat caactatttt acaactctta aatggtttat tggtgccaaa tcaagggagt
    14041 gtgagggttt ttgatacctt aatcacctcg acttctaaaa ataaagatat tcgtcaaatt
    14101 agaaaacagg ttggcttggt atttcagttt gctgaaaatc agatttttga agaaacggtt
    14161 ttgaaggacg ttgcttttgg accgcaaaat tttggagttt ctgaagaaga tgctgtgaag
    14221 actgcgcgtg agaaactggc tctggttgga attgatgaat cactttttga tcgtagtccg
    14281 tttgagctgt cagggggaca aatgagacgt gttgccattg caggcatact tgccatggag
    14341 ccagctatat tagtcttaga tgagccaaca gctggtctag atcctctagg gagaaaagag
    14401 ttgatgaccc tgttcaaaaa actccaccag tcagggatga ccatcgtctt ggtaacgcat
    14461 ttgatggatg atgttgctga atatgcgaat caagtctatg taatggaaaa gggacgttta
    14521 gtaaaggggg gcaaaccaag tgatgtcttt caagacgttg tttttatgga agaagttcag
    14581 ttgggagtac ctaaaattac ggccttttgt aaacgattgg ctgatagagg cgtgtcattt
    14641 aaacgattac cgattaagat agaggagttc aaggagtcgc taaatggata gtatgatttt
    14701 agggcgttat atcccagggg attcgattgt tcaccgattg gatccacgta gcaaattgct
    14761 tgctatgatg ctactgattt tgattgtgtt ttgggctaat aaccccttga cgaatctgat
    14821 tctttttata gcgacaggga tatttattgc cttgtcagga gtatctcttt cattttttat
    14881 tcagggcttg aagtctatgt ttttcttgat tgccttcaca actatttttc aactattttt
    14941 catttctaat gggaatgttt tatttgagtt ttcgtttgtg agaatcacgg attatgcttt
    15001 gcaacaagct gggattattt tttgtcgctt tgtattgatt attttctttt caactttgtt
    15061 aaccttaaca accatgcctt taagtttggc atcagctgtc gaagctttat tagcaccttt
    15121 aaagcgtgtg aaagttccag ttcatgaaat tggattgatg ctgtctatga gtttgcgttt
    15181 tgtcccaacc ttgatggatg atacgactcg gattatgaat gcacagaaag ctcgtggagt
    15241 ggattttgga gaaggaagca tcgttcaaaa agtaaaggcg atgattccca ttttgattcc
    15301 tctttttgcg acaagtttaa aacgtgcaga ttccttggct atcgctatgg aagcgcgtgg
    15361 ctatcagggt ggaaaaggca gaagtcaata ccgacaattg aaatggactc taaaggatac
    15421 gctgaccatt cttgttattc tcgtacttgg ttgttgttta tttttcttaa aatcttagta
    15481 gctttcagga attgataaaa agttactgta acagtttttg atataaaggt aggaatatga
    15541 accgttttaa aaaatcaaaa tatgtcatta ttgtttttgt cactgttctg cttgtgtcag
    15601 ctctcttagc gacgacttat tcaagtacaa ttgtgacaaa attaggagat ggaatctcat
    15661 tggttgatag agttgtacaa aaaccttttc agtggtttga ttctgtcaaa tcagatttgg
    15721 ctcatttgac acgaacatat aatgaaaatg aaagtttgaa gaaacagctt taccaattag
    15781 aagttaaatc aaatgaggtg gaaagtttaa agacagaaaa tgaacaactg cgccaattgc
    15841 ttgatatgaa gtctaaattg caagccacaa agactttagc agcagatgtt attatgcgtt
    15901 ctccggtatc ttggaagcag gagttgacct tagatgcagg tagatcaaaa ggtgcttctg
    15961 agaacatgtt agctattgca aatggtggct tgattgggag tgtttcaaaa gtagaggaga
    16021 actctactat agtcaacctt ctgacaaata cggaaaatgc tgataagatt tctgttaaaa
    16081 ttcaacatgg ctctactaca atttatggaa ttattattgg ctatgacaag gaaaatgacg
    16141 ttcttaaagt tagccaatta aatagtaata gcgatattag tgcgggagat aaggtgacta
    16201 ctggtggatt aggaaacttt aacgttgctg atattcctgt tggtgaagtg gttgccacaa
    16261 cgcatagtac agactatttg acacgagaag taactgttaa attgagtgca gatactcata
    16321 atgtagatgt gatagaatta gtggggaatt cataatgaga cagttgaagc gagttggagt
    16381 atttttattg cttcctttct ttgttctaat tgacgcccat attagccagc ttctgggctc
    16441 atttttcccc catgtacatt tggctagtca ttttcttttt ctatttctct tatttgagac
    16501 gatagaagta tcagagtatc tctacctagt ctattgtttt gttataggct tggtttatga
    16561 tgtttacttt ttccatctaa tagggattac aactctctta tttatcttat tgggagcctt
    16621 ccttcataaa ttgaatagtg ttattttgtt gaatcgttgg acaagaatgc tagctatgat
    16681 tgtactgaca ttcctgtttg aaatgggtag ttatcttttg gcttttatgg tagggttgac
    16741 agtagatagc atgtcgattt ttatagtcta tagcttggta ccgacgatga ttttaaattt
    16801 tttatggatt actgtttttc aatttatttt tgaaaaatat tatctataag aacgacatat
    16861 aaatgtaaca aaggcgtaat atttattagg cctttttttg gtatactagt attgtcttta
    16921 aaagaaggag tatctacgta atatgaagaa aaaaatctta gcgtcacttt tattaagtac
    16981 agtaatggtt tctcaagtag ctgttttaac aactgcgcat gcagaaacga ctgatgacaa
    17041 aattgctgct caagataata aaattagtaa cttaacagca caacaacaag aagcccaaaa
    17101 acaagttgac caaattcagg agcaagtatc agctattcaa gctgagcagt ctaacttgca
    17161 agctgaaaat gatagattac aagcagaatc taagaaactc gagggtgaga ttacagaact
    17221 ttctaaaaac attgtttctc gtaaccaatc gttggaaaaa caagctcgta gtgctcaaac
    17281 aaatggagcc gtaactagct atatcaatac cattgtaaac tcaaaatcaa ttacagaagc
    17341 tatttcacgt gttgctgcaa tgagtgaaat cgtatctgca aacaacaaaa tgttagaaca
    17401 acaaaaggca gataaaaaag ctatttctga aaaacaagta gcaaataatg atgctatcaa
    17461 tactgtaatt gctaatcaac aaaaattggc tgatgatgct caagcattga ctacgaaaca
    17521 ggcagaacta aaagctgctg aattaagtct tgctgctgag aaagcgacag ctgaagggga
    17581 aaaagcaagt ctattagagc aaaaagcagc agctgaggca gaggctcgtg cagctgcggt
    17641 ggcagaagca gcttataaag aaaaacgagc tagccaacaa caatcagtac ttgcttcagc
    17701 aaacactaac ttaacagctc aagtgcaagc agtatctgaa tctgcagcag cacctgtccg
    17761 tgcaaaagtt cgtccaacat acagtacaaa cgcttcaagt tatccaattg gagaatgtac
    17821 atggggagta aaaacattgg caccttgggc tggagactac tggggtaatg gagcacagtg
    17881 ggctacaagt gcagcagcag caggtttccg tacaggttca acacctcaag ttggagcaat
    17941 tgcatgttgg aatgatggtg gatatggtca cgtagcggtt gttacagctg ttgaatcaac
    18001 aacacgtatc caagtatcag aatcaaatta tgcaggtaat cgtacaattg gaaatcaccg
    18061 tggatggttc aatccaacaa caacttctga aggttttgtt acatatattt atgcagatta
    18121 atttacagag ggactcgaat agagccctct tttcaggttt taccgtgaca atccctatta
    18181 aaaattatat caaaatagct tgaaaatatt ggaaaagtat ggtaaaatga aaattgtcgt
    18241 gtgaacgata atactcattc ttgatgaatt gtgaagcagt tgcccttggg tcgttttgcg
    18301 agttgaagtc aagaagagga aaaaaacaaa aaggagaaat actcatggca gtaatttcaa
    18361 tgaaacaact tcttgaggct ggtgtacact ttggtcacca aactcgtcgc tggaatccta
    18421 agatggctaa gtacatcttt actgaacgta acggaatcca tgttatcgac ttgcaacaaa
    18481 ctgtaaaata cgctgaccaa gcatacgact tcatgcgtga tgcagcagct aacgatgcag
    18541 ttgtattgtt cgttggtact aagaaacaag cagctgatgc agttgctgaa gaagcagtac
    18601 gttcaggtca atacttcatc aaccaccgtt ggttgggtgg aactcttaca aactggggaa
    18661 caatccaaaa acgtatcgct cgtttgaaag aaattaaacg tatggaagaa gatggaactt
    18721 tcgaagttct tcctaagaaa gaagttgcac ttcttaacaa acaacgtgcg cgtcttgaaa
    18781 aattcttggg cggtatcgaa gatatgcctc gtatcccaga tgtgatgtac gtagttgacc
    18841 cacataaaga gcaaatcgct gttaaagaag ctaaaaaatt gggaatccca gttgtagcga
    18901 tggttgacac caatactgat ccagatgata tcgatgtaat catcccagct aacgatgacg
    18961 ctatccgtgc tgttaaattg atcacagcta aattggctga cgctattatc gaaggacgtc
    19021 aaggtgagga tgcagtagca gttgaagcag aatttgcagc ttcagaaact caagcagatt
    19081 caattgaaga aatcgttgaa gttgtagaag gcgacaacgc ttaatttata caaatagtaa
    19141 ttacctagga gggcggggct tagcccggct ctcctatttt caaaaaatat aggagaatta
    19201 aaatggcaga aattacagct aaacttgtaa aagagttgcg tgaaaaatct ggtgccggtg
    19261 ttatggacgc taaaaaagcg cttgtagaaa cagacggtga catcgaaaaa gcgattgaat
    19321 tgcttcgtga aaaaggtatg gctaaggcag ctaagaaagc tgaccgtgtt gctgcagaag
    19381 gtttgactgg tgtttatgtt aacggtaatg ttgcagcagt tattgaagta aacgctgaaa
    19441 ctgacttcgt tgcaaaaaac gctcaattcg ttgaattggt aaatactaca gctaaagtca
    19501 ttgctgaagg aaaacctgct aacaacgaag aagctcttgc tttgataatg ccttcaggtg
    19561 aaactcttga agctgcatac gtatctgcaa cagcaactat cggagagaaa atctcattcc
    19621 gtcgctttgc attgattgaa aaaacagacg cacaacactt tggagcatac caacataacg
    19681 gtggacgtat cggtgttatt tcagttgttg aaggtggaga cgaagcactt gctaaacaat
    19741 tgtcaatgca catcgcagcg atgaaaccaa cagttctttc ttacaaagaa ttggatgagc
    19801 aattcgttaa agatgagttg gcacaattga atcacgttat cgaccaagac aacgaaagcc
    19861 gtgcaatggt taataaacca gctcttccac acttgaagta tggatcaaaa gctcaattaa
    19921 ctgatgatgt tattgctcaa gctgaagctg acatcaaagc tgaattggct gcagaaggca
    19981 aaccagaaaa aatctgggac aaaattattc caggtaaaat ggatcgcttc atgcttgata
    20041 acactaaagt tgaccaagct tacacacttc ttgcacaagt ttacatcatg gatgacagca
    20101 agacagttga agcatacctt gaatcagtta acgcttcggt agttgagttt gctcgctttg
    20161 aagttggtga aggtatcgag aaagctgcaa acgacttcga agctgaagtt gcagctacaa
    20221 tggcagcagc cttgaataac taataataga aaggaagcaa attgcttcct tttttgtata
    20281 gggcttagat ttttcctgaa attgtttttt ccccagtctc actcgattag aataattaaa
    20341 acaaaaagcc ctataataag ggctaagtgc atttaggaga ctatacttca aattcatagt
    20401 aagcgcgttc ataacaaggt atctatccta attccccaac ttccagtata ctagataaaa
    20461 aataattatc ggagtaaagg tattgtacta tgataattca actaagtgat ttaggtcaag
    20521 ttcaccttgt ttgtggcaag acagatatga ggtagggaat agactcatta gcctatgtag
    20581 ttaaaaccca ctttgaattg gattctttct ccggtcaagt ctttctcttt tgtggtgaac
    20641 gtaaagaccg ctttaaagcc ctttactggg atggtcaagg attttggcta ctatataaac
    20701 gctttgagaa cggaaaactg acttggctaa gtacagaaaa ggatgtcaaa gctctcgcac
    20761 ctgaacaagt agattggctt atgaagggct tttctatcac tccaaaatat agtatattga
    20821 aactagaata gtacacctct gcttctaaaa cattgttaga aatcgatttg actgtcctga
    20881 tcgatttatc ctgttcttat ttcattttac tataaatcta tcagaaagtt gtgatttcta
    20941 ttgaaatgag gactttcttt ttacactcat ctgctttcaa aaagcattct agtccgtctc
    21001 cgattaacga tggactttat cacctccttc tccagtcctt gtatgacatc ttccaaagtt
    21061 cgaaaggctt tattcttaaa tccacgttta cgaatctctt tccacacttg ttcaatgggg
    21121 ttcatctctg gtgtgtatgg aggaataaat gcaaagccaa tattagtcgg aatctttaag
    21181 gtacttgatt tatgccatat agcattgtcc ataacgagta aaagataatc atctggataa
    21241 gcttgtgaaa tctcctattc ctaaagcccc tttagcgcat aactttggct cagcttctat
    21301 tatcgctcac accatccatc agaagtttaa tctgaaggta cccaattatc gccaagaaga
    21361 agattgggct aggatgggtt taccaatcat acgtaaggaa atctctaatt ggcatatcaa
    21421 gacgagtcaa tactatttgg agccccttta taacctcttg cgagagagac tattgactca
    21481 gcccttactt catgcggatg aaacttctta tagggtgcta gagagtgata gtcagctgac
    21541 ttactactgg acttttttgt ctgggaaagc tgagaaacaa gggattacgc tttaccatca
    21601 tgtcctaatc gatttgttca tatcttattt caatccatta taaatagtga gaaatatcta
    21661 tcctatcttc tagaatgtct tccaaacgtg gaaactctcg taaacaaaga ggttttagag
    21721 gcctatttac catggactaa agttgtacaa gaaaagtgca aataagaaat ctccagatta
    21781 ggaactatcc gtgagttctc tagtctggag atttttcaat agacttcgtt attggacggt
    21841 tacaattcat aaagtgctgt agagagataa cgttcaccgt tatctggtgc tagggcaagg
    21901 acttttttac ctgtacctaa ttttttggca acctcgatgg ctccgtagat agctgcagct
    21961 gaggaaatcc ctacaaggaa gccttctttt ccaccaattt cacgtccgag tgcaagagcg
    22021 tcatctgatg ttacacgaac gataccatca taggctttag tatcaagtgt atcaggaata
    22081 aatccagctg agataccttg aattttgtga ggaccaggtt tttcaccaga tagaatagca
    22141 gattcatctg cttctactgc aaaaacttga atgttagaat tttctgattt gagtgcatga
    22201 gaaacaccag aaatcgttcc accagtacct actccagcaa caaaggcatc taatccatct
    22261 ttaccgaaag cagctagtat ctcagctcct gttgttcttt cgtgtacttc tggattagct
    22321 ggattgtcaa attgaagagg aaggaaacca tcacgttcag cagcgatttc ttgagcctta
    22381 gcaatagcac ctttcattcc ctcgctacca ggagttagga cgagttcagc accataagct
    22441 tggataattt tacgtcgttc tacactcata gtttcaggca taacgatgac gactttatac
    22501 cctttagcag cacctaccca tgaaagtcca ataccggtgt ttccacttgt tgcttcaaca
    22561 atagtagaac caagtttcag aataccatct tgttcagctt tttcaatcat gctaagggca
    22621 atacggtctt ttacagatga accaggatta aatgcttcaa gctttatata gacgtctgca
    22681 gcaccttctg gcacgatgtt gttaagttta acaatcggtg tttgaccgat taattcagta
    22741 atgttgttat aaatagacat atgaatacct ctttgtataa gatatcttta gtataacgct
    22801 atccatacca tttgtaaaat atagaaatcc tatcgaagcg ataggatttc tatatatcat
    22861 agatcaaagt cattaatttt caagtgattg cgttcagtta attctagtaa ataggtgtga
    22921 aggaaaacga tatcaggttt cgtttgaatt gcttcatttt ctagtatatt ttggtacgca
    22981 tctttttatt ttgctcgcta aagtagttgt gagaatgtca acgaaacgct gtattattca
    23041 ttgtttacac taagtttaca gaaacttcaa cctctcgtaa accttggtca gttaaagtga
    23101 cttttccatt aaaaaactcc acaagtgcag ctttaatagt ttctttttct tctttatcaa
    23161 cataaatcat cgtatcgact tgatctgtaa agtttgtatc cagctccatg agatcatgtt
    23221 ctttaaggaa gttactgtac tcttggtact gagcataaga catttgaata gcaatgccag
    23281 cctgttcttt tatttcaata ataccaattt ctttgacagc taaggcgaca ctgccggcgt
    23341 aagcacgaat tagtcctcca gcgcctagtt taataccacc aaagtagcgt gtcacgacca
    23401 cacagacatt ggtgagattg tgattttcta gtaccccaag catgggaaca ccagcagtac
    23461 cactaggctc accatcatca cttgtacgtt taatttcact acgttctcca ataataaagg
    23521 cagagcagtt atgtgtcgct ttgtagtgtt cttttttgat ggcagtaatg aagtcacgag
    23581 cctcttcttc gctataaaca cgcttggcat ggcagataaa gcgagatttt ttgatttctt
    23641 cttggacttg gccgtcctct ttaattgtcc taaattccat gatttaattg tactaaaaaa
    23701 taatctaaag tgctagtttt tacgaataaa gaagtatgaa agtaaattta gattatctcg
    23761 gtcgtttatt tactgagaat gaattaacag aagaagaacg tcagttggcg gagaaacttc
    23821 cagcaatgag aaaggagaag gggaaacttt tctgtcaacg ttgtaatagt actattctag
    23881 aagaatggta tttgcccatc ggtgcttact attgtcgaga gtgcttgctg atgaagcgag
    23941 tcagaagtga tcaaacttta tactattttc cgcaggagga ttttccgaag caagatgttc
    24001 tcaaatggcg cggccaatta actccttttc aagagaaggt gtcagaggga ctgcttcaag
    24061 cagtagacaa gcaaaagcca accttagttc atgcggtaac aggagctgga aagacagaaa
    24121 tgatttatca agtagtggct aaagtgatca atgcgggtgg tgcagtgtgt ttggctagtc
    24181 ctcgcataga tgtttgtttg gagctgtaca agcgcctgca acaggatttt tcttgcggta
    24241 tagctttgct acatggagaa tcggaacctt attttcgaac accactagtt gttgcaacaa
    24301 cccatcagtt attgaagttt tatcaagctt ttgatttgct gatagtggat gaagtagatg
    24361 cttttcctta tgttgataat cccacgcttt accacgctgt caagaatagt gtaaaggaga
    24421 atggattgag aatcttttta acagcgactt cgaccaatga gttagataaa aaggtccgtt
    24481 taggagaact aaaaagactg agtttaccga gacggtttca tggaaatccg ttgattattc
    24541 caaaaccaat ttggttatcg gattttaatc gctacttaga caagaatcgt ttgtcaccaa
    24601 agttaaagtc ctatattgag aagcagagaa agacagctta tccgttactc atttttgctt
    24661 cagaaattaa gaaaggggag cagttagaag aaatcttaca ggagcaattt ccaaatgaga
    24721 aaattggctt tgtatcttct gtaacagagg atcgattaga gcaagtacaa gcttttcgag
    24781 atggagaact gacaatactt atcagtacga caatcttgga gcgtggagtt accttccctt
    24841 gtgtggatgt tttcgtaata gaggccaatc atcgtttgtt taccaagtct agtttgattc
    24901 agattggtgg acgagttgga cgaagcatgg atagaccgac aggagatttg cttttcttcc
    24961 atgatgggtt aaatgcttca atcaagaagg cgattaagga aattcagatg atgaataagg
    25021 aggctggtct atgaagtgct tgttatgtgg gcagactatg aagactgttt taacttttag
    25081 tagtctctta cttctgagga atgatgactc ttgtctttgt tcagactgtg attctacttt
    25141 tgaaagaatt ggggaagaga actgtccaaa ttgtatgaaa acagagttgt caacaaagtg
    25201 tcaagcttgt caattttggt gtaaagaagg agttgaagtc agtcatagag cgatttttac
    25261 ttacaatcaa gctatgaagg attttttcag tcggtataag tttgatggag acttcctgtt
    25321 aagaaaagtt ttcgcttcat ttttaagtga ggagttgaaa aagtacaaag agtatcaatt
    25381 tgttgtaatt cccctaagtc ctgatagata tgctaacaga ggatttaatc aggttgaggg
    25441 cttggtagag gcagcaggct ttgagtatct agatttatta gagaaaagag aagagagagc
    25501 cagttcttct aaaaatcgtt cagagcgctt ggggacagaa cttcctttct ttattaaaag
    25561 tggagtcact attcctaaaa aaatcctact tatagatgat atctatacta caggagcaac
    25621 tataaatcgt gttaagaaac tgttggaaga agctggtgct aaggatgtaa aaacattttc
    25681 ccttgtaaga tgaggaaaaa atttgcaaaa atagaatgaa agcgttataa taaaatcata
    25741 aaaacaaaaa tgtttagaaa gaaggtactc atatgattaa atatagtatc cgtggtgaaa
    25801 acctagaagt aacagaagca attcgtgatt atgtagtttc taaactcgaa aagatcgaaa
    25861 agtacttcca accagaacaa gagttggatg cccgaattaa cttaaaagtt tatcgtgaaa
    25921 aaacggctaa agtggaagta acgattccgc ttggatctat tactctccgc gcagaagatg
    25981 tatctcaaga tatgtatggt tcaattgacc ttgtaactga taaaattgaa cgtcagattc
    26041 gtaaaaataa aacaaaaatc gagcgtaaaa ataaaaataa ggtagcaact ggtcaattat
    26101 ttacagatgc tttggtggaa gattcaaata ttgtccagtc taaagttgtt cgttcaaaac
    26161 aaattgattt aaaaccaatg gatttggaag aagcaattct acaaatggat ttattggggc
    26221 atgatttctt tatctatgtg gatgttgaag atcagacaac caatgtgatt tatcgtcgtg
    26281 aggatggcga aattggtttg ttagaggtta aagaatctta atttcaatat gtgtaaaggt
    26341 aggtttactg aattgtaaac tacctttttc ttataattga tagaattact gattacactt
    26401 ttaaaaagtc gctttttgag gggtattatg gtataatggg tgccaagagg ttttgaatga
    26461 aaaaatttta tgtaagtcca atttttccta ttctagtagg attgattgcg tttggagtct
    26521 tatccacttt cattattttt gttaataata atctgttgac ggttttaatt ttgtttcttt
    26581 ttgtaggagg ctatgttttt ttatttaaga aactgagagt gcattataca aggagtgatg
    26641 tagaacagat acagtatgta aaccaccaag cggaagaaag tttgacagct ctattggaac
    26701 agatgcctgt aggtgttatg aaattgaatt tatcttctgg agaggttgag tggtttaatc
    26761 cctatgctga attgattttg accaaggaag atggtgattt tgatttagaa gctgttcaaa
    26821 cgattatcaa ggcttcagta ggaaatccgt ctacttatgc caagcttggt gagaagcgtt
    26881 atgctgttca tatggatgct tcttccggtg ttttgtattt tgtagatgta tccagggaac
    26941 aagccataac agatgaattg gtaacaagtc gaccagtgat tgggattgtc tctgtggata
    27001 attatgatga tttggaggat gcaacttctg agtcagatat tagtcaaatc aatagttttg
    27061 tagctaattt tatatcagag ttttcagaaa aatacatgat gttttctcgt cgggtaagta
    27121 tggatcgatt ttatctattt actgactaca cggtgcttga gggcttgatg aatgataaat
    27181 tttctgttat tgatgctttc agagaagagt cgaaacagag acagttgccc ttgaccttaa
    27241 gtatgggatt ttcttatggc gatggaaatc atgatgagat agggaaagtt gctttgctca
    27301 atttgaactt ggctgaagta cgtggtggcg accaggtggt tgttaaggaa aacgacgaaa
    27361 cgaaaaatcc agtttatttt ggtggtgggt ctgctgcttc aatcaagcgt acacggactc
    27421 gtacgcgcgc tatgatgaca gctatttcag ataagattcg gagtgtagat caggtttttg
    27481 tagtcggtca caaaaattta gacatggatg ctttgggctc tgctgtaggt atgcagttgt
    27541 tcgccagcaa tgtgattgaa aatagctatg ctctttatga tgaagaacaa atgtctccag
    27601 atattgaacg agctgtttca ttcatagaaa aagaaggagt tacgaagttg ttgtctgtta
    27661 aggatgcatt ggggatggtg accaatcgtt ctttgttgat tcttgtagac cattcaaaga
    27721 cagccttaac attatcaaaa gaattttatg atttatttac ccaaaccatt gttattgacc
    27781 accatagaag ggatcaggat tttccagata atacggtcat tacttatatc gaaagtggtg
    27841 caagtagtgc cagtgagttg gtaacggaat tgattcagtt ccagaattct aagaaaaatc
    27901 gtttgagtcg tatgcaagca agtgtcttga tggctggtat gatgttggat actaaaaatt
    27961 tcacctcgcg agtaactagt cggacatttg atgttgctag ctatctcaga acgcgcggaa
    28021 gtgatagtat tgctatccag gaaatcgctg cgacagattt tgaagaatat cgtgaggtca
    28081 atgaacttat tttacagggg cgtaaattag gttcagatgt actaatagca gaggctaagg
    28141 acacgaaatg ctatgataca gttgttatta gtaaggcagc agatgccatg ttagccatgt
    28201 caggtattga agcgagtttt gttcttgcga agaatacaca aggatttatc tctatctcag
    28261 ctcgaagtcg tagtaaactg aatgtacaac ggattatgga agagttaggc ggtggaggcc
    28321 actttaattt ggcagcagct caaattaaag atgtaacctt gtcagaagca ggtgaaaaac
    28381 tgacagaaat tgtattaaat gaaataaagg aaaaggagaa agaagaatga aagtaatctt
    28441 tttagcagat gttaaaggaa aaggtaaaaa aggcgaaatt aaggaagtac caacagggta
    28501 tgcgcaaaac tttcttatca aaaagaatct agccaaagaa gcgactgctc aagctgtagg
    28561 tgaacttcgt ggtaaacaaa aatcggaaga aaaagctcac gctgagatga ttgcagaagg
    28621 aaaagcaatt aaagcacaac ttgaagcaga agaaactgtt gtagaatttg ttgaaaaagt
    28681 tggtccagat ggtcgtacct ttggctctat taccaataag aagattgcag aagaattgca
    28741 aaaacaattt ggaattaaga ttgataaacg tcatattcaa gtacaagctc cgattcgagc
    28801 ggttggtttg attgatgtgc cagtgaaaat ctatcaagat atcacaagtg taatcaatct
    28861 tcgtgtgaaa gaaggataag tttacacctt cttgacaaga ttgtaaaagg aagggaagtc
    28921 tgatggcaga agtagaagag ttacgagtac aacctcaaga tatcttagct gagcaatccg
    28981 ttttaggggc tatctttatt gatgagagta aacttgtttt tgtgcgagaa tacattgagt
    29041 ctcgggactt ttttaagtat gcccatcgtt tgattttcca agccatggtc gatttatccg
    29101 atcgtggtga tgccatagat gcaacaacgg ttcgtactat ccttgataat caaggtgatt
    29161 tacagaatat tggtggcttg tcttacttgg ttgagattgt taattctgtg ccaacttctg
    29221 ctaatgcgga gtattatgct aagattgttg cagaaaaagc aatgctacgt cgtttaattg
    29281 ccaagttgac agagtctgtc aaccaagctt acgaagcgtc acaaccagct gatgaaatta
    29341 ttgctcaggc agaaaaaggg ctgattgatg tcagtgaaaa tgcaaatcga agcgggttta
    29401 agaacattcg agatgtgttg aatctcaact ttggaaatct ggaagctcgc tcgcaacaaa
    29461 cgaccgatat tacaggtatt gcgacaggtt atcgtgattt ggatcatatg acaacaggac
    29521 ttcatgagga ggagttgatt atcttagcag ctcgtccagc agttggtaag acagcatttg
    29581 ccttgaatat cgctcagaac attgggacta agttggacaa aacggttgct attttttcac
    29641 tcgaaatggg tgcggaaagc ttggtagatc gtatgttagc tgcagaaggc ttagtggagt
    29701 cacattctat ccgtacaggg caattgacag atgaggagtg gcaaaaatat actattgctc
    29761 agggtaatct agctaacgcc agtatctata tcgatgatac gccaggtatt cggattacag
    29821 agattcgttc tcgttctcgt aaattggctc aagaaactgg aaatcttggt ttgattttga
    29881 tagactattt gcaacttatc acgggaactg gtcgagaaaa tcgtcaacaa gaagtttctg
    29941 aaatttctcg tcagttgaaa atactagcca aggaattgaa ggttccagta atcgctctga
    30001 gtcagctttc tcgtggtgta gaacaacgtc aggacaagag accggtcttg tctgatattc
    30061 gtgaatctgg gtctattgag caggacgctg atatcgtagc ttttctctat cgcgatgact
    30121 actatgaacg tggtggtgaa gaagaggagg gtatcccaaa taataaggtg gaagttatta
    30181 tcgagaaaaa ccgtagtgga gctcgtggaa cagtggaatt gattttccaa aaagaataca
    30241 ataaattttc aagtatctca aagagggagg cataagatgt cagatgcatt tacagatgta
    30301 gccaagatga aaaaaatcaa agaagaaatc aaggcacatg agggacaagt cgtagaaatg
    30361 actttggaga atggtcgtaa gcgccaaaaa aatagattgg gtaagctaat tgaagtttat
    30421 ccatctctat ttattgtgga gtttggggat gtggaaggag ataaacaagt taatgtttac
    30481 gttgaatcct ttacttactc agatattctt acagaaaaga atttgattca ttatcttgac
    30541 taaagtgaga aattttctca ctttttcttt tttctccgaa taatttaggt gaaggcaatc
    30601 atcgctttat attatttttc aaggaggaag aatgaaaatt ttaccgttta tagcaagagg
    30661 aacaagttat tacttgaaga tgtcagttaa aaagcttgtt ccttttttag tagtaggatt
    30721 gatgctagca gctggtgata gtgtctatgc ctattccaga ggaaatggat cgattgcgcg
    30781 tggggatgat tatcctgctt attataaaaa tgggagccag gagattgatc agtggcgcat
    30841 gtattctcgt cagtgtactt cttttgtagc ctttcgtttg agtaatgtca atggttttga
    30901 aattccggca gcttatggaa atgcgaatga atggggacat cgtgctcgtc gggaaggtta
    30961 tcgtgtagat aatacaccga cgattggttc cattacttgg tctactgcag gaacttatgg
    31021 tcatgttgcc tgggtgtcaa atgtaatggg agatcagatt gagattgagg aatataacta
    31081 tggttataca gaatcctata ataaacgagt tataaaagca aacacgatga caggatttat
    31141 tcattttaaa gatttggatg gtggcagtgt tgggaatagt caatcctcaa cttcaacagg
    31201 cggaactcat tattttaaga ccaagtctgc tattaaaact gaacccctag ttagtgcaac
    31261 tgtgattgat tactattatc ctggagagaa ggttcattat gatcaaattc tcgaaaaaga
    31321 cggctacaag tggttgagtt atactgccta taatggaagc tatcgttatg ttcaattgga
    31381 ggctgtgaat aaaaatcctc taggtaattc tgttctttct tcaacaggtg gaactcatta
    31441 ttttaagacc aagtctgcta tcaaaactga acccctagtt agcggaactg tgattgatta
    31501 ctattatcct ggggagaagg ttcattatga tcagatactt gaaaaagacg gctacaagtg
    31561 gttgagttat actgcctata atggaagtcg tcgctatata cagctagagg gagtgacttc
    31621 ttcacaaaat tatcagaatc aatcaggaaa tatctctagc tatggatcca ataatagttc
    31681 aactgtcggt tggaagaaaa taaatggtag ttggtatcat ttcaaatcaa atggttctaa
    31741 atcaacagga tggctgaaag acggttctag ctggtattat ttgaaattat ctggtgaaat
    31801 gcagacagga tggttaaagg agaatggctc gtggtattat ctgggtagtt caggggcaat
    31861 gaaaacaggc tggtaccagg tctctggtaa gtggtattat tcttactctt caggcgcctt
    31921 agctgttaat acgacggtgg atggctacag agtaaacagt gatggagaac gagtatagaa
    31981 aattggagta ggagaaattt cctgctcttt tctgtaagca gtttccttga catttttctt
    32041 attttgcgct atcatatata catataatat atgggagtct gtgtacagtc tgagaggaag
    32101 tgttaaactt cgaccgcacc tgatctgggt aatgccagcg gagggaacga tacttagtct
    32161 aattttgcac cttttccatg tatggtaaag gtttttcttt tttttaaaag gaaaacgaga
    32221 agaggaggtt cttatgaaag caagcattgc cttgcaagtt ttacccctag cacaggggat
    32281 tgatcggata gctgttattg atcaggtcat tgcttatctg caaactcaag aagtgacgat
    32341 ggtagtgaca ccatttgaaa cggtcttgga aggggagttt gatgagctta tgcgcattct
    32401 aaaagaagcg ctggaagtgg cagggcagga ggcagacaat gtctttgcca atgtcaaaat
    32461 aaatgtagga gagattttaa gtattgatga gaaacttgaa aagtatactg agacgacaca
    32521 ttagtctatt gggctttctc ggagtattgt caatctggca gttagcaggt tttcttaaac
    32581 ttctccccaa gtttatcctg ccgacacctc ttgaaattct ccagcccttt gttcgtgaca
    32641 gagaatttct ctggcaccat agctgggcga ccttgagagt ggctttactg gggctgattt
    32701 tgggagtttt gattgcctgt cttatggctg tgctcatgga tagtttgact tggctcaatg
    32761 acctgattta ccctatgatg gtggtcattc agaccattcc gaccattgcc atagctccta
    32821 tcctggtctt gtggctgggt tatgggattt tgcccaagat tgtcttgatt atcttaacaa
    32881 caacctttcc catcatcgtt agtattttgg acggttttag gcattgtgac aaggatatgc
    32941 tgactttgtt tagtctgatg cgagcaaacc cttggcaaat cctgtggcat tttaaaatcc
    33001 cggttagcct gccttacttt tatgcaggtc tgagggtcag tgtctcctac gcctttatca
    33061 caactgtggt atctgagtgg ttgggaggtt ttgaaggtct tggtgtttat atgattcagt
    33121 ctaaaaaact gtttcagtat gataccatgt ttgccattat tattctggtg tcgattatca
    33181 gtcttttggg tatgaagctg gtcgatatca gtgaaaaata tgtgattaaa tggaaacgta
    33241 cttaaaatag agcgtttttg aaaaagaaaa gaggaaatca aaatgaagaa aacatggaaa
    33301 gtgtttttaa cgcttgtaac agctcttgta gctgttgtgc ttgtggcctg tggtcaagga
    33361 actgcttcta aagacaacaa agaggcagaa cttaagaagg ttgactttat cctagactgg
    33421 acaccaaata ccaaccacac agggctttat gttgccaagg aaaaaggtta tttcaaagaa
    33481 gctggagtgg atgttgattt gaaattgcca ccagaagaaa gttcttctga cttggttatc
    33541 aacggaaagg caccatttgc agtgtatttc caagactaca tggctaagaa attggaaaaa
    33601 ggagcaggaa tcactgccgt tgcagctatt gttgaacaca atacatcagg aatcatctct
    33661 cgtaaatctg ataatgtaag cagtccaaaa gacttggttg gtaagaaata tgggacatgg
    33721 aatgacccaa ctgaacttgc tatgttgaaa accttggtag aatctcaagg tggagacttt
    33781 gagaaggttg aaaaagtacc aaataacgac tcaaactcaa tcacaccgat tgccaatggc
    33841 gtctttgata ctgcttggat ttactacggt tgggatggta tccttgctaa atctcaaggt
    33901 gtagatgcta acttcatgta cttgaaagac tatgtcaagg agtttgacta ctattcacca
    33961 gttatcatcg caaacaacga ctatctgaaa gataacaaag aagaagctcg caaagtcatc
    34021 caagccatca aaaaaggcta ccaatatgcc atggaacatc cagaagaagc tgcagatatt
    34081 ctcatcaaga atgcacctga actcaaggaa aaacgtgact ttgtcatcga atctcaaaaa
    34141 tacttgtcaa aagaatacgc aagcgacaag gaaaaatggg gtcaatttga cgcagctcgc
    34201 tggaatgctt tctataaatg ggataaagaa aatggtatcc ttaaagaaga cttgacagac
    34261 aaaggcttca ccaacgaatt tgtgaaataa tgacagaaat tagactagag catgtcagtt
    34321 atgcctatgg tcaggagagg attttagagg atatcaacct acaggtgact tcaggcgaag
    34381 tggtttccat tctaggccca agtggtgttg gaaagaccac cctctttaat ctaatcgctg
    34441 ggattttaga agttcagtca gggagaattg tccttgatgg tgaagaaaat cccaaggggc
    34501 gcgtgagtta tatgttgcaa aaggatctgc tcttggagca caagacggtg cttggaaata
    34561 tcattctgcc cctcttgatt caaaaggtgg ataaggcaga agctatttcc cgagcggata
    34621 aaattcttgc gaccttccag ctgacagctg taagagacaa gtatcctcat gaacttagcg
    34681 gtgggatgcg ccagcgtgta gccttactcc ggacctacct ttttgggcac aagctctttc
    34741 tcttagatga ggcctttagc gccttggatg agatgacaaa gatggaactc cacgcttggt
    34801 atcttgagat tcacaaacag ttgcagctaa caaccctgat catcacgcat agtattgagg
    34861 aggccctcaa tctcagcgac cgtatctata tcttgaaaaa tcgccctggg cagattgttt
    34921 cagaaattaa actagattgg tctgaagatg aggacaagga agtccaaaag attgcctaca
    34981 aacgtcaaat cttggcagaa ttaggcttag ataagtagaa aaatagggag ttggtgaaga
    35041 ttatccttta ccagcgccct ttttctttta aaaatgagaa aatttcggta taatagtcaa
    35101 acaaggtcaa ggtttaaaga gagaggtggg tttgttatga gatttaaaaa tacatcggat
    35161 catattgagg cctacatcaa ggcgatttta gatcaatctg gtatcgtgga gttgcaacgg
    35221 agtcagttgg cagatacctt tcaggttgtt cctagtcaga ttaactacgt gatcaagaca
    35281 cgctttacgg aaagtagagg ctacttggtt gaaagtaagc gtggtggcgg aggctacatt
    35341 cgtataggac ggattgagtt ttctagtcat catgaaatgc tccgggagct gctttactcg
    35401 attggtgagc gagtcagtca agaaatttat gaggatattc tccagctttt ggttgagcag
    35461 gaattgatga ccaagcagga gatgaatttg ctagaatcag tagctttgga tcgcgtttta
    35521 ggagaagaag ctccagttgt tcgagcaaac atgctacgtc aggtcataca agaggtagat
    35581 agaaaaggga agtaagatga actattcaaa agcattgaat gaatgtatcg aaagtgccta
    35641 catggttgct ggacattttg gagctcgtta tctagagtcg tggcacttgt tgattgccat
    35701 gtctaatcac agttatagtg tagcaggggc aactttaaat gattatccgt atgagatgga
    35761 ccgtttagaa gaggtggctt tggaactgac tgaaacggac tatagccagg atgaaacctt
    35821 tatggaattg ccgttctccc gtcgtttgca ggttcttttt gatgaagcag agtatgtagc
    35881 gtcagtggtc catgctaagg tactagggac agagcacgtc ctctatgcga ttttgcatga
    35941 tggcaatgcc ttggcgactc gtatcttgga gagggctggt ttttcttatg aagacaagaa
    36001 agatcaggtc aagattgctg ctcttcgtcg aaatttagaa gaacgggcag gctggactcg
    36061 tgaagatctc aaggctttac gccaacgcca tcgtactgtg gctgacaaac aaaattctat
    36121 ggccaatatg atgggcatgc cgcagactcc tagtggtggt ctcgaggact atacgcatga
    36181 tttgacagag caagcgcgtt ctggcaagtt agaaccagtc atcggtcggg acaaggaaat
    36241 ctcacgtatg attcaaatct tgagccgtaa gactaagaac aaccctgtct tggttgggga
    36301 tgctggtgtc gggaaaacag ctctggtgct tggtcttgct cagcgtattg ccagtggtga
    36361 tgtgcctgcg gaaatggcta agatgcgcgt gttagaactt gatttgatga atgtcgttgc
    36421 agggacacgc ttccgtggtg actttgaaga acgcatgaat aatatcatca aggatattga
    36481 agaagatggc caagtcatcc tctttatcga tgaactccac accatcatgg gttctggtag
    36541 cgggattgat tcgactctgg atgcggccaa tatcttgaaa ccagccttgg cgcgtggagc
    36601 tttgagaacg gttggtgcca ccactcagga agaatatcaa aaacatatcg aaaaagatgc
    36661 ggctctttct cgtcgtttcg ctaaagtgac gattgaagaa ccaagtgtgg cagatagtat
    36721 gactatttta caaggtttga aggcgactta tgagaaacat caccgtgtac aaatcacaga
    36781 tgaagcggtt gaaacagcgg ttaagatggc tcatcgttat ttaaccagtc gtcacttgcc
    36841 agactctgct atcgacctct tggacgaagc agcagcaaca gtgcaaaata aggtaaagta
    36901 tgtaaaagca gacgattcag atttgagtcc agctgataag gccctgatgg atggcaagtg
    36961 gaaacaggca gcccagctaa tcgcaaaaga agaggaagta cctgtctaca aagacttggt
    37021 gacagagtct gatattttga ccaccttgag tcgcttgtca ggaatcccag ttcaaaaact
    37081 gactcaaacg gatgctaaga agtatctaaa tcttgaagca gaactccata aacgtgtcat
    37141 cggtcaagat caagctgttt caagcattag tcgtgcgatt cgccgtaatc agtcaggaat
    37201 tcgcagtcac aagcgtccga tcggttcctt tatgttccta ggacccacag gtgtcggtaa
    37261 gaccgagttg gccaaggctt tggcagaagt tctctttgat gacgaatcag cccttatccg
    37321 ctttgatatg agtgagtata tggagaaatt cgcagccagc cgtctaaatg gagctcctcc
    37381 aggctatgtg ggttacgaag aaggtggaga gttgacagag aaggttcgca ataaacccta
    37441 ttccgttctc ctctttgatg aggtagagaa ggcccaccca gatattttta atgttctctt
    37501 gcaggttctg gatgacggtg ttttgacaga tagcaaggga cgcaaggtcg atttttcaaa
    37561 taccattatc attatgacat cgaacctagg tgcgactgcc cttcgtgatg ataagactgt
    37621 tggttttggg gctaaggata ttcgttttga ccaggaaaat atggaaaaac gcatgtttga
    37681 agaactgaaa aaagcttata gaccggaatt catcaaccgt attgatgaga aggtggtctt
    37741 ccatagccta tctagtgacc atatgcagga agtggtgaag attatggtca agcctttagt
    37801 ggcaagtttg gctgaaaaag gcattgactt gaaattacaa gcttcagctc tgaaattgtt
    37861 agcaaatcaa ggatatgacc cagagatggg agctcgccca cttcgcagaa ccctgcaaac
    37921 agaagtggag gacaagttgg cagaacttct tcttaaggga gatttagtgg caggcagcac
    37981 acttaagatt ggtgtcaaag caggccagtt aaaatttgat attgcataaa agaataaaag
    38041 tatcagcatc tgaccataag tcacagtgga gtgaaactca atgaaaatca aagagcaaac
    38101 taggcagcta gccgcaggtt gctcaaaaca ctggtttgag gttgcagata gagctgacgt
    38161 ggtttgaaga gattttcgaa gagtatgaaa ctaaaaccta tagcttctaa acgatccgtg
    38221 gttttcacca ttcaacacaa aattcatatg tttattaccc tccgtcgtat ttgtcttaga
    38281 gcgtgtgtag tagaaaaaga gcagtcttat ctgaaatttt tattctttca aaagagacct
    38341 gtttcttttt tgcatgtcaa atccgttcta gctggtattt gaaaaatcaa actaatattc
    38401 aatgaaaatc aaagaacaaa ctaggcagct agccacaggt tgctcaaaac actgttttga
    38461 ggttgtagat agagctgacg tagtttgaag agattttcga agagtataag ctgcaagatg
    38521 aatgattttc ttgtattgac gttgttgttg acaaaaagta gcggataaat gaaatccatt
    38581 ccattatcat agatgatagg ctggtaggaa attttcaaat agcatacagg aaatagatgt
    38641 atggagttct ggtagtagaa agggagagag atgaacattt tagttgcaga tgacgaggaa
    38701 atgattagag aaggaattgc agcatttctg acagaagagg gttatcatgt cattatggct
    38761 aaggatggac aagaggtctt ggaaaaattt caagatctcc ctatccatct catggtattg
    38821 gatttaatga tgcctaggaa gagtggtttt gaagtgttaa aagaaatcaa tcaaaagcac
    38881 gatattcctg tcatcgtctt gagtgctctg ggagatgaaa ctactcagtc acaggtattt
    38941 gatctctatg ctgatgatca tgtgacaaaa cctttttctt tggtactgct tgtcaagcgt
    39001 attaaggcgc ttatcagacg ttactacgtc atagaggata tttggcgata tcaggatgta
    39061 acagtggatt ttacctctta caaagcacat tataaaaatg aagaaattga tctcaaacca
    39121 aaggaattac tggtactaaa gtgtttgatt cagcataaaa atcaagtttt aagtagagag
    39181 cagatattgg aagaaatttc aaaagatgta gctgatttac cttgtgatag ggtcgttgat
    39241 gtctatattc gtactcttcg caaaaaatta gctttagatt gtatcgtgac tgtgaaaaat
    39301 gttgggtata agattagctt atgataaaaa atcctaaatt attaaccaag tcttttttaa
    39361 gaagttttgc aattctaggt ggtgttggtc tagtcattca tatagctatt tatttgacct
    39421 ttccttttta ttatattcaa ctggaggggg aaaagtttaa tgagagcgca agagtgttta
    39481 cggagtattt aaagactaag acatctgatg aaattccaag cttactccag tcttattcaa
    39541 agtccttgac catatctgct caccttaaaa gagatattgt agataagcga ctccctcttg
    39601 tgcatgactt ggatattaaa gatggaaagc tatcaaatta tatcgtgatg ttagatatgt
    39661 ctgttagtac agcagatggt aaacaggtaa cagtgcaatt tgttcacggg gtggatgtct
    39721 acaaagaagc aaagaatatt ttgtttttgt atcttccata tacatttttg gttacaattg
    39781 ctttttcctt tgttttttct tatttttata ctaaacgctt gctcaatcct cttttttaca
    39841 tttcagaagt gactagtaaa atgcaagatt tggatgacaa tattcgtttt gatgaaagta
    39901 ggaaagatga agttggtgaa gttggagaag ttggaaaaca gattaatggt atgtatgagc
    39961 acttgttgaa ggttattcat gagttggaaa gtcgtaatga gcaaattgta aaattgcaaa
    40021 atcaaaaggt ttcctttgtc cgtggagcat cacatgagtt gaaaacccct ttagccagtc
    40081 ttagaattat cctagagaat atgcagcata atattggaga ttacaaagat catccaaaat
    40141 atattgcaaa gagtataaat aagattgacc agatgagcca cttattggaa gaagtactgg
    40201 agtcttctaa attccaagag tggacagagt gtcgtgagac cttgactgtt aagccagttt
    40261 tagtagatat tttatcacgt tatcaagaat tagctcattc aataggtgtt acaattgaaa
    40321 atcaattgac agatgctacc agggtcgtca tgagtcttag ggcattggat aaggttttga
    40381 caaacctgat tagtaatgca attaaatatt cagataaaaa tgggcgcgta atcatatccg
    40441 agcaagatgg ctatctctct atcaaaaata catgtgcgcc tctaagtgac caagaactag
    40501 aacatttatt tgatatattc tatcattctc aaatcgtgac agataaggat gaaagttccg
    40561 gtttgggtct ttacattgtg aataatattt tagaaagcta tcaaatggac tatagttttc
    40621 tcccttatga acacggtatg gaatttaaga ttagcttgta gacagattag ttttttatta
    40681 aagttcatat agggttaaca taagtgtgtt attctttgtg tagataaaag aaaggatact
    40741 aatatggtat tagcgattat tttagtaaca ttctttattc gattgatttt tttaaagcgt
    40801 tcgatagaga atgagaaacg aatccttagc aatggcggga aagaatttgg agttgagaat
    40861 acaaaacgat taactatggc tcatattgtt ttttatctct cttgcttggt tgaggcaatg
    40921 gtgcacaaga caatttttga tggcatgggc atggttggtt tagtcttgct tattttttct
    40981 atgctgatgt taatgttggt gattcacttg ttgggagata tttggacagt gaagcttatg
    41041 cttgtcaata atcacaaata tgtagatcat atcttgttta ggacagtaaa acatcctaat
    41101 tactttttaa atattcttcc tgagttgatt ggcttgacct tgttgagtca tgcttatgtg
    41161 acttttgttt tagtttttcc agtttatgca gttattttgt atcgacgaat agctgaagag
    41221 gaaaagctat tacatgaagt tataatccca aatggaagca taaagagata aatacaaaat
    41281 tcgatttata tacagttcat attgaagtga tatagtaagg ttaaagaaaa aatatagaag
    41341 gaaataaaca tgtttgcatc aaaaagcgaa agaaaagtac attattcaat tcgtaaattt
    41401 agtattggag tagctagtgt agtagttgct agcttgttct taggaggagt agttcatgca
    41461 gaaggggtta gaagtgggga tacccccaag gttacatcta gtggggatga agtcgagtcg
    41521 catctaaaaa aaatattgag tgagatccaa acacaattag atagaaaacg acatgccaaa
    41581 actgtagcgc taattaacaa gttgcaagac attaagagaa cgtattcgta taaattaaat
    41641 gttttagaag agaagccgaa agctgagttg acgtcaaaaa caaaagaaga gttaaccgca
    41701 gcttttgagc aatttaaaaa agatacatta ccaacagaac caggaaaaaa ggtagcagaa
    41761 gctgagaaga aggttgcaga agctgagaaa aaagccgagg atcaaaaaga agaagatcgt
    41821 cgtaactacc caaccaatac ttacaaaacg cttgaacttg aaattgctga gttcgatgtg
    41881 aaagttaaag aagcggagct tgaactatta aaagagaaag ctaagaaacc tttaaacgag
    41941 ggcacaatta agcaagcaaa agagaaagtt gagagtaaaa aagctgaggc tacaaggtta
    42001 gaaaacatca agacagatcg taaaaaagca gaagaagaag ctaaacgaaa agcagcagaa
    42061 gaagataaag ttaaagaaaa accagctgaa caaccacaac cagcgccggc tcctcaacca
    42121 gaaaaaccag ctccaaaacc agaaaaacca gctccagctc caaaaccaga gaatccagct
    42181 ccaaaaccag aaaaaccagc tccagctcca aaaccagaga atccagctga acaaccaaaa
    42241 gcagaaaaac cagctgatca acaagctgaa gaagactatg ctcgtagatc agaagaagaa
    42301 tataatcgct tgactcaaca gcaaccgcca aaaactgaaa aaccagcaca accatctact
    42361 ccaaaaacag gctggaaaca agaaaacggt atgtggtact tctacaatac tgatggttca
    42421 atggcgacag gatggctcca aaacaatggc tcatggtact acctcaacag caatggcgct
    42481 atggcgacag gatggctcca aaacaatggt tcatggtact atctaaacgc taatggttca
    42541 atggcaacag gatggctcca aaacaatgg
//
LOCUS       DAVBCV010000011        42534 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBCV010000011 DAVBCV010000000
VERSION     DAVBCV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.011"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(1..856)
                     /locus_tag="VQ516_000590"
                     /pseudo
     CDS             complement(1..856)
                     /locus_tag="VQ516_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1021..1215
                     /locus_tag="VQ516_000591"
     CDS             1021..1215
                     /locus_tag="VQ516_000591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511079.1"
                     /translation="MKKKRVSRMKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQ
                     LEIRVQSQKKSNGLDVGKAD"
     gene            1530..1826
                     /locus_tag="VQ516_000592"
     CDS             1530..1826
                     /locus_tag="VQ516_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2511080.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            1929..3944
                     /locus_tag="VQ516_000593"
     CDS             1929..3944
                     /locus_tag="VQ516_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2511081.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFIALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            3946..4578
                     /locus_tag="VQ516_000594"
     CDS             3946..4578
                     /locus_tag="VQ516_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511082.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            4719..4997
                     /locus_tag="VQ516_000595"
     CDS             4719..4997
                     /locus_tag="VQ516_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2511083.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(5506..5919)
                     /gene="ndk"
                     /locus_tag="VQ516_000596"
     CDS             complement(5506..5919)
                     /gene="ndk"
                     /locus_tag="VQ516_000596"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2511084.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(6030..9707)
                     /gene="rpoC"
                     /locus_tag="VQ516_000597"
     CDS             complement(6030..9707)
                     /gene="rpoC"
                     /locus_tag="VQ516_000597"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2511085.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(9740..13351)
                     /gene="rpoB"
                     /locus_tag="VQ516_000598"
     CDS             complement(9740..13351)
                     /gene="rpoB"
                     /locus_tag="VQ516_000598"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2511086.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSSLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            13767..13837
                     /locus_tag="VQ516_000599"
     tRNA            13767..13837
                     /locus_tag="VQ516_000599"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13799..13801,aa:Cys,seq:gca)
     gene            complement(14035..15366)
                     /locus_tag="VQ516_000600"
     CDS             complement(14035..15366)
                     /locus_tag="VQ516_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2511087.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(15657..16481)
                     /gene="endA"
                     /locus_tag="VQ516_000601"
     CDS             complement(15657..16481)
                     /gene="endA"
                     /locus_tag="VQ516_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2511088.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(16520..16708)
                     /locus_tag="VQ516_000602"
     CDS             complement(16520..16708)
                     /locus_tag="VQ516_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2511089.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(16701..17984)
                     /gene="murA"
                     /locus_tag="VQ516_000603"
     CDS             complement(16701..17984)
                     /gene="murA"
                     /locus_tag="VQ516_000603"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2511090.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(18008..18238)
                     /locus_tag="VQ516_000604"
     CDS             complement(18008..18238)
                     /locus_tag="VQ516_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2511091.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(18296..19333)
                     /locus_tag="VQ516_000605"
     CDS             complement(18296..19333)
                     /locus_tag="VQ516_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2511092.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(19317..19805)
                     /gene="coaD"
                     /locus_tag="VQ516_000606"
     CDS             complement(19317..19805)
                     /gene="coaD"
                     /locus_tag="VQ516_000606"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2511093.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(19795..20334)
                     /gene="rsmD"
                     /locus_tag="VQ516_000607"
     CDS             complement(19795..20334)
                     /gene="rsmD"
                     /locus_tag="VQ516_000607"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2511094.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(20399..21391)
                     /locus_tag="VQ516_000608"
     CDS             complement(20399..21391)
                     /locus_tag="VQ516_000608"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2511095.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(21503..22186)
                     /locus_tag="VQ516_000609"
     CDS             complement(21503..22186)
                     /locus_tag="VQ516_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2511096.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(22242..22982)
                     /locus_tag="VQ516_000610"
     CDS             complement(22242..22982)
                     /locus_tag="VQ516_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2511097.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            23016..23294
                     /locus_tag="VQ516_000611"
     CDS             23016..23294
                     /locus_tag="VQ516_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2511098.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            23372..24298
                     /gene="yidC"
                     /locus_tag="VQ516_000612"
     CDS             23372..24298
                     /gene="yidC"
                     /locus_tag="VQ516_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2511099.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(24373..25167)
                     /gene="pflA"
                     /locus_tag="VQ516_000613"
     CDS             complement(24373..25167)
                     /gene="pflA"
                     /locus_tag="VQ516_000613"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2511100.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(25293..26543)
                     /locus_tag="VQ516_000614"
     CDS             complement(25293..26543)
                     /locus_tag="VQ516_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2511101.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(26610..27437)
                     /gene="purR"
                     /locus_tag="VQ516_000615"
     CDS             complement(26610..27437)
                     /gene="purR"
                     /locus_tag="VQ516_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2511102.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLHEFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(27704..28645)
                     /locus_tag="VQ516_000616"
     CDS             complement(27704..28645)
                     /locus_tag="VQ516_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2511103.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(28635..29891)
                     /locus_tag="VQ516_000617"
     CDS             complement(28635..29891)
                     /locus_tag="VQ516_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2511104.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(29893..30555)
                     /locus_tag="VQ516_000618"
     CDS             complement(29893..30555)
                     /locus_tag="VQ516_000618"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2511105.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLASYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(30518..31174)
                     /locus_tag="VQ516_000619"
     CDS             complement(30518..31174)
                     /locus_tag="VQ516_000619"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2511106.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(31185..32063)
                     /gene="rsgA"
                     /locus_tag="VQ516_000620"
     CDS             complement(31185..32063)
                     /gene="rsgA"
                     /locus_tag="VQ516_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2511107.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(32656..33429)
                     /locus_tag="VQ516_000621"
     CDS             complement(32656..33429)
                     /locus_tag="VQ516_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2511108.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(34119..34991)
                     /gene="rsmA"
                     /locus_tag="VQ516_000622"
     CDS             complement(34119..34991)
                     /gene="rsmA"
                     /locus_tag="VQ516_000622"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2511109.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
     gene            complement(35242..35460)
                     /locus_tag="VQ516_000623"
     CDS             complement(35242..35460)
                     /locus_tag="VQ516_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511110.1"
                     /translation="MENMTNPLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            complement(35528..36169)
                     /locus_tag="VQ516_000624"
     CDS             complement(35528..36169)
                     /locus_tag="VQ516_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511111.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(36171..38201)
                     /locus_tag="VQ516_000625"
     CDS             complement(36171..38201)
                     /locus_tag="VQ516_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2511112.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQRLFTDYL
                     QNFSSESVEVTSQKYYLPQVSLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(38470..39369)
                     /locus_tag="VQ516_000626"
     CDS             complement(38470..39369)
                     /locus_tag="VQ516_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2511113.1"
                     /translation="MLIGQKIKEIRIEKGVSRPYFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(39441..40001)
                     /gene="rnmV"
                     /locus_tag="VQ516_000627"
     CDS             complement(39441..40001)
                     /gene="rnmV"
                     /locus_tag="VQ516_000627"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2511114.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(40001..40774)
                     /locus_tag="VQ516_000628"
     CDS             complement(40001..40774)
                     /locus_tag="VQ516_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2511115.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKVTDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(41079..41972)
                     /locus_tag="VQ516_000629"
     CDS             complement(41079..41972)
                     /locus_tag="VQ516_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2511116.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(42256..42390)
                     /gene="rpmH"
                     /locus_tag="VQ516_000630"
     CDS             complement(42256..42390)
                     /gene="rpmH"
                     /locus_tag="VQ516_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2511117.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT        12247 a         9539 c         8064 g        12684 t
ORIGIN      
        1 tatctaattc cttcaaataa ttttctatct tcatcagcat taaaggattg ttataaatct
       61 tgcatagctc tcttgcttct atataataat ttttgacttg ttctctgtct agaaatttgg
      121 ctccagcatt tcctacaaga ataagtagag gagccaattg gtagcttgtc tgtctttgtt
      181 tacagagttc gatagtctca agagcttctt ggatggcttc attatatttt tcctttgata
      241 ctaggtagtg agcgtaattg taacgaactc tgatgtagcc aaataaaaac tcttgatgct
      301 caaaattttt tgtctgatat aactccatca aatgagagta gtttacctca tattcttgtt
      361 cacgacccac taaggaatag aaattagata gagtattcaa cgcctttaaa taaatcaaag
      421 tatttgagga gacttttaat aatatatttt ccaatgaaga aattgcttca cacttactgt
      481 catattgaca gaagtcaata atagctttaa tccattcaag gtaagttcgg tcttctagtg
      541 ttagaaaagt acttcgttca atctctattc tataaatata ttccaaattt tcataatttc
      601 tatcatctaa taggcgagca gataattgct tgaaattaga gaggttagat ttaatttcaa
      661 tttgttcatt aaaaaaataa tctaatggca cttcgagtcg ttgtgagagt ttgaacaaaa
      721 ggtctgcgga gggaatgaaa taccctctct caattttact aatctggctc tattcacaaa
      781 ttccttctgc aagagtttgt tgggagagtc tcaactcttt tcttttcaat ctgaatttct
      841 ctgcgagtgt attcattaaa gataataaac cttcctattt gcttaatttc attataaaat
      901 ttttagttca aaatagcaag ttaaaggaat aaataattct ttgcggtaat aaacaaaaaa
      961 aagtcaaaaa ttacataaaa atgcttggct aaataaaata tatttgttaa aataaggata
     1021 atgaaaaaga aaagggtttc acgaatgaag aaaaatcgtg gaattcaaaa attagctata
     1081 ttagtattac taggtgtttt tatgtttagt aacacaattc cttaccaaca gtttattcag
     1141 aagaatagac aattggagat tcgagtccaa tcgcaaaaga agtccaatgg tcttgatgtt
     1201 gggaaggctg attgatttta tattacttta aaattcaact atttagagaa aggtattctg
     1261 atgagagctt gtaacaatac cagatagata taagaatgga gatgtttgat aaaatgcttt
     1321 catagctaac caactgttga aaattatgag ttacaaaatc taaagtattt aagattggtt
     1381 tataaaatta ggtctctgcc tgtagaaagg aacgtgcagt tagaaaggag gaaaaaatag
     1441 gatactataa ttcagactga gatttctaat taaaatatat agttgcattt cgctcatttt
     1501 cataatattt cattcagagg agtaataata tgttaaaatt tagaaaagta gttgcatcat
     1561 cccttgccgt tttggcattt agtttggctc ttcctgtttt ggcagtttct caccgtggtg
     1621 gcgagtggac ctatggaggc catcatgatc caaataattg gggagcattt tcgaattatt
     1681 atcatggttc acagtatcac tgggcctatg ttggaagtaa tggacgtaac aatcaaaaaa
     1741 cagtttatgc aggagcacgt agtgcggcct atgcgtttgt taatacaaat tttggggagc
     1801 aagttacatt tgatgcggga tggtaaagaa taattgaaga agattctttt atcaatagtt
     1861 cagtaagctt gttagtttat agagggcagg ggtatttctg ccctctgttt tagaataagg
     1921 aatgcactat gaaaaaatta tttatactca tatccaacct gcttgctagt ttgttctttg
     1981 tttgggtatt taccatttgg actgatacat atgtctccta ttattacccc aacgttgttg
     2041 tacatgattc ttcccctgaa acaacttttc agcatgttgc tacacgcttg gaaaaactag
     2101 cagaagaaac agatagcttt attgcgattc aacaccaaga tcctaactca gaaggtacta
     2161 cagttttttc ttatacgacc tttggggacg gaaagttgcc tgatgggctt caggaaaaaa
     2221 aactagaaga tgctcaaagt agtagtgttg aaacaaacta ttttgtattc gatggacacc
     2281 tagatattca cttgctaagg gaggagctaa gtcaacttgg cttgactaac atgaacctga
     2341 caattccatc taaactatct acgttaatgg ctatctttag taacggattt cagttaatca
     2401 gtctattgat tttcatctta acctttatag cactgacttt aattagtcaa attagccaat
     2461 tacggtcgtc gggcattcgc ctcatttcag gagagaaacg gtggtccatt ttccttagac
     2521 cagttggtga ggatttgaaa gggatagctg ttggtttcag tttagctggc gtgctcgcca
     2581 ttcttatgca aaaaattctg tcgcttccaa cgcaatccct aatgaccata ggagcaggct
     2641 tgctcagtta taatctcatc ttgttgtcga tctccctgtt tttcgctcaa ctcttcgcag
     2701 ttgggataaa aaagatacac ctgatgcaga ttataaaagg gcaagtgcct gtcagaggaa
     2761 tcattagttt gattttaatc ggtcaactat tagcgattat tattgtaacg ctgggaatag
     2821 ggagtagctt aaagtattcc caagcctggc aacagcatcg gattggacaa gaggcttgga
     2881 gtcaggaaag gcaactgatt accctatcaa tcagccgtga aggaacgagt cctggttttg
     2941 atgaacaagc tcaaaggaaa ctcagaactt ggtatcaatt gatggatctg gctgtttcag
     3001 aacaaaaggc tttcttatct agacaccagt taattgaccg tactttgcaa aatggcatgg
     3061 cttcctccaa aaacttgata acctctacag aatggcacga ttacaacccg aatggcaatg
     3121 tccttatcgt cacaccgcaa tacttggagc gtcaaaacat tcctgtagat acaactattg
     3181 agcaaaagat gaatcacctt gatgtagggg agtttgtctt attgctgcct gaacacctcc
     3241 gttcagagga agaacattat aaatctgttt ttgaagacga cttaaccagt cgcatgtcta
     3301 gtcaagatga acgacagcaa atgactgcta cggtaggtta tttagaatca ggtcaggatc
     3361 gttttgtgta taatacgacc cctatttctt accagcagtt tttgaaagat ccaatcatca
     3421 ttgttataac accccaatca actggtccac agtccatttt gttttggata gacgcagtac
     3481 agaactacgt tctctttaat caattgtctg atgcccagga gcttatccag agacaaggca
     3541 ttgaaaattg ggtctcagaa atgcaaacag gttaccacaa ctacatcaca ttattggata
     3601 atattcagag ggaacgttgg gtaatgctag caggagctgg gcttgggatt gcaacttcaa
     3661 tcttgttgtt taacactatg aataggctct actttgaaga atttagacgt gccattttta
     3721 tcaaacgcat tgcaggtctc aggttcttag aaatccatcg cacttatctc tttgctcaac
     3781 tgggtgtgtt tttactggga tttgttgcga gtgtatttct tcaggtagag ataggagttg
     3841 ctttcttagt cttgttactc tttactggtc tatctctttt acagttacat gtccaaatgc
     3901 agaaagaaaa caagatgtcc atgcttgttt tgaagggagg ttaatatgat tgaacttaaa
     3961 caggtgagta aatcttttgg agaacgagag ttattttcga atctttcaat gacatttgag
     4021 gctggaaaag tctatgcctt aattggttca agtggtagcg gaaaaacaac cttgatgaac
     4081 atgattggga aattagaacc ttatgatggg acgatttttt accgaggtaa agacttggcc
     4141 aattataaat cgagtgattt tttccgtcac gaattgggct acctcttcca gaactttggc
     4201 ttaattgaaa accaaagtat tgaagaaaac cttaagctag gtctcattgg tcaaaagttg
     4261 agtcggtcgg aacagcggtt gaggcagaag caggctttag aacaggtcgg cctgacttat
     4321 cttgacctag ataagcgcat ctttgagtta tcgggcggag aatcgcaacg ggttgccttg
     4381 gcaaaagtta tcttaaagaa tccacccttt attctggcag atgagccaac agcttcaata
     4441 gacccagcaa cttctcagtt gattatggag attctgctat ctcttcgaga tgataatagg
     4501 ctaatcatta tcgcaacaca taatccagca atttgggaga tggctgatga agtgttcacg
     4561 atggatcgtc tgaaataaaa atccttgttt ttaattgcac gatgagttac tgaaatatta
     4621 tcatgaatca ataattggag ttaatttaga attgtacttt attaatattg aggtaacttt
     4681 ttcttgataa aggaagaaat agtggagagg aagttagaat gaaaaaattc gacaattata
     4741 ttgttgagaa gccttgcgat tctaattcag ataaactgca aaaaatctta ataattgaaa
     4801 atttggtaga tgatattttg caatttcctc tcagaatcaa taatagtgta ggagagattt
     4861 tcctcctaca accgtttcaa aagaaaacta tctttattcc atgttatttt gaggaagata
     4921 ttgtgaaagt caaagatgat gataaagttg agtggaattt gttagaattt caaaaattta
     4981 gagcattttt ggcttagtaa tctgtgttga aggctcaaaa cctatggtaa aaaagtcgct
     5041 ttgaaaacgt attgcctcca aagatttagt taaataatga tttaacacaa aaagaaatta
     5101 ttgaagttct ggaaagatgt tgtttcagta ttgagaaaag gtgggaaaaa cttgcgattt
     5161 tcacagagaa aggaagaaaa agtatagaaa tatagtcaat tgaaacaaga acaggacaaa
     5221 agaacctttt gtgccatatt tttctccttt cgctttacaa ttggcttgaa cacctttatt
     5281 gtatcgcgtt tggagttttt ttggtataac cttcgacgcg cacccgcata gcgggtgttt
     5341 ttttgtctcg cacctaacgg agcgagacga actaatagtc acttaatcaa aaaacgcacc
     5401 atatcaaaaa ctaaaaagtt tgatatcatg cgtcatgtct taaactaatt gactatactt
     5461 tctattcaaa tgagctttta accaattgat tgagccaatc cactcttaaa accaaagagc
     5521 aatttctcgc ttagctgact cttctgaatc tgaaccatgt acaacatttt ggataacctc
     5581 attttctcca gcagcttttg caaaatcacc tcgaatagtg cctggtaaag cttcttctgg
     5641 acgagttgca cccatcatgg tccgccaagt ttcgattact ttgggaccag aaatgacacc
     5701 cacaagaact ggacctgaag tcatgaattc acgaatcggt gggtaaaaac tctgaccaac
     5761 caagtcctga tagtgctggt caatcaactc ttctgaaact tgtgaacgaa actccaattt
     5821 ttcgattgta aatccacgtt gttcgatgcg ctttaacact tcacccacta gccctctttt
     5881 tacaccatct ggtttgatga taaagaatgt ttgttccata cccgtctcct ttgtcagctt
     5941 ctttctttta ttttaccaca tttcgtggaa aaatggagaa agttttcaga atagagaatg
     6001 agagaacccg agggttctct cattctctat tattctactg tttcttccac agtttcaacg
     6061 gcagtatcca caactacttc tgttgtttct tcatttcctt cttcctctac tggaggatta
     6121 aggtattctt cttcgttgac agcatatggt tcaaggttac ggtaacgggc cataccagta
     6181 ccagctggga tgatcttacc gatgataaca ttttctttaa gtccaaggag atggtctttc
     6241 ttaccacgga tagctgcgtc agtaaggaca cgagttgttt cctggaagga agccgctgac
     6301 aagaaactgt ttgtttcaag tgaggctttg gtaattccca taaggactgg gcgacctgtc
     6361 gctggaactc cacctgcgat aaggacatct ttgttggcat ctgtaaagtc attgatatcc
     6421 atgagggtac ccatgagaag atctgtatca cctggatcca tgacacggac tttacggatc
     6481 atttgacgaa ccattacctc gatgtgtttg tcaccgattt ctaccccttg gctacggtaa
     6541 actttttgta cttcaccgag aaggtacgtt tcaactgaca agacatcacg aactgcaagg
     6601 agacgttttg gttggataga accttctgtc agagcagcac cacgcgctac ttggccccca
     6661 acttcgacac gcatacgagc tgtaaatgga acgacatatt caccttcgcc agtttcaccc
     6721 ttaacaaaga ctttcttggt acgagttgat gcatcttctt cgatagcagt aacttctcct
     6781 ttaacctctg tgataaccgc ttccccttta ggattgcggg cttcaaagat ttcttggaca
     6841 cgaggaagac cctgagtgat atcggtattt gaggcaaccc cacctgtgtg gaaggtacgc
     6901 attgtaagct gtgtaccagg ttccccgata gattgggcag caatggtacc aactgcttca
     6961 ccaacttcaa ccgcatcacc agtcgccaag ttgataccgt aacagtgacg gcagacaccg
     7021 tgacgagtgt tacatgtaaa tacagaacgg atagtcactt cttccacacc agcattgaca
     7081 atttcacgcg ccttgtcttc tgtaatcaat tcatttggac caataatcac tgcaccagtt
     7141 tctggatgtt taacagtttt cttagtgtaa cgaccgttga gacgctcttc gagagactcg
     7201 atcatctctt ttccttctgc gatagaacgg atcaagagac cacggtcagt tccacagtcg
     7261 tcctcacgga tgataacgtc ttgggcaacg tcgaccaaac gacgagtcaa gtaacctgag
     7321 tcggctgtct taagggccgt atcggtcata cctttacgag caccgtgagt tgagaagaac
     7381 atttccaata ccgacaaacc ttcgcggaag tttgaaagga ttggcaattc catgatacgt
     7441 ccattcggag cagccatcag accacgcata ccggcaagct gtgagaagtt tgagatgtta
     7501 ccacgggctc cagagtccat catcataacg attgggttct taggatcttg gttagcaatc
     7561 aagcgtttct caagtttttc acgggcagca cgccattcag ctgtaacagc attgtaacgc
     7621 tcgtcgtctg tgatcatacc acgacggaat tgtttggtga tttgctcgac acgtttgtgt
     7681 gattcttcaa tgatttcagc cttgtcatca acgactggga tatcggcaat acccactgtc
     7741 aatcctgcaa gagttgagtg gtggtaaccg agattcttca tgcggtcaag tagggcagaa
     7801 gtttctgtcg tacggaaacg tttgaagatt tcagcgatga tatttccgag gtttttcttc
     7861 ttgaacggag ggttgagctc aagattgctg atagcttcct tgatatctcc accaagtggc
     7921 aagaagtatt tagctggaac accttctgtc aagttggcat tgtttggttc ttgcaagtat
     7981 ggtagcccct ctggcatgat atcgttgaag agaattttac caactgttgt aagcaagacc
     8041 ttatgtcttt gctcttctgt ccaaggcttg ttgaggctgt ctgttgcgat accaacacgt
     8101 gagtggaggt gaacataacc attgcggtaa gccataaccg cttcgtcacg gtctttgaag
     8161 accattcctt caccttcgcg accagcttct tccatggtca agtagtagtt acccaaaacc
     8221 atgtcctgag atggagtaac taccggtttc ccatctttcg gattcaagat gtgctcagca
     8281 gctagcatga ggatacgagc ttctgcttgt gcttcttctg aaagtggtac gtggatggcc
     8341 atttggtccc cgtcaaagtc agcattgtag gcttcacaga caagtgggtg caagcgaaga
     8401 gccttaccat caatcaagac tggctcgaag gcttggatac ccaaacggtg aagggtcggt
     8461 gcgcggttca aaagcactgg gtgttcttta atcacttctt caaggatatc ccagatacgc
     8521 tcatctccgc gttccaccaa gcgtttagct gctttgacgt tttgcacgat atcacgggca
     8581 acgatttcac gcatgacaaa tggtttaaag agttcaatcg ccatttcacg tggcacacca
     8641 cattggtaca tcttaagagt tggaccaacg gcgataacgg aacgtcctga gaagtcaaca
     8701 cgtttaccga gcaagttttg acggaagcgt ccttgtttac ctttaagcat gtggctcaat
     8761 gatttcaatg gacggctacc tggtcctgtg attggacgac cacgacgacc attgtcaatc
     8821 aaagcgtcaa ctgcttcttg aagcatacgc ttctcatttt gaacgatgat acctggtgca
     8881 tttaactcaa gcaaacgagc caaacggttg ttacggttga taacacggcg gtaaaggtca
     8941 ttcaagtcag atgaggcaaa acggccacca tccaactgca acattggacg aagatctggt
     9001 gggataaccg gaagaatgtt aagaatcatc cattcaggtt tgtttccaga cttgtaaaag
     9061 gcatccaaaa catccaaacg acggatggct ttgacacgtt tttgtccagt agctgttttc
     9121 aattcttctt tgagttcagc aatttctttt tcaagatcta cttgcttcaa aaggtcttgg
     9181 atggcttccg cacccatctt ggcaacaaat gaaccataac catattcacg caagcgctct
     9241 cggtattcgc gctctgtcat gatagacttg tgctcaagtg gtgtatcctt aggatcaatc
     9301 accacataag ccgcaaagta gataacttcc tcgagggcac gagggctcat atcaagggtc
     9361 aagcccatac ggcttggaat ccccttgaag taccagatgt gagatacagg agctttcaat
     9421 tcgatatgtc ccatacgctc acgacgaact ttcgtacgcg ttacttcaac cccacagcgg
     9481 tcacaaacaa ttcctctgta acgaatgcgt ttgtacttac cacaagcaca ttcccagtct
     9541 tttgtaggac caaagatcac ttcatcaaag agtccttcac gttctggttt caaggtacga
     9601 taattgattg tttcaggttt tttgacttct ccataagacc atgaacggac tttacttgga
     9661 gaagctaggg tgatttgcat acttttaaaa cgatttacat caaccactat ttcttccctt
     9721 tctattctaa gtgaactgct tattcttgtt cagcagcttc ttctgttgct tccgcttttg
     9781 ttgctttctc agcttcttca gcttcaaagg ctgctttagc ctcttgagct gctttttcgc
     9841 gggctttttc aaggtcatct acgtggatga catcttcgtc cattccttca tccaagtcgc
     9901 gaagttccac ttcttggtca tcttcgtcta ggacacgcat gtcaagacca agagattgca
     9961 attctttgac aagaactcgg aaggattctg gaacacctgg ttttggaatt ggtttgcctt
    10021 ttgtaatagc ttcataggct ttcaaacgtc cgttgatatc gtccgacttg taagtcaaga
    10081 tttcttgaag gacatttgac gcaccgtagg cttcaagagc ccaaacctcc atctcaccga
    10141 aacgttgtcc accaaactga gctttacctc cgagtggttg ttgggtaaca gttgagtaag
    10201 gtccgactga acgcgcatgc aatttatcgt caaccatgtg gtggagtttg atcatgtaca
    10261 tgactccaac agaaacacgg ttatcaaatg gttcacctgt acgtccatcg taaaggattg
    10321 tcttggcatc gctatccata cctgcttctt taacagttga ccaaagatct tcagaacttg
    10381 ctccatcaaa gactggtgtc gcaatgtgaa taccaagagt acgagctgcc ataccaaggt
    10441 gaagctccat aacctgaccg atattcatac gtgatggcac cccaagtggg ttcaacatga
    10501 tgtcgactgg agttccgtct ggaaggtaag gcatgtcttc tacaggaacg atacgagaga
    10561 caaccccttt gtttccgtga cgtccggcca ttttatctcc gaccttaatc ttacgttttt
    10621 gagcgatgta aacacgaacc aacatgttaa cacctgattg caactcatct ccatttacac
    10681 gtgtaaagat cttaacatca cgaacgacac catcggcacc gtgtggtaca cgaagagaag
    10741 tatcacgcac ttcacgagac ttgtctccaa agatagcgtg caagagacgt tcttcagctg
    10801 aaagatcttt ctcaccctta ggtgttactt tacctacaag aatatcacct tctttaacct
    10861 cagcaccaat acggataatc cccatttcgt caaggtcttt gagggcatct tcaccaacgt
    10921 ttggaatttc gcgagtgatt tcttcaggcc caagctttgt atcgcgcgtt tctgattcgt
    10981 attcttcaag gtgaacagat gtgtagacat cgtccttcac caagcgttcg ctcatgataa
    11041 cggcatcctc gaagttgtaa ccttcccaag tcatgtaggc aacgattggg ttttgtccaa
    11101 gcgccatttc tccattttcc atagaaggtc cgtcagcgat gaaatcgcct ttttcaacga
    11161 catcaccaac ttttacgaga gtgcgttggt tgtaagcagt acctgagttt gaacgacgga
    11221 atttttggat gtggtaaaca tccaatgaac catcttcacg acgaacttct accttgtcag
    11281 catctgcgta agtaacttta ccatcatact gagcaatcac agccgcacca gaatcgtggg
    11341 ctgcttggta ttccatacca gtaccaacgt aaggtgcctg aggattaatc aatggcacag
    11401 cctgacgttg catattggct cccatgaggg cacggttgga gtcatcgttt tccaagaaag
    11461 gaatacatgc tgtcgcaacg gcaactacct gttttggtga aacgtccatg tagtcaacaa
    11521 tattagctgg atactcttgg ttgacccctt ggtgacgtcc catgacaatc ttctcagcaa
    11581 aggttccatc ttcattcaga ctagagttag cctgagctac agtatattca tcttcttcat
    11641 cagctgtcaa ccaaacaatt tcgttcgtga caacacctgt ttcacggtca accttacggt
    11701 atggtgtttg aacaaaacca tatttgttca agtgtccgta agatgacaag ttattgatca
    11761 aaccgatgtt aggtccttca ggtgtctcga ttggacacat acgaccatag tgagtgtagt
    11821 gcacgtcacg tacttcatat ccagcacggt cacgagtcaa accaccaggt cctaaggctg
    11881 acaaacggcg tttgtgagac aactcagaaa gcgggttgtg ttggtccatg aactgtgaca
    11941 actgtgatga accaaagaat tctttaactg cagctgttac aggacggata ttgataattt
    12001 gttgtggtgt caagacttca ttgtcctgaa cagacatacg ttcacggaca ttacgttcca
    12061 tacgagaaag tcccaaacgt acttggttgg caagcaattc accaaccgca cggatacgac
    12121 gatttccaag gtggtcgata tcatctacac ggccaagtcc ttcagccaag ttgaggaagt
    12181 agctcatctc agcaaggata tctgcaggag tcaccgtacg aaccttgtca tctgggttag
    12241 cattaccaat gatcgttacg acgcgatctg gatcagttgg agcaacaacc ttgaattttt
    12301 gaagaacaac aggctcagtc acaacggctg catcgtttgg gatgtagaca atcttgttca
    12361 agtcgccatc caaatggctt tcaatgcttt caatcacgct acgagtcata atcgtaccag
    12421 cttctaccaa gatttctcca gtttcagggt ctaccaatgg ctctgcaatg gtttggttga
    12481 gcaaacgtgt tttaacattg agttttttat tgattttgta acgaccaact gctgccaagt
    12541 catagcgacg tgggtcaaag aagcgagcca caagcaagct acgtgagctt tcagctgtct
    12601 taggctcacc tggacgaagg cgttcgtaaa tttctttcaa ggcttcgtct gtacgagagt
    12661 ccattggatt cttgtggata tctttttcaa cagtgttgcg aaccaattcg ctgtcaccaa
    12721 agatatcaaa gatttcatca tcacctgaga aaccaagagc acgaaccaag gttgtaaatg
    12781 gaatcttacg agtacggtcg atacgagtgt aggtgatatc ttttgagtcg ctttcaagtt
    12841 ccaaccaagc tccacggtta gggataacag ttgaaccata gcccacctta ccatttttgt
    12901 ctactttgtc gttaaagtaa acacctggtg agcggaccaa ctgagaaacg ataatacgtt
    12961 caccaccatt gatgatgaaa gtacccattt ctgtcatgat tgggaaatca ccaaagaaaa
    13021 cttcttgggt cttgatttcg cctgtttctt tattgatcaa gcggaaggtt acaaaaattg
    13081 gtgctgagta gctagcatcg tggatacgag cttcttctag cgtgtatttt ggttccttga
    13141 tttcatatcc aacaaattcc aactccattg tgtctgtgaa gtttgaaatt ggcaatacat
    13201 cttcaaacac ttccttaaga ccgtggtcta ggaaagcttt gaatgagtca gtttgaattt
    13261 caatcaaatt tggtaagtca agaacttctt tgattcttga aaaactacga cgggtacgat
    13321 gtttcccgta ttgaacgtca tgtcctgcca agatgattct cctttgtaaa taagttccaa
    13381 gccttgtcaa tcaggctttt ctaatcgcca tatggttgta aaccccttat caccgtgtcc
    13441 tcttgacgaa ttttcagaat ctttaagcct ctgttacaaa tgctcaaaat cttgaaaaaa
    13501 agcacaaaaa gagcagctaa atctgacttt ttcagaagat ttaactgctg tgagccttgt
    13561 ctggacaata tttcagacaa aacctacgac aaatgattac ccatattata ccctatttag
    13621 ctagattttt caaggggatt cagtaggttt ttggtaattt ttttcccata gaaaacttgg
    13681 catcacattc gaatcacgct atggtacaaa aaactgaaaa aactattgac tgaaaatcat
    13741 tttcaaggta taataataaa cgttaaggcg gtatagccaa gtggtaaggc acggctctgc
    13801 aaaagcttga tcgtcggttc aaatccgtct accgccttct ataacttgat ttatcaggtt
    13861 tcaaatgaac agaaagccca atttgaaggg ctttttttat tttccctcga ataaatacgt
    13921 ataactttaa aaacttttgg agcgagtttg tggcagagtt ctttccatgg cataattccc
    13981 ttttgaaatc aattgaaaaa agcttcgtca cgcctggtga caaaagcctc tagtttattc
    14041 tgtttcttct tctatttcga cttctgtgat ttggactttt accttggtaa cacgtccatt
    14101 cttcacttta tcattggtta agataaactg tttgttttgg ctgaccaatt catagctgag
    14161 tttctcagtc gttggaatgg ttcccactcc tgtcaaataa taaccagcga tagtatcaac
    14221 gtcatcactt tccagttcaa cattaaagta attattgaaa tcattgaggg tcatagttcc
    14281 ttgaacaatg taggtatcct cgccaatctg atgaacatct attgctgctt tgtccgtttc
    14341 atcatcaatt tcaccaacaa tttcctctaa gaggtcttcc aaagtcacca aaccagccat
    14401 accaccgtat tcatccagca agattgccat ttgtctttgg gtatttcgca gttcttttag
    14461 caagtcatcc acaaaaatag tttcaggtac aaaaagtgga tcttgtaaaa ttctcttcca
    14521 aacaatattg tcaaaaccgt ccacaaagcc tgccttaagg agactcttgg tgtgaatgat
    14581 tccaattaca ttgtccttat ccccatcata aaccgggata cgagaataat tttgttttaa
    14641 aatactttgg ataatggctt gactatcatc ctgaatatcc accataaagg catccgttcg
    14701 aggaaccata acctctcgtg ccatcagttc atcgagcgaa aagacacctt gtaacatctc
    14761 aatctcatca gcatccaatg tttcttcact atttgtcagc atataggcaa tttcatcacg
    14821 ggtcatcttt tcatccgcat catcgaatga cataggagtc aaatggctca agaaattggt
    14881 cgaagcagct aaaagccaaa caaaaggact gactagtttt ccgatcccaa tgataaccgg
    14941 cgctgtacga attgccaagg catcctttag attaagagcg attctcttag gatataattc
    15001 cccaaaaacg atggaaatat aggtcaaaaa tgccaaggat agaaaagttg ccacggcttg
    15061 tgctgtttcg ccattcccaa gccaagaggc aatcacacgt cctagagtat cagttaaact
    15121 cgcccctgat aagattgtaa tcagggtgat tcctacctga atggttgata aaaagtggtt
    15181 aggattttct agtaccttca gcaggcggat gtagcgtctg tctccttctt ccgccttttg
    15241 ttcaactcgg gcacgattaa gagaaaccat ggccatttct gtggctgaga aaaaggcatt
    15301 taataccgtc aagataaaca ataaaacaaa ttgtagcaac aaattctgac tacttgggtc
    15361 ttccatagtt cttctcctaa aatagtatct tatcctttat tatatcacaa aatttaatcc
    15421 aatacatatt atttttttct tagcgtaact tccatctctc tcccaaaact ggaagttagt
    15481 ctaagacgga tttccactga tttcacgcgt ttttctcata ctaaaagcta ggttgtggga
    15541 aactggaagt tagttttaga agttactatt ttttcttagt acctgttcta gccctgctta
    15601 aactagtaaa aagaactcct acatccataa gtgacatagg agtttaggct tatcttttac
    15661 tgagttacag ttacttctcc agttcggtaa tccagttgaa gtcccttttg aacattggga
    15721 actagaacat tgaattccaa ttctccatcc gaagacttgg cttcaatctg tgaagctgag
    15781 ggaactaaat cctcgtttga agcgtagtaa agggttacac ggtaacggac acgcttgttt
    15841 tggtccaagg ctttacgcac cttgctttca tagtagtttt gaccagtcga atactcggct
    15901 tgtgcctgat ttgcccaggc tgtctgaaca gcaatgtttt taggattgct tgttgaggca
    15961 tcaaaaccat ccaaaccacc gattaaggca tagcctaaca aatgacctct atcgactgca
    16021 tgggtataag agccctttag attcttgacc tgatgccaac ctggaggagt ccaagaagtt
    16081 gaaccattcc cagtttcttt acgattcttg tactgacgag tggccttaga caagagggca
    16141 ttagctacgg ttggaacagt ttccttaccc actgtctttg ttttattgtc agcgtagggc
    16201 ttacttgaaa ccttggcatc tagatttgtt ttattaccat tgacgataaa agcacctgag
    16261 ccattccact ccagactccc ctttatttga ctcttgactg cgtctgttaa gacactctct
    16321 gccaatgctt gactaggagc ttcaggcgct tgttttttct gactaagctt ggttttagga
    16381 ctattagctg tcgactgcat ctgcttgata taatagctcc ctgtagacaa aagcaataac
    16441 actagcagtc cgattagtgt ctgtcttgtt tttttgttca tatttctcct tatctttaga
    16501 aaaaggctgg ttctccagcc taatttcctg taaatttatg aatcaattcc tgccattttg
    16561 ctggagacag gatagcccat gaatcttgac ccttgcccaa gattccataa cctaccatta
    16621 aaccgattcc tagagccaga gtacctaaaa tcagtactat gatgactaaa agtaaacgct
    16681 tgactacgta gcttgatttc ttattcatct tcatcacttg cctcaatccg ctgaatctta
    16741 gcacctagct gcgccaactt ctcatggaaa ccgtagtaac ctctatccaa gtgaaccaat
    16801 ttaccgacca cagtttctcc ctgtgctacc aaacctgtca aaatcaaagc tgcactggca
    16861 cgaaggtcag ttgaaagaac ttctgctccc tgcaaaggct gtccaccaac aatacgagct
    16921 gtatcacgga taatctcaga atgcaagccc atgcggcgca tctcttctag gtgttggaaa
    16981 cgattttcaa aaactgtctc caccatggtt gattcgcctt ttgcaactgt catcaaggct
    17041 gtaaattgag cctgcatatc tgttggaaat cctgggtggg gcaaggtttt cacatgaaca
    17101 gcttttagat tttccagttg agaacgaaca cgaattcctt cgtcttcttc aattacttca
    17161 acacccattt caagtaactt ggcaatcaag ggacggttgt gctcccagac agcgtctcga
    17221 atcaagacat caccaccagt catggcagca gctaccataa aggttcctgc ttcgatacgg
    17281 tcctggacta cattgtgagt cgtaccatga agtttctcaa caccagtaat ggttatagtc
    17341 tctgtaccag cacctttgac cttggctccc atttcattaa ggagaatggc taagtcaaca
    17401 atctcaggct cacgcgcagc attctcaatc actgtcaccc catcagccag agtcgctgcc
    17461 atcatcaagt tctgcgttgc accaacactt ggaaagtcca tatagatatg agcaccatgc
    17521 aagcgttctg cctttgcttc gatgtaacca gctgtctgac taatcttaac ccccatagct
    17581 tccagacctt tcaaatgaag atcaatagga cggctaccaa tcgtacaacc acctggcatg
    17641 gataccttgg catgacccac acgggcaagg attggcccta atacaacgat ggaggcgcgc
    17701 atcttactga catacttgta aggggcttcc tcagtgatgt cgccagtagc atccaccttg
    17761 acaagatgag cttcctcatc aaagtcaacc ttggcattca aaccaccaac tacctgattc
    17821 ataataaaga catccgacaa aatcggaaca ttctgcaaga cggtctttcc ttcacttgct
    17881 agaatagtcg ctgccaacaa gggtaagact gcattttttg ctccctcgat cgtcacgctt
    17941 cctaccagac gattatcgcc accttgaacc acaattttat ccatactcgt ttcctttact
    18001 cttgatttta taataatcct ctaagcgctt cttgccacaa atgatacagg ctaataaaga
    18061 aagaactcag gatgtagcct atgacaatgc taaaaaaggc tactaataca cgaacttttc
    18121 ttgtattctc agctgtcact tttaaaacct tttcccatct aacaagatcc tttaaaaggt
    18181 aaaaactcac ataaataaag agcatatgac tacttagagt gaataataat tgaaccattt
    18241 gactattata ccatcttctc tgtttgtgcg ctagtttgga aacttcactc aaaaattagg
    18301 gattgttttt caagtaatca atcgcatctt gtagggtttt aacgggcacg attttcatat
    18361 ccgtcttgat tgttttagcc gcttctaggg ctgtttggta gttgtttttc gcgtccggat
    18421 gcgccttttg ttcttcttcg ctaacagggt tatcaggagc aaagaaaata gcagcacctg
    18481 ccctagccga agctacaacc ttcttatcaa tacctccaat gtctcccaca ttaccatcgc
    18541 ggtcaatggt acctgtaccg gcaacaatac gaccattacg aagatctggg tgagctattt
    18601 gagtatagat agctagacta aacatgagac cagcacttgg gccgccaata ccagctgttg
    18661 aaaagctaat tgggacattg ctgattacct ctgtacgatc aatcaagccg attccaatcc
    18721 cattttttcc attttccaag gtgataatct tcccttctgc agacttggtt tgcccatcct
    18781 cttcataggt gaccttgacg gaatccccta atttttgaga actgacgtaa tcaatcaagt
    18841 ctttggaact atcaaaggtc tgatcattga ctgctgtgac tgtatcagag atattgagaa
    18901 tccctttaaa ggttgaatta tccgtcacat tcaaaacata aactccaaag tacttgagtt
    18961 cgatatcctt accagctgtt tttagtcctt gatacttggc catattttgc gatgtttgca
    19021 tgtagaattg attgattcgc ataaattcaa catcggaaga accacctgta gtctcctgag
    19081 cactacgaat atctgtaaaa ggtgtcaacc aagcataaat catatgagct aaagtagcat
    19141 gttgaacacc aaccgtaacg aattgatagg caccagcttc cttatcttct gtgtcattta
    19201 ctttaaggac ttggcgaata tcttccgaac cacctggaac ctctatataa taaggcaagg
    19261 gcactacaaa tgccaagaaa gtcacaatca aggccgcaat gacgtataag ggccatctaa
    19321 tctttttttt catttcttat ttcctccaaa atactctcgg gaacatagca ggcaatatcc
    19381 tgaccaaact tcaaaagctc tctaacgcct gatgaactga tatagagatg ttcaggtcga
    19441 ctatgtaaat aaatagtctc tatatcagaa gacagctgat gattgtagta atcaaaactg
    19501 gcttcatatt gcaaatccga cgcattcctc aaaccacgca ctagacaagt agcacccaat
    19561 ctttttgcaa catcgaccac caattcatca tgagaagcca cgacttcaac attttccaaa
    19621 tgtcccaaag ccttttctag cccccgttta cgattttcaa gagggagaaa tccttgtttg
    19681 tggggattaa aaaaaatacc cacacaaagc ttatcaaaaa gtctgctcgc ccgttcaatg
    19741 atatccagat gcccatttgt catcggatca aatgagcctg tgaataagcc aatcttatct
    19801 gacatagact gtcaccttac taattccata aatcttttcc ttccagatac ccagacaggc
    19861 aatttcttct ggaagttcaa cggtcttatc cgtctcgcac acaaccataa catcttcaga
    19921 aaaaagctct ctctcagcca ttttttcaat atctgctacg atttgttcct tggcataggg
    19981 agggtctaag aaaacgaggt caaattcccc agatacctgt tccaatgccc tttctgcatc
    20041 cattttgagg agttgaaatt ttccaacttc cttggtcatc tggatatttt cagccacgat
    20101 ggtctgagcc ttacggtctc gctccaccaa aacagcactg gacatgccac gcgatactgc
    20161 ttcgatagat aaaccaccac tacctgcata aaggtccaag actcgtccca cttcaaagta
    20221 gggaccaatc atgttaaaaa tggctcccct aaccttatcc gaagtaggtc ttgttgtctt
    20281 gccttctagt gtcttgaggg gacgtccccc atagattcct gatacgattt tcatactgtt
    20341 tattatacca aattatagac aaaaagagaa agaaaaccga accttgcggt tcgattctct
    20401 acaaaatatt ttcgtaagta tcgcggactt cttgaggcca aacacttgtt tgcacttctc
    20461 cgatgtgtct cttgcgaagt aggaacatgg ccatacgaga ttgtccaatt cctccaccga
    20521 ttgtcaatgg gaataggcca ttcaacaaag acttgtgcca ttccaattct aagcggtctt
    20581 catcacctgt aatttccacc tgacgtctaa gagtttcttc atctacacga attcccatag
    20641 aagacaactc aaaggctcca cctaaagact cattccagac aagaatatca ccatttagac
    20701 ccttgtagcc attctcagac tcgcttgtcc agtcatcata gtctggtgca cgtccatcgt
    20761 gcggtttacc atctggcaac tcgccaccga taccaatcaa aaagacggct ccaaattctt
    20821 tacaaatcgc attttcacgt tctttaggtg tcaagtctgg gtagcgttct accaattctt
    20881 ctgtatggat aaaggtgatt tgttttggca agatagactc gatgtcatag cgggcttcaa
    20941 cagctagctc agtcaggcga atagccttat aaatcttctc aactgtttct tttagataaa
    21001 cgatgttacg cttaccattt gggataacct tctcccagtc ccactggtca acataaacag
    21061 agtgggttgc atccaaggaa tcctcatctg gacgaagggc tttcatgtgg acaaagagac
    21121 cctctccttc accaaagcca aaacgagcca aggtgtggcg tttccattta gcaagtgagt
    21181 gcaccacttc ataagtagca tcagggattt ggagaacctt gaccgatact ggattttcca
    21241 caccagacag gttgtcctgc attccgtcac cgaccttact caagatagga ccttgaactt
    21301 cgacaacttc tagcttatct ttcaaatact gggtaaaagt gtttttgaca aaggaaattt
    21361 cttcttgttg atggataaaa cttttcttca tagacaactc ctcaaaaatt taataaacgt
    21421 attatacacc tatttccaag aaaagaaaag agaaaatttt aaaaaaatca gtgtcactca
    21481 aaacactgat ttcatagacc ttttagtcat tgcgaccaaa gatacgtaga atacttagga
    21541 aaagattgat aaaatcaaga tagatgctga gagccattga caccacccag cctgtcgcta
    21601 cacgaccttg tgattgttca taggcgaggc gaatcttttg gttatcccaa gcaatcaacc
    21661 ctgaaaaaac caaaaccatg gctacgctaa tcatataatc aaagaagcca ctagccaaga
    21721 aaatattgac taccatagca atcagtaaac caatgagagc tgccatcatt gctcgaccaa
    21781 tcccactcaa gtctttcttg gtaaaaatac caactgccgc catgacaaag aagagaaggg
    21841 cgctcgatac aaaggcagat aaaactgtac ccggagtata gaaagccacc acaaaactaa
    21901 gggtaaagcc gtttaaaacg gagtaaagta aaaatactgg aagagcagct ggactatttc
    21961 ttgaagccat gctactggca acgaaaacca aagccaactc cgcaaaagta gcaatggtca
    22021 accagagacg cccctgcatc aacaagtaaa ccaactgaga ctgaaagacc gtcaacataa
    22081 ggcctgatac caaagcagat agtccgattc ccagaccaac aaaggcataa accttagcgt
    22141 aaaattgatt gagacctgca cggtcatgta taatagtgtg attcatcctt ttctcctttt
    22201 ctgtgaacat ctttccttga ttataacaaa aaaagttaaa attagcttaa atggaagatt
    22261 aaaataccgg ctgcaactgc aacattcaaa ctctcggcct gccccttcat gctaatatgg
    22321 accaactggt ctgcactttc agccataagg ggactaattc cttgaccctc atttcccata
    22381 actagtacaa aattttctat agaaggcagt tctctgtaat caacagaatc tttagatagg
    22441 gttgttgcta gcactgggat agctaccttt ttagtttcgt caagaagcgc ttgactagtc
    22501 atccggtaaa tgggcagatg gaaatgacta ccttgcatgg aacgtagggt tttgagactg
    22561 tagatgtctg ccgacttatc tgaaacaatc actcctgtaa aacctgctgc atccgcagtt
    22621 cgaatgatag ttcctacatt accaggatct tgcacatctt ccaaaaacaa gaacttgccc
    22681 tgactcaaat cagcttgtcc tacttcttct ttttgaacca cggcaacaat tccctgtgga
    22741 gtctgagaat ccgccaaatc tagcaaaata tcctctgaaa tccagacagt ttgcggaaaa
    22801 gcagctaact gatttcgata attttctagg gcaaagattt tctcaatcgt cactccagct
    22861 tggacagctt cttcaaacaa gtgccagcct tcaatcaaat aggcagactt gcggtatttt
    22921 ttttggtgta atttcttggc attttttacc acagaattgg cttttgaggt tataatagtc
    22981 atagaaatat tataacacaa tcaaaggggt ttggtatgca aaaggttaga atgattgccc
    23041 aaggtagggt gcagggagtc ggctttcgtt ggggtgttta tagcctggcc cttgaaatcg
    23101 gtggcatcac aggtcgggta tggaataacg acgatggcac agtggaaatc ttagcccaag
    23161 cagactcatc tgctatcatg gcaaaattta tccaagaaat ccgaaaagga ccgacacctt
    23221 tttcaaaagt aagctactta gatgtcaaac taagcaactt tcctccctac tctgacttta
    23281 aaatcgcaaa ttaggtctcc agaactattg tatattttgt aaaaaaacag tagaatagaa
    23341 aggtataatt tttaaagaag gaataaaaac agtgaaatct attaaacgtt ttgcattctc
    23401 ggctatggga gtggctatgt tgcttgtctt gactggctgt gtcaatgtcg ataaaaccac
    23461 aggtcagcca acaggattta tttggaatac gatcggagcg cctatggctg aagccatcaa
    23521 gtacttcgct actgataaag gtctaggctt tggtgtcgct atcattatcg taaccattat
    23581 cgtacgcttg attatcttac cacttggtat ctaccaatca tggaaggcaa cgcttcactc
    23641 tgaaaagatg aacgctctca agcatgtcct tgagccacac caaacacgtc tcaaagaagc
    23701 aactactcaa gaagaaaaac tcgaagccca acaagccctc tttgccgctc aaaaagagca
    23761 cggcatcagc atgtttggtg gtgtaggatg tttccctatc ctccttcaaa tgcctttctt
    23821 ctctgctatc tactttgctg cccaacatac tgaaggggtt gctcaagcaa gctacctagg
    23881 cattcctcta ggttctccaa gtatgatttt ggttgcctgt gctggtgtcc tttactatct
    23941 tcaatcgctc ctttcacttc acggagtaga agatgaaatg caaagagaac aaatcaagaa
    24001 aatgatttac atgagcccac tcatgatcgt cgtcttctcc ctcttctcac cagctagtgt
    24061 cacactttac tgggttgtcg gtggtttcat gatgattctc caacagttta tcgtcaacta
    24121 tatcgttcgt ccaaaacttc gcaaaaaagt ccgtgaagaa ctagccaaga acccaccaaa
    24181 agcaagtgct ttctctaaac caagtggacg aaaagacgtt acccctgaac aaccaactgc
    24241 tatcacaagc aagaaaaaac acaaaaatcg caacgctgga aaacaacgtt cgagataaga
    24301 agaaaaaggc ttagctgatt tgctaagtct ttttgcaaat caaaagcccg atgtttccat
    24361 caggcttctt ttctatccat gtacacgttt catataatct tgataacttt cggtatccat
    24421 gagtttttta gcgttcttga cgcgatctgc tgttggtggt ttgactcctt cgagggaata
    24481 tggaattcca agttcacgcc acttgaactc acccatggtg tgataaggta gaatttcaaa
    24541 cttatcaaca tttttgaggg tcttgacgaa cttaccaagt tcaatcaagt catcatctct
    24601 gtctgtcaat cctggaacta gcacgtggcg aatccagaca ggttttccaa tatctgatag
    24661 atactgggca caagccaaga tatttttatt agtttggcta gtgacaatct tgtgctgttc
    24721 ttcgttgatt tccttgatat ccaaaagaac caagtcagtg acagccatga gtttgtcaaa
    24781 cttctcaagg taacgtggtt tattacggaa aggaagagca caggtatcca aggtacagtg
    24841 gattccttgt tccttagcct tggtgaagag agcaatcagg aaatcaatct gcaagagagc
    24901 ttctcctcca ctgactgtaa tcccaccctt atttccccag aaaccacggt agcgcaaggc
    24961 ctctgtcaag acatcatcta ccgtccgttc acgtgactta ttggactcca tagcccaagt
    25021 gtctgggttg tggcaatact ggcaacgcat gtgacagccc tgcaaaaaga caataaagcg
    25081 aataccaggc ccatctactg acccaaagct ttctgtcgaa tgcaccattc ctgtcacttg
    25141 tccataatca attgtttctt cagacatatc gttaccttcc ttgaaaacgt tttatagttt
    25201 tattatatca cgacttgaag gaaaaacaag atttttgcct attttttaga aagcaatctg
    25261 tttttcaatt tcattttcaa tctattatca gattattctt caaaatcgaa gccatataag
    25321 gttgcaaaat agtcctcagg gcgctctgca cggcggattt gacgggcttt accttcttcg
    25381 gtatagagga tttccgcaga acgtaatttg gcattatact ggtagcccat tgaaaatccg
    25441 tgggcacctg tatcatgaat taccagcaaa tcaccgattt ctgtatgagg cagttcgcga
    25501 ttgactgcaa atttatcatt gttttcacag agtgaaccaa ccacatctac cgcttcagct
    25561 ggtccatctg gatgggtcac gttgctaata tgatggtaag ctccgtacat agctggacgc
    25621 atgaggttga ctgctgaggc atccacacct agataggtac ggtaggtttc cttcttatga
    25681 gtcactcttg tgactagagc accgtgaggt gccagcataa aacgacccaa ttcggtgaaa
    25741 atcttgacct gaccaagacc tgctgacgta agaacttctt catacacctt acgaactccc
    25801 tcaccaatca aggcgatatc gttcggctcc tggtctggac gataattaac accaataccg
    25861 ccagaaagat tgataaagtc tagcgaaatg cccaactttt ccttgatttc aacagccagt
    25921 tcaaagagct gacgagccaa ctctggataa tagagatggg tcacggtatt ggacgctagg
    25981 aaggagtgaa tcccaaaagt cttggctcct ttttccttca agatagcaaa ggcttcaaag
    26041 agctggtcct tggtcatgcc aaacttagcc tccccaggat tgtccataat gtctgtcccc
    26101 agttcaaaaa cgcctccagg attataacga caagagatga tttctggaat gcctgctact
    26161 ctctccagat gttcaatatc ttcaaaggca tccaagttaa tggtcgcacc caattcacgt
    26221 gcataggcgt attccttgtc tggcgtgttg ttggaagaga acataatctc agaacccaga
    26281 aagtccagtt tatggctcat caaaagctct acataactag agcagtccac accacaacct
    26341 tcttcttgga gaattttcaa aatagctgga gttggagtag ccttaactgc aaaatattcc
    26401 ttaaagccct tgttccacga aaaagcttgg ttgacggctc ttgccttctc acgaatcccc
    26461 ttctcatcat acaagtgaaa gggagtcggg aactcggcaa caatcgcttc taactcttct
    26521 ctattgataa atggtgtttt cataagcttc cttctattct gtttgcaaag aaaggctggg
    26581 acaatcgttc caacctttag ttctatctct tatttatctt cgtcaaagat attgccaacc
    26641 tcaacatcga tggcttggtt cttgacatca atattggtta ccttcaagag tgacttgtag
    26701 tcaaactgct tttcacgttc ttcttgggca ttgtccgcaa agaccgctac acctgccagt
    26761 tctgagtcga actcgtgcaa gagactaatc ataccattga ccgttccgcc acctttcaag
    26821 aagtcatcca caatcaagac acggctgcct gccttaagac tacgttttga aaggaacatt
    26881 ttctcgatac ggtcaccact tgaacctgaa acatagttga cgctaacagt tgaaccttcg
    26941 gtaattttca ggtcacggcg cacaatgaca aaagagacat tgaggacatt ggcaactgca
    27001 tttgcaagtg gcacaccctt agttgctacg gtcataaccg cgtcaatttt ttggtccata
    27061 aagcttttgg caataatacg accaatattt ttcaagatgg ctggtgtgct aagcaaatca
    27121 gacagataga tataaccacc tggcaagata cggtcacttt ctgacaactt ggtacgcaag
    27181 tcttcaacca tttccttagc atcctgactc gaaatagacg gtgtgaagat gacacctccg
    27241 ccagccccag tcactgtctg gatatgaccg atttcaattt cctcaaaggc gcgtttgata
    27301 atgacgatat cttctgagat ggatgattta gcagactcat acttttcagc aaaagtattg
    27361 agactagtta gtttataagg attattaatc aaatagttgg aaatgacaac catccgatca
    27421 cttcttctta atttcatttc tatttcctat tccattttta ttcaaaaaat caaaaagcaa
    27481 actagaaagc tggtcgctgg tggttcaaaa cactgttttg agattgtcaa tagaactgac
    27541 aaaccctgta atatacctgc atatatacat acgacaaggc gatactaccc tagtttgaag
    27601 agattttcga agagtattca tttttgtctt ttacttatta taccatattc acataaaaaa
    27661 acgaacattc ttatcctaaa aaatgctcat ttttcttaaa ttatcaatct aaatctggtt
    27721 tatagaagga acgattatcc atagcgaaga ttttattggt catctctcct ttatccacca
    27781 aagccagagc tgttgacatc atcatcatgc ttgcatccag attgtcaatc atatggataa
    27841 tctctgcctc cataatacgt ggacggactg gacttccata ttcaagcaag ccgtggtgac
    27901 tgaggatgac atgacgaagc aaaacgactt cttccttggt atcatcgatg ccgagttcca
    27961 taactgtctt ggtaatttcg ctatcaatga gagcgatatg tccaagaaga ttacctcgca
    28021 ctgtgtactc tgtctggtct ggccccgtca actcgataac cttagctaag tcatgcagca
    28081 taatccccgc atagagcagg ctcttattga gctgaggata aacttcgcta atagcgtctg
    28141 ccaaacgtac catggtcgcc gtatgataag ccaaccccgt ttcaaaggca tggtggttgg
    28201 tcttggcagc tggataggag tagaattcct tatcatactt ggtgtagaga tttcggacaa
    28261 tccgttgcca gacaggattt tcaattttga aaatcatttg cgacatgtag tcacgaattt
    28321 ccttgacatc aactggtgac ttgaccttga aatcggctgg gtcattgggt tcaccagctt
    28381 gaggcaggcg gagagtaatt tgattgactt gaggggtatt gttataaact tctcggcgtc
    28441 ctttcatgtg gacaacctta cctgcggtaa aggcctcaat gttatgaggt tgggcatccc
    28501 agagcttccc atcaatctcg ccactatcat cttggaaggt aaaggctagg tagtttttcc
    28561 cagctcgagt ttgcctcagg tcagctgatt tgattaggta aaagccttca aatagctcat
    28621 cttttttcat gtgactaatc ttcatattct tcctcatttt cttgaaaatg gagtagatca
    28681 agcgcaggct caccttctga caactcaatg tgacggagcg tccgctcgat agctatggta
    28741 cgacggttta ataattcatc aatattgcca gaggcatgtt ggagatgttt ttgtgccttg
    28801 accagaatac caccaaactt gccaaactcg gtcttgacac tggcaagagt cttgctgata
    28861 tggtcggcac tcttttggat attaagggtc ttgaaaccaa ctgatagaga gttaagaagg
    28921 gctgataggg tacttggtcc tgcaacaata atctgttctt cccgtctcaa atcatcaaag
    28981 aagaccggat tgcgcacaat ctctgaataa agaccttctg tcggaacaaa caagattcca
    29041 aagttggttg tccgaggtgg tgctatgtac ttgttcctaa tatccctagc aaagcgcttg
    29101 acgcttgcta ggagtgactt acgacagcgt tcaatctcat ccttgtcacc tgtctcatag
    29161 gcttcttcca agcggtaata atctgccagt ggaaacttag agtcaattgg cagatagacg
    29221 tattcttggt cgccttgtcc gggtaacttg atggcatact ccactcgttc actagagttt
    29281 tcaaccgttg cgtattctcg ttcgtactgg gcaggtgtca tgatgtcttc aataatttgc
    29341 cccagttgca attctcccag aatccctcgc gtcttggttc cagagagaac cttgttaaga
    29401 gctccgacat cacgggcaac tgtctgcatt tctccaaggc cacgattgac agactccagt
    29461 tgtttagaaa ctgtctcaaa ggaagcctgt aagcgtgtct gcaaggtctt ttctagtttt
    29521 tcctcgaccg tctggcgcat ttgttccaaa cgttgctcat ttgattcctg caaggcttgg
    29581 agacgttggt ctgtcttgtc tctagtttgg aggagattat ctgtcatttc ttgacggact
    29641 tgggtcagac cttggtgcaa ttcaatccgc acttcttgca aacggtcgct gacaaccact
    29701 tccaaatctt tttggtctaa ctggctggct tgtctggctt ggtcaaagcg gtaatccaac
    29761 tggtctgaca aatgatctgc ctgatcctcc aagctcttac ttaagtgttt ctcctgccta
    29821 tcctgccttt gccaaatcag aaagagacca gctagattgg caattaataa tagaataagt
    29881 aaactttcca tcctacctcc tgtccttgct atgcagtacg accacataac catctgggca
    29941 agttaccgac acttccctat ctatatattc gttagaagcg tacacttttt taaagaaaaa
    30001 attttcctct gtcaactcat acttggctcc aagaatagtc agctggctat cccgaactgg
    30061 cataaaggct agatagtcgt agtctgaacg aggttctagc tgactgattc cttctggaca
    30121 ataagtaatc aagttttgcc catcctcaat ttctatttga tgcatatagg atgccaactt
    30181 aggattgcta ggcagaaaga cattggccaa catatggtca atacggccac ccaaggcacc
    30241 gaaaatagta acctgagcct gaggattttg cttaaagatg gtcaagagag ccaattccaa
    30301 atcagtatca tctttttctg gccgggcttg gacaaaatac tgggcacgtt tttgaatcac
    30361 ctgtcgctct tcttccgtca cagaatcaaa atctccgact gctagagcaa gaggcaaatc
    30421 ttcttccaag atccagagcg agcctcgatc cacaccaaca aaaccatcaa agtccgtccg
    30481 ataatgaccg cggtctccgc ctgcaaaaac ggcaacccta gtccagttgt tttctgagag
    30541 tttgtactcg ctcattgaca tctcccttaa agacatagga acctgctaca aaaacggtcg
    30601 caccagcttc tttggcttga gcaatagttt ggtcatcaat cccaccatcc acttcaatct
    30661 caaagttcaa acctttttca tcacgaaggg caaccagctc acgaacctta tccatggttt
    30721 ctggcagaaa ggcttgacca ccaaatccag ggttaactgt catgactagg acttggtcaa
    30781 ctagatgaag cacatgctta atcgcttcaa ctggtgtacc aggattgata acaactgatg
    30841 gcttgacacc gagtgaacga atcttttgga gggcaccatg aatatgaggc gttgcttcca
    30901 catggatact aataatatcc gcacctgcac gcacaaagtc ctctaaatga tgctcagggt
    30961 ttgacaccat caagtggcag tcgaaaacca tcttactatg aggacgaaga gcttcgacca
    31021 cacccgcacc aaaactgatt tgcggcacaa aatgaccatc cataatatcg atatgggcat
    31081 actctgcccc agttgcttct aggcgtttaa tttcacgttc aaagttggca taatctgctg
    31141 ccagaattga cggagcaatc ttgtattgag acataggttt ctccttattt tggaattttt
    31201 ttgctgactt ttttataggt ttctctgcga ttttcaattt cgctgaggaa ttgcaggtag
    31261 ttgtcaaaac ggaagggcgc aatggtacct tcttcaactg ctggcttgac agcacaggac
    31321 ggctcatggg tatgggtaca agtacggaat ttgcagtctc gactgacagt ggcaatctct
    31381 ggaaaggcct gattgaggtc ttcagccgtt gatacttcat aatccagtga tgaaaagcct
    31441 ggtgtgtctg cgattttacc cccattgagg ttgtaaaaac taacagctcg agtggtatga
    31501 cgcccgcgac ccagactgtc tgaaatttct cccgtttcaa gattaagatc tggtgcgatt
    31561 ttattgagaa gagttgattt cccaacaccc gtctgcccca taaagaccgt aaccttgcct
    31621 gttaacaaag gcaggagttc ctctttactg gtcacaaagt catatccgat gtcaccatag
    31681 gtctgctggt aaaaatccag ttctcccccg tcttccaaca aatccatttt agaaatatag
    31741 acgatgggat gaataccctt atgctccaaa agaaccaaga aacgatccag caaattgctg
    31801 ttaaaatcag gttccttgac agacatgatt actacagctt ggtcgatatt gacaataggc
    31861 ggacggacca gactgttttt ccgttcgtga atcttgagaa tatagccttc tgaattttcc
    31921 tcggcagaga aatctaccca gtccccaacg taaggggtat gccctttttt acggaaattc
    31981 ccacgcgcgc gtgtttgata gacttggcca tcactctcta catagtagaa cccagccaag
    32041 gctttaatga tttgtccctg caaaataact cctttaaaat tgttagttcc attataacaa
    32101 aaaaaagcta ttttagctta gcttctcttt acaatttcga taagtctatt ctaaaagaaa
    32161 aagatacact cacgctttag ataccaagtt ttatacaaaa ataacgaatc tacctcctta
    32221 tataaaaaca ggcgaacaga gagcctaaaa ctccctgttc gctttacttt ttctgtgtgt
    32281 aatagaaaaa acctaataaa gcaagacaaa aaatatgaat ataaactcca tacatcatgt
    32341 ggggaaagac agactctata aaatagcctg gtagatagat gattggaaat tgatagaaat
    32401 cataggtcgt tagagatagt aaacaaagaa catacataaa tactgctaca attagaaccc
    32461 atccttgctt tcgccataga aaaatcatca ataagaacag acaaagtaga tagattgacc
    32521 aatttcgcaa cataaattga aaaccataaa tcatactttc agtcgtttct ccatcaatca
    32581 catcaactac tttatagaaa ggtaaacaat agctcactaa aaaaagaagg ggactaatta
    32641 tttttttcat attctctacc atggattatt ttgcaagtaa gctgaccatc tatctccata
    32701 tcgaatcaaa ttaacagtat aattagttac tacccgattt tcacctggtc caaccttaga
    32761 aaaacctgtg ctatactccc aactacttct tgcacctgga tgaaaccatt tttgctggga
    32821 tgaataccaa ccaccttgtc taacttccac acttgaagat tctatttcga tatccctttc
    32881 atggaagtca actcctccac ttattttagt aatatttttt tgtacacctc catcataact
    32941 cacccagtga acaacagtat acaattccac actataagta ctatcaaact gactttgatg
    33001 agtagtaatg ttttgagtat aagcgtgcac tttctgtata ccaaaaatat ggtctaatat
    33061 attcgttggt ttagcaagct tattgtaacc ctcttcaaca aaagtagcct tctctaaatc
    33121 agcggtataa acagtttttc cttgtactac ttgattgttt atagaagtaa tacggctatc
    33181 cacaacaatg ggggccacca tatctccatc gctagtttct attttagctg cctctttcac
    33241 ctcatataca actcgatttt cttcagactg aatcacttct accccatttt tttcaggtat
    33301 agataatcta gtttgcccat tagaaaccgt tatatttgga ccaatattct tctcatttgc
    33361 ataagtcgca ataggaaatc ctgatagaac cactgcacac ataaggctat tgactaattt
    33421 ttttctcatg aatatatcct tcccattttc aagaggagta aaaatagcta atataagatg
    33481 aacgtttcct tatatttcag aataggatat ctatctgaaa gcaattcaca ctcctataga
    33541 taactgttcc aaatcttggc tacatatatt tcaatagttt gctacaaaaa actcctttct
    33601 aagtctattg aacatgccct tattcaccta ctaatatatc acaaaaataa ataaatacaa
    33661 gactgttttt tctactagtt ttatgataga atagtattgt tagggtaaat ttcatatata
    33721 aatgtcgagt gaaaaatcga tggagcagaa atcggtagac aaagactcga tttcgttggt
    33781 ccagccccac aaggctaatc aggtatttca cgagtttaaa aatctaatga agtgcccaat
    33841 caactactga ttcattgatt taatacgaac tatagtggat tgaacctaga aatagtacac
    33901 taaggattct aaaacgtttc tagaaattaa tttgactttc ctaatctctt tgttcatatc
    33961 ttatttcaat ctactatata taccaaaatg ctaggacaaa agtctttaaa atcagagaaa
    34021 cgcatattat caggtgttgg aaaaccttga taatatgcgt tttatcatag aaagatctac
    34081 ttcatttttt actaaaatta agtttttgtc cctgcatctt agagtccttg ccctttaagt
    34141 gcatctgcta ggctggcaaa ctcttccaag ctgagagctt ccccacgcac acttggtgac
    34201 aagcctgctt ggtccaaagc cttggtcagc ttgtccttga cctcttcagt cttgccaaag
    34261 taacctgtca agttattcca caaggtcttg cggcgatggg taaaactagc cttggaaacc
    34321 ttaaagaaaa agttctcgtc ttccactgct acagctggca ctggacggcg caccattttc
    34381 aagatggctg agtctacatt tggtgctggc acaaagactg tacgcggcac gataaaggca
    34441 accttggctg tcatgtaata ctgcacagcg atagacaagc taccgtaagc cttggtattt
    34501 ggttgagccg agatacggtc cgccacttct ttttgcatca tgaccacaaa ctcactaaaa
    34561 ggaatgccac tctcgattaa gtgcatgaga ataggcgttg tgatgtagta aggcaaatta
    34621 gccactacct tgattggcag gtcaggattt ttgaaattct ggatatgttg cgccaaatcg
    34681 accttgagaa tgtcctcgtt gactacagtc acattgtcaa aatcacgcaa ggtatctgcc
    34741 aaaatcggta ccaaacgatg gtcgatctca aaggccataa cttctgctgc acgctcagcc
    34801 aaaaactcgg tcaaggcacc aatacctggc ccgatttcaa tgacattgac ctggtcatca
    34861 atctcagccg tatccacgat tttttgtagg atattggtat ccgtcaagaa attttgccca
    34921 aaggactttt taaaggtaaa accgtgacgc tccagcactg ccttggtcac gctataatct
    34981 gcaattctca tctattctct tttctattat ctgttactac tattgtaaca cattcccctt
    35041 gtattttggg ggtgaaaatc ataccaaaaa actatctttc ctcatgaaga aagatagtct
    35101 tggaaatctt taaaagtggc cttagcgctt ctctcgaaac atacgttcat actcttcgaa
    35161 aatctcttca aaccacgtca gcttcacctt gccgtactca agtacagcct gcggctagct
    35221 tcctagtttg ctctttgatt ttcattgagt atcaaaaaaa tcagcttctt gataacttcg
    35281 attttcgggt agggtcaaat ccacatcctc ctccacatca tccactgtca gttcaaccgt
    35341 ttgaggtaaa tttttcaaac gataatgctt ttgcctttgc ccttcactac tagcaaaatc
    35401 aagcaaatag ccatgcactc ggatttgaac caaaaggaga ggattggtca tattttccat
    35461 gatgaggata tcttataaac cacctctggc actaggataa tcagataccc tgcgaatacg
    35521 gattttttca cacatgagca agtttcctca tatcaatgat ttcatcagcc ttattccaga
    35581 ctaggggatt atgggtcgca atgatgataa ttcgattttc atctttcaaa tccaccaaga
    35641 gattcataac ctcctctgaa ttttcaggat caagagctgc tgttggttca tctgccaaaa
    35701 tcaagggtgg atttttcaaa atagttttcg caagggcaac tcgttgggcc tctcccccag
    35761 ataaagtata aattttttgt tctaaatcca aataccctag attaactttt tctaaagccc
    35821 ccacttgcct ttccaaacgt tctacttttg agattttctg accaacaaaa cccaaatcca
    35881 aattttcttt gattgattgg ttttctaaga ggccaaaatt ttgaaagaga tagcccatct
    35941 gatctcgaaa atactcacga gtgggaatgg tttttaaatc tttcccctga tagagaaccc
    36001 ttcctccatc tatcttttct agctttccca agatattcag cagcgtcgtc tttccgcttc
    36061 cactctttcc gattaaggca taaaccttgc ccttctccag cttgagattc aaaccagaga
    36121 aaatcgcgcg gtcattaaat ttcttttcca atccttgaat atcaatcatc ttattttcct
    36181 ttcaatactg ccatgctacc agcttcttct tttttgtcct gccttactaa aatcaagagg
    36241 gcgttaagcg taaacaaagc tacaactaga gcgctaatca aaccatctct tgtcaaaata
    36301 ctagatagga ctaggccaag caagagaact cctccttgcg ccagtaaata cttgccatga
    36361 agctcataga ttgtcatacc agcaagacgt ttaatcataa gctcccgtct gaactgctca
    36421 aaatagagaa gattcatgga atcaaataac aagatagccg tagacagggt caaaatcgtc
    36481 ccaataagga gagagtaaaa ctctacccgt ttgtcattta gtaccttggc aaaaaatagc
    36541 tggcttttta ccaagtaaga aaccttgtga tacagacctt gctcttttag agctgtgatg
    36601 ctgtctccat atcgatctag tttcaaggca ctattagccg tagttcccca caacatccct
    36661 gcaacaggtc ttgttccagt cgcttgcggc gttagaacta caataatcgg atcttttagg
    36721 tactggcgtg ttgtcttgta cccatcatta tagaggaaac gttcctgtcc tgtttctgta
    36781 aaagctagac ttacctgtgg gaggtagtat ttctgactcg tcacttctac actttcagat
    36841 gaaaagtttt gcaggtaatc tgtaaacaat ctttggtagt aggtagactg ctctcgcaag
    36901 ctctcaggca agattagccc aaactctccc tcagacaagt tttgcatctt gtccataaac
    36961 tctgaagaaa cggtaatctt ttcttctatc agatagcgtg gtgagacata gataacattc
    37021 cctgacggtg tgtagtcact gagacgattg ccatctaggt ccacttctgc tccgtctgag
    37081 aaattgtcaa cattgctcat aatataaaaa gagtctgtat tggctaaccg ttcttcagta
    37141 aatgtctgcc actcacgatt atccttacgc gttccttctt catcggcaaa tgcactagac
    37201 caaccaaagg atagacggta acggtctgag gactggctcc atttattgct agctctctcc
    37261 atttcctgca tttcacggta gtggggtagg agagctgtcg cactcgatcc gaccaccaat
    37321 acagctaaga gttgccccac catcatcaat gtcatcatac gtttgagagg gagtttccct
    37381 ttcaatagat ccaccagact attttcctgt aaaccaagta gatagacgac acttagtaag
    37441 gtagaaatcc ctgccagggt caatccataa agtagaagag caatgatgac cagttgcacc
    37501 gttgccataa acaaggcacc ttgataccag agaatcccca atcccaaaag actggatacc
    37561 agcactgagc agataaactg tctcacatct tctaacactg gtctgagagc aactccaaac
    37621 aagctctcac cagctattaa gcgaatccct gcctgacgaa gggatttgat ccgataaatc
    37681 agggtcagac tcataaaggt cagcagaaaa atagctaaac tcagtagcac cataggggtg
    37741 gccgccaata gtaagactat cgaaaatgga tcttctccat tcgaaacaaa gccttgatag
    37801 ccaagttctt tcaaggtggt ctgtaagctc actccatcca aacttcctga cacaatcaag
    37861 taagacccta ataaatcact cgtttctgca ctctccttgg aggaaatggt cagaccttct
    37921 ggaagctctc cctcaccata agtggcatag gtaaagagtg tttctccagc ttcatttggc
    37981 tcaacgattc gacgggcgat gagactatta tgctctaccg ccaagtgact caaaacctct
    38041 gtcacctctt cgcgactagc tactggttga tttatcatca ttcctgtcac atcaacagaa
    38101 ggatagttac caaataaaat ctcttctcga tggtcaacgg aagttatcac catataaagc
    38161 gataccaata ccattgaaat caaaataaat aaacgtttca tcatttcctc caaaaagtta
    38221 cttggtcact tagtgagaag gtgaccacag tcactgcgat gttaagaagc ttattaagtt
    38281 ctttctacta gcaacatctt caaatacatt atatccgttt tttcaagaac tatcaaggac
    38341 atcaatgtcc ttaaaatcta actatttcat attattttta gcttatatct aaaaaaacag
    38401 ttctaaagac gaactttaga actgttttga tacgaggaca aggacatttt tgtcactgat
    38461 tcactatgtt tataaatatt ttttgacatc ctcttgccat tcctctgtca atttctttac
    38521 tagaaatcca tctccaatta gcttcgcaaa tgttttagaa gactgataaa atgattcagc
    38581 taaactaaaa tctctttctt ctcttaatgc ttgcttccac aaaaacataa atacaagagg
    38641 tttcttttga taatcttgag ttttatccat aatttcttgt aaacacgata gataatcatt
    38701 aaaacaatca gttactccta ccttttcaag acaacacatt cctgaaataa gagtatctct
    38761 aattataaag aagttttcta atttagttac ctttttctgt tccaaaagaa agagcattag
    38821 cttttcgatt tgatttataa atttggcaag atcttttcta atgagcattt gataaaaaaa
    38881 caaacgaatc aacaacatat catttgttga ataaacttct ttttctatga tctgagacat
    38941 atgttcctca agtattaact ctgcatattc aggccttcta gttctagaag tatccaaaca
    39001 cgcctgcaat acttcacatg ctaattgttc ttctttagga agattatcaa agtatgtttt
    39061 ataaatctct tccaaacacg cttccttctt atcgtactcc tcttctttac catagattgg
    39121 ttcacgtaaa atctggtatt tcaattctaa ataagcagga ggaagagctg aaaaatccgg
    39181 cataaggcta taaactggaa ctcctagccg gcgagcaata taggctaact tgggcaaact
    39241 cggttgcgaa gctccacttt caattcgcga cagttgacga actgtcagtt cttgctcatc
    39301 tccacaaaaa tatggacgac taactccttt ttctatccga atctctttaa ttttttgacc
    39361 aattaacatc agacgcctcc tcttctacct gcttcgaaaa atactgtgaa acttaaaaaa
    39421 ttgtaacggc tacatctttc ctactcataa gatttcatag cttcttccac ttctgccaaa
    39481 gtaaccccaa acaactctag gcgtttgagg agttgcttgc cgttagaata gccgattcgg
    39541 agagtctctc cgagatattc tctacgctta cggctgtctg cccctgctag aaaaccaagg
    39601 cgaatcaagt cgctacgact aatgtcaaac tgactctcat gttcaaattg ttctgtcacc
    39661 tgagctagag ccgttttcag gtcttcatag ctggcatgct caattcccag agaacgcccc
    39721 ttggtcttgg actttggaac agcttcatct cgcttgagaa aggcatgccg aactgttgga
    39781 atggccgtca taatcatgcg ccgaatccgc tccccattaa aatctgggtc tgtaaagaca
    39841 atgactccat gacgttggtg caggcgctga atccgctcta tatcctgagc attgatggca
    39901 gaacctcgag tctcataggt ctccacatcg aaataacgtt tgagattgac cgtatcatca
    39961 cgcccttcaa ccacgataac ttgagaaatt ttctctttca ttacttgctg tccaatccaa
    40021 aaatgcgttc tgcatttgca gtcgttgcta ccgccagctc ttctgtcgtc ataccacgca
    40081 agtcagcgat aaagtcgacc acatagcgag tataggctgt tttattttca cgaccacgct
    40141 tgggtacagg tgctaagtaa ggcgcatctg tttccaccaa aatcttgtcc aaaggtaact
    40201 ctttagctgc ttcttggagg tcagttacct tcttaaaagt caccactcct gagaaggaaa
    40261 tggtcatacc aagatccaca aacttctctg cccactcaag cgtccctgaa aatgaatgca
    40321 tgataccacc acgaggacca acgccctcac tcttgataat ctcataggta tcttccagcg
    40381 catcacgggt atggacaaca aaaggcaaat ccaagtcctt agatagctga atctgacggc
    40441 gaaaaacctg ctcctgcacc tctttgggcg ctgtcatcca atggtaatct aagccaattt
    40501 cacctaaagc cacaaccttg ggatgtttta acttatccaa caagtaagcc tcaacttcct
    40561 ctgtataagt accagcttct gtaggatgcc aaccaatagt cgcatagagc tgatcatact
    40621 catctaccaa ctccaaggca cgctcaatcg tcggtttatc aaaaccaaca atattcatct
    40681 gtgtcacacc catctcagca gccaaggcaa tttcttctgc ctcacgacct gcaaattctt
    40741 ctacattcaa gtgtgtatgt gtatcaaaaa tcatctcttc taacctcgtt ttctatcttc
    40801 tattatacca aaaaagagga ggggcaccta atttttcggt ttcccctcct ctcttcaata
    40861 gagagctatt ctgctatctt ttctatccga tattgcccat ctcctattcc acagttagag
    40921 acagaagaga ttggctacat tgatagaata ttcctattca gctaaagtat gtgattatct
    40981 tgggttctca cttttctcac ccatcactag gattgatgac ttctcaaaat cccccttata
    41041 atctaacttt aagtagagaa tagaattttc tagctgttct attttctacg tctagtcttt
    41101 cttcctagca aagcaaaacc acttgttgcc accactagac caagactagc aagtgctgca
    41161 cttgatgctg aacctgtgtt aggtaattgt ttgccagcag gtgactggta ggtaacatct
    41221 tgcattgttc ctgcagttgc ttgcggtgca cttgttgtct taggagcagc attaggagcc
    41281 actggtctct taggaactgt aaccatgaca atggcttgca ctccaatcgt ttctgcttcc
    41341 ttcacatctt ccatagtttt cgcattttca atctctttga gagctgcttg tttttctgct
    41401 tccactcttg ctaaaagttc tgctttttct ttatcagaaa gcggtgcgcc tttgatttca
    41461 tcctgcttgt ccttggctgc tttttcaatg gcatttttag ctgattcttt ttcagtagct
    41521 aattgcttag aagcttgtga ttgagctgag ttttgtgaac ctgtgtgagc tgatccaccc
    41581 gcctggctac caccgcctgc attcgcatta gcatctgagc ttccagtatt gcctccctta
    41641 tctccacctg gtgtattttc tagcttatac tctggtgtag cttcttgagt tacagattca
    41701 cctttcgcag attgttgaac tgtatctttt cctagaatcg tcactactga accatctggg
    41761 aatgtgattg ttgctgtacc atctccagct acattgattg tcgctccgtc taatgctgaa
    41821 ccatttgctt gtaaaatagc aaccttaact ttttcttcat ctgttaaatg agttatatct
    41881 tttacatcta ctctttgagc aggtgcttta acagttgatt tttcagcagt cggtttagtg
    41941 ctatcttgtg cttttggatc ttgtgctacc aattgatttc ctggaattgt aatagctgaa
    42001 ccatctttaa agacgattgt cgctgcgcca tctccagcta cttcaacttt atagtaaaat
    42061 gaaataataa caggacaaat cgatcaggac agtcaaatcg atttctaaca atgttttaga
    42121 aatagaggtg tactattcta gtttcaatct actataacat cgcttccgaa tccatttgca
    42181 tctactgaac ttttttacaa aaataaaaat cagtagactc gagttcctac tgatttttga
    42241 tagaattcgt ttggattatg cagccaaaac tttgcgtcct ttacgacgac gagctgccaa
    42301 tacgcgacga ccatttttag ttgacatacg gttacggaat ccgtgtttgc gcgcacgacg
    42361 aagtttactt ggttgataag tacgtttcac gatgaatacc tcctcataga ttttgtattc
    42421 gtttagccgg ctataaagtg atcagttact aaacatactt tactattcta tctgattctt
    42481 tcacttttgt caatagctct aggcaaatgt ttccagcttt ttcgaatgca agcc
//
LOCUS       DAVBCV010000012        37452 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBCV010000012 DAVBCV010000000
VERSION     DAVBCV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.012"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..777)
                     /locus_tag="VQ516_000631"
     CDS             complement(<1..777)
                     /locus_tag="VQ516_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2511118.1"
                     /translation="MSKETVVYTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVY"
     gene            complement(873..2183)
                     /locus_tag="VQ516_000632"
     CDS             complement(873..2183)
                     /locus_tag="VQ516_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2511119.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(2310..3305)
                     /locus_tag="VQ516_000633"
     CDS             complement(2310..3305)
                     /locus_tag="VQ516_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2511120.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFGGDKTTKKI"
     gene            complement(3667..5148)
                     /locus_tag="VQ516_000634"
     CDS             complement(3667..5148)
                     /locus_tag="VQ516_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2511121.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            5591..5755
                     /locus_tag="VQ516_000635"
     CDS             5591..5755
                     /locus_tag="VQ516_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2511122.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            5839..6405
                     /gene="amaP"
                     /locus_tag="VQ516_000636"
     CDS             5839..6405
                     /gene="amaP"
                     /locus_tag="VQ516_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2511123.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            6417..6587
                     /locus_tag="VQ516_000637"
     CDS             6417..6587
                     /locus_tag="VQ516_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2511124.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            6626..7234
                     /locus_tag="VQ516_000638"
     CDS             6626..7234
                     /locus_tag="VQ516_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2511125.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            7265..7468
                     /locus_tag="VQ516_000639"
     CDS             7265..7468
                     /locus_tag="VQ516_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2511126.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(7578..7856)
                     /locus_tag="VQ516_000640"
                     /pseudo
     CDS             complement(7578..7856)
                     /locus_tag="VQ516_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8054..8341
                     /locus_tag="VQ516_000641"
     CDS             8054..8341
                     /locus_tag="VQ516_000641"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2511127.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(8619..9389)
                     /locus_tag="VQ516_000642"
     CDS             complement(8619..9389)
                     /locus_tag="VQ516_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2511128.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(9404..10252)
                     /locus_tag="VQ516_000643"
     CDS             complement(9404..10252)
                     /locus_tag="VQ516_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET2511129.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDRFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(10249..11724)
                     /locus_tag="VQ516_000644"
     CDS             complement(10249..11724)
                     /locus_tag="VQ516_000644"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET2511130.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTSLKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(12047..13402)
                     /locus_tag="VQ516_000645"
     CDS             complement(12047..13402)
                     /locus_tag="VQ516_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2511131.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(13412..13729)
                     /locus_tag="VQ516_000646"
     CDS             complement(13412..13729)
                     /locus_tag="VQ516_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2511132.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(13746..14063)
                     /locus_tag="VQ516_000647"
     CDS             complement(13746..14063)
                     /locus_tag="VQ516_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2511133.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(14076..15761)
                     /locus_tag="VQ516_000648"
     CDS             complement(14076..15761)
                     /locus_tag="VQ516_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET2511134.1"
                     /translation="MKNKKLISSLFILFLLIFIHVKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(15766..16662)
                     /locus_tag="VQ516_000649"
     CDS             complement(15766..16662)
                     /locus_tag="VQ516_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2511135.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(16665..17927)
                     /locus_tag="VQ516_000650"
     CDS             complement(16665..17927)
                     /locus_tag="VQ516_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2511136.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQGALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPSWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            18207..19385
                     /locus_tag="VQ516_000651"
     CDS             18207..19385
                     /locus_tag="VQ516_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2511137.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(19613..20059)
                     /locus_tag="VQ516_000652"
     CDS             complement(19613..20059)
                     /locus_tag="VQ516_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511138.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLPLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSGLKLE"
     gene            20129..20788
                     /locus_tag="VQ516_000653"
     CDS             20129..20788
                     /locus_tag="VQ516_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2511139.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCVIIFGKLLL
                     V"
     gene            complement(20840..21991)
                     /locus_tag="VQ516_000654"
     CDS             complement(20840..21991)
                     /locus_tag="VQ516_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2511140.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(22006..22737)
                     /locus_tag="VQ516_000655"
     CDS             complement(22006..22737)
                     /locus_tag="VQ516_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511141.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            22966..23067
                     /locus_tag="VQ516_000656"
                     /pseudo
     CDS             22966..23067
                     /locus_tag="VQ516_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(23039..23815)
                     /locus_tag="VQ516_000657"
     CDS             complement(23039..23815)
                     /locus_tag="VQ516_000657"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2511142.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(23808..25031)
                     /gene="trpB"
                     /locus_tag="VQ516_000658"
     CDS             complement(23808..25031)
                     /gene="trpB"
                     /locus_tag="VQ516_000658"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2511143.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(25009..25608)
                     /locus_tag="VQ516_000659"
     CDS             complement(25009..25608)
                     /locus_tag="VQ516_000659"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2511144.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(25595..26362)
                     /gene="trpC"
                     /locus_tag="VQ516_000660"
     CDS             complement(25595..26362)
                     /gene="trpC"
                     /locus_tag="VQ516_000660"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET2511145.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNTGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(26359..27363)
                     /gene="trpD"
                     /locus_tag="VQ516_000661"
     CDS             complement(26359..27363)
                     /gene="trpD"
                     /locus_tag="VQ516_000661"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2511146.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(27374..27940)
                     /locus_tag="VQ516_000662"
     CDS             complement(27374..27940)
                     /locus_tag="VQ516_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2511147.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(27937..29298)
                     /gene="trpE"
                     /locus_tag="VQ516_000663"
     CDS             complement(27937..29298)
                     /gene="trpE"
                     /locus_tag="VQ516_000663"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2511148.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(29799..29876)
                     /locus_tag="VQ516_000664"
                     /pseudo
     CDS             complement(29799..29876)
                     /locus_tag="VQ516_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(30481..31482)
                     /locus_tag="VQ516_000665"
     CDS             complement(30481..31482)
                     /locus_tag="VQ516_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2511149.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            complement(31590..31958)
                     /locus_tag="VQ516_000666"
     CDS             complement(31590..31958)
                     /locus_tag="VQ516_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511150.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            complement(32054..32764)
                     /locus_tag="VQ516_000667"
     CDS             complement(32054..32764)
                     /locus_tag="VQ516_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2511151.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            complement(32767..34458)
                     /locus_tag="VQ516_000668"
     CDS             complement(32767..34458)
                     /locus_tag="VQ516_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2511152.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            complement(34470..35480)
                     /locus_tag="VQ516_000669"
     CDS             complement(34470..35480)
                     /locus_tag="VQ516_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511153.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            complement(35583..36650)
                     /locus_tag="VQ516_000670"
     CDS             complement(35583..36650)
                     /locus_tag="VQ516_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2511154.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            complement(36675..37334)
                     /locus_tag="VQ516_000671"
     CDS             complement(36675..37334)
                     /locus_tag="VQ516_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2511155.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
BASE COUNT        12046 a         7633 c         5730 g        12043 t
ORIGIN      
        1 gtacaccata gtaattgttg agtttaatgg gccaccttgc gtcataacca ttggttgtac
       61 aatcagctta aaagcagaaa ttaaagttgt taacaataca aacagggctg ttggttttag
      121 taggggcatc gtgatataac gaaactgagc ccattttgag aatccatcta attcagcagc
      181 ttcatagaca tcttgtggaa tattctgcat tcctcctaaa aataaaagca tctgataacc
      241 tgcaccctgc caagcagaaa caaaaacaat tgcatacata gcttgtttag gactagtcaa
      301 gaaaggttga gaagcaatac ctaccttatt taaaatagca tttaataagc cactatttgg
      361 atttaaaaga taaagccaaa gaatagaaat aactaccaag gacatgacaa ctggagcaaa
      421 aaaagccact ttaaaaaaca tatttccttt tcgcttttta ttgacaatca aagccattcc
      481 taaggccgct cccaattgaa ctggaataat ccaaacaaca aatttcaaag tattcaagaa
      541 gctctttaag aaaatcggat cttttgttaa tcgaataaaa ttttcaagtc caacaaattt
      601 tctttcatct ggtgtcaaca aataataatc tgtaaaagcg taataaatga ccattccaac
      661 tggaattact aaaaagatac ctaataaaat taaagctgga gctaaaaaac tataacctaa
      721 tatgttatcc tttatttttt cacgtctttt tgtatacaca acagtttctt tactcatatc
      781 catctccttt aatcatatat atcaaactag gctattaaca agaaagggga aggtagttcc
      841 aaacattttc cttccacctt cttttttaca gattatttga gtgactgatc aatagcagtt
      901 tgcatttctt ttgtacgagt atccaagact ttttgcacat ttggattttc ttcataatag
      961 ctaatatctt gcattgcttg ttgaaaagct cgagaaacct gaggatatgc tacgacaact
     1021 gggcgagcat gagctgtttt tgaattttgt tccatcaaga aacgcattgg ttcggatact
     1081 ttatctttga tattttcaat tgtagattta cgaattggaa ggaccgaatt ccccaagcta
     1141 ataatttcac ttgattctgt atttgtagca aatttaacaa attcagatgc ggcctctttt
     1201 ttatcagatt ttgtcgtcac agctaattgc caactacctg aaggggatac taattttttc
     1261 gtcttactag atactgggta aggaaggata ccaaaatcaa tatctttgta gtttgtattc
     1321 atgtcagcaa tcgtccaaga accactcaaa agcattggat actctcctgt ttcaaaacct
     1381 ttttcgactg gactaacagt tgtatagcct tcttttacaa gattttgaat gaattgaact
     1441 gcttctacac tctctttgga gttgaagtat ccttctgcct ttgttccatc ttcgtttacg
     1501 acagaaccat tatttgacca aatcaacggc atataagcat atggcaacat ttcatcgttt
     1561 gaattaatac gaaaatcaat tgctggtttg ttataatgat ctttcaattt tttagcaatc
     1621 gtattaaatt cgtcccatgt ccatggcttt tctagcgttg gaagctctga ttccgcaatt
     1681 ccagcctctt tgaacatttt tttattgtaa taaacaccaa cattcgattc agaaaatcca
     1741 aatgcataga atttcccatt ataagttccc tgttgtttaa tactgtccaa aacatcatcc
     1801 atattattat cttttagata atcatctagc ggagtaatta ctttagattt tgcataagct
     1861 gccgtatttg gtccatcaag ggtaataaca tctggaagac tattagtcgt aatagcagca
     1921 ttaaccttgt cttcatatcc tccaccacta ccactacgag gaatatactc agttacaacc
     1981 ttgtacttac tttttcctga ttgattaaag ttatcaacaa ctttttgcca agcttttcct
     2041 tcatttgttt cgtcagagaa ttgtacccag actttaatat catttgaaga actcgcttgt
     2101 tttgagttag ttcctccact acaacctgca agtatagcta acgatagccc cgcaacaaca
     2161 gataacattg tacctttacg catcattttt ctcctttttg aaacgtttca ttttttattt
     2221 attttttaaa caagcttcta gcctattttt gaaacgtttc atttgttgat tatattataa
     2281 cagcgctttc attaattgtc aagcgctttt taaatttttt ttgttgtttt atctccccca
     2341 aaatatgtgg gaattacgat tcgtttttct agaatctctt ctcgcttaat cattttcagt
     2401 atcattgcaa cagattcttt tgccatcata ccaatatctt gaactattgt agacactaga
     2461 taagattgat cgacagaagt ttttaaacca tcaaacccaa ttatctggta atcatcaggt
     2521 gctattttcc ccagttcttt cataacacta ataactctca gagccatcat atcatttatt
     2581 gtaaatatac catcaatttc tttatgagat tgcaaaaaaa ttcttatctg atcttcagga
     2641 tttattaaag gttctttcat ttcaaaatta tagacatcta ctcctaattc cttagctttt
     2701 tctctgaatc ctttacttct gagtatagtc tcattctcat acatattaat tgaaccaata
     2761 aaagcaaggt gcttattatc ccttctaacc agttcctctg ctgcagtttg tcctcctttg
     2821 taatttgctg aagtaacata gcatacattt tccgaaaaat gtctatctat acttacaaac
     2881 ggtaaatcag ataaaatata tttgtcaata tcagaatatg taataccaat aattccatcc
     2941 actttatttt gtttcaacat ttcgatatat tcgatttcat tttgtgaatc gctatcagcg
     3001 ttacaaataa ataatttata attatatttt aataactctt tctcaacatg ataggcaaac
     3061 tccgaaaaaa atggatgcca tatgctcgga ataatcaaag cgatagtttc tgttctattc
     3121 tttttcatac ctcgagcata attatcaggg atatagtcca atgtttttat agccctttga
     3181 actttcctta aagttacttc tttaatgcct ttttctttat taattacacg tgaaacagtt
     3241 ccaacactaa ctcctgcttc taaagcaaca tctttcatgg taattgattt tctttgttct
     3301 accatattat cacctccttt caatatatag tatcatgcaa atgcttttta agcaactatt
     3361 tctcaatcat ttttggccag atcatttatc ccatcatgaa taaaatcact ccaattagct
     3421 tttgaaaata cttcaatttt catgtgtaaa catctacata aaacaggaaa agccttggtt
     3481 ttatggcttt tttcgtatct tctacaaaaa aagcaagagt tttagagggc tataaatcta
     3541 gatgtacatt ttgcttaaat gattgaaggt cttttcttaa caaaaacacc ccaaaaatta
     3601 gattttttct gtctaacttt tgaggtacag ttcaaacgcg aaatagcgtt tttttgttat
     3661 ttttggttac tcatctaatc gaataaacat catggcattt aacaaatata tgagtgagac
     3721 cgtgtttata ttatttgaat agatgagtct cttattttca ataggaggaa taataaaatt
     3781 agaaataatg atatcataag atgaatcttc taaagattcc tttgataatt ctaattcagt
     3841 ccaaacttcc agttcaaaat tattgctaca gtaataagaa agtgtctctg caacgaattt
     3901 tgcatgatac tgatcaaaat tactcataac caaaaccttt agtttaggct gattttgtag
     3961 caaattaatc accaaatgtt tggtatgagt gatgaaggta taagatagat gatttaccat
     4021 cattgaacta gaacaaacct caagagtctc taaatagtga gaaagctctt tttttatatc
     4081 tgaaacaaat tttggaaaaa tattttgaaa gttcctgatt gtattccctt tttgatcaaa
     4141 taaaataaac tcagtaaaca actcttgacg atacagatgt gcggtattat gcagatgcca
     4201 aatcagatta tccttattct ccatttcaat ctgatacttg actgaaatct gatcaataaa
     4261 atcactcaat agatggtaag atttttcaac ataactatcc ttttttacgc atttcataaa
     4321 gagactttca tctatgaaaa acattttttg aaagtaagac acaaataatt ggcaaacaac
     4381 ttcttcatct aaagagatat tgtattctga ttcaaaactc tgagcaacac cttctattcc
     4441 ttctgcctgc attaaaaaat ccaaactttg gtcgttaaaa gaatctttat ctacttccat
     4501 aaaatgacca aactttattc tatataggtt cgtaactagg agcaacttta gcattctatg
     4561 cgttgacaaa ttcattggaa agcttgtttc cttataaacc aattctaaca attgagatag
     4621 tggctctgat gaaaaatttt caaatggcca ttctaggaaa taatattttt ctgaaaaata
     4681 ttgtgcaaaa aagtaacgaa tgtctctctc atttccaatg atttgaacag gggtcagact
     4741 gacttcaaat tgaaattgcc ttttaatcac tttattgatt tggctaataa tacgatagag
     4801 cgaagatgaa ctgatataaa attctttaca aatactctca gcttgacaac cttcattaaa
     4861 gaagatgaat tctaaaatcg aaaaatgagt tgaatgttta aagaaatgat ggtaaaccat
     4921 ttcaatatca ctatcatcgg tattaataat gcgtatacca ttagtagaag aatgaaaaat
     4981 caagtcagga aaagcagatt taacatggga tagatcatct ttgactgcac gttctgtaca
     5041 atttaataac tctgctagtt cagaacgatg aaaccaacgt ttatgttcaa ataataattc
     5101 taataattct aattgcctat gacttttttt agataataaa tctctcatga atatctttct
     5161 ctctttataa attatcggat taaacctctt gcaattatac cacaaagaat aggtatagca
     5221 tgatataacg acttttccta aaatctttta tttcgtataa taacactacg gagacaatat
     5281 ataaacaatt ttcttatttt accgtctatt gagggcgtga atacagaatc aaattcaagt
     5341 ctaaagatta tatttttaat tttaaaaatt atataatagc aacaattaaa gaatttgatt
     5401 ttttaaaatt atataataat aacaatcgaa ataattgact tttctatatt aaagttatat
     5461 aatagtatta atcaaagaaa ttgatttttt gatattaaaa taaaaaagga gggtaggcag
     5521 tgttgtgatc aattattgct ggaggtctta ttggtctctt ggcaggtaaa atcactaaaa
     5581 aagtagttct atgggaatca tcgcaaatgt attcgctggt ttagtcgggg catatgcagg
     5641 acaatctctt ttaggtagtt ggggtccatc aatcgctgga atggctttgc tcccatctat
     5701 tgtaggtgca gcgattgtga ttactgtagt gtcattcttt acaggtagaa agtaaacttt
     5761 tcgccagtaa agttagcaaa ctatttttaa atcaatgacg ggaaaaatag tttaaatgtt
     5821 aaatcgaaag gattgtatat gtcaaaagca aagaaaatat gtttcattat tttctgtatt
     5881 ttaatcttga caattttcct tcctgttttg atagattatc atcaagttag tgatctaggt
     5941 attcatctac ttagctggag acagaactcc gtagttgaat tctatcttgc tagatatgtc
     6001 ttttggggga cagtggttct atcaacttta gttttattat ccattttagt tgtgatgttt
     6061 tatcctaaac gttacttgga aatccaactt gaaactaaaa acgatacatt aaaattaaag
     6121 aattcggcaa tcgaaggttt tgttagaagt ttggtgagtg atcatagatt gatcaagaac
     6181 ccaactgttc atgtaaattt acgaaaaaat aaatgtttcg ttcatgtaga aggtaaaatt
     6241 cttccttcag acaacatcgc tgacagatgc caaataattc aaaatgaaat aactaatgga
     6301 ttgaagcagt tttttggtat tgagcgtcaa gtaaaacttg aagttgcagt aaaaaattac
     6361 caaccaaaac ctcaaaacaa aaagactgtt agtcgtgtga agtaaggaag taaaaaatgg
     6421 aatggcttaa acaatatcga tatccaatta tcgctggtct cataggcgta tttctggctt
     6481 gtttgattgt ctcctttggc ttcttcaaaa caatatttgt attgatttta ggagcactgg
     6541 gagttgcagc tggattatat atcgaaaaaa actatataga taaataaaaa aataaaaatt
     6601 actaatttaa ttaaaggagt ttcatatgtc aaacgaaaaa aacacaaata ctaacgtaga
     6661 aaagaaagat gctactgttg tagctcacga aatcaaaggg gaacttactt acgaagataa
     6721 agttatccaa aaaatcattg gtctttcact agaaaacgtt tcaggtcttt tgggaatcga
     6781 tggtggtttc ttctcaaatc ttaaagaaaa aatcgttaac agcgatgacg taacaagtgg
     6841 tgttaacgta gaagttggta aaacacaagt tgcagttgac ttaaacgtta ttgttgagta
     6901 ccaaaaaaat gttccagctt tatattcaga aatcagagaa atcgtatctt cagaagttgc
     6961 taaaatgact gacttggaaa ttgttgaaat caacgtaaac gttgtcgaca tcaaaactaa
     7021 agaacagcat gaagcagact cagtaagcct tcaagatcgc gtatctgacg ttgctgaatc
     7081 aacaggagaa ttcacttcag aacaattcga aaaagctaaa tctggtcttg gatctggttt
     7141 ctcaactgtt caagaaaaag ttagcgaagg tgtagaagct gttaaaggtg cagcaaatgg
     7201 tgtagtatct cacgaaaaca ctcgtgtaaa ctaagataaa ataaatataa caggagaaat
     7261 tatcatgtca gtagaagaaa aattaaatca agctaaaggt tctattaaag aaggtgttgg
     7321 gaaagccatc ggtgatgaaa aaatggaaaa agaaggagca gctgaaaaag ttgtttctaa
     7381 agtaaaagaa gttgccgaag acgctaaaga cgctgtagaa ggtgctgtag aaggtgttaa
     7441 aaacatgttg agtggcgatg ataaataagg ttaaaagtta ctttatcttt ttggtaacat
     7501 tagtcaaaag agtctgagtc aagatgattc tcagaaaaca aaaagctaga gattcccaat
     7561 tgcggaactc tagcttttta attttgcctc tttctcttat tatatttcag caggttgttg
     7621 gccatgagta tgaatcccat gtcaattctc acttgacgct tacctctcag atgacatctc
     7681 ttataaccca aacaaacctt tatctgccca aagacagatt tcatatcaat cttacgttta
     7741 gcgaaaattt gtctaccctt ggaagataaa agtgcctgat attctttagt ttttaaacac
     7801 tggtaacgtt cattcatata cagtcccttt tgaggggctg attcaggttc ataatcgcag
     7861 tcaacattga tttcaaggct gtttgctttc tatctcccct ttttcatgat atattttcgt
     7921 acaggttctt caatagtttc tatagtaaaa tgaaccaaaa atagtacacg ttgcggtata
     7981 atgttcttgt ggcatattca ttagattttc gtaaacagtt ctctcctatt tgtgagcgaa
     8041 caagtatagt caattgattg tcagtttgaa gaccaagagt cgtaaggcaa aggatatggc
     8101 agaatccatc caagggtggc tggcacaatt tctagtgaac ctctttaaat ctattacgtt
     8161 tgactgtgga aaagagtttt caaaatggaa agatatttcc aatcatcacg atagtgagag
     8221 cttttttgcg aatctaggat gttcaagaca gcggcgtctc aacgaacatt caaatagact
     8281 tttaaggtgt catgacttgc caaagcagac agatttcaac gaagtcagtc aagaatttta
     8341 ggctggacaa aaactcaatt tcaggagaaa atgaaataaa tattcccaca ataaaacgca
     8401 tagtatcaaa gtttttccaa cacctgatat tatgcatttt ttaattttaa agactttttc
     8461 ccagcctcaa gaaatcacat cccacctaaa tcctttggat accaaatacc tcatcaggta
     8521 ttttctgagt tggtaagaat gtctagctta atttgacaaa tggagggcgt taaatccagt
     8581 ttttacagga agtctattta aatattttca cactgacctt acaaaatatg aaataaatta
     8641 tataaagtca ttcccatgac aaagaataac atccctttaa aacagtatct tacccaattt
     8701 tcaggtaaga cttttgatac aaccgtcccc aatacccctc cgcatataga agcaagaaaa
     8761 ataactggaa caggtgctaa atgatactga tttaaacctg taaccacaac aattgtagcc
     8821 aaatgagaca gctgggaaaa gaaaataatt gcaagagaat acatcgttgc ttcttttatc
     8881 gaaatagaaa aaaacaccat caataaagat acattgagcg gaccaccacc aatccctaaa
     8941 aaactagaaa ttaagcccaa tagaagacca caaatcatat agaatcctat cttatcaaat
     9001 tgataagttt ttttaaaatc tgttaaaaca aatgatacaa gtagcatcac aaatagtaaa
     9061 atcatttgaa caatggatac tagccgtact gataactgag ataacaaaac ttgaaaaata
     9121 agatgaccta gaaatcctcc taaaacagaa gaaaaggata ccgtaaaaat caatctccat
     9181 tttatctctt tactttgtga aaatcttttg attgttgaaa taatcgccat tataaaaaca
     9241 gagaaactag aataaaaagc aatatcactg accgaatggt atccaaaact atccataagt
     9301 ggcttaatta taattccacc acccatgcct gatatagaac ccaatccact agctattata
     9361 atgattagaa aataaataat ctcaaccact ttttaagtcc atcctattct ttcaaacatc
     9421 taaaacctag atggctacta gtggaagtag agatacatcc atttctagca gctactcggt
     9481 atcgattaca ataagaacaa tgacataaaa aggaacctcc tctgatagca tactcctcat
     9541 cttgtatccc atattttggc aactcatagt catcttcatt aaaactagct aataaggtat
     9601 atctcggatt gcgacaccat tcccaaacat tcccaatcat ttgatataga ccaaaatcat
     9661 taggtaaaaa ttcatagaca ggagccgttc ctataaatct atctaaggca gtattttcat
     9721 aaggaaatct tccttgccaa gtattggcat gataatatcc accttctaaa agagtatctc
     9781 cccaaggata ttcagattgt cttcctcctc tagcagcata ttcccactgt gcttcagtag
     9841 gtaaagacat tccgctccaa ttacagaaag ccaatgcatc ttctaaagca acatgaacta
     9901 ctggatggtc ttctaaaccc actagattac tgttctcacc gtagggatgt ttccagcaag
     9961 cgttcggaac ttgtaaccac cacggagccc cagcaggatg aggatatccc tccctttctg
    10021 cctctggaac gaacaatata aaaacaaaag accactcttg tcgctcagct agtgttttat
    10081 agcctgtttc tttcacaaat tgagcaaatt cttgattcgt aactggagta tccgcaatgg
    10141 aaaaatcctt tacactaaca gcgacttgag gaccttcaaa atcatctaaa aagccctctt
    10201 cagaatttgt tcccatcata aatgtcccac ccttgatttt tttcattttc atgattgact
    10261 atcccccgtt ttcgttagag ttggcgttat tttagaaagt tcaacaggaa ctaactcttc
    10321 atccacaaca aaaccttctt ctctatatct aagaaagtct actaattttt tcttcatctg
    10381 acgaacaata ggttggtatt tttcgcttgg atacaaatca ttcatttcat gcggatcttt
    10441 tttcatatca aataactgat aatgatttaa aacaggaaac cagataaatt tccactggtc
    10501 tgtcaagata tattgactag aatcttttcc taaagcatgt tcaccatgaa attctgttcg
    10561 ccacccctcg tactgtccaa ataataaatt cttaacactc cgtccatcta gttcatctgt
    10621 agtagttcct cctgccaaat ccacgagtga aggaaaaata tcttgaattt tcactaattg
    10681 cttgattgtt cccctattac cagctatcaa tccagccgga tcgtaaataa acgaaggaat
    10741 gtggatactt ccctgataag gatatccctt tctaaacaga taatgttcac caagctgatc
    10801 tccatgatcc gaaacaaacc agataatcgt atctttatca tgacgaaatt cttttaggga
    10861 agtcaaaaag cgactaattt gatggtctat atgagtaatc aatccaaaat aagcagcaac
    10921 cattcttctt tgatcatctt cctttaattt ccctcttaaa gcataaatag acggaatttg
    10981 cttttctaat acttcccaat tcccgatatg caaatcgaga aattgaggca agcgttccat
    11041 atacatatca aaatagtatt ttgggggatt taatggagca tgtggttttt caaaagacat
    11101 tttgagaaaa aaaggaaccg ttggatctct tctttgtaaa aatgaaatgg actcactcac
    11161 aacccagttt gttggatgta acttctcatc tttatcccaa ggtctagctt cccaagaatt
    11221 acaatccatt ccgtcatcaa tcaaatcaac atcataacca actttaccct ttaaaaaggc
    11281 tagatagtca ctaacataat caaattgact tccataagcc ttatcatact ttctatcaac
    11341 atgtagatac ccatcgtgaa gtaagacatg atcaaaccca agtctctgtc tggaaggaaa
    11401 aacatgcatc ttaccaatac actctgtttg ataacccata tctttaaaga cttttggaag
    11461 agtattagta aaattccaag gcacctcatc ttgataacca acacgtccac ttttatcttg
    11521 gtctaagcca gtcaataaag ctgccctagc aggaacgcaa gaaggaactg gagaataggc
    11581 attttcaaaa ttataaccta cactagccat catatctaat gttggagtgc taactagctt
    11641 atccttacta ttcaaggata aggcgtctgc tctcatttga tctacaacaa tcaaaataat
    11701 atttggctgt tttgtctgaa ccataaaatc tcctttctaa tatggcaaaa gaggcacaag
    11761 aagatatcta cctttactgc acccctttct atatcaatct ctctatataa agcaataaca
    11821 ttcttgttat gttttataga acaatgaact aaaatatgac taaatcgatt aggaaattca
    11881 aatcattttc tagtactgtt ttagaagtta cagtgtacta ttccaacttc aacaaactat
    11941 aaaccttggt ctaataacaa ttttagtgga gataagaaat cctacaccta actcatctta
    12001 cacgtaatct atttctattt tatcacaaaa aacgcaagta agaccattaa ctcaattcag
    12061 ttttatctgc cattttcaca aatgggaaat aagtcaagac actaataatc aaacaaacaa
    12121 ctgataagat gatggcacgc caatcaaatg ctgtagagaa gaaaccatat aaaattggag
    12181 gcattaccca agtaacattt tgtgtaacag gtgaaacaag accccagctt gttgcccagt
    12241 aagctaccgt tgccatgaaa accgggctaa gtacaaatgg tataaatagc aaaggattca
    12301 agacaactgg taaaccataa ttgaccggct caccaatatt aaacagaact ggtgctagac
    12361 caagtttagc aacttttcga taatgactgt ttcttgaaaa aattaaaata gcaagtacca
    12421 atcctaatcc tccaaaccag acaaacgccc caaaagaccc acttgtccat atataaggaa
    12481 tcggttcacc tttttggaaa gcatccagat tcgctaacat agcaactcca aacagccctt
    12541 ccatgatggg agccaataca tttcctccat ggagaccaaa aaaccagaat aacttattta
    12601 aaaagatcat cagaataact gcaaagaaac tttgagacaa acctagtaat ggcgtttgta
    12661 acaccttgta aacccaatca atcaataagt cattgctaag taaatggaaa acataagtca
    12721 agatggctac tatatacatc gccataaatc ctggaatgat agaagtgaac ggcttagcaa
    12781 tcgcaggggg aactgaatct ggtaacttga ttacccagtt ctttttcatt actttacaga
    12841 aaataataga ggctaaaaat ccaatcatca tggctgtaaa gtagcctctg gcattaatat
    12901 ggtttcctgg aatcacattc ccaatagtta ccatcagatt tttaccatca aatgctagat
    12961 tatcaattcc ttgtaagatc tgatctaatt tcacatctcc tacatttgcc aaagggaaac
    13021 tctttgtaac tgtacttcca atcgaaatga caaacgaagc aagtgatacc aaaccagcag
    13081 aaactgtatc aaccttgtaa atcttagcga tattcactcc caagcaatag atgaacaaca
    13141 aagaaacaat tggtatactt cccttgaata ccaaattatt gatgtcaaca agccactgaa
    13201 aggttttcgt aatactttct aggtgaaatt gttgtggtaa atccactaga aaagcattta
    13261 ataacaaagc aatggaacct gtcataataa caggcatagt ccccacaaat gaatcacgta
    13321 aagcaactaa aaacttctga ttcccaactt tcattgcaat tggagaaagt ttttcttgca
    13381 ttttatccaa cattgtattc atactaaact cctaattttt ttcaaatcgt tcgtatactt
    13441 ctaccacttc tttcgccaag tcaacaaaag tcagactcgt cattaaatga tcttgcgcgt
    13501 gtaccatcaa gaggctaatt gatgtttctt ccccctgtgc ctctcttgtc aacatttctg
    13561 tttgagcctt atgcgcctgt aataaggcag cattcgcatc agctaatctt cgattggctt
    13621 tagagaaatc cccttttttt gctgcctgaa tagcctccat agcattactt ttggcttccc
    13681 caccatacat aattagcccc attacagatt ctaaattact ttcatccata acattctcct
    13741 caatcttagt ctaataaact ttcagctaaa tctaaaactt tagaaccatt catcattccg
    13801 taatctgtca tcggaataac cgccacagga atctgtctat cttttagttt ttcttgaaag
    13861 tcccctagta aatagcgaac ttgggggcct aaaagcaaca catttacttc ttttgttgct
    13921 acaatttctt ctgcttcagg agctggaact gcaaaaattt ctgcatccaa ccccttatct
    13981 tctgctgcct tttgcatctt tgtcactagc agacttgtac tcatacctgc tgcacacgct
    14041 aacataattg ttactttagc catagcttac tccttttatt ttattgtcaa tgttactgtt
    14101 tttcgatcaa ctttagactc ataatctaaa tgtttgggac gactagtata atgcaaatta
    14161 taaactcctg gaggcatttg tgaaatcttt tctttttcag ttttattgcg aattaataaa
    14221 tctaccgaag tattccctcc aaagttatta aacataggtt cattttcaat agaataatct
    14281 aactcatatc cactattagt tgtaattcct tttaataaat caggaacatc tcctctgttt
    14341 attactatat ccttagcgcc atttattttg ggagattgac tataatactc ttcagcttga
    14401 tgttgttgta aatcatttct agagtaagaa acataatcaa ctacaaattc agtccctgat
    14461 aaattattgt ccactacccc aggccaactt cctcccatcg ctaaatttaa ctgaacatac
    14521 tgaggtctat taaaaatctt tttcgctata ttatctgatc cataatatac ttttctaaca
    14581 atatgattgt caaccatcca tacaatataa tctttatacc aatcaattga aaaagtatga
    14641 tacgaatcat taaagtttcc actcgataat ttaaaggctg taccacgtcc tgcaaatttt
    14701 ccatcatcat tatccgactg tccataatga agcgtttgat aaactgtctt attcccactt
    14761 ccatttgaat ttcgttcacc aactaattcc attatatcta tctcacctgt tgacggccat
    14821 cctcgtccct gaacaggatt aatcttccca tcaagtgtaa aatccgatcc aagagtccag
    14881 aaagcaggaa aaactccttg acctttcggt aattttgcac gcatctctaa tttaccataa
    14941 agaaattcta ctttcccatg tgttctaaca cttcctgaat tatatacaac ctttctgtga
    15001 ttgtttcttg ggttaaaata ctgatcttcc ttagagcgat tcgtagctct aagaactaaa
    15061 tttcccgaac gtagaaaaac attctcatca cttcttacat aatgttgctg ttcccaacca
    15121 cgaattgatc ctaattcata tccccaatat tttttattta attgttcgcc atcaaaatca
    15181 tctctccaaa ttttcttata tgaatttcct ttagacagtt gatacatctc tacattatca
    15241 ataaaaatat tagatttttt tgtagtatcg tcttctgacc atttcactaa ttgaatcaaa
    15301 actttatcgt gtttaccgga attgaaagaa aacatgtatt ttttccattc ggttgtatta
    15361 actgtttgat caacagcttc tccaatgtta ttttgaacaa tctctccttg cttattatta
    15421 tcaaaccatc ttgcggtaaa atatccctct gctccactaa tatctacttt ggcaaaagca
    15481 gatactaaat aatcttcatt aggttctata tcaataactt gtccaatata tccatctgct
    15541 ctattatcat taattgcagt agaaggaatc atacctgagt aatgtccgtt actataactc
    15601 tcatttgaat taattattcc cctatttcta gagttccaaa acagctctcc ttgctcaaaa
    15661 tttgaatttt ttattaaatt ttcattagca aaaactttca catgaataaa tattaataaa
    15721 aataatataa ataaacttga aattaatttc ttattcttca ttttatcacc tatttactga
    15781 cctagccaag cgagatactg tatgtctcac tcctctaatt gccttgctca atgcataaat
    15841 attatcgact gttgccagtc ccccatatcc tgcgtcacct atatgttgaa tatcaactcc
    15901 acaaatttta tttctaagtg caatttcctt gatagtatct gtatcagatg tttcttggct
    15961 agtaccaata gcactcagta ctagcccacc cttactatga acgagatcaa cgacttcacg
    16021 caactcttgg tcatgaaagg ctggaacggt tccaactgct ggaacaagta tcacatccgc
    16081 acctgcttcc aacaattgct ctgcgacaga aagctctgcc acaggctcat tcactcctgc
    16141 cccgtgcatc ttacctgcaa taatcaaacc agaaaagttt tccttagcag tttgaacagc
    16201 cttaatgatt tctcgattgc taactcctgt tccaggattt ccagtcaaac agacaaagtc
    16261 aaaacctaac tcctctattc gattcaatgt ttcaacacta gcaacacgac ctgcaacaat
    16321 ttcctgtgtt tcctctagca tctttgcaga tgggtcaatc ggttccaaat ttacaccaat
    16381 tggacaagca acaagctggt gtaagcgatg aataacatca tcacctgaac tatccaaagc
    16441 ataaatttta ggctcaaaaa catcaacaca attcaataaa atcatatcag ctccaaaagc
    16501 tctagcaatt tcagaattag ttatatcccc aacaaatgtc tcacgagtaa ctacattttc
    16561 agatagaaca actctacctt cacttgctaa aatactttgt tttaattctt cagcattcat
    16621 ctgtaatatc tcagatggat ttgcactaat taaacgtttc attctcaatc ctccaataac
    16681 ttaaaccgtt tttcaatagc tattacaaat ggtaaataaa gtaagacatc caaaacaatc
    16741 aatacaattt gtaataatga accagaaata cttcctgtag caagtaaacc acttataaca
    16801 ggtggcatag tccaacttgg atctgtataa gttagcggaa ccaaacctga tgccattgct
    16861 ccccacgcta ctagtaagtt aaacattgga gctaagataa atggaactag taacaaaatg
    16921 ttcaaaacaa taggaacgcc aaacattgct ggttcattta tattaaataa tccaggtata
    16981 acagttatag gtgccaaagt ttttagttgt ttactagctt tcttcttatg tgctaaatat
    17041 cctaacgcaa gaactaaacc tatggttgca ccaccgccac ctatgaatac aaaattatcc
    17101 ataaatacgc tagtaaaaat atttggaaga gtttctccag tttgataagc tactttattt
    17161 gcatctagat tagctaacca taacggtttc ataactgcat taacaacatt tcctccgtgt
    17221 aatcctacaa accaaaaact actattaagg aaaattaaga taagaagacc aataacatta
    17281 tttcctaaaa gtccaagtgg acctcccaaa gctccttgag caatttcatg aagattaggt
    17341 aaacttaatt tatctaaagt tgcataaaca atcaaccaac caacaatagt cactgcacca
    17401 ggaataatgg cactaaagct tttagatact gctggtggaa caccatccgg catttttatc
    17461 tgtatattat gatttataaa ccattggtag atatagccat taatcaatcc taaaatcata
    17521 gcaacaaata aaccttttga tgccatgaaa gcggtaggca ttcctgctcc agcctctcct
    17581 gtaatgaaag gcgtaactgt aataaaagat gataatgaca caataccagc aggaatacca
    17641 tcaactttgt attgtctagc cataaagtaa gcgataccaa aactagctac aagaccaagt
    17701 aatccaaaac tactatcaac acccttccat aaatagtcag caacaccaat atcacctaga
    17761 tatttttccc atcctggaat aggaaaactt gcaataacca ttagtaatga acctatcaac
    17821 aataaaggta tagttagagt aataccgtct cgaatagaaa ccaacgcttt attattaccc
    17881 aatctagccg ctactggaag taacttttct tgtaataatt tttccataac cttttcctct
    17941 taaatatatt ttattatcaa aagttatcaa ttatctttat tttttgacgt caagttatta
    18001 tttgattatc ttttgttatt tatattttac aactataaga aagccttgtc aaggatttgt
    18061 gtaaatattt gattattttt tattatcaat atataataaa aagagaccag taagcaatga
    18121 aaagcttaaa aagattaaca tctacattta tccatagcaa aaaatcatag gattttaaga
    18181 atctgacaac taggaagaaa actaatatga ccttatctaa gaaacaatta caactaagag
    18241 ctaaaatact ggaaacagtt tacacattag gtcccatttc acgaattgaa atcgctacta
    18301 aaacaggaat aacacctgca acaactagca gtatcacaaa tgatctgatt aaagaaaata
    18361 ttctcttaga acttggtgaa gacgaacacg atacaagcgt agggagaaaa aaaatcctgc
    18421 tagacattca agctaaacga ttttactaca ttggttgtga actatctgaa aaacatttca
    18481 cttttgcttt aggagataat ctaggaaata tcttaaagga agagaaagaa attgttacta
    18541 aacagttaat ccaagaaaag ggaaatcaac tcattaacca gacactaaag caattcttaa
    18601 ataactgctc tgattatgaa atagaagcaa ttggaatcgc gctacctggt cgctatttag
    18661 atgattataa aataacaaca aacaatcctt tatggcaaca tatcgactta gaaatgattc
    18721 aatcccactt tgataagcct ttgtttttct ctaacaatgt taactgtatg gctataggta
    18781 aacgcctatt tagtcgccaa cagaatgacc ctaattttgc ttattttcat tttgctagag
    18841 gtatgcactg ttcttatatc tacgacggaa acatttatgg gaaaggaaat ctaatgattg
    18901 gagaaatagg gcatacagtt gtttcatctg agggagaaga atgtagctgc gggcgcaagg
    18961 gatgcttaca aacttttgct ggagaatctt ggttaatcaa aaaatcaaaa attctatatc
    19021 atcaatctcc ctattctcta cttcctagtt tagtcaaaaa tgccgatgat attgatatcc
    19081 aagttatttt aaccgcttat caattaggtg acactggaat aatcacactt atccatcaag
    19141 ccttactcta tcttagtcaa accattttaa atatcagtat gatgattgac tctcaaaaaa
    19201 tctacctaca tagtccttta ctaacaaatc aacacatcat ccaaaaacta tatagtgaaa
    19261 tgaactacaa acccaaactg ctctataatc gcctacctga agtcattatt gaaccttaca
    19321 atgattttac agccgcacac tcagctattg cactttgttt gtatcataca attctacatt
    19381 cttaattttc tattacattt aataaatagg ctaaaaagag ggagtatatc ataaaaagcg
    19441 aataaacctg ttttctgaaa atcagaattc tgttttgttc gctttcttta tatttggaat
    19501 tatactccta tctccccttt ttcataatgt atagtgaaat gaaataataa caggacgaat
    19561 cgatcgggac agtcaaatcg atttctaaca atgttttaga aatagaggtg tactattcta
    19621 gtttcaatcc actatatttt cgtacaggtg cttcaaccat ttgaacgatt tcaaatcctt
    19681 ctttttggta aagattctga gctctttgat ttgcctcgaa gacatttaga gaaatactat
    19741 ctatatcttc attttcaaag gccaaactaa caaatttcct taaagcctgg ctacctaagt
    19801 cttgctcctg tttctggggg ttgataaaaa atctcccgat atgaagattg ctgtcttcta
    19861 gcctgatttt ctggataagt cccacaaact cttgttcatc aaagattgaa aagactcctt
    19921 ccaaggcttg aaggggcagt agaaaaggaa tccttggtcc catccattgt tcttgaaagg
    19981 atttgcctag ggagttggac cactggcata caaattgagc gttttctata ctcacctttt
    20041 cttcaaaacg aattgtcatc ttttcctcac catcttatct atgtttctcc attatactat
    20101 ttctcccatt ttttacgaat agataagtat gattgatttt tatttttttc tcgtcgggag
    20161 cattctcgct tcctttcttg gtttggtcat tgaccgtttt ccagagcaat ccattatcag
    20221 ttcagccagt cactgcgatt cctgtcagac tcccttgcgt cccttagatt tgattccgat
    20281 tctctcacag gtcttcaatc gctttcgctg tcgctactgc aaagttcgct atcctgtctg
    20341 gtatgccctc tttgaactag gcttaggact cctctttctg ctttactctt gggaattgct
    20401 ttccttgggg caagtcgtcc taatcaccgc tggtttgacc ttgggtatat acgactttca
    20461 ccatcaggaa tatcccttac tggtctggat gactttccac ctaatcctaa tagcttcctc
    20521 tggctggaat ctggtcatgg tctccttcct catacttgga attttggctc attttattga
    20581 tatccgcatg ggtgcagggg atttcctctt tttagcttct tgtgctctcg tctttagcgt
    20641 aacggagtta ctgatcttga ttcagttcgc ttctgcgacg ggtatcctgg cctttctcct
    20701 gcaaaagaaa aaggaaagac ttcctttcgt gcctttcctc ttacttgcta cttgtgtgat
    20761 tatttttggt aagctactac ttgtttgata aattccaatt tctgccatat gtactccatg
    20821 aaattatttc acagttaaat tataaattat ttcttttgta caaagggatg atgttatcaa
    20881 atcgatctgt tcttctatct tcttgatact gatcaaaaaa tttcatttcg actgaaaata
    20941 tttcgcttat aaactgtaaa cgaatacttt gtttagacat tatagtcgct agactgacta
    21001 gatgattact caaaacgacg tccagaatac tctttacttt gcttggtttt ttaacaaaaa
    21061 tttgatcatc caaaggttca atcattttgt aacctttttg cgcaatttga cgataaaagt
    21121 aagaatgttg ctttggagtc aataatccta acttaaaagc tcgatactct aaagcctgta
    21181 tcgaaacatt caaatccgac ttcaataaaa tataactatc aggattgctg acacgcttgc
    21241 caaccctctc ttcaaatttg actaaaaact cttcttttgg caataaaaaa catgatgcaa
    21301 aataatttgc ttcttgctcc aaacgatcgc catcttcatt catatcttta tatttatgta
    21361 aaagaatatg tcctagctca tgagctaaat caaaatttcg acgtacagat gatttattcg
    21421 ttcccaacac aatataaggt cttcccaatt ttgaccatgc gctataagca tcagcttggc
    21481 cattaattaa tcgttccacg atatagatgc ctgaacgttc taatttataa agcaaatcat
    21541 gattatcttt tgaaatacct aatttttccc tggcataaag agccaattcc tcaatggatt
    21601 ctcccttatg ataagattca ctcactacat tacttaggtc atgaattgta atattaggta
    21661 taattacaaa actttcaaaa taatcaatca aactatctac cttatgtaaa tacatagttt
    21721 gaatatctat tgttttccgg gttgctaggt ctgcatttct aaaggcaatt acagaagaat
    21781 caaatcgaat gctctcttct tcctgttcaa aataagttaa atcaacatga aattggttgg
    21841 ccaaatgcat tttggttgat aatttaggtt tcgtttcgtt ggactcaaac tgccaaatgg
    21901 cttgttccgt taaattaatt ctctgagcta attctgctct acttaaacca tttaacagcc
    21961 gtaattcttt caatacccga ccattaaaca tttacatact ccttactact tttgaccttc
    22021 ttgtttttct attcttggaa taatttcaaa atcttctgtt tccgataatt ctgaaaaatt
    22081 aggaatatct tgatatttag cttcttcgaa atggtacgaa cttgattgaa tatactcaga
    22141 caaatcttga atcaagtgta actgaccagt tcgtgcatca ggcataacaa catctacaga
    22201 tataatattg ttttctgagt ccgcctcata agttaaaatc ataaattttt cgatattcga
    22261 atttttagta gcttgttcaa tttcttgaat catttcatca gaaactaact ccatctgaat
    22321 tggaaaggaa tgactatctt catcattttt gtaggaagaa tgttgattaa gataaagtgt
    22381 attcatctga gcatattcaa ataagtagcc actcttattt ttttgtacca aaggaaattg
    22441 gttcgtaagt cgcttcttac cctttataat taacaatact ttcccatatt tttctgtgtt
    22501 tgtttcaaat tctaaatatc cccaagtctg tcctgctaat tgtaatttat actcaaacaa
    22561 atctgctgat gcaaatgcag tatcaatatg attaggtcgc gtccatgcat aaccattcga
    22621 cactatcatt gtctctcttt tttctagacg ttcatctaca taatcttttt gccctttcat
    22681 caaagtatct acaatttttt gtgcctcaag cgaatcaaag agatcctgat tcaacataat
    22741 tcttcctcct ccaaatactt tttaatgaat tatatcattt tcttaaagaa attactacaa
    22801 taattagctt tttcttaaag ttctgtgtca gagtaattta gaaaattata tcttctatag
    22861 taaaatcaat taaaaactga acaaatttat tgggaaattc aaatcacttt ctaaaaatat
    22921 tttaggaacc gtagtgtaat attccagatt caatttacta taaaactagc ctttctcctg
    22981 caaaagaaaa aggaaagact tcctttcgtg cctttcctct tacttgctac ttgtttaatt
    23041 atttttggta agctactgct tgtttgataa agtcctgaat cggctctcct tggtggagag
    23101 cttttactat tttcgaaccg acgataacac catctgacac cgcattgaag cgttccagat
    23161 cggcttgact agatacacca aaacctgtca agactgggat gtcggccact tgatgaagtt
    23221 gcgccaagtg cttgtccaaa tctgcacggt aattgcctga tttccctgtt accccattga
    23281 tggcaacggc atagacgaat ccctccgccc cttcaatcaa ctctttctgg cgctcaattc
    23341 ctgtggtcaa gcttactaaa ggaatcaagg cgatatctgt atctgccaaa aatggttcta
    23401 caaagttggc atgttcatga ggcaggtctg ggataatcaa gcccttcaca gctgtatcag
    23461 ccagatcttt gacaaagttc tccacaccgt actgaaagag ggggttgaag taggtcatga
    23521 tgaccagtgg aatctctgtt tcaatggttt tcaaggtttc aactaaagcc tgggtagagg
    23581 tcccgtgggc taaactgcgc aagccagctt cttcgataac aggtccatct gcaacagggt
    23641 ctgaaaaggg aatacccact tcaattgcag agacacccaa atcttctaaa aagtgaattg
    23701 tttcagcaag accatccaaa cctttttcgt ggtcaccagc catgatatag ggaacaaaaa
    23761 ttccttttcc agctgcttta atagcattta atttttttgt tagtgtctta ggcatgagct
    23821 tctcccttct ttgctgcatc tgcttccaag cggtctttta cttgaaccac atccttgtcc
    23881 ccacgacctg ataggcatac aatcatagac ttttctggtc caagttcttt ggccaatttc
    23941 accgcaaagg cgatagcatg gctagattcc aaagctggga taatcccttc cacacgagac
    24001 aagagttgga atccttccaa ggcttcttcg tctgtcacag ggacatagct ggcacgttta
    24061 atatcgtggt agtgagaatg ctctggaccg ataccaggat agtccaaacc tgctgagata
    24121 gagaaggctt caagaatttg accatgggca tcttggagca catccatgag ggaaccgtga
    24181 aggacacctg gacgaccctt ggtcaaggta gctgcgtggt gctccgtatc cacaccaaat
    24241 ccagccgctt cagctccata catggctact gactcatctt ctacaaaggg atggaagaga
    24301 ccgatggcat tcgacccacc accaacacag gctactaggg catctggcag attttgtcct
    24361 gtcaagtcac ggtactgttg tttagcctct cgaccaatga cactttggaa gtcacgaaca
    24421 atttctggaa atggatgagg ccccaaggca gaaccaagga tatagtgggt atcatctata
    24481 ttagccaccc atgaacgaag ggctgcattg accgcatcct tgagcacgcg cgaaccatct
    24541 gtcacggcct cgaccttagc tcccaaaagc tccatacgga agacattgag ggcttggcgt
    24601 ttgacatctt cctcacccat gtagatggta cattccatgt taaagagggc cgcagcagtt
    24661 gcagttgcca caccgtgctg accagcacca gtttctgcga taattttctt tttacccatg
    24721 cgtttggcaa gccaaacttg tcccaaggca ttgttaatct tgtgggctcc tgtatggtta
    24781 aggtcttccc gtttgagata aatcttggct ccgccgatat gctgggtcaa gttttttgcg
    24841 taataaagag gagtttcacg tcctacgtac tggcgcaaaa gctggtttaa ttcctcttgg
    24901 aaacttgggt ctgcctgact ttcacggtag gccttctcca actccaaaac tgctgtcatc
    24961 aatgtttctg ggacaaaacg tccgccgaat tttccgtaaa atccatcttt atttggttcc
    25021 tgatatgcca tgctttaccc tctctataaa tcttctaatc ttttcatgat ctttttgtcc
    25081 atctgtctcc actccgctcg atacatctac tgcataggga gtaaaatgtt gaattgcttt
    25141 tactacatta tcttcattaa ggccacctgc gataaagaag ggctgtgcta gtccagtcgt
    25201 atccagttga ccccaatcaa aggactgacc acttcctgcc acaggggcat caaagagtag
    25261 ataatctgcc tgagaattgg ggacatgccc atttccatct acctgcacag cctgaatact
    25321 ggcacaaggc aaattctcaa ataaatcagc tgccacctga ccgtgaactt gaaccaagtc
    25381 caagccaact ttgtcaatcg cttccagcag ttctacccga cttggtgaaa caaatactcc
    25441 aacctttttc acatctgcag gaataagctt tgccaactca gctgcctctt ctaaagtcac
    25501 ctgtctttta ctaggtgcaa agacaaaacc gatatagtcg gctcctgctg aaacggctgt
    25561 ttccaccgct tctttggtcg atagtccaca aattttaacc tttgtcaatc tgcaactcct
    25621 tgattctctg ggccacattt tctgcctgca taagagctgt ccctaccaaa attccgttaa
    25681 agtatggggc tagtcgttcc gcatcctgcc ctgtgaaaat ggcagattca gaaatgtaat
    25741 agcgaccttc ctcaaagtaa ggggctaaat ctacactggt ctgcaagtcg acctcaaagg
    25801 tagtcaagtt gcggttgttg accccgataa tctcagcacc aagtctgtgg gctacctcta
    25861 gttcagctag attgtgagtc tccactaaga cttccagacc aagctctgtc gcgtagtcat
    25921 acagttcctt gaggcgttct tcggacaagg ctgccacaat aagcaagata actgtcgcac
    25981 ctgtattgcg agcgcggatg atttgctttt catctatgat aaagtctttg ttgagcgtcg
    26041 gaatctctac ctgactggaa atttcccgta gataatccaa atgcccttta aagaaaacct
    26101 catctgtcaa caccgaaatc atcactgctc cgttttcttc ataagtctgg gcctgttgca
    26161 caatatccac atcgagattg atatctccca aactagggct agctttcttg acctcagcga
    26221 ttacctgcaa gcagtcctga tgattcttca aaaattctgc caagcgatag gtctggcgca
    26281 gaggctggat ttgctccagc ttcatctgct ccacctcacg cgccttctgc tctaagattc
    26341 gtgctaaaaa ttcctgactc atttttggta ctcctgtaac agtctgagtt tttcaagggc
    26401 cttgcctcta gcaatcactt gacgggccaa ggcaactcct tccttgatgc tatcaatctt
    26461 accattagca tagaaaccaa gaccagcatt caagactgtc gtttccaaga atggacttgc
    26521 ttcgtttttc agaacgctaa gcaaaatttc tgcattttcc tgagcattcc caccacgaat
    26581 atcttccata gcatagtctt ccattcccaa atcctctgga gtaaagcttg acaagctgat
    26641 ttcgccattt tcaagaagtg caatcttggt tgttccgttc aagccagctt catccaaccc
    26701 ttctggtcca gcaaccacga tggcacgttt gcgacccata tttttcaaaa cctgagctgt
    26761 actttctagg agttctggac gactaattcc aagaagctgt gtttctaaag ccattggatg
    26821 aatcagtgga ccagtcaagt tcataatcgt tggaattccc aattccaaac gagctggcat
    26881 gatgtatttc atagctgggt gcatattttt agcgaagaga aagacgattc cagttttatc
    26941 aaagacctta cctagttcag ctggtttgag gtcaagattg attcccaagg cttcgaggac
    27001 atctgcggaa ccagatttag aagaaatcga gcggttacca tgcttggcca tgtgaatacc
    27061 gccaccagcc aagacaaagg ctgcagttgt ggaaatatta aaactgaaag acttgtcccc
    27121 acctgtacca cagttgtcca tagcatcatg aatctcagtt ggaatatgct gggcatgtcc
    27181 tctcatgact tgggcaatgg ctgtacgttc ttcaggtgtt tcccccttca tcttaagagc
    27241 taagaggaga gaagcaatct gcgcttcagt tacacgccca gttacgatac gctcaatgac
    27301 atccgtcatt tccacacctg ataaattttc aaattttgct agtttttcaa taatctcttt
    27361 catcctagtt tcctcacttt acaaccttct cgataaaatt ccgaatagaa gacaagccgt
    27421 ctggcgttcc aatgctctct ggatggtact ggaagccata aatcggtagg tttttatgtt
    27481 gaatccccat gatggcttgg tcatcagtcg aacgagctgt cacttcaaag tcttctggca
    27541 tttcctcaat caaaatactg tgataacgca tgaccgcacg tccatcctca ataccttgat
    27601 acaaaacaga tggcgcttca aagttgatat tgctctgttt cccatgcatg acttttggag
    27661 ccaaacctag cttaccacca aagacttctg caatggcttg gtgacccaaa caaatcccta
    27721 gaatcggctt cttgcctgca aaatcacaaa tcatgtcttc catctttcca gcatcaactg
    27781 gccaaccagg accaggagaa aagaccagac catctgcttt ttcagcttct tcatacagct
    27841 tggaatcatc atttctcaaa acctgaactt ctgcaaaatt cccaatgtat tgggccaagt
    27901 tataggtaaa agaatcatag ttgtcaatca ataaaatcat ggtcttagtt ctccaattct
    27961 agtcatagat tttgctttgt taatggtttc ttggtattcg ttttgggcga tagagtcgta
    28021 gacaatccct gccccagcct gcacataggc tctttgattt ttgagaatca tggttcggat
    28081 ggcaatggcc aaatccatat cacccgtcgc agacaagtag ccgattgctc ctgcatatac
    28141 gctccgtttt tccgtttcca gttcatagat gcgtctcata gctcgaattt ttggtgctcc
    28201 agaaacggtt ccagcaggaa gcgttgcttt caaggcatcc atggcagtga gttctgcaag
    28261 caaacgtccc ttgaccacac tggtcaaatg catgacgtag cggaagagct ccacctccat
    28321 atacttagta acttggacac tggccgtttc agagatgcgg ccaatatcgt tacgccccaa
    28381 gtctaccaac attcgatgtt ctgctgtttc cttctcatca gagaggaggt cagtcgccaa
    28441 ggccttgtct tcttcatccg tagcccctct tggtcgcgtc cctgcaatcg gattggttgt
    28501 cacgatgcca tttttgacag aaaccaaact ttctggacta gctccgatga tttgataatc
    28561 cccaaaatca tagaaataaa ggtaattaga tggattagtc acgcggagat ttctgtagaa
    28621 gtcaaatgga tttccagtaa cttctgctga gaaacgctgg ctgagtacac attggaacat
    28681 atctccgtta cgaatcaagt cacgagctgt ttctaccatt ccctcaaact tatgtggagc
    28741 gatatgcggt ttgaagtcaa gtggtgataa atccaagtct tcaaattcat ttggagcagg
    28801 aatgcgtaat tcctcaagca cttggttcaa ggatttttcc aaggcctctt gactgcgctc
    28861 actataaagt gcatcctcta tgacatgtat cttctccttc ttgtggtcaa agaccatata
    28921 gctctcatag acaaagaaat gcatgtctgg cgtcccaatt gtatcctcag ggatttgacc
    28981 aatttcttca taaagcgaaa tcatatcgta accaacaaaa ccaatggctc ccccaccaaa
    29041 agggaggtct gaatggtgct ggctcttatg agtcacttca taaaggaaat ccaagggatc
    29101 ccgatcaatc gcttgaccat tttgatagag aactccattt tcaaacttaa tctcaaaaac
    29161 tggattatag gctaggatag aaaaacgagc tgtttccttg tctctcggaa tactctctaa
    29221 aataacctta tgttgcccct ttaagcgcat ataagccaag attggtgata agacatctcc
    29281 atgaatgatt cgttccattg tcatttccct ttcagttcta attctagttc gtggcgactg
    29341 tatgaaaaat ccccacgcaa aataacttgc gtgaggacga aattcgcggt gccacctcaa
    29401 ttataggatt tctcctatct ctcattcctg tctcagatat ctcctgtaac aggctgtgcg
    29461 ataaagggca ctcccttgag aatgatgttt tcttctctcg tttcagatga acccaacttt
    29521 acagctttct ctgcttgttt tcagcaacca caagctctct gtgagagaaa agactgtaat
    29581 ttttccatct attatttttt agcttctagt aatctgcaat cgcagctagg tccttgcctc
    29641 cacgaccaga gacattgatg aagagatgtt catctcggta cacctttata ctcttcgaaa
    29701 atctcttcaa accgcgtcaa cgtcgccttg ccgtaggtat ggttactgac ttcgtcagtt
    29761 ctatctgcaa cctcaaaaca gtgttttgag ctgacttcgt cagttctatc cacaacctca
    29821 aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt gctctttgat tttcattgag
    29881 tattactagc ttttttcgta ttagtccagc ctttttgttt acttttagta ggaggcaagg
    29941 agctgtagat agaactcaag ttcatcaaag cgacttaagg ccctaataaa agataaacca
    30001 aacgacggat agaaaaaagc ccacacacag aatatacttc cgtgtgaggg cgttggtaac
    30061 gcggtgccac ctcaattata aagggactat ccctttacat ctctgccttg tttaacaaca
    30121 agctgcactg taaggtgtgc gcaccgaatt ttcattgttt caaattcatt ttcaaaatca
    30181 gcccactttc actacttcca accacctatt cacaatcacc acaggctccc tgaagatcaa
    30241 aaatagttac ttttctgatt tgttgaactt attttaatac tttgtttttt ctttgtcaag
    30301 acttttttac gatttttttg atatcattcg aatgtgacca tatcttcctt agatcgaaca
    30361 tgaacatgtc ccacttgtta gaaattggat ccaactcaat agaaactgaa tggaggctaa
    30421 acagaactta ttttagaaca ctccatcttt tccactagga ttttcaagaa ttaaacaata
    30481 ctagaaactc tgtctcctaa caaatttagg agaaacttca acagatgtga cactttcccc
    30541 tttaataatt gctaaaacac cttctatcat ttctttagcc aatttaacat aattgggagc
    30601 aattgtagac aaagctggag tataatactg agaaatagga atattatcaa atccaatgat
    30661 agaaatatca tctggaataa gaattccttt ctcatagcac gcacgaatca agccctgaac
    30721 cttttcatct cctgaaacaa aaataatgtc cggataattt tgggtagtca agtgctgcat
    30781 tgcataagaa taaactgaat caattgtaga taagccataa atgactttta aatccataaa
    30841 gtaattttta tcattcagaa aagaacgcac acctctttca cgatccttat taacatggga
    30901 ttctcctccc ataagcaacc acatattttt aaatttttct tcagttacag ctttcatcat
    30961 atcataagta gcttgaaaat tattattaga tacatagact actccagacg tttgagattc
    31021 accgaaaaca agaaaaggca tatggttctt ctttaaatac tgaattctga tatcatctac
    31081 actttcataa aaaacaataa caccatctac taggctacct gtgcttgata taattgaatt
    31141 actaattgta tcctcctctc caaagtactc aactatagca ttaacaccaa attctttaca
    31201 cgtccgtaac actttatcta acagcgtatg aaaccaaatt aaaggaaaag agtcgatttt
    31261 ttttacagaa atcaatatat ttatagcttc ttttttagtt aaattttttg catacgcatt
    31321 tggaatatac gacaattcct ctataacttt ttgaatcgct tgataagttt cttctttaac
    31381 atttactcca ccattaataa ctcgtgaaac tgtttttgga gaaaaacctg ataaacgtgc
    31441 aatatcataa atagttacct ttttcccatt tatatttttc atttcagtcc tccattacga
    31501 acattctaat attactatac aatatttaat tttttttaac aagagaattt agtaaattat
    31561 ttaagatcca caaattcaca aaattaattt tacaaatatt cttccccttc aaaaaaattt
    31621 aaattgcatt tcacaccttt atttttaaga atgtttccaa cttcacgaca aataaattca
    31681 tatgagaaaa aactgccata aaattgtaga ttaacttttt cagtaaaatg tgtaggattt
    31741 ataaaaacat ataatagcct gtcaatgtaa cattttaaca tagagttaat tttttcttta
    31801 aagataacat ttgttatcaa ctcatcagga ggtaaatgaa aggcaaacac catttcacaa
    31861 atatcataaa aagaaataaa tttgtatact tgtatcaaac aattattatc aaaatattct
    31921 attttaccta aatcaaaatt gattttataa tctttcataa aaacctctga gcaaaaatct
    31981 actcaaaaat tagatgatta aaacatctaa aaaacaaaag gacaaaaaca tctgtccctt
    32041 tgtttactaa atttcagcta atttcttcga cataaataac acctacaata ttagcaattt
    32101 cttccatcag tcgaagatgt tcaaatctac ctgataattc cagagtaata aatgacgcta
    32161 tttttttatc tggaacatca aagtattcaa ttctgtcaga attaacatct ccaaacgctg
    32221 ttcttgaatc ggtcattctg ataccatttt ctgcacaata aaccaataca cgattatagg
    32281 cttctgtaga tttaaccact atatacaatt caatcatttt agaacgattt tgtagatatt
    32341 tttttagtgg ttggaacatg gatatcacac cccaaacaga aatggctact aaaagagctc
    32401 cctcataaaa acctactcca atagctaatc caattcctgc cgaagcccaa atgcctgctg
    32461 cagttgtcag acctgtaatt ttctttttat ctgtaataag aatcgttcca gcgcctagaa
    32521 aacccacacc tgatataact tgagctccta atcgtgtagg atctcctgtc ccaaatttat
    32581 aagatacgta ttcattcgtc atcataatca aacatgcagc tagacaaaca atactataag
    32641 ttcggatgcc tgcaggctgg gatttgctcc ctctctctaa accaattata ctaccaatga
    32701 ctactgataa aacaatcctg acaactattt caatatttga taacccaaga ctagtggctg
    32761 tcatgattat ttccttactt tacgccccgg tctttgtgtg aagtataata ccgttccaga
    32821 aataatcatc agaacaattg tataaacaaa taccagagct tgtgcattag atgttgctgt
    32881 ttcatcacct gcagatcgaa tcgtaatacc taatggttga gctaggggat ggtaaaggaa
    32941 tacagataag tcgaagtcag ttaataaaga gttaaagttt aaagcaataa cagagagaac
    33001 aaccggtaaa ataaatggaa tgataacctt catcatagta taaaaaggtg aagcacccat
    33061 acttcttgct gcatcttcca tctcatcatc aacactaaat aaaatagcac gtaccattct
    33121 ataagaaaat gggattttta caactatata tgcaataagt agaattacca aactacctac
    33181 caaaatctga ttcaagacaa gaaattgtgg ctgattaaaa gtaaataata aacttactgc
    33241 taaaagcgta cttggtaata accaaggaag tagagcacca tattcaaata agaaatcaaa
    33301 acgagattta tgttttctga caacacgagc aaatacaact gcgagaattg ttgctgttgt
    33361 cgcagcaata atagaataaa taaagctgac caagaatgga gagaatgccg cactattact
    33421 aaagaataag cgataatttt ctaaagtaaa gtttgataat gttaagttac ctgtttgaat
    33481 tgcaactgga tctgtaaatg agtataatac tataaaaatt agtggaagca tgaaaactgt
    33541 gaacaatcca tatgctacaa tgtgagcaat gatattccaa ggcttagacg caattttttg
    33601 ttttttaaga ggcgctttag tcttagagat agaaatataa tttccacctt tttctatctt
    33661 attcatgata gtaagcaaaa ttgtagttgc aatacctaaa ataattgcaa gtaaggcagc
    33721 taaatcacga gaattcccca tccctgcaaa tgtaataatc attggattta tagtttgaaa
    33781 ttctttacca ccaacaatca tgggtgctgc tactgcagat aaaccactaa gaaaaaccat
    33841 aatagtaagt gcaaatagag ttggaattaa ggttggtaac actacttttc ggaaaacagt
    33901 aaatggtttt gctcccatat ttcgagcagc ctcaatagtg tgatagtcaa cgcttcgaat
    33961 tgtatttgtt aaaaacaatg tatgattagc agttcctgaa aatgtcataa tgaataagac
    34021 tgcaccatac ccaataaacc agttagggtc taaagaaggg ataacatttt gtaaaaattt
    34081 tgtaatcaat ccatatggac catagacaaa tttatatcca gtcgctaaaa ccactcctcc
    34141 ataaattaaa gaggtcatat aacctaattt taaaatttta gcacctttaa tatcaaagta
    34201 ctctgtaaat agaacacaaa gaatacctac gacattaact gtaataatga gtgaaaatgc
    34261 taacttaaaa ctgttcataa tactctgaag tgccctctga gattttagaa cacgatgtac
    34321 agcatcaagg gaaaattctc ctccttttac aaatacattc actactagat caaagtttgg
    34381 ataaataata aatgttacta agaaccagat taaccctaaa cgaataagcc aatcttttaa
    34441 atttaattta tgacgcatac tgcacctcct taaaattgca gaacgtctga tggtgtgata
    34501 aataattcca cactttctcc gacagatcta atagcagcct gactatcaat acttgttaca
    34561 ttaagaatct gactttcaga aacttttatt gtatagtgaa ttgtaactcc agaaaactca
    34621 acatcaataa ttgtcccttt tagaatgaaa tcttgttcag tttcacgatt gaatcgaact
    34681 ttctctaatc gaatgtatcc ttttttatct tctaagaaaa cgcttgtatt tttcagtaat
    34741 acttcgtgga ctgtttcatc ggtcaaaaca ttaatatctc caataaaatc acatacaaat
    34801 tcagtttgag aattatgata aatctctact ggtgtaccga cctgttcgat gtatccattg
    34861 ttaaagactg caattctatc agataaagtc aaggcttcct cttgatcatg agtaacatat
    34921 aaagtagtaa tacctaactc tttttgaagt cttttcaact cttttctcaa atctacacgt
    34981 aattttgcgt caaggtttga caatggttca tctagacaaa gaattttagg ttcaagaacc
    35041 agagcacgag ccaatgctac cctttgttgt tgacccccag ataattctga tacattacgc
    35101 tgtaactgtt gatcagagat cttaattttt gctgccactg ctgatacttt agctttaata
    35161 acatctggag ctaccttctt aacttttaaa ccaaatgcaa tattatcaaa aacagtcata
    35221 gttggaaata gcgcataaga ttgaaataca ataccaattc cacgcttttc aggttccaaa
    35281 tgagtgacat ctgttccatt aacttcaata cttcctgatg atggatctaa aaaacctacc
    35341 aatgctctca aagtagttga tttaccacat cctgaaggcc caagaaatgt aaagaattcc
    35401 ccttcatgta tatctaaatt cagattatca attgcaacaa aatcaccata tttaatttga
    35461 atattatcaa atttaatcat ctcactaact ccctctatta ctaaaccaaa agcctctctt
    35521 tatttcttcc ttaaatttag aaataataga gagacttgga cataaaaaat aactcttatt
    35581 tcttatttta cgtattctaa ttcagctttt tctacccatt catccaaatg ctttccaaca
    35641 gcttcccagt caatattttg tggtttcact tgatcaacaa attttttcgt atcttcaggt
    35701 aactctttta gggcatcttt atttgcagga atagatccaa attttttact atattctact
    35761 tgaatttctg attgaccaaa ccaatcaata aattctttag ctaacgcttg ttttttacta
    35821 gtgcttaaaa ccatagtttg ttcagttaca aatggtacac caatctcagg agtcataact
    35881 ttgaattcaa cattttgttc tttttgtccg attaacgctc cagaacccca gatcataccg
    35941 tattgaattg ggctttcttt atctaacgct ttaacaactg agctttcacc tttttgaaga
    36001 gtataggcat ttgcaaagta ttcttttgca acttcccatc ctttttctga tacgcctaaa
    36061 tctccttttt catcaaggta acgaactaag atacttgcta gaattgcccg tcctgtacca
    36121 ccttgaagac cataaattga atatttacct ttatacttac tacctaattc agtccaatct
    36181 ttaggcattt cttttacatc aggcgcccca attaaaacta atggttgaac aatcacagga
    36241 ttataataat tatctttatc tgataaagat tgatcaattt tatctaacca tttaggcttg
    36301 tactgtacta gtaatttttg atctctaatt ttatttgagt caacagcacc aattccaaat
    36361 accatatctg caactgcatt attcttctca gcaataacac ggtctgctaa ttgagcgcca
    36421 gcaatatcaa ccatttttat attaaaacca gcttcttttg ctttagcagt taaccaatca
    36481 ccacgaccat ttgagactga gttcgaatag attactaatt cttgactttt atcagctttt
    36541 tcttcagatg aagaagcagt cgtagaattt gaacctccag agcaagcagc aagtgtagta
    36601 agagcaactc ccgttgcaag tacagtagac caaactttca tttttttcat gataagttct
    36661 ccttttttat tattttattt aaatttttcg tgatatggaa caaattgtct catatcttca
    36721 aatacagtat agtcaatacg gtttacagta atagttggaa tcttctctaa taaaatttca
    36781 gttaattctg ctctgacttt agtaaactct tcttcctcct cttcggttag aggaatccga
    36841 agatacccaa ttgaaatatg gaattgatat ctatcatgat tagggaaaca aacacctgct
    36901 ttttctgaga cataagtacg aatttcttct aatctctttg cagaagcttc atctgcaggt
    36961 tcaactagta tgttttgttt tcccatttca gttatacgca tatgaatttc ttcatccaac
    37021 aatggaaaaa tttcaagttg tttagcaaag taatcatgta tttcctgtaa aggtgtatct
    37081 agaggaagat tactgctcca aaactccgtt tcacgatttt catggcacaa caattcaatt
    37141 acagtcatgt gaatagaatt ccttggagtt aaagtaaact tatcgataaa tggtaattct
    37201 ctataacgtg attgaataat atcaacaact tccatcaaat cttgtttagt ataaagattt
    37261 gctacaactg tattcccagg gaaatgatta aattccccat tctctttgaa cttaattttt
    37321 gttaagtttt tcatataaag accttctttt ttatatttga catcggtgtc attgtgttta
    37381 cattatacaa tgaaaccgtt tcctttgtca agcgctgtca aacatttttt ttaatttatc
    37441 tttcattaaa ag
//
LOCUS       DAVBCV010000013        37258 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBCV010000013 DAVBCV010000000
VERSION     DAVBCV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.013"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            200..1963
                     /gene="aspS"
                     /locus_tag="VQ516_000672"
     CDS             200..1963
                     /gene="aspS"
                     /locus_tag="VQ516_000672"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2511156.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVVNATLGTLRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            1941..2882
                     /locus_tag="VQ516_000673"
     CDS             1941..2882
                     /locus_tag="VQ516_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2511157.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(3002..3082)
                     /locus_tag="VQ516_000674"
                     /pseudo
     CDS             complement(3002..3082)
                     /locus_tag="VQ516_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3218..4204)
                     /locus_tag="VQ516_000675"
     CDS             complement(3218..4204)
                     /locus_tag="VQ516_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2511158.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(4214..5014)
                     /locus_tag="VQ516_000676"
     CDS             complement(4214..5014)
                     /locus_tag="VQ516_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2511159.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRIYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(5275..6117)
                     /locus_tag="VQ516_000677"
     CDS             complement(5275..6117)
                     /locus_tag="VQ516_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2511160.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(6119..7411)
                     /locus_tag="VQ516_000678"
     CDS             complement(6119..7411)
                     /locus_tag="VQ516_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2511161.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGNKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(7522..8793)
                     /locus_tag="VQ516_000679"
     CDS             complement(7522..8793)
                     /locus_tag="VQ516_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2511162.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            9343..10860
                     /gene="malQ"
                     /locus_tag="VQ516_000680"
     CDS             9343..10860
                     /gene="malQ"
                     /locus_tag="VQ516_000680"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2511163.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            10886..13144
                     /locus_tag="VQ516_000681"
     CDS             10886..13144
                     /locus_tag="VQ516_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET2511164.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(14188..14760)
                     /locus_tag="VQ516_000682"
     CDS             complement(14188..14760)
                     /locus_tag="VQ516_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511165.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAINLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(15382..16230)
                     /locus_tag="VQ516_000683"
     CDS             complement(15382..16230)
                     /locus_tag="VQ516_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2511166.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            16375..16653
                     /locus_tag="VQ516_000684"
     CDS             16375..16653
                     /locus_tag="VQ516_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511167.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            16655..18718
                     /locus_tag="VQ516_000685"
     CDS             16655..18718
                     /locus_tag="VQ516_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2511168.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(18796..20052)
                     /locus_tag="VQ516_000686"
     CDS             complement(18796..20052)
                     /locus_tag="VQ516_000686"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2511169.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            20194..22659
                     /locus_tag="VQ516_000687"
     CDS             20194..22659
                     /locus_tag="VQ516_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2511170.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            22724..23635
                     /locus_tag="VQ516_000688"
     CDS             22724..23635
                     /locus_tag="VQ516_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2511171.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCLEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            23865..24563
                     /gene="dapD"
                     /locus_tag="VQ516_000689"
     CDS             23865..24563
                     /gene="dapD"
                     /locus_tag="VQ516_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2511172.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            24631..25761
                     /locus_tag="VQ516_000690"
     CDS             24631..25761
                     /locus_tag="VQ516_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2511173.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFTSQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            25773..26312
                     /locus_tag="VQ516_000691"
     CDS             25773..26312
                     /locus_tag="VQ516_000691"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2511174.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            26296..26973
                     /locus_tag="VQ516_000692"
     CDS             26296..26973
                     /locus_tag="VQ516_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2511175.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(27045..27944)
                     /gene="galU"
                     /locus_tag="VQ516_000693"
     CDS             complement(27045..27944)
                     /gene="galU"
                     /locus_tag="VQ516_000693"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2511176.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(27966..28982)
                     /locus_tag="VQ516_000694"
     CDS             complement(27966..28982)
                     /locus_tag="VQ516_000694"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2511177.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            29194..29571
                     /locus_tag="VQ516_000695"
                     /pseudo
     CDS             29194..29571
                     /locus_tag="VQ516_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29751..30176)
                     /locus_tag="VQ516_000696"
     CDS             complement(29751..30176)
                     /locus_tag="VQ516_000696"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2511178.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(30244..30894)
                     /gene="phoU"
                     /locus_tag="VQ516_000697"
     CDS             complement(30244..30894)
                     /gene="phoU"
                     /locus_tag="VQ516_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2511179.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(30909..31661)
                     /gene="pstB"
                     /locus_tag="VQ516_000698"
     CDS             complement(30909..31661)
                     /gene="pstB"
                     /locus_tag="VQ516_000698"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2511180.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(31663..32478)
                     /gene="pstA"
                     /locus_tag="VQ516_000699"
     CDS             complement(31663..32478)
                     /gene="pstA"
                     /locus_tag="VQ516_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2511181.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(32471..33334)
                     /gene="pstC"
                     /locus_tag="VQ516_000700"
     CDS             complement(32471..33334)
                     /gene="pstC"
                     /locus_tag="VQ516_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2511182.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(33452..34327)
                     /locus_tag="VQ516_000701"
     CDS             complement(33452..34327)
                     /locus_tag="VQ516_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2511183.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(34494..35825)
                     /locus_tag="VQ516_000702"
     CDS             complement(34494..35825)
                     /locus_tag="VQ516_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2511184.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(35826..36533)
                     /locus_tag="VQ516_000703"
     CDS             complement(35826..36533)
                     /locus_tag="VQ516_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2511185.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(36617..37102)
                     /locus_tag="VQ516_000704"
     CDS             complement(36617..37102)
                     /locus_tag="VQ516_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2511186.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            37088..37258
                     /locus_tag="VQ516_000705"
                     /pseudo
     CDS             37088..37258
                     /locus_tag="VQ516_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        10926 a         7068 c         8033 g        11231 t
ORIGIN      
        1 gatgaaactg acgaagtcag taacaatacc tacggtaagg cgacgctgac gtggtttgaa
       61 gagattttcg aagagtatta gcgctaattt ttagggcttt cacctgactt ttaaagactg
      121 gcaaactttt ctgatgattt tcatgaagag cctgcgcttt tatggtaaaa tagtaacaga
      181 ataaaagagg agagaaataa tgaaacgtag tatgtatgct ggtcgtgttc gtgaggaaca
      241 catcggacaa gaaataacct tgaaaggatg ggttggccgt cgtcgtgacc ttggtggttt
      301 gatctttatc gatcttcgtg accgtgaagg aatcatgcag ttggttatca accctgaaaa
      361 agtatctgca gaggttatgg caacagctga aagccttcgt agcgaatttg ttattgaggt
      421 gactggtcag gtcgctgcgc gtgagcaagc caatgataag ttgccaactg gtgcggttga
      481 gttaaacgtg acagctctta ttgtgcttaa cacagctaag acaacaccat ttgagattaa
      541 ggatggcatt gaggcaaatg acgatacacg tttgcgttac cgttaccttg accttcgtcg
      601 tccagaaatg ttggaaaatc ttaaacttcg tgccaaggtg acccactcta tccgcaacta
      661 tttggatgag ttggagttta tcgacgtgga gacaccattc ctttctaagt caacgcctga
      721 aggggcgcgt gattatttag tgccgtctcg tgttaataag gggcattttt acgctcttcc
      781 tcaaagtcca caaatcacga aacagctctt gatgaatgct ggttttgacc gttactacca
      841 aatcgttaaa tgtttccgtg acgaggactt gcgtggagac cgccagcctg aatttactca
      901 ggtcgacttg gaaacgtcct tccttactga gaaagaaatc caagatatta cagaaggctt
      961 gatcgcgcgc gtgatgaagg aaacaaaagg catcgaagta acgctaccgt tccctcgtat
     1021 gaaatacgat gatgctatgg ctctttacgg ttctgacaag ccagatactc gttttgacat
     1081 gttgcttcag gacttgacag aagtggtcaa aggtgtagac tttaaagtct tttcagaagc
     1141 acctgctgta aaagcgattg tggtcaaagg agctgcggac aactattcac gtaaagacat
     1201 cgacaagatg acggaagtag ccaaacagta tggtgccaaa ggtcttgctt gggtcaaggt
     1261 ggttgatgga gaattaaacg gaccagttgc caagttcttg actggtatcc aagaagaatt
     1321 gacaacagcg cttgctcttg aagataagga cttggttctc tttgtggcgg atacgcttga
     1381 agtggttaat gcaacactgg gtacccttcg tggacgtatt gctaaagagc ttggcttgat
     1441 tgataatgat aagttcaact tcctttgggt ggttgactgg ccgatgtttg aatggtctga
     1501 agaagaaggc cgctacatga gcgcccacca tccattcaca cttccacagg aagagactgc
     1561 tcacgaatta gaaggtgatt tggctaaggt tcgtgccatt gcttacgata tcgtcttgaa
     1621 cggttatgag cttggtggtg gtagccttcg tatcaaccaa aaagaccttc aagaacgcat
     1681 gttcaaggct cttggtttct caactgaaga agccaatgac cagtttggtt tccttcttga
     1741 agccatggac tatggtttcc caccgcacgg tggtttggct atcgggcttg accgttttgt
     1801 catgttgctt gctggagaag aaaatatccg tgaagtcatt gcctttccta agaacaacaa
     1861 ggcaactgac ccaatgacac aagctccatc aacagtcgct ctcaaacaac tagaggaact
     1921 cagcttacaa gtagaagaag atgaaacaaa caaaacgaat taagcggtgg cgctattatc
     1981 tgcgccgctt tgcttatcag ataaaaattt tacgtgtctt acaaagtatc tctcgagaaa
     2041 agtatgatga gaagatttcg gcctctctgg tctatggttt tttatcagca gtagcagtta
     2101 atttcttttt ccaaccaggg catgtgtatt cgagtggtgc aacaggtctg gcacagatta
     2161 tctctgcctt gagtaatcac tggtttggtt ttcatattcc gatttcgcta agcttctacg
     2221 ccattaactt ccctttgatg gtcttagctt ggtatcagat tggccataag ttcaccgtct
     2281 ttacctttat cacggtatct atgagttcct tctttatcca gtttgtccct gtggcaacct
     2341 tgacggagga tcccattatc aattcccttt ttgggggtgt tgttatgggt ttggggattg
     2401 gttttgctct tcgaaacaat atctccagtg gtgggacgga tatcgtcagc ctgactattc
     2461 gtaagaaaac gggtaagaat gtcggtagta tttctttctt ggtaaatgga actatcatgc
     2521 tgatagcagg tttgaccttt ggttggaaat acgctcttta ttctatgatt accatctttg
     2581 tctctagccg tgtgacagac gcagtcttta ctaagcaaaa gcgtatgcag gccatgattg
     2641 tgacaaatca tccagagaag gtaattgaaa aaatccataa aaaattgcac cgcggagcaa
     2701 ccatgatcca cgatgcagaa ggaacctata atcacgagag aaaggcagtt ttaatcactg
     2761 tcattacacg tgcagagttt aatgaattta aacagattat gacacaagtg gatccaagct
     2821 cctttgtctc tgtctcggaa aatgttcata ttctaggaag atttgttgag atagataatt
     2881 agtgattaat actcttcgaa aatctcttca aaccacgtca gcttcacctt gccgtatata
     2941 tgttactgac ttcgtcagtt ctatctgcaa cctcaaaacc atgttttgag ctgacttcgt
     3001 cagtttcatc tacaacctca aaaccatgtt ttgagcaacc tgcggctagc ttcctagttt
     3061 gcactttgat tttcattgag tataaaaaga tcttgggaaa agactcaatt tcagtagaaa
     3121 aggaagtaaa tcttcccaca ttaaaacgca tagtatcaag ttttttcaat acctgatagt
     3181 atgcgttttt gcttttaacg actttttgcc cagtctctta tttttcgata attttgtggg
     3241 cgatcaattg acggtaacaa atttgtttat tgtttttatt atcattaata atctggagaa
     3301 tagtttcaag ggaaacacga ccaagctcta aactattgat atcgacatag gctgccaagt
     3361 tgagcttggg attaaccgag tcaaagctga gaacagggac atccagctgg tgtttggcaa
     3421 tatagttaca aacgccttca gctaggaggc tatcggttgt gatgatagca tcaatttgtg
     3481 gatcgtgctt gaataatcgc ttgctaaatt tatagccctt ttcttccaga aactcgtcgg
     3541 caaagtagat gcgattgttg tcagtggtaa gtttgtaatg tttaagcgcc tgttcatagc
     3601 ctgttaaacg gtctttggtc acgaagagct ttttacttcc tccgataaag gcaatgcgtt
     3661 tgcagccttt tttgatgaaa tattcagtcg catcaaaacc agcttgaaca ttgtcgttgt
     3721 cgacaagtgg gatgaaagga gatagagatt tacctaagat aaggaagggg aactgttctt
     3781 ctgcgacgag ttttacgaga gggtcttctt cttgggcata gagaaagatt agcccatcta
     3841 cacgcttgcc gtagaccatt tgtgaaatag cgttgagacg ctccttctca tctttccctg
     3901 ttgctatctg aatggcatag tggttttcag atgcgacttg agagatgcca cgtagaaccg
     3961 atggaaagaa aggattctgg tagaaggcgt ctgagtcatc aggaagaact aatccgataa
     4021 cctgagtata gctgcttacc aagctacgag cgttgaggtt tgggtggtag ttgagttcct
     4081 tcatagcttt gcgaacgcgt ttttttgttt cgtcgctaat ggttgattta ttttgaataa
     4141 cacgggttac ggttgaaggc gaaacaccag cagccttggc cacgtcttta atcgtaacgg
     4201 gcataaaaat ctcctattta tggtaatttg gatcacggaa atgtgtttta taaaagatag
     4261 tgaccagata cagaacaaaa agaatatttt gtacagtaat cagggttgtc atattttggc
     4321 caaataatcc caaaataagt gtaatcagag tcggcaatcc taaacagttc aagataaagt
     4381 gatagcactc tttaaaggtt ctaaatgaaa agaggcgtga tttcttagtg atataaagga
     4441 gaagactagc tcctagagag acgataaaga aattcaaacc aaagaggaag ctcgcaccga
     4501 gaactaggaa gaggctgata tagatacgat tttgttggta ccagtcttta gaaattgctt
     4561 gggtcaagct gtctttgctt ttgaaactct cagtctgaat ggctcggtaa gagatgcggg
     4621 tcagttcctt gctttccttg ctgatgacta gttcatttgt atcgaaatgc agttgcaagt
     4681 ccttaggtaa ttccttgatt tgacttggac caatcacaac agaaggggct tgactagctg
     4741 ttccagtata agttaatgtg ccatcgacaa ttgtagcatg ttcagagaga tcctggacaa
     4801 cctcatttgt cagaggttca tagacattat cgataaaagt ttctagcgga taggtctcct
     4861 gggagctgtt ttggatagca atgggtacca tagacaagct gataaggaag atactggtaa
     4921 agagaagttg aaaccagttg agctcgaaac gtttggacag gggcttacga aacccccaaa
     4981 tacttgaaaa ataggaaaat ggatatggaa gcataggtat ctttctaaaa ggtgttttct
     5041 atatgagtat tattatatag agaaatgcgc tttatttcaa gtctaggaga aaaattgctt
     5101 gctacaagga tattatttac agtgacaatt gctaataatc aatgaaaatc aaagtgcaaa
     5161 ccaggaagct agctgcaggt tgctcaaagc acaactttga ggttggagat aaagctgacg
     5221 tggtttgaag agattttcga agagtataaa atgaaaaagg gtggcgggga taaattatcc
     5281 cttgtcgcca ccacttgtaa gtcctgaaac aaagttcttt tgtagaaaga agaagagaat
     5341 acagattgga agggcgatga ggatagcacc tgctgagaag taggcaatct tcaagttttt
     5401 cgcattgtta acgaaggttt ggagacctac ggcaacagta aagtattctt tctcacgaag
     5461 caagaaacta gagaggatgt agtccccgaa aggtcccatg aaggcccaga gagcttgtac
     5521 ggcaaccatt gggcgaacaa gtggtagaac aatttgccag aagcggcgga agtgtcctgc
     5581 accgtctagt tttgcagatt cgtctaaaga cattggcact gtatcgaagt agcctttcat
     5641 gagccaagca ttcatcggga taccaccacc aacgtagagg aagatgagga accagttgtg
     5701 gttaagggcg ttcaacataa gcgccataac gaagaaggct gtcaaagcgg ccatagttgg
     5761 caccatttgg atgatcaaga agaagaccaa actttgttta cgagccaaga agttgtaacg
     5821 gctgtaagca taaccagcaa gtacgatgat acttgtttga acagccatgg taattaaggc
     5881 gataatcaaa gtgttgaggt accaagtacc gtacaaggtt tcagtgaaga ggcctttaaa
     5941 gttatcaaaa ttgaggtcga tattagtatc tagtttaaag gctgagacgt tacctgcttt
     6001 aaaggctgac ataatggtaa tcaacagtgg atagataatt acaattgata gaccaatcag
     6061 gtaaaggtaa gtaaggcttt gagtcagtct acgtttgagt ttaattgagt tattcatctt
     6121 agacgtcctc catatcaaat gcgtgtagtt tcttgaatgc gatcatagag attgagatga
     6181 caatgataga gataatcaag gtaacagctg ccgccattga gtattgagga gatgtacctg
     6241 ttgtcaaacg gtagatccat gagatcaaga tatcggttga accagctcca cctccgacac
     6301 taccaggtcc tccaccattg aagaggtaca tgatagagaa gttgttaaag ttgaaggtgt
     6361 attggctaat caaagtaggt gccgcaacag ccaaaatcat tgggaaagtg atgttgcgga
     6421 atttttgcca agcgttggca ccgtcaatat aagctgcttc gtaaaggtcg ttaggaatag
     6481 attgcaagat acccaaagtc aaaacgtaga tgtatgggaa tccgagccaa ccttgcatca
     6541 taatcaaggc aatcttagtc caagttgggt ctgttttcca aggaataaga gctccatcaa
     6601 ggaaaggaag gaatttagcc aagattggca atacttgagt gttgatagca ccgacactat
     6661 cgttaaacat gtttgagaat gtcaagatag tgatgaaggc tgggacagcc caaggaagaa
     6721 ggaaaataac accaaagata cgttttcctt tgataaatgg ttggttggca atgatagctg
     6781 tgaagatacc aattacgatt tgtaaagtag aagctgccaa agcccaaatg atagtccaag
     6841 aaagaacaga accaaaggca gaacggaagg tactcaagct ccaaatgttt gtaaagttgg
     6901 tcaaaccaac ccagtccaac aacttgtttg gtggcaagtg ttggaagtcg tagttggtaa
     6961 aggcgatcat caaggttacg ataactggga agataatcgc gaaggtcatg gcaacataag
     7021 atggaatgat caagaggtaa gggaagccat tttcataaat ccctttgatc atgtctttga
     7081 gtgtgcgtgg aactggaatt ccattgttaa tgcgttttgc aatcgtatgt gcatctttga
     7141 tatttgagaa ataaaagagt acataaacga ttacaaagat tagatggaag gcaccacgaa
     7201 tcagcataaa gagggaatta tcacgacctg gtttgttacc aagagtgatg aggttgctca
     7261 attcaggggt tgcaagtgct aggaagtaaa ggacaaatac gatggttaca ccaaggaaga
     7321 taaaaccttt ggctttttgt ttattgtaaa tctgtcctaa cccaggaatg atagacagca
     7381 gggctgcttt actaggttgt tgcttttcca tattaactcc tttcatagga tactactttt
     7441 ttataagaaa gctaaattat ttgtgtctct attgataaat tcaaaggggg atttgatttc
     7501 cacccccctt gaacaaattt tttattcacc aaatttttgt ttgattgttt ctttgatcaa
     7561 tgttacagca tcgttagcag ctgttttagc atctttttga ccacttacag catcaaagag
     7621 catatttttc gctggatccc aaactgcaga catttgagag atgtttggca gtggttgagt
     7681 gttcttgaac tgtttgataa cagctgttgt caactcatcg tttttacctt cagcgtatga
     7741 acgagcctca gtattagctg ggatttcgtt agtcttatca tataatactt tttgttgttc
     7801 agttgcaaca aggaagtcta caaatttttg agaagcttca aggttcttaa cggcttgagg
     7861 aatgacccaa gctttaccac caccgaatgc agcatattct tttccatttg gaagagttgg
     7921 gatagttgca actccgtagt ttactttagc atctttaaag gcttgagctt tccaaggtcc
     7981 gtcgatgata gcagctgttt taccttcttg gaattgagtt tggattaagt ttccagcacc
     8041 ttctgtatct tgcatacctt taggccattt ttcgtaccaa gatttagcgt aattgatacc
     8101 tacgatagaa ccgtcgtttg caagaccgat gtctttagcg tctttaccgt tttggccaaa
     8161 gacgtaagca ccgttaccgg caagaagtcc atatgtatag tagaagtttg tccagtcagc
     8221 taggaaggca gtagttttac catcttcacc agcgaatgcg tatttgctat ctttagcaag
     8281 gttttccaag tcagcaaatg tttttggagc atctttcacc aagtctttgt tgtagtacat
     8341 aacaagtgac tcgataacgg caggagcacc gtaaacttta ccattagcag ctgttacaag
     8401 agatttagtt gtgtcgtctg ttttagcacc atcgctcaat ttcacttctg aaagttgtcc
     8461 gtcagaacca aggctaccta cacggtcgta tggagccatc ataacatcag ggacattacc
     8521 agattggttg tcaagagaaa gtttatcaag acctcctaga gcatcaccag ttttaagagt
     8581 gacttttact ccagcttctt tttcataagc tttagcaacc tcttcaatat agctcttata
     8641 tccctcgtct acatatacag tgagttcttt gacttcagat gaaccagaat cagcaggctt
     8701 atcagcagtt ttgcttccgc aagctaccaa aagcaagcta gcaagtgtag cagttccaag
     8761 caccgcagtg ctcttcataa atttagatga catagtgtat tcctcctaaa gaatagcaag
     8821 ttttattgat aaggaaacgc aaacgttttc ctttatgagc ttagtatagc acaaatagaa
     8881 aacggttgca agtatttttt gtaaaaattt taaaaaattt ttaagcttga tttgatagca
     8941 taattttgca ttttaataga agaaaagtat gaaatagata agaatacagt atttttaata
     9001 aaaatatatg gaatctgtct aaagagggaa atcaggaggg gctacctccc tgtttctaat
     9061 ctatatctat tttctataga cgtttgtgct ttgttacata taaattcaag ctccctaagg
     9121 aggttttata ctttgtagac tgtctcgtga gagtcaaacc ttaggattag atagttttaa
     9181 gttatctttg ctaaaatgag ttagtttcct taaaaataat caaaaaagtt ttttaaaaac
     9241 gcttgcaatt atgcttgaaa aggagtatac ttataagtaa cgcaaacgtt tgcgtctgca
     9301 aaatacgcaa cgttccatta ttttaacaca cgaggtgcta ttatgaaaaa acgtcaaagt
     9361 ggtgtgttga tgcacatctc ttctcttcca ggagcttacg gaatcggatc atttggtcaa
     9421 agtgcttacg acttcgttga tttcttggtc cgtacaaaac aacgttactg gcaaatcctt
     9481 ccattaggag caactagtta cggggattct ccttaccaat ctttctcagc cttcgcagga
     9541 aacactcatt ttatcgattt agatatcttg gtggagcaag gtttgttgga agcaagtgac
     9601 cttgaaggag ttgactttgg tagcgatgcg tctgaagttg actatgctaa aatctactat
     9661 gcacgtcgtc ctcttttaga aaaagcggtg aaacgtttct ttgaagtcgg agatgttaaa
     9721 gattttgaga aatttgctca agacaaccaa tcatggcttg agctctttgc tgagtatatg
     9781 gctatcaaag agtattttga caatcttgct tggactgaat ggccagatgc agatgctcgt
     9841 gctcgtaaag cttcagcact tgaaagctat cgtgagcaat tggcagacaa gttggtttac
     9901 caccgtgtga ctcaatactt cttcttccaa caatggttga aattgaaagc ttacgctaac
     9961 gacaaccaca tcgaaatcgt tggggacatg ccaatctacg tagcggaaga ttcaagtgat
    10021 atgtgggcaa atccacatct cttcaaaaca gatgtcaatg gtaaggctac ttgtatcgca
    10081 ggatgcccac cagatgagtt ttctgtaact ggtcagcttt ggggtaaccc aatctatgac
    10141 tgggaagcaa tggacaaaga cggctacaaa tggtggattg aacgcttgcg tgaaagcttc
    10201 aaaatctacg atatcgttcg tatcgaccac ttccgtggct tcgaatctta ctgggaaatc
    10261 cctgctggtt ccgatacagc agcacctggt gagtgggtga aaggtccagg ctacaagctt
    10321 tttgcagccg ttaaggaaga acttggtgag ctaaacatca tcgcagaaga ccttggcttc
    10381 atgacagatg aagtgatcga attgcgtgaa cgtactggct tcccaggaat gaagattctt
    10441 caatttgcct tcaacccaga agacgaaagc attgatagcc cacacttggc acctgctaac
    10501 tcagttatgt acacaggaac acacgataac aatacggttc ttggttggta ccgtaatgag
    10561 attgatgatg cgactcgtga gtacatggct cgttacacga accgtaaaga atacgaaaca
    10621 gtggtacacg ctatgcttcg tacagtattt tcatcagtta gctttatggc aattgcaact
    10681 atgcaagatt tactagaatt ggatgaggca gctcgtatga acttcccatc tacccttggt
    10741 ggaaactggt cttggcgtat gactgaagat caattgacac cagctgtcga ggaaggtttg
    10801 cttgacttga caacaattta tcgccgaatt aatgaaaatt tggtagattt aaagaaataa
    10861 gacaataatc aggagacaac taaacatgtt atcactacaa gaatttgtac aaaatcgtta
    10921 caataaaacc attgcagaat gtagcaatga agagctttac cttgctcttc ttaactacag
    10981 caagcttgca agcagccaaa aaccagtcaa cactggtaag aaaaaagttt actacatctc
    11041 agctgagttc ttgattggta aactcttgtc aaacaacttg attaaccttg gtctttacga
    11101 cgatgttaaa aaagaacttg cagctgcagg taaagacttg atcgaagttg aagaagttga
    11161 attggaacca tctcttggta atggtggttt gggacgtttg gctgcctgct ttatcgactc
    11221 aattgctact cttggtttga atggtgacgg tgttggtctt aactaccact ttggtctttt
    11281 ccaacaagtt cttaaaaaca accaacaaga aacaattcca aatgcatggt tgacagagca
    11341 aaactggttg gttcgctcaa gccgtagcta ccaagtacca tttgcagact ttactttgac
    11401 atcaactctt tacgatattg atgttactgg ttatgaaaca gcgactaaaa accgcttgcg
    11461 tttgtttgac ttggattcag ttgattcttc tattattaaa gatggtatca actttgacaa
    11521 gacagatatc gctcgcaact taactctctt cctttaccca gatgatagtg accgtcaagg
    11581 tgaattgctc cgtatcttcc aacaatactt catggtttca aacggtgcgc aattgatcat
    11641 cgacgaagca atcgaaaaag gaagcaactt gcatgacctt gctgactacg cagttgtcca
    11701 aatcaacgat actcacccat caatggtgat tcctgaattg attcgtcttt tgactgcacg
    11761 tggtatcgat cttgacgaag caatctcaat tgttcgtagc atgactgcct acactaacca
    11821 cacaatcctt gctgaagcgc ttgaaaaatg gcctcttgaa ttcttgcaag aagtggttcc
    11881 tcacttggta ccaatcatcg aagaattgga ccgtcgtgtg aaggcagagt acaaagatcc
    11941 agctgttcaa atcatcgatg agagcggacg tgttcacatg gctcacatgg atatccacta
    12001 cggatacagt gttaacgggg ttgcagcact ccatactgaa atcttgaaaa attctgagtt
    12061 gaaagccttc tacgaccttt acccagaaaa gttcaacaac aaaacaaacg gtatcacttt
    12121 ccgtcgttgg cttatgcatg ctaacccaag attgtctcac tacttggatg agattcttgg
    12181 agatggttgg caccatgaag cagatgagct tgaaaaactg ttgtcttatg aagacaaagc
    12241 agctgtcaaa gaaaaattgg aaagcatcaa ggctcacaac aaacgtaaat tggctcgtca
    12301 cttgaaagaa caccaaggtg tggaaatcaa tccaaattct atctttgata tccaaatcaa
    12361 acgtcttcac gagtacaaac gccaacaaat gaacgctttg tacgtgatcc acaaatacct
    12421 tgacatcaaa gctggtaaca tccctgctcg tccaatcaca atcttctttg gtggtaaagc
    12481 agctccagcc tacacaatcg ctcaagatat cattcacttg atcctttgca tgtcagaagt
    12541 tattgctaac gatccagcag tagctccaca cttgcaagta gttatggttg aaaactacaa
    12601 cgttactgca gcaagcttcc ttatcccagc atgtgatatc tcagaacaaa tctcacttgc
    12661 ttctaaagaa gcttcaggta ctggtaacat gaaattcatg ttgaacggag ctttgacact
    12721 tggtactatg gacggtgcta acgtggaaat cgctgagttg gttggagaag aaaacatcta
    12781 catcttcggt gaagattcag aaactgttat cgacctttac gcaaaagcag cttacaaatc
    12841 aagcgaattc tacgctcgtg aagctatcaa accattggtt gacttcatcg ttagtgatgc
    12901 agttcttgca gctggaaaca aagagcgctt ggaacgtctt tacaatgaat tgatcaacaa
    12961 agactggttc atgactcttc ttgacttgga agactacatc aaagtcaaag agcaaatgct
    13021 tgctgactac gaagaccgtg acgcatggtt ggataaagtc atcgttaaca tttctaaagc
    13081 aggattcttc tcatctgacc gtacaatcgc tcagtataac gaagacatct ggcacttgaa
    13141 ctaatactct tcgaaaatct cttcaaacca cgtcagcttt atctgcaacc tcaaagcggt
    13201 gctttgagca acctgcggtt agcttcctag tttactcttt gattttcatt gagtataaga
    13261 tacaaattta tactaataca tcttgtaaaa aagcgagttt cgattgaaat tcgctttttt
    13321 aatgatgtag atttgggtca atcttgtcta aaaataggga aatcctagat acagtgaagg
    13381 ctttaaatgc tggtttttac tgtcctcagc cttatatttt ttcgtagttg attacctcat
    13441 acctcttgta ttcgcttaca taaagtatta taatataatt gtaggaaaga aggtgttttt
    13501 atgatataca cacttaaatt ggtgttgttt attacctttc ttgtaataag cttgttacct
    13561 gataagattt ttggaaaaaa taaaaaaatt tggaaaatag tttttgcaat attgacggca
    13621 gtggcagcat tgtcatttat gtactaagtt attttaagaa tgtagggaaa taaaccctac
    13681 attcttttta gttttttctg ttttctaaat tctatttatc caagcgcttc aacatttctt
    13741 gcttcttcgc ttcaagttct gcacgctttt cttcgatttc ggcatgtttt ttctcgagtt
    13801 cagaacaact tgcaccattg ctaaattctt ttcgccatca ggagataggg tgagtcgaca
    13861 tgtctattac tcacccaaag cagtcctaca aagcaggaat tttctgttac ttttttggaa
    13921 atagtaacgt ttatacagct ttgacacttc gtatcaaagc gccaaacaca ctccgaggga
    13981 tttacagaaa gcagaaaagg aatgatctgg tataagatca ttccttttct ctctttttct
    14041 ttaagtaatt atatacaatg tacgacgaag tcgtcattgc aatgctgatc caccacctaa
    14101 aggaaacttt aaacaacatt gataagataa agaatataaa caacgaaaat acgttatacc
    14161 caattaattt tattgtatat ctcatgatta aaagttaatc cttccgttgt taggaatggc
    14221 atcattttta tcccataatt gtgctaaata agtccccggt gataataaat ttatagcaaa
    14281 ttctaaagca acatcattta caaaccaact acctagatat ctagaaattg ctgaacgaat
    14341 agcacttttt gctgcatgtt ttccttttac tttaattaga tttgcaaggc ctgcagtagt
    14401 tcctcctaat gctaaagcta ttgcagtatc taatagagca cccatttgat taactgtaat
    14461 accttgccaa gctgctctaa atggagagta tgtaggtggg attgtataat cgccttgtaa
    14521 ttgtcggtta attacttctt tgatccattg ttgtgagacg tctggatgaa aagattggat
    14581 ttcgtttgca agtgtattga tttgttcttc tgttagagaa gtgacaggtt gaagttccat
    14641 atttgtttca atttgtgata cttgttcaga agcgtataca gctgaaacac ttggaatcgc
    14701 tgatacaatt aacacaattg acgtcaaaaa aaccgaaata aatttcatta atttgttcat
    14761 gagcttttct cctttttatt tgtatctgct tacattttat catatgctgt tattatagtc
    14821 aaaaaaatat gctattatgt taaaaaaata tttttcaaaa tataaatgga cggatttatt
    14881 ttggatttta tttgttattt tgacctgcct ctatattggt aaccatgatt tgtttactct
    14941 caatcatcaa gaattctctt ttcgtggtag cgtttggggt ctggtactgg ccttatatca
    15001 cttactattc attgataagt ttgttatatc gaatcgaaaa taaagattag agctatgctt
    15061 gactgtgtac ttttaggatt gattttggag gaagattttg tctctattat ttattatttt
    15121 aaatttattt attttgtata agatctattc tttgaggcgg gaaaaatcga aatacttgat
    15181 ttatacggcc tatatcatat ttggggtaaa tgtagtatat ggtattcaat ggttattaag
    15241 agaactgatt tcaactattt ccccttaatg tgtatggaaa ctataacaaa acgcataaga
    15301 ttaaggtttg tgtacctgtt cttatgcgtt tttataatat tgaagatttt cggtaagttt
    15361 tatacctttg ggaagtctag tttagaatgc tttcccaacc aaaatctctg cttcgatggt
    15421 aatctctgtc agttggctcc agtcaatctt gctctggtca acgtgaaaga ggagaggagt
    15481 catactgagt agggcttgga gttgttctgc tgtgatagtc ttagtcagag aggcagtttg
    15541 actagatagg atggtaaagt gttcctggaa atgctccttg atatcttggt tagaatactc
    15601 cttgtttgtc agctggtcct gtactctttg acggatttct ttgaggtgat tttcagttgg
    15661 gataaccttt atcaagatac cgtctttgga taaaacgcga cgaaattctc catagttggc
    15721 aggtgagaag atatcaagca gaatatccat gttagcgtct tttataggaa gtcgtgccaa
    15781 gtcgccaacg aaccaattga ctgcccagtt gggttcactt ttagcagcga tttggactga
    15841 atctttggag atgtcaaagg catagaaagt tttttcagag tgactttctt gtagtttgcg
    15901 agaatagaat ccttcaccac aaccgatatc caaaattgtt gtggtagttt ttgagcttgc
    15961 aagcaagtca gatacagcat ctaagatagc ttggtaaaag ccggcttcta ggatttgttg
    16021 acggttttga aaattttcct tgtcatagtt agcagattgc ttgatttgag gtgctagatt
    16081 gacatagcca aatttcgcca agtcaaaaga atgacggttg cagcacttga aattagtctc
    16141 taacagagtc agattttctt gacagatagg acaggcaaag gcagtcgcag aagcaaaacg
    16201 ctgaagtttg ggcttgagat ttgtattcat agtttaagtg tatcaaaaga ggcaaatgaa
    16261 agcaacttta ggaataagta gattcagtag aaataaaact aattctccaa tttatagaat
    16321 gaaattgctt ttatatctaa attgctatat aataatgata aggaggaaat aagtatgtta
    16381 aaagaagtat taaccgttgc aaaagttgcg aaaaaatctt cattattttt gggtggtgtc
    16441 gcatttggta cccttggttt gaaaatctta gcaagtaagg aagctaaaaa aggttattct
    16501 aaagctttgg ctaaggctta caagttgaaa gacgagctag atgcatctgt ttctgttgtg
    16561 aagcaacatg gagacgatgt cttgcaagat gccaaatatt tgtacgagca agagaaaaaa
    16621 gaagagcaat tagatagcct tataggtgaa taatatgtct tttaaagtgc tacatagagg
    16681 ataccaacat atccgactat catcttcttt ttcacttacc ttggatattc aagactatct
    16741 tcgttccttg gcgagagatg aaaaggggat tgagtctatc cagttttaca tggatcaaca
    16801 gcactttact ctacgcataa aagaaggctt ttctgtatta gataatgcag aagccttttt
    16861 aaaaagaatt gataaaggga aagtttctga gttgatgact cttcccattc gtagagaaga
    16921 gagtgcttat tctattgttt caggtgcagc ggttaagcgt gtactttttc gtagttttgt
    16981 gccgtatcct attcgctata tatggacttg ttatcaggct ttgggttata ttagagaagc
    17041 ctatcaaaca ctagcgcgta aggaactaac gatggaagtc ttggactgtt cggctatttt
    17101 attgtccttg tttatgaacc aatccaagac agctagcaat atcatgttta tgcttgattt
    17161 ggggaatcat ttagatcagt ggtccttgaa aaaaactgca acagatttag aacagagtct
    17221 tcttgcaaaa gagagcgatg tattcctagt acagggcgat acggttgtta gtatcaagag
    17281 ttccgatgtt caaataggag atgtcttgat cttatctcaa ggaaatgaaa ttctgtttga
    17341 tggacaagta gtttcaggtt taggtatggt caacgaaagt tccttgacag gagagagttt
    17401 tccagttgaa aaaagagagt ctgatttggt ttgtgcaaat acagtattag aaactggaga
    17461 gttacgcatt cgtgtaacag ataatcagat gaacagccgt attttacagc tgattgagtt
    17521 gatgaagaaa tctgaagaaa acaagaaaac gaaacaacgc tatttcatca agatggcgga
    17581 taaagtcgtc aaatataatt tcttgggggc tgggctgact tacctattga caggttcttt
    17641 ttctaaggct atttctttcc tattggtcga tttctcctgc gctttgaaaa tctctactcc
    17701 tgtagcttat ttgacagtta tcaaggaagg gttgaaccgt gaaatggtga ttaaggatgg
    17761 agatgttctg gagaaatatc tggaagttga tactttcttg tttgataaga caggaacaat
    17821 cacaactagt tatcctatag ttgaaaaggt gttacctttt ggagactata gtgaggaaga
    17881 tattctcaga atcagtgcct gtcttgagga acacatttat catcctattg ctaatgccat
    17941 cgtcaagcaa gctgagatag agggaattga acatgaggaa atgcatggga aactccaata
    18001 tatcgcaagc aaggggatca aatctcatat agatggccaa ccagttctta ttgggaatta
    18061 tgtcttgatg caggatgagc agattcatat cagttcagaa caaaatgctt taattgaaga
    18121 gtacaagagt cactacaatc tcttattctt ggcttatcaa aatgaattga ttggaatgtt
    18181 ctgcattcat actcctttga gaaaagaagc aaaaacagcc ttggataaac ttaaggcaca
    18241 ggggaaaaaa ttgattctgg caacaggaga caccttgatt aggacagagg aattagtcaa
    18301 agatttgccc tttgatcagg tctatacaga cttgaaacct gatgggaaat ttgagttagt
    18361 agagaaactg cagaaagcag gtcacactat tttgatggtt ggagatggat tgaatgactc
    18421 agcggcttta actctatcag atatcggtgt ggtgatgaat gagagtgcag atatttctaa
    18481 gcagatgagt gatatcttat tgttagataa tcgtttggat ttcttccaag agttggattc
    18541 gttatcatca tctttgcaaa cactcatcaa gaaaaatatt caggataccg ttgtcgtaaa
    18601 tagtagtttg attggctttg gcttatttaa ttggttcagt ccttcaaatc tctctatctt
    18661 acataatcta acaaccttgc gcattgtcct gcgtagcctg tctattaaaa atagatagat
    18721 gagagttatt aacagcaaaa aggagttacc tttctcgagg ttaactcctt tgtttataga
    18781 taaatgttga atagtttagt aagtcaatac aaagtatttt ttcttcccac gacggataac
    18841 agtcagttca ttttctaact tatcagcgtc actcaagaca tagtcaaggt cttggatgcg
    18901 gtcgccgttt acgtagatgg ctccgttttg gacgtcttca cgggcttggc gttttgagtt
    18961 aactatacca gatgagacga gcagttccac gatattgttg ttttcgtctg cctgtacttg
    19021 gtagtttggc acaccacgaa gtccttgttt gagctctttg acagaaaggt ttttgatgtt
    19081 tcctgcaaag agttgctcag tgatgttaag tgcttctttg taggcttctt ctccgtgaac
    19141 aagtgtaaca acttcacgag ccaagacttt ttgagccaag cgttcgtgtg gcgctgcttc
    19201 aaattgttta cgaatatctt caatctcatc aagtgacaag aaagtaaaga ttttcaagaa
    19261 gcgaacagcg tcagcgtcca tcacgttcat ccagaattgg tacatttcgt atggagaagt
    19321 cttttcggga ttgagccaga cggcatttcc ttctgattta ccaaattttt taccagttgc
    19381 atctgtgatt agtggaacag tgataacgtg accagtcttg tccgccttac gacgaagcaa
    19441 ttcggtacca gctgtcatat ttccccactg gtcagaacca ccgatttgaa gagtgacatt
    19501 atggtcttgg ttaaggacga agaagtcgta cccttgcatg atttggtaag cgaactcagt
    19561 gtaagaaatt cctgtttcga tccgtttttt aacagattcc ttactcatca tgtagttgac
    19621 cgtgaagtac tttccaatgt cacggaggaa gtcaatgaag ctgatgctgc caaaccagtc
    19681 gtagttgttg accatgacag ccttgttttc gccattttca aagtcaagaa aacgagaaag
    19741 ttgtccttgg atagacttga cccagccatc tactgtgtct tttgtttgga gactacgttc
    19801 agcatctttg aaggacggat ctccgatgag acctgtagca ccgccaacga gcgcataagg
    19861 tttgtgacct gctagttgca agcgacgact tgtcaagatt gcgacaaggt ggcctaggtg
    19921 aaggctgtca gcagttggat cgtagccagt ataataagaa acttgacctt cttctagggc
    19981 tttacgcaaa gcttcttcat cagtcgtttg aaatatcaaa ccacgctctt ttagctcatc
    20041 aaaaatgtgc atgtgtcttt tctccttttt aaaattattg tttctaccta ttgtatcaca
    20101 aacttgggca atagcctagc agaataggga agaaagtggc tattttattg aaaatttccc
    20161 ttttgtggta taatagataa agtgaggaaa tccatgcaaa atcaattaaa tgaattaaaa
    20221 cgaaaaatgc tggaattttt ccagcaaaaa caaaaaaata aaaaatcagc tagacctggc
    20281 aagaaaggtt caagtaccaa aaaatctaaa accttagata agtcagccat tttcccagct
    20341 attttactga gtataaaagc cttatttaac ttactctttg tactcggttt tctaggagga
    20401 atgttgggag ttgggattgc tttggggtac ggagtggcct tatttgacaa ggttcgggtg
    20461 cctcagacag aagaattggt gaatcaggtc aaggacatct cttctatttc agagattacc
    20521 tattcggacg ggacggtgat tgcttccata gagagtgatt tgttgcgcac ttctatctca
    20581 tctgagcaaa tttcggaaaa tctgaagaag gctatcattg cgacagaaga tgaacacttt
    20641 aaagaacata agggtgtagt acccaaggcg gtgattcgtg cgaccttggg gaaatttgta
    20701 ggtttgggtt cctctagtgg gggttcaacc ttgacccagc aactaattaa acagcaggtt
    20761 gttggggatg cgccgacctt ggctcgtaag gcggcagaga ttgtggatgc tcttgccttg
    20821 gaacgcgcca tgaataaaga tgagatttta acgacctatc tcaatgtggc tccctttggc
    20881 cgaaataata agggacagaa tattgcaggg gctcggcaag cagctgaggg aattttcggt
    20941 gtagatgcca gtcagttgac tgttcctcaa gcagcatttt tagcaggact tccacagagt
    21001 cccattactt actctcctta tgaaaatact ggggagttga agagtgatga agacctagaa
    21061 attggcttaa gacgggctaa ggcagttctt tacagtatgt atcgtacagg tgcattaagc
    21121 aaagacgagt attctcagta caaggattat gaccttaaac aggacttttt accatcgggc
    21181 acggttacag gaatttcacg agactattta tactttacaa ctttggcaga agctcaagaa
    21241 cgtatgtatg actatctagc tcagagagac aatgtctccg ctaaggagtt gaaaaatgag
    21301 gcaactcaga agttttatcg agatttggca gccaaggaaa ttgaaaatgg tggttataag
    21361 attactacta ccatagatca gaaaattcat tctgccatgc aaagtgcggt tgctgattat
    21421 ggctatcttt tagacgatgg aacaggtcgt gtagaagtag ggaatgtctt gatggataac
    21481 caaacaggtg ctattctagg ctttgtaggt ggtcgtaatt atcaagaaaa tcaaaataat
    21541 catgcctttg atacaaaacg ttcgccagct tctactacca agcccttgct ggcctacggt
    21601 attgctattg accagggctt gatgggaagt gaaacgattc tatctaacta tccaacaaac
    21661 tttgctaatg gcaatccgat tatgtatgct aatagcaagg gaacaggaat gatgaccttg
    21721 ggagaagctc tgaactattc atggaatatc cctgcttact ggacctatcg tatgctccgt
    21781 gaaaagggtg ttgatgtcaa gggttatatg gaaaagatgg gttacgagat tcctgagtac
    21841 ggtattgaga gcttgccaat gggtggtggt attgaagtca cagttgccca gcataccaat
    21901 ggctatcaga ccttagctaa taatggagtt tatcatcaga agcatgtgat ttcaaagatt
    21961 gaagcagcag atggtagagt ggtgtatgag tatcaggata aaccggttca agtctattca
    22021 aaagctactg cgacgattat gcagggattg ctacgagaag ttctatcctc tcgtgtgaca
    22081 acaaccttca agtctaacct gacttcttta aatcctactc tggctaatgc agattggatt
    22141 gggaagactg gtacaaccaa ccaagacgaa aatatgtggc tcatgctttc gacacctaga
    22201 ttaaccctag gtggctggat tgggcatgat gataatcatt cattgtcacg tagagcaggt
    22261 tattctaata actctaatta catggctcat ctggtaaatg cgattcagca agcttcccca
    22321 agcatttggg ggaacgagcg ctttgcttta gatcctagtg tagtgaaatc ggaagtcttg
    22381 aaatcaacag gtcaaaaacc agggaaggtt tctgttgaag gaaaagaggt agaggtcaca
    22441 ggttcgactg ttaccagcta ttgggctaat aagtcaggag cgccagcgac aagttatcgc
    22501 tttgctattg gcggaagtga tgcggattat cagaatgctt ggtctagtat tgtggggagt
    22561 ctaccaactc catccagctc cagcagttca agtagtagtt ctagcgatag cagtaactca
    22621 agtactacac gaccttcttc ttcaagggcg agacgataat tctctaaatg aagtggccaa
    22681 tcaatggatt gccacttttt tcctttcgtg ttacaataag ggtatgaatc agtatcagaa
    22741 aaagattgtt aacggaaaaa tttattcgct cctatccggc ttaatatggg gaatctgtgg
    22801 aattttagga gagtacttct ttactcatta tcaggtgtct tcgggctgga ttacctctat
    22861 gcgtttgaca ctggcaggga gtcttgtact catttggtct gcaatacaat taaaatcgca
    22921 agtgctagat atttggcgag acaagaaaaa ttacctgccc tttttagcct atgctatttt
    22981 ggggattttt tcagttcagt attttttcta tctctgttta gaatactcaa atgctacgac
    23041 agcaactatt ttacagttta ttagccctgt ctttatcctc ttttacaatc gcttggttta
    23101 tcaaaaacga gcgtcaaaaa gcgctgtttt ctatgttttg gttgccatgc tgggtgtttg
    23161 cttgatggcg acaaagggag atctctctca gttatccatg acgccgctag ctcttataac
    23221 aggtttgctg agtgccatgg gtgttatgtt taatgttatc ttgccccaac cttttgctaa
    23281 gcgttatggt tttgttccta cggttgggtg ggggatgatt ttggcaggtt tgtttagcaa
    23341 tgtcctctcg ccggtttatc agctttcctt tactcttgat atttggagta tcttgatttg
    23401 cctcattatc gctttctttg gaacggcttt tgcttttttc atttccatga aggctgtgtc
    23461 cttggtttct cctttggtgg tttccgttat cagtgccagt gaacctctct cttctgctct
    23521 cttgagtgtt ttgttcttag gattagtagt ggattggtcc ctccttctag ctatagcctt
    23581 gattatttta cccatgatat ttttgtctat agaagaagcg aaagaaagta gataaaaagt
    23641 cttttcttaa ttctaaaagt gtgctatact agaatagtca ataaaacacg gggagatagt
    23701 tgtgaagagt atttttaggt tgtatcggta ggggcttgct tttaccgaca gcacgtttta
    23761 tctcacatcc ctaaaaaatc atacctaaaa acagacaaaa aggtttcaaa tttgaggcct
    23821 tttttggtag aataggtatc attataacga accaggaggc acctatgact gctacaaaaa
    23881 tgaacgctca agaaattatc caatttatcg ccaatgctga aaagaaaacc agtgtcaaag
    23941 taacctttga ggggcaactc gcaactgctg tgcctagctc tgttgtcaaa ctaggaaatg
    24001 tcctattcgg agactggaag gacgtggctc cgcttcttga aggtttggta gaaaatcaag
    24061 attatgttgt cgagcaagat gctcgtaatt ctgcagttcc tttgctagat aagcgtgcta
    24121 tcaacgctcg tatcgagcca ggtgctatta tccgtgacca ggtggaaatt ggtgacaatg
    24181 ctgttatcat gatgggagct gttatcaata tcggtgctga aatcggtgct ggaaccatga
    24241 ttgacatggg tgccatcctt ggtggccgtg ctatcgttgg aaaaaatagc cacgttggtg
    24301 caggtgcagt tttggcaggt gtgattgagc cagctagtgc tgaaccagtc tgtgtcggag
    24361 acaatgttct tatcggggct aatgcagtgg ttatcgaagg agtccaaatc ggtagtggtt
    24421 cagttgtcgc tgcaggagct atcgttaccc aagatgtccc agaaaacgtg gtagtagcag
    24481 gtgttccagc tcgtattatc aaagaaattg atgcccaaac tcaacaaaaa acagcgctag
    24541 aggatgcgct tcgtaccttg taattgtaaa agtaaaaaag aggcggaacc ctttttccag
    24601 cctctttctg ctatatcgga ggacagatag atgttagatt tgattcagac tagacgagct
    24661 ttacaccaga ttccagagat tggcttggag gagttcaaga ctcaggctta tttgctggat
    24721 gtgattgaga aattgactac gggcaaggat tttgttcaaa ttcgtacttg gcggacaggg
    24781 attttggtct acctgcaggg aagtcagccg gagcgaacca ttggttggcg gacagatatt
    24841 gatggcctgc ctatcgtcga acaaacaggt ctacctttta cttctcaaca ccaaggtcgc
    24901 atgcatgcct gtggtcatga ttttcatatg accattgcct taggctgtct tgagcgcgcc
    24961 cttgaggagc aacccaagaa taatctgctc tttctatttc agcctgctga agaaaatgaa
    25021 gctggtggta tgctcatgta tgaggacggt gcttttggag attggttgcc aaaccaattc
    25081 tatggtctcc atgttcgtcc agatttgaaa gtcggccaga ttgcgactaa tactcataca
    25141 ctctttgcag gaacttgcga ggtgaagatc cgtttcaaag gtaaaggtgg ccacgcagct
    25201 tttcctcatg aagccaatga cgccttggtg gcggctagtt actttgtgac ccaagtgcag
    25261 tcagttgtta gccgcaatgt caacccaatc gagggagcag tggtgacctt cggcgttttt
    25321 caagctggaa ccaccaacaa tgtcatcaca gacacagctt ttttacatgg aactattcgt
    25381 gccttgacac aggacatgag tctcttggtg caaaagaggg taaaaacagt tgcagaaggg
    25441 gttgcagctg cctttgatat ggaagtcgaa gtggaactca aacaaggtgg atacctacct
    25501 gttgagaaca atccagcctt ggcgcgtgaa ctgatggact tctttgacga gaaagacgga
    25561 atcgagttga ttgatatcga acctgctatg acaggcgagg actttggtta tctcctttca
    25621 aaggttgatg gcgtgatgtt ttggctaggt atcgatagtc cctacgctct tcatcaccct
    25681 caaatgagtc ctaaggaaga agtcttagct attggggtgg ctgcggtgtc tagtttcctg
    25741 aaaaagaagg cagcagaata gagggcttgt ctatgaaatc ggaattacgc aagcaagtct
    25801 tgcatgaaat gaaggctata tctcaagagc aaaaacaggc tatagaccaa gctttaaccg
    25861 agcgaatttt acaacacccc ttttatcaag aagccaaggt catcgcaacc tacctctctt
    25921 tttctcatga gtttcaaacg cgggaactga ttgagcaggc gctgaaggac ggcaagaagg
    25981 ttttgacacc taagacctat cccaaggggc gcatggattt tgtggtctat gatccgcaac
    26041 agttggtaaa aacttccttt gccttactgg agccacaggg agatttggaa gtagtggatg
    26101 catctcagat tgatttgatt catgttcctg gcctggcttt tacgacggat ggatatcgga
    26161 ttggatatgg tggaggttat tatgaccgct atctggaaca tttttctggt cgtgctttga
    26221 gtacgatata tccttgtcaa attcaggact ttatacctga aaaccatgat attcctgttc
    26281 aggaggtatt aattgatgaa ggaaatcttt gatagacgtt accctgtgac gagtttcttc
    26341 ctcttagtga cggccttggt atttttacta atgttggtca ctgcaggcgg aaactttgac
    26401 agggcagata cattatttcg atttggagcc atgtatgggc cagctattcg cctctttccc
    26461 gagcaggttt ggcgtctctt gtctgccatt tttgttcata ttgggtggga acatttcatt
    26521 gttaatatgc tttcacttta ttatcttgga aggcaggtag aggagatttt cggttctaag
    26581 cagtttttct ttctctatct tttatcagga atgatgggca atctctttgt ttttgtattt
    26641 agtcctaaat ccttagcagc aggcgcctct acctctcttt atgggctatt tgccgcgatt
    26701 attgttcttc gctatgcaac tcgcaatcct tatattcaac agctagggca atcctatctg
    26761 acactttttg tagttaacat tattggaagt gttctgattc caggaatcag cctagcaggc
    26821 catatcggtg gtgcagttgg tggcgcattt ctagcagtta tctttccagt tagaggagaa
    26881 aaacggatgt ataataccag ccagagatta ggagcggtag tcttgttcgt aggactcgcc
    26941 attttgcttt tctacaaggg aatgggaatg tgacgaagtt gaaagaaaat ttgtgtaatg
    27001 aaaaaagata aggcattttt tgaaccttat ctttttattt tattttattc cttctcagtc
    27061 aattcttttc caagttggat gaggtaattc ttcaaatcat ctttgacttg tgggtgtttg
    27121 agggcgtagt cgatggatgt tttcatgaag ccaaacttgt ctccgacatc gtaacgagcc
    27181 ccattgaact cacgagcaaa tacacgttgt gttttattga gggtgtcgat tgcatctgtc
    27241 agctgaattt catttcctgc acctggagct tggttttcga ggatttggaa aatttcaggc
    27301 gtgaggaggt agcgtccgat aatagcaagg tcgctaggag cgtcctctgg agctggtttt
    27361 tcaacaaagg tttcaacact gtaaagacca tcttttcctt cgccttgcgg agcaataacc
    27421 ccgtaagcag atacttcgtc atgagggact ggcatgacag cgatagtaga cgcgtgggta
    27481 cgctcgtagt catccatgag ttgtttggta agtggaacag ccttttcgtc tgtgatatcc
    27541 atcaagtcat caccaagcat aacgacaaaa ggttcatttc cgacgaaagc cttagcttgc
    27601 aaaacagcat ctccgagacc gcgtggatga gtttggcgga taaaatgcag acgcatgtca
    27661 gttgttttat caactagctt caaaagatct gttttccctt tttctttgag gttatattcc
    27721 aattcgaagt ttgaatcaaa gtggtcctca atagaacgtt ttgatttacc agtgacaact
    27781 agaatatctt caatacctga tttgagagcc tcttccacga taaactggat agttggtttg
    27841 tctacgattg gcaacatttc tttggcaagg gccttggttg ctggtaaaaa tcgagttcct
    27901 agtccagcag cagggatgac tgcctttcta acttttgatg tcataaaaat cctttctata
    27961 gagggttaag accactcatt ttctgcttta aattcattgt tcatgatgtc ataaatggca
    28021 tctttgatat tggttccgtg ataaataact tggtaaatag cctgtgtaat gggcatatag
    28081 actccaagtt cttgggctag ttcataggct gctcgagtcg ttgaaattcc ttcgattacc
    28141 atgcccatat tagcttctat atcagctagg gattctcctc gtccgagagc atctccagct
    28201 ctccagttac gagagtggat ggaagttccc gttacgatca aatctcccac accagataag
    28261 ccgctatagg tcaatggact ggccccgagt gctaccccta ggcgggtgat ttctgctaaa
    28321 cctcgagcga tgatggctgc cttagcatta tcaccaaatc caagaccatg taaagctcca
    28381 gcaccgacag caataatatt tttaagagca ccagcagttt caaccccgat aacatccgta
    28441 ttggtataaa gtcggaagta gtgattacta aatagctcct gaacgtattg agctgtttgt
    28501 aaatctttag aagcagcagt gattaaagtt aggtcacgca caatggtttc ttccgcatga
    28561 ctaggccctg aaacaacgac aatatcactg cggagctgtt caggaatttc ttcttcaagg
    28621 atagttgata atcgtttatg gctatcaggt tctaatccct ttgaagcgtg catgatgata
    28681 gccttatggt ccaaggtttg tgcaacttgt tgggcaacaa gtcgtgtcac ttttgttggg
    28741 acaacaaaca aaatcgcatc cacatctttc aatgtttctg ctaagtcggt gtaggcaatg
    28801 atattttcat ctagaacgac atctttaaag tagtgcttgt tagtatgata ggtattaatt
    28861 tcattgattt gctcgggaag atttccccaa atacgtacct cgtgtccatt gtcatttaag
    28921 acttgtgaaa gggcagttcc ccaagaacca ggccccaaga cggcgacggt ttgtttttcc
    28981 atgatttcct ccttgataga gttctttcca ttattctatc atattctaga ggattttgca
    29041 cttgcattac tggggtatgg tggaaaaata cacaaagacc gagatgaagg gataaactct
    29101 tagtctttag ttaaagtgag aagaaatctg atagatagtt tgattcagta tatgctataa
    29161 atagaggtaa acaactcagg aggttcttgt aggttgcgag agtttggcga aaaaattaaa
    29221 agattacgtt tggctaaaaa aatcagtcgt tcagaatttt gtggtgatga gtctgaatta
    29281 agtatccgtc aattaattag aattgaaaat ggagaatcca gaccaacact aacaaagtta
    29341 aaatatatcg ctgaacgttt ggggggggtg aagattacaa gttgatgcca agttatatag
    29401 agttggataa ggaataccta gaattgaagt atttcttgat gaggactcct acatacgaag
    29461 atgaaactat cgcccaaaag aaagagagtg tttttgctaa gatttttgaa gagtattatg
    29521 ataggctacc tgaggaagaa agatttatca tcccaaatta ttcatatcta gcactagcga
    29581 actacacagt tcaaaaatta ccagaaaagc tagttgaaat actgtccttc tggtgatttt
    29641 gcttaatttc tgatgttcga gacatgattg aggagatttg tcttgatttt ttcctaaaaa
    29701 agggcagact cctcccttgg tttgatgctc gatttttaaa ataaggtgac ttattctgtt
    29761 aggcatgttt tcccctgccc tctagctcta tatggaacag ggagtatcag atttatctgg
    29821 cagattgtat caaataaggc ttgaaactgt tttgaatagg cgtatagact tgagaatttg
    29881 agaatatgtc gtctagcagt agagacatat tttccggcaa tatgaaggaa gagacgtcga
    29941 aaacgcttga tagtcaagga ctttacttca tcaccagcta gctgctttaa aaagaggtag
    30001 agattataag ccagacagcc catcatcata cgtacttcat tcttaatcat ggtcgaacta
    30061 tctgtcttat ccccaaagaa tcctgctttc ctttctttga taaagttttc agcattccct
    30121 cgttcacgat agaattgaaa cagctcctca ctatctacac tgcttaaatt gttcaaatga
    30181 acttttttac aaccataaaa aagagcactt aatcgtactc ttctctttta aggattagtt
    30241 gaattaattc aaatccacta gttctcctgt ttctaggtag actaggcgtt cacaaatgtt
    30301 agcaatgtaa tcagcgaagc gttccagatg tcctatgata taaaggtatt gagttccatt
    30361 gggaattgag gtttcttggt ctttcataag tccaatgatt tcctttgata aggcataata
    30421 atattggtca atctgttcat ctttttgagc aatactaata gcttttgagg cttggtgcaa
    30481 aggaaaggca accaataaat cggctagcat gctgagggat aatttaccca tttggtgtaa
    30541 ctgttcttcg tcaggggcta gttgattttc ttttagttgc aaaacagctt tggcaatgcc
    30601 tgccatatgg tctcccatac gttcaaggtc tgaacaagaa gacatgatgc taatcacaaa
    30661 tcgaaggtca gacacttgtg gctgttgcaa ggccaacaaa cgggcacagg tcaattcgat
    30721 agcgctttga ccttggttga tagcatgatc cttattgata attagctctg ccatctcctt
    30781 gtctttggag gctaaggcca gtaaggcttt tgaagctgtt tcaaggacaa gttgccctag
    30841 tcctaaaaag gattgttcta attcatgcaa ttctaagtca aattgatttc tcataggttt
    30901 ttccttcctt atccgaaccg tcctgaaata tagtcttctg tgcgctgatc ttttggattg
    30961 gtaaacacgt caacggtatc tccaaattcg caaatttctc ctgttaagaa aaaagcagtt
    31021 ttatctgaaa tacgtgaagc ttgttgcatg ttatgggtaa caatgataat cgtataatcc
    31081 ttttttagtt gctgaatgag gtcttcaatt tttaaagtgg agatagggtc taaggctgaa
    31141 gtcggctcat ccattaacag aacatcaggt tctactgcta aagctcgcgc aatgcaaagg
    31201 cgttgctgct gaccgccaga taaggacatg gcactctttt taagatcatc tttgacttct
    31261 tcccaaatgg ctgccccttt taaagatttc tccactaagg catctaattg ttttttgtct
    31321 cgaataccat gtgtccttgg accataagcc acgttatcat agatagacat ggcaaaggga
    31381 ttaggctgtt gaaaaaccat ccctacacgc ttgcgtagct gattaaggtt gaatttgcta
    31441 ctataaatat cttgctcatc taagaggact tggccttcaa tatggcaaga aggaaccaaa
    31501 tcgttcatcc ggttaagggt ttttagaaaa gttgatttgc cacaaccaga tgggcctatc
    31561 aaggcagtaa tctgtctttc tggtaattga atcgaaatat tttttaaggc ttgaaaatcc
    31621 ccgtaaaata agtctaggtg tctgactgaa aatgttccca tactaggaag ctcctttcac
    31681 aagttttcga gataataagc ttgatagagt atttatcatt aaaacagtaa taatcaaaat
    31741 cacaccggta gcataggctt cattgacatg tagcccctca cttgacagca tatacatatg
    31801 cagggctaga gaacgacctg aagacatgag actacttggc gtattggtag aggtacctaa
    31861 tgtatacatg agggcagctg tctcaccaac gatacggcca atagctagta tcactccagc
    31921 taaaatacct ggcatggcaa ctggtagaac aattctaaaa acagtccgta acttacctgc
    31981 cccaagtcca taacttgctt gacgcatgct atcactaaca gataaaaggg cttcttctgt
    32041 tgagcgaata atgactggca acaccatgat gactgaggtt aagattcctg ataacagaga
    32101 gtattgaaaa cctaagaaga ctacaaagaa gagcatgcca aacagaccaa aaacaatgga
    32161 aggaatccca gacaaggtat ctgaggccaa tcgcatgatt ttaacacaaa gggaatcttt
    32221 ttttgtatat tccacaagat aaaaaccagc aaaaatccct atgggcaagg ctaaaagaag
    32281 agcaccaaag accagaataa cggtggaaat aatcgctggc ataagggaaa tgttctcaga
    32341 agtataagtc caagaaaaga gggatagact tagatgaggt aagcctttga tgaggataaa
    32401 accaatgatt aaaaagagag agccaaaggt taaagctgaa aaacaataaa cgagaagttt
    32461 tagcaggtat ttactcataa gatgattttc ctttcaagta ggcaaagtag gcattaatca
    32521 agagaataag gaaaaagaga actgctgagg ttgcaataag ggcttcccta tgctgacctg
    32581 atgcgtaagc catttccaga acaatattgg ttgttaaggt tctggttcct gaaaagagtc
    32641 cacttggaat aatcggctgg ttgcctgcca ccaaaataac tgccatggtt tcacctactg
    32701 cgcgaccgat tcctaaaata actgctgata aaataccaga tctcgcagct ggcaagatga
    32761 cactaaaaat actccgttca tgactagctc ctagagccaa gctaccagaa taatacgttt
    32821 tgggaactgt tcggatagca gattctgaca aactgataat ggttggcaaa atcattattc
    32881 ctaatagtaa cgaagcggtt aggacactca tgccatttcc taaaaaggtt ctaatccaag
    32941 gcaccaataa ttgtaggccg aaaaaaccat aaacaataga tggaatggcc gccatcaagt
    33001 tgatagctga ttttaagaag ccatagacgg gctttggaca ataataaacc ataaacaccg
    33061 atgtcaagat gcctgttggc accccaatca caatcgctcc taaggtaatt aataaggaac
    33121 caacgatcat tggtaaaata ccatagcttg ccggaatgtt cgttggcgac caatcactgc
    33181 ctaataaaaa acgggcaaag ccgtagttag ccatgaaagg taagccatta ctaaaaataa
    33241 agaaacagat tagcaaaata gctacaacag ctactgttgc actcatgaaa aaaattgccc
    33301 taaaaactgc ttctttgaag gcttgttttg tcacatcttg tcctttctag tgaagaaagt
    33361 aagggagata cgacacctcc ctacttgcct tctttatctt attgtacgat gaaacgtctg
    33421 catctcttta gagatttatg gagcaaacat tttatttaat cttgtcccag gtggttaatt
    33481 tgccactaaa aacgtctgca agttcagcca tactgacttg gcttgcctta ttgtcattat
    33541 tgaccacaac agcaataccg tctaaagcaa tagcatcatg ggtgagactc ttaccttctt
    33601 caggagttaa ttccctagaa accataccaa tatcagcggt tttctcctta acagcggtaa
    33661 tacctgctga agacccatta gaggtaatat caatcgtaac ttctggattt tcttttttat
    33721 aagcttctgc taatttttcc attaaagaag atactgaagt ggaacctaca acagacaact
    33781 tgcctgataa gtgttggctt gtatattctg tggtttcggt tttagcttca ataaatttat
    33841 tatctgtgac cacttgttga ccttgtttgg agtggataaa gctgataaaa tcttgaccta
    33901 gcttggaaag attagaagac caaacaatgt tgaagggacg ttgaagaggg tattcaccat
    33961 ctaaaactgt gtctcgacta gccttgacac catcaatctc taaagccttg acagatttcg
    34021 ttaaagatcc caaggagatg tagccgatag cattagcatt cccttgaact gctgagagaa
    34081 caccttctgt actattttga atcacagctg ttttggcagt gttgtcaatt tttttatcac
    34141 cgtctttttt gagaatccct gtgatttctg tgaaggcacc ccgtgttcca gagccatttt
    34201 ctcgtgaaat cacctcaatc gttcctgaag ctgactgttt ggaagcagct gactgattgc
    34261 cacaggcaac aagcccaaat cctgataagc caatggctgc aagagtaagc atttttttga
    34321 atttcataat aatcaccttt atctctatgt atttttcttg tgtaggctta ctacatttat
    34381 agtctaacaa gtctttgtaa aggtttatcc ctgattcatg taaagattgt gtaaagaatc
    34441 aaaaaaagcc acttttgaaa aatggctgct cctaaaaata gctttaaaaa ttattagtcc
    34501 tgtgcgaaag attggttagg aagaaaaatc gtgaagcaac tgcctctgcc aagctgactc
    34561 gtcaccgtga cttggccacc taataattga ctgagttctt tgacaatggc aaggccaaga
    34621 ccagtgccac cagtttgtct gcttcgacct ttattaactc ggtaaaaacg ttcaaaaata
    34681 cgatcctgct ctaattgact aataccaatc cctgtatctg atacagaaat cttaatgcct
    34741 tcgttcacct tttgggtctt gacctcaatt tttccccctt gttcagtgta acggatggca
    34801 ttggataaaa gattgagtaa gatttgggaa agtaattgac tatctgatac gagggtgaca
    34861 tcatctggca cctgcacctt tagctgtaaa tccttcttct tgagctgagg ttgcaagctt
    34921 tgagtcaaat cctgtacaaa ttctgccaaa gaaagggtcg tccattgtat aggcatttgt
    34981 tgagccttag ataaggtaag aagatgctca acaatatgct caagacgcaa gctttctttg
    35041 taaataatgt ctagaaagtc atccttgagc gcttcttctt cagctgacat ccccttaatg
    35101 gtttcagcaa agcccttaat cgaagtaact ggtgtcctca attcatggga ggcatttgag
    35161 acaaaggcta aatttaactt ttcataagtt ctaatcgttg ttaaatcata tagcaagacg
    35221 agcacagctt ccacagattg ggtggggcta aaaacgggaa ctgctgtcac ttctaaaatc
    35281 aagtcaccct catgaaaccc acttacttct tgttttaacc ttgttttttg atcaaaggct
    35341 tggtgaacta aatttcgaat atccatccgt ttgaggtcat caagtgaact tatgtcgccg
    35401 tccacatcgg gaaaataatg aggcagagag cgactggata ataacatctg accttgagcg
    35461 gaaactaaaa acgtccccat ggttaggtgc gacagaagaa cctccattgt ttcggctagg
    35521 tccttgtatt gctgatcctg ttgggagact ttggttttta gggcagacac atactgagcc
    35581 aaagacttta agtcttcttg ccctttttct aaaaagtatt cactactggt caagagaggt
    35641 tggtgcaagg tctcaaaagc aacttcccat ttccaaaggc aaaagagcca gtagccacct
    35701 tgtcccaaag aaagggctag aagaaagaga ccgatgcctt tactgatcca agttaatgcc
    35761 atccctgcaa tcagaatgag gctaacactt agattgacta gccaaaattg aaggtagcgt
    35821 ttcatctata actccttgaa cttataacca taaccccgaa tggttcgaat aaattgaggg
    35881 gctttaggat tgtcttcaat tttttccctc aacttaccaa tatgaacgtc caccaaacgt
    35941 gtttcctgcc caaagtcata cccccagata cgttccaaaa gacgctctct agtcagtgtc
    36001 atgttgggat gtttcataag atagagcaag agttcaaatt cttttggggt caaactcagt
    36061 aacttattcg ccttgtagac ttcatgacgc tcagggtata ctttcaaggt cccaaatagc
    36121 caagaatcgt cagcgatatt atctgaatca tctccttctt gttctccttt agttcgcctg
    36181 aggacagcct tgacacgcgc cagcaattct ctagggctaa aaggcttggt caggtagtca
    36241 tcagccccta attccaaggc caaaacctta tcaaattcat cacttttcgc agaaaccatc
    36301 ataattggag ttttgacgcc tttggctctc agccgcttac aaacttccat gccatctaat
    36361 tgtggtaaca tgatatcaag caagataaaa tcaaagggtt ctgtttctgc caaagctaag
    36421 gccttccgtc catttgttac caattgagta gaaaagcctt ccttacttaa atggtagtca
    36481 agcaatttca gaatgtgttc ttcatcatcc actaataaga cttgttttgt catctattat
    36541 ctcctattgg taacattata acacaattat cagaaatcct aacattgcta aatcagatta
    36601 aatttgctta tcaagactag tatctggtca aacgctcaat catctccttg tgctctggat
    36661 aggtcgccag tagatctacc ctttcaaata attcaaaatc ctcaaattca aaaccaggag
    36721 caacaagaca agaaaccaga gcataatcct tatcaactgt tgatccccaa atagtgccct
    36781 taggaacaca gtagtgaagt tgttgccctt tggatatgtc caggcctaaa gtgactgctt
    36841 cgtagtgacc atctgctgta atcatgtgaa cagtaagtgg ggatcctgca tgaaaatacc
    36901 agatttcatc tgctgtcaat cggtgaaaat gtgaaggatt cgtttcttct aataagaaat
    36961 aaatactggt ataaagcgcc cttcccttac cagcaaggtt tatagtgtct gaagcttttt
    37021 ttgtttgtct aaaatagcca ccttcaacat ggggagctaa ctctagagtt cttatcaagt
    37081 cttctttatc cgtcggagcc aatgggttga agtaactctt gttcaaaggt ggtgttacga
    37141 tttcaagaac tcctctcagc tctgaggaca cgataatgat tgatgcgacg gaagtacaaa
    37201 tcaatcgccc taaaaaaaga attagcgaat tattctggta aaaaaatgcc acgctatg
//
LOCUS       DAVBCV010000014        36490 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBCV010000014 DAVBCV010000000
VERSION     DAVBCV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.014"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            1..732
                     /locus_tag="VQ516_000706"
                     /pseudo
     CDS             1..732
                     /locus_tag="VQ516_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(777..1418)
                     /gene="plsY"
                     /locus_tag="VQ516_000707"
     CDS             complement(777..1418)
                     /gene="plsY"
                     /locus_tag="VQ516_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2511187.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            1554..3497
                     /gene="parE"
                     /locus_tag="VQ516_000708"
     CDS             1554..3497
                     /gene="parE"
                     /locus_tag="VQ516_000708"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2511188.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            3917..6388
                     /gene="parC"
                     /locus_tag="VQ516_000709"
     CDS             3917..6388
                     /gene="parC"
                     /locus_tag="VQ516_000709"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2511189.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            6520..7542
                     /locus_tag="VQ516_000710"
     CDS             6520..7542
                     /locus_tag="VQ516_000710"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2511190.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            7598..8302
                     /locus_tag="VQ516_000711"
     CDS             7598..8302
                     /locus_tag="VQ516_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2511191.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSDM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            8367..9056
                     /locus_tag="VQ516_000712"
     CDS             8367..9056
                     /locus_tag="VQ516_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2511192.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            9053..9976
                     /locus_tag="VQ516_000713"
     CDS             9053..9976
                     /locus_tag="VQ516_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2511193.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9991..10635
                     /gene="pcp"
                     /locus_tag="VQ516_000714"
     CDS             9991..10635
                     /gene="pcp"
                     /locus_tag="VQ516_000714"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2511194.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10803..11033
                     /locus_tag="VQ516_000715"
     CDS             10803..11033
                     /locus_tag="VQ516_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2511195.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            11083..11163
                     /locus_tag="VQ516_000716"
     tRNA            11083..11163
                     /locus_tag="VQ516_000716"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11117..11119,aa:Tyr,seq:gta)
     gene            11173..11244
                     /locus_tag="VQ516_000717"
     tRNA            11173..11244
                     /locus_tag="VQ516_000717"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11205..11207,aa:Gln,seq:ttg)
     gene            11406..12608
                     /gene="rpsA"
                     /locus_tag="VQ516_000718"
     CDS             11406..12608
                     /gene="rpsA"
                     /locus_tag="VQ516_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2511196.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            12694..13191
                     /locus_tag="VQ516_000719"
     CDS             12694..13191
                     /locus_tag="VQ516_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2511197.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            13191..14846
                     /gene="dnaX"
                     /locus_tag="VQ516_000720"
     CDS             13191..14846
                     /gene="dnaX"
                     /locus_tag="VQ516_000720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2511198.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            14874..15068
                     /locus_tag="VQ516_000721"
     CDS             14874..15068
                     /locus_tag="VQ516_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2511199.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            15218..15988
                     /gene="sufC"
                     /locus_tag="VQ516_000722"
     CDS             15218..15988
                     /gene="sufC"
                     /locus_tag="VQ516_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2511200.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITRLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            16016..17278
                     /gene="sufD"
                     /locus_tag="VQ516_000723"
     CDS             16016..17278
                     /gene="sufD"
                     /locus_tag="VQ516_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2511201.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            17289..18515
                     /locus_tag="VQ516_000724"
     CDS             17289..18515
                     /locus_tag="VQ516_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2511202.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            18502..18942
                     /locus_tag="VQ516_000725"
     CDS             18502..18942
                     /locus_tag="VQ516_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2511203.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            18992..20404
                     /gene="sufB"
                     /locus_tag="VQ516_000726"
     CDS             18992..20404
                     /gene="sufB"
                     /locus_tag="VQ516_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2511204.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(20635..21876)
                     /locus_tag="VQ516_000727"
     CDS             complement(20635..21876)
                     /locus_tag="VQ516_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2511205.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            21979..23088
                     /locus_tag="VQ516_000728"
     CDS             21979..23088
                     /locus_tag="VQ516_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2511206.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            23622..23969
                     /gene="ssrA"
                     /locus_tag="VQ516_000729"
     tmRNA           23622..23969
                     /gene="ssrA"
                     /locus_tag="VQ516_000729"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24081..24168)
                     /locus_tag="VQ516_000730"
     tRNA            complement(24081..24168)
                     /locus_tag="VQ516_000730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24132..24134),aa:Ser,seq:gga)
     gene            24330..25070
                     /locus_tag="VQ516_000731"
     CDS             24330..25070
                     /locus_tag="VQ516_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2511207.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            25067..25978
                     /gene="pfkB"
                     /locus_tag="VQ516_000732"
     CDS             25067..25978
                     /gene="pfkB"
                     /locus_tag="VQ516_000732"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2511208.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            25975..27927
                     /locus_tag="VQ516_000733"
     CDS             25975..27927
                     /locus_tag="VQ516_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2511209.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            28091..30316
                     /locus_tag="VQ516_000734"
     CDS             28091..30316
                     /locus_tag="VQ516_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2511210.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(30361..30789)
                     /locus_tag="VQ516_000735"
     CDS             complement(30361..30789)
                     /locus_tag="VQ516_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511211.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            30927..32069
                     /locus_tag="VQ516_000736"
     CDS             30927..32069
                     /locus_tag="VQ516_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2511212.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            32078..33292
                     /gene="thiI"
                     /locus_tag="VQ516_000737"
     CDS             32078..33292
                     /gene="thiI"
                     /locus_tag="VQ516_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2511213.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            33427..34251
                     /locus_tag="VQ516_000738"
     CDS             33427..34251
                     /locus_tag="VQ516_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2511214.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            34273..35012
                     /locus_tag="VQ516_000739"
                     /pseudo
     CDS             34273..35012
                     /locus_tag="VQ516_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            35025..36191
                     /locus_tag="VQ516_000740"
     CDS             35025..36191
                     /locus_tag="VQ516_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2511215.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
BASE COUNT        10946 a         6700 c         8150 g        10694 t
ORIGIN      
        1 gagcttaaag ctgaaattca atccattttt atcgaacaca agggaaatta tggttatcgt
       61 cgaatttatt tagaactaag aaatcgtgct tatctggtaa atcataaaag agttcaaggc
      121 ttgatgaaag tactcaattt acaagctaaa atgcgacaga aacgaaaata ttcttctcat
      181 aaaggagacg ttggcaagaa ggcagagaat ctcattcaag gccaatttga aggctctaaa
      241 acaatggaaa agtgctacac agatgtgaca gaatttgcca ttccagcaag tactcaaaag
      301 ctttacttat caccagtttt agatggcttt aacagcgaaa ttatcgccta taatctttca
      361 acttcgccca acttagaaca agtataaaca atgttggaac aggcattcaa agagaagcac
      421 tacgagaata cgattctcca tagtgaccaa ggctggcaat atcaacacga ttcttatcat
      481 cagttcctag agagtaaggg aattcaagca tccatgtcac gtaagggaaa cagcccagac
      541 aatggtatga tggagtcctt ctttgggatt ctgaaatcgg aaatgtttta cggttatgag
      601 aagtcgtttc agtcgcttaa gcaattggaa caagccatta tagactatat tgattactac
      661 aacaataaac gaattaagat aaaactaaaa ggacttagcc ctgtgcaata cagaactaaa
      721 tccttcggat aaattaattg tctaactttt ggggtgcagt tcacagaact ggcattttat
      781 tttttaggat cttgatgggt taggttcaat ccccaaggga ccaaattttc agttttattt
      841 ttgatacgag ctatattgtc cttatgacga atgataatca aactagcaag tgctaagata
      901 atagcgatga agagagagtc atagttactc aggataaaac caaaaagtgg aaagagcaga
      961 accccgataa cagccgcgat cgatgctgtg acactagaca gtgaaatcat actgccaaga
     1021 tagagagctc caaagaagat aatcgcaagg tagagacaga agataggcgc aaatccgaaa
     1081 attactccag cactggttgc gacagcctta ccacctttaa atcctgcaaa gatagggaag
     1141 gtatggccga taacagccaa aagtccaaag atgagaggag aaacgccttg tagatgaaaa
     1201 ataatcggaa gcagcgttgc tagggttcct ttgaaaaagt caatcacaaa ggttgccata
     1261 ccagctttct tacctaaaat gcggaaggta ttggtcgttc cagtgttacc agaaccatgc
     1321 tcgcgtagat tgatttgaaa gaatacttgt ccaatccaga gaccagatgg aatcgaaccc
     1381 agcagatagg ctaggattaa taaaactatt gtaatcatac tcctattata tcatgaattg
     1441 ggaaagaaaa gcagagaatc tctgctaaaa ttgtcacatc ggagaaagaa aaattttgca
     1501 aaatccttgg aaaacctgta gaatagtaaa gatgaacgaa taggaggttc cttgtgtcaa
     1561 aaaaggaaat caatattaac aattataatg atgatgctat tcaggtgcta gaagggttgg
     1621 atgcggtccg aaaacgtcca gggatgtata ttggatcgac cgatggcgct ggtcttcatc
     1681 acctagtttg ggaaatcgtt gataatgcag tcgatgaagc cttgtctggg tttggtgatc
     1741 gtatcgatgt aaccatcaat aaagacggta gtctaacggt tcaagaccat ggacgtggga
     1801 tgccgacagg tatgcacgct atgggaattc caactgttga ggttatcttt accattcttc
     1861 atgccggagg gaaattcggt caaggtggct ataagacatc aggtggactt cacggagtgg
     1921 gttcttccgt tgttaacgcc ctttctagct ggttagaagt tgaaattacc cgtgatggcg
     1981 cagtttacaa gcaacgtttc gaaaatggtg gaaaacctgt cacgactttg aagaaaatcg
     2041 gtacagcacc caagtctaaa acaggcacca aagttacttt tatgcctgac gcgactatat
     2101 tttctacgac agatttcaag tacaatacca tttcagagcg ccttaatgaa tcagcctttc
     2161 tcttgaaaaa tgtgaccttg tctttaacgg acaagcgaac agatgaagcg attgagttcc
     2221 actatgagaa tggagtacaa gattttgttt cttatctcaa cgaagataag gaaatcttga
     2281 cgccagttct ttactttgaa ggggaagaca atggttttca agtggaagta gccctccagt
     2341 acaatgacgg attctcagat aacattctat cctttgtcaa taacgttcgc accaaggacg
     2401 gtggaacgca cgagacagga ctcaagtctg ccattaccaa ggtcatgaat gactatgcac
     2461 gtaaaacagg tcttctcaag gaaaaagata aaaaccttga aggttcagac tatcgtgagg
     2521 gactagcggc cgttctttct atcttagttc ctgaagaaca cttgcagttt gaaggacaga
     2581 ccaaggataa actaggaagc cccctagctc gcccagttgt ggatggaata gtggctgata
     2641 agttgacctt tttccttatg gaaaatgggg aattagcttc taacctcatc cgcaaggcta
     2701 tcaaggcccg tgatgctcgt gaagcagcac gtaaggcgcg tgatgagagc cgaaatggga
     2761 agaaaaacaa gaaagataag ggcttgttgt ctgggaaatt gaccccagcc caatctaaga
     2821 atcctgctaa gaatgaactc tatctagttg agggggactc tgccggtggt tctgccaaac
     2881 aaggtcgtga ccgcaagttc caggctattc tacctcttcg tggtaaggtt atcaatacag
     2941 ccaaggccaa gatggcggat atcctcaaaa atgaagagat caataccatg atttatacca
     3001 ttggtgcggg tgttggagca gacttctcta ttgaagatgc caactatgat aagatcatta
     3061 tcatgaccga tgcggatacc gacggtgccc atatccagac cttgctcttg acatttttct
     3121 accgttacat gcgtccgcta gtcgaggcag gtcatgtcta tattgccctc ccacctcttt
     3181 acaagatgtc caaaggtaaa ggaaagaaag aagaagtggc ctacgcttgg acggacggag
     3241 aactagaaga actccgtaaa cagttcggta aaggcgctac cctccaacga tacaaaggac
     3301 ttggtgagat gaatgcggac cagctctggg aaacaaccat gaacccagaa acacgtaccc
     3361 tcatccgtgt cacaattgaa gatttagcgc gcgccgaacg ccgcgtcaat gttctcatgg
     3421 gagataaggt agaaccacgc cgtaaatgga ttgaagataa tgtcaagttt acgctagaag
     3481 aagcgacagt gttttaatga aagaaaataa tatgttagta aactggaaat tagagtcgga
     3541 tgtgaatgac tatgtgaaaa agcagtttga aaatcttggt ttgaaaaaat tgcaggatta
     3601 ttaatgaaga atctgctatg agtgcttatc tcaaagaggc gcttaaggga gcggctaaga
     3661 cacaaactaa gactaatttt ggaaaaccag attttcatat tgaaaaatac aaacaggata
     3721 gtttggttac ttatcgtaac tgaacacggg aggaattccc gtaaaatatc ttttactgca
     3781 taaaaaccta ctctacattc tttgaaagga gttgaacacg ccctagatac tgtgtgaaaa
     3841 agataaattc tcttgtgagt ttgcttactt caagaatttt ctattttcac ttggtatttt
     3901 atgggctttg tatcttatgt ctaacattca aaacatgtcc ctggaggaca tcatgggaga
     3961 gcgctttggt cgctactcca agtacattat tcaagaccgg gctttgccag atattcgtga
     4021 tgggttgaag ccggttcagc gccgtattct ttattctatg aataaggata gcaatacttt
     4081 tgacaagagc taccgtaagt cggccaagtc agtcgggaac atcatgggga atttccaccc
     4141 acacggggat tcttctatct atgatgccat ggttcgtatg tcacagaact ggaaaaatcg
     4201 tgagattcta gttgaaatgc acggtaataa cggttctatg gacggagatc ctcctgcggc
     4261 tatgcgttat actgaggcac gtttgtctga aattgcaggt taccttcttc aggatatcga
     4321 gaaaaataca gttccttttg catggaactt tgacgatacg gagaaagaac caacggtctt
     4381 gccagcagcc tttccaaacc tcttggtcaa tggttcgact gggatttcgg ctggttatgc
     4441 cacagacatt cctccccata atttagctga ggtcatagat gctgcagttt acatgattga
     4501 ccacccaact gcaaagattg ataaactcat ggaattcttg cctggaccag acttccctac
     4561 aggggctatt attcagggtc gtgatgaaat caagaaagct tatgagactg ggaaagggcg
     4621 cgtggttgtt cgttccaaga ctgaaattga aaagctaaaa ggtggtaagg aacaaatcgt
     4681 tattactgag attccttatg aaatcaataa ggccaatcta gtcaagaaaa tcgatgatgt
     4741 tcgtgttaat aacaaggtag ctgggattgc tgaggttcgt gatgagtctg accgtgatgg
     4801 tcttcgtatc gctatcgaac ttaagaaaga cgctaatact gagcttgttc tcaactactt
     4861 atttaagtac accgacctac aaatcaacta caactttaat atggtggcga ttgacaattt
     4921 cacacctcgt caggttggga ttgttccaat cctgtctagc tacatcgctc accgtcgaga
     4981 agtgattttg gcgcgttcac gctttgacaa agaaaaggct gagaaacgtc tccatatcgt
     5041 cgaaggtttg attcgtgtga tttcgatttt ggatgaagtc attgctctta tccgtgcttc
     5101 tgagaataag gcggacgcca aggaaaacct caaagttagc tatgatttta cggaagaaca
     5161 ggctgaggct atcgtaactt tgcaactgta ccgtttgacc aataccgatg tggttgtctt
     5221 gcaggaagaa gaagcagagc ttcgtgagaa gattgctatg ctggcggcta ttatcggtga
     5281 tgaaaggact atgtacaatc tcatgaagaa agaacttcgt gaggtcaaga agaaatttgc
     5341 aactcctcgt ttgagttctt tagaagacac tgcgaaagca attgagattg atacagctag
     5401 tcttatcgct gaggaagata cctacgtcag cgtgaccaag gcaggttaca tcaagcgtac
     5461 cagtccacgt tcctttgcgg cttccacctt ggaagaaatt ggcaagcgtg atgatgaccg
     5521 tttgattttt gttcaatctg ccaagacaac ccagcacctc ttgatgttca caagtcttgg
     5581 aaatgtcatc tacagaccaa tccatgagtt ggcagatatt cgttggaagg acatcggaga
     5641 gcatctgagc caaaccatca caaactttga aacgaatgaa gaaatccttt atgtggaagt
     5701 actggatcag tttgacgatg cgacaaccta ctttgcagtg actcgccttg gtcaaatcaa
     5761 acgggtagag cgaaaagaat tcactccatg gcggacctat agatctaagt ctgtcaagta
     5821 tgctaagctc aaagacgata cagatcagat tgtagcagtg gctccgatta aactagatga
     5881 tgttgtcttg gttagtcaaa atggttatgc cctgcgtttc aatatcgaag aggttccggt
     5941 tgtcggtgct aaggcagcag gtgtcaaggc tatgaatttg aaagaagatg atgtcctcca
     6001 atctggcttt atctgtaata cttcgtcctt ctacctcttg acccagcgtg gaagcttgaa
     6061 acgtgtttct attgaggaaa ttctagcaac cagccgtgcc aaacgaggat tacaagtctt
     6121 gcgtgagttg aaaaacaaac cgcatcgtgt cttcttggca ggagcagttg cagagcaagg
     6181 atttgttggc gatttcttca gtacggaagt ggatgtgaac gaccaaactc tgcttgtcca
     6241 atccaataaa ggaacaatct atgaaagccg attgcaagac ttgaacttgt cagaacgcac
     6301 tagcaatgga agcttcattt ctgacacgat ttcagatgaa gaagtttttg acgcttatct
     6361 tcaggaagta gttactgaag ataaataagt gaaatgaaga atcagttcta agagctggtt
     6421 tttattggag aaattttctg aaaattacaa aatatacttg ctattttaga aaaatagtgt
     6481 agaatataag aaataggttt ttagaataag gagtggaata tgacagtaac gattgattgg
     6541 gaaaacctcg gtttttccta tatgaaatta ccttatcgct atcttgctca tttcaaaaat
     6601 ggacaatggg atcaaggaga gcttacagag gatgcaactt tgcatatttc agagtcttct
     6661 ccaagtcttc actatggaca acaagcattt gaaggtttga aagcttatcg tactaaggat
     6721 ggcagtgttc aactgttccg tcctgatgaa aatgctaaac gtctgcaacg tacatgtgac
     6781 cgtctcttga tgccacaagt tccgacagac atgtttgtag aagcttgtaa agcagttgtc
     6841 cgtgcgaatg aagaatacgt accaccatac ggaacaggtg gaaccttata tcttcgccct
     6901 cttttgattg gtgtcggaga tattatcggg gtaaaaccgg cagaagagta cattttcacc
     6961 atctttgcta tgccagttgg aaattacttt aaaggtggtt tggtcccaac caacttcttg
     7021 attcaggatg agtacgaccg tgcagcacca aatggtacag gtgcggctaa ggttggtgga
     7081 aactatgctg caagtctctt accaggaaaa atggccaagt cacgtcattt ctcagatgtt
     7141 atctatctgg acccatcaac tcatacaaag attgaagaag tcggatcagc taacttcttt
     7201 ggaattacag ctgataatga atttgtaaca ccattgagtc catctatctt gccatctatt
     7261 accaagtatt ccttgcttta tttggcagaa catcgcttgg gattaactcc tattgagggt
     7321 gatgttccaa ttgataatct tgaccgtttt gtagaggcag gtgcctgtgg tacagcagcg
     7381 gttatttctc caattggagg tattcaacat ggtgatgatt tccatgtatt ctatagtgaa
     7441 acagaagtag gtcctgtgac gcgtaaatta tataatgaat tgacgggtat tcagtttggc
     7501 gatattgaag cgccagaagg ttggattgta aaagtagatt aaaataaacc aaaggagatt
     7561 ttttatgaaa tagaaaaagt ggctcttaac agcaggagtg gtcctgagca cgtcagctat
     7621 tttagtggct tgtggaaaaa ctgataaaga agcagatgca ccgacaacat tttcttatgt
     7681 ctatgcagta gatccagcat cattgggcta cagtatagcg actcgaacat cgaggacaga
     7741 cgttattgga aatgttattg atggtttgat ggaaaatgat aaatacggca atgttgctcc
     7801 ttctcaaaaa gactatgatt tgaacagtac aggatgggct ccaagctatc aagatccagc
     7861 gtcttacttg aatattatgg atccaaaatc tggttctgac atgaaacacc ttggcattac
     7921 gaaaggaaaa gataaggatg ttgtagctaa acctggtttg gataaatata agaaattgtt
     7981 agaagatgct gtttctgaga ccactgacct agagaagaga tatgaaaaat atgccaaagc
     8041 tcaagcttgg tcgacagata gttcattatt gatgccaaca gcttcatctg gtggttttcc
     8101 agttgtaagt aacgtagtac cattctcaaa accatactca caagttggta ttaaggggga
     8161 accatatatc tttaaaggaa tgaaattgca aaaagatatt gttacaacaa aagaatataa
     8221 cgaggttttt aaaaaatggc aaaaagaaaa attggaatcc aatagcaaat accaaaaaga
     8281 actagaaaaa tacattaaat aaggaatggt attgatcttg ataaaatttt taaaatactg
     8341 tcattttgaa tataaaggag tttgatatgg agtggattag attaatagga atagcaatca
     8401 ttgttgtggg ttttatttta aaatttgata caattgcaac agtagtctta gctggtttgg
     8461 ttacagcttt agtttcaggt gtttctctcg ttgaattttt ggagattttg ggaaaagaat
     8521 ttagcaatca gcgagtgctc acgattttta tggttacctt gcctcttgtg gggctgtcag
     8581 aaacctttgg actcaagcaa cgatcaatcg atttgattcg aaagattaaa ggtctgacag
     8641 ttggaaactt ctatacagtt tatttcttta ttcgagagtt agctggtttc ttttcaattc
     8701 gtctaggagg acaccctcag tttgtcagac ctttggttca acctatggga gaagcagctg
     8761 cagagtctca attaggtaga aagttaacag aggttgaaga tgagacaata aaagcgcgtg
     8821 cggctgcgaa tgaaaatttt ggaaatttct ttgctcaaaa tacgtttgta ggtgctgggg
     8881 gagtcctctt gataggggga acattagatc agttaggcta tgaaagcaat tatgcaggga
     8941 ttgcttctac atctattatt gttgctgtta taacacttat tgtagtgggg atttacaatt
     9001 atttatttga taaaaaattg atatcaaaaa agactagggg aggagaacaa aaatgacaga
     9061 gttggcaaag caactattag agttgaccta tattgtgatt ggttgtcaat ttctccatac
     9121 agcctattgt agttataaag ataaaacaaa cccagttcga cttgggacat ctgcattttg
     9181 gactctattg tctattacgt ttataggtgg ttcctatatg ccaaatatga gtattggtat
     9241 tattgtaatc ctattatcgc tgttaacatt gtttaagcaa gtccgtatcg gaaccttgcc
     9301 atccttagat gaaatgaaag ccaatattga atctaacagg ttgaaaaata aaatttttat
     9361 tccagttatg ctgatggcaa tacttgcgtt ggtcttagcg caaatgattc cagaatttag
     9421 caagatttcg attagccttg ccgccttgtt tgctacaatt tctgttcttg tgattaccaa
     9481 tagtcaccct aagagtctgt tatcagaaaa taatcgaatg actcagcaag tttcaacaag
     9541 tgggattgtt cctcaattat taggggcttt gggggctatt tttactgtag caggtgttgg
     9601 tgatgttatc tctcatctga ttagcggtat tgttccttca gatagtcgct ttataggagt
     9661 tttggcttat gttcttggaa tggttctatt cacaatgatt atgggaaatg cttttgcagc
     9721 attcaccgtt attacagcag gtgttggagt tccctttgta tttgctctgg gagctaatcc
     9781 aattgtggct ggtgctcttg ccatgacagc aggttattgt gggaccttat tgaccccaat
     9841 ggctgctaat tttaacgctc taccagcagc attgatggat atgaaagatc agaatggcgt
     9901 tataaaggct caagcaggtg ttgctctagt aatgattgtt attcacatat tcttaatgta
     9961 ctttctcgca ttttagtaaa ggaaaataaa atgaaaatat tggttacagg ttttaatcct
    10021 tttggaggtg aaaagattaa tccagctttg gaggctgtaa aattattacc atctgagatt
    10081 aatggggctg aagttcgctg ggtagaaatt ccaacggttt tttataagtc gtcagaagtt
    10141 ttagaggcag aaatattacg atatcaacca gatgctgtac tttgtattgg acaagcaggc
    10201 ggcaggaccg gcttaacacc tgaacgagtg gctattaatc aagatgatgc tcgcatacct
    10261 gataacgaag gcaatcaacc aattgataca ccgattcgta ttgatggagc atcggcctat
    10321 tttagtagtt tacctatcaa agcgatggta caagctatca aaaaacaggg acttccggca
    10381 gttgtatcca atagtgcagg aacctttgtt tgcaatcatt tgatgtacca agctctttat
    10441 ttagtggata aaaaattccc taatatgaga gcaggtttca tgcatattcc atatatgatg
    10501 gaacaagtag taaataagcc gaatactgca ggaatgagtc tatgtgatat tgtaagaggc
    10561 atagaggtag ctattgaagc tattgtagac tacaaagata aggatttgca gttagtaggc
    10621 ggtgagactc actgataacc taaaaaggat agtcaattat gcttgtttac taactattaa
    10681 ctatgctaaa tcaattgagg ttgtttacat aaaactctat atcagagaag cctgatatag
    10741 agttttttct tgctagtttt aggattttct tgtaaaatag aaaaagtgaa gagaggtatg
    10801 aaatgagcaa gaaagataaa aaaatcgaaa ttcaagtagc ggatgccaaa gttaatgttg
    10861 gtaaagacag ttttgaaggt tatacattga ctatcggtaa aaaagttatc ggagaaattg
    10921 ccgaattaga cggacaattt gccattataa agaatgggaa tgtcgatagt ttttataaaa
    10981 aattggaaaa agctgtggaa attttgattg aaaattataa tttagcaaaa taagtcttgt
    11041 ttttttgaaa ttttcatgat ataatagtcc atgttgattg taggagagat agcgaagagg
    11101 ctaaacgcgg cggactgtaa atccgctcct tcgggttcgg gggttcgaat ccctctctct
    11161 ccatttcatt aatggggtat agccaagcgg taaggcaagg gactttgact ccctcatgcg
    11221 ttggttcgaa tccagctacc ccagttctta ggtaataatc aagataaaaa gcaaaatatc
    11281 ttagggtatt ttatttttat aattgaaaga cgtgaatgat atgaacatgt ccttgcgggt
    11341 gcttaggaaa aaaattataa gtatgtcaag tttaagaaaa acttgattgt tggaggattt
    11401 tttagatgaa cgaatttgaa gatttgctaa atagcgttag tcaagttgag actggtgatg
    11461 ttgttagtgc tgaagtattg acagttgatg cgactcaagc taacgttgca atctctggaa
    11521 ctggtgttga aggtgtcttg actcttcgcg aattgacaaa cgatcgtgat gcagatatca
    11581 atgactttgt taaagtagga gaagtattgg atgttcttgt acttcgtcaa gtagttggta
    11641 aagatactga tacagttaca taccttgtat ctaaaaaacg ccttgaagct cgcaaagcat
    11701 gggacaaact tgttggtcgc gaagaagaag ttgttactgt taaaggaacg cgtgccgtta
    11761 aaggtggact ttcagtagaa tttgaaggtg ttcgtggatt tatcccagct tcaatgttgg
    11821 atactcgttt cgtacgtaac gctgagcgtt ttgtaggtca agaatttgat actaaaatca
    11881 aagaagttaa cgctaaagaa aaccgcttca tcctttcacg tcgtgaagtt gttgaagcag
    11941 ctactgcagc agctcgcgct gaagtattcg gtaaattggc tgttggtgat gttgtaactg
    12001 gtaaagttgc tcgtatcaca agcttcggcg ctttcatcga ccttggtggt gttgacggat
    12061 tggttcactt gactgaattg tcacatgaac gtaacgtatc accaaaatca gttgtaactg
    12121 ttggtgaaga aattgaagtg aaaatccttg atcttaacga agaagaagga cgtgtatcac
    12181 tttcacttaa agcaacagta ccaggaccat gggatggcgt tgagcaaaaa ttggctaaag
    12241 gtgatgtagt agaaggaaca gttaaacgtt tgactgactt cggtgcattt gttgaagtat
    12301 tgccaggtat cgatggactt gttcacgtat cacaaatttc acacaaacgg attgaaaatc
    12361 caaaagaagc tcttaaagtt ggtcaagaag ttcaagttaa agttcttgaa gttaacgcag
    12421 atgcagaacg cgtgtcactt tctattaaag ctcttgaaga acgtccagcc caagaagaag
    12481 gacaaaaaga agaaaaacgt gctgctcgtc cacgtcgtcc aagacgtcaa gaaaagcgtg
    12541 atttcgaact tccagaaaca caaacaggat tttcaatggc tgatttgttt ggtgatatcg
    12601 aactttaatc aaattgaaaa ttcacaaaat cctttgttta ctaaacaagg gatttttctt
    12661 ttgtctctgc tccttttttg atataatagt tctatgttaa aatcagaaaa acaatcacgt
    12721 tatcaaatgt taaatgaaga attgtccttc ctattggaag gcgaaaccaa tgttttggct
    12781 aatctttcca acgccagtgc tcttataaaa tcacgttttc ctaataccgt atttgcaggc
    12841 ttttatttgt tcgatggaaa ggaattggtt ttaggtccct tccaaggagg tgtttcctgc
    12901 atccgtattg cactaggcaa gggtgtttgt ggtgaggcag ctcactttca ggaaactgtt
    12961 attgttggag atgtgacgac ctatctcaac tatatttctt gtgatagtct agctaaaagt
    13021 gaaattgtgg tgccgatgat gaagaatggt cagttacttg gagttctgga tctggattct
    13081 tcagagattg aggattacga tgctatggat cgagattatt tggaacaatt tgtcgctatt
    13141 ttgcttgaaa agacaacatg ggactttacg atgtttgagg aaaaatctta atgtatcaag
    13201 cactttatcg aaaatataga agtcaaaact tctcccagtt agttggtcaa gaagttgtgg
    13261 ctaagactct taaacaagcg gtggagcaag agaaaataag tcacgcttat cttttttctg
    13321 gtcctcgtgg aacgggaaaa accagtgttg ctaaaatctt tgccaaggct atgaactgtc
    13381 ccaatcaagt gggtggcgaa ccttgcaata actgctatat ttgtcaagca gtgacggacg
    13441 gtagtttaga agatgtcatt gaaatggatg cagcttctaa taatggggta gatgaaattc
    13501 gcgaaattcg tgataaatct acctatgcgc ctagccttgc tcgttataag gtttatatca
    13561 tagatgaggt tcacatgctg tctacagggg cttttaatgc cctcctaaag acgctggaag
    13621 aaccaacaca gaatgtagtc tttattttgg ccactactga attacacaag attcctgcta
    13681 ctattctatc ccgtgtgcaa cgttttgagt ttaaatcaat taagacacag gatattaagg
    13741 aacatattca ctatatctta gaaaaagaaa atatcagttc tgaaccagag gctgtggaaa
    13801 tcattgccag acgggcggaa ggtggaatgc gggacgcctt gtctattttg gatcaagccc
    13861 tgagtttgac acagggaaat gagctgacga ctgctatctc tgaagaaatt actggcacta
    13921 ttagcctatc agccttggat gattatgtgg cggccttgtc tcaacaggat gttcccaaag
    13981 ctttgtcttg cttgaatctt ctttttgaca atggtaagag catgactcgt tttgtgaccg
    14041 atcttttgca ctatttaaga gacttgttaa ttgttcaaac agggggagaa aatactcatc
    14101 atagttcagt ctttgtagaa aatttggcac ttcctcaaaa aaatctgttt gaaatgattc
    14161 gcttagcaac agtgagttta gcagatatta agtctagttt gcagcccaag atttatgctg
    14221 aaatgatgac cgtccgtttg gcggaaatca agcccgaacc agctctatca ggagcggttg
    14281 aaaatgaaat tgctacgctg agacaggaag ttgcccgtct caaacaagag ctttctaatg
    14341 taggtgcggt tcctaaacaa gttgcaccag ctcctagtcg accagctacg ggcaaaacag
    14401 tctatcgtgt cgatcgcaat aaagtgcaat ctatcttaca agaggccgtc gaaaatcctg
    14461 atttagcacg tcaaaatcta attcgtttgc agaatgcctg gggagaggta attgaaagtc
    14521 taggtgggcc ggacaaggct ctgctagttg gttctcaacc ggttgctgcc aatgaacacc
    14581 atgctattct tgcttttgag tctaacttca atgctggtca aactatgaaa cgagacaatc
    14641 tcaataccat gtttggcaat atcctcagtc aggcggcagg tttttcacct gagattttag
    14701 ctatttccat ggaggaatgg aaagaagttc gcgcagcctt ttcagccaaa gccaaatctt
    14761 ctcaaactga aaaagaagta gaagaaagcc tgattccaga aggatttgaa tttttggctg
    14821 ataaagtgaa ggtagaggaa gactaaagaa agatttcgtg atacaataag tttatgaata
    14881 aacaacaatt tattattatg gcgctgttta cagctgctga gacctatttt ttcaatgaag
    14941 cctggatgac tggccgctat attatggcag ccttttgggc aattttactc tttagaaatt
    15001 tccgagtcag ttatgtgatg ggcaaaatcg ttgatgtcat cgatcagcat tttaatagga
    15061 aagactagcc ctcagcttcc agacaaaatc aaagcctttt aggctttttt ttgttatact
    15121 agaaaagtat atttatagaa tttttgctct atttctgggg aaatcagacg tttttctagt
    15181 aagtactgta aaagttttga aaaagaaagg aactatcatg tcagtattag agatcaaaga
    15241 tcttcacgtt gagattgaag gaaaagaaat tttaaaaggg gttaacctga ccctgaaaac
    15301 aggagaaatt gccgctatca tgggaccaaa tggtacaggt aaatcgactc tttctgccgc
    15361 tatcatggga aatccaaact atgaagtaac taaaggtgaa gttttgtttg atggcgtaaa
    15421 catccttgag ttggaagtgg atgagcgtgc gcgtatggga cttttccttg ctatgcaata
    15481 cccatcagaa atccctggaa ttaccaatgc tgagtttctt cgtgccgcta tgaatgcggg
    15541 taaagaagat gatgagaaga tttcagttcg tgagtttatt actaggctag atgaaaaaat
    15601 ggaattgctc aacatgaaag aagaaatggc agagcgttac ctcaacgaag gcttctctgg
    15661 tggtgagaaa aaacgcaatg aaattcttca acttttgatg ttggagccaa catttgctct
    15721 tttggacgag attgactcag gtcttgatat tgacgctctt aaagttgtgt ctaaaggtgt
    15781 caatgccatg cgtggtgaag gttttggtgc tatgatcatc actcactacc aacgtctttt
    15841 gaactatatc acacctgatg tggtacacgt gatgatggaa ggtcgtgttg tcctttctgg
    15901 tggtccagaa ttggctgcgc gtttggaacg tgaaggatac gcaaaattag ctgaagaact
    15961 tggctacgac tacaaggaag aattgtaatt tcctcgtatc ttttaggaga agtaaatgac
    16021 tagagaaaat attaaacttt tttcagaaat gcacgctgaa ccaagctggt tggctgatct
    16081 ccgtcaaaaa gcttttgaca agattgagac tttggaatta ccagttattg agcgtgtcaa
    16141 attccaccgt tggaatctgg gtgatggaac gattacagaa aatgagccat cagcaaatgt
    16201 tccagatttc acagctttag atcatcactt gaagttggtg caagtaggaa ctcaaactgt
    16261 tttcgaacaa actccagttg agttagctga acagggtgtt gtcttcacag actttcactc
    16321 agctttagaa gaaattccag agctgatcga agaattcttc atgtcatctg ttaagtatga
    16381 tgatgacaag ttggcggctt accacacagc ttactttaac agtggtgctg tactctatat
    16441 tccagataac gtagaaatca cagagccaat tgaaggaatt ttctaccaag atagcgatag
    16501 caatgtgccg tttaacaagc atattatgat tatcgctggt aaaaattcta agattaatta
    16561 tctggagcgt ttagagtcac gcggtgaagg aagtgccaaa gcaactgcta atatcacagt
    16621 agaagtgatt gcacgttcag gtgcgcaagt caagtttgct gctatcgacc gtctaggtga
    16681 aaacgtcact gcctacatta gccgtcgtgg taaattaggc aacgatgcaa gtattgactg
    16741 ggctatcggt gtcatgaacg aaggaaatgt cgttgctgat tttgatagtg acttgattgg
    16801 taatggtagc tatgctgacc tcaaggttgt agctctttca agtggtcgtc aggtacaagg
    16861 gattgatact cgtgtaacta actatggctg caactctatc ggaaatatcc tacaacatgg
    16921 ggttatcctt gaaaaagcaa ctttgacttt caatggtatc ggccacatca tcaagggtgc
    16981 taagggagca gatgcccaac aagagagccg tgttctcatg ctttcagacc aagcgcgttc
    17041 agatgctaac ccaattcttt tgattgatga aaatgatgtc actgcaggac acgcagcgtc
    17101 tatcggacaa gttgatccag aagacatgta ttacctcatg agtcgtggat tggataaggc
    17161 aactgcagag cgtttggttg ttcgtggttt ccttggatct gttatcgtgg agattccagt
    17221 caaggaagtt cgtgatgaaa tgattgcaac tatcgaagag aaattgtcaa aacgctaagg
    17281 ggaagcctat gttagatgta gaagcgattc gcaaggattt tccaatttta gatcagattg
    17341 tcaatgatga acctctggtc tatctggaca atgctgcgac gacacaaaaa ccactagtag
    17401 ttctgaaagc tattaacagt tactatgagc aggacaatgc caatgttcac cgtggtgtcc
    17461 ataccttagc ggaacgagcg acagcttctt atgaagctgc tcgtgaaacc attcgtaagt
    17521 ttattaatgc aggctctaca aaggaagttc tctttaccag aggaacgaca accagcctta
    17581 actgggtggc acgctttgct gaggaaattc tcactgaggg agaccaggtc ttgatttcag
    17641 taatggaaca ccattctaat atcattccat ggcaggaagc ttgtcgaaag actggagcag
    17701 agcttgtcta tgtctatctt aaagacggtg ccttggatat ggaggatttg cgagctaaat
    17761 tgactgataa ggttaaattt gtttccctag ctcatgcctc caatgttctt ggtgtggtca
    17821 atccgatcaa ggaaatcact caattagccc accaagttgg ggcaatcatg gtagtggatg
    17881 gtgctcaatc tacacctcat atgaagattg atgtccagga cttggatctg gactttttcg
    17941 ccttttcggg tcacaagatg gctggtccga ctggtatcgg tgtcctttac ggcaaagaaa
    18001 agtatcttga gcaaatgtct ccagtagaat ttggcggcga gatgattgat tttgtctacg
    18061 agcaatccgc tagttggaag gaattgcctt ggaaatttga ggctggaacg ccaaatatgg
    18121 caggagctat tggacttgcg actgcagttg attatctgga aaagattggt atggatgccg
    18181 ttgaagctca tgaacaggaa ttgattgcgt acgtctatcc aaaactgcag gcaattgagg
    18241 gattgaccat ttacggttct caggatttgg ctcaacgttc gggtgttatt gcctttaacc
    18301 taggtgatct ccatcctcac gatcttgcga cggctctgga ttatgaagga gtggctgttc
    18361 gtgctggtca ccattgtgcg caacccttgc ttcagtattt ggaagtccca gcatcagctc
    18421 gtgcaagttt ttatatctat aataccaagg cagattgcga caaactagtc gatgccctac
    18481 aaaagacaaa ggagtttttc aatggcactt tctaaactag atagccttta tatggcagtg
    18541 gtagcagacc attcgaaaaa tccacatcac caagggaagt tagaagacgc tgagcaaatc
    18601 agtctcaaca atccgacttg tggggatgtc atcaacctct ctgtcaagtt tgatgcagag
    18661 gaccgtttgg aagatattgc ttttctaaat tcaggatgca cgatttcaac tgcttctgct
    18721 agtatgatga cagatgccgt tttaggaaaa accaaacaag aaattttaga actggcgact
    18781 attttttctg aaatggttca agggcaaaaa gatgagcgtc aagaccaact tggagacgcg
    18841 gcattcttgt caggtgttgc caaattccct caaagaatca agtgtgcaac cctagcttgg
    18901 aatgccctta agaaaacaat tgaaaatcaa gaaaaacagt aagacaagtt tcttttgtct
    18961 tatgaattat tagaaatgaa gaaaggatat tatggctgaa gaaagagtag aaccaaaacc
    19021 aattgacctt ggtgaatata aatttggttt ccatgacgat gtagagcctg tcttatcgac
    19081 aggaaaagga ctcaacgaag gtgttattcg tgaattatct gctgctaagg gtgagcctga
    19141 gtggatgttg gagttccgtt tgaagtctta tgaaaccttc aaaaaaatgc ccatgcaaac
    19201 ttggggagca gacttgtcag agattgactt tgatgactta atctactacc aaaaaccatc
    19261 tgacaaacca gcccgttctt gggatgatgt acctgaaaag attaaagaaa cctttgaacg
    19321 tatcgggatt ccagaagctg aacgtgctta tttagcaggg gcttctgccc agtacgagtc
    19381 agaagtggtt taccacaaca tgaaggaaga gttccaaaaa ttaggtatta tctttacaga
    19441 tacagattcc gcactcaagg aatacccaga cttatttaaa caatactttg cgaagttggt
    19501 accgccgaca gataacaagt tggcagccct caactcagca gtatggtcgg gtggaacttt
    19561 tatctacgtg ccaaaaggtg tcaaggtaga tattccactt caaacttatt tccgtatcaa
    19621 taacgaaaat ataggtcagt tcgaacgtac cttgattatc gttgatgagg gagcaagcgt
    19681 ccactacgta gaaggatgta cagcaccaac atattcaagc aatagcttgc acgctgccat
    19741 tgtagaaatt tttgctttgg acggagctta tatgcgttat acaactatcc aaaactggtc
    19801 tgataacgtc tataacttgg taacaaagcg tgctaaggct caaaaggatg ccactgttga
    19861 gtggattgat ggaaacttgg gtgccaaaac gactatgaaa tatccatctg tttatcttga
    19921 tggagaagga gcgcgtggta ccatgctctc tatcgccttt gctaatgcag ggcaacacca
    19981 agacacgggt gctaagatga ttcacaatgc tccacatacc agctcgtcta ttgtgtctaa
    20041 atccatcgct aaaggtggag gaaaggttga ctaccgtgga caagtcacct ttaacaagaa
    20101 ctctaagaaa tctgtttccc acattgaatg tgataccatt atcatggatg acttgtcagc
    20161 atcagatact attccattta atgaaattca caactcgcaa gtggctttgg aacacgaagc
    20221 caaagtatct aagatttcag aagagcaatt gtattatctc atgagccgtg gattgtcaga
    20281 atctgaggca actgaaatga ttgtcatggg atttgtagaa ccctttacaa aagaacttcc
    20341 aatggaatac gcagttgagc tgaaccgctt gattagctat gaaatggagg gatcagttgg
    20401 ataaaatttg attttatact cttcgaaaat ctcttcaaac cacgtcagca tcgccttacc
    20461 gtatgtatgg ttactgactt cgtcagtttc atctacaacc tcaaaacagt gttttgagca
    20521 acctgcggct agcttcctag tttattcttt gattttgagt attagattta ctcaaaatca
    20581 aggattttga agatgaactt gtatcaaaaa atcgcggttt aaaatcgcga ttttttataa
    20641 tttctcgtta acaaagcgga caaactgatt ccaccaaact tttaagaaga aggctttttc
    20701 aattttcttg tctgctacca tttcgaaact agggcgctct gtggtgatgt aaccttgacc
    20761 aatcaagtcc ttgtcttcat aagtcaaatg gccaaccact gttccagctt caagtggtgc
    20821 tgggattgct ttggaatcag gtgtgaattg aacagattgg gaagattgat tcccaacacg
    20881 ttcgattaga tagatatcct ctggagccac tgcagttact gtatcttctt ttccatcttg
    20941 tacaggggct ttgctatctt gataggcatc gccttgttga acgattttgc gaagtgtaaa
    21001 tgtagaagaa atataatcca ttagggaaga tgtagctgta aatcgagcgt aaggattatt
    21061 gtcttgatga tctgcattta aaacaactgt gatgactctc atgccttttt cgacagtagt
    21121 accaacaaaa gactctccag ccttatctgt tgttcctgtt tttagcccat caaaaccacc
    21181 acggtaagca ggcatacctt ctaacatgta gttggttgaa gtgattgtca tcccagcaaa
    21241 agtagaagaa ggttttttgg tgatttctaa gacttgtggg tattttttga tgaggttgcg
    21301 agcaacgata gcgacatcat aagcactaag cttattttcc tcatcttttt tagaacctgg
    21361 gtaaatgtta tcccctagag tttcattgtt aagacctgtc gtattgacaa cagtggcatc
    21421 ctgaattccc cattccaaga gttttgcccg catcatatcg atgaaatctt tttctgagcc
    21481 agcaattttc tcagctaggg caatagcggc gctgttggca ctagatacca gagttgcttc
    21541 aagcaactct tcgacagtat aattacgggc ctccatagga atattactgg cttcagaatt
    21601 tgtcgtcaat tgataaggat aatcagaaat atctacagga gtggagaggg taatacttcc
    21661 gttttccaaa gcttcataga ccagataagc agtaatcaat tttgttatgg aagcaatttc
    21721 gacaggttgc gttgcatcct tctcatagag aattttacca gtatttgcct caacagcaat
    21781 cgcatgttta gcggcaatgg taaaatcttg agcaacagca gtagaagcac cccctaaaag
    21841 agagacagtt aacaaagtta aaaatatttt tttcatagta gtcttattct atcataaaga
    21901 aaaaaaatat tcttgcttta ataattcatc tgttaagctt tttgaaaata tggtaaaata
    21961 aagtgaggga ggtaactcat gtttcgtaga aataaattat ttttttggac cacagaaatt
    22021 ttactcttaa ccatcatctt ttacctatgg agacagatgg ggtctttgat taaccctttt
    22081 gttagcgtgc ttaatacaat tatgattcca tttttattag ggggctttct ttattatttg
    22141 acaaacccta ttgttacttt cttaaataaa gtctgtaaac tcaatcgttt gcttggtatt
    22201 ttaattacct tgtgtacttt ggtctgggga atggtcatag gtgttgtcta tctcttacct
    22261 attttgatta atcagttatc tagtttgatt atatctagtc aaactattta tagtcgagta
    22321 caagacttaa tcatagactt atctaattat cctgcgctcc agaatttgga tgtagaagct
    22381 acaattcagc agttaaactt atcctatgtt gatattcttc aaaatatcct aaatagcgta
    22441 tcaaatagtg tggggagcgt cttgtcagct cttatcagta ctgttttgat tttgattatg
    22501 actccagttt ttttggttta tttcttatta gatggacata aattcttgcc catgcttgaa
    22561 agaacgattc taaagaggga tcgcttgcat attgcaggct tattaaagaa tttaaatgcg
    22621 acgattgctc gctatattag tggagtttcg attgacgcaa tcattatagg ttgtttggct
    22681 tatattggct atagtattat tggtttaaaa tatgctttag tttttgccat tttttctggt
    22741 gtagccaatt taattcctta tgtggggcca agtattggtt tgattcctat gatcatcgca
    22801 aatatattca ctgatcccca tagactgctg attgcagtga tttatatgct tgttgttcag
    22861 caggtagatg gcaatatctt atatcctcga atcgtaggaa gtgttatgaa ggttcatcca
    22921 atcacgattt tagttttact tttgttgtca agcaatatct atggtgtagt tggaatgatt
    22981 gtcgcagtgc caacctattc tatcttgaaa gaaatttcta agttcttatc ccgtttgtat
    23041 gaaaatcata aaataatgaa agaacgagaa agagaattag ctaagtaaaa gtcaggagaa
    23101 ccctgatttt tctttactgg aagtggcctt tagattagaa gactgaaaat aagttaaagt
    23161 cttaaactaa ttttcacagc taagaatagt agaagttaat ctgataaaaa tcgaaaaaac
    23221 cagtggaatt ctgtgtcagg gtaagttcca ctggttttca tagtctatta aagttcgaat
    23281 gaaacctatt tatagtagat tgaaactaga atagtacacc tctaattcta aaacattgtt
    23341 agaaatcgat ttgactgtcc tgatctattc gttctattct tatttcattt tactatattt
    23401 tggtgcaata agtgaaaagt agtccgaata atataaggat tgattttata gtttttaaac
    23461 tcaaatgaat tgaaataaag agagtacgaa aattctcatc tgaaagtatt ttagaataat
    23521 tctcttcgtg aatttcttca aaacagatag cttcatctta ggtatgtgat ttctttttgc
    23581 atttttgagt tagataaggt ataatggttt tattgtcttt tggggtcgtt acggattcga
    23641 caggcattat gaggcatatt ttgcgactcg tgtggcgacg taaacgctca gttaaatata
    23701 actgcaaaaa ataacacttc ttacgctcta gctgcctaaa aaccagcagg cgtgacccga
    23761 tttggattgc tcgtgttcaa tgacaggtct tattattagc gagatacgat taagccttgt
    23821 ctagcggttt gataagagat tgatagactc gcagtttcta gacttgagtt atgtgtcgag
    23881 gggctgttaa aataatacat aacctatggt tgtagacaaa tatgttggca ggtgtttgga
    23941 cgtgggttcg actcccaccg gctccattat tcctttgcat tcttttgcta aaaagggagt
    24001 cacaaacaga ccctatttta aaaaaaggat agaaaaaagg atacaacatt tgtcgcatcc
    24061 taaaaataat cttttttcca cggaagacat gggattcgaa cccacgcacg ctattacacg
    24121 cctaccgcgt ttccaacacg gcctcttaag cctcttgagt aatcttccaa tacttactca
    24181 aatagtctac cataaaggct cttatcttgc aataaaaatt ctagaaataa gaaaaatgat
    24241 agattttgaa agaaaatgat aaaaaatgct tgacttcaaa agaaagtatg atagaatgaa
    24301 tagtgtaaac gataacagga ggtgattcgg tgttaaaaac agagcggaag caactgattt
    24361 tagaggagtt aaatcaacat catgtagttt ctctagaaaa attagttagt ttgctagaaa
    24421 cgtcagaatc aacggttcga agagacttgg atgagttgga agcggaaaac aagcttcgtc
    24481 gtgtgcatgg tggagcagaa ctcccccact ccttacagga agaagaaacc attcaagaaa
    24541 aatctgtcaa aaaccttcaa gaaaagaagt tgctggctca gaaagcagcc tctctcatta
    24601 aagaaaaaga tgtcatcttt atcgatgctg gaacaacaac tgcttttttg attcatgaat
    24661 tggtcaataa gaatattaca gttgtgacca actccattca ccatgccgct cagttggttg
    24721 aaaagcagat tccaactgtc atggttggag gaaacgtcaa gacggcgaca gatgctagta
    24781 tcggtggcgt tgctcttaac cagattaacc aattgcactt tgaccgtgcc tttatcggaa
    24841 tgaatggtgt tgacgatggc tattatacga ctcctgatat ggaggaggga gctgtgaaaa
    24901 gagctatttt ggagaatgcc aagcagacct acgtcttggt ggattcgtca aaaattggac
    24961 aaacttgctt tgccaaggta gcaccactca aacgcgctat cgttatcact agtcaagggc
    25021 atgagctctt gcaggttatt aaggagaaaa cggaggtaat agaagtatga tttatacagt
    25081 cacactcaat ccatccattg actatatcgt tcgtttggac caagtcaaag ttggtagtgt
    25141 aaatcgtatg gacagtgatg ataagtttgc tggtgggaaa ggaatcaatg tcagccgtgt
    25201 cttgaaacgt ttgaatatac caaatacagc gacgggattt atcggtggct ttactggtaa
    25261 atttatcaca gatactttag cagaggaaga aatcgagaca cgttttgtcc aggtggcaga
    25321 agatactcgt atcaatgtta aaatcaaagc agaccaagaa acagaaatca acggaacggg
    25381 tccaactgtt gaatcggttc agctagaaga attgaaagct attttatcta gtctgacagc
    25441 agaagataca gttgtctttg caggttcaag tgctaaaaat ctaggcaatg ttatctataa
    25501 ggatttgatt tccttgacgc gccagactgg tgcgcaagtg gtctgtgact ttgaaggaca
    25561 gaccttaatt gatagtttgg actaccagcc tcttcttgta aaaccaaaca atcatgaact
    25621 tggagcgatt tttggggtta aactcgaaag tttagatgaa attgagaaat acgctcgtga
    25681 gttactggct aagggtgctc aaaatgttat tatctctatg gctggtgatg gtgcccttct
    25741 tgtcacatct gagggagctt acttcgctaa accaatcaaa ggaacagtca aaaattcagt
    25801 tggagctggt gattctatgg ttgctggatt cacaggtgaa tttgtcaaat caaaagacgt
    25861 agtagaagcc ttcaaatggg gagtggcttg cggaacggca actaccttct cagatgactt
    25921 ggcaacggcg gaatttatta aagaaacata tggaaaagtt gaggtagaaa aacgatgaaa
    25981 attcaagacc tattgagaaa agatgtcatg ttgctagatt tgcaggcaac tgaaaaaaca
    26041 gctgtcatcg acgagatgat taaaaatttg acagaccacg gttatgtaac agattttgaa
    26101 acatttaaag aaggaatttt ggcgcgtgaa gctttgactt ctactggttt gggtgatgga
    26161 atcgcaatgc ctcacagcaa aaacgctgct gtcaaagaag cgacagttct atttgctaag
    26221 tcaaataagg gtgttgacta cgagagcttg gatggacaag caactgacct cttcttcatg
    26281 attgcagctc cagaaggtgc caatgatact cacttggcag ccttggcaga attgtctcaa
    26341 tacttgatga aagacggttt tgcagacaaa cttcgtcaag caacatctgc agaccaagtt
    26401 atcgaacttt ttgaccaagc ttcagaaaaa actgaggaac ttgttcaagc acctgctaat
    26461 gactctggtg actttatcgt agctgttaca gcttgtacaa caggtattgc ccacacttac
    26521 atggcccaag aagcccttca aaaagtagct gctgaaatgg gggttggtat caaggtcgaa
    26581 accaacggtg ctagcggtgt tggaaatcaa ctaactgcag aagatatccg taaggctaaa
    26641 gctattatca ttgcagcaga caaggccgtt gaaatggatc gatttgatgg aaaaccattg
    26701 atcaatcgtc cagttgctga cggtatccgt aagacagaag agctaattaa cttggctctt
    26761 tcaggagata ctgaagtcta ccgtgccgct aatggtgcca aagctgcaac agcctctaac
    26821 gaaaaacaaa gccttggtgg tgccttgtac aaacacttga tgagtggtgt atctcaaatg
    26881 ttaccattcg ttatcggtgg tggtatcatg attgcccttg ccttcttgat tgacggtgct
    26941 ttgggtgttc caaatgaaaa ccttggcaat cttggttctt accatgagtt agcttctatg
    27001 ttcatgaaaa ttggtggagc tgcctttggt ttgatgcttc cagtctttgc gggttatgtt
    27061 gcctactcta ttgctgaaaa accgggtttg gtagcaggtt tcgtggctgg tgctattgcc
    27121 aaagaaggtt ttgcctttgg taaaattcct tatgccgcag gtggtgaagc aacttcaact
    27181 cttgcaggtg tctcatctgg tttcctaggt gcccttgttg gtggatttat cgcaggtgcc
    27241 ttggttcttg ccatcaagaa atacgttaaa gttcctcgtt cactcgaagg tgctaaatca
    27301 atccttctat tgccacttct tggaacaatc ttgacaggat ttgttatgct agctgtgaat
    27361 atcccaatgg ctgcaatcaa cactgctatg aatgacttcc taggcggtct tggaggaggt
    27421 tcagctgtcc ttcttggtat cgtccttggt ggaatgatgg ctgttgacat gggtggacca
    27481 gttaataaag cagcttatgt ctttggtaca ggtacgcttg cagcaactgt ttcttcaggt
    27541 ggttctgtag ccatggcagc agttatggct ggaggaatgg tgccaccact tgcaatcttt
    27601 gtcgcaactc ttcttttcaa agataaattt actaaggaag aacgtaactc tggtttgaca
    27661 aacatcatca tgggcttgtc atttatcact gagggagcga ttccatttgg tgccgctgac
    27721 ccagctcgtg cgattccaag cttcatcctt ggttcagcag tagcaggtgg actcgttggt
    27781 cttactggta tcaaactcat ggcgccacac ggaggaatct tcgttatcgc ccttacttca
    27841 aatgctctcc tttacctcgt ttctgtcttg gtaggagcaa tcgtaagtgg tgtggtttat
    27901 ggttacctac gcaaaccaca agcataaaaa atagaaaaat gaaaagattg gaccgtttgg
    27961 tgcagtcttt ttctcttccc gaaatgcctg tgaaatatgg tataatagaa gaatggcaaa
    28021 caagaataca agtacaacaa gacggagacc gtctaaagca gaactggaaa gaaaagaagc
    28081 gattcaacga atgttgattt cgttaggaat tgcgatttta ttgattttcg cagccttcaa
    28141 attaggggct gcaggtataa ccctttataa tttaattcgc ttgctagtgg gtagcctagc
    28201 ttatctggcg atattcggcc tattaatcta tctcttcttt ttcaagtgga tacgaaaaca
    28261 ggaaggactc ttatctggct ttttcaccat atttgctggc ttactcttga tttttgaggc
    28321 ctacttggtt tggaaatatg gtttggacaa gtccgttcta aaagggacca tggctcaggt
    28381 tgtgacagat ctgactggtt ttcgaacgac tagctttgct ggagggggct tgatcggggt
    28441 cgctctttat attccaacag cctttctctt ttcaaatatc ggaacttact ttattggttc
    28501 tatcttgatt ttagtgggtt ctctcctagt cagcccttgg tctgtttacg atattgctga
    28561 atttttcagt agaggctttg ccaaatggtg ggaagggcac gagcgtcgaa aagaggaacg
    28621 ctttgtcaaa caagaagaaa aagctcgcca aaaggctgag aaagaggcta gattagaaca
    28681 agaagagact gaaaaagcct tactcgattt gcctcctgtt gatatggaaa cgggtgaaat
    28741 tctgacagag gaagctgttc aaaatcttcc acctattcca gaagaaaagt gggtggaacc
    28801 agaaatcatc ctgcctcaag ctgaacttaa attccctgaa caggaagatg actcagatga
    28861 cgaagatgtt caggtcgatt tttcagccaa agaagccctt gaatacaaac ttccaagctt
    28921 acaactcttt gcaccagata aaccaaaaga tcagtctaaa gagaagaaaa ttgtcagaga
    28981 aaatatcaaa atcttagaag caacctttgc tagctttggt attaaggtaa cagttgaacg
    29041 ggccgaaatt gggccatcag tgaccaagta tgaagtcaag ccggctgttg gtgtaagggt
    29101 caaccgcatt tccaatctat cagatgacct cgctctagcc ttggctgcca aagatgtccg
    29161 gattgaagca ccaatccctg ggaaatccct aatcggaatt gaagtgccca actccgatat
    29221 tgccactgta tctttccgag aactatggga acaatcgcaa acgaaagcag aaaatttctt
    29281 ggaaattcct ttagggaagg ctgttaatgg aaccgcaaga gcttttgacc tttctaaaat
    29341 gccccacttg ctagttgcag gttcaacggg ttcagggaag tcagtagcag ttaacggcat
    29401 tattgctagc attctcatga aggcgagacc agatcaagtt aaatttatga tggtcgatcc
    29461 caagatggtt gagttatctg tttacaatga tattccccac ctcttgattc cagtcgtgac
    29521 caatccacgc aaagccagca aggctctgca aaaggttgtg gatgaaatgg aaaaccgtta
    29581 tgaactcttt gccaaggtgg gagttcggaa tattgcaggt tttaatgcca aggtagaaga
    29641 gttcaattcc cagtctgagt acaagcaaat tccgctacca ttcattgtcg tgattgtgga
    29701 tgagttggct gacctcatga tggtggccag caaggaagtg gaagatgcta tcatccgtct
    29761 tgggcagaag gcgcgtgctg caggtatcca catgattctt gcaactcagc gtccatctgt
    29821 tgatgtcatc tctggtttga ttaaggccaa tgttccatct cgtgtagcat ttgcggtttc
    29881 atcaggaaca gactcccgta cgattttgga tgaaaatgga gcagaaaaac ttcttggtcg
    29941 aggagacatg ctctttaaac cgattgatga aaatcatcca gttcgtctcc aaggctcctt
    30001 tatctcggat gacgatgttg agcgcattgt gaacttcatc aagactcagg cagatgcaga
    30061 ctacgatgag agttttgatc caggtgaggt ttctgaaaat gaaggagaat tttcggatgg
    30121 agatgctggt ggtgatccgc tttttgaaga agctaagtct ttggttatcg aaacacagaa
    30181 agccagtgcg tctatgattc agcgtcgttt atcagttgga tttaaccgtg cgacccgtct
    30241 catggaagaa ctggagatag caggtgtcat cggtccagct gaaggtacca aacctcgaaa
    30301 agtgttacaa caataaaaaa atagcttctt tccaagtttg gagggaagct attttagtgg
    30361 ctattgattg cttttatttt ctgaagttgg cgcattggac tgtttttcgt tttcagtagc
    30421 aggtttactt gaagcaggag tagaagagtc ctgagttgct gttttctgat cttctttttt
    30481 ctcttccttg acgctagatt ttggtgtttc ctcttgctgt gttttttctt gactagtgtt
    30541 agtctcttta gttggactgg tgttttcctt aggggattcc ttttggattt ctttgacaat
    30601 ggttgtcgtc tggcttgtcg taggttcttt tttaatattt ttgttattat ccaaggcgtt
    30661 cataatcgtg atacttgcgg taatcaagcc aaggatacta gcgattgtag caacggtttt
    30721 ttgaaagact gtaaagcctt ttttgatgtg ttctgttttt ggttttgaag ttctacgata
    30781 gttagacata cattctctcc tttgattatg atttattata ctctgtttct ttaaaaaaat
    30841 cttaaaaaaa gagaaatttc cctttcttgt cgcaagctcc gtttcatgat acaatagagg
    30901 aagtgatttt agaaagcgtg agaaacatga tttactttga taattcagcg acgaccaagc
    30961 cttatcctga agcccttgaa acctatatgc aggtcgcttc aaaaatttta ggaaacccat
    31021 ctagtctcca tcgtttggga gaccaagcaa cacgaatctt agatgcttct cgccaacaga
    31081 ttgcagattt aatcggtaag aaaagcgatg aaatcttctt tacctcgggt ggaacagaag
    31141 gggataactg gcttatcaag ggtgtggcct ttgaaaaagc tcagtttggc aagcacatca
    31201 ttgtttcagc cattgaacat ccagcagtca aagagtcagc cctctggttg aaaagtcaag
    31261 gatttgaagt ggattttgct ccagttgata agaaaggctt ggtcgatgtt gaggcgttag
    31321 caggtttgat acggcatgat acaatcctcg tttccatcat ggctgtgaac aatgaaatcg
    31381 gctctatcca acctattgag gctatttcag aattcttggc agacaagccg actatttcct
    31441 tccacgttga tgcggttcag gcgcttgcca aaattccgac tgaaaagtat ctgacagaac
    31501 gggtggattg cgcgactttc tctagtcaca agttccacgg ggttcgaggt gttggctttg
    31561 tctatatcaa atctggcaag aagattacac ctcttcttac aggtggtggc caggagcgag
    31621 attatcgttc gacaactgaa aatgtggcag ggattgcagc gacagccaag gccctccgtt
    31681 tgtctatgga aaagctagat atctttagga gcaagactgg gcagatgaag gcagtgattc
    31741 gccaagctct tctgaactat ccggatattt ttgtcttttc agatgaggaa aactttgcac
    31801 ctcatattct gacttttgga atcaaaggtg ttcgaggtga agtcatcgtt cacgcctttg
    31861 aagactatga tattttcatc tcaacaacct cagcttgttc atctaaggca ggaaaaccag
    31921 ccggtacctt gattgccatg ggagtggaca aagataaggc caagtcagct gtgcgtctta
    31981 gcctagactt ggaaaatgat atgagtcagg tcgagcagtt tttgaccaag ttaaaattga
    32041 tttacaatca aactagaaaa gtaagatagg agcattcatg cagtattcag aaattatgat
    32101 tcgctacgga gagttgtcaa ccaagggtaa aaaccgtatg cgtttcatca ataaacttcg
    32161 taataatatt tcggacgttt tgtctatcta tacccaagtt aaggtaacag cagatcgcga
    32221 ccgtgcccac gcttacctca atggagctga ttacacagca gttgcagaat ctctcaaaca
    32281 agtttttgga attcaaaact tttctcctgt ttataaggtt gaaaaatctg tagaagtttt
    32341 gaagtcttct gtccaagaga ttatgcggga catctacaag gaaggtatga cctttaagat
    32401 ttctagcaag cgtagcgacc acaactttga acttgatagt cgtgaactca accaaacact
    32461 tggaggggct gtattcgaag ccattccaaa tgtgcaagtt caaatgaaaa gtcctgacat
    32521 caatcttcag gtggagattc gtgaagaagc agcctatctt tcttatgaaa ccattcgtgg
    32581 ggctggtggt ttgccagttg gaacttcagg taaagggatg ctcatgttgt caggagggat
    32641 tgactcacct gtagcaggtt atcttgctct taagcgtggg gtggatatcg aggcagttca
    32701 ctttgctagt ccaccatata ctagtcctgg tgccctcaag aaagcgcagg acttgacccg
    32761 taaattgacc aagtttggcg gaaatatcca gtttatagag gtgcctttca cagagattca
    32821 agaggaaatc aaagccaaag cgccagaagc ttatttgatg actctaactc gtcgctttat
    32881 gatgcggatt actgaccgta ttcgtgaggt acgaaatggt ttggttatca tcaatgggga
    32941 aagtctaggt caagtagcca gccaaaccct tgaaagtatg aaggctatca atgctgttac
    33001 caacactccc atcattcgtc ctgtggttac catggacaag ttggaaatca ttgacatcgc
    33061 ccaggaaatc gatacctttg acatttcaat ccaaccgttt gaagactgtt gtaccatttt
    33121 tgcaccagat cgtccaaaaa caaatcctaa aattaagaat gcggagcagt acgaagcgcg
    33181 tatggatgtt gaaggcttgg ttgagcgagc agtggctgga atcatgatta ctgaaatcac
    33241 acctcaagcc gaaaaagatg aagttgatga cttgattgac aatctgctct aattcagaaa
    33301 atccaaaaga atagcgaaaa tcagtaaaaa aagttagttt tttctctaaa aacaggtaaa
    33361 aaactaactt tttttatttt tatgatataa tgatataaaa ttttgaatat agagagtttt
    33421 ctgacaatga atcaatccta cttttatcta aaaatgaaag aacacaaact caaggttcct
    33481 tatacaggta aggagcgccg tgtacgtatt cttcttccta aagattatga gaaagataca
    33541 gaccgttcct atcctgttgt atactttcat gacgggcaaa atgtttttaa tagcaaagag
    33601 tctttcattg gacattcatg gaagattatc ccagctatca aacgaaatcc ggatatcagt
    33661 cgcatgattg tcgttgctat tgacaatgat ggtatggggc ggatgaatga gtatgcggct
    33721 tggaagttcc aagaatctcc tatcccaggg cagcagtttg gtggtaaggg tgtggagtat
    33781 gctgagtttg tcatggaggt ggtcaagcct tttatcgatg agacctatcg tacaaaagca
    33841 gactgccagc atacggctat gattggttcc tcactaggag gcaatattac ccagtttatc
    33901 ggtttggaat accaagacca aattggttgc ttgggcgttt tttcatctgc aaactggctc
    33961 caccaagaag cctttaaccg ctatttcgag tgccagaaac tatcgcctga ccagcgcatc
    34021 ttcatctatg taggaacaga agaagcagat gatacagaca agaccttgat ggatggcaat
    34081 atcaaacaag cctatatcga ctcgtcgctt tgctattacc atgatttgat agcaggggga
    34141 gtacatctgg ataatcttgt gctaaaagtt cagtctggtg ccatccatag tgaaatccct
    34201 tggtcagaaa atctaccaga ttgtctgaga ttttttgcag aaaaatggta agttaagaaa
    34261 ggaaaaaacg aaatgcatat tgaacatctt agccactgga gtggtcatct taaccgtgaa
    34321 atgtacctta accgttatgg acatggtggg attccagttg tggtctttgc ttcatcaggt
    34381 ggtagtcaca acgaatacta tgattttggc atgattgatg cctgtgcttc ctttatcgag
    34441 gaaggccttg tccagttctt taccctatct agtttggata gtgagagctg gttggctact
    34501 tggaaaaatg ctcatgacca agcggaaatg caccgtgcct acgaacgtta tgtgattgag
    34561 gaggccattc ttttatcaag cacaagacag gttggtttga tggcatgatg acgacaggtt
    34621 gctctatggg agcctatcat gcactcaatt tcttcctcca gcatccagat gtctttacca
    34681 aagtgattgc tctcagtggt gtttacgacg cacgtttctt tgtcggtgat tactacaacg
    34741 atgatgctat ttaccaaaac tcgccagtag attatatttg gaaccaaaac gacggctggt
    34801 ttattgaccg ttaccgtcag gcagagattg tgctgtgtac ggggcttgga gcctgggaac
    34861 aagatggttt gccatccttt tacaagctca aagaagcctt tgacaagaaa caaattccag
    34921 cctggtttgc tgaatgggga catgatgtcg cccatgactg ggaatggtgg cgtaaacaaa
    34981 tgccttattt cctcggtaat ctctatttat aaaaggagtt acctatgaat taccttgtta
    35041 tttctcccta ctatccacaa aactttcaac agtttaccat cgaactagct aataaaggca
    35101 tcacagtctt gggaattggt caagagtctt acgagcaatt ggatgagccc ttgcgcaata
    35161 gcttgaccga gtattttcgt gttgataatc ttgagaacat agatgaagtc aaacgtgcag
    35221 ttgcttttct cttttataaa catggtccaa ttggccgcat cgagtctcac aatgaatact
    35281 ggcttgagct agacgcaaca ctcagagaac aattcaatgt ttttggtgcc aaaccagagg
    35341 atctcaaaaa gacgaaatat aagtctgaaa tgaagaaact tttcaaaaaa gcaggtgttc
    35401 ctgtggtacc tggagctgtt atcaagacgg aagcagatgt tgatcaagca gtgaaagaaa
    35461 tcggtcttcc aatgattgcc aaacctgata atggagtggg agcagccgca acctttaaac
    35521 ttgagacaga agacgatatc aatcacttca agcaagaatg ggaccattca accctttatt
    35581 tctttgaaaa atttgtcact tccagcgaaa tctgtacctt tgacgggctc gtggacaagg
    35641 atggaaagat tgtcttctca acaacctttg actacgccta tacaccgctt gacctcatga
    35701 tttataagat ggacaattct tattatgtgc tcaaggatat ggatcctaaa ctgcgcaagt
    35761 atggggaagc aattgtcaaa gaatttggta tgaaagaacg gtttttccat attgagttct
    35821 tccgtgaggg ggacgattat attaccatcg agtacaataa ccgccctgca ggtggtttta
    35881 ccattgatgt ttataacttt gctcattcct tggaccttta tcgtggctat gcagctattg
    35941 tcgcaggaga ggagttcccg gcgtcagact ttgaaactca gtattgtttg gctacttctc
    36001 gccgtgcaaa tgctcactat gtttattcag aagaggattt gcttgccaaa tatagccagc
    36061 agttcaaggt taaaaaagtc atgccagctg ccttcgcgga acttcaagga gattacctgt
    36121 atatgctgac cactccgagt cgacaagaaa tggagcagat gattgcagat ttcggacaac
    36181 gtcaagaata agaactatcg gattaaggaa attaactccc ttaatccttt tgttttgtct
    36241 gataaaaaat aagagcatcc caacaaggta gctatcataa aacttgttcg ataactattt
    36301 gaagcaggat taggtggtca gaaattaaat tttaatattt caattgagtc atagtattgt
    36361 gtttgcgtat ccttaaatca gctaaaagga tccatgacga cacctatacg atatagtttt
    36421 caagatacca aacaagtcta ttaatattca atgaaaatca aagagcaaac taggaagcta
    36481 gccgcaggtt
//
LOCUS       DAVBCV010000015        35153 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBCV010000015 DAVBCV010000000
VERSION     DAVBCV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35153
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.015"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            236..574
                     /locus_tag="VQ516_000741"
     CDS             236..574
                     /locus_tag="VQ516_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2511216.1"
                     /translation="MTLTIHVYYKGDGDCAIKFAREMLESGLVAEIRCQKGNLKYEYF
                     LPIEKEGIILLIDQWINQEALDEHHQSNTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            764..2200
                     /locus_tag="VQ516_000742"
     CDS             764..2200
                     /locus_tag="VQ516_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2511217.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKKVILSNGESVE"
     gene            2252..2491
                     /locus_tag="VQ516_000743"
     CDS             2252..2491
                     /locus_tag="VQ516_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2511218.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2621..2935
                     /locus_tag="VQ516_000744"
     CDS             2621..2935
                     /locus_tag="VQ516_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2511219.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            3053..5032
                     /locus_tag="VQ516_000745"
     CDS             3053..5032
                     /locus_tag="VQ516_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2511220.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFISIFEKIK"
     gene            5042..5356
                     /locus_tag="VQ516_000746"
     CDS             5042..5356
                     /locus_tag="VQ516_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2511221.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5387..5893
                     /locus_tag="VQ516_000747"
     CDS             5387..5893
                     /locus_tag="VQ516_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511222.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5973..7319
                     /gene="celB"
                     /locus_tag="VQ516_000748"
     CDS             5973..7319
                     /gene="celB"
                     /locus_tag="VQ516_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2511223.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7699..7875
                     /locus_tag="VQ516_000749"
     CDS             7699..7875
                     /locus_tag="VQ516_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511224.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8285..10498
                     /locus_tag="VQ516_000750"
     CDS             8285..10498
                     /locus_tag="VQ516_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HET2511225.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHFFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10720..11196)
                     /locus_tag="VQ516_000751"
     CDS             complement(10720..11196)
                     /locus_tag="VQ516_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2511226.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11419..14619
                     /locus_tag="VQ516_000752"
     CDS             11419..14619
                     /locus_tag="VQ516_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2511227.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESDKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDYQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYML
                     IPNVGRATFNQMIKELERSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            14999..15384
                     /gene="tnpA"
                     /locus_tag="VQ516_000753"
                     /pseudo
     CDS             14999..15384
                     /gene="tnpA"
                     /locus_tag="VQ516_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15763..16392)
                     /locus_tag="VQ516_000754"
     CDS             complement(15763..16392)
                     /locus_tag="VQ516_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2511228.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(16402..17403)
                     /locus_tag="VQ516_000755"
     CDS             complement(16402..17403)
                     /locus_tag="VQ516_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2511229.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(17434..18075)
                     /locus_tag="VQ516_000756"
     CDS             complement(17434..18075)
                     /locus_tag="VQ516_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2511230.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(18094..18909)
                     /locus_tag="VQ516_000757"
     CDS             complement(18094..18909)
                     /locus_tag="VQ516_000757"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2511231.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            19180..19608
                     /locus_tag="VQ516_000758"
     CDS             19180..19608
                     /locus_tag="VQ516_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2511232.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPVKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEDFESGI"
     gene            19620..20810
                     /locus_tag="VQ516_000759"
     CDS             19620..20810
                     /locus_tag="VQ516_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2511233.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            20821..21312
                     /locus_tag="VQ516_000760"
     CDS             20821..21312
                     /locus_tag="VQ516_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2511234.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLACNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            21327..22106
                     /locus_tag="VQ516_000761"
     CDS             21327..22106
                     /locus_tag="VQ516_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2511235.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTANHVVEEEAHDDWI"
     gene            22093..22911
                     /locus_tag="VQ516_000762"
     CDS             22093..22911
                     /locus_tag="VQ516_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2511236.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVVIGIIGSWLKIIA"
     gene            22911..23204
                     /locus_tag="VQ516_000763"
     CDS             22911..23204
                     /locus_tag="VQ516_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2511237.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQTEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            23226..25127
                     /locus_tag="VQ516_000764"
     CDS             23226..25127
                     /locus_tag="VQ516_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2511238.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDMLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSNKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            25187..26188
                     /locus_tag="VQ516_000765"
     CDS             25187..26188
                     /locus_tag="VQ516_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2511239.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(26298..26633)
                     /locus_tag="VQ516_000766"
     CDS             complement(26298..26633)
                     /locus_tag="VQ516_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511240.1"
                     /translation="MKSLARLLNIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(26644..26886)
                     /locus_tag="VQ516_000767"
     CDS             complement(26644..26886)
                     /locus_tag="VQ516_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511241.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(26897..27439)
                     /locus_tag="VQ516_000768"
     CDS             complement(26897..27439)
                     /locus_tag="VQ516_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2511242.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(27455..27649)
                     /locus_tag="VQ516_000769"
     CDS             complement(27455..27649)
                     /locus_tag="VQ516_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2511243.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            27815..28765
                     /gene="rsmH"
                     /locus_tag="VQ516_000770"
     CDS             27815..28765
                     /gene="rsmH"
                     /locus_tag="VQ516_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2511244.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            28777..29094
                     /gene="ftsL"
                     /locus_tag="VQ516_000771"
     CDS             28777..29094
                     /gene="ftsL"
                     /locus_tag="VQ516_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2511245.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            29098..31350
                     /gene="pbpX"
                     /locus_tag="VQ516_000772"
     CDS             29098..31350
                     /gene="pbpX"
                     /locus_tag="VQ516_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2511246.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDSFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAVYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            31352..32332
                     /locus_tag="VQ516_000773"
     CDS             31352..32332
                     /locus_tag="VQ516_000773"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2511247.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNMGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLSFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            32941..35046
                     /locus_tag="VQ516_000774"
     CDS             32941..35046
                     /locus_tag="VQ516_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2511248.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQEKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVVIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
BASE COUNT        11084 a         5823 c         7594 g        10652 t
ORIGIN      
        1 tcaaaaagca cctcaaaaag gtattaccaa gttgcaatac cttttatgag gctcttttgt
       61 cttgttcttg tttcaattga ctataaatcg ctataagaaa aaatcatttt attggtataa
      121 agaagtgatt gaaagtaatg gcgaaacgtt agataattag ttcataaaaa acaagtttag
      181 atcctcatct aaacttgttt ttttaaaatt gaaggatgat tttggaggat atgagatgac
      241 attgactatt catgtatatt ataaaggcga tggagattgt gcaataaagt ttgcaagaga
      301 aatgcttgaa tctgggcttg tcgcagaaat tagatgtcaa aaaggtaatt tgaaatacga
      361 atatttttta ccaattgaaa aagagggaat tattctcctt attgatcagt ggataaatca
      421 agaagcttta gatgaacatc atcaatcaaa tacaatgcag aaaatcttag atttaagaaa
      481 aaaatatcac ttgcagatgc aagttgaacg ttatatagaa gatgattctg ggatgccaga
      541 aagcgataag tcgtttatag acacagggaa ttagaattat tgtgagagtt tttgtagttt
      601 gaaagtattg aaaatcgtaa agatatcatt acgagaatac aagaagatat tgttctattt
      661 ccattaaaaa ttacttcttt ccttaataat aaggaaaaag aatacgtttt catcttttgg
      721 tttgaggttt ataataagaa tatcaaagtc aggaggaaaa aagatgacta cttttaaaga
      781 tggattttta tggggtggtg ctgttgctgc tcatcaactt gaaggcggat ggcaagaagg
      841 tggcaaagga attagtgttg ctgatgttat gactgctggt cgccatggag tagctcgtga
      901 aattactttg ggagttttag agggtaaata ttatccaaat catgaggcga tagattttta
      961 tcatcgttat aaagaagaca tagcactttt tgctgaaatg ggattcaagt gcttccgtac
     1021 ctctattgca tggacacgta tctttccaaa aggtgatgag ttagagccga atgaagaagg
     1081 attacagttt tatgataatc tttttgatga atgcttaaag aatggtattg aacctgtcat
     1141 cactctatct cattttgaaa tgccttatca cttagtgacc gaatatggtg gttggaaaaa
     1201 taggaaattg attgatttct ttgctcgttt tgcagaagtc gtatttaaac gttacaaaga
     1261 taaggttaaa tattggatga ctttcaatga aatcaataat caagcgaatt atcaggaaga
     1321 ttttgcacca tttactaact caggtattgt atatgaggaa ggtgataata gagaagcaat
     1381 tatgtatcaa gcagcacatt acgaattagt tgcttctgca cgagctgtaa aaattggtca
     1441 tgagattaat ccagattttc aaataggttg tatgattgcg atgtgtccaa tttatccagt
     1501 tacttgcaat cctaaggata tcttaatggc aatgaaagct atgcagaagc gttattattt
     1561 tgctgatgtg catgttttag gtaaatatcc tgagcatatt ttcaagtatt gggaacgaaa
     1621 aggtatttca gttgatttta ctgaccagga taaagaagat ttacttggtg ggactgtaga
     1681 ttacattggt ttcagttact atatgtcctt tgctatagac tctcatcgtg aaaataatcc
     1741 ttattttgat tatcttgaaa cagaagattt agtgaaaaat aattatgtta aggcttctga
     1801 atgggagtgg caaattgatc cagaaggttt acgttatgcg ttaaattggt ttacagacca
     1861 ctatcactta ccactcttta ttgttgaaaa tggttttgga gctatagatc aagttgcagc
     1921 agatggtatg gtacatgatg attatagaat tgaatatcta ggtgcccata ttcgtgaaat
     1981 gaaaaaggct gtagttgaag atggtgttga tttaatgggt tatactccat ggggatgtat
     2041 tgatttggtt tcagctggta ccggtgaaat gcggaaacgt tatggcttta tttatgtaga
     2101 taaagatgac aatgggaagg gaagttataa tcgttccccg aaaaaatctt ttggctggta
     2161 taagaaagtt attttatcta acggtgaatc agtagaatag tataaacaaa ctgagattat
     2221 tctcagtttg tttagcatat tgaggagaca tatgattgga agtattgcag caattttaac
     2281 aacatttgca tttttaccac aagtttttcg tgtagttaag acaaaggaca caggatctat
     2341 tgctctaggt atgtatgtta tgcaagttat tggtattgca ttgtggcttg ctcatggtat
     2401 tcgtatagga gacttgcctt tgattttagc aaatagtgtt tcatttctac tctcaggaat
     2461 aattttattt tataaattga agtataaata atttttttcc atatcattta ggaaaatgag
     2521 ggaattgtca ttgaaaacgc tttattttat aataaaaata gataacaaat aagccaactt
     2581 agcacagctt tgattggatt taagaaaagg agaaaatatt atggcaaaag ctttaattat
     2641 ttgtgcagca ggaatgtctt catcacttat ggcacagaaa acaacagagt ttctaaaagg
     2701 aaaagggaaa gatattgtag ttgatgctat tagtgctact gaaggaggga aagctatcgc
     2761 agcagcagct tttgatttgt atcttgtcag tcctcaaaca aaaatgtact ttaagcaatt
     2821 tgaagaggct ggttctaaag caggaaaacc agttgtacaa atacctcctc aagcctatat
     2881 tcctattcca atgggaattg aaaaaatggc taatttgatt cttgagaata tttgatgaac
     2941 acatgaaatg tcaataaata tttaagttaa gataaattgt tttgagtttg atataaattg
     3001 atttaagtgt ataatatgct tcaatatatg aaaacatacg ttttaggagg gaatgaatgt
     3061 gttaaacatt aaagagaaag atatccttca atttttaata aaaaataagg aacgttttgt
     3121 tacaagcaaa gaactggcag aatatttatc ctgttcagat agaactgtcc gtaatgtact
     3181 gaaattaatt gagaagacaa tgattattca aggagtccgg ttgatttcaa aacaaggtca
     3241 agggtatcaa atattttttg agaatcaagg cgcttaccag gagtttagac aaacttatga
     3301 actggaagaa gactatacga aaactgcggt ttcaaaaggt gatgatcgtt tagtatttat
     3361 cttaaataaa ttattatttg agcaagttcc agttcttttt gatgatttag ctgatgaact
     3421 ttacgttagt cgttcaacat tatctcatga tttcagaaag atacgagtga tgttatcaga
     3481 gtataatttg tccattgaga gtagggcgaa taaaggagtt tatgtctcag gcgaggaacg
     3541 tgataaacga cgttttatca taaattattt cctagagaat caatttttta aaacaataca
     3601 ttgctatgta aaatttaact tttttgctca aactgttcca ttagaagaat ttgctagaat
     3661 tgttttggat gaatgccaag aggccaacct aaaactatct gattttgtac tacagaattt
     3721 agtagttcac atagcattgt ctgtgattcg tttaaaatcc ggatttgaaa taaaaaatat
     3781 agactgtcag atgactgatg atgcaataga gagaaaggtt gctcaaagaa ttctttctaa
     3841 agtaagagaa gtaacaaatc aggaatttcc agttcaggaa atagactaca ttactttgca
     3901 tttgttagct aagagtcaac aatgtcaaaa aaatcagaag aatatttctg aagaagtttt
     3961 aaaaaaatct ttatttaaaa catttcagaa tttaggatta gatgatatgt ataacttttc
     4021 ttcagatttc caactaattg aaggattgat aactcatcta atgacactac aagtacgttt
     4081 agaaagccgg attaccttaa ataacccttt ggtagatgaa attaaacaga attatagtga
     4141 tatattcttt atgactagag aaattcttgc aaatatggac aagttcttgg agtggacaat
     4201 atcagatgat gaagtagcat atgtaagtct tcacttttta gctgctatgg aacgtagcaa
     4261 agagagtact aagtttaata ttcttgctat ttgtgcaaca ggatttggtg cagcccaaat
     4321 gttaagaaat cgtttggaaa cggagtttgg gaagcgagta gaagttgttg atgtaatagg
     4381 ctattatgaa ttgaatcaag aaaaacttaa aggaatagat tttatagtgt cagctgttga
     4441 tttatcaaat ctatattttc aaattccagt ttttaaagtt agtgtgtttc ttaagagtga
     4501 tgagatggaa atgattagaa aagctatgga tcagatgcaa gtttctagtt atgtacaatc
     4561 atcaaagatt aataaatttg aaaacaacag ctttagacag tattttagta aagaaaattt
     4621 tttaatatgt actgaatcag acaaggtaaa tttacttgaa aagatggttg agagtttatc
     4681 tgttggtgaa tcaaatgagt ttgaacaatc tttactttat ggaattaaac agcgcgaaga
     4741 attaagttca gttgtttttt ctgaaaaaat tgcagttccg catcctattc agccctttgg
     4801 tacagaggga aaggtttcgg ttgcaatctg taaagattcg ttactctggg ataatcaaag
     4861 ttcatatgtc cagctagtat ttcttctatc tccatcaata tatgggaacg aaggtttggc
     4921 aactgtgact aaaaagatag tctctctgac agagaatgat gagttgcaaa atcagctaat
     4981 atcttgcaat aactttgaag attttattag catatttgag aagataaaat aggaggttca
     5041 tatgaatcaa gaggaactgc aggttgcagc atttgaaatt atcctgcatt cgggaaatgc
     5101 aaggtcagaa atacatgaag catttgcaaa aatgagagaa ggtagttttg atgatgcaga
     5161 aagtaaatta aatcaatcaa atgagataat tcttgaagcg catcatgcac aaaccaaact
     5221 cttgcaagaa tacgcaagtg gagtggaaat taaaattgaa attatcatgg tacatgctca
     5281 agatcattta atgaccacta tgacattatt agaagtagca aaggaaatgt tagctcttta
     5341 taagaaagtt aactaaatat gtgttgttat ataaggagga aatgatatgg ataaaaaaga
     5401 acgacaaaag attgaacaac aacgtcgaga gatggctcta actaatactt tttttaatcg
     5461 atatcttctt ctacgttatt caatagcact cttctttttt gggaatattt attggctttt
     5521 gaatcagttt attaatccct cgcctattat tattttcccc attatgctca tagtattttc
     5581 tattttagca actgttgaac aatttaaact ctatggaaat aggaaagaaa agttgggaat
     5641 aacactaatg tttgttagaa tacaaatact tatttcaata ggattacttg ttttaacttg
     5701 gacaagttgg tttaagaatc tttttccgat ttttgaaaat aaccaagtag cacgtttatt
     5761 tgtttttgta gttcttctat taggtttagt tctgagtttg ttagacatta gaagaattaa
     5821 aaaaatttat aaacgaacag ataaggctta tcaacaattt gtccaattgg aaaagaactc
     5881 acttagttta taatcctgat ctagtataat ttgtatttga aactagtctt tagaaagtaa
     5941 aaattattat gttttattaa ggaggtctat ttatggatca acaaaacggg ttgtttggtt
     6001 ttcttgaaaa ccatgttatg ggaccaatgg gcaaacttgc tcagtttaaa gtagtgcgtg
     6061 ctatcacggc tgcaggtatg gctgctgtac catttactat tgtaggatca atgtttttgg
     6121 tattcagtat tttgccacaa gctttctcat tttggccaat tgtggcagat attttctctg
     6181 cttcatttga taaattcaca tcactttaca tggttgcaaa ctatgcgact atgggttctc
     6241 tatctcttta tttcgttcta tcacttgcat atgaattgac aaaaatttat gcagaggaag
     6301 aagaactcaa tatgaatcct cttaatggtg ccttgctcgc cttgatggct tttgtcatga
     6361 cagtaccgca aatcattttt gatggtggaa tgatgaagac tgtgacaagt ctaaaagaag
     6421 gtgcagtaat tgcagatgga tgggcaatgg gaaatggagt cgcacgtttt gggacaacag
     6481 ggatttttac cgcaatcatt atggcaattg tgactgttct tatttatcgt atgtgtgtta
     6541 aacataattg ggttattaaa atgcctgaag ctgttccaga aggagtttct cgtggattta
     6601 ccgctttggt tccgggattt gttgttgcat ttgttgttat ctttatcaac ggtcttcttg
     6661 tagcaatggg aacagatatt tttaaagtca ttgcaattcc atttggtttt gtatccaatc
     6721 tgactaattc gtggattggt ttaatgatta tttatctatt gactcaacta ctttggattg
     6781 taggtatcca cggtgcgaac attgtttttg catttgttag tccaattgct cttgctaaca
     6841 tggctgaaaa tgctgctggc gggcacttcg ctgttgcagg tgaattttct aatatgtttg
     6901 taattgcagg tggttctggt gcaactttag gactatgttt atatattgct tttgcctcta
     6961 aatctgaaca gcttaaagca ataggacgag catctgtagt tccagcctta tttaatatta
     7021 atgaaccatt aatttttgga ttacctatta tctataatcc agccttggct ataccattta
     7081 ttttagcacc aatggttact gctactattt attacgtagc gaattctcta aactttatta
     7141 agccaattat cgcacaggtt ccatggccaa ctccagtagg gattggagct ttcttaggga
     7201 cagcagatct tcgagctgta ttagttgctc tagtatgtgc atttgcagca ttcttagtct
     7261 atcttccatt catccgtgta tatgatcaaa aattggtgaa agaagagcaa ggtatctaat
     7321 aagtaaaaaa gtatactcga tgatagaatc catatagttt aaataatgag tgttaatatt
     7381 agcagtgaag aggtcttttt aagatattct tctctgcttt ttcatatttt ttcgctatgt
     7441 agggtatgaa gcaaaataga aagtagttcg aatagttgat aaacaggaaa ctgctgttat
     7501 ttaaaaaaaa caataaagta aagtatgtta tcgtagaata cgatacaata gtaaaaaagc
     7561 aaaaagaatg cgttttatat ttgggtttga ggtttataaa ataggataag cgtgctgaaa
     7621 acttagatat gacaaacaaa ataaaatttt taaaagctac atagaaacat ggtataatgt
     7681 atctaaagga ggtagcatat gtccttagat atagataaag aaaagatgac aatcatggga
     7741 atagcttttg aaaacagatc cgtttttaag agtgtttggt atgctttaag tacaaatatg
     7801 attgaaggat ggcgacctac ggttagtgat gttgaaaaat tacgagatga agctcttgct
     7861 ctggggatga cttaatgaag cgcaactgtt atgagtctta tgactatgtt gatccggata
     7921 atttatatac cgatccgggt tcttcagttc taagaaataa gcaggaagga aaaaagcacg
     7981 cgagcttgaa tatcgtatga ttgctagtaa gagtttgaaa ttatttatca atcctatctt
     8041 agttaatagt ttaaaagtag tttgtttcac aactgcataa gcttagaaaa gttgaaaagt
     8101 gataactctt taataaactc ttggtttgtt aaaagatatt ttgtccacta cattttttca
     8161 attgactatt atttgtcata gatataacag atagaacaga tagaacattg cattcagaat
     8221 aatgaaaaac gcatcaaaaa tggtgcgttc tgtttttatg ctaccataaa aggaggaaaa
     8281 caaattgaaa atttttaagg gagagtttta tcgaatctct gtattaacag acaagctagt
     8341 aaggttagaa tactctcaaa ctggaagttt tgaggataga acgacacaac ttatctataa
     8401 tagagatttt ggccaagttt cgttagatta tatcgagaca tcaaacgtac tagatattat
     8461 gacggactat tttcatctgc actttaataa aggagaattt aacgccgaaa atttatttat
     8521 agaattaaaa ggaaattttg ccgtatatgg tagtcgctgg tattttggtg aatctattga
     8581 aacgttaaaa ggaacagctc ggactctgga taaggcagat ggagcaatct cgttagaaga
     8641 tggaattatt agccgaaatg gtatagcctt attggatgat tctcaaggat ttatttggga
     8701 tgaacaatct ggttatattg agagagaaaa tcaaattgac ctgtatttct ttgcctatgg
     8761 gcatgattat agaggagcaa tcagagactt ttaccatttg actggttcaa cacccttgtt
     8821 gccaagatat gctttaggca attggtggag tagatattgg ccttatacgt cggatgaata
     8881 cttggattta atagacagat ttgaaacaga gaaaattcca ttatctatcg gtgtgttaga
     8941 tatggattgg catataactg acattccagc tcgttttgga agtggctgga caggatatag
     9001 ttggaataga aacttaatac caaatccaga acagttattg caacaacttc atgatagaaa
     9061 gttaaaactc tccttaaatg tccatcctgc tgatgggata cgggcttatg aagaagctta
     9121 tcctcaaatc gcaaaacggt tggggttaaa tgtagaacta gaagaacctg ctatttttga
     9181 tttttttaat ccctctttta gggaagccta ctttaaagat gttcattatg agctagaaaa
     9241 gcagggagta gatttttggt ggattgactg gcaacaaggg acacaaggta tgctagatcc
     9301 actttggctt ttaaaccatt atcactatca ggatagttgt aaaaatgcag aaggtggttt
     9361 gattttatca agatatgcag gtcctggtag tcaccgctac cctgttggtt tttcagggga
     9421 tactattatt agttggaatt ccttaaggtt tcaaccctat tttacagcga cagcatctaa
     9481 tatcggttat agttggtgga gtcatgatat cggtggacat atgttggggg attatgacga
     9541 agagctacaa actagatggc tacagtttgg tgtttttagt ccgattactc gattacatag
     9601 ttctagaagt ccttttaata gtaaagaacc ttggttcttt tcagaaacaa catctaagat
     9661 tatgaagaaa taccttcgtt tgagacatca gatgattccc tatctatata ccatgaatgt
     9721 aaagacacat gaggaaggtg ccccattaat cagtccaatc tattatttct atccagagaa
     9781 tgatgagagc tataatgttc caaaccaata cttttttgga acagaactga tggtggctcc
     9841 cattgtagaa aagatggatt tgacattcca atctgcaaaa gtagatgtat ggttccctga
     9901 aggtgaatgg tatgacttct tttcagagaa aaaatacaca ggtggtgtga agttaagtgt
     9961 ttatagggac atctcgacta cgcctgtgtt tgcaaaaagt ggtgcaatca ttcccttggt
    10021 tggttctgag ataggtatgg gtgttgattt acctgaggtt gtagattggt atgtattccc
    10081 agggaaacaa catttttttg aaatgcttga agatcaaaat ggtcaaagat ataaaacaag
    10141 attatcaatc gattgggaaa tgggaatggt agagttagca ttacaaggag attctagtat
    10201 cgttccaagc aatagaaaac atagaattca ttttaaagga acgaatgtgt ctataattga
    10261 attgccaaat aagaatgata cagctaaatt tgaatggaaa gataataaaa ggacatcttt
    10321 aaatgacgaa gtttttagac tactaaagac agcttctctt ccatatgaat taaaagatag
    10381 attgttaaat caattcatca atgcgaaaaa ctctcatgac ttaatgaata tcttgcatca
    10441 tcaggataag gaattgagag ggcgtttgtt ggaaatgata tttactagcc aaaactaatt
    10501 gaaataatta gaaagtcatt tgtttagtgt atatgaagat tctgtatggt attcgttatc
    10561 actggtcaca ttgaaaatgt gaaataagca cctacaacat agactttaca acacttcatg
    10621 tttcatatca tggggtgttt ttctatttaa gtcaaagccc atcaaaaaat ctcactattc
    10681 atcaggttaa aggaaaccta atcatagtga gattgtgaat tatagtagag tttgtatcgc
    10741 ctcttcaatt tgttttgggt ctgtttttga agaaaagcgt tcaaagactt gcccatctcg
    10801 accgatgaga aacttagcga aattccattc gattcgtttt cctagtgggc cggatttctg
    10861 gtcttttaac cagacataga gagggtctgc ttccttaccg ttgaccttaa tcttggcaaa
    10921 acgtgggaag gtggtttgaa aatgtaggct acagaaggcg ttgatttcct ctgcgctgcc
    10981 gggtgcttgt cccataaact gattgcaagg gaaatccaat atttcgaagc cctgatcttg
    11041 atagcgttca tagagttctt gaagtccctg gtactggggc gttaaaccac atccagtagc
    11101 agtgttgaca atcaagagaa ctttgccacg atagctatcc aagggagttg cttggttgtt
    11161 ttggttcaaa acggaaaaat cataaagtga agtcatgagg gcctcttttc tttttctaca
    11221 ttgtaacagt ttttactttt ttcgtcgagt cggatggaag atattgttga agaataagcc
    11281 ttcaatatga tagaaaccgt ttacaaataa ctttaaaaat ggtatgattt aattaaaccg
    11341 gtttacttag ttgctataaa tctctaattt gagatttagt tcctattgat tttacaatat
    11401 gtttattgga gtgtatacat gcaaacaaaa acaaagaagc tcattgtgag tttgtcttca
    11461 cttgttttat caggattttt attaaaccat tatatgacag ttggagcgga agaaacgact
    11521 acgaatacca ttcagcaaag ccagaaggaa gttcagtatc agcaaaggga tacaaaaaat
    11581 ttagttgaaa atggtgattt tggtcagacg gaggacggaa gcagtccgtg gacaggaagc
    11641 aaagctcagg ggtggtcagc ttgggtagac cagaagaata gtgcagatgc ctcaactcga
    11701 gtcattgagg ctaaggatgg ggctatcact atctcaagcc atgagaaatt aagggcagcg
    11761 gttcaccgta tggttcctat tgaagctaag aaaaagtata aactgcgttt caagattaaa
    11821 acagatcata aagtcgggat tgccaaagtt cgtatcattg aggaaagtga taaggacaag
    11881 cgattgtgga attctgcaac gacgtcagga acaaaggact ggcagaccat tgaagcagac
    11941 tatagcccga ctttagatgt tgataaaatc aagctggagt tattctatga aacaggaact
    12001 gggactgttt cctttaagga tattgagctg gtagaggtag cagaccagcc ttctgaggac
    12061 tatcaaacag ataaacagct tgaggaaaag attgatttac caattggaaa aaaacatgtt
    12121 ttttctcttg cggactatac ttataaggta gaaaatcctg acgttgcttc agtcaaaaat
    12181 ggaattttag aacctcttaa ggaagggaca accaatgtca ttgtcagtaa agatggcaag
    12241 gaagtgaaaa agattccttt gaagattcta gcctctgtta aggatgcata cacagaccgt
    12301 ttggatgact ggaatggcat catcgctggg aatcaatact atgattctaa aaatgaacag
    12361 atggccaaat taaaccagga attggaagga aaggtagctg atagcctatc tagtatttca
    12421 agtcaggcgg accgcaccta tttgtgggaa aaattttcaa attataaaat gtctgcaaat
    12481 ctgactgcca cttatcggaa attggaggag atggccaagc aagtgaccaa tccttcttct
    12541 cgttattatc aagatgaaac tgtcgttcga acagtcaggg attccatgga atggatgcat
    12601 aaacatgtct acaatagtga aaagagcatt gttgggaact ggtgggatta tgaaatcggt
    12661 acacctcgtg ccatcaacaa taccttgtct ctgatgaaag aatacttctc tgatgaggaa
    12721 attaaaaaat atacagatgt gattgaaaaa tttgtaccag atcccgaaca tttccgaaag
    12781 acgactgata acccatttaa ggctctaggt ggaaacttag ttgatatggg aagagtaaaa
    12841 gtaatagctg gtttactgcg taaggatgat caagaaattt cttctaccat tcgctcgatt
    12901 gagcaagtgt tcaagttggt agaccaaggt gaaggttttt atcaagatgg atcctatatc
    12961 gaccatacca atgttgccta tacgggtgct tatgggaatg ttttgattga tggcctgtct
    13021 caactgttgc cagtcattca aaagaccaag aatccaatcg ataaagataa aatgcaaacc
    13081 atgtaccact ggattgataa atcgtttgct cctttgctgg tgaatggaga gctgatggat
    13141 atgagtcgtg gacgctcgat cagtcgtgca aatagcgagg ggcacgtggc cgcagtagaa
    13201 gtactaagag ggattcaccg aatagcggat atgtctgaag gagaaaccaa acaacgtttg
    13261 cagagtcttg tgaagaccat tgttcaatcg gatagttatt atgatgtctt taagaatttg
    13321 aagacttata aggatatcag tttgatgcaa tccttgttaa gtgatgcagg agtcgcaagt
    13381 gttccaagaa caagttacct atctgccttt aacaagatgg ataaaacagc catgtacaat
    13441 gcagagaaag ggtttggatt tggcttgtca ctcttttcca gtcgtacctt gaattacgaa
    13501 cacatgaaca aggaaaataa acgtggttgg tatacgagtg atgggatgtt ctatctttac
    13561 aatggcgatt tgagtcacta tagcgatggc tactggccaa cagttaatcc atataagatg
    13621 cctggtacaa cagagacgga tgctaagaga gcggatagcg atacaggtaa agttttaccg
    13681 tctgctttcg ttggaacgag caaactagat gatgccaatg cgacagcaac catggatttc
    13741 accaactgga atcaaacatt gactgctcat aagagctggt ttatgctaaa ggataagatc
    13801 gcctttttag gaagcaatat ccaaaacact tcaacagata ctgctgcaac tacaattgac
    13861 cagagaaaac tggaatcaag taatccatat aaagtctatg tcaatgataa agaagcctcc
    13921 cttacagaac aagaaaagga ttatcctgaa acccaaagtg tctttttaga atcatccgat
    13981 tcgaaaaaga atattggtta ctttttcttt aagaagagtt caatcagtat gagtaaggct
    14041 ttgcaaaagg gagcctggaa ggatatcaat gaaggacagt cagacaagga agttgaaaat
    14101 gaatttctta cgattagtca ggctcataag caaaatggag attcttatgg ctatatgctc
    14161 atccctaacg tgggtcgtgc caccttcaat caaatgataa aagagttaga aagaagcctc
    14221 atcgaaaata acgaaaccct tcagtctgtt tatgatgcca aacaaggagt ttggggcatt
    14281 gtgaaatatg atgattctgt ctctactatt tccaaccaat tccaagtttt gaaacgtgga
    14341 gtctatacca ttcgaaaaga aggggatgaa tataagattg cctactataa tcctgaaacc
    14401 caggaatcag ctccagatca ggaagtcttt aaaaagctag agcaagcagc tcagccacaa
    14461 gtacagaatt caaaagaaaa ggaaaaatct gaagaggaaa agaaccattc ggatcaaaag
    14521 aatctccctc agacaggaga aggtcagtca atcttggcaa gtctagggtt cttgctactt
    14581 ggggcgtttt atttattccg tagaggaaag aacaactaat ttgttcatca tctagatgaa
    14641 ttacatgaaa ctgtggaaaa tagaaaaaat ccgaagaagg ccaaatatct tcggattttt
    14701 cttttaaaaa aggataagtt gatacttatc ctttttactt cattatgtga ctatagcgtt
    14761 ttgaaactag aataggacat cataactgct aaaagatttc tataaattca tttgattttc
    14821 ctaatcaatt tattcgtatc ctatttcact ctactatatt agtccgtctc gcacctcacg
    14881 gagcgagaca aaacaaccac ccgctatgcg ggtgcgcgtc gaaggttata ccaaaaaaac
    14941 tccaaacgcg atacaataaa ggtgttcaat ccacttgtaa agcgaaagga gaaaaaatat
    15001 ggcacaaaag gctcatagtt tatctcacac aaagtggagg tgttctatca cattgtgttc
    15061 acccctaagt atagacgaaa agtcatctat aatcaatata gaagtagttt aggcgaaata
    15121 tttcatcgct tgtgtagtta taaaggtgtt gaaatgatcg agggtcactt aatgccagac
    15181 catgtacaca tgttagtcag tattccacta aggctaagtg tttcgagttt catgggttat
    15241 ttaaaaggaa aaagtgcact catgatgttt gacaaacacg ccaatctcaa gtacaagttt
    15301 gggaatcggc atttctgggt ggaaggttat tatgtaagtc cagtagggct taatgaagcc
    15361 acaattaaga aatatagtca agattaaaga aattatccag tggatgattt cttcacgagt
    15421 atgaaaattt gagaactagt aaagtatgat atatagtaaa atgaaataag aacaggacaa
    15481 aatcgatcag gacagtcaaa tcgattttta acaatgtttt agaagtagag gtatactatt
    15541 ccagtttcaa tctactatag cactagataa attgagtgta aaagaatatg aggatccctt
    15601 tagggatagt ggtaagtaat accaaagtct cttaaagagg caagtgacga gtcaagagca
    15661 ataaggcttg aacaacgtaa aagccagcgt ctttaggcgc tggctgatga tttgggctta
    15721 tagctctggg ataaaccacc cgttagacgg gtagttatga ttttatctga gtgtaatata
    15781 ctgttgggca atctcgctga tgcggtcaaa gttgccttgg gaagcgagtt tattgagttc
    15841 gccaccaatt ccaacggcat ctgcaccagc agcgaaccat tgagggatgt tgtttaggcc
    15901 gactcctccg gttaccatta cggaaacttg tgggatcggt gccttgactg cagagatata
    15961 tgctggactg agagtactac ctgggaagag tttgatgatt tcactaccgg cttcaagtgc
    16021 agtcgtgatc tctgtgaggg taatacagcc tggaatgtac ggtgtgctgt agagattgca
    16081 cattttcgca gtttcagcat ggaaagatgg agaaacaacg taatttgctc cagctagaat
    16141 ggcatctcta gcagttacgg catcaagcac agtacctgca ccgatacaaa cactctgatc
    16201 gtcctgatac aagtctacaa gttccttgat gatttgtcct gcatactgat tggtataggc
    16261 gatttcaata gctttgatac cgcccttgat acaagcaatc gaggcttgta gtccttcttc
    16321 ctttgtattt cctcgaatga cagcgacaat tttctgtttt tttagttcaa taatcgtatc
    16381 tgatttggtc atgtaattct cctaacgaat gatatcttgt gcatttgcca gtaaattttc
    16441 aatactagtt gcggaagtgg agagatggtc tcctggaaga gtgcatttga gagttgcgct
    16501 cgcaactgca aagtcaatgg tagtttttag ggaggaatga tggagtagtt ggtaaagggc
    16561 accagatata aaggcatccc cgctaccaat tcgttgatag actgcagttt ttagttggac
    16621 agactcttca aatcgttctt ccagagcata ggcttgatag acatttttgt cttgctcatc
    16681 actagatcgg agggtatgga aaatggcctt gaaaccatag gcttctttta aaagtcgcat
    16741 gcgattttcc acctcttcta ggctagcact gtctcttgga aacatctcta gattttggtc
    16801 atcaatcatg agaggatcaa taccgaagca atagtcagta aaacgtgcaa acttagaaaa
    16861 ttcatacttg gcttctagga ctgaaatcat ctttgttctc agattgagat ccattgaaac
    16921 gacaattcct cggcgcttgg cttcttccaa gagtaggaga aggatcgcac ggacctcttg
    16981 accgatagct atggtgattc cactaaaatg aaaatggcta atcccctgaa agagagaatc
    17041 catatctagc atgtttggcc gaatctggct gatactcgta tgcttacgat cgtagaaaac
    17101 ttcactttga cgacaaccaa agccgttctc caaatagtag aggccgattc gatcgccaag
    17161 ccgacagatt gaactggtat cgatttggtg ctgtttcaag aatgtgagaa aacgatctcc
    17221 aatctcgttg gcagggagtg cggtaaagac tttcgttgag atacccaggg cttgcaaatt
    17281 acaagcgatg ttaatttctg atccgccaaa ataagtcgaa ctggcaacat gatcgccgat
    17341 actggtggca tctaatggtg agattcgaat taatggttcg ccaaacagta atattttcgt
    17401 catccattag ttcctttctg gtttagaata gctttacttg gctaatactt ctttcaaata
    17461 agcagcgact tcatcatctt ggcagttttc aaagaagtat tcttggaagt atttgccaga
    17521 gatggtgtct ttgaggaagt cttggtcgat tgttttaagg atggtcatca aatcattgtg
    17581 ggtgatttgt ttcacctcgt ttaagatacg agcgttgcgt tgttcaggga ttacacgttc
    17641 tcttgggtag ccaccgccca tttcttcagc aaataagcgt tcaaacatca atttgagggt
    17701 cagttctgcc ccccagccaa atcctttggc ataagggata gacaaggcgt taccaccatt
    17761 gatttgagaa taaaggtaag cgtcagttgg gtccactgct agaccacaga caacaccagg
    17821 gaagctgttt aaagcaagca tagcccctac acccgtacca cagccggtaa caacaaagtc
    17881 aactgccttt gtatttaaaa ggatggcagc cattagtccg ttctgcacat aagttaattg
    17941 actttctcct tcttctccac gcataccata gttaaataat tggtagtctt ttttatctgt
    18001 cgcttctttt agactatcgt aaataatgtg attcttgcta gcttgactat tttcattgat
    18061 taatgcgatt ttcataagat cttctttcta tttttactca ggttgttttc cgatgtaggc
    18121 taagataccg ccatctacat ataggatgtg gccattgaca aaattgctgg catcactagc
    18181 gagaaagaca gcagggccca tcaaatcttc aggatttccc caacgtgcag caggtgtttt
    18241 tgcaatgatg aactggtcaa atgggtggcg agaaccatct tcttgcaact cacgaagagg
    18301 tgctgtttga ggagtggcaa tataacccgg tccaattccg ttacattgga tattggctcc
    18361 accgtattca gacgcaatgt tgcgggtcaa cattttcaag ccccctttag cagcagcata
    18421 agcgctaact gtttcacgtc ccagttcgct catcatcgaa caaatattga taatctttcc
    18481 atgccctttc tttatcatag aaggaataac tgcctttgaa acgataaatg gtgcgtttaa
    18541 gtcaatatcg atgaccttac ggaaatcagc ggcgctcatt tcgcacattg gaactcggcg
    18601 gataatacca gcgttattaa cgaggatgtc aatgacacca acctcttgtt cgatttgctt
    18661 gaccatggct tggataccgt cctcgtctgt cacgtcacag acatatccat gggcttcgat
    18721 gccaacttca cgataagctt caatcccttt attgaccaga tcttgattga tatcgttaaa
    18781 gacaatagta gcgccggctt cagcgtagga tttggcaata gcaaatccaa ttccataaga
    18841 agcgccggtg atgagagcaa tttttccttt taaagaaaac tgctcaattg agaatgatgt
    18901 atttgtcatt tgatacttcc tttcaaattt gttaagactt ctctataacc aaattatatt
    18961 ctagcaaacc ggttttgtca ataaataaat atttttattt ataaaaagat ttgttttcct
    19021 gaaaaacgga atgttttgaa atttctgttt attaaaatgg ttaaaaagat aatgaaagcg
    19081 ctaatatttt tttaaaaaaa ctattgacaa gataaaaata aaggattaca atggggaata
    19141 taaagtaaac cggtaaacct aaacaagaaa ggagaaaaga tgaaaattgt acttgtaggg
    19201 catggacatt ttgctacagg gatttatagt tctttacaat tgattgcagg taatcaagaa
    19261 aatgtggagg cgattgactt tgtggaagga atgtcagcag atgaactcaa gcaaaaaatc
    19321 ttacttgcaa tttcaaatga agaagaagtt ttaatcctaa gtgatctctt gggaggatcg
    19381 ccattcaagg tttcttctac cataatggga gaaaatccag tcaaaacaat gaatgttctc
    19441 tcgggtttga acttagccat gttaatggaa gcagtctttg ctagaatggc tcatagcttt
    19501 gatgaggttg ttaataaatc agtagtggcg gcccagggcg gagtcgtaaa tggtaaagaa
    19561 ttgttttcaa cggatgcaga ggaagaagat ttcgaatcgg gtatttaaag ggtaaaagaa
    19621 tgataaaaaa ggttacgatt gaaaaaataa aatcgcctga gcgcttctta gaagtaccac
    19681 ttctgacgaa agaagaagtc ggccaggcaa tcgataaggt tattcggcag ttagaactca
    19741 accttgacta tttcaaggaa gatttcccga cgccagctac ctttgataat gtctatccaa
    19801 tcatggataa cacggaatgg accaatggtt tctggacagg agaactgtgg ttggcttatg
    19861 aatacagtca acaggatgca tttaaaaaca tcgctcataa aaatgttctt tctttcctgg
    19921 atcgtgtcaa taagagagta gaattggatc accatgatct cggcttcttg tacacaccgt
    19981 cttgtatggc tgaatataag ataaatggag atggagaggc tagagaagca accttgaaag
    20041 ctgcagataa gttgattgaa cgctatcaag aaaaaggtgg ttttattcaa gcttggggag
    20101 acttgggcaa gaaagagcat taccgtttga ttatcgactg cttgctcaat atccaactct
    20161 tattctttgc ttatcaagaa acaggcgatc aaaaatacta cgatattgca gaaagtcatt
    20221 tctatgcttc agctaataat gtaatccgtg atgacgcttc gtccttccac accttctatt
    20281 ttgatcctga gacaggtcaa ccctttaaag gtgtaacgag acaagggtat agtgatgatt
    20341 catgctgggc acgtggtcaa tcatggggag tctatggtat tcctttgact tatcgtcact
    20401 taaaagacga gtcctgcttt gacttgttta agggtgtgac caattatttc ttgaatcgtc
    20461 tgccaaaaga tcatgtgtcc tattgggatt tgatttttaa tgatggtagt gctcaatcac
    20521 gagattcttc agcaacagct atcgccgtct gtgggattca tgaaatgcta aaacatctcc
    20581 cagaggtgga tgctgacaaa gatatttata aacatgctat gcatgccatg cttcgttcct
    20641 tgatcgaaca ttatgcaaat gatcaattta cccctggtgg gacaagtctt ctccacggtg
    20701 tgtactcatg gcattcaggt aaaggagtgg atgaaggcaa tatctggggt gactactatt
    20761 acctagaagc ccttatccgt ttctacaaag actggaacct atattggtag gaggagaaat
    20821 atgacaatgc caaatattat tatgacccgt atcgatgaac ggttgattca tggacaagga
    20881 caactttggg taaaatacct agcttgtaat acggtcattg ttgccaatga cgaagtaagc
    20941 acggacaaga tgcaacaaac tctgatgaaa acagttgtgc cagactcagt tgccatgcgt
    21001 ttcttccctt tgcaaaaggt gattgatatc attcacaagg ctaatcctgc tcaaacgatc
    21061 tttatcgttg taaaggatgt aaaggacgct ttaaccttgg tagaaggtgg tgtccctatc
    21121 aaagaaatca atattgggaa cattcacaat gcccctggta aagagcaagt gacacgctcc
    21181 atcttcctgg gtgaagagga caaggcagcc ctcaaggaat taagccaaac tcatcaagta
    21241 acatttaata cgaaaacaac tccaacagga aatgatggag ctgttcaagt caacattatg
    21301 gactatattt aaaagaggag atcgttatgt cgattaatgt atttcaagcg attttaattg
    21361 gattatggac agctttctgt tttagtggaa tgctgttagg aatttacacc aatagatgta
    21421 ttgttctgtc atttggtgtc ggaattattc taggtgatct gcctactgct cttgcaatgg
    21481 gagctattgg tgaattggct tatatgggat tcggtgttgg tgctggaggt actgttccac
    21541 caaacccaat cggacctggt atctttggta ccttgatggc tatcactagt gctggtaaag
    21601 tcagtccaga agcggctctt gccctctcta ctccgattgc tgtggcgatt caattcttac
    21661 aaactttcgc ctacactgta cgtgctggtg cgcctgaaac ggcgatgaag cacttgaaaa
    21721 accataattt gaagaaattc aagtttactc taaatgcaac aatttggttg tttgccttta
    21781 ttggatttac cttgggttgc ttgggtgccc tttcaatgga taccttgttg aaactcgtag
    21841 actacattcc accggtatta cttacaggtt tgacagttgc tggtaaaatg ctcccagcta
    21901 tcggttttgc gatgatcttg tcagtgatgg ctaagaaaga gttgattccc tttgtcttgt
    21961 tgggatatgt ttgtgcagct tatctaaaca tcccaacaat tggtattgca attgtaggta
    22021 ctatctttgc tttgattgaa ttttataaca agccaaaaac agcgaatcat gtggtagagg
    22081 aggaagcaca cgatgactgg atctaacaaa ttaacaaaac gtgattatct taaaacgtct
    22141 ttgcgggcat tcttttgtca aaatggattt aactatagta actatcaagg gttgggatat
    22201 gccaatgtga tgtatcctgc tttgaaaaaa cactatggag aggatcagga aggtttctac
    22261 caagccttgg aagaaaactg tgaattctat aataccaacc cacacttcct gccttttatt
    22321 accagcttgc atcttgtaat gttggaaaat ggccgtccgg caaaagaaac acgtagcatc
    22381 aagatggcct tgatgggacc attggcaggt attggggatt ctctttctca attctgttta
    22441 gctccattgt tctcaaccat cgcagcttcg tttgctcaag aaggcttggt tgtcggtcca
    22501 atcttgttct tccttgcgat gaatacgatt ttaacagcga ttaaattgtc aactggtctg
    22561 tatggataca aactaggaac aactgtgatt gataaactaa gcgaacagat ggcaacgatt
    22621 tctcgtattg ccaatattat cggtgtaacc gtaattgctg gtttggcagc gacatctgtt
    22681 aagattatgg tgccgattac ctttgctgca ggggaagtta aagcagacgc taaacaaagt
    22741 atcgtaagta ttcagggaat gcttgataag gttgctccag ctcttctacc agccctattt
    22801 acacttttag tttactactt gatcaaagaa aagaaatgga caacatataa actcgttatt
    22861 ttaacagttg tcatcggaat tatcggaagc tggcttaaga ttatagctta atggatacaa
    22921 cattgtttta tggaatagtg attgtcttgg cagtgagtcc ccttttactg tcaagctttc
    22981 attctattcg tcagcaaaag ttgcttcgca aacagacgga gcaacgacaa gagtatttag
    23041 cttctttaac atctggtgat gaagtgttgt tattgtctgg aattcatgga aaaatcattt
    23101 ctatcaaaga tgacttggtc tccttgcaga ttgcaaaagg tgtggtcatc tatgtagaaa
    23161 aggaaagtgt aatgggaaag acaaaagaac tgctttttaa gtagttcttt ttctggtaat
    23221 agataatgaa agaagaaaga agacaatttt ttgaaagagt cgatggaaac caatgtcgtg
    23281 attatatctt gtcgcactgt tcaaaagact atgagaaagt caagagttcc cttgaacgct
    23341 tgatggacaa tcgttttatg tttgatagtc cttgggatat ggagccttgt tcaaaaatcc
    23401 atcaaatcca gccgatggta tgggatcaag tatttgaaga tgatccagaa tgggcttata
    23461 tgctcaatcg acaagaatat ctcttgcagt ttatgatagg gtatgtggta gaaggagata
    23521 aggactatat tcaaaagtgc aagttctttc tatttgattg gattgagcag gtgagagaat
    23581 tttctcctca atccttgatg actagaacct tggatacggg tattcgttcc tttacttggt
    23641 tgaaactact cttgctcctc ttgaaatttg acatgctaga ggagaaagaa ctagagaaaa
    23701 ttttggtcag tctagaaaag cagattgact ttatgaaaag ctactatcgc gccaagtaca
    23761 cccttagtaa ctgggggatt ttacaaacaa ttccgatgct tgctatctat tatttctttt
    23821 cagataagat ggacctagaa gaagcttacc attttgcttc agaggagttg aaacagcaaa
    23881 ttgagacaca gattttagga gatggaagcc agtttgaaca gtcgattctc tatcatgtag
    23941 aggtttataa agccttgctg gatttgtgtc tcttgcttcc agacttgcaa gatagttacc
    24001 gagagttgct ggaaaagatg gcgacctata ttcaaatgat gacaggctta gatggacgaa
    24061 ctttggcttt tggtgatagc gattctacag aaacgacaga aattttgagc ctgtctgctg
    24121 tggttttgaa caaggaagac cttcttaacg gtctggatgt taaagtcgat ttgcttagcc
    24181 tcttgttcct ggggcgagaa aaggtcaagc gactgcagga atttgaaaag agagcttggc
    24241 agcctaagtc catgatcttt gaagactctg gacatgtctg cattaaggat gaacatcgtt
    24301 atctattttt caaaaatggt ccgctaggaa gtgcccatag ccatagtgac gagaatagtt
    24361 tttgcttaca gtatcaagga caacctattt tcatagatgc tgggcgttat tcttatcggg
    24421 agatatatga acgttatctc ttaaagagtg cttggagtca ttcgacctgc attgtagatg
    24481 ggaaagctcc ggaaagaatc acgggatcct gggaatatga atactatcct cactccctgt
    24541 tttgtcacca taaagaaagg gagggagtgc attatattga aggggcttat tggtcagcag
    24601 aacctgattt gccttatctt cacaagagaa aaatcctcat gttggtagag gatgtctggc
    24661 tcttggtgga tgacatcagg tgtcaaggtc agcatgaggc gttgactcag tttatccttg
    24721 acaaggatgt gacctatcaa gatgggaaaa tcaatcagtt gagactatgg agtgaagttg
    24781 attttgattt ggaagatacc atcatttctc ctaaatacaa tgagcttgaa agaagtaaca
    24841 aactcaccaa gcgccaattc tttgagaatc agatgctgga ttataccatc attgcgcatg
    24901 agagttttga aatcatccgt cattctgtct accagacaga tgatcgtgaa gtggaaaatg
    24961 ctctggcttt tgaagtgaaa aatgacgaaa cagacaagct gattctgtta ttaagcgagg
    25021 atatttgtgt aggtgaaaaa ttgtgcctcg ttgacggaac aaaaatgcgt ggaaaatgtc
    25081 tagtatatga taaaataaat gagagaatga ttcgcttgca gtgctagaaa taggcatttt
    25141 gaatagtgaa tatgttataa taagtattag taggaggtgt tttagattgg agaagaaact
    25201 gaccataaaa gacattgcgg aaatggctca gacctcgaaa acaaccgtgt cattttacct
    25261 aaacgggaaa tatgaaaaaa tgtcccaaga gacacgtgaa aagattgaaa aagttattca
    25321 tgaaacaaat tacaaaccga gcattgttgc gcgtagctta aactccaaac gaacaaaatt
    25381 aatcggtgtt ttgatcggtg atattaccaa cagtttctca aaccaaattg ttaagggaat
    25441 tgaggatatc gccagccaga atggctacca ggtaatgata ggaaatagta attacagcca
    25501 agagagtgag gaccggtata ttgaaagcat gcttctcttg ggagtagacg gctttattat
    25561 tcagccgacc tctaatttcc gaaaatattc tcgtatcatc gatgagaaaa agaagaaaat
    25621 ggtctttttt gatagtcagc tctatgaaca ccggactagc tgggttaaaa ccaataacta
    25681 tgatgccgtt tatgacatga cccagtcctg tatcgaaaaa ggttatgagc attttctctt
    25741 gattacagcg gatacgagtc gtttgagtac tcggattgag cgggcaagtg gttttgtgga
    25801 tgctttaaca gatgctaata tgcgtcacgc cagtctaacc attgaagata agcatacgaa
    25861 tttggaacaa attaaggaat ttttacaaaa agaaatcgat cccgatgaaa aaactctggt
    25921 atttatccct aactgttggg ccctacctct agtctttacc gttatcaaag agttgaatta
    25981 taacttgcca caagttgggt tgattggttt tgacaatacg gagtggactt gcttttcttc
    26041 tccaagtgtt tcgacgctgg ttcagccctc ctttgaggaa ggacaacagg ctacaaagat
    26101 tttgattgat cagattgaag gccgtaatca agaagaaagg caacaagtct tggattgtag
    26161 tgtgaattgg aaagagtcga ctttctaaaa tgaaggaaaa tgacttgcaa tctctgttaa
    26221 gaaataaaat aatcccacct agaacaagct aggtgggatt atttgcttat gaaatgagaa
    26281 attatgggag caagctccta aatcaactgt ttttgatcta cttctttaac tacttgataa
    26341 aagttataga agtaggccaa acttgaaatg atggttacga ctaggaatat tgaaaatttc
    26401 cattggacag ggttggttaa aagttgtgga aaggatatga ggagaaagaa gagggctgcg
    26461 ttgaggacag gtatccgttt tgattgtatt ttctcaagtc ctttattgag cgcaggaaga
    26521 aagaggagta ggagtagtaa aactgtatga gaaacagctc ctgaaataag ggcgaagaaa
    26581 aggaaaatac tgataaaaac atgaatgttc agtagtctag ctagtgattt cataaggcac
    26641 ctcctaatcc tggtcttttt tagctcttgc aatacgaagt gagtcgacaa tatgtatcat
    26701 cactccgaaa aagaaagctc ccagtatagt tttaaaaata tgttttgtat ttagaagaga
    26761 actgataaaa tttggatttt cacttgttag ggtatcaatg agtggaatta taaaaaatat
    26821 cactgttcca taaatcgaac ctgctttcag accaggataa cgtaactgtt tcttttcttt
    26881 tttcatgagt ttcctcctaa tcctcatctt gatttttctt agtttttgca atgcgacggg
    26941 agatgaggaa ctgtatgctc gctccgaaga aaatagaacc gagaatactt gatacaccat
    27001 ttcttatagt gagaagagaa tgaaaatagt cctgaccttc atctatgagt atactgagaa
    27061 gaggagttat aaaaaacatc catagaccaa agaacaaacc tgctttcaga cctgggtagt
    27121 gtagttgctt gctttctttc tcattcagca tatctggttc aatgactgtg atgcctgttt
    27181 ttttcatttg gtaggtgaca tagccagcag cgatgagggc aatcactaaa atcagaggag
    27241 gatagattag agccacttct tgagggtatt tataggctag aaggagtgga ataagatttc
    27301 cgaaaatcat cagataaaag aggacgataa agacttggtt cccaatacta tcggcctcac
    27361 gccgtttgta ttcgtcaagg ggaccagaaa taccgtatgt gcgtttgatc agtttttcag
    27421 tgaaggtttc ttttttcatg agtttgctcc ttttttaaaa atcttcctcc caaaagagac
    27481 tgttgaggtc agtttggagg ctgcgggcga gattgagaca gagttccaag gttggattgt
    27541 acttgtcgtt ttcaatcata ttgatagtct gtctcgagac accgatatcc ttggcgagtt
    27601 cgagctggga aatacccaat tccttgcgaa attctttcac acgattcatc tggtctcctt
    27661 tctgatttat gtcgtatata tttgactata ttatagtctt ttaaacataa agtgtcaagt
    27721 atatttgaca tattttttga agaaatagta gtctccttgt cctatttgtc tgacaagtgc
    27781 aagctggtcg gatttgtggt aaaatagata agatatgaca aaagaatttc atcatgtaac
    27841 ggtcttactc cacgaaacga ttgatatgct tgacgtaaaa cctgacggta tctacgttga
    27901 tgcgactttg ggtggagcag gccatagcga gtatttatta agtaaattaa gtgaaaaagg
    27961 ccatctctat gcctttgacc aggatcagaa tgccattgac aatgcgcaaa aacgcttggc
    28021 accttacatt gagaagggaa tggtgacctt tatcaaggat aacttccgtc atttacaggc
    28081 acgtttgcgc gaagctggtg ttcaggaaat tgatggaatt tgttatgact tgggagtgtc
    28141 tagtcctcaa ttggaccagc gtgagcgtgg tttttcttat aaaaaggatg cgccactgga
    28201 catgcggatg aatcaggatg ctagtctgac agcctatgaa gtggttaatc actatgacta
    28261 tcatgatttg gttcgtattt tcttcaaata cggtgaggat aaattctcta aacagattgc
    28321 gcgtaagatt gagcaagcgc gtgaagtgaa gccgattgag acaacgactg agttagcaga
    28381 gattatcaag ttggtcaaac ctgccaagga actcaagaag aagggtcatc ctgctaagca
    28441 gattttccag gctattcgaa ttgaagtcaa tgatgaactg ggagcggcag atgagtccat
    28501 ccagcaggct atggatatgt tggctctgga tggtagaatt tcagtgatta cctttcattc
    28561 cttagaagac cgcttgacca agcaattgtt caaggaagct tcaacagttg aagttccaaa
    28621 aggcttgcct ttcatcccag atgatctcaa gcccaagatg gaattggtgt cccgtaagcc
    28681 aatcttgcca agtgcggaag agttagaagc caataaccgc tcgcactcag ccaagttgcg
    28741 cgtggccaga aaaattcaca agtaagaggg aaaaagatgg cagaaaaaat ggaaaaaaca
    28801 ggtcaaatac tacagatgca acttaaacgg ttttcgcgtg tggaaaaagc tttttacttt
    28861 tccattgctg taaccactct tattgtagcc attagtatta tttttatgca gaccaagctc
    28921 ttgcaagtgc agaatgattt gacaaaaatc aatgcgcaga tagaggaaaa gaagaccgaa
    28981 ttggacgatg ccaagcaaga ggtcaatgaa ctattacgtg cagaacgttt gaaagaaatt
    29041 gccaattcac acgatttgca attaaacaat gaaaatatta gaatagcgga gtaagatatg
    29101 aagtggacaa aaagagtaat ccgttatgcg accaaaaatc ggaaatcgcc ggctgaaaac
    29161 agacgcagag ttggaaaaag tctgagttta ttatctgtct ttgtttttgc cattttttta
    29221 gtcaattttg cggtcattat tgggacaggc actcgctttg gaacagattt agcgaaggaa
    29281 gctaagaagg ttcatcaaac cacccgtaca gttcctgcca aacgtgggac tatttatgac
    29341 cgaaatggag tcccgattgc tgaggatgca acctcctata atgtctatgc ggtcattgat
    29401 gagaactata agtcagcaac gggtaagatt ctttacgtag aaaaaacaca atttaacaag
    29461 gttgcagagg tctttcataa gtatctggac atggaagaat cctatgtaag agagcaactc
    29521 tcgcaaccta atctcaagca agtttccttt ggagcaaagg gaaatgggat tacctatgcc
    29581 aatatgatgt ctatcaaaaa agaattggaa gctgcagagg tcaaggggat tgattttaca
    29641 accagtccca atcgtagtta cccaaacgga caatttgctt ctagttttat cggcctagct
    29701 cagctccatg aaaatgaaga tggaagcaag agcttgctgg gaacctctgg aatggagagt
    29761 tccttgaaca gtattcttgc agggacagac ggcattatta cctatgaaaa ggatcgtctg
    29821 ggtaatattg tacccggaac agaacaagtt tcccaacgaa cgatggacgg taaggatgtt
    29881 tatacaacca tttccagccc cctccagtcc tttatggaaa cccagatgga ttcttttcaa
    29941 gagaaggtaa aaggaaagta catgacagcg actttggtca gtgctaaaac aggggaaatt
    30001 ctggcaacaa cgcaacgacc gacctttgat gcagatacaa aagaaggcat tacagaggac
    30061 tttgtttggc gtgatatcct ttaccaaagt aactatgagc caggttccac tatgaaagtg
    30121 atgatgttgg ctgctgctat tgataataat acctttccag gaggagaagt ctttaatagt
    30181 agtgagttaa aaattgcaga tgccacgatt cgagattggg acgttaatga aggattgact
    30241 ggtggcagaa tgatgacttt ttctcaaggt tttgcacact caagtaacgt tgggatgacc
    30301 ctccttgagc aaaagatggg agatgctacc tggcttgatt atcttaatcg ttttaagttt
    30361 ggagttccga cccgtttcgg tttgacggat gagtatgctg gtcagcttcc tgcggataat
    30421 attgtcaaca ttgcgcaaag ctcatttgga caagggattt cagtgaccca gacgcaaatg
    30481 attcgtgcct ttacagctat tgctaatgac ggtgtcatgc tggagcctaa atttattagt
    30541 gccgtttatg atccaaatga tcaaactgct cggaaatctc aaaaagaaat tgtgggaaat
    30601 cctgtttcta aagatgcagc tagtctaact cggactaaca tggttttggt agggacggat
    30661 ccggtttatg gaaccatgta taaccacagc acaggcaagc caactgtaac tgttcctggg
    30721 caaaatgtag ccctcaagtc tggtacggct cagattgctg acgagaaaaa tggtggttat
    30781 ctagtcgggt taaccgacta tattttctcg gctgtatcga tgagtccggc tgaaaatcct
    30841 gattttatct tgtatgtgac ggtccaacaa cctgaacatt attcaggtat tcagttggga
    30901 gaatttgcca atcctatctt ggagcgggct tcagctatga aagactctct caatcttcaa
    30961 acaacagcta aagctttgga gcaagtaagt caacaaagtc cttatcctat gcctagtgtc
    31021 aaggatattt cacctggtga tttagcagaa gaattgcgtc gcaatcttgt acaacccatc
    31081 gttgtgggaa caggaacgaa gattaaaaac agttctgctg aagaagggaa gaatcttgcc
    31141 ccgaatcagc aagtccttat cttatctgat aaagtagagg aagttccaga tatgtatggt
    31201 tggacaaagg agactgctga gacccttgct aagtggctca atatagaact tgaatttcaa
    31261 ggttcgggct ctactgtgca gaagcaagat gttcgtgcta acacagctat caaggacatt
    31321 aaaaaaatta cattaacttt aggagactaa tatgtttatt tccatcagtg ctggaattgt
    31381 gacattttta ctaactttag taggaattcc ggcctttatc caattttata gaaaggcgca
    31441 aattacaggc cagcagatgc atgaggatgt caaacagcat caggcaaaag ctgggactcc
    31501 tacaatggga ggtttggttt tcttgattac ttctgttttg gttgctttct ttttcgccct
    31561 atttagtagc caattcagca ataatatggg aatgattttg ttcatcttgg tcttgtatgg
    31621 cttggtcgga tttttagatg actttctcaa ggtctttcgt aaaatcaatg aggggcttaa
    31681 tcctaagcaa aaattagctc ttcagcttct aggtggagtt atcttctatc ttttctatga
    31741 gcgcggtggc gatatgcttt ctgtctttgg ttatcaagtg catctaggga ttttctatat
    31801 tgtcttcgct cttttctggc tagtcggttt ttcaaacgca gtaaacttga cagacggtgt
    31861 tgacggttta gctagtattt ccgttgtgat tagtttgtct gcctatggag ttattgccta
    31921 tgtgcaaggt cagatggata ttcttctagt gattcttgcc atgattggtg gtttgctcag
    31981 ttttttcatc tttaaccata agcctgctaa ggtctttatg ggtgatgtgg gaagtttggc
    32041 cctaggtggg atgctggcag ctatctctat ggctctccac caagaatgga ctctcttgat
    32101 tatcggaatt gtatatgttt ttgaaacaac ttctgttatg atgcaagtca gttatttcaa
    32161 actgacaggt ggtaaacgta ttttccgtat gacgcctgta catcaccatt ttgagcttgg
    32221 gggattgtct ggtaaaggaa atccttggag cgagtggaag gttgacttct tcttttgggg
    32281 agttgggctt ctagcaagtc tcctgaccct tgcaattttg tatttgatgt aagaatggca
    32341 ccctgatgtt tcagggtgtt tttgtgttta aatacacaat gaaaatcaaa gaacaaacta
    32401 gaaagctaac tttaggctgc tcaaaatata gtagattgaa actagaatag tacacctcta
    32461 cttctaaaac atttttagaa atcgatttga ctgtcctgaa cgatttatcc tgttcttatt
    32521 tcattttact atacagtttt gagatggttg atagaactga cgaagtcagc tcaaaacact
    32581 gttttgaggt tgtagacaag gcgaagctga tgtggtttga agaggttttc gaagagtgta
    32641 aaaaaatatt ggtcagttaa agtcaaaact cttgaccttt tctgaccaat gtagtatatt
    32701 atgaacgtaa ggtaaatgaa agccttacta gaacacttat ttaaagtaaa atagaaagag
    32761 gtgagtctat gtttcatcta gcaatcttca gaggtaaaga tagtctactc ctgcttgaaa
    32821 aagaaaaacc agaaatagta cgtagggtag cgatctagct agtctaaatt ttttaaaaca
    32881 atggtcaaag atagtcaata tcagtaatca taactaagta aacaaaaaga ggtaaagaat
    32941 atgaataaca actttaataa ttttaataac atggatgatt tatttaacca attgatgggt
    33001 ggtatgcgag gatatagttc tgaaaatcgc cgttacttaa ttaatggacg cgaagtcaca
    33061 cctgaggaat ttgctcacta tcgtgcgact ggtcaattac caggaaatgc agaaactgat
    33121 gtgcaaatgc cacaacaggc atcaggtatg aaacaagacg gtgtccttgc aaaactaggt
    33181 cgaaacttaa cagcagaagc gcgtgagggc aagttggatc ctgttatcgg acgaaacaag
    33241 gaaattcaag aaacatctga aatcctctca cgccgcacca agaacaatcc tgttttggtc
    33301 ggagatgcag gtgttggtaa gacagcagtt gtcgaaggtc tagcgcaagc cattgtgaac
    33361 ggagatgttc ctgctgctat caagaacaag gaaattattt ccattgatat ctcaggtctt
    33421 gaggctggta ctcaataccg tggtagcttt gaagaaaatg tccaaaactt agtcaatgaa
    33481 gtgaaagaag cagggaatat tatcctcttc tttgatgaaa ttcaccaaat tcttggtgct
    33541 ggtagcactg gtggagacag tggttctaaa ggacttgcgg atattctcaa gccagctctc
    33601 tctcgtggag aattgacagt gattggggca acaactcaag acgaataccg taacaccatc
    33661 ttgaagaatg ctgctcttgc tcgtcgtttc aacgaagtga aggtcaatgc tccttcagca
    33721 gagaatactt ttaaaattct tcaaggcatt cgtgacctct atcaacaaca ccacaatgtc
    33781 atcttgccag acgaagtctt gaaagcagcg gtggattatt ctgttcaata cattcctcaa
    33841 cgtagcttgc cagataaggc tattgacctt gtcgatgtaa cggctgctca cttggcggct
    33901 caacatccag taacagatgt gcatgctgtt gaacgagaaa tcgaaacgga aaaagacaag
    33961 caagaaaaag cagttgaagc agaagatttt gaagcagctc taaactataa aacacgcatt
    34021 gcagaattgg aaaggaaaat cgaaaaccac acagaagata tgaaagtgac tgcaagtgtc
    34081 aacgatgtgg ctgaatctgt ggaacgaatg acaggtatcc cagtatcgca aatgggagct
    34141 tcagatatcg aacgtttgaa agatatggct catcgcttgc aagaaaaggt gattggtcaa
    34201 gataaggccg tagaagttgt agctcgtgct atccgtcgta accgtgctgg ttttgatgaa
    34261 ggaaatcgcc caatcggcag cttcctcttt gtagggtcta ctggggttgg taagacggag
    34321 cttgctaagc aattggcact cgatatgttt ggaacccagg atgcgattat ccgtttggat
    34381 atgtctgaat acagtgaccg cacagctgtt tctaagctaa ttggtacaac agcaggctat
    34441 gtgggttatg atgacaatag caatacctta acagaacgtg ttcgtcgcaa tccatactct
    34501 atcattctct tggatgaaat tgaaaaggct gatcctcaag ttattaccct tctcctccaa
    34561 gttctagatg atggtcgttt gacagatggt caaggaaata cagtaaactt caagaatact
    34621 gtcgttattg cgacctcaaa tgctggattt ggctatgaag ccaacttgac agaagatgcg
    34681 gataaaccag aattgatgga ccgtttgaaa cccttcttcc gtccagaatt cctcaaccgc
    34741 tttaatgcag tcatcgagtt ctcacacttg actaaggaag acctttctaa gattgtagat
    34801 ttgatgttgg ctgaagttaa ccaaaccttg gctaagaaag acattgactt ggtagttagt
    34861 caagcggcta aagattatat cacagaagaa ggttacgacg aagtcatggg ggttcgtcct
    34921 ctccgtcgcg tggttgaaca agaaattcgt gataaggtga cagacttcca cttggatcat
    34981 ttagatgcta aacatctgga agcagatatg gaagatggcg ttttggttat tcgtgagaaa
    35041 gcctaagata gaattttgag gataaaaaag aaggagccag ctgaaaaaaa ctggttcctt
    35101 tttaggtacg acaggcatgt cgtatagtag aagtgtatta ttccagtttc aat
//
LOCUS       DAVBCV010000016        34466 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBCV010000016 DAVBCV010000000
VERSION     DAVBCV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.016"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            143..361
                     /gene="infA"
                     /locus_tag="VQ516_000775"
     CDS             143..361
                     /gene="infA"
                     /locus_tag="VQ516_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2511249.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            386..502
                     /gene="rpmJ"
                     /locus_tag="VQ516_000776"
     CDS             386..502
                     /gene="rpmJ"
                     /locus_tag="VQ516_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2511250.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            520..885
                     /gene="rpsM"
                     /locus_tag="VQ516_000777"
     CDS             520..885
                     /gene="rpsM"
                     /locus_tag="VQ516_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2511251.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            903..1286
                     /gene="rpsK"
                     /locus_tag="VQ516_000778"
     CDS             903..1286
                     /gene="rpsK"
                     /locus_tag="VQ516_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2511252.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            1329..2264
                     /locus_tag="VQ516_000779"
     CDS             1329..2264
                     /locus_tag="VQ516_000779"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2511253.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            2276..2662
                     /gene="rplQ"
                     /locus_tag="VQ516_000780"
     CDS             2276..2662
                     /gene="rplQ"
                     /locus_tag="VQ516_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2511254.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            2927..3193
                     /locus_tag="VQ516_000781"
     CDS             2927..3193
                     /locus_tag="VQ516_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2511255.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            3203..4540
                     /locus_tag="VQ516_000782"
     CDS             3203..4540
                     /locus_tag="VQ516_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2511256.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     IGDSVARVLEEMGLETVGTHGTTVALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            4784..5476
                     /locus_tag="VQ516_000783"
     CDS             4784..5476
                     /locus_tag="VQ516_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2511257.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGCEKMEEGSI"
     gene            complement(5579..7224)
                     /locus_tag="VQ516_000784"
                     /pseudo
     CDS             complement(5579..7224)
                     /locus_tag="VQ516_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(7214..7519)
                     /locus_tag="VQ516_000785"
                     /pseudo
     CDS             complement(7214..7519)
                     /locus_tag="VQ516_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            complement(7646..8305)
                     /locus_tag="VQ516_000786"
     CDS             complement(7646..8305)
                     /locus_tag="VQ516_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511258.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     K"
     gene            complement(8318..9439)
                     /locus_tag="VQ516_000787"
                     /pseudo
     CDS             complement(8318..9439)
                     /locus_tag="VQ516_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(10027..10803)
                     /locus_tag="VQ516_000788"
     CDS             complement(10027..10803)
                     /locus_tag="VQ516_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2511259.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            10925..11671
                     /locus_tag="VQ516_000789"
     CDS             10925..11671
                     /locus_tag="VQ516_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2511260.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            11684..12664
                     /locus_tag="VQ516_000790"
     CDS             11684..12664
                     /locus_tag="VQ516_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2511261.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            12859..13179
                     /locus_tag="VQ516_000791"
     CDS             12859..13179
                     /locus_tag="VQ516_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2511262.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            13225..13533
                     /locus_tag="VQ516_000792"
     CDS             13225..13533
                     /locus_tag="VQ516_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2511263.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            13550..14848
                     /locus_tag="VQ516_000793"
     CDS             13550..14848
                     /locus_tag="VQ516_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2511264.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQTLGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            14999..17437
                     /locus_tag="VQ516_000794"
     CDS             14999..17437
                     /locus_tag="VQ516_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2511265.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            17456..18124
                     /locus_tag="VQ516_000795"
     CDS             17456..18124
                     /locus_tag="VQ516_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2511266.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            18142..19230
                     /locus_tag="VQ516_000796"
     CDS             18142..19230
                     /locus_tag="VQ516_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2511267.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            19685..22186
                     /locus_tag="VQ516_000797"
     CDS             19685..22186
                     /locus_tag="VQ516_000797"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2511268.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLIRSKEIVAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            22380..23075
                     /locus_tag="VQ516_000798"
     CDS             22380..23075
                     /locus_tag="VQ516_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511269.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            23087..23503
                     /locus_tag="VQ516_000799"
     CDS             23087..23503
                     /locus_tag="VQ516_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511270.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(24130..24222)
                     /locus_tag="VQ516_000800"
     CDS             complement(24130..24222)
                     /locus_tag="VQ516_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2511271.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            24383..25354
                     /gene="ruvB"
                     /locus_tag="VQ516_000801"
     CDS             24383..25354
                     /gene="ruvB"
                     /locus_tag="VQ516_000801"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2511272.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            25335..25907
                     /locus_tag="VQ516_000802"
     CDS             25335..25907
                     /locus_tag="VQ516_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2511273.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            26138..26896
                     /locus_tag="VQ516_000803"
     CDS             26138..26896
                     /locus_tag="VQ516_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2511274.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            26905..27708
                     /locus_tag="VQ516_000804"
     CDS             26905..27708
                     /locus_tag="VQ516_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2511275.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            27730..28989
                     /gene="rseP"
                     /locus_tag="VQ516_000805"
     CDS             27730..28989
                     /gene="rseP"
                     /locus_tag="VQ516_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2511276.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            29002..30855
                     /locus_tag="VQ516_000806"
     CDS             29002..30855
                     /locus_tag="VQ516_000806"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2511277.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            30955..32334
                     /gene="bglA"
                     /locus_tag="VQ516_000807"
     CDS             30955..32334
                     /gene="bglA"
                     /locus_tag="VQ516_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2511278.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIHDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            32526..34334
                     /gene="glmS"
                     /locus_tag="VQ516_000808"
     CDS             32526..34334
                     /gene="glmS"
                     /locus_tag="VQ516_000808"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2511279.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
BASE COUNT        10494 a         5880 c         7579 g        10513 t
ORIGIN      
        1 aaaaagtatt gacaaatttg aaataaagcg tttttcgcac ttgcaaaaat ccgctacaaa
       61 tgttatactg agatagtctg acttataatt gttgtctctg tgtctagagg catcgaatcg
      121 aaatttatgg aggtgctttt gcgtggcaaa agacgatgtg attgaagttg aaggcaaagt
      181 agttgataca atgccgaatg caatgtttac ggttgaactt gaaaatggac atcagatttt
      241 agcaacagtt tctggtaaaa ttcgtaaaaa ctatattcgt attttagcgg gagatcgtgt
      301 tactgtcgaa atgagtccat atgacttgac acgtggacgt atcacttacc gctttaaata
      361 atcgaaaaac ttggagggat aagaaatgaa agtaagacca tcggtcaaac caatttgcga
      421 atactgtaaa gttattcgtc gtaatggtcg tgttatggta atttgcccag caaatccaaa
      481 acacaaacaa cgtcaaggat aagatagaaa ggagaaaaca tggctcgtat tgctggagtt
      541 gatattccaa atgacaaacg cgtagtaatc tcattgactt atgtttatgg tatcggactt
      601 gcaacatcta agaaaatttt ggctgctgct ggaatctcag aagatgttcg tgtacgtgat
      661 cttacatcag atcaagaaga tgctatccgt cgtgaagtgg atgcaatcaa agttgaaggt
      721 gaccttcgtc gtgaagtaaa cttgaacatc aaacgtttga tggaaatcgg ttcataccgt
      781 ggtatccgtc accgtcgtgg acttcctgtc cgtggacaaa atactaaaaa caacgctcgc
      841 actcgtaaag gtaaagctgt tgcgattgct ggtaagaaaa aataatatag gaggtaaaag
      901 tcttggctaa accaacacgt aaacgtcgtg tgaaaaagaa tatcgaatct ggtattgctc
      961 atattcacgc tacatttaat aacactattg ttatgattac tgatgtgcat ggtaatgcaa
     1021 ttgcttggtc atcagctggt gctcttggtt tcaaaggttc tcgtaaatct acaccattcg
     1081 ctgctcaaat ggcttctgaa gctgctgcta aatctgcaca agaacacggt cttaaatcag
     1141 ttgaagttac tgtaaaaggt ccaggttctg gtcgtgagtc agctattcgt gcgcttgctg
     1201 ccgctggtct tgaagtaaca gcaattcgtg atgtgactcc agtgccacac aatggtgctc
     1261 gtcctccaaa acgtcgccgt gtataatcat cgcattacac tgcttttcgt ttaagaggga
     1321 gtaactaaat gatcgagttt gaaaaaccaa atataacaaa aattgatgaa aataaagatt
     1381 atggcaagtt tgtaatcgaa ccacttgaac gtggctacgg tacaactctt ggtaactctc
     1441 ttcgtcgtgt acttctagct tctctaccag gagcagctgt gacatctatc aacattgatg
     1501 gtgtgttaca tgagtttgac acagttccag gtgttcgtga agacgtgatg caaatcattc
     1561 tgaacattaa aggaattgca gtgaaatcgt acgttgaaga cgaaaaaatc atcgaactgg
     1621 atgttgaagg tcctgctgaa gtaacagctg gtgacatttt gacagatagc gatattgaaa
     1681 ttgtaaatcc agatcattat ctctttacaa ttggtgaagg ttcttctcta aaagcgacta
     1741 tgactgttaa cagtggtcgt ggatatgtac ctgctgatga aaataaaaag gataatgcac
     1801 cagttggaac acttgctgta gattctattt atacaccagt tacaaaagtc aactatcaag
     1861 tggaacctgc tcgtgtaggt agcaatgatg gtttcgacaa attaaccctt gaaatcttga
     1921 caaatggaac aattattcca gaagatgctt tagggctttc agcacgtatt ttgacagaac
     1981 atcttgattt gtttacaaat cttactgaga ttgctaagtc aactgaagtg atgaaagaag
     2041 ctgatactga atctgacgac cgtattttag atcgtacgat tgaggaactg gacttgtctg
     2101 tgcgttcata caactgttta aaacgtgccg gtatcaatac tgtgcatgat ttgacagaaa
     2161 aatctgaagc agagatgatg aaagtacgaa atcttggacg caagagtttg gaagaagtga
     2221 aactcaaact cattgatttg ggtcttggat taaaagataa ataaaggagg aatacatggc
     2281 ttaccgtaaa ctaggacgca ctagctcaca acgtaaagca atgcttcgcg atttgacaac
     2341 tgaccttttg atcaacgaat caatcgtgac aactgaagct cgtgctaaag aaatccgtaa
     2401 aactgttgaa aaaatgatta ctctaggtaa acgtggtgat ttgcatgcac gtcgtcaagc
     2461 agctgctttc gtacgtaatg aaatcgcatc tgaaaactat gatgaagcaa ctgataagta
     2521 cacttctact acagcacttc aaaaattgtt ctcagaaatc gcacctcgtt atgctgaacg
     2581 taacggtgga tacactcgta tccttaaaac tgaaccacgt cgtggtgatg cagcgccaat
     2641 ggcgatcatc gaattagtat aaaatcatca attttgttga gtgttatgat gatggagtct
     2701 tgtgctctta gtctagctct ggtctaccgc taggatttcg gtcctagcgg gaacactcat
     2761 cataagttgg gatagtagac gcttgtttac gaaattgttt ttttcttaag aacaacttcg
     2821 taagcaggcg tttttgagta ttttcgttag aattatgcta tactatttga aaagaatcct
     2881 gtttaatgtt aaggtttctt attttaagaa gaattggagt ttacttatga aagccattat
     2941 aactgttgtt ggtaaagata aatctggaat tgttgcaggt gtttctggta aaattgcaga
     3001 attaggattg aatattgacg atatctctca aactgtcttg gatgaatatt ttacgatgat
     3061 ggctgttgta tctagtgatg aaaagcaaga ttttacctat cttcgtaatg aatttgaagc
     3121 ttttgggcaa actttgaatg taaaaatcaa tattcagagt gcagcgattt tcgaagctat
     3181 gtataatatc taggaggtca tcatggatat tagacaagtt actgaaacca tcgccatgat
     3241 tgaggagcaa aacttcgata ttagaaccat taccatgggg atttctcttt tggactgtat
     3301 cgatccagat atcaatcgtg ctgcggagaa aatctatcaa aaaattacga caaaggcggc
     3361 taatttagta gctgttggtg atgaaattgc ggctgagttg ggaattccta tcgttaataa
     3421 gcgtgtatcg gtgacaccta tttctctgat tggggcagcg acagatgcga cggactacgt
     3481 ggttctggca aaagcgcttg ataaggctgc gaaagagatt ggtgtggact ttattggtgg
     3541 tttttctgcc ttagtacaaa aaggttatca aaagggagat gagattctca tcaattccat
     3601 tcctcgcgct ttgactgaga cggataaggt ctgctcgtca gtcaatatcg gctcaaccaa
     3661 gtctggtatt aatatgacgg ctgtggcaga tatgggacga attatcaagg aaacggcaaa
     3721 tctttcagat atgggagcgg ctaagttggt tgtattcgct aatgctgttg aggacaatcc
     3781 atttatggcg ggtgcctttc atggtgttgg ggaagcagat gttatcatca atgtcggagt
     3841 ttctggtcct ggtgtggtga aacgtgcttt ggaaaaagtt cgtggacaga gctttgatgt
     3901 agtagccgaa acagttaaga aaactgcctt taaaatcact cgtatcggtc aattggttgg
     3961 tcaaatggcc agtgagagac tgggtgtgga gtttggtatt gtggacttga gtttggcacc
     4021 aacccctgcg attggagact ctgtggcacg tgtccttgag gaaatggggc tagaaacagt
     4081 tggcacgcat ggaacgacgg ttgccttggc cctcttgaac gaccaagtta aaaagggtgg
     4141 agtgatggcc tgcaaccaag tcggtggttt atctggcgcc tttatccctg tttctgagga
     4201 tgaaggaatg attgctgcag tgcaaaatgg ctctcttaat ttagaaaaac tagaagctat
     4261 gacggctatc tgttctgttg gattggatat gattgccatc ccagaagata cgcctgctga
     4321 aaccattgcg gctatgattg cggatgaagc agcaatcggt gttatcaaca tgaaaacaac
     4381 agctgttcgt atcattccca aaggaagaga aggcgatatg attgagtttg gtggtctatt
     4441 aggaactgca cctgttatga aggttaatgg ggcttcgtct gtcgacttca tctctcgcgg
     4501 tggacaaatc ccagcaccaa ttcatagttt taaaaattaa gaaaatagga gaaattttaa
     4561 gttctattta agattagatg tgtatactat aatcattaaa taaagacctc ctaatattat
     4621 ttgaaacaga taacactgaa ttagtttgaa tttgattttc atctaatatc tttatttaat
     4681 gaactcctaa actttttcat aataatctcc ttcaaaagtc gcctgtatgg gtggctttta
     4741 ttttatcatt catgatataa tagaagcaaa cggaggacgg aaaatggtaa aagtacgatt
     4801 gtatttggta cgtcatggca agaccatgtt taacacgatt ggtcgcgcgc aaggttggag
     4861 cgatactccc ttaactgctg aaggtgaacg agggattcaa gagttaggaa tcggtttgcg
     4921 agaatctgat ctacagtttg agcgcgctta ttcgagtgat tctggtcgta ccattcagac
     4981 catgggaatt atccttgaag aacttggctt gcaggggaaa atcccttatc gcatggacaa
     5041 gcgtatcaga gaatggtgtt tcggtagttt tgatggagcc tatgatggcg atcttttcat
     5101 gggcattatt cctcgtatct ttaatgtgga ccacgttcac caattgtctt atgctgaact
     5161 ggctgagggc ttggtagagg tcgatacagc tggttgggct gaaggctggg aaaaactcag
     5221 tggccgaatc aaggaaggct ttgaaatgat tgcaaaagaa atggaagatc aaggtggagg
     5281 taacgccctt gttgtcagcc atggaatgac tattggaacc attgtttatc tgattaatgg
     5341 catgcatccg catggtctag ataatggtag cgtgacgatt cttgaatatg aggacggtca
     5401 gtttagggtt gaagttgtcg gtgaccgtag ttaccgagag ctaggatgtg agaagatgga
     5461 agaaggctct atttaatcag tctagacttg cttgccatga gctagggatt tgataagaat
     5521 atcaagataa gaaaaaacag ccgagggcac tcctttcggc tgtttttgat gtggaaaact
     5581 aaagtgtaat gctattgctt ttagagattt tcataaacaa gagcaaggaa cctactgtta
     5641 gaacagtcag gatagttgac aaggctgcgg ctacaccgta atttcctctg agaacctctg
     5701 tataaatagc tacagtcatt gttcttgttt tgacattgta gaggaggata gaagtagaga
     5761 gttttgaaat cattgtgacc caagataaga tggctccaga aatgatacca gatagcatca
     5821 ttggagttgt aatcttagca aaggtattga gacgactact tcctaagctt tcagcagctt
     5881 cttcaatact tggtgctatt tgttgtaagc tagcaacaga tgagcgaata gtataaggta
     5941 atcttctgac agatagagac ataatcaaga tgaaagcagt ccctgtaatc ataagaaatc
     6001 cacttccaaa tagaccagta ttgaaggaag aaatgaaggc aatccctaga acggttcctg
     6061 gtacaatata aggtaccata ctgaggctgt caattaagtt tgtaaacaaa ttccgttttc
     6121 taacggctag gtaggagata aatgtcgcaa atagaacaac tagaactaag gcaatcaaag
     6181 ggatacgaat ggtattgaaa atagcagatc ccatacgatt gaaagctacc ttgtaactgt
     6241 ttggagaata acctttaaca gataccatac ctgatgtttt taggaaagag gtataaatta
     6301 agtagatttg aggtaaaaca gagataaaga taattccgta gactgttgca taaatggcag
     6361 ctatttttcc ttttgtagtt tttttaggct caattggatg gagcagattc atgctgaaac
     6421 tgtagcggtt tgcaatgtgt ttttggataa ggaaaattgc caaggcaatg ataatcgcca
     6481 taattgcaaa agcagaattt cctccaacct cgctaataaa ttgggtataa atcaggacag
     6541 ggaaagtccg atatccttcg tcaatcaaca taggcgttcc aaagtctgag aatgctctca
     6601 taaatacaag caaggagctg ctagtaaggt tggaactagg agaggtaaaa caaccgttac
     6661 gatacgctta aatccgaagg accccatgct ttcagctgct tcaagtagag aattgtcaat
     6721 actgttcatt gttccagcaa catatagaaa taccagtggg aatagttgca gtgtaaagac
     6781 aagtacaatt cctttgaatc cataaatatc gatagctgga agatgaaggg catttgtcaa
     6841 aaatttagtg atgacctcat ttcgtcccag caagagaatt caggagtagg ctcctacgaa
     6901 aggagctgac atggaagcaa tgataatcaa tatttgtaga aatttcttcc ccttgaagtc
     6961 atacatagag aagagataag ctaatagggt tcctacaact aaggaagtga tagtagcggt
     7021 aatggaaacc ttgaaactgt tgactagtgt ctcagagtag taggctttac taaagaaagt
     7081 gacaaaatta gctagtgaga attgtccttc atgtataagt gcttgcttga gcacggtaac
     7141 gataggataa acgagaaaga caagataggt aagaaagatg aagaaagagg aagctgtcca
     7201 aatatttagt tttttacatt ccatggttga ctccttttat caggttttgg gaaccatctg
     7261 cagaaaagat gtttaatttt tgcgtattga ttcgtagacg aatacgattg cctttttgta
     7321 gttcttcttc aaaagttgat tcttcactaa cttaaatttt tgaggcaaaa cctgtctcaa
     7381 tgaaatactc cgtatttagt ccaagataga cgctatcgct aatagttcct tcgatatctc
     7441 cagattcatc tttgataaac tcttcgggac gaatgcttac atgaatagct tgctcctcaa
     7501 cctgatcaag agctggcatt cgaagggcat agccatctga aaagacgata taagcgccgt
     7561 cgctccgttt ttcaagattg gcagggataa tatttgtgcg tccgataaag gttgccacaa
     7621 actcattagc tggtttatga tagagttatt ttggtcggcc gatttgttgg atcaccccat
     7681 ctttcataac agcaatttgg tctgaaatag ccatggcttc ttcttggtcg tgggttacat
     7741 aaacagttgt aattcccact tcgtgttgga tttctcggat ggcttgacgc atatccaagc
     7801 gaagtttggc ctccagatta ctaagtggct cgtccatgag gagaacactt ggattaaccg
     7861 ctaaggcgca tgccaaggtg acacgttgtt gttgtccacc actgagttta tcgggctttc
     7921 gatccgcata ttgagcaatt tgcatgagtt caagatactt gttggtctgt tgaatcaatt
     7981 cttcttttgg aaccttcttt tgcataagac caaaagcaac gttgtctcgg acagtcaaat
     8041 gtgggaaaat agcgtagttt tggaaaacca tcccgatatt gcgtttgctg ggttccatat
     8101 tattgatttt tgtatcatcg aagtaaaatt ctccaccttc gatactgttg aaacctgcaa
     8161 tcatacgaag aagggtcgtt ttcccacatc ctgaagctcc aagaagggta aagagacttc
     8221 cttttggaat tgtaatgttc aaattctcaa taacagggac atcgtggtag atttttttgg
     8281 cgttaataat ttcgatctca ctcatagtga acctctttta ctgtttagat tggatatctg
     8341 taaagacttc gttgtatttc ttaacgatat ctgatttatt cttgatgaca taatcataat
     8401 cttcagtgag tgttttgatt ttgtcaattg gtttcatgtt ttcgcttgtt ttagcatttt
     8461 tacgaacagg acggttagta gtggttgtac caagtgtatc ttgtacttct tgagagataa
     8521 taaaatcgat aaatttcttg gcattttcca tatttttaga ttttttaacg atagcagcac
     8581 tagcaggtag gaagacggtt ccttcttttg gatagactac cttaatgtta gctccgtcat
     8641 ttaagagttt aactgctgga tcttcataag agagactaac agtcatttct ccatcagcga
     8701 ctgctttata gacaccagat gaacttaaac cgattttata gtatattgaa actagaatag
     8761 tacacatcta cttctaaaac attgttagaa atcgatttga ctgtcctgat taatttgtcc
     8821 tgttcttatt tcattttact ataccatcaa taagtgtgaa aagatctttt acataagacc
     8881 aagccttatc atctttgtaa ccaccttgag cttgtagcat atttgttaat tgagcaaagg
     8941 cgctagaaga gtttgctggg tcagcagttg cgatttttcc ttttagttca ggtttgagaa
     9001 ggtcgctata tccttcgatg ttcatgcctt tagttaaatc agggtggacg attaaaacac
     9061 taccatctag tgtataagga gtagagaagc cagttgtgtt ttgatattct ttgataacat
     9121 tatcattttc ttttgaaata tagttttcaa agagttctcc gtgggtagca tattgtgtat
     9181 aagaaccacc aaagataata tcagctacag gaacttcttt ttctgactct agttttttga
     9241 aaagttctcc agtaccagct tgaatcagtt ctattttgat accatatttt tcttcaaagg
     9301 caggaatagt tgctccaatt aagccttctg agtttggtga ataaacgact agcgaaccac
     9361 cgtctccttt atcagatgaa ctgtcatcgg cagattcatt agaagaacaa gcagcataat
     9421 acatccattt ctttttcatg atggatacct ccgttgtgtt atttaagttt attttaaaac
     9481 aatgtaagcg tttttaaaac ctacaattct attctatagt gtattgaatc tataacagta
     9541 cacattgact gctaaaatat ttctataaat taatttgact ttcctgatag agatgttcac
     9601 atcttatttc aattcactat attagagtaa aattctctac aaaaaaaaag aatagcctat
     9661 tttactattt ttccgagtga tttcaattcc tttggggaaa tatggagata ctttttaaat
     9721 cctgacaaat ggttgtttct ttttctaaat cggtgatact gtatcggaga atgcgcgtga
     9781 ggtcacaaag gctgcgatag agcttctatg gagaatttct ttttggagag attttttaaa
     9841 ggaatgagac atccgctacc tccttggaag gtttttttgt gataagtata gcagtttaaa
     9901 aattctagag caaggtaaaa tagaaaaggt ttggctaaaa ttttttagct aaaccttttg
     9961 aaaagttaag tatgtattat agatgcttgt tatctgcaaa tagctagagc atttcaagga
    10021 aataaactag aaataacaat taatatggtg gttcagaaat accttttgat aatcaataag
    10081 atcttcagga tgaagtgcgt tgattccatc catttcatat ttccgattta ataaacgata
    10141 tttgttttca ccaaattgat gaaaagggag tagttgaact tggtcgatat ttaatgagtt
    10201 aaatagagta gcgaattttt ctgcatcctc taaactattg ttaaaattag gaataactgg
    10261 gattcttaaa acgatagttt tattttgtga aaaagcataa tgaatgtttt taataatcat
    10321 ttgattaaaa accccagtca cttttttatg ttttatagaa ttataatgtt ttaggtctgt
    10381 gtagataaaa tccacatatt gaattaaatc aataaatttt tcatgatcaa caaaggcagt
    10441 agtttcaatg gcagtgtgta tgtgatgttc tttagctgat tttaagatag ctttagcaaa
    10501 ttcaaactga gcaaatattt cacctcctga taaagttaaa cctccaccgg attcttcgta
    10561 aaattctttg tcttttaata cctctgtaat aatttcttct acagtctttt cttcaccgac
    10621 taaggtgaat ttctctcgtt gagcatcttt cattttttca ggtttcattc tttgagattc
    10681 aggattagaa caccatggac agcgcagagg acatcctttt aaaaaaacag ttgtacgaat
    10741 acccgggccg tcatgaattg aaaagtgttg aatattaaaa ataattcctt tagatatttc
    10801 cataagagtt cctcctcttc ataagtctat cctatcacaa aaacgaaaga aaaacaatta
    10861 cgaacgaaag gttaatttgt tgttttttgc tttttatttt ggtagaataa aacggaggtg
    10921 taaaatgaaa agattggaac aaattattaa attagtatca gaatatgaaa agatcgacgt
    10981 taatacatta tcggaaaaat taaatgtatc gaaagtaacg attagaaaag atttagataa
    11041 attagagtca aaaggtttat tacacagaga gcatggatat gctgtattaa atagtggaga
    11101 tgacttaaat gtacgtttgt caattaatta tgaaattaag agaaaaattg ttcaggaagc
    11161 agtaaaattg gtgtcagata atgaaacgat aatgatagaa tctggatcga cctgtgcttt
    11221 acttgctgag gaaatttgca agcaaaaaag aaatgttacg attgtaacaa attcattttt
    11281 tatagcaaat tttgtgagag cttatgattc atgtcgtatt attgttcttg gtggagagtt
    11341 tcagaaggat tcacaggtta ctgtgggacc tttattaaaa gaaatgatac agacttttca
    11401 tgtgcatcaa gcttttgttg ggacagatgg atacgataaa gagatgggct ttacaggaaa
    11461 agatttaatg cgcagtgagg tagttcaata tatttcagca gcgtcggata aagtcattgt
    11521 cctaactgac tcaagtaaat ttgataaaag gggtacagta agaagatttg ctttaagtca
    11581 agtctatgaa gtaataacag acgaaaaact ttctaaacaa aatatagcta cattagaaaa
    11641 tgctgggata atggttaagg tagtttcgta agaggttaag tgtatgaatc aagataggaa
    11701 taaactgctc tctaaaattg cttatctgta ttatattgaa aacttaaatc agtcacaaat
    11761 agcagcaaaa ttaggaattt atagaacctc tattagtaga atgttaacag aagcaaggaa
    11821 tgcaggaatt gttaaaattg aaatagagaa ttttgatacc aatatgttta agttggaaaa
    11881 ttatgtaaaa gaaaaatatg gtctggaaag tttagaaatt attccaaatg aatttgatga
    11941 taatccaaca attttatctg aaagaatttc tcaagttgca gcaggtgtcc ttaggaatct
    12001 aattgatgat aatatgaaaa ttggcttttc ttgggggaaa agtttaagta atttagtaga
    12061 tttaattcac agtaaaagtg tccgaaatgt tcacttctat cctctagcag gtggtcctag
    12121 tcacatacac gctaaatacc atgtgaatac actgatttat gaaatgtcta gaaaatttca
    12181 tggagagtgt acatttatga atgcaacgat tgtgcaagaa aataaattgt tagcagatgg
    12241 tattttgcaa tcaagatatt ttgaaaattt gaaaaatagt tggaaagatt tagatatagc
    12301 tgtagtcgga attggtgatt ttagcaataa aggaaagcat caatggttag acatgcttac
    12361 agaggatgac tttaaagaat tgaccaaagt taaaactgta ggagaaattt gttgtcgatt
    12421 ttttgattca aaaggtaaag aagtctatga aaacttacag gaaagaacga tagcaatctc
    12481 tttggaagat ttaaaaaata ttcctcaaag tttagctgtt gcttacggtg atacgaaagt
    12541 atcttcgatt ctttctgtct tgcgtgctaa tttagtaaat catttgatta cagacaaaaa
    12601 tacaatttta aaagttttgg aagaagatgg ggatttgact tttagagaga ttctaggtga
    12661 gtgaaaatga tagactgatt cagtttatcg tttttctttt tagttgattg cacatttgtg
    12721 cttatataaa caaaaatagt ttatctgttg tttttggatt gacaacttta ttatgtagtt
    12781 gtattctata gttacaaaag aaaattttaa aatttcaaat gaaaaaagct ttttacatag
    12841 tgaaatgagg aggaatttat ggaaattatt gttccagatc aaattatcat gggtttaatt
    12901 ttatatgctg gtgatgcgaa acaacatatt tataaagcgt tagattacat aaaaaatggt
    12961 acatgtgaac ggtgtgaaga agaaatccag ttagctgatg ccgccttatt agaagcccat
    13021 aatctacaaa caaaattctt ggcacaagaa gcgtctggta caaagacaga aattacagct
    13081 ctctttgttc attcacaaga tcatctcatg actagtatga cggagattaa tttaatcaaa
    13141 gaaattatta gtttgagaaa agaacttcat aaaaaataat actagagtat tatcattgtt
    13201 attaacatag aggaggaaaa cataatggtg aagattggtt tgttttgtgc agcaggtttt
    13261 tctactggta tgcttgtaaa taatatgaaa attgcagcgc aatctagtgg agttgaggca
    13321 gaaatagagg cgttttctca gtctaaatta gcggattatg cgccaaatat agatgttgca
    13381 ctattgggtc cacaagttgc ttatacatta gataaatcaa aagaaatttg tgataagtgt
    13441 gatgttccga tagctgttat tccgatgatg gactatggta tgttagatgg gaaaaaagta
    13501 ttagatttgg ccctatcttt gattagtggg taagaaaagg agatttatta tgtcaaagat
    13561 ggatgttcag aaaatcattg caccgatgat gaagtttgtg aatatgcgtg gcattatagc
    13621 tctaaaagat gggatgttag caattttgcc attgacagta gttggtagtt tgttcttgat
    13681 tatgggacaa ttgccgttcg aaggattaaa taagagcatt gctagtgttt ttggagctaa
    13741 ttggacagag ccgtttatgc aagtatattc aggaactttt gctattatgg gtctaatttc
    13801 ttgtttttca attgcctatt cttatgctaa gaatagcgga gtagaggctt taccagctgg
    13861 agttctatct gtatctgcat tctttatttt gctaagatca tcttatatcc ctaaacaagg
    13921 tgaggcgatt ggggacgcta ttagtaaagt ttggtttgga ggccaaggaa ttatcggtgc
    13981 tatcattata ggtttggtag taggaagtat ttataccttc tttataaaga gaaaaattgt
    14041 tattaagatg ccagaacaag ttccacaagc tattgccaaa cagtttgaag caatgattcc
    14101 agcatttgta attttcttat cttctatgat tgtatatatt ttagcgaagt cattgactaa
    14161 tggcggaaca ttcatagaaa tgatttattc tgctattcaa gttccgttgc aaggtttaac
    14221 tggatctttg tatggtgcta ttggaattgc attctttata tcatttttgt ggtggtttgg
    14281 tgttcatggg caatcggtag taaatggagt agtgacagct ctgcttttat ctaatcttga
    14341 cgctaataaa gctatgttag cctctgctaa tctatcatta gaaaatggtg cacatattgt
    14401 tactcaacaa tttttagatt catttttaat tctatcaggt tcagggatta cgtttggtct
    14461 tgtagttgcc atgctttttg cagcaaaatc aaaacaatac caaaccttag gaaaagttgc
    14521 agcttttcca gcaatattta acgtaaatga gccagttgta tttggatttc cgattgtcat
    14581 gaatccagtt atgtttgtac ctttcattct tgttcctgta cttgcagctg tgatagtata
    14641 tggagctatt gcaacaggtt tcatgcagcc attctcaggg gtaacattgc cttggagtac
    14701 accagctatt ttatcaggat ttttggtggg tggatggcaa ggagttatta ctcagctggt
    14761 gatattagcg atgtctacat tggtttattt tccattcttt aaagtacagg atcgtttagc
    14821 ttaccaaaat gaaatcaaac aatcttagag gtatttgtgt gttactgtta aactcacaca
    14881 tttgtgctaa aaattagaga gttaaaattt ttctagttaa aagcttgaaa atttctataa
    14941 aaatcggtat tatattttcg aaagaaataa aaatattttc gaaagaaagg tggttacgat
    15001 ggtaaataca gaagtagcaa gaacaacaat caagacagaa tattttggca gccttactga
    15061 aaggatgaac aaatatcgag aagatgtttt aaataaaaaa ccttatattg atgctgagag
    15121 agcagttcta gcaacacgcg cctatgaacg atacaaggaa caacctaatg tcctaaaacg
    15181 tgcatatatg ctgaaagaaa ttttggaaaa tatgactatc tatattgaag aagaatctat
    15241 gattgcggga aatcaagctt cttccaataa agatgctcct atttttccgg aatatacgct
    15301 agaatttgtt ctcaatgagt tggatctttt tgaaaagcgt gatggagatg ttttctatat
    15361 tacagaagaa acaaaagaac aacttagaag tattgctccg ttttgggaaa ataataattt
    15421 acgtgctaga gctggtgcct tattacctga agaagtgtct gtttatatgg aaacaggatt
    15481 cttcggtatg gaaggtaaga tgaattctgg agatgctcac ctagcagtta actatcagaa
    15541 acttttgcaa tttggtttaa gaggttttga agagcgggct cgtaaagcaa aagtagctct
    15601 agatttaaca gatccagcaa gtattgataa atatcatttt tacgactcta tatttatcgt
    15661 aatcgatgct attaaagtat atgcaaagcg ctttgttgct cttgctaaaa gtttagccga
    15721 aaatgcaaat cctaaacgta agaaagaatt acttgagatt gcagatattt gctctagagt
    15781 cccatatgaa ccggcaacta cttttgcaga agctattcaa tcagtttggt ttattcaatg
    15841 tattttacaa attgaatcta atggccactc tctttcatat ggccgttttg atcaatatat
    15901 gtatccatat atgaaggctg atttagaaag tggtaaagaa acagaagata gcattgttga
    15961 acgtctgaca aatctttgga ttaagacaat tacaatcaat aaggttcgca gtcaatcaca
    16021 tacattttct tcagcaggaa gtcctttata tcaaaatgtt acaattggtg gacagactcg
    16081 agataagaag gatgctgtta acccattatc ttatttggta ttaaaatcag ttgcacaaac
    16141 ccatctaccg caacctaatc taactgtacg ttaccatgca ggtttagatg ctcgtttcat
    16201 gaatgagtgt attgaagtga tgaaacttgg ttttggtatg cctgcattta ataatgatga
    16261 gattattatt ccttctttta ttgcaaaagg agtattggaa gatgatgctt atgattacag
    16321 tgccattgga tgtgttgaaa cggcagttcc agggaaatgg ggctatcgtt gcacaggtat
    16381 gagttatatg aacttcccta aggttctact tatcacgatg aatgatggaa ttgatccggc
    16441 ttcgggtaaa cggtttgcac caagctttgg tcattttaag gatatgaaga acttttctga
    16501 attagaaaat gcttgggata aaacactaag atatttgaca cgaatgagtg ttattgttga
    16561 aaattctatt gatttatcat tggaacgaga agttcctgat attctatgtt cagcattgac
    16621 tgatgattgt attggtcgtg gaaaacacct taaagaaggt ggagcagtat atgattatat
    16681 atcaggattg caagttggaa ttgcaaattt gtcggattca ttagctgcaa ttaaaaaatt
    16741 ggtgtttgag gaagaacgta taagcccaag tcagctttgg catgcactgg aaacagatta
    16801 tgccggagaa gaaggtaagg tcattcaaga aatgttgatt catgatgcac ctaagtatgg
    16861 taatgatgat gattatgctg acaaattggt tactgctgct tatgacattt atgttgatga
    16921 aattgctaaa tatcctaata cacgttatgg aagagggcct attggaggaa ttcgttattc
    16981 aggaacatct tctatctcag ccaacgtagg gcagggacgt ggaacattag caactccaga
    17041 tggacgcaac gcgggtacac cgttagcaga gggttgttca ccatcacata atatggatca
    17101 acacggccct acatctgttt taaaatctgt ttcaaaatta ccaacagatg aaatcgtagg
    17161 tggggttctc ttaaatcaga aagtaaatcc tcaaacgtta gccaaagaag aagataaatt
    17221 aaaactaatt gctttgttac gaacattctt taatcgttta catgggtacc atattcaata
    17281 caatgttgtt tccagagaga cgttgattga cgctcagaaa catcctgaaa aacacagaga
    17341 cttaattgtt cgtgttgcag gatactctgc attcttcaat gttctttcta aggcaaccca
    17401 agatgacatt ataggacgta ctgagcatac tttgtaaaat aaagaggttc tttttatgga
    17461 atttatgctt gacacattaa atttagatga gattaaaaag tggtctgaaa ttttgccgct
    17521 agctggggta acttcaaatc ccactattgc aaaaagagag ggttctatta atttttttga
    17581 acgaatcaaa gatgtaagag aattgattgg ctctacaccc tctattcatg ttcaggtgat
    17641 ttctcaagat tttgaaggca tcttaaagga tgctcatgaa attcgaagac aagcaggaga
    17701 tgatatattt atcaaagtac ctgttactcc agctggatta cgtgcaataa aggtgctaaa
    17761 aaaagagggc taccatatca ctgcaacagc tatttataca gttattcagg gattattagc
    17821 tatcgaagta ggagcggatt acctagctcc atattataat agaatggaaa atctgaacat
    17881 tgattcaaat tctgtcattc gtcaattagc tcttgctatt gatagacaga actctcctag
    17941 taagatttta gctgcatcct ttaaaaatgt agcacaagta aataatgctt tagctgcagg
    18001 tgcgcatgct gttacagcag gagcagatgt ttttgaatca gctttcgcca tgccatctat
    18061 ccaaaaggcg gttgatgatt tttctgacga ttggtttgtt actcaaaata gtcgttccat
    18121 ttagatagag aggaaataca tatgagaatt tttgctagtc cttctagata tattcagggg
    18181 gaaaatgcct tgtttgaaaa tgccaaatca attttggatt tgggaaatta ccctattcta
    18241 ttatgcgatc agttggttta tgatattgtt ggaaaacgat ttgaagatta tctacatagg
    18301 tatggtttcc atattgttct ggcgctattt aatggtgaag cttctgacaa tgaaatcaat
    18361 cgagttgttg ccttggctga gaaagaaaat tgtgatagta ttatcggtct tggtggggga
    18421 aagacgattg atagcgcaaa agctattgca gatttgattg aaaagcctgt tattattgct
    18481 ccaacaattg catcgaccga cgcacctgta tctgctttat ctgttattta tacagatgaa
    18541 ggtgcatttg atcattatct attttattct aaaaatccag atttagtttt ggttgataca
    18601 aaagttattt cacaagcccc taagcgttta ttagcgtctg gtattgcaga tggtttagca
    18661 acttgggttg aggcgcgtgc ggttatgcag gcaaatggaa aaactatgtt gggacaacag
    18721 caaacattgg ctggagttgc aattgcgaag aaatgtgaag aaacgctgtt tgcagatggt
    18781 ttacaggcta tggcagcttg tgaagctaaa gtggtgacac cagcattaga aaatattgtt
    18841 gaagctaata ctttattgag tggcctaggt tttgaaagtg gaggattagc tgcggcgcat
    18901 gcaattcata atggttttac tgcattgaca ggtgacattc atcatttaac acatggtgaa
    18961 aaagtagctt atggaacttt agtacaacta ttattggaaa atagacctaa agaagaactt
    19021 gataagtata ttgagtttta caaaaaaatt ggtatgccaa caactctaaa agaaatgcat
    19081 ttggatcaag ttggatatga tgatttaata aaagttggta aacaagcaac tatggagggt
    19141 gagacaattc atcagatgcc gtttaagatt tcgccttcag atgttgctca agctattatc
    19201 gctgtagatg cctatgtaaa ttcaaaataa acaataagga ctactgtttt ccaaatggta
    19261 gtcttttatt gatcctcgta ttgaattcta tagcagattg aaataagatg agaacaaatc
    19321 gattgggaaa gtaaaattaa tttctataag tattttagca attgtttcgt actatttcag
    19381 attcagtcca ctatacgttc ttcataaatc aaaaagcgac ataggttgtc ggctatttat
    19441 tgtgaataca ttaattagca ttccagtttt atcttcggtc taaaataagt attttgtgct
    19501 atacgagata agtttcttga cttactcctt gatttactgc ataacaatgg gataaaaagt
    19561 gggagataga gcaattcata gtcatcaaaa ttaatgagat acagtataca gtttttcctt
    19621 taaacacatt tcaaattccc tcaaaaatgg tataatagta acatcacaaa attggagaga
    19681 gaccatgagt ttttacaatc ataaagaaat tgagcctaag tggcagggct actgggcaga
    19741 acatcataca tttaagacag gaacagatgc atcaaaacct aagttttatg cgcttgatat
    19801 gttcccttat ccgtctggag ctggtctgca cgtaggacac ccagaaggtt atactgcaac
    19861 cgatatcctc agtcgttaca aacgtgcgca aggctacaat gtccttcacc caatgggttg
    19921 ggatgctttt ggtttgcctg cagagcaata cgctatggat actggtaatg acccagcaga
    19981 atttacagcg gaaaacattg ccaacttcaa acgtcaaatc aatgcgcttg gattttctta
    20041 tgactgggat cgtgaagtca acacaacaga tccaaactac tacaagtgga ctcaatggat
    20101 tttcaccaag ctttacgaaa aaggcttggc ctatgaagct gaagtgccag taaactgggt
    20161 tgaggaattg ggaactgcca ttgccaatga agaagtgctt cctgacggaa cttctgaacg
    20221 tggaggctat ccagttgtcc gcaaaccaat gcgccaatgg atgctcaaaa tcacggctta
    20281 cgcagagcgc ttgctcaatg acttagatga actagattgg tcagagtcta tcaaggatat
    20341 gcaacgcaac tggattggta aatcaactgg tgccaatgta actttcaaag taaaaggaac
    20401 agacaaggaa tttacagtct ttactactcg tccggacaca cttttcggtg cgactttcac
    20461 tgttttggct cctgaacatg aattagtaga tgccatcaca agcacagagc aagcagaagc
    20521 agtagcagac tacaaacacc aagccagcct taagtcagac ttggctcgta ccgaccttgc
    20581 caaagaaaaa actggtgtat ggactggtgc ttatgccatc aatcctgtca atggtaagga
    20641 aattccaatc tggattgcgg attacgttct tgctagctat ggtacaggtg cggtaatggc
    20701 tgtgcctgcc cacgatcaac gtgactggga atttgccaaa caatttgacc ttccaatcgt
    20761 cgaagtactt gaaggtggaa atgttgaaga agctgcctac acagaagatg gccttcacgt
    20821 taattcagac ttcctagatg gactcaacaa agaagacgct attgccaaga ttgtggcttg
    20881 gttggaagaa aaaggctgtg gacaagagaa ggtaacctac cgtctccgcg actggctctt
    20941 tagccgtcaa cgttactggg gtgagccaat cccaatcatt cattgggaag atggaacttc
    21001 aacagctgtc cctgaaagtg aattgccact tgttttgcct gtaaccaagg atatccgtcc
    21061 ctcaggtact ggtgaaagtc cactagctaa cttgacagac tggcttgaag tgactcgtga
    21121 agatggtgtc aaaggtcgtc gtgaaacaaa tactatgcca caatgggcag gttcaagctg
    21181 gtactacctc cgctatattg acccacacaa tactgagaaa ttggctgacg aggatctgct
    21241 caaacaatgg ttgccagtag atatctacgt gggtggtgcg gaacatgctg tacttcactt
    21301 gctttacgct cgtttctggc ataaattcct ctatgacctc ggtgttgttc caactaagga
    21361 accattccaa aaactcttta accaaggaat gattttggga acaagctatc gtgaccaccg
    21421 tggggctctt gtggcgactg acaaggttga aaaacgtgat ggttctttct tccatgtgga
    21481 aacaggggaa gagttggagc aagcgccagc caagatgtct aaatcgctca agaacgttgt
    21541 caacccagac gatgtggtgg aacaatacgg tgctgatacc cttcgtgtct atgaaatgtt
    21601 catgggacca ctcgatgctt cgattgcttg gtcagaagaa ggtctggaag gaagtcgtaa
    21661 gttccttgac cgtgtttacc gtttgattag aagtaaggaa attgttgcgg aaaacaatgg
    21721 cgctcttgac aaggtttaca acgaaactgt taaagctgtc acagagcaaa ttgattccct
    21781 caaattcaac acagccattg cccaacttat ggtctttgtc aatgcggcta acaaggaaga
    21841 caagctctat gttgactatg ccaaaggatt tatccaattg attgccccat ttgcacctca
    21901 cttggcagaa gaactctggc aaactgttgc tgcaacaggt gagtcaatct cttacgtgac
    21961 ttggccaaca tgggacgaaa gtaaactggt tgaagacgaa atcgaaatcg ttgtccaaat
    22021 caaaggtaaa gtccgtgcca aactcatggt cgctaaagac ctatcacgtg aagaattgca
    22081 agaaattgct ctagcggatg aaaaagtcaa agcagaaatt gacggtaagg aaatcgtgaa
    22141 agtgattagt gtaccaaata aattggttaa tattgttgta aaataaccaa tttatttgct
    22201 gatttccaac ctcaaacagt ttcccaaact gtttgagcca actaaactcg ttaatatcgt
    22261 tgtgaaataa ggtaggaatc cttcagagta gaatctggag gattttttga atcttcttat
    22321 gaaagtatgg tatactatgg gcaactataa agtttgaaaa gtgaaaaaag gagaaagcta
    22381 tgccagtaaa tgaatatggt caaatgattg gggagtcaat ggaaggttat acaccaggtg
    22441 aactgccttc tattgatttc ttagaagggc gttatgctag gatagaggct ctttcagtgg
    22501 aaaagcatgc ggaggatttg ctagctgttt atgggcctga tacgcctcag gagatgtgga
    22561 cctacctttt tcaggagcca gtagcagata tgggggagct ggttagcctt ttacatcaga
    22621 tgttggctcg taaggaccgt ttttactatg caatcataga caaggcgact ggtaaggctt
    22681 tgggatcttt ttctctcatg cgcattgacc agaataaccg agtaatagaa gtcggagcag
    22741 tcactttttc tccagagcta aggggtacac ggataggaac tgaagctcag tatctcctag
    22801 ctcgctatgt ttttgaggag ctaaactatc gtcgctacga gtggaaatgc gatgccttaa
    22861 atctgccatc cagacgagct gcggagcgtt tgagctttat ttatgaagga accttccgtc
    22921 aggcagtggt ttataaggga cgtacgagag atacggattg gttgtctatg attgataagg
    22981 actggcctca agtcaaagat cgtttggaaa catggttgcg tcctgaaaat tttgataaaa
    23041 atggacagca gtacaagagc ttgagagaac tttaagaggt gttgagatga ttactattaa
    23101 aaagcaagaa attgtcaagc tagaggatgt tttacatctc tatcaggctg tcggttggac
    23161 aaattatacc catcaaccag agatgctgga gcaggcctta tctcattcat tagtaattta
    23221 tctggcactt gatggtgatg ctgtggtggg cttgattcgt ttggttggag atggattctc
    23281 atcagtattg gttcaggatt taatcgtttt accaatctat cagcgtcaag gaattggtag
    23341 tgccttgatg aaagaggctt tagaggatta caaagatgcc tatcaagtcc agctggtgac
    23401 agaagagaca gaaagaacct tgggcttcta tcgttctatg ggctttgaaa tcttatccac
    23461 ctataattgt atagggatga cttggatgaa tcgtaaaaaa taacaaaact tgttttttct
    23521 taagcaaaat ttaaggatgg tctagtatta tatagtcatt aaataaagac ctcctaactt
    23581 tatttaataa aatcctaaac ttttcttttt cataataatc tcccttaact ccacccaatc
    23641 aggtggagtt ttttagctct atttcaggct tttggggact attctaaaaa taatttttcg
    23701 atatttttcg gtatttttcg gattttggtc ggggaattgg cggggacttt tttagcgaat
    23761 ataactaaga aataggtctg tggtcgcttc agctagttca aactcaattt gattgtaagt
    23821 gactgttaat tgaagaactg gaactactgt aataatgatt ttgcttgtct agtagtggcg
    23881 tattaaatga gaagtgacat gtaagattgt ctcttcgttt acgaaatcaa aatttctatg
    23941 aaattgatag aatccctaac gttctcttaa tcttaaccgt cttgtcgtac agcggttgaa
    24001 gaaaatgtaa acctttgaca aaattcgtga actatgggat aataaaaagg aattgagtac
    24061 tagttctata tcataggcta gaaaagaccc caagctcacg accaaataag cttgaggtct
    24121 tttttgacac tatttgtcct tgtttagcca tttatcgact atacgaagaa cgacaccgac
    24181 cacaattggt ccgatgatag ttttgagtac taattccatc atgggctatc tcacctcctt
    24241 tcgtaggcgg tgtagcagtg tcgtagaata ttataccaca taagtgctaa acttaggagt
    24301 cacccaatca ggtggctttt ttgtttgtgg cttggttttt tgatataata gagccatgag
    24361 tagaatttta gataatgaga taatggggga tgaggagtta gtagaacgca cgctccgtcc
    24421 tcagtattta cgtgaatata tcggacagga taaggtcaag gaccagctac aaatctttat
    24481 tgaagctgcc aaaatgcggg atgaagcgct ggatcatgtg ctcttatttg ggcctccagg
    24541 tctcgggaaa acgaccatgg cctttgttat tgccaacgaa ctgggagtca atcttaagca
    24601 gacgtcgggt ccagtcattg aaaaagccgg agatctggta gctattttga atgagttaga
    24661 gcctggggat gtacttttta ttgatgagat ccatcgtttg ccaatgtcag tggaagaggt
    24721 gctttatagt gctatggagg acttctacat agatattatg attggggctg gtgagggtag
    24781 tcgtagtgtt catttggagt taccaccttt taccttgatt ggtgcgacga ctcgggctgg
    24841 tatgctctcc aatccgctac gggcacgttt tgggattaca ggccatatgg agtattatgc
    24901 ccatgctgac ttgacagaaa ttgtcgagcg gacggcagat atttttgaga tggaaatcac
    24961 tcatgaggca gcatctgagt tggccctacg tagtcgtggg acccctcgta ttgccaatcg
    25021 tctcctcaag cgcgtgcgcg attttgccca gataatgggg aatggggtaa ttgatgatat
    25081 tattaccgat aaggctttga ctatgctgga tgttgaccat gaaggtttgg actatgtgga
    25141 tcaaaaaatc cttcgtacca tgattgagat gtacagtgga ggacctgttg gtctaggaac
    25201 tctttctgtg aatatcgccg aagagcgtga gacagttgaa gatatgtatg agccttactt
    25261 gattcaaaaa ggttttatca tgcggacacg gtctggacgg gtggcgactg ctaaggcata
    25321 tgagcactta ggttatgaat acagtgaaaa ataagcaaga aatcttagag gcttttagag
    25381 aaaatccaga tatgatggcc attctgacga tcatccgaga ccttggtctg aaagactcgt
    25441 ggttggcagc aggttctgtc agaaatttca tttggaatct tttgtcagac aaatcccctt
    25501 ttgatcatga aacagatata gatgtgattt tctttgatcc agatttttct tatgaggaaa
    25561 ccttattact ggagaaaaag ctgagagagg attttcctca gtaccagtgg gaattgaaaa
    25621 atcaggtcta tatgcatcag cacagtcctc acactgcttc ctataccagt tctcgtgatg
    25681 ctatgagtaa gtatccagaa cggtgtacgg cagttggact gcgcttgaat gaagaatcag
    25741 attttgaact ctatgcacct tatggtttgg aggatatttt gaattttcaa gttcgtccaa
    25801 ctcctcattt cttagaaaat gaagaccgaa tggaactcta tcaaacacgt ttatccaaga
    25861 aaaattggca ggagaaatgg aaaaatttga tttttaaaaa tacttaagga aactttaagc
    25921 tagggagtgt acactaagtt cataagttaa gaagacctta acttaaactc ctaaaacttt
    25981 ttcataataa tctccctata aaaataaagt cgcccaatca ggcggcttat ttttttgaaa
    26041 aatgggcatg gtgcctgaga ataaatagct tagtgataga agaaaatggg gaaatatggt
    26101 ataatgaaac gatagatttt tgaataggaa taagatcatg tttggatttt ttaagaaaga
    26161 taaggctgtg gaagtagagg ttccgacaca ggttcctgct catatcggca tcatcatgga
    26221 tggcaatggc cgttgggcta aaaaacgtat gcaaccgcga gtttttggac ataaggcggg
    26281 catggaagca ttgcaaaccg tgaccaaggc agccaacaaa ctgggcgtca aggttattac
    26341 ggtctatgct ttttctacgg aaaactggac ccgtccagat caggaagtca agtttatcat
    26401 gaacttgcca gtagagtttt atgataatta tgtcccggaa ctacatgcga ataatgttaa
    26461 gattcaaatg attggggaga cagaccgcct gcctaagcaa acctttgaag ctttaaccaa
    26521 ggctgaggaa ttgactaaga acaacacagg attgattctt aattttgctc ttaactatgg
    26581 tggacgtgct gagattacac aggcgcttaa gttgatttcc caggatgttt tagatgccaa
    26641 aatcaaccca ggtgacatca cagaggaatt gattggtaac tatctcttta ctcagcattt
    26701 gcctaaggac ttacgagacc cagacttgat tatccgtact agtggagaat tacgtttgag
    26761 caattttctt ccatggcagg gagcctatag tgagctttat tttacggaca ccttatggcc
    26821 tgattttgac gaagcggcct tgcaggaagc tattcttgcc tataatcgtc gtcatcgccg
    26881 atttggagga gtttaggagg aaatatgacc caggatttac agaaaagaac cttgtttgca
    26941 gggattgccc tggctatttt cctaccaatt ttaatgattg ggggcctctt gcttcagata
    27001 gcaatcggaa tcatagccat gctagccatg catgaacttt tgaagatgag aggtctagag
    27061 accatgacga tggagggcct cttgaccctc tttgcaacct ttgcattgac cattcccttg
    27121 gagaattacc tgactttttt gccagttgat gggaatgtgg ttgcctatag tgttttgatt
    27181 tcaatcatgt taggaacgac cgtttttagc aagtcttata cgattgagga tgcggttttc
    27241 cctcttgcta tgagcttcta cgtgggcttt ggatttaatg ctttactaga tgctcgtgtt
    27301 gcaggtttgg acaaggctct cttagccttg tgtatcgtct gggcgacaga cagtggtgcc
    27361 tatcttgttg ggatgaacta tgggaaacga aagttagcac caagggtatc gcctaataaa
    27421 acccttgagg gtgccttggg tggtatttta ggagcaattt tagtaaccat tatctttatg
    27481 atagttgaca gtacagttgc tcttccatat ggaatttaca agatgtcagt ctttgctatt
    27541 ttctttagca ttgctggaca atttggtgat ttactagaaa gttcgatcaa acgtcatttt
    27601 ggtgttaagg attctgggaa atttatccct ggacatggtg gtgttttgga tcgtttcgat
    27661 agtatgttgc ttgtatttcc aatcatgcac ttatttggac tcttttaatc aaaagacgga
    27721 ggaaacgcta tgctcggaat tttaaccttt attctggttt tcgggattat tgtagtggtg
    27781 cacgagttcg ggcacttcta ctttgccaag aaatcaggga ttttagtacg tgaatttgcc
    27841 atcggtatgg gacctaaaat ttttgctcac attggcaagg atggaacggc ctataccatt
    27901 cgaatcttgc ctctgggtgg ctatgtccgc atggccggtt ggggtgatga tacaactgaa
    27961 atcaagacag gaacgcctgt tagtttgaca cttgctgatg atggtaaggt taaacgcatc
    28021 aatctctcag gtaaaaaatt ggatcaaaca gccctcccta tgcaggtgac ccagtttgat
    28081 tttgaagaca agctctttat caaaggattg gttctggaag aagaaaaaac atttgcagtg
    28141 gatcacgatg caacggttgt ggaagcagat ggtactgagg ttcggattgc acctttagat
    28201 gttcaatatc aaaatgcgac tatctggggc aaactgatta ccaattttgc aggtcctatg
    28261 aacaatttta tcttaggtgt cgttgttttt tgggttttaa tctttatgca gggtggtgtc
    28321 agagatgttg ataccaatca gttccatatt atgccccaag gtgccttggc caaggtagga
    28381 gtaccagaaa cggcacaaat taccaagatt ggctcacatg aggttagcaa ctgggaaagc
    28441 ttgatccaag ctgtggaaac agaaaccaaa gataagacgg caccgacttt ggatgtgact
    28501 atttctgaaa aggggagtga caaacaagtc actgttacac ccgaagatag tcaaggtcgt
    28561 taccttctag gtgttcaacc gggggttaag tcagattttc tatccatgtt tgtaggtggt
    28621 tttacaactg ctgctgactc agctctccga attctctcag ctctgaaaaa tctgattttc
    28681 caaccggatt tgaacaagtt gggtggacct gttgctatct ttaaggcaag tagtgatgct
    28741 gctaaaaatg gaattgaaaa tatcttgtac ttcttggcaa tgatttccat caatattggg
    28801 atttttaatc ttattccgat tccagccttg gatggtggta agattgtgct caatatccta
    28861 gaagccatcc gccgcaaacc attgaaacaa gaaattgaaa cctatgtcac cttggccgga
    28921 gtggtcatca tggttgtctt gatgattgct gtgacttgga atgacattat gcgactcttt
    28981 tttagataat cgaggaatat tatgaaacaa agtaaaatgc ctatcccaac gcttcgcgaa
    29041 atgccaagcg atgctcaagt tatcagccat gctcttatgt tgcgtgctgg ttatgttcgc
    29101 caagtttcag caggtgttta ttcttatcta ccacttgcca atcgtgtgat tgaaaaagct
    29161 aaaaacatca tgcgccaaga attcgaaaag attggtgctg ttgagatgtt ggctccagcc
    29221 cttcttagtg cagaattgtg gcgtgaatca ggtcgttacg aaacctatgg tgaagacctt
    29281 tacaaactaa aaaaccgtga aaaatcagac tttatcttag gtccaactca cgaagaaacc
    29341 tttacagcta ttgtccgtga ttctgttaaa tcttacaagc aattgccact caacctttat
    29401 caaattcagc ccaagtatcg tgatgaaaaa cgcccacgta atggacttct tcgtacacgt
    29461 gagtttatca tgaaggatgc ttatagtttc cacgctaact atgatagttt ggatagtgtt
    29521 tatgatgagt acaaagcagc ctatgagcgt attttcactc gtagtggttt agacttcaag
    29581 gctattattg gtgacggtgg agccatgggt ggtaaggata gccaagaatt tatggccatt
    29641 acatctgctc gtacagacct tgaccgctgg gttgtcttgg acaagtcagt tgcctcattt
    29701 gacgaaattc ctgcagaagt gcaagaagaa atcaaggcag aattgctcaa atggatagtc
    29761 tctggtgaag ataccattgc ttactcaagt gagtctagct atgcagctaa cttagaaatg
    29821 gcaacaaacg agtacaaacc aagcaaccgt gttgtcgctg aagaagaagt tactcgtgtt
    29881 gcaacgccag atgttaaatc aattgatgaa gttgcagctt tcctcaatgt tccagaagaa
    29941 caaacgatta aaaccctctt ctacattgca gatggtgagc ttgttgcagc ccttctagtt
    30001 ggaaatgacc aactcaacga agtcaagttg aaaaaccact taggagcaga tttctttgac
    30061 gttgctagcg aagaagaagt ggcgaatgtt gttcaagcag gatttggttc acttggacca
    30121 gttggtttgc cagagaatat taaaattatt gcagatcgta aggtgcaaga tgttcgcaat
    30181 gcagttgtcg gtgctaacga agatggctac cacttgactg gtgtgaaccc aggccgtgat
    30241 tttactgcag aatatgtgga tatccgtgaa gttcgtgagg gtgaaatttc cccagatgga
    30301 caaggtgtcc ttaactttgc gcgtggtatt gagatcggtc atattttcaa actcggaact
    30361 cgctactcag caagcatggg agcagatgtc ttggatgaaa atggtcgtgc tgtgccaatc
    30421 atcatgggat gttacggtat cggtgtcagc cgtcttcttt cagcagtgat ggagcaacac
    30481 gctcgcctct ttgttaacaa aacgccaaaa ggtgaatacc gttacgcttg gggaatcaat
    30541 ttccctaaag aattggcacc atttgatgtg catttgatta ctgtcaatgt caaggatgaa
    30601 gaagcgcaag ccttgacaga aaaacttgaa gcaagcttga tgggagctgg ttatgaagtc
    30661 ttgacagatg accgtaacga acgtgtcggt gttaaattta gcgatagtga cttgattggt
    30721 cttccaatcc gtatcactgt tggtaaaaaa gcggccgatg gcattgtaga agttaagatc
    30781 aaggcaactg gtgacaccat tgaggttcat gcagataacg tgcttgaaac gcttgaaatc
    30841 ctaagcaaga aataaaaact ataatcagaa gaaaaacaag gaaaaaatgt aactagtttt
    30901 taccttgttt tttctgtata atgggagaaa tgagtagata aagaggtaaa tgttatgcta
    30961 agatttccaa aggattttgt ctggggatcc tctacttctg gaccgcagac agaaggacgt
    31021 gtagctggtg acggtaaggg agacaatctc tgggattact ggttccaagt ggagccaaat
    31081 cgttactata atgggattgg ttcagataag acatcgactt tttatgaaaa ttgggagcgg
    31141 gatattgagc ttttgttaga gactggtcac acagtctttc ggacttctat tcagtggtca
    31201 cggatttttc cacaaggctg tggaaaagtc aaccctcaag gtgtggattt ttatcgtaag
    31261 gtctttgagg ctattaaggc taaaggaatt cgtctgttag tcaatctcta tcattttgat
    31321 ttaccttttg cccttcaaga ggatggtgat ggttgggaaa ataaggcgac agtctcagcc
    31381 tatgaagact atgctcgttt ttgttttgag acttatggag atttagtgga tcaatggatt
    31441 acctttaacg agcccatcgt tcctgtagaa tttggttatt tttatgatgc ccattatcca
    31501 cataaggtgg atgcagaggc agctgttaag gtagcctatc atacacaatt ggccagcagc
    31561 cgggctgtta aggcttgcca tgaacttttg cctgattcca agattgggat tgtcctcaac
    31621 ttgacaccgg cttatccacg tagccagcat cctgctgatg tcaaggcagc tcgtattgcg
    31681 gccctttttc aggcccaatc tttcttagat ccatctgtct tggggactta tccacaggag
    31741 ttggtagaaa tcttgcatga acacggtctt ttacctgatg ctacagagga agagttggaa
    31801 ctcattcatg ataatacggt ggacttcctt ggtgtgaact actatcaacc tttgcgtgtt
    31861 atggctcctc gatttgctaa gcatccagag agtccactct taccagaaca tttttacgag
    31921 ccttatgtga tgcctggacg taaaatcaat tctcaccgtg gttgggagat ttatgagcaa
    31981 gggatttatg acatcgccca aaatatcaag gaaaattatg gcaatattga atggatgttg
    32041 actgagaatg gtatgggtgt tgaaggggaa gaaaaattcc gtcaagatgg aatgattcaa
    32101 gatgattacc gtattgactt tgtaaaaggt catcttcgtg aacttcaccg tgccattgaa
    32161 gatggtgcca actgtaaggg ctacttgatt tggaccttta ttgactgctg gtcatggctc
    32221 aacagctata aaaatcgcta tggtttggtc gaattagact tggaaacgca agaacgtcgt
    32281 ctgaagaaat caggccactg gttcaaaaaa ttaagcgata ataatggatt ttaaaaagga
    32341 ctctgactga ttatcccaat cggtcagagt tttttgctta caggagctta atttgaacta
    32401 taccaatttt tactcttgac aagtgaaaga aacaagtata tactgttttt gctgattcta
    32461 taaaagatga attagactat accaatttta aggagaaagc acagcttgcc tgtgtcgtat
    32521 atactatgtg tggaattgtt ggtgttgttg gaaacacaaa tgcaactgat attttgattc
    32581 aagggcttga aaagcttgaa taccgtggct atgattctgc gggaattttt gtcctagatg
    32641 gtgctgataa ccatttggtg aaggctgttg gtcgtatcgc agaattgtct gccaagacag
    32701 ctggtgttga gggaacaact ggtatcggac atactcgttg ggcaactcat gggaaaccaa
    32761 cggaagacaa tgctcaccca caccgctctg agacagaacg ttttgtcttg gttcacaatg
    32821 gggtgattga aaactacctt gaaattaaag aagaatacct tgctgggcac cacttcaaag
    32881 ggcaaacaga tacggaaatc gccgtacatt tgattggaaa atttgcggaa gaagacggtc
    32941 tctcagttct tgaagccttt aaaaaagctc ttcatattat ccgtggttca tatgcctttg
    33001 ccttgattga ctctgaaaat ccagatgtca tctatgtagc gaaaaacaaa tctccacttt
    33061 tgattggtct tggggaaggc tacaatatgg tctgctcaga tgctatggct atgattcgtg
    33121 aaaccaacca atacatggaa attcatgacc aagagttggt aatcgtcaag gctgatagcg
    33181 tggaagttca agactatgat ggaaatcgtc gtgagcgtgc tagctacact gctgaacttg
    33241 acttgtcaga tatcggtaag ggaacttacc cttactacat gctcaaggaa attgacgagc
    33301 aaccaactgt tatgcggaaa ctcatccaag cctacacaga tgaggctggt caagttgtcg
    33361 tagacccaga tatcattaag gctgttcaag acgcagaccg catctacatc cttgcagctg
    33421 gaacatctta ccatgcagga tttgcttcta agaagatgtt ggaagaattg acagatacac
    33481 cagttgaact tggaatctca tctgagtggg gctacggtat gccacttctc agcaagaaac
    33541 cactcttcat ctttatcagc caatctggtg aaacagcgga tagtcgtcaa gttttggtca
    33601 aggctaatga aatgggaatt ccaagcttaa cagtgacaaa tgttccaggt tcaaccctct
    33661 cacgtgaagc caactatacc atgctccttc acgcaggtcc tgaaattgcc gtggcatcaa
    33721 ctaaagccta tacagcgcaa atcgcagccc ttgccttcct tgcaaaagca gtcggagaag
    33781 caaatggtaa tgctaaagcg caagcctttg acctggttca tgaattgtca atcgtagctc
    33841 agtctattga atcaactctt tcagagaaag aaaccattga agccaaggtt cgtgaacttc
    33901 ttgaaacaac tcgtaacgcc ttttacatcg gacgtggtca agattattac gtagccatgg
    33961 aagcaagtct caaactcaaa gagatttctt atatccagtg tgaaggtttt gcggcaggag
    34021 aactcaagca cggaaccatt gccttgattg aagaaggaac gcctgttttg gctctcttgt
    34081 cagatccagt tcttgccaac catactcgtg gaaatatcca agaggtcgca gcccgtggtg
    34141 ccaaagtcct cactatcgca gaagagaatg tagccaaaga taccgacgat atcgtcctta
    34201 cgaccgtaca tccatacctc tcaccaattt caatggtcgt accaacgcaa ttagtcgctt
    34261 actttgcaac cctccaccgt ggcctcgatg tggacaaacc acgtaacctt gccaagtcag
    34321 taacggtaga ataagctaaa aaagtctagt tatctaggct ttttcttgtg agcaaattgg
    34381 ttgcttgtac tcaagattcg tgttatactc ttagaaaatc tcttcaaacc acgtcaccgt
    34441 cgccttgccg taggtatggt tactga
//
LOCUS       DAVBCV010000017        34395 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBCV010000017 DAVBCV010000000
VERSION     DAVBCV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.017"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            1..4834
                     /locus_tag="VQ516_000809"
                     /pseudo
     CDS             1..4834
                     /locus_tag="VQ516_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(5071..5619)
                     /locus_tag="VQ516_000810"
     CDS             complement(5071..5619)
                     /locus_tag="VQ516_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2511280.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            5940..6428
                     /locus_tag="VQ516_000811"
     CDS             5940..6428
                     /locus_tag="VQ516_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2511281.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            6425..7612
                     /locus_tag="VQ516_000812"
     CDS             6425..7612
                     /locus_tag="VQ516_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2511282.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            7605..7970
                     /locus_tag="VQ516_000813"
     CDS             7605..7970
                     /locus_tag="VQ516_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2511283.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            7986..9332
                     /gene="asnS"
                     /locus_tag="VQ516_000814"
     CDS             7986..9332
                     /gene="asnS"
                     /locus_tag="VQ516_000814"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2511284.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            9485..9775
                     /locus_tag="VQ516_000815"
     CDS             9485..9775
                     /locus_tag="VQ516_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2511285.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            9787..10257
                     /gene="ssbA"
                     /locus_tag="VQ516_000816"
     CDS             9787..10257
                     /gene="ssbA"
                     /locus_tag="VQ516_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2511286.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            10289..10528
                     /locus_tag="VQ516_000817"
     CDS             10289..10528
                     /locus_tag="VQ516_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2511287.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            10823..12223
                     /locus_tag="VQ516_000818"
     CDS             10823..12223
                     /locus_tag="VQ516_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2511288.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            12225..12584
                     /locus_tag="VQ516_000819"
     CDS             12225..12584
                     /locus_tag="VQ516_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2511289.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            12638..13387
                     /locus_tag="VQ516_000820"
     CDS             12638..13387
                     /locus_tag="VQ516_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2511290.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            13377..13676
                     /locus_tag="VQ516_000821"
     CDS             13377..13676
                     /locus_tag="VQ516_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2511291.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            13747..14682
                     /locus_tag="VQ516_000822"
     CDS             13747..14682
                     /locus_tag="VQ516_000822"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2511292.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            14769..15281
                     /locus_tag="VQ516_000823"
     CDS             14769..15281
                     /locus_tag="VQ516_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2511293.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGAVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            15318..15913
                     /locus_tag="VQ516_000824"
                     /pseudo
     CDS             15318..15913
                     /locus_tag="VQ516_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            16014..16208
                     /locus_tag="VQ516_000825"
     CDS             16014..16208
                     /locus_tag="VQ516_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2511294.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(16254..17699)
                     /locus_tag="VQ516_000826"
     CDS             complement(16254..17699)
                     /locus_tag="VQ516_000826"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2511295.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            17805..19427
                     /locus_tag="VQ516_000827"
     CDS             17805..19427
                     /locus_tag="VQ516_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2511296.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            19631..21589
                     /locus_tag="VQ516_000828"
     CDS             19631..21589
                     /locus_tag="VQ516_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2511297.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFATAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            21761..22696
                     /locus_tag="VQ516_000829"
     CDS             21761..22696
                     /locus_tag="VQ516_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511298.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFE"
     gene            22721..23373
                     /locus_tag="VQ516_000830"
                     /pseudo
     CDS             22721..23373
                     /locus_tag="VQ516_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            23531..24625
                     /locus_tag="VQ516_000831"
     CDS             23531..24625
                     /locus_tag="VQ516_000831"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2511299.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            24633..25799
                     /locus_tag="VQ516_000832"
     CDS             24633..25799
                     /locus_tag="VQ516_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2511300.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            25892..28990
                     /locus_tag="VQ516_000833"
     CDS             25892..28990
                     /locus_tag="VQ516_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2511301.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            29038..29655
                     /locus_tag="VQ516_000834"
     CDS             29038..29655
                     /locus_tag="VQ516_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2511302.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            29665..30999
                     /locus_tag="VQ516_000835"
     CDS             29665..30999
                     /locus_tag="VQ516_000835"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2511303.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            31009..31473
                     /locus_tag="VQ516_000836"
     CDS             31009..31473
                     /locus_tag="VQ516_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511304.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            31470..31964
                     /locus_tag="VQ516_000837"
     CDS             31470..31964
                     /locus_tag="VQ516_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511305.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            32045..33694
                     /gene="mltG"
                     /locus_tag="VQ516_000838"
     CDS             32045..33694
                     /gene="mltG"
                     /locus_tag="VQ516_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2511306.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMTDSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDIIS
                     DTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPIDA
                     NSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQKSM
                     STEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAFLA
                     KVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDEML
                     AAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDMPL
                     QSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIESSI
                     NQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            33765..34247
                     /gene="greA"
                     /locus_tag="VQ516_000839"
     CDS             33765..34247
                     /gene="greA"
                     /locus_tag="VQ516_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2511307.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT        10730 a         6381 c         7794 g         9490 t
ORIGIN      
        1 gcggtctaac tggagctcaa ggagctaaag gtgaaaaagg agcccaaggt gaacgcggtt
       61 taactggagc taaaggtgaa aaaggggacc aaggtgaacg cggtctcact ggagcccaag
      121 gtgaaaaagg agaccgaggc gaacgtggtt taactggagc taaaggtgaa aaaggagccc
      181 aaggtcaggc aggtcgggtc gtgacggtgt aactccaacc gtaaccgtta aagataataa
      241 aaatgacggc actcatacta tcactattaa cgacggtaga ggtaatgtta caagtactgt
      301 tgtaagagat ggtttcgatg gcgcaagtcc attagttgcg actcaacgaa atgacgcaga
      361 taagacaaca actgttatct tctattacga taaaaatgga aacaatgaat tagatgcttc
      421 tgataagaaa ttaaaagaag ttgtaattgc agatggtgct aaaggtgaaa aaggggacaa
      481 aggtgaacaa ggtcttcaag ggcgtgatgg tgaacaagga ccaaaaggag aagatgggaa
      541 aaccccaaca gttaaagtaa ctgatggtca agatggaacg catacaatta caattaacga
      601 tggtaaaggt ggtataacta ctacagtagt aagagatgga tttgatggtg caagccctct
      661 tgtttctact catagaaatg aagcagataa aacaacaact gttattttct attatgatct
      721 aaatgataat aatcaatttg atgaaggaga tacaaaactt aaagaagttg ttatcgcaga
      781 tggaaaacaa ggaccaaaag gtgacaaagg tgataacgga aaagatggat tcacaccaga
      841 agtaacagtt acagataaca ataacggaac acacaccatc acaatcacac aaccagacaa
      901 cagaccatca ttaacaacaa tcgttaaaaa cggtgaagat ggaaaaacac caaaagtcaa
      961 agcagaacga gatgatgcga agaaacaaac aacattaaca ttctacattg acaaagatgg
     1021 agatggaagt tacacagcag gaaaagacga gttagttcaa acaacagtag ttaaagacgg
     1081 acaagacgga gctgcaggag catctggacg tgatggaaaa gaagtattaa acggaaaagt
     1141 agacccaaca acagaaggta aagatggaga cacattcgta aatacacaaa caggagatgt
     1201 attcgttaag aaaggtaaca cttgggaacc agcaggaaac atcaaaggac cgaaaggtga
     1261 caaaggtgca gatggtgcca agggtgaaaa aggagcccaa ggagaacgcg gcctgactgg
     1321 agcgcaaggt gtcaagggtg aaaagggaga tcagggtgaa cgcggtctaa ctggatctaa
     1381 aggtgaaaaa ggagaccaag gtgaacgcgg tttaactgga gcgcaaggtg ccaaaggtga
     1441 caaaggtgaa caaggtcttc aaggtcgtga tggagctcaa ggaccaaaag gcgcagatgg
     1501 acaaagagga ccagcgggac cacaaggacc aaaaggagaa caaggtaatc caggaactcc
     1561 aggtaaagat ggaaaatctc taattgctgt taaaaatgga actgaaacaa aagtctatgt
     1621 agaggatccg gctagaccag gacaaccact taatccaaac caaccattag caactatcac
     1681 tgatggaaga aacggtacta atggacaatc accaacaatt acagcgacac gaagtgtgca
     1741 aaatggcaaa aatggtgtat tagtaacaat tacaccagta ggaggtcgtc cacaaacaac
     1801 atttgtagag gatggacaaa agggtgctga tgggaaaacc ccaacagtaa caataactga
     1861 ggggcaaaac ggcacacata cattaacggt tcataatcca ggaagtccag atgtgacaac
     1921 tacgatccgt gatggagcta caggacaagc aggtcgtgat ggtaaagatg tattaaacgg
     1981 aaaagtaaat ccacaaccaa accaaggtaa aaatggagat aaatatatta ataccgaaac
     2041 cggtgatgtc tatgttaaaa acaatggaaa ctgggataaa gaaggcaaca tcaaaggccc
     2101 taaaggggac aaaggtgcag atggtgctaa aggcgaaaaa ggagaccaag gcgaacgcgg
     2161 cctaactgga gcgcaaggag ctaaaggtgc ggatggcgca gtaggtcgtg atggacgtga
     2221 cggtaaagac gtgttgaacg gcaaagctaa cccagaagca catcaaggta aagacggcga
     2281 taaatacgtt aatacagaaa caggcgacgt cttcgttaag aataacggca actgggataa
     2341 agagggcaac atcaaaggcc ctaagggtga caaaggtgaa cgcggagaag atggtaagac
     2401 tccagaagta actgtaactc caggtaaaga tggccatagt actgacatta cattcactgt
     2461 tccaggtaaa gatccagtta cagtaaatgt taaggacgga gaaaatggtc tgaacggtaa
     2521 aactccaaaa gttgatttac ttcgtgtcca aggaaaaaac ggaaatccat ctcatacaat
     2581 tgtgacattc tatacagatg aaaacaatga cggcaaatat acaccaggaa ctgatgaact
     2641 tctaggttca gaaatgatta aagatggtgc taaaggcgcg gacggacgag atggtaaatc
     2701 attgcttact gtcaaggatg gtaaagaaac taaagtttac caagaagatc cagctaaccc
     2761 aggacaacca ttaaatccag aaaaaccact tgcggtaatt agagatggag tagatggaaa
     2821 atcacctaca gttacagctg ttcgtaaaga tgaagcaggg cataaaggtg tagaaatcac
     2881 tgttgataac catgatggtt cacaaccaac tacagtcttt gttcaagatg gtgctaaggg
     2941 agaaactggt gcaaccggtc aggatggaca aactcctaca atcactactc aacgtggaca
     3001 agatggccaa agcactgttg taactatcac aacatcaggt aaagatccag taaccttcac
     3061 tgtaaaagat ggtaagaatg gtaaagatgg ccgtgcaccg aaaatcaaag tagaagatat
     3121 tacttcacct tcaagaatta gacgcgatac agatgctgct gcaactccaa cgcgtaacgg
     3181 tatccgtgtt acagtttatg atgatgttaa tgacaatggg gtatacgacg aaggtgtcga
     3241 taaagtatta aatagtaaag atatttataa cggtatagat ggacgtgatg gttcagctcc
     3301 aactattact acaaaagata atggagatgg aactcacact atcacagttc aaaacccaga
     3361 tggttctgaa tcaacaacag ttgttaaaga tggtaaagac ggtaaaactg cgaatatcac
     3421 tacaacagaa aacccagatg gaagccacac aattacggtg acaaatccag atggttcaac
     3481 taaagaaact gttgttaaaa acggtaaaga cggtaagact cctaaagttg aagtaacgga
     3541 taacaacgat ggaactcata ctgttaaagt tacagatgga gacggcaatg ttaccaacgc
     3601 tatcatcaaa gatggtaaag acggtaaagc tgcaacagca acaactactg aaaatccaga
     3661 tggaagccac acagtaacaa tcactaaccc agacggaact aagaatgagt ttgttgttaa
     3721 gaatggacgt gacggtgttg acggacgtac tccaaccgca tctgttcgtg ataatggaga
     3781 cggaagtcat acaatcgtta ttacaaatcc agaaggtgtg acaactgaaa ccacagttcg
     3841 tgatggtaaa tcaccaaaag tgactataac tgatgaacaa aatggaactc ataagatctc
     3901 tgttctaaat ggtgacggaa caactacaac aactacagtt cgtgatggta gagaaccaaa
     3961 acttgaagtt attgataaca acgatggttc acacactatt aaagtgacag gtgctgatgg
     4021 taaaggaacg acaactacaa tctttgatgg taaatcacca aaagcgaaca tcgttgataa
     4081 cggagatgga actcatacat taacaatcgt agattctgat ggtcgtgaat acaaatctat
     4141 tatcaaagat ggtaaagacg gcaaagatag cgtttcacca actgtaactg ttaaaaataa
     4201 taacgatgga actcacgttg ttacaatcac taatccagat ggaagtaaga cagaaatggt
     4261 gattaaagac ggtaaagatg gtaaatcacc aaaagtttct gttgaagata atggtgatgg
     4321 tagtcataca atcacaatca tcaattctga tggaactgtg acaaaaacag ttattaaaga
     4381 tggtagagat ggacgtgatg gtcgagacgg caaagacggt aaagacggta aagatggaaa
     4441 atgtggatgc caagacaaac cagtaacacc atcaaatgac aaaccagttc ctccaacacc
     4501 aaatgtgccg acaccagaag taccggttaa acctgtgcca gcgcaaccaa caccaaatgt
     4561 accgacacca gaagtgccag tacaaccaac tccagctgtt ccaacaccag aagtaccggt
     4621 taaaccagta ccagcggttc cagaacaacc agtagtacca acaccggctc aaccagcaac
     4681 tccagtaaat gctaacccag tagcaccaac tacaggtaaa gaaaaccgtg gggacaaatt
     4741 acctgaaact ggaagccaat ctgattatat ctctgttctt ttaggtagcg gtattctatt
     4801 gagcctatat gtaggacgaa gaaaagaagc ttaatcagcc aaaagcagtc actaatttct
     4861 cgaaagaaga tgataagtga tagatctcga atgaaagaat attcttagaa tgattttcct
     4921 atgatggaaa agactgctaa aaaccttgag tggtaacgct caaggttttt ttggatggct
     4981 gatgtttgtc tgaaaatctt gattgagaaa taaaaagcca ccctattagg tggctataat
     5041 tgatttagac tttctaaaat tttctatttt ttactgaatc gcttcgacca agtcttgagc
     5101 ttgtttttca agtgagttta ggactgtttc ttcaagaacc aattttccgt ctgcccaggc
     5161 agagtcatta acacgtgcag cagtgaaatc accaacgcct tgtgtacgga taaatggcaa
     5221 gaggtctttg tagatagcga aaagttgatc gtgccctgca ttggctacag atgatacggt
     5281 gacaaacttg tcttgaaggg cagaaacgcc acgcgtatca gacaagtcaa gggcacgaga
     5341 tagccagtca agcaagtttt ttactgtacc agggatagag aagttgtaga ctggagagaa
     5401 aatccagata gcatccgcaa cgagaactgc ttcacgagca gcagctacag ctggatgtgt
     5461 tggaacttcc aaatcttggc tgaagagagg aagggctgaa taatcaaggt agctaacttc
     5521 cgctttacca gcaagtgctt tctcagcttc gagcgccatt tggtggttga aagaaccttg
     5581 acgtagtgat ccgacgataa ataatacttt tttagacatg atgtgtctcc tttttcttca
     5641 aattttgaga actctctcgt tgttataaaa tacattacaa caaaacaaaa gaacttgcaa
     5701 gaaatttgct cagagtttat ttgaaaattg taaaatttcg gaaactctag tcatttgtga
     5761 ctgaaaaagt aagaactgtt tcactttcaa aatcggtttt gtttgaaggg agaaatagaa
     5821 ttggttgctc aaagcacagc tttgaggttg cagataaagc tgacgtggtt tgaagagatt
     5881 ttcgaagagt attcttatat tcacttgtaa taaagcttaa tagatggtaa aatagacgag
     5941 tgaaaaaaag acaaaacaaa gcaaaaaata atctactgtg gcagtatggt ctagggatga
     6001 cgattttgtt tgtggttatc agtgcttcct ttctgtatat ggtttctctt agcatgaaac
     6061 cctatcaaac agctaaaagt gaaggagaaa aattagctca gcagtatgca ggattagagc
     6121 aggccgatca ggttgattta tacaatggct tggaatctta ttacagcgtt cttggtcgta
     6181 ataaacagca agaagcactt gctgttctga ttggaaaaga tgatcataag atttacgttt
     6241 atcagctaaa tcagggtgtt tcacaagaaa aagcagaaac ggtttctaag gaaaagggag
     6301 ctggcgaaat tgacaagatt atctttggtc gttatcaaga taagccaatc tgggaagtca
     6361 agtcaggatc tgatttttat ctagtagatt ttgaaacagg agcattggtc aacaaggagg
     6421 gcctatgaaa ttatccaacc gtgttttaga aatggaagaa agtgtgactt tggctgctgg
     6481 agcccgtgcc aaagcactga aggctgaggg tagagatatt ttgtctctaa ccttgggtga
     6541 gccagatttc actactccca aaaatatcca agatgccgcc attgcatcga ttcgagatgg
     6601 gcgtgcttct ttttatacag taacctcagg tctgccagag cttaaggcgg cggtcaatag
     6661 ctactttgag cgcttttacg gctattctgt agcgtcaaat caagtgacag tcgctgcggg
     6721 agccaaatat tctctctata ccttctttat ggctgtggtc aatccaggtg atgaagtcat
     6781 catcccaacc ccatactggg ttagctatgg agatcaggtc aagatggcag aaggcgttcc
     6841 cgtctttgtt tctgctaagg aagacaatca ctttaaggtg accgtagagc agttagaagc
     6901 agctcgcact gacaagacca aggttttggt gctgaattcg ccatctaatc ccacaggtat
     6961 gatttacacc cgtgaggaac tcttggcaat tggaaactgg gctgtagaaa atgatattct
     7021 catcctagca gatgatatct atggccgctt ggtttataat ggtcatgagt tcacaccgat
     7081 ttctagccta tcggaagcga ttcgcaagca aacagtggtc atcaatggtg tgtctaaaac
     7141 ctatgccatg actggttggc ggattggtta tgccgttgga gaagcagaca ttatcgctgc
     7201 catgtccaag attgcaggtc aaacaacttc gaatccgtca gcagtagccc aatatgcagc
     7261 agttgaggct ctatcaggtg agcaagatac tgtagaaagc atgcgtcagg cctttgaaga
     7321 acgtcttaat accatctatc ccctccttgc agaagtgcca ggatttgaag tggtcaaacc
     7381 gcaaggggcc ttctatctct tcccaaatgt caaaaaggcc atggagatga aaggctacac
     7441 ggatgtgaca gactttacaa ctgttatctt agaagaagcc gaagtggcct tggtaacagg
     7501 agctggattt ggagcaccag aaaatgtccg cctcagctat gcgacagacc tagacacgct
     7561 taaagaagca gtcgaacgct tgaaagcatt tatgggtagt gagaatgatt gatcattttg
     7621 agattaaggt aaaggattta caaatttcag aaggatttta taggagtttt ctcgctcctt
     7681 tggactataa attggctttt aagactagtt ctctaattag ttttctatcc ccgaacagcc
     7741 ctcatcctgg tggtgatttt tggctgactc agggaacaca agatcctgtt cattttgctt
     7801 ttttagcaga aaataaggaa gaagttcagg cttgttatga ggctggctta gaggcaggtg
     7861 ggcgagacaa tggggctcct ggttatcgaa gtgagcatcc gatttactat gctgctttta
     7921 tgattgacct ggatgggaac aatatagagg tggtttgcca taaagaataa aaaagaaaag
     7981 gaaaaataat gacaaaacgt gtaacgatta ttgacgtaaa agactatgtt ggtcaggaag
     8041 tgacgattgg cgcttgggtt gccaacaaat caggaaaagg gaaaatcgct ttcttacaat
     8101 tgcgtgatgg aacagccttc tttcaaggtg tggcttttaa accaaacttt gtcgaaaaat
     8161 ttggtgaaga agtgggactt gagaagtttg atgttatcaa acgcttgagc caagaaacgt
     8221 ctgtttatgt gacaggtatt gtcaaagagg acgaacgttc taaatttggc tatgagttgg
     8281 acatcacaga catcgaagtg atcggtgaat ctcaagacta cccaatcaca ccaaaagaac
     8341 acggaacaga ctttttgatg gataaccgtc acttgtggct acgctctcgt aagcaagtag
     8401 ctgtgttgca aatccgtaac gctattatct atgcaactta tgagttcttt gacaagaacg
     8461 gttttatgaa gtttgacagc ccaattcttt caggaaatgc ggcagaagat tctacagaac
     8521 tctttgaaac tgactacttc ggaacgccag cctacttgag ccaatcaggt caactttacc
     8581 tagaagcagg ggctatggct cttggtcgtg tctttgactt tggtccagtt ttccgtgctg
     8641 aaaaatcaaa aacacgccgt cacttgactg agttctggat gatggatgct gagtactcat
     8701 acttgacaca tgatgagtcg cttgacttgc aagaagctta tgtgaaagct cttctacaag
     8761 gtgttcttga ccgcgcgcct caagccttgg aaaccttgga acgtgataca gaactcttga
     8821 aacgctacat tgcagagcca ttcaaacgta tcacttacga tcaagccatt gacctcttgc
     8881 aagagcatga aaatgatgaa gatgctgact acgagcatct tgagcatggt gatgactttg
     8941 ggtcaccaca cgaaacttgg atttcaaacc actttggtgt gccaacattt gtcatgaact
     9001 atccagcagc catcaaggcc ttctacatga aaccagttcc tggaaatcca gagcgcgtgc
     9061 tttgtgcaga cttgcttgct ccagaaggct atggagaaat tatcggtggg tctatgcgtg
     9121 aggaagatta cgatgccctt gtcgctaaga tggatgaact tggcatggat cgtacagaat
     9181 atgaattcta ccttgacctt cgtaaatacg gtacagttcc acacggagga tttggtatcg
     9241 gtatcgaacg tatggtaacc ttcgcagcag gaacaaaaca tatccgtgaa gctattccat
     9301 tcccacgtat gttgcaccgt atcaaaccat aagaacagga aaacgcccat gtggcgtttt
     9361 ttcgaattct agtgttttta cttgcgtaaa aaaaattttt ctagtataat agtttattgt
     9421 gagcgaacct cacttacccc ttgcaaagtc ttggggtcat tagaccaaaa ggaggaacat
     9481 atcaatggct aaatacgaaa ttctttatat cattcgtcca aacattgaag aagaagctaa
     9541 aaacgctttg gtagcacgtt ttgactctat tttgactgac aacggtgcaa ctgttgttga
     9601 atcaaaaact tgggaaaaac gtcgtcttgc atacgaaatc caagatttcc gtgaaggact
     9661 ttaccacatc gttaacgttg aagcaaatga cgatgcagct cttaaagagt ttgaccgtct
     9721 ttcaaaaatc aacgctgaca ttcttcgtca catgatcgtc aaaattgacg cgtaagaagg
     9781 taaactatga ttaacaatgt tgtacttgta gggcgtatga cacgtgacgc tgaattgcgt
     9841 tataccccat caaatgtagc agttgcgact tttactcttg cagtaaaccg tacatttaag
     9901 agtcaaaatg gtgaacgtga ggctgatttt atcaatgtcg ttatgtggcg ccaacaggct
     9961 gaaaatcttg ctaactgggc taaaaaaggc tcacttatcg gggtgacagg tcgtatccag
    10021 actcgtagtt acgataacca gcaaggacaa cgtgtctacg tgacagaggt cgtggctgag
    10081 aatttccaaa tgttggaaag ccgtagtgtg cgtgagggtc acacaggtgg agcttactct
    10141 gcaccaactg caaactattc agcacctaca aattcagtac cagacttttc acgtaatgaa
    10201 aatccatttg gagcaacaaa cccattggat atttcagatg atgatttacc attctaatgg
    10261 acaactaaac ttataaagga gaaaaaacat ggctcaacaa cgtcgtggcg gattcaaacg
    10321 ccgtaaaaaa gttgattaca tcgcagcaaa caaaattgaa tatgttgatt acaaagatac
    10381 tgagcttctt agccgtttcg tttcagaacg tgggaaaatc cttcctcgtc gtgtaacagg
    10441 aacttcagct aaaaaccaac gtaaagtaac aacagctatc aaacgcgctc gcgtaatggc
    10501 tttgatgcct ttcgtaaacg aagattaaag aaaagacccg gttgggtctc tttttcatga
    10561 acttgaaaac aagaaagcga attaagaccc caacgggtct tttttcacaa gctttgaaat
    10621 gagagagcga gttaggctca gtggctccct ttttacgagt ttgaaaacaa gagaacacat
    10681 taaaagcacg atggagcttt ttcataaata tgtctttttt ttcatccaag aaattaaaat
    10741 aaaaatgata aactttttga aaatagggga tttgcctact ttatgatata atggaaagag
    10801 ttagattgaa agaggtagtg agatggacgc gaaattaaga tacaaggcaa agaagatcaa
    10861 gattgtcttt tttgatattg atgatacatt gcggaattca aagacagggt ttattccaac
    10921 tacaattccc actgttttta aacagttgcg tgaaaaagga attttaacag gaatcgcctc
    10981 tggacgtggc atttttggtg ttgttccaga gattcgtgat ctcaaacctg acttttttgt
    11041 aactttgaat ggggcttata tcgaagataa aaaaggtcag gtcatttatc agcatcagat
    11101 tgaaaagtca tatgttgagg agtatatctc ttgggctaag caagaaggaa ttgagtatgg
    11161 cttggttggg agtcatgatg ccaagttgtc gactcgcacc gatatgatga gtgaagctat
    11221 caatccaatt tatcccgact tagatgtaga tcccgatttc catgaaaaag aagatatcta
    11281 tcagatgtgg acttttgaag ataagggaga tgacttgcac ttgcctgaca gtctctcaga
    11341 caaacttcgc atggttcgtt ggcatcaaca ttcgtctgat attgtgccga tttcaggctc
    11401 caaagcgacg ggggtggaaa aggttgtgga acaccttggc ttgaaaccag agaaggtcat
    11461 ggtttttgga gatggtctca atgacttgga actctttgat tatgctggaa tcagcgttgc
    11521 catgggaatt tctcatgata aaatcaaaga aaaagcagat tatattacaa aaacattaga
    11581 agaagatggc atttttgatg ccttagaagt atttggtatg gtagaaaaag aattgcattt
    11641 tccacaagta gacattgaaa cagtagaagg tcctcttgcg actattaaga ccaatcacgg
    11701 agacttacgt atcaagcttt tccctgaaca tgctcctaaa acagtggcta actttgtatc
    11761 tctttcaaaa gatggctact atgatggtgt cattttccac cgtattatca aggactttat
    11821 gatccaaggt ggagacccaa ctggaactgg tatgggtggc gagtcaatct acggcgaatc
    11881 ttttgaggat gaattctcag aagagcttta caatatccgt ggtgctcttt ccatggcaaa
    11941 tgctggtcca aataccaacg gcagccagtt ctttatcgtg caaaaccaac acctacctta
    12001 ttctaagaaa gaaattactc gtggtggttg gccagaaccg attgcagaaa tctatgccaa
    12061 tcaaggtggg acacctcacc tagaccgccg tcacacggtt tttggtcagt tagctgatga
    12121 agcatcttac gctgtcttgg atgtcattgc tgctgttgag acaggagcta tggacaagcc
    12181 agttgaagat gttgtaattg aaactattga aatcgaggac taagatgaaa atcggtgata
    12241 agctaaaggg gcgtattaca gggattcagc cctacggtgc ctttgttgag ttagagacgg
    12301 gtgatacggg gctgattcac atttcagaaa ttcggacagg atttattgaa aatattcatg
    12361 aaaccttgaa agtcgatgaa gaggttcaag ttcaggtagt ggatttagat gaatttacgg
    12421 ggaaagccag tctttctatc cgcactttgg aggaagaaaa gtaccagttt cctagacgga
    12481 gacgcttttc aagcgaccgc tttaactacg gttttgctcc ctttcgacga atgctaccaa
    12541 tctggactgg agaagccctg caccatttaa aaaagaagaa gtagtttaga gttttatatt
    12601 ttttgtaatg ttaatataaa tttgctataa taggaccatg gaagaagaac aattattaaa
    12661 atcaggagag cgcattaacc agctcttttc gacagatatc aaaatcattc aaaatagaga
    12721 ggtttttagc tattcggtgg atagtgttct cttatcacga tttccacgtt ttcctaagaa
    12781 ggggttgatt gtggatttct gtgctgggaa tggagcagtg gggctttttg ctagcactcg
    12841 tactcaagca cagatattgt ctgttgagat tcaggagcgt ttggcggata tggctgaacg
    12901 ctctgtccgt ttgaatggat tagaggagca aatgcaggtc atctgcgatg atttgaaaaa
    12961 tatgcctgct cacattcagg ggagtaaggt ggatatgatt ttgtgtaatc caccctattt
    13021 caaggtggat ccttattcta atctgaacga gagtgaacat tatctcttgg ctcgacacga
    13081 aatcacgact aatttggaag aaatctgtca tagtgcccag agtattctca agtctaatgg
    13141 gcgtttggcc atggttcatc gtcctgatcg acttctggat attttggata cgttaaaacg
    13201 acataatcta gcccctaagc gcctgcagtt tgtttatcca aaaagagaaa aggaagccaa
    13261 tatgcttttg attgaggcga tcaaggatgg ctcaacaagt ggctttaagg tcttacctcc
    13321 tttgattgtc cacaatgatg atggctctta tacgcccgaa atcgaagaga tttattatgg
    13381 atcataaggc ttatatgtac gttctggagt gtcgtgatgg atcctactat ataggctata
    13441 cgactgatat gagaagacgc ctcgctatcc acaatagtgg gaagggagcc aaatatacac
    13501 gagcacgctt gccagtcaaa cttatctatg ctcaaggttt tgccagtaag gaagaagcca
    13561 tgtcggctga agctcttttc aagcgtaaga agaggccaca gaaggaagaa tttttatctg
    13621 aaaatcaaga tagaaattta ctcagttatt ttgaagaatc gtggggagtc ctttgactcc
    13681 tcttaccttt gtcaaaaagc gaaaaaaatg ctacaataaa gaaaataaac aaaatgaggt
    13741 attatcatgt ccaagattct agtatttggt caccaaaatc cagactcaga tgccatcggg
    13801 tcatctgtag cttttgccta ccttgcaaaa gaagcttacg gtttggatac ggaagctgtt
    13861 gcccttggaa ctccaaatga agaaacagcc tttgtcttga actattttgg tgtggaagca
    13921 ccacgtgtta tcacttctgc caaagcagag ggggcagagc aagttatctt gactgaccac
    13981 aatgaattcc aacaatctgt atcagatatc gctgaagtag aagtttacgg tgttgtagac
    14041 caccaccgtg tggctaactt tgaaactgca agcccacttt acatgcgttt ggagccagtt
    14101 ggatcagcgt cttcaatcgt ttaccgtatg ttcaaagaac atggtgtagc tgtgcctaaa
    14161 gagattgcag gtttgatgct ttcaggtttg atttcagata cccttctttt gaaatcacca
    14221 acaacacacc caacagataa aatcattgct cctgaattgg ctgaattggc tggtgtaaac
    14281 ttggaagaat atggtttggc aatgttgaaa gctggtacca acttggctag caaatctgct
    14341 gaagaattga ttgacatcga tgctaagact tttgaactca acggaaataa tgtccgtgtt
    14401 gcccaagtga acacagttga catcgctgaa gttttggaac gccaagcaga aattgaagct
    14461 gcaatgcaag ctgccaacga atcaaacggc tactctgact ttgtcttgat gattacagat
    14521 atcgtcaact caaactcaga aatattggct cttggtgcca atatggacaa ggtcgaagcg
    14581 gctttcaatt tcaaacttga aaacaatcat gccttccttg ctggtgccgt ttcacgtaag
    14641 aaacaagtgg tacctcaatt aactgaaagc tttaatgcgt aagattttgg gtgtcagctc
    14701 aaaatcggaa agtctagttt gccttatatc gaaaggagtt tcggctcctt tttttctagg
    14761 agtgaagtat gttagaaaat ggcgatttga tttttgtgag agatgggtca gacatgggac
    14821 aggccatcca gacttccaca ggtaactata gccatgttgc catttatttg gatgggatga
    14881 tttatcatgc tagtggacag gctggtgctg tctgtcaaga accggcagac ttctttgagt
    14941 ccaatcattt gtacgacctc tatgtttacc cagaaatgga tatccagtcg gtgaaggaaa
    15001 gagcttgcag acatcttgga gcaccctaca atgcttcttt ctatccagat gcagctggtt
    15061 tctactgctc ccagtatata gcagaaatcc tacctatttt tgaaactatt cctatgaaat
    15121 ttggagatgg ggaacaggag attagtgatt tttggaggga gtattacata gaactaggtc
    15181 tgcctgttcc tctgaaccaa gctgggacca atcctagtca gttggcagca tcgcctctgt
    15241 tacaatgtaa agaaaggaat cttcatgatt cagattttta attcatctcg tttgacgaga
    15301 cagccatttt tggagaattg atccgctatc tggaccagta tgaggatgtg attctacggg
    15361 aaattaaggc tcaatttcca gatgttgcag ttgataaact catggaagag tatataaagg
    15421 caagcttgat tctacgtgaa aataagcgct attacctcaa ttttcctacg cttgaatcac
    15481 ttgatagtct tgaactggat caagagattt ttgtcagaga agctagtccg gtctatcaag
    15541 ccttgttgga gcagagtttt gagacggaat tgcgcaatca aatcaatgca gctattttag
    15601 ttgaaagacg gactttgcgc gcattaaaat gaccctgtcc aattattttt acaaggtcaa
    15661 acagcagtat cctttgacag aaaaacagca ggagctctat gacattttag gagatgttaa
    15721 tcctgagtat gcactcaagt atatgacggc ttttttgttg aaatttctca aaaaagacca
    15781 gcttatgcag aaatgccgtg atatctttgt ggacagtttg gttgtcttag gctatattgt
    15841 gcaaaatgaa gatagaaagt atgagttggc tatcgatttt gataaggaga gattaacttt
    15901 ctacttagcg tgatttcttg tttctgagca cattgtttga ctttccttag tattcggtat
    15961 aaactatatg taaccggtaa cacatatcgg aataaactaa aggagacaat catatgtcac
    16021 ttgaaaacaa attggaacaa gcaacaggcg ctgtcaaaga aggttttggt aaagttactg
    16081 gagacagcaa gacagaactt gaaggagctg ttgaaaaaac agttgctaag gcaaaagacg
    16141 ttgtagaaga cgcaaaaggt gctgtagaag gtgccgttga aggtttgaaa aacgttttta
    16201 ctaaataata ggaaaaaatc aagggtttca ttttcccttg attttttcta ttcttataaa
    16261 taattttctg cgacggctgt atctcctggg taggattctt tcttgccctg gatgatttgg
    16321 taacaatcgg ctcccttacc cgcaataata actgcatcta attcgtgatt tgtgatagcc
    16381 attgccgcct tgatggcttc ttggcgatcc gcaatctttt caacaggatg attgatgtag
    16441 ctactaattt catctgcaat ggccattggg tcttcatagt tagggtcatc agcagtcaga
    16501 aagacttgaa tctcagggtg ttgattgagg aggaggccaa agtccttacg acgactttct
    16561 cccttgtttc ctgttgatcc cagaaccaga gcaatctttc cggtttgatg agtttcaacc
    16621 acattgatga gttttttcag actatcccca ttgtgggcat agtcgatgaa gaccttggct
    16681 ccatttttct gagtgaggac ttccatacga ccaggaacgc gggttgcagc gatgcctttt
    16741 ttgatgtcct caagacttgc tccgagacgg agacaagcaa gtccagcagc aactgcattt
    16801 tcttggttga agttgccaat gagttggata tcataatctc cagcgagttt acccgtagct
    16861 gaaaagctaa aggctttgga attctcgatt tggttatcaa attggctacc atagaaatca
    16921 tggtcttgat cttcaacctg ttctttcaag actgagaagt ggtccatgtc actgttaatg
    16981 atgactgctc ggctattttc catcaagaga cgcttgtggt agaaatagtc ttcaaagcta
    17041 gggtgttcaa tcgggccgat atggtcagga gtgatgttaa gaaagactcc tacatcaaag
    17101 gtcagtccat agactcgatg gactagatag gcttgactgg agacttccat gatgaggtgg
    17161 gtacggtcat tttgcacagc ctgattcatc atgtcaaaga ggtcaatact ctcaggggtt
    17221 gtcaacgctg acttaaagaa agtctcgcca tcaagagttg tgttcatggt cgacaacata
    17281 gcaggtctat gcccttgaga taagatgtta taggcgaaat aggttgctgt tgtcttaccc
    17341 ttagtaccag taaaggcaag gagtttgagt ttctcttgtg gattaccata gaactccatg
    17401 gcaatcaaac tcatggcttt ctttatatcg ttcacaatga tgacagggat atcgacttcg
    17461 tagtcctttt cagctacata ccaagctaaa ccttgtgtta tagcagaaag aaggtattct
    17521 tttttaaagg cagcgccttt tgcaaaaaaa agagtgtctt ctgttacttt tcggctgtcg
    17581 tagctgatgc tatcaaaaat aactttgctg tagttgtagt ggtaatgacc ttggtcaata
    17641 atttcgcgaa aaaggccatc tttctttaaa atatctaata cggtttcaat cttaatcata
    17701 ctttctattg taaaccgaaa gtcgtaaatt tacaagtaac aaggaaaagt ttataatgga
    17761 agataaggag tttttcctag ttatcaaaat tgaatgagga atctatgtcg cacgaaaaca
    17821 atcaccagca ggcccagatg ttacggggga ctgcttggct aacggctagt aactttatca
    17881 gtcgcctact cggggctgtt tacattatcc cttggtacat ctggatgggg gcttatgcag
    17941 ctaaggcaaa tggtctcttt accatgggtt acaatatcta tgcttggttc ttgttggttt
    18001 caacagcggg gattccagtt gcggtggcca agcaagttgc caagtataat accatgcgag
    18061 aagaagagca tagctttgcc ctgattcgga gcttcttagg ctttatgaca ggactaggcc
    18121 tggtttttgc tttagtcttg tatgtctttg ctccttggct agcagacttg tctggcgtgg
    18181 gcaaagactt gatcccaatc atgcaaagct tggcttgggg agtcttgatt ttcccgtcta
    18241 tgagtgttat ccgaggattt ttccaaggga tgaataacct caaaccctat gccatgagcc
    18301 aaattgctga gcaggtcatt cgtgttatct ggatgctcct agcaaccttt atcattatga
    18361 agctcggttc aggagattat ctagcagccg ttacccaatc aacctttgct gcctttgtcg
    18421 gtatggtagc cagttttgca gtcttgattt atttccttgc ccaagaaggt tcactcaaaa
    18481 gagtctttga aacaggagat aagattaaca gtaagcgtct cttggttgat accattaagg
    18541 aagccattcc ttttatcctg acagggtctg ccatccagct cttccagatt ttggatcagc
    18601 tgacctttat caatagtatg agctggttta ccaactacag caatgaggac ttggttgtca
    18661 tgttttctta tttctcagcc aatcctaata aaatcacgat gattttgatt tctgtagggg
    18721 tttcgattgg gagtgttggt ttgccacttt tgacggaaaa ctatgtcaag ggggacttga
    18781 aagcagcttc tcgtctcgtt caggacagtc tcaccctact ctttatgttc ttgctaccag
    18841 caacggttgg agtggttatg gtaggagaac ctctttatac ggtcttctat ggtaagccag
    18901 atagtttggc tctgggctta tttgtctttg cagttttgca gtctattatt ttaggcttgt
    18961 acatggtctt gtctccaatg cttcaggcca tgttccgcaa ccgcaaggcc gttctctatt
    19021 ttatctatgg ttctattgcc aagctagtct tgcaactacc taccatcgcc ctcttccaca
    19081 gttatggtcc tttgatttca acaaccattg ctctcatcat tcctaacgtc ttgatgtatc
    19141 gggatatttg taaagtaact ggtgtcaagc gcaaggtgat tttgaagcga accattttaa
    19201 tcagtttgct gaccctagtc atgtttctgt taataggaac catccagtgg ctgttaggat
    19261 ttttcttcca accaagtgga cgtttgtgga gcttctttta tgtagctctt gtcggtgcca
    19321 tggggggtgg actttatatg gttatgagtc tgcgtaccta tttattagat aaggtaatag
    19381 gaaaagccca agcagatcgc ctgcgagcaa aatttaagct ttcgtaaggt agcatttata
    19441 aataaaagga ataagttgaa caatctttta ggagaacttg aagcttattc cttttttagc
    19501 gtaaaaatag ctaaaattat atttcaaaaa tagaaaatga ggcttttttc gattttttgg
    19561 aaaaaatcat tgactaaaca aactaaacgt tgtataataa aacaaatatt taaagcagga
    19621 ggttccggaa atgaaaaagt ctaagagcaa atatctaacc ttggcaggtc ttgtcctggg
    19681 tacaggagtt ttattgagcg cgtgtggaaa ttctagcacg gcgtcaaaaa catacaacta
    19741 tgtttattca agtgatccat ctagcttgaa ctatctagca gaaaaccgcg cagcaacatc
    19801 cgatattgtt gcaaatttgg tagacgggtt attagaaaat gaccaatatg ggaatattat
    19861 tccatcatta gcagaggatt ggactgtttc tcaggacggt ttgacctata cctacaaact
    19921 tcgtaaggat gccaagtggt ttacttctga gggagaagaa tatgcgcctg taactgccca
    19981 ggattttgtg acaggtttgc aatatgcagc tgataaaaaa tcagaagcct tgtatctagt
    20041 gcaggactct gttgctggtt tggatgacta tatcactggt aaaacaagcg acttttcaac
    20101 tgtcggtgtc aaggcacttg atgaccaaac ggttcaatat actttggtta aaccagaact
    20161 ttactggaat tcaaaaacac ttgcaacgat actttttcct gttaatgcag atttcctgaa
    20221 atcaaaaggg gatgattttg ggaaggcgga tccatctagt attttgtaca atggaccttt
    20281 cttgatgaaa gcacttgtct caaaatctgc tattgaatat aagaaaaacc ctaattactg
    20341 ggatgctaag aatgtctttg tagacgatgt gaaattgacc tactatgatg gtagcgacca
    20401 agaatcactg gaacgtaatt ttacagctgg tgcttatact acggctcgtc tttttcctaa
    20461 cagctccagc tatgaaggga ttaaagaaaa atacaaaaac aatatcatct atagtatgca
    20521 aaattcaact tcatatttct ttaattttaa cctagatagg aagtcttaca attatacttc
    20581 taaaacaagt gacattgaaa agaaatcgac tcaggaagca gttctcaata aaaacttccg
    20641 tcaggctatc aattttgctt ttgacagaac atcttatggg gctcagtctg aagggaaaga
    20701 aggtgcaaca aagattttgc gtaacctagt ggttcctcca aactttgtca gtatcaaggg
    20761 aaaagacttt ggtgaagttg tagcctctaa gatggtcaac tatggtaagg aatggcaagg
    20821 tatcaacttt gcggatggtc aagaccctta ctacaatcct gagaaagcca aggctaagtt
    20881 tgcggaagct aagaaagaac tcgaagcaaa gggtgttcaa ttcccaatcc acttggataa
    20941 gactgtggaa gtaacagata aagtaggcat acaaggagtt agttctatca aacaatcaat
    21001 tgaatctgtt ttaggttctg ataatgtagt gattgacatt cagcaattaa catcagatga
    21061 gtttgacagt tcaggctact ttgctcaaac agctgctcag aaagactatg atttatatca
    21121 tggcggttgg ggacctgatt atcaagaccc gtcaacctat ctcgatattt ttaatactaa
    21181 tagtggagga tttctgcaaa atcttggact agagcctggt gaggccaatg acaaggctaa
    21241 ggcagttgga ctggatgtct atactcaaat gttggaagaa gctaataaag agcaagatcc
    21301 agccaaacgt tatgagaaat atgctgatat tcaagcttgg ttgattgata gttctttagt
    21361 tcttccaagt gtttcgcgtg ggggaacacc atcattgaga agaaccgtac catttgctac
    21421 tgcctatggt ttaaccggta caaaaggggt tgaatcatat aaatacctca aagtacaaga
    21481 taagattgtc acaacagacg aatatgcaaa agccagagaa aaatggttga aagaaaaaga
    21541 agaatccaat aaaaaagccc aagaagaatt ggcaaaacat gtcaaataaa atctaattgt
    21601 agataagttt gtgtaagaag tctttgtgct taaaaacgca taatatcagg tatataaaat
    21661 cctagatgtt atgcgttttg ttataggaag atttattaga ttttatcctc aaattgagtt
    21721 attcccgact ttatccgttg aaatattata aggagtattt atgaaattaa aattattaag
    21781 agtagatact aaggtgatta tggggagttt cttacttgtt ctgtctagtc tacttgcttt
    21841 gttgcttccc cttatcttaa agggtttaat agatgggagt tctattgaaa atataggttc
    21901 caaagtattt caatcgtttt tgatttttat tggtcaagcc ttgttttctt ctattggtta
    21961 ctatctgttt agtcaatcgg gtgaaaaaaa gatagcaaaa atcaggaaaa aagtgataga
    22021 ggggttgatt tatgcagaga aatccttctt tgataagagc caaagtgggg agttgacttc
    22081 tgccattgtc aatgacacga gtgtcattcg tgagttttta attacgactt tcccaaatat
    22141 tattctgagt ttagttatgg tacttggttc cattgtagtc ttatttagtc ttgattggaa
    22201 tctttctcta cttttattca tcactcttcc ttgtatgatg tttattatct tgctcctttc
    22261 caatatcagt gaaaagtata gtcgtcgttt acaggaggaa atcggatttt taacagggca
    22321 attgactgaa aagattcaag aacatgaatt aattaagact aaccaagcag agaaaagtgt
    22381 acaggatgtt ttggataatt gtattgaaag ggtacaaaac aattcactga aatcagatag
    22441 ggttacttct tttgagaccc cgtttgctct cttatttatc tttgcgacta tagctgtgat
    22501 gctaacctat gggggttatc gggtcagcgc aggatatata tctgtgggaa ccttggtttc
    22561 gtttttgatt tacctctttc aattacttaa tcctattagt aatatagcta attttgtaac
    22621 tgtttattct aggagcaagg gatcttcagt tgcactggag aacttgcttg cagttcctaa
    22681 agaaaaattt gagtgaggaa aatcggtatc aggacaaggg ttgaatttta accatgtcta
    22741 ttttggttat gatgaaaatc gacctgtctt aaaggatatt acttgttcaa ttttcaaggg
    22801 gcaaaaaatt gcttttgttg gaccatctgg atcaggaaaa tcaacgattg tgcgtttgtt
    22861 agagcggttt tataaaccgc tttcaggaga tattctaatg gagcaatcaa gtatatatga
    22921 ttttaactta aaagaatgga gaagtaaaat cgcttagtaa ctgatgatga attgatgaaa
    22981 gtgctagact tagtatcact aggtgatgag attcgctcca tgaaagaggg actagatact
    23041 gaagttggtg aacgcggacg actcttgtca ggggggcaaa gccaaagact tcaaatagct
    23101 agagcctact taaaagatgc tgaaattctt atatttgatg aagccactgc taatcttgat
    23161 gcggattctg agtatgcgat tatcagtagc ctctattctg tattaaagga gaagacggtt
    23221 gtgattatag cgcatcgttt gtcaacggta aaagatgtgg attgtatttt cttcttagag
    23281 gaggggaaaa tcactggctc aggaactcat aaggaactac tggaaaatca tgagcgttat
    23341 gctcgttttg tgcaggagca aatgatagag tgaaggtgtc ttttgagatt caccatttta
    23401 tagtctatta aagggagcag gaaaaactcc ctttttatat agtttgaaac tataactagc
    23461 tcttgaaaag aagaaaatga gttgatgaaa ataagtggta caatagttac tatagatttg
    23521 gaggtattgt atgagcaagg aattacacat taacacaatt ttggcccagg cgggtattaa
    23581 gtcagatgaa gcgacaggtg cattggtgac accgcttcat ttttcaacga cctatcagca
    23641 tccagagttt ggtcgatcta ctgggtttga ctatacgcgc actaaaaatc caactcgtag
    23701 taaggctgag gaagtcttgg cggctattga gtcagcagac tatgccctag cgactagctc
    23761 agggatgtca gctattgtac tggcctttag cgtctttcca gtaggaagta aggtcttggc
    23821 agtgcgtgat ctttacggtg gttcttttcg ctggtttaac caagtggagc aggaaggtcg
    23881 tttccatttt acctatgcca atacagaaga agagttgatt gccgagttag aaaaggatgt
    23941 ggatgttctc tatatcgaaa ccccaaccaa tcccttgatg ttggaatttg atatcgaaaa
    24001 actagcaaaa ttggctcatg ctaagggtgc caaagtggtg gtggacaata ccttctatag
    24061 ccctatctac caacgtccga ttgaagatgg agcagatatc gttctccatt cagcaaccaa
    24121 gtatctagca ggccacaatg atgtcttggc tggagtggtt gtgaccaata gtttagaact
    24181 atacgagaag cttttttaca atctcaatac aacaggggca gtcttgtctc catttgacag
    24241 ctaccagttg cttcgtggtc tcaagacctt atctcttcgt atggagcgct caacagctaa
    24301 cgcccaagaa gtggttgcct ttttgaagga ttctccagca gttaaggaag ttctctacac
    24361 tggtcgtgga ggcatgattt cctttaaagt agccgatgaa acacgcattc ctcatatttt
    24421 gaacagtctc aaggtcttct cttttgcgga aagtttgggc ggagtggaaa gtcttattac
    24481 ttatccaacg actcaaactc atgctgatat tccagcagaa gtacgccatt cttatggttt
    24541 gacagatgac ctcttgcgtt tgtctattgg gattgaggat gctagagatt tgattgcaga
    24601 tttgcgccaa gccttagaag gataagacaa agatgggaaa atatgatttt acaagcctgc
    24661 ccaaccgttt agggcaccat acctataaat ggaaagaaac agaaacggat agtgaagttc
    24721 taccagcttg gatagcggat atggactttg tggtcttgcc tgaaatccgc caagccgtgc
    24781 aaacttacgc agaccaactg gtttatggtt atacctatgc cagtgaagac ttaattaagg
    24841 aaattcaaaa gtgggaagct acacaatacg gttacaactt tgacaaagag gctcttgtct
    24901 ttatcgaggg tgtggtacca gccatctcaa cagctattca aacctttaca aaagaaggcg
    24961 aggcggtttt aattaacacg cctgtctacc caccctttgc tcgcagtgtc aagttgaata
    25021 atcgtagatt gattactaat tccttagtgg aaaaggatgg tctgtttgag attgactttg
    25081 accaacttga aaaggatttg gtggaagagg aggttaaact ctatattctt tgcaaccctc
    25141 acaatcctgg tggacgtgtt tgggaaaaag aagtgttgga gaagattggc caactttgcc
    25201 aaaaacacgg tgttttgtta gtttcggatg agattcacca agatttgacc ctctttggtc
    25261 acaaacacca gtctttcaat accatcaatc ctgccttcaa aaattttgct atcgtcttga
    25321 gcagtgccac taaaacattt aatattgctg gaacaaaaaa ttcctatgca gtcattgaaa
    25381 atcctaagtt gagactggct ttccagaaac gcctgttggc caataatcag catgaaattt
    25441 caggcttggg ttatttggcg acagaagctg cctatcgata cggtaaagat tggctagagg
    25501 aactcaagca agtctttgaa gaccacatca attatgtggt ggatctattt ggaaaagaga
    25561 ctaaaatcaa ggtcatgaaa ccgcaaggta cctacttgat ttggcttgac ttttcagcct
    25621 atgacctgac tgatgaaaca ttgcaagagt tgttgagaaa tgaagccaag gttatcctca
    25681 accgtggttt ggattttgga gaggaaggaa gtctccatgc ccgcatcaat atagctatgc
    25741 ccaaatctct gttgcaagaa gtctgtcagc ggattgtggc tacttttgcc aaacgttaaa
    25801 aatccagcct tctaggagaa aagtcttcct agaaggctat tttcataggc gaaaatatgg
    25861 tataataaaa agataaggta aaggtgaaaa tatggctaaa ttgattccgg ggaaaattcg
    25921 tatcgaaggt gttgcccttt atgaaactgg taaggttgat atcatcaagg aaaagaacaa
    25981 tcggctctac gctcgcgttg cagaagaaga actgcgctat agtttagagg atgatttggt
    26041 tttttgtgcc tgtgattctt ttcaaaagag gggctactgt gtgcatttgg cagcgctaga
    26101 gcattttctg aaaaatgatg agcgtggtca ggaaatcttg tggagtctgg aagaaggtca
    26161 tgaagaaaaa gaggccgttg aaaccaaggt gaccttgggt ggcaagtttt tgaatcgaat
    26221 tttatctcca aaatcagaat gcgcctatga gttatcagct gtgggtcagg tggaagcagg
    26281 aaccaatcat atcttgtgga ctctgagaat cggccaaatc aatagccaaa aatactatgt
    26341 cattcgggat attccacttt ttttaaggat tgtcgagcag agaaagtcgt atatgattgg
    26401 caagatttat gaggagtctc tttcttggga agcctttgat gatgccagcc aagaactttt
    26461 gactttcttg cacggactaa cggaggaagg gctggttcca gacctctttt tccaaaacca
    26521 aggtcggcac ctctttttcc ctctgacctt ttttgagcag ggtgtgaatt tactgatggc
    26581 cttgccccat tttcaatttg accatcaagt ggatagctac caaaccctgc tttttcagga
    26641 tatgcatgct gatgccaatc tatttgcctt tacagtaaca gaatactcgg actattttga
    26701 aatggaaatc agtgagagtc ctagggtcaa tgtcttttat cagggagcag tacttttcca
    26761 taaaggtcag gtttattttt taacagacca gcagatgcgt cttctcaagg aaattaaagc
    26821 gttacctgtg ggtcaacaag gaaagaaata cctgcaattt gatagcagtg atcgagacaa
    26881 attggcctcc tgtctgactc tttttatcca gatgggcacc gtttcagctc cagaacgtct
    26941 acaaatcaaa acttttgcgc cctcttttta ctttgacagg gaagaagata atcggattcg
    27001 tttagaaatt cagtttgatt atggaaatag acaggtatct agccgacaag agctagaaga
    27061 attaccattt tcaagtgacg cggacttaga agaaagaatt ttccaagtct gtttggctgc
    27121 gggctttgaa gccgattttc aatcttggcg tcaggcctta aaagcggagt ctgtctatca
    27181 tttttttcat gatatcattc cagtctttga aaaactcggg catgttgacc tatcagataa
    27241 actagaagag ctttatagcc tagcgagtcc tcaggtgcag attgcctcca agggaggtct
    27301 cttggaaatc cagtttgatt ttcaaaatat tgcccaggag gaaatcgacc aagccatgca
    27361 ggccttggtt gccaatcagg atttttatat tgattcgtct aatcaagttt actttttcga
    27421 tgaagaaacc aagaaaattc gccaaaatct acaggaactg gggcaatttg aattaaaaga
    27481 tgggaccttg caggctcgga aatccttggc ctacagttta gctcatctct ttgaagggcg
    27541 agaccgtgtt tctttttcac aagaattcca gaatctggcc caggatttga cgcatccaga
    27601 ggactttcct ttgcaagcaa ctcaggtcaa ggctgacttg cgagattatc aggaaaaggg
    27661 aattggctgg ttgcagatgc tccatcatta tggttttggt gggattttgg ctgatgatat
    27721 gggacttggg aaaacccttc agaccattgc ttttttgact agtcaagtga caaaagaaag
    27781 tcgagtcttg attctcgctc cgtcgggttt gatttacaac tgggcggatg agtttcagaa
    27841 atttgcccca cagttggatg tggctgttgt tcatggtttg aaagcaagtc gtgaggagat
    27901 tcttgccgag agccatcaaa tctatgtgac tagctatgcc accttccgtc aggacagtga
    27961 gttttatcag gggatggcct ttgacttcct tttcttagat gaggctcagg tcatgaaaaa
    28021 tgcccagacc aagattgccc agaccttgag acaatttgtg gtgccgtcgg tctttgcctt
    28081 gtcaggaact ccgattgaaa accatctggg tgagttgtgg tctattttcc aaatcgtcat
    28141 gcccggactt ttgccaagca agaaagaatt tatgaaattg ccagcagagc gagtggctca
    28201 gtttatcaag cctttcgtga tgcgacgcaa gaaagaagaa gttctgactg aattgccaga
    28261 cttgattgag gtggtttata aaaatgaact ggaagaccag caaaaggcta tttaccttgc
    28321 ccagttacaa cagatgcgag accatctggc tcaagtgtca gaacaggaat ttcagcgaag
    28381 tcgtgtggaa attttatctg gtttgatgcg cttgcgtcaa atatgtgaca ctcctgccct
    28441 gtttatggaa gattatcagg gagccagcgg caaactagat agtctccgag acctactggt
    28501 acaggtggca gacggcggac accgtgtctt gattttctct cagttcaagg gaatgttgga
    28561 aaaaattgaa caagaactgc cagacttggg cctgacttcc tttaaaatta cgggttcaac
    28621 cccagccaag gaaagacaag acatgaccaa ggcctttaat caaggagaaa gagatgcctt
    28681 tctgatttcc ctcaaggctg gtggggtcgg tctgaacctg acaggtgcag atacagtgat
    28741 cttggttgac ctttggtgga atcctgcggt ggaagcgcaa gccattggcc gtgcccatcg
    28801 gatgggtcag gaagaaacgg ttgaggtcta tcgcttggtg accaagggaa ccattgaaga
    28861 aaaaattcag gaactccaag aacaaaagaa acatctggtg tcacaagtat tggacggcac
    28921 agagtcacgt ggtagtctga ccctagcaga aattagagaa attttgggaa tttctgaagc
    28981 cagcacttga aaaatcaaga caatacgcta taatgaagtg ttgaaaggaa atagaaaatg
    29041 aaacaagaac gatttccatt ggtgtcagat gacgaggtca tgttgactga aatgccagtc
    29101 atgaatctct atgatgagtc tgatctgatc agtaatatca agggtgagta tcgagataaa
    29161 aattatttag aatgggctcc tattgctgaa gaaaaaccag taaaaccgat tgaaaagcaa
    29221 gtagggaaac ctaaaaaagc tcctttaggg gttaaaaaag aaggaaagag ctatgcggag
    29281 gtggcgcgtg aagaagcgcg tgcagacttg aaaaagaaac gctctgctaa ctatctaact
    29341 caggatttca gccttgcgag acgtcattct cagcccagtc tagttagaca gggcaatcaa
    29401 ccgacaactc ctttccaaaa ggaaaatcct ggtgaatttg tcaaatatag ccaaaaattg
    29461 acccagtctc attatatctt ggcggaagaa gttcattcta tccctaccaa gaatgaagaa
    29521 gtgtcagcac ctgctccaaa gaaaaacaat tatgattttc taaagaagag ccaaatctac
    29581 aataaaaaaa gtaaacaaac agaacaagaa cgtcgggttg cccaagagtt gaatctgacc
    29641 agaatgacag aatagggaga aaacatgtca aagacatatc attttatcgg aattaaggga
    29701 tcagggatga gtgccttggc cttgatgttg caccagatgg ggcacaaggt tcagggatca
    29761 gatgttgaaa agtactactt tacccaacgc ggtcttgagc aggcaggaat taccattctt
    29821 ccttttgatg aaaagaatct agacggtgat atggaaatta tcgctggaaa tgcctttcgt
    29881 ccagataaca acgtcgaaat tgcctatgcg gaccaaaatg gtatcagcta caaacgttac
    29941 catgagtttc taggtagctt tatgcgtgac tttgttagca tgggagtagc aggagcacat
    30001 ggaaaaactt caacgacagg tatgttgtct catgtcttgt ctcacattac agataccagc
    30061 ttcttgattg gagatgggac aggtcgtggt tcggccaatg ccaaatattt tgtctttgaa
    30121 tctgacgaat atgagcgtca cttcatgcct taccacccag aatactctat tatcaccaac
    30181 attgactttg accatccaga ttatttcaca agtctcgagg atgttttcaa tgcctttaac
    30241 gactatgcca aacaaatcac caagggtctt tttgtctatg gtgaagatgc tgaattgcgt
    30301 aagattacgt ctgatgcacc aatttattat tatggttttg aagctgaagg caatgacttt
    30361 gtagctagtg atcttcttcg ttcaacaact ggttcaacct tcaccgttca tttccgtgga
    30421 caaaacttgg ggcaattcca cattccaacc tttggtcgtc acaatatcat gaatgcgaca
    30481 gccgttattg gtcttcttta cacagcagga tttgatttga acttggtgcg tgagcacttg
    30541 aaaacatttt ccggtgttaa acgtcgtttc actgagaaaa ttgtcaatga tacagtgatt
    30601 atcgatgact ttgcccatca tccaacagaa attattgcga ccttggatgc ggctcgtcag
    30661 aaatacccaa gcaaggaaat tgtagcagtc tttcaaccgc atacctttac aagaaccatt
    30721 gccttgttgg acgactttgc ccatgcttta aaccaagcag atgctgttta tctagcgcaa
    30781 atttatggct cggctcgtga agtagatcat ggtgacgtta aggtagaaga cctagccaac
    30841 aaaatcaaca aaaaacacca agtgattact gttgaaaatg tttctccgct cctagaccat
    30901 gacaatgctg tttacgtctt tatgggagca ggagacatcc aaacctatga atactcattt
    30961 gagcgtctct tgtctaactt gacaagcaat gttcaatagg atgttcccat ggaaattcca
    31021 attaagatca ttcaggcaag caagtctgat ttgcctgaga taggggcact tcagacctcg
    31081 tcttttccag ctgaaaagca gcaactttcc catattttag aagaaagtat ccgtaagtgt
    31141 gcggatacct ttcttctagc tagggatgaa aatcaacttt taggctatat tttatcaagt
    31201 ccccagtcag acaatccgca atatctaaaa gtacattctt tagtcatcga gtctgaccat
    31261 cagagacagg gcttgggaac acttcttctt gcagccttga aagaggtggc agttgatctg
    31321 gattacaaag ggattcgttt ggagagtcct gatgagctgc tttcctattt tgaaatgaac
    31381 ggttttgttg atgaagaagc aactttgctc tatgcaacta gccagggcta tagtatgatt
    31441 tggtttaatc ccttttatct ggaggaacaa tgaaaatcag acaagcaaga ttagaagatt
    31501 tggatcggat tgttgaactt gaatttgaaa atttttcggt cgaagaagcc attcctcctt
    31561 ctgtctttga agcacatttg agagaaattc agacctcttt tctggttgct gaaaaagaag
    31621 gaagaatcat gggttatatc gaaggaccag ttggcctgca ccgccatctg caagaccagt
    31681 cttttacaga agaaataaaa gactatagtc atgagcctgg tggttatatc tctgtgacct
    31741 gtctgtctat tgccaaggag gcacagggac tcggactggg tcagaaattg ctgacagctt
    31801 tgaaagaagt tgctcttgaa gatgagagag atggcattaa tctaacctgt catgactatc
    31861 tcatcgccta ttatgaaaaa catggatttg tcaacgaagg ccagtcccag tcaacctttg
    31921 caggggaaac atggtatgat atggtctggg aaatgaaaaa ataagttagg aaaagtatca
    31981 tagattgctt tttttggact tttaagatat aatattatgg attatcaaca aggaggaaaa
    32041 acttttgagt gaaaagtcaa gagaagaaga gaaattaagc tttaaagagc agattctgag
    32101 agatttagaa aaagtaaaag gctatgatga agttctgaaa gaagatgagg cagtagttcg
    32161 cactcctgca aatgaacctt cagctgaaga actcatgact gattccttgt caacggtaga
    32221 ggagattatg agaaaagctc ctaccgtgcc tactcaccca agtcaaggtg taccagcttc
    32281 tccagcagat gagattcaaa gagaaactcc tggtgttcca agtcatccaa gtcaagatgt
    32341 accttcttct ccagcggaag aaagtggatc aagaccaggt cctgttagac ctaagaaact
    32401 tgaaagagaa tacaatgaaa ccccaacaag ggtagctgtt tcctatacga cggcagagaa
    32461 aaaagcagaa caagcaggtc cagaaacacc tacgcctgct acagaaacag tggatatcat
    32521 cagcgataca tcacgtcgta gccgtagaga aggagcaaaa cccgttaatc ctaagaaaga
    32581 gaagaagtca catgtgaaag cttttgtgat ttcattcctt gtattccttg ccttgctctc
    32641 agcaggtggt tactttggtt accagtacgt gctagattct ttgttaccta tcgatgctaa
    32701 ttctaagaaa tatgtgacgg ttggaattcc agaaggttca aacgttcaag aaatcggtac
    32761 gacgcttgaa aaagctggtt tggtaaagca tggtctgatt tttagttttt atgccaagta
    32821 taaaaattat accgacttga aagcaggtta ctacaatttg caaaagagta tgagtacaga
    32881 agacttactc aaagagttgc aaaaaggtgg aacagatgaa ccgcaagaac ctgtacttgc
    32941 gactttgaca attccagaag gttatacctt ggatcagatt gctcaagctg tgggtcaatt
    33001 gcaaggtgac ttcaaagagt ctttgacagc ggaggctttc ttggctaaag ttcaagatga
    33061 gacgtttatc agtcaagcag tagcgaaata tcctacttta ctggaaagtt tgcctgtaaa
    33121 agacagcggt gcgcgttatc gtttggaagg ataccttttc ccagctacat actctatcaa
    33181 ggaaagcaca actattgaga gcttgattga tgagatgtta gctgctatgg ataagaacct
    33241 atctccttac tatagtacta tcaaatctaa aaatttgact gtcaatgagt tgttgaccat
    33301 tgcttccttg gtcgaaaaag aaggtgccaa gacagaagat cgtaagctca ttgcaggtgt
    33361 attctacaat cgtttaaatc gtgatatgcc acttcaaagt aatattgcaa tcttgtatgc
    33421 ccaaggaaaa ctggggcaaa atatcagtct agctgaggat gttgcgattg ataccaacat
    33481 tgattcacct tataatgttt ataaaaatgt aggtctcatg cctggtccag tcgatagtcc
    33541 aagtctggat gcgattgagt caagcatcaa tcaaactaag agcgataacc tctactttgt
    33601 agcagatgtc acagaaggca aggtctacta tgctaacaat caagaagacc acgaccgcaa
    33661 tgtcgctgaa catgtcaaca gcaaattaaa ctaaacaaac taaaattatg tgatacgtca
    33721 cataattttc tttagaaaat attatcagaa gaaagttgag aaaaatggca gaaaaaacat
    33781 atcctatgac ccttgaggaa aaggaaaaac ttgaaaaaga attagaagaa ttgaaattgg
    33841 ttcgtcgacc agaagtggta gaacgcatta agattgcccg ttcatacggt gacctttcag
    33901 aaaacagtga gtacgaagca gctaaggatg aacaagcctt tgtcgaagga caaatctcta
    33961 gcttagaaac aaaaatccgc tatgctgaaa tcgtcaatag cgacgcagtt gcccaggacg
    34021 aagtagcgat tggtaaaaca gtcaccatcc aagaaattgg tgaggacgaa gaagaagttt
    34081 atattatcgt aggttcagct ggtgcggatg cctttgcagg taaggtttca aatgaaagcc
    34141 caattgggca ggccttgatt ggcaagaaaa caggtgacac agcaaccatt gaaacgcctg
    34201 ttggtagcta tgatgtaaaa atcttgaagg ttgaaaaaac agcctaaaaa cagaaaaagg
    34261 agtggggagg cgatgtgctt cactcactcc tttttccatt ttgctattct tcgaaaatct
    34321 cttcaaacca cgtcagcgtc gccttgccgt atgtatggtt actgactttg tcagtttcat
    34381 ctacaacctc aaaac
//
LOCUS       DAVBCV010000018        33324 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBCV010000018 DAVBCV010000000
VERSION     DAVBCV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.018"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..327
                     /locus_tag="VQ516_000840"
     CDS             <1..327
                     /locus_tag="VQ516_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2511308.1"
                     /translation="KPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQV
                     ETEKVEEKMREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLA
                     LLKESK"
     gene            749..979
                     /locus_tag="VQ516_000841"
     CDS             749..979
                     /locus_tag="VQ516_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET2511309.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            1383..2096
                     /locus_tag="VQ516_000842"
     CDS             1383..2096
                     /locus_tag="VQ516_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2511310.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2154..2756
                     /locus_tag="VQ516_000843"
     CDS             2154..2756
                     /locus_tag="VQ516_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511311.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            2892..3545
                     /locus_tag="VQ516_000844"
     CDS             2892..3545
                     /locus_tag="VQ516_000844"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2511312.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3555..4019
                     /locus_tag="VQ516_000845"
     CDS             3555..4019
                     /locus_tag="VQ516_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2511313.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4032..5300
                     /locus_tag="VQ516_000846"
     CDS             4032..5300
                     /locus_tag="VQ516_000846"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2511314.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5314..5574
                     /locus_tag="VQ516_000847"
     CDS             5314..5574
                     /locus_tag="VQ516_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511315.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5585..6946
                     /gene="pdrM"
                     /locus_tag="VQ516_000848"
     CDS             5585..6946
                     /gene="pdrM"
                     /locus_tag="VQ516_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2511316.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7100..8068
                     /locus_tag="VQ516_000849"
     CDS             7100..8068
                     /locus_tag="VQ516_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2511317.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8084..9076
                     /locus_tag="VQ516_000850"
     CDS             8084..9076
                     /locus_tag="VQ516_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2511318.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9294..10337
                     /locus_tag="VQ516_000851"
     CDS             9294..10337
                     /locus_tag="VQ516_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2511319.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10401..12086
                     /gene="lpdA"
                     /locus_tag="VQ516_000852"
     CDS             10401..12086
                     /gene="lpdA"
                     /locus_tag="VQ516_000852"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2511320.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEIIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMQSPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEIIGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12150..13139
                     /locus_tag="VQ516_000853"
     CDS             12150..13139
                     /locus_tag="VQ516_000853"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2511321.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQ516_000854"
     CDS             complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQ516_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2511322.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14860..16410)
                     /locus_tag="VQ516_000855"
     CDS             complement(14860..16410)
                     /locus_tag="VQ516_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2511323.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFIIIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGSLVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(16426..17205)
                     /locus_tag="VQ516_000856"
     CDS             complement(16426..17205)
                     /locus_tag="VQ516_000856"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2511324.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKHKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(17192..18043)
                     /gene="ylqF"
                     /locus_tag="VQ516_000857"
     CDS             complement(17192..18043)
                     /gene="ylqF"
                     /locus_tag="VQ516_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2511325.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EGLDGND"
     gene            complement(18250..18426)
                     /locus_tag="VQ516_000858"
     CDS             complement(18250..18426)
                     /locus_tag="VQ516_000858"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2511326.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(18478..24369)
                     /gene="iga"
                     /locus_tag="VQ516_000859"
     CDS             complement(18478..24369)
                     /gene="iga"
                     /locus_tag="VQ516_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HET2511327.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGSIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDAYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DQDAHIQNSYAEGDINNVKHFGKVAGVAGYLWDRTSGEEKHAGELTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     NPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPEVKLTELY
                     LEDQFSKTKQNLGDSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     QHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALN
                     DFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEA
                     KFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRNGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVL
                     DPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKMAALRKIENKYVKDP
                     ADGNEVYATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAP
                     IYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYDPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(24578..25756)
                     /locus_tag="VQ516_000860"
     CDS             complement(24578..25756)
                     /locus_tag="VQ516_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511328.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCRNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(25926..29576)
                     /gene="addA"
                     /locus_tag="VQ516_000861"
     CDS             complement(25926..29576)
                     /gene="addA"
                     /locus_tag="VQ516_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2511329.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWKLAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGDQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALVEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(29573..32848)
                     /gene="rexB"
                     /locus_tag="VQ516_000862"
     CDS             complement(29573..32848)
                     /gene="rexB"
                     /locus_tag="VQ516_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2511330.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(33048..33203)
                     /locus_tag="VQ516_000863"
                     /pseudo
     CDS             complement(33048..33203)
                     /locus_tag="VQ516_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         9516 a         6538 c         6861 g        10409 t
ORIGIN      
        1 aaacctcaga cagaaaaacc tgaggaagaa acccctcgag aagagaaacc gcaaagcgag
       61 aaaccagagt ctccaaaacc aacagaggaa ccagaagaag aatcaccaga ggaatcagaa
      121 gaacctcagg tcgagactga aaaggttgaa gaaaaaatga gagaggctga agatttactt
      181 ggaaaaatcc aggatccaat tatcaagtcc aatgccaaag agactctcac aggattaaaa
      241 aataatttac tatttggcac ccaggacaac aatactatta tggcagaagc tgaaaaacta
      301 ttggctttat taaaggagag taagtaaagg tagcagcatt ttctaactcc taaaaacagg
      361 ataggagaac gggaaaacga aaaatgagag cagaatgtga gttctagttc tcattttttt
      421 catgaaaatg tgcaaaatat agtagattga aactagaata gtatacctct acttctaaaa
      481 cattgttaga aatcgatttg actgtcctgt tcttatttca ttttactata tcttaacaga
      541 tagtgtaaat aaagataaac tatttactgg ctaattaatc agttaaacac tagttaagga
      601 gtaatgatga aaaaaagaac aatactatta ttgatggcca gtctgttagc tcttgtctta
      661 ggagcatgtg gtttcttgga catattgatc ctggatcatt ctcatcagga ttactcttta
      721 ctgctatttt agaaactggg gtggtttgat ggaaagtatt ggtattggtc ttgttatcgt
      781 ttcacattcc aaacacattg cagaaggtgt tgttgaactg attagtaaag tagctaaaga
      841 tgttccgatt acttatgtag gaggaaccga gggcggagga attggaacga gttttgatca
      901 agtagatagg gttgtttccg aaaatccagc agatacttta cttgcctttt ttgacctagg
      961 ttctgctata aaatgttaag taaatgattt caaaatcaga aagggattgc tttgggcagt
     1021 tcctttttaa tgtaaatgta aaaggagtag aatcatagtg tttctaaatt gtgaatcaat
     1081 caaaactgat tgtgatgggg ctattctagc tttagaatcc ttcaaaaatt aaaatttaag
     1141 gcaatcaatt acttggaaga gtatgaaagc atttagttta taggaattct aggcctagaa
     1201 ttacatatgt atttatgaag ataggggtgt tcgatagtta gtattgttct gttctgaaag
     1261 atttgagctg tcagttgtat agaaagtgtt cgaatttttt ttaagtgatt aaattagtta
     1321 attgtatgag gttctttatg atataatgtt cttaatgaat tttcagaaag gaaaacctca
     1381 aattgttcta caaatttcta ctcttcgacc tcgaccacac tcttcttgat tttgatgctg
     1441 ctgaggatgt ggctttgacc caacttctaa aagaagaagg agttgcggat attcaggctt
     1501 ataaagatta ttacgttcct atgaacaagg ctctctggaa agacttggag ctgaagaaaa
     1561 tcagtaaaca agagctggtt aacacgcgct tttctcgttt atttgctcat tttggacagg
     1621 aaaaagacgg tagttttctt gcccagcgtt accaatttta cctcgcccag cagggacaaa
     1681 cactatcggg cgctcatgat ctcttggaca gcctcattga gcgtgattat aacttgtatg
     1741 ctgcgacaaa tggcattact gccattcaga caggacgttt ggctcaatct ggtctagcac
     1801 cttatttcaa tcaagtcttt atctcagaac agttgcaaac tcaaaagccg gatgctctct
     1861 tttatgaaaa gattggccag caaattgctg gatttagtaa agaaaagacg ctgatgattg
     1921 gagattctct aaccgccgac attcaaggtg gcaataatgc ggggattgat actatctggt
     1981 ataatcctca tcacctcgaa aatcacacac aagcccagcc gacttacgaa gtctattctt
     2041 accaagactt gctggattgt ttagataaaa atattcttga aaagatcaca ttttaaagga
     2101 gacgagctaa tgactacaaa aaagctaata ttactattga agagtacatt gaaatgtctg
     2161 aagttgattt taatgaagct gttaattatg aatttacatc tgacacttgt caattagcaa
     2221 atagtattta tcaatctctt tttaagtttt ttgataagaa aaatttctct ggcgatttaa
     2281 tttttacttg gaaatctcca tcattagtca aagaagggga ttatattggg agaagggatt
     2341 cacaagtaga taatcttaga gtaataggaa atatatttcc gaattatctt actaatcgaa
     2401 aatatagcct caatatgaat cgtaatggct gtatgggaga ttttcctcat gacttttttg
     2461 atatatacct agatcatgta gcaaaatatg cctacgaaca aaaagttaat aatattaaag
     2521 agtattatcc tttaaaaaga gcgattttac accaagagaa tgcattgtat tttcgatttt
     2581 tttctaattt tgacgacttt ttagaaaaaa attatttaaa gactatatgg caagtttcta
     2641 aagaaactcc tttttctgaa atggatttta atatgtttaa aaatatttca gaaaagataa
     2701 tcttcgaaag aggtagtaaa atgcttaatg atttaaaaag taattataaa aaatagccag
     2761 tctttataaa ctgtattcac gtattcaaat cgtatatagt cgatttatta aatctttaga
     2821 taaactgtaa aaagtggttt ccatagccac tttttgctat aatagaggca gtaaaaatga
     2881 aggagtcggc tatggaacac tcgtcttggc atgctttgat taaggcgcaa ttacctgagg
     2941 gttatttcgg gaaaatcaat cagtttatgg agcaggtcta ttctcagggg attatttatc
     3001 cacccaagga aaaggttttt caggctctct tgacaacact gcttgaagaa gttaaggtgg
     3061 taattctagg gcaagacccc tatcacggac caggtcaagc gcagggcttg agtttttctg
     3121 tacctgactc tatcccagct ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg
     3181 atatcggagt taagaaatct catgatttga cagcttgggc tgagcaagga gtcttgcttc
     3241 ttaatgcttg tttgactgtt cctgctggac aggccaatgg tcatgctggt caaatctggg
     3301 agccttttac tgatgctgtg attcaggtgg tcaatcatct agatagacca gtcgtttttg
     3361 tactctgggg agcttatgca cgtaagaaga aggccttagt taccaatcct catcacttga
     3421 ttatcgaatc agcccatcca agtcctttgt cggtttatag aggattttgg ggttccaagc
     3481 ccttttccaa ggccaatgca ttcttaaaag agacaggaca agagccaatc gattggctta
     3541 gataaggaga aaatatgcct cagttagcga cgatttgcta cattgataat gggaaagaac
     3601 tgctcatgct ccatcgtaat aagaaaccca atgatgtcca tgaagggaaa tggattggtg
     3661 tgggtggtaa gctagagaga ggagagacgc cccaggaatg cgcggcgcgt gaaatccttg
     3721 aagaaacagg gctcaaagcc aagccagttc taaaaggtgt catcactttt cctgaattta
     3781 caccagattt agactggtac acctatgttt ttaaggtgac ggagtttgag ggcgacttga
     3841 ttgactgcaa tgaggggacg ctagaatggg ttccctatga cgaggttttg agcaagccga
     3901 cttgggaagg tgaccacacc tttgttgagt ggcttttaga ggataaaccc ttcttttcag
     3961 ccaagtttgt ttatgatggg gataaattgt tggataccca agttgatttc tatgaataaa
     4021 ggagaaagca gatgctacta atcaaaaatg gtcgtgtaat ggatcctaag tctggtttgg
     4081 atcaagtttg tgatgtctta gttcaagatg ggaaaattat caaaattgcg cctgagatca
     4141 cggaagaagg agcagaaacg attgatgcta ctggtcttgt ggttgctcct ggtttggtcg
     4201 atattcatgt gcatttccgt gaacctggtc aaacacataa agaagacatt catactggtg
     4261 ccctggcagc cgctgcaggt ggttttacta ctgtcgtcat gatggctaat accagtccaa
     4321 ccatttcaga cgtggagact ttgcaagcag ttctccagtc agctgccaaa gagaagatta
     4381 atgtcaagac agttgcgacc attactaaaa actttaatgg taaaaacttg actgacttta
     4441 aggcgctctt agaagctggt gcggttggtt tctctgatga cggtattccg cttgagagca
     4501 gtaagattgt caaggaagcc atggaggaag ccaaaaaact caataccttt atcagccttc
     4561 atgaggaaga tccaggtttg aacggtgttc ttggctttaa tgaaaatatt gctagagaac
     4621 atttccatat ctgcggtgct actggggtgg ctgagtacgc tatgatggcg cgtgatgtca
     4681 tgattgccta tgcaactaaa gcccatgttc acatccagca tttgtctaag gaagaaagtg
     4741 ttaaagtagt ggagtttgct caggggttag gtgcagaagt cacagcagaa gtagcgccac
     4801 agcatttctc taagacagaa gcacttcttt taacacaagg tagcaatgct aagatgaatc
     4861 caccgcttcg tttggaatca gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca
     4921 tcacagttat tgcgactgac cacgcgcctc atcatgtaga tgaaaaaaat gttgaggata
     4981 ttaccaaagc gccatctggt atgactggct tagaaacatc cctgtctctc ggcttgacct
     5041 atttagtaga agctggtgag ttgagcttga tggaattact tgaaaaaatg acatacaacc
     5101 cagccaagct ttacaacttt gaagcaggtt acttggctga gaatggtcca gcagatatca
     5161 ctatttttga tgccaaggct gaccgccttg tggactccca ttttgcttcc aaagcagcta
     5221 attcaccatt catcggtgaa accttaaaag ggcaggttaa atataccatc tgtaagggac
     5281 aaatcgtcta tcaagcttga taggagttag aatatgaatt attttcgaaa acgtagggag
     5341 agacaagcga aatctaatag tggaatttac tgtcccgcag ctaatcgttc caaaattcaa
     5401 tatgaaacta aagaaaaagc tgaccatgcg gttcaatatg ataacggagg tttagttaga
     5461 agctattatt gtcgtacatg tgcgtgttgg catactactt caaaatctaa taaacctccg
     5521 ctaagtttga aaaactatgg tctgaaactc tttggtttag ataaatcaga atagaaagag
     5581 aagtatgtat aagacaaagt gtttacgaga gaagttagta ttatttttaa aaattttctt
     5641 cccaatcctg atctaccaat ttgccaatta ttctgcctct tttgttgata ctgcaatgac
     5701 aggtcaatac aacactatgg acttggctgg tgtatctatg gcaaccagta tctggaatcc
     5761 tttctttaca tttctaacag ggattgtgtc agccttggtg cctatcattg gtcaccatct
     5821 tggtcgaggc aaaaaggaag aagttgcgtc tgatttttac caatttattt atttggcctt
     5881 gggcctatct gtggtcttgc tggggatggt acttttcttg gcaccaacaa tcttgaatca
     5941 tattgggtta gaagcagcag tagcggcagt agcggttcgc tatctttggt ttttatctat
     6001 cgggattatc cccttgttgc tctttagcgt cattcgttcc ttgctggatt cgctgggctt
     6061 gaccaaactg tccatgtacc tcatgctttt gttactccct ctcaatagcg gatttaacta
     6121 tctcttgatt tacggtgcct ttggtgttcc agaactggga ggggctggtg ctggtttagg
     6181 aacatccttg gcctactggg tcttgcttgg gatttctgtt ctggttttat ttaaacagga
     6241 gaagctcaaa gccttacacc ttgagaaacg aattccactt aatatggata aaattaagga
     6301 aggagttcgt ttaggtctgc ctattggggg aactgtcttc gcggaagtgg ctatcttttc
     6361 agtggttggc ttgattatgg ctaagttttc gtccttgatt atagctagtc accagtcagc
     6421 tatgaacttt tcaagtctta tgtacgcctt tcctatgagt atctcatcgg ctatggctat
     6481 tgtcgtttcc tatgaagtgg gagccaagcg atttgatgat gcgaaaacct atattggtct
     6541 aggaagatgg actgccctca tttttgcggc cttcacctta accttccttt acatttttag
     6601 gggaaatgtg gccagtcttt atggtaacga cccaaaattt atcgatttga cagcgcgttt
     6661 tttaacttat agtcttttct tccagttagc agataccttt gcggcgccgc ttcagggaat
     6721 tttgcggggg tataaggata cagttattcc tttttacctt ggtttgcttg gttattgggg
     6781 cgtagcaatc cctgtggcta cgctatttga ttccctaaca gattttggag cttattctta
     6841 ctggatcggc ttgattatta gtttgattgt gagtggggcg ctctaccgtt ggcgtttaac
     6901 tgtgattatg aagagatttg aatctttagc aaaatccaaa cgctaaaaag tttgtgaaaa
     6961 aatattcttg ataagaaaat cccttgtttt cacggggttt tcttttttat tttgtgaaat
     7021 ttcttttaga aaaactttga cagtatttgc caaaaacggt aaaatagtaa ggtaagaaga
     7081 aagttagaaa ggcaagaaga tgtcaacttt agataaaaat cttttgctag aaatgttccg
     7141 taagatggaa gaaatccgtc gcatggactt aaaaattgca caattagtaa agaaagggaa
     7201 agtgccagga atgacgcact tttctgttgg tgaagaggca gctaacgtgg gggctatgtt
     7261 agctctcaat ccagatgatc tgattacctc aaaccaccgt ggacacgggc aagctattgc
     7321 taaagggatc gacctcaacg gaatgatggc tgaaatcctt ggtaaataca ctggaacctg
     7381 taaagggaaa ggtggatcta tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa
     7441 tggtatcgta ggtggtggta tggggatcgc tgtcggtgca gccctcagtc agcaaatgca
     7501 aaataccggt aaaatcgttg tctgcttctt tggagatggt gcgaccaatg aaggtgtttt
     7561 ccacgaagca gtgaacatgg cttctatctg gaacctgcca gtcattttct attgcattaa
     7621 caacggttac ggtatctctg cggatatcaa gaaaatgacc aatatagaac atatccatca
     7681 acgtagcgcc gcttatggaa ttcctggaat gttcatcgaa gacggtaaca atgtcatcga
     7741 tgtctatgaa ggatttcaga aagctgtaga ccatgttcgc agtggcaatg gtccagtctt
     7801 gattgaaagt gtaacttatc gctggctcgg tcactcatca tctgaccctg gtaaatatcg
     7861 tacgcgtgaa gaagtggaat tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta
     7921 ccttattgaa aataacattg caagtgccga agaattggaa gaaatccaag cgcaagtaaa
     7981 ggaagcagta gaagcttctg ttaaatttgc agaggaaagc ccattcccac cgcttgaatc
     8041 agcctttgaa gatatttacg cagactaagg agaaagagat aaaatggaaa caaaaacaat
     8101 gtccttccgt gacaccatta tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt
     8161 gttcttgatg ggagaagacg tcggtgtctt cggaggagac ttcggtactt ctgttggaat
     8221 gcttgaagaa tttggtccag aacgcgtccg tgactgtccg atttctgaag ctgccatctc
     8281 tggagcagca gcaggagcag ccatgacagg acttcgtcca atcgtcgata tgaccttcat
     8341 ggacttctct gttattgcca tggacaatat cgtcaaccaa gctgctaaaa cacgttacat
     8401 gtttggtggt aaaggtcagg ttccaatgac tgttcgttgt gcagctggta acggagttgg
     8461 ctctgccgcc cagcactcac aatctctaga gtcttggttt actcacattc caggacttaa
     8521 ggttgtggca cctggaacac ctgcggacat gaaaggcctg cttaagtctt ctatccgtga
     8581 taaaaaccca gttattatac ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc
     8641 agttgatcca gactacacaa ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga
     8701 tgtaacagtt gttacttatg gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt
     8761 agcagaagaa ggaatttcag ttgaaattgt tgacccacgt acccttgttc cgcttgataa
     8821 ggatatcatc ataaactcag tgaagaagac tggtaaggtt gttctggtca acgatgccca
     8881 caaaacaagt ggctatatca gtgaaatttc agctattatt tcagaatcag aagcatttga
     8941 ctatctagat gcaccaatcc gccgttgtgc aggagaagat gtgccgatgc cttatgcaca
     9001 aaacctagaa aatgcaatga ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac
     9061 ttataacaaa gaatagagtt acataagata agttatgtaa ataaaataaa gctgacgaga
     9121 aactttgtat cttttaggtg cagagctctt cgtcattaat atcttatatt cgagcacggg
     9181 ctaaaagctc ggaaaaaaga taaatctcct tggcttcatc gaagccatcg tcgatttcct
     9241 atttttcagt cgcttttgac gccctttgta tcataaatag aattggagag gtcatggctg
     9301 atgacaagct aagagcgact cctgcagcta gaaagttagc ggatgattta gggataaacc
     9361 tctacgacgt ttctggctca ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt
     9421 ataaagacac aaacgtggtt cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata
     9481 acattgcttg gcaggaaatc caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg
     9541 ttttggccct gcttcctgaa aatatcgaaa acgatagcat caaatctcct gctcagattg
     9601 aaaaagtgga agaagtccct gataacgtaa caccatatgg tgaaatcgag cgtattccaa
     9661 tgacaccaat gcgtaaggtt attgcccaac gcatggttga atcttacttg actgcgccaa
     9721 ccttcaccct caactatgaa gttgatatga ctgaaatgtt ggctcttcgt aagaaggttc
     9781 ttgagccaat catggaagca actgggaaga agactactgt aacagacctt ctttcgcttg
     9841 cagttgttaa gactcttatg aaacacccat acatcaacgc ttcattgaca gaagatggca
     9901 agactattat cactcacaac tatgtcaatc ttgccatggc agttgggatg gataatggat
     9961 tgatgacacc tgttgtttac aatgctgaga agatgagtct ttcagaacta gttgtagcct
    10021 ttaaggatgt tattggccgt accttggatg gtaaattggc tccaagtgag ttacaaaatt
    10081 caacattcac aatcagtaat ttgggaatgt ttggtgttca gtcctttggc ccgattatta
    10141 accaacccaa ctcagctatc cttggtgtca gttcgacaat cgagaagcca gttgtcgtca
    10201 atggtgaaat tgtgattcgc ccaatcatga gtttaggatt aaccattgac caccgtgtcg
    10261 tagatggtat ggctggtgct aagtttatga aggacttgaa agagttgatt gaaaccccaa
    10321 tctcaatgtt gatttaagag caagagtatt catttttaag atatagataa aggaaggaag
    10381 acatggcctt agaaataatt atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg
    10441 tccaatggaa taaaaaagtc ggagaatttg taaaagaagg agaaatcctt ttggaaatca
    10501 tgactgataa agtcagcatg gaattggaag ccgaagaaga tgggtacttg attgccattc
    10561 tcaaaggaga tggtgaaact gtccctgtaa cggaaattat cggttacctt ggtgaagaaa
    10621 gggaaaacat cccaacagct ggagcagcat cgccagaagc tagccctgta cctgtagcaa
    10681 gtacctcaaa cgatgatggt aagagcgatg atgcttttga tatcgttgtg attggtggag
    10741 gtcctgctgg ttatgttgca gccattaaag ctgcccaact cggcggtaag gttgcccttg
    10801 ttgagaaatc tgaacttggt ggaacctgct taaaccgtgg atgtattcca accaagacct
    10861 accttcataa cgctgaaatt attgaaaata tcggtcatgc tgcaaatcgt ggtatcgtga
    10921 ttgaaaatcc taacttcact gttgatatgg aaaaactttt agaaactaaa tctaaagttg
    10981 ttaatactct tgttggtggt gttgcaggac ttcttcgtag ctacggagtt actgttcata
    11041 aaggaattgg tacaatcact aaagacaaga acgtcttggt aaatggttct gaattgcttg
    11101 aaaccaagaa aatcattctt gctggtggtt caaaagtcag caagatcaac gtccctggta
    11161 tgcaatctcc acttgtcatg actagtgatg acattcttga aatgaacgaa gtgccagaaa
    11221 gccttgttat catcggtggt ggagttgtcg gtatcgaact cggtcaggcc ttcatgacat
    11281 ttggttcaaa agtgactgtt atcgaaatga tggaccgtat agttccagct atggatgcgg
    11341 aagtttctaa gaaccttcgc ttgatccttg agcgtaaagg aatgaccatc ttgactggta
    11401 ctaaactgca agaaatcatt gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag
    11461 acgatatcat cgcaagcaaa gctcttcttt caattggtcg tatgccagac cttgaaggta
    11521 ttggagaggt tgagtttgaa ttggatcgtg gttgtatcaa ggtcaacgaa tacatggaaa
    11581 cttcagttcc aggcatttat gcaccaggtg acatcaacgg tactaagatg ttggctcacg
    11641 cagctttccg catgggtgaa gtttccgctg aaaatgccct taaaggaaat catgcagttg
    11701 ccaaattgaa tttgactcct gcagccatct acactctccc tgaagtagca gcagtaggtt
    11761 tgacagaaga acaagcccgt gagaaatacg atgttgccat cggtaagttt aactttgctg
    11821 ctaacggtcg tgctattgca tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata
    11881 agaaatacgg agaaatcatt ggtgtacaca ttattggtcc tgcagccgca gaattaataa
    11941 acgaagcatc aagcatcatc gaaatggaaa tcactgttga ggaaatgctg aagaccatcc
    12001 acggacaccc aacttactct gaagtgatgt atgaagcgtt tgcagatgtt ctaggaatgg
    12061 ccatccattc acctaagaaa aaataaaacg aagatagatt agactggaag gttatttcca
    12121 gtctctatca tataaagtaa agggatatca tgaaatacat tatcaatcat tcaaatgaca
    12181 ctgcttttaa tattgccttg gaagaatacg cctttaaaca ccttttggat gaggatcaaa
    12241 tcttcctact ttggattaac aagccatcta tcattgttgg tcgtcaccag aacactatcg
    12301 aagaaatcaa ccgtgattat gttcgcgaaa atggtattga ggtagttcgc cgtattagtg
    12361 gtggtggagc tgtttaccac gatttaaata acctcaacta cacgatcatc tccaaagaag
    12421 atgaaaataa ggcatttgac ttcaagagct tctcaactcc ggttatcaat accctggctc
    12481 aactcggggt taaagctgag ttcacaggtc gtaatgacct tgagattgat ggtaagaagt
    12541 tctgtggcaa tgcccaagcc tatatcaacg gccgtatcat gcaccacggt tgcttgctct
    12601 ttgacgttga tttgtcagtc ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat
    12661 caaaaggtgt gaaatccgtc cgtgcccgtg taaccaatat tatcaatgaa ttaccaaaaa
    12721 aaatcacagt cgaaaaattc cgtgatttac tcttggaata catgaaaaaa gagtacccag
    12781 agatgactga atacgtcttt tcagaagaag aattggccga aatcaatcac atcaaggata
    12841 ctaagtttgg aacttgggac tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg
    12901 gaaccaaatt cactagtggt aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc
    12961 aagacatcaa gatttatggt gacttctttg gtattgaaga cgttgctgca gtagaagatg
    13021 tccttcgtgg ggtgaaatac gaacgcgaag atgttcttaa ggcgctaaaa accattgata
    13081 tcacacgcta cttcgctggt attagccgtg aagaaatcgc tgaagcggta gttggataaa
    13141 tcaaaaaaag aagttggcca acttcttttt ttgatattta tttacataat ttaaaatacg
    13201 tgaaatcata aactatctag agcattcttt tgttcatcat taacaatatg ggtatagagg
    13261 tcagtgactt gtgtgctggc atgtcctagt tggtgactga ctaaaacttg tgatttagtc
    13321 gcatcataga gcctagttgc tagcgtatgg cgcagtttat ggggtgttac acgtacttta
    13381 aaatcctctg agtatttagc aaccattttc tcaacgctag aagcatcgat acgattagga
    13441 acacctctgt agagagttaa aaaaagggct gtatctgttt tttccgtttt atagcgttga
    13501 ttccgaatgg ccagataatt ctctaaataa ggtttagcaa aagcagcgac attgactgag
    13561 tcacgtttgc caccttttcg agtaacatca ataaccatca tttttagatt gagatctctt
    13621 agatctagat taacagcttc agataagcga acaccagatg ccaagagaag ggcaataatg
    13681 gctaaatctc gttctttatt tttgttgaat gatgagagag ctcgatttga aagctgttgt
    13741 gggtgctctt gatcgatata agttagaaaa ccttctgttt catcacctag aaagagtttt
    13801 tgcttgatat tttcagctct ggcagcaagg gtttctttct ttttcttggt tgaaactttt
    13861 ttcattacat tacgatagaa ataaggttcc ccctgatcgt tttcaacctc ctcggttaga
    13921 tacttgtaaa gactagaaag tgctgataag gttcgattga tagttgtctg tgaaacacct
    13981 tgttttgttg tattagcatt cagcaaggga cgttcacgta gataaaggat aaaggattcc
    14041 atgtctttct tagacatatt ttccaaaact gataaaggaa tatcggatat tttatcagcg
    14101 tttgaaatac cagactccaa aacccagctg aaaaatcggt catattcctt aaggtattcg
    14161 tacagggttg taaaactgta gggcacagcc agcttagatt ggtagtattc cagaacatac
    14221 cagggcatga gttgttttag tttgtcgatt cgttccagta aaatctcacg tttcatgtat
    14281 tttctccata agtaagtata ctagtagtat agcatagact tgtatatttt tcaagaaaat
    14341 tatagttttt cagaaagatg ttataggagt ttttcaaagt gataaaaaga cttagaccta
    14401 caaacaaaac agaataagga aaataataaa aatctcaccc actaaacttc tgttaatact
    14461 acataactga agagctatat aaggagctat gagatacaaa acacattgta taatagattg
    14521 aaactagaat agtacatctg gacttctaaa acattgctag aaattgattt gattgttctg
    14581 attgttttat cctatttcta tttcatttca ctatataatt gctattgttt tctttttcat
    14641 gattttaatc ttactgtaca gatttgatac agtaaactcc tttctagctt tatactttat
    14701 catttctaac ttaattatag tcttattttt aataaagttc aattatttat atgtaaaatt
    14761 cctcatcagt aaaaaataac cgatctcatt tctgagaatc ggttttctaa ataaatcaag
    14821 ctgagtattt acataacata aattatgtaa atactcatac tacaacaaca aatctttgac
    14881 ttttccaatt tcactttttg gaataaccag gtgaatcata tcacccagat acattctggt
    14941 tgagccgtta actgtttggc tcttgccatt atggacttga gttgtgatga ggacgttgtg
    15001 tggtaagttg agttcatgaa cttgtttccc agcaatttta tcagaaactg gtatttcgat
    15061 aagtgtaact tctccttcgc tagatacttc ttctggaagc attttttcca gcatggcttc
    15121 atagactggc gtacctttga gcaaatccat gataatataa gaaacaagag tgacaagacc
    15181 aagtggcatg aggttgcgaa tatctcctac catctcagtt acgaggatca tagcggttaa
    15241 gggagctttt gatatggctc caaaatagcc actcattcct agaatgacaa atatagggaa
    15301 ttgctcttga ctgacaagtc caagattgac acagataaca ccaactaagg aaccaagcaa
    15361 agaaccaaga gctaaaatgg ggaggaaaat tcctcctggc agtccacttc catagctaat
    15421 catactccaa ataaagcgga tcaaaaagta agctaataaa acttggaaac taaaattttg
    15481 ttcagttaaa gaaaggacaa gctgatttcc gccaccaatg atttgaggta agaagattcc
    15541 gactggtatg ataatgataa aagccaagat gggataataa gccctatcca aatggatttt
    15601 ttgaccaatc aagtcataaa ctcttccaac gtttaataca gctttctcat agagaaaacc
    15661 tgaaaatcca aggaaaattc ccatgacgag atatatccaa tactggtcta gggtcatggg
    15721 aggaatgtta tctggcatat ccaatactgg tgtcaaaccg aacatgagta gagacacaaa
    15781 gtttgctacg atgctggctg ctagagttga gacccagaaa aagcgcgaaa aatggtgata
    15841 gacttcttct acaacaaaga gaagtgctgc aataggagca ttaaaggctg cggctaaacc
    15901 tgctgcagct ccactggcaa tcaaggaacg ttcctctact ggactggatt tgagccactt
    15961 ggcaattcct ttaccaccaa ctgctccaag ttgaatgctg ggtccctctc gacccagcat
    16021 gagtccactg gcaatagcaa gaatacctag cacatatttt ttccaaagaa tgccccacca
    16081 gttgagggac atgaggcctt tcagttcggc ttcgacttga ggaattcctg agcctttaat
    16141 atctttttct gaccgtgtta gtttggcact gagccaacag atgagtatat aaaacaaaac
    16201 cagtacaaaa agattgcgca ctaagtaccc ttgatcttga taaactcctt gtatcaggtg
    16261 gaagcccttt tcaattaaga aacgaaagga tccgacgata attccaacga tgaggccgac
    16321 aatgattcct cgaccaactt gggatagtat agtgctggat gcaaaggcga attctttctt
    16381 agaacggact atttccgact gttcctccat ggtatttcct ttcaattaac tttctttttt
    16441 acctaaaacc agtgatttaa cgggttcaaa gctggttcgg tgaattgggg taactcctag
    16501 ttttgtgagg ccttccagat gtttagctgt gccatatcct gcattagtag cgaaatcata
    16561 gccagggaac tgctgatcgt attccttcat caattcatca cgtgttacct tggctactat
    16621 agatgctgct gcgatagaga gggagttggc atctcctttg ataatggagg tttgtgaaat
    16681 gggcaagtcc agtttcatgg catcaatcaa aaggtgctct ggttgagggc tgagctggga
    16741 gattgcttct tgcatggcta gtttggttgc ttcatagata ttgacttggt cgatgacctg
    16801 attatctatg ataccaattc caatcgacaa ggcttggtct tgaacgactt ggaaaatctc
    16861 cagatgtttc tttttaggaa ttttcttgct gtcgttgaga cctttaatct tacaattttt
    16921 aggtaaaata acggccgcag cgactacagg accagcaaga ggaccacgac caacctcatc
    16981 aatacctgca attaaggtca atccttgctt ataaagttct ttttcataag aaagcatgga
    17041 ttccaagcgc aaattttcat ctaattcagc ttgaatggct cttttatgct tgctgatttc
    17101 cttttgaact cctgagcgat tatccttttc aagatctaaa aaaatagggc tttctaactc
    17161 cttgactgtc acaaggagtt ctttgatttc tttaatcgtt gccatcgagg ccttccaatg
    17221 tatctaaggt atagttaccg agtttgccat cacggacttc cttcacgaag agactgtaaa
    17281 aacggtcata gtcatcacgg aaaccgaggg cgcgggtcat atccataata atcacaggcg
    17341 cttcttcttc aattttcatt tgtttgaagc gttcagccag cttttctgga taatgttctt
    17401 tgaaataatt gataccaaaa atggtaacct catccatagg aagcaactga tccttgatag
    17461 ctccagtcaa tgccaactta agtgcaacag tttcatcctc aaacttaggc cagagaatcc
    17521 ccggtgtatc caagatttcc agatctttat tggttttaag ccattgttga ccttttgtga
    17581 cccctggctt gtttccaaca acagcaatct ttttaccagc caaacggttc atcagagttg
    17641 atttaccagc gtttggaatc ccaataatca tggtacgcaa ggtttcaatc tgaatcccac
    17701 gttctttctg gcgagcaatc ttatcagcca tgagcttctt ggccgcatct gttacaactt
    17761 ttacagtcac ttgctctttg gagttgatag ctagcgtctg gattccttgt gattcaaaat
    17821 actgacgcca ttccttggtc attgctggat cagccaagtc ggccttgttt aaaatcaaga
    17881 gttttggttt atcaccaaca atcttggtta acataggatt ttgactagat agaggtaagc
    17941 gtgcatcaac taaaatcgtc acaaaatcaa caaattttaa attctcctgc acctgtcgac
    18001 gagctttaga catgtgacca ggaaaccatt gaatagtagc cattgagttt tttcctttca
    18061 tagcaaggat ttagatcccc tattttattt tactattgtt tgaacataga gagttttcaa
    18121 atgaatgatg tccaaacagt tcttgtttta tgacatagaa atctacttta tattatatca
    18181 tgccttcttt attttgtccg atgatagcct tttaccctat atagaaaaaa gccatcaaat
    18241 gatgactttt cagagggagc aaaaaactgt tcccgtcata ttatgaacca aaagaactaa
    18301 actaccaaca gctaataggc ctaaagctgt aagtgcagaa gttgatgaac ctgttgcagg
    18361 aagagtttcc tgctgataaa taagagcata acttacttca ttatcagtag gaattcctgc
    18421 ttgaatcttg gtttgttctt catcaccttt ttaaacttta tttcctaata gtaaacacta
    18481 ttttttattc tcaaaaattg aacttctaaa atcatttgtt tggtcaagat aggctttaaa
    18541 gattgctctc ttcaacttat gaacggcact gtcgatttct ggatcataat tactccaacg
    18601 tggaccttca gcatccttga gaacagcttg gtccatgagg gcttgcaatt catccacatt
    18661 attgatagtc tttgtgccat agcttggcca tggttttgtc ggatctttaa aggtcacctg
    18721 cttcaagttt ccaaactgat ccactcgttc ttggtacata gctgtcttaa attctgccca
    18781 agttttatac ttaccgtcaa ataccttttt caaaacaagc tcatcggtca ccaagccccg
    18841 ttctttacca taaagattga tagtttgccc ttgttgttta gcatcttctt cgtattggtt
    18901 tgagatataa ggtaccattc catccttaaa gcctttggca gccaaaagtt cgtacgcaat
    18961 cctacgtccc ataaggtccc ctggtgtgcc tttctcactg ctgagagctg aatagattgg
    19021 ggcaaagagt ttgatggtat agtagccatt tcgttcatag tcgccagact ggtactcacg
    19081 agctgataag atgttgtggt caatcaaact ttcaaaacta ttcaggtttc gggcctctgc
    19141 ttctgtcaat tctttgacta cgttagtggc ataaacctca tttccatctg ctggatcttt
    19201 cacatacttg ttctcaattt ttctgagagc cgccattttc tggtaaacac ttagtttatt
    19261 aacgattgac tgcccttcga gatattccat catgtagata aggtcaaaca tgttatggac
    19321 atagttctga agatctgctg cgttttggaa tctctctgtc ggatccaaga cttgaagacg
    19381 ggatccctct gtactatctg attttgagtg tttcaagata gaattaatgg taatggttgc
    19441 gtcacttggt tggtcagggg cttgcaataa gccttttgcg aagaactctg gtcccaagcc
    19501 atttcttcga ccatagccac caaggtaaat atcctgatcc gaatcatggg tcatttcatg
    19561 ggtataagta atggctccat ccttgtccag catgcggtaa gacatatagt agacgccgtc
    19621 ccctgtagcg taggctccat gctggttgtg tactacctta ttgccaactg gtccaaagaa
    19681 gttcttcata gctggattgg agctatcaaa cttagcctcc gctgtagctt ttcctgatgt
    19741 tgtgtcatcc ccaaacttat aggcatcata aaggagaatc gtacgataga gtttttcgcg
    19801 tgactgttca tctaaaatac gataccaata atcgtagtga tctcgctgac gtttggctgt
    19861 ttcacgcgca ttttcttcaa caaaatcatt gagagccttg cccgctttat ggtcactact
    19921 gcggtagcga tcataagctc caaatcctag actagacatg gtcgagatga caaatacgga
    19981 tctctctggc aaggtcagga gaggcaagac catattgcgg tatttccatg tggcactcgt
    20041 gatacgatca taaacaccga tagaatactt ggtgccagct aacccttgct tcgttttcac
    20101 ctcttcgata gtggattttt cttcgacaat gtaagcctta gtctctgatt taaaccagtc
    20161 attattgctt gtatttggta aaaagacttt tcggtaatgt tccagcgtgc taaacaaatc
    20221 tgtcgttcca tgttgactgg caagactgat accataagta tcgacattat tcttagctag
    20281 aagattgtta aagccagatt tacctaactc aatcagagta tctagtgggg aagtatttcc
    20341 tttaccaaag aagtctggat gatacataac taggtctttg acattcactt gaccatagtt
    20401 aaagttatac caacgttcta aataagttaa accgagtagc aaggcttcct tattattctt
    20461 gattttatct acaagatagc ctctggtgac tgagttatcg ctagctagac cggcatctgc
    20521 tgacaaaagt tttttcaagc tgtctcccag attttgtttt gttttggaga actggtcttc
    20581 taaatagagc tcggttaact taacttctgg agaaatacca agtgtctttc tgatagcatc
    20641 tgactggtaa tcaagcttct gcaactcagg caagacttcc ttaacaatag agtctcgatc
    20701 atataagaat tggttaggag tgtagagaag tcccgtatta cctagattat attctgctag
    20761 attggcaaaa tcagtctggt acttgagatc aaatttctca gatgaatggt cattatagtg
    20821 aagtaagagt ttgtttgcag tctgtttgtt ggaaacaata tctgtgatta cttggtcatc
    20881 cttcatcata actgcagata agagttcttt ttggtataag agactgttct ccttaaccag
    20941 gtttccgtat ttgacgatgg ttgacttatt ataaaaaggt aacaattttt caatgttctt
    21001 ataaaccaaa gcacggttag cttgataatg ggctatctta gaaaaatcac tgtctttctt
    21061 gccacttgtt gagaggggtt ccaccgttgg tagagtgaga ggatttattt ctgctttttt
    21121 gcttacaatt tgagaagcat ctagcattgt tcctctttct tcaaaggatt ccttgctgac
    21181 gacctcatcc ttctctaaag tgacattgaa aactctatta gccttactac taaatgtatt
    21241 agctaccttc attcctgtat agtggtatcc agtgatggca tttccgtttg ttacattgac
    21301 atcgctaaga acattggtca attcaccagc gtgtttttcc tcaccagaag ttcgatccca
    21361 caaatatcct gccacaccag cgactttacc aaagtgcttg acattattaa tatcaccttc
    21421 cgcataactg ttctgtatat gcgcatcttg gtctactaga cctgcaagac caccgacagt
    21481 ctgatctgac ctattggtgt ttgacgaaat ggttactgtc gctttggatt tagcaataga
    21541 cgcatttttt cctgttaaat gaccgaccag accgccaata ttgtaggcat cagtcgtttc
    21601 ataggtattg acaattcttc ccttgaaact gctctctgcg atgcttgatt ggtctgcctt
    21661 agccaacaaa ccaccgacac cacgttctcc agccagaaca ccatcaacat gaacttgttt
    21721 aatctttgtg ccattcgtag cttcatttgc tagtgaacca atatcatttt tccctgaaat
    21781 agcaacattt tttagactca gtttttctac tgtagcacca ctcaagtttt caaacagagg
    21841 ttttttcaaa ttatagatag cataattctt gccatctttt tcaccgatta aacgaccagt
    21901 aaaggtgtcc ttgatatagg atctttcatc aggaccaagc tccacttcgt tagcattcag
    21961 gctggccgct aaatgatagg ttccagaggg attttggttt atagctttga ccaggttgct
    22021 aaaggaagta aagtttgtat tttcctcttt agccttctta tcgaggtaga aggtgaaatt
    22081 gtcgacatat ttattttcta gttcttgctg gagtttctca gctttggctg tgattttata
    22141 aactgactgc ccatttcttt tctcttcagt tattgaagct actggtatat agacatcttt
    22201 aaatgctgaa gattttacct tgacaaagta agtatccgta ttctctggaa ttccatccaa
    22261 tgaaacatgt tgtctgtagg ttccattcgt ctgactatac aactcaatat cagaaacatt
    22321 tcttaattcg agttttttct ctggttctgt ttgtccattc tctgatgttg tattacctga
    22381 attttctggt tttgttgcag ttccgttttt ttctggttga tttgattctt caactggtgg
    22441 ttttgaatca ctaggtttat tggatacttc tccagtattt tcgttagcta ttttcccaga
    22501 gtttgtttgt gtttcttctg caggttgaac tggtttttct gtttcttgat ttgaggtacc
    22561 ttctactgtg ccttcatttg gatttactgg aacttcttct acagtttttt ctgaattttc
    22621 atttttagag tcattatgtt ctggtttatt tgattctcca actgaggttg tcgaatcact
    22681 aggattactg gacacttccc cagtattttc gctagatgta tctggtgata ctttctctga
    22741 attcgttgtt gattcttctg caggttgaac tggattttct gcttcttgaa ttgaggttcc
    22801 ttctgtagta ccttcatttg gatttactgg tgtttcttct gttggtttta ctggaacttc
    22861 ttcagttttt tctggacctt gttctttggt cttctcaacc ggagtttcag gttttacttg
    22921 ctcaatatta cccttatatt ctggcaatgg ggctacctgc tctggttcac ctttatcact
    22981 taccacagta tctggcgact ctggttgaac ctcagtctca cctttgtcgg tcacaactgc
    23041 ttcgggtaat gtcggttgaa cttctggttc gcctttatca cttactacag cttcgggcaa
    23101 ctcaggctga attgcgggtt caacaataga tccagactgt acgtccttat gttctacacc
    23161 agtctcaggt tgttccttta taacttgagt ttttttagta cctttttcga ctattcttgg
    23221 actaggcgca gtcgttgaag ttgaaacaat ttctcgcgaa acttcttcct tgtttacaga
    23281 gaatattctg acgatttcaa ctttcttacc taatttacct tcttgtttta ctcttacagt
    23341 tccttcagct aaatcaggat tttcttgaat ttcttcttga aaatctattt ttgtctctat
    23401 agtttcctca cgatataaga gttcaggttt gttcaattga cctgataaaa cttcatcctg
    23461 tggatttaat gtatttaccc cagtcttttc ttttggagaa atcttctcct ctttcttcgt
    23521 ttctagattc ttatgttcgg ctaattgttc ttgagaatct gaagattgtt tctcttcttt
    23581 tcttggattg attaattcag tagagaaagg tttttcaact acttgaactt ctgtcggctt
    23641 agttgaagaa acaggtgttt gttcctgaat agcttgtact gttgatggat ggtctacaaa
    23701 atttggtgta acattataat ccaccttttg ttgttttgta ggagtggctg ctgatttttc
    23761 ttgattactt acttcaaaat cagaagtcgt ttttccctct ttgatatatc caatataagt
    23821 gtaacctgaa atctctttag gaagaggtaa tttttctcca gaggtcaatt catagtccgt
    23881 attgtaattt agcaaaagat gattttctaa agcatggact gaaactaaga caccatttcc
    23941 cattcccgca accaatacta aatgtaatac cgttttattc ttaacctttt tcttggaaac
    24001 agcaaaaatt aaaattccca gagcagctaa gctagcacca gcaactaggg cttgcctctc
    24061 attcttgctt ccagtatttg gtaattccgc cagttgattt tgagaattta acttataaac
    24121 aagataataa gtttcatcat cattctccac gtatgtcgga atatcataga caagctgctt
    24181 cttttcttct gatgatagct ctgaatctgc cacatattta tagtgaactc ccgcagtttc
    24241 ttgagcatcc acagatgaac tagctaatac agacataaaa aataaacttg aaatcgttgc
    24301 agatacaagt cctactgata attttctaaa tgaaaaacgc tcttgttttt caccaaaata
    24361 cttttccatt attcctcctt gaaataaaat ttatatatgt tacaaagacc tttattatat
    24421 tagtgtatta tctattatct atagaaaagg cagtatacct taattatact cttaatttac
    24481 aaaaaagtct taaaattgag atgcgctttc atactttgtt ttatattatt tgggggtaca
    24541 ataacaccta ccatgaaatt tacacggtag gtgttactca tatcactaat cgttctaaaa
    24601 atggtttgag gcagttgagg agaattcctt ctatccagct tccttgtgct gatgagcgat
    24661 ggtcttcctg caggcttttt tttagaaaat ctcggacttg ttctggtgcg atttcaaatt
    24721 caaaggcttt cattttatag aaaaagtcga tgagatgatc tgacaggtat tcagttgaaa
    24781 agggtacttc accacttttt cgatattcta ataagagtct agaaaatcga gctttttctt
    24841 caggaagctc acgaaaatag gaattgagga tccaagtctg cttctgtttt ctttcaattg
    24901 gatcctgact ggcaattcgt tggtcttttt ccagctcttt ttggtattgt ttggccttga
    24961 tagctcgttc tgctctattt ttaccaaaaa gaattttttc ccacttgcgt tcttcttgag
    25021 tcagggtctc tgtaaagcca aagtaatctt gataagcacg ctctgcgggt cccatggcta
    25081 gaaccagatt gtctgcatat tgcttggcga ttttatccct cttcttgcgt tctttctctg
    25141 cctggatacg gagttcttgt tcgtagtcaa ttttctcctt gcctagcttg acaaggtaga
    25201 gttggtcatc cgatttccca agtaaaaagg gtttgataca cttttcaagg acttcttcca
    25261 tccgagcctt tttctttggt tccgccttgg tccaacttcc tccctgaaag acttctagga
    25321 aaagctggta gtctctctca ggcgcaaatt gattgccacg attgggtttg aaaacacctt
    25381 tttcccagag ccattttaga agtcgctcgt caaagtcact tttattgacc ttgatttttt
    25441 cctttttctg agcttttctg gttagatttt caacctttct gagcagtttt tcttcctctt
    25501 ccaattgctg gtcaaggggc aatcgatgaa aatgacgaac acagtcgcta ccaattggaa
    25561 agaggcgttg gcctgtgaca ccgttaaaga gttcataagc gtatttgatg gcatttcgac
    25621 agacacaatt gctacggcca ataccgttaa aaatcaagga aacttcattc cattccttgg
    25681 tagcttgttc ccaagtatcc gctttcgaag cctgtaaaac tgcatcgtgc agggattttc
    25741 taactggaag tgtcatgagg tctcctttct aatactcaat aaaaatcaaa gagcaaacta
    25801 gaaagctagc cgcagtcagc tcaaaacact gttttgaggt tgtagataga actgacgaag
    25861 tcagtaacca tatatacagc aaggcgaagc tgacgtggtt tgaagagatt ttcgaagagt
    25921 ataagttata cttttacaac ttgaacctcg tctttaccga gtaaaatcaa gtatttttca
    25981 atattttcaa tcgaataggc tcgtgataaa gcctcttcgt atagagctaa ctgaccacga
    26041 tagcggtcta cgagttgact tggttcatca tagcggtctg tcttgtagtc gaacagaaca
    26101 attttgtttt cgtaaagcag atagccatca aggataccac ggacaacaaa gtcttcctga
    26161 ctcttttggt ctcgtttgag catggagaaa ggttgctcgc gatagagatg gtcggtatta
    26221 gcaagaattt cctgaccgag tgctgtgtca aagaaagcaa gaattttatc aagattgatc
    26281 ttgtctctga cagcttggct agtttgaact tgtttgagtg tttctgttaa gctagcaagg
    26341 gttagttgct ggctgaggtc aattatctgc atgagttcgt gagtagcact accaatctca
    26401 gctccagtta ccttttcttt ggttgaaaaa tctggcaaat cgaagctgat tttcttgcct
    26461 actgactgac cttgaccagc aatctcgaca ccttccatat ccataactgg ttcgtagaat
    26521 ttcttgattt gacttggggt ttgaacacta ggaagttcaa tagctgcgcg gtgaagagta
    26581 ttataaactt ccacctcctt cagcatttcc agagcttctt tgatggtatc tgactgacga
    26641 ttgtctgctt gggagctatc ttggagagga ctcttggttt ccaactctcc gatagcttct
    26701 ctggtcaact gatcttcgtc aataaaacga taactaaagt tgagcttgtc cttagtaaac
    26761 actttactga tagcccaaag ccaatcttgg aaattccgtg cttgcagtct agtattgcta
    26821 tttagtttcc catttttggc tgctgggtat tccttggatt ccagcttttc acgagaaccc
    26881 ttgccgacaa gatagagctt tttctcagcc cgcgtcatag caacatacag caaacgcatc
    26941 tgctcagaat agcttgctag ctgtaattcc tcttcgttct gcctataggt cagactagga
    27001 atggagagtt tgatggtttt aggatagtgg tcttctactg cccctgtctc catcttggca
    27061 atatatttga caccaagacc attctgacga ctgagaatga cttctgacat agagtcttgc
    27121 ttgttgaaat cttgatccat attgaggata aagacgtaag gaaactccag ccctttactc
    27181 ttgtggatgg tcatgagctc tactgcatct tttggcggtg cgacggccac gcttgccaaa
    27241 tcgtgctggg cttctaagac ttggtcaatc atacgaataa aacgcgacaa acctttgaaa
    27301 ttgctctttt caaattgatc agcacgcagt gctagggcat agagattggc ctgcctagca
    27361 ggaccattcg gcaaagcccc aacatagtca taataaaaac ggtcgttgta aatcttccaa
    27421 atcaagtcat agagagagtg ggttttggca tacaagcgcc aagaagctag gatatccatg
    27481 aattgcttta gtttttcaac tagagctgtg tgaatcaagc ctttttgact acttgccatt
    27541 ttttgtgcat tgaccagttt ctcatagaga ttttcgtgga ctttatcctc tgctttctga
    27601 agggacaaac gtgctagctc atcctcatca aaaccaaaca ttggagactt cataagggca
    27661 accaaggcgt agtcttgcag gggattgtga atgacacgaa gagtgtctag catgacttgc
    27721 acttctaggg attggagata attgttttga tctccgtcag ttttgacagg aatcccgtac
    27781 tcagacaggg cgaggagaat ctggtcatta cgactgcggc tggaggtcag aagggcaatt
    27841 tccttaaagg caacaccttt ttcttgatga agtttcagaa tctccttgat aactaagcgc
    27901 atttcgcctg ttagtttcgt ttctgtttga ctctcttctt cctcacctgt atcgtccttg
    27961 tcgtagagga gaaattctgc cttgttgtct ggattgggag tcagtttggt attggcaaaa
    28021 acaagctggt gcttgttatc atagttgatt tcaccgacct cttggtccat gagacgttca
    28081 aagacatcat tggttgctga cagcacttct gaactactac ggaaattttc cttgaggaga
    28141 atgagcttgc cttcttgggg attttgcgca tagcgttgga atttctcatt gaaaatctgc
    28201 gggtctgcct gacggaaacg atagatggat tgcttgatat ctcccaccat aaagcgattg
    28261 tggccattag acaacaattc cagcatccgt tcttgaatat ggttggtatc ctgatactca
    28321 tcgaccatga cttcatggaa gcgctcctga taagactcac gaacttgtgg gaaattctct
    28381 aaaatctcaa tggtgtaatg gctgatatca gcgaattcaa aggcattttc ctggcgtttg
    28441 cgctgacgat aagcctccac aaaatgactc ataaaggttt ggaaggtttt agctagtttc
    28501 catgtgtctt gatgataacg ttcttgatag tcgagaatgg ttatctggtc tgataattgt
    28561 cctagtttag caaactgggt ctttctctct tcgttgtagg catcagccag gggcttcaaa
    28621 tcagccttac gactggcatt agtcagagct cgaccgtttt tctccttaga gatggcgaca
    28681 acacgcgcaa gcactgcctg ataagcctga ctatcggact cctgatttag ggagcaaatt
    28741 tcatccagaa ttaactgaac attttctaaa taggcagcct ttgcaaactc cttggcatcg
    28801 ttatccagat ggtaacggaa aaagctttcc aaatcccaaa gggcttgttt gatttgctca
    28861 gtcagttttt ctttttcact ggtaaaatca gctttctcaa atcctttgag gaaagattca
    28921 ctcagccact tttgaggatt actggtggat tggaggaagt catagatttt atagacctgc
    28981 tggcgcagac cccgttcgtc cttgccacgc ccagcaaagt ttttcagcaa atgactaaag
    29041 gtctctttct gtttaccttg gtaatgcgct tcaaagacct catgaaagac ttcgttttcg
    29101 agaagaagtt gctcgctttg gttttgtaaa atacggaaat taggtgcaat atcaagcaga
    29161 taaccatgtt tgccaaggaa tttttgtgtg aaagagtcca tggttccgat ggcagcgttt
    29221 ggtaggtctg ccaactggcg tcccaagtgt tgtttgaggt cgacatcatc tgtttcttgg
    29281 attttcttgc tgattttttt ctctaaacgt tctttaagtt cagttgcagc cttgacggta
    29341 aaggttgaga taaagagttg agaaatttcg acaccacgcg ccaattggtc cagaatgcgc
    29401 tctgccatga caaaggtctt tccagaacca gccgatgctg agaccaggat attctgacca
    29461 gaagtgtaga tagcttcgat ttgctcggca gttttctttt gttccttgct cgaatttgct
    29521 tctgcttctt gcagtttttg aatctcctcc tcacttaaaa agggaataag cttcatcgat
    29581 tcaactcctc tcttattttt tcaagccaag cttgcttgag tttttctccg accagacgct
    29641 tgccatcagc taggtccaac ttttctagga aacgggcttg gcccagatgg taattggctt
    29701 caaagcctgt aatagcctga tgttgctgga cgtatggggc aatgcttctg ccattttcag
    29761 tataaggatt gatggcgaac cggcctgcta aaatcttctc agcagctttc ttgtaaagat
    29821 aggcattgta gtccagtagg agctgaaatt cctcatctgt cagttgattc gccttgtttt
    29881 tgttataaaa ttcgcctaaa taactgcttt ctttttccaa gaagagccct tggtatttca
    29941 tagatttgct ggcttctacc actgctcctg ccagactttt taccgccatc agagattgga
    30001 caggttcagc catttccaag tacatggcgc cgaaaaagtt ctgctcccct tctcttttta
    30061 gggcagcaag ataggttggt aactgagaat tgagcccatt aaagaaatga ggaaactgga
    30121 actgagtcag actggatttg tagtctacta ctcctatcgc tccattagct ttcaaacggt
    30181 caatccggtc caccttgcct cgtacaaaga cactgcgtcc attgtctaat tgaataaagg
    30241 cttggtcttt tccaccaaaa tttgcttctt ctttgattgt ttcgatggct ggattgtgtc
    30301 ggagaatatg tccagttgtc cgtgcaacat caagcaaaac ttccttggta aactgggctt
    30361 ccaaactttc ttgataaata gcttcaaatt cgcgttcttg actggtttct tgaatagctt
    30421 gttctagacg ttggtcaaag gaatcttcat taggcaactg caaggcgcgt tcaaagatac
    30481 gatgcaagaa attcccgtga ctacgggcat caggacgcaa acgtaattcc tcctgcaagc
    30541 ctaaaacgta gcgtaggaaa taactgtatt cattgcgata aaactctgtc aaacccgacg
    30601 tagacaggta aaactcctgt ttggcaggat agagagcttg caaggtgtcc ttggctaagg
    30661 tcttgctgct tggactgatt gggatggctg gattttccag accttgctga tctagttttt
    30721 tacctatgac acgcgacaga accttgacaa aagtcaaatc ttgctcagta tcgctcatct
    30781 caccctgctg gtgataggca accagactag acaaaagact gtgataggac cccatatcct
    30841 ccttagacag tcctttgtga ttcatcctct tctctctccg cctaaatcca aaatggatca
    30901 actcttgaag ataggcagat tccttacttt cactttcgtt aaaaaggctt ggagccgaca
    30961 agaacaactg cttacgagca gaattgacca aggaaagcat agtgtagcga tttttcttga
    31021 gattttcact gctggcaatc agtaattgaa cgccttcttc ggtcgtttgg tttaggtttt
    31081 gcctttcttc atctgtcaga agactggtgt tttgagaaat ttttggtaaa ttgtcctgag
    31141 ttagtccaat agcatagaca aagtcagcag tcaatggtgc aatcaaatcg taactctgca
    31201 ccagaacagt gtccactgtt gctggaatgg tacggtattg ggacaaactc attccagaat
    31261 ggagcaaggc taggaagtct tccagactaa cctgtgaacc agcaaaaaca gtcgcaaatt
    31321 gttctaaaac atggcagaaa gccttccaaa cttcggcttg tctttcctgt tctacagctt
    31381 ccaaagtggt tgtcaaatct tgtaactgct tggtcacagc tccttctttt agaaagacac
    31441 tccatttttg taggaggttt tcagcctttt gttttcggct ggcaaagagg gtttcaagag
    31501 gtgctaaaat tctcaggcgg aggacattca aacgctcaag attaaatttt ccatggtggg
    31561 atttggtgaa agtttgctga aaggctggca agccattgat accaagatag cggatatatt
    31621 gctcaaaagc atcaatatca gactgactga ggtcagtata caaatcagtt ctaagaagat
    31681 taatcaaatc ctcctgacga aaacggtaac gttttaaagc taaaatagac tcgacaaact
    31741 gagtcaaggg atgatgagcc atggcttcgc ttctaccaag ataaaaagga atctgatact
    31801 ggtcaaaaat ggttttgaga gataactggt aagaagctac atcccccaag agaatacgaa
    31861 aatgcttgta gctcaggtct gagttctcat gtaatttctg acgaatgcta cgggctacta
    31921 gctccaactc ctccttttgc gtcaaacaag accagatttg taaattttca cggtctttct
    31981 catcgacatc caaagcgagt tctgaaaagt cataagaaga ctccaacaaa cgagaggcct
    32041 tgtcaaaact atccatcttc tcatgagttt gagaacagtc ctgagcaggc gtttggtatt
    32101 tagaagccag atgatggaga aattttacgc tggcttggta gagattgccc tcgctaaaag
    32161 gactggtata ggctttctta ctagcataag ccccgataac aatctcaaca cctttgccgt
    32221 gaagtaagtc cacaacccgc tcttcctcag cagaaaaacg agtaaagccg tcaatgacca
    32281 aggtgatttg attaaaatca ctacttacct tgtcattctc aatagcctca atcaaatggg
    32341 acaactgact tccctgggct aactgacctt gattaagata ggctgttact ttctcaaaaa
    32401 tcaagagtaa atccgccctc ttatcctcat ctgttaaatt ctccaagtcc aaaaaactca
    32461 tctgagattt ggtcatctca tggtaaagct caattaactg ctggatcaat tgaggatcct
    32521 gcttaatagc gccataaaca cgcaagtcct tgggatcgag ttcggcaagg catttgtaaa
    32581 aggccaaccc aagaccgata tcatcaagag tagttttaac tggtaaatca ttcaagacca
    32641 gataacgagc catttgagca aagcgcgtga cggtaatcga aaaagaagcc tgctgggaca
    32701 agcattccag cacggcgcgt tccttttcaa aagaaagaga gttgggggca atgtagaaga
    32761 cccgcttgcc agctgcaact agctcttctg cctctcttgt tagaatttct gtcaaagaag
    32821 ttcgaatatc agtataaagt aatttcatct cagcctcgtt ggaatttttc atcaccctat
    32881 attataccat gattagcctc gtaaatctgt taaaatattt aggccatcct ttcttttctt
    32941 catcatctgc taaatcttaa atacttagct ttacttgtat tagatagaat aagtctggct
    33001 actgaaaatc acataataaa aaagcctcgg taacaaggct ttgagtttta tgattgtttc
    33061 ttaggtacgg aatacacttc aatgtgttgt cccagtatct taatgtcgac tggtagattg
    33121 tctgatttat cgccatcaac atcggactct aattcgatat cagaagaagt tttaatatta
    33181 cgtgccttta tatattcaat attcttgata gaatgattga actatagtaa attgaaacta
    33241 taatagtaca ccgtggatgc taaaatattt ctagaaatta atttgatttc cctaatcaag
    33301 ctgttcgtat cttatttcaa tcta
//
LOCUS       DAVBCV010000019        32805 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBCV010000019 DAVBCV010000000
VERSION     DAVBCV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.019"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(331..1179)
                     /gene="dprA"
                     /locus_tag="VQ516_000864"
     CDS             complement(331..1179)
                     /gene="dprA"
                     /locus_tag="VQ516_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2511331.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1277..1966)
                     /locus_tag="VQ516_000865"
     CDS             complement(1277..1966)
                     /locus_tag="VQ516_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2511332.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQNIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     HKLEEILKNEN"
     gene            complement(1978..2856)
                     /locus_tag="VQ516_000866"
     CDS             complement(1978..2856)
                     /locus_tag="VQ516_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2511333.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2846..3715)
                     /gene="licA"
                     /locus_tag="VQ516_000867"
     CDS             complement(2846..3715)
                     /gene="licA"
                     /locus_tag="VQ516_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2511334.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3732..4754)
                     /locus_tag="VQ516_000868"
     CDS             complement(3732..4754)
                     /locus_tag="VQ516_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2511335.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4759..5466)
                     /locus_tag="VQ516_000869"
     CDS             complement(4759..5466)
                     /locus_tag="VQ516_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2511336.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5802..7289
                     /gene="tacF"
                     /locus_tag="VQ516_000870"
     CDS             5802..7289
                     /gene="tacF"
                     /locus_tag="VQ516_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2511337.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGDILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7299..8102
                     /locus_tag="VQ516_000871"
     CDS             7299..8102
                     /locus_tag="VQ516_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2511338.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRLVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSATFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8104..8913
                     /locus_tag="VQ516_000872"
     CDS             8104..8913
                     /locus_tag="VQ516_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2511339.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDQFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(9188..12364)
                     /gene="carB"
                     /locus_tag="VQ516_000873"
     CDS             complement(9188..12364)
                     /gene="carB"
                     /locus_tag="VQ516_000873"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2511340.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(12676..13755)
                     /gene="carA"
                     /locus_tag="VQ516_000874"
     CDS             complement(12676..13755)
                     /gene="carA"
                     /locus_tag="VQ516_000874"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2511341.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(13805..14728)
                     /locus_tag="VQ516_000875"
     CDS             complement(13805..14728)
                     /locus_tag="VQ516_000875"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2511342.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(14747..15268)
                     /gene="pyrR"
                     /locus_tag="VQ516_000876"
     CDS             complement(14747..15268)
                     /gene="pyrR"
                     /locus_tag="VQ516_000876"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2511343.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(15479..16108)
                     /gene="nth"
                     /locus_tag="VQ516_000877"
     CDS             complement(15479..16108)
                     /gene="nth"
                     /locus_tag="VQ516_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2511344.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(16108..16650)
                     /locus_tag="VQ516_000878"
     CDS             complement(16108..16650)
                     /locus_tag="VQ516_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2511345.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            16829..17146
                     /locus_tag="VQ516_000879"
     CDS             16829..17146
                     /locus_tag="VQ516_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2511346.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNDQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            17385..18944
                     /locus_tag="VQ516_000880"
     CDS             17385..18944
                     /locus_tag="VQ516_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2511347.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(18988..19887)
                     /gene="htpX"
                     /locus_tag="VQ516_000881"
     CDS             complement(18988..19887)
                     /gene="htpX"
                     /locus_tag="VQ516_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2511348.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(19889..20449)
                     /locus_tag="VQ516_000882"
     CDS             complement(19889..20449)
                     /locus_tag="VQ516_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2511349.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            20543..21256
                     /gene="rsmG"
                     /locus_tag="VQ516_000883"
     CDS             20543..21256
                     /gene="rsmG"
                     /locus_tag="VQ516_000883"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2511350.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            21559..22842
                     /locus_tag="VQ516_000884"
     CDS             21559..22842
                     /locus_tag="VQ516_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2511351.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(23037..24608)
                     /gene="ffh"
                     /locus_tag="VQ516_000885"
     CDS             complement(23037..24608)
                     /gene="ffh"
                     /locus_tag="VQ516_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2511352.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(24620..24952)
                     /locus_tag="VQ516_000886"
     CDS             complement(24620..24952)
                     /locus_tag="VQ516_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2511353.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(25043..25420)
                     /locus_tag="VQ516_000887"
     CDS             complement(25043..25420)
                     /locus_tag="VQ516_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2511354.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            25492..26796
                     /locus_tag="VQ516_000888"
     CDS             25492..26796
                     /locus_tag="VQ516_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2511355.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTVTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            26809..27615
                     /gene="yidA"
                     /locus_tag="VQ516_000889"
     CDS             26809..27615
                     /gene="yidA"
                     /locus_tag="VQ516_000889"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2511356.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRPMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            27658..28656
                     /locus_tag="VQ516_000890"
     CDS             27658..28656
                     /locus_tag="VQ516_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2511357.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(28823..28894)
                     /locus_tag="VQ516_000891"
     tRNA            complement(28823..28894)
                     /locus_tag="VQ516_000891"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28859..28861),aa:Arg,seq:cct)
     gene            complement(28937..29284)
                     /gene="rplS"
                     /locus_tag="VQ516_000892"
     CDS             complement(28937..29284)
                     /gene="rplS"
                     /locus_tag="VQ516_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2511358.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(29403..29732)
                     /gene="crcB"
                     /locus_tag="VQ516_000893"
     CDS             complement(29403..29732)
                     /gene="crcB"
                     /locus_tag="VQ516_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2511359.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(29726..30100)
                     /gene="crcB"
                     /locus_tag="VQ516_000894"
     CDS             complement(29726..30100)
                     /gene="crcB"
                     /locus_tag="VQ516_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2511360.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(30097..30363)
                     /locus_tag="VQ516_000895"
     CDS             complement(30097..30363)
                     /locus_tag="VQ516_000895"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2511361.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSYILKRSRDYQDQNIK"
     gene            complement(30479..30922)
                     /locus_tag="VQ516_000896"
     CDS             complement(30479..30922)
                     /locus_tag="VQ516_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2511362.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            31026..31961
                     /locus_tag="VQ516_000897"
     CDS             31026..31961
                     /locus_tag="VQ516_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2511363.1"
                     /translation="MEICQQILEKIKEYNTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            32015..32299
                     /locus_tag="VQ516_000898"
     CDS             32015..32299
                     /locus_tag="VQ516_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2511364.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(32356..32574)
                     /locus_tag="VQ516_000899"
     CDS             complement(32356..32574)
                     /locus_tag="VQ516_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511365.1"
                     /translation="MSISPRFEALEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(32571..32805)
                     /locus_tag="VQ516_000900"
                     /pseudo
     CDS             complement(32571..32805)
                     /locus_tag="VQ516_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         9950 a         7070 c         5988 g         9797 t
ORIGIN      
        1 tcactatact ttcccttata ctcaatgaaa atcaaagcgc aaactaggaa gctagccaca
       61 ggttgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag ctcaaaatac
      121 tgttttgagg ttgcagatgg aagctgacgt agtttgaaga gattttcgaa gagtattata
      181 tgagaaaaat cctctactct actaatccac ttagaaaaaa cgcatattat caagtgttaa
      241 aaacttgcta atatgcgttt tattatggaa atgttgggtt cattttctct taaaattgat
      301 tttttcttag aattctagct taggtcattt ttaaaattca aattccgcaa gaacatcttg
      361 cccactggtg accaattttg ctccttcttg aatcaaatga tggcaaccgt ctgatagtcc
      421 atctaaaatg ctaccaggaa tagcaaagac atcgcgtcct tcttccattg ctcgctcaca
      481 cgtaatgaga ctacctgaac gcatcttagc ctctgctaca atcacaccac gacaaagtcc
      541 agcaatgatg cgattacggg caggaaaatg aaatttcaga ggttgttcgc caggtccata
      601 ttcacttaga accagatggt cattgccgat gtagtcttgc aagcgtttat tggctttagg
      661 ataaaacaca tccagtcctg ttccaatcac tgcaatggtt tttccgccat tctgaagagc
      721 tgccatatga gctgctgtgt caatgccctt ggccagacca ctgacaataa ccagttcatt
      781 ttccaagcct tgaatgactt tttcaactga tttagctccc tgtttgctac aagcacgact
      841 gcccacgacc gctaccttcg ggaatttcag gaggtcaaga tttcccttgt aaaataaaag
      901 tacaggcgca tcatatattt cactcaaatc ccaaggataa cagtcatcta aaatagagaa
      961 agatggaaat ttttgaaact ctttcgacaa atgcgcatcg tctatctgaa aataacgttc
     1021 cataaaaacg gctggattac ggcaacctga tatatctgca atatcaccca acaaaagctc
     1081 ctgatcaaca ttttcaccgt attctagaac tttcaaaatc tgttgattgg tcaaacctga
     1141 tttttttaac ttatagattt catagtttgt gattttcata aataactcca tttctttttc
     1201 tactcatcta tctattcgta aaagttcgta aaaaatcaaa aaaacatccg actattttgt
     1261 cagatgttgg aagcttttaa ttttcgtttt taagaatttc ttctaattta tgatagtctt
     1321 ggacactatc aatctcataa atgctattgc cttctaattc ttcaacatag acatctagct
     1381 ctttgatgtt atccttaacc atattgtccc aatagagatc aacaaattcg ccgcttgcat
     1441 aagccttatc gataaagcta acaatttttt ctgctgttgg agcatcccag aaggatacac
     1501 cactaaggat gcgacctgcc ttgctatcaa caataatgtt ttgaaccttg tagtcatctc
     1561 catagaccaa gaaccattcg ttggtacaat cttcacgata gacactaaaa taagtagaac
     1621 gtgtcaaatc attgcggaac atatttttaa agagatagtt atcggcatcg ataacataac
     1681 tattggctaa ttcttctttt acaagataga gagagtaaaa gttattgtag tcagcgtatt
     1741 tatcattgaa aacgagacga acaccgtatt tctctttcaa gtaatcgaat tgttctttaa
     1801 gataaccaac aatgatgatg atgtcattga ttcctttttc tttgagaaac tcaatttggt
     1861 actcaatcaa aggtttttga ttaacctgaa ccaaggcttt aggggtattt tcagtcatag
     1921 gacgcaagcg agttcccaat cccgctgcta agatgatggc tttcacacga atctccttta
     1981 ttctttaata ataatataaa ctccagcaat gacgacaagt gacgtcataa tggtcagcat
     2041 atctagcggt gcacccaaga aaacaactgc aaacaagacc gtccatacta catagctcac
     2101 gttcaagcct gtagccttgg ctggttgcaa gcgattgata gcgatataat aagccaagta
     2161 ggaaatcata tcaaaggctg caaaaacaat catgagacct agcaattgtc cattggctac
     2221 tgcagtaaat gactgatgag agaagagcac aatcacaaga taggacaaga acgaagttac
     2281 ttgacggatt aagagggctt cgatttcact caattcactt tccatggcaa aagagctaag
     2341 aacactctca cttccccatg caatagcaca aaccaaagca caaagaatcc caatgtagaa
     2401 agaattaacc tgttcaactt tataggtctg ggcaataatc cctccaataa tcaagacaat
     2461 cccaaataca gtatttttcg aaatcttgtg cttcaaaaag aagaaagcca atagaactga
     2521 aatcgcaggg taaatagccg atacagatga agctaaagaa cttccgatat acttaactgc
     2581 ataaagattg gcctgcatac cgatagggcc tgctagcaag gctccgatga taacactgac
     2641 attgcgaata tttaagaaaa ttgagaggcg aactttccct tcttttacca agagaaaagc
     2701 tagtaagata aagatgctca aaaaatcatg agctgcagcc accacaaagg gtgacaaatc
     2761 tgtaaaaatc gaaaagatat aagcactaac cgttagaccc aagccccaga aaatacctga
     2821 gagaaggcca aaaggaactc cgtttttact tttcatctga acctccataa gaagccaaac
     2881 ctttaacagc tctttggtaa cgattcacac catagtcacc aaaatcttca ccttgctctt
     2941 ccttatagac agtccataga ctccaaatag tatcttgtaa aattttataa atagcaatct
     3001 tttcatgaga aaccggtgtt tggtcactct catagtgaga taagaaagtt tcttcctctt
     3061 gggaagtgaa ttcagactct aaaaagaggg cagccaaatc ccacattgga tcattcattg
     3121 atgaatattc ccagtcaatc aaataaagtc gtccttgagg tgattcgata aagttttcag
     3181 gcaccaaatc gatatgacaa gattttctgt caacacctaa gtcagccagt cttttctcta
     3241 aggagaagac tgcatttcta acagattcat agttggcata aggaatttgt tcttcaatca
     3301 aggattcgta ttttttgatt tcttcaaaag gagcaaattc tcctcttaat tccttagcag
     3361 acgtatgaat agtttgtaat attggagcaa ttttgtcgaa cttggtcttg attgacgttg
     3421 aatcaagcgt aatcgcagat tcgatatact catttacttt gataccagct tcaatatcaa
     3481 aaagataatt ttttacatct aagcctaaat cctttagtag ttcaagattg tacttttcat
     3541 cttgtcgatt gataagcttt tctgtccctt taccaaagaa tttaacaatg tattgcttat
     3601 ttgttgtttt ggccaaatag ttttgattgg tcattccacc cagttgttca acactgagga
     3661 cttcctcttc ttgactaagt aaggaagaaa ttttttcttt aatgattttc tccacaatta
     3721 acctccagta cttatacttc ccacttaaac actgttttaa aggctgtgtt taaatcggtt
     3781 gcaaagacac gatgaatatc tttaatttct cttacaggtt cttctagata aaggatattt
     3841 ttaagacgat tggcaaattt cttgacttcc atcatttgga tagcattttt aaaatcaatg
     3901 cgaccagaac gagatgaccc aaccaaaatc aagccctttt ctaaggcatc gcgagtattg
     3961 agattgactt tatattcgct aactcccatc atgagaatcg ttccctgagg acgaatgtag
     4021 cgaatcaagt cattaatagc tggtccagta ccatcaccac cacaacattc aaaagcatgg
     4081 tcaaaggcca actcttcagg aatattatcc gtaatatagc attctttggc aaatgagaag
     4141 agttccaact tttcccaatg acgaccaata accacaatct ctgcttctgg caaagtatag
     4201 ttgataatat tggcaaccac aaaagctaaa cttccatctc caataacggc gatccgctcc
     4261 cgcttgctat gagcaagagt caatagacga ttcatagcgt gcatgcccac actgacaaac
     4321 tctgtaatgg ctgcaaccgt atcttcaata gcatcataag ccaccacacg atctttaggg
     4381 agagaaacaa actctctcat aaagccatca aatccactag acaagaaatg ggtccctgtc
     4441 atgtagtttt catagaattc ttcatcactc tgcataggag actgattggg aatcatgaca
     4501 actttttgac caacctcgta ggttccggtc gggtcagaaa tgacggttcc acatgactcg
     4561 tgaatcattg ccattggaag ctttttattc aaaatcttgg gatcacgttt tccctgatag
     4621 taacgctgat ccgcatgaca gacagccatg tagttgggac ggataaggat atgattctct
     4681 tggtcaatag cctcttcctg atatttgaca ttgataaact taggcttagt tagttgataa
     4741 atttgattaa tcattttact agtctttctc aatcatactt tttgcaatct tcaaatctgt
     4801 tacggttgta atcttcagat ttgagtattc acctttggcc aaggccacat cttttccttt
     4861 gatcacaaag attttacatg catctgtcaa gatttccttc tcttcatcag aaagagatcc
     4921 ataaaggtcc atgaagtcct tgcaacggaa tgtttgaggt gtttgtcctt gataaaggtg
     4981 agcacgattt ggaatatctg taataaattg accattggta ctttcaacga tagtatcaac
     5041 cgcttctacc actgtgtcca ctgcgtcatg attttgggca agttggatac tgtcctgaat
     5101 catgcgaagt gtaataaatg gacgaacaga atcgtgggta acaacgatat cctctggagt
     5161 aagcggacga taagcatcaa tggcttcaat gattttctta atacttgtat tgcggtcagc
     5221 accacccttt gtaatgatga tacgttcctt ataaagagga agatatttat ctacaagatc
     5281 ttctgcatga gaaacccagt ctccatgaac accaactaca attttttcaa tacttggctc
     5341 caagacaaat ttttcaattg tatgaatcaa aataggtcga tcacctagct ctaaaaattg
     5401 ttttggcaag ttactgatcc ccatgcgtgt gccagttcca ccggcaagaa ttcctgcata
     5461 aatcatctat tttctccttt gtcttactaa aaaaacttat tctctctatt ataccacaat
     5521 ctagtcctat cataaaagaa atactggtcc tccccttcta gaataggaga attatgcagt
     5581 tctattattc aaagaaacta gtccatttct agtaatgact aggaattctg tagcaattac
     5641 taataaatgg ctgcgaaatt ttagagtcct tcagaataat atactttcct taaagaaaac
     5701 ttaaaactta agatttgaaa cttcaaagtg attacttgac ttactgccct tcatttctta
     5761 ttagctaact ttatgatata atgaaaaacg aggtaaattg aatgaaaagt ataaaattaa
     5821 atgctctatc ttacatggga attcgtgtct tgaatattat ttttcccatc ctaactggaa
     5881 cctatgtcgc gcgtgtcttg gaccgaactg actatggtta cttcaactca gtcgacacta
     5941 ttttgtcatt tttcttgccc tttgcaactt atggtgtcta taactacggt ttaagggcta
     6001 tcagtaatgt caaggataac aaaaaagatc ttaacagaac cttttctagt cttttttatt
     6061 tgtgcatcgc ttgtacgatt ttgaccactg ctgtctatat cctagcctat cctctcttct
     6121 ttactgataa tccaatcgtc aaaaaggtct accttgttat ggggattcaa ctcattgccc
     6181 agattttttc aatcgaatgg gtcaatgaag ctctggaaaa ttacagtttt ctcttttaca
     6241 aaactgcctt catccgtatc ctgatgctgg tctctatttt cttatttgtt aaaaatgaac
     6301 acgatattgt tgtctataca cttgtgatga gtttatcgac gctgattaac tacctgatta
     6361 gttatttttg gattaaaaga gacatcaaac ttgttaagat tcacctaagt gattttaaac
     6421 cactctttct ccctctgaca gccatgttag tctttgccaa tgccaatatg ctcttcactt
     6481 ttttagatcg cctcttcctc gttaaaacag ggattgatgt caacgttagt tactatacca
     6541 tagctcagcg aattgtgacc gttatagctg gggttgtaac aggtgcaatt ggagtgagtg
     6601 tgcctcgtct cagttactat ctggggaaag gagacaaaga agcctatgtt tctctggtta
     6661 atagaggtag tcgaatcttt aacttcttta tcattccact gagttttgga ctcatggttt
     6721 taggaccaaa tgccatccta ctttacggta gtgaaaaata tatcggaggc gacatcttga
     6781 cctctctctt cgcttttcgt acgattatcc tggccttaga taccattctt ggttcccaaa
     6841 ttctctttac caatggctat gaaaaacgta tcacagtcta tacagtcttt gctgggctac
     6901 tcaatttggg cttgaatagt ctcctctttt tcaaccatat cgtggctccc gaatactact
     6961 tactgacaac tatgttatca gaggcttctc tacttgtttt ctatatcatt ttcatccata
     7021 gaaaacaact catccacttg ggacatatct ttagctatac tgttcgatac tctctctttt
     7081 cactttcctt tgtagcaatt tatttcctga ttaatttcgt gtatcctgtg gatatggtca
     7141 ttaatttgcc atttttgatt aatactggtt tgattgtctt gctatcagct atctcttata
     7201 ttagtctact tgtcttcaca aaagatagca ttttctatga atttttaaac catgtcctag
     7261 ccttaaaaaa taaatttaaa aaatcttagg agtttaaaat gaaacaacta accgttgaag
     7321 atgccaaaca aattgaatta gaaattttga attatattga tactctctgt aaaaagcaca
     7381 atatcaacta tattattaac tacggtactc tgattggggc ggttcgacat gagggcttta
     7441 tcccttggga cgacgatatt gatttatcca tgcctagaga agactaccaa cgatttatta
     7501 acatttttca aaaggaaaaa agcaagtata agctcctatc cttagaaact gataagaact
     7561 actttaacaa ctttatcaag ataaccgaca gtacgactaa aattattgat actcgaaata
     7621 caaaaaccta tgagtctggt atctttatcg atattttccc tatggatcgc tttgatgatc
     7681 ctaaggtcat tgatacttgt tataaactag aaagcttcaa actgctgtct ttcagtaaac
     7741 ataaaaatat tgtctataag gatagccttt taaaagattg gatacgaaca gccttttggt
     7801 tgctccttcg actggtttct cctcgttatt ttgcaaataa aatcgagaaa gaaattcaaa
     7861 aatatagtcg tgaaaatggg cagtatatgg cttttattcc ttcaaaattt aaggaaaagg
     7921 aagtcttccc aagtgctacc tttgataaaa caatcgattt accctttgag aatttaagcc
     7981 ttcctgcacc tgaaaaattt gatactattt tgacacaatt ttatggagat tatatgaccc
     8041 taccaccaga agaaaaacgc ttctacagtc atgaatttca cgcttataaa ttggaggatt
     8101 aggatgcaat atttagaaaa aaaagaaatt aaagaaattc aactagccct gctggactat
     8161 attgatgaga cttgtaagaa acatgatatt ccttattttc tcagttatgg aaccatgctt
     8221 ggagccatcc gccacaaagg tatgattcct tgggatgatg atattgatat ttccctttat
     8281 cgtgaggatt atgagcgttt actgaagatt attgaagaag aaaatcaccc tcgctacaag
     8341 gttctttcct acgatacatc ttcttggtac ttccataatt tcgcatcgat tttggatact
     8401 tctactgtta tagaagacca tgttaagtac aagcgtcatg ataccagcct tttcatcgat
     8461 gtcttcccaa ttgatcaatt tacagacttg agcattgtcg acaagagcta taagtatgtg
     8521 gcccttcgtc aactagctta catcaaaaaa tcacgagcag ttcacggtga tagcaaacta
     8581 aaagattttc ttagattatg tagctggtac gctctccgat ttgtcaatcc tcgctacttt
     8641 tacaagaaaa ttgatcaact agtcaaaaat gctgtaacca acattcctca atatgaagga
     8701 ggagttggga tcggtaagga agggatgaaa gaaatcttcc cagttgatac ctttaaagaa
     8761 ctgattttaa ctgagtttga gggccgtatg ttgcctgttc ccaaaaaata tgaccaattt
     8821 ttaacccaga tgtatggcga ttatatgaca ccaccatcaa aagaaatgca agagtggtat
     8881 agtcatagca ttaaagctta tcgcaaaaac tgattgaggg ggattataca aactactaag
     8941 atagaggtta ttcaaaaaca taattttagt agaaaatgaa atacatattc ccacaataaa
     9001 acgcatcata tcaaggtttt tgaaaaacct tgatatgatg cgttttataa ttttaaagac
     9061 ttttttctat agtagattga aataagatgc gaacaaatca attagaaaat tcaaattaat
     9121 ttctagaaat attttagtat tcctgtgtac tgttctaaat tcagtctgct atatcttatt
     9181 tttctattta aatcgcttct gtaacaaagc tacgactttc aagtacctta agcatggcat
     9241 tagctgtatc tagcgctgtg aagaggggca ccccgtgttc aatggctgaa cgacgaattt
     9301 gctcaccatc ttcgtcagca gttcgttttg ttcctactgt gttaatgata gcttgaattc
     9361 ttcctttgcg tacaaaactt gggatatcct tatcgtcatc accaatctta ccaacaggtt
     9421 gggcttgcaa gccatggcta gcaaagaagg ctgctgtccc ttctgtcgca aggattccat
     9481 aaccaatgtt ttggaaacga cgagccaagt tcaaggcttc ttctttggca tcatcagcga
     9541 tggtaaagac gacattacca aaagttggca agtgtagata agaagcttca aaggctttat
     9601 agagagcttt ttccaaagta gcatcagaac ccataacttc acctgttgac ttcatttcag
     9661 gaccgagcaa gctgtctacc ttagctagtt tggtaaagga gaagacaggt gccttgatat
     9721 gaacacgggt gctttcaggg taaagtccat tttggtagcc aagttctgat aaactttgac
     9781 caagaatgag tttggtcgct acttgagcca taggaatatt ggttacctta gataggaatg
     9841 gaacagtacg gctggcacgt ggattgacct caataacgta gactttttca tccttgataa
     9901 caaactggat gttcatcatt ccaaggcagt gaagaccgat tgctaagcgt ttggtgtagt
     9961 ctgcgatggt ctcctgaacc ttttgcgaca aggtttgtgg tgggtaaaca gccattgagt
    10021 cacctgagtg gacaccagca cgttcgatat gctccatgat accaggaatg agtacatttt
    10081 taccatctga aatggcatca acttcgcact cttgcccaac gatataagag tcgacaagaa
    10141 ctgggtggtc tggactagcc ttaacagcag ttcgcatgta agaacgaagg tcttcttcgt
    10201 tttcaacgat ttccatggca cgtccaccaa gtacataaga tgggcggaca agaactggga
    10261 agccaatctt gcgagctgca agagctgctt cttcttcatt ggtagctgtt tgtcctggtg
    10321 gctgtggaat atccaattct ttgagagctt gctcgaagag gtcacggtct tcggcacgat
    10381 ctaggtcagc aacctgtgta ccaaggatgg tcacacctgc ttttgccaat ggctccgcaa
    10441 ggttgatggc tgtttgacca ccgaactgaa cgataactcc ctttggttgt tccaagtcaa
    10501 tgacgttcat aacatcttcg aatgtcaatg gctcaaagta aagcttatct gatacagaga
    10561 agtctgttga aacggtctct gggtttgagt tcatgatgat agcttcataa ccagctgcct
    10621 ggatagcctt aacagagtga acggttgcgt agtcaaactc aaccccttga ccaatacgga
    10681 ttggacctga acctaggaca agtacagatt ctttatcaga tctgatagat tcattttccc
    10741 aaccataggt tgaatagaaa tatggcgttt cggagtcgaa ctctgccgca caagtgtcta
    10801 ccatcttata aactggaaca atcttgtttt ccaagcgaag ttggcgaact tgatcatcag
    10861 tcgttcccca gagttcagca atcttacggt ctgaaaaacc attaagtttg gctgttttca
    10921 aaacttctag atcttgtgga tgagcaccca attcttgctc aatttcaaag atatgcaaga
    10981 gtttatcaag atagaagata tcaatttttg taagctctgc aatttcttca ggtgtgtagc
    11041 cacgacgaat tgcttctgat acgtagaaga gacggtcatc ttgggctttg acaacctttt
    11101 caatcaaggc atcatcagaa actgctgcaa gttcaggtat ttcattgtgg tgcaccccaa
    11161 tttcaaggga gcggcaggcc ttgagaagag attcctcgat gttacgaccg attgccatga
    11221 cttctccagt cgccttcatt tgtgtaccga gacggcgttc acccttttca aacttgtcaa
    11281 atgggaaacg tggaatctta gcaactacgt agtcaagggc tggttcaaac atggcatagg
    11341 ttgaacctgt aactgggttt ataacctcat ccaaggtcaa acctactgca atcttggcag
    11401 ccaacttagc aatcggatat cctgtcgctt tagaagcaag ggctgacgaa cgtgatacac
    11461 gagggtttac ttcgataaca taatacttga agctgttagg atcaagagct agctgaacat
    11521 tacatccacc ttcaatcttg agggcacgaa taatgctcaa gctcgcatca cgaagcattt
    11581 ggttttcata gtctgacatg gtttgcgcag gggcaaatac aatggaatcc cccgtgtgaa
    11641 tcccaactgg gtcaaagttt tccatgttac aaacaaccaa ggcattgtca gctgagtcac
    11701 gcatcacttc gtattcaatt tccttgaaac cggcaatcga acgctcaatc aaacattggg
    11761 taacaggtga caatttcaaa ccattttcag tgatttcacg caattctttc tcgttggcac
    11821 acataccacc accagtacca ccgagggtaa aggctggacg aacgatgact gggtagccaa
    11881 ttgtcgctgc aaaggcaact gcttcttcta ctgtgttaac aatttcagat tctggaatgg
    11941 gttgttcaag ctcttccatc aattgtttaa agaggtcacg gtcctccgct tggtcaatgg
    12001 cagataattt ggtacccaga agttcaacgc caagctcatc taagatacca tttttagaca
    12061 attccatggc catgttgaga cctgtctgac caccgagtgt tggtagcaag gcatctggac
    12121 gttccttacg aagaatacgt gtcacaaact caagtgtaat cggttcaatg taaaccttgt
    12181 cagcaatttc cttgtccgtc atgatggttg caggatttga gttaaccaaa acaacctcat
    12241 aaccttcctc tttcaacgac aagcaagcct gagtcccagc gtagtcaaac tcagcagcct
    12301 gaccaataat aatcggacca gaaccaatca ccataatttt ttgaatatca gtacgtttag
    12361 gcatagttta tgatacaaag cccgtaaaga acacagtgaa aataggaaac tcggcgcaga
    12421 cgccttcagc gtcaagacga tgtttatctt tttcacacag ttcttagggc gtgttcactt
    12481 ccgcgaacaa tgtatcttct cctttctatt cgtcgcctct cagggcgaca ttaaataaga
    12541 tacaaaggac gactagaaag cgattgaatt ttaggaaatc aaggaaggat tgacaatcca
    12601 agttggtttc tctaaattct gagctttccg tccatgttca gttacataaa ttctccgacg
    12661 agcttttact cattcttagt ttgattgttt aaaaacttcc atcatctcga taaactcgtc
    12721 aaataggtag ctagcgtcgt gtggcccagg agctgcatct gggtggtatt gaacagagaa
    12781 agcaggttgg tatctgtggc gcacaccttc cactgacttg tcattgattt cttcgtgggt
    12841 aataatcaag tgctctggca aatcctcgcg gctgactgca taaccatggt tctggctggt
    12901 gaagtctact cgtcctgttg cgatttcacg taccgcatgg ttgaatccac ggtggccaaa
    12961 cttcatctta taggtcttag ccccgtttgc cattgcaaag agttggtgtc ccatacaaat
    13021 accaaagatt ggaatttttc cttgtacacc gcgaatcatg tcgagtgctt gtggaacgtc
    13081 ttctgggtta cctggaccat ttgacaacat aactccatca ggattgagat ggagaatttc
    13141 ttcagccgtt gtcgaataag gaacaactgt cacgttacag ttgcgtttag aaagttcacg
    13201 taggattgag tgcttgagac caaagtccac tagcaccacg ctcaaaccaa ctcctggagc
    13261 tggataagac gttttagtag aaacctgttt gatattgtct gtcggtaaaa ctgttgcttg
    13321 gagctggtcc gtcacatggt ccatactgtc cccaacatgg gtcaaggttg cacgcatagt
    13381 accatgctta cggataatct tggtaagagc acgcgtatca attcctgaaa tccctggaat
    13441 tttcttggct ttcaaaaatt catccaaggt catttggttg cgccagttgc tagctctacg
    13501 cgcttcttca aaaacaacga ctcccttaca agttggaata atggattcat aatcatcacg
    13561 attaatacca taatttccta ccaaaggata agtaaaggtc aagatttgtc cattataaga
    13621 ctggtctgta atggattctt ggtagccggt catccctgta ttaaagacga tttcgcctgt
    13681 tacatcaata tctgctccga aggccttgcc ttcaaaaact gtgccatctt ctaatactag
    13741 aattcttttt gtcatatttt cacctctcgt ggacgctcac tggcgtcttt taacgtcttg
    13801 tgttttagtt ggcgtttcta ctcgctagta cggattctaa gattgccatt cgaacaaaga
    13861 caccattggt catttgttgg acaatccgtg attttggtgc ttcaaccaag tggtctgcta
    13921 tttctacatc acgattgatt ggagctgggt gcatgaggat tgctgtttct ttcaaacgat
    13981 cgtaacgttc ttgagtcaag ccatgttggg catgatagtc ttcttttgaa aatacagctc
    14041 cactatcatg gcgttcgtgt tgcacacgga gaaacatcat gacatcaacc tgatcaatga
    14101 tttcatcaat ggttacaaac tttccatagt ctgcaaactc ttgacttctc cattcctcag
    14161 gtccagcgaa aaagagttca gctcccaagc gtttcaaaat ctgcatattg gatttggcaa
    14221 cgcgtgagtg gtccaagtca cctgcaatag caactttaag accctcaaag tggccaaatt
    14281 cctcataaat ggtcatcaaa tcaagcaagc tctggctagg gtgttggccc gaaccatctc
    14341 caccattgat gatggaagtc gtaatcgttg gactagcaat caattctcta tagtagtcga
    14401 cctctggatg gcgaatcaca cagacatcca ctcctaaagc agacagagtc aaaatggtgt
    14461 cataaagtgt ctcaccctta ttaaccgaac tagtcttcac atcaaagtca agtcgttcca
    14521 atccaagttt aatctctgcg acttcaaagg acttatgtgt ccgtgtagaa tcctcaaaga
    14581 agagattgga aacaatcgga tggtcttcat agggaagctg ggctccattt ttaaactcaa
    14641 ttcctcgctt gatcaatttc attacttgat cgacagtgag gtcttccatg gacaccacat
    14701 ggttcaatgc ttgttgattt tctgacatgg ctactccttt aactttctaa gcttcttcag
    14761 taatcagaac tctgtcttgg tcatcaagtt ctgtcatctc tacgatgatt tcttcagaac
    14821 gactggttgg gatatttttt ccaacgtaat ctggacggat tggcaattct ctatgtccac
    14881 gatcgactag aactgctaaa ctcacacgcg caggacgacc atgaccgaca atattatcaa
    14941 tagcagcacg gatggtacga cctgtataga gcacatcatc caccaagata acttcgcggt
    15001 ctgtcacatc gacagaaacc aaagaagtat cttctccact tttaacatca tcacggaaag
    15061 gtttagtatc caattccaca acaggaactg aaagattttc taactgcttc aaacgttctt
    15121 ggattcggtg ggcaataaag acaccacgag ttttaatacc agccaagacg atcttattca
    15181 aatctttgtt gcgttcgata atctcataag taatacgcgt aatcgctcgt ttgacggtca
    15241 attcgtctac aacttctttt gttttcatga caaacctcca aaaagaaaag tctccttaaa
    15301 caaggagact tgaaatttat agccaagcga gccctactgc aaacagtata gacttcaccc
    15361 ttctacttta tcgcgctcct tgcctgcctc acgggacagg tttaaaggaa tatttagtta
    15421 tcatttacta tagcacaaag catgcttaaa atcaagcaaa aagtttcaat gtagcatctt
    15481 acaaattgct aaaatcatat aattgtgggt actggtcaca ctctggattt tttggatggc
    15541 aaatggctct tccaaaataa atcatggcct gatgggcagc taaccactgc tcaggcggca
    15601 agatatccat gacccgcttt tccacctcaa gtggcgtcgc tgattttttg acaatatcgt
    15661 ggtgtttgca aatacgctcc acatgagtat ccactgcaaa ggctggaatc ccaaatccta
    15721 cactcatgac aacattggct gtcttgcgac caacacctgc caaactctcc aattcttcac
    15781 gtgtctgagg gacttgacca tcaaaatcgt ctagtaactg ttgggcacat tttttaagga
    15841 atttagcttt attccgatac aatcccaagc gagaaatatg tgaagcaatc tcactctctg
    15901 tcgctacaga catagcttgg ggtgttggaa aggcaacaaa gagacctggt gtggccttat
    15961 ttaccgctgc atctgtcgtc tgggctgata acatgaccgc aaccaggagt tcaaaatgat
    16021 tggtaaaatc aagactaggc ttggcatctg ggaagagggc aatgatttct tctagcacct
    16081 ttcgtgctcg tttttttgac aagaccatta ttcatctccg tcaaatagtc cttgtaagcc
    16141 agcaaaagga ctgttttctt ctttctttac tgcttgttga gcttggtatt cttcctctgt
    16201 catgatttgc cagtcatttc ctgagataaa tccttgacca gcttcttctt cagccgtcaa
    16261 gaccttgata ggaatgttta gcaggatatt gtctgataca ctctcagcaa ggtcaagctc
    16321 cccattttca atgggcaaga ccaagtcatc atctaaaact tcttgatcta gctggttagt
    16381 tgcgccttcc atgaaaactt ccgtgactgg ataagattca actaactcaa ctggctccat
    16441 actgcgactc gaagcaagaa caatggtata agatagttga taatctaaga aatacatacg
    16501 gtcttcatat tgtactttcc caactgcaag gatatctttt acatctaaaa tttcttgatt
    16561 acgtgcacgc aggtcatcaa ctaaatctaa cgtttgttca aagttcaaac cttcagactg
    16621 cttacgaatt tcttgaatat ttaatttcat acttcctcca taaagattta ctctcttgat
    16681 tataccatga aaaggctaca aatcagcaca ccaaactttg taattaaaat tcaaaatttt
    16741 aacatattta ctatgacagt tttatttttt agtgctatac tatagggaaa gagtacatca
    16801 gatcaaggag gatgctcata tggaagacat ggaagacaag aaactcattc aactcctatc
    16861 caagttaaat aaaagctacc aaaactgtaa acagggtacg gcagatgata ttcgactaca
    16921 agagctgcta aacactacta tgcaagagct caaaaaaacg gaaaagttga acaacagtat
    16981 cttaattgat cttgagaaat tttaccaacc caccagtcct ctgattggac tgggtagcct
    17041 aaaactaaac gatcaagcac gcactgcttg gcgaaactat gataaaatcc attacgagca
    17101 tgtcaaacac gtactaagtc tctatggacc tgtttttgga ttttagagca tagaatttcc
    17161 agttttctgt tgacaaaatt tccttaaaag tatagtataa agatactaat actcggaggt
    17221 aagggagaca tgaacaacta agtctatcaa ataaagaacc tttatttagt agatcttgtt
    17281 tttgtctctt tttgtgtgct cttttatgct ctttttctgg catgttaata gagttttttt
    17341 gacatagact ttgggctcta ctaggtaaag tagagctttt tgttatgcac tatcgacatt
    17401 ctagaaaggg caatagtatg ataaaaatca atcacctgac catcacacaa aacaaagatt
    17461 tacgagacct tgtctctgac ctaagcatga ccatacaaga cggggaaaag gtagctatta
    17521 ttggcgaaga aggaaatggt aaatcaacct tacttaaaac tttaatgggg gaagatttgt
    17581 ctgatttcac tatcaaggga gacatccagt ctgattatca gtcactggcc tacattcctc
    17641 aaaaagtccc tgaggaccta aaaaagaaaa ctttacacga ctacttcttt ttagattcta
    17701 ttgatttaga ctacagtatc ctctatcgtt tggcggagga attgcatttt gatagcaatc
    17761 gtttcgcaag tgaccaagag attggcaatc tatcaggggg cgaagctttg aaaattcagc
    17821 ttatccatga gttagccaaa ccctttgaga ttctattttt agatgaacct tcaaatgacc
    17881 tagaccttga gacagttgat tggctaaaag gccagattca aaagaccagg caaaccgtta
    17941 ttttcatttc ccatgatgaa gactttcttt ctgaaacggc agacactatt gttcacttgc
    18001 gactggtcaa acaccgtaaa gaagcggaaa cgctagtaga gcatttagac tatgatagct
    18061 atagtgagca gagaaaggct aattttgcca aacaaagtca gcaagctgct aacaaccaaa
    18121 gagcctacga taaaaccatg gaaaaacatc ggagagttaa gcaaaatgta gaaactgcgc
    18181 ttcgagctac caaagatagt actgccggtc gcctattggc taaaaagatg aaaactgtcc
    18241 tctcacaaga aaaacgctac gaaaagacag ctcagtccat gactcaaaag ccacttgaag
    18301 aggaacaaat ccaacttttc ttttcagaca tccaaccatt accagcttct aaagtcttag
    18361 tccaactgga aaaagaaaat ttgtccattg acgaccgtgt tttggctcag gaactgcaac
    18421 taactgtccg tggccaagaa aaaatcggta tcatcggccc aaatggtgtt gggaaatcaa
    18481 ctctgttagc caaattacag caacttctga atgataaaag agagatttca cttggtttta
    18541 tgccacaaga ttaccacaaa aaactgcaat tggatttatc ctcaatagct tatatcagca
    18601 aaactgggga aaaagaggaa cttcagaaaa tccaatctca cctagccagt ctcaatttca
    18661 gttatccaga aatgcagcat caaattcgct ccttatctgg cggacaacag ggaaaactcc
    18721 tgcttttgga tctagttctg cgcaaaccaa actttctcct gctggatgaa cccacacgaa
    18781 acttttctcc cacttctcaa ccccaaatca gaaaactctt tgcaacctat ccaggcggtc
    18841 tcatcactgt ttcgcatgac cgtcgtttct taaaagaagt ctgctcgatc atctatcgca
    18901 tgacagaaca cggtttgaag ctagttaatt tagaagattt ataaatttgc aacatagcaa
    18961 aaatccagag acgacctctg gattctttta catctgtttt aaacgttcaa tccgttctga
    19021 gataggtggg tgggtataaa agagtttttg gaacccccca cctttcttag gatcattgat
    19081 ataaagggca ctgctagcat catcgacgtg gcgactcata ggtttgctat tgtccaactt
    19141 atctagggca ttaatcattc cctggggatt gcgagtcagc tcgacactag atgcatctgc
    19201 cagaaattcc ctctgacgag aaatagcgag ctgaaccaag gttgcagcga gaggtgccag
    19261 tacaatagct agtagggaaa ccactagcat aatgatttca agaccatttc catctcggtc
    19321 atcatcactt cgtctgcgac ctgctccacc ccaccacatc atacgacctg ccatactaga
    19381 aagcatggtg atagcactag caagggcaac tgcaatagtc gaaatacgga tatcataatt
    19441 acgaatatga ctgacttcat gtcccataac agcttctagt tcttcacgat tcatgatagc
    19501 tagtagacct gaagtcgcag caacagccgc attttgagga ttagaacctg tcgcaaaggc
    19561 atttaaggct ggatcatcaa tgatgaaaac acggggcata ggaatctgag cgaccagagc
    19621 catatcttcc actacatggt agaggtctgg tgccgtttgc tcatccacct cacgcgctcc
    19681 attcatggac atgacaatct ctgtcgattg aaaaatcata gacaaagcgt agataaagcc
    19741 gataatcagt gcgataacca aaccaccaag tccagatctt ataaagagat aaccaaccgc
    19801 ataaccaaca agagctaaga gtaggaaaaa taccagcaac aaaatccagg tttttcgttt
    19861 attgcttgca atttgatcaa acaacatctt agtcacctaa accgctaaaa tcaactttag
    19921 gaaccgactt ttcctcttca gatgtttgaa ggaaatctgc cgctttaaat ccaaacattc
    19981 cagcgataat attgctcggg aaagtttcta attttacatt gtagttgctg acaacactgt
    20041 tatagagttg acgagagtaa gaaattttat tttctgtgtt tgtcaactcc tcttgcaatt
    20101 taacaaagtt agcactagct ttcaaatctg gatagctttc tgcaactgca aaaatacctg
    20161 aaacctgacg agtgagggca tcactggctt tcatagcttc tgctggtgaa gtcgctgccg
    20221 ccacttggtt acgtagttct gccacctttt caagggtaga accttcatat ttggcataac
    20281 cttttacagt ctcaatcaag tttggcaaga ggtcatttcg acgtttcaac tgaacatcaa
    20341 tctgactcca agcctccttg gtttgcatac gatttttaac caaaccgtta tagctaacaa
    20401 tcacaaaaat aacaataaga gcgataactc caagaataat ccaagtcata atataagtcc
    20461 tttctgcttt tagattagta ccagtatatc aaattttcta tgattgtggt aaaataagat
    20521 gatactaaag aaggaaataa ctatgaaacc aaaaacattt tacaacttgc ttgccgagca
    20581 gaatcttcca ctttcggacc agcaaaaaga acaatttgaa cgttattttg agctcttggt
    20641 cgagtggaat gagaagatta atttgacggc gattacggac aaggaagaag tttatctcaa
    20701 acatttttac gattcgattg cacccattct tcaaggtttg attcccaatg aaactatcaa
    20761 acttcttgat atcggggctg gggcaggatt tcctagtcta ccaatgaaaa ttctctatcc
    20821 ggagttagat gtgaccatta ttgattcact caataagcgc atcaacttcc tacaactctt
    20881 ggctcaagaa ctggatttga acggagttca tttctaccac ggacgtgccg aagattttgc
    20941 ccaagacaag aacttccgtg ctcaatatga ttttgtaaca gctcgtgcgg ttgcccgtat
    21001 gcaggtccta tctgaattga ccattcccta ccttaaggtt ggtggaaaac tattggcact
    21061 caaggccagc aatgcgcctg aggaattatt agaagctaag aatgccctca atctcctttt
    21121 tagtaaggtc gaagacaatc tcagctacgc cctaccgaat agagatccgc gctatatcac
    21181 agtggtagaa aagaaaaaag aaacaccaaa taaatatccg cgtaaggctg gtatgccaaa
    21241 taaacgccca ctttaaattt tttagtaaac aaatgtttac aaaatcagcc tcgctctttt
    21301 atttctaggc tcgggaaaaa atgatttaca aaatcagcct cgctctttta tttctaggct
    21361 cgggaaaaaa tgatttacaa aatcattttt ttctgctata ctatcctaag caaaggtttt
    21421 taatgtcatc ccgtgaggtg acgaagacgc agaaatattt aaaactcttt aaaatctaaa
    21481 ttttaaagaa gtcttactct gagggcctat tgctgtaaaa taatgggctc ttttttgatg
    21541 cccaaaagtg aggtttatat gaaacaagaa tcaactgttg atttgttact agacgttgac
    21601 caacgtcctt cggctggaaa aggaattctc cttagtttcc aacacgtttt cgccatgttt
    21661 ggtgcgacca tcttggtacc attgattttg ggaatgcctg tatctgttgc cctttttgct
    21721 tcaggtgttg gaacactcat ctacatgatt gctactggtt ttaaagttcc agtttatcta
    21781 ggttcttcat ttgcctttat cacagctatg tcactggcta tgaaagaaat ggggggggat
    21841 gtatctgctg cccaaacagg ggttatcttg actggtttgg tctatgtcct tgttgctacc
    21901 agcatccgat ttgtaggaac aaaatggatt gataaactct tgccaccaat cattatcggt
    21961 cctatgatca tcgttatcgg tcttggactt gcaggttcag ctgttaccaa tgcaggtctt
    22021 gtagcagacg gaaattggaa aaatgctctg gtagccgttg ttactttcct aattgctgcc
    22081 tttatcaata caaaaggaaa aggcttccta cgaatcattc cattcctctt tgccattatc
    22141 ggtggttacc ttttcgcact aactcttggc ttggttgact ttacaccagt tcttaaagcc
    22201 aactggttcg aaattcctgg tttctacttg ccatttagca caggtggtgc ctttaaagag
    22261 tacaatcttt actttggtcc agaagccatc gctatcttgc caatcgctat cgtaacaatt
    22321 tctgaacata tcggagacca tactgttttg ggtcaaatct gtggtcgtca attcttaaaa
    22381 gaaccaggtc ttcaccgtac tcttcttggt gacggtatcg caacttctgt ttctgccttc
    22441 cttggtggac cagccaatac aacttacgga gaaaatacag gggttatcgg tatgactcgt
    22501 atcgcttctg tctcagttat ccgtaacgct gccttcatcg cgattgccct cagcttcctt
    22561 ggtaaattca ctgccttgat ttcaactatt ccaaacgctg tacttggtgg tatgtcaatc
    22621 cttctctatg gggttatcgc cagcaatggt ttgaaagtct tgattaaaga acgtgttgat
    22681 ttcgctcaaa tgcgaaacct catcatcgca agtgctatgt tggttcttgg acttggagga
    22741 gctatcctta aacttggtcc agttacactt tcaggtactg ccctttcagc catgacagga
    22801 atcatcttga acttgatctt gccatacgaa aataaagact aagagtctaa atacacctaa
    22861 tccactcaga cagctgagtg gatttttcgt ataccataat aaaagtgtct taacaaaatt
    22921 attaaaatca aaaaacgtat aatatcagat attctaaaac cttgatactg tacgttttat
    22981 catagaaatt tttactttat tttctcatca aatgagattt gcatcaatct cttgtcttac
    23041 ttgcgtttct tcttcgcttt cttcattttg ttagccatac gtttcatgga ctgtttcatg
    23101 gcaaattcac caattttacc tttcaaaccg ccaccaaaca tctggctcat atctggcatt
    23161 cctgctcctc cgagagctga taagtcaggc ataccgcctt gtcccatcat tccttcaagg
    23221 gcagacatat ccattcctcc catatttggc atatttttag gaaggttatt tggattaatc
    23281 cccatttgct tcatcatttt attcatatcc ccagacataa caccctgcat gagctgttta
    23341 gcctggttaa agtccttgat gaatttattg acttcgacga atgtatttcc agaaccagca
    23401 gcaatacgac ggcgacggct tggatttaac aaatctgggt tttcacgctc ttcaggtgtc
    23461 atcgaagaca caatggcacg tttacgagca atctggcgtt catccacctt catgttttga
    23521 agggctggat tgttggccat acctggaatc atcttgagca agtcttccat cggccccata
    23581 ttttgcacct gatctaattg atcgatgaaa tcattaaaat caaaggtgtt ttcgcgcatc
    23641 ttctcagcca tttcaagggc tttttgttca tcgtattcct gagaagcttt ctcaatcaaa
    23701 gtgagcatat cccccatacc aaggatacgg ctagacatgc ggtctgggtg gaaggtttca
    23761 atgtccgtaa tcttttcacc tgtaccagtg aacttgattg gttttccagt aatgtgacga
    23821 acagacagag cagcaccacc acgagtatcg ccatcaatct tggtaaggat gaccccagtc
    23881 acttccaact gagcattaaa ctcacgcgca acattggctg cttcctgacc aatcatagca
    23941 tcaacgacaa gcaagatttc atttggttga gccaatgctt tcacatcacg aagctcattc
    24001 atgaggagct catcaatctg caaacgaccc gcagtatcaa tcaagacata gtcgttatga
    24061 ttagtttggg cttgctccaa accttgacgt acaatctcaa cagctggtac ttctgttcca
    24121 agtgcaaaga caggcacatc aatctgttgt cccaaggtct taagctggtc aatggcagct
    24181 ggacgataaa tatccgccgc aatcatcaaa ggacgagcat tttcttcttt cttgagtttg
    24241 ttggccaatt taccagcaaa ggttgtttta ccagcccctt gtaaaccaac catcatgatg
    24301 atggttggaa tcttaggtga cttgataatt tctgccgtat cagaacctaa aacggctgtc
    24361 agttcctcat caacgatttt aataatctgt tgcgcaggat taagtgtatc aatgacctca
    24421 tgcccgactg cacgctcacg aactttcttg ataaagtcct ttacaacagg caaggcaacg
    24481 tcggcctcga gcaaggccaa gcgaatttct ttggttgcct cttggacatc agattcagag
    24541 atttttcctt ttttacgtag atttttaaag acgttctgca aacgttctgt taaactttca
    24601 aatgccattt ttcttcctct tattctctat tatcaatgct tgttaaaatt tctatctgct
    24661 cctgcagaaa gtcatccttg ggatagcgct ccaaaatctg atcaaaaatc tgactgcgga
    24721 caatatagtc cgagtacatg tgcaatttca tctcataatc ttccagaatc ttttctgttc
    24781 gcttgatatt gtcatagaca gcctgacgac tgacaccaaa ctcctcggca atttcagcaa
    24841 ggctgtaatc atcagcgtag tagagctcga tataattcat ttgcttatct gtcaaaagcg
    24901 ccgcataaaa ttcaaagagc gcattcatac gattggtttt ttcgatttcc ataactttta
    24961 ttataccaaa aattagccta atctaccaca ctaggaagcc gatccaagaa gatagatagc
    25021 taaatttgaa aaagacatga gcctagcccc aagtaatttc caattgatag ctggcaaaag
    25081 gatgtccctc ttgattttgt agttgataat ctagttcaat cttttgccta tcaacttgat
    25141 aatggctcgt ttggatgata aactcctgca tgcccatagg tgtaggaata taggctaaac
    25201 tatcgctatc ctttagaaag cgcataatgg tcttgggatt agaaaatcgg ctcatcataa
    25261 gttcttgacc atgaaattta atcactactt tttccttttc ctcattatag aaaagcaggt
    25321 agctataatc tcctttttca tgcacttcca catcataaag ctggtcaatc acttccaact
    25381 gctcatcaaa ctgaattgta tttcgcatcc gaatcttcac atcaggccct ctttcttgtc
    25441 tcttgtccta ctattttacc aaaaagagca ggattttgct ataatggtca tatgaacgaa
    25501 aaagtattcc gtgacccagt tcacaactac atccatgtca ataatcaaat catctatgac
    25561 ttgattaata caaaagaatt tcagcgtttg cgccggatca aacaactggg aacttccagt
    25621 tataccttcc acggtggaga acacagtcgc ttctctcact gtctaggagt ctatgaaatt
    25681 gcacgacgca tcacagagat tttcgaagaa aaatatcctg aggaatggaa tcctgccgag
    25741 tctctcttga ccgtgaccgc tgctctccta cacgaccttg ggcatggtgc ctactcccat
    25801 acttttgaaa atctctttga tacagaccat gaagccatta ctcaggagat tattcaaaat
    25861 cctgagacag agattcacca agtcctgcta caagtggcac ctgatttccc agaaaaggtg
    25921 gccagtgtca ttgaccatac ctatcctaat aagcaggtcg tgcagctcat ttctagtcag
    25981 attgacgcag atcgcatgga ctatctcttg cgcgactcct attttacagg agcatcctat
    26041 ggggaatttg acctgactcg catcctccga gtcattcgtc ctgtcgaaaa tggtatcgcc
    26101 tttcagcgca atggcatgca cgccatcgaa gactacgtcc tcagtcgcta ccagatgtac
    26161 atgcaggttt atttccaccc agcaacacgc gccatggaag ttctcctaca gaatcttctc
    26221 aagcgcgcca aggaactcta ccctgaggac aagaatttct ttgcacgaac ttccccacat
    26281 ctcctgcctt tcttcgaaaa aaatgtgacc ttgactgact atctggctct ggatgatggc
    26341 gtgatgaata cctacttcca gctttggatg actagtcctg acaaaattct tgcagattta
    26401 tctcaacgct ttgtcaaccg caaggtcttt aaatccatta ccttttcaca agaggaccaa
    26461 gatcaactta ctagcatgag aaaattggtt gaggacatcg gctttgatcc cgactactat
    26521 actgccattc ataagaactt tgacctccct tatgatatct atcgtcccga atctcaaaat
    26581 ccacggacac agattgagat tttacaaaaa aatggagaac tggccgaact ctctagcctg
    26641 tctcctatcg tccaatccct tgctggcagt cgccacggag ataatcgttt ctatttccct
    26701 aaagaaatgt tagaccaaaa cagcatcttc gcaagcatta cccagcaatt tttacacttg
    26761 attgagaacg atcattttac cccaaataaa aactagaaga ggaattttat gagtattaaa
    26821 ctaattgccg tcgatattga cggaacccta gtcaacagcc aaaaggaaat cactcctgaa
    26881 gtcttttctg ccatccaaga tgccaaagaa gctggtgtca aagtcgtgat tgcaactggc
    26941 cgccccatcg caggtgttgc caaacttctg gacgacttgc agttgagaga ccaaggtgac
    27001 tatgtggtga ccttcaatgg tgcccttgtc caagaaactg ctactggcca tgagattatc
    27061 agcgaatcct tgacctatga ggattatctg gatatggaat tcctcagtcg caagctcggt
    27121 gtccacatgc atgccattac caaggacggt atctatactg caaatcgcaa tatcggaaaa
    27181 tacactgtac acgaatcaac cctcgtcagc atgcctatct tctaccgtac ccctgaagaa
    27241 atggctggca aagaaattgt taaatgtatg tttatcgatg aaccagaaat tctcgatgct
    27301 gcgattgaaa aaattccagc agaattttac gagcgctact ccatcaacaa atctgctcct
    27361 ttctacctcg aactccttaa aaagaatgta gacaagggtt cagccattac tcacttggct
    27421 gaaaaactcg gattgaccaa agatgaaacc atggcaatcg gtgatgaaga aaatgaccgt
    27481 cccatgctgg aagtcgttgg aaaccccgtt gtcatggaaa atggaaatcc agaaatcaaa
    27541 aaaatcgcca aatacatcac caaaacaaat gacgaatccg gcgttgccca tgccatccga
    27601 acatgggtac tgtaaaagta tcatttttca ataagaattg attagcaata aaatccaatg
    27661 aattttttta gcaaactatt taatttaaaa caaaataatc ataatagaga cacaaattct
    27721 gattgtaaca atttttacct aaacgaatta gaatgtggcc ttactcctgg gcaactcata
    27781 ctcatagatt ggactcaaaa aacagggaga aattataatt tcccaagata ttttaaatac
    27841 tctcttcaaa ttgaccctga atctacacac aatcaattat acaaattagg atacttcact
    27901 aaaaataaga ctttatcata tcttacagta gtagaattaa aaactatatt atctaaacat
    27961 aatttagcta cttctggaaa aaaagcagaa ttaattacaa gaataattaa taatgttaac
    28021 attgacaatt tagatattcc gttcgaattt aaactaacaa aagaagcaca aaatcttatt
    28081 atcgaacata gtgactatat caaagcatac tatgataaag acataagtat ggaagattat
    28141 tgtaaagaaa aaaacaatat ctcttttaaa gcaacttttg gtgatataaa atggagtctc
    28201 ttaaataaac aagctcatag gaatactgta tcaggagatt ttggatgctt atctaacaca
    28261 cgaaaggctc agggaagaca tttggaacaa gaaggtaata ttaaacatgc tttaatatat
    28321 tacatagaat ctttgataat tactatttca ggattagaaa acaatttttc agccactgat
    28381 tatccagtat attatcccga ttcgatacct gactactcac taaaacatat tcaaacatta
    28441 atggaatcat tatctgatga cgattatgat tttgcttttg atgaagcatt atttcgcttc
    28501 tcaattttga atgcaaatca ttttttatct aaggaagata ttgactattt aagagttaat
    28561 ttacctcgtt ccactgctga agaaataaac aattacttaa agaaatatga atgttatagt
    28621 cctttaaata atttagaact tgacgatttt gaataaattg actatacaaa catttatata
    28681 ctcgatatag tctcaatttt atctgatgat tgcccaaatt tttcaataat aaaacgcata
    28741 atattatgga gacaatcccc tatattatgc gttcttttaa tatcaaagac tctttgacaa
    28801 acttctttga tatctaatta catgccccct gcaggaatcg aacctgcaac tactccttag
    28861 gagggagttg ttatatccat tgaactaagg gagctagata aaaactctgc tgaaaaagca
    28921 gagtttttta gtcgaattaa cgacggattt ctttgatacg agctgcctta ccttgaagag
    28981 cacgcaagta gtacaatttc gcacgacgta ctttaccgta acgtacaact tcgatttttt
    29041 caacacgtgg agtgtggatt gggaagatac gctcaacacc tacaccgtta gagattttac
    29101 gaactgtgta gttttcagag atgccagcac ctttacgtgc aataacaaca ccttcaaaaa
    29161 tctggatacg ttcacggtta ccttcgacaa ctttcgcatg tacacgaaca gtgtcaccag
    29221 gacggaatga tgggatatct gtacgaagtt gaccttcagt caagctttgg attaatggat
    29281 tcattttatt ctcctatctt tgtcaatctt gaggaatctt cctcagcgga taaactgtat
    29341 ttttgtgcgt ccattacaca caagatatag tttaccaaat ttctaaaaaa aagtaaagaa
    29401 atttatagca aaattcctaa aaaaatcgca accaaaccac ctatgtaagt caaagtcagg
    29461 taagaataaa agaccttctt atcacttagc agtctttgaa gttcgtcatt caaggtcgaa
    29521 aaagttgtta aaccgccaca aaatcctgtt gctagaatag catatacttc cttagactct
    29581 acatgattgt agaatactcc aattaaaaaa caacctaaaa gattagctat gagcgttccg
    29641 agtggcaatt tagaagcttg attatagcga gaaaagaaat accgtacgag tgctcccaag
    29701 ccacaggcga ttgcaagata gacgattacc atttcttcct ccccaaatag taagctaaaa
    29761 gcaggcctcc accgatactc aaaagcaaat acaggactaa actaaaataa cgccctgtat
    29821 caagcagctt cacagtatca agcattaggc tggaaaaagt tgttaaacct ccgcaaaaac
    29881 ccgtccccag tgctaaaatc aagcccttac tagtcccctt atagaccaga tagcccttga
    29941 caagaaaaat caagcagaaa attcccagat agttgacaaa gagagtgccc caaggaaagt
    30001 ctggactggc tggtaaccag gtggaaacta gatatcggac aagtccgccc aacatagcag
    30061 ctagaaaaat tcctagcgga taaaattgtt cttttttcat ttgatatttt gatcctgata
    30121 atcacgcgaa cgtttgagta tgtacgaaaa agtcgcgaca atagtctcct gatagcgctt
    30181 gtcctctaca cgactcctaa ccttttcaaa aataacttct tctctcttag tatctaaaat
    30241 cggtttacct gaagctttct tataagcaac caccccctca actaaatgca ttcgttcttc
    30301 taacagtttg acaatttggt cgtcgatttg atcgatttct tgacgaataa tatctaaatc
    30361 catacagtct cctcctttat ttgaattatt gtatcaaaaa gccatcaatt aggctagtaa
    30421 aatcccaact ctcgataaga aaaatgcact gattgattac atcagcgcac ttttatgctt
    30481 atcctacttt agcagccaat tcttctgcga attgttccaa gcgttcaata tcttcttcct
    30541 cggcagaaag atcaacttta acacactctg aacctttttc tgctcctgtt gacacaaaga
    30601 cacggtcaaa gtcatcaaca gccttacaga attcatcgta gaaggtatct cctgagccga
    30661 ccactccgta gattttgcca ttcaagttga gatctgctag gtcttcgtag aagtccatca
    30721 tctcatctgg caattcacca tcaccataag tataggtcgc aacgatagcg atgtctgctt
    30781 ccaagaagtc tgaagcgtca acagttgtac attcatcaac atcaacatcc aagcccaagt
    30841 cacgtaattt gtctgctaca atatctgcaa tttcttcggt attaccggtc atactggcaa
    30901 atacaatttt tgctaatgcc atatcgtcct cctcaattta tctttctcca ttatatcaca
    30961 tctttttcat tttaaaaata aaaattcttg tgctacaatg aaatgagaaa gaattgaggt
    31021 tatttatgga gatttgccaa caaattttag aaaaaattaa agaatacaac accattatca
    31081 ttcatcgtca tatgaaacca gaccctgatg ccttgggaag tcaggtggga ttgaaagcct
    31141 tgctggaaca tcatttccca gaaaaaacca tcaaagccgt cggttttgat gaaccaactc
    31201 ttacttggat ggctgagatg gatcttgttg aagatagagc ctaccaaggc gcacttgtca
    31261 tcgtctgtga tacagctaat actgctcgta tcgatgataa gcgctatagt caaggtgatt
    31321 ttctcattaa gattgaccac catccaaatg atgatgtata cggtgacctg tcttgggtcg
    31381 atactagttc aagtagcgct agcgagatga ttaccctatt tgcccaaaca acccaactag
    31441 ccttggcaga tcgcgatgct gagttgctct ttgcaggaat tgtcggtgat acaggtcgct
    31501 tcctctaccc ttctaccact gcacggactc ttcgcctggc tgcttatttg agagaacata
    31561 actttgactt tgcggctctc actcgcaaaa tggacactat gagctacaaa attgctaaac
    31621 tgcaaggcta catctacgac catctggaag tggatgaaaa tggtgctgct cgcgttatcc
    31681 tgagtcagaa aatcttgaaa caatacaatg taaccgatgc tgaaactgcg gccattgtag
    31741 gtgcacctgg gcgtattgat agtgttagtc tctggggaat ttttgtggaa caggctgatg
    31801 gccactaccg cgttcgctta cgcagtaaaa tccatcctat caatgaaatc gccaaggagc
    31861 atgatggtgg aggccaccct ctagcaagtg gtgctaattc ctatagccta gaagaaaatg
    31921 aaatcatcta ccaaaagtta aaaaacttgc ttaaaaactg ataaaatact tgccaaactt
    31981 ttcagaatct gatagactag tatggtaaca atctatggct cgcaaagaga ccatggcaga
    32041 aaggaaatat tgcaaaatga aaaaagatat ccatccagaa tatcgcccag ttgtcttcat
    32101 ggacacaact actggttacc aattccttag cggttcaaca aaacgctcta acgaaacagt
    32161 tgagttcgaa ggcgaaactt acccattgat ccgtgtggaa atttcatcag actcacaccc
    32221 attctacact ggacgtcaaa agttcactca agcagatgga cgcgtggatc gtttcaacaa
    32281 aaaatacggt ctcaaataat gataaaagaa cagtttcgac tgttcttttt tgtttcttga
    32341 aatcaactgc tgttttcatg ttccagactc atctgtaggt tcggtttcca tgctactagg
    32401 caggaaggaa atagctgttt caacacgtcc ataatgagct gtactattgt cacgaaccac
    32461 actttcattg atggtccaag tggaattcat tttcttaaaa gcttctcgga ctttttccaa
    32521 atctttggag gcaatggcct gttccaaggc ttcaaaacga ggacttatac tcatctgctt
    32581 tcaaaaagca ttctagtcca tctccgatta acgatggact ttatcacctc cttctccagt
    32641 ccttgtataa catcttggag ttgattcatg acatcttcca aagtttaaaa ggctttattc
    32701 ttaaatccac gtttacgaat ctctttccac acttgttcaa tgggttcatc tctggtgtgt
    32761 atggaggaat aaatgcaaag ccaatattag tcggaatctt taagg
//
LOCUS       DAVBCV010000020        31002 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBCV010000020 DAVBCV010000000
VERSION     DAVBCV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31002
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.020"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(298..516)
                     /locus_tag="VQ516_000901"
     CDS             complement(298..516)
                     /locus_tag="VQ516_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2511366.1"
                     /translation="MVLNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(762..1040)
                     /locus_tag="VQ516_000902"
     CDS             complement(762..1040)
                     /locus_tag="VQ516_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511367.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEMLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1254..2885)
                     /gene="rlmD"
                     /locus_tag="VQ516_000903"
     CDS             complement(1254..2885)
                     /gene="rlmD"
                     /locus_tag="VQ516_000903"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2511368.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2959..3933)
                     /locus_tag="VQ516_000904"
     CDS             complement(2959..3933)
                     /locus_tag="VQ516_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2511369.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4007..4621)
                     /gene="pmp23"
                     /locus_tag="VQ516_000905"
     CDS             complement(4007..4621)
                     /gene="pmp23"
                     /locus_tag="VQ516_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2511370.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4621..5595)
                     /locus_tag="VQ516_000906"
     CDS             complement(4621..5595)
                     /locus_tag="VQ516_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2511371.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5603..6859)
                     /locus_tag="VQ516_000907"
     CDS             complement(5603..6859)
                     /locus_tag="VQ516_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2511372.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6921..7352)
                     /locus_tag="VQ516_000908"
     CDS             complement(6921..7352)
                     /locus_tag="VQ516_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511373.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7354..7956)
                     /locus_tag="VQ516_000909"
     CDS             complement(7354..7956)
                     /locus_tag="VQ516_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2511374.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7940..8779)
                     /gene="prmC"
                     /locus_tag="VQ516_000910"
     CDS             complement(7940..8779)
                     /gene="prmC"
                     /locus_tag="VQ516_000910"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2511375.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8779..9858)
                     /gene="prfA"
                     /locus_tag="VQ516_000911"
     CDS             complement(8779..9858)
                     /gene="prfA"
                     /locus_tag="VQ516_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2511376.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9855..10445)
                     /locus_tag="VQ516_000912"
     CDS             complement(9855..10445)
                     /locus_tag="VQ516_000912"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2511377.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10568..10750
                     /locus_tag="VQ516_000913"
     CDS             10568..10750
                     /locus_tag="VQ516_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2511378.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10887..12260
                     /gene="mnmE"
                     /locus_tag="VQ516_000914"
     CDS             10887..12260
                     /gene="mnmE"
                     /locus_tag="VQ516_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2511379.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12307..13242)
                     /gene="dapA"
                     /locus_tag="VQ516_000915"
     CDS             complement(12307..13242)
                     /gene="dapA"
                     /locus_tag="VQ516_000915"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2511380.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13298..14374)
                     /locus_tag="VQ516_000916"
     CDS             complement(13298..14374)
                     /locus_tag="VQ516_000916"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2511381.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14716..14788)
                     /locus_tag="VQ516_000917"
     tRNA            complement(14716..14788)
                     /locus_tag="VQ516_000917"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14753..14755),aa:Thr,seq:ggt)
     gene            complement(14888..15397)
                     /locus_tag="VQ516_000918"
     CDS             complement(14888..15397)
                     /locus_tag="VQ516_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2511382.1"
                     /translation="MKKLTIRDIADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15395..15491)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15520..15951
                     /locus_tag="VQ516_000919"
     CDS             15520..15951
                     /locus_tag="VQ516_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2511383.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            16033..16410
                     /gene="mscL"
                     /locus_tag="VQ516_000920"
     CDS             16033..16410
                     /gene="mscL"
                     /locus_tag="VQ516_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2511384.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16452..17546)
                     /gene="hemH"
                     /locus_tag="VQ516_000921"
     CDS             complement(16452..17546)
                     /gene="hemH"
                     /locus_tag="VQ516_000921"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2511385.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17624..18456)
                     /gene="srtG2"
                     /locus_tag="VQ516_000922"
                     /pseudo
     CDS             complement(17624..18456)
                     /gene="srtG2"
                     /locus_tag="VQ516_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(18534..19532)
                     /gene="srtG1"
                     /locus_tag="VQ516_000923"
     CDS             complement(18534..19532)
                     /gene="srtG1"
                     /locus_tag="VQ516_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET2511386.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19550..20746)
                     /gene="pitB"
                     /locus_tag="VQ516_000924"
     CDS             complement(19550..20746)
                     /gene="pitB"
                     /locus_tag="VQ516_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET2511387.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20791..21342)
                     /gene="sipA"
                     /locus_tag="VQ516_000925"
     CDS             complement(20791..21342)
                     /gene="sipA"
                     /locus_tag="VQ516_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET2511388.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21342..23297)
                     /locus_tag="VQ516_000926"
     CDS             complement(21342..23297)
                     /locus_tag="VQ516_000926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET2511389.1"
                     /translation="MDAGIGTGTNINAGLTEAQRLLQSARAGAKKVVILLSDGEANMY
                     YESNSGRTIYNYYSNPNVGRMIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYY
                     IGSNDSITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDV
                     LSKYVQLLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLE
                     DDAKYVLKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSR
                     TKIKDYSEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDS
                     YRKVTSPVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYR
                     QLPSLTVKKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQE
                     TALSYLPRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVT
                     GAFANLLQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKI
                     LDLPLNARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSST
                     LVLGVVLPLGGIFFIILLGHLVVNRRK"
     gene            complement(23427..23930)
                     /locus_tag="VQ516_000927"
     CDS             complement(23427..23930)
                     /locus_tag="VQ516_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET2511390.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
     gene            24289..25512
                     /gene="pepT"
                     /locus_tag="VQ516_000928"
     CDS             24289..25512
                     /gene="pepT"
                     /locus_tag="VQ516_000928"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2511391.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25548..26177)
                     /locus_tag="VQ516_000929"
     CDS             complement(25548..26177)
                     /locus_tag="VQ516_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511392.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            26142..26363
                     /locus_tag="VQ516_000930"
     CDS             26142..26363
                     /locus_tag="VQ516_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511393.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(26634..26982)
                     /locus_tag="VQ516_000931"
                     /pseudo
     CDS             complement(26634..26982)
                     /locus_tag="VQ516_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27087..27647)
                     /locus_tag="VQ516_000932"
                     /pseudo
     CDS             complement(27087..27647)
                     /locus_tag="VQ516_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27873..30992)
                     /gene="phtE"
                     /locus_tag="VQ516_000933"
     CDS             complement(27873..30992)
                     /gene="phtE"
                     /locus_tag="VQ516_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2511394.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         8835 a         6360 c         5686 g        10121 t
ORIGIN      
        1 aaaaggatct agtttttatt tttagttaaa ttatgtctat atttttcatt aactcatcaa
       61 taaaaatttg tttgctaata tctctttctt ctgattttgt ttcaaggatt attgctacat
      121 cattcatatt tttaggtaaa taccaaccat ctggcttatc tgaatatccc ttgaagccta
      181 attgattaaa agtagttatt tgaccagtac cttgattgat gttttcttca acttcattaa
      241 agcctatact tctcttgcat aatccctaac ttgatcttct gtccttaaag tcattttcta
      301 ttcctcgtca ctaaaattat cttcaaaact aataatatca ttaggagtgc aagataaagc
      361 cttacatatt ctttctaaat tcttaaatgt aattggttta tcctttccca tttgtgccat
      421 aacatttgtt gtaagtcctg ccatagctat tacatctgtt ttctttagtc ctttttttgc
      481 taactgtatc cataatggtt tatagttaag taccataata tccctctttt tttcttcatt
      541 aaatttattt aattatagca taaatattga tttcattcaa tcttacattg atttagtgat
      601 ttgttttaga gaatttgttg tgttattccg tccttatttt gccataaatt gttttaagtg
      661 ggacctctta tccttaccat ttttacctcc taattttgag tataaaaaag acgatagagt
      721 ttttaatttc tatcgtctga gatttacata ttaaattttc atcacaaaat aagactcttt
      781 aaagaatctt taaattcatt ggtatgctgc ttacaatatc cattattaaa atggatacta
      841 taagcagcac accaactatt tattttttga gttttaaact ttggtagaag tcttgaactc
      901 cttatattta cataactatt tgcctctata actattatga cacgaaacca acaagcaagt
      961 tcaagcatct ccatatcttt attcaaatga ggactaagat attcacaaac tttatcataa
     1021 acctctttat cttttttcat aaaaggggca tacaacttag caaatttatc ctaaaatttt
     1081 ttgttgcctt tatcaatcat ttcaacgctc ctttaaatat aaaaataatc ctatcaatct
     1141 ttatattgag cctattttct aattgatacc ttgcaagaaa ggaataatag ctgttcctat
     1201 accaagtagg aatgtaataa atgcagaaag tcatatactg tcattctttt ctattaaatc
     1261 attttgaagt gtctcaaagc atccatgatg gcttcttctt tttcaggtgt acactgtgga
     1321 ataatttgtt tatccttttt agacttgttg taatgttctc gtaattctat tccacatttc
     1381 ttttttatct gtgcaatata taatgtcgaa acttttaatt caaatttatt ccaaacatat
     1441 tctttgattt gagcatatgt tgctttgctc tccgcacttg tcaaatccat ctcatccagc
     1501 tcaatttcaa cacttatgtg cttatcgaca tcgagtttgg acaaaagtgc taccgtctcg
     1561 acgtgatgcg tttgaggaaa tagatccacc ggctggactt tcttcaattc atatcccaat
     1621 tcttggtaga gtttgatatc acgcgccatg gttgcgacat tgcaggagat ataggcgatg
     1681 cgatcggctc ctgtttgggc gcttgctttg ataaagcttt ctgtcaagcc cttgcgtgga
     1741 ggatcaacca agataacggt tggttgaata ccttccttga gccatttctt catggcattt
     1801 tcagccgtgt cacagacata gtgggcatta gtaatcttgt tcaaagaagc attcttctgg
     1861 ctattctcaa ccgcttctgg aatcagttca acaccgtaga cttctttgac atgcttggcg
     1921 actgataaac caatggttcc aataccagaa taagcatcaa taaccacgtc atcttttttt
     1981 aactctgcaa agtcaatggc tgtttgatag agtttctccg ccatttcagt attgacttgg
     2041 taaaaggctg ggccagcgat ttggaagtca tttcccaaca tctggtccgt aatatagtct
     2101 tgaccataaa gagtgcacca ctccttacca aaaatcgcat tggtattctg gtcgttgata
     2161 ttttgcatga cagacacaat ctctgggaac tgcttgataa cttgttcaat caattggtca
     2221 acacgaaaaa cttttggacg agttgtcacc aaaacgacca tgatttgtcc tgaatagtga
     2281 ccacgacgca ccacaagatt ccgaatcaat ccagactgtt ccttttcgtc ataaggtttt
     2341 aaatcaaaac gacggagcag gtctcgaaga gctactacga cttggtcaat gacaggatcc
     2401 tggataaaga aatcttcaag gggcatgagg ttgtgcgaat tcttacggaa aaatcctgtt
     2461 tccaagacac cattcactcg acgaacgggc acctgcgcct tattgcgata cttgactgga
     2521 tgttccatac caagcgtttc agcaacttct acatctgcaa ttccagcaat cttgtagaga
     2581 ctgtccttga cttgcttggt tttaaacttg agctgttctg gataagaaag gtgtcctaaa
     2641 tccgcgattc ctgaacgcag gtaagccaaa tctagatctt gattacggtg tagtgactgg
     2701 acaaggtatt tttcaacttt tccaaagcca atctttttat tgaccttgag gacacgcatg
     2761 agaatttttt cactcggtaa agcattctct acaaaaaaga ccaaaccatc taccttggca
     2821 actcctgccc cttcatgggt caaatcaaca atttcaactt ctacaatatc atttttcttt
     2881 aacattcttt tctctttcta ttggccgaca aaaaagacgg caacgttacg attaccatcc
     2941 attataccat gaaatttctt aagaaccaaa actcttgaag aagttgacaa gggcttgcca
     3001 gagggagctt aagaagttac caccgtcctc tagcgcctta tccgcatcaa agttaaggtt
     3061 gatattttta aaactgtcgc cagcttgtga tacgatgctt tgtttaaggt catttagggt
     3121 tttagtgaaa tctgcattgc tgaggatatc actctttgag agattcaagg caaaattgat
     3181 gatgatattg atctggtttc ctgttatgac ctgatcaagt ttgtaatttt ttaaggtatc
     3241 ttcaacaatc ttgcggacat cttcttctgt cagatttccc ttgctttctt tagctttggc
     3301 gagtcctgac ttgatatcag ctagggcaac gtttaattta ttagcatcat agcctgattt
     3361 gtccttgttt tcagcattga tatctgacaa agcctttagc tcttcttgag ccaaatcttt
     3421 attggcttgc ggcaccttgg ctccattagc ttctagcgaa tagtagatcc cagccaaagc
     3481 actctcacct gtaactggaa taggggctgc tacagtgatt ttggcatgtt ccatacccag
     3541 cgttactgct gcgtttcggt acatatcctg agtcacctta gtgatatttt ctggtgtttc
     3601 aatcttgacc tcaagtggcg atttgtcacc tagcttttga atcttggctg atgaatacaa
     3661 ctgtaagcta gagtcattgg ccacattcat gattttagaa taaacatcag gtgtcatggt
     3721 cttgagttct ttggtatctg ttgaggcatt gtagcccagt tttttaagag tttgattttt
     3781 ttggtcttca gatagggagg aacctaggac atattcaggt tggacatagg tttcatcgat
     3841 aaccttttga acatctgttg ctgcatggac gctattcata gctgttactg cccacaagac
     3901 cgcagcactg gtcagaaaga gtttctttct catagggaat ttcctccttt acttttctag
     3961 agtaatatat ctatcttaaa gaaaacttat aacaaaaaca cctggtctag ccagatgttg
     4021 aaaagagagt gaaacatttg atgatgtaaa ggttaagtcg tacctgtcta gaataataat
     4081 agtttcctcc atttacatag agttcagcac cgtgaaaaat ggaaatgggg tgaatataac
     4141 tataagtctt tccagtcgta ttaccaagca agggggcaac agtctcacga gagtactgtt
     4201 tggctagagc caaggtattt tccttgccat tttgggcgat aaaatcgata taggcaggtc
     4261 caaaattata ggcttgaaca gctgtccaga tatctacccc cttcttctgc gccagataga
     4321 gattgcctgt cagagtttga atgccttgcc gaatgctaga ggcattatca ttgatggtgt
     4381 tggttgaacc acttgcagac tcactagact gcataacatc gccttctttt ccttttgttt
     4441 cagtataaat catagcaagc acaagctctt cgtttgctgg ggtgtctttt tcactcaaga
     4501 tttctcgcac catgggttga taggtcatga cttgtttgac atcttgatga acgcggtaag
     4561 ctttatagcc agcaaaaagg aagactgcta gtacaagcac tcttcgaatt cgtttaaaca
     4621 ttatttactt tggatatcct cgatattttt gattaagata gagtaggttc cattttcctt
     4681 ttggataaac tcaacagact cggcgtcttg atagacgtta ttgggaacga tgagctcaat
     4741 tccatttgat aaggagagtt tttggttttc aaatttcttt aattggcgac tggcatcaat
     4801 ttcatcaaat tgaacaggtt ctggtacggc ttctttgact tggtcaataa agctcaaacg
     4861 agccgtcaga ttgttgtcaa aaaggtcatt agccaatttc tcaggtgaca attcattgct
     4921 ttcttctagg ttgttgaaaa tcgctgattt gaccttggat tgaaattgaa aatcatctgt
     4981 gttaaaagat tcagcaattc tctgggctgt tttttccagt tccttgatag attttttagg
     5041 agaaatctta ggagcgacag caagaagatt atctgaaaaa tagttcaaaa aagtcccgtt
     5101 gtacttgatt cgtttttcaa tcaggtgata cttgcgactc tgaagattga ccaccaaggc
     5161 ctcgtcagca cccgttccaa atccaggcag gttattctga gtcagcttga ttggattatc
     5221 aacttctcct ccgaggtggg tcaaggtctc ccgcagggca attcgcaaga aagcgaaatg
     5281 ttctacacct tctttagaaa attgaacaaa aatcaagtca ttggtcttga gattttcaga
     5341 aatgctaaac tcctctttcc agagattagc cagcgttact gatgtctcca acaaatcgtc
     5401 tgtaatatga ttgaagaagg gattttcttc ttcgaaaatc ccagtcttgg cttcatctga
     5461 atacacatgt tcaatttttt tacgtaggta ttcttcgatt tttggagtaa tattgagaaa
     5521 cttatctgct aagaacagct cggtatcatc cggactgaac tggtgaataa tggctttctt
     5581 aatataaatg tccataaaag ttttattcct cgtataatgg gaaggcatct gtcaattctt
     5641 tgactgcact tctcacttct tctaatacag cctcattttc tgaattctta agggttttaa
     5701 tgatgagttc agccactttg cgactttctt cttcaccaaa tccacgtgca gtaatagctg
     5761 ctgctccgat acgaatccca cttgtcttga atggtgacaa ggtttcgtaa gggattgaat
     5821 ttttatttag ggtaatattg acttcatcca gcaagttttg agcaactttt ccgttttcta
     5881 caaccttagt cacatcaaca aggaagagat ggttttcagt tccacctgaa ataatacgga
     5941 aatcagggtc ttgcaagaag acatctgcca tagccttgct gttcttaatt acattggcag
     6001 catattcctt gaaggctgga tccaaaactt ctttgaagga aactgcctta gccgccacaa
     6061 catgctctaa aggaccgccc tgaatacctg ggaaaatagc tgaattgatt tttttagcaa
     6121 gttcttcgtc attggtcaaa atcaaaccac cacgaggtcc acgaagggtt ttgtgggtcg
     6181 ttgttgtagt gatatgagcg tatggcactg ggcttgggtg aagaccagct gcaaccaagc
     6241 cagcgatgtg ggccatatca accataagct tcgcaccgac agcatctgcg atttcacgga
     6301 attttgaaaa gtcaataatt tgagaatagg ctgaagcacc agctacaatc agttttggtt
     6361 ttacttcttg ggcttgtttc aagatagcat caaagtctaa gagttccgtt tcaggatcaa
     6421 cactataaga aacaaagttg taggtttgac cagagaagct aacaggagcc ccatgagtca
     6481 aatgaccacc tgatgccaaa tccattccca taaccgtatc acctggctca atcaaggaca
     6541 tgtaagccgc acagttggcc tgacttcctg agtgaggttg gacattggca aatttagcac
     6601 cgaaaatttc ttttgcgcgt tcaatagcaa gagtctctac aacgtctact acatcagttc
     6661 caccataata acggcgtcct gggtaaccct cggcatattt atttgtcaag atagaccctt
     6721 gagctgccat aacagccttg gaaactacgt tttccgaagc aattaactcg atattatttt
     6781 gttggcgttc ttcttctttg gcaatagcat tccagagatc agcatcatat gctttaaaat
     6841 catctttgtc aaaaatcata ggtcttctcc tttattgtgt gactagtcca ttagtttgat
     6901 tttacaataa gaaaatcaaa ctaaaagatg cgaataaacc gtttctgcat tttatcacaa
     6961 gtatagccaa ctttttcata aaatgcatga gcacccagac gatgattggc agaatttaag
     7021 cggataaacc cataaccaca tcttttggct tcttgttcca acccttgtag taaactttta
     7081 ccgatacctt gaccttgcgc ttgaggtgaa actgctaaag ctaagatatt aaatcctgct
     7141 ttggaataga gtgattcgta aacttcagcg tggacatatc caagtaagac atgattagct
     7201 gcatcctcat agccaagtag gaaatgatgg gaatcctgag acagtctagc tagttggcta
     7261 gccgtttcct ctggactaaa agtataaccc aaagcctctt ggttgatgtc acatatcgct
     7321 ttcacatctg tttcttgcaa atctcttagc atttcaagcc tcctcaaaag aaatctctgg
     7381 caaccgagca agaatatctt ctcgtttaat tgcgccttgg cgtaagattt tcaccttgtc
     7441 tccagacaaa tccacaatag ttgaatcctg tccagttaga aaagcatcgt cttccagacc
     7501 cagaacctct tggtcaaaat ccttcagaat ttgttcaaag gttacaccac ttgcctgacc
     7561 tgagatattg gcagacggcc caatcaaggg acctgtctct cgaattaaat ccagtgtgat
     7621 agggtgactg ggcatccgaa atccaatagt tgcaaggtca gaatttaccc aatagggaac
     7681 tcggtcattg gcttcgagaa taatggtcaa gggacctggc aaaaaggtct ctacaagttt
     7741 ttgtagataa gttggctgat tcttagaaaa gtgcaagata tcctcgaaag aggcgatatt
     7801 gagattgagt gccttatctc taggacgacg tttgagttgg taaacatggt caactgcttt
     7861 ttcatctaag gccttggcaa aaagaccata aacagtctct gtaggtagaa cgacagctcc
     7921 accattttcc aactcttgtc taatcctgtc catcatcaac cacaaccatc ctattttgac
     7981 caaattggtc tttgagtgtt cgaacttttt tttcaggaag atgttttcta aaaagttcgg
     8041 gaacactttg accttgcttg tatccaattt caaggtaaat cttaccacca tccttgagat
     8101 agtcttttgc atcttccgca attctacagt aaatagctag gccatcctcg tctgcaaaga
     8161 gagctagatg aggctccgaa tgcaaaacat tcaagcctac ctctgactca tcttcacgag
     8221 agatataggg tggattggat acaattatat catatttttc agaaatttct gcaaaacaat
     8281 cagatttttt aaaaaatata ttaagatttt gatcgctagc attctctgat gcaagctcta
     8341 aggcctcttg agaaacatct gctgctgtca ctgaccaatc tggtctattt tttgctagag
     8401 cgagagcaat agctccacta cctgttccaa tatccagaac tgagagattc gtctcaagat
     8461 tttcagttag aataagctcc actaactcct ctgtttctgg acgaggaatc aaaacccgtt
     8521 catccacctt taaatgcatt ccataaaaat ctgcctgtcc aatgatgtac tgagctggct
     8581 tgtgagctgc tagttgctgg taaatatctt ctacaaattg tttttcttcc tctgttgtca
     8641 cctcctgctg gagggcaaaa ataaagtctg taaaagatag atttttcaga ctacgataga
     8701 caaaagagag gctttccgct tcctctcctt gtcttatcaa ctcttcttca aaatttgaaa
     8761 ataattgagc taatttcatt atttgtttaa ttcttctagt ttttgtgttt ggtcataaag
     8821 caccaaggca tccacaactt cgtccaattt accagacaaa atcgtatcta gtttttggag
     8881 ggtcaagccg atacggtggt ctgtgacacg gttttgaggg aagttataag ttcggatccg
     8941 ttctgaacgg tcaccagtac cgattgtcga cttacgctca gcgtcttgtt catcctgagc
     9001 aatctgagca aagtggtcag cgacgcgtgc acggataatc ttcatagcct tctcacggtt
     9061 cttctgctgg gtacgttctt cctgcatctc aaccttgata ttggttggca agtgaacgat
     9121 acgaacggca gtcgcaacct tattgacgtt ctgtccacca gcacctgatg cgtggtaaat
     9181 atcaacacga agatcttttg gatcgatatc gtattctact tcttcgactt caggcatgac
     9241 aagaactgtg gcagttgagg tatgaacacg accttggctt tctgtcacgg ggacacgctg
     9301 cacacggtgg gcacctgatt cgtacttaag cttagagtat acagactgac ctgaaaccat
     9361 agcaaccact tctttaaacc caccgacacc attcatagag gcttccatga cttcaaagcg
     9421 ccagccttgg gcttccgcat acttttggta catagttagc aaatctccag cgaaaagtgc
     9481 cgcttcgtct ccaccagctg ctccacggat ttcaaggatg atattcttgt catcgtttgg
     9541 atcctttgga aggagcaaaa ttttcagttt ttcttcatat tcttcttttt cagccttggc
     9601 atctttgagt tcttgcttgg ccatttcttc caagtccgca tctccgcctg attccttaat
     9661 catctcttcg gcatcgacga tattttgaag gacttgttta tactcacggt aggctattac
     9721 ggtatcacga ttggaagctt cttcttttga aagctccata aaacgcttgg tgtctgaaac
     9781 gacatcaggg tcactcagca attctcctag ttcttcataa cggtcttcta cagcttgtag
     9841 ttgatcatag atgttcattt ttcttcattc tccttattga tttcaggggc aaaataatgt
     9901 ttacggcaaa ccgagatata ggtttcatta ccaccaattt ggatctgttc tccatcgtaa
     9961 acgggcagtc catcctgtgt tcgcaacacc atggtcgcct ttttcttgca atactgacag
    10021 atggtcttga tttcgtcaat cttgtctgct aaaagcaaga gatatttgga accttcgaac
    10081 aattcattgc gaaagtcatt tttcaagcca aaagccatga cgggtatgtc taactcgtcc
    10141 acaacacgag ctaggtcata aacatggtgg cgtttgagaa actgggcttc atcgaccaac
    10201 acacagtaag gtttttctgg taggcctcgg atatagccaa agatatccgt tgtttcctca
    10261 atcgcaaggg cagggcgttt catgccaatt cgactcgaca catagccaac accgtcacgc
    10321 gtatccagag ctgaagtcat aatcacaaca ccttttcctt gctcctcata gttataggcc
    10381 accttgagaa tctcgattgt ctttccagag ttcatggtcc cataacgata atacaactgt
    10441 gccatgtttc ttgcttcacg tccatttcta aatttttgct acattctagt atatcataat
    10501 tttcttaagc tttaaacggc aaaatgtggt aaaatggaag aaatcaaaac tagtggagga
    10561 agctattatg ccatttgtac gcatcgattt atttgaagga cgcacgctcg agcaaaagaa
    10621 agctcttgct aaggaagtaa cggaagcagt tgtccgcaac actggagccc ctcaatctgc
    10681 tgtccatgtc atcatcaacg acatgccaga aggaacttac ttcccacaag gggaaatgcg
    10741 tactaaataa gctagcttaa gcagaattgc ttaggctttt tcaatctcca agtagcattc
    10801 attgaagaaa tatcctaaat ttgttacaat ttgaaaagaa acttggagaa tttccaagaa
    10861 aagagctatt aattaaagga aacattatga ttacacgtga atttgatacc atcgctgcta
    10921 tctctactcc actaggtgaa ggggctattg gtattgtccg cctgagcgga acagacagtt
    10981 ttgctattgc gcaaaagatt tttaaaggaa aagacttgaa caaggttgcc agccacactc
    11041 tcaactacgg tcacattatt gatcctctga ctggtaaagt catggacgag gttatggttg
    11101 gggctatgaa gtctccaaag accttcactc gtgaggatat tatcgagatt aacacccacg
    11161 gtggtattgc ggtgaccaat gagattctcc agctagctat ccgtgaagga gctcggttgg
    11221 cagaacctgg tgaatttacc aaacgtgcct ttttaaacgg tcgcgtagat ttgacacagg
    11281 ccgaggcggt gatggatatc atccgcgcca agacagacaa ggccatgaac atcgcggtca
    11341 aacaacttga tggttccctt tctgatctta tcaataatat ccgtcaagaa atccttaaca
    11401 cacttgccca agttgaggtc aatatcgact atcctgagta tgacgatgtt gaggaagcca
    11461 ctactgctgt tgtccgagag aagacaatgg agtttgagca attactaacc aagctcctta
    11521 ggacagcacg tcgtggtaaa atccttcgtg aaggaatttc aacggctatc attggacgtc
    11581 ccaacgttgg gaaatcaagc cttctcaaca acctcttgcg tgaggacaag gctatcgtaa
    11641 ccgatatcgc tgggacaaca cgagatgtca tcgaagagta cgtcaacatc aatggtgtac
    11701 ctctcaaatt gattgataca gctggtattc gtgaaacgga tgatatagtg gaacaaatcg
    11761 gtgttgagcg ttcgaaaaaa gccctcaagg aagccaactt ggttctacta gtgctaaatg
    11821 ccagtgaacc actgactgcg caagacagac aacttcttga aattagccaa gataccaatc
    11881 gcattattct acttaataaa accgacctgc cagaaacgat tgaaacttcg aaactacctg
    11941 aagacgttat ccgtatttca gtccttaaaa accaaaacat cgacaagatt gaagagcgaa
    12001 tcaacaacct cttctttgaa aatgctggct tgattgagca agatgctact tacttgtcaa
    12061 acgcccgtca catttccctg attgaaaaag cagttgaaag cctacaagcc gttaatcaag
    12121 gtcttgagct ggggatgcca gttgatttgc ttcaagttga cttgactcgt acttgggaaa
    12181 tcctcggaga aatcactggg gatgctgctc cagatgaact catcacccaa ctctttagcc
    12241 aattctgttt aggaaaataa gaaaaatcca tgatccttca ttcggtcatg gattttattg
    12301 tctttattag taatctggtc ttaagacccc tgttacagtt gccttagttg cttcgtagtc
    12361 gccatctacg acaaccttga taatgcgttt ggcatcttct tctggtgctg gaacaagagg
    12421 tagacgagtg ggtccagctt caaatcccat atagttaaga attgccttaa ctggagcagg
    12481 acttggataa gagaagagag cattaacctt aggaatgaat ttacgctgaa ttgctgcggc
    12541 tttcttcata tcgctttctg caatggcagt aaacatctcg tgcatttcat ccccatttgt
    12601 atgagaggca acagaaataa ccccatccgc cccaaggttc atggcatgga aagcatctcc
    12661 atcctcacct gtataaatca agaactcttc aggcttgtgc tcaatcaagt aagccatatt
    12721 agccaagcta gtacattctt tgacaccgat aatatttgga tggtcagcca agcgaagcat
    12781 ggtttctgga gtcaattcga caactacacg ccctggaatg ttatagataa taattggtag
    12841 gtcagaagca tctgcaatag tcttaaagtg ctgatacatc ccttcttgag aaggtttgtt
    12901 gtagtaagga acaatagcaa gcccagctgc gaaaccacca aattccgcta cttctttgac
    12961 aaactcaata gagtcacgcg tatcattagt acctacaccc gcaatcaaag gaacgcgtcc
    13021 attgacaacc ttttgtacag ccgcaaacaa ctccaactcc tcatcgtggg tcaaagttgg
    13081 actctcagca gtcgttcctg cgagaagaat tccatccgta tgatgggcca ataaatgctc
    13141 aatcaaggct ggaatagcat caaagttaat agaaccatcc tcatgaaagg gggtaataaa
    13201 ggctgtaatg attttacatt cttttaaatc ttgataagac atgaaaacac ctctctattt
    13261 caaagaagga gttcatctga actcctaaac gatatgacta ttttaattca aatttcaatt
    13321 cggctgttgg acgaaccaat ccacgttcat gaagagtttc agcaatctga actgagttcc
    13381 aagcagcacc tttgagaagg ttatctgaaa caacccacat gtgaattcct ttttctgcat
    13441 ccaagtcttt acggatacga ccaacaaagg tatcacgcga accaactgca ttgatagctt
    13501 gaggatagat ttgatgagct acatcatctt caagaacagc acctgggaag gctgcgatag
    13561 ctgcttttac ttcttcgatt ggagccactt cttttgtttc gatataaaca gactcagagt
    13621 gagctgacaa gactggaata cgcacacatg ttgcagatac tgcaatgcta tcatcttcca
    13681 taattttctt agtttccttg gtcatcttca tctcttcgta cgtgtaatca ttatcagtga
    13741 aaacatcaat ttgtggaaga gcgttaaagg cgataggata atgtttcttg tcaccacctg
    13801 aaggcaaaat ttccgcatgc aaatcacatg gtttcacacc atcattcaag acttcacgaa
    13861 gttcacgttg tgtctcaaga attgctccca taccagcacc tgaaacggct tgataagttg
    13921 aaacaatgat acggtccaag ccccattttt ggcgaaccgg ctcaagagcc accatcattt
    13981 ggattgttga acaattaggg caggcaatga ttccgttgtg ggcatcaagt gcatgagcat
    14041 tgacctctgg aacaaccaaa ggaacatctg gattttgacg gaaataagat gtattatcca
    14101 ctactaccac gccagctttt actgcgtatg gtgcatactt agctgatgta gaactacctg
    14161 ctgaaaagag agcaatatca actccttcaa aagctgtttc agtcgtttct tcaattgtaa
    14221 tatcttgatc tttaaatttc aatgacttac ctgctgaacg tgcagaagca aggtaacgga
    14281 ttttatcgat tggaagtgtt gattcttcca acatttttat catctgagca ccgacagcac
    14341 ctgtcgcgcc gactacagca actgtatatc ccataaataa cctctttcgg aattttctaa
    14401 aaatttctat aatagaagta ttatactact ttttccatga attgcaaagc tttttcatca
    14461 ttttttggaa aataaaaatc ccagtcgcta gactagggac taagaggcat cttcatgtga
    14521 tgagcaggtt cacacaactc atcaaggtcc gctcctgcgt tatgacctcc ttatgctcaa
    14581 tagtagtccg aagactacct atcgactcat cgcatattct atattactag agatacttga
    14641 ttttgtcaat aagaaatttt ttcgatcgag tataacaaaa aactctcgta ttcaagagtt
    14701 tccctataca tatcatgcta attgccggga tcgaaccggc gacctcatcc ttaccatgga
    14761 tgcgctctgc caactgagct aaatcagctt acttaagaag tatactatat tcagaaaaac
    14821 ttgtcaagtt tccttagaaa ttttccatag gaaaaagcca ggtaagaact acctagctta
    14881 tcttcttcta tttacttaaa tcaattcgac gaccaatttt accaataata atcgccccaa
    14941 taatcaatga agcaaattca ccgattcctg ttgtaaacca agtaaggaag aatggtaatt
    15001 ctgctacaat atttaactct gcagcgatag taaacatgga aattgaaaag aggattgaaa
    15061 agaagaaatg atcttttcga atcaatccgt taaagaggta atccttgcta tattttgcaa
    15121 aaagccaaac acctaaactg aggaatacta aggttgaacc accaccaaca aagacatcta
    15181 gcagtccgaa gctaaagaaa ttagcaatca tacaaccgat ggtaactccg atgatgtact
    15241 taggattgta aaaagccata aagttcatca tttctgaaat acgaaactga taagcaccat
    15301 agctgatggc atttaggggc ggtgtgacag tcaaaacgac gtagatagca gcgacgattg
    15361 caatatctgc aatatcacga atagttaatt ttttcatctt ttctcctttg gcggttgccc
    15421 gcgtgtaata tgcttggtga aagaagctaa gcaccaaggg ttgctttatt caaccctact
    15481 agtatagcac aatagcctgc tttatgctat acttaacaca tgaaaattcc tattcaaaaa
    15541 ttttttaaca atgaaatctt agcctatctc ttttttggcc ttgcgacaac tctagtttcg
    15601 attctctcac gactagtcat ttatcaacta agtcatcagg aactcctggc tactgcccta
    15661 gcaaatatta tcggtatcct atttgccttt atcacaaatg atacgattgt atttaagcaa
    15721 aaaaagaaaa atagacttat ccgtttagtg aaattttctc ttgctcgcct ttctactttt
    15781 ctgttagact tgtttttaac ttttctcttt gtaacccaat ttcctcacat tatagggcaa
    15841 ttcgtaactg aaaacattga taagataaat gctattgaaa caataattgc acaaatattc
    15901 attattatca tcaattatat attgagcaag gcctatattt ttagaaaata atgctccatt
    15961 tatgagcctt ttaattgcct tctataatga tttaggatac aatgaaacac gaaacattga
    16021 aaggaaaaca gtatgttaaa aaatctaaaa tcgttcttgc ttcgaggaaa tgttattgac
    16081 cttgctgtcg gtgttgtaat tgcctctgct tttggtgcta tcgttacttc acttgtaaac
    16141 gacattatca ctcctcttat tttaaatccc gctttgaaag ctgctaaagt tgaacgtatc
    16201 gctcaacttt cttggcatgg agtcggctat ggtaacttct taagtgctat tatcaatttt
    16261 atctttgtgg gtaccgccct cttctttatt atcaagggca ttgaaaaagc acagaaactg
    16321 actggcataa agaaagaaaa aactgccgaa aaaaaaccaa ccgaattgga agtccttcaa
    16381 gaaataaaag ctctccttga gaaaaaataa tcgattaaaa ggatctagcg caaagctaaa
    16441 tccttttttc tttactcttt gggtaacttg cctgcttttt caagcatttt tttgatcaga
    16501 taaggcatct taacattctc ccgccctttt ttctcaatca gttttttcac aaattctggc
    16561 atttgtaaat cttcagattc agctaaaatg tttttcgtct cgtctgaagg aactaactca
    16621 tcaaaggtct cttcttctgg gagaaattcc ttaaactctt gattttcatt gactctaact
    16681 gtattgacca aagcatcaat caaacgagaa tctgtatttg gcattggtgg acgatggtag
    16741 tttaccccaa attcctggca taagtcataa cattctacat cattgtcaaa caagacctca
    16801 atatgctcac tgatgaaact aataggtaca aaaatataat ggtctggatg ttctgtctgt
    16861 tctctgagat actccaaaac atctggttta atccatggaa taccaatatc actctcactc
    16921 tgccaagtgt tggtatattg ttctgaactc aagcccagtt tttcagcgac tagcttgcta
    16981 ttttcgaaaa tttggtctat ataaggatca ccaaagtcca aggcaaaaat gggcacacta
    17041 tgggctgaaa agatgacttt aaagctgtct tgctttactt cttcttttaa aatcttagca
    17101 atttcatctg cccaatagtt taagagcact tcttcctgat accagtcctt aatgaccaaa
    17161 aattgaattt gtttgctttc taaaaatttt tcatagccca tgacagagta gaaagaataa
    17221 tggggctcca aaatcaagca gatacattct tcaacaccat cagtttccat ctgcttgatc
    17281 acgtctggaa taaagggacg agaaaatttg ttggcaaagt agataccgta ctcctcccct
    17341 aaacgttctt tgactaaatt gacctcctca cgtgtaatcc gctgcaaagg cgtcccaccg
    17401 atacgaatat agttgtcata gagagtctgg atttcgtggt cttggggtct aactccacga
    17461 cgaatgtttg taaagaattc tgctactcct tcaaaactaa tctcctctgg agatccaaag
    17521 gtcatcatta atatggcttt tttcatgtta tcatccttat gatattattt tctctatcca
    17581 ttctatcatg aaagaaaaaa atagtaaacg tttcctacgt ctactatttc ttttgctctt
    17641 ttgttttttt atctcgtctt ataattagga tgataaggac aagaagtaag aggacgataa
    17701 taaggagtag aaaggctaga atgtaaatcc aatgactggg aattccgtga tcgaaatacc
    17761 gtgttcctac tgcttcccca ctttcatcct gtgctacttt caccggtgca tccgttattt
    17821 tagctaaaag gatatgtcgt ccattagtta tgttaaggaa acatctcgct tgtacttggc
    17881 tttacttagc agatattgat agtaagcctg gtgttcctca tccttggaac tcaaggttcg
    17941 ataaatctcc gtgtcatagg catccacttc taaaatccca aaaatttcaa gaccacgttc
    18001 tcgaccattg tagtagatag aaccgtagcg atgctggtcg aagaattcct tatcagcaaa
    18061 tttcttaatc tcaccgaaca tagcccctga agccatagag tgtccgtaga tgatcgtatt
    18121 aaaatcagta aagttgggat taaagcgata gtcgagaaaa attgatccca ttatcgtata
    18181 atcgccaaag gcattgcggt caaggtattt gacattatcc tgactttgta gaagcggata
    18241 gtctaccttg gtcccatcaa ggctaatcca accgcaaaca tctttgttca agtgctgtag
    18301 ggtttcaaaa cctgtgctcg aagcatcctt ttcaatagga ttatagtgat catagtcact
    18361 tgcttctact actctctgcc aactaaaaag agctacaaaa acacagaaga ataccagaaa
    18421 tacgttttta aatcgcgctc ttttttgaac cgtcatcagc actcctttct atgcgagatt
    18481 tcttaaaagg aaaggaggcg ggacaaaacc gcctcttttc tattcaccct tagtcagtaa
    18541 aagggtctgc ttcttgcatt ttttttactc tctcttctac gacgtaggat caagtagagg
    18601 acaaggagta agaaaatcaa gagcaagaac aatacaaaca agataaggta ccaaatccat
    18661 agtgggattg atgagaggaa acgtccaaga gacgagtcat caaagacact gtatggaaat
    18721 ggttttgatt tttttgtatg gaaagtattt ttagggacgg tgtctgtaat ctttgcgact
    18781 acgatatgac gaccattggt cacatcgaga aaacaggtac tgagtaggat gatacgatca
    18841 ctcggtgaga gtgagatatc ccgcttgtgc atggcgactg agagcaggtg gtctagatag
    18901 gcctgctggc ggtcctcacc ctgtattcct ggatcataga tgttaaagtc ataggcatca
    18961 acctccaaca tctcaaagat ctcgagccct ttttccacac cattgtagta tatactaccg
    19021 taacgatgct ggtcaaaaaa ttcctgatca gcaaacttag ccacatcacc aaacatgacc
    19081 ccattttcta cgtggtgccc gtagataatg gtattaaagt cttcgaactt aggattattt
    19141 cgtgcatcga gaaagatagc gcctgtcgct gcaaactcac ctttggagtc cttgttgaga
    19201 tacttttcat tgtccttggc ttggactaag ggatagtcga tattggtgcc atagacattg
    19261 atccaaccga ggacttcggg attgagttct tggagtttgc tgaagcctga aaaactggcc
    19321 agctcatcct gttgcgtcgt gacaggtcga taagcttcgt actcacttga cgaagctaag
    19381 gagtagactt ggttggaatc ccaaagggcg tagaaaccaa agagaaagat caaagctgca
    19441 aaaatgagca gcaagtgatt gatcacccca tccacagcaa gaacgagacg cctagacaga
    19501 ctcttttttg gtttctcacg cttggttttc atcatctgcc cccagccgtt taggcttctc
    19561 tacgtttttt agacaagaca acaaatccag ccaaaccaag accaatcatc aaaatgaatg
    19621 gaaggttatc aatcaagaga ccagtaggag taacgtcgtc gattttgtta gtaactttgt
    19681 tgtcgttttt cttttcacct atgaggatat ctgttaatgt aaaatctaca tttttttgtc
    19741 cttgagatgt ttgttctgca ccatttgtag tataaattga tgaatctgta taacctgcta
    19801 ctccagtttc agttactgta tatttagaac ctgctggtag ttgttcaact gtaaatgttt
    19861 caccatcttt aagagtaaaa ttgtactcaa ctgtatctgc tggggtcact actgccaaca
    19921 cctctgactt agatccatgt tttactataa tattcgtaac aggggtttct gctgttttat
    19981 ttgtacttgg aagttgaatc ttgatatgga attggaaatc tcttgtttta tcccctgaag
    20041 ctccatcaac ttttttagta attgttaatg ctttcgcatt ggggtctact tgactcggat
    20101 catcaggatt aggctcctta cttgcgtctt tggtatatgt gttatcaaat aaattataaa
    20161 cgccttgttc acttggttct actttagggg cgttagattt attattttcc ttaaagtata
    20221 ctgatgaaat atagacacca ccatttactt tattcttaac aataacgtgc atttcatatg
    20281 tacgtttgtc gtatctcatg gcatcaattg ttttaccatt tttggtaatc gcctgccatc
    20341 cagtttttgc ttccgctaca tcataaacgt attcaccagc atggccataa gcaactcccg
    20401 aaaaaatgtc ttctgttgct ttttgaatct tatcagcagt tgggtgatca tttttattgt
    20461 attctacatt tctatctgga attgcaactc cggtttgata tggcgcaccg ttagaagatt
    20521 gtcctgtttt ttctgtaaac ttaaatgtaa atgtcgctgt tggtgttgtt attccctctg
    20581 caatattcaa cgttttatta atcttcacaa cagcattatt ttcaccattt gctttggtta
    20641 ttgctgaatt atcatccgca aaagtcggga cacctctgaa tacggacaaa gcggttatag
    20701 ctgcaatgct tgcagtgaat agctttttaa agaatgtttt tttcatgatt atttcttttg
    20761 tttttttatt ttcttttttc attatgatca tcaaaagcct cgtcttctaa tgactgttac
    20821 aactttgcct tgagtatcct gtatagggat ggttccaaaa gcacgactgt ctacagccgt
    20881 tgttcgattg tccccgagga caaaaagttg tcctgctggg actttcatcg ggaaggttgc
    20941 cccttcctta tagagcagcg tttctttgta gatatcttgc tcttgttgag gagaaccgtt
    21001 gatgatgaga ccattttcgt ttatatcgat tgtacttcct tcggttgcta tgacacgcgc
    21061 cactctttcc ttacctttat aactatagac tagcaaatca ccaatcgaat agcgtttatc
    21121 caacctataa tagacgacca agtcgccatc ttttaaggcg ggcttcatgc catcgtcatt
    21181 gtatcttaat aatccaaaga cgaaaaagta aaggatggct agtacaaatc caaccatcag
    21241 aagtttactg actaagtaaa ggatatctcc ccagacactc ggcggagact tatcccgatt
    21301 gacttgtgtt actggtttct tatgtttctt tttaagcagc atcacttcct cctattcacc
    21361 acaaggtgac caagtaagat gataaagaag atccctccta gaggaagaac gactccaagc
    21421 acgagagtgc tactcaagaa gtcaattccc gtttcaggta cgctgttttt attattcgtg
    21481 actgtcgcag acttatttgc atcaagagtt ccttcttttt tatcatagga cacctgatac
    21541 ccacgagacg aacttgcttc ttcttcgata ctataacgag catttagagg aaggtcgaga
    21601 atcttgatgg ttttatcttt ctttagatca actgtcgcct taccattggt gaattgtagc
    21661 gttgtttttt gattatttac tattgcacta tacgatccat tcaatggttt attttgcgca
    21721 tcctttacgt taatggtaat cttaaaggat tgcagaagat tagcaaaagc acctgtaact
    21781 ttttttgtga ctgaaagtgt cggaagtctg tggttggtga cgattgtttg aacatcctct
    21841 gacaaagtcc cctcttgttt ttcataggtg acatgatatc ccgttctaga gttctctact
    21901 tcttccacct tatagtgggt atcacgaggc agataactga gggcagtttc ctgactattt
    21961 ttgagagaaa tctgtgcttt cccgttagtc actgttgtat tcccaaactt gccgttaatc
    22021 ggcttgccat ccttatctga aaaggtaaca ttaattgtga aagatttatt aggtgattgt
    22081 tcaccttcta ggattttctt tacggtgaga cttggaagct gtcggtaaat aactttgaag
    22141 gatggtttct ctttcgtacc tgtcttttga acttcgactg tgtatcccgg agcgtctggt
    22201 gctttcagtt cgtagtcata tcccttagct acctttgcaa aactagtatt ttcagtaaaa
    22261 ctattcgttt gaactggact agtgaccttt cgataggaat cctttatact tccatctttc
    22321 ttttggttta agtttaattc gacactaggt ggacgatttg ctgatggatt tgattttcca
    22381 tccacacctt tccactcaat ctctacagga accgtcaatg gatctgacgg cttgaaagtg
    22441 ggcttctcag aatagtcttt tattttggta cgtgaggtcc caataccata ggaataggca
    22501 acttttgccc ccttgttgga gtagagttta gtagcatcgg cttcttcggc atcatcacta
    22561 gtaagtgtct tatcacccgc aatcgcatca aatgcctctt ggctagaggt gacagtaaac
    22621 ttgagaacgt acttggcgtc atcctccaaa gtgtaactcg gattaaactt ggctgttact
    22681 tccactaatc cctgttcgtt cttcttagtt tcaatcgtaa cttgattgtc attcagttct
    22741 tgctcgttac catccttgat tttgacgaca cgaaggtgtg aagcatcacc aggtaacagc
    22801 tgcacgtact tggacaagac atctgatata gttacatggt gtacgcctag aggtagaatt
    22861 ttatctgtga tttctttgaa tttttgttgc aagtcatttt cattattggc agaaaatatt
    22921 tcgttgggga ttccagaatt ataaccactg atatatcctt taagagatgt gatactatcg
    22981 tttgaaccta tataatatct gaatttgatt gaataaaagc catctatttt tggagctatt
    23041 aaactagata tattcagcat tcctctttct aaaccagagg taaaccaata tggagtatca
    23101 atcatacgtc ccacatttgg attagaataa tagttatata ttgttctccc actattagac
    23161 tcgtaataca tattagcttc gccatctgac agcagaataa ctactttttt tgccccagcc
    23221 cttgcacttt gcaacaatct ttgagcttca gttaacccag catttatatt tgtcccagtg
    23281 ccaatgcccg cgtccatacc ataagaacga ctagagtcag caattgttaa cgaactaaca
    23341 atattttttg agctattaga atccgtgctc caagatacta aagttttagc atcatcatat
    23401 gaagagatat ttgaatacca accagctcaa tttggccaag ttccatattg ataactcaga
    23461 taatattcat tataataacg gtcattatac tgattatcaa tctttccggc aaaaccaact
    23521 atagacagac ggttttggga attagaaaga attgtatcaa tgagcccctg acgaccattc
    23581 acacctttca gtgtatgttt tagtgcttca atcctactaa tgtatcgtct actagagtaa
    23641 gaccacacat ctcgctcttc catactccct gaaagatcgg caaccaagac tacatccaag
    23701 ggttctgact gggtctccgt tcccagttta gatgtgatat ccagtgacaa ctcatactgg
    23761 tctttttgcc ctgatatcgg agtaatcgtt ttgtgcagag ttgtctgagg ttcagttatt
    23821 gaatctgcac gtaaagaatt aattggtgca cacaaaaaaa gaatagcgag aaagataact
    23881 aaactactgg taaaatattg aatcttctta aaatcatatt gaacgttcat gctatctccc
    23941 ttgctagaat atgttttaat ctgatactat ataaatctga gacttccata agataattgt
    24001 ataataatat taacttcgtg tcaattaatg tgtttgaaaa catgttatta taaattacaa
    24061 aaaagtatga ttcttctttg tttttcaact ttttctgtgg taaaatggaa aaaatagtaa
    24121 aaaatttcat aaaataaaga tgcatatatt tattctaatc gctcgaaaat ttcttttgaa
    24181 agtcttctga atttgaatta ttatattttt tcaaaattca taaaatttct caaatcatat
    24241 ttttccgaag ttttgtattc tattttatga taaaaaagga gtttttttat gacttatcca
    24301 aatctcttgg accgcttctt aacctacgtt aaggtcaaca cgcgctctga tgaacactct
    24361 actactactc caagtacaca gagtcaggtt gacttcgcaa caaatgtcct aattcctgaa
    24421 atgaaacgtg ttggactgca aaatgtttac tatctaccga atggttttgc tattggaacc
    24481 ttgccagcca acgatccgtc tttaacacgt aagattggtt ttatatcgca catggatact
    24541 gctgatttta atgctgaagg agtcaatcca caggtaattg aaaactacga tggtggtgtg
    24601 attgaactag ggaattctgg tttcaaactc gatccagccg acttcaagag tcttgaaaaa
    24661 tatccaagac aaacactcat cacaacagat ggaacaacct tgctaggtgc tgatgacaag
    24721 tcaggaattg ctgaaattat gacagccatt gaatatctaa ctgctcatcc tgaaattaag
    24781 cactgtgaga ttcgtgttgg ttttggtcca gatgaagaaa tcggtgttgg tgccaataaa
    24841 tttgatgcag aagattttga tgtggatttt gcctacactg ttgatggtgg tccactaggt
    24901 gaacttcagt acgagacttt ctcagccgct ggtgctgaat tgcatttcca aggccgtaat
    24961 gtccaccctg gtactgccaa agggcagatg gtcaatgccc ttcagctagc aattgatttt
    25021 cataatcaac ttccagaaaa tgaccgacct gagttaactg aaggttacca aggtttttac
    25081 catctaatgg atgtgacagg tagtgttgag gaggtgcgtg caagctacat cattcgtgat
    25141 tttgaaaaag atgcctttga agcgcgtaaa gcatccatgc aatctatcgc tgataagatg
    25201 aatgaagaac ttgggagcga ccgtgtcact ctcaacttga cagaccagta ctacaatatg
    25261 aaagaagtca ttgaaaaaga tatgactcca attaccattg ctaaagccgt tatggaagat
    25321 ctaggtatca cgcctattat cgaaccaatc cggggtggaa cagacggctc taagatttcc
    25381 tttatgggaa tcccaactcc gaatatcttt gcaggtggcg aaaatatgca cggacgtttt
    25441 gaatacgtta gccttcagac tatggaacgt gcagttgata ccatcattgg catcgtagct
    25501 tataaaggct aaaaagacga ggtggctcag ctacttcgcc tttcttttta ttctactggt
    25561 ttttcttgat ttccagtagt tgtagaagat tctgttgttt cattttctga agttgattca
    25621 gcaggtttag aatctcttgt attgcttggt ttgttttcgt cgctagcagt ttcaatgtta
    25681 gattctgcag ttgcgtttgg ttggttctca gcactggtgt tatcactatt tgcttcagca
    25741 tttcttgctg gacttgtttc ttcacttgcg ctaacttttg actggatttg atgattcaaa
    25801 actagaatag cttttgtcga ttcaagtaaa gctgttttgt ctttactatt agcagaaagt
    25861 tgatctaata atgcatccac cttatcaaag tccgcatcag atccattatt actttctaaa
    25921 taagagtgaa gcgacatgag aatatcgtag agtttttgat agagtacaag tgtctgagaa
    25981 tcttgctcag cattttcctt ttcttgttga agggcgctag cgatacgagt caaaacatct
    26041 tttacctgac tgtttacttc atccaagtct gcatcagcct tgtttgtggc agcttttaga
    26101 ttttctactt cttctgccaa ggattgtctg attccttctt catggatttg ttccaagagt
    26161 tgatttgcct tgttcaaaag actttctact tcttccttgc tatctgtcgc agattattgg
    26221 ttgctatcta ccatgtactc ctaaaacagg agagttataa tccaagatta caaggcctta
    26281 cagaaataag aaatccagat aagacaatgt tcgtccaaga cgctattcgc ttcgcacagc
    26341 agcacggatt caatatgctt taattttaaa gtttaggtgt caagacctct ttttagtgtg
    26401 cccaaaattt agagaagtaa tcaatcaact aacttttatt tttttcaaac tttcagtaaa
    26461 ctgacctaaa gctaactcaa tctgtctttg tagatgcttc tgctatcagc tagaagttga
    26521 tctacttttg ccaagactgc cttctcatca aaagttccag gttgatagtt ggattgcagg
    26581 gatggaatct tgtttttcaa agccgcttca tatcccttag tttgaacctt gatgtagtga
    26641 ttgtggtcgc catgaggaat cacaaaacct tctgaatctt cacttataat tcgattggca
    26701 tcaaaaacca taaccatctt cttcctcatg gtggacatgt agtgacggat tacttaatac
    26761 agaactagaa gaacttccta cctcttccgt gttagagtgt gatgggggat tgttaagaga
    26821 tgacttagga atatagtgat agtgttcccc atgtcttact atataagcat cacctatatc
    26881 tctgacaata tcattagggt taaagacata tgtggctgct aattcacctg ccgacaagtc
    26941 actctcagga atgaaatgat agtgaccacc atgtggtact attagtagat tgaaatagaa
    27001 tatgagcaaa ttgataaggg gattttaaag taatttctaa caatgattta gaaactatga
    27061 tgtgctattc taaattcaac tcactatata taaccatcat cggtagtata acgtccctgt
    27121 aattttgcta cagatacttc tgcactagct cctttatcgt ctttaccatg ttcttgtttt
    27181 tggcgattga tttcatcttt tgttcgtaca ttttctgcat gagcttgatc tttaaggtaa
    27241 acataatact ttccatctac cttaataata tatcctccct taacctaact gacaatatct
    27301 tgatctttcg gctgatagtt gggggctttc attaatggct cttcactaaa gagcgcatca
    27361 aaaggaactt taccattata gtagtgataa tgatcgccat gagaagttac ataaccttga
    27421 tctgtaatct taataacaat ttgttttgct tgaattcctt ctttttgact aacctagtct
    27481 ggagtcaaat tttcagtctt cttagtgtct ttattactgt ttacatatga aacacgattt
    27541 ttatctgtat tggcctgtta gctatgttgg ttcagagcat aaacacacag acttaaggaa
    27601 aggataacaa cagatccagc tgctatatat ttctttttaa atttcataat tacctcattt
    27661 ctataattat ttatatgatg tcttcattat taaatgatta tagaaattaa ttaaccaatt
    27721 aattaactag taaatattcc acctcttttt aagttgtatg tcaagaaatt ttatatatta
    27781 ataataaaat gaaattctcc caaagtcaga gttttatttc taacttttga gagaacttca
    27841 tttttgattc agactttttc tactgctatt ccttacgcta tgagatcaga taaattcttt
    27901 tttatcactt ctccacttgg caatcttaat tcaatcgttc catccatatt gaatataaca
    27961 ctatctaagc ctaatccgta actagctgta aatttttcta atttttcttg tacaggatct
    28021 actgctggag cttcctctaa tgctggatct aacatagggt cactccccac attcccttct
    28081 ggattcaaca ttccattatc cgttgagttt tctggtttta caggtttttc gtttggtgcc
    28141 tctggtaaag aatctgctgg tttattttct gttggttggt tctcaactgt tccagtagat
    28201 acttttccat tttcagatgg tttattttca ccatttcctt gaggtgcttc tcctgtaaaa
    28261 tctgccatat tctttttaat gacttctccc gatggtaaat ataattcaat tgttccgtcc
    28321 atattaaaca agacattttc tagcttcatc ccataacttt cagcaaattt tgctactttt
    28381 tcttgtacag gatccactgt aggaacttct tctaacgttg aattactagt actattccca
    28441 gtttcagaaa gtttttcttt ttctaccttc tcactagtct ttggttcttc taccttttca
    28501 tcaagttttg agttttcttg tgctttattc cttttaaatt gtggtagaat acttggttta
    28561 tcagtttgat tttctttttc caagataggt acttccacaa tataagtcga ttgattgtcc
    28621 aaataagcat ttgccatgaa ggttacagga attttatttc cggccgttct ggttgttcct
    28681 tggtttaatt tcggaatcgg taatttgatt tcaccaactt tatagttatt ttctaaataa
    28741 gcatttccat gaaattcatc aaacactctg actaaagcat cagttccttt aggcactgca
    28801 aattgagggt tcactcttaa ataagtatcc cctgcatgga aaggatagaa aatcgtttga
    28861 ctggccattt tgtaagctaa agaggttgga actgtaaatg taccatcata acttacttct
    28921 ggataatctt ttgaagcgat agtatactta aatgtttgtc ctggtaaata aggttgatct
    28981 aattcaaagt ttgcaatatt ccctactcct tctccaaata ctttaccaga tactttctcc
    29041 aatacttttc catctggtgt tattaatttt actagcatat tgatacctaa ttttttctcc
    29101 aattcaggcg gaaaactaaa agaaacgcgt ttttgaccat tggctagagt aaagttttga
    29161 ttattaaacg tactattttt taacaaatta acaacattcg ttaattcttc tccagtataa
    29221 actttattcc cttctttttt agcaactcct tcttcgggtt taaacagttc atagttactg
    29281 tgagaatgac caattccaac cggtttatgt tcatcaatcg gatctgcatg atggtgatct
    29341 ccatgcggat aaataatcgc atttttttct ttattcacga caatactttc acgtttgaca
    29401 ccatattgtt tcataatgcc agcaattttt tcttcgattt ttttatctaa atctttcatt
    29461 tctttggcat tacttggata atcctgttca tgagatgaca aagaatctaa tccattatga
    29521 ctagttttaa cttcctctaa atgtttttgc gcagccttaa tttgctcttc tgtcaagtcc
    29581 ttcttgaaga aataatgatt gtggtctccg tgactcatga caaaacctga ttcatcttca
    29641 gcgataatac gattagcatc aaatccgtat ccatcttctt catgtttctc atgtgaagtt
    29701 cctggattga ttggaagaga tggagaaggt gttgctagac tattgtttgg aagagtcggt
    29761 tgcccaattt gatttgattt tggaatgtaa tggaaatgat caccatgtct tacaatataa
    29821 gctgtagccg tttcttcaac gatatctttt ggattaaaaa tataaccatc agatgctgaa
    29881 gagagctcct tacttgtcgt taaagaagaa ggattgcttg aaagactgcc tagactagac
    29941 actacttcat taggttttgc atttgtagaa actgtagaac cagttccact gataggcacc
    30001 attctggcaa tcttttcttc taaggcagaa agcttgctgt aaggaataaa gtggtaatgg
    30061 tcgccatgcg gaatcgcaac tccatttggt gtacgactga taatcttagc agggtcaaag
    30121 accaggccat ctgattcact gtaacgttgg gcgctaggtg aatcatagag ttccttcaaa
    30181 agactctgga gattttcaga tttatttgct ggcttgctag ttgatccttt tgctacagat
    30241 tgcgtgttat tgtcactagc tgttgaagaa tagcttaact gactcggttg catatttttt
    30301 ccagccagat gtgctttagc tgctgctaat tcactagcag ataaatcgct tttgggaatg
    30361 tagtgatagt gacctccatg aggaacgata taagcattac ccgtatcttc gataatatca
    30421 gctggattaa agacataacc atcatttgtc gtatatcgtc cctgagacct tgctacagca
    30481 acattagagt taaccttctc attatctttg acatgttctt gtttttgacg attgatttca
    30541 tctttagttc gaacattatc agcatgagct gcatctttca ggtagacata atattttcca
    30601 tcgaccttga tgatataacc acccttgact tcattgacaa tatcagcgtc tttaagttga
    30661 tagtttggat ccttcatcaa gagttcttca ctaaagaggg catcataagg aactttccca
    30721 ttatagtaat gatagtggtc accgtgtgac gttacatagc cctgatctgt aattttgatt
    30781 acaatttgct cggcctgaat tccttctttc tggctaatct ggtctggtgt caagttttca
    30841 cttttctgac ttgactggct gccatctaca taagagacac gattattgtc cttattttcc
    30901 tgcgaacgat gctggtttag tgcataggca catagactca aggatacgat aacagctgat
    30961 ccagctgcta tatatttttt actgaatttc ataaatccct ca
//
LOCUS       DAVBCV010000021        30975 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBCV010000021 DAVBCV010000000
VERSION     DAVBCV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.021"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..194)
                     /locus_tag="VQ516_000934"
     CDS             complement(<1..194)
                     /locus_tag="VQ516_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2511395.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVRE"
     gene            189..443
                     /locus_tag="VQ516_000935"
     CDS             189..443
                     /locus_tag="VQ516_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2511396.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            787..2121
                     /gene="trmFO"
                     /locus_tag="VQ516_000936"
     CDS             787..2121
                     /gene="trmFO"
                     /locus_tag="VQ516_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2511397.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2205..2942
                     /locus_tag="VQ516_000937"
     CDS             2205..2942
                     /locus_tag="VQ516_000937"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2511398.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2951..3508
                     /gene="frr"
                     /locus_tag="VQ516_000938"
     CDS             2951..3508
                     /gene="frr"
                     /locus_tag="VQ516_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2511399.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3568..4422
                     /gene="cvfB"
                     /locus_tag="VQ516_000939"
     CDS             3568..4422
                     /gene="cvfB"
                     /locus_tag="VQ516_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2511400.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4431..4646
                     /locus_tag="VQ516_000940"
     CDS             4431..4646
                     /locus_tag="VQ516_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2511401.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4732..5700
                     /locus_tag="VQ516_000941"
     CDS             4732..5700
                     /locus_tag="VQ516_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2511402.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5880..6380
                     /locus_tag="VQ516_000942"
     CDS             5880..6380
                     /locus_tag="VQ516_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511403.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6383..6643
                     /locus_tag="VQ516_000943"
                     /pseudo
     CDS             6383..6643
                     /locus_tag="VQ516_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7010..8121)
                     /gene="ald"
                     /locus_tag="VQ516_000944"
                     /pseudo
     CDS             complement(7010..8121)
                     /gene="ald"
                     /locus_tag="VQ516_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8298..8867
                     /locus_tag="VQ516_000945"
     CDS             8298..8867
                     /locus_tag="VQ516_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511404.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8935..9585
                     /locus_tag="VQ516_000946"
     CDS             8935..9585
                     /locus_tag="VQ516_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2511405.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9569..11809
                     /locus_tag="VQ516_000947"
     CDS             9569..11809
                     /locus_tag="VQ516_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2511406.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11989..12177
                     /locus_tag="VQ516_000948"
     CDS             11989..12177
                     /locus_tag="VQ516_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511407.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12209..12796
                     /locus_tag="VQ516_000949"
     CDS             12209..12796
                     /locus_tag="VQ516_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511408.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12800..13975
                     /locus_tag="VQ516_000950"
     CDS             12800..13975
                     /locus_tag="VQ516_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511409.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    14149..14280
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14296..14826
                     /gene="infC"
                     /locus_tag="VQ516_000951"
     CDS             14296..14826
                     /gene="infC"
                     /locus_tag="VQ516_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2511410.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14859..15059
                     /gene="rpmI"
                     /locus_tag="VQ516_000952"
     CDS             14859..15059
                     /gene="rpmI"
                     /locus_tag="VQ516_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2511411.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15111..15470
                     /gene="rplT"
                     /locus_tag="VQ516_000953"
     CDS             15111..15470
                     /gene="rplT"
                     /locus_tag="VQ516_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2511412.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15528..15908
                     /locus_tag="VQ516_000954"
     CDS             15528..15908
                     /locus_tag="VQ516_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2511413.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16158..16958
                     /locus_tag="VQ516_000955"
     CDS             16158..16958
                     /locus_tag="VQ516_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2511414.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            16921..17907
                     /locus_tag="VQ516_000956"
     CDS             16921..17907
                     /locus_tag="VQ516_000956"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2511415.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17949..19850
                     /locus_tag="VQ516_000957"
     CDS             17949..19850
                     /locus_tag="VQ516_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET2511416.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(19911..21566)
                     /gene="pavA"
                     /locus_tag="VQ516_000958"
     CDS             complement(19911..21566)
                     /gene="pavA"
                     /locus_tag="VQ516_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2511417.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21671..22168
                     /gene="ybeY"
                     /locus_tag="VQ516_000959"
     CDS             21671..22168
                     /gene="ybeY"
                     /locus_tag="VQ516_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2511418.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22149..22544
                     /locus_tag="VQ516_000960"
     CDS             22149..22544
                     /locus_tag="VQ516_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2511419.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22561..23460
                     /gene="era"
                     /locus_tag="VQ516_000961"
     CDS             22561..23460
                     /gene="era"
                     /locus_tag="VQ516_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2511420.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPDRFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23509..24333
                     /gene="mutM"
                     /locus_tag="VQ516_000962"
     CDS             23509..24333
                     /gene="mutM"
                     /locus_tag="VQ516_000962"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2511421.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24333..24938
                     /locus_tag="VQ516_000963"
     CDS             24333..24938
                     /locus_tag="VQ516_000963"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2511422.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24925..26124
                     /locus_tag="VQ516_000964"
     CDS             24925..26124
                     /locus_tag="VQ516_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET2511423.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26124..26273
                     /gene="rpmG"
                     /locus_tag="VQ516_000965"
     CDS             26124..26273
                     /gene="rpmG"
                     /locus_tag="VQ516_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2511424.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26313..26546
                     /gene="secG"
                     /locus_tag="VQ516_000966"
     CDS             26313..26546
                     /gene="secG"
                     /locus_tag="VQ516_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2511425.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26648..29002
                     /gene="rnr"
                     /locus_tag="VQ516_000967"
     CDS             26648..29002
                     /gene="rnr"
                     /locus_tag="VQ516_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2511426.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28965..29432
                     /gene="smpB"
                     /locus_tag="VQ516_000968"
     CDS             28965..29432
                     /gene="smpB"
                     /locus_tag="VQ516_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2511427.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29448..30308
                     /gene="tehB"
                     /locus_tag="VQ516_000969"
     CDS             29448..30308
                     /gene="tehB"
                     /locus_tag="VQ516_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2511428.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEDVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30381..>30975
                     /gene="coiA"
                     /locus_tag="VQ516_000970"
     CDS             30381..>30975
                     /gene="coiA"
                     /locus_tag="VQ516_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2511429.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQG"
BASE COUNT         9196 a         5733 c         7093 g         8953 t
ORIGIN      
        1 tattctcgca catattgaag agtggccata agaaggtctt ctaggcttaa tttaggtttt
       61 cgtccacctt ttgcgtgttt aagttgataa gctgttttta atacagctaa tatctcttca
      121 aaagtcgtgc gctgaacacc aacaagacgc ttaaatcgtg catcagttag ttgtttactt
      181 gcttcataat tcatagaact actataccat attttgtttc gcaggaagtc tattggaaag
      241 taagaaatat tggagctgag gcgattagaa gaaattgtga gcgtggtgct attttttcag
      301 gtaaaataaa acatcacgaa gattctcagt ttaaaggaga ccactatgtt gaatgttatg
      361 ctgttttaga taatacggtt atagcaagag atagaataac agtccctatc gatccgttat
      421 gtggaaaaga ttttatagag tagcatataa ttgattctta actggaatac tctctatctc
      481 tttacatcaa gaaaatgact aaacagggaa gtttgccttc ttcccttttt ttgttatact
      541 agtagaagaa aaaaatagaa agatttgtgg gagtgaaaaa gtccggggga ctttttcagc
      601 ttgagccaag aaattcgaga gcgaggaaag tccgggggac tttttcagcc tgagccaaga
      661 aattcgagag cgaagtaagt ccaatggact ttttcagcct gagccaagaa actcgaaagc
      721 tcgtaagtct gattggcttt ttcaatgtga atcttaactt cacactccca aagaggtatt
      781 agtgtcgtgt ctcaatctta tatcaatgtt atcggtgctg gtttggcagg ttctgaagca
      841 gcttaccaaa tcgcagagcg tggtattcca gttaaactat atgaaatgcg tggtgtcaag
      901 tcgactcccc agcataaaac agacaatttt gctgagttgg tttgttccaa ttctttgcgt
      961 ggggatgctt tgacaaatgc agttggtctt ctcaaggaag aaatgcgtcg cttgggttct
     1021 gttatcttgg aatctgctga ggctacacgt gttcctgcag gtggtgccct tgcagtggac
     1081 cgtgatggtt tctctcaaat ggtgaccgaa aaagttgcca accacccctt gattgaagtg
     1141 gttcgtgatg aaattacaga attgccgaca gatgttatta cggttatcgc tactggtcct
     1201 ttgacaagtg atgccttggc tgaaaagatt catgccctta atgacggtga tggtttttat
     1261 ttctacgatg cggcagcgcc tattatcgat gtcaacacta tcgatatgag caaggtctac
     1321 ctcaaatcac gttatgataa gggagaagcg gcctacctca atgcccctat gaccaagcaa
     1381 gaatttatgg atttccatga agctttggtc aatgcagaag aagcaccgct taattctttt
     1441 gaaaaagaaa agtactttga aggctgtatg cctatcgaag tcatggccaa acgtggcatt
     1501 aaaactatgc tttatggccc tatgaagcca gtcggtcttg agtacccaga cgactataca
     1561 ggacctcgtg atggagaatt taaaacacct tatgcggttg tgcaacttcg tcaggacaat
     1621 gcagctggta gcctctacaa tattgttggt ttccagaccc acctcaaatg gggagaacaa
     1681 aagcgtatct tccaaatgat tccgggtctt gaaaatgcag agtttgtccg ttatggtgtc
     1741 atgcatcgca attcttacat ggattcacca aatcttcttg agcagactta ccgttctaag
     1801 aaacaaccaa atctcttctt tgctggtcag atgacggggg tggaaggcta tgttgagtcg
     1861 gcggcttcag gcttagttgc gggaattaac gcagctcgtc tcttcaagga agaaagcgag
     1921 gctattttcc ccgagacgac agcgattgga agcttagctc attacattac ccatgccgac
     1981 agcaaacatt tccaaccaat gaatgtcaat tttgggatca tcaaggagtt ggaaggcgag
     2041 cgtatccgtg ataagaaggc tcgttatgaa aaaattgcag agcgtgccct tgccgactta
     2101 gaggaatttt tgactgtcta atttttttga aagaattgct catgatacta taaaaatctt
     2161 agaaattgtg ataaaatagg taggatgaaa gaaggagagt gaaaatggcg aatcccaagt
     2221 ataaacgtat tttaatcaag ttatcaggtg aagcccttgc cggtgaacgt ggcgtaggga
     2281 ttgatatcca aacagttcaa acaatcgcaa aagagattca agaagttcat agcttaggta
     2341 tcgaaattgc ccttgttatt ggtggaggaa atctctggcg tggagaacct gcagcagaag
     2401 caggtatgga ccgtgttcag gcagattaca ctggaatgct tgggactgtt atgaatgctc
     2461 ttgtgatggc agattcattg caacaagttg gggttgatac gcgtgtacaa acagctattg
     2521 ctatgcaaca agtggcagag ccttatgtcc gtggacgtgc ccttcgtcac cttgaaaaag
     2581 gccgtatcgt tatctttggt gctggaattg gttcaccata cttctcgaca gatacaacag
     2641 cggcccttcg tgcagctgaa atcgaagcgg atgccatcct catggctaaa aatggtgtcg
     2701 atggtgttta caatgccgat cctaagaaag ataagacagc tgttaagttt gaagaattga
     2761 cccaccgtga cgttatcaat aaaggtcttc gtatcatgga ctcaacagct tcaaccctct
     2821 caatggacaa cgacattgac ttggttgtct tcaacatgaa ccaatcaggc aacatcaaac
     2881 gtgtcgtatt tggtgaaaat atcggaacaa cagtttcaaa taatatcgaa gaaaaggaat
     2941 aagaaagaat atggctaacg caattattga aaaagctaaa gagagaatga cccagtctca
     3001 ccaatcactt gctcgtgaat ttggtggtat ccgtgctggt cgtgccaatg caagcttgct
     3061 tgaccgtata catgtagaat actatggagt tgaaactcct cttaaccaaa tcgcttcaat
     3121 tacgattcca gaagcgcgtg ttttgttggt aacaccattt gacaagtctt cattgaaaga
     3181 catcgaacgt gccttgaacg cttctgatct tggtatcaca ccagctaatg acggttctgt
     3241 gattcgcttg gttgtcccag ctcttacaga agagactcgt cgtgaccttg ctaaagaagt
     3301 gaagaaggtc ggcgaaaatg ctaaagtggc tgtccgcaat atccgtcgcg atgctatgga
     3361 cgaagctaag aaacaagaaa aagcaaaaga aatcactgaa gacgaattga agactcttga
     3421 aaaagacatt caaaaagtaa cagacgatgc tgttaaacac atcgacgata tgactgccaa
     3481 caaagagaaa gaacttttgg aagtctaaaa ataaacagaa aaactcagtt ggcattgctg
     3541 gctgagtttt attcgaaaga aggaaatatg aatacaaatc ttgcaagttt tatcgttgga
     3601 ctgatcatcg atgaaaacga ccgtttttac tttgtgcaaa aggatggtca aacctatgct
     3661 cttgctaagg aagaaggcca acatacagta ggggatacgg tcaaaggttt tgcatacacg
     3721 gatatgaagc aaaaactccg cctgacaagc ttagaagtga ctgccactca ggaccaattt
     3781 ggttggggac gtgtcacaga ggttcgtaag gacttgggtg tctttgtgga tacaggcctt
     3841 cctgacaagg aaatcgttgt gtcactcgat attctccctg agctcaagga actctggcct
     3901 aagaagggcg accaactcta catccgtctt gaagtggata agaaagaccg tatctggggt
     3961 ctcttggctt atcaagaaga cttccaacgt cttgctcgtc ctgcctacaa caacatgcag
     4021 aaccaaaact ggccagccat tgtttaccgt ctcaagctat caggaacttt tgtttaccta
     4081 ccagaaaata atatgcttgg ttttattcat cctagcgagc gttacgcaga gccacgtttg
     4141 gggcaagtat tagatgcgcg cgttattggt ttccgtgaag tggaccgcac tttgaacctc
     4201 tccctcaaac cacgctcctt tgaaatgttg gaaaacgatg ctcagatgat tttgacttat
     4261 ttggaaagca atggcggttt catgacctta aatgacaagt catctccaga cgacatcaag
     4321 gcaacctttg gcatttctaa aggtcagttc aagaaagctt taggtggtct tatgaaggct
     4381 ggtaaaatca agcaggacca gtttggtaca gagttgattt agggaggctt atgagaaaat
     4441 cattttacac ttggctcatg accgagcgca atcctaaaag taacaatccc aaagcaattt
     4501 tggcagacct cgcttttgaa gaggcagctt ttccaaaaca tacagatgat tttgatgagg
     4561 tcagtcgctt tttggaggag catgccagtt tctcttttaa cctaggagat tttgacagca
     4621 tttggcagga atatctagaa cactagcatt tattcattgg gtttgggcta gtaatttctc
     4681 catccctctg ctataataaa aagaaataaa aggattagag aggttcttta tttgaaggaa
     4741 cattcaatag acattcaact gagtcatcca gatgacctgt ttcatctttt tggttccaat
     4801 gaacgccatc ttcgtttgat ggaagaagag cttgatgttg tgattcatgc tcgtacggag
     4861 attgtccagg ttttgggaga agagtctgct tgcgaggaag cccgtcaggt tattcaagct
     4921 ctcatggcct tggtaaatcg tgggatgacc gttggtacgc cagatgtagt gacggctatt
     4981 agtatggtca aaaatgatga aattgacaag tttgtcgccc tttacgaaga agaaatcatc
     5041 aaggataata ctgggaagcc tatccgcgtc aaaaccttag gtcaaaaact ttatgtggac
     5101 agtgtcaaac agcatgatgt gacctttgga attgggccag caggtacagg gaagaccttc
     5161 cttgcagtga ccttggcagt gactgccctt aaacgtgggc aagtcaagcg aattatccta
     5221 actcgtccag cggtggaagc gggagagagt cttggatttc ttccgggtga tcttaaggag
     5281 aaggtggatc cttaccttcg tcctgtttac gatgccttgt atcaaattct tgggaaagac
     5341 caaacgactc gtctcatgga gcgtgaaatt atcgaaattg cgccccttgc ctatatgcgt
     5401 ggccggacct tggatgatgc ctttgtcatt ctcgatgagg cgcaaaacac gaccatcatg
     5461 cagatgaaga tgttcttgac gcgtttaggc tttcattcta agatgattgt caatggagat
     5521 atcagtcaga ttgacctgcc acgtaatgtc aagtccggtt tgattgatgc tcaagagaaa
     5581 ctcaagaaca tccatcagat tgactttgtt catttttcag ccaaggatgt ggttcgccat
     5641 ccagttgtcg ctcagattat ccgagcctat gaatattcta ctgaagttgc acacgactga
     5701 ttttgaggaa gttcgcctgc aaaagaatat acttttgaga gaggaaaatc cagttgtata
     5761 ggctaaaggt ttgagacaac gattaggcac gatggaaaga acttttatgt ggctgatgac
     5821 gatcagtgca tcttcctgtg tcataatcac agggcacaag aaagtaggaa tttgaaaaga
     5881 tgattgacca actatctaag tattacagtt gtaggatact aactgaaaag gatattccaa
     5941 gtattttatc tttatatgaa agtaatcctc tgtattttca gcattgtcca ccagagccaa
     6001 attttgcaac tgtaaaagag gacatgcttt gtctacctga aggtaaagct aaggctgata
     6061 agttttttgt tggattttgg aatggctctg accttgtggc tgttatggat tttgtctatg
     6121 catatcctga tgaggagact gtttttattg gtttgtttat ggttgatcaa gcctatcaga
     6181 ggaaagggat tggtagtcat attgtgacag aagcactagc ttattttgct aagaactttc
     6241 gaaaggcacg tttggcttat gttaagggaa atccgcaatc tcagcatttt tgggaaaagc
     6301 agggctttaa atcaattgga tgcgaggtta agcaagaact ctatacggtt gttatcgctg
     6361 aacagagcct agaagattag aaatggcatc aagtaagaac tatttggaat ttgttttgga
     6421 acaattatca ggattagatg atgtgactta ccgttccatg attggggagt atattcttta
     6481 cttccgcggc aagattattg gcggcattta tgacgatcgc tttttagtta aacccgtgca
     6541 agcagtctta gataagattg accaatcttc ttttgagttt ccatacaaag gtgccaaaga
     6601 aatgatttga gtggaagaac ttgataataa gatgtttcta taagacctga ttttagctat
     6661 gtataaccaa ctgccaacgc ccaaacctaa aaagaaaaag caagggtgaa cgaagtaaaa
     6721 aagaagtctg ctaaggccct gtctttgcac gggtaaaatt ttatatataa aaagaagctg
     6781 ggactaaaga gctcagcttc ctttggttta tataattgtc attacaagac gaagtggttg
     6841 ggcgaaactc tgttgacttt attcaattta gagtttctta tgcacaattg agtctggaac
     6901 gaaagtctcc agttgcaaag tatacagtac aataaaccaa cgatgtaata gctgatgaca
     6961 caaagcacag tgggtaggac ttgcgaagtc acccttttct tttcaaaatt tatactaaat
     7021 cattgatatc agtgtagtca cgattaagtc cttgagcaac tggtaggcta gtcaagtaac
     7081 cttgataagt ggtcacacct tgacgcaagc cttcatcttc agagattgct tgtgcgaatc
     7141 ctttgccagc caaagcttcg atataaggaa gagtgacatt ggttagggcg atggttgaag
     7201 tgcgggcaac cgcaccaggg atattggcaa cggcatagtg gagaacaccg tgtttttcat
     7261 agacgggttc atcgtgcgtt gtcacacggt cagctgtttc gataacgcca ccttggtcaa
     7321 cagcaacgtc aacgatacag agcctggacg catttgtttg accatctcat ctgtcaccaa
     7381 ttccggtgct tttgcaccag ggatgagaat ggctccaatc accacatcag catctctcac
     7441 acttgcttca atgttgaatg aattagatat aatagtttga atttgacttc caaagacttc
     7501 ttctagaact gagagacgct tggcactaat atctaaaata gtcacttgag caccaagacc
     7561 aagggcgatg cgggcagcat gtgtaccgac gacaccatca ccgatgatag ttacttttcc
     7621 ttttggaaca cctggtacac caccaagtag aacaccagag ccaccagctt gcttagtaag
     7681 gaagtgagct ccgatttgaa cagccatacg acctgcaacc tcactcatag gaacgaggag
     7741 cggtagttgt ccttgattgt cacgaacagt ttcataggca attcctgttg tttttgctgc
     7801 taacatagca tctgctaatt ctggagcagc ggccatgtgc aagtaggtga agagaagaag
     7861 atcgtcgcgc aagtaaccgt attcagaact taaaggttct tttactttca caaccaactc
     7921 tgctgcccaa gcttcaccag cagtagcgac aatctcagct ccttgctttt gatagtcagc
     7981 atcagtaaag ccagaaccga gaccagcatt tgtttcgata aggacacgat gaccacgact
     8041 aactaagcta tgaacacctg caggtgtgag ggcgacacgg ttttcgttat ttttaatttc
     8101 ttttgggatt ccgattaaca ttgagataac ctacctttca attgacggtc ttgttttggt
     8161 tgtcacattc cagttcataa atcaaaaatg tgacggtttc attgtatatg aaaccgcttc
     8221 aaaaatcaag aaaaacttgt catccaaatt tttttatgct agactagtga aaatcaagct
     8281 ctaatggagg gaaaagtatg gaatcaatat ttgtgaaatt tgcccagtat ccgtctatag
     8341 aaacggagcg tttattgctt agacctgtaa ctttggatga tgcggaagca atgtttgact
     8401 atgcctcgga caagggtaat acacgttaca cttttccaac caatcaaagc ttggaagaaa
     8461 ccaagaataa cattgctcag ttctacttgg ctaatccctt gggacgttgg gggattgaat
     8521 taaaagataa cggcaagttt atcggaacta ttgacttaca caagattgat cctgttctta
     8581 agaaggcagc tattggctac attatcaata aaaagtattg gaatcaagga ttaacgacag
     8641 aagccaatcg tgctgtgatt gagctagctt ttgagaagat agggatgaat aagttgactg
     8701 cccttcacga taaggctaat cccgcgtcag gaaaggtcat ggagaaatca ggcatgcgtt
     8761 tttcccatgc agaaccatat gcttgtatgg accagcatga aaaaggccga atcgtgacaa
     8821 gagttcatta tgtcttgacc aaggaagact attttgcaaa taaataagca gttgaaaaga
     8881 aatttttcga ctgttttttc ttcctcttac gaataatcta agagaggaga aaatatggaa
     8941 gcaattatcg agaaaatcaa agagtataaa atcatcgtca tctgtactgg tctgggcttg
     9001 cttgtaggag gatttttcct gctaaagcca gctccacaaa catctgtcaa agagacgaat
     9061 ttgcaggctg aagttgcagc tgtttccaag gactcatcga ccggaaagga agtgaacaag
     9121 gaagagaagg aagaaccagt tgaacaagat ctaatcacag tagatgtcaa aggtgctgtc
     9181 aaatcgccag ggatttatga cttgcctgta ggtagtcgag tcaatgatgc tgttcagaag
     9241 gctggtggct tgacagagca agcagacagc aagtcgctca atctagctca gaaagttagt
     9301 gatgaggctc tggtttacgt tcctactaag ggagaagaag cagttagtca acagactggt
     9361 tcggggacag cttcttcaac aagcaaggaa aagaaggtca atctcaacaa ggccagtctg
     9421 gaagaactca agcaggtcaa gggactggga ggaaaacgag ctcaggacat tattgaccat
     9481 cgtgaggcaa atggcaagtt caagtcagta gacgagctca agaaggtctc tggcattggt
     9541 gccaagacca tagaaaaact taaagactat gttacagtgg attaagaatt tctctattcc
     9601 cctaatttac ctgagttttc tattactatg gctttactac gccattttct cagtatccta
     9661 ttttgctttg ttgggttttg tttttctgct agtctgcctc tttatccaat ttccttggaa
     9721 atcagcaggt aaagttctag tgatttgtgg agtctttggc ttctggtttc tgtttcaaac
     9781 ttggcaacag agtcaagtga gtcaaaattt ggtggattct gttgaaaggg tacggatttt
     9841 accagacact attaaggtta acggtgacag tctgtccttt cgtggtaagt ctgatggtcg
     9901 tgctttccaa gtctattata aactccagtc cgaggaggag aaagaagcct ttcaagcttt
     9961 aactgacctt catgagatag gactagaagg gaagctttcg gagccagaag ggcagagaaa
    10021 ttttggtggc tttgactacc aaggctatct gaagactcag ggaatttacc agacactcaa
    10081 tattaaaaga atccagtcac tccaaaaggt tggcagttgg gatataggtg aaaacctgtc
    10141 cagtttacgt cgaaaggctg tggtttggat taagatgcac ttcccagacc ctatgcgcaa
    10201 ttacatgaca ggactcttgc taggacatct ggacaccgat tttgaggaga tgaatgagct
    10261 ttattccagt ttaggaatta ttcacctttt tgccttgtca ggtatgcagg tagggttttt
    10321 catggacgga tttaagaaac tacttttacg attgggcttg acacaagaaa agttgaagtg
    10381 gctgacttat cccttttccc ttatctatgc gggactaact ggattttcag catcggttat
    10441 tcgcagtctc ttgcaaaagc tactggctca acatggggtt aagggcttgg ataattttgc
    10501 cttgacggtg cttgtcctct ttattgtcat gccaaacttt ttcttgacag caggaggagt
    10561 cttgtcctgt gcttatgctt ttatcctgac catgaccagc aaagaagggg aggggctcaa
    10621 ggctgttact agtgaaagtc tagtcatctc cttgggcata ttgcccattc tatccttcta
    10681 ttttgcggaa tttcaacctt ggtctatcct tttgaccttt gtcttttcct ttctttttga
    10741 cttggtcttc ttaccgctct tgtctatctt atttgtcctt tcctttctct atccagtcat
    10801 tcagctgaac tttatctttg aatggttaga gggcattatt cgcttggtct cgcaggtggc
    10861 aagtagaccg cttgtctttg gacaacccaa tgaatggctt ttaatcctat tgttaatttc
    10921 tttggctttg gtctatgatt tgaggaaaaa cattaaaaag ctaacggtat tgtgcttatt
    10981 gattacaggg ctctttctcc tgaccaagca tccactggaa aatgaaatca ccatgctgga
    11041 tgtggggcaa ggagaaagta ttttcctacg ggatgtaact gggaaaacca ttctcataga
    11101 tgtaggtggt aaggcagaat cttataagaa aatcaaaaaa tggcaagaaa agatgacgac
    11161 cagcaatgcc cagcgaagct tgatacccta tctcaaaagt cgaggagtag ctaagattga
    11221 ccagctaatt ttgactaaca cggacaagga gcatgttgga gatttgtcag agatgaccaa
    11281 ggctttccat gtaggggaga ttctagtatc aaaagacagt ctgaaacaga aggaatttgt
    11341 ggcagaacta caggcgactc aaacaaaggt gcgtagtatg atagtagggg agaacttacc
    11401 catttttgga agtcagttag aagttctatc tccaaggaaa atgggagatg gaggacacga
    11461 tgatacccta gttctgtatg ggaaattctt ggataagcaa tttctcttca cgggaaattt
    11521 ggaggagaaa ggagagaagg acttgctgaa gcactatcca gacttgaaag taaatgtttt
    11581 gaaagctagc caacatggca ataaaaaatc atcaagtcca gcctttctag aaaaactcaa
    11641 accagagctt actcttatct cagttggaaa gagcaatcga atgaaactcc cccatcagga
    11701 aacattgaca cgactggaag gtatcaatag caaagtttat cgaactgacc agcaaggagc
    11761 tatacgtttt aaggggttgg atagttggaa aatcgaaagt gttcgatagg aaggataaat
    11821 gttgtagatt agtgaaataa actaaaaatt tgttgcataa taatgataaa aacggcataa
    11881 tgaaaacgta ttcaatattg aggatataaa atcattaaaa atcagcaaaa gttgttttag
    11941 ttagttagtt tataatctat tggtcttctt cagtccagtg tatctgctgt gacagtcact
    12001 aaaagttaca agtatgattg gaatacggtt ttggaatata gtaccaacta tcacgaccat
    12061 cagtatgctt ggattccgga atggtctcgt tattacagct attctgagta taaagttggc
    12121 ggaggctgga actacgctcg ttatgaggtc ataaactact atactggagg ttattaatcc
    12181 ttaaagagtg agaaaaggag ggctggatat gttaaatctt actcatgtta ccttaaaaac
    12241 gcgacaagtc atcttgcaag atgcggattt tacctttaaa aagggtagga tttatggcct
    12301 tcttgctatc aatggctcgg gaaagacgac actattccga gctatgagca agttgcttcc
    12361 ccttagtagt ggacacatcg cagttcctcc ttctttgttt tattatgaga gcgttgaatg
    12421 gctggatgga aacttaagtg ggatggacta ccttcgtctc ataaaaaaca tctggaagtc
    12481 agacctaaac ttgagagatg aaatcgccta ctgggaaatg gctgactata tcagtcttcc
    12541 catccgcaag tattccttag ggatgaagca acgcttggtg attgctatgt attttctcag
    12601 tcaggccaaa tgctggctca tggatgagat tacaaatggc ttagacgagt attatcgaca
    12661 gaagtttttt gataggctag cacaaatcga tagacaagaa cagctggttc ttttaagttc
    12721 ccactataag gaagagttgg ttgatatctg cgatagagta gtaaccattc atcaggggca
    12781 gatagaagag gtttagttta tgaaagatgt tagtctattt ttattgaaaa aagttttcaa
    12841 aagtcgttta aactggatta tcttagcttt atttgtatct gtactcggtg ttacctttta
    12901 tttaaatagt cagactgcaa actcacacag cttggagagc aggttggaaa gtcgcattgc
    12961 agccaacgag agggctatca atgaaaatga agagaaactc tcccaaatgt ctgataccag
    13021 ctcggaggaa taccagtttg ctaaaaataa tttagacgtg caaaaaaatc ttttgacgcg
    13081 aaagacagaa attctaactt tactaaaaga agggcgctgg aaagaagcct actatctgca
    13141 gtggaaagat gaagagaaga attatgaatt tgtatcaaat gacccgactg ctagctctgg
    13201 cttaaaaatg ggggttgacc gcgaacggaa gatttaccaa gccctgtatc ccttgaacat
    13261 aaaagcacat actttggagt ttccgaccca cgggattgat cagattgtct ggattttaga
    13321 ggttatcatc ccaagcttgt ttgtggttgc gattattttt atgctaacac aactatttgc
    13381 agaaagatat caaaatcatc tggacacagc tcacttatat cctgtttcaa aagtgacatt
    13441 tgcaatatcc tctcttggag ttggagtggg atatgtaact gtgctgttta tcggaatctg
    13501 tggcttttct tttctagtgg gaagtctgat aagtggtttt ggacagttag attatcccta
    13561 cccaatttat agcttagtga atcaagaagt aactattggg aaaatacaag atgtattatt
    13621 tcctggcttg ctcttagctt tcttagcctt tatcgtcatt gtggaagttg tgtacttgat
    13681 tgcttacttt ttcaagcaaa aaatgcctgt cctctttctt tcactcattg ggattgttgg
    13741 cttattgttt ggcatccaaa caattcagcc tcttcaaagg attgcacatc tgattccctt
    13801 tacttacttg cgttcagtgg agattttatc tggaagatta cctaagcaga ttgataatgt
    13861 cgatctaaat tggagcatgg gaatggtctt acttccttgc ctgattatct ttttgctatt
    13921 gggaattcta tttattgaaa gatggggaag ttcacagaaa aaaagaattt tttaatagat
    13981 tctagctttc ctataggtag ggaaaataag taaaaactaa catagagagg gaatcaactt
    14041 gattctctct ttttgattcg aaaaccaaac caaaatacaa acacaaactt ttcaaaaaat
    14101 aactttttat cttgacaaga gctagaaaac ttggtatcat ataaaagttg agaaaagcag
    14161 aagtgagagc ttctcgcctt gtgacattaa gttgcctggc cctacggatg aaaagtttcg
    14221 aagaaacgct atcataacgt gcgggcttgt atatttacga gtccgctatt gtttttctct
    14281 aataaaacaa aagaggtgaa aaccatagca aagcaagact tattcatcaa tgatgagatt
    14341 cgtgtacgtg aagttcgctt gattggtctt gaaggagaac agctaggcat caagccactc
    14401 agtgaagcgc aagctttggc tgataacgct aatgttgacc tagtattgat tcaaccccaa
    14461 gccaaaccgc ctgttgcaaa aattatggac tacggtaagt tcaaatttga gtaccagaag
    14521 aagcaaaaag aacaacgtaa aaaacaaagc gttgttactg tgaaagaagt tcgtctaagt
    14581 ccaactattg acaagggtga ctttgataca aaacttcgta atgcacgtaa gttccttgaa
    14641 aaaggaaata aagttaaggt atctattcgc tttaagggtc gtatgattac ccataaagag
    14701 attggtgcaa aagttttagc cgagtttgct gaagcaactc aagatattgc catcatcgaa
    14761 caacgtgcta aaatggatgg acgtcaaatg ttcatgcaat tggcgccagc gactgacaaa
    14821 aaataattgt cagaaagtta aataaaggag aaaaaattat gccaaaacaa aaaacacacc
    14881 gcgcatcagc taaacgtttc aaacgtacag gttctggtgg acttaaacgt ttccgtgctt
    14941 acacttctca ccgtttccac ggaaaaacta agaaacaacg tcgtcatctt cgtaaagcat
    15001 ctatggtgca ttcaggagat tacaaacgta tcaaagcaat gcttactcgc ttgaaataat
    15061 agcttgactg taagtaaaca attctaggaa atatttggag gaaataaaac atggcacgtg
    15121 ttaaaggtgg cgttgtatca cgcaaacgtc gtaaacgtat tcttaaatta gcaaaaggtt
    15181 actatggagc taaacacatc ttgttccgta ctgcaaaaga acaagtaatg aactcttact
    15241 actatgcata ccgtgaccgt cgtcagaaaa aacgtgactt ccgcaaattg tggatcactc
    15301 gtatcaatgc ggcagctcgt atgaacggac tttcatactc acaattgatg catggtttga
    15361 aattggctga gatcgaagtt aaccgtaaaa tgcttgctga cttggctgtt aacgatgcag
    15421 tagctttcac agctcttgca gatgcagcta aagcaaaact tggtaaataa caactgatga
    15481 gactggaaca ggattgtcca gtctttttaa aaataaagga gaaaaatatg gcttcaaaaa
    15541 tgctacacac ttgcttacga gtagaaaatc ttgaaaaatc aatcgcattt tatcaagatg
    15601 cttttggttt taaagaattg cgtcgcagag attttccaga ccatgccttc acgattgtct
    15661 atctaggtct tgagggtgat gactatgagt tggagttgac ttataactac gatcacggtc
    15721 cgtatgtggt tggtgatggg tttgcccata tcgccctcag tacacctgat cttgaggcac
    15781 ttcatcaaga acacagtgca aaaggctatg aagtgactga gccaaatggt ctaccaggaa
    15841 ctacaccaaa ctattacttt gtcaaagacc ctgatggata taaggtcgaa gtcattcgtg
    15901 aaaaataatt tcgtgcaagt taagtagaat attcgttttt tacttgactt tttttagctg
    15961 aaagagtata ctaataaaaa ttcaaccttt aaggggagtc cagagagact cacaaggtgt
    16021 cagataaaag aatagtgcaa ttttctagag gagacttttt gagtgtgctc tcttgtgttg
    16081 tacgatttta actgaggcct tgcactagca aggtcttttc tttatctggt ccccttaaaa
    16141 tttaaggagg aaaagttatg aatcccacat gtaagaagcg tttgggtgtc attcggttgg
    16201 aaaccatgaa ggtggttgca caagaggaaa tcgcgccagc aatctttgaa ttagtcctag
    16261 aaggagaaat ggttgaagcc atgcgagcag gccaatttct tcatctgcgt gtaccagacg
    16321 atgcccatct tttacgtcgt cctatttcaa tttcgtctat tgacaaggca aacaagcagt
    16381 gtcacctcat ttatcggatt gagggagctg ggactgcaat tttttcaacc ttaagtcagg
    16441 gagacactct tgatgtgatg gggcctcagg gaaatggttt tgacttgtct gaccttgatg
    16501 agcagaatca ggttctcctt gttggtggtg ggattggtgt tccacccttg cttgaggtgg
    16561 ccaaggaatt gcatgaacgt ggagtgaaag tagtgacagt cctcggtttt gctaataagg
    16621 atgctgttat tttgaaaacg gaattggctc agtatggtca ggtctttgta acgacagatg
    16681 atggttctta tggcatcaag ggaaatgttt ccgttgttat caatgattta gatagtcagt
    16741 ttgatgctgt ttactcgtgt ggggctccag gaatgatgaa gtatatcaat caaacctttg
    16801 atgatcaccc aagagcctat ttatctctgg aatctcgtat ggcttgtggg atgggagctt
    16861 gctatgcctg tgttctaaaa gtaccagaaa gcgagacggt caaccaacgc gtctgtgaag
    16921 atggtcctgt tttccgcaca ggaacagttg tattataagg agaaaattat gactacaaat
    16981 cgattacaag tgtctctacc tggtttggat ttgaaaaatc cgattattcc agcatcaggt
    17041 tgttttggct ttggacaaga gtatgccaag tactatgatt taaacctttt aggttctatt
    17101 atgatcaagg cgacaaccct tgaaccacgt tttgggaacc caactccaag agtagcagag
    17161 acgcctgctg gtatgctcaa tgcaattggc ttgcaaaatc ctggtttaga ggttgttttg
    17221 gctgaaaagc taccttggct ggaaagagaa tatccaaatc ttcctattat tgccaatgta
    17281 gctggttttt caaaacaaga gtatgcagct gtttctcatg ggatttccaa ggcaactaat
    17341 gtaaaagcta tcgagctcaa tatttcttgt cccaatgttg accactgtaa tcatggactt
    17401 ttgattggtc aagatccaga tttggcttat gatgtggtga aagcagctgt ggaagcctca
    17461 gaagtgccag tttatgtcaa attaaccccg agtgtgaccg atatcgttac tgtcgcaaaa
    17521 gctgcagaag atgcgggagc aagtggcttg accatgatca atactctggt tggaatgcgc
    17581 tttgacctca aaaccagaaa accaatcttg gccaatggaa caggtggaat gtcaggtcca
    17641 gcagtctttc cagtagccct caaactcatc cgccaagtag cccaaacaac agacctgcct
    17701 atcattggaa tggggggagt ggattcggct gaagctgccc tagaaatgta tctggctggg
    17761 gcatctgcta tcggagttgg aacagctaac tttaccaatc cttatgcctg ccctgacatc
    17821 atcgaaaatt taccaaaagt catggataaa tacggtatta gcagtctgga agaactccgt
    17881 caggaagtaa aagagtctct gaggtaaact gcaatcaatc tgttcttgat tttttattag
    17941 tttgtaatat gaatttagga gaattttggt acaataaaat aaataagaac agaggaagaa
    18001 ggttaatgaa gaaagtaaga tttatttttt tagctctgct atttttctta gctagtccag
    18061 agggtgcaat ggctagtgat ggtacttggc aaggaaaaca gtatctgaaa gaagatggca
    18121 gtcaagcagc gaatgagtgg gtttttgata ctcattatca atcttggttc tatataaaag
    18181 cagatgctaa ctatgctgaa aatgaatggc taaagcaagg tgacgactat ttttacctca
    18241 aatctggtgg ctatatggcc aaatcagaat gggtagaaga caagggagcc ttttattatc
    18301 ttgaccaaga tggaaagatg aaaagaaatg cttgggtagg aacttcctat gttggtgcaa
    18361 caggtgccaa agtaatagaa gactgggtct atgattctca atacgatgct tggttttata
    18421 tcaaagcaga tggacagcac gcagagaaag aatggctcca aattaaaggg aaggactatt
    18481 atttcaaatc cggtggttat ctactaacaa gtcagtggat taatcaagct tatgtgaatg
    18541 ctagtggtgc caaagtacag caaggttggc tttttgacaa acaataccaa tcttggtttt
    18601 acataaaaga aaatggaaac tatgctgata aagaatggat tttcgagaat ggtcactatt
    18661 attatctaaa atctggtggc tacatggcag ccaatgaatg gatttgggat aaggaatctt
    18721 ggttttatct caaatttgat gggaaaatag ctgaaaaaga atgggtctac gattctcata
    18781 gtcaagcctg gtactacttc aaatccggtg gttacatgac agccaatgaa tggatttggg
    18841 ataaggaatc ttggttttac ctcaagtctg atggcaaaat ggctgaaaaa gaatgggtct
    18901 acgattctca tagtcaagcc tggtactact tcaaatctgg tggttacatg gcgaaaaatg
    18961 agacagtaga tggttatcag cttggaagcg atggtaaatg gcttggagga aaagctacaa
    19021 atgaaaatgc tgcttactat caagtagtgc ctgttacagc caatgtttat gattcagatg
    19081 gtgaaaagct ttcctatata tcgcaaggta gtgtcgtatg gctagataag gatagaaaaa
    19141 gtgatgacaa gcgcttggct attactattt ctggtttgtc aggctatatg aaaacagaag
    19201 atttacaagc gctagatgct agtaaggact ttatccctta ttatgagagt gatggccacc
    19261 gtttttatca ctatgtggct cagaatgcta gtatcccagt agcttctcat ctttctgata
    19321 tggaagtagg caagaaatat tattcggcag atggcctgca ttttgatggt tttaagcttg
    19381 agaatccctt ccttttcaaa gatttaacag aggctacaaa ctacagtgct gaagaattgg
    19441 ataaggtatt tagtttgcta aacattaaca atagcctttt ggagaacaag ggcgctactt
    19501 ttaaggaagc cgaagaacat taccatatca atgctcttta tctccttgcc catagtgccc
    19561 tagaaagtaa ctggggaaga agtaaaattg ccaaagataa gaataatttc tttggcatta
    19621 cagcctatga tacgacccct tacctttctg ctaagacatt tgatgatgtg gataagggaa
    19681 ttttaggtgc aaccaagtgg attaaggaaa attatatcga taggggaaga actttccttg
    19741 gaaacaaggc ttctggtatg aatgtggaat atgcttcaga cccttattgg ggcgaaaaaa
    19801 ttgctagtgt gatgatgaaa atcaatgaga agctaggtgg caaagattag tactataagt
    19861 gaatatgatt tgagtgaata gtaagttaaa aatcctgatt tcaagtaaaa tcaggatttt
    19921 ttcatggatg caattttttt ggagtctggt gtgacgcgga gggtcttttg tcctgtgtaa
    19981 gtgacaaagc cgggttttcc accagttggt ttattgagtt ttttgacttc aatcatatct
    20041 acctgcacca gattcgacag gcgcccttga gagaagtagg cagctaactc tgctgcgtct
    20101 gtcttgactg catcagatgg gtcaagattt cctgagatga caacatggct tccaggaatg
    20161 tccttagcat ggaaccaaag ttcctccttg cgggccattt taaaggtcaa ttcctcattt
    20221 tgaagattgt ttcgtccgac atagatgatg gttttgccat cgcttgctag atattgttct
    20281 agttttttgc gtttctggat tttctcccgt tgtcttctgc ggataaaacc tgtttgaatc
    20341 aattcttcac ggatttcagc gatttcttcc agtccagctt ggttgaggac ggtttctaca
    20401 ctttccagat agagaatagt ggctttggtt tcttcaatca aatcagtcaa gtatttgaca
    20461 gcttctttga gtttctgata ccgtttaaaa tagcgttggg cattctggtt gggagtcaga
    20521 gccttatcaa gcgcaatcat gataggttgg ttggtatagt agttgtctag gataacctgg
    20581 tcttggtcgt taggcacttg gtggaggaag gttgtcagca attctccttt ttgacgaaat
    20641 tcttcagcgt tgtctgtcgc cagtaactct ttttcctgtt ttttgagttt gtgtcggttt
    20701 ttctgaagtt cattttcaac acgacgaatc agttcactgg cctgctgttt gacgcggtcg
    20761 cgctcagcct tatccttata gtaggtgtcc aacaaatcag aaagatttgc aagaggctct
    20821 cccacctgat ttgcaaaagg aactggactg aaggaagtct cagtcaagca tggcttggtt
    20881 tcttgattga aaaaatttcg gaaagcggaa agtttttcac taaccagtat cctttccaat
    20941 tcatttgccg tatcgcgtcc cagaccttga aagaggcttt gaagattttt tgctgttagt
    21001 tcttgggttt gcaggatttc aaagagcttt tcatccttga tagtaaaagg attgagagat
    21061 tttgtacttg gcggagcgat ataggtcgat cctggaagta aggtgcggta gctattttgt
    21121 gaaaagccga cgtgtttgat aacttcgagg attttatgac tgcttttatc gaccagtaga
    21181 atattactgt gtttccccat aatttcgata atcaaggtag cctggatatg gtctccaatc
    21241 tcgtttttat tggaaactgt aatttccaca atacggtcat tttccacttg ctcaatcgac
    21301 tcaatcaggg ccccctgcaa atactttctc aaaaccatga taaaggtaga aggttgggct
    21361 ggattttcaa aagtcgtttg ggtcagctga atgcgtccaa aaactggatg ggcagaaagg
    21421 agcaggcgat ggctttggcg attgctgcgg atttgcaaga ccaactcttg ttcaaaaggc
    21481 tgattgattt tctggatgcg accattcact aattcgcttc gcaattcctc aactatgtgg
    21541 tgtaaaaaaa atccgtcaaa tgacatcgtt ctctccttgt gattgtattc catagtatta
    21601 tatcaaaaag gtagaataaa atcatggaaa tgtggtataa taaagccaag taaagagaaa
    21661 cgagaagcac atgtatattg aaatggtaga tgaaactggt caagtttcaa aagaaatgtt
    21721 gcaacaaacc caagaaattt tggaatttgc agcccaaaaa ttaggaaaag aagacaagga
    21781 gatggcagtc acttttgtga ccaatgagcg tagtcatgaa cttaatctgg ggtaccgtga
    21841 caccgaccgt ccgacagatg tcatcagcct tgagtataaa ccagaattgg aaattgcctt
    21901 tgacgaagag gatttgcttg aaaatccaga attggcagag atgatgtctg agtttgatgc
    21961 ctatattggg gaattgttca tctctatcga taaggctcat gagcaggccg aagaatatgg
    22021 tcacagcttt gagcgtgaga tgggcttctt ggcagtacac ggctttttac atattaacgg
    22081 ctatgatcac tatactccgg aagaagaagc ggagatgttc ggtttacaag aagaaatttt
    22141 gacagcctat ggactcacaa gacaataaac gaaaatggaa aaatcgtgac ctgatatcca
    22201 gtttagaatt tgccatcaca gggattttta ctgctatcaa ggaagaacgc aatatgcgaa
    22261 aacatgcagt gacggctcta gtggtcatcc ttgcaggttt tggttttcag gtgtcacgaa
    22321 tcgaatggct ctttctccta ttgagcattt tcttggtggt agcctttgaa attatcaatt
    22381 ctgctatcga aaatgtggtg gatttggcca gtcactatca cttttccatg ctggctaaaa
    22441 atgccaagga tatggcggct ggcgcggtat tagtggtttc tctttttgca gccttaacag
    22501 gcgcattgat ttttctccca cgaatctggg atttattatt ttaaacagta agaggaaatt
    22561 atgactttta aatcaggctt tgtagccatt ttaggacgtc ccaatgttgg gaagtcaacc
    22621 tttttaaatc acgttatggg gcaaaagatt gccatcatga gtgacaaggc gcagacaacg
    22681 cgcaataaaa tcatgggaat ttacacgact gataaggagc aaattgtctt tatcgacaca
    22741 ccagggattc acaaacctaa aacagctctc ggagatttca tggttgagtc tgcctacagt
    22801 acccttcgcg aagtggacac tgttcttttc atggtgcctg ctgatgaagc gcgtggtaag
    22861 ggggacgata tgattatcga gcgtctcaag gctgccaagg ttcctgtgat tttggtggtg
    22921 aataaaatcg ataaggtcca tccagaccag ctcttgtctc agattgatga cttccgtaat
    22981 caaatggact ttaaggaaat tgttccaatc tcagcccttc agggaaataa cgtgtctcgt
    23041 ctagtggata ttttgagtga aaatctggat gaaggtttcc aatatttccc gtctgatcaa
    23101 atcacagacc atccagaccg tttcttagtt tcagaaatgg ttcgcgagaa agtcttgcac
    23161 ctaactcgtg aagagattcc gcattctgta gcagtagttg ttgactctat gaaacgagac
    23221 gaagagacag acaaggttca catccgtgca accatcatgg tcgagcgcga tagccaaaaa
    23281 gggattatca tcggtaaagg tggcgctatg cttaagaaaa tcggtagcat ggcccgtcgt
    23341 gatatcgaac tcatgctagg agacaaggtc ttcctagaaa cctgggtcaa ggtcaagaaa
    23401 aactggcgcg ataaaaagct agatttggct gactttggct ataatgaaag agaatactaa
    23461 gtagaggtgg gctcatgcct gcttcttgtt tttacagaag gaggacttat gcctgaatta
    23521 cctgaggttg aaaccgtttg tcgttgctta gaaaaattga ttataggaaa gaagatttcg
    23581 agtatagaaa ttcgctaccc caagatgatt aagacggatt tggaagagtt tcaaagggaa
    23641 ttgcctagtc agattatcga gtcaatggga cgtcgtggaa aatatttgct tttttatctg
    23701 acagacaagg ttttgatttc ccatttgcgg atggagggca agtattttta ctatccagac
    23761 caaggacctg aacgcaagca tgcccatgtt ttctttcatt ttgaagatgg tggcacgctt
    23821 gtttatgagg atgttcgcaa gtttggaacc atggaactct tggtgcctga ccttttagac
    23881 gcctacttta tttctaaaaa attaggtcct gaaccaagcg aacaagactt tgatttacag
    23941 gtctttcaat ctgcccttgc caagtccaaa aagcctatca aatcccatct cctagaccag
    24001 accttggtag ctggacttgg caatatctat gtggatgagg ttctctggcg agctcaggtt
    24061 catccagcta gatcttccca gactttgaca gcagaagaag cgacagctat tcatgaccaa
    24121 accattgctg ttttgagcca ggctgttgaa aaaggcggtt ccaccattcg gacctatacc
    24181 aatgcctttg gggaagatgg aagcatgcag gattttcatc aggtctatga taagactggt
    24241 caaggatgtg tacgctgtgg taccatcatt gagaaaattc aactaggtgg acgtggaacc
    24301 cacttttgtc caaactgtca aaggagggac tgatgggaaa aatcatcgga atcactgggg
    24361 gaattgcctc tggtaagtca actgtgacaa attttctaag acagcaaggc tttcaagtag
    24421 tggatgccga cgcagtcgtc caccaactac agaaacctgg tggtcgtctg tttgaggctc
    24481 tagtacagca cttttggcaa gaaatcattc ttgaaaacgg agaactcaat cgccctctcc
    24541 tagctagtct catcttttca aatcctgatg aacgagaatg gtctaagcaa attcaagggg
    24601 agattatccg tgaggaactg gctactttga gagaacagtt ggctcagaca gaagagattt
    24661 tcttcatgga tattccccta ctttttgagc aggactacag cgattggttt gctgagactt
    24721 ggttggtcta tgtggaccga gatgcccaag tagaacgctt aatgaaaagg gaccagttgt
    24781 ccaaagatga agctgagtct cgtctggcag cccagtggcc tttagaaaaa aagaaagatt
    24841 tggccagcca ggttcttgat aataatggca atcagaacca gcttcttaat caagtgcata
    24901 tccttcttga gggaggtagg caagatgaca gagattaact ggaaggataa tctgcgcatt
    24961 gcctggtttg gtaattttct gacaggagcc agtatttctt tggttgtacc ttttatgcct
    25021 atcttcgtgg aaaatctagg tgtagggagt gagcaagtcg ctttttatgc aggcttagca
    25081 atttctgtct ctgctatttc cgcggcgctc ttttctccta tttggggtat tcttgctgac
    25141 aaatacggcc gaaaacccat gatgattcgg gcaggtcttg ctatgactat cactatggga
    25201 ggcttggcct ttgtcccaaa tatctattgg ttaatctttc ttcgtttact aaacggtgta
    25261 tttgcaggtt ttgttcctaa tgcaacggca ctgatagcca gtcaggttcc aaaggagaaa
    25321 tcaggctctg ccttaggtac tttgtctaca ggcgtagttg caggtactct aactggtccc
    25381 tttattggtg gctttatcgc agaattattt ggcattcgta cagttttctt actggttggt
    25441 agttttctat ttttagctgc tattttgact atttgcttta tcaaggaaga ttttcaacca
    25501 gtagccaagg aaaaggctat tccaacaaag gaattattta cctcggttaa atatccctat
    25561 cttttgctca atctcttttt aaccagtttt gtcatccaat tttcagctca atcgattggc
    25621 cctattttgg ctctttatgt acgcgattta gggcagacag agaatcttct ttttgtctct
    25681 ggtttgattg tgtccagtat gggcttttcc agcatgatga gtgcaggagt catgggcaag
    25741 ctaggtgaca aggtgggcaa tcatcgtctc ttggttgtcg cccagtttta ttcagtcatc
    25801 atctatctcc tctgtgccaa tgcctctagc ccccttcaac taggactcta tcgtttcctc
    25861 tttggattgg gaaccggtgc cttgattccc ggggttaatg ccctactcag caaaatgact
    25921 cccaaagccg gcatttcgag ggtctttgcc ttcaatcagg tattctttta tctgggaggt
    25981 gttgttggtc ccatggcagg ttctgcagta gcaggtcaat ttggctacca tgctgtcttt
    26041 tatgcgacga gcctttgtgt tgcctttagt tgtctcttta acctgattca atttcgaaca
    26101 ttattaaaag taaaggaaat ctagtgcgag taaaaattaa tctcaaatgc tcctcttgtg
    26161 gcagtatcaa ttacctaacc agtaaaaatt caaaaaccca tccagacaag attgaggttt
    26221 taaagtattg tcccaaggaa agaaaagtaa ccctacatct tgaatctaag tagattttat
    26281 ggtaaaataa tagggatttt aaggagtttg atatgtataa cctattatta accattttat
    26341 tagtattatc tgttgtgatt gtgattgcaa ttttcatgca accaaccaaa aaccaatcca
    26401 gcaatgtatt tgatgccagt tcaggtgatt tgtttgaacg tagtaaagct cgcggttttg
    26461 aagctgtaat gcagcgtttg acagggattt tagtcttttt ctggctagcc attgccttag
    26521 cattgacggt attatcaagt agataagaaa ataatgggca ggactaggtc tttgcctctt
    26581 tttattttta aaggatgttt gagaaggttt tacagtaaaa gaaaattaaa aaatctagaa
    26641 agaaaatatg aaagatagaa taaaagaata tttacaagac aagggaaagg tgactgttaa
    26701 tgatttggct caggctttgg gaaaagacag ttccaaggat tttcgtgagt tgattaaaac
    26761 cttgtcctta atggaaagaa agcaccaaat tcgttttgaa gaagatggta gtctgacatt
    26821 agaaattaag aaaaaacatg agattaccct caaggggatt tttcatgccc ataaaaatgg
    26881 ctttggcttt gttagtctgg aaggcgagga ggacgacctt tttgtaggga aaaatgatgt
    26941 caactatgct attgatggtg ataccgtcga ggtagtgatt aagaaagtcg ctgaccgcaa
    27001 taagggaaca gcagcagaag ccaaaattat tgatatccta gaacacagtt tgacaacagt
    27061 tgtcgggcaa atcgttctgg atcaggaaaa acctaagtat gctggctata ttcgttcaaa
    27121 aaatcagaaa atcagtcaac cgatttatgt taagaaacca gccctaaaat tagaaggaac
    27181 agaagttctc aaagtcttta tcgataaata cccaagcaag aaacatgatt tctttgtcgc
    27241 gagtgttctc gatgtagtgg gacactcaac ggatgtcgga attgatgttc ttgaggtctt
    27301 ggaatcaatg gacattgtat ccgagtttcc agaagctgtt gttaaggaag cagaaagtgt
    27361 gcctgatgct ccgtctcaaa aggatatgga aggtcgtctg gatctaagag atgaaattac
    27421 ctttaccatt gacggtgcgg atgccaagga cttggacgat gcagtgcata tcaaggctct
    27481 gaaaaatggc aatctggagc ttggggttca catcgcagat gtttcttatt atgtgaccga
    27541 ggggtctgcc cttgacaagg aagcccttaa ccgtgcgact tctgtttacg tgacagaccg
    27601 agtggtgcca atgcttccag aacgactatc aaatggcatc tgctctctca atccccaagt
    27661 tgaccgcctg acccagtctg ctattatgga gattgataaa catggtcgtg tggtcaacta
    27721 taccattaca caaacagtta tcaagaccag ttttcgtatg acctatagcg atgtcaatga
    27781 tatcctagct ggcgatgaag aaaagagaaa agaatatcat aaaattgtat caagtatcga
    27841 actcatggcc aagcttcatg aaactttaga aaacatgcgt gtgaaacgtg gagctctcaa
    27901 ttttgatacc aatgaagcga agattttagt ggataaacaa ggtaagcctg ttgatatcgt
    27961 tcttcggcag cgtggtattg ccgagcggat gattgagtct tttatgttga tggctaatga
    28021 aacagttgcc gaacatttca gcaagttgga tttgcctttt atctatcgaa ttcacgagga
    28081 gcctaaggct gaaaaggttc agaagtttat tgattatgct tcgagttttg gcttgcgcat
    28141 ttatggaact gccagtgaga ttagtcagga ggcacttcaa gacatcatgc gtgctgttga
    28201 gggagaacct tatgcagatg tattgtccat gatgcttctt cgctctatgc agcaggctcg
    28261 ttattcggag cacaatcacg gccactatgg actagctgct gactattata ctcactttac
    28321 cagtccaatt cgtcgttatc cagaccttct tgttcaccgt atgattcggg attacggccg
    28381 ttctaaggaa atagcagagc attttgaaca agtgattcca gagattgcga cccagtcttc
    28441 caaccgtgaa cgtcgtgcca tagaagctga gcgtgaagtc gaagccatga aaaaggctga
    28501 gtatatggaa gaatacgtgg gtgaagagta tgatgcagtt gtatcaagta ttgtcaaatt
    28561 cggtctcttt gtcgaattgc caaacacagt tgaaggcttg attcacatca ctaatctacc
    28621 tgaattttat catttcaatg agcgtgattt gactcttcgt ggagaaaaat caggtatcac
    28681 tttccgagtg ggtcagcaga tccgtatccg tgttgaaaga gcggataaaa tgactggaga
    28741 gattgatttt tcattcgtac ctagtgagtt tgatgtgatt gaaaaaggct tgaaacagtc
    28801 tagtcatagt ggcagagggc gtggttcaaa tcgtcgttcg gataagaagg aagacaagag
    28861 aaaatcagga cgctcaaatg ataagcgtaa gcattcacaa aaagacaaga agaaaaaagg
    28921 aaagaaacct ttttacaagg aagtagctaa gaaaggagcc aagcatggca aagggcgagg
    28981 gaaaggtcgt cgcacaaaat aaaaaggcac gccacgacta tacaatcgta gatacgctag
    29041 aggcagggat ggtcctgact ggaactgaaa tcaagagtgt acgagctgct cgaattaatc
    29101 tcaaggatgg ctttgctcaa gtgaaaaatg gagaagtttg gctgagcaat gttcatatcg
    29161 cgccttacga agagggcaat atctggaacc aggaaccaga acgtcgtcgt aaactcctgc
    29221 tccataaaaa gcaaattcaa aaattggaac aagagaccaa agggacagga atgaccttag
    29281 ttccccttaa ggtctatata aaagatggct acgctaagct tcttttagga cttgccaaag
    29341 ggaagcatga ctatgacaaa cgggagtcta tcaaacgtcg tgagcaaaat cgagatatcg
    29401 cgcgtgtgat gaaagctgtt aatcagcgat aaaaagagga attgaaaatg gaaaaattag
    29461 ttgcctataa acgcatgcct ttgtggaata aacaaacaat gcctgaagat gttcagcaaa
    29521 agcacaatac aaaagttggg acttggggga aaattactgt cttgaaggga gctctcaagt
    29581 ttattgaatt gacagaagaa ggggaagttc tagctgaaca cctctttgaa gcaggggcag
    29641 acaatccaat ggcccaacct caagcctggc accgagtgga agctgccaca gatgatgtgg
    29701 aatggtactt ggaattttat tgtaaacctg aggattattt tgctaaaaaa tacaatacca
    29761 atcctgttca ttcagaggtc ctagaggcca tgcagacagt gaaacaaggg aaagctttgg
    29821 atttgggttg tggtcagggg cgtaattctc tttttctagc ccagcaagat tttgatgtga
    29881 cggctgtaga tcaaaatgga ctagctcttg aaatcttgca aagcattgtg gagcaggaag
    29941 atttggacat gcctgttggc ctttacgata tcaattcagc tagcattgaa caagaatatg
    30001 attttatcgt ttcaacagtt gttctcatgt ttctacaagc ggaccgcatt ccagctatta
    30061 ttcaaaatat gcaggagaaa accagtgttg gtggttacaa ccttatcgtt tgtgccatgg
    30121 acacggagga ttatccttgc tcggttaatt tcccattcac ctttaaagaa ggagaactgg
    30181 cagactatta caaggattgg gaattggtta agtacaatga aaatccaggc catttgcacc
    30241 gtcgcgatga gaatggcaat cgtattcaac tacgctttgc gaccttacta gctaagaaaa
    30301 tcaagtaaac acacatgaag attaggaatt ttcctgatct tttttctttt ttacgaatga
    30361 tatagaaaag gagggaattc atgtttgttg cgagagatgc taggggagaa ttggtaaatg
    30421 tgttagagga taaacttgag aagcaagcat acacctgccc agcttgtgga ggccagctcc
    30481 atttgcgtca aggaccaagt gtacggacgc attttgccca taaatcctta aaagactgtg
    30541 attttttctt tgaaaatgaa agtccagaac acctggccaa taaggaatcc ctctatcact
    30601 ggttgaaaaa agagacaaag gttcaattag agtacccgct ttcagaactt aaacagattg
    30661 cggatgtatt tgtaaatggc aatctagctc tagaagttca gtgtagtccc ttgcctcaga
    30721 aagtccttaa agagcgaagt gagggatatc gtagtcaggg ttaccaagta ctgtggttgc
    30781 tgggtcaaaa actgtggctc aaggagcgtt tgactcgtct acagcaaggt tttctttatt
    30841 tcagtcaaaa catgggcttt tatgtttggg aattagacaa ggaaaaacaa gttttaagac
    30901 tcaaatacct gatttaccag gatctccgcg gtaaactcca ttatcaaatc aaggaatttt
    30961 cctatggtca aggta
//
LOCUS       DAVBCV010000022        30074 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBCV010000022 DAVBCV010000000
VERSION     DAVBCV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.022"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            51..1100
                     /locus_tag="VQ516_000971"
     CDS             51..1100
                     /locus_tag="VQ516_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2511430.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAVHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(1200..5042)
                     /gene="spuA"
                     /locus_tag="VQ516_000972"
     CDS             complement(1200..5042)
                     /gene="spuA"
                     /locus_tag="VQ516_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2511431.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPEHQDPAPEARPDSTKPDTKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            5459..5872
                     /gene="rpsL"
                     /locus_tag="VQ516_000973"
     CDS             5459..5872
                     /gene="rpsL"
                     /locus_tag="VQ516_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2511432.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            5892..6362
                     /gene="rpsG"
                     /locus_tag="VQ516_000974"
     CDS             5892..6362
                     /gene="rpsG"
                     /locus_tag="VQ516_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2511433.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            6787..8868
                     /gene="fusA"
                     /locus_tag="VQ516_000975"
     CDS             6787..8868
                     /gene="fusA"
                     /locus_tag="VQ516_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2511434.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            8972..13363
                     /locus_tag="VQ516_000976"
     CDS             8972..13363
                     /locus_tag="VQ516_000976"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2511435.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            13465..13728
                     /locus_tag="VQ516_000977"
     CDS             13465..13728
                     /locus_tag="VQ516_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2511436.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            13721..13999
                     /locus_tag="VQ516_000978"
     CDS             13721..13999
                     /locus_tag="VQ516_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2511437.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            14020..14172
                     /locus_tag="VQ516_000979"
     CDS             14020..14172
                     /locus_tag="VQ516_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511438.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            14194..15435
                     /locus_tag="VQ516_000980"
     CDS             14194..15435
                     /locus_tag="VQ516_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2511439.1"
                     /translation="MVLPNFKENLGKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFKNAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            15445..15675
                     /locus_tag="VQ516_000981"
     CDS             15445..15675
                     /locus_tag="VQ516_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2511440.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            15898..16620
                     /locus_tag="VQ516_000982"
     CDS             15898..16620
                     /locus_tag="VQ516_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2511441.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            16837..18171
                     /gene="pepC"
                     /locus_tag="VQ516_000983"
     CDS             16837..18171
                     /gene="pepC"
                     /locus_tag="VQ516_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2511442.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(18227..19138)
                     /locus_tag="VQ516_000984"
     CDS             complement(18227..19138)
                     /locus_tag="VQ516_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2511443.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(19162..19965)
                     /locus_tag="VQ516_000985"
     CDS             complement(19162..19965)
                     /locus_tag="VQ516_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2511444.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(19993..20982)
                     /gene="manX"
                     /locus_tag="VQ516_000986"
     CDS             complement(19993..20982)
                     /gene="manX"
                     /locus_tag="VQ516_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2511445.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(21215..22234)
                     /gene="adhP"
                     /locus_tag="VQ516_000987"
     CDS             complement(21215..22234)
                     /gene="adhP"
                     /locus_tag="VQ516_000987"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2511446.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(22573..23385)
                     /locus_tag="VQ516_000988"
     CDS             complement(22573..23385)
                     /locus_tag="VQ516_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2511447.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            23575..24993
                     /locus_tag="VQ516_000989"
     CDS             23575..24993
                     /locus_tag="VQ516_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2511448.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            25046..25753
                     /locus_tag="VQ516_000990"
     CDS             25046..25753
                     /locus_tag="VQ516_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2511449.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            25848..26822
                     /gene="folP"
                     /locus_tag="VQ516_000991"
     CDS             25848..26822
                     /gene="folP"
                     /locus_tag="VQ516_000991"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2511450.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGITPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            26824..28146
                     /locus_tag="VQ516_000992"
     CDS             26824..28146
                     /locus_tag="VQ516_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2511451.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            28127..28681
                     /gene="folE"
                     /locus_tag="VQ516_000993"
     CDS             28127..28681
                     /gene="folE"
                     /locus_tag="VQ516_000993"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2511452.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            28724..29536
                     /gene="folK"
                     /locus_tag="VQ516_000994"
     CDS             28724..29536
                     /gene="folK"
                     /locus_tag="VQ516_000994"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2511453.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFEFYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPTLKQPIRNLYDALKK"
     gene            complement(29581..29898)
                     /locus_tag="VQ516_000995"
     CDS             complement(29581..29898)
                     /locus_tag="VQ516_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2511454.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMYGRETILLPEEN
                     DLF"
BASE COUNT         8527 a         5840 c         7006 g         8701 t
ORIGIN      
        1 ttagaataat ttttcaaatt gaaggacaga gatagacaag gagaatcaca atggtagaat
       61 tgggaatttc aacatttggg gaaataacgg agcttgaagg gactggacaa acttacagtc
      121 atgctgaacg cattcgccag ttggtggcag agattgagct ggctgacaag gttggtttgg
      181 acgtatatgg gattggcgag caccatcggg cggattttgc agtatcagcc ccagagattg
      241 tcctggccgc tggggcagtc aataccaaga aaatccgttt gaccagtgca gtcagcattc
      301 tgtcaagcat ggatccgatt cgtttgttcc aacagtatgc cactatcgat gctttgtcaa
      361 atggacgagc ggagattatg gctggaaggg gctctttcac ggaatctttc cctctgtttg
      421 gttatgactt gaaagactac gactctcttt ttgatgagaa attagacttg cttcagttag
      481 tcaatgaaaa gaccaagtta gactggcaag gtcgattgac tcaaacgatt gctggtaaag
      541 aagtttatcc tcgtccagtt caggacaaat tgcccttgtg gatagctaca ggtggtcatg
      601 tcgaatcaac agtgaagatt gctcaggctg gtctaccgat tgtatatgct attattggtg
      661 gtaatccacg ttattttaaa aagttgattc aggcttatcg tgagattgga agcgaagcgg
      721 tccatgctga caaagacttg aaagtgggag cccattcttg ggggtggatt gcggaagatg
      781 gtgagcaggc ggtgaaagat tatttccatc cgactaagca agtggtggat gctatttcca
      841 aagaccgtcc gcactggcag gaattgcgtt atgagcaata tttggagcag gtagggtcaa
      901 atggcgccat gtttgttggc aatccagatc aggtggccga aaaattgatt cgcatgatag
      961 aggatttagg attggaccgc ttcatgcttc atctaccgct tggttctatg cctcatgacc
     1021 aggttctgag agctattgaa ctcttcggaa cacaagttgc acccaaagta cgagcttatt
     1081 ttgctatgaa agaggcttaa taaaaaatcc taatcaagtt tattaggaca acaaatattg
     1141 tacgaaaatt aatcccccga gctataatgc ttgggggatt tttctatagg agggctagtt
     1201 tagttctctt ttttattttt tagtaagaaa ccgagaccaa ggagcgcaag gaggctgatt
     1261 cctgcaaata ggagtttgtt ttcgttttta attcctgtat ttggaagttc agcttgtcta
     1321 tctggggttg caggtatatt tttcttgcta gagttttcta ccgattcgtt tcgaaccgct
     1381 tcttttacag atgatgcttg tgcttcttgt gctggttgtt cagcttgtga atcagctgta
     1441 gcttggctag gtttattttc cgcatcagct actttggtat ctggtttagt agaatcaggt
     1501 ctagcttctg gagctggatc ttgatgttca gggtgagaag cttcattttg atgttcaggc
     1561 ttggaagggg ttgagtctgg tttctcttct gcagttgact catggctagt tccattttga
     1621 gagactcgaa gaacagtagc tgtaagggca ttcaatttca agcctttttc agtccattca
     1681 agtcctttcg ggttggcaat tccgactgat cctgcttggt tttcatctgc caaaacttcc
     1741 gcatttctta gatgtgcaaa ggcagttccc aaattaaatt cgcgagcttt ttcatccgca
     1801 ttgacaaaga ctgcgtagat atcgccgttt ggagcagtga tttgatagcc aatcactaca
     1861 tcctcttttt ccacaccatt ttggcctggg acagtgatga ggtggacacg gtctttgata
     1921 tcttgaagac tcttaagtcg gaaggcatct gtagattgac gaagggcaat caaacctttc
     1981 atatagtcac ggctcttgac attttcagga taagctttac catctgtagc cttagtccag
     2041 tcaaacttgt tgactgcatc actagaatcg taagagtcat ggatgaagta aggatagtca
     2101 aatgggttgc cgtccttatc acgcaacaag tgagatttgt ttggaacctt atcctctgct
     2161 actggagtct tgtaggctgg gtcacggaat tgtttagtac gtccatattc ctgaccggag
     2221 tggataaatg gagttccttg agctgtcaag accatgagat ttccaagtcg taaacgacgg
     2281 tggatttcag catagttctc agccttgctt gggtcttttt tgatagactg ggcaatgatg
     2341 tcaaagaggg tcaagttatc atgggctgcg atgtattgga tgacatctcc agggctgtca
     2401 gcttcaaagt tagttggttg agcaatgaga tttttaaaga tggtgttgac atcacgcttg
     2461 ccacctgtga taaaggcagg ttgaccttcg tttggataac cagatttgag gttgttacgg
     2521 atgtcatctg aaaagacagc gacagtatcg gtatgtttca tccaatcttg gtcagcagct
     2581 ttagtaggca tgttttcatc accggcatag gttctccaac cttcaccaag catgatgagg
     2641 tttggattga gggcgcgtgc agccttgtaa gcttcttcga tagaagcggc gtcatggtct
     2701 cccatcatat cgaaacggaa gccatccact ttgtaggtat caactaggta tttgatagag
     2761 tcaattagga gccgtttggt catatggtgg gttgtcccca agcgtccacc accaaagcta
     2821 gttcgaggtg tgccatcggc atccataaag tggtagtagt ttggttccaa atcttcaaag
     2881 agatcgactt tggctgtgtg gttataaacg acatctagga tagctcccat accacgtttg
     2941 tggatttcgt tgatgaggtt tttaaattct gcgattcgtt tttctggatt cttaggatcg
     3001 cttgagtaca taccagtcaa ggagaagtag ttttgagggt catatcccca gttgtagttg
     3061 ctgttgcttg aagcgtagtc agacaagcgt tcatggtttt tcaattcatt gacaaagtag
     3121 taagacaaga ctggaaggag ctggatatgg gttacaccca agtctttgag atagtctagt
     3181 ttttcaatga aggcttcaaa agtcccaaat ggtttggtca agtcttttgc aatggcagga
     3241 tctgaagtga aatcacgcac atgagcttcg tagataacgg cgtcttcacg agtcttgaaa
     3301 ttgtgaatct taccataagt caagtcttga ggtccgagtt tagctggatc tacaaaggcg
     3361 gctttagcca ctttatgggc atcgtcaatc ttggaatcgt cgctattcca agcagcaaga
     3421 gatttagcgt aaggatcgag tgcaagaaca gttttacctt gacgctcgat ttggtattga
     3481 taatagtagc cagtgaaatc tgtgattccg agtttgtttg tgctgtctag agtttgtttc
     3541 caagttcctc tttccccttt ttcaagagcg acagttccaa ctactttgtc agggtcattc
     3601 ttgtcgtaga caacaacaga aaccttatca gcacttggtg accaaagggt caaatcaact
     3661 tgttttcctt cttcttttag gtcagctccc agtttgccat catagctgta tgtctcatct
     3721 ttcaggcgcc agcttgtttt ggtagtgaat tggtcggaat tgtagctaac agtataagga
     3781 tgttttgtgt cagagaaatc tccgctgtag gtcactttct taccagcttc atcgattgca
     3841 acatcggtaa tagttacctt gtttcctagg tgattagtga tgttggagtg tttgaggata
     3901 tcttcttttt tagcaccgac aagtgttgaa aagctacttt caatgctaga agtgcctacg
     3961 tgttgggctc ctgtcatacg gatatcatgg acatagtatg gatttgtgta aatcgattca
     4021 tcatcgtctt ttaggaaaat ttggctatga tttttcaaat ctgtgaactt ataattttct
     4081 ttacggattt tcacgtcgtc tccttgtttg ctctcatcta gtaataaaaa tccaaattct
     4141 cttgcggctt cattaagagg aatgtcgata tagcggccat atttgcctgt agccgtaaag
     4201 tctgttccgt caggccattg agcgctactt ggatttttca catctcccca gtaccagaga
     4261 gatttcttgt catagttgcc atctgtgcgg tagtagttga cgcgaacagt tcctgcaggc
     4321 tgtggctcgt aagagaaaac cttgtaatct tggtctaacc aagcttcgtt catttttgga
     4381 actagttttt ctacagattt atcgccggtt agattttttc cagctgtatt gttgatgagg
     4441 aagctaattt tcttggcttg ttctcccttt aatttgacat ctaggtaata gccgtagtca
     4501 tctttcttgg catccttgaa ggacaaagct ccgtttggcc agttttcaga tggtttttca
     4561 acatcgtccc aagtccatag tccttgagca tccttgtttt cttcaggaag ttttttgaca
     4621 tggatacgga agtagttatc ttcgatgggc tcttctgcct tagtttcagt tgttactgga
     4681 ctagtagaag tagttggctc acttgaacga tcttgtccag tttgtggtgc tggatcggct
     4741 gggcttgcag caggtttttc ttctgttttc tctagggaag cgtttgcatt tccaggtgaa
     4801 gaaatatcac tttcgttctt accagagcca gctgctgtat cagtaagagg ttgggcaagg
     4861 gtggttgtat ttctgttgtt gggttcgtta gtggttggtg tactttcgtc ggatgagata
     4921 gttggtgtag ccatggcaag caagacaagg cttgcgccga taaggacaga accagttcca
     4981 tttttgaggg aacggatgct gtagaccatt tttttctcag tgtgagatgg tgtttttctc
     5041 ataatgattc tccaatcaat aaatttctat atcagtatag catgtagtaa aacaatatgc
     5101 aagcgttttc tttgatttgt gaaagaaatt cttacttttg agaagttttc cgtactattt
     5161 tatatatcag tataaatgac ggagataaaa atgtcacgta agccaagccg tgttattagg
     5221 aagatgatgt gttctcagct atttttattc agaaattgat ctctaacatt accaataaaa
     5281 aagaaaaata tcttgacaag caagggaaaa ttttgttaca ataatagacg gtacttttta
     5341 cttttggtct ctcaagagtg tacagggacg tgctgacaaa tgttgcaaaa gtacacacag
     5401 atgatatctg tcaccaagtg tatcatcacc aaaaataaaa aaacacagga gaatgtagat
     5461 gcctacaatt aaccaattgg ttcgcaaacc gcgtaaatca aaagtagaaa aatctaaatc
     5521 accagctttg aacgttggtt acaatagtca taaaaaagtt caaacaaacg tttcttcacc
     5581 acaaaaacgt ggtgttgcaa ctcgtgttgg aacaatgaca cctaaaaaac ctaactcagc
     5641 ccttcgtaaa ttcgctcgtg tacgtttgag caaccttatc gaagttactg cctacatccc
     5701 aggtatcgga cacaacttgc aagagcacag cgtggtgctt cttcgcggtg gacgtgtaaa
     5761 agaccttcca ggggtacgtt accatatcgt ccgtggtgca cttgatactg caggtgttaa
     5821 cgatcgtaaa caaggccgtt ctaaatacgg tactaaacgt ccaaaagcat aaggaaaggg
     5881 gataaagaga aatgagtcgt aaaaatagag ctccaaaacg tgacgtattg ccagatccgc
     5941 tatacaattc acaactagtt actcgtctta tcaaccgcgt tatgcttgat ggtaaacgtg
     6001 gtactgctgc ttcaatcgtt tacggtgctt ttgagcaaat caaagaagct actggcaacg
     6061 atgcacttga agtatttgaa acagctatgg aaaacatcat gcctgtactt gaagtacgtg
     6121 cacgtcgtgt tggtggttct aactaccaag tcccagttga agttcgtcca gaacgtcgta
     6181 caacacttgg acttcgttgg ttggtaacaa tcgctcgtct tcgtggtgaa cacacaatgc
     6241 aagaccgtct tgcaaaagaa atcttggatg ctgctaacaa cactggtgca gcagttaaga
     6301 aacgtgaaga tactcaccgt atggctgaag ctaaccgtgc attcgcacac ttccgttggt
     6361 aagataggat gcgaaagcgt taagaaagtc ccagagaaaa tagggaatcg aagcaggttg
     6421 cggttgcaac caatgagatt catctttttc tccagacttt tagcttgagc tcaactaaat
     6481 catgatgcta ggaacggtaa ggatgcaagg taaaaatagg aaactgacgc agtattcgac
     6541 gaatacaagg agttttatct ttttcacgca gcatcccgtt ccagctcaca tcggctaact
     6601 aactttagcc cgggttcaaa ttagctaaat cgattagtat tagctataac tcagcttacc
     6661 atctcgtaag ttgaaaccaa caatagcatg aaaacattga gaacgggtag gtcctgccta
     6721 tccgttttta ttaaaatcgt gttataatag aatagaaatc aaaaataaat aggagaaaca
     6781 aacctcatgg cacgcgaatt ttcacttgaa aaaactcgta atatcggtat catggctcac
     6841 gtcgatgccg gtaaaacaac aactactgag cgtattcttt actacactgg taaaatccac
     6901 aaaatcggtg aaactcacga aggtgcgtca caaatggact ggatggagca agagcaagaa
     6961 cgtggtatca cgatcacatc tgctgcgacg acagctcaat ggaacaacca ccgcgtaaac
     7021 atcatcgaca caccaggaca cgtggacttc acaatcgaag tacaacgttc tcttcgtgta
     7081 ttggatggtg cggttaccgt tcttgactca caatcaggtg ttgagcctca aactgaaaca
     7141 gtttggcgtc aagcaactga gtacggagtt ccacgtatcg tatttgccaa caaaatggac
     7201 aaaatcggtg ctgacttcct ttactctgta agcacacttc acgatcgtct tcaagcaaat
     7261 gcacacccaa tccaattgcc aatcggttct gaagatgact tccgtggtat cattgacttg
     7321 atcaagatga aagctgaaat ctatactaac gaccttggta cggatatcct tgaagaagac
     7381 atcccagctg aataccttga ccaagctcaa gaataccgtg aaaaattgat tgaagcagtt
     7441 gctgaaactg acgaagaatt gatgatgaaa tacctcgaag gtgaagaaat cactaacgaa
     7501 gaattgaaag ctggtatccg taaagcgact atcaacgttg aattcttccc agtattgtgt
     7561 ggttcagcct tcaaaaacaa aggtgttcaa ttgatgcttg atgcggttat cgactacctt
     7621 ccaagcccac ttgacatccc agcaatcaaa ggtattaacc cagatacaga cgctgaagaa
     7681 attcgtccag catctgacga agagccattt gcagctcttg ccttcaagat catgactgac
     7741 ccattcgtag gtcgtttgac attcttccgt gtttactcag gtgttcttca atcaggttca
     7801 tacgtattga atacttctaa aggtaaacgt gaacgtatcg gacgtatcct tcaaatgcac
     7861 gctaacagcc gtcaagaaat cgacactgtt tactcaggtg atatcgctgc tgccgttggt
     7921 ttgaaagata ctacaactgg tgactcattg acagatgaaa aagctaaaat catccttgag
     7981 tcaatcaacg ttccagaacc agttatccaa ttgatggttg agccaaaatc taaagctgac
     8041 caagacaaga tgggtatcgc ccttcaaaaa ttggctgaag aagatccaac attccgcgtt
     8101 gaaacaaacg ttgaaactgg tgaaacagtt atctcaggta tgggtgaact tcaccttgac
     8161 gtccttgttg atcgtatgcg tcgtgagttc aaagttgaag cgaacgtagg tgctcctcaa
     8221 gtatcttacc gtgaaacatt ccgcgcttct actcaagcac gtggattctt caaacgtcag
     8281 tctggtggta aaggtcaatt cggtgatgta tggattgaat ttactccaaa cgaagaaggt
     8341 aaaggattcg aattcgaaaa cgcaatcgtc ggtggtgtgg ttcctcgtga atttatccca
     8401 gcggttgaaa aaggtttggt agaatctatg gctaacggtg ttcttgcagg ttacccaatg
     8461 gttgacgtta aagctaagct ttatgatggt tcatatcacg atgtcgactc atctgaaact
     8521 gccttcaaga ttgcggcttc actttccctt aaagaagctg ctaaatcagc acaaccagct
     8581 atccttgaac caatgatgct tgtaacaatc actgttccag aagaaaacct tggtgatgtt
     8641 atgggtcacg taactgctcg tcgtggacgt gtagatggta tggaagcaca cggtaacagc
     8701 caaatcgttc gtgcttacgt tccacttgct gaaatgttcg gttacgcaac agttcttcgt
     8761 tctgcatctc aaggacgtgg tacattcatg atggtatttg accactacga agatgtacct
     8821 aagtcagtac aagaagaaat tattaagaaa aataaaggtg aagactaatc cgtcctcact
     8881 ctagaaggaa gtcacttagt ggcttccttt tgtctttaga aaatacctct aaatatggta
     8941 aaatagtaga agaataatgt gaggaaaatg aatgtcaaat agttttgaaa ttttgatgaa
     9001 tcaattgggg atgcctgctg aaatgagaca ggctcctgct ttagcacagg ccaatattga
     9061 gcgagttgtg gttcataaaa ttagtaaggt atgggagttt catttcgtat tttctaatat
     9121 tttaccgatt gaaatctttt tagaattaaa gaaaggtttg agcgaagaat tttctaagac
     9181 aggcaataaa gctgtttttg aaattaaggc tcggtctcaa gaattttcaa atcagctctt
     9241 gcagtcctac tatagggagg ctttctctga aggtccatgt gctagtcaag gttttaagtc
     9301 cctttatcaa aatttgcaag ttcgtgctga gggtaatcag ctatttattg aaggatctga
     9361 agcgattgat aaggaacatt ttaagaagaa tcatcttcct aatttagcca aacaacttga
     9421 aaagtttggt tttccaactt ttaactgtca agtcgagaag aatgatgtcc tgacccaaga
     9481 gcaggaagag gcctttcatg ctgaaaatga gcagattgtt caagctgcca atgaggaagc
     9541 gctccgtgct atggaacaac tggagcagat ggcacctcct ccagcggaag agaaaccagc
     9601 ctttgatttt caagcgaaaa aagctgcagc taaacccaag ctggataagg cggagattac
     9661 tcctatgatc gaagtgacga cagaggaaaa tcgtctggta tttgaagggg ttgtttttga
     9721 tgtggagcaa aaagtgacta gaacaggtcg tgttttaatc aactttaaaa tgacggacta
     9781 tacttcaagt ttttctatgc aaaagtgggt taaaaacgag gaagaggccc agaagtttga
     9841 cctcatcaag aagaattctt ggctccgagt tcgagggaat gtggagatga ataacttcac
     9901 acgcgatttg actatgaacg tacaggatct gcaggaagtt gttcactatg agcggaagga
     9961 tttgatgcca gaaggtgagc gtcgggttga gtttcatgct catactaaca tgtcgactat
    10021 ggatgctttg ccagaggtcg aagagattgt tgcaacagct gctaagtggg gacacaaggc
    10081 ggttgctatc acggaccatg ggaatgtcca gtcctttcca catggctata aggcggctaa
    10141 gaaagcggga atccagctga tctatgggat ggaagccaat atcgtggagg accgtgtccc
    10201 tatcgtctat aacgaagtgg agatggactt gtcagaagca acctacgtgg tctttgacgt
    10261 ggaaacgacg ggactttcag ctatctataa tgacttgatt caggttgcgg cttctaagat
    10321 gtacaagggg aatgttattg ctgaatttga tgaatttatc aatcctgggc atcccttgtc
    10381 agcctttact acagagttaa ctggaattac agatgatcat gtcaaaaatg ccaaaccact
    10441 agaacaagtt ttgcaagaat tccaagaatt ttgcaaggat acggtcctag ttgcccacaa
    10501 tgctaccttt gacgttggct ttatgaatgc taattatgag cggcatgatc ttccaaagat
    10561 tagtcagcca gttattgata cgctggagtt tgctagaaac ctctatcctg agtataaacg
    10621 ccatggtttg gggcctttga ccaagcgttt tggtgtggcc ttggaacatc accacatggc
    10681 caactacgat gcggaagcga ctggtcgtct gcttttcatc tttatcaaag aggtagcaga
    10741 aaaacatggt gtgaccgatt tagctagact caacattgat ctaatcagtc cagattctta
    10801 caaaaaagct cggatcaagc atgcgaccat ctatgtcaag aatcaggtag gtctaaaaaa
    10861 tatctttaag ctggtttcct tgtctaatac caagtatttt gaaggagtgc cacggattcc
    10921 gagaacggtt ctagatgccc atcgagaggg cttgatttta ggttcagcct gttcagaggg
    10981 tgaagttttt gacgtggtcg tttctcaagg tgtggatgcg gcggttgagg tggccaagta
    11041 ttatgatttt atcgaggtca tgccaccggc tatctatgca cccttgattg ccaaagagca
    11101 ggtcaaggat atggaggaac tccagaccat tatcaagagt ttgatagagg ttggagaccg
    11161 ccttggcaag cctgttctgg ctacgggaaa tgttcactat atcgaaccgg aagaagagat
    11221 ttatcgtgaa attatcgtcc gtagtttggg acagggtgcg atgattaatc gaactatcgg
    11281 tcatggtgaa catgcccaac cagcaccact tccaaaggct cattttcgaa cgactaatga
    11341 gatgttggat gaatttgcct ttttgggaga ggaactggct cgtaaactgg ttattgaaaa
    11401 caccaatgcc ttggcagaaa tatttgaatc cgttgaagtc gttaagggtg acttgtatac
    11461 gcctttcatc gacaaggctg aagaaacagt tgctgagttg acctataaga aagcttttga
    11521 gatttatgga aatccgctgc cagatattgt tgatttgcgg attgaaaaag aattaacatc
    11581 catactgggg aatggatttg ctgtgattta tctggcatcg cagatgctgg tgcaacgttc
    11641 taatgaacgg ggttatttgg ttggttctcg tgggtctgtc ggatctagtt tcgttgcgac
    11701 catgattggg attacggagg tcaatcctct ctctcctcac tatgtctgtg gtcagtgtca
    11761 gtacagtgag tttatcacag atggttcgta cggttcagga tttgatatgc cccataagga
    11821 ctgtccaaac tgtggtcaca aactcagtaa aaacggacag gatattccgt ttgagacctt
    11881 ccttggtttt gatggggata aggttcctga tattgacttg aacttctcgg gagaagatca
    11941 gcctagcgcc cacttggatg tgcgtgatat ctttggtgaa gaatatgcct tccgtgcggg
    12001 aacggttggt acggtagctg ccaagactgc ctatggattt gtcaagggtt acgagcgaga
    12061 ttatggcaag ttttatcgtg atgcagaagt agaacgcctc gctcaaggag cggcgggtgt
    12121 caagcggaca acaggccaac acccgggggg aatcgttgtt attccgaact acatggatgt
    12181 ctacgatttt acgcctgttc agtatccagc agatgatgtc acggctgaat ggcagaccac
    12241 tcactttaac ttccacgata tcgatgagaa cgtcctcaaa ctcgatgtac tgggacatga
    12301 tgatccgacc atgattcgaa aacttcagga tttgtctggt attgacccta ataaaattcc
    12361 tatggatgac gaaggcgtga tggcactctt ttctgggact gatgtactag gggtaacacc
    12421 tgaacaaatt ggaacgccta cgggtatgtt ggggattcca gagtttggaa caaatttcgt
    12481 acgtggaatg gtagacgaaa cccatccgac aacctttgcg gaattgcttc agctgtctgg
    12541 tctgtcccac ggtactgacg tttggttggg gaatgctcag gatctgatta agcaaggaat
    12601 agcggaccta tcgactgtta tcggttgtcg ggacgacatc atggtttacc tcatgcatgc
    12661 gggtctggaa cctaagatgg cctttaccat tatggaacgg gtacgtaagg gtttgtggct
    12721 aaagatttca gaagaggaga gaaatggcta tatcgaagcc atgaaggcta ataaggtgcc
    12781 agagtggtat atcgaatcct gtgggaaaat taagtacatg ttccctaagg cccatgcggc
    12841 agcctacgtt atgatggcct tgcgtgtagc ttacttcaag gttcaccatc ctatttatta
    12901 ctactgtgct tacttctcca ttcgtgctaa ggcttttgat atcaagacca tgggtgcggg
    12961 cttggaggtc atcaagcgca gaatggaaga aatctctgaa aaacggaaga acaatgaagc
    13021 ctctaatgtg gaaatcgatc tctatacaac tcttgagatt gtcaatgaga tgtgggaacg
    13081 aggtttcaag tttggtaaat tagatctcta ccgtagtcag gcgacagagt tcctcatcga
    13141 cggggatacc cttatcccac catttgtagc aatggatggt ctgggagaga acgttgccaa
    13201 gcaactggtg cgggcgcgtg aagagggaga attcctctct aaaacagaac tacgcaagcg
    13261 tggtggactc tcatcaacct tggttgaaaa gatggatgag atgggtattc ttggaaatat
    13321 gccagaggat aaccagttga gtttgtttga tgagttgttt taaaaaattg cttaataatc
    13381 tattaaaaga ggctaacgta tatccaatag atttacatta gctttctttt ttgttaaaat
    13441 agtctatgga aagagggtga gagtatgtca aagatgagta taagcatccg tctggatagt
    13501 gaggttaagg agcaggccca acaggtgttt agtaatctgg gaatggatat gacaacagct
    13561 attaatattt tccttcgtca ggcaattcaa tatcagggat taccttttga tgttagacta
    13621 gacgaaaatc ggaagttgct ccaagcgtta acggatttag accaaaatcg taatatgagc
    13681 cagtcttttg aatcagtctc agatttgatg gaggacttac gtgcttaaga ttcgttatca
    13741 taaacagttt aaaaaagatt ttaagttggc tatgaagcgt ggtttgaagg cagaattatt
    13801 agaagaagtt ttgaattttc tggttcaaga aaaagaacat cctgccagat atcgtgatca
    13861 ttcattgacg gcatccaagc attttcaagg agttcgtgaa tgccataccc agccagattg
    13921 gcttttggtt tataaagtag acaagtcgga attgatttta aatttgctga ggacaggcag
    13981 tcacagtgat ttattttaat ctattttaag ggggttctca tgaaactaag aatatttgcg
    14041 gaagataagc cggctaagaa ggtatttgaa tatcaattag aacttgctga tcgtacaatt
    14101 cttctatcga cagcactctt gtcaggtgct attgctttag caggaatctt ttctgctttg
    14161 aaagaaaaat aaaaatagaa aagagaaaac agaatggttt taccaaattt taaagaaaat
    14221 ctaggaaaat atgcgaaatt gttggttgcg aacggaatta acgtgcaacc tggtcacact
    14281 ttggctctct ctattgatgt ggagcaacgt gaattggcac atctaatcgt gaaagaagct
    14341 tatgccttgg gtgcgcatga ggtcatcgtt cagtggacag atgatgtgat taaccgtgag
    14401 aaattcctcc atgccccgat ggagcgtttg gacaatgtgc cagaatacaa gattgctgag
    14461 atgaactatc ttttggagaa caaggctagc cgtcttgggg ttcgttcatc tgatccaggt
    14521 gccttgaacg gagtggacgc tgacaagctt tcagcttctg ctaaagctat gggacttgcc
    14581 atgaagccta tgcgtatcgc aactcaatct aacaaggtta gctggactgt agcagccgct
    14641 gctggacttg agtgggctaa gaaagtcttc ccaaatgctg cgagcgacga agaagcagtt
    14701 gatttccttt gggatcaaat cttcaaaact tgtcgtgtct acgaagcaga tcctgttaag
    14761 gcttgggagg aacatgcagc cattctcaag agcaaggccg atatgcttaa taaggagcaa
    14821 ttttcagccc ttcactacac agcgccagga acagatttaa cacttggttt gccaaagaac
    14881 cacgtttggg aatcagctgg tgctgtcaat gcacagggcg aagaattctt gccaaatatg
    14941 ccgacagaag aggtcttcac agcgcctgac ttccgtcgtg cagatggtta tgtcacttct
    15001 acaaaaccgc ttagctacaa cggaaatatc attgaaggca ttaaggtgac ctttaaggat
    15061 ggacaaatcg tagatatcac tgctgagaag ggtgatcagg ttatgaaaga ccttgtcttt
    15121 aaaaatgcgg gtgcgcgtgc cttgggtgaa tgtgccttgg taccagatcc aagtccaatt
    15181 tctcagtcag gcattacctt ttttaacacc cttttcgatg aaaatgcgtc aaaccacttg
    15241 gctatcggtg cagcctatgc gactagcgtt gttgatggag cggagatgag cgaagaggag
    15301 cttgaagctg cagggcttaa ccgttcagat gttcacgtag actttatgat tggttctaac
    15361 caaatggata tcgatggtat tcgtgaggat ggaacgcggg tacctctttt ccgtaatggg
    15421 aattgggcaa attaaggaga taatatgtta ggaagtatgt tcgttggtct cctagtggga
    15481 tttttagcag gtgctatgac caatcgtgga gagcgaatgg gatgttttgg aaaaatgttt
    15541 ctcggttgga tcggagcctt tctaggtcac ttgctctttg gaacttgggg gccagtttta
    15601 tcaggaacag ctattatccc agcgatttta ggagccatga ttgttttagc tattttttgg
    15661 agacgaggaa gttaatttcc taaatctgat actcaatgaa aatcaaagag caaactagga
    15721 aactagccgc aggttgctca aagcactgct ttgaggttgc agatagaact gacgaagtca
    15781 gtaacatata tacggcaagg cgacgttgac gcggtttgaa gagatttttg aagagtatga
    15841 aacgaaaagg aggttggtca ttgtactagc ctccttttga tatgatataa tagttctatg
    15901 agattagata aatttttagt tgcctgtgcg gtgggaagtc ggactgaggt caaaaacttg
    15961 ctcaaggctg ggcgcgtgac tgtaaatggt aaaaaagaaa aatcagctaa attgcagatt
    16021 gatgaaaaaa tagatgagat tcgctttgat gggcaagtgt tggagtatga agagtttgtc
    16081 tactacatga tgaacaagcc caaaggagtt atctcagcga ctgaggatcc caagcacaga
    16141 accgttctgg acttgttgga tgacttggcg cggagtaagg aagtttttcc agtaggacgc
    16201 ttggatattg acacgcatgg tcttttgctc ttgaccaata atggtcagct ggctcatgtt
    16261 cttctttcgc ccaagcgtca tgtggacaag acttatcagg ctcaggtcaa gggaattatg
    16321 acccaagaag atgtggagac atttatcaag ggcattccgc tcaaagactt tacctgtcag
    16381 cccgctagac tggagattct ttccacagat gcagaaaaga atcagagcca aatccgtgtg
    16441 accattgcag aagggaagtt tcatcagatc aagcgtatgg tgggctactg tggcaaggaa
    16501 gtggtggact tgcaacgatt gactatggga actttagtat tagatgagaa cctggaacga
    16561 ggggaatggc gtcgcttgac caaggaagaa ttagaaattc tccgcgctaa tattatttaa
    16621 tttagtttaa tactcaatga aaatcaaaga gcaaactagg aaactagccg caggctgtac
    16681 ttgagtacgg caaggcgacg ttgacgtggt ttgaagagat tttcgaagag tataaattcg
    16741 aaaaaggtga gggacaatgt cctcgccttt tatgtttttt agttgcttcc tttgtgaaaa
    16801 gagttataat agactgtaga ataaaaggag gaatctatga acgcgattca agaatcattt
    16861 actgataaac tatttgccaa ctatgaagca aatgtaaaat accaagcgat tgaaaatgct
    16921 gccagccaca acggaatttt tgcagctctg gaatgtcgcc aaagccatgt agacaacaca
    16981 cctgttttct cattggattt aaccaaggac aaggtcacta accagaaagc gtctggtcgt
    17041 tgctggatgt ttgcggctct caacaccttc cgccacaaac tcatctcgca atacaaattg
    17101 gagaactttg agttgtcaca agcccacact ttcttctggg ataagtatga gaaatcaaac
    17161 tggttcttgg agcaagtcat tgcgacttca gaccaagaat tgactagccg caaggttagc
    17221 ttcttactcc aaacacctca acaagatggc ggtcaatggg atatggtcgt ttccctcttt
    17281 gaaaaatacg gtgtcgtgcc taagtcagtt tatcctgagt ctgtttcatc tagcagcagt
    17341 cgtgagctaa atgcgatcct taataaattg cttcgtcaag atgctcaaat cttgcgtgat
    17401 ttgcttgttt ctggtgcaga tcaagcgact gttcaagcta agaaagaaga cctcttgcaa
    17461 gaaatcttta actttcttgc tatgtcatta ggacttccac cacgcaagtt tgactttgct
    17521 tatcgcgata aagataacaa ctataaaagt gaaaaaggaa tcacaccaca agagttttac
    17581 aagaaatatg tcaatcttcc tttagaagac tacgtttctg ttatcaatgc tccaactgct
    17641 gataaacctt acggaaaatc ttacacagtt gagatgttgg ggaatgtggt tggtagccgt
    17701 gcagttcgct acatcaacgt tccaatggag cgcttgaaag aattggcgat tgcccaaatg
    17761 caagcaggtg agactgtttg gtttggttct gatgtcggcc agctcagcaa ccgtaaggct
    17821 ggcatccttg cgacagatgt ttatgacttt gaatcaagca tggacattaa acttactcaa
    17881 gacaaggctg gacgtttgga ctatagcgaa agcttgatga cccacgccat ggtcttgaca
    17941 ggtgttgact tggacgaaaa tggtaaatca accaagtgga aggttgaaaa ctcatgggga
    18001 gacaaggtcg gtacagatgg ttactttgtt gcctcagacg cttggatgga cgaatacaca
    18061 taccaaatcg ttgttcgtaa ggaattgctg acagcagaag aacaagctgc ctatggagca
    18121 gaaccaatcg tacttgcacc atgggatcca atgggagcct tggctgaata aaagcataga
    18181 aaaaaggaat cagattttag aacctggttc ctttttagtt gcttgattac atgatgtgaa
    18241 gaacatgtgc cacaataccc actgcgaaga gggcaaggat aatagtgatt ggagatactt
    18301 ttttcttaag taagtacatg caaagtaaag taaggagtag tcctgataat ccaggaatca
    18361 acatatccaa gttttgttgg aaagtagtaa ctttttcagg agtttgagac aatccttgtc
    18421 ctacttgtgc gaatgcttct tggatacctt tagacccttc tggcaattta tcccaatgga
    18481 tataagcctt ttcatctagt tgaactttag aaacatcgaa agcaaattta atatttaccc
    18541 agcgttgaac aaggacagca agaatgaaca tcccaaggat agaagctcct ttagtgatat
    18601 cttgaaggat accaccagac atatctttag tgatttctga tccagccttg tatccaatct
    18661 cttgaacata ccacaagaat gacatacgaa tcaagttcca tgcaacaaag aagaggagtg
    18721 gccccaagat attgccagta agggcaagtg aagcaccgag agatccaagg attgggcgta
    18781 ctgtaaacca gaatactggg tcaccgatac cagcaagagg tcccatcata ccgattttaa
    18841 ccccttggat agcagcgtca tcgatttcca caccgttagc acgttcttct tcaagcgcaa
    18901 gagtaacccc catgactgga gcagctacgt atggatgagt gttgaagaac tcaaggtgac
    18961 gctcaagagc agcgatttga tcttcttttt tagtatagag ttttttgata gctggaatga
    19021 gtgtataagc ccagcccaag ttttgcatcc gttcaaagtt ccaagaccct tgtaagaagg
    19081 ttgaacgcca ccaaactttt ttacgatctg atttagttaa ttgaagtttt tcagtcatga
    19141 tgttttcagt cctttcttat cttagtagtc ttctaggata tcgccgattg ggtcgttaga
    19201 agttgcggct cctccgccac catttccacc agttttagaa aggtgaaggt agataagagc
    19261 gatagcaaca ccgatagcac cgaatccgat tagagtaata tctgacacag cagcaagaac
    19321 gaaaccaaga gcgaagaatg gccatacttc acgagttgcc atcatgttga taaccatggc
    19381 gtaaccaacg gcaacgacca taccaccacc gatagccata ccatctttga gccagtctgg
    19441 catggcacta aggatacttt gtacagtttc agttggtacc ataaggagaa gagctgcagg
    19501 aagcgcgata cgaagtcctt ggaaaagtag cgcaatgaaa tgagcacgct ccacagcgcc
    19561 gaagtcacct tttttagcgg cagcatcagc agtgtgaacc aatccaactg aaattgtacg
    19621 aacaatcata gttaagaata gaccagctac ggcaagaggg atagcaaccg cttgggcaac
    19681 accgatacca gtcttggtaa agtcaccacc aagaaccatg ataatggcag cagcgacaga
    19741 agcaagtgca gcatcaggag cgatagcagc accgatattt gaccaaccaa gggcaatcat
    19801 ttgaagcgat ccaccgagga taatccctgc ttccaagtga cctgttacaa gcccaataag
    19861 ggtacaggct acaagtggtt ggtgaaattg gaactggtcg aggatgcctt caagacctgc
    19921 aaagaaggct acaacgacta ctaaaaccat agaaataata gacatgttta aaatcctttc
    19981 ataaataatg gcttatttga cattggcttt gttaatcaag tcaaacaaat cttttttaga
    20041 atcatttggt actttacgga catcaaattc aacacccaag tcacgcattt tttcaaatgt
    20101 agcaacatct tctttgtcca tagacaaaac ggtattgacc aatgttttac ctgttgagtg
    20161 agccatagaa ccaacgttaa gagtcttgat tggcacgccg ccttcgatgg cacgaagggc
    20221 atcttgaggt gtttcaaaca agataagggc atgtgtttct ccaaaacgtg ggtcttttga
    20281 aatctcaatc agtttttgaa ttggaaccac gttagccttg acattacctg gagctgcttg
    20341 tttaatcaat tctttacgaa ggtcgtcttt agccacgtta tctgaagcaa cgatgatacg
    20401 atttgctttt gaatctggag tccaagcagt tgcaacctga ccgtgaagta gacgtgtgtc
    20461 aagacgggca agattgattt tcaatttacc gtctccgata acagttcctt ctgggatagc
    20521 agtttgggca actggagcag ctgcagcgct tgcaacttct tcaactggat ttagctcttc
    20581 tggaagagct ttgatgccat ctttggcttc tttaatgata ttagcagcga ctttttctac
    20641 acctgcagca gcgtccatga ggcgctctgt gtaggcttga atcaacatcg gtaagttaag
    20701 tcctgtgatg atggcaaact tacgctcagg attttctccc atcacgcgac tagcttggtt
    20761 aaatggagaa ccactccaaa ggtcagccaa aactagaacc tcatcttctg cgtcaaatgc
    20821 agcaacagcg ttattaaact tagcgtatag atcatcagga ccttcatttg gcataaaggt
    20881 cacaacttga accttttctt gttcaccaaa gatcatagat cctgactgat gaatacccgc
    20941 agcaaattcg ccgtggctcg caataatgat tccgatactc attattgtca ttcctccttt
    21001 tttgttttag ttttcattta agaaaacttt aagactgttt aagtataaac cgttttcata
    21061 aaaaaagcaa ctatttatca taaaaaatta aaaaattttt atttttgtat gttgatatgt
    21121 tgagtttttg aggagtaatc ttgaattctt atttaaaatt taatataaaa aagttggaag
    21181 aagcttccaa cttttaaaat aggtttctat taaattagtg ggtgaagtcg agtaccatac
    21241 gtccttggat ttggcctttt tccatttcgt cgaaaatggc aacagcatct tctactggac
    21301 gtttttgaac aactgggact accagacctt ctgcaccaaa ttggaaggct tcttctaagt
    21361 ctttacgagt tccgacaaga gaaccgatga cttggattcc atcgaggact gttttaacga
    21421 tgctgagttc catcatttca gaaggaagac caacagcgac gacgcgacca ccagcacgaa
    21481 tggagtcaac agcctggttg aaggcaactt tagacacagc agttacgaca gctgaatgag
    21541 ctcctccatc agttttttct ttaatgagtc cagctacatc ttcaacttcg aggccgttaa
    21601 tcacaatgtc agcgcctact tcttttgcaa gggtaagttt gtcattgttg atatcgactg
    21661 cgataacatg agcattgaat acttttttag cgtattgaac agcgaggtta ccaagtccac
    21721 cagcaccgta aagaacaacc cattggcctg gttcaacttt tgcttctttg atagctttat
    21781 aggttgttac tccagcacat gtgatagaag aagcttgggc tggatcaagt ccgtcaggaa
    21841 ctttgacagc atagtcagca gttacgatac attgttcagc cataccaccg tctactgagt
    21901 agccagcatt tttcactgta cggcaaaggg tttcgcgacc agttgtacag tattcgcaag
    21961 tgccacatcc ttcaaagaac caagcaacgc tgacgcggtc accgacttta aggcttttca
    22021 catctggagc aatctcttta acgataccga taccttcgtg cccaagaaca cgtcctggga
    22081 cttgaccaaa gtcaccatga gcaacgtgga ggtcggtgtg gcaaacgcca cagtattcaa
    22141 cttctacaag tgcttcccca gtttcaagtg gacggagtac tttttcttca atagcaacac
    22201 cagtgctttc tggatttaca acaacagctt tcatagctat cctccttgat attgaccgag
    22261 aacagggaca acgggactgg attgattttt tgtcaacaga gtcgagtgtg acgagctctc
    22321 ttgtatttat atgtgaagta tatcacaaaa ggaagccttt tccaaggttt tggagacaaa
    22381 aagtagaaca atcgattaac tggttgatga gactgtgaaa agagcacaaa gaccagcttg
    22441 caagctggtc atactcttcg aaaatctctt caaaccacgt cagcttcatc tacaacctca
    22501 aagcagtgct ttgagcaacc tacggctagc ttcctagttt gctctttgat tctcattgag
    22561 tatcagtata ggttataaga ataagtcttg tagggttttg gcaaccccat cttggtcgtt
    22621 ggtaagggaa atttgctcat ctgcataagg gagtagctct ggattggcat ttttcatggc
    22681 ataacccttc ccagcaaaag cgagcatttc ggtatcattg tgttcatctc caaaggcaat
    22741 caaatctttt ttgtcacgat tcattatctt gagcaagtag tccaaagcaa aggccttgtt
    22801 gacacctttt ggggtacatt caaggatatt gagcggacct ccccaggtat tgatagaaag
    22861 ttgatgctgg tagaaggcgt tcatttcttt tgccaaggca tatttgtcac tggctctggt
    22921 ctgcaagagg atacagttag ggtccttggt caccaattca ggctggaatt gatcttcagg
    22981 ctggaaagct tctacaccaa atagtttggg attggcaatt tcttcattgg gatttgtaat
    23041 gtagaatttt ttacgatatt ctccagcgat aaaatcggct tgaatgtcct ctgaacgttg
    23101 aaccatatct agcagatatt ttttgtctac agtcaaacac ttttcaaaat cccaaacttg
    23161 gtctggtaaa tgagtaaggg atccgttgaa gttaatcata ggagtgtcta agcccagttc
    23221 acggtaaaaa tcttttgaca tacggtaagg gcgacctgtc gtaataataa cctgatggcc
    23281 tttttcagca acttttttga tggttctttt ggtaaagtca gaaatttgac tgtctgagtt
    23341 gagcagggtt ccatccaggt caactgcaat aatttttttc gtcatatgga cctttctagt
    23401 gtgttttcag ataagatgtc ttctattata acagaaagca ggcagaaaag cagattccaa
    23461 acaaaaaatg aaattggatt aatttcttaa acaaatcaaa caaagacatt gaaaaagtga
    23521 atgataaatg atataattct attattgttc gtaaaaatta aaaggagatt gatgatggac
    23581 aaattattta aactaaaaga gaacggtaca gacgttcgta cagaggttct cgctggttta
    23641 acaactttct ttgcaatgag ctatattctc tttgtaaacc cacaaatact ttcacaaaca
    23701 ggaatgcctg ctcagggcgt cttcctagcg acgattattg gtgcagtagc gggtaccttg
    23761 atgatggctt tttatgctaa cttaccttat gcccaagcac caggtatggg actcaatgcc
    23821 ttctttacct ttacagttgt attcgggctt ggttattctt ggcaagaagc cctagctatg
    23881 gtcttcatct gtgggattat ttcattgatt attaccttga caaatgttcg taaaatgatc
    23941 attgaatcga ttcccaatgc tcttcgctca gctatttcag ctggtatcgg tgtcttcctt
    24001 gcctatgtag ggattaagaa tgctggactt ttgaaattca cgattgatcc aggcaactat
    24061 actgttgtag gagaaggggc tgacaaagct caagcaacga ttgcagcaaa ctcttcagca
    24121 gttccaggat tggtcagctt taataatcca gctgttttag tggctcttgc aggacttgcc
    24181 attactatct tctttgtcat caaagggatt aaagggggaa ttattctctc tatcttgaca
    24241 acaactgttc ttgctattgc agttggtttg gttgatttgt ctagtatcga ttttgctaat
    24301 aaccatgttg gtgcagcttt tgaagatttg aagacaatct ttggtgcagc tcttggttca
    24361 gaaggtttgg gagctttggt ttcagataca gctcgcttgc ctgaaactct gatggccatt
    24421 cttgccttct cattgacaga tatttttgac acaattggta ccttgatcgg tacaggtgaa
    24481 aaagttggta ttgtagcgac aaatggtgaa aatcaccaat cagccaaatt ggataaggct
    24541 ctttactctg atttgattgg aacgacagtc ggtgccattg caggtacttc aaacgtaacg
    24601 acttatgttg agtctgctgc tggtatcggt gcaggtggac gtactggttt gacagccttg
    24661 gttgtagcta tctgttttgc gatttcaagc ttctttagcc cacttctagc gatcgtacca
    24721 acagcggcta cagctccaat cttgattatc gttgggatta tgatgcttgg tagcttgaaa
    24781 aatatccatt gggatgatat gtctgaagca gttcctgcct tcttcacatc tatctttatg
    24841 ggattcagct actctatcac tcaagggatt gcagttggtt tcttgactta cactttgact
    24901 aagcttgtca aaggtcaagt taaagatgtt catgccatga tttggatttt ggatgccttg
    24961 tttatcctta actacatcag catggcctta taatagaatg acccaggggg atttcccccc
    25021 ttttttaata caaaggagat aggtgatgaa agagaaaaat atgtggaaag aattgttgaa
    25081 tcgtgcaggc tggattttgg tctttttact tgccgtcctt ttatatcagg ttcccctagt
    25141 ggttacctct attttgactt taaaagaagt agccctgcta cagtcagggc tgatagttac
    25201 tggcctttca attgtggttc tggctctatt tattatggga gctcgtaaaa ccaagttagc
    25261 tagttttaat ttttcttttt ttagagctaa agatttggca cgtttgggct tgagttattt
    25321 agttattgtc gggtcaaata tacttggttc tatcttgttg caactgtcaa atgagacgac
    25381 aacagctaac cagtctcaga ttaacgatat ggttcagaat agttctctga tttctagctt
    25441 cttcttgcta gccttgcttg ctccaatttg tgaggaaatc ttgtgccgtg ggattgttcc
    25501 taaaaagatt ttccgaggca aggagaactt gggatttgta gtcggtacga ttgtgtttgc
    25561 tttattgcat caaccaagta atttaccttc tttattgatt tatggaggta tgtcgacagt
    25621 tctatcttgg acggcctaca agacccaacg tttggaaatg tcgatcttgc ttcacatgat
    25681 tgttaatggg attgctttct gtttgttggc tcttgtggtg attatgagtc ggacattagg
    25741 aatttctgtg taaaagtttt tatgtaggaa ccgacctctt tctactaggg aaagatgaat
    25801 gcaatcgtgt ccatcttttt ctttttatgg taaaatagaa aaataatatg atgaaaatcc
    25861 ttgagggagt gaccgatatg tcaagtaaag ccaatcatgc aaagacagtt atttgcggaa
    25921 ttatcaatgt aaccccagac tccttttcgg acggtggtca attttttgct cttgagcagg
    25981 cactccagca ggctcgtaaa ttgatagcag aaggagccag tatgctcgat atcggcggag
    26041 aatcgactcg gccgggcagt agctatgttg agatagaaga ggaaatccag cgtgttgttc
    26101 cagtgatcaa agcgattcgc aaggaaagtg atgtccttat ttctatcgat acttggaaaa
    26161 gtcaggtggc agaggctgct ttggctgctg gtgccaatct agttaatgat atcactggtc
    26221 tcatgggcga tgaaaaaatg gcccatgtgg ttgctaaagc tggagcgaaa gtagtcatca
    26281 tgtttaatcc agtcatggct cgacctcaac atcctagttc gcttatcttc cctcattttg
    26341 gctttggtca aacctttaca gaaaaagagt tagctgactt tgaaacattg ccaatcgaag
    26401 acttgatggt ggctttcttt gaacgagcac tagcgagagc ggcagaagct ggtattacac
    26461 cagaaaatat cctgttggat ccaggaattg gctttggtct gaccaagaaa gaaaatctcc
    26521 tccttttacg ggacctggat aaactacatc agaagggcta tccaatcttt ctcggagtgt
    26581 cgcgcaagcg atttgtcatc aatatccttg aagaaagtgg gtttgaagtc aatcctgaga
    26641 cagagcttgg tttccgaaat cgggacacgg cttcggctca tgtaactagt atcgctgcga
    26701 gacagggtgt agaagtggtg cgcgtgcatg acgtagctag tcacaggatg gcagttgaaa
    26761 ttgcctctgc cattcgtctg gctgatgaag cggaaaattt agatttaaaa caatataaat
    26821 aagatgaaag aaattgaaaa caatcagtgg attgctaact accggacgga tcaaccgcat
    26881 tttggattgg aacgaatggt ggaactgtta gctttgcgtg gcaatcccca tctcaaactc
    26941 aaggtcctcc atatcggagg gactaacggc aagggctcga ctattgcttt tttgaaaaag
    27001 atgctagaaa agctagggct gagagttggc gtgtttagct cgccctatct cattcattac
    27061 acagaccaga ttagcatcaa tggggaatcg atctcagaag cgaggctaga agctctcatg
    27121 gcagactatc agtctttgct ggagggagaa gcggtcgcca atttacaggg cacaaccgag
    27181 tttgagatta tcacagccct ggcctatgac tactttgcct cagagcaagt agatgtggcc
    27241 atcatggaag ttggcatggg tggacttttg gatagtacca atgtctgtca gcccattttg
    27301 acaggaatta caactattgg cttggatcat gtggccctac ttggtgacac cttggaggcc
    27361 atagcagagc agaaggcagg tattatcaaa caagggatac ccttggtaac agggcgtatt
    27421 gctccagaag ccttgactgt gattgaccgc attgcggaag ggaaagatgc gccgagactt
    27481 gcctacggga cagattatca ggttcgtcat caagaaagtg tggtgacagg ggaagtcttt
    27541 gactatacaa gtgctgtcag acaaggtcgc ttccagacta gcctgcttgg tttgtaccaa
    27601 atagagaatg ctgggatggc catagcttta cttgatactt tttgtcaaga agatggtcga
    27661 gagctagcaa gcaatgactt tcttggtcaa gccttggaag aaacaagttg gccagggcgt
    27721 ttggaaatcg tgtcaagaga tcccttgatg attttggatg gagcccataa tccccatgct
    27781 atcaaggcct tgttggtaac cttgcaagaa cgttttgcgg attatcataa ggaaatcctc
    27841 ttcacttgta tcaaaaccaa agccttggag gatatgttgg acttgctggg agccatgcca
    27901 gataccgagc ttactctaac acattttgcg gatagtcggg cgacggatga aagcgtgctg
    27961 aaagaggcag ctaagtctag aaatctcagc taccaagatt ggcatgattt tctagagcag
    28021 aatttgacag ataaaaaaga agagaaacaa acagttagga ttgtcacagg ttccttgtat
    28081 ttcttgagcc aagtgagggc ctatctgatg gagaggaaga acgagaatgg atacacaaaa
    28141 gattgaagca gctgtaaaaa tgattatcga ggctgtagga gagaacgcta atcgcgaggg
    28201 cttgcaggaa acacctgctc gtgtagcccg tatgtatcaa gagatttttt caggtcttgg
    28261 tcaaacagca gaggaacatt tgtcaaaatc ctttgaaatt attgacgata atatggtggt
    28321 agaaaaggat atctttttcc ataccatgtg tgaacaccac ttcttgccat tttatggtag
    28381 agcgcacatt gcctacattc cagatggtcg tgtggcaggc ttgtctaagc tagcccgtac
    28441 ggttgaagtt tattcgaaaa aaccacaaat tcaagaacgt ttgaatatcg aagtggccga
    28501 tgccttgatg gactatctag gtgctaaagg agcctttgtt gtcattgagg cggaacatat
    28561 gtgtatgagt atgcgtggtg ttagaaaacc aggcactgca accttgacga cagtagctcg
    28621 tggtctattt gaaacagata aggacctccg cgaccaagct tatcgtttaa tggggctata
    28681 aaaagaatcc gcttcaagcg gatttttcta gaaaggaatc attatggatc aactgcagat
    28741 taaggatttg gaaatgtttg cctatcatgg tctttttcct agtgagaaag aattggggca
    28801 gaaatttgtc gtttcagcca tcctatccta tgatatgacc aaggcagcta cagacttgga
    28861 tttaacagcc tctgtccatt acggagaatt atgtcagcag tggacgactt ggtttcagga
    28921 aacgagtgaa gatttgattg aaacggtagc ctataaactg gtggaacgta cctttgagtt
    28981 ttatcctctt gtccaagaaa tgaagttgga actgaaaaaa ccttgggcac cggtgcattt
    29041 gtcactagat acttgctcgg taaccattca tcgccgcaag caacgagcct ttatcgccct
    29101 aggaagcaat atgggagata aacaagcaaa cttgaagcaa gccattgaca aactgcgagc
    29161 tcgtggcatc tatattctca aagagtccag tgtcttagcg acggagcctt ggggtggagt
    29221 ggagcaggat agctttgcca atcaagtggt tgaggtggaa acctggctac cagcacaaga
    29281 cttgttagaa accttgttag ccattgagtc agagctggga cgggtgagag aagtgcattg
    29341 gggacctcgt ttgattgatt tggacttgct ctttgtggag gaccagatcc tttatacaga
    29401 cgacctcata ttgcctcatc cttacatagc ggaacgcctt tttgtccttg agtccttaca
    29461 ggaaattgcg cctcatttta tccatccgac attaaaacaa ccgatccgca acttgtatga
    29521 tgctttgaaa aaatagaaaa actctagttt tcagttactt gcaactgaag gctagagttt
    29581 ttaaaatagg tcattttctt ctgggaggag gatagtttct ctaccgtaca tgtctaaaac
    29641 cagtactctt gggggataac gagggtcgaa aggatggtta aagtcaaaat caatggctgt
    29701 agggaggtgt tgacttgaaa agtggaaggt aatctttcct tggttattaa gcaattgaaa
    29761 ctcgagttct tcttccaatt caaagacatt ttttaagaaa tgatcgatga tataccaaaa
    29821 agagtcaatg atgtcatcgg gcaagctggt aacaatgcca aaactagcag accgcatgtg
    29881 ggtattggta aaagccatat ctctgtcccc tttcttttcc cttatcatac agcaaatagg
    29941 attaaaaatc aagaaaaggt gattttttga aaaggatttt aactacaggg aaaaataagg
    30001 aaaaaaattc ccaaaaatct accgaagtta ataggtaaat tcccaaatta acttgattat
    30061 ataactttca gtta
//
LOCUS       DAVBCV010000023        28613 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBCV010000023 DAVBCV010000000
VERSION     DAVBCV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.023"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..236)
                     /locus_tag="VQ516_000996"
     CDS             complement(<1..236)
                     /locus_tag="VQ516_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HET2511455.1"
                     /translation="MYEESKQLTNAQFKRLVGVQRTTFKEMLAVLKTAYQLKHAKGGR
                     KPKLSLEDLLMGTLQYVREYRTYEEIAADFGIHE"
     gene            582..1535
                     /locus_tag="VQ516_000997"
     CDS             582..1535
                     /locus_tag="VQ516_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2511456.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLISLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1586..2776
                     /locus_tag="VQ516_000998"
     CDS             1586..2776
                     /locus_tag="VQ516_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2511457.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            2925..3296
                     /gene="rnpA"
                     /locus_tag="VQ516_000999"
     CDS             2925..3296
                     /gene="rnpA"
                     /locus_tag="VQ516_000999"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2511458.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            3271..4095
                     /locus_tag="VQ516_001000"
     CDS             3271..4095
                     /locus_tag="VQ516_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2511459.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            4114..5100
                     /locus_tag="VQ516_001001"
     CDS             4114..5100
                     /locus_tag="VQ516_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2511460.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            5151..5774
                     /locus_tag="VQ516_001002"
     CDS             5151..5774
                     /locus_tag="VQ516_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET2511461.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPGWQLQQTSQWMNQAQRLRPHFWVYLQRDEQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            5905..7362
                     /locus_tag="VQ516_001003"
     CDS             5905..7362
                     /locus_tag="VQ516_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2511462.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7385..7666
                     /locus_tag="VQ516_001004"
     CDS             7385..7666
                     /locus_tag="VQ516_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2511463.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7743..8228
                     /locus_tag="VQ516_001005"
     CDS             7743..8228
                     /locus_tag="VQ516_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2511464.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8245..8910
                     /locus_tag="VQ516_001006"
     CDS             8245..8910
                     /locus_tag="VQ516_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2511465.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            8914..9777
                     /locus_tag="VQ516_001007"
     CDS             8914..9777
                     /locus_tag="VQ516_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2511466.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9779..10483
                     /locus_tag="VQ516_001008"
     CDS             9779..10483
                     /locus_tag="VQ516_001008"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2511467.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10662..12335
                     /locus_tag="VQ516_001009"
     CDS             10662..12335
                     /locus_tag="VQ516_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2511468.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12447..13538
                     /gene="ulaG"
                     /locus_tag="VQ516_001010"
     CDS             12447..13538
                     /gene="ulaG"
                     /locus_tag="VQ516_001010"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2511469.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13652..15628
                     /gene="tkt"
                     /locus_tag="VQ516_001011"
     CDS             13652..15628
                     /gene="tkt"
                     /locus_tag="VQ516_001011"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2511470.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVTDYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15745..16044
                     /gene="yajC"
                     /locus_tag="VQ516_001012"
     CDS             15745..16044
                     /gene="yajC"
                     /locus_tag="VQ516_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2511471.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVEGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            16091..16519
                     /locus_tag="VQ516_001013"
     CDS             16091..16519
                     /locus_tag="VQ516_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2511472.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16521..16931
                     /locus_tag="VQ516_001014"
     CDS             16521..16931
                     /locus_tag="VQ516_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511473.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17231..19882
                     /gene="adhE"
                     /locus_tag="VQ516_001015"
                     /gene_synonym="adhC"
     CDS             17231..19882
                     /gene="adhE"
                     /locus_tag="VQ516_001015"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2511474.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(20867..21175)
                     /locus_tag="VQ516_001016"
     CDS             complement(20867..21175)
                     /locus_tag="VQ516_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2511475.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(21172..21480)
                     /locus_tag="VQ516_001017"
     CDS             complement(21172..21480)
                     /locus_tag="VQ516_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2511476.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(21485..22780)
                     /locus_tag="VQ516_001018"
     CDS             complement(21485..22780)
                     /locus_tag="VQ516_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2511477.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPSAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGIVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(22798..24207)
                     /locus_tag="VQ516_001019"
     CDS             complement(22798..24207)
                     /locus_tag="VQ516_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2511478.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSLCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            24365..25093
                     /locus_tag="VQ516_001020"
     CDS             24365..25093
                     /locus_tag="VQ516_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2511479.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            25553..25747
                     /locus_tag="VQ516_001021"
     CDS             25553..25747
                     /locus_tag="VQ516_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2511480.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(26113..27423)
                     /locus_tag="VQ516_001022"
     CDS             complement(26113..27423)
                     /locus_tag="VQ516_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2511481.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            27660..28532
                     /locus_tag="VQ516_001023"
     CDS             27660..28532
                     /locus_tag="VQ516_001023"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2511482.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         8961 a         5098 c         5958 g         8596 t
ORIGIN      
        1 ctttcgtgga taccaaaatc agccgcaatt tcttcataag tgcggtattc tcgcacatat
       61 tgaagagtgc ccataagaag gtcttctagg cttaatttag gttttcgtcc accttttgcg
      121 tgtttaagtt gataagctgt ttttaataca gctaacatct ctttaaaagt cgtgcgctga
      181 acaccaacaa gacgcttaaa ttgtgcattg gttagttgtt tactttcttc ataaatttta
      241 ttgtatcata tttttgtttc acaggaagtc taataaataa aaattcttta aaattttgtt
      301 gggaaaatgg gaaaagatat aaatggaaac ttagtataac acttggaaaa ctctgtaaaa
      361 atagtaagtc aattcatgtc ccgttttttc tctagggaag ttgggcggct atatttatat
      421 gaccatttgt cgtaataaac atccctacca agggtcaata gaaataaatt caacgaaact
      481 tttgctgtca gatttagatg cccaatttgt catttatact ctttcatata ggactttcta
      541 taaggatgga aaaaagaagg gaaatttgat aagataggaa tatggatttt gaaaaaattg
      601 aacaagctta tacctattta ctagagaatg tccaagtcat ccaaagtgat ttggcgacca
      661 acttttatga cgccttggtg gagcaaaaca gcatctatct ggatggtgaa actgagctaa
      721 accaggtcaa agacaacaat caggccctta agcgtttagc actacgcaaa gaagaatggc
      781 tcaagaccta ccagtttctc ttgatgaagg ctgggcaaac agaacccttg caggccaatc
      841 accagtttac accggatgct attgctttgc ttttggtgtt tattgtggaa gagttgttta
      901 aagaggagga aattactatc ctcgaaatgg gttctgggat gggaattcta ggcgctactt
      961 tcttgatctc gcttgataaa aaggtggatt acttgggaat ggaagtggat gatttgctga
     1021 ttgatctggc agctagcatg gcagatgtaa ttggtttgca ggctggcttt gtccaaggag
     1081 atgccgttcg tccacaaatg ctcaaagaaa gcgatgtggt catcagtgac ttgcctgttg
     1141 gctattatcc tgatgatgcc gttgcgtcgc gccatcaagt tgcttctagc caagaacata
     1201 cttacgccca tcacttgctc atggaacaag ggcttaagta cctcaagtca gacggatacg
     1261 ctatttttct agctccgagt gatttgttga ccagtcctca aagtgatttg ttaaaagaat
     1321 ggctgaaaga agaggcgagt ctggttgcta tgattagtct gcctgaaaat ctctttgcta
     1381 atgctaaaca atctaagact atttttatcc tacagaagaa aagtgaaata gcagtggagc
     1441 cttttgttta tccacttgct agcttgcaag atgcaagtgt tttaatgaaa tttaaagaaa
     1501 attttcaaaa atggactcaa ggtactgaaa tataaaatag attttgttat aatagttgaa
     1561 aacgcttaaa aaggggtatc atgttatgac aaaaacaatt gcaatcaatg caggaagttc
     1621 aagtttgaaa tggcaattat acttaatgcc agaggaaaaa gtgttagcca aaggtttgat
     1681 tgaacgtatc ggtttgaaag attcaatttc aactgtaaaa tttgacggcc gttctgaaca
     1741 acaaattttg gatatcgaaa atcatacaca agctgttaaa attttattgg atgacttgat
     1801 tcgtttcgat attatcaagg cttatgacga gattacaggt gttggccacc gtgttgtagc
     1861 aggtggagaa tatttcaaag aatcaacagt tgttgaggga gatgttttag aaaaagttga
     1921 agagttgagt ttgttggctc ctctacacaa cccggccaat gcagcaggtg ttcgtgcttt
     1981 caaggaattg ttgccagaca ttaccagtgt agttgttttt gatacttcct tccacacaag
     2041 tatgccagag aaagcttatc gctaccctct accaacaaaa tattacacag aaaacaaggt
     2101 tcgtaaatac ggtgctcatg gtacaagtca ccagtttgta gcaggagaag ctgcaaaact
     2161 cttgggacgt ccattagaag acttgaagtt aattacctgt catattggta acggaggctc
     2221 aattacagct gtgaaagccg gcaaatctgt agacacttct atggggttca ctcctcttgg
     2281 tggtattatg atgggaacgc gtacagggga tattgatcca gctatcattc cttatttaat
     2341 gcaatataca gaggatttta acacaccaga agatatcagt cgtgttctta accgtgaatc
     2401 aggtcttttg ggagtttctg ctaattctag cgatatgcgc gatatagaag cagctgtagc
     2461 agaagggaat cacgaggcta gcttggctta tgaaatgtat gttgaccgta tccaaaaaca
     2521 tatcggtcag taccttgcag tgctaaatgg aacagatgcc attgttttca cagcaggtgt
     2581 cggtgaaaat gcagagaatt tccgtcgtga tgtaatctca gggatttcgt ggtttggttg
     2641 tgatgttgat gatgaaaaga atgtctttgg cgttacagga gacatctcaa cagaggcagc
     2701 taaaatccgt gtcttggtta ttccaacaga tgaagaatta gtcattgccc gtgacgttga
     2761 acgcttgaaa aaataagtga aactaaaaaa atattcaata caaggagttg ggaaagttat
     2821 ttttccagct tctttttctg atgaaattgt ccaaaacctt gctatgattg gcttttttga
     2881 aaaatatggt ataatagtag taatttaata gatggagttg agttttgaag aaaaactttc
     2941 gtgtaaaaag agagaaagat tttaaggcga ttttcaagga ggggacaagt tttgctaatc
     3001 gcaaatttgt ggtctaccaa ttagaaaacc agaaaaacca ttttcgagta ggtctatcag
     3061 ttagcaaaaa actggggaat gccgtcacta gaaatcaaat taagcgacgg attcggcata
     3121 ttatccagaa tgcaaaaggg agtctggtag aagatgtcga ctttgttgtc attgctcgaa
     3181 aaggagtcga aaccttggga tacgcagaga tggagaaaaa tctactccat gtattaaaat
     3241 tatcaaagat ttaccgggaa ggaaatggga gtgaaaaaga aactaaagtt gactagtttg
     3301 ctaggactgt ctctgttaat catgacagcc tgtgcgacta atggggtaac tagcgatatt
     3361 acagccgaat cggctgattt ttggagtaaa ttggtttact tctttgcgaa aatcattcgc
     3421 tttttatcgt ttgatattag tatcggagtg gggattattc tctttacggt cttgattcgt
     3481 acagtcctct tgccagtctt tcaggtgcaa atggtggctt ctaggaaaat gcaggaagct
     3541 cagccacgca ttaaggcgct tcgagaacaa tatccaggtc gagatatgga aagcagaacc
     3601 aaactagagc aggaaatgcg taaagtattt aaagaaatgg gtgtcagaca gtcagattct
     3661 ctttggccga ttttgattca gatgccggtt attttggcct tgttccaagc cctatcaaga
     3721 gttgactttt taaagacagg tcatttctta tggattaacc ttggtagtgt ggatacaacc
     3781 cttgttcttc cgattttagc agcagtattc acctttttaa gtacttggtt gtccaacaaa
     3841 gctttgtctg agcgaaatgg cgctacgact gcgatgatgt atgggattcc agtcttgatt
     3901 tttatctttg cagtttatgc gccaggtgga gtcgccctat actggacagt gtctaatgct
     3961 tatcaagtct tgcaaaccta tttcttgaat aatccattca agattatcgc agagcgcgag
     4021 gccgtagtac aggcacaaaa agatttggaa aatagaaaaa gaaaagccaa gaaaaaggct
     4081 cagaaaacga aataaataag gaggaatctg gtaatggtag tatttacagg ttcaactgtt
     4141 gaagaagcaa tccagaaagg attgaaagaa ttagatattc caagaatgaa ggctcatatc
     4201 aaagtcattt ctagggagaa aaaaggcttt cttggtctat ttggtaaaaa accagcccaa
     4261 gtggatattg aagcgattag tgaaacgact gttgtcaaag caaatcaaca ggtagtaaaa
     4321 ggcgttccga aaaaaatcaa tgatttgaac gagcctgtga agacggttag tgaagaaacc
     4381 gttgaccttg gtcatgtggt tgatgctatt aaaaaaatag aggaagaagg tcaaggtatt
     4441 tctgatgaag tcaaggctga aatcttaaaa catgaaagac atgccagcac tatcttagaa
     4501 gaaactggtc acattgagat tttaaatgaa cttcaaatcg aggaagcgat gagggaagaa
     4561 gcaggcgctg atgaccttga aactgagcaa gatcaaactg aaaatcaaga cttgaaagag
     4621 atgggcttga aggtcgagca aagttatgat attgcccagg tggctacgga tgtgactgcc
     4681 tatgttcaag cgattgtgga tgacatggat gttgaagcta cactttcaaa tgattataac
     4741 cgtcgtagca tcaatctaca aattgacacc aacgaaccag gtcgtattat cggctaccat
     4801 ggtaaagtct tgaaggcctt gcaactgttg gctcaaaatt atctttacaa ccgctattcc
     4861 aaaaccttct acgttacaat caatgtcaat gattatgtcg aacaccgtgc agaagtcttg
     4921 cagacctatg cgcaaaaatt ggcgattcgt gttttagaag aaggacgtag tcatcaaaca
     4981 gatccaatgt cgaatagtga acgcaagatt atccaccgta ttatttcacg tatggatggc
     5041 gtgaccagtt actctgaagg tgatgagcca aatcgctatg tcgttgtaga tacagaataa
     5101 gtaaaatcag ggttgacctg attttttgct agctagagga ggttaaacta atgttgaata
     5161 agataagaga ctatttagac tttgctggtt tgcagtaccg taatcctgat aaagcgggag
     5221 cagagcgaga gaagatgctg gcattccgcc acaaaggaca agaggcccga aaggctttta
     5281 cagaactggc caaagccttt caagcaagcc atccaggatg gcaactccaa cagactagtc
     5341 agtggatgaa tcaggcccag cgtttgagac cacatttttg ggtttatcta cagagagacg
     5401 aacaagtgac agaacctatg atggccttac gtttgtatgg gacatctact gactttggaa
     5461 tttctttgga agtcagtttc atcgaacgta agaaggatga gcaaacactg ggcaagcagg
     5521 ccaaagtttt agacattcca accgttaaag ggatttatta tctaacctac tctaatggtc
     5581 aaagtcaacg gtgggaggcg aatgaagaaa agcgtcgtac tttacgcgag aaggtgagaa
     5641 gtcaagaagt tcgaaaagtt ttagtgaagg tagatgttcc tatgacagaa aattcgtctg
     5701 aagaagaaat cgtagaaggc ttattgaagt cttattctaa aattcttccc tattatctag
     5761 ctacgagaaa ataagataat ttgtaaaaca tcataaatca tacagtccaa gagtgaacag
     5821 tccgctgtgt aattcttggt ctttttgttt gcgctttcgc attatataat aaacttacaa
     5881 aaacaattca aaaggagaac aattatggaa gtcgtttcaa gtgttctaaa ttggttttct
     5941 agcaatattt tgcagaatcc cgcatttttc gtaggtttat tggtgttgat aggatatgca
     6001 cttttgaaaa aacctgccca tgacgttttt tcagggtttg ttaaagcaac agtagggtat
     6061 atgttgctta acgtgggtgc tggtggtttg gttacaacct ttcgtccaat cttagcagct
     6121 cttaactaca aattccaaat tggtgcagcg gttatcgacc cttactttgg acttgctgca
     6181 gcaaacaaca aaattgcagc agagtttcca gattttgttg gaactgcaac tacagctcta
     6241 ttgattggtt ttggaataaa tatcttgctc gtagctcttc gaaagattac gaaggtaaga
     6301 accctcttta ttactggtca catcatggta caacaagctg caacagtatc tcttatggtt
     6361 ctattcttag taccacaatt gcgcaatgct tacggtacag cagcgattgg tatcatctgt
     6421 ggactttact gggcagttag ttcaaatatg actgttgagg caactcaacg cttgactggt
     6481 ggtggcggat ttgcgattgg tcaccaacag caatttgcaa tctggtttgt agataaagta
     6541 gcaggacgct ttggtaagaa agaagaaagt ttagacaatc ttaaattacc taagttcctc
     6601 tcaatcttcc acgatacagt tgttgcatct gctaccttga tgctcgtatt cttcggagcc
     6661 attcttttaa tcttgggtcc agacattatg tctaataaag aagtcatcac ttcaggaact
     6721 ctattcaatc ctgctaaaca agatttcttt atgtacatta tccaaacagc ctttaccttc
     6781 tcagtttact tgttcgtttt gatgcaaggt gtccgaatgt tcgtatctga gttgacaaac
     6841 gccttccaag gtatttcaaa caaattgttg ccaggttcat tcccagcggt tgacgttgca
     6901 gcttcttatg gatttggttc tccaaatgct gtcttgtcag gatttacctt tggtttgatt
     6961 ggtcaattga ttacaattgt tttgctcatc gtctttaaaa atccgattct tattattaca
     7021 ggatttgtac cagtgttctt tgacaatgca gccattgcgg tctacgctga taaacgcggc
     7081 ggatggaaag cggctgttat cctttccttt atatcaggtg tccttcaagt tgctctagga
     7141 gctctttgtg tggcccttct cgatttggca tcttatggtg gctaccatgg aaatatcgac
     7201 tttgaattcc catggcttgg atttggatat atcttcaaat accttggtat tgttggttat
     7261 gtacttgtgt gtctcttctt gcttgttatt cctcaacttc aatttgccaa agcaaaagat
     7321 aaagagaaat attacaacgg tgaagttcaa gaagaagctt agtatctaga aaaggagaaa
     7381 taaaatggtt aaagtattag cagcgtgcgg aaatggaatg ggttcatcaa tggttatcaa
     7441 gatgaaggtt gaaaatgctc tccgtaagct taatcaaaca gattttacag tcaattcatg
     7501 cagtgtcggt gaagctaaag gtttagcagt aggatatgac attgtaatcg cttctcttca
     7561 tttgattcaa gaattggaag ggcgaactaa tgggaagtta attgggcttg ataacttgat
     7621 ggatgataaa gaaatcaccg aaaaactcag tcaagcacta cagtaaaagg ttggaggggg
     7681 ctggacagaa actgagagtt atcgtttctg tccagccccc tctttaaata aaggaggcag
     7741 atatgaattt aaaacaagct ttaattgaca atgactcgat ccgactaggt ttagaggcta
     7801 acaattggaa agaagcagtc aaggtagcag tagatccctt gattgaaagt ggggcaattt
     7861 tgccagagta ttacgatgct atcattgaat cgactgaaga gtatgggcct tactatatct
     7921 tgatgccagg tatggctatg ccccacgcta gacctgaagc tggtgtgcaa agtgatgcct
     7981 tttcattgat taccttacaa aatcctgttg tattttcaga tgggaaagag gtatctgttt
     8041 tgttggcact agcagcaaca agttcaaaaa ttcacacaag tgtagccatt ccacaaatta
     8101 ttgccctgtt tgaattagaa gattctattg cacgtttaca ggcttgccag actaaagaag
     8161 atgtcttggc tatgattgaa gaatctaagg atagccctta tctcgaagga ttggatttgg
     8221 aaagttagaa agaggaataa agaaatgaca aaaagaatac ctaatttaca agttgcatta
     8281 gaccattcag acttgcaagg agcgattaaa gcagctgttt ctgttggtca ggaagtagat
     8341 attatcgaag ctggaactgt ttgcttgctt caagttggaa gtgaactggc tgaagtcttg
     8401 cgtagccttt tcccagataa gattattgtg gcagacacaa aatgtgctga tgctggtgga
     8461 acagttgcta aaaataatgc ggttcgtgga gcagactgga tgacttgtat ctgttgtgca
     8521 accatcccta ctatggaagc agctctaaag gctatcaaga ctgaacgagg agaacgaggc
     8581 gaaatccaga tcgagcttta tggcgattgg acttttgaac aagctcagct ttggctagat
     8641 gcaggtatct cacaagctat ttatcaccaa tctcgtgatg ctcttcttgc tggtgaaact
     8701 tggggtgaaa aagaccttaa taaggttaaa aaactcattg acatgggctt ccgtgtatct
     8761 gtaacaggtg gtctagatgt agatactctc aaactctttg aaggtgttga tgtctttacc
     8821 tttatcgcag gtcgtggaat tacagaggct gcggatccag caggagcagc gcgtgccttc
     8881 aaggatgaaa tcaaacgaat ttgggggtaa atcatggtac gtccaattgg aatttatgaa
     8941 aaggcaaccc caacacactt tacttggcta gaacgtttaa attttgccaa ggagttaggc
     9001 tttgattttg tcgagatgtc tattgacgaa cgtgacgagc gtttagcaag acttgactgg
     9061 agtaaggaag aacgcttgga agttgtcaaa gaaatctatg aaactggtgt tcgtattcct
     9121 tctatctgtt tttcaggcca tcgtcgctac ccattgggtt caaaagatcc agttctagag
     9181 gaaaaatctc tagaactcat gaaaaaatgt atcgaattag ctcaagactt gggagttcgt
     9241 acgattcaat tagctggtta cgatgtttac tatgaggaaa agtcacccca gacacgccaa
     9301 cgttttatca aaaatttgag aaaagcctgt gactgggctg aagaagctca ggtggtactt
     9361 gctattgaaa ttatggatga tcctttcatc aatagcatcg aaaaatattt ggctatagaa
     9421 aaagagattg actctccctt cctctttgta tatccagata ttggtaatgt gtctgcatgg
     9481 cataatgata tctatagtga gttttatctt ggtcatcatg ccatcgcagc tctccatctc
     9541 aaggatactt atgcagtgac agaaagttca aggggccagt tccgagatgt accttttggg
     9601 caaggttgtg tcaaatggga agaagctttc gatattttaa aggaaaccaa ttataatgga
     9661 cctttcctaa tcgaaatgtg gtctgaaaat tgtgaaacag tagaagaaac acgcgcagcc
     9721 attcaagagg cgcaagcttt tctctatcca ctcattaaga aagcaggttt gatgtaagat
     9781 gaatcaagta atcaatgcta tgcgtaaacg agtctgtgat gccaatcaat cattgccaaa
     9841 acatggactt gtcaaattta cctgggggaa tgtatctgaa gtcaatcgcg aactcggtgt
     9901 cattgttatc aaaccatcag gcgtggatta tgacgaattg acacctgaaa acatggtagt
     9961 gactgatcta gatggtaaga tcctagaagg ggatttaaga ccatcttccg acctcccaac
    10021 tcatgtgcaa ttatataagg cttggtcaga aattggtagt gtggttcaca cccattcgac
    10081 agaagctgtt gcttgggctc aggcaggtcg tgatattcct ttctacggaa caacccatgc
    10141 agattatttc tacggttcaa tcccttgcgc ccgtagtttg accaaggacg aagtagaagt
    10201 ggcctatgaa aaagatactg gcctggttat cgtagaagag tttgaacatc gcggacttaa
    10261 cccggttgaa gtaccaggaa ttgttgtacg caatcacggt ccattcacct ggggcaaaaa
    10321 tccagagaat gctgtttatc actctgtcgt actagaggaa gtatcaaaga tgaatcgctt
    10381 tacagaacaa atcaatccaa gagttggacc tgctccccag tacatactag aaaaacacta
    10441 ccaacgtaaa catggaccaa atgcttatta tggtcaaaag taagaacgat gaaggaggag
    10501 aaaaagataa atttagctcc tctttttaca tttgattttt attgagagta aagttggagt
    10561 tgaagtaatt ttaaaagatt ttttagaaat agcgcttgat atatatatgg taaaataaaa
    10621 agaattgctg tgatatcaat agatttgggg gattttttaa tatggtactg gataaggcaa
    10681 gttgtgattt gcttcaatat ttgatggatc aagaaacgtc caaaacgatt atggcgattt
    10741 cgaaagattt gaaagagtca agaaggaaaa tttattatca cattgacaaa atcaatgctg
    10801 ctctgggtga cgaggcgctt cacatcatta gtattccacg aattggtatt cacttaacgg
    10861 aagagcagag agatgcttgt tgtaaactat tatcggaagt agattcgtac gattatatca
    10921 tgagtgcgca tgaacgtatg atgataatgt tactatggat aggtatttct aaagaacgta
    10981 ttacgattga aaaattgata gagttaacag aggtatctag gaatactgtt ctcaatgatt
    11041 tgaatagtat tcgttatcaa ctaactttgg aacaatatca ggtgatcttg caagtgagca
    11101 agtcacaggg ataccacctt catgcccacc ctcttaataa aattcagtat cttcaatcgc
    11161 ttctatatca tatttttatg gaagaaaatg ccacttttgt atctatttta gaagataaga
    11221 tgaaagagag gttagatgat gagtgtttgc tttctgttga aatgaaccaa ttttttaagg
    11281 aacaggttcc tttagttgaa caagatttag ggaagaaaat aaaccatcat gaaataactt
    11341 ttatgttgca ggttctacct tatttgctgt taagctgtca taatgttgaa cagtatcaag
    11401 aaagacatca ggatatagag aaagaatttt ctttgataag aaaaagaata gagtatcagg
    11461 tgtctaagaa attaggagaa cggttgtttc aaaagtttga aatttctttg tcaggacttg
    11521 aagtttctct tgtagctgtt ctcctcctct cctatcgtaa agatttggat attcatgcag
    11581 aaagtgatga ttttcggcaa ttaaaacttg ctttagaaga atttatctgg tattttgaat
    11641 cacaaatccg aatggagatt gagaacaagg atgatttgtt acgaaatttg atgatccact
    11701 gtaaagcctt gttatttaga aagacttacg gtattttttc taaaaatcct ctaacaaaac
    11761 aaattcgatc caagtatgga gaattatttt tagtcactag aaaatctgcg gaaattttag
    11821 aaggagcatg gtttattcgg ctaacagacg atgatattgc ctatttgacg attcatattg
    11881 gaggattttt aaaatataca ccatcatctc aaaaaaatat gaaaaaagtt tatctcgttt
    11941 gtgatgaagg tgttgcggtt tcgagacttt tgctgaaaca atgcaaactt tattttccaa
    12001 atgagcaaat tgacactgta tttacaacag aacaatttaa gagtgtggaa gatattgcac
    12061 aagttgatgt agtgattact actaatgatg atttggatag cagatttccg attttaaggg
    12121 ttaatcctat ccttgaagca gaagatattt tgaaaatgct agactatctt aaacacaata
    12181 tatttcgtaa taagagcaaa agtttcagtg aaaatctttc tagtcttatt tcgtcttata
    12241 ttgtagacag caagttggct agtaagttcc aagaagaggt tcaaacactt ataaatcaag
    12301 aaatagtagt tcaagctttt ttggaagata tttgaaggac agtccaatga tgaacacaaa
    12361 cctgtgtttt tcttggtctt ttttagtgtt ttgaagggtg gtatactaat ctcaaagata
    12421 acaattatat ccaaaggagg caacatatgc caaacgtcaa agaaattaca agagagtcat
    12481 ggattttagc cactttccca gagtggggaa catggttgaa cgaagaaatc gaagaagaag
    12541 tcgtacctga aggcaacttt gccatgtggt ggctaggcaa ctgtggtact tggattaaga
    12601 caccagctgg tgctaacgtt gtcatggacc tttggtcaaa ccgtggaaaa tcaaccaaaa
    12661 aagtgaaaga tatggttcgt gggcaccaaa tggcaaatat ggcaggtgtt cgtaagctgc
    12721 aaccaaactt gcgtgttcag ccaatggtta tcgatccatt tgctatcaac gaactagact
    12781 attacttagt ttcacacttc cacagtgatc atatcgaccc atacacagct gcagcaattc
    12841 tcaataatcc taagttagag catgttaagt ttatcggtcc ttaccactgt ggacgaatct
    12901 gggaaggatg gggtgttcca aaagaacgta tcatcgttgt taaaccaggt gacactatcg
    12961 aattaaaaga tatgaagatt catgcagtag aatcatttga ccgtacttgc ttggtaactc
    13021 tcccagtgaa cggtgctgat gagacaggcg gtgaacttgc tggcttggct gttacagatg
    13081 aagaaatggc tcaaaaggct gttaactata tctttgaaac accaggtgga accatctatc
    13141 atggtgcaga ttctcacttc tcaaactatt ttgcaaaaca tggtaaagac tttaaaattg
    13201 atgttgcttt gaataactat ggtgaaaatc cggtaggtat ccaagacaaa atgacatcta
    13261 tcgaccttct tcgtatggca gaaaatctgc gtaccaaagt cattatccca gttcactatg
    13321 atatctggtc taacttcatg gcttctacta atgagattct agaactttgg aaaatgcgaa
    13381 aagatcgctt gcaatacgat ttccatccat ttatctggga agttggcggt aagtacactt
    13441 atcctcaaga tcaacactta gtagaatacc atcatccacg tggttttgat gattgttttg
    13501 aacaagactc taacattcaa tttaaagctt tgctataata taaaaatatt ctttggaggt
    13561 tatccgtatg ttgcatgata cggataattt tcatataata gtaaaataga atgtgtgatt
    13621 caataatcac ctcaaataga aaggaaattc tatgtcaaat ctatctgtta atgcaattcg
    13681 ttttctaggt attgacgcca ttaataaagc caactcaggt catccaggtg tggttatggg
    13741 agcggctccg atggcttaca gcctctttac aaaacaactt catatcaatc cagctcaacc
    13801 aaactggatt aaccgcgacc gctttattct ttcagcaggt catggttcaa tgctccttta
    13861 tgctcttctt cacctttctg gttttgaaga tgtcagcatg gatgagatta agagtttccg
    13921 tcaatggggt tcaaaaacac caggtcaccc agaatttggt catacggcag ggattgatgc
    13981 tacgacaggt cctctagggc aagggatttc aactgctact ggttttgccc aagcagaacg
    14041 tttcttggca gccaaatata accgtgaagg ttacaatatc tttgaccact atacttacgt
    14101 tatctgtgga gacggagact tgatggaagg tgtctcaagc gaggcagctt catacgcagg
    14161 cttgcaaaaa cttgataagt tggttgttct ttatgattca aatgatatca acttggatgg
    14221 tgagacaaag gattccttta cagaaagtgt tcgtgaccgt tacaatgcct acggttggca
    14281 tactgccttg gttgaaaatg gaacagactt ggaagccatc catgctgcta tcgaaacagc
    14341 aaaagcttca ggcaagccat ctttgattga agtgaagacg gttattggat acggttctcc
    14401 aaacaaacaa ggaactaatg ctgtacacgg cgcccctctt ggagcagatg aaactgcatc
    14461 aactcgtcaa gccctcggtt gggactacga accatttgaa attccagaac aagtatatgc
    14521 tgatttcaaa gaacatgttg cagaccgtgg cgcatcagct tatcaagctt ggactaaatt
    14581 agttacagat tataaagaag ctcatccaga actggctgca gaagtagaag ccatcatcga
    14641 cggacgtgat ccagtcgaag tgactccagc agacttccca gctttagaaa atggtttttc
    14701 tcaagcaact cgtaattcaa gtcaagatgc cttgaatgtt gtggcagcta aattaccaac
    14761 ctttctaggt ggatcagctg accttgctca ctcaaacatg acttatatca agacggatgg
    14821 acttcaagac gatgccaatc gcttgaatcg taacattcag tttggtgttc gtgaatttgc
    14881 aatgggaaca atcttgaacg ggatggccct tcatggtgga cttcgtgtat acggtggaac
    14941 tttcttcgtc ttctctgact atgtgaaggc agctgtccgc ttgtcagcct tacaaggact
    15001 tcctgtgact tatgtcttta cccatgattc aatcgcagtt ggggaagatg gtccgactca
    15061 tgaaccagtt gagcatttag caggtcttcg tgctatgcca aatctaaatg ttttccgtcc
    15121 agcagatgcg cgtgaaacgc aagcagcttg gtaccttgca gtgacaagtg agaaaacacc
    15181 aactgccctt gtcttgacac gtcaaaattt gactgttgaa gatggaacag acttcgacaa
    15241 ggttgctaaa ggtgcttatg ttgtatatga aaatgcagcc gactttgata ccatcttgat
    15301 tgcgacaggt tcagaggtta atcttgctgt ctcagctgct aaagaattgg ctagtcaagg
    15361 cgaaaaaatc cgcgtagtca gcatgccatc tacagatgtc tttgataaac aagatgcagc
    15421 ttacaaggaa gaaattcttc caaatgcagt ccgccgtcgt gttgcagtcg aaatgggtgc
    15481 aagtcaaaac tggtacaaat atgttggtct cgatggtgcc gttctaggta ttgatacctt
    15541 cggagcctct gccccagcac caaaagtatt ggcagaatat ggctttactg tagaaaatct
    15601 tgtaaaagtt gttcgaaact tgaaataatc ctaaaaatca gggcgtaagc tctggttttt
    15661 cttaccagaa aagtaaggta caatcttgta aaagtagctg aaatttgata tagtagtcct
    15721 atgtaaaaga caaaggagaa aacaatgaat ccaaatatta cttttttaat catgcttgta
    15781 ggtatgatgg ccttgatgtt ctttatgcaa cgttctcaaa agaaacaagc tcaaaagcgt
    15841 atggaaagct taaataaact acaaaaaggc tatgaagtga ttacaatcgg tggactttat
    15901 ggaacagtcg atgaagtaga tacggagaag ggaacaatag ttcttgacgt agagggagtt
    15961 tacttgactt ttgaactagc tgctatcaag acagtattac cgctgaaaga aacagcttca
    16021 ctagaaggcg caattgaaaa ataagacggg atcacgaact cccgtttttc tataaaagaa
    16081 aggaaatggg atgaaaaaat tagtctttgt ctgtctggga aatatttgcc gtagccctat
    16141 ggccgagttt gttatgaaat caatgacaga taactacgaa atccaaagtc gagcaacttc
    16201 ctcttgggaa catggcaatc cgattcataa ggggactcag ggaatttttc aagagtatga
    16261 gattccttat gacaagaaca agacatcgct tcagattagt aaggaagatt ttgaagcctt
    16321 tgattatatt atcggaatgg acgcttcaaa tatttctgac ttacgtcaga tgtgtccagt
    16381 agactgtcaa gataagattt actcattttc atctgaaagt gttccagacc cttggtatac
    16441 aggagatttt gaagaaacct atcgacgtgt tcaagagggc tgtcaagctt ggttagaacg
    16501 tttagaaaag gagagttaag gtggaaaatc ttacgaattt ttacgaaaag tatcgtgtct
    16561 atctgactcg tccacgttta gagcttttgg cagtagttac cattgttttc tgtgctgtac
    16621 tcgtcttttt tctaaatatt ccaggaaaag gtgtcttaaa actcgataat ggaacgattg
    16681 tttatgatgg cagtcttgtc cgcggtaaaa tgaatggcca aggtaccatt accttccaaa
    16741 atggagacca atatacaggt ggcttcaaca atggagcctt caacggaaaa ggtacctttc
    16801 aatctaaaga aggctggacc tacgaaggtg attttgtaaa tggtcaggct gaaggaaaag
    16861 ggaaactaac aacagaacaa gaagtcgttt atgaaggaac ttttaaacaa ggcgtttttc
    16921 aacaaaaata aagcctcctt atcaaaggag gtattattag aatcgcaagg taagcgctta
    16981 cctgcaaatc cctttctttc caaatccctc ttccaagcaa gtttgtgaaa taaaaaatat
    17041 ttgaaataaa tttcacaaac ttcaaagata aaacctgata agaaaagaaa atgagaaaag
    17101 tttcacaaga gtttaaaaat tctttgtgaa atgtttatga aactgtttac aaagttgaaa
    17161 taatgcgtta taataaactt gtgaaaaaat taacaaagga tatcgttcct tgaaagctat
    17221 ggaggaaaat atggctgata aaaaaactgt gacaccagag gaaaagaaac tcgttgctga
    17281 aaaacacgta gatgagttgg ttcaaaaagc tctagttgcc cttgaagaaa tgcgtaaatt
    17341 ggatcaagaa caagttgact acatcgttgc caaagcatca gtagcagctt tggatgccca
    17401 cggagaattg gctttacatg cctttgaaga aacaggacgt ggtgtatttg aagataaagc
    17461 aactaagaac ttgtttgctt gtgaacacgt ggtaaacaac atgcgtcaca ctaaaacagt
    17521 tggcgttatc gaagaagatg atgtaacagg attgaccctg attgctgaac cagttggtgt
    17581 tgtttgtggt atcactccaa caacaaaccc aacatcaaca gcgatcttca aatcattgat
    17641 ttcattgaag acacgtaatc caatcgtctt tgccttccat ccatcagctc aagaatcatc
    17701 agctcatgca gctcgtatcg tccgcgatgc agctatcgca gctggtgctc ctgaaaactg
    17761 tgtacagtgg atcactgaac catctatgga agcaactagt gcccttatga accacgaagg
    17821 tattgcaaca atccttgcaa caggtggtaa tgccatggtt aaagccgcat actcatgtgg
    17881 taaaccagct cttggggtag gtgccggaaa cgttccagct tatgttgaaa aatcagcaaa
    17941 cattcgtcaa gcagcacacg atatcgtcat gtctaaatca tttgataacg gtatggtctg
    18001 tgcatctgaa caagcagtta tcattgataa agaaatttac gatgaatttg tagcagagtt
    18061 caaatcttac cacacttact ttgtaaacaa aaaagaaaaa gctcttcttg aagagttctg
    18121 cttcggcgtc aaagcaaaca gcaaaaactg tgctggtgca aaattgaacg ctgacatcgt
    18181 tggtaaacca gcaacttgga ttgcagaaca agcaggattt acagttccag aaggaacaaa
    18241 cattcttgct gcagaatgta aagaagttgg cgaaaatgag ccattgactc gtgaaaaatt
    18301 gtcaccagtt attgcagttt tgaaatctga aagccgtgaa gatggtatta ctaaggctcg
    18361 tcaaatggtt gaatttaacg gtcttggaca ctcagcagct atccacacag ctgacgaaga
    18421 attgactaaa gaatttggta aagctgttaa agctattcgt gttatctgta actcaccttc
    18481 tacttttggt ggtatcgggg acgtttacaa tgccttcttg ccatcattga cacttggatg
    18541 tggttcttac ggacgcaact cagttgggga taacgttagt gccattaacc tcttgaatat
    18601 caaaaaagtc ggaagacgga gaaataacat gcaatggatg aaacttcctt caaaaacata
    18661 ctttgaacgt gattcaattc aataccttca aaaatgtcgt gacgttgaac gtgtcatgat
    18721 cgttactgac catgccatgg tagagcttgg tttccttgat cgtatcatcg aacaactgga
    18781 ccttcgtcgc aataaggttg tttaccaaat ctttgcggat gtagaaccgg atccagatat
    18841 cacaactgta aaccgtggta ctgagattat gcgtgccttc aaaccagata ccatcatcgc
    18901 actcggtggt gggtctccaa tggatgctgc caaagtaatg tggctcttct acgagcaacc
    18961 agaagtggac ttccgtgacc ttgtccaaaa attcatggat atccgtaaac gtgccttcaa
    19021 gttcccattg cttggtaaga agactaaatt catcgcgatt ccaactacat ctggtacagg
    19081 atctgaagta acaccatttg ccgttatctc tgataaagca aacaaccgta aatacccaat
    19141 cgctgactac tcattgacac caactgtggc aatcgtagat cctgctttgg tattgacagt
    19201 tccaggattt gttgctgctg atactggtat ggacgtattg actcacgcga cagaagcata
    19261 cgtatcacaa atggctagtg actacactga tggtttagca cttcaagcca ttaaattggt
    19321 ctttgaaaat ctcgaaagct cagttaagaa tgcagacttc cactcacgtg agaaaatgca
    19381 taacgcttca acaatcgctg gtatggcctt tgccaatgcc ttcctaggta tttctcactc
    19441 aatggcccat aagattggtg cgcaattcca cacaatccac ggtcgtacaa atgctatctt
    19501 gcttccatac gttatccgtt acaacggtac acgtccagct aagacagcaa catggcctaa
    19561 gtacaactac taccgtgcag atgaaaaata ccaagatatc gcacgcatgc ttggacttcc
    19621 agcttctact ccagaagaag gggttgaatc ttacgcaaaa gctgtctacg aactcggtga
    19681 acgtattggg atccaaatga attttagaga ccaaggaatt gacgaaaaag aatggaaaga
    19741 acattctcgt gaattagcct tcctggctta tgaagaccaa tgttcaccag ctaacccacg
    19801 tcttccaatg gtagaccata tgcaagaaat catcgaagat gcatactatg gctacaaaga
    19861 aagaccagga cgccgtaaat aattgtttat cagtctagaa gcaagacaaa aactcaattt
    19921 gagggaaaga tccagtaatt tttctatgat aaaaggcatc ctatcaaggt ttttgaacac
    19981 ctgataggat gcctttttat gatattgagg cctttttgcc ctttttgaaa aactagaata
    20041 gaaacaaaat atataataga ttgaaactgg aatagtacac ttctgcttct aaaacattgt
    20101 tagaattcga tttgactgtc ctgatcgatt tgtcctattc ctatttcatt ttactatata
    20161 aaaaatatag tatagtagac tgaatctaaa atagtacgaa acaattgcta aaacatttat
    20221 agaaattaat tttactttcc tgatagagtt gttcacatct tatttcaatt cactatagtt
    20281 taatttaaga gtagtattta ctaaggccca attaaaatca aagagcaaac tagaaaacga
    20341 gtgccattca gctcaaaaca ctgatttgag attgcagata agactagccc ctcattaaca
    20401 ggtttacgat aaaacgatga caaggtgtgt tgctttttga tttctaaaga gtataatgat
    20461 agatctctat aaaataagtg cgaaggaaat gagcttttat agtcctttcg ttttaaaata
    20521 ctatctcaga tgttcttata tcgacaagaa gtttttgagt cattccctca tcatacatat
    20581 taaataaata gtggctcatt caatttttta ctagaataat aagctagtat agtaaactga
    20641 aataagatat aaacaaataa attggagctt aacatctatt tccagcaatt ttttagaaac
    20701 tgcagtggac tattctaaat tcaacacatt ataaaaacta gagtaaaaga aaaggattgg
    20761 atcttgtgta atgcaggatc taatcctttc aatcattttg tccaactttt ggaggttcct
    20821 acaatgtagc cgtcattaat aaagacagat gggaacgaca gtgttcctat ttattttgat
    20881 agagatcgat gaattcttta gatagcaact gaataatctc tgttgaagcc atttggtctt
    20941 ctgcatgcat aaatagcaag gagaatccta ttttttctcc agtagcttct ttttgtatga
    21001 gattagagtg aatcttgtgc gcttctacta aggagtcttc cgcttcttca actttaattt
    21061 tcgcttcttt taaatttcct gccttagcta gttggatggc ttcaataaag gatgatttgg
    21121 ctgctccact attggcaatg agctgaaaac agatatattc catttcttct gtcatcttat
    21181 ttctcctatc catgcaagtg cttgttccag aacttttgct ccattcatca ttccgtaatc
    21241 ccgcatatca atggtatcta cagggatatt tcctgcaatt tctttcacag caagtaactc
    21301 ataacgaatt tgtggcccaa ttagaatgac atctgcttca tggatattct ttttcgcttc
    21361 tgtcattgat tttgcttgga tagaaatttc aatcccacgt tcagtcgcac tttgttgcat
    21421 ttttttaaca agcatacttg tcgacattcc cgcattacat actaataaaa tttgtttcat
    21481 aatcttaacc ttccatttct tgctcaacaa ctttgtcatt aactttgata aatggaatgt
    21541 atagaagaac tccaagtgca aagatgatga attgaactag aactgctctc acgtcccctg
    21601 ctgttgctaa ccatgcattt aagaatactg gtgtagtcca aggaacttgt ataaatgcag
    21661 gactcatgaa tcctgtaact gttgctaagt agctgattaa aataccaagg actggaactg
    21721 tgataaatgg aatagctaat gaaatgttat aaacgattgg gtaaccgaat aatactggtt
    21781 cattgatatt gaagatacca ggtccaaaag ataatttagc cacgttttta gaaacagcat
    21841 tgcgactcac taagaatgtt gctattaata aacataatgt agatccacta ccacccatta
    21901 aagcgaatgt ttgtatttgt gataagttga tgatgtgtgg aatggcttgt ccattatttg
    21961 ctgcagtgat gttttcagta atgttaatta atagtaatgg ttctaggatg gcactgtaaa
    22021 taactgcttg gtgaatacca aatagccata acatatttcc taaagagtaa ataataatga
    22081 ccccgattaa gcttgtacca atatgacgaa ttggttcttg aataaagatt gtaatgattg
    22141 agattaagtt cattccagtt atattgaata ataatgctga aacaatccca aataaggaga
    22201 tgacagtcat gactggaagt aatacgctaa atgatctact aacagctggt ggaatatttt
    22261 caccaaggtt catttgtaaa gctttaacgt ttgataattc aatgaataat tctgttgcaa
    22321 taatcgctac gataaccccg gcgaacattg cgcctgtacc tgtgttgttg aatgaaagaa
    22381 cacctgaaat gtttacagca tcttttgctc cgtcaggaac tacagaaact gtatttggca
    22441 tcatcacaat taaagaaact aatgatagca ttgatgctgc tgacgggttt tcgaaatctc
    22501 tgtttttagc taagaaataa ccaaccatta cagcaataat catacctgaa atacttaaag
    22561 taccgtttgc aattgttatt ccccaatatt ggaattttgt taatgtatcc ccttggaaaa
    22621 tccacttaaa taccgtgttg ttcaaaagaa cgattaaacc tgccaaaata tataatggca
    22681 ttactgttac gaatgcatct cttagggttt ttaaatgaat ttggttccct agtttaccag
    22741 caaaggtggc aaattgatct ataaattttg agtttttcat ctgaatatct cctctcttta
    22801 ctcctcaatt tctacttcaa aaccgttatt gtcacttact ttgcgatacc atcttcctga
    22861 gctcttgatg gttctctttt gcgtttctaa atcaacggag ataaatccgt aacgattctt
    22921 atatgcatta ttccaagacc agcaatcaaa tgcggtccaa gcgtggtatc caaaacagag
    22981 acttccctct tcaatggctt tatgtagcca tcttaaatgt tcttcataaa attcaatacg
    23041 atacacgtca tcgataactc cattttcatc gataaaccgt gcttcgtttt caacacccat
    23101 tccgttctca ctgataaacc atgggatatt accatattct tccttcacaa tcatagcaat
    23161 atcataaatg gctttcggaa aaatttccca accacgatat ggattcatgc gacgctccgg
    23221 ccattcatac tctttgaagt attggtctgg catccagggc gtctgatatt cctcaggatt
    23281 tgcttgtgct tgaacacgtt ttggatggta gtagtttact ccaagaaaat caaccgtatt
    23341 tgatttcatc agttgaagct ctttttcggt atgactccat aacacgccat ctctctctag
    23401 ctgttttacc aatctttctg ggaaagttcc tttaacagct ggattcaaga agactttgtt
    23461 aaagaagtct tctgtaaatc gacttgcttc taagtcttct ggagaattac ttcttggata
    23521 agcaggtgtc aagtttaaaa taatcccaat ctttccatca agtcctaatg agcgatatag
    23581 ttgaatcact tttgcactag caaggtttag attatagatg acttgtacgg cctcttttcc
    23641 ctttcctttt agatttggat aatggaaagc atataagtat cctgcttctg gaatgaccat
    23701 tggctcattg aaagttgtcc agtaatgaac cttatctcca aagcatgtga aagcagtctt
    23761 ggcaaacttc acgaataact ccactacatg tttgctttcc caaccaccgt atttttgaag
    23821 aagttccact ggtaaatcaa aatgatgtaa attcatcaca agatccatct ggttcttttt
    23881 agcttcttca atgatggcat tgtagaaagc aatacctttt ggatcaggct cacctgtctc
    23941 taaattcttg atgagtcgac tccattggat ggaagtgcgg aaagaattaa ctccaatttc
    24001 cttcatcaag tggaaatctt cggtgtaagt atgaaaaaag ttactggcta caagaggtcc
    24061 gacattatcg aaaaaatctt ctggatgcgt tttgaaccag tagtccatca cattttcatg
    24121 tacttttcca tattgacctt ctgtctgagg tccgctagaa gcagctcccc accaaaacag
    24181 attaggaaat actaactttt ttttcatgct caacctcctt tctattatgc tatacataaa
    24241 gtatagcata attttaaaat ttgtctagac atttttaaaa attgttgata catatattgt
    24301 gtttgcctca tttcttgaac tttgttatac tactattaga atattataga gagaaccaga
    24361 ttaaatgagt acaaaatatt tatttattta caatgagatt cgtgaaaaga ttctctgtaa
    24421 taaatatacc atgaacgaac aattgcctga tgaaatgaca ttagctaaac agtttgcctg
    24481 tagtcgaatg acgatcaaaa aagctttaga cttgttagtt tctgagggct taatttttag
    24541 aaaacgtggg cagggaacct ttgttctctc tcgtggcagc tcaaaaagaa aattaatcgt
    24601 tccagaaaga gatatccggg gactgacaaa aatatctgaa gatgctcatt ctacaattga
    24661 ctcgaggatt attcacttca aattagaatt tgcaaatgaa tttttagcag aaaaactaca
    24721 ggtcgctttg cagagtccag tttataatat ttaccgcctg cgtattattg acggtaaacc
    24781 ttatgttctg gaacaaactt atatgagtac cgatgttatt ccaggtatta ctgaagatat
    24841 tttacaaaaa tcgatttaca attacattga aggaaagtta ggattgcata ttgccagtgc
    24901 tacaaaaatc ttgcgagctt cttctagttc agaaaatgag caacattact tgcagctcct
    24961 tccaacagaa ccggtatttg aagtagaaca agtggcttat ttggataacg gaactccgtt
    25021 tgagtactcg attagtcgtc atcgctatga tttatttgaa tttaattctt ttgcattacg
    25081 acattcctcc taggagaaaa tgtgaaaatg aagccaatct tttacaggct ctagtttaag
    25141 aaaaatttaa aacagggcaa gaaggtccca tctatgctta aatggtttct cttttctaaa
    25201 taagatggct ttaaaagagt gatcgtttta tccatcatgt tgaaaaatat cttcgtatag
    25261 cttatagagt aggtactgaa attgttcacc tgatctactt cttatagtta tttagtttta
    25321 aatagtgttt caaacattct tacactgacg agaagttttt gagtcttttc ttgtaacaca
    25381 tatagtatac tgaggttaga atagtagact gtgacttcta acaaattgct agaaatgaat
    25441 ttcaatctcc caatttattt gcttatatat tattttaata tattaaataa attctggatc
    25501 ataatcattt aaaaaaattt tattttttta tttttcatta cgaataatat agatgaaggg
    25561 gaaagagtat gaaaacagaa ctgtttcttt tgctattagt tcaaaaggag aaaaaatgaa
    25621 agtagaaaat atttcgtata gggtggatca tcgtatattg tttgataata tttcttttga
    25681 tacttcgagt tcagacgtga cattaattac tggtaaaaat ggtacaggaa agtcaacttt
    25741 actatagtgg attgaaacta gaatagtaca catctacttc taaaatattg ttagaaatcg
    25801 atttgactat cctgatctat ttgtcctgtt cttatttcat ttcactatat ctcaaattga
    25861 gtatgacgaa gtgcgctccc atgtcctggg aacgcacttt cttcatattt ttcatattct
    25921 tgaatccatc gataaagact attgggatga atttttaaag ttgaactaat catttttaca
    25981 ggatgaggtt tacagcagag agtttgaagg ctttatcaaa ggtttttctt ggcataatga
    26041 cttttcctcg tttccactta attttgtgtc tactttatta taccaagtcc acccttaagt
    26101 tagataataa atctaactta aggaagctag aaggatgaga atccaggtgg tcaagagtcc
    26161 caaacttaag ctgattggga cacccagaat aatttgcttt ttgaaggcaa ggccacgttc
    26221 ctctatattg ggaagtgaga gttgaatgag agaaccagct gatgaaaagg gtgagatatt
    26281 agtagataga gcgccaataa cggtggctgt tgtgagtaag tgaatatcaa tctggggatt
    26341 ttgagcactg atgatagcaa tgatgggaaa gagggctgga gctacaacgg atagggtgga
    26401 actaaagagt gacatcaccc cggctatcac acaaaagaac agaggtaacc agaaatgagg
    26461 aatggatgtt gtcatgaggt gccctatcag tgtgactaaa cctgacttga ccgctagaga
    26521 cattagtaag ctcatgccgc agagcatgat aattgtagcc cagggaactt tagccaaaat
    26581 ggcttcttgc ttccctaatt tgagccttaa ggcgaggcag accatgagta ttgagacaaa
    26641 gccaatatca aatgtttttc gataagtagc tatccaggcg atgtttggga aaatgagatg
    26701 caacaaggga aaaagccaaa ccaaaaccat gctgctgatc atgagcaagg tggtttgtct
    26761 ttgaaccttg ctgaggagtg gtggttggtc aatagtcaag gatgagtttg ttcttccctt
    26821 actatagtga ctgtaacagg ataataaaag caagacaatg agtgggtaga taatgctgac
    26881 gataaagata tgattgccaa gtgaaaaagc ttgctcttcc catcccattt gcttaaacag
    26941 gccttgaaag acaatgcctg agctactggt tatcaaatta gcccctcctg aagctcccca
    27001 attgacggct tgagctccaa tcaaagggtg tttgtccgct ttttgacaga gagtaatcgc
    27061 tagaggacag caaacggcca tagtagtgaa aaatccagca cctaaagcag acaaaagggt
    27121 tgccatcagg tataaaatca tgtagagggc gttagggtag gtgcgtgtgc ggtagagaat
    27181 gtgttgagcc aaaacatcaa gagtaccgtt agttattgca aagttataaa agagagagac
    27241 gctaaaaatg gtaaaaaaga gtgaggttgg ccaaaaatga agaagttctt tggggcttaa
    27301 tcccatgaga gtggttgcga tgaggtaaga aaaagcaata gccagcaggc caatattgat
    27361 tttggtgcgg taaccaattc caatggctag agcaatggcg ctaatcatta ttaaatgaat
    27421 cattgaattg tcctttcagt tagaatatgg aaataggttg gcaattttgg cgctcaaaag
    27481 accacctgtt tcctattggt acttgactat tatacacaaa aaaaatacaa ttgtcttgac
    27541 aattgtattg acacttgtta gaattaaaaa tgaggtattc aggcttgtga tttcctatgg
    27601 aagttaatag tgattgcctc taatgcttac aagtgatatt aaaaatagag gacctagtga
    27661 tgtcaatcat ttcaactgat ttaacccctt ttcaaataga tgatacattg aaagcagcct
    27721 tgcgagaaga tgttcattcc gaagattaca gtaccaatgc catttttgat catcatggcc
    27781 aagccaaggt gtcgcttttt gccaaggaag ctggtgtttt agcggggcta accgtttttc
    27841 aaagggtttt taccctattt gatgccgagg tgaccttcca gaatcctcat caatttaagg
    27901 atggggatcg tttgactagt ggcgatttgg ttttagaaat cataggctcg gtgagaagtc
    27961 tcttaacatg tgaacgcgtt gccttgaatt ttttacaaca tttatcaggg attgcttcga
    28021 tgacagctgc ttatgtagaa gccttaggcg atgattgcat taaggtattt gatactcgaa
    28081 aaactactcc taatttacgt ctttttgaga aatatgcagt gagagttggc ggtggctata
    28141 atcatcgctt taatttatca gatgctatcc tgctaaaaga caatcacatt gcggcagtag
    28201 gtagtgttca aaaggcaatt gctcaagcgc gtgcctatgc cccttttgtg aaaatggtcg
    28261 aggtggaagt ggaaagcctt gctgctgccg aagaagctgc ggcggcgggt gctgatatta
    28321 tcatgttgga taatatgtca ttggaacaga ttgaacaggc cattacccta attgcaggac
    28381 gttctcggat tgaatgttct ggaaatattg atatgaccac tattagccgt tttcgtggtt
    28441 tagcgattga ttacgtctcc agtggtagtt taacccatag tgctaagagt cttgattttt
    28501 ccatgaaggg gttaacctac cttgatgtct aagttgtaaa ataaactaac tttttaaagg
    28561 atgtctttcc tctagaacga gttttatgtc agatagttta aacgcctctt caa
//
LOCUS       DAVBCV010000024        25876 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBCV010000024 DAVBCV010000000
VERSION     DAVBCV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.024"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(146..397)
                     /locus_tag="VQ516_001024"
     CDS             complement(146..397)
                     /locus_tag="VQ516_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511483.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYIENVYLDSLSVKQKFKFIFDFGDEWRFEC"
     gene            complement(687..2567)
                     /locus_tag="VQ516_001025"
     CDS             complement(687..2567)
                     /locus_tag="VQ516_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2511484.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2561..3430)
                     /locus_tag="VQ516_001026"
     CDS             complement(2561..3430)
                     /locus_tag="VQ516_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2511485.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3514..6159)
                     /locus_tag="VQ516_001027"
     CDS             complement(3514..6159)
                     /locus_tag="VQ516_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2511486.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDERFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVHISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(6250..7530)
                     /locus_tag="VQ516_001028"
     CDS             complement(6250..7530)
                     /locus_tag="VQ516_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2511487.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7701..9785
                     /locus_tag="VQ516_001029"
     CDS             7701..9785
                     /locus_tag="VQ516_001029"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2511488.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGKAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(9825..11504)
                     /locus_tag="VQ516_001030"
     CDS             complement(9825..11504)
                     /locus_tag="VQ516_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2511489.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFDCRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12259..13488
                     /gene="arcA"
                     /locus_tag="VQ516_001031"
     CDS             12259..13488
                     /gene="arcA"
                     /locus_tag="VQ516_001031"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2511490.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELILSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGNLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13546..14562
                     /gene="argF"
                     /locus_tag="VQ516_001032"
     CDS             13546..14562
                     /gene="argF"
                     /locus_tag="VQ516_001032"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2511491.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14727..15674
                     /gene="arcC"
                     /locus_tag="VQ516_001033"
     CDS             14727..15674
                     /gene="arcC"
                     /locus_tag="VQ516_001033"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2511492.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVIVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15883..17394
                     /locus_tag="VQ516_001034"
     CDS             15883..17394
                     /locus_tag="VQ516_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2511493.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17416..18747
                     /locus_tag="VQ516_001035"
     CDS             17416..18747
                     /locus_tag="VQ516_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2511494.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAEDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(19153..19443)
                     /locus_tag="VQ516_001036"
     CDS             complement(19153..19443)
                     /locus_tag="VQ516_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511495.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(19431..19682)
                     /locus_tag="VQ516_001037"
     CDS             complement(19431..19682)
                     /locus_tag="VQ516_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2511496.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            complement(20081..20965)
                     /locus_tag="VQ516_001038"
     CDS             complement(20081..20965)
                     /locus_tag="VQ516_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2511497.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(21110..22261)
                     /locus_tag="VQ516_001039"
     CDS             complement(21110..22261)
                     /locus_tag="VQ516_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2511498.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHRCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIEHENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(22407..24173)
                     /locus_tag="VQ516_001040"
     CDS             complement(22407..24173)
                     /locus_tag="VQ516_001040"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2511499.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(24230..>25876)
                     /locus_tag="VQ516_001041"
     CDS             complement(24230..>25876)
                     /locus_tag="VQ516_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2511500.1"
                     /translation="NYRTDKTAMWALSGNFDASKSWKKEELELANWISKNYSINPVDN
                     DFRTTTLTLKGHTGHKPQINISGDKNHYTYTENWDENTHVYTITVNHNGMVEMSINTE
                     GTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVDYNGDPNRAVDGNRNGNFNSGSVTHTR
                     ADNPSWWEVDLKKMDKVGLVKIYNRTDAETQRLSNFDVILYDNNRNEVAKKHVNNLSG
                     ESVSLDFKEKGARYIKVKLLTSGVPLSLAEVEVFRESDGKQSEEDIDKITEDKVVSTN
                     KVATQSSTNYEGVAALAVDGNKDGDYGHHSVTHTKADSNAWWQVDLGEEFTVSKVDIY
                     NRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDKVVDGLLSLKVPSVGAKLVKIELKSAA
                     IPLSLAEVEAYGSKRTPKKLSNIALTKETRQSSTDYNGFSRLAVDGNKNGDYGHHSVT
                     HTKGDSPSWWEIDLAQTEELEKLIIYNRTDAEIQRLSNFDIIIYDSNNHEVFKQHIDS
                     LESNNLSIDLKGLKGKKVRISLRNAGIPLSLAEVEVYTYK"
BASE COUNT         7331 a         5790 c         5047 g         7708 t
ORIGIN      
        1 tgaaatacta agcctacaca gactgatttc aattttacca ttcttcatag tcaaaaccat
       61 cataatctgg atattgttct ggcaacgttc caaccgaacg tacgagataa gcttcttcgt
      121 cctctgtctc gatttctctc agcacttagc attcaaaacg ccattcatca ccgaagtcga
      181 aaataaactt aaatttttgt ttgacactta ggctatccag atagacattt tctatgtaac
      241 gttcttcaac atcgtcacta acaaagctaa gaaagtaaga atctgcatga ctccactcaa
      301 cattatccat gaaaaatgca tgagcatgat catttgcaaa atcgaaactc cataaaatca
      361 catctgctag ttccgctaac gtttttgttt ttggaatcta tatatgtcgg tagcaacctt
      421 ttttaatttg acggaaatga tccaagcttc tacagatgct ggcttttcag cttctatttc
      481 ttgttttact aattttaact gtagcatttg aaaccagata attcttctgt ttcacgcgaa
      541 acaccctgcg ccaagcaagg tgttcttgtt tttatactca atgaaaatca aagagcaaac
      601 taggaagcta gctgcaggct gctcaaaaca ctgttttgag gttgtagatg gaagctgacg
      661 tggtttgaag agattttcga agagtattaa gtcgtataaa tcgtcgaagc tgtcgcaatg
      721 gtatgccact ggttagctgt agttgctgcg aaatccttgt ctgcgtagaa gtcagtaaat
      781 ggtaggattt caacttccag ctcgtcgatg cggtcaagct ggccagccag ataaacctcg
      841 atacggcttt ctgctcgttt gatgcgttgc aagagtccgc ccatacggat gtcaactgta
      901 tccaaaccaa agaccttgtt ttctttcagc cattggtgac taaagagggc atggaagtct
      961 tcaatttggc ttctaagttc tggtaattct tgtctggcga tttgttgtaa actttcttta
     1021 tcatccgctt ggtaggcctg acgaatgcgt cgtcccacat ctactttgct acttaaaata
     1081 gcattcaact gggcctgagt ttcaaagaga taagcatagt ttccagcttt ttctttaatg
     1141 ttagcaagcg tctcagcagc ctgagcgaag tgcggtttgt cctgttcagg tgtcatgtgt
     1201 tgatcaagaa tcggacaaag aatatcctga taaaaaacat agcggttggg attgataccg
     1261 ctgagattgc ctggtaggtc tggtaagagg ttggcaaggt caatctgcat aaaatcctca
     1321 accgttagac cagtattggt cttgaagtgc gcagacaaac catctaggtc attgcgatag
     1381 ctgagttctg cccagatttg caagcttggt aggatagaga actgggcagt ttcaccacca
     1441 ttgtctcccc aacccgttac gatgacttct ttaatctgat tggcacggca ggctttatta
     1501 gcctcgatag ccactagacg gctaaaatgg ttgtgaggtg taaagccaat ccacttccaa
     1561 gctccccctg caaatgcaag gtcatggcta atcttgtgat gattgcggaa attacggttg
     1621 tatttttcct cgctatcctg ataataatcc cagtaaacca gagtcacacg gtctttgaga
     1681 cggtctaggt agacacgagt ttcctctgga atttccacat cacggtcgta ctggccatcc
     1741 gctgacatga gtttgaagaa catatcactc cacatctggc agtggaaacc atatttgtca
     1801 gcaatatcca gcacgcgctc caagtgttgg cacatgagga gactacgatc cacaacaccg
     1861 ttcaaaatca ggtagcgtcc caaaccaacc aagtgggctt cgtccatccc gatattgacc
     1921 ttgcgagtct tcagtttaga caacgtggca aacatgccat caatcaggtc ataaaccttt
     1981 tcttcgccaa tgagaagaat gtcctctaca tcacggagct cctgcacttc cttgacaccc
     2041 catttgacaa aggccgacaa gtgggccaag gtctggatgc atggtacaaa ggtcatgtca
     2101 aactgttgag cataggcttc gatttcctgc aactcctctg ctgaataagc tccacggaaa
     2161 tagccaaagt aaggctgccc ttcaatctgg taagtgtctt ccatgtaaag ttcaaaggtt
     2221 gagtagccca tgagagccaa gacctcaatc atctgcttgg cagaagccac attcagcacc
     2281 gcatttcgag aacagtcaac catgtaagcc aaatcttcgt aagctgcttg ttcctcaatc
     2341 tctactttat cagcttctgc tagaactgtt accaacaagg ataaggcacg ataaagttgg
     2401 tgaggtttgc ggtaggtcaa ttgatagtga ccttcctcac cctggataga gatagaggct
     2461 tggtcagact gagtgacagc cacttccaca tctggtagag aaatatgacc ttttaaaacc
     2521 tcaatagctt gcgtttgttt gggactaagt cctgtaaatc ttaccattgc ttttcctcct
     2581 gtagccagtt gacaagagca ccgtagagat tggcatctgc gtgataggtg caggcctgga
     2641 taactggtgc gaccgtgtat tcttcgtagg tatcgacaaa gttatcaaca gccttcttga
     2701 catcttggat aaaatctgga ttttgactga tagagccacc cagactgatg acatctggat
     2761 cgatcagata ctggatattg agcaagcctt gcgccagatt gcggttcatg cgctcaatgg
     2821 cttcttgaca aaggatatta ccagctgcgg cctcttggta aatcttgcga ccgtcccaat
     2881 cagtatgacc agatttttca atcacgtatc gtaccatatt cccagttgat gctagttgcg
     2941 accagttatt aagtttttca gcaggggcaa gggttgtcat gtagccaaat tctccaccca
     3001 agccgtggcg acctcgatga agtctaccat tgataatcat ggctccgcca atccctgtcc
     3061 caatcacgac acaggctgca ttttcaagat ctggatgagc tagtagttca ctgagtccaa
     3121 cgcagttggc atcattttct aaatggacag gtagctgata agagctaagc gcctcatacc
     3181 aagaaaagcc atggatgtag ggcaccgcac tgaagccatc aatcacacct gtctcttgat
     3241 tgactgcacc tggaacgctc atagcaatcc cactgtaatc ctgttctgac aagcgttgat
     3301 ctagccacgc tagtaaatcc tccaagtttt caggcgttga aatacttgtc ttatccagta
     3361 ttttcccatc aggagtcaga ctggcaaact taatcccagt ccctccgata tcaatcgttg
     3421 caatggtcat tgtttttacc ataaaaaggg gcgaatcagc agttagttct tactttttcg
     3481 cccctccttt cttttgatgt ttactttttg aaattaaatt tcttcttttt tgatgaattc
     3541 tgtacgaatc tcttgtggat ccaatggtcc tgaatgaact ggatatggtc gctcaagtaa
     3601 gtcaagaatg gtttgttgat gttctgatat gtgcacattt tcttgactca tattatagta
     3661 acggaagaca tagccttctt cattttccgc cactttaaag gctgttggac aaacttgcgg
     3721 tatgctgaga acagaatggc tcaagaggct accagtcgca accacgcttc cttcctgtct
     3781 agcaagctga aggctggtaa acggtgtctg caaggctttg gcacgacgga aagctgaaaa
     3841 gcgttcttgg gcttggtggc attcaagtgc aaactcgact tcaaactcac gcaagcattg
     3901 tgcttctggc gttgggaagt agccccagtc acctagctca cctgatgcac gcaaaatggt
     3961 cacggcaatg gtgttatccc caaggatttc gtattcattc aatcccttat tggatacagt
     4021 cacccctttt tcatcgtcat acagactgac aaaagcttgt tggtgttgag gattttcagg
     4081 attttcccat gaagcagctg gtttgtttgg tcgtgtcacc acctcataga tactttcaga
     4141 atcattgctt ggacgcgtgt tatgagtctt gaccaagaga cggatacggt gatccttggc
     4201 agtgttagta aagcgagtct tgaagcggat ttgtggattg tcaacaaaga caatcaactc
     4261 agtttccaga ggaatgtttg tcaattcttc tgaccgtcca gcctcacgct tcataaactc
     4321 gatgatacct tgttgctctt cttctagctt ttcatccgca ctgacaggca cggtcaattc
     4381 atgtttgagc aagattttag catagcgatc tgtgttttcc aagacctcgt ggcccttaag
     4441 ctctgcaaag attggctctg ttccttttgg ttggaaatag atatactcgt ttccgatgtc
     4501 cccacggtct tcgaagcgga taaagtcttc ataggcttcg tgagttgtct tgtcatatac
     4561 tgtgatattg tcatccacac tcaccgttac gaatggtgta tcaatcactc cgttttggta
     4621 aataccctca cggtgttctt gttttccttc cagcaattgg aaggttgtcc aagaaagcgg
     4681 cgctaggtga actggaatgg tcacgcgcac ttgtcgagca atacgagctt ggcggaactt
     4741 gtcttttggt aaatcgtact caaaattagc tccgaggtct tcgattgtag cctctacagg
     4801 acgaccatcc aagtcctcca cacggtaact tggtaagata agagcagcca tctttttgta
     4861 gccttctgtt gggtgcaatt ccttgaaatc acaagtcgcc acatcaatca ctgtgctgac
     4921 agtatcgacc ttatcatgca agcctgtgtt aatgacagta aagagatagt cactttgagc
     4981 cttatccgta gcaattttac ccttccactc gttgagcaag ttacttttaa caaagtttcc
     5041 tacttggttg accttggcaa aacgcgtttc catctcgcgg tgaacttcgt ccacgctaca
     5101 gccacagata ctatcatgtg gcgcattctg caaaagtgtt ttccaagcat aggtcaactg
     5161 gtccttgtgg ttgtgtccac cagtgataat agtcaagggt tctaccactt gctctaggag
     5221 gttgctattt tcttggaagg cttgttttag gtaaatgcgg gatgaagaag tgttggcaag
     5281 tgtgtaccag ccatctgttt cctgactggt caactcgcct gtaacagttg ataagtgttc
     5341 aggaagcgca ccttctacag cttgaacata ttcatcaaaa gaactatgaa caaagattac
     5401 atccgggaag agttcatttg ccacacgaat ggcttcgctc agatttttct gtacaggctg
     5461 gtggtcacag ccgttcatca tcaaccattg gttggtcgaa gcgtaggcac gcacatctga
     5521 caatttttgt ttccagaagg tcaaggcctc atctttgtca actgggattt cattcccatt
     5581 actgtaccag ttggcaaaga gaatacctaa aacacgacta ccatccacac cctgccagta
     5641 catttctgaa aactgggatg taaaccgctc atcttcaagg acttggttgt caaatccaat
     5701 cggcttcaca ccacgaccaa aggctgccac gtgaatacct gatttttgaa ggatttgagg
     5761 agcttgtccc atatttccaa aggtatctgg aaagtagcca atctgggttg atttacccca
     5821 tttggcagct tcttgttgac caatcaaggt attgcggaca ttggcttcac tggagatcaa
     5881 gtagtcatcc tgcaagatgt aaaagggacc aattttaagt ttgccctcgt caatgtagcg
     5941 ttggaccttg tcgcgatttt cagggcgaat ttgtaagtag tcatcaagga caatagtttg
     6001 tccatccaag tggaaactct tgaactcagg gtcattttca aagagatcaa agagattgtc
     6061 aaacaattcc accaactgca tacgatggct ttcaaaaggc aagtaccact cacgatccca
     6121 gtgactatgt gagataatat gtacaacaac attttccatg aagtaaaacc tcattctaaa
     6181 tttaaatttt aacgttttaa atgtaaactt gtgattctga taagaatcgg ttgagctaaa
     6241 gcgagcccct tagcggatat ccaagtaatc caagaccaac tcacagaaca tcatgttagc
     6301 ccaggagaac cattcacgag agtagagggt cggatcatct acgtggaagc tttcgtgcat
     6361 gacacctgta ccaccatcgc aggcaaccag ctgatccagc aagaattttt tctctgcctt
     6421 atctcttgtt gtcaatcctt ggatagaaag ggcgattggc cagatatagc gatagaaggt
     6481 atgagaactt ccgagaccgc tagcgtattc tccttggtag aagtatggat tttcagagct
     6541 cagaatggta cggcgagtgg cttgatacac ttcatcatcg atcgaacagt agcccagata
     6601 gggcgcagct agtagacttg gtacatttgg atcatccatg atgctggcat ttcctaggcc
     6661 atctacttca aaagcgtaaa tcttttcgcc cttgctgttg gtggtgtaag cgtagttttc
     6721 gattccttct tggatttcat cctgaagacg cttggcatca gcaataacac tctggctatc
     6781 agctaggttt aatgctgcga agatttcttg cacataaccc aagactacta cagcaaacat
     6841 atttgacgga atcaagtaac tatactggca acagtcatca ctcggacgaa aagctgacca
     6901 agtcatacct gtcactgcaa agtcaggtcc aaagccatca tttaccaagg tgtcttcctt
     6961 acggtccgta tctcggacaa aacgataagg agagttcttg tggtcttgtt caactgtcca
     7021 tagatggaga atttccttag tcgctgcgac aaaagtttca tcaaactgac tagtctcgcc
     7081 agtctctttc cagaggagat aagccaactg caaaggatag caaagcgaat ccacctcata
     7141 cttgcgctcc cagatccagc cgttaaggtc tgtgtggtca gtctcgtggt gccctttcca
     7201 gttctcctca atgttgaagg agttagcata gggatccttg agtaccaagg tcatctgacg
     7261 tttgaccaaa cctgcaatgg tctgacgcag gagggcatct cttttagcta catgaaggta
     7321 gggtctgagt tgggctgtcg aatctcgaag ccacatggca ggaatatccc cagtcaagac
     7381 aaaagttgaa ccatcttcta agatttcaac cgtattgtcc aaggtatcgg tgtagcaacg
     7441 ctcaaagaca tcaacccact ctggatggtc cttggcccgc tctgctactt catctagcca
     7501 ttctctaaca atttctttcg aataaatcat cgtgcaattc cctctttcaa acaagtttca
     7561 tgttcagtat atagcttttg agacagaaaa tacatacaca aatgatagtc atttttctac
     7621 aaaatagtta tctttaaaac tccttttcta aaggccctct ttttctgata taatgtagaa
     7681 aaacagctaa aggaaagact atgaaaccac tacttgaaac catcgatacc cgctttggta
     7741 ctgctagcaa gcatgccttc tctcggggaa ataccctgcc atacacaggc gtgccttttg
     7801 ggatgaatta ctttgtgccc cagaccagtg accaggatgg atcttggttt tttgatccgc
     7861 atctgcctat ctttcagggg attcgattaa ctcaccagcc cagtccttgg attggggact
     7921 actcttggct ccttctgaca cctgtcacca gtcaactggg gggagacagc ctctttcacc
     7981 gccagtcttc ttatgatata gataaagctt gcttccagcc tcactatttg aagctctttt
     8041 ctctgcgtta tcagattgaa acccagctca caccgacttg ctatggtgct tctattcgtt
     8101 tgaatcaaaa gcaaggcaaa gccctctccc tctatcttca cgcagcagat gaactgacag
     8161 tagagcaagt agataagcgg actcttgccc tgcgacaaga aggtaaaact gaaactaaca
     8221 aaaattcgct aacgatgttc actgccctgc aaatgaacac ggatattctt gctatcagcc
     8281 aagaagctgg ggactggcga attgacttag caagtagtca aaccgagatg caactagcca
     8341 cttctttcat ctctccttct caagctctaa tcaatctacc tcaagaagat tttgatagct
     8401 gtaaaacaag tgcccaagcg gattgggaaa atctcctcca tcgttttgac attatagaga
     8461 caggagaggc tgaccgaacc ttctttgacc actgcctcta cagactcttc ctcttcccac
     8521 agacttttta tgaggttgat gagtcaggga aagccatcca catggatctg gctactggta
     8581 ctgtcaagcc tggtgtcctc tttagcaaca atggtttctg ggataccttc cgcaccacct
     8641 tccccctctt tgcccttatc ataccggagc actatcaacg ctttttagaa ggtttcctca
     8701 atagctaccg cgatactggt ttccttccaa aatggctggc tccagatgaa cggggtatga
     8761 tgccaggtac actgctagat ggcattatcg cagatagcgc ctgtaaggac atggcccccg
     8821 acctagaagg agaactcttc caagccatgc tcgaaacagc cagcaaagcc gaccctctcg
     8881 gcatcaatgg ccgccacgga ctagcccaat accaagaact aggttacctc tctaccgacc
     8941 accacgaaag tgttagccat actctagact atgcctatag tgacttttgt atcgccagct
     9001 gtgccaaaaa actagagaac atagaaatcg ctgaaaccta caaggctgct tcacaaaatt
     9061 accgccagct atttgacgct gagacaggtt acatgcgagc acgagacaat caaggaaact
     9121 ttcaccctga cttctctcct tatagttggg ggcgagacta cgctgaatgc tctgccattc
     9181 aagctacttt aggcgttctc cacgacatcc ctggcttaat ccaactgatg ggtggaaaag
     9241 aaacctttag caactatctt ttgaaagcct gtcaagatgc tcccctcttt gaaacaacag
     9301 gctatggtta cgaaattcac gaaatgagcg agatggctac tgctcctttt ggacaaatag
     9361 ccatttccaa ccagcctagt ttccacattc cttatctctt ccgctacagc gattaccctg
     9421 actacactgc ccttcttatc aagacgctcc gtcagaaagc ttttcaccca agttgggaag
     9481 cctatcctgg agatgaagac aatggtagtc tctctgcttg gtacatctgg tcagctctcg
     9541 gattttatcc aacctgtcca ggaaaaccaa gctatgatct cggaatccct ctctttgacc
     9601 atctacgagt ctacctagct aaagaaaata aatggctaga tattcatact gagcaaaacc
     9661 acagccattt taactttgtc aaagaatgcc gactggacaa aacactcgta tcaactattc
     9721 aacaccaaga cctcctaaaa gctgaacaac taactttcac cctcagctgg ctaccaaatc
     9781 actaataacc aaaagaacct ttgcatcgcg caaaggttct tcttttatga aacttggact
     9841 tgaagttgat ccacaagctg accatccacc gttaggttca gatagaaatc caaggtttta
     9901 tctcctgcga aatacaaggt tggtagcgtc agcctttttt cagtctcacc agcttgaaac
     9961 gcaaggacat aaatctcgtc ctgataggcg acaccatgga cacctgtccc cggttgaatc
    10021 gaaatcttag cctctaaaga ggagctactt tcactacgtt caatcctaaa atgaagagtc
    10081 tctccctttg ccacagctag gcttttttct gcaaatgcta gttcctgaac aacttctttt
    10141 tttgataacc caggagtttt ataaagggaa atcttggtca atacagggat agattgtgcc
    10201 tcggtaatca tcacgcgcac cttttgtgcc tctactagcg gacctcgcaa gaggcgctta
    10261 tgaccaactg tgaagcccct gccaaactcc tgccagacac catccacctc tacttgcaca
    10321 tgaaaagcag cgattcgttg ccctagcttc aaatcttctc ttaactcaat tacatcaaaa
    10381 gttttaggag aacctaaatc gagttctaac tggatgggca agtctgcatc gcttgcccaa
    10441 gagctggtct caaggccgtc tgtcaaatgg cgacaatcaa agtctgcgga aagagctgga
    10501 ccagatacct cagctcccag agccaaatct tctttataga gctcattgcg ataggtcgca
    10561 aattcataaa gtcgttcaat atcctttgca tcaaagagcc cagcttgatt cggcggaata
    10621 ttaagcaaga gtggagttcc tcgccctact gagtgaaagt agatttcgac caactcctcg
    10681 agagacttag gatcctgatc ctcatggtag aaccagcctg gacggatgga aacatctgcc
    10741 tctccgattg aaaaaatcgt gcccgaggga tccccgtgct gaagatagtt cagctctgct
    10801 tctgttccta gtttatcaag attcaccttt tgccacagtg gatcacctgc ataccctcgt
    10861 tcattgccaa tccagcggat actggtgcct tctgttgaaa aaatcaagca atcgccctgc
    10921 aggtcacgaa tggtttcaaa ccatttttca aattcataat taaccttttg cgcgccctct
    10981 cctctggcac catccatcca aacctcagcg aacttaccag cattcccata gttaggattt
    11041 gataagattt ccttcaactg agccagataa taggcattgt agtccgcttc tcggtccaca
    11101 tgatagaggg gactatgggc atcccacggt gacaggtaga cccccatatc catatcaaac
    11161 tctgtggcag cttgggatac ttcaaggagc aagtcgccct ttcctctcct ccaaggactg
    11221 accttaaccg aataatctgt gtgagctgtc ggataaagga caaagccatc gtggtgcttg
    11281 accaccaaaa tcaacttttt gaagcccgtt tccttgagca cacgaaccca ctcacgcgca
    11341 tccaactgac tcgggttaaa gcgctcagga tcctcctgtc cagtccccca ttcttggtca
    11401 taaaaggtat taggaccgaa gtggataaaa gctgctagtt catctcccag ataagctagc
    11461 tgagtctgac ttggtaacgg tccatgcggt ttgattttct tcatcgttat atatctcctt
    11521 cttttgcaac ttctcttgtt cacaaggtta tccacaggtt gtggataata aaccatgatc
    11581 taacagccag ctctttccaa ctccaactgg taggctcagg atttttctcc tcaatgttcc
    11641 ctcttcaaac agctctgtca tatggttctg ctaacacctc tcagaatgct actctccata
    11701 attattctca ggcttctttc aggtcattgt gtgaagacca ttcactctcc agacttttcc
    11761 acagattgtg aataagtgat tattctaagc tatcacctcg tcgactataa caaaaaataa
    11821 aaagagggtc aataagataa ttatctcttc ctaaatactc agaaaagatt ctataatttg
    11881 tacaattgaa caagaaatgc ttctccaaaa tctatcttca aaagaatgtt gcatcttttt
    11941 atcctttcac acaaacattt ctctttatac agatccaagt gataaccaga tgataattgg
    12001 acactttttg gtcatttgta tattttttga aaaatttttt aaaaaaattt tatatccttt
    12061 ttatagaagt ttatgcattt gtatctatgt attctatcct atgttatgta taataacata
    12121 aaattgtgct aaacatcata atttatatta ttttccgaga aattatagcc attgacatta
    12181 cctaggaatt gcgttataac atacatgtaa gcggtaccca aaatggtgcc aagtcaaaat
    12241 ttttaaggag gaaaatacat gtcttctcat ccaattcagg tcttctcaga aattgggaaa
    12301 ctgaaaaaag ttatgttgca ccgtccaggc aaggagttag aaaacttgtt gccggactat
    12361 cttgaaaggc ttctttttga tgatattcct ttcttggaag atgctcaaaa agaacatgat
    12421 gcatttgccc aagctcttcg cgatgaagga attgaggttc tctacctaga acaactcgct
    12481 gctgaatcat tgacctctcc agaaatccgc gatcaattta tcgaggaata cttagacgaa
    12541 gccaacatcc gtgatcgtca aaccaaggtt gctattcgtg aattacttca cggcatcaag
    12601 gacaaccaag aattggttga aaaaacaatg gctgggattc aaaaagttga attgccagaa
    12661 attcctgacg aagctaaaga tctaactgac ttagttgaat cagattatcc atttgcaatt
    12721 gacccgatgc caaacctcta tttcactcgc gacccatttg caacaattgg aaacgccgta
    12781 tcgcttaacc acatgtttgc agatactcgt aaccgtgaaa cactctacgg taagtatatc
    12841 ttcaaatacc acccaatcta tggcggaaaa gtggatttgg tctacaaccg tgaagaagat
    12901 acgcgtatcg aaggtggaga cgagctaatt ctttctaaag acgtccttgc agtaggtatc
    12961 tctcaacgta cagacgcagc ttctatcgaa aaacttttgg tcaacatctt caagaaaaat
    13021 gttggcttca agaaagtttt ggcctttgaa tttgctaaca accgtaaatt catgcacttg
    13081 gatactgtct tcactatggt agactatgac aagttcacta ttcacccaga aatcgaaggc
    13141 aaccttcacg tttactcagt tacttacgaa aacgaaaaac ttaaaattgt tgaagagaaa
    13201 ggtgacttag ctgaacttct tgctcaaaac cttggtgtag aaaaagttca tttgattcgt
    13261 tgcggtggtg gcaatatcgt agcagctgcg cgtgaacaat ggaacgacgg ttctaacact
    13321 ttgaccatcg cacctggtgt ggtagttgtt tatgaccgca ataccgtgac caataagatt
    13381 ttggaagaat acgggcttcg cttgattaag attcgcggaa gtgaattggt tcggggccgt
    13441 ggtggacctc gttgtatgtc tatgccattt gaacgtgaag aagtgtaatc gctgttcggt
    13501 attcgtcaat agaaaatgta aaaaatagaa agaggaaata ataaaatgac aaattcagta
    13561 ttccaaggac gcagcttctt agcagaaaaa gactttaccc gtgcagagtt agaatacctt
    13621 attggtcttt cagctcactt gaaagatttg aaaaaacgca atattcaaca ccactacctt
    13681 gctggcaaga atatcgctct cctatttgaa aaaacatcta ctcgtactcg tgcagccttt
    13741 acaactgcgg ctatcgacct tggtgctcac ccagaatacc tcggagcaaa tgatattcag
    13801 ttgggtaaaa aagaatctac tgaagatact gctaaagtat tgggacgtat gtttgacggg
    13861 attgaattcc gcggattcag ccaacgtatg gttgaagaat tggcagaatt ctcaggcgtt
    13921 ccagtatgga acggtctaac tgacgaatgg cacccaactc aaatgctcgc tgactacttg
    13981 actgttcaag aaaacttcgg tcgcttggaa ggcttgacat tggtatactg tggtgatgga
    14041 cgtaacaacg ttgccaacag cttgctcgta acaggtgcta tccttggtgt caatgttcac
    14101 atcttctcac caaaagaact cttcccagaa aaagaaatcg ttgaattggc agaaggattt
    14161 gctaaagaaa gtggcgcaca tgttctcatc actgaagatg ctgatgaagc agttaaagat
    14221 gcagacgttc tttacacaga cgtttgggta tcaatgggtg aagaagacaa attcgcagaa
    14281 cgtgtagctc ttcttaaacc ttaccaagtc aatatggact tagttaaaaa agcaggcaat
    14341 gaaaacttga tcttcctaca ctgcttgcca gcattccacg atactcacac tgtttatggt
    14401 aaagacgttg ctgaaaaatt tggtgtagaa gaaatggaag taacagacga agtcttccgc
    14461 agcaagtacg ctcgccactt cgatcaagca gaaaaccgta tgcacactat caaagctgtt
    14521 atggctgcta cacttggtaa cctttatatt cctaaagtat aattttagat aataaaccgt
    14581 ctaccaacag ctatgagggc tgcgactaat agctttagtc cggtcctctt ttatgtaatg
    14641 gtaatctatt atttcttata aaatatgtga aaaatcatta aattgaaatc taaacgcatt
    14701 ctattgagtg tgataaagga gaatttatgg caaatcgtaa aattgtagta gctttgggag
    14761 gaaatgcgat tctttcttct gacccatcag caaaggctca acaagaagct ttagttgaaa
    14821 cagctaagca tcttgtaaaa ttgattaaaa atggagatga tttgattatc actcacggta
    14881 atggacctca agttgggaat ctcttgctcc aacatttggc atcagactct gaaaagaacc
    14941 ctgccttccc actcgactca cttgtcgcta tgacagaagg tagcatcggt ttctggttga
    15001 aaaatgcttt gcaaaatgct ctcttggatg aaggcatcga aaaaaatgtt gcctctgttg
    15061 taacgcaagt tatcgtagat aaaaatgatc cagcttttgt taacttgagt aaaccaatcg
    15121 gtcctttcta ttcagaagaa gaagcaaaag cagaagccga aaagagcgga gcgactttca
    15181 aggaagatgc tggccgtggc tggcgtaagg tcgttgcctc accaaaacct gttgacatca
    15241 aagaaattga aaccatccgt actcttttaa ataatggtca agtcgtcgta gctgcaggtg
    15301 gtggcggtat tcccgtcgtc aaagaaaaca atggacattt gactggtgtc gaagcggtta
    15361 ttgataaaga cttcgcttcc caacgtttgg cagaattggt tgatgcagac ctcttcatcg
    15421 ttttgacagg tgtagattat gtatttgtta actacaacaa gccaaaccag gaaaaattgg
    15481 aacatgtgaa tgttgcccag ctggaagaat atatcaaaca agatcagttt gcaccaggta
    15541 gcatgcttcc aaaagtagaa gcagctatcg cttttgtcaa tggtcgtcca gaaggaaaag
    15601 cagttattac ttcccttgaa aatctaggcg ccttgattga atctgaaagc ggaacaatta
    15661 ttgaaaaagg ataagttgtt ttactaataa gatgtattct atttctagta tctttatatc
    15721 aaattagaaa ttattcttga aaacatgtac aatatttcaa aagatactag ttttagactt
    15781 taatatggta aaacaaatat aaatagaaag cgttttcttg aatgtttatt taagaaagta
    15841 gttggttttt tacactttgt tagacatcag gaggaaaaac aaatgagtga aaaagctaaa
    15901 aaagggttta agatgccttc atcttacacc gtattattga taatcattgc tattatggcc
    15961 gtgctaactt ggtttatccc tgcgggggcc tttatagaag gtatttacga ggctcagcct
    16021 caaaatccac aagggatttg ggatgtcctc atggcaccga ttcgggctat gctaggtact
    16081 catccagagg aaggttcgct cattaaagaa acgagcgcag cgattgatgt agccttcttc
    16141 atccttatgg ttggtggttt ccttggcatt gtcaacaaaa ctggtgctct tgacgtaggg
    16201 attgcctcta tcgtgaagaa gtataagggc cgcgaaaaaa tgttaatttt ggtattgatg
    16261 cctttgtttg ccctcggtgg tacaacttat ggtatgggtg aagaaacaat ggccttctat
    16321 ccactccttg tgccagttat gatggccgtt ggttttgata gcctgactgg tgttgcaatt
    16381 attttgctcg gttctcaaat cggctgtttg gcatctactc tgaatccatt tgcgacaggt
    16441 attgcttcag cgactgcggg agttggtaca ggggacggta tcgtacttcg tctgatcttc
    16501 tgggttacct tgactgctct tagtacttgg tttgtttacc gttatgcgga taagattcaa
    16561 aaagatccga ctaagtcact ggtttatagt actcgcaaag aagatttgaa acactttaac
    16621 gtagaagaat cttcatctgt agaatctaca cttagcagca aacaaaaatc agttctcttc
    16681 ttatttgtgt tgacattcat cttgatggta ttgagcttca ttccatggac agaccttggc
    16741 gttaccattt ttgatgactt taatacttgg ttgactggtc ttccagttat tggtaatatt
    16801 gtcggttcat ctacttctgc actaggtact tggtacttcc cagaaggcgc aatgctcttt
    16861 gcctttatgg gtatcctgat tggtgttatt tatggtctta aagaagataa gattatctct
    16921 tccttcatga atggtgctgc tgacttgctc agtgttgcct tgatcgtagc gattgctcgt
    16981 ggtattcaag ttatcatgaa cgacggtatg attaccgata caatcctcaa ctggggtaaa
    17041 gaaggcttga gcggtctatc ttcacaagtc tttatcgttg taacttatat cttctatcta
    17101 cctatgtcat tcttgatccc atcttcatct ggtcttgcca gcgcaactat gggtatcatg
    17161 gctccacttg gagaatttgt aaatgtccgt cctagcttga ttatcactgc ttaccaatct
    17221 gcttcaggtg tcttgaactt gattgcacca acatctggta ttgtgatggg agctcttgca
    17281 cttggacgta tcaacattgg tacttggtgg aaattcatgg gcaaactcgt agtcgctatt
    17341 attgtagtga ccatcgccct tcttctcctt ggaaccttcc ttccattcct ataaaatagt
    17401 gagtgaggtg attccatgaa aatagatata acaaatcaag ttaaagatga atttcttata
    17461 tcattaaaaa ccttgatttc ctatccttca gtactcaatg aaggagaaaa tggaacacct
    17521 tttggacaag caatccaaga tgtcctagaa aaaactttag agatttgtcg agacataggt
    17581 ttcactacct atcttgaccc taaaggttat tacggatatg cagaaatcgg tcagggagca
    17641 gagcttctgg ccattctctg tcatttggat gttgttccat caggtgatga agcagattgg
    17701 cagacaccgc catttgaagc aactatcaaa gacggctggg tattcggacg tggtgtccaa
    17761 gatgataaag gcccttcgct cgcagctctc tatgcagtaa aaagcttgct ggaccaaggt
    17821 attcagttca aaaagcgcgt acgctttatc tttggtaccg atgaggaaac cctctggcgc
    17881 tgcatggcac gctacaatac catcgaagaa caggccagta tgggctttgc acctgactca
    17941 tcttttcctc tgacctatgc tgaaaaaggg cttctacagg tcaaacttca tggccctgga
    18001 tcggatcaac tagagcttga agtaggaggc gcctttaacg ttgtaccaga caaggccaac
    18061 taccaaggtc ccctctatga acaggtttgt aacggtctca aagaagctgg ttatgattac
    18121 caatccactg aacaaaccgt aacggttctc ggagtgccaa agcatgctaa ggatgctagt
    18181 caaggtatca atgctgtcat ccgactagct accattcttg ctcctctcca agaacaccct
    18241 gctctcagtt ttcttgcaac acaagcaggt caagacggca caggaagaca aatctttggt
    18301 gatatagcag atgaaccttc tggtcaccta tcctttaatg tcgcaggtct catgatcaat
    18361 catgaacgtt ctgaaatccg tattgacatt cggactcctg tcttagctga caaggaagaa
    18421 ctagtagagt tgcttacaag atgtgcacaa aactaccaac tccgctacga agagtttgac
    18481 tatctagcgc ctctatacgt cgcagaagac agtaaactcg ttagcacact gatgcaaatc
    18541 taccaagaaa agactggcga taacagtcct gctatttcat ccggtggtgc cacttttgct
    18601 cgcaccatgc caaattgtgt agccttcggc gccttattcc caggagcgaa gcagacagaa
    18661 catcaggcaa atgaatgtgc cgttctagaa gatttgtacc gtgctatgga tatttatgcc
    18721 gaagccgtct atcgacttgc aacttaatca ggcaactgtt tctaccaaaa aaaatcgacc
    18781 gattaatgaa ctgcacccca aaagttagac agaataaatc taacttttgg ggtgttttat
    18841 tatgaaattg agttatgaag ataaagttca gatctatgaa ctaagaaagc aaggacaaag
    18901 cttcaaacag ctttcaaaaa gatttggtgt ggatgtttct ggtctaaagt catctgaatc
    18961 tttgagatga gctttataaa tcgctttttt cagtttttgc actggtgttt cgataaactc
    19021 aaacttttta gccgtggtat tgcctgattt tatagtatat tgaaactaga atagtacacc
    19081 tctacttcta aaacattttt agaaatcgat ttgactgtcc tgaacgattt gtcctgttct
    19141 tatttcattt tactatattt gagccacttc gtctttaacg gctttattca taagctcttg
    19201 taatttttct ttactatcaa ttacttctga ttttccgttg taatttattg taataggttt
    19261 taacttacct aatttctcga cacgctcatt aatttgatct tttttgaagg ctgcatatgt
    19321 ttttcctaag attttttcga aaatatattt atcagatagc ggtttgcctt cttcttcagc
    19381 ttggtttttg tattgatttg aaacataagg aacaaatcct tcatagtaac ctaatgctcc
    19441 cataagttca aaagcttgtt ttctaattca aaccattgca actcagattt cagcttttca
    19501 gataaatcct gctcatccaa ataatgactt gaaattagtg ctgaactcgt ttctgtatcc
    19561 tgtacaggct gatcacccat accagcaaaa aataaactcg ttcctagcaa gaccgaacaa
    19621 gctcctattg catatttcct caaagaaaaa cgctgctttc tctcaaattg aaattctttc
    19681 atcccatctc ccatcattca ttattactgt atattttgta tatcagaaat agtttgtatt
    19741 cacaaatctt tctagttatt cccttatcat tcctaattaa gggaggtaac atacaataat
    19801 ttttagttaa atgtatatct atgttttttg tttttcttaa taaacgcaat acaaaaagag
    19861 cctgttgcca agctctttgt actcaatgaa aatcaaatag caaactagga aactagccac
    19921 aggttgctca aaacaccgtt ttgaggtagt agataagact gacgaagtca gctcaaaaca
    19981 ctgttttgag gttgcagata gaactgacaa agtcagtaac atctatacgg caaggcgacg
    20041 ctgacgtggt ttgaagagat tttcgaagag tattagtcta ttatttcttc tcagcgcgaa
    20101 gggctgacaa gatttgtgtt cggatatcat ccacaccatt tggcgtattt ggtaaaaaga
    20161 tggtttgatt tcctttagag gcaaaggtat tcaaggtatc caaatactgg ttggtcaaga
    20221 ggatagacat gatttgttct tctgtcatgc caacattggc ttccttgagt tcggtgatag
    20281 actctgccaa tccatccaca atcgccttac gttgttgggc aatccccaca ccatgaaggc
    20341 ggtctttttc tgcttctgct tcagctgcag tgacaatttt aatcttgtca gcttccgcca
    20401 attcttgtgc tgcgacccgc ttacgttgcg ccgcattgat ttcattcatg gattgcttaa
    20461 cttctgcatc cggttcgact ttggtaatca aggttttcac gataatgtag ccgtaagtgg
    20521 tcatttcttc tgctacttgg tgttgaactt caagggcaat ctcatctttt ttctcaaaca
    20581 attcatccaa ggttaatttt ggaacagaag agcgaagagc atcttcgata taagatttaa
    20641 tctgagattc tggacgtatg agtttatagt aagcatctgt cacgctctgc tcgttgacac
    20701 ggtactgagt cgctacattc atcataacga acacattgtc cttggtctta gtctcaacca
    20761 caatatcact ttgcaacaag cgcaactgaa tccgtgctgc aatcgagtca atcccaaaag
    20821 gcaagcgaat atgaataccg ctattagcaa ccttttggta tttcccaaag cgttcaataa
    20881 tcgccaccga ctgctgacga accacataaa ctgtactcag tgtgactatc accaatagga
    20941 gcacacaaac aatcagaaaa atcatgaaaa atattgccat aatggaacct ccacaagtat
    21001 ttttctagta ttatagcaca tttaaagaag gctgtgccgt ttttactgcg atttttcctg
    21061 aaatgtcaat aattagaggt gaattgtcct attgtcgtcc aatctcttgc taaaataact
    21121 ctttataaaa ggcaatcgtt tcttctaagg ttggcataaa tggatttcct ggtgcgcagg
    21181 catcaatcaa ggcattctta gaaaggtatt caaagtcgaa atctttttct tcaataccaa
    21241 gttcagtcag tttcttagga atacctactg tctcagaaag cttctcaatc tcaacaatcg
    21301 cataatcggc acattcttga tctgatttac cttctacatg aagtcccaag gctttggcaa
    21361 cattgcggaa agcttctggt acacgtttag cattttcatg ttctataact ggtagcaaca
    21421 tggcacagca aacaccatgt ggcaaattat atactgcacc gagttggtga gccattgagt
    21481 gaacataacc aagaccagca ttattaaagc tcataccacc aaggaagatg gcattgacca
    21541 taccttcacg cgcttcaata tcatgtccat tttctacagc acgaggaaga tattccttga
    21601 taagctcaat agcaccaata gacagtttct tggttacatc ataagcacca ggtgttacca
    21661 aagcttcaac agcgtgagtc agagcatcca taccagtagc agcagtcaag gcttgaggtt
    21721 tggaaagcat gagttcaggg tcattgacag aaatcaaggc aaggctattc ttgtcaacca
    21781 ttaccatctt aaccttgcgt tcttcatcag taatcacata gttaatagtg atttctgctg
    21841 atgtacctgc cgttgtattg atagccacaa ccggcaagcc ttttttagca gacttatgaa
    21901 gcccttcata atcctgtggt tttccaccat ttgtagccat gatagaaata caactagctg
    21961 catcctgtgg agaacccccg ccaagactga tgataaaatc acatctatgt tcttgcaaag
    22021 cagctacccc atctgtgaca tttttacaag tagggtttgg ctctacatcg ctaaagatga
    22081 catattcgat tccttctgta tccagtggtt ttaaagtctt aggtaaaata tcactgcctt
    22141 cgatgtactt atctgtcacc aaaagtgcct ttttatagcc aagttccttg atataaggac
    22201 ccacttcatt tacaacacct tttccaataa ggttgactgc cggaacataa aatatagcca
    22261 tataattttc ctcctgcgcc tccgcgctat ctatttaaca tcattatatt actttttgta
    22321 ttcgttttca attatttgtg aattgtttaa caaatctttt taaaaggata ggagggacgt
    22381 aagttcacct cctatataaa caagttttat ttatgtagtg gtcccaacaa ctgacatgca
    22441 cgatagtctg ctgattctag atcttctgtt ccaaataagg accaattttt aggtctaaag
    22501 atatcttcct caggtacatt atgcatattg acaggaattc tcaacataga agccaaggta
    22561 atcaagtctg ctccaatgtg tccatatgtt atggctccgt gattagctcc ccaattattc
    22621 atgacgtcat agacagactt gaaagcacct tttcctgtca aacgtggagc aaaccaagta
    22681 gttggccatc ctgaatctgt acgattatct aaagtatggt gaacatcttc aggaagttca
    22741 agtgtgtaac cttctgcaat ttgtagcact ggaccaaccc cttttagaag attgagacgt
    22801 accattgtta ctggcatatc ccccttcgtc aagaaacgag ttgagaatcc tcctccacgg
    22861 aagtactcgc ggtttgctgg tgggaagtct gtattttcaa gcatagcctg tacttcactt
    22921 tcttccaact cccagaatgg tttcataaca ggtttgccat ctcgagtagc ttgacctgta
    22981 ccatccaatg tacaagaacc agagttgatt agatgtaaga agccagctgc agcacaaccc
    23041 tctaaagtat gtcccgttac acgtttaaca gcctctggac tccaataagt gcgcacatca
    23101 gcaaagattt gtggagtatt tgttaataga taattaaaga gcatagacac accatttagt
    23161 gaatcattct ctgtcgcaaa tacaaatggt tttcgaatac cattccagtc aaactgagta
    23221 ttgaggaaag tttccataaa atctccattt ggaaaatggt ctgtccactg acgttgacct
    23281 tggaaaccag ctactaaagc atggtgacca accgcttctt cctcaaaacc aagttcagca
    23341 agccttgggt taccaaccat taagtcacgt ccaatcatga acatcttaat aacaaattcc
    23401 cattgttttt ctttctcttc tttagaaaga atcaaatcct cacgatttcg gtcaacccct
    23461 tctttgatat gttcttttac ccaaaccata gcacgttcga actcttctgg gtcataaata
    23521 ccacggtcca tacggcgcgt gaactccgtc atatctaccg attcatttcg cattcctaag
    23581 tattcttgga agaaatctgg atttacaata gaaccgccaa tccccatcga aacactaccc
    23641 attgataggt aagcagtgtc tctcatcaag ccagttgcaa gagctgcacg cgcataacgt
    23701 aaaagttttt ctttgacatc ttctggaata tctgtatcat tagcttcctg aacatctcta
    23761 ccataaatcc caaaggctgg aatccctttt tgagcatgtg aagctagtac agctgcaaga
    23821 tagacagctc ctgggcgttc tgtcccatta aatccccaaa tagcatgagg aatatctgga
    23881 gacatatcca tagtttcact accataacac cagcatggtg taactgtaat tgttgcgcaa
    23941 acatttgatt ttttaaacaa ctcatgggaa gctgcagcct ctggaacacg accaatggta
    24001 gatggagaaa tcacacattc cacaggttcc ccatctggat atttcaatgt gcttgaaatc
    24061 aaatctgcca cacttttagc catgttcatt gtttgtactt caagtgattc gcgtacacct
    24121 tgacgacgac catcaatagt cggacgaatc ccaatacgtg gatgttgaat catactattt
    24181 tcctcttatt tttctgtttc tttcatacta ccgatgataa tttttaaaat tacttataag
    24241 tataaacctc tacctctgcc aaacttaaag gaattcctgc gtttctcaaa gaaattctaa
    24301 ccttttttcc cttcagccct tttaagtcta tggatagatt attgctttct aaactgtcaa
    24361 tatgttgttt aaaaacttca tgattatttg aatcatatat aataatatca aaatttgata
    24421 atctctgaat ttcagcatct gttctattat aaataattaa cttttctaat tcttcggttt
    24481 gtgctaaatc tatctcccac catgaaggag aatctccttt ggtatgagtc actgaatgat
    24541 gaccataatc tccgttttta tttccatcaa ctgctagacg agaaaaacca ttgtaatccg
    24601 ttgaactctg tcgagtttct ttggttaatg caatattaga aagtttcttc ggagttctct
    24661 ttgaaccata ggcttcaact tccgctaaac ttaacggaat agctgctgat tttaattcta
    24721 ttttgactag cttagcccct acagaaggta cttttaaaga taacaaacca tcaactactt
    24781 tatcaaaatg tcttctaaaa acttcttctc ctgatgaaga tagaaaaata acatcaaaat
    24841 tagataaacg ctgaggttcg gcatctgttc tattataaat atcaacttta gaaaccgtaa
    24901 actcttctcc cagatcgacc tgccaccaag cgttagaatc tgccttagta tgagtcaccg
    24961 aatgatgtcc gtaatctcca tctttattac catcaactgc taaagcagct acaccctcat
    25021 aattggttga actttgagta gctaccttat ttgtagagac tactttatct tctgttattt
    25081 tatctatatc ctcttcagat tgcttaccat ctgattctct aaaaacctct acttctgcta
    25141 aactcaaagg cactccactc gttagtaatt taactttaat ataccttgct cctttttctt
    25201 tgaaatctag actaacagat tcacccgaca aattattaac atgtttctta gcaacttcgt
    25261 ttctattatt gtcatataga atcacatcaa aattagatag acgttgagtc tcagcatctg
    25321 tgcgattata aattttaaca agcccaactt tatccatttt tttcaaatcg acttcccacc
    25381 aagagggatt atctgcccta gtgtgtgtta ccgaaccaga gttaaaatta ccatttctgt
    25441 taccatccac agctctatta gggtctccat tgtaatctac agaactttgt gttgttggtt
    25501 ttgcatatgc tatattcaaa ttaccatcat taaatttatc tggtgttggg aaagggactg
    25561 ggcctgtccc ttcagtattt atagacatct ctaccattcc attatgatta accgtaatgg
    25621 tataaacatg ggtattctca tcccaatttt ctgtataagt ataatgattt ttatcgccac
    25681 ttatatttat ctgaggttta tgaccagtat gcccttttaa tgtaagtgtt gttgtcctaa
    25741 agtcattatc tacaggattg atggaataat ttttgcttat ccagttcgct aactctaatt
    25801 cttctttctt ccaacttttt gatgcatcaa aatttcctga taatgcccac atagctgtct
    25861 tatctgtcct gtaatt
//
LOCUS       DAVBCV010000025        25177 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBCV010000025 DAVBCV010000000
VERSION     DAVBCV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.025"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..147
                     /locus_tag="VQ516_001042"
     CDS             <1..147
                     /locus_tag="VQ516_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein D"
                     /protein_id="HET2511501.1"
                     /translation="YYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNAN
                     GEWV"
     gene            548..1669
                     /gene="mnmA"
                     /locus_tag="VQ516_001043"
     CDS             548..1669
                     /gene="mnmA"
                     /locus_tag="VQ516_001043"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2511502.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1810..2265
                     /locus_tag="VQ516_001044"
     CDS             1810..2265
                     /locus_tag="VQ516_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2511503.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELDVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2275..4188
                     /gene="mnmG"
                     /locus_tag="VQ516_001045"
     CDS             2275..4188
                     /gene="mnmG"
                     /locus_tag="VQ516_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2511504.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            4377..4466
                     /locus_tag="VQ516_001046"
                     /pseudo
     CDS             4377..4466
                     /locus_tag="VQ516_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4612..6291)
                     /locus_tag="VQ516_001047"
     CDS             complement(4612..6291)
                     /locus_tag="VQ516_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2511505.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6293..6526)
                     /locus_tag="VQ516_001048"
     CDS             complement(6293..6526)
                     /locus_tag="VQ516_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2511506.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6908..7129
                     /locus_tag="VQ516_001049"
     CDS             6908..7129
                     /locus_tag="VQ516_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511507.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7342..7494)
                     /gene="cibB"
                     /locus_tag="VQ516_001050"
     CDS             complement(7342..7494)
                     /gene="cibB"
                     /locus_tag="VQ516_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2511508.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7496..7681)
                     /gene="cibA"
                     /locus_tag="VQ516_001051"
     CDS             complement(7496..7681)
                     /gene="cibA"
                     /locus_tag="VQ516_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2511509.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7859..8542
                     /gene="tsaB"
                     /locus_tag="VQ516_001052"
     CDS             7859..8542
                     /gene="tsaB"
                     /locus_tag="VQ516_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2511510.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8539..8976
                     /gene="rimI"
                     /locus_tag="VQ516_001053"
     CDS             8539..8976
                     /gene="rimI"
                     /locus_tag="VQ516_001053"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2511511.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8966..9976
                     /gene="tsaD"
                     /locus_tag="VQ516_001054"
     CDS             8966..9976
                     /gene="tsaD"
                     /locus_tag="VQ516_001054"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2511512.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(10022..11322)
                     /locus_tag="VQ516_001055"
                     /pseudo
     CDS             complement(10022..11322)
                     /locus_tag="VQ516_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11598..11949)
                     /locus_tag="VQ516_001056"
                     /pseudo
     CDS             complement(11598..11949)
                     /locus_tag="VQ516_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12056..12348)
                     /locus_tag="VQ516_001057"
                     /pseudo
     CDS             complement(12056..12348)
                     /locus_tag="VQ516_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12591..13797
                     /locus_tag="VQ516_001058"
                     /pseudo
     CDS             12591..13797
                     /locus_tag="VQ516_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            13790..14438
                     /locus_tag="VQ516_001059"
                     /pseudo
     CDS             13790..14438
                     /locus_tag="VQ516_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            14448..16064
                     /locus_tag="VQ516_001060"
     CDS             14448..16064
                     /locus_tag="VQ516_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511513.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLSGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            16084..16928
                     /locus_tag="VQ516_001061"
                     /pseudo
     CDS             16084..16928
                     /locus_tag="VQ516_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16996..18497
                     /locus_tag="VQ516_001062"
                     /pseudo
     CDS             16996..18497
                     /locus_tag="VQ516_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            18509..19258
                     /locus_tag="VQ516_001063"
     CDS             18509..19258
                     /locus_tag="VQ516_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2511514.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            19271..20443
                     /locus_tag="VQ516_001064"
     CDS             19271..20443
                     /locus_tag="VQ516_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2511515.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTRQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            20838..21701
                     /locus_tag="VQ516_001065"
     CDS             20838..21701
                     /locus_tag="VQ516_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2511516.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            21987..22220
                     /locus_tag="VQ516_001066"
     CDS             21987..22220
                     /locus_tag="VQ516_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511517.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            22242..22919
                     /locus_tag="VQ516_001067"
     CDS             22242..22919
                     /locus_tag="VQ516_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2511518.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            22931..23596
                     /locus_tag="VQ516_001068"
     CDS             22931..23596
                     /locus_tag="VQ516_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2511519.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            23668..24894
                     /locus_tag="VQ516_001069"
     CDS             23668..24894
                     /locus_tag="VQ516_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2511520.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALGTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         7763 a         4160 c         5267 g         7987 t
ORIGIN      
        1 tactatcttg aagcatcagg tgctatgaaa gcaagccaat ggttcaaagt atcagataaa
       61 tggtactatg tcaatggttt aggtgccctt gcagtcaaca caactgtaga tggctataaa
      121 gtcaatgcca atggtgaatg ggtttaagcc gattaaatta aatcatgtta agaacatttg
      181 acattttaat tttgaaacaa agataaggtt cgattgaata gatttatgtt cgtattcttt
      241 aggtacctat cttatgattt caggaaatgt cattaaaaaa acgactcctt ttctctaact
      301 tgaaaaatag attagagaaa atgggttgtt ttatttatta tagttatttg aatgaagata
      361 agaagaaggt atactcacat cattcacata atctgtatgt tgactataag tttaaaaaaa
      421 caatttttaa gctcttcctt gtcttctcca accaagcgtg ttataatgaa tactgctcaa
      481 gcgaccttca atcgtgaagc acacacgacc ttcaatcgtg aataaacgaa tagatgggag
      541 acttaccatg agtgataact ctaaaacacg tgttgtcgtg gggatgagtg gtggtgttga
      601 ttcgtcggtg acggctcttt tgctcaagga gcagggctac gatgtgatcg gtatcttcat
      661 gaagaactgg gatgacacag atgaaaacgg cgtctgtacg gcgaccgaag attacaagga
      721 tgtggttgcg gtggcagacc agattggcat tccctactac tctgtcaatt ttgaaaaaga
      781 gtactgggac cgcgtttttg agtatttcct agcggaatac cgtgcagggc gcacgccaaa
      841 tccggacgtt atgtgcaaca aggaaatcaa gttcaaggcc tttttggact atgccatgac
      901 cttgggggca gactatgtag cgactgggca ttatgctcga gtggcgcgtg atgaggacgg
      961 cactgttcac atgcttcgtg gcgtggacaa tggcaaggac cagacctatt tcctcagcca
     1021 actttcgcaa gaacaactcc aaaaaaccat gttccctctg ggacatttgg aaaagcctga
     1081 agtacgcaga ctagcagaag aagcaggcct ttcgactgct aagaagaaag actcgacagg
     1141 gatttgcttt atcggagaaa agaactttaa aaactttctc agcaactacc tgccagctca
     1201 gcctggtcgc atgatgactg tggatggtcg cgatatgggc gagcatgcag gtcttatgta
     1261 ctatacaatc ggtcagcgtg gcggactcgg tatcggtggg caacacggcg gtgacaatgc
     1321 cccttggttc gttgtcggaa aagatctaag caagaatatt ctctatgtag gacaaggatt
     1381 ctaccatgat tcgctcatgt caactagcct agaagccagt caagtccact ttactcgtga
     1441 aatgccagaa gagtttacgc tagaatgtac ggctaaattc cgttaccgtc agcctgactc
     1501 taaggtgacc gtccatgtca aaggagacaa ggcagaagtt atctttgcgg aaccacaacg
     1561 cgcgattaca ccaggacagg cagttgtctt ttacgatggc gaagagtgtc taggtggcgg
     1621 tttgattgac aatgcttacc gcgatggaca agtttgtcag tacatttaga ttgacaaatt
     1681 ttctcaattt gctacaataa taacagcaat agaaatgatg gtcaaagctc atggatgttg
     1741 caggcttttt tgtcctgcac ttctttgtag ttttgactgt ttttgtgtcg tttaagggaa
     1801 aggacaagaa tgactcaaca agactttcgg acaaaagtag acaatacggt ttttggtgtt
     1861 cgggctacag ccttgattgt ccaaaatcac aagcttctag ttaccaaaga caagggcaag
     1921 tattacacta ttggaggtgc gattcaagtc aatgaaagca cggaagacgc ggtagtccgt
     1981 gaagtgaagg aagaactgga tgtcaaagct caagctgggc agctagcttt tgtggttgaa
     2041 aatcgttttg aagtggacgg cgtttcctat cacaatatcg agtttcatta tctggtggat
     2101 ttgcttgaag atgccccgtt gaccatgcag gaagatgaga aaaggcagcc ctgtgagtgg
     2161 attgacttgg ataagctcca gaatatccag ctagttccag cctttttaaa aacagcccta
     2221 ccagattggg aaggccaact aagacacatt catcttgagg aataggagag aaacatgact
     2281 tatcatttta ctgaagaata cgatattatt gtaattggtg cgggacacgc tggggttgag
     2341 gcttccttgg ccgctagccg tatgggctgt aaggtcctgc ttgcgaccat caatattgaa
     2401 atgctggctt tcatgccttg taatccctct atcggtggtt ctgctaaggg gattgtcgta
     2461 cgtgaagtcg atgccctcgg tggcgagatg gccaagacca ttgacaagac ttacatccag
     2521 atgaagatgc tcaacacagg gaagggacca gccgttcgtg cccttcgtgc gcaggctgat
     2581 aaggaacttt actctaagga aatgcgcaag acagttgaaa atcaagaaaa tctgaccctt
     2641 cgtcaaacca tgattgatga gattttggtg gaagatggca aggttgtcgg tgtgcgtaca
     2701 gccacccatc aagaatatgc tgctaaggct gttattgtga cgacagggac tgctctccgt
     2761 ggggaaatta tcatcggaga cctcaagtac tcatcaggtc ctaaccacag cttggcttct
     2821 attaacctag ctgacaatct caaggaactg ggtctcgaaa tcggtcgttt caagacagga
     2881 acccctccac gtgtcaaggc ttcttctatc aattacgatg tgacggaaat tcagccagga
     2941 gacgaagtgc ctaatcattt ctcatacact tcacgtgatg aggattatgt caaagatcaa
     3001 gtgccatgct ggttgaccta taccaatggt accagtcatg agattatcca aaacaacctc
     3061 caccgtgcgc ctatgtttac aggtgtggtc aagggagtgg ggcctcgtta ctgtccgtcg
     3121 attgaagaca agattgtgcg ctttgcggac aaggaacgtc accaactctt ccttgagcca
     3181 gaagggcgca atactgagga agtctatgtt caaggacttt caaccagtct gcctgaggat
     3241 gtccagcgtg acttggttca ttccatcaaa ggtttggaaa atgcagagat gatgcggaca
     3301 ggttatgcta ttgagtatga tatggtcttg cctcatcagt tgcgtgcgac tttggaaacc
     3361 aagaaaatct caggtctctt cactgctggt cagacaaatg gaacatcagg ttatgaagaa
     3421 gctgctggcc aagggattat cgcgggtatc aatgcggctc tgaaaatcca aggtaaacct
     3481 gagttgattc taaaacgaag tgacggttat atcggggtga tgatcgacga cttggtgacc
     3541 aagggaacca ttgaacctta ccgtctcttg accagtcgtg ctgaataccg tctcattctt
     3601 cgtcatgaca atgctgatat gcgcttgact gagatgggac gcgagattgg ccttgtggat
     3661 gatgaacgct gggctcgttt tgaaatcaag aaaaatcaat ttgataatga gatgaaacgc
     3721 ctagacagta tcaaactcaa gccagtcaag gaaaccaatg ctaaggttga ggaaatgggc
     3781 ttcaagccgt tgacagatgc ggtgacagcc aaggaattcc ttcgccgtcc agaagtttct
     3841 taccaagatg tggtggcctt catcggacca gctgcagaag acttggatga caagattatc
     3901 gaattgattg aaacagaaat caagtacgaa ggctatattt ccaaagccat ggatcaggtt
     3961 gctaagatga aacgtatgga agaaaaacgc attccagcca atatcgactg ggatgacatc
     4021 gattctattg cgacggaagc tcgtcagaag ttcaaactca tcaatccaga aaccatcggc
     4081 caagccagcc gtatttcggg agtaaaccca gcagatattt ctattttgat ggtgtatctg
     4141 gaaggtaaaa atcgtagtat ttctaaaact cttcaaaaat caaaatgata cgtcgtcggc
     4201 ttcttacgaa tgagttcaaa gcttggcttt gattcatctc caacctccca tagttccccg
     4261 aactatggga gctaactcaa gttatgcatt ttcctagcct tctggtctga ttttcattga
     4321 gttttaatct caaaagaaag catagagaaa aacagctccg aagacggggc tgttttgttg
     4381 acttgtactc aatgaaaatc aaagagcaaa ctaggaagct agccgtaggt tgctcaaagc
     4441 actgctttga ggttgtagat agaactgacg aagtcagctc aaaacacagt tttgaggttg
     4501 tagatagaac tgacgaagtc agctcaaaac acagttttga ggttgtggat agaactgacg
     4561 aagtcagtaa catatatacg gcaaggcgaa gctgacgtgg tttgaagagt attattcttc
     4621 atctggtgtg aggatcatcg ggatgatgat cggttcacgt tcggtatttt catagaggaa
     4681 ggggcgaata gcgttgacaa tggcaccatt gacagattgc acgctagcat ccttattttt
     4741 cagtgcgata cgaatggcat tgaagaggat acgctggctt tggcgaatca agtctccaga
     4801 ctctctcatg tagacaaagc ctcggctgag gatatctggg ccagacagaa tcatctgcga
     4861 tttgaagtca acagttgcga ctgccagaac gacaccgtct tcagatagat cgcgacgatc
     4921 tttgaggaca gctgcgccaa tttcaccgat acgatttcca tcgacataga tatcttgggc
     4981 gttgaaatga cctgcgatac gagctgagtc agcagtaagg gcaagcacat cgccattgct
     5041 catgataaag atattgtctt tctcaacacc agtatccact gctagtccag cgtggacttt
     5101 ttgcatgcgg tattcaccgt ggacaggcat gaagtatttt ggcttaatca agcggagcat
     5161 gagtttttgc tcttgctgac caccgtgtcc agatgtatgg atattgttca ctttaccgtg
     5221 gataacttcg acaccagctt cagaaatgat gttaatcagc ttgttgacgc tagtagtgtt
     5281 tccagggatg ggactagaag agaagataac ggtatcacct ggttgtaatt gtacttgacg
     5341 gtgggttccg ttggcgatac gagagagggc tgccataggc tcaccctgac tacctgtaca
     5401 gaggataaga acttctcctg caggataatc tttgatttca tttggctcga taaaggttcc
     5461 cttaggagct ttgatgtagc caagatcgat tccgttgaca atggcctttt ccatagaacg
     5521 accaaagacc gcaatcttgc gtccagtctt aacagcagct tctgttgcct gctggagacg
     5581 gaagatattt gaggcaaagg atgcaaagat gatacgtcct tcaatacctt ggataatctt
     5641 cataatggac tgaccaacga ctttttcaga gttggtaaag gttggtactt ccgcatttgt
     5701 cgagtcagac aggagacaga gcacgccttc ttcaccaagc gcagccatac gatgcaagtc
     5761 cgcaggttct ccaactggag taaagtcgaa cttaaagtca cccgtacaga cgattttccc
     5821 ttgaggagta tgaatgacaa tccccaaagg ctctggaata gagtgagtcg ttctaaagaa
     5881 agttgccttg agatttttaa aggtcaactc ggtgttgtgg ttgatttcgt aaagtttggc
     5941 gttgcgcaag aggccgtgtt cttcgagttt cccacggatc aaagccaagg caagcggtcc
     6001 agcgtagata gggacatttg cttgcttgag taggaacgga atcccaccaa tgtggtcctc
     6061 gtgtccgtgt gtgattaaaa cagccttgac gcggtcgata ttgtctacga tgtaagagta
     6121 gtcaggaatg acatagtcga taccaagcaa gtcatcttct gggaatttaa tcccagcatc
     6181 gacgatgata atctcgtctt ggtattcaat tccgtaagtg tttttcccga tttctcctag
     6241 accaccgatg gcaaaaacac cgacttcttc aggtttaaga gtgtaggcca tattagaact
     6301 ccgtaatttc gaaggcgcca gtttcttttt cgtaatcgag caatttgtca gacaagagtt
     6361 cgatatattc gatattgtac tctgggcgat tttcttcgac aagttggcga gcagtgatac
     6421 ggccctcaag ttctgagctg gcatcgatgt ctaggtaaag cgcgcgtgtt gtttcacggc
     6481 gtgggctacg ttcttttgtt tcttgataaa aaactttgta aatcatatag ttcctttcta
     6541 tttacaggtc agcttattcc aaacttttag cagatatttg cttgatttca ttccattttg
     6601 tgtctagatt gaccttgatt cgttacaatt atttcaatta taccacaaaa tgaaaaatta
     6661 gtaaaagacg gaaacgagaa agttagggga cgatttcttc ggtaagcgtt tgcaaaaaca
     6721 aagaaaatgt gttatgttga taacggaagt gaagtttatt acttgtcata attccatata
     6781 gattttaagt caaggaggtt gccctatgta taacttattt gcttttgcta tcggagttcg
     6841 tttgctagct tttatcgttt ttgttttgcg tttatggagg aaatcttcta tttgccaaac
     6901 tggaacgatg acaaaccaag ttcttgagga aaattacctt tgtgactctc tactcgcttt
     6961 ttctcctcct cgtgacctat gtcgtttggt ttgtatttta ctttggatta aatgcttaga
     7021 tgccctgctt atgctggggt tttttgtttg gggatgtaga gatagtagat ttacgacacg
     7081 aaaattccac cccatctatc aagatgaggt ggtctttgca tttttctaaa atgatatgac
     7141 aatggaattt tcaaagctta ttatttttat gggagtcatt gttctgctca aaaatagagt
     7201 tgaagcataa aaataaaaca atacaaacag ataaatcgaa tggtaaaggg atggtagtaa
     7261 tgttcaaatc attgagaatg ctgagcaaac tgtaagagat gaagacaata aaatttatcc
     7321 ttctaaaaat gtgtttctta tctagcatga cttaccaaac tttttacgaa ggtcttttcc
     7381 aatggtataa cctgccataa ataaagcgat accaacacct acaccaaagc cacccttgat
     7441 ttcttgcaat tccttattag aaatttcacg acagttgttc aaatctttca tcatattaat
     7501 ctcctcctaa aaaataacca gctgccaaac cagaacctat aagtgctgtt ccacctgcaa
     7561 tagctttcca agatactgct actccaaaga taaccaaggg agcgagaccg ccatcaatat
     7621 ctgataattc attttcagat aaggataaaa attgattgtc aagaatgtca aaatttgtca
     7681 ttgaactttc ctcctttttt cttgttcact tttatattcg gaaaagaaaa ggaaaagtga
     7741 gcattttttt aaattatttt tagttaagtg agggcactct tgtctggggg caaagaaaat
     7801 tcccatgtgc catcttttgt agtataatag taagcagaca aaaagatagg aatgtgttat
     7861 gaaagtatta gcttttgata cgtccagcaa ggctctttct ctcgctattt tagaggataa
     7921 gcaggttctt gccgagacga cgattaatat caagaaaaat cacagtatta ccctcatgcc
     7981 agccattgat tttttgatgg agagtttgga ctggacgccc aaggatttgg accgaatcgt
     8041 ggtggcggaa gggccaggta gctacacagg cttgcgaatc gcggtagcca ctgccaagac
     8101 cttgactcat actctgaaca ttgagttagt tggtatgtct agcctcttgg ctctggtgcc
     8161 atatcaacaa gaaggcttgt tcgttccttt gatggatgcg cgtcgcaaca atgtttatgc
     8221 aggattttat gaaaatgcta aacctgtcat gccagaagcg cacctatctt ttgaagaggt
     8281 gctagaaaaa gtcaagggtg ctagtcaggt aacctttgtc ggagaagttg gcccctttgt
     8341 ggagcagatt caagaacact tgccaaggac taattataaa gaaaccttgc ctaatgcagc
     8401 caatcttgct cttttagctt gggacaagga agcagactcc ttgcatgatt ttgtgccaaa
     8461 ttacctcaaa cgagtcgagg ctgaggaaaa ctggctcaag aaccataccg agtctggtga
     8521 gtcctacatt aaacgcctat gatagaaatc aagcgaattc aacaacagcc tgacctagct
     8581 caagccatct acgctgttat ggcagctgtt tacctagtca gtccttggac tctggagcaa
     8641 atccaagcag atctgtccca agaccagact tggtatgcat tggcttatga tggggcagaa
     8701 gtgattggat ttctagctgt gcaggagaat ctttttgaag cagaagtcct gcaaatcgct
     8761 gtcaaaggag cttatcaggg tcagggaatt gcgtcagcct tgtttgctca attgccgaca
     8821 gataaggaga ttttcctcga agtcagaaag tcaaatcaac gagcgcaagc attttacaag
     8881 aaagaaaaaa tggcggtcat cgctgagcga aaggcctact accatgaccc agtcgaggac
     8941 gccattatca tgaagagaga aatagatgaa ggatagatat attttagcat ttgagacatc
     9001 ctgtgatgag accagtgtcg ccgtcttgaa aaacgacgat gagctcttgt ccaatgtcat
     9061 tgctagtcaa attgagagtc acaaacgttt tggtggcgta gtgcccgaag tagccagtcg
     9121 tcaccatgtc gaggtcatta cagcctgtat cgaggaggca ttggcagaag cagggattac
     9181 cgaagaggac gtgacagctg ttgcggttac ctacggacca ggcttggtcg gagccttgct
     9241 agttggtttg tcagctgcca aggcctttgc ttgggctcac ggacttccac tgattcctgt
     9301 taatcacatg gctgggcacc tcatggcagc tcagagtgtg gagcctttgg agtttccctt
     9361 gctagccctc ttggtcagcg gcggacacac agagttggtt tatgtttcgg aggcaggaga
     9421 ttataagatt gttggggaaa cccgtgatga tgcggttggt gaggcttatg ataaggtcgg
     9481 ccgtgtcatg ggcttgacct atcctgcagg tcgtgagatt gacgagctgg ctcatcaggg
     9541 gcaggatatt tatgatttcc cccgtgccat gattaaggaa gataatctgg agttctcctt
     9601 ctcaggtttg aaatctgcct ttatcaatct ttatcacaat gccgagcaaa agggagaaag
     9661 cctgtctaca gaagatttgt gtgcttcctt ccaagcagca gttatggaca ttctcatggc
     9721 aaaaaccaag aaggctttgg agaaatatcc tgttaaaacc ctggttgtgg caggtggtgt
     9781 ggcagccaat aaaggtctca gagaacgcct agcagccgaa atcacagatg tcaaggttat
     9841 catcccccct ctgcgactct gcggagacaa tgcaggtatg attgcctatg ccagcgtcag
     9901 cgagtggaac aaagaaaact tcgcaggctg ggacctcaat gccaaaccaa gtcttgcctt
     9961 tgataccatg gaataaagag tggctctttg tcaagtgtag tgggtagtcg aaaagctgca
    10021 atctggagag tacgaaattc gtactctctt ttttgatgtt caaagtcatc aaaatccgtt
    10081 ttttaaaatt gttaaagttc cggaaaccaa aggctttgcg cttgataatc ttaatcaaat
    10141 tattggtcgc ttcaagttta gcgttggaat aaggcagctt aagtgcgttg ataatcttat
    10201 ctctatccct taaaaaagtc caaaagaccg tttgaaaaag cggatgaacc gttggcagtt
    10261 cttgctcaat caatccaaag aaatggtcgg cattcttctc ttgaaaatga aagagcagga
    10321 gttgatagag ttcgtagtga acctgtaatc cctcagagta agaaagcagc ttatctcgta
    10381 cttcccgatg ggttaagtgc attcgaaacg tgggctgata gaatcgtttc tgacagagct
    10441 tgaagctata ctggtgtagt aaatcagtcc agtggactga tttagcccga gcctagaaac
    10501 gaaaaagcga gggttccaaa agcgcttgag cgcccgatac tccaaggatt ttcggtcaaa
    10561 ttggttcatg atttggattc gtactcggtt catagctcgg ctcagatgtt ggacaatgtg
    10621 gaagcggtca agaacaatct tcgcctttgg aaagagttgc ttagcgagcc gataataagg
    10681 gctgtacatg tccatggtga tgacctcgac cagctcccga acctctctct gatagcgttg
    10741 gaaatggttt cgaatcaccg catgagttcg cccgtctaaa attgcgagga tggatttgga
    10801 ctcaaaatct tgggcaatga agctcatttt gctctttttg aagctatact catcccaact
    10861 cataactttt ggcaacttgg tccaatcggt ttcaaactta aattccctca gttttcggat
    10921 gacggtggaa gttgagaccg ccaatctgtg ggcaatctca gtcatagact gcttttcaag
    10981 gaggagctga gcgatttttt gtctcaccat gttggaaatc tggcaatttt tcttgacaat
    11041 ggatgtttga gaaacgacca ccttaaggca attcttgcac tgaaagcggc gctttttgag
    11101 atgcagtacc gtgggtaaac cctgacagtc gagaagcgga attttagagg ctttctggaa
    11161 gtcgtatttg atcatcttcc cttgacaatg aggacaagga ggagcggggg aatccaactt
    11221 tgcctgaacg actagatggg tttggtattt cagaacagac aagattttga tatttttgtc
    11281 ttccaatccg agcaaatctg tggtattctt aagttgttcc atatggaccc tttctaatga
    11341 gttggttaga cgcttttcat tataggtcat atggggcttt ttttctacaa gaaacgaccc
    11401 tataattcct ggggtgggat tacccactac agaaattata gagccaaagc attccaaagt
    11461 cttgtctgac tctggaatgt tttctgttca cagttgtttt gtaactgcct aataagtaac
    11521 cgcgctataa cgaggtatct accgagattg tcaattctcc agtatactgt tataaaaaac
    11581 aaaactggag gattttttta tgtcctccca tcgtcccatc gactgttccg tagctgccca
    11641 ataacggagt atagtcggct gaaacaagaa taggacaaaa gtgcctcaag aaaagcatcg
    11701 taatttgaca atattattct gagatgtttt tgatgtaagc ccatattttc tcaatgggat
    11761 tatactcggg tgaataggta ggaagtggta acagtctatg ccctgctcct tacacatgtt
    11821 cattctgtga aaccttgcat tgtccataat gataagggat ggtgtatcta aagtaggtag
    11881 taagaatttt tggaagcaag cttcgaaaaa gtcactcgtc atcgtatctt tataggtcat
    11941 cggagccata gtatattgaa acaaaaaagg gcattagtac ttctataaca gtatcgcaat
    12001 ctctggtgtg cgttgtctcg atcttaattc aaataattat atcttcttcc agagagacta
    12061 actgcctgct caaggctcga tcatatttga gataaaaata ggtctcaacc cctgtctcat
    12121 aaatataaat aggagtcagg tagcttaagt gattcaattc tttaaggaac cgatttactt
    12181 tttctgggtc ttgttcgcag taggtacggc tctttttaga ctatatccca tagcctttag
    12241 agcgtaatga atagttgttg gaggacagtt gaattcagca gctatttcag tcaaataagc
    12301 atcgggatga gtttcaatat agatctttaa tttatctcta tcaacttttt caattaatag
    12361 tggaagtttt cttaaagtta gtgtgttgaa acgtttttat acagtgctaa aatttgaagt
    12421 gtagtgcaga tgtatttgta atagtattat agcaattctt tggagaaggt cgagacaaga
    12481 gaaaataggt atctttgaga tataagtatg atttactata aaattattga gttattcata
    12541 aaaatttctc tatcaaatat cataatgcgt cgtttaagga gatggttaca atgcaatata
    12601 gttgtggaaa aataaatatt aatatccctg atggatatgg agacattaag gatatagtgt
    12661 tttcggctca tattatagtt aggtataata atggacattg tggaggtata gatccgcata
    12721 taattggact ttgtaaaaag cagatcagaa ggatgtctcc atatcctatt ttgataatcg
    12781 tatctagaga ttcaaaggtt attgatgatt ataaaaattt agatattgcc tatgttgatt
    12841 gtactcaatg ttcaaataat tttgaaactg ccttacacgt taaaaatatt ttaaaattat
    12901 taaaaatcca acttatacac tgtcatggct actcaactaa ttatttttta tatatgttaa
    12961 aaaaacttga caagaatgga tttggaaagg ttaagacagt gataacatgc catggatggg
    13021 ttgaatataa cttaaaaaag aaattcttga cttattttga tttttggaca tattctatgg
    13081 gagatgcttt tatttgtgta tctgaaacta tgaaaaaaag attggagagt ataataaaaa
    13141 aataagaaga tagttgcaat aaataacgga attaatgtat caaatagcga tttagatgta
    13201 gtaggtgttc aagactttaa aaaagaattt tgtattccaa ataacaaaaa aatcatttgt
    13261 tatgttggaa ggttggatcc agaaaaaagg ccggatagat tccttgaatt tgcagaaaaa
    13321 ttatttttag tcagagaaga tgtgattttt attatggctg gaaatggtag tatgtgggct
    13381 actttgaaag aaaaaatttg ccatttgaaa tgtagagata atttcaggtt acttggtgaa
    13441 atctatccag ctactatagt ttaccaaata tcggatttat tgtatattcc ttctgataca
    13501 gaaggaatac ctatgtgtgt tttagagtca atgtcacaag aaactccggt tttagctagt
    13561 aatgttggcg ggttaagtga aattattgaa catagggttg atggattttt atttgagaag
    13621 gaagatgttg agggagtgtg tgcttgtgct aattttttac tcaatgattc tgagtatttg
    13681 aaatatatag gtgagaatag taaatcaaaa ataagaaaac atttttctgt gcaaaaaatg
    13741 tttgtagaaa ccatgagagt atatgatgaa ttattagaga agagtagtca tggataggaa
    13801 tatagatcaa gaattagtaa gtattataat accaacacat aataggtatg aatctctgat
    13861 tagagctgtg aaaagttgtt tacatcaaag ttataaaaat atcgaagtta taattattga
    13921 tgataattat agtaatgtaa atttaagaaa taaaattatc catcaatttg gctataccag
    13981 tcatagaatt aagttaattt taagtaatga agatttaggt gcaactaatg ccagaaacat
    14041 aggtatcaaa aattctagag gtaagtatat atcattttta gacgatgatg atgaatatat
    14101 gccagatcga attttaaagt tgatggcttg ttttaaaaag agtagaatga agaatttagc
    14161 tttagtttat agttatggaa taataattta tccaatagga aatattttaa tttattgagt
    14221 ttcaacgaga gaatattggt tacaaagaaa tattattctt taaacataaa gcggttacta
    14281 ttaataggag acaaatgcaa ggctttaaaa gtaatcaaga aggcaagaga agaaaaaatt
    14341 tttaacgaat ttctttttcg aaatatatgt tattatatca ttttggtttt ttctattgta
    14401 tatatgacaa ctatgttcaa ttaaaattta gaaagtgaga aactattgtg tatactatta
    14461 taaaatcaaa tataaaaaaa tttagtttat taacgatatt tattgttgct ggtcaattat
    14521 tgctaattta tgcagcaact attaatgctc tggtgttgaa tgaattaatt gcgatgaatt
    14581 tagagcggtt tttgaaattg tcaatctacc aaatgattgt ctggtgtggg ataatattcc
    14641 ttgactgggt agtgaaaaat tatcaggttg aagtgatcca agagtttaat ctagagattc
    14701 gaaatagagt tgccacagac atctctaact ctacctatca agaatttcat agtaaatcat
    14761 caggaacata tctttcgtgg ctaaataatg atgttcagac tttaaatgat caggctttta
    14821 aacaactttt tttagtaata aaaggaattt ctggtactat atttgcagtt gtgactctta
    14881 atcactatca ttggtcattg actgtagcca ccttgttttc attaatgatt atgctacttg
    14941 taccaaaaat ctttgcatcg aaaatgcgag aagttagtct aaatttaact aaccaaaatg
    15001 aagctttttt aaaatctagt gagactatat tgaatggatt tgatgtgtta gcgtccttga
    15061 atcttttata tgtattgcct aagaaaatta aagaagcagg aattttatta aagatggtta
    15121 tacaaagaaa gacaactgta gaaacgttag caggcgctat tagcttcttt ctcaatattt
    15181 tttttcagat atctctcgtt tttttaacag gctatcttgc aataaaagga atagtgaaaa
    15241 ttggtactat tgaagcaata ggagcactaa caggtgttat ttttacagcg ctaggtgaat
    15301 taggaggtca attatcctct attattggta cgaagcctat ttttttaaaa ttgtattcaa
    15361 ttaatccaat tgagtcaaat aaaatgaatg atatcgaacc aaatgaggtg aatagagatt
    15421 ttccgttata tgaagcaaaa aatatttgct ataagtatgg agataaagaa atattaaaaa
    15481 acttaaattt ttgttttcaa cgtaatgaaa agtatttaat tttaggtgaa agtggaagcg
    15541 ggaaatctac attattaaaa ttattgagtg gctttttgag agattatagt ggagaattgc
    15601 gattctgcgg ggatgatata aaaaaaacct cctatttaaa tatggtttcg aatgttctat
    15661 atgtagatca aaaagcttat ttgtttgaag gtacgattag agataatatt ttattggaag
    15721 aaaattatac tgatgaagaa atactacagt ctttagagca agttggtttg agtgtaaaag
    15781 attttcctaa taacatttta gattattatg ttggtgatga tgggagatta ctgtcaggag
    15841 ggcagaaaca aaaaattact ttagctagag ggctaattag aaataagaaa atagtattaa
    15901 ttgacgaggg aacttctgct atcgatagga gaacttcgtt agcgattgaa cgtaagatat
    15961 tagatagaga ggatttgact gtcattattg ttacccatgc tccgcatccg gaacttaaac
    16021 aatattttac taagatatat caatttccaa aggattttat ttaagaatat aggggaggga
    16081 aacatgagat tctattgtga agcttatgag gtaattactg aagagaaaaa agtatacttt
    16141 aatgatattg agttagaagt taagtattcc tctgtcgaag aattgtttat aatatgtgag
    16201 aaattattag ataagaaaaa agtatcattt ttttatgtat atgagaaacc cttaaggtat
    16261 ctgttatttg actatatatt tctactagtt ctagctaaaa aaaatatccc aatcttagat
    16321 ggagtttcca ataagcaagt tgatccaacg ttgctgcatc acttttcttt ggaaattgaa
    16381 aagaatttta tcgatttttg ctataaaaat atggatctca tcctaaaaac tcaaagtata
    16441 tctttgtgtc atagagaaga gcttattatt gtagatgttt cagatgactc taaatttggt
    16501 gttttttaca agcgttttag aacactggtt gataaaaata acggaaaata tgtttgtgtt
    16561 tataatttca gtcgtgtttc agatatactg caaaactgga aaaattattg caatcgaaaa
    16621 ttttcggtta cgtttactga aagccaattt gaattattta agcttttata taatcaaaaa
    16681 aattttaaaa caatttcgtt gctatttgga aaaaaattgt tgctggagga attatctatt
    16741 atagcgatct tactaatata gaatactttt gtattttttg gtgggatagt atgtttggga
    16801 aagattctat tggaaaatac gtatatgtag aagagattag tagatgtcat tttttagata
    16861 gaaattattc tttttgttat ggtttacaag attataaaag taaacttatt aaatattttt
    16921 tagaatagtt aatctattcg agagagataa agaaatatgg aaaatacttt attgaatatc
    16981 acaggaagct tcgatatgga aactaggaat ctaattagtt attctttaac tgatatcttt
    17041 gaaacagata aaataagaat tgaacttctt ggagaaattt attataaaaa tataaagttg
    17101 gaattgcatg aattcgctgg attgtataag atttatggaa ttagcttaat aaagaatatt
    17161 accggaatgt ttttaattat aatttttgat acgaagacaa aagaattaaa aatttttcaa
    17221 gatataacga cttcatactt taatttatac tatactgttt atggaggtgt gttctatata
    17281 tagtacatct ttaaagaaag tgatgaagtt atctcatgtt cccgttacct taaataacaa
    17341 aaaaatacaa gaatttatga gaaatggatt tattttagat tcaaatactt tagtaaccga
    17401 aattaataaa ttagaatatt tctcttacat ttctgtaaat aatactcttc gtatatgtgg
    17461 tattgattat aatgattcaa acaattttac taaagaacaa gtattaaaga attgggactc
    17521 tatgctacgt gaatctatat taagagtgta tagtgaagca ggagaggcta atattacact
    17581 atcatctggt tttgattcaa attatatatt atatacatta gcgaattata cgaattcttc
    17641 aattaatgca ttttgcatag gaggagaaaa aggaattaat gagattcctg aagtaactaa
    17701 aattgcaaaa ttttatggta taaatttatt agttgatact gtaatgagcc caagatttag
    17761 aatattttcc cgatatagta tggagattgg aaggaagtct ctttgaatgt ggtgtcatac
    17821 tgcagtacta tttaggcagg ttgttgttcc aaaaagggaa cacaagtatt ctttgtggcg
    17881 agtcagcgga tgaaataatg acttttaagt atcattctgt gaactacaat caattttgta
    17941 atgataaaca gaagtcagta tattttagtt attccgacta tccattttat gttacaaatt
    18001 caattgtttt aaagaaaaat agcctacttt tgcatagttt tggcattcat cctagatatc
    18061 catataaaat gtctgagatt gttgaaatgt ctaaaaaaat atcagatcta aacgataaaa
    18121 aagaatttca caaaaagaac tgtgagttga ggtttcaaga tagtgttctt gataacataa
    18181 atagtgtccc gggtacaact catttattta gttgtttaaa tattcaaaca ttagtaaaaa
    18241 ttattttata tatttttcga tacaacagtt gtatgaaaat atttaatttt agagataaag
    18301 agcttatctt ttttaataag attgtaaatg ggctgataca aaatattgag gagaatttag
    18361 aggatgatat tgaaaggata ttgaaatatt tatatatatg tttatttaac gaaatattca
    18421 ttattaagaa taaggtgaat ttttttgatg atgtggaatt taatcagaca cttagtgagt
    18481 ttttagataa attgtagagg gaatggttat gagtgacaat actaagtata tctttttatc
    18541 tccacattta gatgacgcaa ttttttcctg tggtgattat atttcaaaat taacttcaga
    18601 aggagaaatt gtacttgtta ttactatatt ttctggttac cctttgagcc aacagctaca
    18661 gccctctgct aagcaatttc ataagttgtg taaccttggg aaatatccga ttgaagaaag
    18721 gaagaaagaa gacaggttag cttgtgaacg tctacaatgt gattttagac acttatcgta
    18781 ctatgaatgt ctctatagga aagatagaaa tggcaatttt ctataccgtc atatctatag
    18841 taaattaaaa aatgaggaca cattaaagaa tgatatcata aaagagctgt taatgcattt
    18901 agacgacaaa tgtgtcgtat attgtccgct ttcacttggt gaccatgttg atcatgtttt
    18961 tgtgaatagt attggaaggg cgcttgaatt tatgcgttat aaagtaatat attatgagga
    19021 cttcccatat gtaagtgata gtagtatggt tagttatatg ggaaaaacta aagaattaaa
    19081 aatgtatcaa gaggaacttg atgaaaaaca ttatattgat agaatatcat caatattgtg
    19141 ctataaatct cagattttga taatctggaa atcagttgaa aaacttttaa acaatattaa
    19201 agagttgtat ttaaggaatg gggcagctta ttctattaga ttttggatta agaaataagg
    19261 aagtagtttt atgaatatag cagtaattgg tttggggcat gttgggctgg cctacgcgtt
    19321 actatttgca tctaaatata aagttgttgc atatgatata gattctgtaa aaataaataa
    19381 tttaaaaaag ggcactcttc catctaaaaa tgaagagctt atgaagtttt tttgcgagaa
    19441 taacttaaat attacttttt ttgatacatt ttctgaaatt aaaaataata ttgattatta
    19501 tattattgcg cttccgacag attatgatga gaaaattggt agttttaata catatgaaat
    19561 cgaacaaacg gtatcgaaga ttctgagggt aaaacctaat ggaaagatta ttttaaagtc
    19621 aacagttccg atcggctttt caaacaaatt aaaaaggctg tttgatacaa aaaatatcat
    19681 ttttgtccct gaatttttga gagaaggttg ttctatatat gataatttat atccaagtcg
    19741 catagttgtt ggagatgaga cagttgaagg aagaaaaatt gcagagttgt tcctttcgat
    19801 tagtactcat agtactgcca atattaaaaa tgttatgtta gtttctccta ctgaagcaga
    19861 agcaattaag cttttttcta acacattctt agctctccgt gttgcttttt ttaatgaact
    19921 agattctttt gctgagagga gaagtttaaa tgctgaagtt gtaataaaag gtgtttgttt
    19981 agatccaaga attggaaatt tttataataa tccttctttt ggatttggag gatattgtct
    20041 tcccaaagat actagacaat taaaaaaaga atttatagaa ataaatgccc cagtgataga
    20101 agcgattgat atttcaaaca caaatagaaa acagttcata gttaaacaaa tattggaacg
    20161 aaagccaaag atagtaggaa tatataaatt ggggatgaaa tataattcag ataattacaa
    20221 agagtcagct attttaagta taattaatga actattgatt gtcggtataa aaattttagt
    20281 atatgaacca aatttaaacg ttagtataga taacgtaatt tttgaaaaga attttgaact
    20341 atttataaaa caaagtgatc taattgttgc aaatagatgg gataggggcc ttgaagctta
    20401 taaagataaa gtttacacaa gggggatctg gataagagat taactattga tattaaaaag
    20461 gcaataattt ataaaatttg tacaaaaaaa tgttaaattt tcacattttc atagtatact
    20521 gtttaataga aagtaggttc tttgtaaccg ctccattata aaatcctttc actcttccgg
    20581 tatactatta ggaaaaacat aactggagga tattttgagc tcacacctca tcgttccagt
    20641 ggctgttccc caagctcgtg tactgcccaa aaaagttaga cattctattt agaacgagga
    20701 ttgagttctg tattgtacaa ggctcggtcc ttttagagtc agcttaaggc tggctttttc
    20761 aatcaccaaa gtgtcagaat gttttgacaa atgaacacaa ataatgatat aatatgcaaa
    20821 agctaggagg tggtaggatg attgaaaaaa tggaactggg ggaattttac aaggaattgc
    20881 gcttggcgag aaagctcaag cagtcagatg tggcttgtgc tggactaaca gcctctcagt
    20941 tgtccaagtt tgaactagga cagtctatgc tgtctgcaga caagctgatc ctagccatcc
    21001 aagggatcaa tatgaatttt gatgagtttg ggcacaaact caacaactat caagaatctc
    21061 cacatatgcg aattggtaga agggttgtgg attgcttttc ccatcaggat atagctgctt
    21121 tagagcagct attggaggaa gtcgatcaag aacagatggc gcagacctat cgtcgtttga
    21181 atgccattgt gattaaaaat gcactccatt ccttagataa aagctacccg ctaacagagg
    21241 aggatagcga gtttttgact acctacctct acgctattga gtcttggacc tggtttgaac
    21301 tctatctttt ttgcaatact atgcccttct tgagcaatca agatctgatc tttttatcaa
    21361 cctccttact cgaaaaatcc aaggaattta aagagttagt acacaatcga tggtatatga
    21421 aaagtggtta tcttaatatc atctcagaac ttatggagcg caagctattt tcttatattc
    21481 caatctttga agctgagctg gagcgcatgc tccgtccata cgatgttttt gaaaaagtat
    21541 tgtggcagtt tttaaagaaa atgcagattt ttcttcaaac caaaggaagc aatcaaaaag
    21601 agattgaaca ctttatccaa tctctgcaag tattagaaaa tccacaattg acagcccttt
    21661 ttgaattgcg tttgcagcaa tacaaagagc ttatcgatta gttagaaatg ggaaaaagat
    21721 aataatatca gcaacgatcg tcaataatgt taaaacttgt ttcttcatat tacttccctc
    21781 ctcattagta ataactttat tataccaggg aaataatcaa atctaccaaa atcgcaaatc
    21841 tgaaatttct ataagaaaaa atatcaaata tgcgattttc taaaataagc caatttccgt
    21901 gttatactgt ccttgtaaaa catttgaagc aaatcctagg tcgcagaagt ggtttacaaa
    21961 tgaagataat tgaaggagtg taaaagatgt taaatttaca atttgcagaa acaatggaat
    22021 tgacagaagc tgagttgcaa gatgttagag gaggcaacct cgtaaattct atgggaggtg
    22081 gtggaagaag tggtatatct ggttggggcg tcccaggaat ttaccctggt tggggcaatc
    22141 aagggatgag tcctaatcgt ggagcttttg attggactat agatttagca gatggattgt
    22201 ttggccgccg tagaagatag gctcttatag gagataatag gatgaagaaa atgaaagaag
    22261 tgaaatttca cctagcaact ggcttgttga ttttgactta ttacctcatt tttaatgtca
    22321 catcagactt agattttatg gttgctttgt ctgataatat gtactatgtg ttccaagttt
    22381 tattggtact tattctggga actatagcta caattgcttt tgtcaaaagt gaacattgga
    22441 aagagtgtgg tcgtttccag tttcgttggt cttacctggg tgttttcctt ctttcttttt
    22501 tcttattgtt cgtgtgggca aatctaacaa cctatatctt tcctcgtacc cagaatggaa
    22561 gtacagtcgt agaagtagcc accagtttaa caggaattag ctattttgtt acgcgaatac
    22621 tttatactag tattatcgca cctgtatcag aagaggttgt ttgtcgtggt cttttgatga
    22681 caagtttatc taaagttaag agatactatc ttgatgttct tgtttccgct gccatatttg
    22741 gcgctatgca tgttttgcag tatggttgga taacgacaga tttcataaag tattttggaa
    22801 tgggcttgat attttgtatg atgtttcgct acacccgatc catctattgg gctatagctc
    22861 tgcatgcttc atggaatagc ttcttactca ttgttacttt attggtattt ggatattaga
    22921 aaggtgatat atgaaaaaga taatctcaca ccgctacttt attatagttt ttctactagt
    22981 tattgctgac cagaagttca gtgttttagt tttacgcagc gaccttgttg ctgggctatc
    23041 tgactttgcc tattatctgt cggatatgat gttgaatttt cttgtggttt tatttgctct
    23101 tattgctatg atttggtcgg ggaaatggca gaaaatcaat agtagaaagt ttaaaggatc
    23161 ctatcttttc tattccttcc tagctctgct tgcttttgtt gcttggaatt ttgtcacctt
    23221 ttttcttttc ccacctactc gaaatgaaat ttcttatcaa catgctgctc ctacttttac
    23281 aggagctacg gcatttttga tgtatttttt ttatcctgtg attgcaggtc ccatttttga
    23341 agacatgatt tatcgtggat tggtgatgac agctctggaa aaaggaaaga aatgggggct
    23401 agatgtactt ggttctgcta ttttatttgg ggtctcgcac attagtaatc acggttgggt
    23461 cttgacagac tttttcgtct atatgggtgg cggacttata tttgcagtct tatttagaat
    23521 gacaaagtcc atttattggc ctattgggct gcatatagtc tacaacggca ttggtcagct
    23581 tttgatgtta ctgtaatttt ggttgttagt agtatcgcgg tctatttctg gggaaagatt
    23641 cccatttgaa cttaagaagg agttgtgatg aaagtatttc ttcaaaatag agattttagg
    23701 caattaacta tcaaccagtg gatttcaacg cttggggata cgatttttta tctggccttc
    23761 ctgaattatg tggcagatgc gtcttttgcc cctttggcga ttttacttat cacgatttca
    23821 gaaacccttc cccaagttct acaaatcttt ctgggagttt tggcggattt tcaacatcat
    23881 cgtgtcttga aatacacagt cattagtttt acaaaatttt tgctttactc tatagtgtcc
    23941 ctatcgcttt cagggcagtc tttttccttg ttgctagtaa cctttatttg tctgcttaac
    24001 ctcttgtctg acacattgag ttatttttca ggcgccatgc tcactccgat tttcattaga
    24061 attattgggc aagaccatct ggcagaagct attggattta aacagtcaac tgttagttta
    24121 gtgaaaacaa tcagtaatat cctaggagga gtcttactag gcattctatc catccagttt
    24181 atttccttac tgaacgctct taccttttta atcgcatttt taggtatcct cttcataaaa
    24241 actgacctct tgaaagtaga aaaaacgatt agctatcaag aaggactctc tgtaaaatcc
    24301 ttttgccagc atttgctcca atcatcgaaa ttgatatgga atatgaataa ggtgctcttg
    24361 gttttgttta ttatctctac tagtcaagca gtgataaatg ttacggttcc tatttccact
    24421 ctatttttaa ggaaccagcc ctttttgaat ttacaaactg gtcaatctct tgccttgtta
    24481 gccacactag aattatcagc ccttattttg ggaagtctga tgagtggcta tctgcaacat
    24541 accatttcca taaaaatagc cctgtatgct tcactcctac tccaattact actcctagtg
    24601 ggctttgcga cagctcattt tggcttgatt ctgatcttta gctttttgga tgcctttttc
    24661 gcgggtattc tctctcctag attgcaagaa ctcgtcttta aacaaatacc tgaggagtca
    24721 atgggagcag ttcaatcttc tatcggtgcc attacggttg ttttacctag cttatttaca
    24781 atagctttgg gaaccattgc tactagcttt ggaactctgg cagttagctt tgttttatta
    24841 ctatttcttc tagttgcctt tgtcatgctt ctgaatattc gtgaaagtat ttagtggaga
    24901 atttctataa tatatgtttg agactggatt gccagtcttt ttatgatggt caaaatcaac
    24961 agactttttc tcttttctga atgtgttata gtagactgaa tctaaaatag tacgaaataa
    25021 ttgctaaaac atttatagaa attaatttta ctttcccaat cgatttgttc tcatcttatt
    25081 tcaatctgct atataatagt ccatgctgtg gctggattct tttcagccta cttatactct
    25141 tcgaaactct cttcaaacca cgtcagcttc accttgc
//
LOCUS       DAVBCV010000026        24327 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBCV010000026 DAVBCV010000000
VERSION     DAVBCV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.026"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..618)
                     /locus_tag="VQ516_001070"
     CDS             complement(<1..618)
                     /locus_tag="VQ516_001070"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2511521.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEK"
     gene            complement(769..2079)
                     /gene="secY"
                     /locus_tag="VQ516_001071"
     CDS             complement(769..2079)
                     /gene="secY"
                     /locus_tag="VQ516_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2511522.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(2092..2532)
                     /gene="rplO"
                     /locus_tag="VQ516_001072"
     CDS             complement(2092..2532)
                     /gene="rplO"
                     /locus_tag="VQ516_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2511523.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(2677..2901)
                     /gene="rpmD"
                     /locus_tag="VQ516_001073"
     CDS             complement(2677..2901)
                     /gene="rpmD"
                     /locus_tag="VQ516_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2511524.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(2873..3367)
                     /gene="rpsE"
                     /locus_tag="VQ516_001074"
     CDS             complement(2873..3367)
                     /gene="rpsE"
                     /locus_tag="VQ516_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2511525.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(3385..3741)
                     /gene="rplR"
                     /locus_tag="VQ516_001075"
     CDS             complement(3385..3741)
                     /gene="rplR"
                     /locus_tag="VQ516_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2511526.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(3825..4361)
                     /gene="rplF"
                     /locus_tag="VQ516_001076"
     CDS             complement(3825..4361)
                     /gene="rplF"
                     /locus_tag="VQ516_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2511527.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(4553..4951)
                     /gene="rpsH"
                     /locus_tag="VQ516_001077"
     CDS             complement(4553..4951)
                     /gene="rpsH"
                     /locus_tag="VQ516_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2511528.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(5236..5505)
                     /gene="rpsN"
                     /locus_tag="VQ516_001078"
     CDS             complement(5236..5505)
                     /gene="rpsN"
                     /locus_tag="VQ516_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2511529.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(5523..6065)
                     /gene="rplE"
                     /locus_tag="VQ516_001079"
     CDS             complement(5523..6065)
                     /gene="rplE"
                     /locus_tag="VQ516_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2511530.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(6089..6394)
                     /gene="rplX"
                     /locus_tag="VQ516_001080"
     CDS             complement(6089..6394)
                     /gene="rplX"
                     /locus_tag="VQ516_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2511531.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(6472..6840)
                     /gene="rplN"
                     /locus_tag="VQ516_001081"
     CDS             complement(6472..6840)
                     /gene="rplN"
                     /locus_tag="VQ516_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2511532.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(6866..7126)
                     /gene="rpsQ"
                     /locus_tag="VQ516_001082"
     CDS             complement(6866..7126)
                     /gene="rpsQ"
                     /locus_tag="VQ516_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2511533.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(7151..7357)
                     /locus_tag="VQ516_001083"
     CDS             complement(7151..7357)
                     /locus_tag="VQ516_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2511534.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(7367..7780)
                     /gene="rplP"
                     /locus_tag="VQ516_001084"
     CDS             complement(7367..7780)
                     /gene="rplP"
                     /locus_tag="VQ516_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2511535.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(7784..8437)
                     /gene="rpsC"
                     /locus_tag="VQ516_001085"
     CDS             complement(7784..8437)
                     /gene="rpsC"
                     /locus_tag="VQ516_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2511536.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(8450..8794)
                     /gene="rplV"
                     /locus_tag="VQ516_001086"
     CDS             complement(8450..8794)
                     /gene="rplV"
                     /locus_tag="VQ516_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2511537.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(8806..9087)
                     /gene="rpsS"
                     /locus_tag="VQ516_001087"
     CDS             complement(8806..9087)
                     /gene="rpsS"
                     /locus_tag="VQ516_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2511538.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(9191..10024)
                     /gene="rplB"
                     /locus_tag="VQ516_001088"
     CDS             complement(9191..10024)
                     /gene="rplB"
                     /locus_tag="VQ516_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2511539.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(10042..10338)
                     /locus_tag="VQ516_001089"
     CDS             complement(10042..10338)
                     /locus_tag="VQ516_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2511540.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(10338..10961)
                     /gene="rplD"
                     /locus_tag="VQ516_001090"
     CDS             complement(10338..10961)
                     /gene="rplD"
                     /locus_tag="VQ516_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2511541.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(10986..11612)
                     /gene="rplC"
                     /locus_tag="VQ516_001091"
     CDS             complement(10986..11612)
                     /gene="rplC"
                     /locus_tag="VQ516_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2511542.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(11828..12136)
                     /gene="rpsJ"
                     /locus_tag="VQ516_001092"
     CDS             complement(11828..12136)
                     /gene="rpsJ"
                     /locus_tag="VQ516_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2511543.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(12402..13019)
                     /locus_tag="VQ516_001093"
     CDS             complement(12402..13019)
                     /locus_tag="VQ516_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2511544.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(13016..13606)
                     /gene="nrdG"
                     /locus_tag="VQ516_001094"
     CDS             complement(13016..13606)
                     /gene="nrdG"
                     /locus_tag="VQ516_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2511545.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKEIYEQVKRE"
     gene            complement(13599..14105)
                     /locus_tag="VQ516_001095"
     CDS             complement(13599..14105)
                     /locus_tag="VQ516_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511546.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(14149..14289)
                     /locus_tag="VQ516_001096"
     CDS             complement(14149..14289)
                     /locus_tag="VQ516_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511547.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(14301..16508)
                     /gene="nrdD"
                     /locus_tag="VQ516_001097"
     CDS             complement(14301..16508)
                     /gene="nrdD"
                     /locus_tag="VQ516_001097"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2511548.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNCRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(16628..18166)
                     /locus_tag="VQ516_001098"
     CDS             complement(16628..18166)
                     /locus_tag="VQ516_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2511549.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(18203..18346)
                     /locus_tag="VQ516_001099"
     CDS             complement(18203..18346)
                     /locus_tag="VQ516_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511550.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(18424..19956)
                     /gene="cls"
                     /locus_tag="VQ516_001100"
     CDS             complement(18424..19956)
                     /gene="cls"
                     /locus_tag="VQ516_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2511551.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(20102..20659)
                     /locus_tag="VQ516_001101"
     CDS             complement(20102..20659)
                     /locus_tag="VQ516_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2511552.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(20656..21906)
                     /locus_tag="VQ516_001102"
     CDS             complement(20656..21906)
                     /locus_tag="VQ516_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2511553.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFEMLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNFSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(22114..22419)
                     /locus_tag="VQ516_001103"
     CDS             complement(22114..22419)
                     /locus_tag="VQ516_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2511554.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(22435..22854)
                     /gene="ruvX"
                     /locus_tag="VQ516_001104"
     CDS             complement(22435..22854)
                     /gene="ruvX"
                     /locus_tag="VQ516_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2511555.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPLDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(22858..23124)
                     /locus_tag="VQ516_001105"
     CDS             complement(22858..23124)
                     /locus_tag="VQ516_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2511556.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(23211..23780)
                     /locus_tag="VQ516_001106"
     CDS             complement(23211..23780)
                     /locus_tag="VQ516_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2511557.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(23846..24244)
                     /gene="spx"
                     /locus_tag="VQ516_001107"
     CDS             complement(23846..24244)
                     /gene="spx"
                     /locus_tag="VQ516_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2511558.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT         6740 a         5398 c         4560 g         7629 t
ORIGIN      
        1 tttttcaata tctgagaaga catcattgat atcttgatta ccttcgatgt catgaaccaa
       61 acctttggca cggtagtgag caatgattgg ttctccttga gcaatattaa catccaaacg
      121 acgttttact gtctcaggct tatcatcttc acgttggtag taatcttctt ctttatagtc
      181 aactggtggg ttaaagacct tgtggaaagt ttctccagtt acgcggtgga tgatacggcc
      241 actcaaacgt tccaagaggc tgtcagggtt cacttcaata ttgataatac cttctagttc
      301 aatgccaagt tcagccaatg ttttgtccaa ggcatgagct tgttcaattg tacgtgggta
      361 accatccaat aagaatcctg tttctttaat atcatcttgt gaaaggcgtt cttttacgat
      421 tccatttgta acttcgtcag gaaccaattc acccttgtca atatatgact tagcaagaac
      481 acccatttca gtttgatttg ccattgcagc gcggaacata tcacctgttg agatatgtgc
      541 aacatggaat tgttctacga tttttgctgc ttgagttccc ttacctgcac caggtaagcc
      601 cataatcaaa agattcatga tttgatctcc ttattttatt tttaaataga agcaaaaagt
      661 cttttcaccc ctatttaaaa acaaaataga agaggggaga tccaactctt acaaatgtca
      721 gagtttaaac ctccactccc tcagtattta ctgattcagt aaatactttt attctgttct
      781 gtccatgaaa ccaacatact tacgtttcaa taggtaacct tccaattgct tgattccttc
      841 gatacctgta gagataatga tcaagagact tgttccacca aaggcaacaa catcagaaag
      901 accaaataca tctttagctg caatcggtaa aatggaaatc acaccaagga agagggaacc
      961 aacagttgca agacgacgaa gaagtttaga catatattct tctgtacctt taccaggacg
     1021 aactccatgg atataggcac cactcttttg taggctctct gctgcttttt caggattaat
     1081 ctgtacaaac gtatagaaga atgtaaagag aataatcaac aaagcataca tggcaatacc
     1141 agttggagaa gtagttgcca acatctcttg tgctaccctt acccaagccc aatcatgacc
     1201 tgtggcactc aaaaactgaa gaatagccgc aggcgctgca gtaatcgaac tggcaaagat
     1261 aacagggata actccagcag ggtttacctt taacggaagg taagagctag atggagcacc
     1321 ttgtgcaacc ttagtatatt ggattggaat tttgtattct gcttgttgaa cataagttgt
     1381 aaagtaaata atcaacaata cagtaataat caaaatgatt acgaaaatga tagatgaagt
     1441 gatacggcta cttgggacgt tcacaaagta gtccacatag atgccctgaa tcatctctgg
     1501 aattgaggaa acaatcccgg caaagataat catggaaaca ccgtttccgt atcccttatc
     1561 tgtaatttgc tcacccaacc aagtgacaat catactacca gctgttaaga tgataccaat
     1621 cgtcagaaaa acttgtggag ttaaagcagt tttaatcaat tgagctccag ccaaggtatt
     1681 aaaaccagct gtaatcccga tagattgcac aaaagcgaga actagagcaa tataacgagt
     1741 agcttgattc aattttcttc gacctacttc cccttgttta ccccactcta caaacttggg
     1801 taaaatatcc atttgcaaga gttggacaac aatagaagcg gtgatatagg gactaactcc
     1861 tagggcaaaa atcgaaaagt tttttagggc attccccgac accaagctca acatgtttaa
     1921 gaaggataat ccacttaaag cattcaagct attggcattc acaccaggaa ctgtaatgct
     1981 agttccgata cgaaagacca aaacgataaa aattgtaaat aaaatttttg atcgaacctg
     2041 cttgacttta agagcttctc ttaataattt aaaaaacata ggtcacctct cttagatgac
     2101 ttctactgaa ccacctttag cagtgatagc ttcttcagct gatttagaga atttagctgc
     2161 tttcacagtc aatttcttag tcaactcacc gttaccaaga attttaatac ctgacttttc
     2221 agctttaaca attcctgctt cgataagaac aactggagtt acttcagcac catcttcaaa
     2281 gacgttcaat tggtcaaggt tcacaattgc gtattcttta gcgttgatgt tagtgaatcc
     2341 acgttttgga agacgacgga acaatggagt ttgtccacct tcaaaaccaa ggcgaactcc
     2401 gccaccgcta cgagcttttt gacctttttg accacgacca gatgttttac cgttacctga
     2461 tgaagtacca cgaccaacgc ggttacgtac tttacgagaa ccttctgcag gtttcaattc
     2521 atgaagtttc atttttattt tctcctcttt tgtaaaatgc tagcgccgat aagggagaaa
     2581 aggttgtctc ccccatcaac tcgcctatac gatgtcatca tagatgacta tatctagttt
     2641 taggggatgg tacagtgcac atcccctaaa acttcattag tttacttctt caactgttac
     2701 taagtgagat actgctgtaa tcataccacg gatagcagcg ttatcttctt taataacaga
     2761 gctgttcaat ttgccaagtc caagtgctac aacagtttta cgttgtgatg gaatgcgtcc
     2821 gattggagac ttagtcaaag taattttaat ttgagccatt ttatcccctt tcttatgcca
     2881 aatcagaaac tgaaatacca cgaagggcag caatttcttc agcgcgtttc aattgtttca
     2941 aaccttcaac agttgcacga acaatgttga ttggagtgtt agaaccaagt gatttagatg
     3001 taatatctgc cacacctgcc aattccacaa cggcacgaac tgcaccacca gcggcaactc
     3061 cagaaccttc tacagcaggt ttcaacaata ctttagctcc accgaattct gaaagaactt
     3121 cgtgtgggat tgttgttcca accataggaa cttcgatcaa gtttttctta gcatcatcta
     3181 ctgctttacg gattgcttct ggaacttctt gagctttacc agtaccaaat cctacgcgac
     3241 cattgtggtc accaacaaca acaagagctg cgaaacgaag acgacgtcca cctttaacaa
     3301 cttttgtaac acggttgaca gcaactacgc gttcttctaa ttcaactgca ttgtctttaa
     3361 atgccatttt ctagtgtcct cctattagaa tttcaatccg ttttcacgag ctgcatcagc
     3421 caaagctttc acacgtccgt gatatagata tccaccgcgg tcgaacacca cttctgaaat
     3481 acctttagcg tttgcacgtt ctgcaacgag tttaccgaca gcaacggctt gttcagtttt
     3541 agttcctttt gaaacttctt tatcaagagt tgaagcactt gcgagcgtta cacccgctac
     3601 gtcatcaatc acttgagcgt agatgcctgt attagaacgg aatacgttca aacgtgggcg
     3661 atcagcagtt ccagagagtt ttccgcgaac gcgacggtgg cgtttttggc ggagtttgtt
     3721 tttatctggt tttgaaatca cagttttcac ctctttagtt ttaaatcgtg tgctatgcac
     3781 aaagttggaa aataggttgg tggttgatga tcaaccactc aacattattt acctgtttta
     3841 ccttctttac ggcgaacgaa ttcaccaacg taacggatac ctttaccttt atatggttct
     3901 ggtgaacgaa ggctacgtac gtaagcagct gtttgaccaa ctacttcttt tgaaattccg
     3961 ctaacaacga ttgttgttgg gtttggaagt tcaaaagtaa ttccttctgg agcttcaact
     4021 tcgtctggat gagatttacc aacagccaaa acaagtttag atccttgaag ctgtgcacgg
     4081 taaccaaccc cacgcatttc aagttctttc ttgaatcctt ctgatacacc aacaaccatg
     4141 ttgttcaaaa gggcacgagt agttccgtgg atagttttca tttcttttga atcgtttgga
     4201 cggtgaagag ttatttcagt accttccaca cggatttcaa tatcttttga gaactcacga
     4261 gtaagttctc ctttaggtcc ttttacagtt acaacgttgt cattgttagc gagttcaaca
     4321 ccagcaggca acacgataac tttattacca atacgtgaca tgtttatttt ctcctgttaa
     4381 attgtcaggc cagaacggcc agttttcacg gggttcagat acttatttag ttcaagagct
     4441 gaactgaaca cgctctaaga actgtgcaaa atagataagt tggcttgttt gtaaacaaac
     4501 ttctccaact tcctaatttt gctttgttct ttacgagctt tgtatcttga ttttaccaaa
     4561 cgtaagcgat aacctcacca ccaacattct tttggcgtgc ttctttatca gtaagcaaac
     4621 cttcagaagt tgaaaggatg gcaattccaa gtccgttaag aacttttgga aggtcttcac
     4681 gttttttgta gacacgaagt cctggtttag aaacacgttt caagttagtg ataactttct
     4741 caccatttgg tccgtattta agaaatacac ggatgacgcc ttgtttgtca tcttcaatga
     4801 tttcaacgtt ttttacaaaa ccttcgcgtt taaggatttc agcaatccct tttttgatgt
     4861 ttgatgcagg tacttcaagt acttcgtgtt tagcttggtt agcattacga atacgagtta
     4921 ggaagtctgc gattgggtca gtcataacca ttttgttttt ctcctcttac tagtagtttg
     4981 caagttgcac ttgctagtta atacatgata caaagagcgt aacagcacga gcaaaaatag
     5041 acaatttaat gcagaagcaa tgctccaaag caaattggtc ttttttgcca aagctgtagc
     5101 tcgtgttcaa attggcaagc ccaattgaac ccgggctaaa ctctgtgtga aaaagataaa
     5161 ctttcctaga aactttagtt tcttcgtcaa gtttcctatt tttacttgga gttttgacgc
     5221 tcttgatatc ttaaattacc aagatgcttt tgttacacca ggaatttgac ctttatgcgc
     5281 aagttcgcga aaagcgatac gactcagacc aaatttgcgg taaactgaat gtgggcgccc
     5341 cgtaactcta caacgattat gtaaacgagt cggtgaggcg ttgcgaggta atttagataa
     5401 accttcgtag tcccctgccg cctttaatgc agcacgtttt tcagcataac ggtcaacaat
     5461 tttttggcgt ttagcctctc tagctaccat tgatttttta gccattagat ttacctccta
     5521 tattattttg caaaaggcat tccaaggcct gtaagcaatg cacgtgactc ttcgtcagtg
     5581 ttagcagttg ttacgataac gatgtcaaga ccacgagttt tgtcaacgtc atcgaagttg
     5641 atttctggga agattaattg ttctttcaca ccaagtgtgt agttcccgcg tccatcaaat
     5701 gattttgttg gaacaccgtg gaagtcacgt acacgtggaa gtgaaactga taccaattta
     5761 tccaagaatt cgtacatacg ttcaccacga agggtaactt ttgcaccgat cgcaacacct
     5821 tcacgaagac ggaagccggc gattgatttt ttagctttag tgataagtgg tttttgacct
     5881 gagataagtg ccaattcttc agcagctttt tcaaggcttt tagcgtttga tacagcttca
     5941 ccaacaccca tgttcaaaac aatcttatct actttaggca cagccatcac tgatgagtag
     6001 ttgaattgtt ctgtcaaagc aggaactact tcattaagat atttttcttt taaacgattt
     6061 gccattatac ttctcctttc cttcgtgatt aatcaagcac ttcgcctgat tttttgttgt
     6121 agcgaacttt tttaccgtct acaaatttgt aaccaacacg accagctaca ccatttttgt
     6181 ccaaaacttg aacgtttgat acgtggatag ctgcttcttt ctcgatgata ccaccttgag
     6241 gaagctcgtt agttggacgt tggtgtttct taacaatgtt aacaccttca acgataactt
     6301 tgtttacttt tggaagggca gtaaggacaa cagcttctgt tcccttatct ttaccagcga
     6361 ttacgcgaac tttgtcgcct ttttttacaa acattaggtt tctccttgat ttttcttacg
     6421 cccataaggg caccctagct tgaagctagg ggactagttt gttcctaaaa attaaagtac
     6481 ttctggagca agtgacacga tcttcatgaa gccaccttca cgcaattcac gtgcaactgg
     6541 gccaaagata cgtgttccgc gaggagtttt gtcttcacgg atgataactg ctgcgttttc
     6601 gtcaaatttg atgtatgaac catcagcacg acgagcacct gatttagtac gaacgataac
     6661 tgctttaaca acgtcacctt ttttaaccgc accaccagga gtagcttgtt ttacagatgc
     6721 cacgataaca tcaccgatgt ttgcaaattt acgtcctgaa ccaccaagaa ctttgatagt
     6781 caagatttcg cgagcaccgc tgttgtctgc gactttcaaa cgagtttctg tttgaatcat
     6841 ttcagttttc tcctttcagg tttgattaga tgatgaccgc ttcttcaaca acttctacaa
     6901 gacggaaacg ttttgtagct gaaagcgggc gagtttccat gatacgtacg atatcgcctt
     6961 ctttggcaac attgttttca tcatgagctt tgtatttttt agagtagtta atacgtttac
     7021 catagactgg gtggttacgt tttgtttcaa ctacaactgt gattgtcttg tccattttgt
     7081 cagatacaac acgtccaaca agaactttac gattattgcg ttccattgaa atttctcctt
     7141 ccctagtcta ttatttcgct tcagattgaa ctgttttgat acgagcgatt tgttttttaa
     7201 cttctttcaa gcgagctgtt tgttccaatt gaccagtagc agcttggaaa cgaagttcaa
     7261 acaattcttt tttcaattcg ttttcgcgct tcgcgagttc ttcttgagaa agaccacgaa
     7321 gttctttaac aaattctttt acttcattaa gtttcatgcc ttctccttat tctgcttcac
     7381 gttttacgaa tttacattta actggcaatt tgtggctagc aagtcgaagc gcttcacgtg
     7441 caatctcttc agatacacca gcgatttcga acatcacttt accacgttta actggtgcta
     7501 cccaaccttc aggtgcccct ttaccagatc ccatacgcac accgatagct ttagcagtgt
     7561 atgatttgtg tgggaagatt ttaatccaaa ctttaccacc acgtttcatg taacgagtca
     7621 tggcgatacg agcagcttcg atttggcggt tagtgatcca gtggctagtt gtagcttgaa
     7681 gaccgtattc accgaatgct acttcttttc caccttttgc ttcaccgcgc atttttccac
     7741 ggaactcacg acggtgttta acacgtttag gtactaacat tggttattta cctcctttag
     7801 tgtttttacg agctggaaga acttcaccac ggtagatcca tactttaaca ccaagtttac
     7861 cgtatgtagt atctgcttct tcccaagcgt aatcgatatc tgcacgaagt gtgtgaagcg
     7921 gaacagttcc ttcagagtat ccttcagcac gggcgatatc tgcaccgttc aaacgacctg
     7981 atacttgagt tttgattcct ttagctccag cacgcattgc acgttggatt gcttgttttt
     8041 gtgcacgacg gaaagcaaca cgttgctcca attgacgagc aattccttca cctacaaggt
     8101 gagcatccaa atcaggttgt ttgatttcga tgatgttgat gtgtacttgt tttccagtca
     8161 atttgttaag ttttgcacgg agtgcatcaa cgttagcacc acctttaccg ataaccatac
     8221 ctggtttagc agtgtgaagt gaaacgttaa ctttgtttac tgcgcgttcg atttcaatag
     8281 ttgaaactgc tgcgtcagca agttcttttt gaacgaattt acggattgca agatcttcat
     8341 gaaggtaatc cgcgtattct ttttcagcat accatttggc atcccaatca cggatgatgc
     8401 cgacacgcat accaattgga tgtacttttt gacccacgat tttacctcct tatttttctg
     8461 caacagctac agtgatgtga gctgtacgtt tgttgattgg tgaagctgaa cctttcgcac
     8521 gtggacggaa acgtttcata gttggtcctt cgtttgcgaa tgcttcagat actaccaaat
     8581 tagctttatc caaaccaaag ttgttttcag cgttagctac agctgagttc aaaactttca
     8641 agatgatttc agcagctttg tttggagtga atgtcaagat tgcgattgca tcggctacgc
     8701 ttttaccacg gatgttatca agaacaagac gtgatttacg aggtgaaaca cgtactgtac
     8761 gagccattgc tttagctgaa gtaatttctg ccatttatgt tctccttatt ttctacgtgt
     8821 tttcttgtcg tctgcagcgt gacctttgta agtacgagtt ggtgcaaatt caccaagttt
     8881 gtggcctacc atgtcttctt ggatgtaaac aggtacgtgt ttacgtccgt cataaactgc
     8941 aatagtgtaa ccaatgaaac ttgggaagat cgttgaacga cgtgaccaag ttttaataac
     9001 ttttttcttt tcgtcgttag cttgagcttc aacttttttc atcaaatgct catcgacgaa
     9061 aggtcctttt ttaagactgc gtcccatttt tatattttct cctttaaatg ttgtaccaca
     9121 gcggcttgca ctcctatgga gcgctaccga gctggcggat ttactagttg cttaagcgac
     9181 tagtttaata ttatttctcg ttgcgacgac gaacgataag tttgtcagat ttcgctttct
     9241 tgttacgagt tttaagacca agagcaggtt tgccccatgg agtagatggt gctttacgac
     9301 caactggtgc tttaccttca ccaccaccgt gtgggtgatc gttagggttc attacagaac
     9361 cacgaactgt tgggcggata cctttccaac ggctacgtcc tgctttacca aggtttacaa
     9421 gtccatgttg ttcgtttccg acaacaccaa ctgtagcacg gcaagttcca agaatcatac
     9481 gaacttcacc tgattgaaga cgaacaagaa catatttacc ttcagaaccc aatacttgag
     9541 cagatgcacc agcagcacgt accaattcac caccacgacc tggtttcaac tcgatgttgt
     9601 gaatcaaagt accaactggg atgttagcaa gtggaagagc gtttccgact ttgatatctg
     9661 cttctggacc tgaaacgata cgttgaccta cttcaagacc ttttggagcg atgatgtatg
     9721 ctttcacacc gtcagtgtag tgtacaagag cgatgtttgc agaacggttt ggatcgtact
     9781 cgattgtttt aacaactgct tcaacgttgt ctttattacg tttgaagtca accaaacggt
     9841 aaaaacgttt gtgtccacca ccttggtgac gaactgtgat acgaccgttg ttgttacgac
     9901 cagccttgct cttcaatgca acaagcaatg atttttcagg agtgcttgtt gtgatttcag
     9961 cgaaatccaa agaagtcata ttacggcgac cgtttgttgt tggtttataa acacgaattc
    10021 ccacgatatt tcctccttag attattcagc ttcagcagca aacaactcga ttgctttaga
    10081 atcagctgta agtgtgatga tagctttttt agttttgtta gtaaaaccag tgtaacgtcc
    10141 aacacgttta gcttttggtt ttacgttgat tgtgttaaca ttggcaactt taacaccttc
    10201 gaaagcagct tcaacagctt gcttgatcaa aagtttgtgt gcacgagtgt caacttcaaa
    10261 tacatatttt cctgcttcaa gttgagccat tgagctttca gtgatgacag gttttttgat
    10321 aacatcatac aaattcatta tgcaagaacc tcctcgattt tagagatagc tgcttgtgtg
    10381 acaagaagtt tgtcgctatt tgcgatgtca agaacacttg cagttgtagc agttgcaact
    10441 ttcacgtttg gaaggttacg agctgaaaga gctgcgaatt catttccttc ttcaaggata
    10501 acaagaactt tagaatcgat gctcaatgct gcaagaactt ttgcaaattc agcagttttt
    10561 ggagctgtaa atgaaagagc gtctacagct acgaatttgt tttcagcaac tttttcagag
    10621 taaactgatt taagagctag gcgacgaact ttttgtggaa gtttgtagcc gtatgaacgt
    10681 ggagttggtc cgaagacaac accaccacca cgccattgtg gtgagcggat agaaccttga
    10741 cgagcacgtc cagttccttt ttgacgccat ggtttgcgtc caccacctga tactgcagag
    10801 cggtttttaa cagcgtgtgt tccttgacga aggcttgcgc gttggctgat gattacatca
    10861 aacacaactg attcatttgg ttcgatacca aatactgcat cgctaagaac aacttggcca
    10921 gcttctttac cagtttggtc aaataatgtt acgtttgcca ttgtgactga tttccccttt
    10981 ctttattatt taccagcttt aactgctgat ttgatagtga taagagattt cttagcacct
    11041 ggtacgttac ctttgataag gataacgttc ttttctggaa caacttgtac aacttcaagg
    11101 ttttgaattg ttacgcggtc gccacccata cgccctgcaa ggtttttacc tttgaatacg
    11161 cggttaggtg caacaggtcc catagaacct ggacgacggt ggtaacgaga accgtgagcc
    11221 attggtccac gtgattgtcc gtggcgtttg ataacacctt ggaaaccttt acctttagaa
    11281 gtaccagtta cgtcaacaac gtctccagct gcgaatgttt caactgtaat ttcagcacca
    11341 acttccaagc cttcaacgtt tttgaattca cgaatgaagc gcttaggagc cgtgttagct
    11401 ttcgctacat gtcctttagc aggtttgttg ctcaatactt cgcgtttgtc atcgaaacca
    11461 acttggatag cgttgtatcc gtctgtttca acagttttaa cttgaagaac aacgtttgga
    11521 gttgcttcaa taactgttac agggatcaat tcgccagctt cagtgaagat ttgagtcatt
    11581 cccacttttt tccctaagat tccttttgtc atgagaaaat agttcctttt ctatattttt
    11641 attcaaaaag tttttaacga gcgtttttta tgctcaagtc taagatacaa agggcgtcaa
    11701 actcaacgtg aaaataggaa actgacacag tgtctagcag acgcgaggaa gtttatcttt
    11761 ttcacaccga gtttagcccg tgttcaatta gattgaaaca ccagcctctg ctcttttata
    11821 ttttagatta aagtttgatt tctacgttta caccacttgg aagatccaat ttcatcaagg
    11881 catcaactgt tttttgagtt gggttaacga tatcgatcaa acgtttgtgt gtacgcattt
    11941 caaattgttc gcgagagtct ttgtatttgt gagtcgcacg aatgattgtg tagaggctac
    12001 gttcagttgg aagtgggatt ggacccgcaa cttgtgcacc tgtacgagta gctgattcta
    12061 cgatttttgc agccgctgtg tcaagcgtac ggtgttcgta agctttcaaa cggatacgga
    12121 tttttttgtt tgccatcttt ttctcctttt cgtctattta agataatagg ctagctccac
    12181 aagaaaaccg acgcggtgtt gcgtggcaat gcaaccgagc gtgtcgcaac ctcttgcatc
    12241 aaaactaagg ctgtaattta cagcaccata atagaataac acaaatcccc tgcgattgca
    12301 agggatttgt taggtttttt cgttttttgg ggatgaaact gttttcctac tttttctttc
    12361 taaaattagg gattttcttc tatatttctt ctatacataa gttaagtcct cttgacatca
    12421 aatttatctt ctgattggtc cactaagata tccgccttag actcagtttc tttatagtat
    12481 cgcagatact gctcccgtct catctgatgg ctagccaata taaaggatgc atcgcgattt
    12541 ctcacagtcg tatctctagc aaggcgtcgc tttaattcgg tctcctcatc cgtgtagaaa
    12601 cagatggttt tttcaaagag ttccttgggt agaaagccaa cagacatccc ttcgacaatc
    12661 aaaattggtt tggctccaga caagacctca ctagccttcc aaggttcttc aattgtcaag
    12721 acatccatac ccgcctgcaa ggcaaggata tctctctgca aactctccag ttcatgcgcc
    12781 actggcagac tggctgtcac cttttgattc ggcgcgtcct tgggtactac caaatagcgt
    12841 ccggaggtga tatagggatc tgtttctagc aaatttactg tagtagaatc taaagcttgg
    12901 tacaattcct gtgcaaaggt tgatttacct gaagctccat gaccgtatat tcccagtgtc
    12961 ctgactttcc ccgtctcgat ctctgagact agttggtcta ctaagtcttt tttcttcatt
    13021 ctctcttcac ctgttcatag atttcttttc cgtcattgag cttgtcccaa atcactactt
    13081 gcccactttt gagcgatttt tgcacatcga taattcgttg gttagacgaa cctcgaaact
    13141 ggagcataag atttctctta gttcgatcat atcttccatc gacaagaatg tcaatcagtg
    13201 acaagagttc cagtttatct ggagtttcca acatcatttc ttcccaagtg tagcccgtcc
    13261 aggaccagat gtccttgtct ggcaattcct tccgaatccg cttaacaagt ggcaagagaa
    13321 tcccagtatt aagaaaaggc tcccctccta gtaaggttaa cccctgaaca taaggctggg
    13381 caagatctgc cataatctgt tcttccaact cagctgtata gggaatgcct gcattgaagg
    13441 accaggtcgc tacattataa cacccctcgc agtgaaacat acagcctgct acatagagag
    13501 agttacgcac gccttcgcca tcaacaaagt taaaggcctt gtagtcaatg atacgacctt
    13561 gactaagttc ctcgcttttc cattcttgtg gttttggatt attcattcgc tacctctatc
    13621 caataacgct cgactccatt gcgagcatcc tcaaatattc caccatttgc tagaatgact
    13681 gctctgctag caggattatt cacgctacag gtcaccagag ctttcttgat gttcttttcc
    13741 ttagcaactt gcaagccctg acggagagtc tcttttgcat aacccttgcc tctttcagat
    13801 ggacggatgg agtagccaat gtggccagca taattcagta acccctcatt cagtctcaat
    13861 cgcagattca aaaatcctag agcacaacct gattcaccaa acaatacgaa ctgtatcgat
    13921 ggaacgcgat tttcaggtag ccatattccc atttctttat tacggttacc ttctagccac
    13981 tcctcgtaag aaaaatcctc aacatcccaa aatcctccat catgggctga ttggctcttt
    14041 tcaaactctg tcatcatatc taaaacagct ttcttatccg ctaatcttgg tctgcgtaat
    14101 tccatcctta cctcccacta tgagaaaagc gagagctggc tctcactttt attttttctt
    14161 gttcttgttt aaaaattgtt ctctgaagct aagcaagcgt tctttcttac cagctccacc
    14221 tttagagtgt ttctcgtgat aacgggtcac ttgtgcgcgc cccttatcgt ctagttgata
    14281 ttttcccatt tcatttcttt ctaatttgtt acttgatgcc cagctatttt aatcgttgaa
    14341 ccattcatat gtttgacacg cgcagcgatt tccttgtgac gcccgttgac catcggtctt
    14401 gcttgaggat tacctaggta gccacaagtt cgcttcacca catctactgt tttagggtcg
    14461 ctattgccac agtttggaca agcaaaccct ctctcagttg gttcaaaatc cccttcaaag
    14521 tcacacttgt agcaacggtc aatcggagta ttggtgccta gatagcctac acggtcataa
    14581 gcataatccc agacagcttc caaggccttt ggattttgct gaaggactgg atactcacaa
    14641 taatggatga aaccacctga cgcacctgct tctggataga ctttctcaaa gtccaatttt
    14701 tcaaacggtg ttggattttt acgaacatcg tagtggaaag agttggtgta gtattccttg
    14761 tctgtgatat caggaataga gccaaacttg tctatatcta gtcggcagaa acggtctgtc
    14821 agactttcgg atggtgttga gtagatagag aaatggtagc catattggtc tgaccactct
    14881 tctacacggc gtttcatatc gtgaatgatg tctagcgtga attccttagc atctggatta
    14941 ctttcccagc tgttaccaaa gaaaactgtc gctacttcat acaagccgat atagcccagc
    15001 gaaacggtcg cacgacaatt cttaaagagc tggtcaacac tttcttcttt acctagacga
    15061 tggccaaaag caccgtactg ataaaggata ggagcattcg ctggtgtcgc ctctttagtg
    15121 cgttcgacac ggtaaacaag agcatcttct gcgatattca ttcgctcgtt gaagatttcc
    15181 cagaacttat tcatatcacc ttcagactca agagcaatac gaggcagatt aaccgtcaca
    15241 acacccagat tcatgcgacc tgaattgact tctacaccat tttcatcctt ccatccttgg
    15301 aggaaagaac ggcaacccat aggcaccttg aaggatcctg tcaagtcaac aatcttatcg
    15361 taagataaaa catctggata catccgtttg gttgcacact caagagccaa ctgcttgatg
    15421 tcgtagttgg gagttccttc ctctaaatta aggcctcttt tcagagtgaa aataagttta
    15481 ggaaagatag ccgtacggtg ttctgaacca agacccttga tgcgaatgtt taaaatagct
    15541 ttttgaattt ctcgttcaaa acgactggtt cccagaccaa aacctagcga agtaaaaggt
    15601 gtttgtccat ttgaagtgaa gagagtattg atttcatact caagagattg catggcatcg
    15661 tagatgtcct tttgcgtttt cttccaagcg taatcttccc gtttttcagg caatacccac
    15721 tcttctgcat ctttgagatg tttttgatag ttcttctctg cataaggcgc caaaatttca
    15781 tcgatacggt cagctgaaca gccaccgtac tggctagaag caacgttggc aatgatttga
    15841 gaaatctgtg ctgtcgcagt ctggatagac ttgggactct ctacctctgc atttccaatc
    15901 ttaaaaccat tttccaacat acccttaaaa tcaatcaaac agcagttggt cataggggta
    15961 tagggactgt agtccaaatc gtgatagtgg atatccccct tttggtgggc attggctacg
    16021 tgcttaggaa gcatttgcag tccgattgat ttcccaacaa tccctgctgt caaatcacgc
    16081 tgagtgttaa agacatcact gtctttatta gcattttcat tgacaactgt ctggtctttg
    16141 ttgagaagtt tatgaatact aaagttgata tccgtcgctt ttgagcgctc aaaatccctc
    16201 tgtgtccgat aagtaatata ctcctcagcc agcgcatatt ctttggcttc aaggagttca
    16261 tgttctacga tattttgaat ttcgtaaatc ttaattccct gtggaaagcg actatgaatt
    16321 tctgtaataa ttctctcagt cagatcattg aggcgttttt caaccagggg tgtcacatcc
    16381 ataaccttgt cagctgcctt gtggagagcc ttgtcaatct tgtccacatc aaatacaaca
    16441 cgtctcccat ctcgtttctc gacgaagaga gttggcaaaa ttgtaatttt ttcttctagt
    16501 gcaatcatat gcatgctctt ttctaaaatg ttctttctaa aaagtattat accactctaa
    16561 aaagaaaaat caatatcttg tgttaaaaat cctaaaattt ttaaaaatac acaagatatt
    16621 gatttggtta tttaatttta tagactttaa actctgagta atcagtcttt acttgttggt
    16681 aattttcttt taaaagtgtt tccacttcag accagactac caccttgtca tttaccacaa
    16741 tcaccttagg ttgtttttct ttcaagtcat taagtaactt attacgattt tcctcagttg
    16801 ctgtatagtg caacggggac aaaatcgccg atggcgacaa gcgctcactc ttacgataaa
    16861 gagtcgcagt atcatcccat gcatagatag catcttccgt atttgtctcc tcttttacca
    16921 aatcagcaag acgattacgt tcttgataag gtactggata aaggacaaac ttcatcaaaa
    16981 aaggaacaga caaaagatag acaaacccaa aaactagcag atatagattt cctttgaaga
    17041 aacgactcca taggctaact ggcgtttctc ttcggcttct tctagcacta cgccccctct
    17101 gttgactctt gatattggtt accaataaaa gtaggaaaac agggaagacg accatcaaat
    17161 aagaagcgtg cagaggctct tgtgaaaaga aaagtaaaat caaacctaaa attaccacaa
    17221 gacttgcagg aaccgaaatg acgtatagtt taaagggttt ggattgaaag agacctaaaa
    17281 agatacaaac cacaaatccc aaaccaaggg acaacaagcc ataaaaaaac atattctcta
    17341 aaattttaga agtaaaatca aagcgaatac tgtcaatagg ataacgaata ccactaatcg
    17401 catccccaaa acttcctgtt gcagcactat agtaggcagt tggataaaag acaagtgaaa
    17461 aacctaaagc cactgcaaga aactgataaa acccatgcgc aaagcgtcta tgcccaaggt
    17521 taaagaccaa caagcctaaa ctcactacag caataaacag gagtgatgat aagggagcaa
    17581 agaaaaatcc gcctgccaaa gctaacccaa tccgtacaaa tcccttatca tggcttggat
    17641 tgcttaggta agccgcaact aaactaaagg ctgcgaataa gaaaggaagc gctaaaagag
    17701 tcgcatagcc tccaccaaaa gcaagacctg taactagcat gtaaaaaata gtcaccagat
    17761 gtccagcttg gtctccttgc tctgtcaagg tgtccgctga tctaaaaagg aaaaatcctc
    17821 ctgctaccaa ggctaaccac tcaaagatgg caaagaaaaa tccgccctga ctcacgtaag
    17881 tcagcaaata ataaagcaaa ccttgacttc cataatagtc gctgtaaatc ttccctgtct
    17941 gatgaagcgc ccaacctgca taaaaatcct gcacttcttg tgcactcatt aagtcgagta
    18001 atagcggtac tcctagagtt atccccgtta caagcgtact ccatagtaaa attttcacca
    18061 aaggaagacg acttgattca cgatgatgcg attcttgctc gatttggtat tctagaggtt
    18121 cacgattctc cttatgaact tcttctactc taccatacac actcatatcg tttctcctgt
    18181 tcaatttatc tgtcttagta tatcaaaaag tcctaggatt gtcagcttgc gatggctgtt
    18241 tgagtgattt tttgcatagt ttcacaaagg tttcaatttc ccgaaagcgg tgtaaatgtg
    18301 tctgtttgaa ctcaatcata tcattcactt cttccttagt tagcatgaat tcccctcctc
    18361 tttctttcta ttcgtaaaag acaagaaaaa taggtcgggt ttgtatccta agttctagat
    18421 ttcttataag ataggggcga atagctgggc gatttctttt acaagctttt gataccagct
    18481 cgttttgata gaatgaggat agacttcctg agaatctgca aatatatttc gaaaatctcg
    18541 ggcgatttcc cttatagaag gagttttata gagtaagact gcattttcat aatggtgtac
    18601 caagctccgg tagtcgagat tgattgtccc caccaccgca aaatcttcgt ctaccaacat
    18661 ctgcttacta tgaataaaac ctggactata ctcataaatc cgaacaccag cagaaagaag
    18721 gtcgggataa gctcctctcg tgactaactg aatgaacttc ttatctggta tgtaaggggt
    18781 gataattcga acatcgaccc ctctcatagc tgcatttttg attgtctctg ttaaatcata
    18841 atctataatc aaataaggtg tcgtaatata gaccgattcc gttgcttgat tgattaaact
    18901 ctgataaact tttttcccta cctgcgctcg aaaaattggc ttgggtccac ttccgtatgg
    18961 aatggttaaa ccgtcactcg ggatagaatg attttctaaa tgatattgat caaaatcact
    19021 aatttctcct cgattaatgt accaagtggt caaaaataag cgtgtcaggg cttttactgc
    19081 tagtccgtct aagcgaattc cactatcctt ccaataacca aatctctcga cgtggttaat
    19141 gtactcatct gccagattga ccccaccagt ataggctatc tgaccatcaa caatcaatat
    19201 ttttctatga tctctgttat tataagccac tgtcaaacga ggaataactt tattgaattt
    19261 atgggcctcg atgcccagct gacgaagtcg atgtgcataa tctcctgtta aagtagccat
    19321 acagccgata tcatcataga gcatcttaac ctctacaccc tgagctacct tttgctctag
    19381 tatatctagt atgcgattcc acatcaaacc ttcttctata atgtaatatt ccaagaaaat
    19441 aaatttctca gcctttttaa gatcttctac catctttttc cacatagctt ctccgttagg
    19501 aaaaaatgta gaggcagttt gatcatagat gtcagcattg gtatccatac tcaataagga
    19561 cttgatgact ccataagctg ccttgtcact ttcttttaat ttctctttta gtagctggct
    19621 atttgcttct tggaaatgca tagagcctag cttcttcagt tgtttgattt cttttttgga
    19681 caatcgcctt tcaccaaaca tcagatagag taagggacca aatactggca caaaggctac
    19741 taacaaccag gttaccttat tctcaggagt cgtattacgg ttgactatcg aaatgattgt
    19801 gatgatactc agtagaatga ggacagtaat ccataaaatg ggagccatgc gtcctaaata
    19861 aagaaagaga ccaaaaacaa gacaaagttc aagtagcata atcgaaagac taaagccata
    19921 cttggacatc aataattgaa atttcctata tttcatatcc cctccttgat agtttgaatg
    19981 ctaaaaacaa gtaattgata ctagtataac gcaggtattc ggtagaaggc aagtatttat
    20041 gattattttc taggtaaaag taaaaaggct gaaaaatcca gccctttcta cttgcatagt
    20101 cttatttagt taaaacgatt tggtccgatg ggtctctatc catatcatct acgacgattt
    20161 gattaccgtt tacagtgtaa attttgacat catcgccaat aatcatgcgt tgatttgctg
    20221 aatcaaatgt aacctgtttg actttctgat ccccgtcaga ttcgagctca gtccatgtac
    20281 cagtcgttcc agttaccact aaagtgatac ggtctccgtc atcctgacca gtataagtcc
    20341 catcaatatc ggtcggttga gcaacaggtt ggttttgtac tggagccgct tgattttggt
    20401 tacctcctgc atttgcagta ttggtattct gttgagcagg ttgttgagca ggctgttgga
    20461 cctgctgctg agcaacaggc tgttgaacct gttgagctct ttgtgaacaa gctactaaga
    20521 gactaaaact tgctagagaa gcaagagaaa gtgtgaatct ttttaatttc atgatgaatt
    20581 tcctttctgc taccaattta gagaaatttt ctctaaccag caattcccct agtataacaa
    20641 gttcaaacaa gggggtcaat ttatctgctc acggtccagc aggtagcccc gtacttctga
    20701 gataaaatag agagaccctg taacgaacag caagtcttga gcgtctgccc tttcttcgaa
    20761 actgctgata aattctcggc aagaagaaac tatatcgtaa cctgtcacat ccctttcgtc
    20821 caaagccccc tgatagtcaa agccggtcac cttgagttcc acctgaggca atttttcagt
    20881 caggtaacct aacatccctt gataatcctt ccgtttcaag gctccaaaaa ggatttgagg
    20941 tcgatagcct tcctgctctt tttctttgat aaactcaacc aagcgagtca aggcagggag
    21001 gttatgagca ccatccaagt aaatctgtgg gcgaatacgc tccaagcgac cagcccaatg
    21061 ggtctgttcc aaggcctttc ttacagcctg ttcatcaaca gcttcctttc cttctcccat
    21121 aaaaagaaga aaagtttgca acgccaaggc tgcattctcc tgctgataga ctccttctaa
    21181 gcctatgttc agctgtgaaa aatttagtaa agagcttgaa aaatcaccat tcagcattaa
    21241 aaaatcttga cctgcctgat aaaggttaac agctaaagat tcggcttttt tctgacagac
    21301 aagcctagct tctggaggca atttcgcaat cactgccttc ttaccagcct tgaaaatacc
    21361 agctttctgc tctgcaattg cttctagact atcacccaag gtttcttgat ggtcaagccc
    21421 aatggaggtg atgacagcaa gctctccagt taccacattg gtcgtgtcaa gtaagccacc
    21481 aattcccact tctaataaaa ccaaatccac ctcttgctcc ctaaaataaa gaaaagcaac
    21541 caaggtcagc atttcaaaaa aggacaactg gtcaggagtt tgcagaagcg ttttctccat
    21601 ctccttgacc tgatcagcca aacggataaa gtctgcgtct gctataggtt gcccattaat
    21661 gcagattcgg tcattgatag agacgatatg aggggaggta aaggtcgcaa cttttttgcc
    21721 atgccccata aataattccc tcataaaagc aatggtagat cctttcccat tagtccctgt
    21781 tacgtggata atagggtaag actgctcagg atttcctaac aaatccaccg cttgctgtat
    21841 tcggcccaaa cctgatcgaa aattcaaacc aatccgacta tgaagccatt cttctacttc
    21901 aaacatacac atctccttga caaaagtcca atcaattatc gcattaaagt atggttacta
    21961 ataaaaacaa ggccaggatt ttcgtcccga cctcttacct ggttagctaa taactagcca
    22021 ctatgaatgt gaatatgggc taaaaacatc cactagatgt tccaactctt ccccatttct
    22081 gggagttggg ctgatacagt ctcccagact gtatcactcc tccataaagc tgttgaagac
    22141 ttcttcaatc atgttccatt cgtcttctga gtcttctggg attggttgca attcgccttc
    22201 tgttccatct tcattttcaa tgaatgagta agcttggatt tcaacttgtc cgtcttcgtc
    22261 ttcttctgcg ttaactggta ctagaagaac atagttttta ccaaattctt cttttccatc
    22321 aattgtcaaa aggatttcaa acaaggtttc atttccttgc tcatctacta gtgtgattag
    22381 ttcacgttct tcgtggtcgt ggttatgatc gtgtgacata gcctctcctt tatattaaaa
    22441 ttttctatct aaataatttt gtaaaatcag ctgagctgct aacttatcaa tgactttctt
    22501 gcgcttattg cgactgatat ctgcttgttc aatcaacatg cgctcagcag ccactgttgt
    22561 caagcgttca tcctgatagt ctaatggtaa accaaaaaac tcttctagct ttgctccgta
    22621 ggcttgacta gcttctacgc gcggtccact tgtattgttc atgtttttag gcaagcccac
    22681 tacaaatcgt tccaccttgt aagtatcaac caattcctta acgcggtcaa aaccaaattg
    22741 gccttgttct tcatttatct ggatgatttc aagcccttga gctgtaaaac caagcggatc
    22801 gctaatcgcc acccctaccg tttttgaacc gacgtccaat cccataattc tcataggtta
    22861 tagatcgact ccttgtcctt tgaggtagta gcgaaccaat tcctcaacga tttcatcacg
    22921 ctcatactta cggatttgat ttcgtgcatt attataacga ggaacgtagg cagggtctcc
    22981 actcaatacg taacctacga tttggttaat tgggttgtaa cccttatcgt tcaacgaagc
    23041 ataaacatct gtcaaagttt cgctaatttc ttttttattg gaatcgtcca atttaaaacg
    23101 tactgtttct tcagtaaatc ccattctaac accctctttc cttagaatag taccattata
    23161 gcataattcc ttaccttcta caattcaggc agtctattta tttggatttt ctattgttct
    23221 gtcgcgccat ttgccaatct atctgaaata tatttgcttg gttcattttt caaaagattt
    23281 tccaaaccaa tattcttcag atgttccaac tgggaagcct tcttgacatc cagaacttga
    23341 aaatcaaaac tagtcgttgt ttgaagttcc gttgcgctca atagttttgt ttcaagtttg
    23401 aaacctgcca atttacgagc ttcaatgata gacttatcct tctcctccgc ttcaagaaga
    23461 gctttttgag tttcctccac tccatgttgg tcaatataag tcttcaactc atcagagact
    23521 ggacgttttt gttggatagt tagactcaaa aaagtcttgt ccttgtatgt aatggtttgt
    23581 gtctgctggc taccatcatc tgacttgggc aagaccaaag tcttagttac aactgtattc
    23641 ttctcagcat tttcaataac tggcaatgcc gactgaagcg tatctttttc tgtttttgta
    23701 gctggtccag tttctttttt ctgtccgcaa ccaaccagga caaaaaggaa agctagacta
    23761 acaagaacta tttttttcat ttctttcttc tttctttttg aaattaaaat agaataagac
    23821 tgggaagtgc tcccagcctt gatgtttata gagctgcacg caaacgtgct tctgcatttt
    23881 ctacattacg gacagagcgt ggtaggaagg cacgaatatc gtcttccttg tagccaactt
    23941 gcaggcgttt ttcatctaca aggattgggc tctttaaaat tctcggtgtt tccataatca
    24001 gattgagaac ttcattgaca ctcaaatctt caatatccac tccaagggct ttggcatagc
    24061 gatttttaga cgaaacgatg ctggctattc cgttatctgt tttggttaga atatccagta
    24121 attcttctct cgtaattcct tctttaccaa ggttttgttc tttataactt aactggtggg
    24181 cattgagcca ggtttttgct tttttacagc tagtacaact tgagactgta taaattttaa
    24241 tcatgtacct acccctttcg ctacatgtta ctatcagttt agtctattat accataaaaa
    24301 acatccgact tgcgacctat ttttaat
//
LOCUS       DAVBCV010000027        23964 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBCV010000027 DAVBCV010000000
VERSION     DAVBCV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.027"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(254..2512)
                     /locus_tag="VQ516_001108"
     CDS             complement(254..2512)
                     /locus_tag="VQ516_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2511559.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2741..2971)
                     /locus_tag="VQ516_001109"
     CDS             complement(2741..2971)
                     /locus_tag="VQ516_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2511560.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3161..3844
                     /locus_tag="VQ516_001110"
     CDS             3161..3844
                     /locus_tag="VQ516_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2511561.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3844..4578
                     /locus_tag="VQ516_001111"
     CDS             3844..4578
                     /locus_tag="VQ516_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2511562.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDIAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4732..5589
                     /locus_tag="VQ516_001112"
     CDS             4732..5589
                     /locus_tag="VQ516_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2511563.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGYRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5731..6603
                     /locus_tag="VQ516_001113"
     CDS             5731..6603
                     /locus_tag="VQ516_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2511564.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLMTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6885..7568
                     /gene="rpiA"
                     /locus_tag="VQ516_001114"
     CDS             6885..7568
                     /gene="rpiA"
                     /locus_tag="VQ516_001114"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2511565.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7582..8793
                     /locus_tag="VQ516_001115"
     CDS             7582..8793
                     /locus_tag="VQ516_001115"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2511566.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8795..9337
                     /locus_tag="VQ516_001116"
     CDS             8795..9337
                     /locus_tag="VQ516_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2511567.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9353..10162
                     /locus_tag="VQ516_001117"
     CDS             9353..10162
                     /locus_tag="VQ516_001117"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2511568.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10638..11618
                     /locus_tag="VQ516_001118"
                     /pseudo
     CDS             10638..11618
                     /locus_tag="VQ516_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            11643..12155
                     /locus_tag="VQ516_001119"
     CDS             11643..12155
                     /locus_tag="VQ516_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2511569.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPISAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12802..13512
                     /gene="deoD"
                     /locus_tag="VQ516_001120"
     CDS             12802..13512
                     /gene="deoD"
                     /locus_tag="VQ516_001120"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2511570.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13535..14059)
                     /locus_tag="VQ516_001121"
     CDS             complement(13535..14059)
                     /locus_tag="VQ516_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2511571.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14112..14348)
                     /locus_tag="VQ516_001122"
     CDS             complement(14112..14348)
                     /locus_tag="VQ516_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2511572.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14416..15336)
                     /locus_tag="VQ516_001123"
     CDS             complement(14416..15336)
                     /locus_tag="VQ516_001123"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2511573.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15526..15930
                     /locus_tag="VQ516_001124"
     CDS             15526..15930
                     /locus_tag="VQ516_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511574.1"
                     /translation="MKILKRYILELCFILSFALPFIKGTNADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16146..16727
                     /locus_tag="VQ516_001125"
     CDS             16146..16727
                     /locus_tag="VQ516_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2511575.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16724..18001
                     /locus_tag="VQ516_001126"
     CDS             16724..18001
                     /locus_tag="VQ516_001126"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2511576.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            18019..18681
                     /gene="deoC"
                     /locus_tag="VQ516_001127"
     CDS             18019..18681
                     /gene="deoC"
                     /locus_tag="VQ516_001127"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2511577.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18668..19057
                     /locus_tag="VQ516_001128"
     CDS             18668..19057
                     /locus_tag="VQ516_001128"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2511578.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19147..20199
                     /locus_tag="VQ516_001129"
     CDS             19147..20199
                     /locus_tag="VQ516_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2511579.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20343..21878
                     /locus_tag="VQ516_001130"
     CDS             20343..21878
                     /locus_tag="VQ516_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511580.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            21871..22929
                     /locus_tag="VQ516_001131"
     CDS             21871..22929
                     /locus_tag="VQ516_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2511581.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22932..23888
                     /locus_tag="VQ516_001132"
     CDS             22932..23888
                     /locus_tag="VQ516_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2511582.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6956 a         4329 c         5459 g         7220 t
ORIGIN      
        1 gcaatacctt ttacgaggct cttttgtcct gctcttgttt caattgacta tagcagattg
       61 aaataagatg agaacaaatc gattgggaaa gtaaaattaa tttctataaa tgttttagca
      121 attgtttcgt actatttcag attcagtcta ctatatatca taatggacaa agggcaagct
      181 gtcaagaaat tacagttatt ttagtaaaaa aagaagcaga aaaatttaat tttctactcc
      241 ttttctatag gatttatttt tctttttcag aacttttaac ttcagctttt ttggcagatt
      301 taatctgaat gtttcccttg ctagtcataa ctgctttgag atctttttcg cttggatttt
      361 caaggtagta gtcagtgatt gtgtcctcaa tatagtcttg aatagtccga cgaagtgggc
      421 gtgctcccat ttttggatca taacctaggt caaccaactt ttccttgacc ttgtcagtta
      481 catccaaacg aatgttgttg ctagagaggc gcttgttaac atctgctagc atgagctcga
      541 caatctgaag gaggttatcc ttgctgagag ccttaaattc gataatgcca tcaaaacggt
      601 tcataaactc tgggctaaag aagttaccga gttcaccgag gacagaattg gtacgtcctt
      661 ctctagcagc accaaatcca acgctagctt cggtctttcc tgtacctgca tttgaggtca
      721 tgataatgat ggcatccttg aagctaacgg tgcgtccttg cccgtctgtc aaacgaccat
      781 cgtccaagac ttgaagaaac atgtgcataa catctgggtg agctttttcc acttcatcga
      841 gaaggatgag agaatatgga ttgtggcgaa ctttttcagt taattgacca gcctcatcat
      901 agccaacata acctggagga gcgccgacca acttagccac actatgtttt tccatgtatt
      961 cactcatatc aaagcgaatc atactatcag cagaaccaaa aagttcgata gccagttgtt
     1021 tggaaagttc tgtcttaccg acaccagttg gcccaacgaa gaggaagctt ccgattgggc
     1081 ggttaggggt accaagtccg acacgattac ggcgaatagc cttggcaatc ttatcgactg
     1141 catcatcttg gccaataaca tgagacttga gatcttcggc tagatggatg agttgagatt
     1201 gttctttctc tttcaaatca ccaacaggga tattggtttt ctgctcgata atgtgctcaa
     1261 tagttttctc gctgatgata ggagtatcct ggtctgtgat ctttttcttt tgcatttcct
     1321 tatacttggc aatctggtcg cggaagtagg ccgccttctc aaaatcttct tctcgtgtag
     1381 cttgagactt gagattttca gcctcaatca agcgctgatc aattacttta ggatccacaa
     1441 aattcaaggt caagttcatc ttagaaccag cttcatctag gaggtcaatg gccttgtcag
     1501 gcaagaagcg atcttggatg tagcgattgg aaagagttgc agctgcttca atcgcagcat
     1561 ctgtatattg aacgtggtgg taatcttcgt atttcttttg aatccctttg agaatagtga
     1621 ttgtttcgtc caccgttggt tcatcgactt taacaggctg catacgacgc tcgagggcag
     1681 catccttttc aatgatacgg tattcattga gggtagtagc accgactagt tgcagttctc
     1741 cacgagcaag ggctggcttg aggatatttc ctgcgtccat attaccatca ctcgcagaac
     1801 cagcaccaac aatttcatgg atttcatcga taaagaggat gatgtcttca cgtttgcgaa
     1861 tttcttccat gagtttttgc atgcgttctt caaattgtcc tcgaatcccc gttccttgaa
     1921 ctaagctaac cacatccaga cggatgactt gtttaccttg gagtttatgt ggcacatcgc
     1981 catcgacaat tttctgagct agaccttcga caacggccgt ttttccgaca ccaggttcac
     2041 cgataaggac aggattattc ttggttctac gattgagaat ctcgatgaca cggataatct
     2101 catcgtcgcg cccaataacg gggtcaatgt ctccacgacg ggcaatttca gttacattaa
     2161 taccaaattc ttccagcagg cctttttctt ggctaggtgg cggagtttga gcagatccac
     2221 gattttggga accataaccg ccgtttccac cgtaacctcc acctgattgg gttgggggaa
     2281 taggaggagt attgctagaa ggtctgaaat tgtttagatc attgaagaaa tcaccaaagg
     2341 gatcgaagtc acgattgttc agatccgtca tacctttgaa gaggctattg ttaggatctg
     2401 ttttgataat cttatagcag ttttgacaga ggtcaatttg tttttgtttt ccattgagat
     2461 tggtgtaaag atgaattgtt gagtcgttaa ttttacagtt ttgacaaagc atacatctac
     2521 ctcatttctt tctttagcct gacttgttta aaggtcaaaa atagtcaaat tctatactct
     2581 cattataaca ggattggggt gtaaagcaag taaaaagatt gaagctttga gaagttgtta
     2641 caatgaaaat ttttgtgaat ttggactata aaaaaatcct atttgtgaga caaaaaggat
     2701 tttacttaga catgcaggga caatcccagc ttgttttaga ttagtagaga agttcgtaaa
     2761 tttccattgc gattaagtca atgctatcaa acgtatacgt ttcgcgatct tctacgtcac
     2821 gaagggtaaa gattgggccg ttttcttcgt cgtggctgta cgcaacttct gcaacaacaa
     2881 ctccttcgcg ttcaaaatta cgttttaaat ttccgccatc gtttgccatt gcttcaaggc
     2941 ggttgatgat tctaaccaaa tgtgattcca ttttgattct ccttcatttc ttatcgttac
     3001 tattttacca taaaggaggc caacttgact agaaaaaact aagatttctc gctaattctg
     3061 ctagtttaaa gatttttaaa taaattggat aaaaaattta aattttaatt caaaacatgt
     3121 tataatcata cagtattcta ttttagaaag cagtgtgact atgaattttt cttttttacc
     3181 taagtattta ccttatttta actatggggc tgttgtgacg attcttattt ctatctgtgt
     3241 tatctttttg ggaactattt tgggtgttgt cttggctttt gggcaacgtt caaagtttaa
     3301 accgcttgtt tggttggcca acttgtacgt ttggattttc cgtgggacac cgatgatggt
     3361 tcaaattatg attgcctttg ctcttatgca tatcaatgct ccgactattc agattggaat
     3421 tttaggtgtt gatttttcgc gtctgattcc agggattttg attatctcta tgaatagtgg
     3481 tgcttatgtt tcggagattg ttcgtgccgg aatcaatgcg gttccaaaag gtcagctaga
     3541 ggcggcttat tcgctaggga ttcgtcctaa aaatgcgatg cgttatgtga ttttgccaca
     3601 agcagtcaaa aatatcttgc cagcattggg gaacgaattt atcaccatta tcaaggacag
     3661 ctccctctta tcagctattg gggtcatgga gttgtggaat ggggctacaa cagtttctac
     3721 aacaacctat ctacctttaa caccactttt atttgcagca ttttactact tgattatgac
     3781 ctctattctg acagtagcct tgaaagcttt tgaaaaacat atgggacaag gagataagaa
     3841 ataatgacag aaaccttgat aaaaattgaa aatttacata aatcttttgg aaagaatgaa
     3901 gtattgaagg gcatcaacct cgagattaaa agaggagaag ttgtcgttat catcggtcct
     3961 tcagggagtg ggaaatctac cttgcttcgc tctatgaatt tgttggagga agcaaccaag
     4021 gggaaggtta tctttgaggg agtcgatatt acggacaaga agaatgacct gtttgccatg
     4081 cgtgagaaga tgggcatggt tttccaacaa tttaacctct ttcctaatat gactgtgatg
     4141 gaaaatatta ccttgtcacc tatcaagact aaaggtgaaa gcagggaagt tgctgagaag
     4201 agagcccaag agcttttgga aaaagttggt ttgccagata aggcagacgc ttatccacag
     4261 agtctgtcgg gtgggcaaca acaacggatt gccatcgcgc gtgggttggc tatggaacca
     4321 gatgttttgc tctttgacga accaacttca gccttggatc ctgaaatggt aggtgaggta
     4381 ttggctgtta tgcaagacat tgccaagtca gggatgacta tggttatcgt aacacatgag
     4441 atgggatttg cccgtgaggt ggcagatcgt gttatcttta tggcagacgg tgtggttgtt
     4501 gaagatggga cacctgagca gatttttgaa caaacccaag aacaacggac taaagatttc
     4561 ttgagtaagg ttttataatc tattttaaaa tttcaagaca agattagtga aaagttcaac
     4621 cagagctttt tcttatagtt tgaaactata ggataaccta ggaaagaagt gttagagggc
     4681 actagcagtt tttggtataa tggaagatat ttgaaagaaa agagaagtga tatgacacag
     4741 attattgatg ggaaagcttt agcggccaaa ttgcaggggc agttggctga aaagactgca
     4801 aaattaaagg aggaaacagc tctagtgcct ggtttggtag tgattttggt tggggataat
     4861 ccagccagcc aagtctacgt tcgcaacaag gagaggtcag ctcttgcggc tggttaccgt
     4921 agcgaagtag tgcgagttcc agagaccatt actcaagagg aattgttaga cctgattgct
     4981 aaatataatc aggatccagc ttggcatggg attttggtcc agttgccatt accaaaacat
     5041 atcgatgaag aggcggtttt attagccatt gacccagaaa aggatgtgga tggtttccat
     5101 cccctaaaca tgggacgtct ttggtctgga catccagtca tgattccctc gacacctgca
     5161 ggaattatgg aaatgtttca tgaatatggg attgacttgg aaggtaaaaa tgcggtcgta
     5221 atcggtcgtt caaatatcgt tggaaaacct atggctcagc ttcttttggc taaaaatgcg
     5281 acagtaacct tgacccactc acgaactcat aatcttgcca aggtggctgc taaagcagat
     5341 attcttgtag tcgcaatcgg ccgcgccaag tttgtgactg ctgactttgt caaaccaggt
     5401 gcggttgtca ttgacgttgg gatgaatcga gatgaaaatg ggaagctctg tggagatgtt
     5461 gattatgagg cagttgcacc acttgctagc catatcacgc cagtccctgg aggtgtcggt
     5521 cctatgacca ttaccatgct gatggagcaa acttatcaag cagcacttcg gacattggat
     5581 agaaaataag agaagcattc gattaataga gtgtattttc tatagctata tctaaaatgg
     5641 cagaaatgaa tattaaattt tagatataag attacaaaag gaggtctgcg tctccttttt
     5701 attgtataat aaaagtgaga ggaaaaaagg atgaaagtga ttaatcaaac cttattagaa
     5761 aaagttatta ttgaacgttc tcgttcaagt cataaaggag attatggtcg tctgctgctg
     5821 cttggtggta cttatcctta tggaggtgcc atcatcatgg ctgctttagc agctgtaaaa
     5881 agcggtgcag gattgatgac cgttggaacg gacagggaaa atatcccggc tctgcacagc
     5941 catttacctg agcctatggc cttttctctt caagaccagc aattgttaaa agagcaattg
     6001 gagaaggcag aagttatctt gctggggcct ggtttaggag acaatgcttt tggagaagat
     6061 ttagttaaac aggtctttgc tggcttaaaa cagaatcaga ttttgattgt agacggaggt
     6121 gccttgacca ttcttgctag gacaagtttg tcatttccct caagccagct tatcctaact
     6181 cctcaccaaa aagaatggga aaaattatct ggaattacga ttgaaaagca aaaggaagat
     6241 gcaacggcta gtgttctgac ttccttccct caaggaacaa ttttggtgga gaaaggccca
     6301 gctactcgta tttgggaagt tggccaatct gattattacc agttacaggt tggtggtcct
     6361 tatcaggcga ctgggggaat gggagatact ctggctggga tgatcgcagg atttgtaggc
     6421 caattccgac aggccagtct ctacgagcgt gtggcagtag cgacccatct tcattcagcc
     6481 atagcccaag aactatctca agaaaattat gtggtcttac cgacggaaat cagcaggtat
     6541 cttcccaaaa taatgaaaat tatttgtcaa caagagaggg taagcaaaga taaacttgtt
     6601 taaaatagac agataaatct acttccttct cttaatttat gattcttgaa atgcgcctgc
     6661 tttaaggcta aactagacgt atcaaagtta agagtttgtt tttgtaactg gaaagagatg
     6721 aacaaatcaa atacataaaa gataaactca tttttattcg ttttgtaaaa ataaaatgca
     6781 tatttttaaa gaaagattga tgaaaaatgt agatttgatg tttttacttg aataaaaagc
     6841 ggtttcatag taaaataaga actgagaatg atggaggtaa atcggtggaa aatctgaaga
     6901 aaatggcagg tatcaaggct gctgagttcg tgagcgatgg aatggtcgtt ggacttggaa
     6961 caggctcgac tgcctattat tttgtcgaag aaatcggtcg tcgtatcaag gaagaaggat
     7021 tgcagattac agctgtaaca acttctagtg tgaccactaa acaggcagaa ggccttaata
     7081 tcccgctcaa gtctattgac caagtagact ttgtcgatgt gacggttgat ggcgcggatg
     7141 aagtggatag tcagtttaat ggaatcaaag gcggtggtgg tgcccttctc atggaaaagg
     7201 tggtcgcaac accatcaaaa gaatacattt gggtggtgga tgaaagcaag ctggtcgaaa
     7261 aactaggtgc ttttaaattg ccagtagaag tggttcagta tggtgcagag caggtctttc
     7321 gtcattttga acgagctggc tacaaaccaa gtttccgtga aaaagacggt caacgttttg
     7381 tgacggatat gcagaatttt atcattgacc tcgccttgga tgtcattgaa aatccaattg
     7441 cttttggaca agaattggac catgtcgttg gtgttgtgga gcatggttta ttcaaccaaa
     7501 tggtggataa ggtaatcgtt gctggacgag atggagttca gatttcaact tcaaaaaaag
     7561 gaaaatagaa gggggcataa gatgtctaaa tttaatcgta ttcatttggt ggtactggat
     7621 tctgtaggaa tcggtgcagc accagatgct aataactttg tcaatgcagg ggttccagat
     7681 ggagcttctg acacactggg acacatttca aaaacagttg gtttgaatgt cccaaacatg
     7741 gctaaaatag gtcttggaaa tattcctcgt gaaactcctc ttaagactgt agcagctgaa
     7801 agcaatccaa ctggatatgc aacaaaatta gaggaagtat ctcttggtaa ggatactatg
     7861 actggacact gggaaatcat gggactcaac attactgagc ctttcgatac tttctggaac
     7921 ggattcccag aagaaatcct gacaaaaatc gaagaattct caggacgcaa ggttattcgt
     7981 gaagccaaca aaccttattc aggaacggct gttatcgatg attttggacc acgtcagatg
     8041 gaaactggag agttgattat ctatacttca gctgaccctg ttttgcagat tgctgcccac
     8101 gaagacatta ttcctttgga tgaattgtac cgtatctgtg aatacgctcg ttcgattacc
     8161 cttgagcgtc ctgcccttct tggtcgcatc attgctcgcc cttatgtagg tgaaccaggt
     8221 aacttcactc gtacggcaaa ccgtcgtgac ttggctgtat ctccattttc cccaactgtt
     8281 ttggataaat tgaatgaggc tggtatcgat acttatgctg tgggtaaaat caacgatatc
     8341 tttaacggtg ctggtatcaa ccatgacatg ggtcacaaca agtcaaatag tcatggaatt
     8401 gatacactat tgaagactat gggacttgct gagtttgaaa aaggattctc attcacaaac
     8461 ctagttgact ttgatgccct ttacggccat cgtcgtaatg ctcacggtta ccgtgattgc
     8521 ttgcatgagt ttgatgaacg cttacctgaa attatcgcag ctatgagaga gaatgacctt
     8581 ctcttgatta ctgcggacca tggaaatgac ccaacgtatg caggaacgga tcacactcgg
     8641 gaatatattc cattgttggc ctatagccct gcctttaaag gaaatggtct cattccagta
     8701 ggacattttg cagatatttc agcgactgtt gccgataact ttggtgtgga aactgctatg
     8761 attggggaaa gtttcttaga taaattggta taagatgacg cgctatgctt tgctggtgag
     8821 aggtatcaat gttggtggta agaataaggt cgtcatggcg gagcttcgtc aagaattgac
     8881 aaacttggga ctggaaaagg ttgagagcta catcaatagt ggcaatattt tctttacttc
     8941 gatagattcc aaagcccaat tggttgaaaa gctagagact ttctttgcag tccattatcc
     9001 atttattcag agcttttctt tactgagtct agaggacttt gaggcggaac ttgaaaatct
     9061 accagcttgg tggagcagag acttggcacg aaaagatttt ctcttttaca ctgagggttt
     9121 ggatgtggac caagtcatcg cgacagttga aagtttagag ctgaaagatg aagtgcttta
     9181 ttttggaaaa cttgggattt tctgggggaa attttctgaa gaatcctatt ctaagactgc
     9241 ctatcataag tacttgctga aggtgccttt ctaccgccac attactattc gtaatgctaa
     9301 aacctttgac aaaattggtc aaatgctaaa aaaataataa aggagacaca caatgacatt
     9361 tttaaacaaa atccatgaaa ctgctacttt cctgaaagaa aagggaattg cagcccctga
     9421 gttcggtcta atccttggat caggacttgg agaattggca gaagaaatcg aaaatccagt
     9481 tgtagtagac tatgctgaga ttccaaactg gggccgttca acagtagtcg gtcatgctgg
     9541 taaattggta tatggtgaac tggcaggtcg caaggtcttg gctcttcaag ggcgtttcca
     9601 tttctatgaa gggaatcctc tggaagtggt gactttccca gttcgtgtga tgaaagttct
     9661 tggatgtgaa ggtgttattg taaccaatgc agctggcggt atcggatttg gtcctggtac
     9721 cttgatggct atctcagacc atatcaacat gacggggcaa aatccattga tgggtgaaaa
     9781 cttggatgac tttggcccac gtttcccaga tatgtctagg gcctacacac cagaataccg
     9841 tgccactgcc catgaagtgg ctaaaaaact taatatcaag cttgatgaag gtgtctatat
     9901 cggagttact ggtccgactt atgaaacacc agcagaaatt cgttcctata agacactggg
     9961 agcagatgca gttggtatgt ctacggttcc tgaagttatc gtggcagccc actctggctt
    10021 gaaagttctg ggaatttcat gtatcactaa ctttgcggcc ggtttccaag aagaactcaa
    10081 tcacgaagaa gttgtagaag tgactgaacg tgttaaaggt gatttcaaag gcttgcttaa
    10141 agcgattctt gctgaattgt aagaaaaaag atttaaaagg gggagtgcct ctgttttttc
    10201 aggattgact gcctatccgg attaaagaag aaacagagga atactatgag cttcttcctg
    10261 ctcttataac tgaaagaagc ggaagaatag gtatgtctga tctgatagcc agcattgtga
    10321 aagacaagat tctaggatac tagcattagc ttcctagcca agcagactag tatgataagg
    10381 agagatgaga atgaattgac tttctgaatt tctcagtctt atcatatata gcacaatgag
    10441 atttcgcttg agtctgcttg taaataaacg aaaagaaaga taagaaataa tgaaaattgg
    10501 tcaacgaatt atgcgctttg gcataaaaaa ttaagtatcg gagttgtatc tgttgtagtc
    10561 ggctttgatt tctagctcca gctggaattt cagccaatga agtaaagcaa gatgtaacat
    10621 ctgaagtggt aataggtgtg ctagattcta aggaggaatt gaaagagtca gaaaatgatg
    10681 ctccaaaact agaaactcct cttagagagg agccaagact agctcctcaa acgcttccgg
    10741 aagcaagtga agttcttgaa aacaaaaggg aagagtcaaa agtagagata acataaccag
    10801 ctcaagcgga tgatatccgc aaggttgttg gggaattagc caaggatata agtattacta
    10861 agttgtatat gacaggtcat tctcttggat gttacctagc tcagattgca gcggttgaag
    10921 cttaccaaaa atatcctgat ttttataacc atgtattgag gaaagtgaca actttcagtg
    10981 ctcctaaagt gattacttcc agaactgttt ggaatgctaa gaatggtttc tgggatgttg
    11041 gtttggaaag tcgtaaatta gctgttagtg gaaaaattaa gcattatgtg gttgataatg
    11101 acaatgttgt gactcccttg attcataata atcgtgatat tgttacattt acaggtaatt
    11161 cacgctttaa acaccgttct cgtggctatt ttgaaagtcc aatgaatgat attcctaact
    11221 ttaatattgg taaacaagct accttggata aacatggtta tcgtgatccg aaattggata
    11281 aagtgcgatt ctttaagaaa caggctctgc ctcgatcttc tagtcaacca agcgctgaac
    11341 caatggaaaa tattgcctta ggaaaacagg ttactcaaag ttcgacagct ttcggaggag
    11401 atgctagaag agctgtggat ggcaaagtcg atggtaacta tggtcacaat tctgtcactc
    11461 atacaaactt ccaatctaag ccttggtggc aagtagattt ggctaaagaa gaaaccattc
    11521 gccaaatcaa tatttacaac cgaacagaca ctgcccagga tagattggca aactttgatg
    11581 tcattctttt agacagttct ggtaaagaaa ttgagtgaaa acgtataata tctcctaaag
    11641 atgtgtcagc acaaattacg attaaccata aaaaagcgcg ctatgttcgg attgagctag
    11701 aaggctataa tgccctcagt cttgcagaag ttgaagtttt ccgctttata gctacgaatg
    11761 ctgaaacggc gacacaagtt tctaagccag ttcaaccaat cagtcagact cctgtgaaag
    11821 ataaaacatt gacaattcaa cacagtggag cttacattgc ccgctactcc ataacttggg
    11881 aagaagttcc agtagataaa gatggaaacc aagttgttcg tagtcattct tgggaaggaa
    11941 acggtcgcaa ccagactgcg ggttttgtcc tcaacctccc aatcaaagaa aatatgagaa
    12001 atctgcgagt taagattgag aaaaagacgg gcctactatg gaatagatgg caaacaatct
    12061 atgaaaacag accaatttca gctcaacccc accgtaaaat tacccattgg ggtacgacat
    12121 tgaattccaa ggtgagtgac gatgatgtct tgtaatctga tggtacaatg acagttagtt
    12181 tgtctagttt ataagaaagt actacctgag cttgaataga actcaggtag ctctctgtga
    12241 aagaacaaaa ttaatactca atgaaaatca aagagcaaac taggaaacta gccgcaggtt
    12301 gctcaaagca ctgctttgag gttgtagata agactgacga agtcagtcac atatataatc
    12361 caaggcgacg ttgacgtggt ttgaagagat tttcgaagag tataaacaga aaggtagagc
    12421 gcgtgttcta atttgaacac gagtagaaaa cttttctaaa aacaaaaacg aaaggatggg
    12481 taaactgtat tcgctgaact gaatacgggc gactctcctc taaatcaaaa ttaagaaagg
    12541 aattgacccc accctaaaag tagtgggaaa aagatagttg gtctagcgag catcgctcac
    12601 tgcgcccaac tcctattttc ccttcgcttt ttgatgggtt tggtatcttt ctcaatataa
    12661 aataaagaaa ggtagagcgt gtgttttgat ttgaacacga gcggaaaact cggaaaatag
    12721 ataatctgac tgaaaaatca ggatttctcg tcaggttcct aattttcagt cgttttcttc
    12781 tcgctctttg tatcataaat tatgtctatc catattgctg ctcagcaggg tgaaattgct
    12841 gataaaattc ttcttcctgg ggatcctctt cgtgctaagt ttattgcgga gaatttcctt
    12901 gatgatgctg tttgttttaa cgaagtgcgt aacatgtttg gttacactgg tacttacaag
    12961 ggtcactgtg tatctgtcat gggaactggg atgggaatgc catctatttc gatttatgcg
    13021 cgtgagttaa tcgtagacta cggtgtgaag aaattgattc gtgtgggaac tgcaggttct
    13081 ttgaatgaag aggttcatgt tcgtgaatta gttttggcgc aggcggctgc aaccaactca
    13141 aacatcgttc gtaatgactg gccacagtac gattttccac aaattgctag ctttgatttg
    13201 cttgataaag cctaccatat cgccaaaaaa cttggtatga ctactcacgt tgggaacgtt
    13261 ttgtcatctg atgtctttta ctcaaattac tttgaaaaga atatcgagct tggtaaatgg
    13321 ggagtcaagg ctgtggaaat ggaagcagca gctctttact atcttgctgc ccaataccat
    13381 gttgatgcgc tagctatcat gaccatctct gatagcttgg tcaatccaga cgaagacaca
    13441 actgcagaag aacgtcaaaa taccttcact gatatgatga aggttggttt ggaaaccttg
    13501 attgcagaat aattatagcc aaaaaggaag ccctttatga attgtaggac ttccttttct
    13561 tatccagaaa ttgatctagc tctctctgat ttcgaagaat agtgacttta tgtgaatatt
    13621 cttggcaaag tttttggtaa ttttcttttt gagttttgct acgcccatcc caaagaatcc
    13681 atctgataaa ctcccactca aagcgttcag ggcaatctgc cgccatactt tctctgactt
    13741 ttccacggta tttaagataa cgcttaaagg ctctaaagag acaggtcaat ggcgaaaaat
    13801 tgagaaagat gatttggtca gcttcttgca ttcgttcttg gtagtagcac caagaataat
    13861 taccatcgat gacccaagct ttatgcttgg tgagaaagtt ttttatctcg gttaacatcc
    13921 attcgcagtc actgtcttgc caaccaggtt gaaattggag tgtgtccatg tgcagttttg
    13981 gaatggagta gtagttagat aacttttctg ctatagttga cttaccagaa ccagaatatc
    14041 cgataattgc gattttcatt ttctactttt tcctatttgg agacaaaaaa acagcctcta
    14101 tggactgttt cttatttagc aagtttagct gaaagacgag ctttatcgcg gcttgctttg
    14161 tttttgtgaa tcaaaccttt agtttctgct ttatcgatag ctgagctagc agcacggaaa
    14221 agttcttcag atgggtttgc ttcgaaagct tttatagcag tacgcatagc tgatttttga
    14281 gctgagttct tttcgttttg tttaacgttc aattcagcgc gtttgatagc tgatttaatg
    14341 tttgccaatg ttcttacctc catatttact aactatacca ttatatatga aaacttacgt
    14401 tttgacaagg gaaaattact tttttaagta aatttcatcg atttcatggt tcttgctttt
    14461 atgaagaatc acttctgcac gatttctggt tggttcaata taattttgca gatttgtgag
    14521 attgatactg atccagacct gatgggcaaa ggactccact tccccaatcg gcatctgagt
    14581 aaaacgataa tagtagctat cagggtcgtt ttgggctagg ctcagcattt tcaagaaacg
    14641 gtccagatac caactttcaa tatcatccac tccagcatct acatagatgg aaaagtcaaa
    14701 gaagtcagtg atatagagac gatcgttttg tggattttga aagacattaa ttccctcaac
    14761 aattacaaaa tcagcagctt tgacactttg tttcttttcg ggtacgatgt cgtaaacttc
    14821 atgagaatag acaggaatat ctacatcttg tccatttttg atgcggtcca agaagttgag
    14881 aagagcttcc atatcatagc tttcaggaaa tcctttacga tttaaaatcc cctgctcaat
    14941 caaggtttga ttgggataga gaaaaccatc agttgtaacc aactcaaccg tagcatctgt
    15001 aaacgtacgg gacagtagga tttgaagtag gcgactggtt gtggattttc caacggcaac
    15061 actcccagaa accccaataa taaaaggttg agatttactt tcacgttgga ggaaaattcc
    15121 ttttgaaaag gctaaatctt ccttagttcg cttgtaaatc tgaatcagat gagccaaggg
    15181 gagatagata tctgtaacgt cttggagact gatttggtca ttaaaactct tgatagattc
    15241 caattcttct tctgtcaaag gaggtgttgt ctttcgatgt aaagattgcc aagtctggcg
    15301 gctgattttt tcaaaatgta aaaattcgtt ggtcatttgt tttcccctag atattttgtc
    15361 tatcatacca taaaaagcta aaaaaataaa gcggtttctt gaatgtggta agtcaatggt
    15421 atatagtata agtgatcaat ttattgatga aataggagat agattctact gatttataag
    15481 cttataaagg gggcttttta gttcacaact tataggaggt gtactatgaa aatcttaaaa
    15541 cgttacatat tggaactctg ttttatttta agttttgctt taccttttat aaaaggaacc
    15601 aatgcagata atggtagatg ctttgtggaa acctattacg gttttacttt tttgatggaa
    15661 catgctattg taacagctgt ctttatctat tcgtttttaa ttgctttctt actaaaaaaa
    15721 cgatgggcga aatggattgc tgctggtagt tattgctttt tagtcctatg gattgcgaca
    15781 gagggttatt tcttccgcat gtcactagaa gatttgatac gactttggac aagtttggaa
    15841 atcctgacac aaacgtatca gttgggcttt tatctaaaca ttttgttagg aattttgttg
    15901 ataataaagt attttaaggt taagcaatag aaataataat ctgcctgatt atagatgata
    15961 aactttttgt ttttatctgg actgtgatat ttatagttct aaatggttta ttatttataa
    16021 gtgttgaaag agattgaaga aaatggcttc gtgacttgct ttcttcggtc tttttcgttt
    16081 tattatcaat ttgtaaacga tttacaattt gagtcaaatt atggtaaaat agtgtcatga
    16141 gtaaaatgta ttatgcagaa aatcctgacg ctgctcacga cattcatgag ttgagagtgg
    16201 acttgttggg acaaaaaatg acttttttga cagatgcggg tgtttttagc aagaaaatgg
    16261 ttgactttgg aagtcaactc ttgctcaagt gtctggatgt caaccaagga gagacagtcc
    16321 tagatgtagg ctgtggttat ggtccattag gtttgtcctt ggtcaaggtt tacggagttc
    16381 aggcgaccat ggttgatatt aacactcgtg ccttggattt agctcggaga aatgctgaaa
    16441 aaaataatgc aaaagcgacg attttccagt ccaacatcta tgaacaagtt gaaggacatt
    16501 ttgatcatgt catttccaat cctcctatcc gtgcgggcaa gcaagtcgtt catgaaatca
    16561 ttgagaaaag taaagatttc ttggaaactg gtggggattt gacaatcgtc atccagaaaa
    16621 aacaaggggc tccaagtgct aagtccaaga tggaggatgt gtttggtaat tgtgaaatcc
    16681 ttaaaaaaga caaaggatac tatatcctta gaagtgtgaa agaatgagag cagttgattt
    16741 aatacaaaaa aaacgagacg gtcaagaact gacttcaagt gaaattgaat ggctggtaga
    16801 aggctatgtg tcaggaactg ttcctgatta tcagatgtct gcctttgcta tggctgttta
    16861 ttttaaagga atgacgactc gtgaaatttc tgatttaacg atgaatatgg ttaagactgg
    16921 gcaagagttt gacctgtcag ccattgatgg tgtgaaagtt gacaagcatt ctactggtgg
    16981 tgtcggcgat aaggtaacct tgattttagc tcctcttgtt gctagcttcg gtgtgcctgt
    17041 agcaaaaatg agtggtcgtg gtcttggcca tacaggcgga acaattgata aattggagtc
    17101 cattaggggc tatcaagtag aacgtagtca agaggatttt attcgtcagg ttcaggatat
    17161 tggagtatct gtcattgggc aatcagacca gctggtcaaa gcagataagc ttctctatgc
    17221 ccttcgtgat gtgaccgcaa ctgtcgacac gattcctttg attgcgagtt cggtgatgag
    17281 caagaaaatt gcggcagggg cggatgctat tttgctagac gtgactgtcg gtgagggtgc
    17341 cttcatgaag acggttgatg aggcgcgtga gttggctcaa actatggtag atcttggtaa
    17401 ggttgttgga cggaagacag tagcagtgat tacggatatg agccagcctt tgggacgagc
    17461 gattggaaat cgtctggaaa tacttgaagc attggagatt ttgcaaggtc aaggacgtca
    17521 ggatattacc cactttattt gtgaattggc tcaaattatg cttggtttgg caaatgttaa
    17581 caagactgtt gaagaagttc accaacatct tgagaatggt caagcactgg ctaagtttga
    17641 ggaaatggtg caagcccaag gtggagactt ggaagacctc tatcgtcctg taaatgtagc
    17701 tcatgtggtg gaaatccctg ctcaggaaac gggtgtcatt tcagctcttc cagctatgga
    17761 ctttggtctt tatgccatga ggctgggggc tggtcgtgca gttaagtctg atgccttgga
    17821 ctatgaaaca ggaattgttt ttgaaaagaa gattggagac tccgttcaaa agggtgaaat
    17881 tgttgcaaaa gtatacacaa atgaaaaaat tccacctcaa ctagttacag atttccaaaa
    17941 atgtgttaaa ataagtgatg aagtgaaaaa aatccgtgaa attgtagaaa tcatctccta
    18001 aatggaggga aaaccgaaat gaaattaaat aaatatatag atcatacgct tttaaaacaa
    18061 gatgcaaaga aaaaacaaat tgatagtttg ttgtctgagg ctagagagta tgactttgcc
    18121 agtgtttgcg ttaatccgac ctgggttgaa catgctaaaa aaggacttga aggcacagat
    18181 gttaaggttt gcacggtagt aggtttccct ttgggagcaa caacttcagc cgtgaaagca
    18241 tttgagacaa aagaagctat ccaaaatggt gcagatgaga ttgatatggt gatcaatgtt
    18301 ggagctctca aatcaggtaa tttagccttg gttgagtcag atattcgcgc agtagtggaa
    18361 gcaagtggtg ataagttagt gaaagtcatt attgaagctt gccttctgac agaccaagaa
    18421 aaaattgttg tttgccaatt ggcccaaaaa gctggggctg actttgttaa aacatctact
    18481 ggtttttcaa ctggtggtgc tacgatagca gatgttacat taatgcgtga aacagttgga
    18541 tctgatatgg gagtcaaggc tgctggtgga gctcgttctt atgcagatgc tcttgccttt
    18601 gtcgaagcag gtgcgacccg tatcggaacg tcagctgggg tagctatttt aaaaggagaa
    18661 ttggcagatg gcgactactg agttgattga actggcaatt gaaaccagca aacatgccta
    18721 tgtcccctat tctcactttc ctatcggagc agttttagta gccaaagacg gaagtgttta
    18781 cacgggagtg aatatcgaaa atgctagcta tcctttgacc aattgcggtg agagaacagc
    18841 tatttttaag gctatatctg aaggccaaag agaattttca gaattgattg tctatggtca
    18901 aactgaaaaa ccgatctcgc catgtggtgc ttgtcgccaa gtgatggctg aattttttga
    18961 acaagatcta aaagtgacct tagtcgcaaa agataaatcg acggtcgaga tgacggtcgg
    19021 ggaattactt ccatactctt ttacagactt aaactagtct gagtcgctct ctgagtggca
    19081 cggtccttgt ggccaaccaa tccatacttg caacatcgtt gcacatctta tttaggaggt
    19141 tcagtaatga acaagaaaca atggctaggt cttggcctag ttgcagtggc agcagttgga
    19201 cttgctgcat gtggtaaccg ctcttctcgt aacgcagctt catcttctga tgtgaagaca
    19261 aaagcagcaa tcgtcactga tactggtggt gttgatgaca aatcattcaa ccaatcagct
    19321 tgggaaggtt tgcaggcttg gggtaaagaa cacaatcttt caaaagataa cggtttcact
    19381 tacttccaat caacaagtga agctgactac gctaacaact tgcaacaagc ggctggaagt
    19441 tacaacctaa tcttcggtgt tggttttgcc cttaataatg cagttaaaga tgcagcaaaa
    19501 gaacacactg acttgaacta tgtcttgatt gatgatgtga ttaaagacca aaagaatgtt
    19561 gcgagcgtaa ctttcgctga taatgagtca ggttaccttg caggtgtggc tgcagcaaaa
    19621 acaactaaga caaaacaagt tggttttgta ggtggtatcg aatctgaagt tatctctcgt
    19681 tttgaagcag gattcaaggc tggtgttgcg tcagtagacc catctatcaa agtccaagtt
    19741 gactacgctg gttcatttgg tgatgcggct aaaggtaaaa caattgcagc cgcacaatac
    19801 gcagccggtg cagatattgt ttaccaagta gctggtggta caggtgcagg tgtctttgca
    19861 gaggcaaaat ctctcaacga aagccgtcct gaaaatgaaa aagtttgggt tatcggtgtt
    19921 gatcgtgacc aagaagcaga aggtaaatac acttctaaag atggcaaaga atcaaacttt
    19981 gttcttgtat ctactttgaa acaagttggt acaactgtaa aagatatttc taacaaggca
    20041 gaaaaaggag aattccctgg cggtcaagtg atcgtttact cattgaagga taaaggggtt
    20101 gacttggcag taacaaacct ttcagaagaa ggtaaaaaag ctgtcgaaga tgcaaaagct
    20161 aaaatccttg atggaagcgt aaaagttcct gaaaaataat ggatggaagt catttcttag
    20221 gaagcggcca agggccgctt ttttaagagt tgagacaaag tcattttgtc tcagtaaagc
    20281 ttgctactag acaaactagc aagttttatt gaaataaaat aagactctga aaggaagagc
    20341 acatggcaca cgaaaatgtc attgagatgc gtgatattac caaggtgttt ggtggatttg
    20401 ttgccaacga caaaatcaac ttgcacctac gaaaaggtga aattcatgca cttttaggag
    20461 aaaatggggc tggtaagtcc acgctaatga acatgttagc aggccttctt gaaccaacta
    20521 gtggtgaaat cgcggtcaac ggtcaagttg tcaatctcga ctccccatct aaagcagcta
    20581 gcttgggaat cgggatggtt caccagcact ttatgttggt tgaagccttc acagtggctg
    20641 aaaacatcat tttaggtagt gaattgacta aaaatggtgt gctagatatc gctggagcta
    20701 gcaaagaaat caaggctctt tctgaacgtt atggcttagc tgttgaccct tctgccaagg
    20761 tagcagatat ctcagttgga gcccaacaac gtgtagaaat tttaaaaaca ctttatcggg
    20821 gggctgatat ccttatcttt gacgaaccaa cggctgtttt gactccatca gaaattgatg
    20881 agttgatggc tattatgaaa aatcttgtca aagaaggaaa atcaattatc ttgattaccc
    20941 acaaattgga tgaaattcgc gcagtttctg accgtgttac agttatccgt cgtgggaaat
    21001 caattgaaac cgttgaaatt gcaggggcta ccaatgctga tttggcggaa atgatggtag
    21061 gacgttctgt ttcctttaaa acagagaagc aagcctctaa accaaaagaa gtggttttgt
    21121 ctatcaaaga tttggtggtc aatgaaaacc gtggtgttcc agctgttaaa aatctgtcct
    21181 tggatgttcg tgctggagag attgttggta ttgcggggat tgatggaaat ggtcagtctg
    21241 aactgattca agccattaca ggtcttcgta aggttgaatc tggtagcatt gagctaaaag
    21301 gagattcaat tgtaggcttg cacccacgtc agattacaga actaagtgtt gggcacgttc
    21361 cagaagaccg tcaccgtgat ggcttgattt tggaaatgat gatatctgaa aatattgccc
    21421 ttcaaaccta ctataaagaa ccacatagta aaaatggaat tttgaattat tcaaatatta
    21481 cttcttatgc taaaaagctg atggaagagt ttgatgttcg cgctgccagt gaattagttc
    21541 ctgcagctgc actctcagga ggaaatcaac aaaaagcaat tattgctcgt gaaattgatc
    21601 gagatcctga tctccttatc gttagccagc caactcgtgg gttggatgtc ggtgccattg
    21661 agtatatcca caaacgcttg attgaagagc gtgataatgg caaggctgtc cttgttgtca
    21721 gctttgaatt ggatgagatt ttaaacgtct cagaccgtat tgccgttatc cacgatggta
    21781 agattcaagg tattgtatca ccagaaacaa ccaataaaca agaacttggt gtcttgatgg
    21841 ctggtggaaa cttgggaaag gagaagagtg atgtctaaaa aattacaaca aatttcggtt
    21901 cccttgattt ctgtatttct aggaatttta ctcggagcca ttgtcatgtg gatcttcggt
    21961 tatgatgcta tttggggcta cgaagaattg ttctatacag cctttggcag tctgcgtggg
    22021 attggagaaa tcttccgtgc tatgggtcct ctggtcttga ttggtcttgg ttttgcggtt
    22081 gccagtcgag ctggtttctt taacgtcgga cttcctggtc aggctttggc aggttggatt
    22141 ctcagtggtt ggtttgcgct gtcgcatcca gatatgcccc gtcccttgat gattctagca
    22201 accatcgtga ttgccttgat tgctggtggg attgtcggag cgattccagg tattcttagg
    22261 gcctatctag ggacgtcaga ggttattgta accatcatga tgaactacat tgtcttgtat
    22321 gtagggaatg cctttatcca tgctttccct aaagacttca tgcaaagtac agattcgacc
    22381 attcgtgttg gggctaatgc aacctatcag acaccttggt tggctgagtt gactggtaac
    22441 tcacggatga atattggtat tttctttgcc atcattgccg ttgcagttat ttggttcatg
    22501 ctcaagaaaa caactcttgg ttttgaaatc cgtgcagttg gtcttaatcc acatgcttca
    22561 gaatatgctg gtatttctgc caagcggact attatcctat ctatgattat ttcaggtgcc
    22621 ttggcaggtc ttggtggagc tgttgaaggt ttgggaacct tccagaacgt ctatgttcaa
    22681 ggttcgtcat tagctatcgg atttaacgga atggcggtta gtttgcttgc ggccaactca
    22741 ccaattggta tactctttgc agccttccta tttggcgttc tccaagttgg ggctcctggt
    22801 atgaatgcgg cgcaggtacc atctgagctt gtcagcattg taacagcgtc tattatcttc
    22861 tttgtcagtg ttcattacct tatcgaacgc tttgtcaaac cgaaaaaaca agttaaagga
    22921 ggtaagtaaa gatgtctatt ataaccttgc tccccctctt ggtgtcttct atgctgattt
    22981 actcagcacc tctcatcttt acaagtatcg gtggtgtttt ctctgaacgt ggtggtgtgg
    23041 taaacgttgg ccttgaagga attatggtta tgggtgcctt ttctggagtt gtctttaacc
    23101 ttgaatttgc agaacaattt ggagcagcaa ctccatggct atccttgctt gtagcaggat
    23161 tggttggtag tgttttctct atcatccacg cagcagcgac ggttcatttc cgtgcagacc
    23221 atgttgtcag cggtacggta ttgaacttga tggcgcctgc cttggctgtt ttcttagtta
    23281 aagttcttta taacaaagga caaaccgaca acctaagtca aacttttgga cgctttgatt
    23341 tcccagtctt ggcaaatatc ccagtgattg gtgatatctt cttcaagtca actagtctac
    23401 ttggttatct ggcgattgcc ttctcattcc ttgcttggtt tattctcttt aagactcaat
    23461 ttggtcttcg tctccgctct gtcggtgaac atcctcaagc agcggatacc ttgggaatca
    23521 acgtctacaa gatgagatat ttagggatta ttatttcagg ttttctaggt ggaattggcg
    23581 gagcgattta tgcgcaatca atctcagtta acttctcagt gacaactatt gttggacctg
    23641 gatttatcgc ccttgctgcg atgatctttg ggaaatggaa tccaatcggt gctatgcttt
    23701 ctagtctctt ctttggactt tcacaaagtt tggctgttat cggttctcaa ttgccgttcc
    23761 tacaaggagt gccagcggtt tatcttcaaa ttgcacctta tgttttgaca attcttgtct
    23821 tagcagcctt ctttggaaaa gcagtcgcac caaaagcaga tggtatcaac tacatcaaat
    23881 caaaataagc atacaaaaaa acgtcagtcc aatgactgaa ctgcccccca aaagttagac
    23941 agaaaaaatc taacttttgg ggtg
//
LOCUS       DAVBCV010000028        23803 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBCV010000028 DAVBCV010000000
VERSION     DAVBCV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.028"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(72..761)
                     /gene="rplA"
                     /locus_tag="VQ516_001133"
     CDS             complement(72..761)
                     /gene="rplA"
                     /locus_tag="VQ516_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2511583.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMGLVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(968..1393)
                     /gene="rplK"
                     /locus_tag="VQ516_001134"
     CDS             complement(968..1393)
                     /gene="rplK"
                     /locus_tag="VQ516_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2511584.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAVRMIEGTARSMGFTVID"
     gene            complement(1516..2232)
                     /locus_tag="VQ516_001135"
     CDS             complement(1516..2232)
                     /locus_tag="VQ516_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2511585.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTNGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYFGFEGGDYVD"
     gene            complement(2235..2705)
                     /locus_tag="VQ516_001136"
     CDS             complement(2235..2705)
                     /locus_tag="VQ516_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2511586.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVSELHHLEKKTRIRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGYGLNGRGPVWWIPFEEMTAEICQSKPDEIKRLVERLSSEVDKLLEIKE"
     gene            complement(2707..3744)
                     /locus_tag="VQ516_001137"
     CDS             complement(2707..3744)
                     /locus_tag="VQ516_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2511587.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVLGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAMLLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTNEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(3842..5167)
                     /gene="brnQ"
                     /locus_tag="VQ516_001138"
     CDS             complement(3842..5167)
                     /gene="brnQ"
                     /locus_tag="VQ516_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2511588.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5338..5430)
                     /locus_tag="VQ516_001139"
     CDS             complement(5338..5430)
                     /locus_tag="VQ516_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2511589.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5473..5625)
                     /locus_tag="VQ516_001140"
     CDS             complement(5473..5625)
                     /locus_tag="VQ516_001140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2511590.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(5785..6363)
                     /locus_tag="VQ516_001141"
     CDS             complement(5785..6363)
                     /locus_tag="VQ516_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2511591.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPCT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6417..7817)
                     /gene="pepV"
                     /locus_tag="VQ516_001142"
     CDS             complement(6417..7817)
                     /gene="pepV"
                     /locus_tag="VQ516_001142"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2511592.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(7830..8435)
                     /locus_tag="VQ516_001143"
     CDS             complement(7830..8435)
                     /locus_tag="VQ516_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2511593.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDLKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPRYRLPVDEIIEKR"
     gene            8606..9406
                     /locus_tag="VQ516_001144"
     CDS             8606..9406
                     /locus_tag="VQ516_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2511594.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9588..10421)
                     /locus_tag="VQ516_001145"
     CDS             complement(9588..10421)
                     /locus_tag="VQ516_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2511595.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWNDCVLEALDGQYGLEDPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(10411..12258)
                     /gene="uvrC"
                     /locus_tag="VQ516_001146"
     CDS             complement(10411..12258)
                     /gene="uvrC"
                     /locus_tag="VQ516_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2511596.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGIHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12298..12999)
                     /locus_tag="VQ516_001147"
     CDS             complement(12298..12999)
                     /locus_tag="VQ516_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2511597.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKMGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13078..14310)
                     /gene="murN"
                     /locus_tag="VQ516_001148"
     CDS             complement(13078..14310)
                     /gene="murN"
                     /locus_tag="VQ516_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2511598.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14315..15535)
                     /gene="murM"
                     /locus_tag="VQ516_001149"
     CDS             complement(14315..15535)
                     /gene="murM"
                     /locus_tag="VQ516_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2511599.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(15647..16426)
                     /locus_tag="VQ516_001150"
     CDS             complement(15647..16426)
                     /locus_tag="VQ516_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2511600.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16495..18156)
                     /locus_tag="VQ516_001151"
     CDS             complement(16495..18156)
                     /locus_tag="VQ516_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2511601.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKILSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18331..20553)
                     /gene="recJ"
                     /locus_tag="VQ516_001152"
     CDS             complement(18331..20553)
                     /gene="recJ"
                     /locus_tag="VQ516_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2511602.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            20830..21588
                     /locus_tag="VQ516_001153"
                     /pseudo
     CDS             20830..21588
                     /locus_tag="VQ516_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21628..22392
                     /locus_tag="VQ516_001154"
     CDS             21628..22392
                     /locus_tag="VQ516_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2511603.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22405..23082
                     /locus_tag="VQ516_001155"
     CDS             22405..23082
                     /locus_tag="VQ516_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2511604.1"
                     /translation="MIDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCMGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23092..23751
                     /locus_tag="VQ516_001156"
     CDS             23092..23751
                     /locus_tag="VQ516_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2511605.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNTVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
BASE COUNT         6727 a         5536 c         4393 g         7147 t
ORIGIN      
        1 catgcccgtc gtacatcaaa aaaagttgag acatagcgtc ttcaactttt ttacattact
       61 ttaactactg attaaagtga gtttacgtca actttgatac caacaccttg agtagttgtg
      121 atagtcaagt ttgttacgta agttccttta gctgtagctg gttttgcttt ttggattgtt
      181 tcgttgaaag ctttaaagtt ttcaaccaat ttttcagctt caaatgatac tttaccgatg
      241 attgcttgaa cgttacctgc acggtcagca cggtaagtga ttttaccacc tttagactct
      301 tcaactgctt tagcaacatc cattgttaca gtaccagttt tagggtttgg catcaagtta
      361 cgtggtccaa ggacacgtcc aagacgtcca acaagaccca tcatatcagg tgtagcgata
      421 actacgtcga agtccaacca accgtcgttg attttagcaa caaggtcatc ttcaccaaca
      481 aagtctgcac cagcagcttt tgcttcttca gcttttgcac cacgtgcgaa aacaagaacg
      541 cgtgaagttt taccagtacc gtttggcaat accattgctc cacggatttg ttgatcagct
      601 tttttaacgt cgatgttcaa gttgtaagca acttctacag ttgcgtcaaa ttttgcaaag
      661 tttgtttctt ttgcaagtgc tacagcttct tctacgctgt atgcttttgt gctgtcgatt
      721 ttctcaagag cagcacgaag ttgtttgctt tttttagcca ttttctattc tccttgtaag
      781 tggttcaatc gattttcatc tcccacgtca cttctcgaaa tgatgaagtc ttgcgggttt
      841 tcagtctatt agattctttt cctacggcct taagctccct tgctgtaccc aagtacaagc
      901 atcagtcact tgcctagtat gaaaagcaac taatagactg ctgtgagatt ttctactgtg
      961 ttattgatta gtcaataaca gtgaatccca tagaacgagc agtaccttcg atcatacgca
     1021 ctgcagactc tacgtttgct gcgttcaaat ctggcatctt agtttctgca atttcttgta
     1081 cttgtgcacg agtaactgta gcaactttag ttttattagg tgtacctgat cctttttcaa
     1141 cacctgcagc ttttttcaaa agaacagcag ctggtggtgt tttcgtaacg aaagtaaatg
     1201 atttatcttc gtaaactgag ataacaactg gaatgatcat accagcttgg tcagctgtac
     1261 gagcgttgaa ctcttttgtg aatcccatga tgttgatacc agcttgacca agagcaggtc
     1321 caaccggtgg agctggtgta gctttaccag cagggatttg caattttaca agtttttcga
     1381 cttttttagc catttttaaa tcctcctttg tggttttggc ggtaattaga gatttttacc
     1441 tcccacaagt atgtttttca catacccatc tattatacac tatttatctg gaattgcaag
     1501 agaaaagttt attttttaat cgacgtaatc tccaccttca aagccgaaat actcttccaa
     1561 actgaggcca ctagctgcaa tctctttagc ctcttttccg acataacgaa ggtgccattc
     1621 ctcagccata tagcctgttt ccttttcctt acctttgaga taacgtacaa caaagccata
     1681 atcagccgca tgatccaaga gccattgggc cgctttttct tctgtcacca aatcaccatt
     1741 tgtcccaatc acatcaaagg ctaaacctgt ctggtgctcg ctatagccag gacgggcaga
     1801 ataacggtcg gcagcttctt tcccatcttg attgacataa tcttgataga gcttggtctg
     1861 agtttcataa cttctaaagc cactgtaatg atcgctaatg gggaaacctg cctcttgcat
     1921 agctttgatg agcttgacca actcagcctt ggctgttgga ttttcccctg gattgtagtc
     1981 ttttgacaat ggatagtgtt tgttggctac gatgatttca tcgtatttcc cttgaatgct
     2041 gtaatagtct cctttgttaa ccacttctgc ttttttctgg gcagctcctt gagacttact
     2101 tcctactgtt ccatcagctt tagcagtttg ctctgtctta gctgctccat cttcattttt
     2161 tgctttttct tgtgaacaag ctgctagact caaggctagc aaggctgtca agacaaggta
     2221 tctttttttc atctttactc ctttatttct aatagtttat ctacttctga tgataaacgt
     2281 tcgactaatc ttttaatctc atcaggtttt gattggcaga tttctgctgt catttcttca
     2341 aagggaatcc accatactgg accacgtcca ttgaggccat aaccattcat atcacctctt
     2401 cgtctaggaa acagatgcca atggagatga gcatcgccat ttcctagtag ttcgatattc
     2461 attttctcag cagcaaaggc cttggcaact gcctcttgga ccacactcat ttcttctaga
     2521 aaacgtattc tagttttctt ttctaaatga tgcaattcgc tgacatgttc cttggctaga
     2581 aagagactat agcctgcaaa atactggtgg tctccaatca caagatagcc tgtttccaac
     2641 tctttgacaa aatagggatt ttctcccttc ttgatgagct caattctctg gcaaatcaaa
     2701 cacatattag tctaccaatg cactcttaag ataggcatcg accattcgtt ctgttgcgac
     2761 caccgagtca atatgagtgc gctcataaga atgactagac tcgatacccg caccaagaag
     2821 ggcatgcttg acctctgccc ctgcagacat agccgctgaa gcatccgaac cataaaatgg
     2881 atagatgtcc agcttaaaag gaatatcttg ctctttagcc aaggcaacca agtgttgacg
     2941 gaagtcatag tgataaggac ctgaggcatc cttgacacag atagatactg tgtactcatt
     3001 tgtctgctgg tcatctccca tagctcccat atccacagcc agatattcta ctacctgagc
     3061 aggaatgtta gaattagcac catgtcctac ttcttcaaaa actgaaaaag caaaatgagt
     3121 tgttactggc aattcaatct tctctttctt ataaatacga agcaggttga gcaacatcgc
     3181 tgcgctgacc ttatcatcca aatgacgaga cttgataaaa cctgtatcag tcacaacagt
     3241 tcgtggatcg aagctgataa aatcaccgac ctcaatccca agagcacgag tttctttttc
     3301 attgcttact tttacatcca aacgcacttc catattgtcc tgcgtgcgtt ctgcagttcc
     3361 tgcatccttg tagacatggc aagaagtttg gtggatgagg atggttccta ataccttttg
     3421 acctgtgcta gccacatgaa cggtacagtt ttcaccttca atcatgttcc aaggaaaacc
     3481 accgatacgg tccaatttaa gacgaccatc tggtttgaca gcacgaacaa tagcacccag
     3541 tgtgtccaca tgggcagtca catagcgatg ttgctcatca ttttgacctt tgatggtcac
     3601 attgacacca cccttggctg tgcgaactgg ctggtaacca aaaccttcta gagtcttgac
     3661 taaataattc gaaatttcac gagtgaagcc cgttggcgat gcgatggctg tcagttcttt
     3721 gatatattct actgtttgat tcatttcttc acctctttcg ctacctatta taacacattt
     3781 atcatcggat aaaaaaagaa aatcactcct gtagacttgg ctacaaaagt gatttaagtg
     3841 attattccat ttcaaaaaca tcgctttctt gcttgtttgg tagaaccaat gagagcaaga
     3901 ttccaacaat ggctggcacc aaccatggga gagatgcctt ggcaaaagga agaacgctaa
     3961 caagatttgc aagaaactca accttaaacg agcttcctag tacgcttgca atggcaataa
     4021 ctgtaaccac agcaattgtc aactgcatac ctggttttga aagggcaaca aatttgttga
     4081 caatgacaat cataacgata gcaatcgtga ttgggtacaa gataaccagt actggaattg
     4141 agtacttgat aatcgcatca agacccaaat tggcaatagc aaatccaatc aaggtaaagg
     4201 ctgtcgcata aaccttgtag ctgatttgtg ggaagcgctc attaaagaac tcagctgttg
     4261 acacaatcaa accaacagtc gttgtgaagc aggttacggt aaccatagct gcaaggaaga
     4321 gttgagctgt tgagccaaag atttcttgag tggcttgtga caagatgtaa acacctggtg
     4381 ttccaccctt catcgcttca gctggtactg ggaaatgatt tccaagaaaa cctaaaccga
     4441 tgtaaagagc gctgaaggca agggcaacaa cgataccaac aacccaaata gttgaaatgt
     4501 attctttctt acttgaaaat ccaagttgtt tcaaggtttg aactgcgatt acgctaaagg
     4561 ccactgaggc aagggcgtcc aaggtattat aaccttctag gaaacctgta ccaaaggcag
     4621 aagcttgata agcagctgaa gcagcttgag gacttgttcc accatatttg atagctccca
     4681 gaacgaccaa gataacaatc aaaattgcaa agactggcgt taaaatacgt ccaatgcggt
     4741 ctaagatttt tgatggatta agcgaaatca aataggctgc cgcaaaatac agaaccgtaa
     4801 atacaatcaa gccaagtcct ttatttgcat ccgacaaaag ggggctaatc cctacttcgt
     4861 aagctgttgt agcagtacgt ggggtagcaa agaatggacc gattgacaag taaagaactg
     4921 agaggtaaag agtcgcaaac caaggcgcta tcttcgttga aatctcgtag atatatcctt
     4981 taggatttag cgttccaata ataagggtca agacggcgat accaacgcct gaaaagacaa
     5041 aacctgcgat ggcaggaaga aaatgttctc cagatagagc acctagagaa ggcggaaaaa
     5101 tcaagttccc cgcaccaaaa aatattccaa acaggagcaa acctgttagg gtaccttttt
     5161 tagccataaa aatctccttc atatttataa aatactgatc tagtatacca cgattaggag
     5221 tctgaaaaaa gcatcacgag gcaaatatta aatgttttca attttcttaa aaataagaat
     5281 tatctaaaaa taaaactctc ctacccagtc cacaccaagt aggagaaatg caaaatttta
     5341 aagttcttca aaggctaaca tgccaccctg gacattgata acattataac cttgttctaa
     5401 taggaattgg caagcacgcg ccgatctcat tccagatttg caaataatat aatgcaagcg
     5461 atctttgtcc aattaatcat aactatcagc caattgacta agcggtaggt tgtgggcacc
     5521 ttctaaatga agagctgcaa actcatccac ttctctcaca tccactagag aaagctggtc
     5581 gttttggtaa agctggtaaa atgcgtcaaa ggctatttct ttcattcttt ttcttctaat
     5641 actcttcgaa aatctcttca aaccacgtca gcgtcgcctt accgtgcgta tgttaccgac
     5701 ttcgtcagtt ttatctgcaa cttcaaagct gtactttgag cagcctacga ctagcttcct
     5761 agtttgctct ttgattttca ttgactataa aatggtttta attctttttt tcaaatctgg
     5821 cactacttct gcctcaaacc aaggattttt ggccatccag atttgatttc gtggtgatgg
     5881 gtgaactagc ggaaaatagg ctggcagata gtctttatag tgtttcaccc tctccgttac
     5941 cttcccactg attttctcct gtaaatagta ggcttgggca tattgcccaa tcaagagggt
     6001 taactgaata tcaggcaatt cctgtaagag ctgcggatgc catttttctg caaaacctgt
     6061 acaaggcgga agatcacccg acttgccatg tcctggaaag tagaaatcca taggcaaaac
     6121 agcaaaataa cctgaattgt aaaaggtatc ttcatccaca cctagccagt cccgcaaacg
     6181 gtcaccactt ttatctttcc agtaaaggcc tgcttcttga gttttaagtc ccggagcctg
     6241 accgatgata ttgatgcgag cagtttttgg cgctgcaaag agaggctcaa tgccacgctc
     6301 tgtatagctg gcattctgcg aatccgccat gatagcctgt ttgattcttt cgatttgaga
     6361 catctacttt ccctccaaaa agaagtccaa gcataagatc tcagacttct atcgttttat
     6421 ttgatcaatt cgtaaatagc ttcggcataa attgctgctg ctcggaagag atcgtccaag
     6481 gcaataaatt cattggcttg gtgcatggta tcaattgagt ctgggaacat agcaccatag
     6541 gcaactccgc gctctaacaa gcgaccaaag gttccaccac cgatgacttg ttcatgacct
     6601 ttaaggcctg tttgtttttc atagacattc aacaaggttt gcacaagtgg atcttccatt
     6661 ggcacatagt gaggggtatg accatgttct gatagactga cagaagcaac tggcaagttt
     6721 tctaggattg acttgatttg ttctggactt gttccttttg gatagcggat gttaagggca
     6781 atggtattat cagcacttgt ttcatcgaag cggaagacgc cggcattcat agaaagagca
     6841 cccatctttt catccacatg agcaatcttg agattttcac cctcatgatc gttcaagaga
     6901 attttacctg cgatgtcaag gtagtctttg gctggaccag caaagccaaa ctggctgagg
     6961 aagagggcaa ggtaagttgc gccattgaca cctgaagcag gcatagcacc gtgggctgat
     7021 ttaccaatga tcgtcacctt gtattggcca gcttcttctt ggagttctcc tctaagtttg
     7081 tgttctgcaa caaaggcatc tagtttagct tgcaagtctg tcaagtcacc tgaaacgact
     7141 gctgttgctg attctggtac catattttca cgtaaaccac ctgtaaagct gtgaagacgg
     7201 gctgcacctg tattttcacc tgcaaagtgg aggtattccg tgatgtttcc tttttcacca
     7261 ttgatgattg gaaattcagc atctggtgag aaaccaaagt ctggcttggc gagtcctacg
     7321 tgctcgaagt agtagtccat atctgcccag cctgattctt cgtctgttcc aacgatgaag
     7381 cgaactttct tagaagttgg aaggcccaat tctttgatga ttttcaaacc atagtagcaa
     7441 gctgttgtag gtcccttgtc atctgaagca ccacgcgcat aaaggcgacc atctttgata
     7501 gttggtgtgt aagggtctgt gtcccaaccg ctaccagctg gaaccacgtc catatgggca
     7561 aagattccga gaacttcttc tccatcacca aactcaaaat gtcctgcata gttatcaaca
     7621 tttttagttg gataaccatc acggtctgcg atttcaagga atttttccaa ggcttttact
     7681 ggaccaggtc caaatggatg ttgggcatca gccttgctat catcacgttc tgagttgatt
     7741 tccaaaaggc taaacaagtc agccaagagg tcttctttgc gtttttctac ttctgctgta
     7801 aaatcaattg ctatcatttt ttcttcttcc tatctttttt cgatgatttc atctactggc
     7861 aagcggtatc ttggttccaa tttgtcgtct gtgtaaccca ctgtgatcaa gagttctggg
     7921 cggaaacggt cttcaatttc caaaacctca ttgacttttg atttgtcaaa accaaggata
     7981 atattggaac caattccttg gtctgtaaga gcgagaacca agttcatagc aaccaaacct
     8041 gcattgagtg ctaggtagtc gctgacttgt tgttcattgt agcgtgcaaa ttcagctggc
     8101 aaatttttca tgaagtattg aagttgttct tctgagaaat tgttagcacc accaactcgg
     8161 gcaatcttac gagcgcgttt ggcaaggtct gtatctgtaa atagggcaat ggttacaggt
     8221 gctgatgata cctgctcaaa gtttgaacca taagccaact ttgccagttc agcatttttc
     8281 tcacgaacaa ctacaaattt ccaaggctgg ctgttgtggg cgcttggcgc caaggttgca
     8341 atttcgatag ccgtacgcac atcttttaga tcaacaggct tatcagtgaa atgcttggtc
     8401 gcatgacgtt ttttatttaa ttcaagaaat ttcataatcg atttcctttt ctaattctac
     8461 tgcttccatt ctaccataat ttgaaagagc ttgcagggat ttttcatgat taagtttttc
     8521 tatcacagac ataaaagata tatattggtt tatcaggaaa gtttctgata aactagcaag
     8581 tactttacat ttgaatggag acagtatgaa aaaatttagc ctattactag ctatcctacc
     8641 atttttggtt gcctgtggga atcaagctac acccaaagag actagcgctc aaaagacaat
     8701 cgtccttgct acagctggcg acgtgccacc atttgactac gaagataaag gcaatctgac
     8761 aggcttcgat atcgaagttc taaaggcagt agatgaaaaa ctcagcgact acgagattca
     8821 atttcaaaga accgcctggg aaagtatctt cccaggactt gattctggtc actatcaggc
     8881 tgcggccaat aatttgagtt acacaaaaga gcgtgctgaa aaataccttt actcgcttcc
     8941 gatttccaac aatcccctcg tccttgtcag caacaagaaa aatcctttga cttctcttga
     9001 ccagatcgct ggtaaaacaa cacaagagga taccggaact tctaacgctc aattcatcaa
     9061 taactggaat cagaaacaca ctgataatcc cgctacaatt aatttttctg gtgaggatat
     9121 tggtaaacga atcctagacc ttgctaacgg agagtttgat ttcctagttt ttgacaaggt
     9181 atccgttcaa aagattatca aggaccgtgg tttagacctc tcagtcgttg atttaccttc
     9241 tgcagatagc cccagcaatt atatcatttt ctcaagcgac caaaaagagt ttaaagagca
     9301 atttgataaa gcactcaaag aactctatca agacggaacc cttgaaaaac tcagcaatac
     9361 ctatctaggt ggttcttacc tcccagatca atctcagtta caataacact cttcgaaaat
     9421 ctcttcaaac cacgtcagct ttatctgcaa cctcaaagct gtgctttgag caacctacgg
     9481 ctagcttcct agtttgctct ttgattttca ttgagtataa gatatactaa aagcgattgg
     9541 accagccaat cgctttttta gtttgcattg gctattttag gaaactctta caaaaaatca
     9601 aaaaaattct tcacatcctc tacataccca tgcttttcta ttaagctggc taaaagttcc
     9661 agattgtgcc cctgatagtt atcgtcacga cgcagttctt catacatttc aataaaggga
     9721 agccccttgg acttggccaa ttctaactca gcctcgtaat cataagcaac tttacgaaat
     9781 tggatattga ccaattcccc atcttcaact tctatcacgg catactgggc acggtgattt
     9841 tttaacgcct cccaattaaa atagggcatg ccaatcgacc ctggattgat gatttgttgc
     9901 ccttgacttc cataacgaag caactgcttg tgaacatgac cataaactgc cacgtccgtt
     9961 tccgcatcta gcagttggtc aaatttctct gtatcattct caactagcaa gtcaccacca
    10021 tagttcttat ttggcaaatt atgagagata gaaaagcgca gtccagcaat ttctttcttt
    10081 tccagcaaag gcaagcttcg tagccagaca gtcgttgcag gatccattcg ctccatcaaa
    10141 aactgagtca ttcgcatgag ctggatttcc tgtgggtctt ctaagccata ttgcccatct
    10201 aaagcctcaa ggacacaatc attccaattg cctcgaacac ttgccgtgat aggaagttcc
    10261 tttaacagag cgaccaagtc atttgcacct gaaccaggga ggaaaatgtc tcccatgagc
    10321 caatattcac tggctccttg agttttagca tctgcaatca ctgcttctag cgccgtcgca
    10381 ttgccatgaa tatctgataa aattgcgatt ttatggttta ttgtggtttc cttccgtttg
    10441 gtaatctact ctttcttccg ccacttgaag caagatttct gtttcctgcg ggttcaactt
    10501 tctttgcaca gcctctgcta ctgctcttgg tactccaact tcgacaatct catccacact
    10561 ggcttccttg attttggtca gagacttgaa gtgcctcatg agattttgct tgcgtttagg
    10621 tcccagacca tcaatcccat ccaattgaga tgagaaagaa tttttggagc gcagttggcg
    10681 gtggaaggta atggcaaagc ggtggacctc atcttgaatg cgttggagga ggaaaaattc
    10741 ctgagaattg cgagacaagt ccaccacttc cagcggatct ccaaagagca attcatgggt
    10801 ttggtgctta tcattctttt gcagacctgc aattggaata tccaagccca gttcttcttg
    10861 gatgacttgc ttagcaatat tgacttgacc ttgcccccca tcaatcacaa tcaaatctgg
    10921 aggagtcaaa gcctcacgct gtactcgacc atagcgtctt cgaatgactt ctctcatact
    10981 agcatagtcg tccggcccaa caacggtttt tatcttgtac ttacggtaat cttttttact
    11041 tggcctgccg ttaacaaaga ccaccatggc tgaaacagga ctggtcccca tgatgttgga
    11101 gttatcgaag gactcgatgc gaactggagt cggaatttgg agcaaacgtc ctagattttc
    11161 aatagctcct tgggtctttt cgactgattt ttctagcagg ttaaatttct gctccagact
    11221 aacacgtgca ttttttatgg ctagattgac cagctgtttt ttctctccac gttggggttt
    11281 gagaatcttg gtatccacca aagccttgac agcttcttcg tcaatatcct gcggaatcag
    11341 tacctcattg ggaactaggt gagatttttc ttgatagaat tgtcccacat aggtcaagaa
    11401 atcctcatct ggatcattga agtaggggaa gagattgaca tcgcgctcga tgagcttgcc
    11461 ctgacgaaca aagaaaacct gcacacacat ccagccctta tccacatagt agccaaagac
    11521 atcgcgattt tggagatcct tagccatgac ccgttgcttg gttcgaagcg ttccaatagc
    11581 ctgaatcagg tcacggtatt ccgccgcacg ttcaaactcc atactttgtg ctgctactgc
    11641 cattttactc ttgagatcat cgatgatttt gtcatcctga cctttcagaa aatcagacac
    11701 ctcctgagcc atagacttga aataagcctc atccttctta cagatggtgt gggccataca
    11761 ctggccgata tggtaataaa aacagacctt agagggcggg ttggtacatt ttcgaaaagg
    11821 gaaaatccta tctagaagtc gcttgatttc attggctgcc cccacatctg gataagggcc
    11881 aaaataaaga ccaccgtcct ttttgacctg acgagtaatg ataagccgtg gatagcgctc
    11941 attggtgatt ttgatgaaag gataggactt gtcatccttg agcatgatat tgtacttggg
    12001 cttattttcc ttgatcaagt tgatttccag cagaagcgcc tcaatattgg actcagtgac
    12061 gataaattca aaatccacaa tttcagacac cagagcctct gtcttggtat catgaattcc
    12121 acgaaaatag gaccgtactc gattacgcag atttttagcc tttcctacat agataatggt
    12181 gccattttta tccttatgaa tgtagcaacc agggctggtc ggcaagagct ctagttttga
    12241 tttaatcaag ttgttcatag ttccattata gcaaaaaaag tagggaaaga tagtacccta
    12301 ctcactagtc tcatatattt ttcggaggtt tgcaacatcc tcttcctgga taccataaaa
    12361 ggaacctgct ggttgcatga cagacttctc atctgtatgg tccaagccaa tcgcatgacc
    12421 taactcatgt tctgccgtat ggacaaggcg ttcataggag tagccatagt atggattgga
    12481 caaataataa tgattcaacc gcaccgttac ggacaagaat tgccctgtta agagattagt
    12541 ttgactttcc gcctctcctg ccacaggagt gcctccgtcg ttcatctccg tagccgtaat
    12601 atccgcctta ctagactcag tcacgagttc aaagttaaaa gcaccagttt gattccagtt
    12661 ctggattgct tctaaataag cctcttgaaa gcgtgaatcc atctgcggat ccaggtaaat
    12721 acgagcagag gcctgtgccc accttccttc agaatgctgg tgactatctg tctggcttaa
    12781 cttaggcagt tgccctgttt tctcccattg gtcaatactt tgttgaccca tttttactga
    12841 ccgttctgct tgctttagcg ctccttgaaa atccccgttc agataccaga gaagtccgaa
    12901 agcaagcaca cataagagca caactatcca aaccagacgc caaaacaaac gccacacaaa
    12961 agaaaagaaa gcccctatca aacgaaaaag ccagcgcatg tctctccacc tttctagcaa
    13021 ataagaatta ttttactaaa agaagctaaa agaaatctaa atactgactt tttcatctta
    13081 acgtcctact atttttttga gtaactggat agctttgtat tttaaaggcg atggatggta
    13141 acggaaagta cctgctttgc gtacaatata gccattaaaa ttctgtttaa aacgcaaaac
    13201 accatcactt ccatcaaaaa tcccttgaat gcctaggaag ttgtatttag gtattccacg
    13261 ttttatgctt tccaacataa catatttttg aagcagtgca ggggcataga acttattaaa
    13321 ctcagtgtag gaaccactaa agagataagt cgtttcctga ggcatataaa caaataaact
    13381 cccagctaaa acaatatctt cttctccata tttttcaatc aagtctcgcg cttctgcttt
    13441 tcgaacttca aacgtttcaa attgactaga atattctctc agttgatttt gttttttctc
    13501 agaatgagga tttttactca aatcaagtcg caacttgtcc aagttttctt ctagtttact
    13561 ttgttcacct tgcaatttgc tcatatactc cgaaaaattc aagcttgcta tgagaaactc
    13621 cgcttgttct ccaaaagtat cataaaaatg ctcataatat tctaaacttt tatcactata
    13681 ttctctacgt tcagaggttt cttttgttat attcttaaaa atcgatagtt cttcacgttt
    13741 taactttttc aaccgaatgc caaaggtttc agcctttttc accaagggtt tacccttttt
    13801 gctaaaactt ttaagcaaac tcttttcagt taattcagtt aaatctttat agtataacca
    13861 atctggttct ccacctgggt aacctgttgt taagccatca aattgataac ctaaatcagt
    13921 caaaccttga ataatacttt ttttctcagc atctattgga ttaccttggc tatcaaaagt
    13981 ttgataagtt tcataaggtt ttacaagcaa ctctaataca ccattttgct tggcatattc
    14041 ttttaactct gcataaaaaa ctggaagagc atcttgttgg gtataaatcg gccccgaatt
    14101 gagttccata tgcagaccac ccagcatggg caagctataa accagagctg caacttgaat
    14161 ttctccttct tgtttcaaag caagataaac aattcgagcc cctctttttt ctagcaaatc
    14221 ccccatctgg acagattgca taaaggaacg agaagaaacc tgatcagaat aagtctgaaa
    14281 ctcttctttc gtgagtgttg ttagtgccat atacttactt tctatgtttt tttcttaatg
    14341 ttttacggaa atcaagagca agtcttaaca gaggatagag aggatgagtg ggcattgtaa
    14401 attcacccaa gtattcttca atcgttggat taaatttttc cttaaaatga taaagtccac
    14461 cattgagaga gttttcaaca ccacctaaat tttgccagac catacctcgc tcaaaggcat
    14521 agcgagccgt ttcataccat gttaaaattg gtgcattgta acgtttaaaa tcatcatcca
    14581 taccagcata tatattgaca gaggtagtac caaattccaa actcaaagta gccgctaaag
    14641 gaactctcgc ttgacctaca tctatatatt cctgcaagaa ggtcaattcc tctaacaaac
    14701 gttctttttc cttcttctgc gcttctactt ttgaagttcg agtcgactca gtaaaggtct
    14761 cttccaaggc tctatttttc gctaactgtt cttctaactc ttgcgaacgt ttagaaacat
    14821 ccaaggtggc caaggtgata taggccttgt ccttaaaatt atctaacaat tttttataat
    14881 aggcttcatt cctcaaatga atctcttttc gcttctcagt ttttttcatc aactccgaaa
    14941 atgaatctaa tagttccagt ccaccatatt gaatctcaag ccctttgttt cgtgctgttc
    15001 gaatagcctg ttttgttgac ttggaaagtt tatcttcttc aaaattttcc ttgtatattt
    15061 tcgcctgaat acgaggttga atggtgtctc ccatttcctc cgtttttcct gaccacctta
    15121 ctcccatttg ttgcaaacta tcaataatag ccagattttc aggaaattct gtcttttcct
    15181 gattgattaa actttgagat aggcaaatac ttgggtcaaa agtcacaaaa accgctctct
    15241 tactgcgagc ataggactta atagactgaa tggcaaaatt caagagttct ttatccccat
    15301 aatccaatat aggtcctctt gggatgtaaa acattttata gcctagcgga agagttctaa
    15361 tcaaaatact agctgtcgcc agtaattttt cttccctgta aacaccaaac ttctcatgtt
    15421 gccaattaga cttaacttcc tcccaagcac tactttgtaa tacattggct aattcatgtt
    15481 ctttgacaaa ctgatcatat tctaatgtgg gaatgccaat ttgataacgg tacatttcct
    15541 actctctttc ctccagtatt ttattactat tctactactt tcgtttgaaa agtctagtga
    15601 aaacatattt tattttagtt ctactccccc tatgctgaag ttcaaactaa gtcagtctct
    15661 cttctaattt gaaatcaatt ggtagggttg ttaaaaaaac ttccaattgt ttttcccagt
    15721 agtaccactc gtgagttcca gcgctatggc tataggtcac atcaaaacct agttttttga
    15781 gatttttcac tgcgagatta ttggcttcgt acaagaaatc ctgttcgcca caccacgccc
    15841 aaagtttggt ctttttatcc gattttttag ccagactttc aagagaatag ggactagttg
    15901 tccagtctct aatctctcca aaaacacctc tccagtaggc tggacttccc agattttgac
    15961 tttcaggaga aaagttttga aagctgaggg cacctgaaaa actagctgca tgagaaaaac
    16021 gatttgtcgc aagagccagt ttgaagcagc cgtaacctcc catagaaaga ccagcgataa
    16081 aggtcttttc acgcttgcta gtcatattag ggaagaagcg tttcagaacc tgtggcaatt
    16141 cctctgctag agccgtgtag tagtcaaaac catactgggt atcggtgtac caaccattgc
    16201 tggtattggg cataacaacg atgagattag ttcctcgaag caagcgttct acattggtcc
    16261 gcttaagcca actattatga tttccagaca tcccgtgcaa aaggtacaag acgggaatat
    16321 cttcacactc tggttcttcc actcgattgg catcagggta gaggacattc accccccact
    16381 ccatatccaa tacttgtgag taatactcga ttttcattac tgccatgatt ttctccttct
    16441 atttttctat aagaaaaagc cgaaactcga ctttctctct gtttatttca acgattattt
    16501 tgcttccatg actactggta aaatagctgg acgacgcttg gtttggtcaa agagatactt
    16561 ggtcaaatta tctcgaacct tccctttaag atctgcccag tcaaagtcat ctccttgaag
    16621 ataatcttct accgtttggt taatcaattc tgaactttca cggagaatat cgcgactctt
    16681 cttgagataa acaaatccgc gcgtatgaac acgagcccta gccacaattt tcttctcacg
    16741 acggttgact gtaatagcca cgatgaaaat tccatcctct gacaagatct tacggtcacg
    16801 aagaacaaca tttccaacat caccaatggc attcccatca atcaagatat ctcctgctga
    16861 aaccgatcca gctggaacaa agtctccatt ctcgtaagcc atggtcgtcc cctttttagg
    16921 aatgaagatg tgttctggca acatcccaac tgccatggca gccttagcgt gagcatccaa
    16981 ctcacgatac tccccttgga cagggaagag gtacttaggt tgcaaaagat tgatcatcag
    17041 ctgcaaatca cgcacatttc cgtgccctga tacatgtaaa ctttgggtaa tcaatttgac
    17101 aaccccacct gcctgataaa tcatattttc cacacgcgca acaaaggctt ctttagcaat
    17161 agacggagcc gtagcaatat agaccaggtc cccatccttg atttctacat aacgatggcg
    17221 accaatcgac atcttacgaa gtccattgat aggttcaccc atacgacctg tctcaagaat
    17281 aatcaactca tggtcttcaa agcgagacat atctttaggc ttaatcaaga gaatttcgtt
    17341 ggctaaagac aacttcttaa gacgaatcgc tgtgcggacg atattttcaa tatcaaatcc
    17401 tgtcaagacg atacgtcgac ctgttttatc cgcagcgtca aaaatctgct gaatacgaga
    17461 aagattactg gaaacagctg caacgatgat acgaccttcc cagtcagcaa tagtttgggt
    17521 aatttcatcc ctaacttcac tttcactagc cacctgaata ttgctgtctg cattggccga
    17581 atcactgagg agagccagga cgccgtcacg accaatctct gccaaacgag caaaatcagt
    17641 tgcataagat tcactagccg tttggtcaaa tttgaagtca cctgtataaa cgatgcttcc
    17701 ttccgatgtc ttcaagacaa ttcccagact ctctggaacg gagtaagtcg tagggaagaa
    17761 ggaaaccact gtcccaccaa aatcaatctc cgtattctca tcaatgacat ggaaatcatt
    17821 aaatttctta acggcatcat ttcctttgac aaagagcttt gccaactcaa tggtcaactc
    17881 agacccaaat acaggaactt tagcctctgc caagagataa ggtagagcac caatggcatc
    17941 cgcatgcccg tgggtcaaga aaaccccagc aatacggtcg ctattttcaa aaaggtaatc
    18001 catgtttgga atcaccacat cgacccctaa ttgttcattt tcaggatatt ttaaccctac
    18061 attcaaaaca aaaatggact ctccaatttc agcaatgtac atattttttc cattctcacg
    18121 cacaccacca agtgttgtta aactgatatt actcattttt cctccaaaat cttagtttat
    18181 cttgattata gggcaattct accctcttat tctaaaagtt ctgatacttt cagccgaatc
    18241 ttttcctacc attataccat ttttttgatg attttcaaaa tgaaggtctg ttttcaaaaa
    18301 agagttgatt tcccaactct ttcctaactt ctactctttt tccatcaaaa aatcataaat
    18361 ttcttgcacc gtacccagcg ccatcatttc ttggtcttta acggtttgtt tgagattttg
    18421 gtaaatttga ctttctccta tctcccgctt tggcgcctct ttattgactg tcatcacacc
    18481 atcttttatc gtcacaaagc ctagttcttc aaatacttga atcatcttga ccagcaagat
    18541 ttgttgaata ttaagatatg cagccaaatc tttcagcttg tagcgaatat caaactctgg
    18601 gaactggtaa atagtcttgt acaatttagc aaactgatct ctagtcccat aacctgtcag
    18661 ataataggtc ttgtcaatat catttttgaa atagacagca gagaaatgct gttcctgaaa
    18721 aatggtcttc agctgagtaa tatcctctgg aatgtttttt acgacaacag cttcactagc
    18781 cgcaagattt ggcagttctc caggaaaatc caagactgga acaccttctg gcaagactgc
    18841 attttttcca cgaatgttaa aaagttgaac accttccact cgcgcatcca ccatcatcaa
    18901 ctggagggca gtttggccat tccattggtt gacagacaat ttaaccgcta actctagatt
    18961 cttggtttga gaaaactctg tcgcccatct gccttgacca aaggctacca cttcaaaact
    19021 cgcctcaccc ttggaaattt tcagctttag atgggcatta cctgccccca tagtacgagc
    19081 actttcgacc tgaaaattct tgatataaaa aataggtttc tgattatcca ttccaaaagg
    19141 agctaaacgt tcaaaacttt tgaccgtttc caagctaagt gcctccaaat ccaactcttc
    19201 atctaggttt aacttattct tgccaccagc atctgcacct ttttcacgaa cataatcttc
    19261 caaaacctga gataaatctg agagttgctc aacttccagc gtcatacccg ctgcacctgc
    19321 atgacctcca aaggcgatga agaggtctcg atggggatcc agagcttcaa aaatatcgac
    19381 cgcttccaca ctacgagcac tgcccttggc acgaccgtct tctatattaa gaacaatgac
    19441 tgtctgtccc aattcttcca ataaacgacc agccacgatt cctagaaccc caggattcca
    19501 gccttccttg gccaagacct gaaccttctt ctcaggatcc accatggtct tggcttcttc
    19561 atagatagac tgaacgattt ccttgcgctc ttcgtttttc tggtgaatca taagggcaat
    19621 ctcatgcgct tcctcatcat caaatccagt caacaaatca atggcaggat tgggatcatc
    19681 caagcggccc aaggcattca aacgaggagc aatctggaaa ccaaccgttt cttctgttac
    19741 ttcgttggca gcaatcccag ccatgtccag catttcttgc agaccaatgc gctgggtatg
    19801 acccaacatt tccagaccat attgaactaa gatacgattt tcatccgtca gactcaccat
    19861 atctgcaata gttccaatag cgaccaaatc aagcaattcc acttgcactt cttctaacag
    19921 ggcacaagcc aacttgaaag caactccaca accagccaaa tatttaaaag gataatccgc
    19981 atctggatgt tcaggatgga caatagcata agcatctggc agggtttcag gcatggaatg
    20041 atggtctgtc acaatgacat ctactcccat agactgagcc aatgcaatag cctcatgacc
    20101 agcaacccca ttgtccaccg tcacaatcaa ggaaattcct tcttgctcga taaagtattt
    20161 ataaacacta gcattagggc catagccatc ggtaaaacga tttggcaggt aaactcggca
    20221 ctcagcacca agttgttcca aactttcctt cacaatagaa gccgaagtca tgccatccgc
    20281 atcatagtct ccataaacga gaatattttc cccttcttca atagcctgac gaatccgctc
    20341 cactgccttg tccatatcat ggagcagata agcatcatgt aagtcctcca aggaaggttc
    20401 taaaaacttc ttcagacttt cttggtcctg aatccctctc tcaaacaata accgagccac
    20461 ctcaggaccc agtccagcct tcttggctat ctttgtaaaa tccgcatctt ctacctgcag
    20521 ggcaaactgc cattcataag taggtgttat caaaaagaca tccactcttt ctaagaattc
    20581 tattgcttca ttataacatg atttagacgt tttctctatc cagattgctt ttttataaca
    20641 ttttatagta tattgaaact agaatagtac acctctactt ctaaaatatt gttagaaatc
    20701 gatttgactg tcccgatcga tttgtcctgt tcttatttca ctatagtgaa ttttttcaga
    20761 tatggtttga caagaacatt ctttcattgt agaatcgtgt tataaaatat aacacaaagg
    20821 agatctctca tggctttagt agaatttaaa aacgtcgaaa aatattacgg agactaccac
    20881 gcactccgca acatcaatct ccgttttgaa aaaggacaag ttgttgtcct gcttggacct
    20941 tctggctctg ggaagtccac tcttatccgt acgatcaatg gtttagaggc tgttgacaaa
    21001 ggaagtctcc tagtcaatgg gcaccaagtt gctggtgcca gccagaaaga tttggtacct
    21061 cttcgcaagg aagtcggcat ggtttttcaa cattttaacc tttatccaca caaaacggtg
    21121 ttagaaaacg tgacacttgc gcccattaaa gttctaggaa ttgataaaaa agaagctgaa
    21181 aaaaccgccc aaaaatatct ggaatttgta aatatgtgag acaagaaaga ttcctatccc
    21241 gccatgctat ctggtggaca aaaacagcgg atcgccatcg ctcgtggtct tgctatgcat
    21301 ccggaactcc tcctctttga tgaaccaaca tctgctcttg atcctgagac tatcggagat
    21361 gttctagcag ttatgcagaa actggcgcat gatgggatga acatgatcat cgttacccac
    21421 gaaatgggct ttgctcgaga ggttgcggac cgcattatct ttatggccga cggagaagtt
    21481 ttagtagata cgacagatgt cgataacttt tttgacaatc caagcgaacc tcgtgcccaa
    21541 caattcctca gcaaaattat caaccacgaa agtgacaaag tcaaataagg aggcgcctat
    21601 gaaaaaaaat tctttttatc agcattattg attagccttt tcggccttgc tgctgccaaa
    21661 ccagtccagg ctgatactag tatcgcagac attcaaaaaa gaggcgaact ggttgtcggt
    21721 gtcaaacaag acgttcccaa ttttggttac aaagatccca agaccggtac ttattctggt
    21781 atcgaaaccg acttggccaa gatggtagct gatgaactca aggtcaagat tcgctatgtg
    21841 ccggttacag cacaaacccg cggccccctt ctagacaatg aacaggtcga tatggatatc
    21901 gcgaccttta ccatcacgga cgaacgcaaa aaactctaca actttaccag tccctactac
    21961 acagacgctt ctggattttt agtcaataaa tctgccaaaa tcaaaaagat tgaggaccta
    22021 aacggcaaaa ccatcggagt cgcccaaggt tctatcaccc aacgcctaat tactgaactg
    22081 ggtaaaaaga aaggtctgaa gtttaaattc gtcgaacttg gttcctaccc agaattgatt
    22141 acttcccttc acgctcatcg tatcgatgcc ttttccgttg accgctctat tctgtctggc
    22201 tacactagta aacgcacaga actactagat gatagtttca agccatctga ctacggtatt
    22261 gttaccaaga aatcaaatac agagctcaac gactatcttg ataacttggt tactaaatgg
    22321 agcaaggatg gtagtttgca gaaactttat gaccgttaca agctcaaacc atctagccat
    22381 actgcagatt aaggaggaca ccccatgata gatttatcat cttggacagc ctattttcag
    22441 gattttggac aatttttcaa tggtttcctc tttactcttg ccctagcgat tggatccttt
    22501 atcctcgcca tggtcttagg aatcttcttt ggtgccatgt caaccagtaa acgtccaatt
    22561 ttacgcattt tagctcacat ctttgtggaa ttttaccaaa acactcccct cttggtgcag
    22621 tttgttattg ttttttatgg cctacctctt atcagtgacc atactatcat gattccaatt
    22681 tattggacag ctgttctctg catgggactc tatcacggcg cttatatcgc tgaggttatt
    22741 cgttcaggga ttcagtctat tcctagtggt cagatggagg ccgccttgtc gcaaggtttt
    22801 acctatatca gtgccatgcg cttgattatc ttgcctcaga ccttccgcat cattctccct
    22861 ccattgacca accaaattgt taacctcatc aagaacacct ctactgtagc tatcatctct
    22921 ggagtagact tgatgtttgt gactaagtct tggtcggctc tcaacggaaa ctatatccca
    22981 gcctttttag gtgctgctct tctctacttt gctctatgct tccctgttgc ccagtttggt
    23041 cgcaagatgg agcaagccaa caaaaaagcc tattcacttt aggaggttac tatggaatct
    23101 attttagaag ttttaacccc agataaccta gtctttatct ttaaaggatt tggcttgacc
    23161 ctctatattt ctctgattgc catcatcctc tctactatca tcggtacggt gctagctgtc
    23221 atgagaaatg gcaaaaatcc tgtcttacgc attatttcca gtatttatat cgagtttgtg
    23281 cgcaacgttc ccaaccttct ctggattttt actatctttt tggtgttcaa aatgaaatcc
    23341 acaccagcag gtattacagc ctttactctc tttacatcag cagccttggc tgagattatt
    23401 cgaggcggtc tcaataccgt agacaaggga cagtacgaag caggaatgtc acaaggcttc
    23461 acctcagccc aaatcctcta ctacatcatt ctcccacaag ccatccgcaa aatgctacca
    23521 gccatcattt ctcagtttgt taccgtgatt aaggatacca gtctcctcta ctctgttatc
    23581 gccctacaag aactctttgg agccagccaa attctcatgg gccgttattt cgaaccagag
    23641 caggtcttca gtctttgcat cctgattgcc ctcatctact tcagctttaa cctagcaatt
    23701 tctagcctgt ctcatatgct agccaaacgt tggcaacaag ctgcagaata atactcttcg
    23761 aaaatctctt caaaccacgt cagcttcacc ttgccgtata tat
//
LOCUS       DAVBCV010000029        23482 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBCV010000029 DAVBCV010000000
VERSION     DAVBCV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.029"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(1..319)
                     /locus_tag="VQ516_001157"
                     /pseudo
     CDS             complement(1..319)
                     /locus_tag="VQ516_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(395..796)
                     /locus_tag="VQ516_001158"
     CDS             complement(395..796)
                     /locus_tag="VQ516_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2511606.1"
                     /translation="MDSASIIAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEIANWH
                     IKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTFLSGKSEKQGIT
                     LYHHDQCRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(804..1109)
                     /locus_tag="VQ516_001159"
     CDS             complement(804..1109)
                     /locus_tag="VQ516_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HET2511607.1"
                     /translation="MFPVETEEITYKRKKSKGKRQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
     gene            complement(1096..1290)
                     /locus_tag="VQ516_001160"
     CDS             complement(1096..1290)
                     /locus_tag="VQ516_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2511608.1"
                     /translation="MKIIQQQSATIDSLTNALALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(1366..1706)
                     /gene="tnpB"
                     /locus_tag="VQ516_001161"
                     /pseudo
     CDS             complement(1366..1706)
                     /gene="tnpB"
                     /locus_tag="VQ516_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1687..1872)
                     /locus_tag="VQ516_001162"
     CDS             complement(1687..1872)
                     /locus_tag="VQ516_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511609.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2176..3738)
                     /gene="guaA"
                     /locus_tag="VQ516_001163"
     CDS             complement(2176..3738)
                     /gene="guaA"
                     /locus_tag="VQ516_001163"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2511610.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3880..4578
                     /locus_tag="VQ516_001164"
     CDS             3880..4578
                     /locus_tag="VQ516_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2511611.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4621..5517)
                     /locus_tag="VQ516_001165"
     CDS             complement(4621..5517)
                     /locus_tag="VQ516_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2511612.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLLIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5526..6461)
                     /locus_tag="VQ516_001166"
     CDS             complement(5526..6461)
                     /locus_tag="VQ516_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2511613.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6458..7183)
                     /locus_tag="VQ516_001167"
     CDS             complement(6458..7183)
                     /locus_tag="VQ516_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2511614.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7267..7902)
                     /locus_tag="VQ516_001168"
     CDS             complement(7267..7902)
                     /locus_tag="VQ516_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2511615.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(7904..8731)
                     /locus_tag="VQ516_001169"
     CDS             complement(7904..8731)
                     /locus_tag="VQ516_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2511616.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8753..9089)
                     /locus_tag="VQ516_001170"
                     /pseudo
     CDS             complement(8753..9089)
                     /locus_tag="VQ516_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(9090..9806)
                     /locus_tag="VQ516_001171"
     CDS             complement(9090..9806)
                     /locus_tag="VQ516_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2511617.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSKTVEKDGDKKLIIY"
     gene            10329..10940
                     /locus_tag="VQ516_001172"
     CDS             10329..10940
                     /locus_tag="VQ516_001172"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2511618.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            10985..11713
                     /gene="rlmB"
                     /locus_tag="VQ516_001173"
     CDS             10985..11713
                     /gene="rlmB"
                     /locus_tag="VQ516_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2511619.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(11752..12663)
                     /gene="trxB"
                     /locus_tag="VQ516_001174"
     CDS             complement(11752..12663)
                     /gene="trxB"
                     /locus_tag="VQ516_001174"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET2511620.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12729..12953)
                     /locus_tag="VQ516_001175"
     CDS             complement(12729..12953)
                     /locus_tag="VQ516_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2511621.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13031..13774)
                     /locus_tag="VQ516_001176"
     CDS             complement(13031..13774)
                     /locus_tag="VQ516_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2511622.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILLLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13774..14574)
                     /locus_tag="VQ516_001177"
     CDS             complement(13774..14574)
                     /locus_tag="VQ516_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2511623.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14732..15088)
                     /locus_tag="VQ516_001178"
     CDS             complement(14732..15088)
                     /locus_tag="VQ516_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2511624.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15101..15613)
                     /locus_tag="VQ516_001179"
     CDS             complement(15101..15613)
                     /locus_tag="VQ516_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2511625.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15613..16107)
                     /locus_tag="VQ516_001180"
     CDS             complement(15613..16107)
                     /locus_tag="VQ516_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511626.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16242..16688
                     /locus_tag="VQ516_001181"
     CDS             16242..16688
                     /locus_tag="VQ516_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2511627.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16685..17332
                     /locus_tag="VQ516_001182"
     CDS             16685..17332
                     /locus_tag="VQ516_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2511628.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17353..17934)
                     /gene="pdxT"
                     /locus_tag="VQ516_001183"
     CDS             complement(17353..17934)
                     /gene="pdxT"
                     /locus_tag="VQ516_001183"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2511629.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(17935..18810)
                     /gene="pdxS"
                     /locus_tag="VQ516_001184"
     CDS             complement(17935..18810)
                     /gene="pdxS"
                     /locus_tag="VQ516_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2511630.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(18962..20341)
                     /locus_tag="VQ516_001185"
     CDS             complement(18962..20341)
                     /locus_tag="VQ516_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2511631.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20636..21559
                     /locus_tag="VQ516_001186"
     CDS             20636..21559
                     /locus_tag="VQ516_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2511632.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21619..22224
                     /locus_tag="VQ516_001187"
     CDS             21619..22224
                     /locus_tag="VQ516_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2511633.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLTHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22242..>23482
                     /locus_tag="VQ516_001188"
     CDS             22242..>23482
                     /locus_tag="VQ516_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2511634.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGAS"
BASE COUNT         6972 a         5279 c         4282 g         6949 t
ORIGIN      
        1 atacttgagg ctgtattcaa ttgcccttcc tagttttgaa cccgataaaa ctgactgacg
       61 ccggcaccaa gcaaagaaat cttccattag gttggagctc ttcttgacgt ttctgtagtc
      121 gttcatcagc tggcaaagcc tcccagtctc tttccaagaa aaataactga tcacaataag
      181 ctaaaccttt agctcctaag gatgatttat ccgcttgctt gggggtcgct tcaaaaaact
      241 tccttctcac atgcgcccaa catccaacaa gtgcagcttc ttccagttga cgataagctt
      301 ctagcggttc gcagtttacc aagcttagcg tcgccttgct cctaaacctt ggactgctat
      361 cgcataggca gaactagagg actaaagtcc taagttactg ccgcaacata tcacaatgca
      421 catagccaga ataatctcct aggaattctt gtactactga accacttcga cactgatcat
      481 ggtggtaaag cgtaatccct tgtttctctg atttacctga caaaaaagtc caatagtaag
      541 tcagctgact atcactttct aagacccgat aagaggtttc atctgcatga agaagaggtt
      601 gttctaacaa cttttctcgt aaaaggttat aaagggactc taaatagtat tgactcgcct
      661 tgatatgcca attagcaatt tccttacgtg tgattggtaa acccatccta gcccaatctt
      721 cttcttggcg ataattaggt accttcagat taaacttctg atggatggtg tgagcgataa
      781 tagaagctga gtcaatagtc tcgttataaa ggggctttag gaataggagc tttcacaatt
      841 ttatcactcg gatttttatc actgcatgct tggcatttgt aagcgtgttg gatatgatct
      901 atgcgtttta attgcgcagg aataaagact aattcttgtc gttgaagggt tgctccaatc
      961 tcttttagat ctccctgaca atcagagcaa atgctctctt ctacttgatg atgaacttct
     1021 tctgaatcaa attgggcaag aagagcttgg cgtttccctt tagatttctt acgtttgtag
     1081 gtaatttcct ctgtttcaac tgggaacatc tccatctttc tcagatggag actcttcctc
     1141 aaaaaggttg agttgtccag aggggcaaac acttttctca gaggattttc catagagctt
     1201 ttgagtcaga taagccactt gttcacgaag aagggcaagt gcattggtga gactatcaat
     1261 tgtagcactc tgttgttgaa tgattttcaa tcttcttcca tgggcttccc ctccttgttt
     1321 tctaccttta ttatactaaa aagaaagtcc tcatttcaat agaaatcacg actttctgat
     1381 ggatttattt ttggagtgat agaaaagtcc ttcataagcc agtctacttg ttcagatgtg
     1441 agagctttga catccttttc tgtactgggc caagtcagtc tgccgttccc aaagcgttta
     1501 tatagtagcc aaaatccttg accatcccag taaagggctt taaagcggta tttacgtcca
     1561 ccacaaaaga gaaaaacttg acctgagaaa ggatctaatt caaagtgggt tttaaccaga
     1621 taagccagtg aatcaatgcc ttgcctcata tccgttttcc cacatacgag atagacctgc
     1681 cctaggctag atagatgaat tgtcatagag caacacctta tccaaaagct gttctaccat
     1741 ttcgagattg agagattgaa aaaaacttac ttcaagcttg ccaatacgaa ttttaagcag
     1801 aatatcattt ctctttttag aatcaaaagg acgagattgt ggaatctcta caggaacaat
     1861 aggttgtggc attaaaatat cctccaatag ttttacttta ctaatagtat acaggaggaa
     1921 ctccatgaat gatagatacc ttgttatgac gcgattacta tattagaaag ttggctctct
     1981 aattaaatta attctcatta tatatatcaa aagaccgcag cttgaaaact acggtctaat
     2041 tttttgtcca aatgtaaacc acttggaact attattcaat ttattctttt tagtaaatgt
     2101 tcccgtttta atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt
     2161 ttaatttttt tgcgattatt cccactcaac tgttgcaggt ggtttacttg taatatcgta
     2221 gacgatacgg ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac
     2281 ttcccatgga attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat
     2341 tgcaatcgtg tagtcatatg tacgaccgtc acccataaca ccgactgaac gaacgcctgt
     2401 gttaacagtg aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg
     2461 aagaatagcg tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac
     2521 acggatagca agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc
     2581 aagctctgta ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa
     2641 ttcaaactgc atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc
     2701 tgtatccgta ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac
     2761 atctttgagc ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt
     2821 acgtttttgt tcaggatcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc
     2881 tgctttgacg atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc
     2941 gcctttacga agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt
     3001 ttggagaaga accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg
     3061 tttatcaccg acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga
     3121 ccagtcacct ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta
     3181 tacagaatga cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc
     3241 gatggctgcg tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc
     3301 agtaaccgca tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag
     3361 cgctgatggt gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc
     3421 aggaacaact tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa
     3481 aattggaatt ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac
     3541 agaatttgga ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc
     3601 tgaaatttta tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat
     3661 cagctggttg tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc
     3721 agttgaaatg ttgctcatct ttttcctttc tcaaaagaaa tatcttttct caatattcta
     3781 tcacaaataa gggcgaatta ccaactaaac aaggcgaagt ttgacttttt ctagtttatt
     3841 ggggtacaat agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa
     3901 atccatgatc agataaaaaa ggatattgac gagcaccgtt gggctattgg tgagaggctt
     3961 cccagtgaaa gagatttagc tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc
     4021 gtatctctat tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt
     4081 tccagtactc gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa
     4141 tcccaaggta aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag
     4201 caagaagttg ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc
     4261 cgctatgccg accaagttcc cctagtttat gaagttgctt ctattcctga aaaattcatt
     4321 aaggacttta aaaaagaaga aatcacaagt catttcttcc aaaccttgca aaaacatggc
     4381 tatcgtatcg gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc
     4441 cactatttgg aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta
     4501 gaagatggta ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt
     4561 tatcttgaaa ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt
     4621 ttatttttct aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc
     4681 cataccaaga aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaat
     4741 cgccgatatt ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc
     4801 tcctttaagg aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc
     4861 aaggagaaaa tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc
     4921 gaccaaacct gctatgacca ttcccacacc aatgagcaag ctgctccccc actttttaat
     4981 caaggctatg ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa
     5041 cagaccagca ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt
     5101 tcctccgatg gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt
     5161 gatacagcta taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc
     5221 cgcattggtc tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag
     5281 agcaaaaata agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc
     5341 ctttgcagta gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt
     5401 ggtcaagacc agagccgaaa ttccgtgtgc cattaggtat tggccactcg ttcctgacaa
     5461 cccccaagca ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc
     5521 tctccttact cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga
     5581 gctctctcgt ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga
     5641 cgattccaca tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa
     5701 ttccatgcta aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt
     5761 gcaaaatcat ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat
     5821 aaaccagcac tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct
     5881 gcctctacac ctctaactgt tggaacagca agctcaactg gcccaaactt ggtttccgtc
     5941 attccccaag gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt
     6001 ctttccaaaa taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga
     6061 cttcgtctgt tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga
     6121 ttaggaataa aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct
     6181 ggataatcac tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt
     6241 tcccacaaga aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctagatca
     6301 taaactagtc ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac
     6361 tctgtccatt gattgtcctt aaaatacgca aaagaggctc cgggataaat tcctgcctcg
     6421 atttgttctt ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt
     6481 caacattatt tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag
     6541 tagactcaaa cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca
     6601 tagataagtc ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa
     6661 aatcaaggga tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat
     6721 gtaattccat agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt
     6781 caggcttttc tcctacttct actagacttg ctctgtcatc ttccgcatga atcaaaatca
     6841 aatcatcttc tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat
     6901 gaatcgaatc tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa
     6961 tcaatctagc tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt
     7021 ctaaaaccag cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta
     7081 acaaggcctt cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag
     7141 cttttaaagt aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt
     7201 tttattttaa aggattttag aattttttac aagatattat gctcaaattt aacattattt
     7261 agctatttag taaagatagt ctttcaagga ttttgacaaa gcctgataac cagcaatact
     7321 gaggtgcagt ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca
     7381 atcaaatact ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg
     7441 attccagttc tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctcc
     7501 ctcattgaca ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac
     7561 ggattgaatg atagcttcga gattattgag agcctcattc acaggaacat cctttccgat
     7621 atcatttgtc ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc
     7681 aagattctct agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcgtctt
     7741 tgaagtccca aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag
     7801 aatgtttggt tctacaacag aaatgtgatt taggtgacga tacttagttt gaattttttc
     7861 ttgttccttt aggagccaat tttctaataa ctgtactgcc acttcattct cctttttcta
     7921 accagttttc caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg
     7981 ccacagcttt ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa
     8041 gcatttcaac atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc
     8101 agcgcttgag taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa
     8161 tgcaaccata gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt
     8221 ccaaaaccga actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa
     8281 agaggtcaga tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt
     8341 ctggagtcat aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct
     8401 tcatccccgt tacaacaaac tggtcctgac acgctcgacc cttgaaatga gccaaagcct
     8461 tgttgacaat ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac
     8521 gagcgccatt agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca
     8581 tgcggtgaat ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat
     8641 ctaaaatctt ttccaatcgt gggagatcaa gctgacccct agcatccagc aaggtcccat
     8701 ccatatctgt cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc
     8761 acttcaatcc cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg
     8821 cttcatctaa ctggtcacga tgactgccac ccctctataa aatcttccaa aatataagtt
     8881 tggtaatgat acaagggata tccttctggt gaaaaggttc cctttggtgt tcgattgacc
     8941 caagtaggat gagctttagc tgttccgata gttgtttttc catccttctt cttgatggtg
     9001 acatccatga gaacaccacg ttcagtccac ttagcattct cttcatctcc catagattca
     9061 atccgttgat tggaaatgaa gttccccatt taatagataa tgagtttctt atctccatct
     9121 ttttcaaccg ttttagatgg ttcaacaacg tgaggatgcc ctccaaagat aatatccgct
     9181 ccccaatcga tcatcttgtg ataaagagct ttttgttctt cagttggttc caatcgatac
     9241 tcaacaccca tctgaggcat gataatggtg atatctgctt ccttctctgc ccgttcgact
     9301 tcagccttta tcttatcttc gtttaaatct gaaagataac gattatagtc ttcctgaaaa
     9361 atatactgct caattccatt gaaaccatag gaataggcta acaatgcaac cttgatagca
     9421 ttcacttcct taatgaccag cggagcctga tcacgtggtt cgtgcgtata aactccgatt
     9481 ggagtgattc cagctttctc aataatatcg gccattgaaa taactccctc aatttgcgaa
     9541 tccaaaatat gattatgagc taaatccagc acatgataac ctgcttcctt aatagcatcc
     9601 ataacttcaa caggagcatt aaagagaaga taacccgcta aataatgatc cttattaatg
     9661 gttccttcaa aatcaccaat agctaaatct gcttgcttga gccaaggagt cacatactca
     9721 aaattttcat gaaagtcata ggtaccgtct tcttttttag ctgaaaagaa aagtccatcg
     9781 tggtagagca aatccccgtg ggccataatt ctggcagttt tttcctcttc ctactcctga
     9841 gacttttgct tagtcccctc ttgagaaata gtatctcgtt tttgactagt caagggattc
     9901 cttggaagct ttcaaacaac aagaccaagc ccattgataa accaactgct agcaagagta
     9961 tcgccacaaa tcccttattg ctccacttgc gataactcct aaaaagttta ccaagccctt
    10021 cataaaacga aaagctaaac caccctgatt tcgattttgt cttctttgta tcttcattct
    10081 ccctactttc ttatgcaagc cttttctttt tattatatca cagataagta tttctttcac
    10141 aattgaattg aacttcccat ctattttcta taaatcctaa atgccataat gctttcaatt
    10201 cttgtcattt tgtgatatca tgtagaggta atgaaaggaa atgaactaat ccacagtggc
    10261 ttattccaag tataccactt gggctttggc agtagttaac tgcactaaac ataatataag
    10321 gagagaaaat gtctgcaata gaacgtatta caaaagctgc tcacttaatt gatatgaacg
    10381 atattatccg tgaagggaat cctactctac gcgcgattgc tgaggaagtc actttccccc
    10441 tatctgacca ggaaatcatc ctaggcgaaa agatgatgca attccttaaa cattcccaag
    10501 atcctgtcat ggctgaaaaa atgggactcc gcggtggtgt tggactggct gctccccagt
    10561 tagatatctc aaaacgcatt atcgctgttt tggtacctaa tattgttgaa gaaggcgaaa
    10621 ctccacagga agcctacgat ttggaaggca ttatgtacaa tccaaaaatc gtctctcact
    10681 ctgttcaaga tgctgctctt ggcgaaggag aaggttgcct gtctgttgac cgtaacgtgc
    10741 ctggctatgt tgttcgccat gcccgcgtta ctgttgacta ctttgacaaa gatggagaaa
    10801 aacaccgtat caaactcaaa ggctacaact ccattgttgt tcagcatgaa attgaccaca
    10861 ttaacggtat catgttttac gatcgcatca atgaaaaaga cccatttgca gttaaagatg
    10921 gtttactgat tcttgaataa agaaaatccc gttgcaagac ggggttttgt gttataatag
    10981 aggcatgaaa acaaatgata ttgtctatgg tgtccacgcc gttaccgaag ccctccttgc
    11041 aaatacagga aacaaactct acctccaaga agatctccga ggtaagaatg ttgagaaagt
    11101 caaggaacta gctacagaaa agaaggtgtc catttcttgg acatcaaaaa aatctctctc
    11161 tgagatgact gaaggtgctg ttcatcaagg ttttgttcta cgagtgtctg aatttgccta
    11221 tagcgagcta gattacatcc ttgcaaaaac acgccaagaa gaaaatccac ttctattgat
    11281 tctagatggt ctaaccgatc cccataatct gggttctatc ttgcgaacag ccgatgcgac
    11341 caatgtttca ggtgtcatca ttcccaagca ccgtgctgtc ggagtaactc ctgtcgttgc
    11401 caaaacagcc acaggtgcta ttgaacacgt tccaattgcc cgagtgacaa acctcagtca
    11461 aaccttagat aaactcaagg atgaaggctt ctggaccttt ggaacggata tgaacggtac
    11521 tccttgccac aagtggaata caaaagggaa aatcgccctc atcatcggaa atgaaggaaa
    11581 aggcatctct agcaacatca aaaaacaggt cgatgaaatg attaccattc cgatgaatgg
    11641 acatgttcaa agcctcaatg ccagtgttgc tgcagccatt ctcatgtacg aagtttttct
    11701 aaatagacta taaaaaagtt tccagtcatc tgattggaaa cttttttatg attaactatg
    11761 ttctgtgata aacttgtagg cttcttgacc agcgatagct ccatctccaa ctgctgttgt
    11821 tacttggcga aggtctttca agcgaacatc tccaactgca aagataccgt cgactgcagt
    11881 cttcatgtgg ctatctgtca caatccagcc tgcctgatct tggatattca attctttaac
    11941 aaaatcgcta agagggtcca aaccaacata gataaagaca ccaccgaagg cttgctctgt
    12001 cacttgacct gttttcacat tttcaaaaac gacagattct actctgtttt cacccttgat
    12061 ttccttcact acagaatccc agataaagct gattttttca ttcgcaaagg cgcgatcttg
    12121 taaaaccttt tgggcacgaa gttggtcacg acggtgaaca atggtaacag tcttagcaaa
    12181 acgagtcaag aagagggctt cttcaacagc tgaatctcca ccaccaacta ccaataaatc
    12241 ttggtcacgg aagaaagcac catcacacac agcacagtaa gaaacaccac gactgttcag
    12301 ttcttcttct ccaggcactc ccaaaggacg gtgtttagaa ccagttgcta cgataactgt
    12361 acgtgtttca tatgtttggt catcagtcat cactttctta aaatcaccat ggtcttcgac
    12421 attttcaaca taaccataaa tgtgctcaac accaagattt tcaagtggtt caaacatctt
    12481 ttcagccaat tcaggtccac taatattagc gtatcctggg taattttcga tatcagatgt
    12541 attattcatc tgaccacctg gcagaccacc ttcaatcaaa gctactttta gattgcttcg
    12601 agcagcatac aaggccgcag tcatccctgc aggtccagca ccgataataa tagtatcgta
    12661 catatagatt ccttctttct tgttgtaact atctttattc taactctttc ttgtcaatca
    12721 cgcaaggatt atgccttgaa aacaagaatt aaactcataa ccgcaaagga taatactggg
    12781 acaaccaaga ctccaatcat aatcatatca tagagatggg cttggcgagc cctggctgtc
    12841 ttatcaactc ccgacatggc tctcagacca atccaaatcc ctaaaaaaat cagaacaagg
    12901 atggtggtca agatcaaact ctcgaaatat aaagaaaata gttgcagtag catgatttct
    12961 ctcatttcta tcttttttaa agagtaaact cagctagtcc aactaactga gttttccttt
    13021 atctattata tcaaatataa gtccgtttgt aactagcgaa gaattctttt gtccgctctt
    13081 ctttaggggt gtggataatc tcatccggag ttccagactc gatgattttc cccttatcta
    13141 ataagagaat tttatccgca acttgggcta caaaggacat gtcatgactg accaaaatca
    13201 tggtctgacc tgacttagca gcatctgcaa tagacttttc tacttcaccg accaattctg
    13261 ggtcaagggc tgaagttggt tcgtctaaga gcaaaacatc tggtttcata gcaagcgcac
    13321 gcgctagggc aacccgttgc ttctgtccac ctgataaatg gcgaggataa tggttttcac
    13381 ggtccgaaag cccaacctta gccaactctt ccttggcaat cttagtcgct tcttggtcag
    13441 ataatttctt gacaacaacc aagccttctt tcacattatc aagtgctgtt cgacgttcaa
    13501 ataaattaaa ctgttggaaa accatagaca acttacggcg tagggcaagg atttcttctt
    13561 gagtgatttt agaaaaatca actgaaaaac catcaatctg aatagagcca ctgtcaggtg
    13621 tttcaagata attgagactg cgaagaaagg ttgattttcc agctcctgaa gaaccaatca
    13681 aggctacaac ttcccctttt tgaatatcca agttcagatg atccaagaca gtctgtcctg
    13741 aaaaggattt gcttaaattc gaaatcttaa tcattaacga aggtctcctt tcacatctgt
    13801 ttgcactgta tcaggtgcag aaatagccat ttttctctcg atgaaacgac cgaggctttc
    13861 aattccgata ttgactaccc aataaacaag ggcaacagag atgaagcgtt caaaatagcg
    13921 ataatcagct ccacctagaa tctgagcttg ggcaaagact tccacaacac ccgcactaaa
    13981 agctagagat gttcccttgg tcaaaccgat gagggaatta atcaaggttg gagtagctac
    14041 caccgctgca ttaggaataa tcactcgtcg ataaacttgc gctcgggtca tacccagact
    14101 gcgcgccgcc tcaatctcac caggattaac tgagagaatg gctgcacgaa tggtttcact
    14161 agcataagct gcctcattaa aggcaaaagc gacaatcgca aaagctgcag ctggaatcgc
    14221 attgatattg agaccagttc cccattgctg attgagggct ttcaaagcca aagggattcc
    14281 gtagtaggtc aacatgagtt gcaccaaaat cggtgtccct tttaagaaac taacaaagaa
    14341 ggcctgcaag ggatataaaa tcttgacacg attgatcttc acaatggcaa aaagaagcgc
    14401 caaaaccaag ccaaaaaggg caccaccaat tgtcaacata attgttgttg gaagttgttg
    14461 gacaattcta gggattccat caaagaccga acgtaggcta aacagcttgc catccggaat
    14521 caattgcatc aagttttggt accaatctga tgctaaaatc gttgtaacat tcataaaaac
    14581 atcctcttct gataatgatt cattctacca tgcttctgcc gtcaatgaaa ttatttgcta
    14641 cggtcagata cagactttgt ctacctacta gtttatcata ctttgaaaca gggaagttat
    14701 atattttatc tatcaaagag atagataaaa actatttcag tcccagttct tcataagatt
    14761 ttcgataacc gatttgctta acagttccat tttctactaa aatgggccgt tttaacaaca
    14821 taccgtcact tgctagcaac tcagccgctt cttggtttga caaacttcct accttatctt
    14881 ttagccctaa ttcacggtat ttgataccac tggtgttgaa aaattgcttc aattcaaatc
    14941 ctgaggtttc tagccaattc aaaatgactt cttggctagg tgtttcttcc acgatatgga
    15001 cggctttata gtccacacct aattgattta attcttgttt tgctttttta caagttgaac
    15061 atttggggta ttcgataaat tctaacatgt gcttctcctt ctatttgttt atatctttaa
    15121 aatctacgcc ttcatgctcc aagagccaag ctttcttttc cactcctgca gcatagcctg
    15181 tcagacgctt gcctgctccc aacacacgat gacaaggtac taggatagac caaggattgc
    15241 gtcccactgc tccaccaatt gcttgagcag aagccacttg caggtcttga gcaatttgtc
    15301 cataggtcac tgtctgacca taaggaatgc cctgtaaata gtcccaaact ctcttttcaa
    15361 aattcgttcc gattggcgcc aagagcaagt tggataaatc ctgaggcttg cctttaaagt
    15421 aatcatctaa gcaagcaata actgggtcta aaataggatg actaacaact tcttctatcg
    15481 tttcatctcc tagtcccctc tcaaaatgct tctgctcctg aacccaaatt ccatacaaat
    15541 aatggtcatc agctacaagt gatagaatac caattggtga ggagtagagt atttttacat
    15601 acttcttttg cattatttct ttaatttttg ataggccaag cgttcgccat ctactggcac
    15661 cttaccaact tgtttaaaac caagtttttc aaaaatatgt tgcataacct tgttttcagc
    15721 atgcgtatct gagcgaaaat caagataatc aaaaccttca atcaagccct ctaagaaggt
    15781 ttgagcaact ccttttccct gcacatctgc tgccacagca atacggtgaa agactagata
    15841 ctctgactct ccagcttgcc agtttccctc ataaatagct tcataggcct cctctggact
    15901 cttggtcaca gcagcatagg ctagcagttc tccctcttcc aaggctacgt aggcttgccc
    15961 tgagatgata tcatcgataa taacatctgc atttggatag ccattttgcc actggtcact
    16021 accagcatct gctaaacatt ttttggcatc ctccatcacc tgcatgatgg catctacttc
    16081 atttggaaaa gctaaacgaa tttccatctt ttttcctcat ttctgactag tttctcccat
    16141 catagcataa tttaagtatt tctacaagca gttaaaactt gactttgtgt ttttgttatt
    16201 ttataatcta gttatcaaaa ctatataaaa aggagttgaa aatgaaaact accttttcct
    16261 acccaaaatg ggcagaaatt ccaaacattg acctctatct ggaccaggtt ttgctctatg
    16321 tcaatcaggt ctgcgcccct atctctccta ataaagacaa gggcctaaca gcatctatgg
    16381 tcaacaatta tgtgaaaaat ggttacctga caaagcctga caaaaaaaaa taccaacgcc
    16441 aacagattgc ccgtttgatt gctatcacaa ccctcaagtc tgtattttct attcaagaaa
    16501 tagcccagac acttaatact ctacaaactc aagcaagttc agaccaactc tacgatgctt
    16561 ttgtggacta catgaaccaa ggaattgacc cagctaaccc tattatccaa accagctgcc
    16621 aaaccgtcaa actctatcat caaactctag acttaatcga tcatactcaa gaggaggtaa
    16681 tccaatgaac actagtctta aactcagcaa acaactcagt tttggagagg agattgctaa
    16741 tagcgtgacc catgctgtgg gtgcagtcat catgcttatc ttgctgccta tttcatccat
    16801 ctatagttat gaagcacacg gatttttatc ctctatcggc gtttccattt tcgtcatcag
    16861 tctctttctc atgttcctat catccaccat ttatcactct atggcctatg gttcgaccca
    16921 caaatatgtt ttgcgaatca ttgaccattc tatgatttac gttgccattg ccggctcata
    16981 cacgcccgtt gtcttgacct tgatgaataa ctggtttggc tatctgatta ttgtcatcca
    17041 atggggaacg accatctttg gtattctcta taaaatcttt gctaaaaagg tcaatgagaa
    17101 atttagcctt gctctttacc tgattatggg ctggttggtt ctggctatca ttcctgccat
    17161 tatcagtcaa acgacacccg ttttctggag tctcatggta actggcggac tctgttatac
    17221 agttggagct ggattttatg ccaagaaaaa accttatttc cacatgattt ggcatctctt
    17281 tatcctagct gcgtccgcac ttcaatacat cgctattgtt tattacatgt aaaaaagttg
    17341 agaaattcaa tctcaacttt tttctttaca catattgata aagtactggt gcaagcgcac
    17401 atcatcagtc aattctggat gaaaagaact taccaacata tttttttctt gggctgcaac
    17461 aatttgattg ttcactgttg ctagaatttc tacaccctca ccaacactac tgataatcgg
    17521 accacggata aaggtcattg gaatcttgcc aactccctta cattctgctt ccgtgtagaa
    17581 acttcctaat tggcgcccat aagcattacg ctcgaccacc atatccatag ttcctagatg
    17641 actctctttc tgagaagtga tttccttagc cagcaaaatt aagcccgcac aggtcccaaa
    17701 cactggtaag ccagatagaa tggcttctcg gatgggaagt agcatgttct ggtcacgtaa
    17761 gagcttgccc atggttgtag actcaccacc aggcaaaatc aaacccgaca agtcactctg
    17821 atcttgctga aaatcatcta gatttctgag ttctacactc tcgacaccta attgatctag
    17881 cacttttgca tgttctgcaa aggccccttg caaggccaat attccaattt tcatctattt
    17941 tcctcgttca gccatgagaa tttggatttc attttcatta ataccaacca tggcttctcc
    18001 taaatcttca gagatttgag ctaggatttg aggattacgg aagttagtca cagccttaac
    18061 aatggcactc gctcgtttaa caggatctcc tgacttgaaa atacctgaac cgacaaagac
    18121 cccctctgcc cctaattgca tcattaacgc agcatctgct ggcgttgcaa cacctccagc
    18181 agcgaaattt acaactggca attttccatg ttcatgaaca tattggacca attctacagg
    18241 gacttgcaaa tccttggcag caacataaag ctcgtcctca cgtaagtttt gaatgcggcg
    18301 aatttcctga ttcatcatac gcatatgacg aacagcttgg acgatatccc ctgtccctgg
    18361 ttctcctttg gtacgaatca tggaagcacc ttcagcgata cgacgcaagg cttcacccaa
    18421 atccttagca ccacagacaa aaggaacttg gaattctttc ttgtccacat ggaaacggtc
    18481 atcagctgga gatagaactt cactctcgtc gatataatca atttcaatag cctctaaaat
    18541 ctgagcttca acaaaatgcc cgattctgac cttagccatt actggaatac taaccgcttc
    18601 ttggatttcc ttaatcatct ttgggtcgct catgcgggaa actcctccag ctgcacgaat
    18661 atcagccgga attcgttcca aggccatcac agctgccgca ccagcagctt ctgcgatacg
    18721 agcctgttca ggattctgca catccataat aacaccaccc ttgagcatct gtgccaagtt
    18781 tttatttagt tcataacgat tttcagtcat ttgttttatc ctcatcattt gtattggtag
    18841 ctaccttttc attttaagtc agaacagaat gaagcacaag ataaaaaaac tataattgta
    18901 tggggacaga ttggctaaaa actatctgac cttaacttaa ggccagatag ctcattcatt
    18961 tttatttttc agccgtaagg gcagccattg tgatgtagtt gtatggttta ttgaagtgtg
    19021 gcaagaagaa gaggtctgtc aatgccaatt tatcaattgt cacatgctct tggatagcaa
    19081 gtgagaacat gtggattccc atgctaattg caatatcatg tgaaaccatt tgggcaccaa
    19141 gaatttcacg gctatctttg tcaaagacaa tcttaattgc tacttcatgg ttgtcatgtt
    19201 tcatgaattc tggtttttga agatcgttaa agcctgtttc agttgcgttg taaccagcag
    19261 ctttcgcttt ttcaagagtc aaaccagttg aaaccatgtg aagaccgtag attgagatac
    19321 catttgaacc ttgaacaccg attccttcca attcatgtcc acaagcgttg taggcaccaa
    19381 cgataccagt gcgaacagca tttgaagcaa gagcgatata gcttgtatct ttacgagcat
    19441 tgtcataaac agtcgcacag tcaccaacag cgtaaacgcc tgggatagat gtttcttgtt
    19501 tcttgtctac aaggaaggca ccgttgcgga agagttcgat cttaccatca gcaagggctg
    19561 tgtttggacg gaaaccaact gcaaggataa ccatatccac gtcaaagctt tctttgtcag
    19621 taatcaagcg ttcaacttta ccatcacctt cgattgcttt aacagtttga cctagagcca
    19681 agcggatgtt gtgatcttcc aagttcttcg ccatcatttg tgtgaagtct ttgtcatagt
    19741 aaccgttcaa gacagtatca acgatatcaa caaggacaac ttcttttcca agacgttcaa
    19801 aggcttcagc aagttcaaca ccgatgtaac caccaccaac aacggcgata cggtcgagat
    19861 gttggctctt gtcagaaagt ttattgataa cttcttcagc attttggtac aatttcacga
    19921 attgtacgtt ttcaagagtt gctttaaatt cgcggtttcc tttaacaatt tcaacacctt
    19981 cgattggtgg caagattggt gtagaacctg tagcgaaaat caatttctcg tatgattctt
    20041 tgtgttcttt tccttcaact tctgctgtaa ccactttgtt atcatagtcg attgaaagaa
    20101 ctggtgaatt catataaacc ttagcacctt tagcttctaa tttgtcttta tcagaataga
    20161 acaagccttc agcaccgtca atttgttcac caatccaaag agccattcca catcctagga
    20221 aagagatgtt agagttttgg tcaaatacaa caatttcgtt ctcatttcca aaattatcca
    20281 acatggtatt gatacatgct gtaccagcgt ggttagcacc gactacaacg attttactca
    20341 taaaaaattt cctgccttca ttctaaattt actttactag tataccattt accgttagcg
    20401 tttacaaggg ggggactatt atttcagcat tttttatcaa aaataattcc ccatttatcc
    20461 tgttttcaag cctcatgaat tttatgagca tatttcttga ctttttccaa attttgtttg
    20521 tgaaaattct aactttatgg tattataata acatgaaaat aaaatgtaag ggcttcaatt
    20581 tagatgatta tttgatattg caacttattt tctataagag aaaggagttg gtagcttgac
    20641 tcttcgttca cattctgaac ggctaatggg gactactatc actatttcat tagtggatga
    20701 gcaagccgat atctttctcc aaaaatcctt tgacttgctc aaagagcttg aataccgctt
    20761 caacgccaat agtcaagaat ctgagttgat ggaaatcaat tatcaagcag gaatatcccc
    20821 cgtcacagtt catccagacc tgtttgagct gatttcactt gggttagagc atagcctagc
    20881 gctctctagc cacctcaata tcagcattgg tcccttaatt caaacctggc gtatcggttt
    20941 ttcagatgcc aaggtcgccc agcctcaaga aattgaatcg gtgctgcctc taatcaatcc
    21001 tcatggtatc gagttagatt cttctacttc tactgtgttt ttaaaacaga aaggaatgaa
    21061 gatcgatcta ggttgtttag ccaaaggata cagtgcggat aaggttgccc aatttcttag
    21121 gaaagagggg gtgacttctg ccttgatcaa tctgggaggg aatatcctga ccattggaaa
    21181 aaatcaggca agaggggata acccatggca aatcgggatt caagacccag ccaatcctag
    21241 gggaaatcat ttaatgacca tccctgttgt caataaatct gtcgtgactt caggcattta
    21301 tgaacgtcac ctgaccgtcg atggacaaga ttaccatcac atttttgaca gtcaaacagg
    21361 atatcctgtt gaaacggaac tagcgagtct aacaatcatc tctgataaat cagtcgatgg
    21421 cgaaatctgg acgactcgac tctttggaga aagacctgct tctatcctct ggcaagtcga
    21481 aagtttggag ggcatcgaag tcatcctcat cgataaagaa ggccacctaa gctgttcttc
    21541 aggaattcct actctataga gacaactgtt tcaatggaat tttaaaattg tattatgtaa
    21601 aaagagaaag gaaatatcat gttaaaactt attgctattg ttggaacaaa ttcaaaacgt
    21661 tctacaaacc gtcaattgct tcaatacatg caaaaacact ttgctgacaa agctgaaatt
    21721 gaacttgttg aaatcaagac cattcctgtc ttcaacaaac cagctgacaa gcaagtacct
    21781 gctgaaatat tggaaattgc tgctaaaatc gaagaggcag atggcgttat tatcggtact
    21841 cctgagtatg atcactctat tccagctgtt ttgatgagcg ctcttgcttg gttgtcttat
    21901 ggtatttacc cacttttgaa caaaccaatc atgattacag gtgcttctta cggtacgctt
    21961 ggttcatctc gtgcccaatt gcaacttcgt caaatcttga atgctcctga aatcaaggca
    22021 aatgttcttc cagatgaatt cttgctcaca cactctcttc aagcatttaa cccaagtggc
    22081 gacttggttg accttgatgt tatcaagaaa ttggatgcca tctttgatga cttccgtatc
    22141 ttcgtgaaga ttactgagaa attgcgcaat gcgcaagaat tgcttcgcaa agatgctgaa
    22201 gactttgact gggaaaattt gtaagatagg agaccgaaag aatgaaattt gttggacttg
    22261 ttggatcaaa ctacgatcaa tcatataacc gcaaactctt ggaatttatc cgtcgcaatt
    22321 tcaaattcaa atttgaatta gaagtacttg aaatcgacga agttccaatg tttaaccaag
    22381 acgaaaaatg ggacgaaagc ttccaattgc gtttcttgta taacaagatt acacgtgctg
    22441 atggtgtcat tatcgccact cctgagcaca accacactat ctcagcttca ctcaaatctg
    22501 tacttgaatg gctttcatac gaagttcatc catttgaaaa caagcctgtt atgattgtgg
    22561 gtgcatcata ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt aaaatccttg
    22621 atgctccagg tgttaatgcc tacacgcttc caggtaacga attccttctt ggtaaagcta
    22681 aggaagcttt tgataacaac ggaaatatca ccaacgaagg cactgttaaa ttccttgaaa
    22741 cttgcttaga taactttgtt aaatacgtag gagtcgtttc gaaattgaaa aaaccaaaac
    22801 caatcgaatc agaagacttg gattgtggaa aaccaatcgc tacaaccatc acagaagttg
    22861 accctgacga tccagaatgg gtagaaaaag ttgcagcaat cactggtgct gtttctggtg
    22921 atacctatgt caaattggac cacggtatcc taacagttaa ccaaattgat atgttcttga
    22981 aagctatgcc atttgaattg acatacgctg acgacaacaa ccaattcctc tactacaaca
    23041 acgctcacca agatccagac actatgttcg ctaaacgtgt accacctcaa tcaggtagcc
    23101 gtatgtcgac tgttcatggt tctcttccac cagcacgtat gaagaacgta gagtgggtta
    23161 tcggaacact tcgcaacgga aaccaagaat acgtccgtac gatcgttcca ggttctcctg
    23221 caggtgttat caacactcac aactaccaag caatgtacta tcctgatgga tcatacgctg
    23281 gtatcaatga aatcgtcttt aacttccaac catggcttga ctggtaccta aaagagactg
    23341 gtcaacgttt ggtgggtggt agcggaccat ttgctcctgc tgccggaggt catggagacg
    23401 ccgatgctac ttctggcgct tctgattcag gggatgctgg aggccacggt ggtgacgcag
    23461 acgctacagc cggtgcaagt ta
//
LOCUS       DAVBCV010000030        23189 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBCV010000030 DAVBCV010000000
VERSION     DAVBCV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.030"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(73..531)
                     /locus_tag="VQ516_001189"
     CDS             complement(73..531)
                     /locus_tag="VQ516_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2511635.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(528..968)
                     /locus_tag="VQ516_001190"
     CDS             complement(528..968)
                     /locus_tag="VQ516_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2511636.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1552..2418
                     /locus_tag="VQ516_001191"
     CDS             1552..2418
                     /locus_tag="VQ516_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2511637.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            2755..3312
                     /locus_tag="VQ516_001192"
     CDS             2755..3312
                     /locus_tag="VQ516_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HET2511638.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            3533..4639
                     /locus_tag="VQ516_001193"
     CDS             3533..4639
                     /locus_tag="VQ516_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET2511639.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            4615..6006
                     /locus_tag="VQ516_001194"
     CDS             4615..6006
                     /locus_tag="VQ516_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511640.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            6003..7181
                     /locus_tag="VQ516_001195"
     CDS             6003..7181
                     /locus_tag="VQ516_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2511641.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            7606..7839
                     /locus_tag="VQ516_001196"
     CDS             7606..7839
                     /locus_tag="VQ516_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2511642.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            8122..10071
                     /gene="mutL"
                     /locus_tag="VQ516_001197"
     CDS             8122..10071
                     /gene="mutL"
                     /locus_tag="VQ516_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2511643.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(10396..10863)
                     /locus_tag="VQ516_001198"
     CDS             complement(10396..10863)
                     /locus_tag="VQ516_001198"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2511644.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(10864..12069)
                     /locus_tag="VQ516_001199"
     CDS             complement(10864..12069)
                     /locus_tag="VQ516_001199"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2511645.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(12089..12724)
                     /gene="ribE"
                     /locus_tag="VQ516_001200"
     CDS             complement(12089..12724)
                     /gene="ribE"
                     /locus_tag="VQ516_001200"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2511646.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(12709..13809)
                     /gene="ribD"
                     /locus_tag="VQ516_001201"
     CDS             complement(12709..13809)
                     /gene="ribD"
                     /locus_tag="VQ516_001201"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2511647.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(13944..14058)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            14215..14808
                     /gene="ruvA"
                     /locus_tag="VQ516_001202"
     CDS             14215..14808
                     /gene="ruvA"
                     /locus_tag="VQ516_001202"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2511648.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            14818..15381
                     /locus_tag="VQ516_001203"
     CDS             14818..15381
                     /locus_tag="VQ516_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2511649.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            15378..16055
                     /locus_tag="VQ516_001204"
     CDS             15378..16055
                     /locus_tag="VQ516_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2511650.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     VGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(16196..17227)
                     /locus_tag="VQ516_001205"
     CDS             complement(16196..17227)
                     /locus_tag="VQ516_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2511651.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRALFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNAGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(17482..18165)
                     /locus_tag="VQ516_001206"
     CDS             complement(17482..18165)
                     /locus_tag="VQ516_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2511652.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASVFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(18177..19121)
                     /locus_tag="VQ516_001207"
     CDS             complement(18177..19121)
                     /locus_tag="VQ516_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2511653.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            19253..22084
                     /gene="uvrA"
                     /locus_tag="VQ516_001208"
     CDS             19253..22084
                     /gene="uvrA"
                     /locus_tag="VQ516_001208"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2511654.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILSPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDNSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPNASKTLHEGALAPWNPISSNYYPNMLEQAMTAFGVAMDKP
                     FEDLSEEDKNLILYGSDGKKFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCHGYRLNDQALSVRVGGEQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            22077..23138
                     /locus_tag="VQ516_001209"
     CDS             22077..23138
                     /locus_tag="VQ516_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2511655.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
BASE COUNT         6997 a         4085 c         4735 g         7372 t
ORIGIN      
        1 actatagtaa ggtgcagcag gataaagaaa aaccgagaag tttctttctc ggatttgctt
       61 attatgaggt tcctattttc tatgctccaa gagttgattg atgtgatcta cttttttagc
      121 ctctctttta tagagaatag cccagatgat gagatagaca atcgcaaact cgataatgag
      181 ctggaggtag aaaatccagt ggaaagggaa ccaaccagca agagttgcta gtgggacaaa
      241 gccagccagc ataaggaaga aatgagtcag agtggcacga agcatgctcc agtcacggct
      301 gaataagcga ttgccaaagt tgaagagcat accaatggct gcccagataa gtgtgcagta
      361 gagcaagacc aaggcaccgt gaacctgatg ctgatccatc acttggccga tgagagagta
      421 gggatttagt ggtgcgtagg tatttggtgc ataaatgagt gaaaagagga tagagaggat
      481 gaggccgata agaacaccgg cagctgcatc gtgaaatact tgttttttca tagttctaat
      541 ttctccttga tggtttttag ataacggcgt gaagagtagg tgaagctttc gtttttcaag
      601 aaaatttcta ccagaccgtt tggcgtgagc ttgaggtgag agatggaatc gatattgatg
      661 atttctgatt gggaaatttg gataaaattg gttggcaaga gtttaagaac ctgatagagt
      721 cgcaaatcaa tgctgtaggt ctgactcgcg gtttctgcta gaaccttccg attctcgata
      781 tagaagcgct gaatcttgcc aatctcaact agatagacct gatcatcgat tttccctttg
      841 attttttctc tttggtccag attttctgcg aactcgatga ctttctgaac tttatctgtc
      901 ggctgaggtg cttggacaat cagcttttcc tcctcgtaag tctcactaat ctgtaattct
      961 actttcataa atttctctcc ttgtttttca tacaaaatta atctcacttc ttgtacacct
     1021 ttattaatac tcttcaaaaa tctcttcaaa ccacgtcagc gtcgtcttgc cgtaggatag
     1081 ttgttgactt tgtcagtttt atctacaacc tcaaaacagt gttttgagct gtctgcgcct
     1141 agctttctag tttgctcttt gatttttatt gagtatacca ctattttact ccctctggca
     1201 aggtactttg tctatgtgga gggattgggc tcctatgtgg tggagctttt ctgttctttc
     1261 tgaaatatgg tataatagca ctaatcaatt tctaggaaaa tagatacaga aaggggctga
     1321 aagaactagg ggaacagttg taattagaag gtgataggta tgaaaagtgg cgttgaagct
     1381 ggataagttt ataaagagtt tgaatgaaga aatgaaaagt aaataagttg atgatgaatt
     1441 gcaaaaatag attttatata caatttgtat ctttcaaatc attgatttga attttagatt
     1501 taatcatgtt acaattaaaa atgaaatcaa atagaagagg aagatgcaaa cgtgggcaca
     1561 ctattagcaa caagattaaa aaatagacga aaagaattaa aaatgtctca gcgagaacta
     1621 gctgaaggta tctgtaaaca ggggcaaatc agtcgattag agagcggaga gtttactcct
     1681 ggagcagatt tcttgcatgc ccttgctaag aagttaaaag tcagtatgga ttattttttt
     1741 gatgagcagg ttgtagagaa agttgaggag ttatcagaat ttaagaaatt agcacagaca
     1801 tttattacaa atcgcaatta tgagtctttg aaatatatat atgaattgga gaatgtaaaa
     1861 gctcaccgcc tatctttagc agataaaatc tacatggaat ggataaaatc tctgatagat
     1921 ttttattttt acggacagaa agaagatgcg attgtacgat tagagggggt attatcacag
     1981 ttaagtgtat ctgatttgaa ctatctccaa gtttcaaaca ccctattcaa tttttattat
     2041 gatattggag atttaggtcg tttcaataaa attcgtgaaa aactagagta tcaagtaaat
     2101 cacctcaatt tgagtacaat cgaagagtta gaactatcta ttaagtttaa ctacaacgtt
     2161 tgtcgttatc tatggttaca gaaaaatatt gaagaagcta ttactaaaat tacagctact
     2221 ataaaacaat gtaaggctta cagaacgaat tatcttttgg ctgatttgta cttattgatg
     2281 ggaaatgtaa gtgaaaattt ttcttctaaa agttcggtga aagaatattt tgagacggca
     2341 tattttctat acaaacttga ggagaacatg tcaatgactc tgaaagttga gcattacatt
     2401 gcagatataa cagaataatg aatacggtgt tcaagtgagc atcgtttttc aaacctagtt
     2461 cagatacaaa aaagtatcaa ataaaaaaaa ataaaaaaac attataaaaa caattgacaa
     2521 gctaattttg aagtgataaa ataaatgcaa gaaaacaaaa ggagaggtat atgagaagtt
     2581 tgtttaagaa aatagttgct gtattagtta ttggcttaat tctgttagga attgcgagaa
     2641 cacctcaagt tcataagatg gctagaggga ttgatcctgg tcctgcgaat tttatttaga
     2701 aatcaatttc tactaattga gtactaattg agtattaatg ggtaaaaaga ttgtatggat
     2761 aagaaaaatt tacttgtatt ggttactggt tcggtaggtg ccagtaatgt agatacatac
     2821 ttatattata taaaaaaatt ctataatgtt aaagtgatac tttctgaaaa ttctaaaaaa
     2881 tttataagta aggaattaat tagttatttt tgtgataaag tttatgatga aatatttgtt
     2941 gaagaggtag ttccgcacgt ttttctgcca tatgaatctg acttacttct tattttacca
     3001 gctacggcaa atgtgattgg caaaattgct aatggtattg ctgatgattt agttacagca
     3061 actgttttaa actttaataa aaaaataatt ttttgtccca atatgaactc tactatgtgg
     3121 gacaatcaca tagttcaaag aaatgtatca attctaaagg agttgggaca tatattttta
     3181 tttgagtcta aaaaaacata tgaggtagga ttgcgtaaag caatagattc aacatgttca
     3241 atgttacaac cacagtcgtt agtaaaagaa cttatcaaat tagaaaatat tgtccttgaa
     3301 gagggacatt aaaaactact gagaatatta aggaggggaa aaaatggaaa attcatcaat
     3361 cgatgtagat atgctgttgg aagaattgac acaagaagca atggtcgttg ttgctgttga
     3421 taaggactgt taatttaaac ttatggcaat atatgaaagg ttactggatg ttttaaatta
     3481 tgcaggcagt agccttttat tatatacaaa tggataaagt aaggataata caatgattaa
     3541 taaaaaaata caacaagttg ttttggaatc attacagaat tttttgaatg ggaacttcat
     3601 ttcgccttgt gtagtctatg attttggctt gctggaaact gtacttgatg aatttaaaaa
     3661 tcaaattcct gtaacattca attaccaact tttttatgcc gttaaagcaa attcaaatga
     3721 gaagatactt gaattcttag tagataaaat tgatggagtt gatgtggcgt cattatctga
     3781 attagatgtg gctaaaaaat ttttcccacc aactcaaatt tctgttaatg gtcccgcatt
     3841 ttcttatgaa actttatata atctgattaa aaaacaatat aaagttgata ttaacttttt
     3901 ggaacatctt caacaatttt ccccaaaaga atctgttgga ataagagtaa cggagccaga
     3961 tgaacttaat aatcgtatga gtcgatttgg aataaatatt tgcagtgata attggactag
     4021 taatttacaa aatcctttaa ttacacgact gcattttcat tttggagaaa aagatgataa
     4081 atttattgtt aagttagata aaatattatt taagttacaa gaaattaata aacttagaga
     4141 ggttagagaa ataaatcttg gaggcggttt tatgaaatta tttatggaaa atcgtttgaa
     4201 agaatttttt ctatcactta tggaaatcta taaaaagtac gatattgata gtactgtgac
     4261 tacaataata gaaccaggta gtgcaattac ttcattttct gcctatatga ttactagccc
     4321 agttaatgtt agtgaggtga atgagcagca ggttatcacg ttagacacat caatatacac
     4381 caatacatta tggtttgttc cgcatattat tacaacgtta aattcaagta gtaaagagcg
     4441 ttatagtact attctctatg gtaatacctg ttatgaacat gacaagtata aaatgaaagt
     4501 ttcgcttcca aggttaactc aaaatagcag tatagtgttt tttcctgtag gagcttatat
     4561 aaaaagcaat cattcaaatt tacatcgtaa tgattttatg cgggaggtat atttgtggac
     4621 aaaaaacttg acatattaga taaagttaag gaatatttag gaaataaaac tactcaaatt
     4681 ctggataatc aatataaaga atttttgaaa cttaatgata taaggcgagc gtttggtatt
     4741 tcagaaaaag tattaaacaa ttcttttaat tttacgagta aagaatttaa tgatttaatt
     4801 aataacgaaa attatttatt cgaatatgca tgtagaatta gagaggaatg gagaaaaaaa
     4861 tgctttaatc attcttatcg ttttctatgc tcacctataa ttacagatga ttttcttaac
     4921 acgaagacat tgagaagtag ccaaattgaa tataaatatg agcgatattt atcgaaaagt
     4981 tcgataggcg atagagcggt tgatggcttt gtttccttca atactttaac agctaatggt
     5041 atgtctgcta ttaaactatg tcttgagata ttaaactcta ttttcttcaa gaagaagatt
     5101 gatttattat attcaaccgg atattatgaa acaagatttt tattaaataa tcttgctaaa
     5161 tcaggtatta gttgctatga ggtaagtaat tgtgaattgg ataaagataa attttataat
     5221 gtattcatga tggaacccaa tcgagccgat ttaacattac aaaaaactga tttcaagata
     5281 gtagaatatt ttgttaagta taaaaataat tcaataaaag tcgttatttt agatatttca
     5341 tatcaaggtt ctaattttaa attagtagaa tttttagaga aatttaaatt tgcgaatgta
     5401 attatttttg tggtacgatc tttgataaaa ttagatcaaa tgggattaga attgacaaat
     5461 gggggaataa tagaagtgtt tattcctaat catttgagaa agttgaaaaa ttttattgaa
     5521 gaggaattca ataaatttag aaattctcac ggagctaatc taagcctcta tgaatactgt
     5581 ttgcttgata attctttaac cttaaaaaat gattggaact attctgattt agttatgaaa
     5641 tttacgagta atttttatgc tgatataaaa gacttgttca tggaaaattc tgatattgaa
     5701 atcatccatg aagagggagt accttttgta tttttagatt taataggtga aggtaaaaaa
     5761 gaatatgaaa tgttttttca atggttaaac ttcttttaca aacagcttgg aatcacattg
     5821 tatgctagaa atagttttgg gtttcggaat ctaacagtag agtattttgg aattattggg
     5881 acagaaagat atatatttaa gatttgtcca ggtgtttata aagggttaag ttattatttg
     5941 atgaaatttt tattaaaatc tttttcaaat gaatatttaa aaactactga tgaggttaat
     6001 agatgaaaaa tttgataaag ttgctaataa ttagattgat tgttaactta gcagacagtg
     6061 tattttatat agtagcattg tggcacgtta gcaataatta ttcttcgagc atgttcttag
     6121 gaatatttat tgcagtaaat tatctaccgg atttgttact aatctttttt ggaccagtta
     6181 ttgacagagt aaatccgcaa aaaattctta taatatcaat tttggttcaa ttagcagtgg
     6241 ctgtaatatt tttattatta ttaaaccaaa tatcattttg ggtgataatg agtctagtgt
     6301 ttatttcagt aatggctagc tccataagtt acgtgataga agatgtgttg attcctcaag
     6361 tggtagaata tgataagatt gtatttgcaa attctctttt tagtatttcg tataaagtat
     6421 tagattctat ttttaattca ttcgcatcat ttttacaggt ggcagtagga tttattttat
     6481 tggttaagat agatataggc atatttttac ttgctctatt tatattgttg ttgttaaaat
     6541 ttagaactag caatgcgaat atagaaaact tctctttcaa atattacaag agagaagtgt
     6601 tgcaaggtac aaagtttatt ttaaataata aattattatt taaaaccagt atttctttaa
     6661 cgcttataaa ctttttttat tcatttcaga cagtagttgt accgattttt tctattcgat
     6721 attttgatgg tccgattttt tatggtattt ttttaactat tgctggtttg ggtggtatat
     6781 tgggaaatat gctagcgcca atcgtaataa aatatttaaa atcgaatcaa attgttggtg
     6841 tatttctttt tttgaacggc tcaagttggt tagtagcaat tgttataaaa gactatactt
     6901 tatcacttat tttatttttc gtttgtttta tgtctaaagg agtcttcaat attattttta
     6961 attcgttgta ccaacaaata cctccacatc aacttcttgg tagggtaaat actaccattg
     7021 attctattat ttcttttgga atgccaattg gtagtttagt tgcaggaacg cttattgatt
     7081 tgaatattga attagtgtta attgctatta gcatacctta ttttttgttt tcttatattt
     7141 tttatacgga taatggattg aaagaattta gtatatatta gaaatgttta tgttcattca
     7201 aaagcataat gactataact gaaaaagaaa agtgatatct ttaaggttgt tcttcttggt
     7261 ggtgagattc gtgagacaac ccaagctttt gtcggaaaga ttaccaatgc tttgatggat
     7321 aggatgtact ttagcaagat gtttttagtg gtaacggtat cgtggatgga cgtgtaataa
     7381 cctcttcttt cgaggagtat tttactaaaa aactagcctt ggagcgttcc ccagaaacgg
     7441 acttactcat tgactcttca aagatttggg gagaagattt tgcttcatct gttccttgaa
     7501 aaaagtcaca gcagtcatca cagacgatag tactgaacaa aactatgaag agttagaaat
     7561 ttatacgcag gtgattgtat aaaggatctg gaaatagata agaagttgat tagtattgac
     7621 ctaggtggta caaatattaa gattactgtt ctttcaaatg acggtgagat tgaaactttg
     7681 tggagtatta caacagatac aagtgagaaa ggttctcaaa ttatatcgga catcatcagt
     7741 tctattaaaa ataaattgac cgaacggaat attcctgata gcgaccttct tggaatcggt
     7801 atgggaagtt gctcatcata ctttccttgt aaatcatagg ggctataaac tctccgtcta
     7861 cttgtcctgc aacaattgaa gtctgctcaa aacgccgtcc gctaatcttt tcatagactt
     7921 tctccctttt aggagcctag ctttctagtt tgttctttga tttttattga gtataccact
     7981 attttactcc ctctggcaag ggactttgtc tatgtggagg gattgggctc ctatgtggtg
     8041 gagcttttct gttctttctg aaatatggta taatagcact aatcaatttc taggaaaata
     8101 gatacggaaa ggggctgaaa gatgtctcat attattgaat tgccagagat gctggcaaac
     8161 caaatcgcag ctggagaggt cattgaacgt cctgccagcg tggtcaaaga attggtagaa
     8221 aatgccattg acgcgggctc tagtcagatt atcattgaga ttgaggaagc tggtctcaag
     8281 aaaatccaaa tcacagataa cggttatgga attgcccacg atgaggtgga gttggccctg
     8341 cgtcgccatg cgaccagtaa gataaaaaat caagcagatc tctttcggat tcggacgctt
     8401 ggttttcgtg gtgaagcctt gccttctatt gcgtctgtta gtgtcttgac tctgttaacg
     8461 gcggtggatg gtgctagtca tggaaccaag ttagtcgcgc gtgggggtga agttgaggaa
     8521 gtcatcccag cgactagtcc tgtgggaacc aaggtttgtg tggaggatct ctttttcaac
     8581 acgcctgccc gtctcaagta tatgaagagc cagcaagcgg agttgtctca tatcattgat
     8641 attgtcaacc gtctgggctt ggcccatcct gagatttctt ttagcttgat tagtgatggc
     8701 aaggaaatga cgcggacagc agggactggt caattgcgcc aagcaatcgc agggatttac
     8761 ggtttggtca gtgccaagaa gatgattgaa attgagaact ctgacctaga tttcgaaatt
     8821 tcaggttttg tgtccttgcc tgagttgact cgggctaacc gcaattatat cagcctcttc
     8881 atcaatggtc gttatatcaa gaacttcctg ctcaatcgtg ctattttgga tggtttcgga
     8941 agcaagctta tggttggacg ttttccactg gctgtcattc acatccatat cgacccttat
     9001 ctagcggatg tcaatgtgca tccaactaag caagaggtgc ggatttccaa ggaaaaagaa
     9061 ctgatgaccc tggtttcaga agctattgca aatagtctca aggaacaaac cttgattcca
     9121 gatgccttgg aaaatcttgc caaatcgact gtgcgcaatc gtgagaaggt ggagcaaact
     9181 attctcccac tcaaagaaaa tacgctctac tatgagaaaa ctgagccgtc aagacctagt
     9241 caaactgaag tagctgatta tcaggtagaa ttgactgatg aagggcagga tttgaccctg
     9301 tttgccaagg aaaccttgga ccgattgacc aagccagcaa aactgcattt tgcagagaga
     9361 aagcctgcta actacgacca gctagaccat ccagagttag atcttgctag catcgataag
     9421 gcttatgaca aactggagcg agaagaagca tccagcttcc cagagttgga gtttttcgga
     9481 caaatgcacg ggacttatct ctttgcccaa gggcgagatg gactttacat catagatcag
     9541 cacgctgctc aggaacgggt caagtacgag gagtaccgtg aaagcattgg caatgttgac
     9601 cagagccagc agcaactcct agtgccctat atctttgaat ttcctgcgga tgatgccctg
     9661 cgtctcaagg aaagaatgcc tctcttagag gaagtgggcg tctttctagc agagtacgga
     9721 gaaaatcaat ttattctacg tgaacatcct atttggatgg cagaagaaga gattgaatca
     9781 ggcatctatg agatgtgcga catgctcctt ttgaccaagg aagtttctat caagaaatac
     9841 cgagcagagc tggctatcat gatgtcttgc aagcgatcta tcaaggccaa tcatcgtatt
     9901 gatgatcatt cagctagaca actcctctat cagctttctc aatgtgacaa tccctataac
     9961 tgtcctcacg gacgtcctgt tttggtgcat tttaccaagt cggatatgga aaagatgttc
    10021 cgacgtattc aggaaaatca caccagtctc cgtgagttgg ggaaatatta aaagtataaa
    10081 aaagtctggg aaaaattttc aaaatcagaa aaacgcataa aatcaggtgt tcaaaaacct
    10141 tgattttatg cgttttatca tggaaatagt tacttcatgt tttcctaatt cttttcgaaa
    10201 ctctttttaa accacgtcag ttttatcagt aatctcaaaa cagtgttttg agctaatttt
    10261 gccagtttta tctgtaacat cgaagttgtg tttgaccact ctgcgactgg tttcctagtt
    10321 tgctctatga ttttcacaga gtattaaatt gcgattttgc caagtttctt tattcgtcta
    10381 aaagtagagt ctgttctatg cgtctaatgt acgaattagg ttgaccattt caatagctcc
    10441 ttgtgcacac tcagaaccct tatttcctgc tttggtacca gctcgttcta tggcttgttc
    10501 aattgtatct gtcgttagca caccaaacat aacaggaatt tcgctattta aactgatttg
    10561 ggcgattccc ttagatacct cgctacatac ataatcataa tgacttgtat tccctctaat
    10621 gacagctccc aagcagataa ttgcatcata ttttttactt tttgccattt ttgatgcaat
    10681 cagtggtatt tcaaaagctc ctggaaccca ggctacctct atatctttct cgtttacatt
    10741 ctctcttttg agattatcta gtgctccaga taataatttt gaagttataa attcattaaa
    10801 tctcgctaca acaataccta ttttaatatt gtttgctact aaattacctt cataagtgtt
    10861 catttatttt tcctccatat ttaaaatgtg acccattcga tttttctttg tttctaaata
    10921 aaaactatcg taaggattgg cttctatttc gattgatatt ctactggaaa tggtaattcc
    10981 atatttttct aactgttcaa ccttgtcagg attatttgtc agtaaatgaa gtgactgaag
    11041 tcccagatct ttaagcattt gtgctccaat atgatattct cttaaatcac cttcaaagcc
    11101 taatgcaaga ttggcatcaa gcgtatccat gccttgatct tgtaaatgat aggcttttaa
    11161 tttattgata agtccaattc ctcgtccctc ctgtcgcaag taaagtaaga cacccgaacc
    11221 attctcaaca atcattttca tagctttatc gaattgctgt ccacaatcgc aacgtaaaga
    11281 gcctaaaaca tctcctgtta aacattcgga gtggacccga cataatacat tggcttcatc
    11341 ctctatattt cccataataa gagcaagatg atgttcccca tttagtttat ctatatagct
    11401 aattgctttg aaattaccgt atctagtagg catattgaca gttgaaactc gttctaccag
    11461 ctgatcatat acttttctat attcttgtaa ttctttgatg gtaattagtg gaatgttgtg
    11521 tttcttcgag aactgaatta aatcatctgt tctcatcatt ttgccatcat gattcattat
    11581 ttcacaacat aggccacact cttttagtcc agctaatttt aataaatcaa cagttgcttc
    11641 tgtgtgtcca tttctttcta gaacaccacc tttttttgca attaaaggaa acatgtgtcc
    11701 tggcctgcga aaatcagagg gtgttatatc ttcagctaca cacatacgtg cggtcagtcc
    11761 tctctcctcg gcagaaatac ctgtggtcgt ttctttataa tcaattgaaa ctgtaaaagc
    11821 agtcttatga ttatctgtat tgttttcaac cataggtgaa agcattaatt gattagctaa
    11881 actttcgctc ataggcatac aaattaatcc tttggcataa gtagccataa aattaacatt
    11941 ttctgttgta gctgcttgtg cagaacaaat taagtctcct tcattttctc tatccttgtc
    12001 gtctataaca agaacaagtc gtcccttctg caatgcttct aatgcttctt gtatttttcg
    12061 atattccatt gactgattat cctttctgct aaaatccatt ttgatataat agttccttag
    12121 atatttctga ttttggagag ttatccatca gtttttgcac atatttacct aagatatcat
    12181 tttcaagatt tactgtactc ccgacttgtt tactcttaag aatggtttgt tccaaggtat
    12241 gagggataac agatactgaa aagtttactt tggagacttt agcgacagtc agactaatgc
    12301 cgtcaattgt aatagatcct ttttcaacta ttaaatctaa aatttctttt tgtgtgttga
    12361 tttgatacca tacagcatta tcatcttttt ttattgacga gatttttcct gtaccatcaa
    12421 tgtgtcctgt aacgacgtga cccccaagtc gaccgttgac agataaggct ctttctagat
    12481 tcacctcact tccatgtttt aatagagtaa gagctgttcg actccatgtt tcattcatta
    12541 catcaactgt aaaggattga tgattgaaat gagtaactgt aagacagata ccatttactg
    12601 ctatactatc gcctaaatgg atatccgtta atatttttga ggctttaatt gatagtttac
    12661 aattacgaga gtctttctgt attctttcaa cttttccgat ttcttcaatt attcctgtga
    12721 acatggataa atcacttcac tttctatgag atagtcattt cctatttgag aaaaagcata
    12781 aggtttcaat ctaatagcgt catttggcaa agaaatgcct tcacctccga caggaaactt
    12841 ggcactgcct ccaaaaattt ttggtgcaat atatattttc agctcatcaa caatttgttg
    12901 ttccaaagca ctccaattca ttagactgcc cccttctaga actaggctat caatctgcat
    12961 gtttcctaga tgttgcatta aactcgataa gtctatatga ttgccttttt tctttatgga
    13021 aagtatttca cagccatgat tttgatatag cttcatttta tttttgtctt cagaggaagt
    13081 ggcaatgtaa gttttaatat catttgctgt ttttacgatt ttagaggtaa gaggagttcg
    13141 taaatgtgta tcgcatatga tacggatagg atttttccct tcctccaatc tacatgtcag
    13201 caaaggatcg tcttgaataa cagtattgac tcccaccata attgcactaa catggtgtcg
    13261 taactgatgc acatgctttc ttgcttcttc ttcagtaatc catttggatt gatttgtttt
    13321 agtggctatt tttccatcca ttgacattgc atatttcata aaaacatagg gtacatgctg
    13381 ggtaatatac tttctaaaac tttttattaa gttaagacac tcattttcta aaattccaac
    13441 agtaacttga agattatttt cctcaagtat ctttactcct tttccagata caataggatt
    13501 acagtctagg cttccaatga ctactcttgt aataccacta tcgattatag catctataca
    13561 gggaggtgtt ttcccgaagt gacaacaggg ttcaagtgtt acataaagcg tcgctccgac
    13621 aggggattct ctacagtttt taagagcatt tctctcagca tgtgggccac caaaaaactc
    13681 atgataacct tgtccgatga tgtgattatc ttttacaata actgcgccga ccataggatt
    13741 gggattgacg taaccagccc ctttttgtgc cagttttatt gctaatttca tatattttga
    13801 atcgctcatc tcactacctc caaaaaaaat ataccttgaa taggggacta ctcaaggcat
    13861 acaaaagaaa acttatgcga ttaacaaaaa tactctgaaa tgacaagtaa tcatttcaga
    13921 gcacgcaaaa agcacaaata tacttttatc ttctttcatc cagactatac tgtcggcttt
    13981 ggaatttcac caaatcatgc ctttcggctc gtgggctata ccaccggtag ggaattacac
    14041 cctgccctga agatagttat tcaattacag atgattatag tacttaattt tgaatatgtc
    14101 aacagataaa taccgattgt ttttgatata ctgtatttgt gataatcgat tctcgctcct
    14161 cggataaaga aaatatgata tactagataa acgaaataag agagaaggaa tactatgtac
    14221 gcatatttaa aaggaatcat taccaaaatt actgccaaat acattgttct tgaaaccaat
    14281 ggtattggtt atatcctgca tgtggccaat ccttatgcct attcaggtca ggttaatcag
    14341 gaggctcaga tttatgtgca tcaggttgtg cgtgaggacg cccatttgct ttatggattt
    14401 cgctcagagg atgagaaaaa gctctttctt agtctgattt cggtctctgg gattggtcct
    14461 gtatcagctc ttgctattat cgctgctgat gacaatgctg gcttggttca agccattgaa
    14521 accaagaaca tcacctactt gaccaagttc cctaaaattg gcaagaaaac agcccagcag
    14581 atggtgctgg acttggaagg caaggtagta gttgcaggag atggccttcc tgccaaggtc
    14641 gcagtgcaag caagtgctga aaaccaagaa ttggaagaag ctatggaagc catgttggct
    14701 ctgggctaca aggcaacaga gctcaagaaa atcaagaaat tctttgaagg aacgacagat
    14761 acagctgaga actatatcaa gtcggccctt aaaatgttgg tcaaatagga gcagagaatg
    14821 acaaaacgtt gttcgtgggt caagatgacc aacccactct acatcgccta tcatgatgag
    14881 gagtggggcc agcccctcca tgatgaccaa gcgttgtttg agttattgtg tatggaaacc
    14941 tatcaggcag gcctgtcttg ggaaacggta ctcaacaaac gccaagcttt ccgagaagcc
    15001 tttcatagct atcaaattca ctcagtcgca gagatgactg acactgaatt ggaagccatg
    15061 ctggagaatc cagctatcat tcgaaataga gccaagattt ttgctacacg cgctaacgcc
    15121 caagcttttc tacagttaca ggcagagtac ggctcttttg atgcctatct ttggtctttt
    15181 gttgagggga aaactgtcgt taacgatgtt cctgattatc gccaagcccc agctaaaaca
    15241 cccttatctg agaaattagc caaagatctc aaaaaacgag acttcaagtt cacaggccca
    15301 gtcgccgtat tgtcttttct acaggctgca gggctagttg atgaccacga gaatgattgt
    15361 gagtggaaag gtcttaaatg atgtctaaca aaaataaggg aattctgatt tttgcgattc
    15421 tctatacagt cctctttgtg tttgatggcg ttaaattgct ggcttcttta atgccatctg
    15481 ccattgcaaa ttatcttgtt tatgtagttt tagctctata tggctccttc ttattcaagg
    15541 atagattgat ccaacaatgg aaggagatta gaaagactaa aagaaaattc ttctttggcg
    15601 tcttaacagg atggctcttt ctcattctga tgactgttgt ctttgaattt gtatcagaga
    15661 tgttgaagca gtttgtgggg ctagtcggac aaggtctaaa tcagtctaat attcaaagca
    15721 cttttcaaga gcaaccacta ctgatagctg tttttgcttg tgtcattgga cctctggtag
    15781 aagaattatt tttccgtcag gtcttattgc attacttgca ggaacggttg ccaggtttac
    15841 taagcattat tctggtagga cttgtttttg ctctgactca tatgcacagt ttggctctat
    15901 cagagtggat tggtgcagtt ggttacttag gtggaggcct tgccttttct attatttatg
    15961 tgaaagaaaa agagaatatc tactatccac tacttgttca catgttaagc aacagcctct
    16021 ccttaatcat tttagctatc agtatagtaa aatgaaataa gaacaggaca gtcaaatcga
    16081 tttctaacaa tgttttagaa gtagatgtgt actattctag tttcaatttg ctgtaatatg
    16141 tgatgaaaat gccagtaatg ataccgagaa aaaagctgag aaacttttcc cagctttatt
    16201 tgttatagtc aaagcgaatg acttgttcct gtgcatctac atgagcatgg accccaaagg
    16261 gtacaattgc tcttggagtt gcgtggccgg cattcagatt atagacaatc gggatattgc
    16321 tgtcaatgat atccaatagt gcctctttat agtcgtcatg gaaagtttca tccataggtt
    16381 ttccgaccaa gagtccattg atgaccgcga atatgccagt gtcctttaaa gttagcaaca
    16441 tctttttgaa gtcttctggc ttaggctttt cttcgcttgt ttcgagcaag aggattttcc
    16501 cttcccagtc tgacaagtca gggaaaagtt tgtatttttg gcagagctcc gtgctatctg
    16561 cgtatcgaga gttgtcaaag atatcgtaga gggattcgag gcaaccaccg aggattttcc
    16621 cctcgaatag tgcccttcct tgcaacaagt caaaacctgt atttgtatga ctgacacgag
    16681 gtgttcccag ggccgtggga ctaaaatcag ttcgttcctc ataccaaacg tcactagggc
    16741 ggatttctga aattcttccc gtctcaatca attctttaaa gtagtgaagg ctataggcta
    16801 gcatttcttt gtctaattca caaatgtctg ctaaaaagga ttgaccataa aaagtcttga
    16861 ttcctaattt atgcaacatg aggtggttca tggttgtatc cgagaagcca agaaaaattt
    16921 tttgcttgat aaccttttgg agttggtcat tttcaaaaag ataaggtagc aagcgatagg
    16981 tatcgtctcc accgatggca cataggatca tgtcgatgct atcatcagaa aaggcatgaa
    17041 tcaaatcctc tgcacgagct tcaggatggt ccttgataaa gtctaatcct tttaacgaat
    17101 ggggcaaaaa gatgggattg agtcccagat ccttgagacg ttggataccc aagtccactt
    17161 cgtgtttgac aaaatcctct ccgataatgc cactagataa actaacaata ccaatagtag
    17221 aaaccatatc tcatcctcct agaaatagat tgagcctatt ttatcacaaa atataagaga
    17281 aagctatgtg ggatttcaga agaatacttt taatactctt cgaaaatctc ttcaaaccac
    17341 gtcagtttta tctgcaacct caaagctgtg ctttgagcaa cctgcagcta gcttcctagt
    17401 ttgctctttg attttcattg agtataactc aaaaaagtaa gtgaaaaagc aaccgcacct
    17461 gcagttgctt ttatcgtttt cttattcttg atagcgtgtt ggtcctgcac ttgcactacg
    17521 gatattcaag cgtttcttga gatagaagcg aagggctagg agggctgctc caataatagc
    17581 tagtggcaat ggatccagta ctgggttaag gctagctggt aggaagcttg ttgcaaagaa
    17641 gacaagcaac caaaggaaca cagaagctag gataactagt acagatttcc agaaaggtgg
    17701 acgttgactg cgatccatat ctggtccata gtattggtaa acaaagtagt acatcaagta
    17761 gaaggcaaat ccaccaacca gtccaactaa tagaagagta atcaatccat agccgaaagc
    17821 ttgatctgcc gcaaagaagg ttgtgagggc gctgacaagg gcaaagaggc tagtgatgaa
    17881 aagagctgag tccataatca tcagttttgg gtcatcattt tcttttggat gctctttttc
    17941 gtactgctct ttgacagtga agctatgagc ccaatgagtt ggtgcgccgt atagggaacg
    18001 ggcagttgta cctttagatt gctcctcaag gatttgggga atgacttcct caaaaatagt
    18061 ctggatttca gcgtctgttt tcccatcttt gatgaattgt tgggtagcaa tgtggacaaa
    18121 ctcttggttt ttcttagtta atttttgtag atcaatttga gacataggaa ctccttttag
    18181 aaccattttt tatggatgag atagagagtg agcgagacac tcatagcaaa ggcgataaag
    18241 acgattaacc agaaggcatt tggctctccg tttaggggaa tttcattatc cttaaagttc
    18301 atcccgtagg cagaaaagac catggttggg atggacatga cgatggtcac aagggccaag
    18361 gttttcatga tgttgttctg gttgttagaa atgatagagg caaaggtctc tgtcatagaa
    18421 tgcaagacgt ttccataaat atctgccatc tcgatggcct gttgggtttc aatcagggtg
    18481 tcttcaagca ggtcttcgtc ctcaaggtat ttcttgatat tgctggttga actggtcaat
    18541 ttcttaatca cgcgctcatt tgttttgagg gaggccttga aatagacgat agttttttcc
    18601 aattccatga gctcaatcaa ttcttcatta cgagttgatt gatgcagttg actttcgatt
    18661 tgttcactct tgcggtcgat tgaacgaagg gctgttaggt aaagctctgc attgcgataa
    18721 agaatttgaa agataaaacg tgaacgcatg aaggtataga aattacgcaa tcgacggttg
    18781 ataaagacat caaggacagg tagtggttcc aaacacgtag tgataatggt ttcctcagtg
    18841 atgataatac caagcgggat ggttacgtag taggtgcggt tatttctttc ctccgtgacc
    18901 ggcacgtcta cgataatcag ggtatactcg tcttcaatgg taatacgaga catttcttcc
    18961 gcatcgagcg gtgctcgaag gtcggcaata tcaatatcga aggtgttggc gatttcgagt
    19021 gattcatttt gagtcggatt gacgagattg atccaagtac ccggttcaag cgtatcgatc
    19081 tctttaaatt cagttgttgt agagagaaaa acttgtttca tatcccttat cctttctcat
    19141 ttttcagatt ttttcacact gtactattat actacaaaat cggccttttg ggtaatagaa
    19201 tctcactttt tttggtataa tggtaagcaa taatggacta gaaagaacaa agatgcaaga
    19261 taaaattgtc attcatgggg cgcgtgccca taatttaaaa aatattgatg tggagattcc
    19321 gcgagacaag ttggttgtcg tgactggctt gtcaggttca gggaagtcca gtttggcttt
    19381 tgataccctc tatgcggagg gacaacgtcg ctatgtagag agtttgtccg cctacgctcg
    19441 tcagttcttg ggaaatatgg aaaaaccaga tgtagatgcc attgatggtc tcagcccagc
    19501 tatttctatc gaccagaaaa cgactagcaa aaaccctcgc tcgacggtgg gaacaacgac
    19561 tgaaatcaat gactatctgc gtctcctcta cgcgcgtgtg gggacgcctt actgtatcaa
    19621 cgggcatggg gctatcaagg cttcttctgt ggagcaaatc gttgataagg ttttggagtt
    19681 acctgaacgc cagcgcttgc agatcttgtc tcctgtcatc cgcaagaaaa aaggccaaca
    19741 taagagtgtt atcgagaagg ttcagaaaga cgggtatgtt cgtgtccgtg tggatgggga
    19801 agtctatgat gtgaccgaag tgccagagtt gtctaagagc aagcaacaca atatcgatgt
    19861 cgtggttgac cgtattgtta tcaaggaggg cattcgtagt cgtctctttg attccattga
    19921 ggctgccctt cgtattgcag aaggttatgt cattatcgac accatggaca actctgagtt
    19981 gttgttctct gagcattatg cctgtccagt ttgtggtttt actgtcccag agttagagcc
    20041 tcgtctcttt tctttcaatg ctccttttgg ctcttgtagt gagtgtgacg gcttgggcat
    20101 caagctggag gtggatactg atttggtagt gccaaatgcc agcaaaacct tacatgaggg
    20161 agcgctggca ccttggaatc ctatctcatc caactactat ccaaacatgc tagagcaggc
    20221 catgacggct tttggagtgg ctatggataa gccttttgag gacttgtcag aagaagacaa
    20281 gaacttgatt ctctacggct cagatggtaa gaaattccat ttccactatg agaatgaatt
    20341 tggtggcgtg cgcgatatcg acattccgtt tgagggagtt atcaataata tcaaacgtcg
    20401 ttaccatgaa accaatagcg attacactcg cactcagatg cgcctctaca tgaatgagct
    20461 gacttgcgga acctgtcacg gttatcgtct caatgaccag gccttgtctg tccgtgtggg
    20521 tggtgagcaa gggccacata tcggagaaat ttcagacctg tctatcgcag atcacttgga
    20581 cttggtgagc cagttaactt tgtctgaaaa tgaagccatc attgctcgtc ccattctcaa
    20641 ggaaatcaag gatcgtttga ccttccttaa taatgtgggt cttaactatc taaccctgtc
    20701 acgttcagca ggaacccttt caggtgggga aagtcagcgc attcgtttgg caacccagat
    20761 tggttccaac ctatcaggcg tcctctatat cctagacgag ccgtcaatcg gtcttcacca
    20821 gagagacaat gaccgcctga ttgccagtct gaaaaagatg cgtgacttgg gcaatactct
    20881 tatcgtggtg gaacacgacg aagataccat gcgtgaagct gattatctga ttgacgttgg
    20941 tcctggtgcc ggtgtttttg gtggtgagat tgttgcagca ggtacaccta agcaagtggc
    21001 tcgcaacagc aagtctatca caggtcagta cttgtcaggc aaacgtgtca tcccagtacc
    21061 agaagagcgc cgtgtcggaa atggtcgttt tattgaagtg ataggagcgc gtgagaacaa
    21121 cttgcaaaat gtcacggctc gctttccact aggaaaattt atcgcagtga caggtgtgtc
    21181 gggttcaggg aaatcgactc taatcaacag cattctcaaa aaagccattg cccagaagct
    21241 caaccgtaat tcagacaaac ctggtaagtt taagactatt acagggattg agcatgtcga
    21301 ccgtttgatt gatattgacc agagccctat cggacggacg ccgaggtcta acccagctac
    21361 ctatacagga gtttttgacg atatacgtga cctttttgct cagacaaatg aagccaagat
    21421 tcgaggctac aagaagggac gcttcagttt caacgtcaag ggaggtcgct gtgaagcttg
    21481 ctcaggtgac gggattatca agattgagat gcacttcttg ccagatgttt atgtggcttg
    21541 tgaagtttgc cacggaaccc gctataacag tgaaacccta gaagttcact acaaggaaaa
    21601 gaatatttcg caggtcttgg atatgacggt caacgatgcg gtagaatttt tccaacacat
    21661 tccgaaaatt caacgcaaac tccagaccat caaggatgta gggctaggct atgtgacctt
    21721 gggacagcca gctaccaccc tttctggggg agaagcccaa cgtatgaagt tagctagcga
    21781 actccacaaa cgctcgacag gaaaatcttt ctacattctg gatgaaccga cgacagggct
    21841 tcatacagag gacattgctc gcctgctcaa ggttttagct cgctttgtag acgatggcaa
    21901 tacagtcctc gtcatcgagc acaatctgga tgttatcaag acggcagacc atatcattga
    21961 cttgggacct gagggcggtg tcggtggtgg aaccatcatc gtaacaggaa ctccagaaga
    22021 agtagctgcc aacgaagcca gctatacagg acactatttg aaaggaaagt tacatcatga
    22081 ataaacgcgt acaagcattt ctagctaaaa tgcaagaaaa agaactagat ggtgtcatca
    22141 tcaacaatct taaaaacgtc tattatttga ctggtttttg gggctcaaac ggaacagtct
    22201 ttatcagccg tgaccgtcag gtattagtga cagactctcg ctatatcatc gcagctaagc
    22261 aagaaaccag tggttttgag attgtggctg atcgtgatga attggctgtc attgcaggaa
    22321 ttgttaagga catgggcttg actcgtatcg gttttgaaga tgagatttca gtgtcttatt
    22381 accaccgtat gcaggcagct tttgcaggtt tgaacttgct tccacaaact cagtttgtgg
    22441 aaggtcttcg tatgattaag gatgaggcag agattgcagc gattcgcaag gcttgttcta
    22501 tctcagacca agctttccgc gatgcgcttg actttatcaa accaggaaaa actgaaattg
    22561 agattgccaa cttccttgat ttccgcatgc gtgagttggg agcatctggc ttatcttttg
    22621 atacgatcct agctagcggt atcaattctt ctaaacccca tgcccatcca atgcacaaac
    22681 cagtggagtt gggagaagcc attaccatgg acttcggctg tctctatgac cactatgtca
    22741 gtgatatgac ccggactatc tatctagggc atgttagcga tgagcaggca gagatttaca
    22801 atacggttct aaaagctaac caagccttga ttgaccaagc taaggcaggc ttaggtttcc
    22861 gtgactttga caaaatccct cgtgatatta tcattgaggc aggttatggt gactacttta
    22921 ctcacggcat tggccacggt attggtctgg atatccatga ggaaccctac tttagtcaga
    22981 cttctacaga aactattaag acaggtatgg ccttgaccga tgaaccaggt atctatatcg
    23041 aaggcaaata tggcgttcgt atcgaggatg atatcctgat tacagagaca ggttgtgaat
    23101 tattgaccct agctccaaaa gagttgatag tcatttagtt gtttgtcaag aaaaaagtac
    23161 gaaaatgacg aaaaaagtca aaaaaaatt
//
LOCUS       DAVBCV010000031        22356 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBCV010000031 DAVBCV010000000
VERSION     DAVBCV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22356
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.031"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(160..2118)
                     /gene="ftsH"
                     /locus_tag="VQ516_001210"
     CDS             complement(160..2118)
                     /gene="ftsH"
                     /locus_tag="VQ516_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2511656.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNREIHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2134..2676)
                     /gene="hpt"
                     /locus_tag="VQ516_001211"
     CDS             complement(2134..2676)
                     /gene="hpt"
                     /locus_tag="VQ516_001211"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2511657.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2680..3957)
                     /gene="tilS"
                     /locus_tag="VQ516_001212"
     CDS             complement(2680..3957)
                     /gene="tilS"
                     /locus_tag="VQ516_001212"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2511658.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3954..5222)
                     /locus_tag="VQ516_001213"
     CDS             complement(3954..5222)
                     /locus_tag="VQ516_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2511659.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5342..5710)
                     /locus_tag="VQ516_001214"
     CDS             complement(5342..5710)
                     /locus_tag="VQ516_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2511660.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5703..5969)
                     /locus_tag="VQ516_001215"
     CDS             complement(5703..5969)
                     /locus_tag="VQ516_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2511661.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(6027..9536)
                     /gene="mfd"
                     /locus_tag="VQ516_001216"
     CDS             complement(6027..9536)
                     /gene="mfd"
                     /locus_tag="VQ516_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2511662.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YQKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPIQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(9537..10106)
                     /locus_tag="VQ516_001217"
     CDS             complement(9537..10106)
                     /locus_tag="VQ516_001217"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2511663.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10177..11292)
                     /gene="ychF"
                     /locus_tag="VQ516_001218"
     CDS             complement(10177..11292)
                     /gene="ychF"
                     /locus_tag="VQ516_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2511664.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIIEFRFNV"
     gene            complement(11376..11570)
                     /locus_tag="VQ516_001219"
     CDS             complement(11376..11570)
                     /locus_tag="VQ516_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2511665.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11635..12771)
                     /gene="dnaN"
                     /locus_tag="VQ516_001220"
     CDS             complement(11635..12771)
                     /gene="dnaN"
                     /locus_tag="VQ516_001220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2511666.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12930..14291)
                     /gene="dnaA"
                     /locus_tag="VQ516_001221"
     CDS             complement(12930..14291)
                     /gene="dnaA"
                     /locus_tag="VQ516_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2511667.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14504..15262)
                     /locus_tag="VQ516_001222"
     CDS             complement(14504..15262)
                     /locus_tag="VQ516_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2511668.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15320..16501)
                     /locus_tag="VQ516_001223"
     CDS             complement(15320..16501)
                     /locus_tag="VQ516_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2511669.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16684..17163
                     /gene="rlmH"
                     /locus_tag="VQ516_001224"
     CDS             16684..17163
                     /gene="rlmH"
                     /locus_tag="VQ516_001224"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2511670.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17207..17280
                     /locus_tag="VQ516_001225"
     tRNA            17207..17280
                     /locus_tag="VQ516_001225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17241..17243,aa:Arg,seq:tct)
     gene            17445..17570
                     /gene="comC"
                     /locus_tag="VQ516_001226"
     CDS             17445..17570
                     /gene="comC"
                     /locus_tag="VQ516_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2511671.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            17591..18916
                     /locus_tag="VQ516_001227"
     CDS             17591..18916
                     /locus_tag="VQ516_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2511672.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18913..19665
                     /locus_tag="VQ516_001228"
     CDS             18913..19665
                     /locus_tag="VQ516_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2511673.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19717..19788
                     /locus_tag="VQ516_001229"
     tRNA            19717..19788
                     /locus_tag="VQ516_001229"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19750..19752,aa:Glu,seq:ttc)
     gene            19794..19867
                     /locus_tag="VQ516_001230"
     tRNA            19794..19867
                     /locus_tag="VQ516_001230"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19828..19830,aa:Asn,seq:gtt)
     gene            complement(19908..20450)
                     /locus_tag="VQ516_001231"
     CDS             complement(19908..20450)
                     /locus_tag="VQ516_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2511674.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20629..21396
                     /locus_tag="VQ516_001232"
     CDS             20629..21396
                     /locus_tag="VQ516_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2511675.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21481..22161
                     /locus_tag="VQ516_001233"
     CDS             21481..22161
                     /locus_tag="VQ516_001233"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2511676.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFLLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
BASE COUNT         6919 a         4115 c         3650 g         7672 t
ORIGIN      
        1 acagtccctt ggacttcttc atacaattct ttaatcataa ttttcccctt tctagataca
       61 gtctaacaga ttagaaaaca catttgttcc attttctcca ttctgttccc tttagctcct
      121 aaactgcaca aaaaagagag gctccagcct ctctcagggt tatttttcgt cattcatttt
      181 tgactttact tcatcatagg atagtgcatg agattcctct tctactgctt caggcatctt
      241 tcctgtttcg taaagagctt taatttgtgt actatccaat gtttcgtatt tcaataatgc
      301 ttctgcaatt aacttgtgaa tttcacgatt tgactgaata atttcagcag ctttatttcg
      361 tgcctcattt aataatgaac gaacctcttc atcaatttca taagctgttt gttctgaaat
      421 tgatttttga ggactctgtg caccaaacat agcatggttt ccttcatatt gtactgggcc
      481 aagtttttca ctcataccgt actctgtaac cattgcacgc gccatttgtg tcgcttgttc
      541 aaagtcgttt gaagctcctg tagtttggac attaaagata atttcttcag ctacacgtcc
      601 acccattaag ccagccaatt gctctttcat atcttcttta gatagaagca tttgatcctc
      661 tttaggaagt gcaatcatgt atccgcctgc acggccgcgt ggtacaattg taaccttatg
      721 gacaacgcga gcattcgaca agactagacc aacaatggta tgtcctgcct catggtaagc
      781 aaccaattct cgttcttttt gtgaaactgt cttatctttc ttagaaggtc cagcaataac
      841 tctatcttct gcttcatcaa tatctgaagc atcaattatc gatttattgc gacgagcagc
      901 aactaaagct gcttcattca agacattctc taaatcagca ccaacaaagc ctggagtttg
      961 ttgagccact aatttcaaat caacatcttc tgctaaaggc ttgttcttag cgtgaacttt
     1021 caagattgct tcacgacctt taacatcagg acgaccaacc aatacttttc tatcaaaacg
     1081 tcctggacgc aaaagggcag gatcaagtac atctgaacgg tttgtcgcag cgatgacgat
     1141 aatcccttca tttccctcaa aaccatccat ctcaatcaaa agttggttca aggtttgttc
     1201 acgttcgtca ttacctccgc cgagaccgac tccacgttga cgtccaacag catcaatttc
     1261 atcgataaag atgatagctg gtgctgcttt tttggcatcc tcaaaaagag agcgaacacg
     1321 actagctcca actccgacaa acatttctac aaagtcagaa cctgagatac taaagaatgg
     1381 aacacctgct tctccagcga ctgccttagc aagcaaagtt ttacctgtcc ccggaggtcc
     1441 ctccaaaaga acacctgctg gaatacgggc tccaagtttt gtgaatcgtt ttggatcttt
     1501 taagaactca acaacttcaa ctagttcttg tttttcttcc tcagctccag caacatctga
     1561 aaatcttact ttaatatctt ctttatttgc tgctttagcc ttactacgtc caaaactcat
     1621 tggattacgg ccattgcctc ctcccatatt tcccatcata gagaatagga agaagaatag
     1681 aattccaaat ggcacaatgg atacgagtag attaatccat ataccacttg aactttcatg
     1741 cttaacagtt acttctgctt tatggtcagt agcaagtttt tgcaattctg atacggtagt
     1801 atctgcagga agaatagtgc tggtaaattt ctctacctta gtaacagatg gcgtgaaaaa
     1861 ctgaatacct gtttcttctt tacttgtttt aggattttta tagacaccag aaacttcgat
     1921 aacactacca tttggttggt aagttaattc ttttacatta ccatcggtaa tttcttgtac
     1981 caactcagta tagttgattt gctgacttcc tcctgagtta ttcccagaat agaaatactg
     2041 gaatcctgtc acaaggaaaa agataaataa taaccataga aaaggatttt taattaaacc
     2101 attattttgt tttttcatta aagattattc tttctaattt gaatacactt cctctttcaa
     2161 tactccaata taaggaagat tacgataatt ttctttgtag tctaaaccat aacctactac
     2221 aaactcattt gggatagtaa agcaggtata gtctgcctca atttctacaa cacgtccttc
     2281 tggtttatcc aacaaggttg caattttaac agaagctgct tctcttgctt taaacatatc
     2341 tcgcaaattc ttcaaagttt gacctgtatc aatgatatct tctacaaata gaacatgtct
     2401 tcctttgata tcttgagtca catcttgttt aatattgata acaccactac ttgctgttcc
     2461 accatggtag ctagaaacca tcatgaagtc catttcaata tgtgtatcaa tatgtttgac
     2521 caattcagcc ataaaaggaa tagatccttt taaaatccca actaagattg gattttttcc
     2581 tgcatagtct ttagttaatt gagcacctag ttttttagct gcttctgtaa tttcatcgtg
     2641 tgaaacgagg acttttttaa tatcgttttc taacattttt tacctatcta ttttttctat
     2701 ataaagtaca gtgttcatta tatcattttt cgttttttta ctcaaattat tggtcgcaat
     2761 tcccaaaatt gagacaattt caccaaattg ctcaataata agagcagagt ttctcttttc
     2821 catagggatt ttcaaatcaa taaataaacg tctgagtttt tttctatgcc cattttgaat
     2881 caaaacatct cctgtttttc gatgacgaat gtgtatggat gtttcacgtg aaacaggtat
     2941 ttgttgaatt gattcacctt ctaatggaag tccaaaggaa aataaatatc cttgataagc
     3001 tacctgattt tgatagtgta acacaagttc atcttcctct tcatcagcct gcggactgat
     3061 tttacaaatc tgaaactgtt ggtactcttt tatcaattca tagccatttt taatcggatg
     3121 acgatactgg cttttagatt ttaaaatctg ctgaacttca gcaaactgag cttttgtaag
     3181 attcaaatct ggaaaacgat tcagataagt ttgaagtaaa actctttgtg tagactcaga
     3241 gtaagaaaat aactgctgta aatcttccac attaatattg ttagataatt cagctattgc
     3301 caaatcataa tctaaaattt cattgccaat gcctaagatt gcatccctaa atcgaggatt
     3361 ttctttttcc aattctggta agtaagagtt tcgaatacga tttcgaaaat aatgattctc
     3421 ctgatttgat gtatcttcaa agtgaaaaat tgatggaaag tcttttttct gaaaatgcaa
     3481 gaagggacga atgatttcta tctctccgac tacttgcttc tccttaattc ctgatagata
     3541 gcgcaagcga gttcctcgaa tcaagcgcat caaaatcgtt tccacctgat catcagcatg
     3601 gtgggcagtg actaaagctg tcgcacctgt ctttttcatg acctcttgaa aaaaatcata
     3661 acgaaaattt cgtgcacgcg cttctgaaaa ttctcctgaa aaattgctga tataaatagg
     3721 aagctctgct tcagcagcca acttccttaa ttccttttct tcccaatctg attcaattct
     3781 ctgcttatga ttcacatgag ctagaatcaa ttcaatctct aactcttttt gataagtaga
     3841 caatacctta aatagaaaca tggaatctaa tccaccagaa agagctagaa ccgccttagc
     3901 atgctttttg aaatatccct tcttgagaaa atgatttaaa aaatctggtt ccctcatttt
     3961 agaacctcat aaacatcctt ggctatctta gaaatcgtat cataatcaga attcttagtg
     4021 aaaatagaaa gaataaatgg agaatctgca tagacaacac ccgtatcatg cttaaattca
     4081 tccgcatctc caattttatg agctacttta acagaaacac ctttggcaat tcgctgacta
     4141 tcaaaatctg ttttagtcaa agactctaac acaaatccat tttgattata aatagcttcc
     4201 ataaacttcc cggccatctt agaagaaatc aatttttctt ttggatccca atcatctccc
     4261 ataatggcag acatcttgga tttgaatgtg gcatcagatt ggtttgaaat gtaatatccc
     4321 aatagattat gagctacatt atcagattct tttgatactt tcgtaattaa atcctttaaa
     4381 gaatattctt tattatcttc ttttttagga agactaccac ttccctctgg tttataagaa
     4441 cctggaaaat cattgactgc agatacgtat tttacagtcg tatctaactg ataaagaccc
     4501 tcatttattt tttcttgcgt ataatagaga taagagagtt tcaaaacgct ggctgcatac
     4561 atcttttcat cttgattgat accagcttct tttccagtag tcagttgctt aacataaata
     4621 gagaaagaat ctttctgata tttttcagat aacatttctt gaactttact catccgatta
     4681 tcttcttcag aagttgattc tttagctacc catccagcct gatcaatatg tagaaattct
     4741 cttccttcta caaacatggt cttgtcgatt gatacttgcg aataagctga taaggatgat
     4801 ttcacttctt ttaaatcata aggactattg tacagtttaa agtcagattc taaccatgct
     4861 ttttttattg ttggagttac ctctgattga tcatataaaa atcgtttgtc cgcagctata
     4921 aattgatgat ttgatagctt aaatactgga attccttgtt tatttaagcg ccactcggtt
     4981 atttgaaaac ttgttttggg agtcaatttc ccagattcca ctactaaatc ttcattcgca
     5041 taaacaggaa cctctccata aaccatggga gaacttaatt tttctctaaa ataaatacca
     5101 aagtcagatt gtgaaaggta ataaatttct ttcgaagtat agacgacttc tttttctgtg
     5161 ctaacgactt ttgaaatggt caaaaaactt ggtagcaaca aaataattaa gaatttacgc
     5221 attcttcctt tccttttctt cttgtaaacg caataattga tttttatcag ccaattcttc
     5281 cagcttttca tctgacaaac tcaaaaattg ctctattacg tctaataaat tttccatttt
     5341 atcacctttg aagcaagtca ggaatcgtat aaactttttc cctcgactta gaataatagt
     5401 acttcgctcg tgtatatttg gcagcataat cttcatcttt caacttggta gcaaatgctg
     5461 tctccttatc cttttcatca ctcaaagttt gatactgagt ttgcaagtct gctaattgct
     5521 gacgtctttg gagtaattgc tgataactct gcgctaaatt aaaagttggc aagataaata
     5581 atagcataat caaaatcaat acccctccca taaaacgatt ccgtttttgt cgttctttca
     5641 tcaggtagcg acgacgttgg tattcatttt gaataaaaga attattcaat tgtacaatat
     5701 ttttagacat tttcttctac ccgtgtttca ctgataattt catacattcc tgctgcatct
     5761 tctttttttg tactatcttt catctctagt acttttacaa gcagcaactt attgccaaag
     5821 cgaatttcaa cttggtcatt aactttcaag tccgttgaac ttttggccaa gattccatta
     5881 accttgattc tacctttatc tgctacttcc tttgcgactg tacgacgctt gataattcgc
     5941 gatactttta aatatttatc taatctcatc cttattacct caatttatta ttgtaccatt
     6001 tttatctatt ttatagaaga aaaatatcaa atggaatttt cttccttaaa ctcttttatc
     6061 tctaataaac tttctccaaa aatcagcaga ccttctaaaa tttcataatc tttcttattt
     6121 tggacatcaa atacaagctc cattaatccc ttattctcag cgatgcctgc ttttaagttc
     6181 gttacggata aagctttaaa ataatcttga gctaaaaaca atcgttgagt gactttttca
     6241 aattgaattg taattttatt atcttttctt tccacacgtt gaacaaagac cttgtccaag
     6301 tatgatttaa ccaaaccaat ctctaaaaga taggctacta catctgggta ttctccaaaa
     6361 cggtctatca actcctcttg taactcttca taattgacac ggttgtcaat ttgacgaatt
     6421 ttcttgtaaa tttcaatctt atgtcgttga tcagaaatat aagtatcagg aagataggca
     6481 tcaatttgca aaatcaactc agcattccct ttggttcttg tgttagcgtt agcattaccg
     6541 tttcgtttag caatagcttc ctctaataac tgcgaataca attcaaaacc aacagaatca
     6601 atgaaaccag actgggattt tcctaaaaga tttcctgctc cacgaatcga aagatctcgc
     6661 attgcaatct taaagccaga gcccaattct gtaaatcctt taatcgcttc taatctcttt
     6721 tcagagactt cactgattga tttttctgga cgatacatga gataagcata agcaatacga
     6781 ttactacgac cgactcttcc tcttaactga tataaggttg acaagcccat atggtccgca
     6841 ttttcaataa ataaagtatt agcatttgga atgtccaccc ctgtctcaat aatagtagtc
     6901 gtcaccaaga tatcgtattg tccctcaata aagtctaata gagtattttc caactggact
     6961 tcactcattc gaccatgaac atatccaatc gaagcctccg gaatcaactc ctgtaattct
     7021 gaaaccttct gaacaattgt gtcaactttg ttgtaaagat aataaacttg acctccacgc
     7081 tccatttcac gcaagacagc atcacgaatg acactatcat tcttttccaa aacataggtc
     7141 tgaacaggat agcgattagt cggcggagtt tcaataacag ataaatctct gattcccagc
     7201 atagacatat ggagggtacg agggattggc gtagcggtca aggttaggac atccacttgt
     7261 ttcttcagtt ctttcaaagt ttccttatgc ttgacaccaa atcgctgttc ctcatcaata
     7321 atcatcaagc ccaaatcagc aaacacaaca tcttttgaca aaacacgatg tgttccaatc
     7381 aaaatatcga cttgaccgtt tttcaatttt tcaagtgttg cagtctgctc ttttttactt
     7441 ctaaagcgac tcaacacatc aatattaact gcaaaatttt ggaatcgttc cttaaaattc
     7501 gtatagtgct gttgcgctaa aaccgtcgtc ggaactagaa tgacaacctg tttgtgatca
     7561 ttgactgctt taaaggctgc acgcatagca acttcagtct ttccaaaacc aacatcccca
     7621 actaaaagtc gatccattgg ctgagaagcc tgcatatccc tcttgatttc ctcaatacta
     7681 cgaagttgat catccgtttc aacataaggg aaagcatcat caaaggcatc ttgatcatca
     7741 tcatcagctg agaaagcaaa acccttcaac tgactacgtt cagagtagag tttgattaaa
     7801 tcatcagcta tatcctctac ctggttctta accttttgct tggccttttt aaaatgaccg
     7861 tcatttaatt tattgagttt tggagcttta ccatcacttg aaatatattt ggacagtaga
     7921 tgaatctgtt ccacggggat agaaatttga tcaccatttt ggtattggac actgacataa
     7981 tcgcgatgaa ttcccttgat ttcaatggtt tcaattccta gatattgacc aatcccatgg
     8041 atatgatgga caacatagtc ccctttttca agttcattgt aatcttttaa tctctctgca
     8101 tttgaaacat gttgtcttcg aaaacgacgc tttaatttct tttgaaaaat ctcatgttca
     8161 gttatcaata aaatcttttc atctacaaaa tgaaaaccat gtctgagatt accctctatt
     8221 aaatttacag attctttaca gatacttgtc ttatctctag aatccaattt aatctgatat
     8281 tcctctaaca tatcctccaa tgttttactt cccattgaat tgctagactg cagaataatg
     8341 gtgtaatcca tttttttata tcgttcaatt tcttctttta gaaaagaaaa ctgattgaaa
     8401 aattcctgta taggatattg attgaattga taaattttgt caaatttgag atttcctaaa
     8461 cccttttgaa gattagagaa aaaggtcact ggactttgtt ttttatagat ttgttcaata
     8521 tcagaaaaat actgcatatc agaaaatgct ttactattct gtaattcttc tgtaaagtac
     8581 tgcgctaatt ctctttcaaa gacttcatac tgattcatca atttttgata atcatcaaag
     8641 aatattggag tatctttttc aatataatca aagacagtcc atgtcttatc atagcacaaa
     8701 gataaaaact tccgagaatc tgcatgactt tgtttttggt gaaaacttga aagaatttct
     8761 tctaggtatg atttcaaaat aggtgataaa gtttttgaaa tttgtttttc taaagctgac
     8821 tgtcctcgtt gataatcctt ttctctcaaa agcatatcac tagctggaaa gatagtgagt
     8881 tctgtcttat tttctttcga taattgtgtt tctacttcaa atgacctgat accatcaatt
     8941 tcatcaccaa aaaactcaat tcgacaaggt tctaactggg atatttcaaa aatatctaaa
     9001 atatctcctc gaagactaaa ttcgccctga gtttgtactt gagtaacttt ttgatagcca
     9061 ttttccttta actgatggat aaacgcgtgt tgagcatatt cttcaccaac tgagattttt
     9121 acaatactat ctttgaatgc attgggagac ggtaaaatca atcgacttgc tgcgatatta
     9181 caaactaaaa tccctttctt agatgaatca gtcaaaaaac gcaaggcttc aacccgtgaa
     9241 ataatttttt cctgtgaaga catcaaaaac tccaccatag gagcatcatc taccaaaaat
     9301 ggatagacga gttcctcacc caagatagaa ataagatcac taacaagtcc ttctgcttct
     9361 ccataagttg acgtcaataa cacaatccta tcttcttttt ctaaactgct tgcaattgca
     9421 agagccttag tagatgttga taaaccaagt attagttgtc ttttcttatc tgttaaattt
     9481 tgatgccatt ttttaatctg atcattttct gagaataaat ctaataaggt caccatttat
     9541 ccgttatacc tctgcattgt tttctcaaaa tttttctctt gtaaatagta ttttacagaa
     9601 tcgtcaactt tgtcaacaga ctgtaaaata ccgatataat catccctgtc aaacttactc
     9661 aaaacatgat gaacaactga cataccattt ttaggtcttc caattccaat cttaacacgg
     9721 ttaaagacct gagttcctat atgttgaata atagacttga taccattatg accacctgct
     9781 gagccttttg ctcttaaacg aattttccca acttccatgt caagatcatc gtaaatgata
     9841 agtaaatcgt caatatccaa accatagtaa gttaataaag catgaactgc ttttccactt
     9901 tcattcataa aggtcgttgg tttaaccaga taaatttttt ctccatttag gaaaaaggat
     9961 gctaggtcag cttgaaatat cttatcgtgt gtaaaagtga cattctgttt cttcgctagt
    10021 tgatcaatca acataaaacc aacattgtgt tttgtttcaa aatatttatc ccctggattt
    10081 cccaaaccta caagtaattt ggtcatttat ttttcctttc aaaagccaaa agggttggaa
    10141 tttttttcca acctaattga caccatttat taatttttag acattaaagc ggaattctat
    10201 gatgtcgcca tcttgaacga tatattcttt tccttcttca cgcaagcgtc cagcttcttt
    10261 tacggccttt tcagatccgt atttcactag atcttcatat gacatggtta ctgcacgaat
    10321 aaagcctttt tcaaagtctg agtggataat accagctgct tgaggagcct tcataccacg
    10381 tttgaaagtc caagcgcgaa cttctttttc accagctgtg aagtaagttc ccaatccaag
    10441 caagtggtaa gctgcacgcg tcaacttatc tacacctgat tctgtcaaac caatggcttc
    10501 aagaaactct tttttatctt catcatccaa ttcagaaatt tcttcctcag cacgcgcaga
    10561 aataacgact acttcagcat tttctgtcgc tgcaaattca cgaatttgtt tgacatagtc
    10621 gatagagtca ggttctgaaa ccacatcctc gtccacatta gctacataaa gaactggttt
    10681 agtcgtcaaa aggaaaagac ctttgacaac cttttgttcc tcatctgtaa attcaatggt
    10741 acgagctgat ttcccgtctt ctaggactgg tttaatcttt tgaagaacat tgaattctgc
    10801 tactgattct ttatcttttt gcgtacgtgc catcttttct acacgcgcat atcgtttgtt
    10861 cactgattct aagtcagcaa gaattaattc cagattaatt gtatcaatat ctgcaagtgg
    10921 atctacaaag gcgtcttcac gtccttgctc gcgcatcaca ttttcatcat caaaagcacg
    10981 aactacgtga acaatcgcat ctacttcacg aatattggcc aagaatttat tccctagccc
    11041 ttctcctttt gaagctcctt ttacaatccc tgcaatatcc gtaaattcaa atgttgtggg
    11101 aactgtcttt ttaggagtta tcatttcagt tagtttttgt aggcgttcat ctggaacttc
    11161 caccattcca acatttggat caatcgtcgc aaatgggtag tttgctgcct ctgctcctgc
    11221 ttttgtaatt gcattaaata gtgttgattt accaacgttt ggcaaaccaa cgatacctgc
    11281 tgtcaaagcc atatttctca ttctccgttt tcatttcaat ccctaatatt ataacacaaa
    11341 aagggataaa gtgctaaccc tctaactaag ggttctcagt caataatttt attcattttt
    11401 cgctcaaaat catatcgccc catcatgaca acatgctcac aattactgca ttttattttg
    11461 atatctgctc ctacacgtgt aatttcccaa cgattagcct ttttaccagt cgacttgatt
    11521 gtacaagcgt gtgatttttt catctcaaca aaatttccaa cttgatacat actactctcc
    11581 ttttcttttt cgattatatc ataaaagagg cgagccaggc tcaacctctt tcacttaatt
    11641 tgtacgaact ggtgtaatga gctgcatgaa gtcttcgtca gtatctgctg gcacaagagt
    11701 aaatggacga acagctgaga taaagctaat ggtcaccttt tcgctattta aagctttaag
    11761 agaatcaatc aagtaagttg ggttgaaact aatggtcaaa tcttcaccag taacctgatc
    11821 agtatcgatt tcttcgttta ctttaccaac ttctggagag tgaacatggg cgctaacaac
    11881 cccatcctta atttcaagtt tcacagtacc attttgagtc gcacttgata aaagacgggc
    11941 acgctccatt gactggcgta agtttaccac attaaaagta atagtagtgt taaagtctgt
    12001 tggaatcaag cgatctgtat caggatagtt tccttctagg agacgagtat agaagctaat
    12061 attttcgctt ctaaagagga tttggttatt ggcaaagaaa atctctacag tttcgatatc
    12121 atctgtaaat accgctgaaa attcgcgtag agaacggcta ggaattacga catcaaaatc
    12181 atcactattt ttttcaagag tcaatttttt ctggcttagg cgatgagagt ctgttgcaac
    12241 tgtttttaac tctttgtgtt gactcaatac gaagtggaca cctgttaaaa tcggacgact
    12301 ctcttgtgta cttgcagcaa aggctgtttc attaataatt ttcttgagta attttgtttc
    12361 aagtattaaa ggagtgcttg ctgaaatttc ttggattcgt ggatattgtt cgctatcttt
    12421 tccttttagg gtaatttctg atttgccact ggttaaaaca atttgatttt gttcaatttc
    12481 tttaaaatca agagttacat caggtaaact agatactaca ttgataaaga aagaagcttc
    12541 aagaaggatc gaacctaaag aagtaattaa caaaccagca tcttcatttt tttgagaaat
    12601 aaaattttca attgaaattt gaccatttga accaattaaa gtaatacctt cattggtcac
    12661 gtcaattttt actgttgata aaataggaat ggcattttta gaactaatag ctctcttagt
    12721 agtatttaat gcttgtagaa ataaattttt attaattgaa aaatgaatca tggattctcc
    12781 tttatttatt tttagtatta gaaagataat agtaataata gagtctgtga aatctgtgga
    12841 aaacagcctt taaacaagtc atatcaagtt ttttagcttg tttaaaaaat gtggataaaa
    12901 aagataaaaa actaaaagtt atccacaaat tatttgattt tctttttgat tgattcaatt
    12961 tctaaacgta aattatcgtc ttgatcaatc aaagatttta ttttggcatg ggcatgaatg
    13021 actgtggtat gatcttttcc cccaaattcc ttcccaattt ttggaagact attatctgtt
    13081 agttctctag ataaatacat ggctacttga cgggccaaaa caatattttg aaggcgtcta
    13141 cttcccttca tttctttgat actaacacca taaaagttac caacttcagt ttggatttta
    13201 tcaattggga tgacgagcat ttggctaaca tcttgtttgc gggctctaat ggcttctgca
    13261 gcaatatcaa tagtgatatc cttgattttt tttactctgg caattaaagt gatgtcgttg
    13321 atggctccct caagatctcg aacatttgaa tcaaattgcc cagctaggta ttctagagta
    13381 tcactttgga aattgtagcc taaatgttcc gttttacttt gtaaaatggc aatacgtgtt
    13441 tcaaagtcag ggggtgtgat agtttgtgtc aatccccaac taaaacgcgt gacaagcctc
    13501 tcctcgagcc cttctagatg ttttggacta cgatcactcg ttaggacaat ctgtttttgc
    13561 ttgtcatgaa gggcgttaaa ggtattgaaa aattcttcct gagttgcgac tttttttccg
    13621 ctgagtgact ggatatcatc gattaacaaa agatcaagac tacgataggt ctttttaaac
    13681 ttttccattt ccccaagtct taggtgatca agaaagtcat taataaagct ttcggcaggg
    13741 atatatttaa cacgcgcatt aggaatattt tttagaattt catttccaat agcgtttaat
    13801 aagtgagtct taccaaggcc tggtcctcca tagataaaaa gagggttata ggtcagagcc
    13861 aaatcttcag agacagctaa agcggctgac acagcccaaa catttccatc cccttgaata
    13921 aagttatcaa aggtatactt ttcttttaat cccgtatctg aataaggaat agatactaac
    13981 tttggactat agttataaag agttaaattt gtagcttctt caacttgtga gctagtcgta
    14041 tcttgaggtt tggtgaaaat atagtgggga gttatttcag cgtcataaat ttcaaaacca
    14101 gctactacaa taatatcttt tagttgtttt tcccagacca tttccatttc agagcgaggt
    14161 agaaatatag tggcaacatt ttcctctacc ttgatgagtt cagcttgaat agcatagaaa
    14221 tcatacatgg atcgagtcag tctttcttgt gcaaattcta atatacgatt ccaaaattgt
    14281 ttttctttca atcctttctc ctcctttact atttaaaagt ttaatactag agatatttta
    14341 ccatagatag caagaatttt ccacaagctg tgaaaaataa tccacaatgt taccaaactt
    14401 tatccacagg ttggggataa aagaagaaat tattgattta ttaagctttt tattgaaaaa
    14461 catagtggat aaccttgtga gataaaaaaa taaaagaaca gccttatttc aggctgttga
    14521 taattctact atattcttct tgatttgaaa aagaaataat gatttttcca ctgtcttttt
    14581 tagatagttt aatttctaca tctaatccga gtagttttct taactgtttt tcttcatttt
    14641 gtatgaaatg attagttttt tgctgtttct tttgtttttt ctctgtcaga agagcttcta
    14701 atttccttac agaaatatct tcttctataa tccgttgaaa gaaatagtct tgttgttcct
    14761 tatttaaccc aactagggaa cgcgcatggg cttgtgatag tttgccattt tctacttctg
    14821 aaagaatctg ttctggcaag gaaagtaaac gaatggagtt gctgatatat ggacgagact
    14881 tgcccatctt atctgcaatt tcagcatggg tgaatccttt ctctacgaga gattcatagg
    14941 cgcgtgcttc ttctattggg tttaaatttt ctctctgtaa attttcaata atggactgga
    15001 ccatcatctc ttggtctgaa atctgtttaa caacagctgg gatagaccgt agaccagcta
    15061 aaagtgaagc ccgatagcgt ctctctcctg caaggatttc ataaccaata acaggagatt
    15121 gacgaacaat aatcggttga atgaccccat tttctttgat agactgtgct agttcatcta
    15181 gtttttctct atcaaattct tttcggggtt gatagggatt tttttgtata tctgtgatag
    15241 aaatcatttc aaatttttcc atgattctac actaacacat cttttctctt atgtaaagct
    15301 ttctttacat agatgtcaat taagattcta aatcacctga actcttgtta agtttgatag
    15361 aggtagtttc ttctttcccg ttacgatagt aggttatctt aatggtgtct ccgatagaat
    15421 ggttgtaaag agcactttgt aagtctgttg atgaagcaat ctctttgtca tctacttttg
    15481 taattacatc gtatttttca aggtgaccat tggcaggcat attactttgt accgaacgaa
    15541 caactacacc agatgtaaca ttacttggaa tattgagtct tctgatgtcg cttgtactca
    15601 cattagataa attaaccatc tggattccca aagctggacg cgtcactttt ccgttttttt
    15661 ctaactgttc aataatattg atagcatcat ttgcaggaat tgcgaaacca agaccttcta
    15721 cagatgttcc tccatttgta gcaattttac ttgaggtaat tccgataacc tgcccttgaa
    15781 tattgatcag tgggccgcca gagttacctg ggttaatagc agtatcagtt tggatggctt
    15841 ttgtagaaat agcttgtcca tcttccgatt ttaaggatac atttctattg agactggata
    15901 cgataccttg agtgacagta tttgcatatt cagaacctaa cgggctaccg atggcaatag
    15961 cagtttctcc tacagttaac ttactagaat caccaaactc agctactgtt gtcacttttt
    16021 ctgaagagat ttttacgaca gcaatatcag agaaagtgtc agctccgaca atttctccag
    16081 gtactttagt cccatctgac aatcgaatat ctactttgct ggcgccattt ataacgtgat
    16141 tgttggtgac gatgtaagct tctttatcat tctttttata aataactcca gatccttcac
    16201 tagagattcg ctgagaatct gtgtcagtat catcattgcc aaatacgcta ttttgtctgt
    16261 ttgccgaata agtaataaca gaaacaacag catcttttac tttgttaaca gcctgtgttg
    16321 ttgaattttc gttcttatag gcagtttgtg taatagtact attgttgtta gagttgttta
    16381 cactactttt ttgagttagt tgagttattg aaaaactacc caaggctcca ctaaaaaagc
    16441 taatgacgat aacgactaat aattgaaacc attttttgta aaatgttttt agatgtttca
    16501 tatttgcctc catatgtttg aattactgaa agtataaact gactagctta attataactt
    16561 aaacacaaaa gttttacaca aactgtggat aactcttttg aaactgtgat tttcttaatt
    16621 gaaatctatt ttttattttg tgaataagat gtgaaaaaat agagaatatg ttagaataga
    16681 gtcatgaaaa ttaaagttgt aacagttggg aaactgaaag aaaagtattt aaaagatggt
    16741 atcgcagagt attcaaaacg aatttctaga tttgctaagt ttgaaatgat tgagttatca
    16801 gatgaaaaaa caccagataa ggccagtgaa tcagaaaatc aaaagatttt agaaatagaa
    16861 ggtcagagaa ttttatcaaa aattgctgac cgtgatttcg ttattgtgtt agccattgaa
    16921 gggaaaactt tcttctcaga agaatttagt aagcagttag aagaaacttc tataaaagga
    16981 ttttctactc ttacttttat tattggggga agtttaggat tgtcatcatc tgtaaaaaat
    17041 agagccaatc tttctgtcag ttttggtcgc ctaaccttac cccatcagtt aatgagacta
    17101 gttcttgttg aacaaatcta tcgcgctttt acgattcagc agggattccc ctaccataaa
    17161 tagaaaattg acttttaatt gaatttttgg tagaataatt gtattaggtc tcatagctca
    17221 gctggataga gcattcgcct tctaagcgaa cggtcgcagg ttcgaatcct gctgggatca
    17281 atataatagc aaagctggga attttcccgg cttttttctt aaaaaagtac actttgggag
    17341 aaaaaaatga cagttgagag aattttatct aaaacgaaat tccattttgt ataatggttt
    17401 ttgtaagtta gcttacaaga aaaaacattt taggagattt tattatgaaa aacacagtta
    17461 aattggaaca gtttgtagct ttgaaggaaa aagacttaca aaagattaaa ggtggggaga
    17521 tgaggttgtc aaaattcttc cgtgatttta ttttacaaag aaaaaagtaa tgaaataagg
    17581 ggaaagagta atggatttat ttggatttgg gacggttatt gttcattttt taattattag
    17641 tcacagttac cattttattt gtaaaggtca aataaataga aaagaattat tcgtttttgg
    17701 tgcttataca ttactaacag aaatagtatt tgattttccc ttatatattc tatatttaga
    17761 tgggttaggg attgaaagat ttttatttcc tttgggatta tattcctatt ttcgatggat
    17821 gaaacagtat gagagggata gaggactatt cctaagttta ctactatctc ttttatatga
    17881 gagcactcat aactttctgt ccgtaacttt ctcctctata acaggagata attttgtttt
    17941 acaatatcat ttcccattct ttttcgttgt gacggtgtta acctattttg ttacattaaa
    18001 aatcatttac tatttccatt tggaactagc ctattttgac gaggactacc tttatccttt
    18061 cttgaaaaaa gtattttttg ctttactatt gctacatatt gtatctttcg tttcagatat
    18121 ggtaagtacg attaaacatt tgaatagttt tggaagtatt ttgtcatcta ttgtctttat
    18181 ctctctcctt ttgaccttct ttgcaatgaa ttctcataaa gttcaaatgg agaaagagat
    18241 tgctttgaag cagaagaaat ttgaacagaa acatttacag aattacacag atgaaattgt
    18301 tggtctgtat aatgaaatcc gtggttttcg acatgattat gctggaatgc ttgtcagcat
    18361 gcagatggca attgacagtg gtaatttaca ggaaattgac agaatttaca atgaagtttt
    18421 agtcaaagca aatcataaat tgcgttcaga taagtacact tactttgatt tgaacaacat
    18481 agaagactca gctttacgaa gtttggttgc tcagtcaatt gtctatgctc gaaataatgg
    18541 tgtagagttt acactggaag taaaagatac gattaccaag cttccaattg aactattgga
    18601 tttggttcgt atcatgagcg ttttattgaa taatgctgtc gaaggatcgg ctgatagcta
    18661 taaaaagcag atggaagtag cagttattaa gatggaaact gaaacagtta ttgtgattca
    18721 gaattcatgt aaaatgacga tgactccttc aggagatcta tttgccttag gattctccac
    18781 taagggaaga aatcgcggag tcggattaaa taatgtgaaa gaactactag ataagtacaa
    18841 caatattatt ttagaaacag agatggaagg cagtacattt agacaaatca ttagatttaa
    18901 gagggaattt gaatgaaagt tttaatttta gaagatgtta ttgaacatca agtgagacta
    18961 gagagaatat tggatgaaat ttcgaaagaa tcgaatattc caatatcata caagacaacg
    19021 ggaaaagtcc gtgaatttga agaatacatt gaaaatgatg aagtaaatca gctttatttc
    19081 ctagatatcg atattcatgg aattgagaaa aagggatttg aagtggctca gctcattcgt
    19141 cattacaatc cttacgctat tatcgtcttt atcactagtc gatcagagtt tgcgactcta
    19201 acctataaat accaggtatc agccctagat tttgttgata aggatatcaa tgatgagatg
    19261 tttaagaaga gaattgagca aaatatcttc tacacgaaga gtatgttact tgaaaatgaa
    19321 gatgttgtag attatttcga ctacaattac aagggaaatg atttaaaaat tccttaccat
    19381 gatattttgt atattgaaac aacaggggtc tctcataaat tgcgcattat tggtaagaat
    19441 tttgcaaaag agttttatgg taccatgaca gatattcagg aaaaggacaa acatactcag
    19501 cgattttatt ctcctcacaa gtcatttttg gtaaatatag gcaatatcag agaaattgat
    19561 cgaaaaaact tagaaattgt tttctatgaa gaccatcgtt gtcctatttc aagattaaaa
    19621 attagaaaat taaaagatat tttagagaaa aaatctcaaa agtgattgac aattagcaag
    19681 aaattgatat aatggttata tctgctacag atagaaggtc cgttggtcaa ggggttaaga
    19741 caccgccttt tcacggcggt aacacgggtt cgaatcccgt acggactatt ttatccgcca
    19801 tagctcagtt ggtagtagcg catgactgtt aatcatgatg tcgtaggttc gagtcctact
    19861 ggcggagtat agataaaagg gaacacagct gtgttcctct ttttgtatca atttgtatca
    19921 ccaagcattt tcataaggaa gtctgttatt tcttgagaac tttctttttt tccatgtgca
    19981 atccaagttt ggcagacacc aaaaagtgca tgagttagat agatgctact atattctaat
    20041 tcggtggtat ttagattcag ttgcataaat cgcttttgta aatctgtact aagcatgata
    20101 tgaagtttat ttcgtaagaa attttggatt tctttagtcc cattttcaga aagaagggca
    20161 gccagaagtg gttctgactc tagatattca aaaacttcta aaatagcgtc tcttttgtga
    20221 tgagcatgtt tttgaaaaat atattcaaat gtatggaata gcttgctttg atagtgctca
    20281 atcatatcat acttatcctt atagtgagta tagaagctgg aacgactaat tccggctttt
    20341 tctactaatt tgacagtaga aattttatca aatggctgtt ccatcagtaa ttgtaccata
    20401 gcattttcaa tagttcgctt tgtttttaag cgtttgttac tttcttgcat atttcctcct
    20461 tgtaaacaaa ttagactata tgtttaaaaa tagatttttt atcttgtaat ttagattttt
    20521 taatgtataa tctattatat caaaatttta gacaatatgt ttaaaaaagg agaaactaag
    20581 tttaaagaat ggaaagcaat ttaaaaaaaa ccaaccttta ttattgtcat gatcgggatt
    20641 tctcttattc cagatctgta caatatcata tttttgtcat caatgtggga tccatatggg
    20701 caattgtctg acttacctgt ggcagttgta aataatgata aagaggcttc ctataatggt
    20761 aatactatgg caataggaaa agacatggtg tccaatttaa aagaaaataa aaccttggat
    20821 tttcattttg tagatgaaga ggaaggaaag aagggattgg aagatggcga ttactatatg
    20881 gtagtgactt taccaagtga tttatctgaa aaaacaacta cattatccaa tattcaatcg
    20941 acagcagctt atcaatcatt gacaagtgag caacaaactg agataagtga ttctgtatct
    21001 caaaattcaa ctgatagtat tcaatcggct cagtcaattg tagctttagt acaagattta
    21061 cagggaagtt tagaaaactt acaaaatcaa tcttctaatc tttcgacttt aaaaaatcaa
    21121 tctaatcaag tatcacctat tacttctact tctttgatag gattgtcaag tggattaaca
    21181 gagatacaag gagatgttac tagcaaatta gttcctgcca gtcagtcgat tgcatcaggt
    21241 gtaaacgcat atactacagg tgttgataaa gtttctcagg gcgcaagtca actaagtgaa
    21301 aaaaatgcca ccttgacagg tagtttggat aaactagttt caggctcaaa caccttgaca
    21361 caaaaatctt ctagattgac agcaggagtt ggttaattac aatcaggatc tgggcaatta
    21421 gcagacaaat ccagtcagtt actttcaggt gcttctccat tagagaatag agctaataaa
    21481 ttggcagatg gatctgggaa actagcagaa ggtggaacaa agttaacttc tggattggaa
    21541 gatttacaga caggacttgc ttctttagga caaggactag gtaatgctag tgatcaactc
    21601 aaatcagtat caacagaatc taaaaatgca gagattttgt caaatccact caatctttca
    21661 aaaacagaca atgatcaagt tcctgtaaat ggaatcgcaa tagctcctta tatgatatca
    21721 gttgctcttt ttgttgcagc aatatcaaca aatatgatat ttgcgaaatt gccttcagga
    21781 cgtcatccag agagccgttg ggcttggttg aaatctcgag ctgaaataaa tggtattata
    21841 gctgttttgg caggaatttt ggtatatgga ggagttcagc ttattggttt aactgctaat
    21901 catgagatga gaatatttat tctcatcatc ctaacaagtt tagtattcat gtctatggtg
    21961 accactttaa caacgtggaa tagccgtata ggagcttttt tcttacttat tttgctttta
    22021 ctacagttag catcaagtgc aggtacttat ccacttgctt tgacaaatga tttctttaga
    22081 tctattaatc cctggttacc aatgagctat tcagtttcgg gattacgaca aacaatctct
    22141 atcaacaagt cattttccta gctgtcatac tagctctatt tactggttta ggtatgctag
    22201 cctatcaaca taagaaaatg gaagaagatt aaaaaaatcg accgattaat gaactgcacc
    22261 ccaaaagtta gacagaaaaa atctaacttt tgaggtgttt tattatgaaa ttaacttatg
    22321 atgataaagt tcagatctat gaacttagaa aacaag
//
LOCUS       DAVBCV010000032        22104 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBCV010000032 DAVBCV010000000
VERSION     DAVBCV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.032"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..389)
                     /locus_tag="VQ516_001234"
     CDS             complement(<1..389)
                     /locus_tag="VQ516_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2511677.1"
                     /translation="MKTFFAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGL"
     gene            complement(386..550)
                     /locus_tag="VQ516_001235"
     CDS             complement(386..550)
                     /locus_tag="VQ516_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511678.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            complement(849..968)
                     /locus_tag="VQ516_001236"
     CDS             complement(849..968)
                     /locus_tag="VQ516_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2511679.1"
                     /translation="MDFKSFIIGLVVGIFGPYMYNLIRKIFSKFPKKDTDSTP"
     gene            complement(971..1201)
                     /gene="blpU"
                     /locus_tag="VQ516_001237"
     CDS             complement(971..1201)
                     /gene="blpU"
                     /locus_tag="VQ516_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2511680.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(1270..1539)
                     /gene="blpJ"
                     /locus_tag="VQ516_001238"
     CDS             complement(1270..1539)
                     /gene="blpJ"
                     /locus_tag="VQ516_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET2511681.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(2006..2203)
                     /gene="blpI"
                     /locus_tag="VQ516_001239"
     CDS             complement(2006..2203)
                     /gene="blpI"
                     /locus_tag="VQ516_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET2511682.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            2485..3072
                     /locus_tag="VQ516_001240"
     CDS             2485..3072
                     /locus_tag="VQ516_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2511683.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            3065..4654
                     /locus_tag="VQ516_001241"
                     /pseudo
     CDS             3065..4654
                     /locus_tag="VQ516_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
     gene            4665..6026
                     /locus_tag="VQ516_001242"
     CDS             4665..6026
                     /locus_tag="VQ516_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2511684.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMDESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIASLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLLLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            6083..6238
                     /gene="blpC"
                     /locus_tag="VQ516_001243"
     CDS             6083..6238
                     /gene="blpC"
                     /locus_tag="VQ516_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2511685.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(6282..7622)
                     /locus_tag="VQ516_001244"
     CDS             complement(6282..7622)
                     /locus_tag="VQ516_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2511686.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNTYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(7636..8373)
                     /locus_tag="VQ516_001245"
     CDS             complement(7636..8373)
                     /locus_tag="VQ516_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2511687.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(8378..8716)
                     /locus_tag="VQ516_001246"
     CDS             complement(8378..8716)
                     /locus_tag="VQ516_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2511688.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            8993..9322
                     /locus_tag="VQ516_001247"
     CDS             8993..9322
                     /locus_tag="VQ516_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2511689.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(9490..10539)
                     /locus_tag="VQ516_001248"
     CDS             complement(9490..10539)
                     /locus_tag="VQ516_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2511690.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEAIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(10536..11267)
                     /locus_tag="VQ516_001249"
     CDS             complement(10536..11267)
                     /locus_tag="VQ516_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511691.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            11335..11745
                     /locus_tag="VQ516_001250"
     CDS             11335..11745
                     /locus_tag="VQ516_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2511692.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            11755..12042
                     /locus_tag="VQ516_001251"
     CDS             11755..12042
                     /locus_tag="VQ516_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511693.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            12204..12443
                     /locus_tag="VQ516_001252"
     CDS             12204..12443
                     /locus_tag="VQ516_001252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511694.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(12793..13929)
                     /gene="dnaJ"
                     /locus_tag="VQ516_001253"
     CDS             complement(12793..13929)
                     /gene="dnaJ"
                     /locus_tag="VQ516_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2511695.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(14134..14487)
                     /locus_tag="VQ516_001254"
     CDS             complement(14134..14487)
                     /locus_tag="VQ516_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511696.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(14500..16323)
                     /gene="dnaK"
                     /locus_tag="VQ516_001255"
     CDS             complement(14500..16323)
                     /gene="dnaK"
                     /locus_tag="VQ516_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2511697.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(16803..17327)
                     /gene="grpE"
                     /locus_tag="VQ516_001256"
     CDS             complement(16803..17327)
                     /gene="grpE"
                     /locus_tag="VQ516_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2511698.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(17354..18388)
                     /gene="hrcA"
                     /locus_tag="VQ516_001257"
     CDS             complement(17354..18388)
                     /gene="hrcA"
                     /locus_tag="VQ516_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2511699.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTGLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(18552..19061)
                     /locus_tag="VQ516_001258"
     CDS             complement(18552..19061)
                     /locus_tag="VQ516_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2511700.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            19669..21606
                     /locus_tag="VQ516_001259"
                     /pseudo
     CDS             19669..21606
                     /locus_tag="VQ516_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            21604..22104
                     /locus_tag="VQ516_001260"
                     /pseudo
     CDS             21604..22104
                     /locus_tag="VQ516_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         6993 a         4580 c         3952 g         6579 t
ORIGIN      
        1 ttcaagcccc acatgagcag aagcatgatg attaagcaga gaacagcgcc aatataagcg
       61 attatttgtt ggtaggattc tcctgctgtg atacctctat acaaacaaat aatagacata
      121 aaacctgtca agccgatgaa cataagttga ttggttctag gactaaccaa atcatcatct
      181 tcaaactctc ttatcctcat ttccctagtg agataaacag taaccaaaat agaagccaag
      241 ttaataacta ctaaaagaaa ttggaaaact acggaaaaat ttaaaaactg acgagataga
      301 aatagataag tagaaacaag caagggcaac tgacctaaga acaatctcgc aaggaagatg
      361 ttccgttttt tagcaaaaaa agttttcatt tcttttctcc tttcttttta ttgatagcaa
      421 aatagatcat aactgcaatc acataggcta tggtataaaa tagctgatac caagcactct
      481 ccctaagcgg atatagaaag atggacatga ttagatacag aacgaaaata atcagtattt
      541 ttttcttcat aagatttcct cctaaatgtg cgatttatct tagttgagca agaacattta
      601 cactgctagt atagcactta ttttgacctt ggatcactca aatcataaat ggtcatcgaa
      661 acatcttgaa ttgttaaaaa atttaaaaag taagcattaa aaacatactt tcctctttat
      721 attgtattga taccaacttg tttgtagact tttcatcctg ctatcacata tcattttgac
      781 aggcgaaaca aaattaaaga aactcccctg taaattaagc tagcaaatac aggggagaaa
      841 tttatttttt agggggtact atctgtatcc tttttgggaa attttgaaaa tatttttcta
      901 attaaattat acatataagg accaaatata ccaactacta aaccaataat aaaactttta
      961 aaatccataa ttaccaccaa catgttgctg cataggctac acctccaagt atagctccac
     1021 ccgcagcacc agttgctgca ccttgccatg ttcctgtttt aatgcctagt tgaagacctc
     1081 ttgctgctgc tcctccaaca cctgctttgg caaaatctcc ccaattgcat ccgccacctt
     1141 caacgcaagc aagcatttca gtatccataa cagaaaattg tgacatcatt tttgtatcca
     1201 tgacaaatac tcctttttta aaaaactaaa ataaatcaga atagaatcct cataatttta
     1261 ctataagtct taccaactta gtcccaattt atcaccaacc atacctccta agcatgttaa
     1321 tccaccccca attgcaccaa tgtgtgctcc aacaaatgca ccagcaagtc cagctactcc
     1381 taaagtggcc aaacctgctc cagttccacc agttataatt cccgtagtga ctcctgtaat
     1441 cagtgcattt tgacaatcag tggagctata ccccccttca actttcgcaa gcatttcagt
     1501 atccataacc tctaactgtg acaacatttt tgtattcatg atgaatacct cctttttatt
     1561 ttcaatttgt taccaaagtc ttaaattcaa taaacaaata gattttttat agtatctttt
     1621 tgattttctt aaaaaagtat atacgtctac tatcttctta aaggtagcag tacctatttt
     1681 ttagtctaag atttcaataa tcttgagtat ctaaaatatc ttaatttcgt tattctcctt
     1741 gcaataaaaa gttttactat actatttatt aacttgcaga aagcaaaaaa tattagtaaa
     1801 taatagttta tagttaagtt ttttattcct accaatccat caactaagta aagcatcaac
     1861 gattacataa acgattgata atataattaa aattttgcta actatcttat tctcatcatt
     1921 cttagataac tttgatattt tgtaagtaag taaataagac agtaaattaa tagcgataat
     1981 aatactatat ttaagaatca taatcttaca aagaggacat aattcctgaa cctacacaaa
     2041 taagtgttgc tgctccccca gttatcggac cagtcgcagc agctaatagt actgctccaa
     2101 tacaaccacc gattgcagat cctaaattgc ctcttcctcc actaactatt tcgagttctt
     2161 cattatccat aacagaaaat tgttccatca tttttgtatt catgacaaat actccttttt
     2221 tcttttttta tttttgtctt gttgtaactt tgataagttt agtatatcat cgttttttaa
     2281 aatttttcat ccagatcttg aattgtcatc gaaacgtctt gaattagctt ttttatttca
     2341 agccacctct aaatgtttaa aaaaaataat ttctaatcac ttttttacca ttcaggaagt
     2401 tttaatgact attcaagatt tcataaaata tgaacttagt tttatgacat aatagaccta
     2461 tccactatat gaaaagaatt gccaatgact tcttataaac gtacatttgt tcctcaaata
     2521 gatgcgagag actgtggtgt cgctgcctta gcctcgattg ctaaattcta tggttcagat
     2581 ttttctctag ctcacttgag agaacttgca aagaccaata aagaagggac gactgctctt
     2641 ggcattgtaa aagccgctga tgaaatgggc tttgaaacaa gacctgttca agcagataaa
     2701 acgctctttg acatgagtga tgtcccctat ccatttatcg ttcacgttaa caaagaagga
     2761 aaactccaac attactatgt tgtctatcaa acaaagaaag actatctgat tattggtgat
     2821 cctgaccctt ctgtaaaaat cactaaaatg tcaaaagaac gctttttcta tgaatggact
     2881 ggagtagcta tttttctagc taccaaaccc agctatcaac cccataaaga taaaaagaat
     2941 ggtctactaa gcaagcttcc ttcctctgat tttcaaacaa aaatctctca ttgcttacat
     3001 tgttctctca agcttattgg tcactattat caatataggt ggttcttact atctccaagg
     3061 aatcttggat gaatacattc caaatcagat gaaatcaact ttaggaatca tctcagttgg
     3121 tctggttatc acctaggtta tcacctatat cctccaacaa gtcatgagct tctccagaga
     3181 ttatctccta accgttctga gccagagatt aagcattgat gtgattttat cctatattcg
     3241 ccatattttt gaacttccca tgtctttctt tgcgacacgt cgtacaggag aaatcatttc
     3301 acggttcaca gatgctaact ctattataga tgccttggct tctaccattc tttctctttt
     3361 tctggatgtt tctattctga ttcttgtagg gggagtcttg ctggcacaaa accctaatct
     3421 cttcctcctt tctcttcttt ccattcctat atacatgttc atcatctttt cttttatgaa
     3481 accttttgaa aaaatgaacc atgatgtcat gcaaagtaat tctatggtta gctctgccat
     3541 tatcgaagat atcaacggga ttgaaactat aaagtcgctc acgagtgaag aaaatcgcta
     3601 tcaaaatata gacagcgaat ttgtagatta tttggaaaaa tcctttaagc tcagtaaata
     3661 ttctatttta caaacgagtt taaagcaggg aacaaaatta gttctgaata tccttatcct
     3721 atggtttggc gctcaattag tcatgtcgag taaaatttct atcggtcagc tgattacctt
     3781 taacacactt ttttcttact ttacaactcc tatggaaaat attatcaacc tccaaaccaa
     3841 actcccatct gcgaaggtcg ctaataaccg tttgaacgaa gtctatctag tcgaatctga
     3901 atttcaagtt caagaaaacc ctgtccattc acattttttg atgggcgata ttgaatttga
     3961 tgacctttct tataagtatg gttttggacg agatacctta acagatatta atctcacgat
     4021 taaacaagga gataaggtta gcctagttgg agttagtggt tctggtaaaa caactttagc
     4081 caaaatgatt gtcaatttct ttgaacccta caaagggcat atttccatca atcatcagga
     4141 tattaaaaac attgataaaa aagtcttgcg ccgtcatatt aattacctac cccaacaagc
     4201 ctatatcttt aatggctcta tcttggaaaa tttaaccttg ggcggtaatc atatgattag
     4261 ccaagaagat attctaagag cttgtgaatt agctgaaatc cgtcaagaca ttgaaagaat
     4321 gcctatgggc tatcaaactc agctctctga tggagctggt ctatcaggag gacagaagca
     4381 acgaatagcc ctcgctcgtg ctcttttaac taaatctcct gttttaatac tagacgaagc
     4441 tactagcggt cttgatgtct tgactgagaa aaaagttata gataatctta tgtccctaac
     4501 tgataaaacc attctctttg tagcccatcg tctcagtata gccgaacgaa ctaaccgtgt
     4561 cattgttctt gaccagggga aaatcattga agttggtagt caccaagagt taatgcaggc
     4621 gcaaggcttc taccatcatc tgttcaataa ataaggagaa tgtcatgaat cctaatcttt
     4681 ttagaagcgt cgagttttat cagagacgtt accataacta tgcgacagtg ttaattatac
     4741 ctctttcatt actgtttact ttcatcttga ttttctccct tgttgccaca aaagaaatta
     4801 ctgttacttc ccaaggagaa atcgccccta caagtgtcat tgcatccatt cagtcaacca
     4861 gtgataatcc tatccttgct aatcatttag tggcaaatca agtagttgaa aaaggtgact
     4921 tactcatcaa atactctgaa acaatggatg agagtcagaa aactgcctta gaaactcaat
     4981 tacaaagact tgagaagcaa aaagaaggac ttggaatttt gaaacaaagt ttagaaaaag
     5041 cgactgatct tttttctggc gaggatgagt ttggctacca taataccttt atgaatttta
     5101 ctaaacaatc ccatgatatt gaactgggta tcacaaagac taacaccgaa gtttcaaatc
     5161 aagctaatct tgccaatagc agttcatcag ccatcgaaca agaaattaca aaagttcaac
     5221 aacaaattgg agaatatcaa gagttgagag atgctatcat aaataacaga gcacgcttac
     5281 caactggcaa tccgcaccag tcaattttaa atcgttatct tatagcctct caaggacaaa
     5341 cacaaggaac ggcagaggag ccatttttat ctcaaattaa tcaaagtatt gcaagtcttg
     5401 aatcatctat cgcaagcctc aaaattcagc aagctggtat cggaagtgta gcaacttatg
     5461 ataatagttt agcaaccaaa attgaagtac tccgcactca gtttttacaa acagcctcac
     5521 agcaacaact aaccgtggag aatcaattaa cagaattaaa agtacaacta gatcaagcga
     5581 cacagcgctt agaaaataat accttaaccg ctccaagtaa aggtatcgtt catctgaaca
     5641 gcgaatttga aggtaaaaat agaattccaa ctggtacaga aattgctcaa atattccctg
     5701 tcatcacaga tacaagagaa gtactaatca cttactacgt atcatctgac tatctcctct
     5761 tactagataa aggacaaact gtaagattaa aactggagaa gattggaaat cacggcacca
     5821 ccatcatcgg ccaacttcag agtattgatc aaactcctac cagaacagag caaggcaatc
     5881 tctttaaatt aaccgctctt gcaaaactat ctaacgagga tagtaaactc atccaatatg
     5941 gcttacaagg tcgcgtcact agtgtaacta caaagaaaac atattttgat tatttcaaag
     6001 ataaaatttt aacacattct gattaatttt cagataacac tctataacta tttattatct
     6061 tatcaaaaag gagaatcata acatggataa gaaacaaaac ctaacttcat ttcaagaact
     6121 aacaactacc gaactcaacc aaattatagg tggaggatgg tgggaagatt tcttatatag
     6181 atttaatata attgaacaaa aaaatacaaa aggatttcat cagccaatac aactataaaa
     6241 aaacaagacc aagagaacgc actctcggtc ttattttttc ttcattctat atgtattaca
     6301 gtaagtacct gacgaaaaac gtgatcttgg caggtggtat tgagactggt attgggatga
     6361 ctttccacaa ttttcataac ggtatagaga ccaactcctc tctcctcccc tttagaactt
     6421 gctccaaagg agaagatttc agaaatatcg attccctctt ctttgatgga attttcaatg
     6481 ataaaggtct cctgtgctcc attttttaaa aaggcaattg aaacatgagg ttgacaggct
     6541 tctacactag cttcaatagc gttgtcacaa aggatagaca caacggttag aaaatcaagt
     6601 agactcatcc cctctacctg aatctcctct ggaacttcga cattaaagac aatgttctta
     6661 tctctagctt ttagaaattt ccctgctagg agacttttga gggctttatc acgaatattc
     6721 accaatcgac caaggtcata tttattgtcc tgcaattttt cactagaatc ctttaagacc
     6781 gagtcgtaga tctcttttat ctgctccata tcctcctctt caatgcccag acgtaagcta
     6841 gtcaagaggt tggtgtagtc atggcgaaaa ctccgtactt ccttataaag ctcctctata
     6901 tgccgactat accgctccat ctctctgtag cgcaagtcct gctcttggtt cagtctctca
     6961 tggagtttgt ccttcaaata ggtatccaat ttcttgataa tccccataaa aaagagcagg
     7021 taaaagacta ggatgagatg gcgaacagtc gttgattgaa tcccctgttc atattcaaag
     7081 taagacagat tttgtatcac caaatagtaa gcccccatta tccagttaat ctgagtcagg
     7141 gacttttgaa aatctttatc gagaatcccc tttctcaagc tagtgaaatc atagtccaac
     7201 catttcaaaa aagctagaga aatgaagaaa ttgaaaatta ttatacataa ccaaataaat
     7261 gagtagtcat catatacttg cccttgtccc aaaaatggaa gcacaaaata ggaaacacct
     7321 ctataaaaga gattcactaa tatcattgga aagagaccat aaaagagaag gagtttttta
     7381 ggaagttctc ttaacaataa gaaagataag cctatgccgt acaagggttc cataaaataa
     7441 gatagataag tatttcctac catatagcca agcattccaa aaacaacggc caacagtatc
     7501 ttaaaaagaa aggccttaaa aatcctctca aaagtgagac taattccatc taccttaaag
     7561 aatatgacaa tttttaatcc gtgagtaaca agtgtataca acaatatcca agcaacattc
     7621 ataaactctc ctagctcagt gtaagttatt aatagcctca gacacttccc tgaccttata
     7681 acgcgcgatt agacaacttc caccattggg aaagaaaagc agtttttctt tcttatctaa
     7741 atgcaccaca ttggcaggat tgatgagaaa agagcgatgg cactgcaaga gacgaggctc
     7801 ctgcttgaga acctcctcta gactcgctgt aaattccagc ctgtctgtct tggtatagag
     7861 aataacacga tggggtcttg tggacgtttc gagatagtaa acctctttaa aaggatactg
     7921 gaattgagca aattttgatt taaagtaaaa gcaatcttcc gctagacttt tactgtcttg
     7981 accattggca tagaggaggg ctgtctcgat acgagattca aactcctctg ccgaaagggc
     8041 cttatcaata tagtccagag cagacacttg gtagcgaaag gacaggggca taaactccga
     8101 gtgagtcgtc acaaagacaa tcagggcata cggatcccga tcccgaatct ttctagccac
     8161 ttccagaccc ttcatctcct catttcgaat ctcaatatcc aaaaagaata gctgatgggc
     8221 ccccttctca tgcacctctg ccagcagttg gtctggcttg ccaaatacct caaaagagct
     8281 gggagtgata tgatgttcct tcaagagttt ctcaatcgtc gtttcaatcc tagtctgttg
     8341 ggaaaaatca tcctctaaaa caaatattct catcttctta ctctccctct ttcaaccatt
     8401 tttgacgaat ttctctatag cgacgtctgg catacttgac agcgtattga ccttcttctc
     8461 cgagaaaaag gattctttct tgaaaatcaa tcgattttaa tttatcaaga ttaaccaaac
     8521 aatttcgatg acatctcatc aaggtttccc catattggtt ctcaagatta cttaaatttt
     8581 gaagcatatt aaaactagct tcttctgtaa caatctgtac ggtatgggct ttagttggat
     8641 gtgtttggat atagtagata tcgtcgacgt ttactttata gactgttttc tgcgtctgaa
     8701 taatcatatg tttcattgga ttctcctcct aagaaaagaa taacagaaaa gttctagccc
     8761 aagcccaccc aatcctaaac agtctttgaa agctcctaaa aggattattt ttctacaaca
     8821 tcatgatgag cttacaacta ttttatcata aaattcactc agcgcaattc aagacatttc
     8881 aatgacaatt aaagatttga ataaaaaaaa agaattaaaa atgataaact aacagtatca
     8941 aaacacagtt gccagaagta agactggcac ccagattaaa ggaggcattc ccatgacaga
     9001 tacagacccc atcaaaagag ctcatacttt gattactgac ttaaacaaag cctaccaagc
     9061 atgcaaacag gcaagcgctg acgacgtccg ctttcaggag caattaaact ctattcttgg
     9121 ttttttagcc aaggctgaaa cagtggataa tcgattcttg attgaactag aaaaatttta
     9181 ccagacttcc agtcttctca tgggactcag cgctcttgat ccagatgctc caactcgcgc
     9241 cgcttggcgg gcctatgacc gcttccactt tgaccaagtc aagaccaagt taatactcaa
     9301 tgaaaatcaa agagcaaact aggaagctag ccgcaagctg tacttgagta cggtaaggcg
     9361 acgctgacgt ggtttgaaga gattttcgaa gagtataagt ctctacggac caaccattat
     9421 cctgtagtaa caaaaaagaa gctgagacag actgaactca acttcttttt tagtgtcgta
     9481 tcagcaatgt tagtcctgca tctgacgttt tacctgataa ggcaaataca agactagtaa
     9541 aaccaaacca gctcctagta aggctagaag ggccagtttt tcttggaagg taatcaaccc
     9601 aacaactaat tccacaagta aaacaaaact ggtcaatcct cgaactacct cctgtaagcc
     9661 tttttccttt tgccccttgt ccagcggaaa gagttgggtc aaatactggt agtcaaaggc
     9721 atgatagagg gccagcaact ggaagagcaa gaggtagtta aagagaacta ccactgctgt
     9781 cgcaatccaa gcttgctcga taaaaacctg cgccagtacg gaaagtaata gaagacgaag
     9841 actgagagca aagaggtcgc catttcgcag ataagaacgc agatagagat tttgccaaat
     9901 cttcccaggc accttctgaa cagcctttaa gataaagtcc agataggcac gacgcttaac
     9961 gctgtttgaa attcccttga cctgcgtaaa gagggcaaag aaacgaagca agacttgctt
    10021 acgcttgctt tcttgagaaa taacatagtc ccagtccagt ccagtttcag taaaaaattt
    10081 gctggccttt tgacgaaagt ggaaatattt tcctaccccc aataaaagca catatagcag
    10141 aaaaactggc aagccataac ccattgctaa aaataaaggc gcaaataaca gcaagaaaag
    10201 ggtctgtaca aagagccaaa agactaggga aatgccagtt tgacgcttga gatggagctt
    10261 gattgcctct tctccaacta agagaaagag cttgtctgga gcctccatat aggtggcaat
    10321 tcctccccaa agtaagagta aaacagacgt aattcctaca aacaaaagga taggccaatg
    10381 attttcagga aaatgttgta agagttgact gtactggtag gctagaaagc ccaacaggac
    10441 aagcaggaac aagacaaagt ggtcattgag cacatagcgc agataacaaa gacactcctt
    10501 acgaaaggcc tgctttctct ttaaaaacaa gtctttcata gatcctcctc tttggtcaga
    10561 gccaagtaaa tatcattcaa actagcctca ggcatatcaa aggcttcacg tagttgcagg
    10621 agattgcctt tggcacgcac ctctcccttg tgaagaatga caaaggcatc acacatcttt
    10681 tccgccgaat ccagcacgtg ggtactcatg agaatagact tgcccttttg cttctccact
    10741 tccaaaagct gaatcaaatc agaaatagcc agcggatcaa gaccaaggaa aggctcatcc
    10801 acgatgaaaa gacttggatc caccacaaaa gcacagataa tcatgacctt ctgcttcatc
    10861 ccttttgaaa aatgaacagg gaaccagtct aatttctgtt ccaaacggaa catttttaac
    10921 aagggctcta ctcgttcgaa agccactttt tgctcaatac cgtaagccat agcaaccgtt
    10981 tcgatatgct ctctgagggt caattcctca tacagactag gcgtctcagg aatgtagcca
    11041 atctgcttgc ggtagctagt cgcatctcct tgcagagtca ggccattgat attgatggag
    11101 ccactataag gtgccaacag accgataatc tcattgatcg tcgttgattt cccagcacca
    11161 ttgagaccaa tcaaaccgac caactgccca ctttcaacag taaaggacac atctttcaaa
    11221 acaggaacat gaacatagcc acctgtcagg tttttaattt ctaacatatt ttctccaaat
    11281 ctggtataat gtagctatat tatatcaaaa ttcagtacag tagaggtaga ttttatgtca
    11341 gattgcattt tttgtaaaat catcgcaggg gaaattcctg cttcaaaagt atatgaagat
    11401 gagcaggtcc ttgcctttct tgatatctct caagtaacac taggacacac cttggtcgtg
    11461 ccaaaagaac actatcgcaa tcttttggag atgaatgcta cgagcgccag ccaactcttt
    11521 gcccaagtac caaaagtagc tcaaaaagtc atgaaagtca ctaaggctgc tggtatgaat
    11581 atcatttcca actgtgaaga agtcgctggt caaacagttt ttcatactca cgttcacctt
    11641 gtgcctcgct acagtgctga cgatgacctc aagattgatt ttatcgccca cgaaccagac
    11701 tttgacaaac ttgctcaagt cgctgaaacc atcaaaaacg cttaaggagt tgctatgaaa
    11761 ttatccaacc tactgctatt tgcaggagct gcagccggaa gttatctggt tacaaaaaat
    11821 cgccaaacca tcacagatga agtcttgaat accactgacc gcgttcaagc tatcaaagac
    11881 gatgtggata ttatccaaaa tagcctgcaa atcatcgacc agcaaaaaga acttatcaag
    11941 gaataccaag aagacttgac ttacaagttt aaggtcttgg aaaaggatat ccagactaga
    12001 ctagctgtga taaaagaaat gcagggaact gaagataagt aaaaagagcc ccccggctct
    12061 tttttaaaac gtaaacaagt tatagtgaat tgaatttaga atagcacatc atagtttcta
    12121 aagcattgtt agagattact ttaaaatcct cttatcaatt tgttcatatt ctatttcaat
    12181 ctactatata tatcccatca actatgcatc ataatttagg taactcatac tcaataaaaa
    12241 tcaaaaagca aactagaaag ctaggcacag actgctcaaa acaccgtttt aaggttgtgg
    12301 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
    12361 tcagtaacca tacccatgac aagccgacgc tgacgtggtt tgaagaaatt tttgaagagt
    12421 atcattcacc atttcacaac taaatcgaag actttctgcc gtaatgaaaa cacctgaaac
    12481 agcttggttt cagctgtccg gaaactttaa gacttaggtt caaagtttag gtatggaact
    12541 tcgaaggagg tcgctaccgt ccgtcattac ttaggaaagt cttaaaaaat ctataaagta
    12601 aaaagagccc gacagctctt tttgtttata ctcaatgaaa atcaaaaagc aaactaggaa
    12661 actagccacg ggctgctcaa aacactgttt tgaggttgta gatacgactc acatatatac
    12721 ggcaagccga cgctgacgtg gtttgaagag attttcgacg ctgacgtggt ttgaagagat
    12781 tttcaaagag tattattctc catcaaaggc atctttaata tggtcaaaga agcctttttt
    12841 ctttggattt actttcaagt caccagcagc cgcgaattct ttcaaggcta ctttttggcg
    12901 gtcgttcaag cctgtcggtg ttacgacatt aacagtaacg tattggtcac caactgcacc
    12961 gccacgaagg ctcggtgccc ccttactacg taggcggaat ttcttaccag tctgagttcc
    13021 ctctggaata accaattcaa catcaccgtg aacagttgga atatctactg tatcaccaag
    13081 agccgcttgg acaaagttga ggttgagatt gtagaagata gtcgttcctt cacgttcaaa
    13141 cttgtcgcta gcttctacag aaactactac atacaagtcc ccataaggtc caccgttaaa
    13201 gcctgcttcg ccttgaccag cgaggcgaat ttgttgacct gtttccacac cagcagggat
    13261 tttcacatgt acgctatgag cttgtttctc atgacctgtt ccatgacagg ttgtacatgg
    13321 atatttgatt tcttttcctc gaccgtgaca gacatcacag gttacttggc gacgcatcat
    13381 accaagagga gtctgcgtat cgacgttaat gacaccagcg ccatgacagc gtccacaagt
    13441 gactggactt gtccctggct tagcaccaga gccattacat gtacgacagc cagcttcacg
    13501 atgatactta acttccttct cagttccgaa gatagcttct tcaaaggtca aattgacacg
    13561 atactggaga tcatctcctt ggcgaggagc gtttggattg cgcgaagaac cgcctccgcc
    13621 gaagaaactt gaaaaaatat cctcaaaacc accgaagcca cctgccccat tgaaaccacc
    13681 gaaaccacca gctccaccaa aaccaccatt ggcgcctgca gcaccatact ggtcataggc
    13741 agcacgtttt tggtcgtcac tcaaagtctc ataggcttct tgaacttcct tgtacttgtc
    13801 ctcagcacca ggctccttgt tgatatctgg gtgatatttt ttggaaagct tacgataagc
    13861 ctttttgatc tcgtctgccg aagcgttttt tgacaccccc agacgatcat aaaattcagt
    13921 attgttcata caagatacca agaccgtaaa attcgactga aaaataggaa atctgacgct
    13981 ggagcgacgc tcctagacag atttctcttt tttccgagaa tttaggtcgg gttcaattcc
    14041 tttcttttat attgatgaga taatactcca gccactcggc cctatccctt tttaccgaaa
    14101 aacagaggct gagttgcaac ctctgtggtt agattattca tttgaaataa aaatggttgt
    14161 attatctcta gtagtaatct tcaaagacct tttagctaaa tcaaactcaa atttattata
    14221 gtgtgggtct tgtgttttat ataggtgtaa ctcctgacat atatgatccg aaaagtaaat
    14281 cataacatct ttaaacacag aagtgtggtc atatttactc ttaccaacag ctacttctgt
    14341 aaattcttta taaatgacat ttccatttct ttccacagtc accagcattt ctgttggatt
    14401 ataatccaaa ttaaataatt cttcaaaaaa ctttatcttt tcatgataat ctgcaatgtc
    14461 agaaagatta tttttattaa cactcataac actagcccct tatttttcag taaactctcc
    14521 gtctacgacg tcatcgcctg cgtttcctgt tgcttgtgcg ccttctgctc cttcttgagc
    14581 ttgttgcgct gctgcagctt gttcgtagag tttaacagca agtccttgag ctttttcgtt
    14641 caatgcttca agttttgctt tcatgtcgtc caagttgttg tcttcttgag ctttcttaag
    14701 gtcatcaagg gcagcttggg cagcgtcacg ttctgcgtcg aagcctttac cttcagtttc
    14761 cttgattgtc ttttcagtcg caaagattgc ttggtctact tcattacgaa ggtctacttc
    14821 ttctttacgt ttcttatcgg cttcagcgtt tgcttctgca tctttcatca tgcggtcgat
    14881 ttcttcgtca gtcaaacctg agttcgattg gatgacaata gtttgttctt tttgagttcc
    14941 aaggtctttg gccttaacag acacgatacc gttcttgtcg atgtcaaatg ttacttcgat
    15001 ttgaggaatt ccacgaggtg cagctgggat atcagtcaat tggaagcgtc caagagtctt
    15061 gttatctgct gccattgggc gttcaccttg aagaacgtgg atatcaacgg ctggttggtt
    15121 gtctgctgct gttgagaaga cttgtgattt agatgttggg attgtagtgt tgcgatcgat
    15181 aagttttgta aatactccac ccattgtttc gataccaagt gacaatggcg ttacatcaag
    15241 aaggacaacg tccttgacat caccagtaat cacaccacct tggatagccg cacccatagc
    15301 aactacttca tcagggttta ctgatttgtt tggttcttta ccagtttcag ctttaacagc
    15361 ttcaacaacg gcagggatac gagttgaacc accaacaagg ataacttcgt cgatttctga
    15421 caagctcaaa cctgcatctg aaagggcttg acgaactgga acttttgtac gttcaacaag
    15481 gtcacgagtc aaatcgtcaa atttcgcacg agtcaaagtc atttccaagt gaagaggtcc
    15541 agcctcacct gcagtgataa atggcaagct gatttgtgtt gaagttacac cagaaaggtc
    15601 tttcttcgct ttttcagccg catctttcaa acgttgcatt gccatcttgt cagtagacaa
    15661 gtcgataccg ttttctttct tgaattctgc taccaagtgg tcaatgattt tttggtcaaa
    15721 gtcgtcacca ccaagtttgt tgtcccctgc agttgacaat acgtcgaaga caccgtcacc
    15781 caattcaagg atagagacgt cgaatgtacc accaccaagg tcaaatacca agattttttc
    15841 ttctttgtca gtcttgtcca aaccataagc aagagctgct gcagttggtt cgttaacaat
    15901 acgttctact tcaagaccag caattttacc agcgtctttt gttgcttgac gttgagcgtc
    15961 gttgaagtaa gccggaactg tgataacagc tttggttact ttctcaccaa ggtagtcttc
    16021 agcgtagcct ttcaagtatt gaaggatcat agctgagatt tcttgtggag tgtattcttt
    16081 tccatttgca gaaacttttt cagaagttcc catcttagat ttgatagaga taactgtatc
    16141 tgggtttgta actgcttgac gttttgcagc atcaccaacg atgatttctc cgtttttgaa
    16201 tgagactaca gatggagttg tgcggtttcc ttctgggttt gcgatgattt tgctttcagt
    16261 tccttcaaga actgcaactg ctgagtttgt tgtacctaag tcaataccga taattttaga
    16321 catgtgtttt tctccttgtt agttaatttt ctatttttct ttttgatact cttcttaagt
    16381 ggcgacgccg tcagagcttg acttcgtcaa tctctttgac taaactttga gcctaaggtc
    16441 tcaaaatttg cgcaaatagc accacggcga agagtatcgt ctttggttcg cttcgctcac
    16501 tattacaaag ccaaacatat tttatctttc ttcatagttt attgtcgttt cggacaagtt
    16561 ttctattatg taaattgcga cacgaggagt cgaaatcgat tttatttcga cgacgagtta
    16621 gtaaggaagc taggcaaacg tcatagcgat tgccgttttc tgacgagttg ctttagctac
    16681 cgtcagaatt gccgaatcga acacgcccta agctctgagt aaaaaagata gattttcttg
    16741 tgttcatcga acacttcgtc aatctcctat ttttactgcg agctttttac gagctttgta
    16801 tcttagttat acaccactac cattgctggg cgtaggatgc ggtcatggag tttgtagcct
    16861 ttttgaaaga cttgggcgat ggtatctact gggtgttcat cgtctgctgg gagagtttgg
    16921 atggccatat ggtagttatg gtcaaattcg ccatctgctg cgatttcttc aattccttct
    16981 tctttcaaag cgtgaatcaa gctttcttgc accatcccca agcccttctt cacatcatct
    17041 gtcaaacctt caactgcaag tgcacgctca aggttgtcaa gagatggtaa gattgctttt
    17101 gccaagtcct ggctacgata acgttgcaag ttttgacgtt cttcattggc acggcgttgg
    17161 atattttgca tttctgcatg agcgcgaaga tatttgtttt cgaactcatc tgcacgttca
    17221 tttgccaagt ccaactcaga cttttcagga gttgtttctt cagctgtttc cacaacttcc
    17281 tcttcttgaa cttcttctac ttcttcattt tttatatctt gggccatgtt cgcctccttt
    17341 aatgatttca atcttaatgt acttcgtaat gattactgct gaggtagcgg taaaaatctg
    17401 tcaacttcat ggtcaaaaca cggttgacca cattgacttg attgattagc tgttggtaat
    17461 ccagattaac tggaccgata atggctagaa ttccaactcc ccgataagga atgaggaact
    17521 tactactaat caccgctagg tcagctaaac aggactcttg accgtctgca acacgaacat
    17581 tttgcatctg atcctcacgc aacccctcac gaatctccaa ggccaccttt tgcggttggt
    17641 caaagaactg ataggcttct agattggcaa aattcaagag attgaccttg cccgccatca
    17701 caatgttttc gttgaacatt tccttaaaga tgtgttcaaa gagatcgatg acattatccg
    17761 ttgttgtaaa gtaacgctgg ataatctgcg gaatctccgt ccgaatcttg tagtgaatat
    17821 ctaaaacggt gtgaccgagg aaacgttcct gaatgatgct cttcagtttc agcaaatcct
    17881 cctgcaagaa gttccttgga atcagaaact gactagtaac cgttcgcgac tcgtctaggg
    17941 taaataccgc caaggctgta tgttgcccca aaacaacgat atcaaaggct gtcaaacgtt
    18001 gcctgctcgg ctcaacatcc agtgccacta ccgtacagcc actcaggcct gttagcaagt
    18061 tagcagcctc ttgcagaata tcctccaatt tgaagaattc ctgatcaaag gctttgacaa
    18121 tctcatatac ctcattttca gccagccggt gaaaatccag tgagtgtttc acatagtact
    18181 gaaaaccagc aacacttggc atccgaccgc ttgaagtatg agccttctca agcaaccctt
    18241 gtttttctag ttccgccatg tcattacgaa tggttgcact gctagagtta atagactctt
    18301 gcaaggcttt tgatccgaca ggttcgtgcg ttttggtaaa gatgtcaata atcagattta
    18361 aaatatcctg ctgacgctct gtaaccatct catcactcct ctctgtctga tcaattagca
    18421 ctcaatgcat ccaagtgcta acttatgatt ctattataca cccttaaaag agaatgtcaa
    18481 gacaaaaact caaaaaatta gcactctttt atcaagagtg ctaaaaatca atctgaagac
    18541 ctagaacctt gctattcatc tactcggtat ttccttttta gtctgaaata accatgaatt
    18601 agataagaaa tcagaaaagc atataaaaca aaaataagaa agaaagacat agaaagatcc
    18661 tctctaaaca aactcgtaca aacaacaaga ccacctaaga aagtagttgt acaggaacga
    18721 agtctagatc gcataacttc ccatttgagc cccttactca tatagacttg atcctctgga
    18781 agtaaatcag aagacgattt acgaagaatc gaacaagaac ctgctcctat caattcccaa
    18841 cctctatctc taaaatcttg cagttcatgc ttatattttt taagaaatct agaatcatag
    18901 atacggtaga tgacatcgtc tggttgacat tggtcaaaat agaacaaacc aaaacgactc
    18961 gttctatacc tccaaccttt caaatgcatc tcatgtaaat attcttcttc cttgtccaaa
    19021 tcaacaatgg tgaaaatccg aaattctact ctgctattca ttgtcttacc ccaaaattag
    19081 aaaacatgcc tggcgttatt tattagataa ttctttccac ttttgactca atctccaaaa
    19141 aatataagaa atctgaatcg caaaaactat caataaaacc caatctatta tgaaaatcaa
    19201 aaacactttc caactgaaag aactacctcc agtgacaaac tttgagcaaa acggtagtag
    19261 agctaaaaag agaagtaaaa taggaagcat ccgcattgtt aaaatccgtt tggcataaaa
    19321 aaatctttat ttaaactaaa atattatagc aaaatttacg ccagtttttg aacggctgat
    19381 gtagatattt tatactttca aaatgtttaa atgtgattat ttatttttga aaaatagctc
    19441 accagcccga ctgaaagtgc ttatagaatg ataataagtc gcctgccgaa aacagcggaa
    19501 aatagcggtg ttatgcggag ataatctgac gcgatgcgaa agtatattgc atacttattt
    19561 tcaacaattt agcagagtat ttttataagt gtgatataat agaagtataa tttgttctga
    19621 tagtttattt tatggagaag tagattttta gaatgcggag ggttcaatat ggttgagttt
    19681 ataaagtcta agaaagaaat gagtgaggag gatattaaag caaatttcat cactcctgct
    19741 attgtatcca aaggatggaa aaatggtgag catatcgctt acgaagaata cttcactgat
    19801 ggtcgaattg aggttagagg agataaggct cgtcgtaaag aaggaaaaaa atcagactat
    19861 tcactgtatt accaatttgg aactcgaatt gcaattgttg aggcaaagga taataaacac
    19921 agcgttcgag caggattaca acaagctatt gaatatggag agattttaga tgttccattt
    19981 gtttattctt cgaatggtga tggctttatt gaacacgacc gtatcacgag agaagaacgt
    20041 gagctggagt tagacgaatt ccctactcgt gaagaattat tttctcgtat gacgaaggaa
    20101 aaaggattga cgtacgaaat tacagaagct atctcaactc catactatac agacgccttc
    20161 tcaatgaaaa cgccacgcta ttatcagcaa atagctatca accgtactat tgaaacagtt
    20221 gccagaggac aaaaacgagt aatgtttgtg atggcaacag gaacagggaa aacgttcatg
    20281 gcttttcaaa ttattcatcg ccttcgaaaa gctggtttgg ctaaacgagt tttattctta
    20341 gcagatagaa acatcttagt agaccaaacg atggctgaag actttaagcc attcgaaaag
    20401 gtaatgacga aaattacacc aaaacttttg actgctcctg aaaaattaaa ttctttcgaa
    20461 atttatctag ggctttatca gcaactaact ggtgaagatg gaactgaaac acattatcaa
    20521 aaatttgaca aagacttctt tgatttaatc gtaattgatg aagcgcaccg tggttcagct
    20581 aaggaaaaca gtaactggcg taaggtaatt gattatttca gttctgcgac acagattggg
    20641 atgaccgcta ctcctaaaga aaccaagaat gcttccaata cggaatactt tggtaagcca
    20701 atctatactt atagtttaaa acagggaatc gaggatggtt ttttggctcc atatcgtgtt
    20761 atgagggtta atttagatgt ggatgtggat ggttatcgtc cagaaactgg aaaagttgat
    20821 gctaacggac aattaataga agataggtac tacggcagga aagattttga taaaaccatt
    20881 gtcattgatg atagaacgca aagagttgcc aagtttgttt ctgattatat gaagcaaaac
    20941 aatgcacgat ttgataaaac aattgttttt tgtgttgata ttgaccatgc cgagcgaatg
    21001 cgtgctgcac ttgtaaaaga gaatctagac ttagtccaag aagactatcg ttatgtcatg
    21061 caagtaactg gtgacaacgc tgaaggaaaa gctcaactgg ataactttat ggatgtcaat
    21121 tctaattttc ccgctattgt aacaacgtct aaattattaa cgacaggagt taatgctaaa
    21181 acatgtcgtt tgattgtttt agactctaat atccaatcca tgactgaatt taaacaaatt
    21241 attggtcgtg gcacacgtct ttatcctcaa aaggggaaag aattttttac gattattgat
    21301 tttcgaaatg ttaccaattt gtttgctgac cctgattttg atggtgagcc agtgaaggtg
    21361 ctggaaacag gtgcgaaaac agtcagtggt tctacgcctg gtttcgtagg tgaggaaggt
    21421 gacccagtag aaaaatatat cgttacagac aagcaggtta ccattcttaa ttctactgtt
    21481 caagtattgg atgaaaacgg gaaactgatt accgaaagcc tgaccgacta cactcgaaag
    21541 aatatcttag gtagctacgc cactttgaac gattttatca cagtttggca tacggcagat
    21601 aagaaacatt atgggaagtt tgctgatgga agcactcaag aaattgatgt tccttatgat
    21661 attcctgata cttgggagtg ggtgaggttt tctacattgg ttgaaattgt cagaggtggc
    21721 tctccacgac caatcaaaga ttatcttact tctgaagtag atggaataaa ttggataaaa
    21781 ataggtgata ctgaaaaggg tgaaaagtat ataaataatg ttaaagaaaa aatcaaaaaa
    21841 tcagggctta acaaaactag atttgtaaaa aaaggtacat ttttgttaac taattctatg
    21901 agttttggta gaccttatat tttgaatgtt gatggtgcaa tacacgatgg atggttggct
    21961 atttcgaact atgaaaactc attaaataaa gattacctat tctatattct ttcatcaaat
    22021 gtagtttatt ctcaatttct atctctaatt agtggagctg ttgtgaaaaa cttgaatagt
    22081 gataaagttg cttctattct tatc
//
LOCUS       DAVBCV010000033        22017 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBCV010000033 DAVBCV010000000
VERSION     DAVBCV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.033"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            51..233
                     /locus_tag="VQ516_001261"
     CDS             51..233
                     /locus_tag="VQ516_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2511701.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
     gene            533..1372
                     /locus_tag="VQ516_001262"
     CDS             533..1372
                     /locus_tag="VQ516_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2511702.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            1390..3228
                     /locus_tag="VQ516_001263"
     CDS             1390..3228
                     /locus_tag="VQ516_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2511703.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            3241..4656
                     /locus_tag="VQ516_001264"
     CDS             3241..4656
                     /locus_tag="VQ516_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2511704.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            5248..6294
                     /gene="pheS"
                     /locus_tag="VQ516_001265"
     CDS             5248..6294
                     /gene="pheS"
                     /locus_tag="VQ516_001265"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2511705.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            6294..6803
                     /locus_tag="VQ516_001266"
     CDS             6294..6803
                     /locus_tag="VQ516_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511706.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            6880..9285
                     /locus_tag="VQ516_001267"
     CDS             6880..9285
                     /locus_tag="VQ516_001267"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2511707.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYVLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPATPFVEITKFPAVSRDVALLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(9458..10459)
                     /locus_tag="VQ516_001268"
     CDS             complement(9458..10459)
                     /locus_tag="VQ516_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2511708.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIADDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            10615..11103
                     /locus_tag="VQ516_001269"
                     /pseudo
     CDS             10615..11103
                     /locus_tag="VQ516_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11187..11453
                     /locus_tag="VQ516_001270"
     CDS             11187..11453
                     /locus_tag="VQ516_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2511709.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            11714..13963
                     /gene="metE"
                     /locus_tag="VQ516_001271"
     CDS             11714..13963
                     /gene="metE"
                     /locus_tag="VQ516_001271"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2511710.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            14027..14893
                     /gene="metF"
                     /locus_tag="VQ516_001272"
     CDS             14027..14893
                     /gene="metF"
                     /locus_tag="VQ516_001272"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2511711.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            15578..17791
                     /gene="pnp"
                     /locus_tag="VQ516_001273"
     CDS             15578..17791
                     /gene="pnp"
                     /locus_tag="VQ516_001273"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2511712.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDDQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            17807..18424
                     /gene="cysE"
                     /locus_tag="VQ516_001274"
     CDS             17807..18424
                     /gene="cysE"
                     /locus_tag="VQ516_001274"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2511713.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFIW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            18436..19320
                     /locus_tag="VQ516_001275"
     CDS             18436..19320
                     /locus_tag="VQ516_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511714.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            19402..20745
                     /locus_tag="VQ516_001276"
     CDS             19402..20745
                     /locus_tag="VQ516_001276"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2511715.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYEVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEGLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            20738..21124
                     /locus_tag="VQ516_001277"
     CDS             20738..21124
                     /locus_tag="VQ516_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2511716.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLISLMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            21128..22012
                     /locus_tag="VQ516_001278"
     CDS             21128..22012
                     /locus_tag="VQ516_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2511717.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         6765 a         3965 c         4860 g         6427 t
ORIGIN      
        1 accccaaaag ttagattttt tctgtctaac ttttggggtg tagttcacag ttgaagagcc
       61 agaaaattaa agtgtctttt actcaatcaa aaatagtaac gcctattctt ttggtgaata
      121 agactattgt ttggtatggt gcaatgccat tattagggaa ggtagatgag atgaccatat
      181 tacgtttgga atcagctagt atagtttctg aactagtggc aggtttacga tagagaaaat
      241 ttttaaaaat ttctatgtat gattttcatt tctttagtga gactgttgcc attatcacat
      301 tcgaatcaca caaaataaaa aaatttctac aagtacttga caaatagatt gaaatatcat
      361 aaaatgaaaa tggttacaga gttattaatt atttaagctt catgtcacca ttaaaaattg
      421 aaataaaagg atgttatcac taatacaagt gagcaggaac ctatttaatc acatcggaag
      481 aagtttcttg atgtttttaa gtaggttcct tttattttaa aagggaaatt ttatgatcat
      541 aaaacgaata ctaaaccaca atgccgtaat tgcgcaaagt aaaaaagata tcgatattct
      601 tctttttgga aggggcatag cttttggaag aaaaactgga gataaagtaa atccaattga
      661 tattgagaaa agtttttttc tcaaaaatag agataatatg acccgtttta cagagatgtt
      721 tattaacgtt cctttggagt tggtgtacat caccgaaaaa ataattaacc taggtaaaat
      781 aacattgggt aataattttg atgaaattat ctatattaat ttaacggatc atatttcttc
      841 gagcatagaa cgttataaag aagggattat tatttcgaat cccctacgct gggaaatatc
      901 gaaatattat aaagaagaat ttgaacttgg gaaaagggct ttacaaataa taaaaaaaga
      961 gttaggtatt gaacttccaa ttgacgaagc tgcattcata gcgctacatt ttgttaatgc
     1021 taatttagaa aataattttc aagagtcgta taaaatcact gaaataatta tgggaattga
     1081 gaaaatcatt caagatttct attgtactga gtttaaccaa gattctattg attattatag
     1141 attcataact catataaaat tatttgctca tcgcttggtt gaaaatacaa cttattgtga
     1201 cgatgatgat gaagacttgt tagcattaat gaaaaataaa tatcctagag aatatgaatg
     1261 tggtgaacag gtggcaatgt ttatacaaac tgagtacaat tatttgctga cttctagtga
     1321 attagtttat ttgatggctc atattcgtcg cttaacaaaa aatttagatt aatagaatag
     1381 gagaatacca tgagttataa agatacggta caaaaaatcc tcgatgtaat tggaggtgaa
     1441 aaaaatgtca atagagttac ccattgtgta acacgtttaa gattagaatt aaaagatgaa
     1501 aatttagtca atgatgatga tgtgaagaag ataccagatg taataggtat tatgaaaaag
     1561 aatggacaat atcaaattat acttggtaat gatgtagcta attattataa agaattcgtt
     1621 aaacttggca attttgaatc cgattcagtt gttcaagggc acaaagggaa tattttagaa
     1681 agaatcattg agtatatcgc tggttccatg actccaatca ttccagcaat gttaggggga
     1741 ggtatgttga aagtcttggt aatcatttta ccaatgcttg gtatattgca atcagattct
     1801 cagactattg cttttttgac attttttggg gatgctccat attatttctt accgctgtta
     1861 ttagcttatt ctgcatcaca aaaattaaaa gtaacatcta cattagctat gtctgtagca
     1921 ggtgtacttc tccatccaaa ttttgttcaa atggtgcaat cagggaatcc tcttagttta
     1981 tttggtgcac ctgtgacacc agctagttat ggttcatcag tcgttccaat tcttattatg
     2041 gtttggttga tgaaatatat tgaaaaaata attgctaaat taacaccagc tattactaag
     2101 agttttttgc aacctacgct agtattatta gtatcaagct gtattgcctt agttgtagtc
     2161 ggacctattg gagtaattgt tggtgaagga ttatcaaatc tagttgggca aatgtatggt
     2221 gtagctggat ggcttacatt agctattctt ggtgctatta tgccatttat tgttatgact
     2281 ggaatgcatt gggcttttgc acctattttt ttggcggcat ctattgctac tccagacgta
     2341 ttaattcttc cagcaatgtt agggtcaaac ttagctcaag gggctgcttc gatggctgtt
     2401 gcattaaaga gtaaaaataa taatacaaaa caaattgctt ttgcagcagg tttctcagcc
     2461 ttacttgcag ggattaccga acctgcatta tatggtgtga ctttaaaata taaaaaaccg
     2521 ctttatgcag ctatgattgg tggtggatta gcgggattat ttgcaggtct tactagtgtt
     2581 aaagcatatc tatttgctgt cccatctttg atagcgttgc ctcaatttat ttattctgat
     2641 gtgccatcaa atattgtaaa tgctttaatt gtggcggtca tttcggttgt tattaccttt
     2701 gtattagctt atatatttgg aatcgatgaa gaagagagtt ctagcaattt agaagttaaa
     2761 gctggagttt caaataaaaa aatgatattt tctcctatat caggagaaat cattccgtta
     2821 agcgatgtcc aggataaaac attttcagat aaattaattg gagacggagt agcgattatc
     2881 ccaagtgaag gtaaggttta tgcaccattt gatgggaaaa ttacaaatat ttttccgact
     2941 aagcacgcaa ttggattgaa gagtgatgag ggtgttgagt tactaattca tattggatta
     3001 gatactgttg agctaaaagg tcaaggtttt attagtcatg tagaagaagg agacagagtt
     3061 ttcaaaaatc agttgatttt tgaaatgaac ttgaatttaa tcaagactaa aggctacgaa
     3121 acagttacac cagtaattgt aacgaatacc aatgattttc tagatgtatt agtattacct
     3181 aataatcaga caatcgagca ttctaaggaa ttactggtaa tattataatg gaggaaagtt
     3241 atgaccattt ttccagatga ttttctttgg ggtggagcgg ttgcagctaa tcaagtagag
     3301 ggagcatata atgaagatgg taagggctta tcagttcagg atgtgttacc caaaggtgga
     3361 ttaggagaag caacagaaaa tcctacagaa gataacttaa aattgatagg tattgatttt
     3421 tatcataaat ataaggaaga tatatccttg ttttctgaaa tgggctttaa tgtttttcgt
     3481 acttctattg catggagtag aatttttcca aaaggagatg aggaagaacc taacgaagct
     3541 ggtttgaaat attatgatga attatttgat gaactacatg ctcacgggat agaaccactt
     3601 gtaactcttt cacactatga gactccatta tatttagcaa gaaaatatca tggatggatt
     3661 gataggagaa tgattcattt ttatgaaaaa tttgctcgaa cagttttaga aaggtataaa
     3721 gataaagtta aatattggct tacatttaac gaagtaaact ctgttttgga attaccgttt
     3781 actagtggag gaatagatat acctaaggag aatctttcga aacaagaatt atatcaagct
     3841 atacatcatg aattagtcgc ctcgagtttg gttacaaaaa ttgctcgtga gattaattca
     3901 gagtttaagg tggggtgtat ggtattagct atgccagctt atccaatgac tccaaatcca
     3961 aaagatgtat gggcgactca tgagtatgag aatctaaatt atctattttc agatgtgcat
     4021 gttagaggtt attatccgaa ttatgcaaaa agatatttta aggaaaatga cattaacata
     4081 gagtttgcag ctgaagatgc agagttatta aaaaattata ctgtagattt tttatccttt
     4141 agctattaca tgagtgtgac tcaatctgct attccaacac agtataattc aggagaaggg
     4201 aatattattg gtggtttagt aaatccttat ttagagtctt cagagtgggg atggcaaatt
     4261 gatccaattg gactacgtat aattttaaat agatattatg accgttatca aatcccatta
     4321 tttatagtag aaaatggatt aggtgctaaa gatcaactaa taaaagatga acttaataac
     4381 ttaaccgtcc aagatgatta tagaattcag tatatgaaag aacatttatt gcaagttgct
     4441 gaagctctac aggatggtgt tgaaattatg ggctatacgt cttggggatg tattgactgt
     4501 gtttctatgt ccactgcaca actttctaaa aggtacggtc tcatttatgt tgatcgaaat
     4561 gatgatggaa atggcacatt caatcgatat aagaaaatgt cctttacttg gtataaagga
     4621 gtgattgaat caaatggaga atccttattc aaatagtttg tttattgatg attagatatt
     4681 atcacggtta aggcaacatc tcattaaata taaaataaag aggtttggaa gacatcaaat
     4741 tctaaacctc tttattttat agggggatta attaatttaa ttttgttaca gagagccgat
     4801 tataaatagt taacttagga tagattggtt atatatagta gactgaatct aaaatagtac
     4861 gaaacaattg ctaaaacatt tatagaaatt aattttactt tcccaatcga tttgttttca
     4921 tcttatttca atccgctata aagttagata agaataagct aacaagcaga agtaagatag
     4981 gtcttgcaag aatggtaaaa atctgataaa ctaagtagta attgaatagg aatctgaaag
     5041 actagtagcc caagggaagt atatcaggga ggagagccgt gactgcaagc tctctatatg
     5101 aaagctgggt gaattcactt gcgcatggat ttagaaatga aggtctgact tgtcagatga
     5161 aaacggatgg taccgcgtgt caacgctccg agtggagttt ttggcatgtg gttttctttt
     5221 tatctacgag agactgatgg aggaaatatg tcaactattg aagaacaatt aaaagcgctt
     5281 cgcgaagaaa cgctggctag cttgaagcag attactgctg gaaatgaaaa agagatgcaa
     5341 gatttgcgtg tctctgtcct tggtaaaaag ggttcgctca ctgaaatcct caaagggatg
     5401 aaagatgttt ctgctgagat gcgtccaatc atcgggaaac acgtcaatga agctcgtgat
     5461 gtcttgactg ctgcttttga agaaacagct aagctcttgg aagaaaagaa agtcgcggct
     5521 caactggcta gcgagagtat cgatgtgacg cttccaggtc gtccagttgc gactggtcac
     5581 cgtcacgttt tgacacaaac cagtgaagaa atcgaagata tcttcatcgg tatgggttat
     5641 caagtcgtgg atggttttga agtggagcaa gactactata actttgaacg tatgaacctt
     5701 ccaaaagacc acccagctcg tgatatgcag gatactttct atatcactga agaaatcttg
     5761 ctccgtaccc acacgtctcc agttcaggcg cgtgctatgg atgcccatga tttttctaaa
     5821 ggtcctttga agatgatctc gccagggcgt gtcttccgtc gcgatacgga cgatgcgacc
     5881 cacagtcacc aattccacca aatcgaaggc ttggtagttg ggaaaaatat ctctatggct
     5941 gatcttcaag gaacgcttca gttgattgtc caaaaaatgt ttggtgaaga gcgtcaaatt
     6001 cgtttgcgtc catcttactt cccattcaca gagccatctg ttgaggtgga tgtttcttgc
     6061 ttcaagtgtg gtggagaagg ctgtaatgta tgtaagaaaa caggttggat cgaaattatg
     6121 ggggccggta tggttcaccc acgtgtcctt gaaatgagtg gtatcgatgc gactgtatac
     6181 tctggctttg cctttggtct tggacaagag cgtgtagcta tgctccgtta tggaatcaac
     6241 gatatccgtg gattctacca aggagatgtc cgcttctcag aacagtttaa ataatgatta
     6301 gaaaagtaga aatggcagat gttgaggtgt tggctaaaat tgccaaacaa gcctttcgtg
     6361 aaacctttgc gtatgataat acggaagagc agttacagga atactttgaa gaggcttata
     6421 gtctgaaaac tttgtcaact gagttgggaa atcctgactc tgaaacctat ttcattatgc
     6481 atgaggagga gatagctggt tttctcaaag tcaactgggg aagtgctcaa actgagagag
     6541 aattagagga cgcttttgaa attcaacgcc tctatgtgct acaaaaattc caaggatttg
     6601 gactaggtaa gcaactgttt gaattcgcac ttgaacttgc tacaaaaaat agtttttctt
     6661 gggcttggct aggtgtttgg gagcataata caaaagctca agcgttttat aaccgatatg
     6721 gttttgaaaa atttagccaa catcatttta tggttggtca aaaagtagat acggattggt
     6781 tactgagaaa gaaattaagg taagaagaga attcttctat tgaaatgata ggaaaggaaa
     6841 agaaccagtg tggcaatggt cattctggag aactaaatta tgcttgtatc ttataaatgg
     6901 ttaaaagaat tggtgggcat tgatgtgcca tcacaggagt tggctgaaaa aatgtcaacc
     6961 acagggatcg aggtcgaagg tgtcgaatca ccagctgccg gtctttcaaa aattgtcgtc
     7021 ggtgagatct tgtcttgcga agatgtgcca gaaactcacc ttcatgtttg tcaggttaac
     7081 gttggcgaag aagaagagcg tcagatcgtt tgtggtgccc caaatgtgcg tgcaggcatc
     7141 aaggttatgg tggctcttcc gggagctcgt atcgctgaca attacaaaat caaaaaagga
     7201 aaaatccgtg gcttagagtc acttggaatg atctgttcac ttggtgaatt gggtatttct
     7261 gactcagttg tacctaagaa attcgcagat ggtattcaaa ttttgccaga aaatgccgtt
     7321 ccaggtgagg aagtcttttc atatctagac ttggatgatg aaatcatcga actttctatc
     7381 acaccaaacc gtgcagatgc cctttctatg cgtggagtgg ctcacgaagt ggcagccata
     7441 tatgacaagg cagtcaactt taaagaattt actttgacag aaacaaatga agctgcgaca
     7501 gatgcccttt ctgtgggcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg
     7561 gacaatgtga ctatcgcacc aagtccacaa tggttgcaaa accttctcat gaacgaaggc
     7621 atccgtccca tcaataacgt tgtagacgtg acaaactaca tcctgctcta ctttggtcaa
     7681 cctatgcatg cttttgactt ggacacattt gaagggactg acatccgtgt gcgtgaagcg
     7741 cgtgctggtg aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac
     7801 ctagtcatca ctgttgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca
     7861 acagaaatct ctgaaaaatc tagtcatgtt gtccttgaag ctgctgtttt caatggcaaa
     7921 tctatccgta agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa
     7981 aaaggaatta atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg
     8041 gaacttgcag gtgcgacggt gcgtaagggt atcgtttcag cgggtgagct tgatacttca
     8101 gatgtagaag tttcttcaac tcttgctgat gttaaccgtg tcctcggaac tgagctgtct
     8161 tatgctgatg tcgaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac
     8221 agctttacag tcagcgtacc acgtcgtcgt tgggatatca caatcgaagc tgatctcttt
     8281 gaagaaatcg ctcgtatcta tggatatgac cgcttgccaa ccagccttcc aaaagacgat
     8341 ggtacagctg gtgaattgac tgtgacacaa aaactccgcc gtcaagttcg taccattgct
     8401 gaaggagcag gtttgacaga aatcatcacc tatgttctaa caactcctga aaaagcagtt
     8461 gagtttacgg ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt
     8521 tcagtcctcc gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct
     8581 cgtaagaata aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat
     8641 ccaaaagaag aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt
     8701 gctgaaaaag atttccaaac agcagcagtt ccagttgatt tcttctatgc taaaggaatc
     8761 ctagaagcgc tctttactcg cttgggacta gaagtgacct atacagcaac agctgagatt
     8821 gccagccttc acccaggacg tacagcagtc atttcactcg gtgaccaagt tcttggtttt
     8881 cttggccaag tgcatccagt cactgctaag gcttacgata ttccagaaac gtatgtggct
     8941 gagctcaacc tttcagtcat cgaagctgcc cttcaacctg ctactccatt tgtggaaatc
     9001 acgaaattcc cagcagtcag ccgtgacgtt gcccttctcc tcaaggcagc agtaactcac
     9061 caagaagtta tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc
     9121 tttgacgttt tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg
     9181 accttccaaa atccagaaga cagcttaacg gacgaagaag tcgcacgcta catggaaaaa
     9241 atccaagcat cgcttgaaaa aaaagtgaac gcagaagtgc gttaaacgat agtaggatac
     9301 aaattccccc tctagtttat gaacactaga ggggtgattt tatataatac tcaatgaaaa
     9361 tcaaaaagca aactaggaag ctagccgtaa gctgtacttg agtactgtaa ggcgaggctg
     9421 acgtggtttg aagagattgt cgaagagtat aaaataatta aaatgtgatt tctgtaaaaa
     9481 tacatctatg atcagagttt tgaaaactta caatgtctaa atctttaaca tagtagtggt
     9541 ttttaggcaa taaaatatga tctattgttg cattaaaaag ttttggactt tggctattcc
     9601 aagttcctct ttcaaaaggt ggtagtacat ttaatgcgtc cctatgagag cttatttttg
     9661 caagtgctcc atgacgcata gttgcattaa aatcatctgc aataatagcc tttggatatt
     9721 ttgaagccaa ttgattatga atgatgttta agtcttgctt ccagatttcc attaaacctg
     9781 gcagaggagg agcagtatgc aaggcaatga tatctggtat attttgtttt ctcgaatgta
     9841 atacaattgt cccgaaccgt gttgtatgaa aagttttagc ttctgtatag aaaccataac
     9901 ttttcttgac aatcacagtc acaggagcta ttccactatt ggtaggtgga gaagtgaaaa
     9961 tatcatagtt ggccatagaa agtccaactt gatgaaacaa tagtttgatt ctctggtttt
    10021 cttgctcacc tctgatattg gtagctagtt cagggaatat agccatatcg gcgtcaaaat
    10081 ggctaaaaat tcgctcaata tgttgtgctt ctatttgatt agcgacgttc caagttacta
    10141 attttaaggt tttagtctta tttgatgcag aagaaagggt tttatctgtt ccgaataata
    10201 gtaatatgag cgatagtaag agcaaaatgc cagagtaaaa ccgtatttgt ttatttttct
    10261 tgtaaaaaga tagggaaaaa aatacgaaac ttaaaatagc agccatagtt gcagtagcta
    10321 aggggaaggc ataaatctgt gccaagatag gtctggtaga tagccaaata ttaagattag
    10381 aagatggaaa aaaagaataa gttatgatag ggataacaaa gagaatgacc gaaagggcag
    10441 agagaaatgt tttaatcatt gtgtgagtac ctcaatttgc aatggttgct cggagctaat
    10501 gtgactttgt gactttactt gattgatatt ctttttcttg tcttcctcat agattaagta
    10561 caacccaagt aaaaaaagtc caatttaaga ttgaaatgat taaaaggcac agaaatgcaa
    10621 gacaattata ctacaaaagg caaacatttg acaatcgata gccgtcgctt aatcgaaaga
    10681 tggaaaaaag aagggaaatc aaatagagaa attgcctctc tgcttggaaa agttcctcaa
    10741 actatccaca ctgaaatcaa gcgtgggaca gtccgacaat gtcttggaaa agggcgcttc
    10801 aaagaggttt attctgccga ctatgctcaa cagtcttatg aaaacaatcg caagcgctcg
    10861 gtcaagaaat caagcttgac caaggaacta aaggaaaaga ttctccacta tcataaccaa
    10921 aaattttcgc ttgaaatgat ggtgatggct aaaggggtta acgtgggaat ttcaaccatt
    10981 tactattgga ttcatcgtgg aaaattgggg ttaagcaagc aggatctgct ttaccctaga
    11041 aaaggaaaag cgcttaagaa acaggctagc accaacttta aacctgctgg tcaatctatc
    11101 gaatcataat tcgctcattc ttttaaaaat acatagacgt aaaagaaaga gggttaagtg
    11161 atgaagaata gtgctattgg gagtaattgg aaggatgtcc atctgagctc tttagcaagg
    11221 aggaaatcct tgaaagtgat atgcgagtgg ctatcatgag cgagttgatt gaggctaggt
    11281 atgaacaagg aatcagtcag aaaaagctgg aagaagtcag tggagtgagt caacctgtca
    11341 tagctaggat ggaaacagga gagagcagtc ttcagttgga tacggtctta aaagttctaa
    11401 ccagtctagg aaagacacta gcagtcgttc cacttgaaca ggggaaaagt tgactagaat
    11461 cagttatagt cttttctaat aacaggtcat agtcacttat aagaattact aataacatgt
    11521 tttagtattc taattggaat atagctgtaa agtaagaatt acaaaggatt tgagacgttt
    11581 gcttcaagtc tttttctttt gtccgaaaca gatatatagt ttcaaactat atcaaaccct
    11641 aattttttac aattatacag acgctttctt ttctgttaag atagtttcaa caacaaattt
    11701 tggaggacac atcatgtcaa ctacaatcat cggtttccct cgtttgggcg aattccgtga
    11761 attaaaattt acaactgaaa aatactttag aaaagaaatc tcagaagaag aacttttagc
    11821 agcagcaaaa gacttgcgtg ctaaacactg gaacattgtc aaagaaaaag gcatcactga
    11881 aattccatca aatgactttt ctcactatga taacttccta gatgcggctt tccttttcaa
    11941 cgtggtgcct gcatccgttc aaaacttgga cttgtctgac cttgagcgct acttcgcttt
    12001 gggacgtggt taccagggag aaaaagggga cgttcgcgcc cttccaatga agaaatggtt
    12061 caacactaac taccactaca tcgttcctaa atttgaaaaa gacactcaag taaaattggc
    12121 tggtcacaag attttcgatg agttccaaga agccaaagaa cttggattga acactcgtcc
    12181 tgttcttgta ggtcccttca ctttccttca attgtcagac tttgaagaag gcgtgaaagc
    12241 agacgacttc gtagatagct tagtggctgc ttaccaagaa gtttttgcta aattggctga
    12301 acttggtgcg actcgcatcc aattggatga agcggctctt gtaaaagact tgacagctga
    12361 agaaaaagct ctcttcttga acctttacaa caaactcttg gctgacaaaa aaggtcttga
    12421 agtcttgctt caaacttatt tcggtgatgt tcgtgacgta tacgctgacc ttgtaaactt
    12481 accagtagat gctatcggtc ttgactttgt tgaaggtaag aaaactcttg aactcgttaa
    12541 aggtggcttc ccagctgaca agactctcta tgtaggtatt gtcaatggta aaaacatctg
    12601 gcgcaacaac tacgaaaaga gcttggctgt tcttgagcaa attccagctg aaaacatcgt
    12661 tttgacaagc tcttgctcac ttcttcatgt gccatttaca actgctaatg aagaatttga
    12721 accagcactc ttgaaccact ttgcctttgc agttgaaaaa ttggatgaaa tccgtgactt
    12781 ggatgctatc cgcaatggtc aaggttcaga agctcttgca gccaacaaag aactctttgc
    12841 gactgagcgt gttggtgaaa atgctgaact tcgtgcgcgt atcgctggct tgacagacgc
    12901 agactacact cgtttgccag cctttgcaga acgtgaagct atccaagaag aagctttcaa
    12961 acttccagct cttccaacaa caacgattgg ttcattccct caaacaaaag aagttcgtgc
    13021 caaacgtttg gcttaccgta aaggtgaatt gtctcaaaaa gagtacgatg ctttccttgc
    13081 tgaaacgatt gacgaatgga tcaagtggca agaagatatt gactttgatg tccttgttca
    13141 cggtgaattt gagcgtaatg acatggttga gtacttcggt caaaacttgt caggatacct
    13201 cttctctaaa aatggatggg tacaatcata cggtatgcgt ggggtaaaac caccaatcat
    13261 ctggggtgat gtcactcgtc ttaaccctat cactgttaaa tggtctagct atgcacaaag
    13321 tcgtacaaac aaacctgtta aaggtatgtt gactggacct gttaccatcc ttaactggtc
    13381 attcccacgt gaagacatct ctatcaagga ttctactctt caaatcgccc ttgctatcaa
    13441 ggatgaagtg cttgaccttg aagctgctgg tgtgaaaatc atccaaattg atgaggctgc
    13501 tcttcgtgaa aaattgccac tccgtcgtag tgactggtac gaagattacc ttgactgggc
    13561 aattcctgcc ttccgcttgg tacactcaac agtagcgtca gacacacaaa tccacactca
    13621 catgtgttac tcagaattta cagatatcat cccagctatc gataacatgg atgcagacgt
    13681 tatttccttt gaagccagcc gttcaaacct tgaaatcttg gacgaactca aagcgaaaaa
    13741 cttccaaaca gaagtgggac ctggggttta cgatatccac tcacctcgtg taccaaatga
    13801 aggcgaaatc gacaacacaa tcgaagccat ccttgctaaa gtgccaagca agaaagtttg
    13861 gatcaaccct gactgtggtt tgaaaacacg tggtatccca gaaacaaaag aaagcttgat
    13921 ccgccttgtt gaagcagcta aagctgcgcg tgagaaattg taagattgga tttctctcca
    13981 tacagtggtt gttcaaaaag aaatcaacta gaaaaggtta ttacatatgt cacgccaaac
    14041 accgtcactc tcatttgaag tgttccctcc aaacccagcc gtgggtaatg gtaacattat
    14101 ttcagccttg caggatatgc aggagttggc tccccatttt atcagtgtaa ctgccagcaa
    14161 taataaattt aatatcaagg aaacgacggt tcgtttggct gactttatcc aaaatgactt
    14221 ggcgattccg accattgccc acttgccagc tatttatcta actaaggaca aggttgctga
    14281 aactattgct gacttggaca aagttggggt tcagaaaatc ttggctcttc gtggggatat
    14341 tattccagac gtagaaccac aaaaggattt ccgctatgca accgacttga ttgagtttat
    14401 caaggaacaa gcccctcact ttgatattat tggagcttgc tatccagaag ggcatccaga
    14461 ttcgccaaat cagatttcag atattcaaaa tcttaagaag aaagtggatg caggctgttc
    14521 gagcctcgta actcagctct tctttgacaa tgagcgcttc tatgattttc aggacaagtg
    14581 tatcttggct gggattgatg ttcctattca tgcaggaatt atgccaattc tgaatcgaaa
    14641 tcaggctctc cgactcttga agacttgtga gaatatccat cttccacgca aatttaaagc
    14701 catcttagac aagtatgagc atgaccctga gtcgctcaga gcagcaggac ttgcctatgc
    14761 agtggaccaa atcgtggact tggtaactca ggatgttgcc ggtgtgcatc tctatacgat
    14821 gaataatgct gatacagcaa aatacatcca tcaagcaacc catgccttgt ttaatcacca
    14881 gtctctagga taataaaagc aaaccattct tctcaggtga ggggaatggt tcctttttaa
    14941 tggtaaagac ctcacttttt aagaaaaata tgataaaata gattctgtac gtacttgata
    15001 caaagatgaa ggtattaaat aatacaatgc atttaaactg taagatttaa cgacacgagt
    15061 gagtcaaatc gatgttattt gacgaaagag ttagtaaagc atttagctaa tcgcccgata
    15121 gcgattagcg tgaaaggttt agatacttta atattaaacc tttctaatga tgtatcttga
    15181 aaactgaaca cgcctacaac tctgtggaaa aagataaatc tgcctaggag cagtcgctcc
    15241 gtcgttcgat ttcctatctt cctttgagtt gcttgacggc ttggtatctt gatgacttga
    15301 attttggtct catttcttag gaaaaaagat aagcttccta gcactcgtcg agtgcgacgt
    15361 caccttccta tttttctaca gaaatgttcg gcaagccgaa ccgtccaaaa tatcttgtgt
    15421 aattgaacac ggccgaaaag ctgtgtaaaa aagataaact gtcttgtctt catcgaagac
    15481 ttcgtcagtt tcctattttt actttgcttt tgacgtcctt ggtatcttga tctttgtagg
    15541 caaggcgtat aatttcatca atccaaaggg gattaaaatg gcaaaacaag tgtttcaaac
    15601 gacttttgcg ggtcgtgagt taattgtaga gactggtcag gttgctaagc aagcaaatgg
    15661 ctctgttgtc gtacgttacg gtgagtcaac tgtcttgact gctgccgtta tgtctaagaa
    15721 aatggcaact ggggattttt tcccactcca agtcaactac gaagaaaaaa tgtatgcggc
    15781 tgggaagttt cctggtggct ttatgaaacg tgaaggacgt ccttcaacag atgcgacctt
    15841 gacagcgcgt ttgattgacc gtccgattcg tcctatgttt gcggaaggtt tccgtaatga
    15901 agtccaagtc atcaatacag tgctttctta tgatgaaaat gcatctgcac caatggctgc
    15961 tatgtttggt tcatctttgg cactgtctat ttcagatatt ccatttgacg gaccaattgc
    16021 tggggtacaa gtgggatatg tagatgacca aatcatcatc aacccaagtc aagaacaagc
    16081 agagcaatct cttcttgaat tgacagtagc tggaactaag cacgctatca acatggtaga
    16141 gtctggtgcc aaagaattgt cagaagaaat catgttggaa gcgcttctta aagggcacga
    16201 agctgtcaaa gaattgattg ccttccaaga agaaatcgtt gctgctgtcg gtaaagaaaa
    16261 agcagaagtg gaattgcttc acgtggatgc tgaattgcaa gctgaaatca ttgcagccta
    16321 caacagtgac ctccaaaagg cagttcaagt agaagagaaa ttggcccgtg aagctgcaac
    16381 tcaagcagtg aaagaccaag tgactgccgt ttacgaagaa aaatatgcgg accacgaaga
    16441 atttgaccgt attatgcgtg atgtggctga aatcttggaa caaatggaac acgcagaagt
    16501 gcgacgttta attacagaag acaaggtgcg tcctgatggt cgtaaggtcg atgaaatccg
    16561 tcctttggat gcggttgttg acttccttcc tcgtgtacat ggttcaggtc tctttactcg
    16621 tgggcaaact caagctcttt cagtcttgac cttggctccg atgggagaaa ctcaaatcat
    16681 tgatggtttg gatccagagt acaagaaacg ctttatgcac cactataact tccctcaata
    16741 ttctgtaggg gaaacaggtc gttacggtgc gccaggtcgt cgtgaaatcg gtcacggtgc
    16801 ccttggtgag cgtgctcttg ctcaagtctt gccaagcttg gaagaattcc catacgctat
    16861 ccgtctagta gcagaagttt tggaatcaaa cggttcttca tctcaagctt ctatctgtgc
    16921 gggaactctt gcccttatgg ctggtggtgt gccaatcaag gcgccagtag ctggtattgc
    16981 tatgggactt atctcagatg gaaataacta cacagtattg acagatatcc aaggtttgga
    17041 agatcacttt ggagatatgg acttcaaggt tgcaggtact cgtgatggga ttacagccct
    17101 tcaaatggat atcaagattc aagggattac tgcagaaatc ttgacggagg ctcttgctca
    17161 agccaagaaa gcgcgttttg aaatccttga tgtcattgaa gcaaccattc cagaagttcg
    17221 tctagaattg gctccaactg ctccgaaaat tgatacgatc aagattgatg tggacaagat
    17281 taagattgtc atcggtaagg gtggagaaac catcgacaag attatcgctg aaacaggtgt
    17341 taagattgat atagacgaag aaggaaatgt gtctatctac tctagtgacc aagatgctat
    17401 taaccgtgcc aaagaaatta ttgctggttt ggttcgtgaa gccaaagtgg atgaagttta
    17461 ccgtgctaaa gtcgttcgta tcgagaaatt tggtgccttt gttaacctct ttgataagac
    17521 agatgccctt gttcatatct ctgagatggc ttggactcgt accaatcgtg tagaggattt
    17581 ggtagaaatc ggggatgaag ttgatgttaa ggttatcaag attgatgaaa aaggccgtat
    17641 cgatgcctct atgaaggctc ttctacctcg tccgccaaaa cctgagcatg atgaaaaagg
    17701 tgaaaagtct gagcgtcctc accgcccacg tcatcacaag gatcacaaac ctaagaaaga
    17761 atttacagaa acaccaaaag attcagaata agaaaaggag aaatgtatgg ggtggtggcg
    17821 cgaaaccatt gatatcgtaa aagaaaatga tccagcggcc cgcaccactt tggaggtttt
    17881 gctgacttat ccaggtgtca aggccttggc ggcccaccgt ctctcgcatt ttatctggaa
    17941 gcacggcttc aaactcttag ctcgtatgca cagtcagttt tggcgctttt ggactcagat
    18001 tgagattcat ccaggagccc agattgattc aggtgtcttt attgaccatg gttctggtct
    18061 ggtgattgga gagacagcga ttgttgaaaa aggcgttctt ctctatcacg gagtgactct
    18121 tgggggaaca gggaaagact gtggcaaacg ccatccgact gtacgaaagg gagccctcat
    18181 atcagcccat gcccaagtta tcggacctgt ggaaattggt gaaaatgcca aggtcggtgc
    18241 tgcagcagtt gtcgtagcag atgtacctag tgatgtgacg gttgtcggta ttccggccaa
    18301 gattgtccgt cttcatggta agaaagatga gcctgtcatt cacgaagtcg aagaaaaacg
    18361 agagtattat gtcaataaac tcgagcaggc taaagatgcc agtcacagat cgtctggttt
    18421 gtagaggata cctatatgat tcaagtaaga aacaagttaa gccaagagga gctatctgag
    18481 gcgaaaaaag taattaactg ttgccaaaac tatgacggta cctatcgcga tccctatctc
    18541 tctaacatgc ttaattttga cccaaacatg cccgcctttt tcctttatta tgaaaaaggc
    18601 gaacttgttg gtttattaac tgtctatgca gatgaccaag atgtagaagt gacgatactg
    18661 gttcatccag gtcatcgccg tcaggggatt gcgcgtgcat tgtttactag ttttgagaga
    18721 gaaacagctt ctttccccat tcgatcagtc acttttcaga cagaacgtat ttttttagag
    18781 aatcatcctg actttgccag taactgggga ctaatcgagg atgaagagac agaaacttgg
    18841 ttaggtaagg atagaagacc ttatccgtta gcaaatgttt cccatattga agttttgtta
    18901 gcagatagtt cgtatcagga tcaaattagt caattaaaat ttcaggcatt ttcagaggaa
    18961 catgaatcga gagaagttgt ggatagatat gtcgctgaag ctctgaagga tccagaaagc
    19021 cgactatata ttttgttaaa agacggtcag gttattggga cttgcacggt agatttatcg
    19081 actaatacga attacttcta cggtttagca atatcggaac ctgaacgtgg gaaaggctat
    19141 ggaagctact tagcaaaatc cctcgtcaac caactaattg agcaaaatga caaggaattt
    19201 cagattgcag tggaagatag caatgtaggt gccaaacgtt tgtatgaaaa aattggcttt
    19261 gtcaaacaga ctcaggtggt ttatctgaat gagaaaggag caagggattc cgaagtgtag
    19321 agatattcgg actgaaattt acttgaactt ttagtgatga aactaattgt tcttggattt
    19381 cagctttctt gattatgatt tatgattaaa atctatgaca ccatgtctcg tgatttgcga
    19441 gaatttgtcc cgattgagga cggcaagatc aagatgtatg tttgtgggcc aacggtgtac
    19501 aactatattc atgtagggaa tgctcgttca acggtagctt ttgataccat tcgtcgctat
    19561 tttgagtatc gtgggtacga ggttgcctat atttccaatt ttacggatgt ggatgataag
    19621 attatcaacc gtgccaggga agaaggcatc acgcctcagg aggttgcgga taagtacatc
    19681 gctgcctttc gtgaggatgt gacggccttg ggcgtgaaac ctgcgactcg ccatccgcgt
    19741 gtagtggagt ttatggcaga catcatccgt tttgtggaag gcttgatcga gaaaggcttt
    19801 gcctatgaga gtcaagggga tgtctatttc cgtgtagaaa aatcccacaa ctatgctaaa
    19861 ttggctaata aaaccttgga agatttggag ctaggtgctt caggtcgtac cgatgaagaa
    19921 acggctcgta aggaaaatcc tgtagacttt gctctatgga aatcttccaa accaggtgag
    19981 atttcttggg atagtccttg gggacctgga cgtccgggtt ggcatattga gtgttcagtc
    20041 atgtcgacag agattttagg tgataccatt gatattcacg gtggtggagc tgacctagag
    20101 tttccacacc acaccaatga aattgcccaa tctgaagcta aaacaggcaa ggcttttgct
    20161 aactactgga tgcacaatgg ctttgtcaat atcgacaatg tcaaaatgtc taagtccttg
    20221 ggtaacttca ttacggtaca cgatgccctt aaaactcttg atgggcaagt acttcgtttc
    20281 ttctttgcga cccagcacta ccgtaagcct atcaacttca cggaaaaggc agttcgcgat
    20341 gccgagacca atctcaagta tctgaagaat acttacgaac aaccatttac aggaaacgtg
    20401 gatgcccaag agttacaaaa ctttaaagat aagtttgtag cagctatgga tgaagatttt
    20461 aatgctgcca acggtatcac agttgtcttt gaaatggcca aatggatcaa ctcagggaac
    20521 tatgatgcaa gtgtcaagca agctcttgca gatatgttag aaatttttgg aattgtcttt
    20581 gttgaggaag ttttggatgc agagattgaa gacttgattc aaaaacgcca agaggcgcgt
    20641 gccaatcgtg actttgcgac agcagaccaa atccgtgacc aattggttac tcaaggaatt
    20701 aagctccttg ataccaagga tggagtgagg tggacacgtg attgatgtca atctcattaa
    20761 cgggattgcg ctagcctttg agggagatgc ggtgtattct atgtatattc gccgccacct
    20821 catcctcaaa ggtatgacca agcccaataa actccaccaa gaggcgacca agtacgtgtc
    20881 agccaaggct caagctcgcc tgatttctct catgttggag gagcagatcc taacggaaaa
    20941 agaagaagaa atctacaaac gtggccgcaa taccaatagt cacacaaagg ctaaaaatgc
    21001 agatgtcgtg acttatcgta tgtccacggg atttgaagca gttatgggct atctccatat
    21061 gactgagaat ctggaacgtc ttgagagttt ggtttcatgg tgcatccaaa aagtggaggg
    21121 ctagaacatg agggcaaaag aactacaaga ctggtttcct gaggctcgga tttcagacca
    21181 accagtagag aaagagggct atctcacgct tcctttagct tctcagcagt ggattttgct
    21241 ggaggaagct gggctcagcg agcgtgaaaa gcagttggtt gcccttttga cccagcagga
    21301 gcaggctcgt tcgctaaacc cttggtattc ctatctggtt gaaggcaagg gacaggcacc
    21361 gcaagttttt aaaaagattc agttggttta ttgccatctt tcttattttc agcaggaaaa
    21421 tctggcttct tggctagata tgatgcggac tctttttccg aattgtcaga cagtgttaca
    21481 ggtcggagct caggattatg ttttcgtgct tcaacaagac aaatacactt ctgtaagaga
    21541 tattttaagt gatatgattg aagcagttga gtatgacttt ggacttcgtc tttctatcat
    21601 gttgggtcag gtttggtctc agacaggaca tcaagcccta tcagacttaa tcaaagctga
    21661 gcgggatttg ttcaagacat ggtggcgtca gggtcaccaa ggtgttcata ctttttctca
    21721 gctctatctt tggagtatgg gagaaagact cgtggacttg aagccaatta aagaatgtct
    21781 acaccagatg attttggatc aagatcagat tcaggaaatc attctctctc tttgggaaaa
    21841 tagtgctgtt ctcactaaaa cagcccagca actctatctg caccgcaatt ctctccaata
    21901 caagattgat aaatgggaag agttgacagg gcttcagttg aaagagttga ccgacctgac
    21961 cttgtgttat caattgattt taggttcttt gtcaactata gttggtttgt aaagaag
//
LOCUS       DAVBCV010000034        21460 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBCV010000034 DAVBCV010000000
VERSION     DAVBCV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.034"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            66..287
                     /locus_tag="VQ516_001279"
     CDS             66..287
                     /locus_tag="VQ516_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2511718.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            804..1043
                     /locus_tag="VQ516_001280"
                     /pseudo
     CDS             804..1043
                     /locus_tag="VQ516_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1187..2416
                     /locus_tag="VQ516_001281"
     CDS             1187..2416
                     /locus_tag="VQ516_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2511719.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2436..5132
                     /gene="ppc"
                     /locus_tag="VQ516_001282"
     CDS             2436..5132
                     /gene="ppc"
                     /locus_tag="VQ516_001282"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2511720.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5215..5432
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5545..6549
                     /locus_tag="VQ516_001283"
     CDS             5545..6549
                     /locus_tag="VQ516_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2511721.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6618..7484
                     /locus_tag="VQ516_001284"
     CDS             6618..7484
                     /locus_tag="VQ516_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2511722.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7481..8239
                     /locus_tag="VQ516_001285"
     CDS             7481..8239
                     /locus_tag="VQ516_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511723.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8329..10089
                     /gene="dnaG"
                     /locus_tag="VQ516_001286"
     CDS             8329..10089
                     /gene="dnaG"
                     /locus_tag="VQ516_001286"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2511724.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10092..11201
                     /gene="rpoD"
                     /locus_tag="VQ516_001287"
     CDS             10092..11201
                     /gene="rpoD"
                     /locus_tag="VQ516_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2511725.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11215..11544
                     /locus_tag="VQ516_001288"
     CDS             11215..11544
                     /locus_tag="VQ516_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2511726.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11659..12702
                     /gene="cpoA"
                     /locus_tag="VQ516_001289"
     CDS             11659..12702
                     /gene="cpoA"
                     /locus_tag="VQ516_001289"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2511727.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12718..14043
                     /locus_tag="VQ516_001290"
     CDS             12718..14043
                     /locus_tag="VQ516_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2511728.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14074..14250
                     /locus_tag="VQ516_001291"
     CDS             14074..14250
                     /locus_tag="VQ516_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2511729.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14309..14443
                     /locus_tag="VQ516_001292"
     CDS             14309..14443
                     /locus_tag="VQ516_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2511730.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14507..15817
                     /gene="obgE"
                     /locus_tag="VQ516_001293"
     CDS             14507..15817
                     /gene="obgE"
                     /locus_tag="VQ516_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2511731.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15827..15985
                     /locus_tag="VQ516_001294"
     CDS             15827..15985
                     /locus_tag="VQ516_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511732.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17035..18294
                     /locus_tag="VQ516_001295"
     CDS             17035..18294
                     /locus_tag="VQ516_001295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2511733.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18310..18861
                     /locus_tag="VQ516_001296"
     CDS             18310..18861
                     /locus_tag="VQ516_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511734.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            18854..20131
                     /locus_tag="VQ516_001297"
     CDS             18854..20131
                     /locus_tag="VQ516_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2511735.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20147..21007
                     /locus_tag="VQ516_001298"
     CDS             20147..21007
                     /locus_tag="VQ516_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2511736.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            21230..21340
                     /locus_tag="VQ516_001299"
                     /pseudo
     CDS             21230..21340
                     /locus_tag="VQ516_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
BASE COUNT         6560 a         3687 c         4816 g         6397 t
ORIGIN      
        1 ccagttagga cactttgaat gatttcttag ttttatatat tatacttttc tcaggagaaa
       61 gctagatgta cagacgtttg agagatttga gggaagatca cgatttgacc caaaaacaaa
      121 tagctaaaat actttcgttt acaaactcag cttatgctaa aattgaacgg ggtgagcata
      181 cgttaacagc agatgtattg gtaactctct caaactttta cgatgtcagt acagactacc
      241 tattggaatt gacagatttt cctgataaaa ttcgctttag aaaataatct cctcaatttc
      301 atagagtgtg aaaatgagga gattttttat ttgttctttg aaaattaaat agtccaaaat
      361 ggtactttcc tcatttgtgg agcaaatttg aatggctcgc catgataaga gcgattttaa
      421 agccatcaat aaaatagagc gatactttat atgccatgat acaaatgata tacaatgata
      481 cttctgaccg ttcaggctgc catcgtaaaa gagcagcaag tgaaattctt atgatgactt
      541 catcagtcat gccacgttga atgtgtgagt ttgttagaga aacgcaatta atcctcaaaa
      601 ggttccccga accttttgag ttccacagac gcatcacgtg gagtgtgtaa gcttgttgct
      661 aaaagcgtaa aaaccttgga acgaaaggaa taatagactt cctgcgaaac aaaaatacaa
      721 tacaataaaa ctatggatga tgaagcaagt aaacaattaa gcgatagccg tttcaagagc
      781 cttgtaggtg ttcagcgcac gagatgttag ctgtgttaaa aacagcttat caacgcaacg
      841 taaacgcgca aaaggtggac gaaaaaccaa attaagccta gacgatctcc tcatggtaac
      901 tattcaatac atgcgagaat agcgcactta tgaacaaatt gcggctgatt ttggcattca
      961 cgaaagcaac ataattagtc ggaggtcaat ggattgaagc aactcttatt caaaatggtt
     1021 ttacgatttc aaattctgcc ttaattctgt aaaaacagta aaattcgaag gattgtaagg
     1081 taagagtttt tttctttctg aaaaaatggt ataatagcaa tcaaaactag aaaataaaac
     1141 ggaatttgga acagatttgt ctgtatccta gtagagtggt gatactatga agattagtaa
     1201 gaggcactta ttaaattatt ccatcttgat tccctacttg cttttatcta ttttgggctt
     1261 gattgtggtc tattcgacca ccagtgctat tttaattgaa gaaggcaaga gcgccttgca
     1321 gttggttcga aaccaaggaa tcttttggat tgttagtttg atactgattg ccttaattta
     1381 taaattgaga ctagattttt tgagaaatga gcgactaatc attttagtta tattaataga
     1441 aatgctttta ttgttcttgg ctcgttttat tggtatttca gtaaacgggg catacggttg
     1501 gatttcggtt gcaggagtaa ctattcagcc agctgagtac ttaaaaatca ttattatttg
     1561 gtatttagct caccgattct ccaaacagca agaagaaata gctacttatg attttcaagt
     1621 tttgactcaa aatcaatggc ttccccgtgc ttttaatgat tggcgattcg ttctcctagt
     1681 tctgattgga agtttgggaa ttttccctga tttaggaaat gcgactattt tagtcttggt
     1741 ttccttgatt atgtatacag ttagtggaat cgcttatcgc tggttttcaa ccattctggc
     1801 gctcgtatct gccgcttctg tctttgtctt gaccactatc agcctaatcg gtgttgagac
     1861 cttttcaaaa attccagtat ttggctatgt agccaaacgc tttagtgcct tttttaatcc
     1921 ttttgccgat cgtgctgatg caggtcacca gttagctaat tcttattttg ccatggtcaa
     1981 tggtggttgg tttggtctag gtcttggaaa ctcgattgaa aaacgaggtt atttgccaga
     2041 agctcataca gactttgtct tttctatcgt gattgaagaa tttggctttg ttggtgccag
     2101 tcttatttta gctctcttgt ttttcatgat tttgcggatt atcttggtcg gtattcgagc
     2161 ggagaatcct ttcaatgcca tggttgcact cggtgtcgga gggatgatgt tggttcaggt
     2221 atttgtcaat atcggaggga tttcgggctt gattccatct acaggagtaa ccttcccctt
     2281 cttatcccag ggtggaaata gtcttctagt cttatcagtg gcagtagcct ttgtcttaaa
     2341 tattgatgcc agtgaaaaac gcgctaagtt gtaccgagaa ttggaaaatc aaccaatgaa
     2401 ccttctgttg aagtaggata aagaaaggat agtttatgtc tcttcaaaaa ttagaaaatt
     2461 ctagtaataa aagtgttgtg caagaagaag tcttgattct aacagaatta ctggaagata
     2521 ttactaaaaa tatgcttgcc ccagagacct ttgaaaaaat aatacagttg aaagaattat
     2581 caacgcagga agattatcaa ggtctaaacc gtctagtgac tagcttatca aatgatgaaa
     2641 tggtctatat ttcacgctat ttctctatct tgcctctttt gattaatatt tcagaggatg
     2701 tggatttagc ttatgaaatc aatcatcaaa ataatattga tcaggactat ttaggtaaat
     2761 tatctacaac gattaaattg gtagcagaaa aggaaaatgc cgttgagatc ctagaacact
     2821 tgaatgttgt tccggttttg acagcccatc caacacaagt gcaacgcaaa agtatgttgg
     2881 atttaacaaa tcatattcat agtcttttgc gtaaataccg tgatgttaag ttggggttga
     2941 tcaataaaga taaatggtac aatgatttgc gtcgttacat cgaaattatc atgcagacag
     3001 acatgattcg tgagaaaaaa ttaaaagtga ctaacgaaat cacgaatgct atggaatatt
     3061 ataacagctc ctttttgaaa gctgtacctc atttgacgac ggagtataag cgcttagcgc
     3121 aagcgcatgg tctgaattta aaacaggcta aaccaatcac catgggtatg tggataggtg
     3181 gtgaccgtga tggaaatcca tttgttacag caaagacctt gaagcagtct gcactcactc
     3241 aatgtgaagt catcatgaac tactatgata aaaagattta ccaactttat cgtgaatttt
     3301 ctctttcaac tagcattgtc aacgtcagca agcaagtcag agaaatggct cgtcaatcca
     3361 aggataactc gatttaccgc gaaaaagagc tttaccgtcg tgccttgttt gatattcaat
     3421 caaaaattca ggcaactaaa acctatctga ttgaggatga agaagttggg actcgttatg
     3481 aaaccgccaa tgatttctac aaggatttga ttgccattcg agattctcta ctagagaata
     3541 agggcgagtc cttgatttca ggtgattttg tggaattatt gcaggcagta gagatatttg
     3601 gtttttacct agcatcaatt gatatgcgac aagactctag cgtctatgaa gcctgtgtgg
     3661 cagaactctt gaaatcagca ggaattcatt ctcgttatag cgagttgagc gaagaagaaa
     3721 agtgtgacct tcttttgaaa gaattagaag aagatccccg aattctttct gcgactcacg
     3781 cagaaaaatc agaattatta gcaaaagaac tagctatttt taagacggct cgtgttttga
     3841 aagataagtt gggagatgat gtcatccgtc agaccatcat ttcacatgca accaaccttt
     3901 ctgatatgct agaattagct attctgttaa aagaagtagg actggtggat acggaaaggg
     3961 cgcgtgttca gattgttccc ctttttgaaa caattgaaga cttggatcat tcagaggaaa
     4021 caatgagaaa atatctttct cttagccttg ccaaaaaatg gattgactca cgaaataact
     4081 accaagaaat catgcttggc tactctgaca gtaataaaga tggcggttac ttgtcatcat
     4141 gttggaccct ctacaaggct caacaacaat tgactgctat tggagatgaa tttggcgtta
     4201 aggttacctt cttccatggt cgtggtggta ctgtcggtcg tggtggtggg ccaacctatg
     4261 aagccattac atctcaaccg ctcaagtcta tcaaggatcg tatccgtttg acggagcagg
     4321 gtgaagtaat tgggaataaa tacggtaaca aagacgccgc ttactataac cttgaaatgc
     4381 tagtatcggc agctattaac cgtatgatta ctcagaagaa gagcgatacc aataccccaa
     4441 atcgttatga agccattatg gatcaagtag tggaccgtag ttacgatatc taccgtgatt
     4501 tggtctttgg taatgagcat ttctatgatt atttcttcga gtcaagtcca atcaaggcta
     4561 tttcaagttt taatattggt tctcgtccag ccgctcgtaa gactattact gaaatcggtg
     4621 gtttgcgtgc catcccttgg gtattctcat ggtcacagag tcgtgttatg ttccctggat
     4681 ggtacggggt tggttcaagc ttcaaggaat ttatcaataa aaatccagag aatattgcta
     4741 tcttacgaga tatgtaccaa aattggcctt tcttccaatc gcttctttca aatgttgata
     4801 tggttttgtc aaaatcaaat atgaatattg cttttgaata tgctaaactt tgtgaagacg
     4861 agcaagttaa ggccatctat gagattattt taaatgaatg gcaagttact aagaacgtta
     4921 tcttggctat tgaaggacat gacgaactct tagctgacaa tccatatcta aaagctagtc
     4981 tggattaccg tatgccttac tttaatattc tcaactatat tcagttagag ttgattaaac
     5041 gccaacgtcg tggagaattg tccagtgatc aagaacgatt gattcatatc accatcaacg
     5101 gaattgcgac aggattgcgt aattcaggtt gataattttc aagagtgaat gctaaaagtg
     5161 aatatcaaaa aaattctaat agactattga caagtagttt aaaaatgata taatttaacc
     5221 attcagaaaa gtaatcatac aaacttttta gagagtctgt ggtagctgaa aacagataag
     5281 tggcgatgat gaaaattggg ctgaatgcta tttagaattt gaaattataa aaattcggta
     5341 agcacacctt acagtgcatc tcgttattgc gagactgagc gatagggaaa ttccctataa
     5401 ttgaggtggt accgcgcatc gacgtcctca cacaagtttt ttgtgtgagg atttttttga
     5461 tggaggttag tatggaaaga aaacgatggc gtcgcttgtt tagataagtg aaatatgtta
     5521 aaggaaataa aaaggagaaa cagaatgaaa aataaacgtt taattggaat tattgctgca
     5581 ttagcagtct tagtagcagg aagcttgatt tattcttcaa tgaataaatc agaagctcag
     5641 aataataagg atgagaagaa aataaccaag attggtgtgc ttcaatttgt gagccatcca
     5701 tcccttgatt tgatttataa agggatccaa gatggacttg cagaagaagg atataaagat
     5761 gatcaagtta aaattgattt tatgaactca gaaggtgatc aaagtaaggt tgcgacaatg
     5821 agtaaacaat tggttgcaaa tgggaatgac cttgtggttg gtattgcaac accagcagcc
     5881 caagggttgg ctagtgcaac aaaagaccta ccagttatca tggccgctat tacagaccca
     5941 attggtgcta acttggttaa agatttgaaa aaaccaggtg gtaacgttac aggggtatct
     6001 gaccacaatc cagctcaaca acaaattgaa ctcatcaagg ctctgacacc gaatgtgaaa
     6061 acaatcggag ctctttactc aagtagcgaa gacaattcaa aaacacaggt tgaagaattt
     6121 aaggcttatg ctgaaaaagc aggtctgaca gtggaaacat ttgcagttcc ttcaacaaat
     6181 gaaattgcct caactgtcac tgttatgact atcaaggtag atgctatttg ggttccaatt
     6241 gataacacca ttgcatcagg atttccaacg gttgtctcta gcaatcaaag ttctaagaaa
     6301 ccaatttatc ccagtgcgac agctatggta gaagtaggtg gtttggcatc agttgtaatt
     6361 gaccaacatg accttggtgt ggcaacaggt aaaatgattg tgcaagtctt gaaaggtgca
     6421 aaaccagccg ataccccagt caatgtcttt tcaactggta agtcagtcat caataaaaaa
     6481 atagcacaag aactaggtat tactattcct gagtctgttc tcaaagaagc aggacaagtc
     6541 atcgaataaa taaggtaggg aggaggagac tcttcccatt ttttaaaaag atgaatatag
     6601 aaaggattaa ataaaatatg attgtttcca ttatttctca aggatttgtc tgggctattc
     6661 taggtctggg aatctttatg acatttagga ttttaaactt tccagatatg acgacagaag
     6721 gttccttccc tcttggggga gctgttgctg tcactttgat aaccaaaggc gtgaacccat
     6781 ttttagcgac acttgttgct gtaggagcag gttgtttggc tggaatggca gcaggccttc
     6841 tttatacaaa agggaagatc ccaaccttgc tctcagggat tttggtgatg acttcttgtc
     6901 actcaatcat gctcttgatt atgggacgtg cgaatttagg cctgcttgga accaagcaaa
     6961 ttcaggatgt tttgcctttt gattcggatt tgaatcaact cttgacaggt ctcatctttg
     7021 tgagcattgt tattgctctc atgctctttt tcttggacac taaactcgga caagcctata
     7081 ttgctacagg ggataatcct gatatggcta gaagtttcgg gattcatact ggacgcatgg
     7141 agctcatggg cttggtctta tcaaatggtg tgattgccct tgcaggtgcc ctcattgctc
     7201 agcaagaagg ctatgccgat gtgtctcgag ggatcggggt tatcgttgtg gggcttgcaa
     7261 gtttgattat tggagaagtt attttcaaga gtttgagctt ggcagagcgt ttggttacta
     7321 tcgttgtagg ttctatcgct tatcaatttt tagtgtgggc agttatcgca cttggcttta
     7381 atacaagtta ccttcgttta tacagtgcct tgattttagc agtctgcctc atgattccaa
     7441 catttaagca aacaatcttg aaaggagcca agttaagcaa atgacagcaa ttgtagaatt
     7501 aaaaaatgca accaaaattg ttaaaaatgg ctttgatgaa gaaaagatta ttttaaatga
     7561 tgtttcctta gaaatttttg aacgggactt tatcacgatt ttgggcggaa atggtgctgg
     7621 aaaatcaacc ctctttaaca ctatagcagg gaccttatca ctaactagtg gaactatccg
     7681 tattttaggt gaagatctca ctaagttttc acccgagaag cgtgccaagt acctgtctcg
     7741 tgtcttccaa gatccaaaga tggggacagc tccccgtatg acggtcgctg aaaatctttt
     7801 aatcgccaag tttcgtggtg agaagcgtgg attgttacca cgacgcttga ctagctataa
     7861 ggatgaattt caggcaacca ttgaaaaagt aggaaatggt cttgagaaac acttgaatac
     7921 accgattgag ttcttatcag gtggacaaag acaggctttg agtctcttga tggcaacctt
     7981 gaagcgacct gaattactcc tgttagatga gcatactgct gccctggatc caaagactag
     8041 tgttgctttg atggaattga cagatgaatt tgttaagaaa gatcagctaa cagcccttat
     8101 gattactcat catatggaag atgctctcaa atacggcaat cgcttgattg tcatgaaaga
     8161 aggacggatt atccaagatt taaaccaaga agaaaaagca aaaatgaaaa tctctgatta
     8221 ttatcaactc tttgaataaa gtagggaaaa tgagtgaaat ggtttacttt ttttctgaaa
     8281 taaagtatac tatataaagt aaactatgat aacatggagg tattgtgtat ggttgacaaa
     8341 caagtcattg aagaaatcaa aaacaatgcc aacattgtgg aagtcatagg agatgtgatt
     8401 tctttacaaa aggcaggacg gaactatcta gggctctgtc cttttcatgg tgaaaaaaca
     8461 ccttctttca acgttgtaga ggacaagcag ttttaccact gttttggttg tggtcgctca
     8521 ggtgatgtct ttaaattcat cgaggagtac caaggggtta cctttatgga ggctgtccaa
     8581 atcttaggtc agcgtgtcgg gattgaggtt gaaaaaccgc tttatagtga acagaagcca
     8641 gcctcgcctc accaagctct ttatgatatg cacgaagatg cggctaaatt ttaccatgct
     8701 attctcatga caacgactat gggcgaagag gccagaaatt acctttatca gcggggtttg
     8761 acagatgaag tgcttaaaca tttttggatt ggtttagcac ctccagaacg aaactatctc
     8821 tatcaacgtt tgtctgatca gtatcgtgaa gaggatttac tggattcagg cctgttttat
     8881 ctctcggatg ccaatcaatt tgtagacacc tttcacaatc gcattatgtt tcccctgaca
     8941 aatgaccaag gaaaggtcat tgccttctca ggtcgtatct ggcaaaaaac ggattcacaa
     9001 acttctaagt ataaaaacag ccgatcgact gcaattttta acaaaagtta cgaattatat
     9061 catatggata gggcaaaaaa atcttctgga aaaactagtg agatttacct gatggaagga
     9121 ttcatggatg ttattgcagc ctatcgggct ggaatcgaaa atgctgtggc gtcgatggga
     9181 acggccttga gtcgagagca tgttgagcat ctgaaaaggt taaccaagaa attggttctt
     9241 gtttacgatg gagataaggc tgggcaagcc gcgatattga aagcattgga tgaaattggt
     9301 gatatgcctg tgcaaatcgt tagcatgcct gataacttgg atcctgatga gtatctacaa
     9361 aaaaatggtc cagaagactt ggcctatcta ttaacaaaaa ctcgtattag tccgattgag
     9421 ttctacattc atcagtacaa acctgaaaac agtgaaaatc tgcaggctca gattgagttt
     9481 attgaaaaaa tagctccctt gattgttcaa gaaaagtcca tcgctgctca aaacagctat
     9541 attcatattt tagctgacag tctggcgtcc tttgattata cccagattga gcagattgtt
     9601 aatgagagtc gtcaggtgca aaggcagaat cgcatggaaa gaatttccag accgacgcca
     9661 atcaccatgc ctgtcaccaa gcagttatcg gctattatga gggcagaagc ccatctactc
     9721 tatcggatga tggaatcccc tcttgttttg aacgattacc gtttgcgaga agactttgca
     9781 tttgctacac ctgaatttca ggtcttatat gacttgcttg gccagtatgg aaatcttcct
     9841 ccagaagttt tagcagagca gacagaggaa gttgaaagag cttggtacca agttttagct
     9901 caggatttgc ctgctgagat atcgccgcag gaacttagtg aagtagagat gactcgaaac
     9961 aaggctctct tgaatcagga caatatgaga atcaaaaaga aggtgcagga agctagccat
    10021 gtaggagata cagatacagc cctagaagaa ttggaacgtt taatttccca aaagagaaga
    10081 atggagtaat aatggcaaca aaacaaaaag aagtaacaac atttgacgta caggtagcag
    10141 aatttatccg taatcataag caaaaaggga cagcaacaga tgatgaaatc aatgcaagtc
    10201 tggttattcc ttttaccttg gacgctgatg ggattgaaga tctcttgcaa cggattcagg
    10261 atgcagggat ttctatcaca gataacgaag gaaatccaag tgcgcgtgtt cttagcaatg
    10321 aagaagaacc agaactcagc gatgaggact tgattggatc aacttctgct aaggtcaatg
    10381 accctgtccg tatgtacttg aaagaaatag gggtcgttcc tctcttgacc aatgaagagg
    10441 agaaagagtt ggcactggct gttgaagctg gcgatatcga agccaaacaa cgtcttgcgg
    10501 aagccaatct tcgtttggtt gtttccattg ccaaacgcta tgtcggtcgt ggcatgcagt
    10561 tccttgactt gattcaagaa ggaaatatgg gcttgatgaa ggcggttgac aagtttgact
    10621 attctaaagg gttcaagttt tcaacttatg caacttggtg gattcgtcag gctatcactc
    10681 gtgctattgc ggaccaagct cgtaccatcc gtatcccagt tcacatggtt gaaactatca
    10741 ataaattggt tcgtgaacag cggaatctcc ttcaagaatt ggggcaagat ccgacaccag
    10801 aacagattgc tgaacgaatg gatatgacac ctgataaggt tcgtgaaatc ttgaagattg
    10861 cccaagaacc agtatctctt gaaactccta tcggtgaaga ggacgatagc caccttggag
    10921 actttatcga agatgaagtg attgaaaatc cagtggatta tacgactcgt atcgtcttgc
    10981 gtgagcaatt ggatgaaatc ttagatactc ttacagaccg tgaagaaaat gttctgcgtc
    11041 tacgttttgg actagatgat ggaaaaatgc gcacacttga agatgtgggg aaagtcttta
    11101 acgtaactcg tgagcgtatc cgtcagattg aagcaaaggc tttgagaaaa ctacgccaac
    11161 caagtcgcag caaaccgctt cgtgatttta ttgaagacta agagtgagga aaatatggct
    11221 tatacagaag agcaaattga aaacatcaaa acacggattt taacagcctt ggaagaagtc
    11281 atcgaccctg agttgggaat cgatattgtc aatcttggtt tgatctatga gattcgtttt
    11341 gacggtgaca cagggcaaac agagattgac atgactttga caactatggg ttgtccctta
    11401 gcagaccttt tgacagacca gatttatgat gctatgatag aggttccaga agtaacggat
    11461 actgaggtta aattagtctg gtatccagcc tggacagttg aaaaaatgag tcgctatgcc
    11521 cgcattgccc taggcattaa gtaaacatat aaaagcatgt gagagactag aggaaaagcg
    11581 aggaaatttc ccctcttttc ctctagtctc tcctttcttt tgctgatttt attcaaagaa
    11641 aatgatataa tagtagttat ggagaaaaag aaattacgca tcaatatgtt gagttcaagt
    11701 gagaaagtag caggacaggg agtttcaggt gcttaccgtg aattagttcg tcttcttcac
    11761 cgtgctgcca aggaccaatt gattgttaca gaaaatcttc caatcgaggc agatgtgact
    11821 cactttcata cgattgattt tccctattat ttatcaacct tccaaaagaa acgctcaggg
    11881 agaaagattg gctatgtgca tttcttgcca gctacacttg aaggaagttt gaaaattcca
    11941 tttttcttaa agggaattgt gaaacgctat gtattttctt tttacaaccg gatggagcac
    12001 ttggttgtgg tcaatcctat gtttattgag gatttggtag cagctggtat tccacgtgaa
    12061 aaagtgacct atattcctaa ctttgtcaac aaggaaaaat ggcatcctct accacaagaa
    12121 gaggtagtca gactgcgcac agatcttggt cttagtgaca atcagtttat cgtagtaggt
    12181 gctgggcaag ttcagaaacg taaagggatt gatgacttta tccgtctggc tgaggaattg
    12241 cctcagatta cctttatctg ggctggtggc ttctcttttg gtggtatgac agatggttat
    12301 gaacactata agaaaattat ggaaaatccc cctaaaaatt tgatttttcc aggcattgta
    12361 tcgccagagc ggatgcgcga attgtatgct ctagcggatc ttttcttgtt gcctagttac
    12421 aatgagctct ttcctatgac tattttagaa gctgcgagtt gtgaggctcc tattatgttg
    12481 cgtgatttag atctctataa ggtgattttg gagggaaatt atcgggcgac agcgggtaga
    12541 gaagagatga aagaggctat tttggaatat caagcaaatc ctgctgtctt aaaagatctc
    12601 aaagaaaagg ctaagaatat ttccagagag tattctgaag agcatctgtt acaaatctgg
    12661 ttggactttt atgagaaaca agccgcttta gggagaaagt aaaaagtgag gtaatctatg
    12721 cgaattggtt tatttacaga tacctatttt cctcaggttt ctggtgttgc gaccagtatt
    12781 cgaaccttga aaacagaact tgaaaagcag ggacatgctg tttttatctt tacgacgaca
    12841 gataaggatg tcaatcgcta cgaagattgg caaattatcc gcattccaag tgttcctttc
    12901 tttgctttta aggatcgtcg ctttgcctac cgaggtttta gcaaggcact tgaaattgct
    12961 aaacagtatc agctagatat tatccatact cagacagaat tttctcttgg cctgttgggg
    13021 atttggattg cgcgtgaatt gaaaattcca gtcatccata cctatcacac ccagtatgaa
    13081 gactatgtcc attatattgc taaggggata ttgatccggc cgagtatggt caagtatctg
    13141 gttagaggtt tcctgcatga tgtggatggg gttatttgcc ctagtgagat tgtccgtgac
    13201 ttgctatctg attataaggt caaggttgaa aaacgggtca ttcctactgg gattgaatta
    13261 gccaagtttg agcgtccgga aatcaagcag gaaaatttga aagaactgcg tagtaaacta
    13321 gggattcaag atggtgaaaa gacgttgctt agtctttcga gaatctccta tgaaaaaaat
    13381 attcaagcag ttttagcagc ctttgctgat gttctgaaag aggaagacaa ggttaaactg
    13441 gtagtagctg gggatggccc ttatctgaat gacctcaaag agcaagccca gaacctagag
    13501 attcaagact cagtcatctt tacagggatg attgctccta gtgagacggc tctttactat
    13561 aaagcggcgg atttcttcat ttcggcatcg acaagcgaaa cgcaaggttt gacctacttg
    13621 gaaagcttag ccagtggaac acctgtcatt gctcacggaa atccttattt gaacaacctc
    13681 atcagtgata aaatgtttgg agccttgtac tatggagaac atgatttggc tggtgctatt
    13741 ttggaagccc tgattgcaac accagacatg aatgagcata ccttatcaga gaaattgtat
    13801 gagatttcag ctgagaactt tgggaaacga gtgcatgagt tttatctgga tgccattatt
    13861 tcaaataact tccagaaaga tttggctaaa gatgatacgg tcagtcagcg tatctttagg
    13921 acagttttgt atcttccgca acaggtggtt gctgtacctg taaaaggatc tagacgcatg
    13981 ttgaaggctt caaaaacaca gttgatcagt atgagagact attggaaaga ccatgaagaa
    14041 tagaaagagg aacagctatg aaaaaaacaa ttaatgagaa gcggtcgtga taaaaagatt
    14101 gcgggtgttt gtgctggggt ggcccattat ctggatatgg atccgactat cgttcaagtc
    14161 atttggggtg ttcttacttg ctgttacgga gctggaattg tagcttacat tattttatgg
    14221 attatcgcac cagtagcaac tgactattga aaactagctc aattcatgct aagataatag
    14281 aaaatagaat gtaacgaagg agagaaaaat ggcatttgga gataatggaa atcgtaaaaa
    14341 aactatgttt gagaaaataa ccttgtttat cgtgattatc atgctagtag caagtttatt
    14401 gggaattttt gcaactgcaa ttggtgccct cagtaatcta taaaatagat tcaagaaaat
    14461 ttagtgactg ggatttccca gccctttttt aaagtgagaa gaaataatga gtatgttttt
    14521 agatacagct aagattaagg tcaaggctgg taatggtggc gatggtatgg ttgcctttcg
    14581 tcgtgaaaaa tatgtcccta atggaggccc ttggggtggt gatggtggtc gtggaggcaa
    14641 tgtggtcttc gttgtagacg aaggactacg taccttgatg gatttccgct acaatcgtca
    14701 tttcaaggct gattctggtg aaaaagggat gaccaaaggg atgcatggtc gtggtgctga
    14761 ggaccttaga gttcgagtac cacaaggtac gactgttcgt gatgcggaga ctggcaaggt
    14821 tttaacagat ttgattgaac atgggcaaga atttatcgtt gcccacggtg gtcgtggtgg
    14881 acgtggaaat attcgtttcg cgacaccaaa aaatcctgca ccggaaatct ctgaaaatgg
    14941 agaaccaggt caggaacgtg agttacaatt ggaactaaaa atcttggcag atgtcggttt
    15001 agtaggattc ccatctgtag ggaagtcaac acttttaagt gttattacct cagctaagcc
    15061 taaaattggt gcctaccact ttaccactat tgtaccaaat ttaggtatgg ttcgcaccca
    15121 atcaggtgaa tcctttgcag tagccgactt gccaggtttg attgaagggg ctagtcaagg
    15181 tgttggtttg ggaactcagt ttctccgtca catcgagcgt acacgtgtta tccttcacat
    15241 cattgatatg tcagctagcg aaggccgtga tccatatgag gattacctag ctatcaataa
    15301 agagctggag tcttacaatc ttcgcctcat ggagcgtcca cagattattg tagctaataa
    15361 gatggacatg cctgagagtc aggaaaatct tgaagaattt aagaaaaaat tgtctgaaaa
    15421 ttatgatgaa tttgaagagt taccagctat cttcccaatt tctggattga ccaagcaagg
    15481 tctggcaaca cttttagatg ctacagctga attgttagac aagacaccag aatttttgct
    15541 ctacgacgag tccgatatgg aagaagaagc ttactatgga tttgacgaag aagaaaaagc
    15601 ctttgaaatt agtcgtgatg acgatgcgac atgggtactt tctggtgaaa aactcatgaa
    15661 actctttaat atgaccaact ttgatcgtga tgaatctgtc atgaaatttg cccgtcagct
    15721 tcgtggtatg ggggttgatg aagcccttcg tgcgcgtgga gctaaagatg gggatttggt
    15781 ccgcattggt aaatttgagt ttgaatttgt agactaggag actggtatgg gagataaacc
    15841 gatatctttc cgagatgcgg atggtaattt tgtttccgcc gcagacgttt ggaatgaaaa
    15901 gaaattggaa gaactattta atcgtctcaa tccaaatcgt gccttgagat tggcacgaac
    15961 taaaaaggaa aatccatctc agtaaagaag ctaaaaaatc ccgtgcctca tcagacacgg
    16021 gattttgtgg tacgacaggc atgtatagca aactgaatct ggaatagcac agcatatctt
    16081 ctaaaatatt gttataaatt gatttgaatt tcaaaattaa attgtttgat tcttatctca
    16141 atttgttata gtatatctga tgtcaaagtt ctcggcgagt caaatagcga ttcccaagcc
    16201 tgactatcgt gaggtagcgg attaaaatgg tctggggata gaccgtttta agtctgacgc
    16261 tggaaataag aattgtcaga agaagggata gcgaaatcgt ggctctacga acaggaacgt
    16321 gataataagg cgtatatagc ggataagagg gcctcaaact ctaaagtcca aaaaggtagt
    16381 cgtaacctat atgcgtaaat cacgagagta attgaattcg tactaagatt ttctattttc
    16441 actgtaacct tttaacgtcc ttatatcttg tatacacgag gaaagatgta cgacttatcc
    16501 cgtgaggtct atcactataa agagaaaacg acagatagaa gtgatcctga gtcacggtta
    16561 tctttaaaat agattgtctg ataggacggt atgtataaaa cgcttctgtg aactgagaga
    16621 agggggagaa gttcttgcta aaatttagtt gaacagccgt attccgatac ttagataaga
    16681 gatctagtct tagctcctac tcagttttag gggataaaaa aggggaaata gcgattcgag
    16741 aaagattata ctcttcgaaa atctcttcaa atcacgtcaa tatcgccttg ccgtatgtgt
    16801 aggatactga ctacgtcagt tccatctaca acctcaaaac agtgttttga gcaacctgcg
    16861 gctagtttcc tagtttgatc tttgattttc attgagtatt agtaattcag ttactaactc
    16921 gtcaactctg atttatccaa taaaattgaa aaggatggaa aaaaggataa atttatgata
    16981 tactttattt tgaagacctt attagaaatc ttgaaagagt attgaaaact tagaatgaga
    17041 aaaattgtta tcaatggtgg attaccactg caaggtgaaa tcactattag tggtgctaaa
    17101 aatagtgtcg ttgccttaat tccagctatt atcttggctg atgatgtggt gactttggat
    17161 tgcgttccag atatttcgga tgtagccagt cttgtcgaaa tcatggaatt gatgggagct
    17221 actgttaagc gttatgacga tgtattggag attgacccaa gaggtgttca aaatattcca
    17281 atgccttatg gtaaaattaa cagtcttcgt gcatcttact atttttatgg gagcctctta
    17341 ggccgttttg gtgaagcgac agttggtcta ccgggaggat gtgatcttgg tcctcgtccg
    17401 attgacttac accttaaggc gtttgaagct atgggtgcca ctgctagcta cgagggagat
    17461 aacatgaagt tatctgctaa agatacagga cttcatggtg caagtattta catggatacg
    17521 gttagtgtgg gagcaacgat taatacgatg attgctgcgg ttaaagcaaa tggtcgtact
    17581 attattgaaa atgcagcccg tgaacctgag attattgatg tagctactct cttgaataat
    17641 atgggtgccc atatccgtgg ggcaggaact aatatcatca ttattgatgg tgttgaaaga
    17701 ttacatggga cacgtcatca ggtgattcca gaccgtattg aagctggaac atatatatct
    17761 ttagctgctg cagttggtaa aggaattcgt ataaataatg ttctttacga acacctggaa
    17821 gggtttattg ctaagttgga agaaatggga gtgagaatga ctgtatctga ggacagcatt
    17881 tttgtcgagg aacagtctaa tttgaaagca atcaatatta agacagctcc ttacccaggc
    17941 tttgcaactg atttgcaaca accgcttacc cctcttttac taagagcgaa tggtcgtggt
    18001 acaattgtcg atacgattta cgaaaaacgt gtaaatcatg tttttgaact agcaaagatg
    18061 gatgcggata tttcgacaac aaatggtcat attttgtaca cgggtggacg tgatttacgt
    18121 ggggccagtg ttaaagcgac cgacttaaga gctggggctg cactagtcat tgctgggctt
    18181 atggctgaag gtaaaactga aattaccaat atcgagttta tcttacgtgg ttattctgat
    18241 attatcgaaa aattacgtaa tttaggagcg gatattagac ttgttgagga ttaaaccgta
    18301 gaggtgttta tgaatatttg gaccaaatta gcaatgtttt ctttttttga aacggatcgc
    18361 ttgtatttgc gtcctttctt ttttagtgat agtcaggact tccgcgagat agcttcaaat
    18421 ccagaaaatc ttcaatttat tttcccaacg caggcaagtc tggaagaaag tcaatatgca
    18481 ctggccaatt actttatgaa gtcccctttg ggagtgtggg caatttgtga ccagaaaaat
    18541 caacaaatga ttggttctat taaatttgag aagttagatg aaatcaaaaa agaagctgag
    18601 cttggctatt ttttgagaaa agatgcttgg tcgcaaggat ttatgacaga ggttgttaga
    18661 aaaatttgtc agctttcttt tgaggaattt ggcttaaaac aattatttat cattacccac
    18721 cttgaaaatg aagctagcca aagagttgct cttaagtctg gatttagttt gttccgtcag
    18781 tttaagggaa gtgatcgtta cacaagaaaa atgcgggatt atcttgaatt tcggtatgta
    18841 aaaggagagt tcaatgagta agcatcagga aattctaagc tatttggagg aattaccagt
    18901 aggtaaaagg gtcagtgttc gtagcatttc gaatcatcta ggagttagtg atggaacagc
    18961 ctatcgagct attaaagaag ctgaaaaccg tggaattgtg gagacccgtc ctagaagtgg
    19021 aacaattcgt gttaaatccc agaaagttgc tatagataga ttaacgtttg ctgaaattgc
    19081 agaagtgact tcttctgagg ttctggctgg gcaagaaggt ttagagagag aatttagtaa
    19141 gttttcaatt ggtgccatga ctgaacaaaa tatcttgtct taccttcatg atggggggct
    19201 cttgattgtc ggagaccgaa cccgtattca gttgctagcc ttggaaaatg aaaatgcagt
    19261 tctggttaca gggggatttc aggttcatga tgatgtgctt aaactggcca atcaaaaagg
    19321 gattcctgtt ctaagaagta agcatgatac ctttaccgtc gctaccatga tcaataaagc
    19381 cttgtcaaat gtccaaatca agactgatat tctgacagtt gagaaacttt atcgccctag
    19441 tcatgagtat ggttttctga gagagacaga tacagttaaa gattatttgg acttggttcg
    19501 taagaatcgt agcagccgtt tccctgttat caatcaacat caggtcgttg ttggcgttgt
    19561 aaccatgaga gacgctggtg ataaatcacc aagcacgaca attgataagg ttatgtctcg
    19621 tagtctattt ttggttggat tatcgacaaa tattgccaat gtgagtcaac ggatgatcgc
    19681 agaagacttt gaaatggtac cagttgttcg aagcaatcaa actttgcttg gcgttgtgac
    19741 gcgacgagat gtcatggaga agatgagccg ttcccaagtt tcggctctac caactttttc
    19801 tgagcagatt ggacaaaagc tctcttatca ccatgatgaa gtagtcatta cagtggaacc
    19861 ctttatgcta gaaaaaaatg gagttttggc taatggtgta ttggcagaaa ttctgaccca
    19921 catgacccaa gatttagttg ttaatagtgg tcgcaatctc attatcgagc agatgctgat
    19981 ctactttttg caggccgttc agatagatga tatattgcgc attcaggcac ggattattca
    20041 tcatacgaga cggtcagcta taattgatta cgatatttat catggtcacc agattgtttc
    20101 aaaagcaaat gtgactgtta aaattaatta gaaactagga gaaaagatga taacattaaa
    20161 atcagctcgt gaaatcgaag ctatggacaa ggctggtgat tttctagcaa gtattcatat
    20221 aggcttacgt gatttgatta agccaggcgt agatatgtgg gaagttgaag aatatgtccg
    20281 ccgtcgttgt aaagaagaaa atttccttcc acttcagatt ggggttgacg gtgccatgat
    20341 ggactatcct tatgctacct gttgctctct taacgatgaa gtggctcacg ctttccctcg
    20401 tcattatatc ttgaaagatg gtgatttgct caaagttgat atggttttgg gaggtcccat
    20461 tgctaaatct gacctaaatg tctcaaaatt aaacttcaac aatgttgaac aaatgaaaaa
    20521 atacactcag agttattctg gtggtttagc agactcatgt tgggcttatg ctgttggtac
    20581 accgtccgaa gaagtcaaaa acttgatgga tgtaaccaaa gaagctatgt acaagggtat
    20641 tgagcaagct gttgttggaa atcgtatcgg tgatatcggt gcggctattc aagaatacgc
    20701 tgaaagtcgt ggttacggtg tagtgcgtga tttggttggt catggtgttg gcccaactat
    20761 gcacgaagaa ccaatggttc ctaactatgg tattgcaggt cgtggactcc gtcttcgtga
    20821 aggaatggtc ttaaccattg aaccaatgat caatacaggc gattgggaaa ttgatacaga
    20881 tatgaaaact ggttgggcgc ataagaccat tgacggtgga ttgtcatgtc agtatgaaca
    20941 ccaatttgtc attacgaaag atggacctgt tatcttgact agccaaggtg aagaaggaac
    21001 ttattaataa aaagtgaaaa gactactgga agtttattat gaaaaaatcc agtagatctt
    21061 ttcataataa aacgcattgt atcaagtgtt aggggctgat atcatgcgtt tttctgcttt
    21121 taagattttt tccaactctg tttgtaagcg catcataaca aagggtctag gattcagggc
    21181 tctcctccta tatactatta gtaaagtaaa actaagggag gatattttag tgtcgcagtc
    21241 tattgttcct gtagagattc cacaatattg tcgttttgat tctaaaaaga gaaatggaat
    21301 tctgtttaat gttcgtattg ccaatcttaa atttaccttt tttagattag acttcctgcg
    21361 aaacaaaaca tggtatagta gttctatgaa tgatgaagca agtaaacaac taactgatgc
    21421 acgatttaag cgtcttgttg gtgttcaacg cacgactttt
//
LOCUS       DAVBCV010000035        21373 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBCV010000035 DAVBCV010000000
VERSION     DAVBCV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21373
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.035"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(450..2177)
                     /gene="ezrA"
                     /locus_tag="VQ516_001300"
     CDS             complement(450..2177)
                     /gene="ezrA"
                     /locus_tag="VQ516_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2511737.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2259..4205)
                     /gene="gyrB"
                     /locus_tag="VQ516_001301"
     CDS             complement(2259..4205)
                     /gene="gyrB"
                     /locus_tag="VQ516_001301"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2511738.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4220..4783)
                     /locus_tag="VQ516_001302"
     CDS             complement(4220..4783)
                     /locus_tag="VQ516_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2511739.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4868..5422)
                     /locus_tag="VQ516_001303"
     CDS             complement(4868..5422)
                     /locus_tag="VQ516_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2511740.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5433..6656)
                     /locus_tag="VQ516_001304"
     CDS             complement(5433..6656)
                     /locus_tag="VQ516_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2511741.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6693..9143)
                     /locus_tag="VQ516_001305"
     CDS             complement(6693..9143)
                     /locus_tag="VQ516_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2511742.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEDKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQAMGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9342..10271)
                     /locus_tag="VQ516_001306"
     CDS             complement(9342..10271)
                     /locus_tag="VQ516_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2511743.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10411..10698
                     /locus_tag="VQ516_001307"
     CDS             10411..10698
                     /locus_tag="VQ516_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2511744.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10732..12066)
                     /gene="ciaH"
                     /locus_tag="VQ516_001308"
     CDS             complement(10732..12066)
                     /gene="ciaH"
                     /locus_tag="VQ516_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2511745.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12056..12730)
                     /gene="ciaR"
                     /locus_tag="VQ516_001309"
     CDS             complement(12056..12730)
                     /gene="ciaR"
                     /locus_tag="VQ516_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2511746.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12839..15385)
                     /locus_tag="VQ516_001310"
     CDS             complement(12839..15385)
                     /locus_tag="VQ516_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2511747.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHIAYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15509..15949)
                     /locus_tag="VQ516_001311"
     CDS             complement(15509..15949)
                     /locus_tag="VQ516_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2511748.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15924..16793)
                     /locus_tag="VQ516_001312"
     CDS             complement(15924..16793)
                     /locus_tag="VQ516_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2511749.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16835..17563)
                     /locus_tag="VQ516_001313"
     CDS             complement(16835..17563)
                     /locus_tag="VQ516_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511750.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17571..18050)
                     /locus_tag="VQ516_001314"
     CDS             complement(17571..18050)
                     /locus_tag="VQ516_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2511751.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVDHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(18061..18759)
                     /locus_tag="VQ516_001315"
     CDS             complement(18061..18759)
                     /locus_tag="VQ516_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2511752.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18752..19018)
                     /locus_tag="VQ516_001316"
     CDS             complement(18752..19018)
                     /locus_tag="VQ516_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2511753.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(19084..20010)
                     /locus_tag="VQ516_001317"
     CDS             complement(19084..20010)
                     /locus_tag="VQ516_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2511754.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20085..20900)
                     /locus_tag="VQ516_001318"
     CDS             complement(20085..20900)
                     /locus_tag="VQ516_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511755.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20904..21224)
                     /locus_tag="VQ516_001319"
     CDS             complement(20904..21224)
                     /locus_tag="VQ516_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2511756.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6465 a         4684 c         3749 g         6475 t
ORIGIN      
        1 gaaaactctc gtaaacaaag aggttttaga ggcctattta ccgtggacta aagttgtaca
       61 agaaaagtgc aaataagaaa tctccagatt aggaactatc cgtgagttct ctagtctgga
      121 gatttttcaa tagacttcgt tattggacgg ttaccaagtt acttcaaagt acagatttga
      181 agctgcagat aagactgacg aaatcacttt aatactaaat gaaaatcaaa aagcaaacta
      241 agaagctagc ctcaggctgt acttgagtac ggtaaggcga cgctgacgtg atttgaagag
      301 attttcgaag agtataaaac actgttttga agtggtggat aaatcggctg ggtaaaaact
      361 caatctcagg agaaaatgaa gtaaatctcc ccacaactat agaaaaagat ttgattctgc
      421 tcctcacaca ataaaatctt tttcttttat taaaaacgaa tcgtttcacg tgttttctca
      481 tatgaggtaa caaagcgatt ggttacacca ggctctgcca cttccaatgc ttgagaaatc
      541 ttgtcaaatg aagcgtgata atcaaattct ttttcaaaaa tatctaaagc ttcgttaaat
      601 gcttcttgaa tgcgttcatc aaatgagcga tagcggttag aatattgcaa gagttgctct
      661 gtcaaagttg catattgtac aatattataa gtttccgttt ctaaagcttc catatcattc
      721 gttgcaattt caagaactcg ggtaacagat tcaatgttaa tcattttttg ttctaactca
      781 accattaaat cctcggtatt attgcttgcc gtaaagaata acttcaagaa agtttgtgga
      841 atacctggca gattgcgttt ttccatgtat cgcttgatag tatggagacg attgacataa
      901 acattggcct tttgacgtgc attaatatca tctttctcaa tttgtgtcag gcgctcacta
      961 actgaaattt gctcatcttc aatatctttt agttgagttt gtaaatcctc aagattttct
     1021 tcaagaactg aataagcttg ggttggttct tcttgatttg aagttacctc aacaatagct
     1081 gcctcaaaac tctctaattc tgtctgaata cgacgaacat ggcttgcagc tgtctcagga
     1141 agtaaatagg tcttgttcaa acgtgcaata tcttctccca ataaagtatt attctctttc
     1201 atatgttgaa ggtaagttgg aagagttgca agtagatttt ccactacttt ctgagcagca
     1261 atttctcgag taaaaatatc atacaaggca ttgatttcct cttgtgcctg tccattctca
     1321 tattcggcat tatccaattc caactgacga atattctctt ggtttttctt gaatgcttca
     1381 taaagcaagt ggaaacgcgc ttcaatatcc gtttcaacaa aatgataatt agcatcaatt
     1441 agtttacgat aaccggcttc caaatcctgt aattgatctg gcaattctgt agaaagcgtc
     1501 gtaaccaagg ctggaacacg atccacaata tgacttaagg ccaaaatgtg attttctgta
     1561 ttatccaaaa tcactgcggc ttccacaggg tcacccgatg aattcaaggt tacaaattgt
     1621 gaaaattcag attggatatt ttctaattgt ttttcaattt catccaaggc ttgaccatac
     1681 tgttctgaat tttcagcaac tctatgctga agttcctcaa ataaatccaa agcatgaaga
     1741 acacgaccac tatttttaga ttcttgcttc tctaagtctg ccaaagcatt gcgaattgcc
     1801 gcaatatctt cttcaatcaa agtaatttga ctctcaattt ggtcaatttg atgactggcc
     1861 ttgagaaaac gaaatgaatg gttataacct tctgcttcaa agagattatt ttcaatatcg
     1921 gcaaaagagt tgagagataa atcgacccat ttttgattcc attcacggaa agccacttga
     1981 ctttgtccaa tcaagtgcat attttttaca gcttctactt catcatttac tggaagattg
     2041 tatagttctt cttttctttc ttctagcgcc tctaatctcc cctcgtttcg cttacgtaga
     2101 aagattgcca ctacataagc cagaactaaa atgactgcaa ttgcaaccat taaataaatt
     2161 agttgtccat tagacatatc aaactccttt tttacttgaa acaatcgtaa cgattatatc
     2221 atatttttta gaccaaggga actacttctc cctatttttt agacatcaag tgtactatag
     2281 acagcatttt cttcgataaa ctcacgacga ggctctactc gatcccccat caacatatca
     2341 aagattttat ctgcttctgc agcatcatct acagaaactc tagccatcaa gcgatgttcg
     2401 ggatccatgg ttgtttccca cagctgatga tcgtccattt cacctagccc cttataacgc
     2461 tgaatagtcg gtttggtacg accttcacta taacgggcta aagcttcttg gagtttgatt
     2521 tcttgatctg cacccggctg gatatattct ttaatctcgc ttccaacctt gacaccatag
     2581 attggtggtt gggcaatata aacataacca gcttctagga ttggtttcat ataacgataa
     2641 atcaaggtta aaagaagggt acgaatatgg gctccatcga catcggcatc ggtcatcaaa
     2701 acgagttttt ggtaacgggc tttcgaaaca tcaaattctg cgccaaatcc tgttcccatg
     2761 gctgtgaaaa gactacgaat ttcttcgtta gctagaatct tatccatact tgctttttca
     2821 acgttcaaaa tcttaccgcg aattggaagg atagcctgaa actcacggtt acgaccagat
     2881 ttggctgatc caccagctga gtctccttcg acgatgaaga gttctgtttc agcagggtta
     2941 ttagaagaac agtctgctag tttccctgga aggttggaaa tttccaaacc agatttttta
     3001 cgtgtgactt cacgcgcacg cttggcagcc acacgagcct tggcagctaa aatccctttt
     3061 tccacgatac gtttggcaat ctgtggattt tccatgagga aatcggagaa ggcttcactg
     3121 aagaggcgat tggtaatctt gaccacttcg ctatttccca atttggtctt ggtttgtcct
     3181 tcaaactgtg gatttgggtg tttaactgag ataactgcag ttaatccttc gcgaacatct
     3241 tcccctgtta aattatcttc attgtctttc agtaacttat tcttacgagc ataatcattg
     3301 ataacacgag tcagtgccgt acggaaacct tgttcatgcg ttccaccttc atgggtatga
     3361 atattattgg cgaaactcat gacattttca tggtaacctg ttgtgtactg catggctacc
     3421 tcaactgtga tatcatccat ctcaccgtct gtatagattg gtgtatcaaa gattacatcc
     3481 ttgttctcgt tgatatattc aacgtaacta gcaatcccac cttcataatg ataatgcttg
     3541 gtttgttcca aaccttggcg cttatcagtg atagaaattt gaagaccgcg atttagaaag
     3601 gccaactctt gaatccgttt atttaattta tcaaaatcaa aggttgttgt ttcagtaaag
     3661 atttttgggt ccggtatgaa gtgaaccgtt gttccagttc tatctgtatc cccaaccacc
     3721 tcaaggtctg caacaacatg accacgacgg tattcttggt aatgaatctt accgttttta
     3781 tggacatgaa cgtctaattg agtggaaagg gcattaacta ctgacgaccc caccccgtga
     3841 agaccacctg aaaccttgta tccaccaccg ccgaactttc ctccagcatg aaggactgta
     3901 aagacggtct caacagcagg acggcctgtt ttttcctgaa tatcgactgg gataccacgc
     3961 ccatcatcca caacagtaat cgaatcatct ggctcaataa aaacttgaat atggctggca
     4021 aatcctgcca aggcctcgtc aattgagtta tcaacaattt cccagactag atggtgaaga
     4081 ccttcttttg aggttgatcc aatgtacatc cctggacgca tacgaacagc ctctaagccc
     4141 tctaaaactt gaatttgact ggcatcataa tcctgtgcct gcagattttt gatttcttct
     4201 gtcatcttat tcctttttct tatatgtcta atacttgtct taaattcacg atactggtaa
     4261 acaagtgggt atcaagtcct gcagcttgac ctgcttcgat atcaatcggc cgatcaccaa
     4321 tgacaagacc agagctaatc tgatactttt ctcttaaata aagcatggat tcgggatttg
     4381 gctttctctt aaagcctgag ctagaagtca ccacttctgt aaaataagct gctatagagg
     4441 ttttttctaa aatttccaaa acctgatcat ttcgatgaga gaccaaaaaa tgacggccac
     4501 cttgatttaa aatgtcttcc aataggtcag aaactccttc aaataaaatc gggtgttcaa
     4561 gctctctggc ttcattttcc ttgtactttt ctaaaaaatt ctctaaattg ggagcgaatg
     4621 tctcaatcgc aaaaggagta gaaaccttta aagcttgata gacactgtca tggtcttgtg
     4681 tgataccata cagtgccaat gtttcaacaa atgcagctgt tgaagtttca taattatcca
     4741 gtaaagttcc acctaaatcc cagatgtaat cgtgatattt catacccttc attatagcat
     4801 ttttttatga aaaaagcgat gatttccctg agttttccac ataaaaaacg agagaataac
     4861 tcccgtttta ctgattttta ccaaagacat ctcgatagag cattcctgtt cgtaaactct
     4921 ctgcgtcccc tcctagttgc tccaccaact cgtaggcaaa ggcaagggct gttgaaggac
     4981 cccgactggt tgtcaactga ccatctacca ctactgtttc cttgacgtag tgaccatcaa
     5041 ggatttgctc ttgaacgccg tcataacaag tgtatcgctt atttttcaat atctctgctt
     5101 gattgagggc aattggtgcc gcacaaatgg ctgctagttt cttcccttct tgctcgaagc
     5161 tttgcaattc ttgaatcaag gtctgattat cacgtaaatg tgcagaacca ggcatacctc
     5221 caggaagaac aatcatatca tagtctgata aatctccatc aaagacatga tctgctctta
     5281 cttggattgc atgcgaaccc gttacttgct cttcaaaacc aaccatatca catgtgatat
     5341 tggctcgacg caagacatct acaactgtca aggcttcaat ttcttcaaag ccctgagcta
     5401 acataactgc tacttttacc atgattttct ccttatttaa tttgttttaa tacaaccttt
     5461 ttccgtttga atgggacttt tccgctcttt tcttcagcta gattagtctg gtaactcatc
     5521 gataaaagca aaccaacacc aatcagatta ctgataatag ctgatccccc ttgcgaaatg
     5581 aaaggcaagg gaatccccgt caaaggaagt agtccagtca cagcaccgat attctcaaag
     5641 atgtggaaga gcaacatcat aatcaaacct gtggaaatat aagtgtagaa ctggttattt
     5701 gatttaagag taatcttcaa catacggtaa atcaacatga gatagagggc aataaccagg
     5761 acagagccaa taaagccaaa atcttctgca ataaccgtaa aaatcatatc tgactctcga
     5821 actgggataa gcagattcga agcattaaat ccctgaccaa ataagccacc actcccaatg
     5881 gcaatctgcc cttgagcctg ctggtaagtc gttgtttggg caaactcaaa gggattgagc
     5941 caagccaaaa tccgattgat ttggtaggtc ggcattccaa tctggtgaag aaaagctcgt
     6001 ccgtccttgc taataaagat agctaagaaa ccagcaactc ctgttacagc agtcacaaat
     6061 actgggataa taattttcca agaaacccct gataataaaa cgattcctga gaaaatggct
     6121 acaaaaacca aagccgtccc caagtcactt tgaagtgcta aaagaactag gactggaatg
     6181 gtaaagagaa tcatccagaa aattaacaaa aagtccagcg aaaccgtgcg tctccattcc
     6241 ttatgtttct ttgtaaattg gacaatgaca cgagccaaca tgaggatata ggatatcttc
     6301 ataaattctg acggttgaaa tagggtaatt ccatttattg atacccagtt tttggcaccc
     6361 gttgatgcaa ctaagcttgg attataaaat acaatcggca agatcataag tcccaagcct
     6421 aaaatatata gaaagggggt caccttccaa agaaattctg tattaaagag catgaccaca
     6481 aaaccaatca caagccccaa ggcaatccag gcgacctgct gccctaaaat gggcagaata
     6541 ttattgggat aatcatgact aacggctata tagatagcca ccacaccgat gaccagtaga
     6601 aaaaatactg gcaagagcaa actataatcg actctagagt cgagagaacg tttcatatat
     6661 actaacctta tactttcata caatactatt tatcaaagtt cattaaaaaa tctatcaata
     6721 gcctcgtcaa cttcggatcg agagatggtt ttaacagtcg cttcttctgc tagagatgct
     6781 actatttgtt tgccgtatcg ttttccgatg attctcctat ccaaaataag agttaaggaa
     6841 cgttggtatt cacgtctcat acttcttccc aaagcctgtt ttaaacgaat aatggccatt
     6901 ggcaattgat aatcataaaa ggcatttttc ccttcttgat tcagttcttg attaatcttt
     6961 ttcgtcaagg gttcttgagg attttggaaa ggaagcctcg gtacaacttg aatcacaaaa
     7021 ggatggcttg aaaaatcaac tccctcccag aaacttgctg caccaagcaa gatttgttgt
     7081 tcaccttttt caaagcgttt ctttagctga tgaacatccc catttttata ctgggccaag
     7141 tggctaactg taagtaaatc cgatactgct agaagcatgt ctttagcggt aaagagaacc
     7201 aaaatcggtt gctggaaagc ttgaatttcc actagtaaag cagctacctc tctggcatag
     7261 acttctaagg aggtttctgt taccagaggg aaatctttga ccataaccac ttcttgttcc
     7321 tgttttcccc gagattcaat cttgacaaat ttggcttcag gatagcctaa aaggtctgcc
     7381 aaagaaaccc tctgactaat ctcaagagta gccgatactc ccatggcttg gcaactctca
     7441 ggaagtaaag aggacagaag agtacggcct gattttgtag aagaaatctg aattttcttt
     7501 tgactcgttt cagttacttc aagccagtaa tcaccttcag ctgtaaaata gcgaaccagc
     7561 tcatcaaagc cttctacttc taattctgaa aaatactgat ggagattgtc cagagaatct
     7621 aagatatttt tcctagattt accagactga aattgttcta tcaagtagag acactcaaag
     7681 cgaatacttt ctagtatccg ttgttgaacc ctgttttctt ctcctactaa agccttatca
     7741 attaaatcga taatagattg tatatcgtag gtctcttgaa gcagattttc tagagctaac
     7801 aaaatctttt ggacttcatc aataatcagt aaacggtcac tgacaaattc aggattatct
     7861 tcaagtctgg tcacgagata ggcatgatta gtcactaaaa gcttgcaagt ctctgccctt
     7921 tcttgactac gtttccaaaa atcttccgtc acaaataagc tctgggatga taaattccca
     7981 tcatgacgaa ggtctgctag aaaatgttgg taacgataga gttgcccgat ttcatccaaa
     8041 tctcctgtct ctgtctcagt aagccagacc aagacttgca ttttaaagcg tctaaataag
     8101 cgattttcat cattttcctg caaggaatga taaaaggcat ccaacttcag ataattttgt
     8161 ggtcccttta agctatgaat atctgtatgg aacacttcct tgaggcgttt atcttcttct
     8221 tccatgattt gattttgaag aatctttgtc ggaacactaa gaacaatttg tcgctctttg
     8281 gattgagata aagcgggtaa gagatagcca taggttttcc caatccctgt cggcgcttga
     8341 atcagagaga caggttcatc tttcaatagc aagccaacct ctttagcaaa actttcttgt
     8401 tcctccctca cttcaaggtt caacagagaa atatttttag aaaagtcttg agatagtttt
     8461 cgtggctcca gagaagctgc cgttttctta aaatacagac cttgaacttg gaccaagtct
     8521 ggagaactca ggatagattg gttgcgataa gtttcctcaa taaccaggta ggactcatat
     8581 aggagagcgt cagccatttc cagcaagcgt tccaagagac ctttaggaag ctgagtcatc
     8641 ttttcccgta aaaaaagaag taattctgct gtagcttggg catctgaaag ggctgtgtgt
     8701 gcgtgtttaa gaggaattcc taattctcga cacaaaatcg gcaagctata tttttccagt
     8761 tcagggaaaa agacctgggc caattcgacc gtatcaacac gagggtttct tagctcatag
     8821 ccttcaaaaa ataaattttc cgccaagaga ttagcatcaa actgaacatt atgggctaca
     8881 aaaatcccat cctccaccaa gtcaaatatt tttctggcaa cttgcgaaaa atcaggtgct
     8941 tgcgccagac gttggtctgt caatcctgtc agttctttga tatgagcatc caagggttca
     9001 tgtggattga catccgtcgt atagtgatcg acgatttctc cgtcctcaat cacgacaatt
     9061 cccacttgga taattttagc cttactacct gtgctagttg cctctaaatc caccacaaca
     9121 tagcgattac caatcgtttt cattataaaa aattatacca aaaaaagggc catttggcac
     9181 ctgcaaacat aggataattt tcaaactcaa gaacactact tttcttaaaa atcccatcga
     9241 atagagcatt ctagcctgct gattttttaa aatagtgaag aaatacgaaa aattgagaga
     9301 agaaatgttt tcatcttcct tctctcaacg aatcatgtat tttatttaac catgtcagga
     9361 tcgtagtcat aaaatcctgt atcaaggaaa cggtttttag ggtgaagttt acgaacttct
     9421 tcatccagca agaaggcttg gtcatggcta aagttgccct cttgaatcaa gctacgcgct
     9481 tggataaaga gttttgcaat ctcaacaaag ttctgaccag gagtgtagag cccttctagt
     9541 ttccagcgag taaagccatg ctccaccaat tctgttaatt tgatcatcaa atcaaggtca
     9601 ttgttggcaa agatatgggt cccatgatta tcttcaaaaa tggaatagtg gctctctgga
     9661 tcacttggct cagccaagaa gaggtcacgt ttatgcgtct tttcatcatc gatatgtgta
     9721 aagttatagt agttttgcaa gagtggacgt ttagaatgat ggatgacgct agcaccgtaa
     9781 accaaaactt cagcaggaat ttccaaaatc tctggcattt tgaaaagttc agctgatgga
     9841 atttcacgcg ccaaaacagc ctcagatgcg ccagcctttt gtccccagaa gttaatctga
     9901 cgactgctag ttaccatggt tgaagcatcg tagatggtct taaatgaata accatcgcgg
     9961 ttaactacgt aaaagacgcc tgcatcccca atcgtaatat agtctgtctt gatttcttcc
    10021 aagaagtcta agaaaggctt gatacggtcc atcatatctt ggtgcatgag agcattgacc
    10081 gcaacgagca attccttacc agcatcatga accaacttag cgatttcacg taattggtca
    10141 taactaaagg tcgttggcag acgaaggcca aaatctttct caccgacata gatacggtct
    10201 acgccagctt cgagtagttg ttcaacttgt tcaatacttt cagcagttgc tgtaatgaca
    10261 atcttttcca taagaaaaat tataccacat ttctccaaaa tcatccatgc tttgaaaatg
    10321 aaaagggact ttattctttt tgtaaccttt ctgtaataat tctctgccga aaaaatgata
    10381 aaataggtat gtactgttaa ggagagaatt atgcccgtaa gaaaattaca atcctatgag
    10441 gtagactatc aagaagaatt aaaccagcag cttcctcatt accaagctta tacacctgaa
    10501 gcgcaatctg atgccaatct caaggaaatc ctatttttta ttaatatcgc tgttttttgt
    10561 atctgtattg ctatctttag ttttatcttt ttggcattaa aattatcaac tgctcttgcc
    10621 tttgccgcag caatcggatt cagcttactt gttttaaaag ttcaacggtc tattatcaaa
    10681 cgaaaacgta gaagataaat ccaaaatatc gccccagttg gagcgatatt tttatttttt
    10741 ctttttagat ggtgtctgaa tggcaatctt cacttcaaag attgttccct tgggtttatt
    10801 atctttgaca gtaacagttc cttttagagc atctacaatt tgcttggcta gggataatcc
    10861 taaaccaaaa ccaccttttt gccgagttct agccttgtct actcgataaa aacggtcaaa
    10921 aattttcttt ttatcttctg tcgaaatacc gattccatta tcagaaacaa gtaaataaag
    10981 attgcgatcg gtcgccgaga taagaaaatc aatttcacca tcctcctcag tatacttgac
    11041 ggcattatcg aaaagaatgg tcatcagttg tttcagaaga agctgatctg tgacaattgt
    11101 tcgatggata cgattttcaa aacggaagac acgattattt tccgaagcaa tcatctcgta
    11161 gtttgtgaaa gttgtattaa aaaagctagt tggaacttct gcaagctccg gcttaatccc
    11221 atcatctctc cgagctaagt tcagcaagct tgtcgttaaa aaacgcatat ttcggacttc
    11281 ttccaaactc gatgcaatgc tttcgctcac atccataatg gtagcttctg gcttacgaaa
    11341 aagggtctct aagcgatttt gcaaaactgc gagtggagtt cgtaactcat gactggcatt
    11401 ttccacaaaa gactgttgct tctgcatact ctcaagcagg ggcctaacac tgaccctagc
    11461 tagatagaga ctggcaagta aagacaaaat ccagaaacta gccatcacga ccacaatcaa
    11521 ttgctcatgc ttttgactag cctgttccaa ctgactggta ttaatcaaga cagcagcata
    11581 cttgatattg gttgaaaccg aactaatatt ggtctccatc aaaatcacac gatagatttc
    11641 ttcctgccca tagctattaa aaacctgaat ctggtagata tgtcctagtt ctttcttttc
    11701 taacttaatc ttatccaagc ccaaaaatcg atttccagaa agaagttgag taaagtcttt
    11761 atcaaagaga atgacttccg tattggaact gacattaggt tttatttcta ctttactagc
    11821 gtccgcccta gcattttcta aatctttaat ctcttctgtt gccctattta tagccagctg
    11881 aataactgct tgaggatttt cactcagtcc atgaagctta tcgtccaccg aagtataaag
    11941 actcgaatgc atgacttgta aaataatcag agtcattgta gaaaaaatca gggtgaagac
    12001 accgaagttg cggataaaat aactaaagtc atccgcatac catgtttttt taagtttact
    12061 gaacatcttt taaaagatac ccaacactac gcaaagtttg caaattctct gcaaaagtgg
    12121 ttccctttaa tttcttacgg acttttgaaa catagacttc gacaaccgaa atcgttgtat
    12181 cactatcaaa tccccataga cggtcaaaaa tctgcgtctt aggcaaaatc acattttgat
    12241 tttgaaggaa ataaactagt aaatcgaact ctttccccag caattcgaca ggagtatctt
    12301 caactttaac ggtattggtt gataaattaa ccacgatatt cccataagtc aaggtgtttt
    12361 cattaaactt ccctgaacgt ttgagaaggg cctgaatccg cattttaagt tcttctaggt
    12421 agaaaggttt ggtcagataa tcatccgctc ccagttcaaa tccatgtccc ttgtcatcca
    12481 aactttcctt ggcagtcata atcagaactg gtgtcgtaat tcccttttca cgcaattctt
    12541 ttaagacttg gaaaccattt ttttctggca acatcaaatc cagcaaaatc aagtcataga
    12601 caccactctc agcttcgtag agaccttctt ctccatcaaa tacctgcata acatccgcaa
    12661 aatcgtctaa aaagtcaaat actgaatttg acagacctag gtcatcctca accaataaga
    12721 tttttatcat gagaaactcc tccttattaa aactattata ccaaatttgc cttaaaaaaa
    12781 actcaactct ctgcatttta catgagatag ctgagttttc tttttatttt aggcttattt
    12841 atgcatttcc gtattgaaga acaactgctt cgactgcagc tttttcacgg ctaatcaagt
    12901 caacacgcgc tgcaatttcc ttgattccca taccgatgtt acggctaaga gcaaggtcag
    12961 aaagttgcgg ctcaaagaac tccttgtatt ccgccaagcg ttgctgagtc ttaaatacat
    13021 gagcaggaag gataacaaag ctatcaaagc tcatatctcc tccaagggct gccttaatcc
    13081 aagcccagtt ttcacgcgcc caagaccaag ctgttttctg agttgcttga tgagctagga
    13141 attggtaata ccaagcagac aagtcctgtg gtttgaccac aaatttgtcc ttccaagaag
    13201 taatcaagtt ttggatatta tccgcatctg tactgtatgc aagagctgct gccaactggc
    13261 gtttaaagac agcatctgtt gcgtgagtat aagtatcaag ataaagtgct aacaagtctt
    13321 tagtctcatg atgtttcatc tcattaatca gaacttgtga acgaatagct gctgggagtc
    13381 ctgcaagatt ctccttgtgt gttgcgaaga tttggctagc gacttgacta gcttctgcat
    13441 catttgagcg aatcatcatc gaaacagcca gctgacgaac caattcatcc tcatctgatt
    13501 ctccgtcttt agcttcaaaa ccaagacggt catagttatg acgagccaat ttagcaacca
    13561 gtcctttgaa ggctgtttca gcatccgttc cttcatcaat aaagcgctca agggctgaaa
    13621 tcacttgaga aacagctgaa accaccagat aagactcttc cttagcaagt ttatcaagaa
    13681 ctggaagcaa gtctgcataa gcaatgtgcc ctgcctcagc caacaaacga cgttcttgaa
    13741 caatttgcag tttgcttgtg ttatcaagtg tctctagctc agcaagaaca gcttctaaca
    13801 agtctccttg atagtcggta atatagtggg cagtattttc agtgttgaga cgaagagctc
    13861 cttcattttc agcaagaaga gctgcgtagc cagggatttc gatactttca gtttcgagtg
    13921 tatcaggcaa gcctttccag ttgctattga ggggcaccac ccagagacgg ttcttgtctt
    13981 cgttctcacc gatgaagaat tgtttttgtg aaatcttcaa gacatcattt tcaactttaa
    14041 cagtaagaac tgggtaacca ggctgttcca accaagaatc catgaaggct gcgacatcac
    14101 gtcctgacgc ttgaccaagg gcatcccaaa ggtcactacc aatggtgttg ctgtattggt
    14161 gtttttcaaa gtaggcgtgc aaacctttag caaaatcagc atctcctagc caacggcgaa
    14221 gcatgtgcat gagacggctt cctttggcat agacgatagc gccgtcaaag agtgtattga
    14281 tttcatctgg atgtttaact tcgacgtgga cagactgaac gccatcagta gcgtcacgtt
    14341 caagagcaag aggtactcca cctgtttgga aatcttcaaa gatattccag cttggttcga
    14401 tggtatccac acagacgtat tccatcatat tagcgaaact ttcattgagc caaaggtcat
    14461 cccaccattt catagtcacg aggttcccaa accattggtg agccaattca tgggccacaa
    14521 caagggcaac ttgttgacgg ctagcaaatg tagagttctc atcgacaacc aagtaaactt
    14581 cacggtaggt cacaagaccc cagttttcca tagcaccagc tgagaagtca ggaagggcga
    14641 tgtggagaga ttgaggaatt gggtacttaa ctccatagta atcttcgtaa aactcgatag
    14701 agcgaacagc gatatccagt gagaaatcaa gatttgaaag tggatgtgct ttggttgagt
    14761 agacacctac cagggtacca tttttagttt tagcggtcac cccttgcaaa tcaccagcaa
    14821 caaaggccaa caagtaagaa gacatgcgag gtgttgtctc aaacttccag atacctgttt
    14881 ccttacggtt ttcaacatcg atttctggca tgtttgacaa ggccaattca ccttctgctt
    14941 ggtcaaagcg aagagagagg tcaaaagttg ctttggcttc aggctcatcc acacatggga
    15001 aagcttcgcg cgcaaaatgg ctctcgaact gagtagacaa gacctccttc ttgactccat
    15061 caactgtata ataagaaggg taaatccctg tcatgttgtc tgtaatttta ccagaaaagg
    15121 caagaaccaa ttcaacttga ccagcctcag ccaattcgat atgaagggct tcattgtcat
    15181 ggtcaactgt aaatggacga gcttgacctg caacttctac agaggtgatt tccaagtctt
    15241 tttggtggag ggagatgcgg tcactctgtg cttgaccagt gatggtcact ttcccagaaa
    15301 aagtcttggt ctcacgactc aaatctaaaa ataaatcata atgttcagga acaaattgct
    15361 taataaaatg ttcaactgct tgcatagttt tctcctattc taagtttaag agctactgct
    15421 cttcttcaaa caaacttatt atatcatatt ttgcttgaga aaaaaggatt ggacagcgat
    15481 ttccaaaact ttcttccttc taaatagtct acagtgggta gaccttgcga aattcttcta
    15541 aaacaacctt gctttcaggt gtaaaagtca gccctacttc acccaaacag tctgtgaaaa
    15601 agtcctcatg aacctggcgc caataggcaa gtgatttgtc tccctcacct tccttaaagg
    15661 catgttgcgc agaaacttga ttgaagagtt caacagaaac ctttgtaatt tcgacaatgc
    15721 agacagactg attttgacta tctaaaatga catcgaaggt ccctacttgg ggaagtggtt
    15781 cgtcttctag tacatagagg tcataggctg atgctgttgc tgtcttttct cctttaaaca
    15841 ccaaatccgc taaaaggtct ggttcaactc caaaagccca agcatcgatt tcatctccga
    15901 tcaaaggatt gatttgcttg tatttattcc acatttcttg cggtatcatg ggtactcctt
    15961 tgtaattttt tactttcttc ttttatgtgt ttaagatgat ctggatggtc aatctctaaa
    16021 tcaaaaatct ctggaataga actgtagtgg ataatgcact tgatacccaa ctgattcatt
    16081 ttttgtatga aagaagtatt cagatagcct gctacagcaa aattaatctt gttctttctt
    16141 gctttatcct gcatatctct tagcatatct aacattattg gactttccag atcatgccat
    16201 tgactgtttc tcatagtcgc aaaaacaaag gaagtcaaat cattcattcc aactacaatc
    16261 tttgaaatgc ccgtttccag tatactagat aagtcaaaat acgctgacgg taattcaatc
    16321 atcgttccga ctttcccagt aaagccatac tgacgcaata ctgtaatagc ttgttttaat
    16381 tggtcggcat cattgacaaa aggaaagata acagatagat tggggttggt ttgataaact
    16441 tctgtaacga catgtgcttc agcctgaaat tcatccaaac acgccagtaa acgcctggtt
    16501 cctctatagc caaacaaggg atgcccttcg tcaaaaaact ctttagtccc cactaaacaa
    16561 ttggcttctg tattcgttaa ttcagtaaaa cgataccaaa cttcttcatc tgagtacagg
    16621 gagcagatag tctctagata atcttttaca aattgctgac aacttgctaa tatgatgttt
    16681 tgattaagct ctctcaacaa atattcccca cgaatcatgc cgacgtggtg aaatagttga
    16741 ggataaattt tttcaagaat tttttcgcca ctaagggcaa gttgatttct catagccatt
    16801 cacctcgtct taaaagatac aatatcatat ataattatat caaaatttcc tctagttcaa
    16861 aagcttcata aggttcagat aactcatatc ctaaaaattc tgctactttt ttggaagcct
    16921 cattatgagc atcccaaagt ggaaacatat ctctatttaa actctctaga atcattgcag
    16981 caccaagctt tttagcaaat ccatttcctt gttcgtttgg tctagttgca acttccactt
    17041 caactgcttt acggtaaact aaccctgagg aaatcccagc aatcagttca ttatttttaa
    17101 gaatcacaaa gccaaatcca ccttttaaaa caaaatcctg ataggactca aaatccccct
    17161 gtaaatcctg tgaccattct tccgtagaaa agcagttata aatatgatta tcaataggca
    17221 caatattgta accttcctct aaatgagtaa ctagattatt taaaaattca acttgaaaat
    17281 ttgccttatc tttaaaagaa tagcgagtaa aagaatttag ccccactttt cgatgcagaa
    17341 aatcttgcca attagtattt tctgaaataa taatcttata atctaaacca tattttctaa
    17401 caaaatcttc ccaaaatctg gaatccaact cccctgctag gtatagaaaa ttcccaacat
    17461 cataaaagct tgttgtccta tcattgtttc ggtagacagt tccaatttga gatttaagac
    17521 catagagaac catatttttc ttccatgttt taaatagttc cattatccct ctacttcacc
    17581 ttccaattca tgtaagaagt cttgtccagt tctggaaatc ctaataattc agacttaacc
    17641 ttcaagacta atggcgatgc attttcttct gtaatctctt gaatatccat ccaaatatat
    17701 ccaagtgaat cattcgcacc atcagacaca gcttccgaag tcgtaacttg aggtgcactc
    17761 tcattcattt caacatcata caaggccatg acatggtcaa ccataaaatt tgttaactct
    17821 tctctgacga aaacatcgta gattcgagga ttagagtagc ttctaacagt aaatcccgtc
    17881 tcttccataa cttctctagt cagcgtttcc gtcagtcctt caccaagttg ctgactgcct
    17941 ccaggtagat cataccgatg ttgataaggg cctctcgttt tttcaatgca gagtaacttc
    18001 ccattttcta agcaaacagc gtagactcca aagtgttttt tgatttccat ccaactcctc
    18061 ctacttcaaa gaccagccac catctattgt caagatttgt ccttgcatgg cgctcgcttt
    18121 tccacttgct aaaaaaagac taagctctgc tatttcctct ggctcaatcc agcgcttgat
    18181 tggagtttca ctagccaccc agtcagccaa accacctggt tcaaaatccg cagcggtcat
    18241 agctgtcttg actgctcctg gagcgatacc aaagacctga atcccagctt cagcatagtc
    18301 tagagccaac tgcttggtga agccagccaa ggcatgcttg gatgaagtat aggcgtgacc
    18361 acctccacct gctaggctag aagcaatgga acacatattg atgatgattc cctttttatt
    18421 ttccagcatt tgtgtcaaat aataccgagt caactctact ggaataatgt agttgatttc
    18481 aaaaatctct tgaatgtcct gcgccgtttg ttccaacagt ggtttgtaat catccaaaac
    18541 tccagcagta ttacacaaaa catccacctg agggcaccag tcaaaaatag gttccaagtc
    18601 caaggtcaaa tctctctgta aaaagtgaaa atcaccctct aagagtggct tttcaccttg
    18661 gtcaactcca taaacttgat agcccttctc taaaaagagg cgagcttgag ccaatccgat
    18721 ccctgaactc actcctgtaa tgagtacacg tttagtcatg cacttctacc caatccgttg
    18781 ccaaaacatc acaaactgtc gggctccaca tggaaaaacc ttctccttcg ccagaaacgt
    18841 tgattaggaa ataaggtgtc atttcaagtg caagcccatt ttgctcgatg gtatcaaaga
    18901 gttggacata gttttccgca cctccccaac cagttcgtac atattttctc ttagccttta
    18961 acccaggcag gatctcttca aatgtcatgt ttttctcctt taattctaca ttcttcattt
    19021 aattatagca aaaaaccgct ttatacggct ttttgaatgt gagttattca aacctgctac
    19081 tacttacggc aaattattcc ctgcagcaag ataaatttca taccattctt ttcttgttaa
    19141 gctaaagttt gccgctcggc taacttctct caagtgctta ggatttgttg tacctacgac
    19201 tgcctgcatt tttgctggat aacgcaatat ccaagaaatg gcaatagttg aagaggttac
    19261 tccatattta atagctaaac gatcaagtac ttgatttaaa gcttgaaatt tctcatttcc
    19321 aacaaaattc cctttaaaat acccgaattg taagacagac catgcttgaa tgaccacatc
    19381 gtgtaattgg caatattcaa aaatgctgcc atctcgcata gctgcttgac tatcttccat
    19441 attaacatga aaagctgatt caaatcctgg agtaaaagcc gcactcaatt gtagctgatt
    19501 aacagctaac ggctgcttga catctttttt aagcaactcc atcatcatag gattttgatt
    19561 agaaactcca aaatctcgaa ctttaccttg tttataaagg agattaaagg cttctgctac
    19621 ttggtcagat tccatcaaag catctggtcg atgaaggagc aagctatcta gatgatcaat
    19681 cttcaatctt tgcaaaatac cgtctactga ttttataata tagtccttag aaaaatcaaa
    19741 ataggtaaat tcttcaatgc gaatgccaca tttggactga atccacatct tttctcttaa
    19801 atctggacga ttttttagga caagacctaa cagttcttca caacgaccac gaccataaat
    19861 atcagccaag tcgaaggcat tgattccaac agaaagtgct gtttctacaa gctcttcaac
    19921 ttcttttaca gacttatctt ttattctcat cattccgaga acaatttctg ataattcttt
    19981 gtcatcttga ccaagagtta tgtatctcat caaatttttc tcctttaatt tctaacattc
    20041 ttcccttcat tataacaaaa aaccgctttg caacggcttt ttgactatac ttcactccat
    20101 tttatcttct taaacccacg gaacaagaca aagattccaa taaagaggac agctaaagga
    20161 ataacttttg taaggaaaac atttgaaatt cccatccact cataataacg gagcagagaa
    20221 cccaccacaa gatgggcaat aatcatactg acaaatggac gaaagaccgc ttctttccaa
    20281 ttccaaatac cgataactag cgaaatcgta aagacagaca aactatccca gggagccgga
    20341 atataaaagg ctccttcttg tatgaagctt gccattccta catatcctaa aacaactaga
    20401 agaactatag tcccaacaac aatgtaagtg ccaattttca ttttaggaga atcttggact
    20461 aaacttcttc gtaaaattgt ggccacaagt ccaaatccaa tcagaaaaat aagaagttgc
    20521 cctaaaaatg tgagcaaatt gactgttaag agaggacctt tagaaaaatc acttagtagt
    20581 tgataataac gtaatacagc caggacaaga attggcgtca aaagggactc tttgatagaa
    20641 ctgcgaggtg ctcccttgag aatctctttc attatttttt taggattctt acctagataa
    20701 tcctctgcac tcatgccatc tcgttctgct tctgagaaat ctagcatcat caaatagatc
    20761 tgctctctga gatagtcttc atcatagaga aatccagcaa gattaaaact ttcccacaac
    20821 tcctcaaaat acttttgatt ctcctcagaa aactcatgta gcaaagcgct tgtttcttcg
    20881 taatacttca ttttcttcat ggtttaaccc ccattcttaa tcccttctac tttttgactc
    20941 aaatcgtccc attgctgcca aaagactgag acacgctctt ctccttcttt cgttaatgaa
    21001 aaatacttcc gatctggacc atctggcgac gggcgcatgt cgcctcttat ccattgattt
    21061 ttttctaact tttgcaacaa aggataaata gttcctggaa cgatagtatc aaatccagcc
    21121 tctcgcaaag tctgaaccaa ctcataacca taccgctctt tttgaccaat catatccaag
    21181 acacaacctt caagaacacc ttttaatagc tgagtttctt tcatcacttc tcccttctaa
    21241 tctattttgt aatacctact agtaacttca cctatagtat attacttcta cactagtttg
    21301 taaagcataa tagttaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccctac
    21361 cgtatgtatg gtt
//
LOCUS       DAVBCV010000036        21000 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBCV010000036 DAVBCV010000000
VERSION     DAVBCV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.036"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..193
                     /locus_tag="VQ516_001320"
     CDS             <1..193
                     /locus_tag="VQ516_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2511757.1"
                     /translation="LKPLTAEDKACNHALSKERSKVENIFAKVKTFKMISTTYRNHRK
                     RFGLRMNLIVGIINHELGF"
     gene            344..820
                     /locus_tag="VQ516_001321"
     CDS             344..820
                     /locus_tag="VQ516_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2511758.1"
                     /translation="MKLSLYSEVEHYHFLSSYSLNDITYTDTPKNIIEKSKKDKYRYP
                     IVILNKDHKVIGFFCLHLNTGPKEYGYYQNDYALIRGFSIHDNFRNQGYGSNALNEIF
                     EFIDSTLKLEINHIILAVNEKNILAQKAYRNSGFSVVKKDLEGKRGKLIIMEKSRN"
     gene            complement(1494..1886)
                     /locus_tag="VQ516_001322"
     CDS             complement(1494..1886)
                     /locus_tag="VQ516_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2511759.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLTRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKTDKKEEVATDSSEKAEKKIRREA"
     gene            complement(1864..2325)
                     /locus_tag="VQ516_001323"
     CDS             complement(1864..2325)
                     /locus_tag="VQ516_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2511760.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGNRLYMKQDDKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(2288..2548)
                     /locus_tag="VQ516_001324"
     CDS             complement(2288..2548)
                     /locus_tag="VQ516_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511761.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(2553..2981)
                     /locus_tag="VQ516_001325"
     CDS             complement(2553..2981)
                     /locus_tag="VQ516_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2511762.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(2950..3276)
                     /locus_tag="VQ516_001326"
     CDS             complement(2950..3276)
                     /locus_tag="VQ516_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET2511763.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            complement(3278..4294)
                     /locus_tag="VQ516_001327"
     CDS             complement(3278..4294)
                     /locus_tag="VQ516_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2511764.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(4242..5183)
                     /locus_tag="VQ516_001328"
     CDS             complement(4242..5183)
                     /locus_tag="VQ516_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2511765.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(5259..5624)
                     /locus_tag="VQ516_001329"
     CDS             complement(5259..5624)
                     /locus_tag="VQ516_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2511766.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(5775..6833)
                     /locus_tag="VQ516_001330"
     CDS             complement(5775..6833)
                     /locus_tag="VQ516_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2511767.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(6996..8147)
                     /gene="nagA"
                     /locus_tag="VQ516_001331"
     CDS             complement(6996..8147)
                     /gene="nagA"
                     /locus_tag="VQ516_001331"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2511768.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(8300..10117)
                     /locus_tag="VQ516_001332"
     CDS             complement(8300..10117)
                     /locus_tag="VQ516_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2511769.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(10218..11360)
                     /gene="tgt"
                     /locus_tag="VQ516_001333"
     CDS             complement(10218..11360)
                     /gene="tgt"
                     /locus_tag="VQ516_001333"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2511770.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAECGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11490..12347
                     /locus_tag="VQ516_001334"
     CDS             11490..12347
                     /locus_tag="VQ516_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2511771.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(12375..13019)
                     /gene="pcp"
                     /locus_tag="VQ516_001335"
     CDS             complement(12375..13019)
                     /gene="pcp"
                     /locus_tag="VQ516_001335"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2511772.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(13126..13494)
                     /locus_tag="VQ516_001336"
     CDS             complement(13126..13494)
                     /locus_tag="VQ516_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2511773.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(13505..13930)
                     /locus_tag="VQ516_001337"
     CDS             complement(13505..13930)
                     /locus_tag="VQ516_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2511774.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(14355..15497)
                     /locus_tag="VQ516_001338"
     CDS             complement(14355..15497)
                     /locus_tag="VQ516_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2511775.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(15663..16283)
                     /locus_tag="VQ516_001339"
     CDS             complement(15663..16283)
                     /locus_tag="VQ516_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2511776.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNTFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(16287..17567)
                     /locus_tag="VQ516_001340"
     CDS             complement(16287..17567)
                     /locus_tag="VQ516_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2511777.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(17628..19112)
                     /locus_tag="VQ516_001341"
     CDS             complement(17628..19112)
                     /locus_tag="VQ516_001341"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2511778.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(19188..20465)
                     /locus_tag="VQ516_001342"
     CDS             complement(19188..20465)
                     /locus_tag="VQ516_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2511779.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(20572..20655)
                     /locus_tag="VQ516_001343"
     tRNA            complement(20572..20655)
                     /locus_tag="VQ516_001343"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20619..20621),aa:Leu,seq:caa)
     gene            complement(20665..20736)
                     /locus_tag="VQ516_001344"
     tRNA            complement(20665..20736)
                     /locus_tag="VQ516_001344"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20702..20704),aa:Gln,seq:ttg)
     gene            complement(20750..20822)
                     /locus_tag="VQ516_001345"
     tRNA            complement(20750..20822)
                     /locus_tag="VQ516_001345"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20787..20789),aa:His,seq:gtg)
     gene            complement(20837..20907)
                     /locus_tag="VQ516_001346"
     tRNA            complement(20837..20907)
                     /locus_tag="VQ516_001346"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20873..20875),aa:Trp,seq:cca)
     gene            complement(20912..20992)
                     /locus_tag="VQ516_001347"
     tRNA            complement(20912..20992)
                     /locus_tag="VQ516_001347"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20956..20958),aa:Tyr,seq:gta)
BASE COUNT         6296 a         4767 c         3700 g         6237 t
ORIGIN      
        1 actcaagccg ctaacagctg aagataaagc ctgtaaccat gcgctatcca aggagagaag
       61 caaggttgag aacatctttg ccaaagtaaa aacgtttaaa atgatttcaa caacctatcg
      121 aaatcatcgt aaacgcttcg gattacgaat gaatttgatt gttggtatta tcaatcatga
      181 actaggattc tagttttgca ggaagtctaa taatgaaaat aaaggactaa agcaataact
      241 ctaaatacat ctgattttta taggatattt cataaaaaaa atctacatac ctagagtcct
      301 atttagttat gtatcttaca taaagagaaa ggaattaatt atcatgaaat tatctttgta
      361 tagtgaagtt gaacattacc attttttaag ttcttattct ttaaatgata ttacatatac
      421 tgatacccca aaaaatatta ttgaaaagtc taaaaaagat aagtatagat acccaatagt
      481 aatacttaat aaagaccata aagtaattgg atttttttgc ctacacctaa acactggtcc
      541 taaagagtat ggctattatc aaaatgatta tgcgcttatt agaggatttt ctattcatga
      601 taatttcaga aatcaaggat atggttctaa tgctctgaat gaaatatttg aattcataga
      661 ctcaacttta aaattagaaa ttaaccatat tatattggct gtaaatgaaa aaaatatatt
      721 ggctcaaaaa gcatatagaa attccggttt ttctgttgtg aaaaaagatt tagaaggaaa
      781 aagggggaaa ttaattatta tggagaaaag cagaaactag taatgatact agtttctgct
      841 tttctctaag tctgctataa atttataaca tgttgaaata agatgtgaat aaattgattg
      901 cagaattcaa accaatttct aacaatgttt tagaaaactc ggcggactat tccagattca
      961 atccactata ttctatctta tttgcatatt ttgagagaaa taatctatat actaaacttt
     1021 tatgtttata attcattcct ttcgtttcac tcaaggcaca agacagaatg aaaaagtggt
     1081 gtgctcttta ttttgtttta taataatagt gagaaaacct atcactacta caaatcacgg
     1141 ggaggtgaat aagtgagtgg tatagccact acctcgcatt tttataggtg tcattctttc
     1201 catcaagcac aagaactgtg actaggagca cgtaaactta taattgaata agcgactcgt
     1261 ttgtgaaagg aaagaatagt caagggatag gcagactcaa atttggtgaa gggaaacccc
     1321 tcatgttaaa agccaaactt caattttaaa aattttaaaa tttgtttcgt attcactact
     1381 ccttgccttt atattggatt gattaagaga acctttcgcg tttctagaaa ctacagtgga
     1441 ctattctgga ttcaacgcat tatagttgaa ttgactagga attctctttc ttttcaggct
     1501 tctcttctga tttttttctc cgctttttcg cttgaatcag tcgctacctc ttccttttta
     1561 tccgttttct cttctttgat tttgactgaa ggaaacagaa actcatattg actcttatcc
     1621 gtacgaacag tcgtcaccaa gcttgttttc ttgttttgat agctcacctg acctagatta
     1681 aaaacctgtt ccccactttc ttgctcggct ttatccttgg ttcgtttagc catggcaaaa
     1741 gcaaccaatt tttctttatt taaagcatag tcttgatagt gggcgatttg tcgggtcaaa
     1801 taaaattgca acaaaagact aaagatggct gctatggtga ctgcgtagag gagaacacct
     1861 gccttaactt tcttcttttt ccacacgata gatgaactcc ctttctaagc ctttttgaaa
     1921 ctggaaacga aaatgaacca gttgattgtc ctctgtaatc tgagctgatt tgaggccata
     1981 aaccataggc tgataccccc gtccactagc atcggtttta cgaaaatcgt ctgactttga
     2041 cttaccgatg gcgatgtcct tgtcatcttg cttcatgtat aggcgattgc cttctacttt
     2101 ttcgaactgc gaacggtcta attctgcctc aagttggccc acaaacaaga gccactcctt
     2161 ttgctcgctt tgttgctggt agcgaacttc tgaaatgagg agctgactca tagcttgaaa
     2221 aaggagtaat cccccactga tgacaatgag ggcaagcagg gattctaaca aggtaaaagc
     2281 cttgacctta tggctctttg attgccaaca actgttctga accatggtag acctccaatc
     2341 ccttttcact agaaaatacc tgaatctcaa ctccgttgat gcttacctga ttttgccccg
     2401 tctgcaaggc catcttagct accctcaaga cttcttcctt ttgcaagatt tttgcttcct
     2461 cttgcctatt tttttgaatt tgtcccaaaa ggagggtcgc aatgctggca aagatagcta
     2521 gagcgactac tgcttccagt aaaatcactg ccctaatttt ttgtttcctt aatgcgttta
     2581 atttttccat ttcctagata taattgatag cgaatcgctc ctttactggt ctgaaattca
     2641 accttagcca gggacgaatt gcccccagct cggtcaaatg taatactttg gcctgatggt
     2701 gcctgaattc ctttaggaac tgtcaacttt tgactgccat tgctaatcat ctgcccatcc
     2761 aagttcagac tagtcttttg ttgactagct acactgcgtt tttgggtttc ccgatagagt
     2821 tcttcaaact ccataaagaa aatctgttcc tctaccgctg caaaagtgga ctggacagag
     2881 ccggacaagc ccaaggcaag gatactcaca agtcccaaaa ccaagagact ttccagcatg
     2941 gtaaaggcct taatcattga ctttacgatt tgcccctcca tttttagtat agtattcatt
     3001 ataagcttta acctgttctt ccgtgatgcg cccatctcct tgtaacttgc tcagagtagc
     3061 atcttcattt ttatccaagc tataaagttc tgcctggctt tccaccacct taacaacagc
     3121 tgcttttcct ttgtcattga ctgcttcttt ttgcttggtc agattaggta caaagagcaa
     3181 gaaaagcacg ctgataatca gcaagaccac caacatctcc accaatgtaa aagctttaac
     3241 cttagctttt ttcaagaatg tcatcatttt tttcatttta aaaatttacc tccatatttt
     3301 gatacatggg catgagcatt gccgcataaa gtaaaacgat aatcagtgcc acaaagataa
     3361 aaaccagtgg ctgcaccaaa ttcatggtgc ggttgactcg ggtaaaaaag gcttcccaag
     3421 ttttttcagc atagatttcc aactcactac ccagcttgga cttaacttcc ccatactcta
     3481 tgatgagact caattccttc ctaaagaaag gataggttcc tatcgtctga gaaaattcac
     3541 ggccattttt cagggtttga gccagatctt gaccgatttc tttaaagagc tgggaacctt
     3601 gttcctgcat catttgaaaa atctgcgtca gctccattcc ctgtgaaatc atattccccc
     3661 attcacgtgc ataataggct gtcaagtagg tctgcacaaa gattccaata aagggaaggc
     3721 gtgctaagat agaaaagaca ctcatcttag aacttctttt ataaaaagtt agtgctaaaa
     3781 gggcaagcac ggaaacaagc cctaccatgc ctagaaaaat ttgtggcaga ttaccgataa
     3841 ttcgggtggc aatattgcta ctatccagtt gtgggagcag gtaattccgt agccccagca
     3901 taattaagag aagaaaatcc agcaaaatca aaggataggt cgctacttca attaactttt
     3961 tcttgacctt ggccagattg tctagatatt cttctatctt tcccaaactc aggtgaagat
     4021 tgccatgaac ttcagctaga gataactgag tgacaatggt acttgaacat cccaaacttt
     4081 ccatcatttc tgagaatgat ttcccctgag acaagcctgt acgcatctgg gtcacacact
     4141 gcttgtccaa caaggaactc ctatctaaaa aggagatagt ctccaccaga tgaaaaccgc
     4201 tagaaaagag attgttaaat agggtgatga tatttttttg cttagctgta gctaattttt
     4261 tccgtctcag cctgaagact tgtgatatgt ccatctttaa gaagttggtc aatttgctca
     4321 ttccacttgg ctgcttggtg ttcttgataa tctctgtttg caaagtcaac gattcctcct
     4381 cccccgatta atctctggta gcagactcct tgcagaacaa ctgccaattc ttcttcactc
     4441 acacccaact ccagcagacg ctcataaaca cctcggatac tcttggcgtg aatggttgaa
     4501 aagactgtcg cacctgtcaa actagctctg accactgcac gcgccgtctc gctgtcacga
     4561 atttctccga taatcaagag atctggtcga tgacgcaagg aaagtttgat tagattttca
     4621 taggttaggc cgattgcttc gttcaactgc aactgaagca tgtcgtcctg cttgatttcg
     4681 acaggatctt cgatggacat aacttgctgt cctttaaaga gtgacttgga caattcatgc
     4741 atcaaggtcg tcttaccact cccaaccggg ccagcaaaaa gatagagtcc ccgttgcctg
     4801 tactgcttgc ctaattcttc aatatcctga aaccaaaaat gcaagtcctg ctcctcatcg
     4861 tgcaacaaac ggataaccaa actctcatgc ccccgataat cgcctacagt agataaacgt
     4921 agagacgcca tcttctggtc ataggcataa tcacaggaac ccagttgact acgtcttttt
     4981 tctcccacat tcatacccgc cacaaactta aagtgactga taacggctgc aaacttttca
     5041 aaatcatagc taccaatttt acagcgctcg tctcctaccc tcatatgaag ctcataggcg
     5101 tctaacttag ggacaaaata aatatcctgc gtcccttttt tccgagctga acgaatgatt
     5161 tcttgtgcaa tttcttgaac catacttacc tcctcaccta tactattcgc aaagcgttag
     5221 aaaaataaaa aaagcaactc caaaaaagtt acttttctct attttaattt catacggcaa
     5281 gaacgattcc tttcctgact ggtttgtttt tcatagcctg agcgtagttt ttcgtctggg
     5341 ataacctgct catcctgcaa aagagccaaa gaatggtatt gttgtaaata ctcatcacat
     5401 tcatcacacc agcgttggtc ttccgggtcc caaaaaatgg tcatcaagtc gcttctactt
     5461 ttataaagag gggattcttg ttccaatcta aaccagcaag ttttgtagta tttgagagca
     5521 tcataatact ggtcaaattt tctacttgct acaatatctt cttcccaacc ttctaagaac
     5581 caccacggtt caaaatctcc gtacatttct ataacacgat acatattcat tcattccttt
     5641 gtaccgtata ttataaccaa ttccgccaga caaggaaaga aatatgctca tttcttaatt
     5701 ttttgataaa aaatccagcc atctgatgga tgactgaatt tctatttgct tatgtacaaa
     5761 aatattttta ctacttaggc ttctgagata tcgttttcga taatgacctt aatggcatgg
     5821 tggtctgctg ccttactgaa gacttcgtag gctttttcaa tttcactgag tttgaaatag
     5881 tgagttacca atttttccgg ttcaatctta tgactttcaa gtgctttcaa caattgtgga
     5941 gtcgtatttg tagataccaa accagttgtt acattgatgt tgcgaatcca aagtttatct
     6001 aaatcgaatt caactggttt accatgcaca ccacagttgg caaccgttcc gtctacaccg
     6061 ataatctttt gacagaaatc aaatgtttca ggaatcccta cagcttcgat agcgacatcc
     6121 acaccacgac catctgtcaa atcataaatt tctttaatgg cttttttagg gtctgaagaa
     6181 ttaaccttat gagtcgcacc gaatgatagg gcagtttcca agcggttatc gtctaggtct
     6241 accataatca atttagctgg tgaatagaat tgggctgtta aaagagcagc caatccaact
     6301 ggacctgaac caataatggc tacgctgcaa ccaggttcta ctttcccttt taagacacca
     6361 atttcatatc cagtaggcag aatgtctgac agcataacca aagcttcatc tgacaagtct
     6421 tctggagtat ggtaaagagt attatctgca tgagggacac gtagatattc agcctgcata
     6481 ccatcaatca agtgaccgaa aatccagccc ccttcgtctt cacagtgggc ataaattcct
     6541 tttttacagt agtagcattt accacaggca cagacgcaag aaatcaagac cttgtcacct
     6601 tttttgaagt tggaaactcc ttccccaact tcttcaacaa tcccaatccc ttcgtggcca
     6661 agaatggtac cactttggca agtaggaaca tcccctttga taatatggag gtctgttcca
     6721 caaatagtgg tttttacaat acgcacaata gcgtctgttg gcttgcgaat aactggtttg
     6781 tctacatcaa caaaagaagc aagtcctggt ttaacataag tataggcttt cataaagcac
     6841 tcctccgttt ttttatttgt aatatttata gttcaattgt aagccttttt ctttgtttct
     6901 tcaagctttt tttgtgattt ttttaacttt ttatttacca gtaaagtacc ctgtataaaa
     6961 aagcagaagc tagtctctaa cttctgcctt ctctcttatg cttgataacg tttcacacca
     7021 tctaggtagg ttgctaccaa ttccaaatct ttatctaata cgatgaagtc agcgtcgtag
     7081 ccttcacgga tttggccaca gacgtcatcg atgtgaacgg atttagctgg gttgaagctg
     7141 gccatcatga ctgcttcatg cggattcgca attccccatt cgaccacatt cttcaaacca
     7201 tctttgagtt tgaggataga acctgccaag ttacctgtcg atttgaggcg tgcagttcca
     7261 tttgcaacga caactgggaa ttctcccaac atataatctc cgtcttccaa tcccccagct
     7321 gtcatacagt ctgtgataag agcgatgttt tctgttcctt tttgtttgat aagaatttcg
     7381 caagcctttg gatctacgtg gtgaccatca cagatcaact ctgcataggt atgtggcaat
     7441 tggtacatgg ctccaaccat acccaattca cggtgagtca acccacgcat tccattgtag
     7501 gcatgcaccc aaacactcgc tccagcatcg actgcttttt tggcttcatc aaaagtcgcg
     7561 tttgaatgtc caagagcaac cgtcacacct tcgcccgtaa ctgtacgaac aaagtcttcc
     7621 accccatcac gttctggtgc aagggcaatt ttattaagca agccatttgc cgctttttgc
     7681 caagaatgaa actcctcaac acccgggtct ctcatataag ttggattttg tgccccctta
     7741 aaagtttctg tgaaatatgg accttcataa taaatcccac gaatcttagc acctgttgct
     7801 tctttataat ggtttccaag attttcagtg actgcaagca attgctcata agtggctgtt
     7861 aaagttgtgg gtaagaaact ggtaacaccg gtactaagaa gtccttcact catagtatgc
     7921 aatgtacctt caatgttgtt gtccatcaca tctacacctg catatccatg aatatgagta
     7981 tccacaagtc ctggggcaat gctataacct gtatagtcaa tcacctcagc cccttcagga
     8041 atctgctcta catgtttccc aaacttgccg tccacaagtt ccaagtaacc acctcgacga
     8101 actccgtgtg ggtagaaaaa ctgatccgct ttaatatagt taggcataat gttaacctcc
     8161 ttaaaagatt gattctacaa tttattatgt caattacatt ataaaccttt cttttttatt
     8221 tgtaaatggt atagacctat tttttggaga tattttaaaa gagctggatt tctccaactc
     8281 ttctcttttt taatacaagt tattttgatt taaccggctt gtcttgagct gtctgcaaag
     8341 ctgtggcaat cgtatctgca tacaattttg ctcctgcttc gatagtgcta ctctcactcc
     8401 cgaaatgaac ctggtctgtt ccagcccaaa tttctggatg ctctttcgca acttgattcc
     8461 aatctgctat cgtaatgtaa ggtgtcttct ctgccaattc tctcatatag gcagcagcct
     8521 tctcaacgat ggcataggtc tcttttgtct tatctccctc ataaggagtc accaaaatca
     8581 tatggtgtcc cttaggaaga tttttcacga tactgtccca gtcatccttg taattctcag
     8641 gattatttac cccagtcgca atgaccaccg tcttaggtaa aaatttattc tggctattat
     8701 ttagcatgat ttcatttgcg gtcttggttg ttacgctgac ctgcgcgtta atctgtgctc
     8761 caggaagagc tgtctgtagt gctgtatttg cccttaaagc cactgagtca ccaattaaca
     8821 tagtgccatc agcaattccc aaactgtttg catctgcccg ttctgccatc accttggtct
     8881 ggccaatatt tgttgcagct tgcttcaagc cattgacagt caagtctgtc tcaaacgctc
     8941 ccacttgtgg tgccaacaag gtcaccatgc agacaatgaa ggtcaagatt cctgtacctg
     9001 ctgcaagaat tgcgtgaata taaggcaggg gacgaagagt ttgtaaaata ggtgtgttct
     9061 tgcctgcaat ccaaggttcc aatacataaa atgacagact ggcaaagcca taagaacaaa
     9121 tcagagtcag taatacagca agaagatttg atgtcaactg tgagaaaatg atatagaaag
     9181 gccaatggaa aagataaacc gcatagctag tatccgctaa aaagctgata atcttcggct
     9241 cctgtatatg atgtgtcttt tcatgtaaga cacgcgccgc cagaatcatg gcaagagctg
     9301 caagactggc aagcaagaag ccgataagat aggcaaaaag ataagtgaat ttgacaaaga
     9361 aagtcaaaag aactaggaaa ccaaagcctc ctccaaaaac tactaaagtc tttcgtaaat
     9421 cccagatttt atccaactgc ttgacgaggg aagtcgtctg acgaacgcct acaatagttg
     9481 ctaacatact tcccaaaaag aatggataga catgagttaa actggagaaa taaacagagg
     9541 aataagaggt cactagaaaa ctaccaataa acatggagaa gaaactgatc aagaaggcag
     9601 tagcagataa gagaaagatc atccccttca actgaccatt tgatttagct tgtttggata
     9661 agaaccaaac tgccaatccc caaagaatat agtagtgaac ctcaactgcc aaactccaat
     9721 tatgaacaaa caaatgagga atgaactgag attcataact cccacctgtt aagagttcat
     9781 agaagttggt cataaagcct aagacgcccg caatctggcc accaattcca gcaacatagt
     9841 cttggcgaac caagaaagta aaaggcatgg tcaccaagac catcaaaacc acaggtggca
     9901 caatccgata aaagcgtctt ctaaaaaatc ctatcaaatc aatctcattg ttcttagaaa
     9961 attcttcaat gagtagagct gtaatcagga aacctgaaaa tgtgaaaaag acatctaccc
    10021 cgaaaaatcc tccaggaaag atcgtctgaa agaagtgata caagagtacc aaaagtaaac
    10081 ctataatcct aatcaaggaa aaccatttaa tgcgcatacg agtttattct tcctttcatt
    10141 gtacacttta ttctatcaaa aaaagaagaa aaatcctaag agaaaactta ggatttttag
    10201 cttatatcaa ttccatttta gaaattacgt cctgacttat tatagccata tttttccaca
    10261 aaatacttac ggaattccaa gagattgtca tccatgatgg cttgtcgcac ttgcttcatc
    10321 aggttaagca agaagtaaag attgtggtag ctagtcaagc ggataccaaa ggtttcatca
    10381 gccttgagca ggtgacgaag gtaagcgcgt gtatagttat tacatgtgta gcaatcacac
    10441 tcaggatcca gtggcgtaaa gtcctcagca aactgggcat ttttcacaac caaacgtcct
    10501 tgactggtca tacaagtccc gttacgagca attcgagtcg gtaagacaca gtcaaacata
    10561 tccaccccac gaatgacccc atcgatcaag ctatctggcg ctcccacacc catcagataa
    10621 cgaggtttat tttcaggcag cagttgagtt gtaaagtcca agaccgcatt catctcttca
    10681 tgggtttctc ccactgccaa accaccgata gagtagcctg agaaatccat gctgacaaga
    10741 tcgtgagctg attggcggcg aaggtcttca aatcctgcac cttgcacaat tccaaacaaa
    10801 ccttggtcat gtggacgacg gtgagccttc aaaccacact cagcccaacg gctggtacgc
    10861 tcgatcgatt tcttaacgta gtcataaggt tgataaaact gaggacattc atcaaaggac
    10921 atcatgatgt ctgaacccag attattctga atagagatgg ctttttctgg ggataggagc
    10981 atcttagaac catttagatg atttttaaag gttactcctt cttctgtgat attacggcta
    11041 tctgctaaag aataaacctg aaaaccacca ctatctgtca agataggctg gtcccaattc
    11101 atgaacttgt ggagaccacc agcgcgtgca atgagttcat ctccagggcg aagccagaga
    11161 tgataggtgt ttgataggat aattcccgaa cccatctcct tcaattcttc aggtgactga
    11221 gttttgacag tggcttgtgt cccaactggc ataaacatag gtgtcggaaa ggtaccgtgg
    11281 ggagtgatga tttctcctag acgagctcct gtgtgttttt ctttcttaat caaacgatat
    11341 ttgattggtg aatctgacat tttttacctc cgaagctggg aaagacagtc ccagttcata
    11401 cagtgtgccc aaggacacac tgtatgattt tatcaaaaaa agtaggaact gtcacgaact
    11461 atgtataaga actatggtat aataagagaa tgaactatcc aaaaattgat ttaaaaacaa
    11521 ttcgtcagga gtcaaaacat tttcaggctg acaccccccg tctctttctc ctctatatcc
    11581 ttcccagtat gctggtcatc ttatctggtt ttcttaatcc cttatctcgg atccacggga
    11641 ctgttttaga acagccattt ttcagcatac ttggccaaat acttcaaacc tacctctttc
    11701 ctctactagt ctcctttatc gggactattc ttttgaccag ttccgtctat gcaacactga
    11761 cactcatgaa agattctaaa acggagccat ctgtaaaaaa cagcctagct ctctttgatg
    11821 aggagcgctt ttctcaaacc tttttgaccc tacttctcaa acgtttctat ctctttttat
    11881 ggagtatccc taatttactt gggatttacc tcctctttta tagtagcttt ttagccaaaa
    11941 aattcgtcac cctacatcct gagtttccaa atctagatct ctcatcagtt gaaaccgagc
    12001 gtttcctcat ggtctttgga ctctactttc ttgcaagtct catcttgatc attgtaggaa
    12061 atatcctcta tattccacag tattatgcct attcacaggt ggaatttctt ctctgttata
    12121 gtctagacct aggacaggtc ccaccaagac gaatcctaaa aactagccgt tcctttatga
    12181 agggctacaa gttccagcac tttgtcctag atttacagct tcttccttgg tacttcctca
    12241 attggattac ctttggcatt gctagttttt cactcctacc ttatatccaa tgcactaaaa
    12301 ttatgtttta ccgagcagta ttggctcgaa aacgtccaaa agcttgaagg tttttcccct
    12361 caagcttttt tctatcaatg agtttctccg cctaccaact tgagttcctg atctccatgt
    12421 tctatcatag cgccgattgc tgcttctatc cctcgccgaa tatccactaa actcatagtt
    12481 ggagtagtcg gtctgttcac cacctgttcc atcatataag gaatatgcat aaaacctgcc
    12541 ttaacatatg gaaatttctt ttctaccaaa tagagagcct gatacatcaa atggctgcag
    12601 acaaaagtcc ctgccgtatt ggaaacagag gccggtaagc cctctttttt tatagcttga
    12661 accatcgctt taatcggcaa actactaaag taggccgaag caccatctgg gcgaatggga
    12721 cggtcaatcg gttgattatc ttcgttatca gaaatgcgtg catcgtcttg attaatggcc
    12781 actcgttcag gtgtcacact agttcttcca ccagcttgcc caatacaaag gacaaagtca
    12841 ggttgataac gattcatctc ttcttccaat acttgagcag atttgtgaaa aactgtcggc
    12901 acctctagcc aacggacctc agcaccatgg atttcagctg gtaaaccttt aatggcctcc
    12961 aaagttggat tgcccttttc ccctccaaag ggctcaaaac ctgtcactaa tactttcatt
    13021 tctaactcct caaaaccaat tcaatgagac ttttttctta aaaacaagta taacatattt
    13081 cccttattct ggattaaaaa ggattagcga gattaaaatc gaccttcaag tgtatttaaa
    13141 gagtaaaata ctaatcaagg ccaaaatagc tgatccacct tgcttcaaaa taattttttt
    13201 atccgctgtt aaagagccgt aagtcgcagc accaatcaca aataagacaa aaatagtcac
    13261 aatttctaaa ttctgtgaga aataaatgcc ataccagaga aagactccta gcagagcctt
    13321 ataaatccct tgatttttga acaatgaact tactgacgga tgagccaatt cttccttttc
    13381 catattaaat acacgactag tcgcatctga tcgcgtagca atactttcca aataaaaaat
    13441 gtaaaaatgc tccaaagcaa cgatcgttgt taaaataatt gtaataattg acatctattt
    13501 ctccttaaat ttctatattt tcaataccca aaaccaattt atttaataaa cggctgagtt
    13561 ctgttctctc aaactctgtt aagatacttt ccatctcttc cttaaccttg atatgttgca
    13621 gagggggatt caccactaac tgctccttgg catacttcgc agcctctacc aacacttccc
    13681 gctgattttc aggattacga tgacgctcca ccaaaccttc cgtttccaaa atcttgaaat
    13741 gccgtgtcaa agcagcctga tcaattttca aacgctcctg aaccgccatt tggttacaag
    13801 gagaatgctc cagcaaaaac agtaaaatct gataccgtgt caaactaatc ccaagccttt
    13861 tttcaaacaa ttgcgtactc gcttgctcag acaagcggag ttgatacagt aaatcttgaa
    13921 tctctatcat ttcttcctcc tatccattga cttatcaata gttgaccaat caattataat
    13981 ataaagtata gtgaaatgaa ataagagcag gacaaatcga tcaggacagt caaatcgatt
    14041 tctaacaatg ttttagaagc agttgtgtac tattctagtt tcaatctact ataaaataga
    14101 ttgcaagaat gttgcttaga ttattacaag atgataactt tccattttcc attcaaattg
    14161 atactcaatg aaaatcaaaa agcaaactag gaagctagtc gcaggttgct caaagcacag
    14221 ctttgaggtt tcagataaaa ctgacgtggt ttgaagagat tttcgaagag tatgacaaag
    14281 aaaaaacgaa cgagaatcgc ttcccatcac gaaagctaga tctcattcgt caaaatgatg
    14341 ttaccaagtc gggtttatcc attcattgaa acgtgaacgt gatcatagtg attttctgtc
    14401 acactaccac ggtctggcat tgggttccaa gtgttagctg gcccatattt gctatcgaat
    14461 ggagcataga aacgttgttt ccagatgatg taactaatgc cacggctggc catgttttga
    14521 atagcatatt ccgcaatctt atcccctaat tctgaacgtt ctggtaccat aaagtcgata
    14581 gccaaacctt ttccgtgatc tccactgtct cctggacgat aaccgctaaa ggatgtaatg
    14641 ccaaacaagt tagcaatttc ttctttaaag gcagctgttt gtggttgaag acctgcattt
    14701 tcagattttg ctactgcaag tccagcataa tcgggcgcag ctggagcctc gtaagttgtt
    14761 gaaattattt tcgtctcttc tggttgataa gtagaaactg ctgctgtagc tccatttgat
    14821 gatgctactt cttttgcttc tgaacttgtt gtagttgctt ccactgctga tgcagcttga
    14881 gggcttgctt gtgtttcctg cttctcagct ggagtcgttc cctgaggagc ttcttctgca
    14941 actgcactgc ttgtttcggc cgtttgctcc tctgggacag aagtgcccgt agatggtgcc
    15001 acttcttcag aagcaatcac tgtctttgta acttctgaac ttgatgctac ttcttttgga
    15061 gcttctgcaa ttggttgaga aaggtctgca acctgaacag tttgatcatc aacggtcact
    15121 tgattagtgg tcaaatctgc tgtcgcagtt gtcacttctt cactagagtc tgcttgaggt
    15181 gtttggattt caacttctgt tacttcttct gcttcattga cagtcgttgt caaaacagtt
    15241 tctgggaaaa tcaagtccat attagtgatt ttgttcagat tcgcaagcac tgtgacatct
    15301 acacccaagg cttctgcaat ggtgctcaaa gtatcaccat actgtacggt ataacttgtt
    15361 ttgttgtccg ttttagtcaa atcgttttgg atttgctcaa cactacgtgc agtccaaaga
    15421 acttcttctg cttgagttgc caatactggg gcaaatgaca aggctactgt tgacgctaat
    15481 aacattcttt tcttcattct ttcaaattcc tttcaaatga gtacctgtct atcataacac
    15541 aaaaaatgta gaaaaaaagc cctctcgggc ctatttaaca gaactgtcta ttccttgtaa
    15601 caaactagat tacttgaaat agtttgttag gtctctgtca caaaactgac acaatctttt
    15661 tatcacttag tctcaaaaat acgggaaata tctgctaacg cttgaatcct gtgattccct
    15721 tcataagtag actctaaaaa gttgatactt tgaatcccac tattctgggc aaattccaca
    15781 tccagagtcc gatcccctat ataataagta ttatcagaat tcaactgata cttatctagc
    15841 agataggtag ccgcttctgg acttggcttc cgcacaaagc cactctgact ggtcaaaatc
    15901 tctgtaaaat aggattccac ccccaagtcc ttgagaatgg taaaagtgtt gttcccctta
    15961 tgagtatata taaactgctg aattcctgat tcgtctgccc aagctagcac ctcacgcgca
    16021 cctggcatca aaactacctg agcattcttc tcagccagac tctgggcacg cacctgattt
    16081 agcacctcaa catccagatt tctatcttct gccacccgca caagcaaatt ttgcaccgaa
    16141 tacttgaaga taaactctct caccttctcc ttatcataag gaatagaaaa ctgagcaaaa
    16201 gtctcctcaa tccctgataa aatcgcttcg taagagtcca ataaagtccc gtctaaatcc
    16261 caaataaaag ctgttttttg catttcctat cctttcaagc tcatatagcg ctggtaacga
    16321 tagcgtagaa ataaccagcg aaaaccatta tccaagagag accctgccca aataccaggc
    16381 aagccccaac caagcacaat acccatcaga tatcctgtcc caatgcggat acaccacatg
    16441 cctatacttg tcgcataaaa agggaggcgt gcatttccta atccctgcca gactgccgta
    16501 tagatgactg ttcctgtcgt cataggggta ccaagtagtg aaaacagtgt cactagaaca
    16561 ctagcctcca ccgctagaga atcagtcgta tagagatgag ttaatggtac acccaagaca
    16621 tatatactaa aggacagggg caacatgagg aacagagaaa gccaaaaggt ttgtttactc
    16681 aaactagcaa ctcttttcca atcatcctct ccaactgctc gggccaacag catgaccgtt
    16741 gccgtagcga caccaaaggc aggcatatag ttaaactggg tcaagacttc tccgattgca
    16801 ttcccagcaa ctgcctccgt cccaaaagaa acgaccaagg caatgatcac tacatctcca
    16861 gccctcatca taagtcgctc tccagctgct ggtaaagcca aggtcaacag ttccttatct
    16921 aaaccaaaag ttagcttccc ataaggcagt tttaattgtg accacaaaat cacaagacca
    16981 accaaacgag acagaattgt cccccaagca acaccagcta tccccatatc cagaacaaaa
    17041 atagctagac ttgaaaaaag aatattcaag gcattggata aaaaactaac atagagaggc
    17101 agacgtggat tatgcgttgc acgaatcaag gctcccaaac tagtcattaa acctaagaga
    17161 acaatcgatc cgcctaccaa agatagatac agtccaccac tctcagctac atccctctcc
    17221 gtccccaaaa gtcctatcat ctctttccca gcgaagatgg acaaaaatcc taaaaggaaa
    17281 cttaatagta aggtaatctt caacgcctca gtcacatgat aggccaactt cgactggtct
    17341 ttctgcccta tgctttttga aataacactg gaaatagcag ctcccagagc gatgaaaatc
    17401 gcctgataaa tggtgataat attaccagct actgaaaccc ctgaaatagc tatcaatcct
    17461 aaatgagcaa ccaaataact gtccaccatt cccattagca tctgcaaaaa gttttcaccc
    17521 atagctggca atgcaatatt aagaatgtct ttatttttct taaacaatct ctcctcctga
    17581 tgaaaagaaa ctcagttggt ttcccaaccg agtttgctcc ctctgtctta aagtcctaaa
    17641 taagcctcaa ccgctgcttg catgtcagca gctgccactg ttgtcttgtg acgaattgga
    17701 gctatttcaa gcccatcaac tgctggtggc actgcaacgc ctgagatttc atgtaattga
    17761 gccaaggctt caaagtctgt caaacctgct tttccagtta cagcttctac tgcaactact
    17821 gggaacttgt atggactagc tgttgaagca atcactgtct tagttacatc tccagtaacc
    17881 gcttggtatt ttctataaac tgctgaggca accgccgtat gtggatcctc aatataagaa
    17941 tctgactcat aaacacgctt gatttctgcc gctgtttctt cctcagtcgc atattcagct
    18001 gcaaagaggt ccaaaatctc tgcatcaaag tctgtcaact tatattgtcc ttgcgtgttc
    18061 aaggcattca taagttcagc tgtcttttca gcattatttc ccaaaagatg gaaaatcaag
    18121 cgctccaagt ttgaagatac caagatatcc atagatgggc tggttgttac cttaaactca
    18181 cgttttttgt catagacacg tgttttaaag aagtctgtca agacattatt gtcatttgaa
    18241 gcacagatta gcttgccaac tggtaggccg atttgtttgg cataaaaggc agccaagata
    18301 tttccaaagt ttcctgttgg tactgtgaag ttaacctttt caccagctac aatttcacca
    18361 gtcttaacca attgagcgta agcataaaca taataaacaa tttgtggcac cagacgacca
    18421 atgttcatag agttagctga tgaaaattgc aacttgttgg tagtcaattt ttcacgaaga
    18481 gccacgtcgt taaacatgtg cttcacattt gtttgcgcat cgtcaaaatt accatcaata
    18541 gcaataacat gagtattgtc accattctga gtggtcattt gcaactcttg taccttgctg
    18601 acaccaccct ttggataaaa gacgataatc tcagtaccag gcacatccgc aaaccccgcc
    18661 atagcagctt tccccgtgtc accagatgtc gctgtcaaga taacaatctt gttctccaaa
    18721 ccatgtttct tagcagcagt cgtcataaag tatggcaaaa tagacaaggc catatcctta
    18781 aaggcaatcg ttgaaccatg gaaaagttcc aaattgtatt gcccatctaa tttcactaat
    18841 ggtgcaatag ctggagtatc aaatttgcta tcgtaggcat tgttgataca gtagtccaac
    18901 tcctcagctg taaagtcatc taaaaatgct gacaaaacta gcttagcaac ttcctggtaa
    18961 gaagcatctt tcaatttgtc aaagtccaaa tctacctttg gataagtaac cggtgtaaac
    19021 aaaccgccgt ccgtcgccaa accttgcaaa attgcttggc tggcagttac tgtattgttg
    19081 gcatcacgcg ttgattgata aactaatgtc attactctct atcctttatc tatagtctcg
    19141 tccattatat catgattttt cagaaaattc tccctttata agagaaatta taggctcaat
    19201 tcttctctca aaggctgtaa ataggtcatt tcttccttat ttctcaccac tagtccctcc
    19261 tcagctagta ggagtaagtc tttggaaaat tcaataatcg tagtttcttc ctcatctgta
    19321 agatttttct tagaaaattg tctccttaaa gacttgtaat cataaccaaa tactttaaag
    19381 aaaggtgctg tttctaagta agcttctaac ttgtctaaat taaccaataa tcccaagtga
    19441 aaagctgcag aagcaaaagt cctatcaagt ggctgtgtac acacactacg aaactcaact
    19501 gttcctcgag tcgttaaatc ttggtactgg taactacgat gagtttcaaa atccttctct
    19561 tgggggtaaa taataacctc atccccattc agagcaaatg cttggatttc ggacgtagcc
    19621 aaatagtccc cagcctgaat aggataaaaa taataggtct gcccatcacg ttccgcagta
    19681 aaaatcgcag aatgatttag atagtcaaaa aaatcagctt catcattaag gagtctagca
    19741 ttgaccccaa cattctctgg atagatacca tgcatagatt cttcccagaa aatatccctt
    19801 gaaattttcg tatcccaatc cgcacccgaa aattcagagt ttgcaaataa ataagcctta
    19861 gccgcttcaa tttgagtaaa agcattaatc acccgtaagt agttggtttt tgaaatatcc
    19921 agctgaacct ggctcccaca gataaaagta ccatattcag ggaaatgatg taaatctgat
    19981 ttaataatat ttctactcaa attcaaataa tccatcaaca tctgatagcg tggataagcc
    20041 actggacaat tctcattttt atcccagttg ggatggatac cacagccaac aatagcatga
    20101 tttgattcag ctaactttct ctgaattaca ttcatataat tattgaaacg attttcgacc
    20161 tcttgaatcg tttcagcctt accaaatgca aactcaatcg ttgtatagga aacttcaaat
    20221 aaaatagcat cctgacttat cggatctact aactggatca gattgccaaa atcatctacc
    20281 tttgctacgg tgagatccaa agtagaaact aaataatgaa atagatcctt tataacttca
    20341 acatctgtag catccccttc taaacttgca acaggatact ccaactcaat tccgacaaac
    20401 aattcaggat tttctttaat atttttcaaa taccgatgtt ttaataaatc gatagaacaa
    20461 gacatactac cctacacttt cttaatctaa ataaaatttg aataactata attataccaa
    20521 aaataatata aaaaaggaac gaagaataac aacgttccta acactatact ataccggcgg
    20581 ccggggtcga accggcacgt ccttgcggac actggatttt gagtccagcg cgtctgccaa
    20641 ttccgccacg ccggcaaata gtaactgggg tagctggatt cgaaccaacg catgagggag
    20701 tcaaagtccc ttgccttacc gcttggctat accccaataa tataaaatag gcgagtgatg
    20761 gggatcgaac ccacgcatgc cagagccaca atctggtgtg ttaaccactt caccacaccc
    20821 gccataattc tattaacacg ggcagtagga attgaaccca cactgaaggt tttggagacc
    20881 ttagttctac ctttaaacta tgcccgtaaa atggaagggg agggattcga acccccgaac
    20941 ccgaaggagc ggatttacag tccgccgcgt ttagcctctt cgctaccctt ccaaaatata
//
LOCUS       DAVBCV010000037        20241 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBCV010000037 DAVBCV010000000
VERSION     DAVBCV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20241
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.037"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            21..866
                     /locus_tag="VQ516_001348"
     CDS             21..866
                     /locus_tag="VQ516_001348"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2511780.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VQ516_001349"
     CDS             complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VQ516_001349"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2511781.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            2422..4746
                     /gene="pflB"
                     /locus_tag="VQ516_001350"
     CDS             2422..4746
                     /gene="pflB"
                     /locus_tag="VQ516_001350"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2511782.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(5113..5331)
                     /locus_tag="VQ516_001351"
     CDS             complement(5113..5331)
                     /locus_tag="VQ516_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511783.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(5427..5665)
                     /locus_tag="VQ516_001352"
                     /pseudo
     CDS             complement(5427..5665)
                     /locus_tag="VQ516_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5658..5942)
                     /locus_tag="VQ516_001353"
     CDS             complement(5658..5942)
                     /locus_tag="VQ516_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511784.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            complement(6554..6724)
                     /locus_tag="VQ516_001354"
     CDS             complement(6554..6724)
                     /locus_tag="VQ516_001354"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2511785.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            complement(7374..8597)
                     /locus_tag="VQ516_001355"
     CDS             complement(7374..8597)
                     /locus_tag="VQ516_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2511786.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            8765..10087
                     /locus_tag="VQ516_001356"
     CDS             8765..10087
                     /locus_tag="VQ516_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2511787.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            10089..11975
                     /locus_tag="VQ516_001357"
     CDS             10089..11975
                     /locus_tag="VQ516_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2511788.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            12143..12487
                     /locus_tag="VQ516_001358"
     CDS             12143..12487
                     /locus_tag="VQ516_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2511789.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            12497..13909
                     /gene="lacG"
                     /locus_tag="VQ516_001359"
     CDS             12497..13909
                     /gene="lacG"
                     /locus_tag="VQ516_001359"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2511790.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDITLAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            13979..15658
                     /locus_tag="VQ516_001360"
     CDS             13979..15658
                     /locus_tag="VQ516_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2511791.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMIAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLDIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(15784..17223)
                     /locus_tag="VQ516_001361"
     CDS             complement(15784..17223)
                     /locus_tag="VQ516_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2511792.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(17227..18576)
                     /gene="trkA"
                     /locus_tag="VQ516_001362"
     CDS             complement(17227..18576)
                     /gene="trkA"
                     /locus_tag="VQ516_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2511793.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            18764..19612
                     /locus_tag="VQ516_001363"
     CDS             18764..19612
                     /locus_tag="VQ516_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2511794.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            19627..20175
                     /locus_tag="VQ516_001364"
     CDS             19627..20175
                     /locus_tag="VQ516_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2511795.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
BASE COUNT         6133 a         3435 c         4141 g         6532 t
ORIGIN      
        1 tataatatag ggagtaatct atgtatctta ttgaaatttt aaaatctatc ttcttcggga
       61 ttgttgaagg aattacggaa tggttgccga tttccagtac aggtcacttg attttagcag
      121 aggaattcat ccaataccaa aatcaaaatg aagcctttat gtccatgttt aatgtcgtga
      181 ttcagcttgg tgctatttta gcagttatgg tgatttattt taacaagctc aatcctttta
      241 aaccgactaa ggacaaacag gaagttcgta agacttggag actatggttg aaggtcttga
      301 ttgctacttt acctttactt ggtgtcttta aatttgatga ttggtttgat acccacttcc
      361 ataacatggt ttcagttgct ctcatgttga ttatctacgg ggttgccttc atctatttgg
      421 aaaagcgcaa taaagcgcgt gctatcgagc caagtgtaac agagttggac aagcttcctt
      481 atacgaccgc tttctatatc ggactcttcc aagttcttgc tcttttacca gggactagcc
      541 gttcaggtgc aacgattgtc ggtggtttgt taaatggaac cagtcgttca gttgtgacag
      601 aatttacctt ctatcttggg attcctgtta tgtttggagc tagtgcctta aagattttca
      661 aatttgtgaa agccggagaa ctcttgagct ttgggcaatt gtttttgctc ttggtcgcga
      721 tgggagtagc ttttgcggtc agcatggtgg ctattcgctt cttgaccagc tatgtgaaaa
      781 aacacgactt cacccttttt ggtaaatacc gtatcgtgct tggtagtgtt ttgctacttt
      841 acagttttgt ccgtttattt gtataagaaa aaccttgaag gggcaactct tcaaggtttt
      901 atactcttcg aaaatctctt caaaccgcgt cagctttatc tgcaacctca aaacagtgtt
      961 ttgagcagcc tgcggctagc ctcctagttt gctctttgat tttcattgag ctttaaaatc
     1021 cagtcatggt aatccccaat aggcggacac ctctttcttt cttgcttaat tcttcataga
     1081 gttgcagggc tatttggctt atctgactag catcttgtgt tttttgagca agactttttc
     1141 gtttggtaag agttgaaaag tcctcgtagc ggattttcaa aatgacaatt tttccagctt
     1201 tttcttgttg atgtagattg agagcgactt tttctgatag aagagtcagc tcttttttga
     1261 tatcttcctc agcaaggaga atcttcccgt aggttttctc cttgccgatt gatttacgga
     1321 tgcgattgga tttgactgga gagttgtgaa tgccacgagc ctttcgatac agatcatagc
     1381 ctagtctacc aaaacggtct attagggtta cctcaggaac ttcaagtaaa tcagcaccag
     1441 taaaaacgcc catttgatga agacgttcta ctgtcttttt tcctactcca tgaaatttgg
     1501 aaatatccat ttgtttgaga aaatcctcag cctgttcagg tagaatcact gtcaaaccat
     1561 gtggtttttg ataatcactc gccattttag ctaagaattt gttgtaagaa acgcctgcgg
     1621 aagcagttag atggagttct tgccagatat ctttttgaat gaggcgagca attttgaccg
     1681 ctgacttgat accgagttta ttttctgtca catccaaata ggcttcgtca atgctcatgg
     1741 gttcaatcaa atctgtatag cgcttaaaaa tagctcgaat ctggagtccc acagacttgt
     1801 atttctcata attccctgag ataaagacag cctggggaca acgttcataa gcttccttgg
     1861 aactcatggc agaatggaca ccaaaagctc ttgcctcata actacaggta gaaacgactc
     1921 cccgtccacc tgtttgccga gggtcgcttc caataatgac aggttttcct ctgagtttag
     1981 gattatccct gatttccact gcagcaaaaa aggcatccat gtcaatatgg atgatttttc
     2041 ttgacaaatc atttaacaaa ggaaaaatca acatgcctag caccttttta tactcttcga
     2101 aaatctcttc aaaccacgtc agctctatct gcaacctcaa aacagtgttt tgagcaatct
     2161 gcggctagct tcctagtttg cttttcgatt tccattgagt gttactgctt attttctttt
     2221 attataccct tttttctgaa aaaaagaaaa aaggacttta ttttttcaaa aatataatac
     2281 agtttgaaat aaaatataga ctgttttaga aaagaaagtg taaaaatagg gaattttcac
     2341 ttgttgaaat cggttactat atggtatact tgtcttatga atgtaacaga tgactgttac
     2401 tagaaaaaag aggacattaa tatggttgtt aagacagttg ttgaagcaca agatattttt
     2461 gacaaagctt gggaaggctt caaaggcgta gattggaaag aaaaagcaag tgtatcacga
     2521 tttgtacaag ctaactacac accttatgat ggagacgaaa gcttccttgc aggaccaaca
     2581 gagcgttcac ttcacatcaa gaaaattgta gaagaaacta aagcacacta cgaagaaact
     2641 cgtttcccaa tggacactcg tccaacatct atcgctgata tccctgctgg atttatcgac
     2701 aaagaaaatg aagttatctt cggtatccaa aacgatgaac tcttcaaatt gaacttcatg
     2761 ccaaaaggtg gtatccgtat ggctgaaact actttgaaag aaaatggata cgaaccagac
     2821 ccagctgttc acgaaatctt cactaaatat gtaacaacag ttaacgacgg tattttccgt
     2881 gcctacactt caaatattcg tcgcgctcgt cacgcacaca ctgtaactgg tcttccagat
     2941 gcatactcac gcggacgtat catcggtgtt tacgcacgtc ttgctcttta cggtgcagac
     3001 tacttgatgc aagaaaaagt aaatgactgg aatgcaatca aagaaatcga tgaagaaaca
     3061 atccgtcttc gtgaagaagt aaaccttcaa taccaagcat tgcaacaagt tgttcgcctg
     3121 ggtgaccttt acggggttga tgttcgcaaa ccagcgatga acgtgaaaga agcaatccaa
     3181 tgggttaaca ttgctttcat ggctgtctgc cgtgtgatta acggtgctgc tacatctcta
     3241 ggtcgtgtac caatcgtatt ggacatcttt gcagaacgtg accttgctcg tggtacattt
     3301 actgaatcag aaatccaaga attcgttgat gatttcgtta tgaaacttcg tacagttaaa
     3361 tttgctcgta caaaagctta tgaccaattg tactcaggtg acccaacctt tatcacaact
     3421 tctatggctg gtatgggtaa cgacggtcgt caccgtgtta ctaagatgga ctaccgtttc
     3481 ttgaacactc ttgacaacat cggtaactca ccagaaccaa acttgacagt tctttggact
     3541 gacaaattgc catacaactt ccgtcgctac tgtatgcaca tgagccacaa acactcttct
     3601 atccaatacg aaggtgtaac aacaatggct aaagacggat atggtgaaat gagctgtatc
     3661 tcatgctgtg tgtctccact tgatccagaa aatgaagaac aacgccacaa catccagtac
     3721 ttcggtgctc gtgtaaacgt tcttaaagcc cttcttactg gtttgaatgg tggttacgac
     3781 gatgttcaca aagactacaa agtatttgat atcgaaccaa tccgtgacga agttcttgaa
     3841 tttgaatcag ttaaagcgaa ctttgaaaaa tctcttgact ggttgactga cacttacgta
     3901 gatgccttga acatcatcca ctacatgact gataggtaca actacgaagc tgttcaaatg
     3961 gccttcttgc caactaaaca acgtgccaac atgggattcg gtatctgtgg atttgctaac
     4021 actgttgata cattgtcagc tatcaaatac gctacagtta aaccaatccg tgacgaagat
     4081 ggctacatct acgattacga aacaatcggt gactacccac gctggggtga agatgaccca
     4141 cgttcaaacg aattggcaga atggttgatc gaagcttaca caactcgtct acgtagccac
     4201 aaactataca aagacgcaga agctacagta tcacttttga caatcacatc taacgttgct
     4261 tactctaaac aaactggtaa ctcaccagtt cacaaaggtg tatacctcaa cgaagatggt
     4321 tctgtgaact tgtctaaact tgaattcttc tcaccaggtg ctaacccatc taacaaagct
     4381 aaaggtggtt ggttgcaaaa cttgaactca ctttctagcc ttgactttag ttatgcagct
     4441 gacggtatct cattgactac acaagtatca cctcgcgctc ttggtaagac tcgtgatgaa
     4501 caagttgata acttggtaac aatccttgat ggttacttcg aaaacggtgg acaacacgtt
     4561 aacttgaacg ttatggactt gaacgatgtt tacgaaaaaa tcatgtcagg cgaagacgtt
     4621 atcgtacgta tctctggata ctgtgtaaac actaaatacc tcactccaga acaaaaaact
     4681 gaattgacac aacgtgtctt ccacgaagtt ctttcaatgg atgacgcctt ggatgcattg
     4741 agctaatcaa gttcttgaat aataaaaagg aaccctcggt caaacgactg agggttttgt
     4801 gcttgggata gtatgagcaa ttccttcggc gcaatatgca atgtttttgg gaaggaatag
     4861 tggaccgaat ctgaaatata atagattgaa actagaatag tacaccttta cttctaaaac
     4921 attcttagaa atcgatttga ctgtcctgat cgatttgtcc tattcttatt tcattttact
     4981 atagtccgct gaaacggctc aaacagtgct agaaattgac tggaattctc taagtgattt
     5041 gttccgattt tatttcattt catcataaaa ataagatgac tggctaattg acaatcatct
     5101 tgtttgatac tgttaataaa attcgcgaag tttatcttga ttttctttaa aaaatttttc
     5161 taaccaatcc ataccataag cataactaaa accttctaat agaacagaga ggtcaatagt
     5221 atcaaatccc atgtctttta aatatttaaa ggtatcgaat ggtgaatgaa agtttttgta
     5281 tttaataatt gcaccaatat cttgcaaatc ctgctctctg atactcatca tcttcattcc
     5341 taacacatac tcaataggga ctactaaaac ggtcagattc tcaaatgagt acaaggattc
     5401 gcataaactt aagggaggtt gtttgttcat atttgctttg caacatgatt gttgagccat
     5461 aactcttcgt ggatgttaag attaaactgt tttcctacac gagcaataat ttcattgatt
     5521 ttctgatttt ggtcataaaa agcatcaaca tcttgtgtgg cacgtaaacc atgatattct
     5581 aagacataac cataattgtt aaagtcagat tttcctgaat taatttctta tttagggctt
     5641 ggaagaccgg tttcatttta tccatgtttg tatttctcca tcaatttttg aacattggtt
     5701 aacttagcag gtttatttaa tgaaatcaca tatttatgag ttggagtatg ctcttgaaag
     5761 gcatacttca agctaatttt ttgaaaacgg gttagttctt tggagtctag caacttttct
     5821 acatcttggt ttgttaaata agaatagtct tcaaaaataa cttttcgcaa atagcgaaac
     5881 caatggcttg cgttgaattg tgattgtttg gatgcctgta tcatataatc gtaccaatcc
     5941 attgtcttcc tcctataaaa tttagtttat attattgtag tttatattca aaaatgtagt
     6001 ttttcttaat tagctcgtat ctataggatg atactgaaaa tcttcttgtt gctttaaatc
     6061 acaatcgaaa aattctgttt ttcatcttag aaaagaatgt ttagaaatgt aaaatgaacc
     6121 ttttttctta tccgaaaacc ttactaaagt atataattta ggcagtcaat tctgtatgat
     6181 aatgtttgta ctatcgtcga gtttttgctt gattgcctaa cgagataaat tttagaagag
     6241 ttttcggtgt gatttgacaa gtttctgact ggaaggatag ttacactagt attgtttatt
     6301 aaaatgagca gatagagaaa atttggaatc tatttgctta ttatcaatcg tttttttagc
     6361 ttatttgtct tagattggta tagaactgag ctatggatta atcttcttct atcttatcct
     6421 gttcctaaat atgttggtag aaaaatgaag cagttgttac gagtgtctgt attgggaaaa
     6481 attttgtcta tagaattctt taaatgatat gaaggctagg aatgccaaaa cactgacata
     6541 tttcgtttaa agtctatcca gtctcttcaa ttgtatttgt agaaatgcta tcagtaatat
     6601 attcgcgaac actatgattt acaacagtgt ggtcgtacat ctttgtaact ttattataca
     6661 acaaccattt acaggtgtaa agttgagcag tgtctttagt aaatttaggg acttgttttg
     6721 tcatagtttt gatacctgta ttcgttgttt catgagcatt tgacgtttct tttcatgtag
     6781 gcatttcaac aattagaatg atatttttcc gaggatgaac ttatatattg tgacttttta
     6841 gagttatgat gcctcttgat ttgactaccg ctatcaattt catccacttt tacagtttca
     6901 tggttttatg aaccgaaaca tctaggtaca ggttttagca tcaattgatt gtttttgagc
     6961 catatgaagt tgaaacgtgg agatgctttt cttgtgataa cgccctattc aaagaggaat
     7021 aaccgttttt ggagagagtt cagtactatg tggcgctaaa tactatatgt aaaacatata
     7081 ttaaacaaga tttaactaag gagaattgta aaaacaaaaa tgagaatttc atataaagtt
     7141 tattctagga gtatgtatct tgaataaaaa tcgttattta agttcagggt tatgatgaat
     7201 gtttttataa taataaaatg cagctagaag aaaagttatt ttaatataaa tcactcaaca
     7261 tagagagaag gcggtttcct atatggaagt cgtcttgttt tcatattgtc taaatacgag
     7321 atatctacta caggttatag ttgatttaaa caggtctata gatttctaat atattaaggt
     7381 gaaataatat cttgtaatac attgctatgt tctatgaaaa aagcgacgat agctaatgca
     7441 caagctccta tacttccacg atgtttgttg taaggtaaaa tttcaatttc tatattacga
     7501 gtaaagggaa cgaagtggag ctggtcttgg attttatccc tgacttcatg agtgaaaggt
     7561 tgataattaa gcaattgact gttgatatag attttatcag caggattgat gaggaggaga
     7621 tttgcaatag aagtggctaa taaattgact cctttatcaa tttgttgacg taaagcggag
     7681 tcgcctaaat tataagccgt taaaagggtg tctaaatgaa tgtctttttc agtctttaca
     7741 aggcttttta gtacagttag ttgggaattt ttaaataata attgggcgtg tttgattaac
     7801 caagcatccg aaatatatgt ttgtaaacaa cctttttttc cgcattcaca atattgccca
     7861 ttcaattcga caatggtgtg tccaatttct ccgatataag gatttttaga ggctcctatt
     7921 ttttcttttg tgaaaaagga agtgtaaata cctaatccag cgtgtaggaa aataaagtta
     7981 tcgggggtat tgtgtggatt aaaaaggtat tgtcctatag ccatacaatc gatattattt
     8041 tttacaataa aaggaaaatt gaatctttta attacattta ataaattaaa agattcccat
     8101 atggggttgt ttgtaatgat acttccggag tctttgttat aatgtccagg tattgataag
     8161 ccaaatccaa gtaattttgt ttccaaaggg acgtactcta tagctgtttt tatcatattg
     8221 gtaatggtgg aattgatttc tgaaatagga taatcttcat tcaaaatttc tgtctcgaaa
     8281 agagttttcc cttgtaagtc acatagactg agaaagatac ctaggacgtt tatttcaata
     8341 cctccaacaa catacggaat attatctgtg atagaaatag tttttctacg tcttccagat
     8401 ccggaaaact cgctaaattc atcaccagtt tcgtatatga gggactgttc gcttagctct
     8461 tttatgatgt tagtcgttgt tgctggagta agtcctgttt cattaacaat atctgtacgg
     8521 gatgttgctg gatttcgata tagaaactcc agtatagtgg ctctattttg taaaaaggct
     8581 gattgttttg taatcatttg tttcctcctt tggtttattt tatattaaac ataagaaaaa
     8641 atcaattaaa attattattt tatacaaaga aaaaaatatt gtaaaaacaa caaaaaatat
     8701 attgacaacg ctttctgtta gtggtatatt taatttataa aaaataaaaa aaggagttcc
     8761 ggtgatgaac aatattttag cgtttttaga aacaaaagtc gctccgtttg gtgaaaaagt
     8821 tggcaaccaa cggcatttga aagctattcg tgaaggattt atgatggcaa tgcctttgat
     8881 tttagtcgga tctttatttc ttattctaat cagttggcct caagaggatt ttacaaattg
     8941 gctgaatagt gttggattgc taagtatctt gacaactatg aatcagtcaa cagtagcgat
     9001 tatctccttg gtcgcttgtt tcggtattgc ctacaggttg tcggaaggat atggtacaga
     9061 tggtccgtcg gcagggatca tagccttatc cagttttgta ttgatggcac ctcgtttttc
     9121 gagtatggtt tatgataaaa atggggagca ggtcaagcag ttatttggcg gcgcaatacc
     9181 attttctagc ctgaatgcat cttctttgtt tatggcgatt actattggat tggttacagc
     9241 agagatttat cgtatgttta tccagcgcgg aattacgata aaaatgccaa gtggtgtccc
     9301 agatgtggta agtaaatcat tttcagctct tttacctggc tttactactt ttgttttgtg
     9361 ggctctggtc ttaaaaggtc ttgaagcggc aggagttgca ggaggtctca acggactcct
     9421 aggtgcaatt gttggaacac cgcttaagtt aattgcagga acgcttccag gtatgattct
     9481 atgtgttatt gtaaactcat tcttttggtt ctgtggagtt aatgggggac aagttttaaa
     9541 tgcttttgta gatccagttt ggttacaatt tactacagaa aaccaagaag ctgtggctgc
     9601 aggacaaaca ctccaacaca ttattacatt accgtttaaa gatttatttg tatttattgg
     9661 tggcggtgga gcgactattg gtcttgcgat ttgtctcttc ctatttagta agagtcgtgc
     9721 gaataaaaca ttaggtaagc tagctattat accgtctatt tttaatatca atacagctat
     9781 tctatttacg tttccaacag ttttaaatcc gattatgctg attccgttta ttgctactcc
     9841 tacaatcaat gccttaatta cctatgtatc aatggctgta ggattagtac cctacacaac
     9901 aggtgtaatc cttccgtgga caatgccacc gatcatagga ggcttccttg cgacaggagc
     9961 tagttggcga ggagctctat tacaagttgt tttgattttg gtttctgtag caatttatta
    10021 tccattcttc aaaattgcag ataaacgcaa tcttgaaaaa gaaaaagcta ctgttggagg
    10081 gaaataagat ggttatcaga gtatttgatc agcagaaaaa tacttattct agctttgcct
    10141 tagaggaatt aagttactat ataaatcggg tctttaagac tgacatagaa cttgtcgagg
    10201 agaaggaagc ggatattttt gtaggattag tcaataaaga ggatagaaaa gaccatgttc
    10261 ttatctcatt agacaagggt aaggggagaa ttgagtctaa tacaattgta ggtttgctta
    10321 ttggaattta ccgaatgttt catgaatttg gggttgtgta tactagacca gggcgcggac
    10381 atgactttgt tccagagtta cgatttgaag attttttaga taaagagcta tctatagatg
    10441 aaacagccag ttactatcat aggggagtat gtatagaggg agcagattca tttgaaaata
    10501 tactagattt cattgattgg ctacctaaga ttgggatgaa cagttttttc attcagtttg
    10561 aaaatcccta ttcttttttg aaacgttggt atgaacatga gtttaatcca tacctaaata
    10621 aagaaaaatt ctcaaatgaa ttagtacaag aattgagtga taggttggat aaagaattgc
    10681 aaaaaagagg tcttattcat catcgtgttg gtcatggatg gacaggtgag gttttaggtt
    10741 actcttcaaa atttggctgg gaatcaggtc ttagtatttc agaggagaag aaaccctatg
    10801 tcgctgaaat aaacgggaaa cgagaattat ttaatacggc tccgatttta accagtctgg
    10861 atttttcaaa tccagatgtg gcggataaga tggtagaaat tatcaaggat tatgccaaga
    10921 aaagacctga tgttaactac ttacatgtat ggttgtcgga tgctcgtaat aatatttgtg
    10981 aatgcgaaaa ctgtagacaa gaattggttt cggatcagta tattcgtatt ctcaatcaat
    11041 tggatagggc tttaacgagt gagggattag atacaaagat ttgttttctg ctttaccatg
    11101 agttgttatg ggcgcctcag aaagaaaaat tagataatcc tgaacgcttt accatgatgt
    11161 ttgcaccgat tacaagaaca tttgaaatga gttatgcaga tgtagatttt gacaattcca
    11221 tacctacgcc taaaccttat ctgcgtaata aaattatact tccgaattct cttgaggaaa
    11281 atttatctta tctttttgag tggcaaaaaa catttaaagg agatagtttc gtatatgact
    11341 atcctttagg gcgtgctcat tatggcgatt taggctatat gaaaattagt caaaccattt
    11401 acagagatgt atcttatctt tctaacctac atttgaacgg ttacatttcg tgtcaagaat
    11461 tacgtgccgg attccctcat aattttccta attatgttat gggggaaatg ctctggaaga
    11521 agacaagaag ttatgaagaa ttgattgaag aatacttttc tgctttgtat gggtcaaatt
    11581 ggcagtctgt tgttgaatat ttagaaaaat tatccagtta ttcctcatgt gattatttta
    11641 atgcaattgg cagtcgtcaa aatgatgttt tagcgaatca ttattatata gcttacaatc
    11701 tagctgataa ttttttgcca attattgagg aaaatatttc taagttatta aatagtcaaa
    11761 aggatgaatg gaaacagctc agttatcatc gtgaatatgt tgttaagatg gcgaaggctt
    11821 tatatcttca agcaaccgga aaaacaaggc aagctcaaga tgaatggaga aatgtgttga
    11881 attatatccg tgggcacgaa ttgctatttc aatctaattt ggatgtttat cgtgtaattg
    11941 aagtagcaaa aaattacgct ggtttccact tataaatcat aagtatagaa aatgaaccaa
    12001 ggtattcaga gaagattgat cctaaatatt atgaaattta aggattttta agatatttag
    12061 ggtcgacttt ctatttatat cgtagcgaag tcattttaat aatgatgtgt aaaagatgga
    12121 tcaagattga ggaggaagaa agatgaaatc aaaagaagaa ataaatatgc ttggttttac
    12181 aattgtcgct tacgcaggag atgcaaggtc agatttgatg gatgctttgg cgtttgcgag
    12241 agatggatat tttgaacagg caagagaatt ggttgagtct gcaaacgact caatagtgtc
    12301 tgcccatcga gaacagacta atttattagc ggaggaggca tctggagata attttgaagt
    12361 gagttttatc atgattcatg gtcaagatac cttgatgaca acgatgctat tgtatgatca
    12421 ggtcaagttt tttattgatg aatatgaacg aattcgaaag attgaagaac atattggttt
    12481 gcaatgagga ttagtcatgg aaaatttaca ggttaaagcc ttaccgaagg agtttttatt
    12541 aggagctgct accgctgctt atcaagtaga gggtgcaact agggtagatg gcaaaggaac
    12601 aaatatgtgg gatgtttatt tgcaagaaaa cagtccgttc ttaccagatc cagctagtga
    12661 tttttattat cgttacgaag aggatataac tttggcggca gaacatggtt tgcaggcttt
    12721 gcgtttatct atttcttggg ttcgtatatt tcctgatata gatggggagc ctaatatatt
    12781 agctgttcgt tattaccata gagtctttca gtcttgctta aaacataatg tgattccgtt
    12841 tgtttcttta catcattttg attcgcctca gaaaatgtta gaaacagggg attggttgaa
    12901 tagagagaat attgatcgtt tcatacgata tgctcgcttt tgtttccaag aatttacaga
    12961 agtcaagcat tggtttacaa tcaatgaact gatgtctctt gctgcaggtc aatatatagg
    13021 aggtcagttt cctccaaatc atcattttca attatctgaa gcaattcaag cgaatcataa
    13081 tatgttgttg gcgcatgctc ttgcagtcct cgaatttcat caattaggaa ttgagggaga
    13141 gataggttgt attcatgctt taaagccagg ctatcctatt gatgggcaaa aagaaaatat
    13201 tttggcagct aaacggtatg atgtttataa taataaattt ctattagatg gaactttttt
    13261 gggctactac agtgaggaca cgctttttca cttgaatcaa atattggaag ctaataattc
    13321 tagctttatt attgaagatg gtgatttaga aattatgaag agagctgcag ctcttaatac
    13381 gatgtttggg atgaattatt atcgttcaga atttattcgt gaatacaaag gtgaaaatag
    13441 acaagaattt aattcaacag gaataaaagg acagtcttct tttaaattaa atgctctagg
    13501 tgaatttgta aaaaaacctg gtattccgac aacagattgg gattggaata tttatcctca
    13561 agggttattt gatatgttgc ttcgtatcaa agaagaatat cctcaacatc cggtcattta
    13621 tttaactgaa aatggtacag cccttaaaga agttaagcca gagggcgaga atgatattat
    13681 tgatgacagt aagagaatcc gttatattga gcaacattta cacaaagttt tagaggctcg
    13741 agatagagga gtcaatattc aaggctattt tatatggtct ttgcaagatc aattttcttg
    13801 ggcgaatggc tacaataagc gatatggtct tttctttgtt gattatgaaa cacagaagag
    13861 atatattaag aaaagtgctc tttgggtaaa agggctaaaa cggaattaag gttagcgatt
    13921 tgactgatgt ttaatatatt ttaactatga gcttaaattt tttacaggag gagtttttat
    13981 ggataagtta gtcgctgcca ttgaaaagca acaagggaaa tttgaaaaaa tttctactaa
    14041 taattatatg atagctatta aagatggatt tattgctact atgcctttaa ttatgttttc
    14101 aagctttttg atgattatta ttatgattcc taaaaatttc ggagtagagt taccgagtcc
    14161 agctattgcc tggatgagga aagtgtatat gctaaccatg ggagttttgg gtattattgt
    14221 ttcagggaca gttgcaaagt cattggctgg aaatgttaat agaaaaatgc ctcctggaaa
    14281 ggtaataaat gatacttctg caatgttggc ggccatatgt ggctatcttg tattaactgt
    14341 aacgcttgta gttgatgaga aaacgggatc tacaagtttg tcgacaaact atttaggatc
    14401 tcaaggattg ataacttcgt ttgtcagtgc ctttattact gtaaatgttt accgattctg
    14461 tattaagcga gacattacta ttcatttacc taaggaagtt cctggggcta tatcacaagc
    14521 ttttagagat atttttcctt tttcttttgt tttacttatt agtggtttgt tagatattgt
    14581 atctcggttt agtttagatg ttccttttgc ccaagtattt caacaactat tgactcctat
    14641 ttttaagggg gcagaatcat atcctgctat gatgttgatt tggtttatgt gtgctttgct
    14701 ttggtttgtt ggaattcatg gaccatctat tgtcttacct gctgttacag ctttacaact
    14761 gagcaatatg gaagagaatg ctcaacttct tgcaaatggg cagttccctt atcattcttt
    14821 aacacctaat ttcgggaatt atatcgctgc tattggagga acgggggcta cctttgttgt
    14881 accatttatt ttgattttct ttatgcggtc taaacaatta aaatcggtag gtaaagctac
    14941 aattactcct gttttatttg cggtaaatga acctcttcta tttggtatgc ctgttatttt
    15001 gaatccctat ctttttgtcc cttttttgat gactccacca gtgaatgtat ttctaggaaa
    15061 ggtatttatt gatttctttg gaatgaatgg attttatatc cagttacctt gggcctttcc
    15121 tggtcccttg ggattgttaa ttggaacgaa ttttcaactt atctcctttg tatttttatc
    15181 tttgatgtta gttgtcgaca tattgattta tttgccattc tgtagagcgt atgatagaca
    15241 gttactggtg aaagaagata ttgcaagctc aaatgatatt attttagagg aggatacaag
    15301 tgaaataatg cctggtgaga tagatgtaat aaaaagtaag gagttgaaag tactggttct
    15361 ttgtgcaggg tctggaacaa gtgcgcaatt agccaatgca attaacgagg gcgctcaatt
    15421 agcagaagtt agagtgattg cgaattcagg agcgtacgga gctcattatg atattatggg
    15481 tgtttatgat ttaattattc tggccccaca agttcggagt tattatagag agatgaaggt
    15541 ggatgcagaa agattagata ttcagatagt tgctaccaga ggaatggaat atattcattt
    15601 aacaaagagt ccaagtaaag ccttacaatt tgtattggag cattaccaag ctgtgtagta
    15661 agtttttcca tcttttattt gagtaaagat tttgcttaca gataggcttg gatttaaaaa
    15721 cgttccccct tttttaatat aagaatccct ctttcacaat tgtaaaaaga gggattttgt
    15781 attttatctc ttagaccaag ttctcttcat aaagagaagg aggattgggt aaatctccaa
    15841 gcgccctgca atcattgcaa aggataggag aatttttgag atgggactaa agattgagaa
    15901 actagaagtg gttcctagaa taggcccgat attattgaaa cagctaaaga cagcgctggt
    15961 cacgaccaga aaatcattgc tatctaggct gacaataaag ataagcgcta gcaaaatcat
    16021 agcatagatg acaaagtact tgagaatctt atgctgggta tctttgtcaa tcaccgtttt
    16081 attaacatgg agggtcaaaa cacggtgggg cgataggatt gacaaaattt ggtttttggc
    16141 aatttttgaa aggatgaggc ctcgaataat cttgagtcca cctgcagttg atccagcaga
    16201 gccaccgatt gccatgagga aaaggaggat aaactgggag aagaggggcc agttggtaat
    16261 atctccatat ccaaaaccag ttgttgtaat gatgttggaa acctggaaga aggtcatttc
    16321 aaagctcttt gaaaaccctg ggtagaggta gagggtgttg aggctaatca agcctgtaga
    16381 aaccagtaca atgaccaagt aagccctaag ctcttcatct ccaaagaagg ccttgatgcg
    16441 acggagcatg aggtagtagt agaggttgaa atttactcca aaaaccagaa ctccgatact
    16501 gaccagatag gtaatcagtg agctgccata gtgggcaatt ccgtcgttat agacggtaaa
    16561 gcctccagtt cccgctgtcc ccatagcaat gacaaaacta tcgtagagag gcataccagc
    16621 tagataatag atgatgacaa agagggagaa gagagctaga taaaggagat agagaatctg
    16681 ggcagtgttt tttagtttgg atacaacctt gccaaaaaca ggacctggaa cctcagcctt
    16741 catcacctct aggtggctat ttttggcatt gtccataata gcaagtgcaa aaacaagcac
    16801 tcccatccct ccaatcaagt gggtaaaact tcgccagaag aggagggaac gactgagaac
    16861 tgaaacgtcg ttcaaaatac ttgctccagt agtcgtaaag ccggaactaa tctcaaaaaa
    16921 ggcatcaata aggctgggga tttgcccaga aaagacaaag gggagaccac caaagaaaga
    16981 ccaaaggatc caacagaggg caacgatcaa gactccctcc ttggcataaa tccgttgatt
    17041 ttttggcttc tgtaaactcc ctgaaccgcc taacaatacg agaatcccta tggtcgaaaa
    17101 gagggctgta aagacttggc tcgattcacg gtaatagaca gcgatagcca caggaaccaa
    17161 gaggagaaca gcttcaatca aaagtaactt tgaaaggagg taacgaatca tacttttatt
    17221 catttcttac ctcgcaatca agtcataaat cttggtgatg tttggcaaca aggttgttac
    17281 taggagcttg tctccaactt ccaacatatc ctccccagtt gggaaaatag tcttgccctt
    17341 tcgaataatg gctgcaataa gaaccccttt tttcaatttc agttgagaaa gaggtttggc
    17401 agtcatttta ttggcttcct tgatatggaa ttgcagggtt tcgatttggc cattggctag
    17461 atggtgcatg gcttgaaggt ctgaatactg ggcattaact cgaccacgaa taaagtgcat
    17521 aatcgtatct acagcgatgc ttttaggtgt gatgatactt gaaaaatcag gcgcattgat
    17581 aatctcgagg agactggtac gattgacctt agtaatattt ttctgtacac ctaccctgtc
    17641 aaggaacata gatgtaatca gattttcctc atcgactcct gttagagtcg caacggcatc
    17701 atagtgttga gcactttctt ccagcaggat atcttttgcg gttccatctc cttgaacgat
    17761 gtagagattt gggaatttct cgctaaagaa gctggcgatt tcaggattga tttcaatgac
    17821 ttttgtatcg atacgactat ctttgagaat accaagtaga taataggcaa ttctacctgc
    17881 cccaacgatg agaaggctct tcacggcgcg tgatttaaaa taattatgga agagtatcat
    17941 atcgacacgg ttaccagtga caaagattct atctttatcc tgtacagtca tgtcaccgct
    18001 tggaatgata atttgatgat ccctctctat cgcacagaca atgacattac caaatttttt
    18061 acgaaaatca gaaatgggca tttggcaaag accgctggtg gacttgacga caaattccat
    18121 gaggctaacg cgtccaccag caaagcgttc gacagacagg gcgttgggga agtcaatgat
    18181 attcgcgata gcacgggcag ccaagagctc aggattaacg ataagagaaa aaccgagaat
    18241 attcttttcc ttgaaataag agttagaata ttcagggttc cgcacccgaa cgatagtttc
    18301 tttagctccc attttcttgg ctagaactgc tgcaatcatg ttgacttcat cgtgctcagt
    18361 cagggcgata aagatatcac aatcttggac gctggcttgc tcaagaatgg caaaatcggc
    18421 cccgttacca aggataccaa tgatatcaaa gcgactgaca atatgattga gaacagcttc
    18481 gtcttgctca atcagcaaaa catcatgctt ttctgcaacc aaggagcgac agagggcaaa
    18541 accaactttt ccccctccga caaggataat tttcataata aaacctactt tttcatgatg
    18601 taactatcat accctttttc aagaaaaaat gcacctacta gctaataaca agagctttta
    18661 gtgaaaattc gctataagat aaaactatac cctaaccaat tgaaatagct attagcgact
    18721 ttctctgaaa tatggtatga taaaggatat acaaggagat aaaatgaata ataatttact
    18781 ggtattacaa tcagactttg gtctggttga tggtgcggta tcggctatga ttggagtggc
    18841 tttagaagag tctccaacct taaaaatcca tcacttgacg cacgatatca cgccttataa
    18901 tatttttgag gggagctatc gtctctttca gacggtggat tactggcctg agggaacgac
    18961 gtttgtatcg gttgtcgatc caggtgtcgg ttcgaaacgt aagagtgtag ttgccaagac
    19021 tgcaaaaaat caatacattg tcacgccaga taatgggacg ctttccttta tcaagaaaca
    19081 cgttggcatt gtagccattc gtgagatttc tgaggtggcc aataggcgtc aaaacacaga
    19141 gcattcttat accttccacg gtcgtgatgt ctatgcctat actggtgcta aactggccag
    19201 tggtcacatt acttttgagg aagtagggcc agagctcagt gtggaacaga ttgtagagct
    19261 tccagtcgta gcgaccatca tagaagatca tctggtgaag ggagccattg atattctgga
    19321 tgtgcgtttc ggttcgcttt ggacctctat cacacgggaa gaattttaca agttggaacc
    19381 agaatttggt gatcgttttg aagtgaccat ctatcatgct gatatgctgg tctatcaaaa
    19441 tcaggttgtc tatggcaaat catttgcaga tgtgagaatt gggcaaccca tcctttacat
    19501 caactctctc tatcgtttag gtttggctat caaccaaggt tcctttgcca aggcctataa
    19561 tgtaggtgtc ggttcatctt ggaccattga aataaagaaa atagaaggat aaaataggag
    19621 aatagtatgg aaatcaaatt tacaattaaa caagttgttg ctgtcggaat tggcgctgcc
    19681 ctctttgtcg tcatcgggat gatcaacatt ccaacccctg ttccaaatac aagcatccag
    19741 cttcagtatg cggtacaagc gctactttct attatttttg gaccgattat cggtttgctt
    19801 gttgggttga ttggtcatgc aatcaaggac tctcttgttg gttatggtct atggtggact
    19861 tggattatcg ctagcggact ctttggttta gttgtgggac tttttagaaa gtacgttcgc
    19921 gtgatcaatg gtgtttttga ctggaaagat attcttattt ttaacctcat tcaactactt
    19981 gcaaatgccc ttgtttgggg tgtcttggca ccacttggag atgttgtgat ttatcaagaa
    20041 gcggcagaaa aagtatttgc acaaggaatt gttgcgggaa ttgccaatgg tgtatctgta
    20101 gctattgcag gaactcttct cttacttgcc tatgcaggaa cccaaactcg tgcaggaagt
    20161 ttgaaaaagg actaatactc aatgaaaatc aaagagcaaa ctaggaagtt agccgcaggt
    20221 tgctcaaaac agtgttttga a
//
LOCUS       DAVBCV010000038        20158 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBCV010000038 DAVBCV010000000
VERSION     DAVBCV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.038"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            63..527
                     /locus_tag="VQ516_001365"
     CDS             63..527
                     /locus_tag="VQ516_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2511796.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            685..1110
                     /gene="lacA"
                     /locus_tag="VQ516_001366"
     CDS             685..1110
                     /gene="lacA"
                     /locus_tag="VQ516_001366"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2511797.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1141..1656
                     /gene="lacB"
                     /locus_tag="VQ516_001367"
     CDS             1141..1656
                     /gene="lacB"
                     /locus_tag="VQ516_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2511798.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1667..2596
                     /locus_tag="VQ516_001368"
     CDS             1667..2596
                     /locus_tag="VQ516_001368"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2511799.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2598..3578
                     /gene="lacD"
                     /locus_tag="VQ516_001369"
     CDS             2598..3578
                     /gene="lacD"
                     /locus_tag="VQ516_001369"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2511800.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3812..4648
                     /locus_tag="VQ516_001370"
     CDS             3812..4648
                     /locus_tag="VQ516_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2511801.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALIQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4684..5001
                     /locus_tag="VQ516_001371"
     CDS             4684..5001
                     /locus_tag="VQ516_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2511802.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5003..6697
                     /locus_tag="VQ516_001372"
     CDS             5003..6697
                     /locus_tag="VQ516_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2511803.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERSGKSNDELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6906..8312
                     /gene="lacG"
                     /locus_tag="VQ516_001373"
     CDS             6906..8312
                     /gene="lacG"
                     /locus_tag="VQ516_001373"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2511804.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPSDVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8355..8537
                     /locus_tag="VQ516_001374"
     CDS             8355..8537
                     /locus_tag="VQ516_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2511805.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQQS"
     gene            complement(8815..9576)
                     /locus_tag="VQ516_001375"
     CDS             complement(8815..9576)
                     /locus_tag="VQ516_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2511806.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ516_001376"
     CDS             complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ516_001376"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2511807.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ516_001377"
     CDS             complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ516_001377"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2511808.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ516_001378"
     CDS             complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ516_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2511809.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13717..13980
                     /locus_tag="VQ516_001379"
     CDS             13717..13980
                     /locus_tag="VQ516_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2511810.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ516_001380"
     CDS             13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ516_001380"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2511811.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            15974..18424
                     /gene="phtA"
                     /locus_tag="VQ516_001381"
     CDS             15974..18424
                     /gene="phtA"
                     /locus_tag="VQ516_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2511812.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            18582..>20158
                     /locus_tag="VQ516_001382"
     CDS             18582..>20158
                     /locus_tag="VQ516_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2511813.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKL
                     VDDILAFLAPIRHPERLG"
BASE COUNT         6438 a         3612 c         4158 g         5950 t
ORIGIN      
        1 aactagaatt ctagttttgc aggaagtcta ataaagaaac tagcaaggtc aggccttact
       61 ttattggtgg acatccagga tttaattgtc ttctacttga tgatgaagtc tatgaagatt
      121 attatctaga gtttgagaaa gaagagattt gctctgttcc acgtcctttc ccagaaacag
      181 gtatgttgga tttccaagat agaagtccat ggctagaggg acaaaaagaa atagatctta
      241 gttatgatct tttcagcaca gatgcagtga ctttggatga attgcaatct cgaacaattg
      301 cccttcgttc tcttaaacat gataagggat tgaaagtgca ttttgcagaa tttccaaacc
      361 tcatcatctg gtcaactttg aacaagggac ctttcattac ctttgaacca tggtctggct
      421 tgtcaacatt ccttgaagaa ggagatcatt tagaagataa gaagaatgtt tgtctcttag
      481 aagccaatca ggttgaagaa ttagggtttg agatagaagt tttataaaaa aagtaaaaaa
      541 taaataacaa aaacaaacac aaactgttga cttcttcaaa caatagtagt atattatgtt
      601 tgtgaggact aattgactgt tttttaaaga cagccaatac actgctggaa tccagcatag
      661 aaaaaataaa aaggagtata caatatgtct attgttatcg gtgcagatgc tgcaggtttg
      721 agattgaaag aagttgtgaa agacttctta gaaaaagaaa acttccacct tgtggatgtt
      781 acagctgaag gtcaagactt tgttgatgtg actctcgctg ttgctgcaga agtaaacaaa
      841 gaagaacaaa accttggtat cgtgattgat gcttatggag ctggtccatt tatggttgca
      901 actaagatca aaggaatggt tgctgcagaa gtatctgacg aacgttcagc ttatatgact
      961 cgtggccaca acaactcacg tatgatcact atgggggcac aacttgttgg tgatgaattg
     1021 gctaaaaaca tcgctaaagg atttgttaat ggtaaatacg acggtggacg tcatcaaatc
     1081 cgggttgaca tgttgaacaa aatgggctaa ttagattaca gtaagaaagg taagttaaaa
     1141 atgagaattg caattggatg cgaccacatc gtaacagatg aaaaaatggc ggtttcagaa
     1201 tttttgaaat caaaaggata tgaagtcatt gactttggta catatgacca tacacggact
     1261 cactacccaa tctttggtaa aaaagtaggg gaagctgtaa ctagcggcca agctgatctt
     1321 ggggtatgta tctgtggtac tggtgttggt atcaacaacg ctgtaaataa agttccaggt
     1381 gttcgttctg ccttggttcg tgatatgaca acagcccttt atgctaaaga acaattgaac
     1441 gctaacgtta tcggctttgg tggtaagatt actggtgaat tgcttatgtg tgatatcatc
     1501 gaagctttca ttcatgctga atacaaacca tctgaagaaa acaagaaatt gattgcgaaa
     1561 attgaacacc ttgaaagtca caatgctcaa caaacagacg caaacttctt tacagaattc
     1621 cttgaaaaat gggatcgcgg agaataccac gactaagagg tgacctatga ttttaacagt
     1681 cacaatgaac ccatccattg atatttccta tcccttggat gagttgaaga ttgatactgt
     1741 caatcgtgtg gtggatgtaa ccaaaacggc tggtggtaag ggacttaatg ttacccgagt
     1801 actttcagaa tttggtgatt ctgttcttgc tactggttta gtgggtggca aacttggtga
     1861 gtttttggtt gaacatatcg atgatcaagt aaagaaagat ttcttttcta ttcagggaga
     1921 aactcgtaac tgtatcgcta ttctccacgg tgacaaccaa acagaagttc ttgaaaaagg
     1981 tcctgaagta ttggaacagg aaggtcaaga ctttttggaa catttcaaaa aactcttgga
     2041 gtcagttgaa gtagtagcca tctcaggtag tctgccagct ggccttccag ttgattacta
     2101 tgcgagcttg gtagagcttg ctaatcaagc tggcaagcta gtagtcttgg actgctctgg
     2161 tgcagcactt caggcggttc ttgaatcacc ccataaaccg acagtaatca aacccaataa
     2221 tgaggaattg tctcagcttc taggaagaga agtttctgag gatttggagg aattaaaaga
     2281 agtccttcaa gaatctttgt ttacagggat tgaatggatt atcgtttcac ttggtgccaa
     2341 cggtactttt gccaaacatg gtgacacttt ctacaaggta gatattccta gaattcaggt
     2401 ggtaaatcct gttggatctg gagactctac tgtggcagga atttcttcag gacttcttca
     2461 caaagaatcg gatgcaggat tactcatcaa ggcaaatgtc cttggtatgc tcaatgctca
     2521 agaaaaaatg actggtcatg tcaacatggc caactatcaa gctctatatg atcaattaat
     2581 agtaaaagag gtataaaatg gctttaacag aacaaaaacg tgtacgctta gaaaaacttt
     2641 ctgatgaaaa tggtatcatc tcagctcttg catttgacca acgtggtgct ttgaaacgcc
     2701 tcatggctca acaccaaaca gaagaaccaa ctgtggccca aatggaagaa cttaaaatct
     2761 tggtagcaga tgaattgact aaatacgctt catctatgct tcttgaccct gagtatggac
     2821 ttccagcaac taaggctctt gatgaaaaag ctggtcttct ccttgcttat gaaaaaacag
     2881 gttatgacac aacaagcaca aaacgcttgc cagactgctt ggatgtttgg tctgcaaaac
     2941 gtattaaaga agaaggtgca gatgcagtta aattcttgct ttactatgat gtagatagct
     3001 cagacgaact caatcaagaa aaacaagcct acatcgaacg catcggttct gagtgtgtgg
     3061 ctgaagatat cccattcttc cttgaaatcc ttgcttacga tgaaaaaatt gcggacgcag
     3121 gttctgtaga atacgctaaa gtaaaaccac acaaagttat cggtgctatg aaagtctttt
     3181 cagacccacg ttttaacatt gatgtcttga aagttgaagt tcctgttaac attaaatatg
     3241 ttgaaggctt cgctgagggt gaagtggttt acacacgtga agaagcagca gccttcttca
     3301 aagcgcaaga tgaagcaacg aacttgccat acatctactt gagtgctggt gtatcagcta
     3361 aactcttcca agatactctt gtatttgctc atgaatcagg tgcaaacttt aacggagttc
     3421 tttgtggtcg tgctacatgg gcagggtcag ttgaagctta catcaaagat ggtgaagcag
     3481 cagctcgcga atggcttcgc acaactggat ttgaaaacat tgatgagctc aataaagttc
     3541 ttcaaacaac agcgacttca tggaaagaac gtgtgtaaga aagtcctcct agtttaggaa
     3601 catgaatcta aaaaaattta aaaaaagttg tatgtaaagg tttacaaaat aacttacttg
     3661 tgctatactt aaatcacaag ttaatacaag gtgagtgtta ctaagtaata ttaggcatga
     3721 tcacaggtga attagaaatc agctgatttt ctagttcatt tgtggtcatt ttttgtactc
     3781 atataccttt aagatataaa aggaggttga catgtatcga attctaaatc caatgaatca
     3841 caatgtctcg cttgtcagaa atgataaggg agaagaggtg attgtaattg gtaaggggat
     3901 tgcattcgga aagaagaagg gggatttgat tgctgaaaat catgttgaga aaatctttcg
     3961 gatgaagacc gaagagtcca gagaaaactt tatggctctt ctcaaagatg ttccgcttga
     4021 ttttatcaca gtgacctatg aaatcattga taagctatca aagaaatatc attatccgat
     4081 tcaagagtat ctctatgtaa ccttgacaga tcatatttac tgttcttatc aagctctaat
     4141 tcaaggaagg tacaaggata gtaatctgcc agatatttct gctaagtatc ctgtcgcttt
     4201 tcaaatcgca aatgaagctt ttgaaattta ccgtcagaag ctagcagatc attttcctga
     4261 ggacgaaatt attcggattg cttatcattt cattaatgct gaaggtgaaa atgaagtgga
     4321 acttgtggag tcgattgata agaggaaaga aattctcagg aatgttgaag aagttttaac
     4381 ggactatgca attcaacgaa ctaaaaagaa taaccatttc tatgatcgct ttatgatcca
     4441 tttgaattat ttcttggatt atttagacag atctagagat gataaccaat cacttctgga
     4501 tatggaagat catattaaac aatcctatcc aaaagccttc gagattggtt ccaagatcta
     4561 tgatgtgatt acgcaacata cgggtcttga tttgtataaa agtgaacgag tttatctagt
     4621 tctacatatc caacgtttat tgtcataaaa atttatttaa aactatataa ggagaattct
     4681 atcatgaata gagaagaagt aacattgtta ggttttgaaa tcgtagccta tgctggcgat
     4741 gctcgttcaa aactattgga agccttgaag gctgctgaag ctggtgattt tgaaaaagcg
     4801 gacgctctgg tagaggaagc tggtagctgt attgcagagg ctcaccacgc gcaaacaagt
     4861 ctattgacta aggaagcttc tggtgaggac ttagcgtata gtgtgaccat gatgcatggt
     4921 caagatcact tgatgacaac tatcttgtta aaagacctaa tgcaccattt aattgaactt
     4981 tacaagagag gagttaaata aaatggataa gttaataatt tttattgaga aaggaaagcc
     5041 tttctttgag aaattatcga gaaatatata cttaagagcg ataaaagatg gttttatttc
     5101 cagtatgcca gcagtattat tctcaagtat ttttatacta atagctgctg tgcccaatat
     5161 ttttgggttt aaatggtctg atgaacagtt agcttttatt ttgaaacctt acaactactc
     5221 aatggggatt ttagctctct tagtagctgg gacaactgca aaatcgttaa cagattcagt
     5281 taatacacgt agtatggaga aaaccaacca aatcaactac atgtcaactt ttttagcagc
     5341 tgttgttgga ttgttaatac tggcagctga tccaattgaa ggtggatttg ctaatggtct
     5401 tttaggtact agaggattac ttactgcatt tttggcagct tttattacag taaatatcta
     5461 taaagtatgt attaaaaata atgtaaccat tagacttcct gaagaggttc caccaaatat
     5521 tgcacaagtt tttaaagatg ttattccgtt tgccttatct gttctttcaa tatatggatt
     5581 ggatttaatt gttagaaata tttttggtac aaatgtagca gaatctgttg gtaaaatatt
     5641 agcccctcta ttttcagcta ctgatggtta tatcggttta gctattgtat ttggtgctta
     5701 tgctttcttt tggtttgttg gaattcatgg gccatctgtt gtagaacctt taattgtagc
     5761 tatcagttat gcaaatatag aagctaatgt tcagcttgtg caagctggta tgcacgcaga
     5821 taaaatttta aatccagtta ctcaaacttt tgttgtaaca atgggtggta ctggtgcaac
     5881 actagtagtt ccatttatgt ttatgtggtt atgtaaatca aaaagaaata gaattgtcgg
     5941 acgtgcgtct gtagttccta ctttctttgg cgtaaatgaa ccaatcctat ttggtgctcc
     6001 aattgtttta aatcctatct tctttattcc gtttgttact gctcctatta tcaatgtttg
     6061 gattatgaaa ttttttgtag atgttctaca aatgaatagt ttcagtatta ttttaccttg
     6121 gacaacacct gctccaattg gtattgttat gggaacggcg ctggctccat tgtcattcgt
     6181 cttggctata actttaatca ttatcgacac tttaatctat tatccatttg ttaaagttta
     6241 tgaccatcaa attttagaag aagaacgttc aggtaagtct aatgatgaat tgaaagaaaa
     6301 agttgctgca aacttcaaca ctgcaaaagc ggatgctatt cttgaaaaag cgggtgtcga
     6361 tgcagcacaa aataccatca ctgaagaaac aaatgtcctc gttctctgtg caggtggagg
     6421 aacaagtggt ctccttgcaa atgctttgaa taaggcagca gcagaataca atgtccctgt
     6481 gaaagcagca gcaggcggct atggtgctca ccgtgaaatg ttaccagagt ttgatcttgt
     6541 tatccttgcc cctcaagttg cttcaaactt tgaagatatg aaagcagaaa cagataagct
     6601 cggtattaaa ctagcgaaaa cagaaggcgc tcaatacatc aaattaactc gtgatggaaa
     6661 aggtgctctt gcattcgtac aagcgcaatt cgattaaggc tagagactct gaaatagtct
     6721 cctatcgtta cggaaatcgc tatggcgaat ttcctattat taattcgtcg gtaaaaagat
     6781 atcgttttta cctcctcatg tcacaattcg gtgacttggt acaagaagtg agatggagaa
     6841 ggatggctca ctgactcctc tcctctcact tttactttat ttaaatcaag aaataggtga
     6901 aaaaaatgac aaaaacactt ccaaaagact ttatttttgg tggcgcaaca gctgcctatc
     6961 aagcagaagg tgctacacat actgatggta aaggaccagt agcgtgggat aaatatcttg
     7021 aagataacta ctggtacact gcagaaccag ccagtgattt ttacaatcga tatccagttg
     7081 acctcaagct agcagaagag tatggtgtca atggtattcg aatttctatt gcttggtcac
     7141 gtattttccc gactggttac ggccaagtaa atgctaaagg tgttgagttt tatcataatt
     7201 tatttgcaga gtgtcacaaa cgtcatgttg agccctttgt aactcttcat cactttgaca
     7261 cgccagaagc tctccactca aatggagact tcttaaaccg tgaaaatatc gaacattttg
     7321 tagactacgc tgccttctgt tttgaagaat ttccagaagt aaactattgg acaaccttta
     7381 atgaaattgg gccaattggt gatggtcaat acttggttgg gaaattccct ccaggtattc
     7441 agtacgacct tgccaaagtc ttccaatcgc accacaatat gatggtgtct catgcgcgtg
     7501 cagttaagct atataaagat aaaggctata aaggcgaaat tggtgttgtt cacgctctgc
     7561 caactaagta tcctttagat cctaaaaatc cgtcagatgt tcgtgcagct gagttggaag
     7621 atattattca caataaattt atcttggatg caacttatct aggtcgctat tcagctgaaa
     7681 cgatggaagg tgtcaaccat atcttatcag tcaatggtgg tagcttggat cttcgtgaag
     7741 aagattttac agcattagaa gcagcaaaag acttgaatga tttccttgga atcaactact
     7801 atatgagtga ctggatggaa gcctttgatg gagaaactga aattatccat aatggtaagg
     7861 gtgagaaagg aagttctaaa taccaaatca aaggtgttgg tcgtcgtgta gctcctgatt
     7921 atgtaccacg tacggattgg gattggatta tctaccctca aggtttatat gaccaaatta
     7981 tgcgtgtgaa gaaagattat cctaactaca agaagattta catcactgaa aatggtctcg
     8041 gctataaaga tgagttcgtc gataacactg tttatgatga tggacgtatt gattatgtga
     8101 aacaacactt ggaggtcttg tctgatgcga ttgcagatgg agcgaatgta aaaggttact
     8161 tcatttggtc attgatggac gtcttttcat ggtcaaatgg ttatgaaaaa cgttacggtc
     8221 tcttctatgt agactttgaa actcaagaac gttatccgaa gaaatcagct cactggtaca
     8281 agaaagtagc ggaaactcag attatagact agtagaatta gtcattagat atagaatttt
     8341 agtgagtcaa aaagatgttc aaagatttta tccaatctat ttatgaaaaa gtttatatta
     8401 taaatttcga aaaatgctct caaataccgt gtttgacgag tgaagaattg aaaagtctat
     8461 ttttaaattt tatcaatcct gaagaatggg atactatctc ctttgattca gattttatgc
     8521 catttcaaca atcgtaacca atttctcaaa aaagttaaat cttatattta gtactctgca
     8581 aaactcttat ctaatcacgt tgcttatact caatgaaaat caaagagcaa ctttaaacta
     8641 ggaagcgagt cgcagatttc tcaatgcata gctttgagga attgggcaaa aagtctttga
     8701 tatagaaaaa gcatagtatc aggtgtttca acacctgata ctatgctttt tattgtggga
     8761 agatttactt tttttcttct gaaattgagt tgttacccag gctctttcag tttattaagg
     8821 cttgatgact ttaatgtgtt tagatagctt aaaaaggatt gaatcactta gtttagaatc
     8881 tgaaacaata gtatcaagat ttgatacatt ataaaaagta taaaaatcaa acttattgaa
     8941 cttactatga tctgcgagta aatatttttt attagaatta tttaaagcga tgcgttgagc
     9001 ctctccctct tcctcgctaa aagtagctag agctccgttt tgaataccat tacagctaac
     9061 gaaagcttta gaaaattgga gattagagag attttgtagg gtcaatgtgc caacaaaagc
     9121 acctgtaata tcgcgataat ttccacctat caaaatcaaa tctgttaatt ttcgttcgct
     9181 taaaatcaga aaaacaggta gactgttggt tacgacgcgg atattgtcaa taggcaactc
     9241 acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa atagtttctc tttcttctac
     9301 tagactgcct gcaaaatggg ctatttcttg tttttctgcc gtttggaggg cttgtttttc
     9361 aatatttgat cgctcattag tcaaaaggga gttggttcga agtttttcag ctccaccatg
     9421 cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa tagcgccttg cagtcatatc
     9481 tgaaacggct atttcgtcca taatctgttt aactgttatg gttcctttac tatttactat
     9541 ctctaaaatt ttggctaatt tttcttgttt gagcatatta tcacctcgtt tcctactact
     9601 atcttaccat aaacaaactc atcattcaaa tacaaaaaca acaaaatgaa acaaaaacaa
     9661 aaatatcgaa gtttgttttc aaaactttcg atatttttgt tgggttataa ctttgatgtt
     9721 tctagtttac tttttgatga ttgagagtga tggagaatta gtctaaaccg tagttatagt
     9781 catcgtcttg catggcttca acttcgccaa gaaggtaacc atttccgact tgagagaaga
     9841 agtcatggtt ggaagttcct gttgaaatac cgttcataac gattgggttg acatcttcag
     9901 ctgaatctgg gaaaagtgga tcttgtccca tgttcatgag agctttattg gcattgtagc
     9961 gaaggaaggt tttaacctct tcagtccaac caacaccgtc ataaagactc tctgtgtagc
    10021 cttcttcatt ttcataaaga gtatagagta ggtcgtacat ccattctttg agtttttctt
    10081 gctcttcttc aggcaattca ttgaaaccaa gttggaattt gtaaccaatg taggttccgt
    10141 gaacagactc gtcacgaata atcaatttaa tgatttctgc aacgttggct agtttgttgt
    10201 taccgagata gtagagggga gtgaagaaac cagagtagaa gaggaaggtt tcgaggaaga
    10261 cgctggcaac tttcttttca agtgggctgc cgtttaggta gatttcgttg acaatctcag
    10321 ccttcttttg taggtaagga ttggtattgg tccattcgaa aatttcttca atctcagcct
    10381 tagtattcaa ggtagaaaag attgatgagt aagatttagc gtggacagat tccataaatt
    10441 ggatgttatt gaaaacagct tcctcatgtg gtgtacggat gtctgcgcga agggcttgaa
    10501 ccccagtttc agattgcata gtgtcaagaa gggttaaacc accaaaaact tttccgacca
    10561 agtctttctc tttgttagat agctttctcc agtcatccaa gtcgtttgat aagggaatac
    10621 gtgtatcgag ccaaaattgc tccgtcagtt tttcccaagt tgatttgtcg atgacatctt
    10681 cgatggcatt ccagttaatg gctttgtagt aagtttccat ttaaaatctc tttctgtgtt
    10741 tagtattgcg aactcacaat tatttctact ttaccataat tctataggag tatcgcacaa
    10801 aaagtcggaa gcccgacttt taaaatgtta cataaattat gttatgacat agtagatttg
    10861 attttatcag tgctgcttag ggaaaaataa tgtttctatg ctagaaacta aatcacacag
    10921 ctttcacatt ggttggcgcc gacttctcca ccgtcatctg taaaggtacg gacgtagtag
    10981 atagacttga ttcccttgtt aaaggcatag ttacgaagga tggacaagtc acgtgtcgtt
    11041 tgtttatttt ccctcttcca ttcgtaaagg ccttttggaa tgtcactacg catgaagagg
    11101 gtgagtgaaa gtccttgatc cacgtgttca gtcgcagcag cgtaaacatc gatgacttta
    11161 cgcatatcca tatcgtaggc agaagtgtag taaggaatgg tttctgtaga caagccagca
    11221 gcagggtagt agattttacc aattttcttc tcttggcgtt cttcgatacg ttgcgtaatc
    11281 gggtggatag aagcagaaac gtcgttgata tagctgatag aaccatttgg cgctacagca
    11341 aggcgatttt ggtggtaaag accatcttct tgaaccttgt cgcgaagttc agcccaatca
    11401 gcaacaccag ggataaagac atttttgaag agttctttaa cacggtctga tgttggaaca
    11461 aattcaccag ttacatactt gtcaaagtaa cttccgttag catagtctga tttttcaaag
    11521 ttgtggaagg taataccacg ttcacgcgca atattgtttg actctaccaa ggtccagtag
    11581 ttcataagca taaagtagat gcttgtaaat tcaacagact caggtgaacc atattcaatg
    11641 agttgttggg caaggtagct gtgcagtccc atggcaccga gaccaaaggt gtgggcttgg
    11701 ctatttccat ggtcaatcgt tggtacagct acgatatgtg aactatctgt aacgaaagta
    11761 agggcacgaa ccatagcacg gatagaacga ccaaaatcag gtgaagtcat catgttaacc
    11821 acgttggttg aacccaggtt acatgaaaca tctgttccca tttgaaggaa ttcttgagca
    11881 tcgttgatca agcttggttc ttgaacttga agaatctcag aacacaagtt actcatgata
    11941 atctttccat caacaggatt tgcacggtta gccgtatcga tgttgactac ataaggatag
    12001 ccagactctt gttgcaattt agagatttca gtttccaaat cccgcgcctt gatttttgtc
    12061 ttgcgaatat ttggatttgc gaccaattca tcgtattttt cagtaatgtc gatgtaattg
    12121 aatggcacac cgtattcttt ttctacagag taagggctga agaggtacat ttcttcattt
    12181 ttacgagcca attcgtagaa tttatcaggt actacaacac caagtgatag agtcttgaca
    12241 cgtacttttt catcagcgtt ttctttctta gttgaaagga aagcgatgat atctgggtga
    12301 aagacgttga ggtagacaac accggcacct tgacgttgcc ccaattggtt ggagtaagag
    12361 aagctgtctt caaaaagctt cataacagga acgacacctg aagcagctcc ttcatagcct
    12421 ttgataggtg caccagcttc acgaaggttg ctgagggtaa ttcccacacc accaccaata
    12481 cgtgaaagtt gaagagctga gttgatagaa cgtccgatag agttcatatc atccgtcact
    12541 tggattagga aacaagatac caactctcca cggcgagcac gtccagcatt caagaaggaa
    12601 ggagtagcag gttggtagcg ttggtggatg atttcattgg caatatcgat tgcaacagct
    12661 tcattcccat cagcgaaata aagggcgttg aagaagacac ggtcttccat attttcaaga
    12721 tagtattcac cgtcattagt ctttaaggca tattgattgt aaaatttata agctgccatg
    12781 aatgacttga attggaagtt ttggtctttg ataaattgag ctaattcttc caagaactct
    12841 ggacggtatt tcttgataaa ggctgtttcg atgtagttgt gttcaatgag gtaattgatt
    12901 ttgtctttga ttgaatcaaa aaccatagtg tttggaacta cattttcttt aaagaaagca
    12961 tccaaggctt ccttgtcttt atgaagcatg atttgtccat taacaggacg gttaatttcg
    13021 ttattaagac ggaagtaagt cacgtcttca agatgtttta atcccataaa atttccctta
    13081 tctaattaca aaagaaaggc ttctaagtta gccctaaaag cagtttcttc tggatgatgt
    13141 actaagatta tgctaattgt ttcagttttc ctggttggaa acctgaaaag acttcagttg
    13201 gtgtttggat aacaggagct gcgctaaaac cgagctcttt aacttgatcg acgtactcag
    13261 gttgctcatc aagattgatt tcacgataag agacattatt actgtccaag aaacgcttgg
    13321 tcattttaca ttggacacaa ttgtttttag aataaacggt taccattgtg taactcctct
    13381 tcaaaattta atactatctt agtatatcag aaaataaaat tttgtcaatg atttgaacta
    13441 aatatagtgg tgttcttttg ttgtttttat atataaacac aatatatagt gtttgtattg
    13501 tgaaatagca atgattatcc tataaattgt ttttaaagaa actatatcct gtgttttgtt
    13561 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
    13621 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
    13681 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
    13741 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
    13801 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
    13861 ggtgttatga gtcttggtgt tggccaaggt gctgacgtaa ctatctcagc tgaaggtgca
    13921 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
    13981 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
    14041 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
    14101 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
    14161 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
    14221 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
    14281 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
    14341 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
    14401 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctattaat gaagaagtga
    14461 ttgtgattgc gcatgacttg acaccatctg atacagctca attggacaaa aactttgtaa
    14521 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
    14581 ttgaaattgc tgctgtatta ggtacaaata acatcactga aatcgttaaa gacggtgaca
    14641 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
    14701 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
    14761 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
    14821 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
    14881 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
    14941 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
    15001 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
    15061 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
    15121 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
    15181 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
    15241 ctgaaggtgt tgcagttgcg gataacatcc aagttggtat catgatcgag attcctgcag
    15301 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaacg
    15361 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
    15421 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
    15481 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
    15541 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
    15601 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
    15661 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
    15721 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
    15781 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
    15841 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
    15901 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
    15961 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
    16021 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
    16081 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
    16141 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
    16201 aggctatgtc acttcacatg gcgaccacta tcattattac aatggtaagg ttccttatga
    16261 tgcgattttc agtgaagaat tactcatgaa agatccaaac tataagctaa aagatgagga
    16321 tattgttaat gaggtcaagg gtggatatgt tatcaaggta gatggaaaat actatgttta
    16381 ccttaaggat gctgcccacg cggataacgt ccgtacaaaa gaggaaatca atcgacaaaa
    16441 acaagaacat agtcaacatc gtgaaggtgg gactccaaga aacgatggtg ctgttgcctt
    16501 ggcacgttcg caaggacgct atactacaga tgatggttat atctttaatg cttctgatat
    16561 catagaggat actggtgatg cttatatcgt tcctcatgga gatcattacc attacattcc
    16621 taagaatgag ttatcagcta gcgagttggc tgctgcagaa gccttcctat ctggtcgggg
    16681 aaatctgtca aattcaagaa cctatcgccg acaaaatagc gataacactt caagaacaaa
    16741 ctgggtacct tctgtaagca atccaggaac tacaaatact aacacaagca acaacagcaa
    16801 cactaacagt caagcaagtc aaagtaatga cattgatagt ctcttgaaac agctctacaa
    16861 actgcctttg agtcaacgac atgtagaatc tgatggcctt gttttcgacc cagcgcaaat
    16921 cacaagtcga acagctagag gtgttgcagt gccacacgga gatcattacc acttcatccc
    16981 ttactctcaa atgtctgaat tggaagaacg aatcgctcgt attattcccc ttcgttatcg
    17041 ttcaaaccat tgggtaccag attcaaggcc agaacaacca agtccacaac cgactccgga
    17101 acctagtcca ggcccgcaac ctgcaccaaa tcttaaaata gactcaaatt cttctttggt
    17161 tagtcagctg gtacgaaaag ttggggaagg atatgtattc gaagaaaagg gcatctctcg
    17221 ttatgtcttt gcgaaagatt taccatctga aactgttaaa aatcttgaaa gtaagttatc
    17281 aaaacaagag agtgtttcac acactttaac tgctaaaaaa gaaaatgttg ctcctcgtga
    17341 ccaagaattt tatgataaag catataatct gttaactgag gctcataaag ccttgtttga
    17401 aaataagggt cgtaattctg atttccaagc cttagacaaa ttattagaac gcttgaatga
    17461 tgaatcgact aataaagaaa aattggtaga tgatttattg gcattcctag caccaattac
    17521 ccatccagag cgacttggca agccaaattc tcaaattgag tatactgaag acgaagttcg
    17581 tattgctcaa ttagctgata agtatacaac gtcagatggt tacatttttg atgaacatga
    17641 tataatcagt gatgaaggag atgcatatgt aacgcctcat atgggccata gtcactggat
    17701 tggaaaagat agcctttctg ataaggaaaa agttgcagct caagcctata ctaaagaaaa
    17761 aggtatccta cctccatctc cagacgcaga tgttaaagca aatccaactg gagatagtgc
    17821 agcagctatt tacaatcgtg tgaaagggga aaaacgaatt ccactcgttc gacttccata
    17881 tatggttgag catacagttg aggttaaaaa cggtaatttg attattcctc ataaggatca
    17941 ttaccataat attaaatttg cttggtttga tgatcacaca tacaaagctc caaatggcta
    18001 taccttggaa gatttgtttg cgacgattaa gtactacgta gaacaccctg acgaacgtcc
    18061 acattctaat gatggatggg gcaatgccag tgagcatgtg ttaggcaaga aagaccacag
    18121 tgaagatcca aataagaact tcaaagcgga tgaagagcca gtagaggaaa cacctgctga
    18181 gccagaagtc cctcaagtag agactgaaaa agtagaagcc caactcaaag aagcagaagt
    18241 tttgcttgcg aaagtaacgg attctagtct gaaagccaat gcaacagaaa ctctagctgg
    18301 tttacgaaat aatttgactc ttcaaattat ggataacaat agtatcatgg cagaagcaga
    18361 aaaattactt gcgttgttaa aaggaagtaa tccttcatct gtaagtaagg aaaaaataaa
    18421 ctaatgaaaa atgaaagtct cgataaagag gctttcattt ttattatgta tatatgtaaa
    18481 attcttgaca agcaatatta aaaagagtaa actattaact agttaattaa ccggtttatt
    18541 actttatagt gaatcaaata tacttaagaa aagaggaaag aatgaaaatc aataaaaaat
    18601 atctagctgg gtcagtagct acacttgttt taagtgtctg tgcttatgaa ctaggtttgc
    18661 atcaagctca aactgtaaaa gaaaataatc gtgtttccta tatagatgga aaacaagcga
    18721 cgcaaaaaac ggagaatttg actcctgatg aggttagcaa gcgtgaagga atcaatgccg
    18781 aacaaatcgt catcaagata acagaccaag gctatgtcac ttcacatggg gaccactatc
    18841 attattacaa tggtaaggtt ccttatgacg ctatcatcag tgaagaattg ctgatgaagg
    18901 atccaaacta ccagctaaag gacgaggata ttatcagtga aatcaagggt ggttatgtga
    18961 ttaaggtaga tggaaaatac tatgtttacc ttaaggatgc agctcatgcg gataatgtcc
    19021 gtacaaaaga agaaatcaat cggcaaaaac aagaacatag tcagcatcgt gaaggaggga
    19081 cttcaacaaa cgatggtgcg gtagcctttg cacgttcaca aggacgctat actacagatg
    19141 atggttatat ctttaatgct tctgatatca ttgaggacac gggtgatgct tatatcgttc
    19201 ctcacggcga ccattaccat tacattccta agaatgagtt atcagctagc gagttagctg
    19261 ctgcagaagc cttcctatct ggtcggggaa atctgtcaaa ttcaagaacc tatcgccgac
    19321 aaaatagcga taacacttca agaacaaact ggctaccttc tgtaagcaat ccaggaacta
    19381 caaatactaa cacaagcaac aacagcaaca ctaacagtca agcaagtcaa agtaatgaca
    19441 ttgatagtct cttgaaacag ctctacaaac tgcctttgag tcaacgccat gtagaatctg
    19501 atggccttgt tttcgaccca gcgcaaatca caagtcgaac cgccagaggt gtagctgtcc
    19561 ctcatggtaa ccattaccac tttatccctt atgaacaaat gtctgaattg gaaaaacgaa
    19621 ttgctcgtat tattcccctt cgttatcgtt caaaccattg ggtaccagat tcaagaccag
    19681 aacaaccaag tccacaatcg actccggaac ctagtccaag tctgcaacct gcaccaaatc
    19741 ctcaaccagc tccaagcaat ccaattgatg agaaattggt caaagaagct gttcgaaaag
    19801 taggcgatgg ttatgtcttt gaggagaatg gagtttctcg ttatatccca gccaaggatc
    19861 tttcagcaga aacagcagca ggcattgata gcaaactggc caagcaggaa agtttatcgc
    19921 ataagctagg agctaagaaa actgacctcc catctagtga tagagaattt tacaataaga
    19981 cttatgactt actagcaaga attcaccaag atttacttga taataaaggt cgacaagttg
    20041 attttgaggc tttggataac ctgttggaac gactcaagga tgtctcaagt gataaagtca
    20101 agttagtgga tgatattctt gccttcttag ctccgattcg tcatccagaa cgtttagg
//
LOCUS       DAVBCV010000039        19766 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBCV010000039 DAVBCV010000000
VERSION     DAVBCV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19766
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.039"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(481..1206)
                     /locus_tag="VQ516_001383"
     CDS             complement(481..1206)
                     /locus_tag="VQ516_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511814.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISAPYSE
                     LDYNKYWYVLHALIYMPMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIIFPNIMSFLLFLPIAIFM
                     QICCIRFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVC
                     IWIMGFEFSLLPKLIKLKEQNLV"
     gene            complement(1219..1956)
                     /locus_tag="VQ516_001384"
     CDS             complement(1219..1956)
                     /locus_tag="VQ516_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2511815.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNALLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            complement(1949..2677)
                     /locus_tag="VQ516_001385"
     CDS             complement(1949..2677)
                     /locus_tag="VQ516_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2511816.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSKNILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            complement(2711..2881)
                     /locus_tag="VQ516_001386"
     CDS             complement(2711..2881)
                     /locus_tag="VQ516_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511817.1"
                     /translation="MKKVNEYVISTAASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2924..5017)
                     /locus_tag="VQ516_001387"
                     /pseudo
     CDS             complement(2924..5017)
                     /locus_tag="VQ516_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(5010..5558)
                     /locus_tag="VQ516_001388"
                     /pseudo
     CDS             complement(5010..5558)
                     /locus_tag="VQ516_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5639..5701)
                     /locus_tag="VQ516_001389"
     CDS             complement(5639..5701)
                     /locus_tag="VQ516_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2511818.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            complement(5870..6858)
                     /locus_tag="VQ516_001390"
                     /pseudo
     CDS             complement(5870..6858)
                     /locus_tag="VQ516_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6807..7934)
                     /locus_tag="VQ516_001391"
                     /pseudo
     CDS             complement(6807..7934)
                     /locus_tag="VQ516_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(8097..8981)
                     /locus_tag="VQ516_001392"
     CDS             complement(8097..8981)
                     /locus_tag="VQ516_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET2511819.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(9093..9947)
                     /locus_tag="VQ516_001393"
     CDS             complement(9093..9947)
                     /locus_tag="VQ516_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2511820.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNLTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(10005..10763)
                     /locus_tag="VQ516_001394"
     CDS             complement(10005..10763)
                     /locus_tag="VQ516_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2511821.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYALS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            complement(10825..11013)
                     /locus_tag="VQ516_001395"
     CDS             complement(10825..11013)
                     /locus_tag="VQ516_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2511822.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(11335..11901)
                     /locus_tag="VQ516_001396"
     CDS             complement(11335..11901)
                     /locus_tag="VQ516_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511823.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKTYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(12169..13848)
                     /locus_tag="VQ516_001397"
     CDS             complement(12169..13848)
                     /locus_tag="VQ516_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2511824.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKVDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            complement(13940..14101)
                     /locus_tag="VQ516_001398"
     CDS             complement(13940..14101)
                     /locus_tag="VQ516_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511825.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(14785..15099)
                     /locus_tag="VQ516_001399"
     CDS             complement(14785..15099)
                     /locus_tag="VQ516_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511826.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            complement(15065..15454)
                     /locus_tag="VQ516_001400"
     CDS             complement(15065..15454)
                     /locus_tag="VQ516_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2511827.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            complement(15842..16636)
                     /locus_tag="VQ516_001401"
     CDS             complement(15842..16636)
                     /locus_tag="VQ516_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511828.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            complement(16636..17643)
                     /locus_tag="VQ516_001402"
     CDS             complement(16636..17643)
                     /locus_tag="VQ516_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2511829.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(17643..18650)
                     /locus_tag="VQ516_001403"
     CDS             complement(17643..18650)
                     /locus_tag="VQ516_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2511830.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLATFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(18640..19665)
                     /locus_tag="VQ516_001404"
     CDS             complement(18640..19665)
                     /locus_tag="VQ516_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2511831.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
BASE COUNT         6376 a         3631 c         2619 g         7140 t
ORIGIN      
        1 tggacatttt gaactaaaat taatttatta aacatatctc ataagcatat ctattatagg
       61 tgagacaata ttaagaaaga cggttaaaaa acaacctgac tccactggaa ttcaggaatc
      121 aggtcgctta agattttata ttattttact gtctacttga cagggagctg ttcaccaatg
      181 ggaatctata gctttttttg tgactgagaa ctattttatc tcagacttct tcaacacacc
      241 ttattattgg gcacttacgg aaggttaatg aattatttaa aataaaagta aagagaagca
      301 aacagacact ctatagctgt tagacgcttt ttagagtaat aacaatttga taaagtgaat
      361 acaacttgct taattaagcg taagaaacca gaatcataaa atcaattact ttgaagtcat
      421 gactcacgtt gtattgtttc attgttaatc gtattcaatt atttaatttt cgagcaacat
      481 ttaaactaga ttttgttctt tcaattttat tagttttgga agaagggaaa attcaaatcc
      541 cataatccag atacaaacta ataatttaat acctaagttt gtagttgact gaatttctgt
      601 ataataataa gttgcaggta taaatatatt ccaatcactc tgaaacgtga ttaaaacaat
      661 aagtatcagc aaaatattag gtaaggcctt tgctaaaatg gaatcaaatc ttatacagca
      721 aatttgcata aagatagcta taggtaaaaa taacagaaat gacattatat tgggaaatat
      781 tatattggga aatattatat tggttaataa catatcagtt cccataccac ctaattgaat
      841 taaagaaaaa attgtataaa caaaataaca aataaaacat ctcacgatat ttatcgttac
      901 ttttatatag atgattttca cgagattatt agtgttaatc accatgatat tccaaccatt
      961 attattatct tcaatcaatg cctctcttag attttgatac aaaatttgta taggaaaaat
     1021 cattggcata tatattaaag cgtgtaaaac ataccaatac ttattataat caagctctga
     1081 gtatggtgcc gaaatatcta aaatatatgt ttttatagct agaatgacta gaatctgaat
     1141 acagatgtca acaataataa ataaaaatat tttactctta atcaattcat ttttaagtaa
     1201 cttcattttt attacctatt aaagtttcat ctcaaaactt ctttcgttca atttacgaat
     1261 taatatataa aaaagtacga tgattattat attagatagt aaaatcatat aaaattgact
     1321 ttcagtcact ctaaaagcat gagaaagata agtaaatggt aagtaaatct taaaattata
     1381 atcgttgtag tatatgctaa ttgttaaaat catcattata ataccaattg gtattattcc
     1441 agaaattttc cttataagta ctaaagttag aaaatataat aacattatca tcaacgagga
     1501 aattaattga atggtaaatg tttttaaaaa gaaaatcaaa ccaatctcta aactgttatc
     1561 aatagccttc ccaataacgt ataagctaat ataatcagac aaccaaaaag taaatgtgta
     1621 aaccactgta atgagtaatt tagaatttat tgtgtacttg tttctaaatg gcaatgaaat
     1681 tacggttaac catgcatcat ttttaaattc tactgcaaac acttcgtaaa ttattaaaga
     1741 tagaactaaa taaaatagtt cagaaaaata aaatattgtt tgttctttaa aaattaactg
     1801 ccaattagat aaagcaagtt cattttgatg caccaataaa taacctcgat acctaaaagt
     1861 taagtctata tataacaaag cattcagaat tattggaaaa ataagaacaa accataagat
     1921 gctttttttc tgctttagaa actctaattt aatcatattc acctccagct aaacaagcta
     1981 agtaggcttc ttccagattt cgacaattat acttttgtaa aaggtagtgg caagaaccaa
     2041 aaaattcaac tttcccataa tttataatta taatatcaga tatcacttta tccagttcac
     2101 taagaatatg acttgatatt aaaaatgtaa tacctttttt ctcctgtaga aaacggatta
     2161 aacttctaat ttcaattaca ccctgtggat caagtccatt gattggttca tcccaaatta
     2221 atatttctgg actacctaac aatgatgcag ctatacttag acgttgtttc atgcccattg
     2281 aaaaattttt cacttttttc tttttaacat cttttaatcc taccattgat agatattcat
     2341 taagtctttc agtatctaaa ctaagccctt tcatatttgc tattaacttc aaatttttat
     2401 aagcatcaag atgaccataa aatgaaggtc ccaaaactaa tcctatccta gataatatat
     2461 ttttggaatt ctgttctacg gtttgattaa aaatagttat tttcccagaa tctggtttta
     2521 aaatccctag aatcattttg ataattgtag tttttccagc cccatttggt ccaacaaaac
     2581 catatacttt tcccttattt aaataaaatg aagtgttatg aataacttca atatctttaa
     2641 agctttttgt tagatttata atttcaacta tagccatctc tactccttag tttacagcaa
     2701 aaaattatgc ttaattctta ttgtttttgt aaacaatcag cgaacccaat actattccat
     2761 tcaacaaacc taaagaaata ccatattcaa ctggaaaatc taaaaaaatt gcaaatacta
     2821 ttccaatcat cacccctagt gaggctgctg ttgaaattac atattcattt actttcttca
     2881 ttttctttac acctcatttc ctttttgtat ataagctgta gtactatcca ccaagatcca
     2941 ttaagtactt atgatttcct gactcaacaa tcttaccacc tttcattaca aaaataatat
     3001 ccgcatcctt aatcgttgac aatctatgag ctataattat ttgagtacaa tcctgacttt
     3061 gtatatactt tgttattctt tcctcattaa tagtgtctaa tgcactagtt gcttcatcta
     3121 aaattacaat actaggatta tttattaatg cacgtgccag tgctatccgt tgcctttgcc
     3181 cacctgaaat atttgacccc atctctgaga tgatagtatt aaatttcatc ggcatagcca
     3241 tgatttcatc atagatttga actgctttac aaacttcctc tatcttttgt gaagtaactt
     3301 cgttctttaa agttatatta tcaagaatac ttcggttcaa taaaaatgaa tcctgtggaa
     3361 ctactcctaa attttgactt aatatttttt tatctatttg attaatattt acaccatcaa
     3421 ataaaatttt tcctgtatca atcttatata atcctgatag tatttttgat aacgtacttt
     3481 ttcctgaccc cgattgaccg actagtgcaa ttctctgtcc tggctcaata gtcaaattta
     3541 aattttctat tacaggagct gagtctttgg aataagagaa agataggtcc ttaatatcta
     3601 ttctaccttc taaagaacac tttttaagac cattttcaac attctcttca ttttcaagcc
     3661 agatatcatt tactctattt aagtatcctg ctgccaaaat atactacgta taagcattaa
     3721 atattgagat ttctgaacta aataaaattg atgccatcgt ttgaaatgcg acaacctcac
     3781 ccaataaaat ttttccattt aaaacattat caagtccaaa aatcaatata attataggag
     3841 cgaacaattg aaatacatta gtagctgaat tgtataaaat atgcacaata cttcgttttt
     3901 tatactttgt caatacgttt tttaaatact tagaccagtt ttcatatatt tcatcttcta
     3961 atcccgatat ttttattgaa gaaatagtat ttaatgcttc tgtttctact gagtaaatca
     4021 atgattgttc ttgtaattct ctattaaccg tatcatacat tatttttcta gtactaaaca
     4081 gaaacaaaaa attgattaaa ctcaatataa gagctataat tgataatgtt tttgaactaa
     4141 gaaacatata tattacaaca cttagaatag tcccgatatc tactactccc ccaacaactt
     4201 ggttagtaaa tagctcccta aaaccactaa gactagaaat tctgtataaa atatctcctt
     4261 ttgccctgag ttcaaaaaaa ctatatggta gtttaagcaa atgcttaaat gtaaaacttt
     4321 caatatctct actaaaaaat atattagaaa atagaattcc catactccta gccaagatag
     4381 aaataaggta caaacaaagt agagaaaata aaattaaaaa cgaggtttga aaagaaaatg
     4441 tattccctaa tgcagaatta ataaactttg aagtcattac tggtacggaa agtattatta
     4501 agtaacttat tattgaaaat agtaacgtca aaatcaatcg accttttacc tttgaaaaac
     4561 tagagaatac tcttatccat ggagaaggaa actgcgactt caaagattga taattttcgt
     4621 taggaaatga tagcagtact atattactaa aatgttttga aaactcctta aactcataat
     4681 ttatatatcc tttagaagga tccattatat agaccttatt cttttttact ttgtaaataa
     4741 ctacataatg ttgattatcc cagaatgcta ttaatggtaa cggaagttca ctaattttat
     4801 ttacatcttt aacttgatat gagccagtat caaaatttcg tttattcaat aactgtttca
     4861 agtcacctat actataacca tctctccctt tttctatatc acgtcttaaa tctagtaatg
     4921 tctctttgct tttatagaaa tgtagtatgg acaagcagca acataaacca cattctattt
     4981 gagacatttg ctcaatatta ggtacttttc tagtcatcat gtcctcttaa ctccagtaat
     5041 aaaacattaa gtaatctatc atcttgtttt cgagatattt catagacaac tccagatact
     5101 cctagcataa aagaaaatgt attcatagta ttcaaatccg tacttaattc tggtttgata
     5161 acctgagaaa cgacattatc aaaataaaaa tctatagatt cttttattaa taggttttct
     5221 tcatctttca atattgcatc aagacaactg atagtaccta cagaaccgtg acataaattc
     5281 aattctctta ttttttctac attagttaat acattttcaa ttattgataa atcattattc
     5341 ccaatattag ttttatcgaa aatttctttt ctagctaata aaatgcccgg agctccataa
     5401 caccaaaaat taggaaagga atcattatga tttcttcata tgtctttcca attttttaat
     5461 atattattga aacatttatc ttctttatat agtaattcct ctatacattt taatatatct
     5521 gtactaggat taatttcata ataatatgat aattacattg ctactccaga ataatcgtgt
     5581 gctaaacctg ttactgttat attatctaat tgtgaaatac aataattaat acactttttt
     5641 aaatgatgat aatatccatc accaaaacca aaatcggtaa aaattttgaa attttcttca
     5701 tacttggact tcctttccgt ttttatttat tgtacaataa ataaaaacgg atttaatttt
     5761 tgttgggaaa atgggaaatt atgaaatcaa aacttggcat cacacttaga aaagttcgta
     5821 aaggaaaaca aattagtcta tgctctgtag cagatgaaca tctttcaaaa tcacaaatat
     5881 ctcgttttga acgtggggag tcagagatat cttgtattcg actaatcaat attttagaca
     5941 aattacatat tactttgtag gggagttttt ggctatcgtt gctcaattgt ctgattaagg
     6001 tcctaccctt gatgaaacaa ttattatcca tgttttcttt attatagaca aagtaagaag
     6061 acgtttctcg aatgtagact ttatattttt tatgattttc ttcttccata atatccaatt
     6121 gatagttggg aatgaaaata agaccgctct gtttgacacc gaaagacacc ttgatataga
     6181 cgcccttatc aactagcttc tctatttggt tctctgcaag ttccacttca aattcacgaa
     6241 cggtatctca tttttcctta aatgtcttaa aggcttcctc aatctcttca gtggatactt
     6301 tatccttatc tcgttcttct tggaaagcat ggtactgttc ctgtaaattc tctaatcctt
     6361 ctgaagcaac gacttcctta tttttaaaat aatcttgaaa aaatttgaca tcatataatt
     6421 tcttatcact tattttttga tgacccaaac ttatcttttg attattttct tccaggataa
     6481 aagttacatt ttttgtttta agtcaatggt tagattcaat tcttttgctt ttgttattaa
     6541 atcttctaaa gaattgacac ggtttaacaa aaattctaaa cgactttcaa tctcttgctt
     6601 agcaaaatgc gttctaaaaa attcttcatc atatagatct cgtttgctga gttggcgccc
     6661 tcgaattggt tttatcatcg ttctatctgt catcaaaaaa cggctatgct tttgactaaa
     6721 atcaatctga acatgcaact gctttgcttt ctctaaaaaa tcatcaaacg atttagattg
     6781 ctgaagcaaa aaataaagac gttgtttcaa ttcaaattta tgactagatt ccttatattt
     6841 tttataatct cgaaaagatc aggattttta gctaaaactg ccttacgaaa atccttccag
     6901 aactgatggt caatctcatt agccacatcc aaacgccaag catcgatatt aaactcttca
     6961 atccaataag tcgcaacctt taaaagataa ttcttgacct ctggattggc tgtatttagc
     7021 ttaggcatat agtcctcgaa accaaaaaca tgatagggta agtctctctt attaactagc
     7081 ttttcagttg tcaatgggaa ttgttgaata tggaaccaat ccttataagc agactgttca
     7141 ccatttttga cgacattttt tcattgaaga gattgcgaac caatatgatt aaataccgca
     7201 tccagcatga ctttcatgcc acgatgatgc gcttgatcca ccagttcccg aaaggtctcc
     7261 ttgtctccaa aataacggtc aatttcaaag taatctgtcg tattgtactt gtgattactt
     7321 gtagattcaa agatgggaca aagatatagt ccagtaatac ccaagtcttg caagtaattc
     7381 atatgatcaa taatcccctg taaatcacca ccaaagaaat catcgctctt aggtgtgaca
     7441 gatgaatccc agtctaaagt cccttctggg tttaatagag cattgccatt ggcaaatctt
     7501 tcaggaaata tctgatacca taccgtattt gaaacccagt caggaacctt gcaggcatca
     7561 atctcatgaa gataaggcaa cttaaatcca ttcccaattg catgaagatt ttctagagaa
     7621 ttttccacac accctttatc gccatacaaa atattttgac cttctgtatc tctgagctca
     7681 aagagatact ggatacgtgc aaagtcaact gacacttcaa cctgccaatg gtcaaataag
     7741 gtaccagaag ttatcttgac catttctttt gtatcctgat aaaactcttc cataaagata
     7801 aaagggtccc catagtgcaa gttgatgctt tcaatgtccc ctttcttagt tcgaattcga
     7861 atatggagtt tcttatcctt ataaagatag gcatactccg actcaggcct atggtaaata
     7921 gcactcaatt ccatcaattt gtctcctaaa gtattcattc aaattttacg caaacgtttt
     7981 caaactcaat aaatccagta tactattttt gtaaactatt tgcaaggaat gtccgttgtt
     8041 aaaggaacta tcttttaaag tatacaaaaa gccttaaaaa aggctttctc tatatactaa
     8101 tcctctttcg gttttgaaaa tacttctact cgctgagcta ggactttgat ttctacaggt
     8161 aggttatctg atttatctcc gtcaacatct gactccaatt cactatctga agagatttta
     8221 atattacgcg ctttgatata ctcgatatta tcatttgcga caacatcccc ttttaataaa
     8281 tcaggaatga cggataattt ggagaatata gaggcatctt tcagaatcaa aatgttggca
     8341 tagccgtcct tgttttcttc aaagattttc ttatcagcga agtaatttgt caaaaggacc
     8401 aaaacatggc tagcttcacc aacataattt ccattttctg tctcaacctt aatgttaaag
     8461 acctgatctg tcatgacaga cttcatggta tttacagcat aggttaaaat accgaatttt
     8521 gttttgtcct ctatttcaac attgtgaatc gcctcaggca gagaaccgat actaaagata
     8581 taaccaaaat agttgtcatt tgctttaccg atatcaatct tattggttaa atcaaaatcc
     8641 agttcgtcaa ttgcgccatc gatgtcttga ttgatttcca aaagttttgt aatgaggtta
     8701 cccgtaccgc ctgggataat ccctaactta ggaatgtagt ctctctcatc aatacctgaa
     8761 atgacttcat tgacagttcc atctccacca aacacaacca ctgcatcata ctgctcacga
     8821 gaagcttctt cagcaaaatg tgttgcatcc agcgcttttt cggtaatttt ggtttcaaca
     8881 tattcaaagt attcttttgc tttattctcc agcttttctt tgtaatccaa agccttctcg
     8941 ccaccagaag tagggttgat aattaccatt gcttttttca ttgattttat ccttaatttt
     9001 aaacagaaat gtttacattt cgtcgtatgc aagtaaatgt aatcctatta tacaatgaaa
     9061 atacagaaaa gagaaatctg acgtactgga gattaatacg cttttattct attttcccat
     9121 cgcctaacta catcctttaa gggttcatcc aagtaagaat aggccttatc cttgatccaa
     9181 tcaggaatac cgtaagctgc ctctgctatg ttacaagtga ttgctgcgag agtatcactg
     9241 tcgccaccaa gtgagatggc atttcttatc gcatcttcga agtctctact ttcaagaaag
     9301 gcgataatgg cttgagggac agtttcctga catgtttcgt taaaacgata gttaggacgg
     9361 atttcatcta aagtttgaga tagattgtaa tcgtattctt tttctatata atccttaatt
     9421 cgtcttttac actctgtaag attgtcgtta attgattgct cgtactctct acaataacca
     9481 ccaaagtaaa aacgacatag aaagatagca tcagctgtag ccatagcgcc tttgacacct
     9541 tctggatgat tatgagttac ctctgcagaa agactcgtaa gtcctctaga tgatggccat
     9601 ataccagttt tcgcataaaa accacagtcc atgatccaag cacatggaga aatacgcata
     9661 gctgatccat tcccaaagct attataaggc tcacggttat cgctgtttag ccatgcatta
     9721 aaccgagcac cgtaatcagc attcggatac attctgccat atttcttcat cgcgtcaatg
     9781 aaatcgtctt tttgtccacc attcataatt gcttctgcaa cagcacaggt cataaccgtg
     9841 tcatctgtaa aaaagcagtc cttccgaaat aaaggaaagt cctttgtttt gatattgttc
     9901 cattcgtaaa cagaaccgac aatatctcca ataattgctc caagcatcag attcctcctt
     9961 gttcattttg atgcttttta tattggttat ataccatatt tattttagaa aacaacatcc
    10021 tgttggattt taaaaatttc atttttttca aaatagggtt ttaccatttc tttccaccta
    10081 gctctatgaa aattgattga ttttaaagga gatagggcat aatttcccaa tgcataacca
    10141 tcatttactt caacaacaag tgttctgcca tcgcgagtaa caccgatatc tagtccataa
    10201 gctattggcg catctttcca acatgatatc gcttcatcaa ttacacttgc atcaaattgt
    10261 gcatgataat cacctgtata gggtcgaaca tctaatacgc gaccatctaa cacaaaacaa
    10321 cgccattcag ctatgaattc tacaacctca ctaatccata taggatagtc gaaaggtaga
    10381 ccaataccta ttaaatcatg ggttccatta acaactcttc cagtaaagac ttttgaacca
    10441 gctttaggct taataaattt tccccaatta tcaggtatat tcacaatctc tcctaaaata
    10501 ccagcataaa tctttcgacc ataaaactct ttaagctcaa taggatagtc ataaaccgga
    10561 acgtttaagc ccatcatttt tagtaatgct ctagtctcca ttatataatc tacaactata
    10621 tcttcacttg ttaactcttt tatttcagaa aaagattgat ataaaataac ttcttctcct
    10681 tgtaagtagg cacctacttg agcattgtat ttattaattg aaacctcact tggtaattta
    10741 ctttgtctaa tataaacaac catttcatca ctcctatatc actagtgtta cactgatttg
    10801 taaaaaataa tagcaatttt gctcttattt ttttgagtaa atagctccca taatatcatc
    10861 gaaataatca acggtattta ggagtaattc aataacctgg gactttgtta gtcgcattcc
    10921 ccttctatct ctagcatctt ctactaaatt ttcaagtttc tctagatttt tatcatccaa
    10981 gctaatcatt attctatttt tatcggttgc cattttcatc acctcaagtt aattctatca
    11041 caggtgtaac acttgtgtca actggctttt ataatacatt agtttaaaag tggagaggat
    11101 ttttaacaca gtaactttaa atctttggta ttaaaaaatt ttcacaatat ttatagaaat
    11161 aaaatctgtc tcaaatcagt tatcaaatct agtataaatt atgagcggct actctaatac
    11221 tttccctcta aacaagaaaa agacttacac tcaaggattt tcttcccccc cttcgttata
    11281 acgttttgac tcttttacta gcaaaggtat atactcacaa ggaactttgg ttgactattg
    11341 aatctctcca acttcttctt taacatatcc ttctacatct tcaatctcta caaacattgg
    11401 gtctaagtga cacaagaaat gccaaacttc gatccctttt tttctgtaaa gaatcgcttc
    11461 accgtcttca cttccgaaaa agcttctgtc gatttcatat ccgcggcttt ctaagaagtc
    11521 ttttgcttta cgatagttcg tttctcttgt ttcgacatag gttttaactt catggttgtt
    11581 aacgacatat gcatcaattt ttgaatatcc ttcgatcact ctatcatttt tgagggataa
    11641 atttgaaatc tctttccaaa taatgtttac attttcctca ggatcgaaca taaatttaga
    11701 taaaggaaca atatttccgt taaaaataat ttccatataa tccggtatgt ttttaggatt
    11761 aaaatactcc acttcaaaac catcttctgt ttccagagtg tatcccggga tttgagctac
    11821 aaaggctttc ccatcttcta tggaatcaaa tgctactaaa tctttagaat aatcattttg
    11881 gtacaattcc aatataacca tcgataatct ctccattttc attatcaggc taatgtaaat
    11941 aagcacgtca cctgaccaat tcaggctctc tgtatcatct catcatattt cctacttact
    12001 ttacgagtcc tatacccaga acacacctta tcgaccttcg gtctcacctc gttgcattgg
    12061 ctgaacatct acttttactt cgctgatgct tcaactcgta caagcagtga taccgcctca
    12121 gcgtgatgcg tcagtgggac tcaaaaggtt cggggaacct tttgaggatt aactacgttt
    12181 ctctaataaa cttacacatt caacttgttc atcattgtcc aaacctatgt tgagattttc
    12241 ttctataatt ggtagcttaa aagtaatgga ttttagccat tgtccgttag attgtttttc
    12301 ttcataaact tgaatttcag aaatcaaagc tgaaattaac tgcctacgct ctacatcatt
    12361 catgacttta tagagcttat caaaatagat cagaacctta tatatgttat ctcctgtaag
    12421 cttttcagct tcaatagtct gtttctttgc tttcgcatca attagtgatg attctaattc
    12481 atctactttg tcatacatac gatatagtct atcatctaaa tcctgtttcc ttctcttata
    12541 atgcttatct tcaacatcta aattatctat ttcctcaatt agcttaaact ttgtagaatg
    12601 actctttctc aattcctttt ggtaattatc tatttctttt tctatttcag aggtatccac
    12661 cttcatgttg attttttctt gcatcataga agcaaatttc ggattactta ctatcttgac
    12721 aatcacctct gcaacagcat catctaacaa ttcttctcta atttgcttac tgaatgtaca
    12781 cttatgacct cttatcatct gcctatggtt acaaccatag taataaaaat ctttatactt
    12841 tgtgccatct ttctttttct tgatacactt gttcccaaac attcccactc cacatatcgg
    12901 gcattttaca attccagaaa gcaagtgtgt gcgtgtatct tttcctttat tcacatgctc
    12961 atatttcttt gcttgagatt ttagcttaac ctgagcagct tgccaaactt catcggaaac
    13021 tatagcttca tgtatccctt cagatattag atattcatct tgttcaacct gcttatattc
    13081 atttcttgta ccatgaactt tttctaaagt tcttcttcca aatgctattt tcccattata
    13141 tacaggattc tttaatatct ttcttataag acctgcatca aacaaaggat tcttaccatt
    13201 ctgtcttggg atttttctaa ttccatgatt ctctaagtat ttagatatcc cattggctcc
    13261 tatcgtagta tttacatact ggtcgaaaat cgttcttatt gcaactgcct cttcctcatt
    13321 tataaacagc ttgccgtctt caagtttata tccatacgga gcaaagccac cattccattt
    13381 tccttccctt gctttttgaa tgcgaccttc cattgtttga atacggatgt tttctctttc
    13441 tatttcagcc acagctgata aaacagaaat cattagtttc ccagcatctt tagatgaatc
    13501 aatgccatct tcaacgcaga taagattaac tccataatcc tgcattatct gaagtgtaga
    13561 aagaacatca gcggcatttc ttgcaaatct tgataactta aacacaagaa caaaagatac
    13621 tccatctttt ccagatttta tatcttccat cattcgattg aactgtattc taccttcaat
    13681 agacttgcca gacttcccgg catcttcata ctctccaaca atttcataat cgttgtaaat
    13741 agcaaaagct ttcattcgtg atttttgtgc ctctaacgaa tacccctcta tctgtattga
    13801 cgtagatact cgtgtataga ggtatacttt tattttttct tttgacatag tattaatctc
    13861 aatataattt ttctatatca tatataattt ttttaattta agtttggact atcatttcaa
    13921 gtatattata acacttttat tagtccgtct caatttgtgt ttttgccatg tcaaaactat
    13981 ttttcatctc ttgatttttt gctggcgttg gatcgggtag attatctaaa tctaaagcac
    14041 cagcatattt tgcaatcaga tttgctatta aatcagccaa tccattccag tcattgtcca
    14101 atatatacct cctctaaagt tttatatcta ataattattt gtttaattaa gttttttgac
    14161 attgacaagt gctttggatt agcaacatag gaatctcacc tccgcctcta ttccggatga
    14221 gccggcttca accttagaag tatcattatc ctcattttct tcatagcgga tagggtatcc
    14281 ctccctatat tcaaactctt acttatcgct cactttcttt ttacttagca gaactttttt
    14341 tgccgaatta ttcagccgaa agatcttgac ggataggtta ttacgctcca aaaataatta
    14401 acgtcttgtc ttggtctatt caattgttaa ggttcaaaat ttatcgagag ttattaatct
    14461 ttttaaaatt tgaccatcag aaaatattta tcttaatgta acaaaattct ataaattacc
    14521 ctcttatact taacagtgaa aagaagtctt tcttggtaac caattttgaa atagaatttg
    14581 cttatataaa aaggtcaaat tcccactgca taagtagcag tgaaaattag accctcttgg
    14641 taactgtcat ctaaaagtct tctagcattt ctttaatttt ctaatcgcct ttaaagtgtc
    14701 atatttatat ttttttatct ctggcatact ttgatacctt tttagactta aagtctttaa
    14761 tagtgccttt ccacctcttt ttatctataa agattctcct acatcataat tcattttttt
    14821 atttaaacct ttctgtctta gtttgtcttt atcttcttca taccatttta agattgtcac
    14881 atagtggttt tgataggtct taccactgct ttccatgtat ctggatagtt tatttatcat
    14941 tatatctgtg tgtgagttta atttttcttt tagattttta tattcttctt tgcttaacct
    15001 tacatttttg aattctccat aaaaaatggg ggtggacttt ttatctatct ctccctctct
    15061 ctctttatct atctctatat ctttccatgt aattccaatc tggagtacct ctactgtcta
    15121 tcggtaattt aattttgata tctggcaata ctgtgctaga tatttgatct ttatattcag
    15181 tattttttaa agcttgccta ataattgaag ttaaatagaa tgctacttct ttattcaatt
    15241 ctttattttt taattttaaa caatgaattt tcatatctag gcttgcttta tatttatgat
    15301 aaaagactgc tcctaaaaat gaaacagata taaaattttc aaaaactcta taatttttat
    15361 catctatatc ttcgtagtaa cctaagatac cattgtcaat atttgtagca ctaattctag
    15421 gagtttttcc atcgagtaaa tatctttttg gaatagatga gcctgttggt acttgactcg
    15481 atttcccctt tttttcggta ataaatattt ctttttattt tgttgtctga tatttttcct
    15541 acctgtcctt tgtaggatga gtattttcta gattttcttg aataactttt tacttgaagt
    15601 tttagctttt gaactagtcg ttgtactttc tttttgttta ttatcagtcc tgatcttttt
    15661 aatattgctg ttattctcta tatcctattt ttcattcatg atattctttt actaatttta
    15721 tcttaaattc tgtgctgtat ttgccattaa aaaactgacc tcctttagtt agttttttgg
    15781 cctaactttt gagggtcagt tcaaaatttg cgacttttaa atgaattcca atattcaatt
    15841 attaagagct aacatggtgc ttgccaatag gaatcattag aggcgaattg gaaatagggt
    15901 cacgtataat ttttgcttca agattaaaga tatctttaac tagtttatca tttagtatat
    15961 cttcaggctt tccctctgca acaagtttac cttctttaat tgcaaatagg taatcagcgt
    16021 atcttgctgt tagatttata tcgtgcaaaa tcatgcaaat ggttgtctta tatttttggt
    16081 ttagatcagt caagaggtct aatagttcta tttgatatga gatatccaag taagtagttg
    16141 gctcatctaa aagtaggata cttgtatctt gggctagggc tagagctatc catactcttt
    16201 gcctttgacc cccagaaagt tcttcaacta ggttatttgc tagatcttca acattggcct
    16261 taaccattga tctgtttatt atttcaaggt catcttttcc aagactctta aaaggctttc
    16321 tgtaggggaa acgaccacgg cttacaagat cagctactgt tattgattca gggattattg
    16381 gagattgagg taatatagct atgtgttttg ctaaatcttt ttctttataa gaattaattg
    16441 atttattatc aagcaatact tctccctcta atggctttat aagtcgagac aaggttttaa
    16501 tgagtgttga tttcccacaa ccatttgacc caataataac tgatattttt tcttcaggta
    16561 tttttatatt tatattttcc aagattattt tttcatcata accgcaggta agattatttg
    16621 accacagacc tttcattata tattcctcct gttcattttt attagtaagt atattaagta
    16681 tggtgaacct aacaagccag ttacaacacc tactggatat ctagctggta aaatattttg
    16741 agagaatatg tctgataaca aaactagtaa aattccaacc aatccagcta atattgggct
    16801 tcttttcttg ccaatattta aggctatggg accagctaaa aaagatatac aagctattgg
    16861 tcctgtaatt gaagtagaaa aagcagttaa agatacagcg caaaaaatta aaacaagcct
    16921 tgaaagctcg ggatttgctc caagtccgat tgctatttct tcaccaagtt caataatttc
    16981 tagtctttta ttaaaaaata aaactaatat agtagcaata atacttacta ttagaacaag
    17041 aggtatgtca tctaactttg taaaagataa agagccactg agccatctca taacttcttg
    17101 taattcatat cttgctactt tcaacaataa aaatgaggtg cctgctcttg tgacagcttg
    17161 aaaaccaata cctaatatta tcagtcttgc tgctgaaaaa ccatcttttt tagctagtaa
    17221 aaataatatt aaagatgatg ttagtccaca agttattgaa ataattccag tagttaaact
    17281 atttgttttt aataccaata tgcaaaagac cgctgcaata gatgaagaac ttgtgacacc
    17341 gattatatca ggacttgcaa gaggatttct taacatagtt tgaaagataa atcctgccaa
    17401 tccaaaagac cagccagcta taattcctgc taataatttt ggtaatctaa tttccataat
    17461 cgaaaaacta gctccaggaa cagtttcact atttaagact ttaatcaaag ttgaaaaaga
    17521 ataactttca tctccgataa gtaaaatgaa aaatgataga ctgattatta ttaataaaaa
    17581 tagtgaggaa aatagtgtta ttctattttt tcttttttga atacctataa ttaaattttg
    17641 cattagttat taacccctct atttttcata gttacataaa taagtactgg acccccgatt
    17701 attgcagtaa ttatccctac ttcaatttca cctggtttac ctaacatacg gccgattata
    17761 tcacatataa gcaagagctc tgcacctata aaagatgaag aaatggtcat tgtgcgtata
    17821 tctttgctta taaataagcc acaaaagtga ggaactataa gacctacgaa gccaataggt
    17881 ccaccaattg cagtaatact tgaacataaa agcacacttg caattattgc aagtgatctt
    17941 atcctattaa cattaactcc aagaccaaca gccatttcat cacccatagc taaagcgttt
    18001 aaatctgatg aaataaatat agctatcaag tgacctaaaa ttataaaagg tagtagtgta
    18061 gatatagaag ataatgtagc tgctccaagg ctacctattt gccaaaatct aaatttgtct
    18121 aagacgttat tattcggtaa aattaaaaaa cttacaaaac tgcttaaagc catactaaca
    18181 caagttcctg ataaggctag ttttataggg gtaaggcctg cttttccgct tacagcaatc
    18241 gcgtatacaa aaattgcact tactaagcca ccaatgattg caaaacttat atggcttatg
    18301 cttgatgaaa ttcctaaaaa agaaagacca attactacac taagacttgc tcctgtgttt
    18361 ataccgagta tacctggatc agctattggg tttctagtaa ctgattgcat caatacaccg
    18421 cttatggcaa gactagaacc tgctaaaatt gcaaaaatag ttctaggtat tcttttatat
    18481 ataattgatt taataaaatc atcatttgta ttgcccattc caaaagttgc taaaaaatct
    18541 ctgatattaa tttcttttgt tccaagcttt aatgacaaaa atattgtaat aagaagacct
    18601 aggcagatta caaaaattat gcctaggttt atttgttttt tatttcgcat ttttgcatgc
    18661 atttcctaaa agatttaggt attcttcaat agtatagttt attgaaagtg gtgttggagt
    18721 gcatgaggct gctaacggtg tattatctgg aattacagca acggcaccat ttttaattgc
    18781 atttatttta cctaaaagag gatctttttg taaagcttca agagttttat catcaccata
    18841 agttatgatt acatcagcat catttatctt atttgcttct tctgcagaaa tttcctttgc
    18901 aaaactatct tcactctcaa attcttttaa tgattcaggg aaaactagac ctaaatctgt
    18961 taaataattt gctcttggat ctttgctagt ataaatccag aattttgatg tatctgcagc
    19021 attaatcata gtaaataata ctttttttcc tttgattttt cctttgattt ctggatgttt
    19081 ttctaattct ttggatatac gagcttcagt atttttgatt aactcatcac cttctttttc
    19141 catacctaag gcttttgaat caattttaat catatctctc cataaagttt gccaaggttt
    19201 agatttgtat gctgctacag gagcaatttt tgatagagtg tcataatctt ctttagttat
    19261 accagaataa cctgctaaga taacatctgg tttagaattt gatattgctt caaagttaag
    19321 tccatccaaa tcgtcaaata ggttagcttt accatttagt tctttgattt tttcttctgt
    19381 ccatggtaaa actcctttat cagcacttac accgtaattt gcttttgaaa atccaacagg
    19441 aactattcct aaagctaatg ctacatcatg atttccccaa gcaatagttg caactctttc
    19501 aggtttttta tctaatatag tttgaccgaa agcatgatct aaaactattt tatcaggcgc
    19561 atgctcttta gaagtatttt cttcattttt aacagaatta gaagaacaag cgctaaaaac
    19621 tatacacata gctaaaatag ctattagaaa aaatttgttt ttcataaaaa ctccttaaac
    19681 atatttcaag tctattgtat cgataataat tatcattgtc aatagataca gttacaaatt
    19741 taataatata attttttcaa aaaagg
//
LOCUS       DAVBCV010000040        19567 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBCV010000040 DAVBCV010000000
VERSION     DAVBCV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.040"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..647)
                     /locus_tag="VQ516_001405"
     CDS             complement(<1..647)
                     /locus_tag="VQ516_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2511832.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(756..1274)
                     /locus_tag="VQ516_001406"
     CDS             complement(756..1274)
                     /locus_tag="VQ516_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2511833.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1501..2370)
                     /gene="rsmI"
                     /locus_tag="VQ516_001407"
     CDS             complement(1501..2370)
                     /gene="rsmI"
                     /locus_tag="VQ516_001407"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2511834.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2373..2690)
                     /gene="yabA"
                     /locus_tag="VQ516_001408"
     CDS             complement(2373..2690)
                     /gene="yabA"
                     /locus_tag="VQ516_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2511835.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2730..3620)
                     /locus_tag="VQ516_001409"
     CDS             complement(2730..3620)
                     /locus_tag="VQ516_001409"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2511836.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3617..4255)
                     /locus_tag="VQ516_001410"
     CDS             complement(3617..4255)
                     /locus_tag="VQ516_001410"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2511837.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4475..5272)
                     /gene="proC"
                     /locus_tag="VQ516_001411"
     CDS             complement(4475..5272)
                     /gene="proC"
                     /locus_tag="VQ516_001411"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2511838.1"
                     /translation="MKIGFIGLGNMGASLTKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(5276..6538)
                     /locus_tag="VQ516_001412"
     CDS             complement(5276..6538)
                     /locus_tag="VQ516_001412"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2511839.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6548..7678)
                     /locus_tag="VQ516_001413"
     CDS             complement(6548..7678)
                     /locus_tag="VQ516_001413"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2511840.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMVEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRFQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7761..9641)
                     /gene="cbpE"
                     /locus_tag="VQ516_001414"
                     /gene_synonym="pce"
     CDS             complement(7761..9641)
                     /gene="cbpE"
                     /locus_tag="VQ516_001414"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2511841.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SE"
     gene            complement(9644..10531)
                     /locus_tag="VQ516_001415"
     CDS             complement(9644..10531)
                     /locus_tag="VQ516_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2511842.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10521..10982)
                     /locus_tag="VQ516_001416"
     CDS             complement(10521..10982)
                     /locus_tag="VQ516_001416"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2511843.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMFLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10979..11887)
                     /locus_tag="VQ516_001417"
     CDS             complement(10979..11887)
                     /locus_tag="VQ516_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2511844.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11880..12077
                     /locus_tag="VQ516_001418"
     CDS             11880..12077
                     /locus_tag="VQ516_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2511845.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(12129..12800)
                     /locus_tag="VQ516_001419"
     CDS             complement(12129..12800)
                     /locus_tag="VQ516_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2511846.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12854..13075)
                     /locus_tag="VQ516_001420"
     CDS             complement(12854..13075)
                     /locus_tag="VQ516_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2511847.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13347..14156
                     /locus_tag="VQ516_001421"
     CDS             13347..14156
                     /locus_tag="VQ516_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2511848.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14337..15212)
                     /gene="aguB"
                     /locus_tag="VQ516_001422"
     CDS             complement(14337..15212)
                     /gene="aguB"
                     /locus_tag="VQ516_001422"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2511849.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKLLAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15222..16307)
                     /gene="aguA"
                     /locus_tag="VQ516_001423"
     CDS             complement(15222..16307)
                     /gene="aguA"
                     /locus_tag="VQ516_001423"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2511850.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16304..17431)
                     /gene="nspC"
                     /locus_tag="VQ516_001424"
     CDS             complement(16304..17431)
                     /gene="nspC"
                     /locus_tag="VQ516_001424"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2511851.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17431..18690)
                     /locus_tag="VQ516_001425"
     CDS             complement(17431..18690)
                     /locus_tag="VQ516_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2511852.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMLD"
     gene            complement(18687..19547)
                     /gene="speE"
                     /locus_tag="VQ516_001426"
     CDS             complement(18687..19547)
                     /gene="speE"
                     /locus_tag="VQ516_001426"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2511853.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEYDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         5673 a         4504 c         3479 g         5911 t
ORIGIN      
        1 taggattcta gttttgcagg aagtctaatg taattctcaa gctgtgagtt cattatgatt
       61 tgtaaatccg atatatcttc agcagctaaa aacacatttt gaaatgttcc aagtccgttt
      121 tcaccaaaac tatattctct cttactatac ttactcttat tattctctat atagttagag
      181 tcaaggttct taacctcttg aagttcattt tctttacttc tgaggttaaa atcttttact
      241 tctgaagtta agttttttga cttctgtaag tcattttctt ttacctgaaa tatactcata
      301 aagtctttaa cataaatgat gttcggctta ccaagtccaa gccttactct ttcgatcagt
      361 cctattcctt tttttacatc aagctcatct aatgttttta tggcagttgg ctttgagata
      421 tttcttcttt tcataatttc ttcgacagta aaatagataa atactctgcc ttccttgtct
      481 atccagttat tcttaaatga cattcctgtc tgtttaagaa gtatggaata aaggataatt
      541 gcttctgctg ataagccccg aaattcttct ctatcaacta atatctccgg tacttttaag
      601 aagttaaatc tttccgcttc tctgttataa aaatattcaa agtccatgca agatcacctc
      661 ccttcttaaa aatttgcaaa gaaaaagacg atagtttttc ttctatcgcc tttggtaacc
      721 atttttatga aattttttaa agttaaaaat ataatttata atactatctt cttccatttt
      781 ctgattttag ttctgaaagt tccaaaaggt gcaaccgtat ttacatgaat aaacttatac
      841 acttcccaag tcgctgtttt agtcgcttca tcagcccact ttctcatatg cggttcaaat
      901 aattcttcct cactcaacga atcaatcatt gcagagatag aattaatatt ttcatttaat
      961 tttgctttca actcttgcag agataaatga gcgtaggtat ctgtgaacca ctgatataat
     1021 tcaccaagtt gattccattt aaatttatcc gatggtgttt ttacttgaag cccctttctt
     1081 tcatcttctt cccatttaag aaccaagttg gtccaaccaa cctgataagc aaggttttct
     1141 gctggagttc tgtcaacttc atcagctctc ttatctttta aattttctgg aatattatca
     1201 aattctaaaa tatatttctc aaatgttttc tctatctcag cttttagttc ttctttattt
     1261 tcgtatgttc gcaaaaaatc accttctttc tgatttatca atcctttgat ttaaaatgac
     1321 cttatttttc tatttatacc ttctaagaac gctataactg ctgctccaaa catcggtatt
     1381 ccgtatgggc ttaataaaaa acttaatacc aaagcttcta taccaagtga tacatcttta
     1441 tattatcatc actaattaag ttaaacagaa acagacagaa ttattacatc ctgtctccct
     1501 ttattgtttt tcttcccaat cgtggtaggc agcgtagagt tgactcttat tccactggta
     1561 aatcttagct acttccttaa tagcttgatt tttcttcatg ccttgctgga tacgggcttg
     1621 gatttctaag aacaagtcct cctcgtcttt ttcctccaca tcctgactgg caccttcaac
     1681 aataagaagg cattcgccct tgagtggcgt ttcagcgatg ctttccagta attcagaaat
     1741 tgtacctcgt tggtattctt catagatttt ggtcaattcc ctgaccaaaa caaccgagcg
     1801 gtcaccgtag acttctaaca tattttccaa cgtgtctgct acacgatgag gtgattcata
     1861 aaaaatctgt gtttcaggat aatctttttt agagccaaaa aattgcttct gttgacctga
     1921 ttttctcggt aaaaaaccgt aaaagatatg tggctgtggc gctaaaccac tggcaatcaa
     1981 ggcagaaatt cctgcagagg tacctggaac agtcacaact gcaatttctt cctcaatagc
     2041 tgccttaact aaatcatgac cagggtctga aatgctaggc aaaccggcat cagagacctg
     2101 agcaatactt tgccctgctt tcaagaaacc aatcaaatca ggaatttttt ccttggcatt
     2161 gtgctcatga aaactgatct gcttggtgga aatgtcaaaa tgcttgagca aaagccctgt
     2221 attgcgcgta tcctcagcag caatccagtc cacttctttc aaggtctgga tagcacgaaa
     2281 agtcatatca tctagattgc caatcggcgt tgccactaga tacagcttgc catagggaga
     2341 ctgcccctta aaactttttt gaatttgcat gcttactccc tgtataacaa ctcatcacag
     2401 aacatacatt cttcgtcctg ctctcgacgt tgtccataaa aatcattaca tacgtgaaat
     2461 ccatcacggt aaatacgacg gacactttcg cgaacatgct tggccttgac gggagcgtct
     2521 gcttccacct cacccaagcg ttctcgcaac ttactatttt ccaagcgaag agctgtattt
     2581 tcttctacca agctcttgag atttttcttg atggcttcca catcggctaa agttaccaat
     2641 aattgttggg aaaaatcatc cagcgcgtca aataattctt ttttgtccat aaaacagccc
     2701 tttcctttct ttatcattca tttttgagtt tatatttctt tcaagaccag atattccatg
     2761 gcattttgaa agctgacatt agcttgccac atttttctag cttctagtaa atcttgtaga
     2821 atcactctta ctcttacctg caagaggtcc tgcccacaga gaacttcaag aatccgtaaa
     2881 acctgatcct gtttttcctt atcatctgcc aagttggcta atttggcaac ctgtagataa
     2941 ctttcttttt tcttagctac taaccaagtc agcaggcgtt cactttcatc gaccaaggtc
     3001 caaaaacttg cctgattagc caacttttct gcttcagctc gcgattgact aaacttagct
     3061 aaaagagtcg cttttttctt aacaagtccc atttgttcta agagtaagat aagtttttct
     3121 tcttgctttt taaagtggaa gatctgagtc cgacttcgga ttgtcggtaa catcttttcc
     3181 tcatcgctag tcaagaagaa aatataaact tcactctggg gttcttcgat gaccttgagc
     3241 agagaattgg ctgcgttggg atgcatttta tccgcttgct cgatgataaa gacctgttgc
     3301 tggctttcaa tccctgcttg agaaaactgt cccaccaatt cccgaatacg ttctgtcttg
     3361 atgacctgat tgactggctt aatcaaggtg acatctggaa actcttcctg ttcaatcagc
     3421 ttgcaacttc ggcatttctc acatggtaag acgccaactt tatccgtaca aaagaggctc
     3481 ttagctaaaa attgcgccat ttccaagctt ccaaagaaac ctgaaaagag ataggcgtga
     3541 ttgagctggt cttgttctaa gatacggaca aaacggtcaa actgagctgg ttgccaagcc
     3601 tttagttgat cttgtttcat ttggccaagc ccattccgtc aaacaagaca gccttggtag
     3661 tttccacaac ttgctccaaa gggagactag catcaatctt gacaatgcga tttccctctt
     3721 tatccagaag agaaaggtag ccttgacgaa cttttttatg caagtccaac ccttccaaat
     3781 ccaaacgatt aacctcgcgg tcactattag cagcaatacg agccagccct tcttccacct
     3841 cgatgtcaaa atagagtgtc aaatcgggtt tgaggccatc tgtcgcaaac tgattgagcc
     3901 agtcaatggc ttcaatatct aagccacgac caaatccctg ataggcaaca gaactatcga
     3961 taaaacgatc catgatgacc aacttgccag cttcaagggc tggaagaact ttttccacca
     4021 aatgctgtct gcgactggca atatagagaa gtagctctgt tttagcatcc atctgagtat
     4081 gacttggatc caaaatcact tcccgaatct tctccccaat caagactccg ccaggttcac
     4141 gggtcgtcag cacctttact cctttttcct ctaaaattgg tagcagagcc tctaaaacac
     4201 tggtcttgcc tgctccctct ggtccctcaa gagagactaa aaatcctttt gacatgtcta
     4261 actcatttct tttttactac ttctattcta tcaaaaaaat aggggtttgt gacaatcttt
     4321 ttgtcttctc atttcatact caatgaaaat caaagagcaa actaggaagc tagccgcagg
     4381 ctgtacttga gtacggcaag gtgaagctga cgcagtttga atttgatttt cgaagagtat
     4441 cagccaccat aaaagaggca gataaaacta cctcttattt acctagttct tgtgttcgtt
     4501 tataggcttg atgaactgcc tccatgactg tgcctcgaaa agcatgcgct tctaggcttg
     4561 ctacgccagc aatagtcgaa ccgcctggac tacaaacttg atctttcaaa actccaggat
     4621 gttgctggct ttctaggacc aattgcccag ctcctaccac agtttgggct gccattttca
     4681 aggctgtttc tcgtggtaat cccgtctgaa cacctgcatc tgccaaggcc tcaataaaga
     4741 gatagacaaa ggctggtcca caacctgcaa gacctgtcgc tgcattgatt aagctttctc
     4801 ctaattcaac caagagacca gccttggcta aaagctgaca aaagatctca ctgtcctcag
     4861 ccctgcaatt aggagacaag gcataactaa tcactccttg cccgatagaa gcaggggtat
     4921 taggcatcat acgaataatt cggtgttggc ttgggataag acttgctagt ttttctaagg
     4981 tcaatccagc tgccatcgaa atcaaaagaa gactttctct tttttcaagg atggtctggt
     5041 attgagaaag cagttcagaa aactgagcag gcttaacacc tagaaaaatc acatctgctt
     5101 cttcgaatat ttcttcattg ttagaagtct gaccaccgaa gttagcgatg aaagcatcca
     5161 ccttgacttg actacgattg gcaaggagaa tctcatctga cggtctagcc tgcaagacag
     5221 acttggttaa actagcaccc atattcccca aaccgataaa tccaatcttc atctcttact
     5281 cccttatctg tccatcacca gtgaccacat acttgtagct agtcaattcc ttcaagccca
     5341 taggaccacg cgcgtgcaat ttctgagtag aaatccccat ttcacaacca agaccaaatt
     5401 gtcctccatc tgtgaaacga gttgaggcat taacatacac cgctgcagag tccacttgat
     5461 ctgtaaagta tgctgcagct tcagcatttt ccgtcacaat ggcatccgaa tgatgggtac
     5521 tgtgggattc aatatgcgca accgcttctt ctaaactgct cacaacctta acagctagaa
     5581 tatagtctaa aaactcggta tcaaagtctt gtgcttgagc agcttgacct gaaacaaact
     5641 ggcttgcttt gctatctagg cggaattgaa ttggttccaa cccagcttct tttcgatctg
     5701 caaccaacac ttgctccaag cgaggaagga agcttactgc tttgttttca tgaaccagca
     5761 gaacctccat ggcattacaa acagaaggac gactggtttt agcattgttg atgatagaca
     5821 gcgccttgtc ttcgtctgca tccttatcca catagacatg gacaatccca gttcctgtct
     5881 cgataacagg tacaatcgca ttctcaacca ctgcattgat caagccagct cctccacgag
     5941 gaatgagaag gtctagatag cccttggcct tcatcatagc ataactactt tcacggctag
     6001 tatcctccac cagttgaatc acatttggat gaatagtagt cgtctccaag cccttcttca
     6061 aggctgtgac aatggcatgg gttgtttgat aggcatcctt accactacga agaacaaccg
     6121 catttccact cttaagagtc aaagcagccg catcagacgt cacatttgga cggctttcat
     6181 agataatacc gatgacaccc atagctacac gtttttttgt gataaccaaa ccattttcaa
     6241 gctgacttgt ttctaaaact tcaccgattg gatctggtaa ggcaaccact tcacgaattc
     6301 ctcttgccat cgcttctata cgatctgcat ccaaataaag acgatccaac atcacatctg
     6361 agattttccc cttagccgct gccatatcga gggcattagc cgctaaaatt tcctcagtag
     6421 cagccactaa gtgatcagcc atggctagca aggcttggtt tttcacttct tcactagctg
     6481 tgttaatcga ttttttaaca gcctgtacct gttcaaattg ttcttgtcta ctcaccatag
     6541 tttacctcta aaattctgta aaaagtagtt ggatttcagg agtaatggaa atccagtcgt
     6601 cacggtaaat caagacaccc ttggcttttt gaaaacgcaa catatcctcc aaagcagatg
     6661 ctccaaattg cacgcgtcct tttccaagtg attttccact ttccttatca aatactgtca
     6721 cgatatcacc gtaagaaaag actccttctg cttcaacgat accagataag agaagactct
     6781 ttccatgttg agagagagct tccgcagccc ctttatcaac ccaaatagaa ccttgactct
     6841 gagcatagaa ggcaagccat tgtttctggg tacgaagccc cttctcttga gcaacaaagt
     6901 aagaaccatc ctcggtctcc tctgccgcct caaccatgga atctgatttc aaggatgagc
     6961 agatataaac aggaactcct gattccgtcg cgatagttgc agccttgatt ttggttaaca
     7021 tacccccagt tccgtttgac gaaccagctc caccagccat atcaataatc tcacgattga
     7081 tggtctcgat tctctccaag cgtttggctc ttggatctga attaggattt ccagtataga
     7141 gaccgtccac atctgtcaag aaaactaaaa ggtctgcttg gaccatcgcc gctacttgag
     7201 cacttagagt gtcattgtcc ccaaccttga gctcatcaat aacgacacta tcattctcat
     7261 tgatgatagg aattgcccca cggttgagca aaaccgacaa agcctgatgg gcatttttat
     7321 aacgacgctt atccacaaag tcatcttggg tcagcaagat ttgtgcagaa acgatttgac
     7381 gcaagagaag attggtcgta tattcttcca acaaaagtcc ctgccctacc gctgctgaag
     7441 cctgtttatc agcaatttta gtcggacgct ttttaaatcc taaggctcca aaaccagccg
     7501 caatggcacc tgaagacacc aaaatcaact catgaccagc ctcgtgcagc atagccaact
     7561 gctgggtaat atcctttacc ttactacgtg ataaacttcc atcctcattt gtcagagaag
     7621 aagtgcccac cttaaagaca atccgtttgt atttcatagt ctcactccga ttattcatat
     7681 ttatccatta taacatgaat aacaggaaat agaaaagagt tgataggaaa aagaaggccc
     7741 taaaaccttc ttttctacta ctattctgat tccgatttgt ttacactcgt ttcagagctt
     7801 tgtgaagttg aggatgtcaa atcttgacta gttgaagaat ttgtaacact ttcatgttga
     7861 ctcgtttctt tattttcaag atcctttttc actacttctt tggattcttt tatttctgaa
     7921 tgacttgtag ttttaattgt agcagatttc tcgatcggaa catccactaa ccaggcacca
     7981 cttgaatcca cagtatagcc ttctggtgtc ttaccattca caagtaattg accattttct
     8041 ttcaagaagt accatttatc cttgtcttta atccaaccta tagctcgtga accacctgtc
     8101 ttgtagaaat accaatcatt tgccttttta agccagcctt gcttcatagc tcctgattct
     8161 tctagatagt aatgatgtcc agcaacttct atctcacctg tcttcatgat accagtagaa
     8221 tccatatagt accaggcttc tttatctttt acccagcccg ttttcatttc ccctgatgga
     8281 gcaaagtaat accattgccc cttatattga agccaacctg tcttcataga gccatcaaca
     8341 tccaaataat accactgctc tttatcttga acccaaccaa tttccatctg attttctttg
     8401 ttaaaataat accagattcc atcaattttc ttccaatttt tagcagaagc accagagtct
     8461 gtcaaataga accaatgatt gttccatttt ttccattgat tctgtaacaa gatacccgtt
     8521 tggttaaagt aataccattc accttcgatt tcattccaac cgacagcata ctctcctgta
     8581 gaatcaggcg cttgatacca ccagttccca tatgcactct tatgccaacc agcttgaaaa
     8641 cttggaatcg gcttgtagga tgttgaaata ttgacaaaac cgtcttttcg aatatcaaaa
     8701 actgttgcat catagtcttt gctggctgcg ttgattctct caattcctcg ttctttgagc
     8761 caattaacat actcactatc aacaccattt ttccaaggta gactatccga agtttgaaca
     8821 atcaaactcg gactcaaatt tttaatgaaa tccttggtat ttgatttgtt ggtatcatgg
     8881 tgatgattaa acttcatcaa atcaactttt ccaatgagag gaccatactt gtcttctgct
     8941 ccatgaacat tatctaaatc gcccccaagg taaattttct tgccattgac tttcaccacg
     9001 ctaatcaagg aattggaatt gtcatcccaa attttcttta attcacccga tgaatcagtt
     9061 tcattttcat aattatagag ctgaatatcc atgtccccaa actgaaaatg agcatcccct
     9121 tgtgtgatat tttgaataac tgaaacacct ttttctgcag cagtctgtaa aaccttatca
     9181 tagccataca gattatccca tagacgttca gaattagtaa tacgactatc actatatttc
     9241 ttaagataga ctcggtcaac tggataggta gacagtaatt catcaacatt tccaatatga
     9301 tcactgtggg tatgggtcac caaaataaaa tcaagttttt ggacacccaa ttccttcaaa
     9361 cgacgaaaga cacggtctgt tagaacatgc ttataagacg tttcaattcc ttctctccat
     9421 ggataacgag aatcacttcc atctgggaaa tcataatctt ctcctgtatc caccatggca
     9481 aaatgtccat tgctttcaag aataatcgca tcactgccac cttcttgaac attgataaag
     9541 tggattttat ttcctgaact ttcttgagcc tgaacattcc cataccatga caaacctaaa
     9601 aaagcgccta caagtgctaa actagttaat ttctttttca ttcttacttt ctcaatctct
     9661 ccaaggtttc cttaaaaatc tctgggatat ctgctttaaa ttccaaggtc ttacctgttc
     9721 tcggatgagt aaaacctaaa gtcttggcat gaagaaattg tccatgtcct ttcaaagtct
     9781 tgcgaggacc atagacctca tcaccagcga ctggatggcc gatataagcc atgtggacac
     9841 ggatttgatg agtgcgccct gtctccagtt gcaactctac taagctataa tcgccaaagc
     9901 gttccaagac gtgaaaacgc gtcactgcag gcttcccttt agcagttaca gcctgtttct
     9961 tacggtcttt ttcactccgg ccaatcggcg cttcaattac accacgatca ttaggcagat
    10021 ttccatgaac aatcgcccaa tatttgcgga gagacttttt atctttgagt tcttgggcaa
    10081 gtactagatg cgcatcatcg tttttagcaa tcatgagaag acctgacgta tccttatcaa
    10141 tacggtgaac aatccctgga cgcagaaccc cattgatacc cgacaagtcc ttaatatgat
    10201 acatgagggc atttactagg gttccactgg tatgaccagc actcgggtgc acaaccattc
    10261 cctgaggttt gttaacgaca gccacatcct catcttggta gactatttct agcggaagat
    10321 cctcagccac atactctaat acctctggtt ctggcacatg gtaagtgacg acatcaccct
    10381 cttggactgt gtatttagct ttcttgactt gaccattgac caagacctgg cctgatttaa
    10441 tttgttcatt cgcgagacta cgtgataatt ctgacaaatc tgacaaagcc ttatccaaac
    10501 gcagaccacc agtttcaatt ttaatttcca tttatttcct cttttagcat tgcaatcaat
    10561 aaaataatca ctccaaccgt cagatagcta tctgccacat tgaaaattgc aaagttgata
    10621 aagtcaaggt ggaacatatc cacaacaaag ccctgactga ccctgtcaat aaagtttcca
    10681 agaccacccg cgattattag agtcaaaccc aagaacatcc agaatgagtc ctccatgtgt
    10741 ttatgtaaat accaaatggc acctatcacg acaaccagag taatgacagc gaataacagc
    10801 tgctgatctt gtaagataga aaaggctgca cctcgatttt gcaggtaggt caagctaacg
    10861 aaattgggaa tccaggagcg cacttcaccc agtggaatct gctggacgat ataggatttg
    10921 accaactgat ccagcccaat taaaagcagt acaatgactg ccactattgc tctttttttc
    10981 atgatttcct cttttgatca aaatattctt gcatgacttc tacgaagaga gtcccagctt
    11041 gactaagctc cacttcttca cgtttaacat agaccatgcg gttatctagg ttatccttga
    11101 gacgaataac tgtaatgcca ttaacactgt cactatctaa aaatccagaa cctgtcgcat
    11161 aggcgtccgt ccgctccaaa ataccattca aggtggcacg gtctgtcaca ttaaacatct
    11221 gtgagctagc gctggtatcg acaaagttct ctgaataata aaggtactcg tctttctctt
    11281 gagtgaaacg aaccgttggt aaatccgcta aatcctccat gactaattcc tctttctggg
    11341 ctaaaggatg accctcacag agataaatat gggtatggaa aggaatcaat tcgatgacct
    11401 ccagacctaa cttttcaacc cgttgcataa tccccttttt attttgattg ttgaggtaga
    11461 taatcccaat ctcactatgc ccttgcgcca cttcatctaa tatttgaaca gtagttgatt
    11521 caaaaatacg gaagttctta tagtcaggat agcgctctga aaaggccgta atagttggtg
    11581 gtaagaagtc atagtgctgg ctagcaacgg aaaattcatc tttttcttct tcaggattgg
    11641 catactgatt ttgaaaaata tcaaatcctt taaccaattc ttgcgctttt tcataaaatt
    11701 ccatcccacg acgggtcaag aaagtccctg agctggtccg acggaaaatc ttaaagccca
    11761 actctttttc caaatcacga acagaaatag acagactcgg ctgactaaca tacatctttt
    11821 cagcagcttc acgaaaagta ccactattgg caatagccac aacatagcgt aattgttgaa
    11881 tgttcatctt ctacccccaa cttctttatc ttttcattat accatatttt agaagttttc
    11941 caaaaaggaa aaaagaacat cctattcttc ttaactatct tcactatctg cctttctcac
    12001 gccaatctta ttttcaaaat caattcaaaa aaataagtgg ctacacacca cttcagtatc
    12061 ggaaagaaaa acgttgacta tttgtgaaaa aagaaaatgc cggaaaattc cgacattttt
    12121 ttagtaagct aacttcctga aaataggagt aaccaaacat cccagaaggc agtgatattg
    12181 atgagaatat gaactagtat tggatagtag agatttttag tcatgcgata caatagagct
    12241 aaaatcagac ctccactagc ataaatgaaa aaagcaagag gagttaaagc aaaattgata
    12301 tgaatataac cgaaaataat agcagaaagc aaaacatctc cgtaccaagg tgagtttttg
    12361 aaaaaggttg tcataagcac acctcgataa atcaattcct cagcaatagg ggcgatgaag
    12421 caaacgatga gcaagaaata agggaactcc tgtctcccca tcatttctac cgtttcattc
    12481 aaagaaatct gatttgaaga cagggatatg aaatacgaaa agaggaagtc agacatatat
    12541 gaaatgatgt agcccagtaa aaggtagatg aagtacctca gctgccactt gaaatgaaaa
    12601 attttctttc ctgcaaaaac cagtagatag atgaccgcta agaaaagaac tccgctctcc
    12661 atcaaaagca gaatctgaaa aaattcacga cttgctggta gatagggctt tgcgaggtga
    12721 ttgaaaactc tccaaaacca agttgatttg taaaaaatta aggacaatgc tagtaaaatt
    12781 tgaatagccc gttttttcat attaaattct ctgctttctc cacttatttc ttcatattac
    12841 taataaattt tacttaaaat ctcgcagcac ttcctttgca aagatgattg cctcctccaa
    12901 tatatcctaa atcataggtg cctctgggac aaaatccatg aaccggcaga gttaatactg
    12961 gttctatttc ccaatcgtac ctaccattag gatttttacc atatgaatct gctccacctt
    13021 gtatcagctc taattcttca tttgttagat ttatattttt tgtcattaaa atcatgatat
    13081 atttcctcca ttattcttca gattagttga gtaatctgat actcatacct acttacaaaa
    13141 aaactattat attaagttgg ttttttaatt tgtctagttg caacattgac aaacatagta
    13201 tagcatatct ttgcgaaata tcctcttcaa atcatgaatt gtcatcaaaa catcttaaac
    13261 tataaaatca attagtctca agctttctat caatttcttc tccaaaatat gctataataa
    13321 tagcaaaaga taaagaagga agacctatga ttaaactact agccttggat atggacggca
    13381 ccctcctcaa tgaagccaag gaaatcccac aagctcacat tactgctatt cacaaagcca
    13441 ttgaaaaagg tgtcaaactg gttctctgta cgggtcgccc ccttttcggt gtcctcccct
    13501 actacaaaaa actgggactc gacctccaga atgagtatgt gattgttaac aacggttgtt
    13561 caactcacca gactagcgac tggggcttgg ttgactggca agaacttagt ccagctgaca
    13621 tcgaatacct ctatgacctt gctgaaaaga gtgatgttca gttgacactt tttgacgagt
    13681 cacattattt tgttctcggt ggaaagccca atcaagttat tgaaaatgat gctaaactag
    13741 tattttcaga cctgactgaa atttctcttg aggaagcgac tagtggaaag ttccggatgt
    13801 tccaaggtat gtttttagga acaaaagaac aaacagacga ttttgagcag cgttttgctg
    13861 aagagctttg ccaacgattc agtggagttc gttcgcagcc tgtcatttat gaagcaatgc
    13921 cacttggaac gacaaaggct actgctcttt cacgactagc tgagattttg aagattgatt
    13981 cctcagagat tatggccatg ggcgatgcta ataacgatat cgaaatgctc cagtttgcag
    14041 ggcttgggat tgcaatggga aatgccagcg attatgtcaa atctcttgcg gatgccgtta
    14101 cctcaagcaa cgaagaagac ggcgttgcgc gtgctattga gaaatatatt ctataaaaaa
    14161 gaaaagcaaa tagacagaag ttactgctat ttgctttttt gctaatattt taaaatacgg
    14221 actaatcaat agagaagaat agcgaatcat aaaaatcgat ttactagatg ccatagtatc
    14281 ttatagttgc taataagaag ttagtctagc agaatgaatc tctcatttct cccacactaa
    14341 tctgtaattt gtctatacat ttctggtctt ctatctcgaa acaagcccca gtttaggcgt
    14401 tcacttgctc ccttgtctag gttataagta gctaacagaa cagcttcttc ttgtctttca
    14461 gctcgttcta gaatagctcc tgtttcatcc gtcataaagg aggaaccgta gaagtcaaga
    14521 ctggaactct gtccgccatt ttcctcacta ggagtaacct cctctaaacc ataacgattg
    14581 gctgcgatga ctggaacaat attcgctgct gcgtgccctt gcatagtacg ttgccagtga
    14641 ccacaactat ctgtatccaa aattggctct gaaccgatag ctgtaggata aaagagcaat
    14701 tcagcaccat tcaatgcaag acagcgcgct gtttcaggga accattgatc ccaacagata
    14761 ccgataccaa tcttagcata gcgagtattc cagaccttga aaccagtgtt accaggcgtg
    14821 aaatagaatt tttcttgata ataatggtca tctggtatat gggtctttcg ataaacgccc
    14881 agcacttccc catctgcatc aatgacggca atagagttat acaagacatt accatctttt
    14941 tcatagaaac tgattggtaa aacaacttgt agttccttag caagcaactt aaaatgctga
    15001 atggcagtat tttccgctac agattgggca tactggtagt agtcatactg acgttcctga
    15061 cagaaatagg gatgttcaaa caactcgggc aagagaataa tttgggctcc ttgctcagca
    15121 gcctgacgta ctaaacgctc tgcggtttgg atatttgttg ccacatcctt agcgcattgc
    15181 atctgaatgg ttgcaactct tacatttctc atctttttct cctattctgg aatttgttgg
    15241 gtgatacagt ggatattgcc accacctaag agaatatctc tggctggtat tccgacaact
    15301 ttacggtctg ggaaacactt gctgaggata tctaaggcca cttggtcgtt tacatcctca
    15361 aactgtggaa ccaagacagc cttgttggcg atataaaagt ttacgtagga agctgctagt
    15421 cgttcacctg cgtatcgctc ttcttctcct tcttcataga tgtagcctgg caaatcttct
    15481 tctgtcacaa cttgtcgaac tgcagggata ggcaatttat gaatggtgaa gtgacaacct
    15541 tttgcatctg tttcctgttc taagagttcg agatctgctt ttgacatggc atactgggga
    15601 tcattttcgt catctgtcca agccaaaaca acctcagcag gaccaacaaa ggcaacaaca
    15661 ttatcgacgt gttcattggt ttcatcctga taaataccat aaggaagcca aataactttt
    15721 tcagcaccaa gactttctaa taatgtgttt tcaatctcct ctttagtcaa gttaggattg
    15781 cgaccaggac taagcaagca actttcagtt acgagaatag ttccttgacc atcgctatgg
    15841 attgcgcctc cttccagtac aaaaggttta gcatcataga caggcctttc caaggcctca
    15901 gcaaaacgac tggctacttg gtcatcctct tcataatctt gataaagacc atcataggtg
    15961 cctccccaag cattaaaggc ccaatccacg gctaatttct tacctttatc attgacgaga
    16021 atggttgggc cagtatcacg cgcccaggca tcattggtgg gaatgtctaa ataaacaacc
    16081 ttgtctccaa gataggattg ggcttcagat agataggcct gctccaccaa aagatagact
    16141 ctttcccctt ctgctatggt ctcgatgatc tgagtaaatg ctcttttagc agcctttcct
    16201 tgaaaaggcc atgatcctgg tcgagtcggc catatcatga gggtaccatg atggggttcg
    16261 tactctgctg gcatgtgata gcctaatttt tttggactgt ccatcatgat aatctccctt
    16321 taaagtcttg atagccaaaa gctttgagta agctacagtc tccctgttcg tccatgagat
    16381 agagacttgg caatccaata ccattaaagg tattattttt gacaaaagaa taaatggcca
    16441 tgtcttgaaa ataaagtctg tctccgattt ggactggatt ttcaaaacta taatcaccaa
    16501 tcacatcgcc agtcagacag gtattagaag aaagtctgta ggtatgggct ttttcctgtg
    16561 actcaaagcc atttctcaaa ggtggacgat agggcatctc aagtacatca ggcatatggc
    16621 aggtcgcaga ggcgtctaaa accaagattt ccataccgtt ttctacaata tctaatacct
    16681 cagttgctaa ataacccgca ttaagcgcaa tggcttcacc aggctcgata tagatttcaa
    16741 gattgtaagt ttttcggata cgcttgattt ctgaaatcag caaatccaca tcgtaacctt
    16801 ctcttgtaat atgatgacca ccacccatat tgagccattt tacctcatgt aagtagggac
    16861 caaactgttc ttctactgct ttcaaagttg tttgtaaatc atctgctccc tgctcgcaaa
    16921 gggtatgaaa atgaagtccg tcaaccaaat ctagcaaatc gctcggaatc ttgtctatag
    16981 taactccaaa gcgagaacca ggtgcacaag ggtcatagag cgcgtgatct ccttgagttg
    17041 aacactgagg gttgaggcgc aaaccaacac tgacaccagc ctctcgacaa cgagccccat
    17101 gcttacgcaa ctgtctctct gagttaaaga cgatatggtc tgttatccca agcaattcct
    17161 ccatgtcctc atccttgaaa gctggtgcaa aaacatggac ttcaccagga aattcttccc
    17221 ttgccaattt ggcctcatag agtccactag ctgtcgtacc tgatagatac tggctaatca
    17281 agggataagt tttgtagagg gaatatgcct tctgggcaag caagaccttg caaccggcct
    17341 cttcttgtac atattgtaga atgcggcaat tagcttctaa cttggccaag tcaataacat
    17401 aggctggtgt tggtacttgt tctaacttca ttagtccaac atttgtggat tttcaaccac
    17461 aacccatggc aaaccatact cattcaaagc ttccatgaat ggatctggat ctaactcctc
    17521 aaggttatac actccagctt gtttccaagt tccgttcatg actaattttg tcccaatcat
    17581 ggctggaacg cctgtcgtgt atgaaattgc ttgtgacccg acttctgcat agcactcttg
    17641 atggtcgcaa acattgtaga tgtagatagt cttttcaacg ccatctttga cacctgtaaa
    17701 gatacatcca atattggttt tacctacagt acgtggacca aggctagcag gatctggaag
    17761 caaggctttc aaaaattgaa ttggaacaat ttcttggccg ttaaagttaa tggtatccgt
    17821 acgaaggagt ccaacatttt caagacattt catgtgcgtc aagtaagatt gaccaaaagt
    17881 cataaagaaa cgaatgcgtt tgacacctgg aatgttcttg gccaatgatt cgatttcttc
    17941 atggtgaagg agatacatat ctttttgtcc aacttgaggg aaatcatact cacgcttgat
    18001 agacatagct tcgacttcga cccatttccc atcttcccag taagaacctg gcgcagaaac
    18061 ctcacggaga ttaatttctg gattaaagtt ggttgcaaat ggataaccgt ggtcaccgcc
    18121 attacagtct aaaatgtcga tataatggat ttcatcaaaa tagtgtttga gggcataagc
    18181 tgaaaagaca ctagttacac ctgggtcaaa accagaacca agaagagcag tcaagcctgc
    18241 ttctttgaat ttctcttgat aagcccactg ccatgagtag tcaaagtagg ctgtaaaacc
    18301 aagttcctta caacgttttt cgtagatagc acgccactca gggtcttctg tgtcttctgc
    18361 ttcgtagttg gctgtatcga tatagtgaac acctgttgcc aaacaagcat ccataatggt
    18421 taaatcttga taaggcagag ctacattcaa aacagcttct ggtttgtagc tttcaatcag
    18481 agcaatcact tcttcaacct tgtcagcatc aagagcagct gtctcaatct ttgtacttgt
    18541 tttgccttcc agtttagctt tcaagtcatc gcattttgac ttggtacggc tagcaatcat
    18601 aatctctgta aatgtttcgc tatcttgaca aatctttgaa atagcaactt gggcaacgcc
    18661 cccacaacca ataactagta aacgactcat ttttttcctt cctcttcttc taaaatgtcc
    18721 tcaacatact tgggcaacat aaaggctccc acgtgtaagt ttgcagtgta gtattctgtg
    18781 aaaagctggc gttttttcca gccttccttg tcaaaatctt tgacagggtg gtattttttc
    18841 gatgcaaatc caaacaacca atagccagct gggctagttg gaatatgggc ctgataaacc
    18901 cgactgattg gaaaggcttg attgaccttg cggtgcatgc ttcggcaggc cgactcatcc
    18961 tcgtcaaaga acggactccc atgctggtaa atcatgatgc cgtcttcctt cagagctcga
    19021 taactattgc cgtagaattc cttggtaaag agtccttccg tatggccaaa tggatctgtc
    19081 gcatcgttga tgataatatc atagtcatat tcgcagtttc gcaaaaagcg tagcccattt
    19141 tggtagtaaa tggtaacacg aggatcatct aggcctgcag caaagtctgg gaaatactca
    19201 cgacagacct cgaccaacat ctcatccggt tccacgatat cgatttgttc cagttcagga
    19261 tagagtgtta atacttgggc aacaccgccg tcaccacccc caataaccaa tactttcttt
    19321 ggatttgggt ggacagccat gggaacgtga acggtcattt cattgtagac gaaatcatcc
    19381 gcatctgaga acaagacatg gccatttaaa atcagtattt tcccaaaagc tggcgtatcc
    19441 aagacttcga tatcctgcca ttcacttttt ccagcgtaaa gttgcttggc tgttctcaga
    19501 gacaatttga catctggagt atgaacttca gaaaaccata aatccatcta tttctcctta
    19561 tattata
//
LOCUS       DAVBCV010000041        18889 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBCV010000041 DAVBCV010000000
VERSION     DAVBCV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.041"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            36..464
                     /locus_tag="VQ516_001427"
     CDS             36..464
                     /locus_tag="VQ516_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2511854.1"
                     /translation="MSKSTSKRYYQVAIPFTRLFCLVLVSIDYINCLSETADKNWHKS
                     DRIFVTNTGKTVHSSILSKSLQRANERLKKPIPKHLSPHIFIHITISILSENKIPLKT
                     ITDRVGHPDSEVTNSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(956..1366)
                     /locus_tag="VQ516_001428"
     CDS             complement(956..1366)
                     /locus_tag="VQ516_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511855.1"
                     /translation="MPKKKIERISVIHREKILWLKWYFMRDKEKPKYSVLECKMFDAA
                     KNKDMLAYKKYATIKQITDIRVQTSEDDILTAIKEVYVYNHMNVIGACQRILFVSQSP
                     AYNKLNKWFETYSDLYFSIIPLPNMGAYHELVDS"
     gene            complement(1384..1761)
                     /locus_tag="VQ516_001429"
     CDS             complement(1384..1761)
                     /locus_tag="VQ516_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511856.1"
                     /translation="MRKYLKEIKELQELKELLSSRNTPEVIIVEGNDDLGEFFQVDGE
                     LFSDIELLENLKKWREWEVQVIVDDWCNRGLNEYETGILYFPKHEDKMDYIRFNKGLE
                     PLYHALDEPYTTISKSEWLKLLD"
     gene            complement(1703..2131)
                     /locus_tag="VQ516_001430"
     CDS             complement(1703..2131)
                     /locus_tag="VQ516_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET2511857.1"
                     /translation="MAGNENDGLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRLKQDEMMDKTTQMLYLASSNAVSVLNDIMMDAKINPFIRTQAAKTILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGDQRVTRTQGIIK"
     gene            complement(2175..2780)
                     /locus_tag="VQ516_001431"
     CDS             complement(2175..2780)
                     /locus_tag="VQ516_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511858.1"
                     /translation="MTFTTIKDDIKAFGKKKHGYMTGYIAKQEELHKQLQNGMIGEKY
                     AKDQLEAYKLEGDTYSRDTYNKIHAEIEKQHDLELEALKEKELSVTADDVAELTLLAS
                     MKMTKDELLGYFEKYKNKPLAIKKLWSIAEQYPEIAINLELFNAEQALESLILFFKRQ
                     LSYCHYSLLINGDKIQAVTTEMVVNSDAPELDRRLDEYLNK"
     gene            complement(2870..3043)
                     /locus_tag="VQ516_001432"
     CDS             complement(2870..3043)
                     /locus_tag="VQ516_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511859.1"
                     /translation="MTENKDNKLLEMMEKGFVLYSKNGIIKYIEIPDHGSIKLKAQDG
                     QLVYKEVTTGEQC"
     gene            complement(3642..4538)
                     /locus_tag="VQ516_001433"
     CDS             complement(3642..4538)
                     /locus_tag="VQ516_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2511860.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EVIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYLQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFTIKDKRYSINQLKNKVKQLK"
     gene            complement(4550..5359)
                     /locus_tag="VQ516_001434"
     CDS             complement(4550..5359)
                     /locus_tag="VQ516_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET2511861.1"
                     /translation="MAKTKVYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFNNLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRKKGKLEEVQPSLTFSNSCPTEIEKELEIEVDKEQSPTPST
                     INQDFVNLYKSFEAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYI
                     KAILNRWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(5434..5649)
                     /locus_tag="VQ516_001435"
     CDS             complement(5434..5649)
                     /locus_tag="VQ516_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511862.1"
                     /translation="MNELDISNTQAVFVTVILIGLLLYLNHRDRKKSAQLERENQQTI
                     ETPSEDLNPDYGRYIQLAGVDVWGRIE"
     gene            complement(5651..5851)
                     /locus_tag="VQ516_001436"
     CDS             complement(5651..5851)
                     /locus_tag="VQ516_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511863.1"
                     /translation="MTLDLDNMTQAEFDEAMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAEQVAYIKNYKARA"
     gene            complement(5848..6039)
                     /locus_tag="VQ516_001437"
                     /pseudo
     CDS             complement(5848..6039)
                     /locus_tag="VQ516_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841301.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6028..6318)
                     /locus_tag="VQ516_001438"
                     /pseudo
     CDS             complement(6028..6318)
                     /locus_tag="VQ516_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(6488..6640)
                     /locus_tag="VQ516_001439"
     CDS             complement(6488..6640)
                     /locus_tag="VQ516_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511864.1"
                     /translation="MKIVTFKPTKQIDGGFYLPGIDILFVSDKADAKSTEDVILFISR
                     NGLNKS"
     gene            complement(6651..6917)
                     /locus_tag="VQ516_001440"
     CDS             complement(6651..6917)
                     /locus_tag="VQ516_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511865.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLALKRYQTPMDGAKKVFYRLSDVYLFLAIK"
     gene            complement(7128..7310)
                     /locus_tag="VQ516_001441"
     CDS             complement(7128..7310)
                     /locus_tag="VQ516_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2511866.1"
                     /translation="MLITLSLAEKVRIKRARLQLTKKAVSEQLGIKSQTLTKVENGNY
                     DASKRIYESVMTWLVE"
     gene            7372..7875
                     /locus_tag="VQ516_001442"
     CDS             7372..7875
                     /locus_tag="VQ516_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2511867.1"
                     /translation="MYKMNRIKQLRKENNLTQRELANETKIPYRTIQRWENGETDIKS
                     DAAQVLADYFGVSIPHLLGYSDLIADTDSDIKAMAYSHLLTFVDEKQIKEFEKEFIRA
                     NDNSDTFGDLGSGEKALEKYYQALCYLVPPFRDILARYSFLSLDEKKSVYNIIVGLSN
                     NLSKENK"
     gene            7949..9112
                     /locus_tag="VQ516_001443"
     CDS             7949..9112
                     /locus_tag="VQ516_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2511868.1"
                     /translation="MNIIEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDTLNQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQASPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAISNL"
     gene            complement(9266..10570)
                     /gene="eno"
                     /locus_tag="VQ516_001444"
     CDS             complement(9266..10570)
                     /gene="eno"
                     /locus_tag="VQ516_001444"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2511869.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10734..11180
                     /locus_tag="VQ516_001445"
     CDS             10734..11180
                     /locus_tag="VQ516_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET2511870.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(11177..12292)
                     /locus_tag="VQ516_001446"
     CDS             complement(11177..12292)
                     /locus_tag="VQ516_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2511871.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIDVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGRHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(12301..12630)
                     /locus_tag="VQ516_001447"
     CDS             complement(12301..12630)
                     /locus_tag="VQ516_001447"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET2511872.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGGNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(12584..12946)
                     /locus_tag="VQ516_001448"
     CDS             complement(12584..12946)
                     /locus_tag="VQ516_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET2511873.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHC"
     gene            complement(13005..14438)
                     /gene="glgA"
                     /locus_tag="VQ516_001449"
     CDS             complement(13005..14438)
                     /gene="glgA"
                     /locus_tag="VQ516_001449"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2511874.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(14435..15574)
                     /gene="glgD"
                     /locus_tag="VQ516_001450"
     CDS             complement(14435..15574)
                     /gene="glgD"
                     /locus_tag="VQ516_001450"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2511875.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTFLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(15564..16706)
                     /locus_tag="VQ516_001451"
     CDS             complement(15564..16706)
                     /locus_tag="VQ516_001451"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2511876.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(16696..18624)
                     /gene="glgB"
                     /locus_tag="VQ516_001452"
     CDS             complement(16696..18624)
                     /gene="glgB"
                     /locus_tag="VQ516_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2511877.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         5613 a         3955 c         3179 g         6142 t
ORIGIN      
        1 acctgagtac aatcctattg agaaaacatg ggctcatatc aaaaagcacc tcaaaaaggt
       61 attaccaagt tgcaatacct tttacgaggc tcttttgtct tgttcttgtt tcaattgact
      121 atataaattg tctaagcgaa acagccgata agaattggca caaaagcgac cgtatttttg
      181 ttaccaatac aggaaaaaca gttcatagtt ctatcttgag caagtctctc cagcgagcaa
      241 acgaacgcct taaaaaacca attcccaaac atctgtcccc tcacatcttc atacacatca
      301 ctattagcat cttatcagaa aataaaattc ctttaaaaac aatcacggac agggttggtc
      361 atcccgactc tgaagtcact aattccatct acacccacgt cacaaagaac atgaaagatg
      421 aagcaatcaa tgtactggat aaagttatga aaaagatttt ttaaaaagtt ttgtcccttt
      481 tttgccctct aaatacaaaa atagcccttc ggataaaatc cgaggggcta gaaacgttgt
      541 taaatcaacg gccgaacttt tgaatttcat ggttcgggat aaaatagttc actgaactat
      601 tttatttttt aaggttatca taatatcaaa tagttcaatt aaatacgcta aattactaat
      661 atacttttta cctttttcat tctaaaatgt aaagtacaaa caattacaat atactagagg
      721 gggagtaaaa aaggtaataa aattgacgag ttcagcaggc aagggaaata gcacttttca
      781 tggtgttatt tttttaattc agcaatggag tagccgctta attgatagaa acaaaaagca
      841 cctagtttcc tagatgctag cacaatagcc cggattcgaa ccggaatctc ctcaattaag
      901 gtgcaatccc tatatactac tcattgtgct ttggttcatt atatcataca atcaactagc
      961 tatctaccaa ctcatgatac gctcccatat tcggtaatgg aataatacta aaatacaaat
     1021 ctgagtaagt ttcaaaccat ttattcagct tgttataggc tggcgactga ctaacaaata
     1081 atatccgttg acaagctccg ataacattca tgtgattata cacatagacc tctttaatgg
     1141 ccgttaaaat atcgtcttca cttgtctgta ccctaatatc tgttatctgc ttaatcgtag
     1201 cgtatttttt ataagctagc atatctttat ttttagcagc atcaaacatt ttacactcaa
     1261 gaacactata cttaggtttt tctttatctc tcatgaaata ccacttgagc cataaaattt
     1321 tttctctgtg aattactgaa atacgttcga tttttttctt tggcatgatt acctcctaaa
     1381 ttattaatct aatagcttta accactcact ttttgagatt gttgtataag gttcatctag
     1441 cgcgtgatat aaaggttcta aacctttgtt aaatcggata tagtccattt tatcctcatg
     1501 ctttggaaaa tataagattc ctgtttcata ttcatttaga ccacggttac accaatcatc
     1561 aacgataacc tgcacttccc attcacgcca ctttttaagg ttttctaaaa gttcaatatc
     1621 actaaataac tcaccatcaa cttggaaaaa ttctcccaga tcatcgttac cctcgacgat
     1681 gataacttca ggcgtatttc tactacttaa taattccttg agttcttgta actctttgat
     1741 ctcctttaga tacttcctca atttctaacc tcaattcttc aatttgtctt actactccaa
     1801 aaatttcatg agttttatat gattgttcaa gtatagtttt tgctgcttga gttcttataa
     1861 aagggttaat tttggcatcc atcataatat cattgagtac agaaacagca ttggatgagg
     1921 ctagatatag catctgtgta gttttatcca tcatctcatc ttgttttagt ctcaatgtct
     1981 ttttaacagc tgcaactttt agatacttat gccctgttgc ccttgacacc cctgcttttt
     2041 ggcacgcttt atctatcgtt ggctcagtaa gcatggcatc tatgaatttt atttgtttag
     2101 atgtaaggcc atcattttca tttcctgcca tctattacct cctcgttatc aaaataaagg
     2161 ggttgctagc ccctctattt gtttaaatac tcatccaaac gacggtcaag ctcaggggca
     2221 tctgaattta ctaccatttc agttgttaca gcttggattt tatcgccgtt gataagcaaa
     2281 ctgtaatgac aatatgaaag ttgccgctta aagaataaga ttagactttc tagcgcctgc
     2341 tctgcattga ataattccaa gttgatagca atttctggat attgttcagc aattgaccaa
     2401 agttttttaa tagcaagagg tttgttcttg tatttttcaa aataacctag caattcatcc
     2461 tttgtcatct tcatactagc taacaaggtc agctctgcca cgtcatcagc tgttacgctg
     2521 agttcttttt cttttaatgc ctctagctct agatcatgct gtttttcaat ttctgcatga
     2581 attttattat aggtgtccct agaataagtg tccccctcta acttataagc ctctagctgg
     2641 tctttagcgt atttttcgcc tatcatacca ttttgtaact gtttatgtaa ttcctcttgt
     2701 ttagcaatgt agccagtcat gtacccatgc tttttcttgc caaaagcctt aatatcatcc
     2761 ttgattgttg taaatgtcat gttatcctcc ttacgaaaaa ataaaagagg tatgacaaaa
     2821 gagcgttaaa ctcaattatc atacctctgg tttttccagt cagtatttat taacattgtt
     2881 ctccagtggt cacttcctta taaactaact gcccatcttg agcttttagc ttaatgctac
     2941 catgatctgg aatttcaatg tactttatta taccattttt tgagtataaa acaaagcctt
     3001 tctccatcat ttctaataat ttattatcct tgttttcagt catgaatttc tcctttattt
     3061 caagtgtccc aacgggtacg gttgtaagct gagacatttt agggctatct ttgagcgaca
     3121 aaaatgtcga aaagtcttca gttcctgtat aaccgttatt agttttgttt gctactcatt
     3181 tttgagtcac aaatctgtta agcaaaaatg agtaatagct cgtaagataa gtaaatctaa
     3241 tacttgtatt cctaaagcgt tgatataact gatattttca actatccaac gctctttact
     3301 acccttttta gtgtgcttta gctcattttt gagctttttt gagctttttt gagttccatt
     3361 ttgggaatgc aaagcaggcg cgcaccgtgc atgctttttt cttgctcata gcctcaccct
     3421 cagaagtttg ccgaccgaga gcgtggggct ttttattttt gtataattaa aaacagctgc
     3481 acaatttttg tacagctacg accgaggcag gggcgacctg ccgtacttta cactagataa
     3541 tatcatctag gtagtccaac tgtattttat catattaaag gctacattac aatttctcac
     3601 cctgtgaaga gtgtctattt ttgtttaggt ttgtaagtcg attacttcaa ctgctttacc
     3661 ttatttttta actgattaat tgagtatcgc ttatctttga ttgtgaaagc cttaaaagtg
     3721 ttaccctcaa gaccttttag aatacggcta taattccgct cgttgtatac tatcttgagt
     3781 tcatcgctat caagatttgt attaaatata gtattttctc gattattcag tacatcaaaa
     3841 agtaaatcct gctcccaatc gctcttaggt ttaataacag cattttttgc acctaaatca
     3901 tcaataatta agtaatcaac agacttcatg agttcagtag cttcaaactc tgtaagtgtt
     3961 gcacctttac cataattcca cccctcttta atttgtttga tcatttcggt taggcttaca
     4021 aaaagcacac tcttaggttc tccttttgtc ttatacccct catttatacc tttggcaata
     4081 gcaactgata aaagtgtttt tccaatccct gtacctcctg tgataagcgt atttcccctc
     4141 atgccatcaa gatatttttg tacctgacct tttgcaaatt ctaaaaatcg cttttcttct
     4201 gatgttacag cattaaaatc atcaaaagtt ttagttttaa actcatctgc tacatagctc
     4261 ttattgctca tcaacacatt ataagtttgc agatatagtt tagcattcaa attatcagca
     4321 atcgcatctt cttcatcttg ctttttctgt tcttcttggc attgttcaca atagggtggg
     4381 atacagcgaa cttcttttat tacctctccg ttctcattcc accccactac tacatgtctt
     4441 tctcctttga tttgtgttag ctgtatttca tgcttaggac acaattcgtc tagtttaaat
     4501 gtctcaatat ttcctaaact agattgtaat gatttcattt tctgacctcc taaaatggtt
     4561 tttcttgtgt tggtatccaa tcttcatagc tggtaggctc tagttgattg gtttgctgtt
     4621 ttttagcctc acgcgctgcc ctgctatttc taacaagttc caccgtcaat aaattgtcct
     4681 gtttccaacg gttaaggatt gccttgatgt atgcaaagtt tgctttaccc tgactgacag
     4741 cctcttttaa cgcctcatgg ataagctctg ggctaaaatc ttctagcata tactgcaatt
     4801 cttgaatctg taacggtgac aatgctttac ctgtctcagc ttcaaaagat ttatagagat
     4861 ttacaaaatc ttgattgatg gtagagggag tgggtgattg ttctttatct acttctatct
     4921 ctaattcttt ctctatctct gttggacatg agttggaaaa tgtcaaagat ggttggactt
     4981 cttccaattt tccttttttt cgttgttcac gtttataact cgcccagttt gtttcactca
     5041 taaccatagc ttttgcctgt ggtagttctg cgtttttatc ttcgtcaatc tgtattaaac
     5101 cgcattttgt aaagtatgcc atggtcatat ctatatcatc ttctgataca tctaatttta
     5161 gggctaactc ttccttgaga ttgttaaagt agccctcata gtataaaata cagtcacttt
     5221 ccaagctctc taacatgagc ctaatataaa taactgtcat agtgtaccca ccaggtaccg
     5281 tctttagtct cttgataaaa atattatcaa aaaatttctt atcaattttt agccaaaaat
     5341 agacttttgt ttttgccatc gtcctctccc ttgctcattt ataattctct accttgatta
     5401 gtcttaatac tcgcttgtca ttcattgtta gactcattca attcttcccc atacgtctac
     5461 ccctgcaagc tggatatatc gcccataatc agggtttaaa tcctcgctag gtgtttctat
     5521 cgtctgttgg ttttctcgct ccagttgggc gctttttttg cggtctcggt ggtttaaata
     5581 aagcagtaag ccaatcaaaa tcacggtaac aaaaaccgcc tgtgtattgc ttatatctag
     5641 ttcattcatg ttatgccctc gctttgtaat tcttgatgta tgccacttgt tcagctcgct
     5701 tcatattcaa caagtcgtct atttccacac tggttacttt tttatttaca aaatcagaaa
     5761 tcagttgaaa aatgtttgga ctttgtagct ggatttcagc cattgcctca tcaaattctg
     5821 cttgtgtcat gttgtctaaa tctagtgtca ttgcattgcc tcctcaaact tctctataag
     5881 acaactttta tttgctttct gagttccatt tttagagtta aaaagaatat attttaaggt
     5941 tacagtagcc tctaaatact ccttttcagc atgctctata tacgcctgtt gctctgcttc
     6001 gttctcaaaa aagtgcttag cttagcgttt aaagaatgct ttgactaggt ttagtttgaa
     6061 ttgccgtact gcttcggtat tctttagata agtgatcagc aaagtagcct gttgctcgtt
     6121 cagaatatag gattttttag gttgtcctct agtgtctaat ttatggattt taaatccaag
     6181 tattcccaac tcttcaaagt caaccttatt ttctcttatt aagcgtgtga tagtgtggtg
     6241 ttgtacgtca gcacattcag cgatgatctc gcttgtggta tacggctctt tcttgccgtc
     6301 catgtagact agttccatta tggttctacc tcctgtataa atttagctag cttacttttt
     6361 aattgtctcc tctagccctt tttagcctct acaacggttt tggctaacgt gttagaggct
     6421 tttataacgg cctgtatagc tgtctctatt tcctccgtgg aatagtagct ttgttttcta
     6481 atatcattta agacttgttt aacccatttc ttgaaataaa taaaattaca tcttctgtac
     6541 tcttggcatc tgctttatct gagacaaata gaatgtcaat gcctggtaga taaaacccac
     6601 cgtctatttg tttagttggt ttgaaagtaa ctattttcat ttcataactc ctattttatt
     6661 gctaaaaata aatacacatc tgacagacga tagaatactt tcttagctcc atccatcggg
     6721 gtctgatatc gtttaagagc aagatcctcc cagttatcaa gggttgtagc acttatacct
     6781 agcagatcca ttaattcttt acgagaaatt aaatctaact tactagatcg ttgtttttct
     6841 agctcaacta aagtttcaag agtctgttta agaagctgta aaccctgtaa ctgtatttct
     6901 ttagacagaa tttccattta tatcctccat tttccttttt ataaattaag ttgtcttttt
     6961 cctctaattt tgataaaata agaagagatt atttagcaaa gggggttgtc cctttgctaa
     7021 atacctaaca aaaccacgcg ccttgccttt gcttgagttt tgttattttt ccttagccgt
     7081 gtacgttggt ttggtcgcct gtacatggct ttttacttgc gttaaattta ttctactagc
     7141 caagtcatca cgctctcata gatacgctta gaggcatcat agtttccatt ctcaactttt
     7201 gttagtgttt gagatttaat acccaactgc tccgatactg cttttttggt tagctgtaat
     7261 cttgcacgct tgatacgtac tttttcagca agtgatagtg taatcaacat tggtttcacc
     7321 tccctttttt ctccattttt ggcattttgt gttataatct ctttagaaag gatgtataag
     7381 atgaatagaa ttaagcaatt acgaaaagaa aataatctta ctcaaagaga acttgctaat
     7441 gagactaaaa tcccttatag aaccatacaa cgttgggaga atggggaaac tgatataaaa
     7501 tccgatgctg ctcaggtact tgctgactat tttggagtat caattcctca cttactaggt
     7561 tatagcgatt tgatagcaga tacagatagt gatattaaag caatggcata ctcacatcta
     7621 ctaacttttg tagatgaaaa acaaatcaaa gaatttgaaa aagagttcat tcgtgcaaac
     7681 gataatagtg atacttttgg tgatttagga agtggtgaaa aggcgttaga aaaatactat
     7741 caagcattat gctatttagt tcctcctttt agggatatat tggctaggta tagctttttg
     7801 tcactagatg aaaaaaaatc agtctataat attattgttg gtttaagtaa caacttatca
     7861 aaagaaaata aataacttta aacatcatgt ataaatacct aacaaaacca cgcgccttgc
     7921 ctgctgatgg aaagaaaggt acaaatacat gaatatcata gagaaaatta aaaagaatgg
     7981 ccaaagagtt tattatgcta gtgtttatct aggcgttgac caactaacgg gcaaaaaagc
     8041 ccgtacaact gttacagcaa ccactaaaaa gggcgttaaa gtaaaagcgc gtgatgcgat
     8101 caatactttt gctgctaatg gctatacagt taaagataag ccgacaatta caacatacaa
     8161 tgagcttgta aaagtttggt gggatagtta caagaataca gttaagccaa atactcgcca
     8221 atccatggat ggattggtta gagtgcattt attgcctgta tttggcgatt acaagctatc
     8281 taaacttact acgcctattc ttcaacagca agtaaacaaa tgggctgaca aggcaaataa
     8341 aggcgaaaaa ggggcatttg ctaactactc cttgctccat aacatgaata agcgtatttt
     8401 gaaatatggc gtagctatcc aggtaataca atacaaccca gctaatgatg tcatcgttcc
     8461 acgcaaacag cagaaagaaa aggctgctgt caaatactta gacaacaaag aattaaaaca
     8521 gtttcttgat tatttagaca ctctgaatca atcaaattat gagaacttat ttgatgttgt
     8581 cctgtataag actttattgg ccactggttg ccgtattagt gaggctctag ctcttgaatg
     8641 gtctgatatt gacctagaaa gcggtgttat cagcatcaat aagacactaa accgctatca
     8701 ggaaataaat tcacctaaat caagcgctgg ttatcgtgat ataccaatag acaaagccac
     8761 attactttta ctgaaacaat acaaaaaccg tcaacaaatt cagtcttgga aattaggccg
     8821 atctgaaaca gttgtattct ctgtatttac ggagaaatat gcttatgctt gtaacttacg
     8881 caaacgccta aataagcatt ttgatgctgc tggagtaact aacgtatcat ttcatggttt
     8941 ccgccataca catactacta tgatgctcta tgctcaggct agcccgaaag atgttcagta
     9001 tagattaggc cactctaatt taatgatcac tgaaaatact tactggcata ctaaccaaga
     9061 gaatgcaaaa aaagccgtct caaattatga aacagctatc agcaatttat aaaaaataag
     9121 ggtgacccat ttccgggcta ccctcttact ataccaaaaa ttagtagggg tagtaaaaag
     9181 ggtattaaat tataaaaagc actaagggaa agcgccccaa agtgcttatt tcaaaggctt
     9241 tatagcctat aatcacataa ggagattatt ttttaaggtt gtagaatgat ttcaatccac
     9301 gatattcagc tacttcacca agttggtctt cgatacgaag caattggttg tatttagcga
     9361 tgcggtctgt acgtgaaagt gaaccagtct tgatttgtcc tgcgttagtt gcaactgcaa
     9421 tatcagcgat tgttgaatct tcagtttcac ctgaacggtg tgatacaaca gcagtgtaac
     9481 cagcttcttt agccatttcg atagcttcaa aagtttcagt aagagtaccg atttggttaa
     9541 ctttgataag gattgagtta gcagcacctt cttggatacc acgtgcaagg tagtcagtgt
     9601 ttgttacgaa gaagtcgtca ccaacaagtt gtactttctt accaagacgt tcagtaagag
     9661 ctttccaacc atcccagtcg ttttcatcca taccatcttc aatagtgatg attgggtatt
     9721 tgttaaccaa ttcttcaagg tagtcgattt gttctgcaga tgtacgaaca gcagcacctt
     9781 caccttcaaa tttagtgtag tcgtaaactt tacgttcttt atcgtagaat tctgatgaag
     9841 cacagtcaaa tccgataaat acgtctttac ctggtacata tccagcagct tcaatcgcag
     9901 caaggatagt ttcaacacca tcttcagttc cttcgaaacg aggagcgaat ccaccttcgt
     9961 cacctacggc agtttccaaa ccacgtgatt taaggatttt cttaagagcg tggaagattt
    10021 cagcaccgta acgaagggct tctttaaatg ttggcgcacc aactggcaag atcatgaact
    10081 cttggaaagc gattggagcg tcagagtgag aaccaccgtt gatgatgttc atcattggag
    10141 ttggaagaac tttagtgttg aatccaccaa ggtagctgta aagtgggatt tcaaggtagt
    10201 cagcagcagc acgagctaca gcgatagaca caccgaggat tgcattcgca cccaatttac
    10261 ctttgttagg agtaccgtca agtgcgatca tagcacggtc aatagcttgt tgatcacgta
    10321 catcgtagcc aatgatagct tcagcaatga tgttgtttac gttgtcaaca gctttttgtg
    10381 taccaagacc accgtaacga gatttgtcac cgtcgcgaag ttcaactgct tcgtgttcac
    10441 cagtagaagc tcctgatgga accataccac gtccgaaagc acctgattca gtgtaaactt
    10501 ctacttcaag tgttgggtta ccgcgtgagt ctaggacttc gcgagcgtaa acatcagtaa
    10561 taattgacat tttttactct ccttatgagt taaatttttt acacctctat aataccttaa
    10621 aacccctcct ttttcaagaa aaaacgttat ctttgtgcaa cttttcctta actttataaa
    10681 gtaatcgctt tcttttgtct gttttattct aacttttatg atatactgtt ttcatgacag
    10741 atttatcaaa acaattactt gaaaaagctc atggtgggtt aaaaataaat ccggatgagc
    10801 aaagacgcta tcttggtact tttgaggaaa gagttcttgg atatgtagat attgacacag
    10861 caaatagccc tcagttagaa aaaggctttt tatttatttt agaaaacctt caggaaaaag
    10921 cagagccact atttgtgaag atttcaccaa ctatcgaatt tgataagcaa gttttctact
    10981 taaaagaagc aaaagaaact gatagtcaag ccaccatagt atctgaagag catattactt
    11041 ctccttttgg cctggttatt catagcaatg caccagttca agtagaagaa aaagaccttc
    11101 gacttgcttt tccaaaactt tgggaagtta aaaaggaaga accagccaaa acatccttat
    11161 ggaagaaatg gtttagctaa atcttgcaca tatttaataa gtgcccaata ttggcagccg
    11221 tgcgctccag atagagactg gcatttttca aactatcttc taaaggttca cttttctcca
    11281 aaatagaaaa gacagcttgg atattttcaa atggtagggg aggtaaatct tcagcaagac
    11341 taccgcaaat agcaataaca ggaactccaa caggggttct ttttgcaaca cctataggcg
    11401 ctttcccagc aaagctttga ctatcaagtc ttccttctcc aacaacaacc aagtcagcat
    11461 ctgaaacttt cttatcaaag ttgattaagt ccaagcaggt atcaattcca gacacgatac
    11521 ttgcctgagc aaaagcacac aaaccaccag caaggcctcc acctgctcct gctcctttaa
    11581 tttctaatgt tgcaggtgag aatttttcat aaaaatcttg gatcgcctga tctacgactg
    11641 caaacatagt cggatgtcga cctttttgat tgccaaaagt ataagtcgca ccttgatgac
    11701 cacataaggg acttacgaca tctgctaaaa tatgaatttg aacaccttca ggaattttat
    11761 agcaattttc tgttgaaaca gaagctaagt ttaataagga ttgaccggaa gcaggcaaga
    11821 catttccatc cctatcataa aattgataac ctaaaccagc agcaatcccc agtcctccat
    11881 cattactggc cgtgccacca acatcgatat aaatatcttt aatcccttta gaaatgagat
    11941 ggagaatcaa ctctccaata ccacaagttt ggatttgaag tggatttcgt ttctctagcg
    12001 gaatttttcc aagaccaacc aagtcagcta cttcaaatag tgccagttcc cctttttgaa
    12061 aatagcgcat ggcttctttt tgtccaaaag ggtctgtcac ttggatccat ttttctttta
    12121 ggtcaagaga atgtcggata gcatctacag taccttctcc cccatcacca acagggcaga
    12181 ggagacattc tacatctgct atcgattgtt ggaagcctct ttttattgct tcagctacct
    12241 gttgagctgt caagctttcc ttaaacgaat ccggtgcaat tacaatcttc atattttccc
    12301 tcattctaaa cagtcaatca aaggaagaac ttctaaaaaa tccctcttgt caacatgatg
    12361 tggtatttct tttttgagca tttctttgga acaaaaggcg attcctaact ccgccgactt
    12421 caacattaat agattattgc ccccatcacc gattgccacc gttctttctt tagaaagttt
    12481 tagtttcttt ctccattttt ccagagtttc ttttttgacc tggggactta taatttgtcc
    12541 aactaatttt cctgttaaaa gaccttcttt gacttcaagc tggttagcag tgaaataggc
    12601 aataccaagg gattttgcta atctctccaa ctattggtgt aaatccacca gacaccagac
    12661 caactaggat accattcttt tggagaatag agatgaactc tgggacattt agcgatagat
    12721 gaattgagtt gaagacgtta tcaaagacca aaataggaag accttccaac aaggacactc
    12781 tttttcttaa actgctttca aagaccaact ctcctcgcat tgctcgactt gtaatctgcg
    12841 aaatttctgc ctcatgacct gcctctctcc ctaaaagatc aatcacttct tctaggatta
    12901 aggttccatc tacatccaaa acacacaagc cttttacttg agacatcagt tctcctctct
    12961 aaacagccta aaaatcgtat gaagtcatca tacgatttta tctattaatt aactaaacta
    13021 tggtacaagt caaggtatga cttgcaggct gtatcccatg agaagtcact ctccatagct
    13081 tgtttttgta ggtttctcca aatgtctgga tggtttctat acaagtccaa tgctgtttgg
    13141 aaagtccaat ttaaccaata aggagataga ttgtcaaagc taaagccagt accgcttcct
    13201 tcgattggat tgaaagcgcg aactgtatct cgcaagcctc caacttcatg gaccaatggc
    13261 aaggttccat aacgcatagc catcatttga gacaagccac acggttcaaa acgacttggc
    13321 atgaggaaga ggtcacaagc agcgtagatt tcctgagcaa gtttgacatc aaaagtgata
    13381 tttgttgata gcttgtctgg gtaaatctga gcaaaccatg agaaagctcc ttcaaaggct
    13441 ggatcgccag ttcccaaaag aacaatctga acatcttctt gcaagatatg gtgaagactt
    13501 tcgaccacca catcaaaacc tttttgacgt gtcaaacgag aaacaattcc caccagtgga
    13561 acgtctgctc taacaggcaa gccaactctt tcttgcaatt ttgccttatt tttggctttc
    13621 ccagacaaat cttcctgatt gaaatgatag tctaaaagag catccgtctg aggattataa
    13681 agatcagcat caatcccatt cacgatacca gatactttac cagactccat tttaagaatc
    13741 tgatccaaat tacatccaaa ctgactagtc ataatttcat gagcatagct aggtgaaacg
    13801 gttgaaacac ggttcgcata gagaatacct gccttcatcc agttcagaca gttgttccat
    13861 cgaagggtgc catcagcgta acgttcaaag ccaactccaa acaaatcacc caacattcct
    13921 tctgaaaatt gtccttggaa ttctaaatta tgaatggtta aaactgtttc aatgtcctca
    13981 taggcttgaa tccaacggta tttttccttc aacaagaaag gaatcatagc tgtatggtag
    14041 tcatgaacat ggagaagatc aggaataaag tcaatccttt ccatagcctc aatggcagcc
    14101 agttggaaaa aggcaaagcg ttctccgtca tcaaaatcac cgtaaacatg accacggaag
    14161 aaataatatt gattgtcaat aaagtagaag gttacaccat ttaatactgt tttcttaatt
    14221 ccacaatact gtctgcgcca accaacgctc acctcaaaat gaagcacatc ttcaatctga
    14281 tttccaaatt tagcctctac catatcatag tagggtaaaa tcactgcaac ttcgtgccca
    14341 gcttttacca gtgattttgg aagagcgcca atgacgtctc ccaaaccacc tgtttttgaa
    14401 aagggtgcac cctctgctgc tacaaataaa attttcatga atgaatatcc tctgttactt
    14461 tagcaccttt cttaaccaca actggatgtt ctgcagttcc tcgaatcaca acaccaggct
    14521 caacttcaac ccctttgtcc aagatagcat attcgacctg agccccttct ccaataacaa
    14581 cacgagggaa gagcaggcta tctttaacca agctatcctt atggacatgg atattacgtg
    14641 atagaacaga attagctact tgaccttcaa taatactacc agaggcaaac tgagaagttc
    14701 ttaccttaga tgtattagca tagtaagttg gctcttcgtt tttgaccttt gtataaatct
    14761 tttggtttgg tgagaaaaga gaatagaatt tttgtgattc aagcatatcg atattcgctt
    14821 gataataaga tttaacagag tgaatattgg ctagatagcc cgtgtactcg taggcgaaag
    14881 ctccctcttt tacagccaaa tcccgtaaaa catagcgcaa tttctctgga tgttcttttt
    14941 tagcttcttc ttccaagtgt tcaatcaacc aaggtgtatc aacgacaaag atatctgtag
    15001 acatattgaa cgtttcagct gttgacttgc tatcaaagag tttatgagaa agaacatggt
    15061 ctgtttcatc tacatccaag attgcattta cttctgaaat atctttctta gctagttttt
    15121 tataaactac agtgataggc tcttttgttg tactatgtag gtggaaaact tggttcaaat
    15181 caatgttaat aagaacatcg cagttgaggg caaccgtttg gtttgagcca gaacgtttca
    15241 aataagtaag aagctgttgg tagtattctt ttccaactgt actactttct acacgggtat
    15301 tgtaaattcc tagatagtaa tggctaagaa aggttgataa gccccactcg cgtcctgaac
    15361 gaatatggtc aaatactgag ctgatattat cctgctggaa aataccaaag acactacgaa
    15421 cacctgcatt agcaaggctt gaaagtggga agtcaatcaa acgatatttc ccaccaaatg
    15481 gcaaacttgc tactggacgg tggtccgtca atgtcgacat attgtgaaaa ccaactgtat
    15541 ttcctaaaat ggcagaatat ttatcaatct tcatctgttg ctacccccac tacttcatta
    15601 tatcctacaa cttgtacttc atctgttcca tcaatttcga caccgtcaga aataatcgca
    15661 ccttcaccaa taatggcacg tttaatctta gctccttgac caatgatagc tccactcatg
    15721 ataactgaat caaggacttc cgctccttcg cgaacttgcg cgcctgttga aaggatagaa
    15781 tgtttaacag ttccatcaac gaaacatccg tctacaacta atgagtcttc cacatgagca
    15841 tttgccccga ggaagtttgg tggtgaaatc aagtttcttg agtaaatctt ccattgacgg
    15901 ttacgactat ccaaggcatt ttctggagaa atatactcca tgttcgcttc ccaaagtgac
    15961 tcaatagtac caacatcttt ccaataacca ctaaattcgt aagcataaac actttcacct
    16021 gactcaaggt aatttggaat gacattttta ccaaagtctg acatgccaac cttgctcttt
    16081 tcagcagcga ctaacatatt acgaaggcgt tgccaatcaa aaatgtagat tcccatagaa
    16141 gcttttgtag atttaggttg agctggtttt tcttcaaatt caacaatacg attgttagca
    16201 tctgtgttca tgataccaaa acggcttgct tctttaagag ggacgtctaa aactgctact
    16261 gtcaagctgg cattattatc cttatgagac tggagcatat catcatagtc cattttgtag
    16321 atgtgatccc cagacaaaat caagacatac tcaggattga cactgtcgat atagtcgata
    16381 ttttggtaaa tagcgtgact agtcccctca aaccaacgat ttccttcact tgcagaataa
    16441 ggttgaagaa tagagacacc tgaattaata ccgtctagtc cccagcttga accattccca
    16501 atatggttgt tgagagcaag tggttgatac tgtgtaacga ccccaacatt atgaatccct
    16561 gagttggcac agtttgatag ggcaaagtca atgatacggt agcgcccacc aaattgcaca
    16621 gctggttttg cgatgctttg agtgagttta ccgagacgag ttccttgccc accagcaaga
    16681 atcaaagcta acatttcatt tttcattttc tactcctttt tggtttttat ttgtgacggt
    16741 tttagtagat ttcaagcgac gtttgatttt ccatacactt gctcccatag ccggtagggt
    16801 aaaggttaag gtctgctcat aatctttcca tagtccttct tgcgtttgaa cagtttgatt
    16861 atgttctttc caaacgcctc cccactcttc caactcagta ttccatactt cttcgtaaat
    16921 tcctgcaacg ggtagtccga ttgtaaaatc tttccgctca acaggtacca tgttaaagat
    16981 acagactaac atttctccct ttttaccctt acgaataaag gaaagaacac tctggtctcg
    17041 attatccgca tcaatgactt caataccatc atagctggta tcgatttccc acagacagcg
    17101 atgatctttg taaaactggt ttagctgaga agtgaaatac ttcatcttag cattcattgg
    17161 gtcttctagg ttagaccatt ccaactgttc ttcagatttc cattctagga attgaccgta
    17221 ttcgctaccc atgaagagca atttcttacc agggtgacaa atttggtacg tatagagatt
    17281 gcgcaagcct gcgaattgat tgtaacgatc tccccacatc ttatgcatca tactcttctt
    17341 gccatgaacc acttcatcgt gcgagaatgg caagagataa ttctccttga aaacatacat
    17401 aaagctgaaa gtcaccaggt taaagtcata tttacgataa atcggatctt cttcgtagaa
    17461 acggaggata tcattcatcc agcccatgtt ccatttgtag tcaaatccta gaccaccaat
    17521 ctctttcatt cccgtaatct tggttgcaga cgaactttct tctgcaatca tcatcacatc
    17581 tggatattct aacttaataa cttcattcaa gcgttgaagg aaataataac cttcatagtt
    17641 gagatttccg ccatctttat taggtgtcca tggagcatca tcatagtcca aatagagcat
    17701 gttgctaaca gcatccacac gaataccatc caaatgatag atatcaatcc aatgcttaat
    17761 gcaagaaatt aagaaggact ggacttcatt ttttccaagg tcaaaattaa gggcacccca
    17821 accatggtta tgagccttat tatggtcttg gtattcaaaa gtcggtgtcc catcataata
    17881 ggctaaggca tcatcattga tggtaaagtg acctggtacc cagtccacaa taaccccaat
    17941 attatgggta tgacactcct cgacaaaatc ttgaaactcc tctggtctac cataggcatg
    18001 ctctaaagcg aagtaaccca taagctggta cccccaactc aaacctagtg ggtgggacat
    18061 caagggcata aactcaatat gagtatagtt catctcaact aggtaaggaa tgagttcatc
    18121 tttcaattgg gcaaaactat aaggactgcc atcaggattt ctcttccaag agccagcatg
    18181 aacctcgtaa atattgacag gacgttcagc aaagccccaa cgttttcttc gtgccagcca
    18241 aagtccatcc ctccatttct tatcaggaag ctctgttacg attgcccctg ttcctggacg
    18301 agcctcatac ctgacagcaa aagggtcaat cttcatcagt tgatgaccat tttgacgtgt
    18361 gacatgatat ttgtaaatat gcccttcttg agccatattg gtaaagactt cccagacccc
    18421 aaagtcattt cttaccattg gaatctgatt ttcaatccaa ttggtaaaat caccaaccaa
    18481 gtgaacagcc tgagcattag gtgcccaaac acggaaggta tagccatact ctccatttag
    18541 ttcttcccta tgtgctccta gataatgttg gagataaaaa ttttcacccg tcataaaggt
    18601 ttttaatgct tctctattat ccatatactc cctttctcct gtaagcgttt tctatgtttt
    18661 tattatacta cctttttaga gaagattcaa gtaaattact atacttcttt aattattttg
    18721 aaaatctaca acaagttcac ttactcgttc aattgtaaat caatattttt tcaaaaaatt
    18781 gcgaaaacac ctttcttttt ctactatagt gaaatgaaat aaaacatgcg caaatcgatt
    18841 aaggaattta atctaatttc taacaatgtc ttagaaatca aagtgtact
//
LOCUS       DAVBCV010000042        17718 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBCV010000042 DAVBCV010000000
VERSION     DAVBCV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.042"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(122..1972)
                     /locus_tag="VQ516_001453"
     CDS             complement(122..1972)
                     /locus_tag="VQ516_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2511878.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2040..3179)
                     /locus_tag="VQ516_001454"
     CDS             complement(2040..3179)
                     /locus_tag="VQ516_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2511879.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            3237..4403
                     /locus_tag="VQ516_001455"
     CDS             3237..4403
                     /locus_tag="VQ516_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2511880.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            4542..4868
                     /locus_tag="VQ516_001456"
     CDS             4542..4868
                     /locus_tag="VQ516_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2511881.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            4855..5448
                     /locus_tag="VQ516_001457"
     CDS             4855..5448
                     /locus_tag="VQ516_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2511882.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5441..6352
                     /locus_tag="VQ516_001458"
     CDS             5441..6352
                     /locus_tag="VQ516_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2511883.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6415..7479
                     /locus_tag="VQ516_001459"
     CDS             6415..7479
                     /locus_tag="VQ516_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511884.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7551..8411)
                     /locus_tag="VQ516_001460"
     CDS             complement(7551..8411)
                     /locus_tag="VQ516_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2511885.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHIYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTETDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(8533..9519)
                     /locus_tag="VQ516_001461"
     CDS             complement(8533..9519)
                     /locus_tag="VQ516_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2511886.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9791..11266)
                     /locus_tag="VQ516_001462"
     CDS             complement(9791..11266)
                     /locus_tag="VQ516_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2511887.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11370..12293)
                     /locus_tag="VQ516_001463"
     CDS             complement(11370..12293)
                     /locus_tag="VQ516_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2511888.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12307..13236)
                     /locus_tag="VQ516_001464"
     CDS             complement(12307..13236)
                     /locus_tag="VQ516_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2511889.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13555..13905)
                     /locus_tag="VQ516_001465"
     CDS             complement(13555..13905)
                     /locus_tag="VQ516_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511890.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
     gene            complement(14331..14486)
                     /locus_tag="VQ516_001466"
                     /pseudo
     CDS             complement(14331..14486)
                     /locus_tag="VQ516_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(14672..15283)
                     /gene="rpsD"
                     /locus_tag="VQ516_001467"
     CDS             complement(14672..15283)
                     /gene="rpsD"
                     /locus_tag="VQ516_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2511891.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(15478..16530)
                     /locus_tag="VQ516_001468"
     CDS             complement(15478..16530)
                     /locus_tag="VQ516_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2511892.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(16527..17225)
                     /locus_tag="VQ516_001469"
     CDS             complement(16527..17225)
                     /locus_tag="VQ516_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2511893.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(17392..>17718)
                     /locus_tag="VQ516_001470"
     CDS             complement(17392..>17718)
                     /locus_tag="VQ516_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2511894.1"
                     /translation="VKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSS
                     TNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLK
                     NKKEEN"
BASE COUNT         5256 a         3831 c         3281 g         5350 t
ORIGIN      
        1 aaccatatac ggcaaggcga cgttgacgtg atttgaagag attttcgaag agtataagtc
       61 tctacataat aaaacgcata ttaccaaggt tatttaactt gataatatgc gtttttcttt
      121 tttattcaat gtgggttgtt tgattagcaa aggcgataat agcttgcttc aactcatctc
      181 cactgagagt gcggaactct ccaatctttt gattgatgga ttctaaaggc atgacattaa
      241 ccttaccaac gaaaatctta tccataactt gattatcaac gagttcggtt gataccaaga
      301 gttcttcgta gagtttttca cctgggcgga ttccaacttc aacgattgga atttcacttt
      361 cagtgtggcc acttagaagc accatcttct tggccaagtc ataaatcttg actggtttgc
      421 ccatatcaag gataaagact tccccatctt tggcataagc accagcatgg ataaccagac
      481 ggctagcttc tggaatggtc ataaagtaac gggtcatgcg gaagtctgtc accgttacag
      541 gcccaccttc agcaatctga cgttcaaaga ctggaatgac actaccacgg ctaccaagaa
      601 cattcccaaa acgaactgca cagtaggttg attggctacg ttggttaaag ccagtgacaa
      661 tcaactccgc cacgcgcttg gttgctccca taacatttgg tggattgact gccttatctg
      721 tcgaaatcat aaccatctta ggcaccttgg cttcatcaac ggctctagca acattgtaag
      781 ttccacggat attgtttttg aaggcttctt ttggattgcg ctccatcata ggaacatgct
      841 tgtgggctgc cgcatgataa acaatagcag gtttgtactg ctcaaagact tgcaacaaac
      901 gatcatagtc ttgaatgtcc gcaatcacag gtacataatc aatcccttgg aacttacgaa
      961 tcaattcatg ataaacaagg tagattgagt tttccccatg accgagcaag acaatgcgtt
     1021 caggattgaa gcgactaact tgacgacaga tttcagaacc gattgaacct ccagctcctg
     1081 tgactaagat ggtcttacct gtcagttctg cacccagacg cgattcgtca agacggattt
     1141 cctgacgacc caaaaggtcc gtaatatcaa ttttttggaa gccagtacct gcttggtgaa
     1201 ggccctgaac aacagtttca accttaggca tcttgtaaca tttgacaccc agcttattac
     1261 acatctgcaa gatacgctca tattctgacg gatccagcga cggaatcgca acgatgacac
     1321 gctcgatttg atggcgtttg gctaattcag gcagattgtc ataagagccc aaaacaggaa
     1381 taccaccaag tttttgaccc tttttcttag aatccttatc caaaataccg accagttcta
     1441 attcactggt tggatgttgg taactatcca taaagagagc cccaccatca ccggcaccaa
     1501 tcaagaaggt ccgacggtgt tctccatcac cactaccttt tttgcgtctg gagtagatta
     1561 actgccaagt aatccgtggc aataaaatca agaaggtact caacaagata aagagaatga
     1621 taaaacggat ggagaagagt ggcaagaagg cataacagat actatatgac aagacactgc
     1681 tagcagtcac accaaaaaag attttcatga aatccgtaat cttgctgtaa cgactaatac
     1741 tcgcgctcaa gccccaaaaa tcaaccatca attgatagaa caggaaggcc aaactcgtat
     1801 agataatgta gtcaacaggt gctggattaa tcagcccata aaataaaata taagatacaa
     1861 tgatggaaac caccatactg aaaatatcaa atattcccca gaaaacctgc ttttgttgtt
     1921 tatttaaaat ttccaccaga tcaatcacat aatctgtgag ttttttattc atagaaattt
     1981 actcctcctt aaaatagttc tactatattg ttattatagc acattttaat ttatttggct
     2041 tatttttcca aataaattac tgttcccttg tactccttct tccatttaaa tcctctaatt
     2101 aaacctacaa tcgtcccaag gccataagca aagtgaatgg aaaataaaag aaagggcatc
     2161 acaattagaa atccattttt atgttttaat aaagtcagca aagtgagtaa tgacagtaat
     2221 gataaaagta gaaaataggc acctaatagt aaagttatga atacgaatgt gatcggtaac
     2281 aatgctagac taaacacaag actcaaaaca aataaacaag gaacataatg aaataatgat
     2341 aaacacttaa actgaacatg acttgtcaag ccaatccaca aaccatttga atacttttga
     2401 tgcagcattt tcttgaatgt tggtcgaata tactgataag atagaatact tgggctatag
     2461 cggattttat aaccatgttc tcgaattcta taatgaatat cattatcttc agttcggcca
     2521 agttgctcat ttactaaacc aaccttctgg aaaacctctc gtttatacat cccatgaaaa
     2581 atagaagaaa catatctatc ctcggaacta tttcgataat tggcaatgct actgccaaac
     2641 atattttcct caacaagatg caaggtctct gcccattttc cttttccttt gacaatcgtc
     2701 ggtctaggcc ctccacagac aaattcacct tgttgaataa cagccacatt gttcattaca
     2761 aaactctcag taacttttga atgagcatca atttttaaaa taaggccccc tacagaatgc
     2821 ttaactccca ggttaaaacc actagcttga tttttcttag gattgttata caatctaatt
     2881 gagttaaact ctgcatcttc ttttataaat tgctgaatga tagctgtggt cccatctgtg
     2941 gacatagcat ttataaatag aatttcaata ttctctttag gataggtttg attttttaag
     3001 tcttcaatta gaccaggcag atatttttct tcattgtatg cactgattac aatggatacg
     3061 agaagacttt caacattttg agtcactact actctcctga attttccctt ccaccattct
     3121 atcataattt tcaagacttt tccattttta tcttgaaagc cagaaacctt acttgacatt
     3181 atagtgaaaa agccttaaaa taagctgtta ttaaaatcag ctagaagaga tattatatga
     3241 aaaagcaatc actctttttt gttccaggta ttatcctgat tggtgtttcc ttgcgaactc
     3301 cttttactgt tttacccatt attttgggaa atatttcgca aggtctggag gtagaagtta
     3361 gttcgcttgg tgtcttgacc agcctgcctc tccttatgtt taccctcttt tcaccatttt
     3421 ctacccgact ggctcagaaa atcggcttgg agcatctctt cacctacagc ctcttcttct
     3481 tgaccatcgg ctcacttatt cgactaatca atctgcccct gctctatcta ggaaccttga
     3541 tggttggggc aagtgtcgca gtcatcaatg tcctgcttcc tagtcttatc caagccaatc
     3601 aaccaaagaa aattggtttt ctgaccacct tatatgtaac gtctatgggg attgcaacgg
     3661 ctctggcttc ctatctagct gtgcccatta cacaagccag ttcttggaaa ggacttatcc
     3721 tcctcctcac gttactctgt ctagcaactt ttttggtctg gctcccaaat caccgctata
     3781 atcatagact agctccacaa accaaacaaa aaagtcaaat aaaggtcatg cgtaataaac
     3841 aggtttgggc aattattatc ttttcaggtt ttcaatcctt gatcttttac accgccatga
     3901 cctggttacc taccatgtct atccatgcag gtctatccag tcacgaagct ggcttgctga
     3961 cttctatctt atctctgatt agcattcctt tttcaatgac catcccaagc ctgacaacca
     4021 gtttatctac tcgcaaccgt cagctcatgc tcactctggt ttcactagct ggtgtggtcg
     4081 gcatttccat gctctttttc ccaatcaata atttcattta ctggcttgcc atccatctcc
     4141 tcatcggaac cgcaaccagc gccctcttcc cttatctcat ggtcaacttt tcactcaaga
     4201 ccagcgcccc tgaaaagaca gcccaattgt ccggcctatc tcaaacagga ggctatatcc
     4261 tagcagcctt tgggccaacc ctctttggtt acagttttga cctattccac tcttgggtac
     4321 cagctgtagc tgccctcttg ctcatcgata tcctgatgac tgtggccctc tttacagtgg
     4381 acagagcgga taagatcctt taaacttcta gttttagcta gaagtttttt atatataaaa
     4441 attttacaca tttctcttga cagggctttc ttttagatgt acaatgtatg tgtagaaaaa
     4501 ttatatataa aaatcttaca cattagaaaa ggaggtttcc catgtacttt ccaacatcct
     4561 ctgccttgat tgaatttctc atcttggctg tactggagca gggtgattct tatggttatg
     4621 agattagcca aaccattaag ctgatcgcta atatcaaaga atccacactc tatcccattc
     4681 tcaaaaaatt ggaaggcaat agctttctga caacctattc tagagagttc caaggtcgca
     4741 tgcgcaaata ctactccttg acaaacggtg gtatagagca gctcttgacc ctaaaagatg
     4801 aatggacact ctatacagac accatcaatg gcatcataga agggagtatc cgccatgaca
     4861 agaactgaat acctgactca gctagaactc tatctcaaga aactacctga agctgaccgt
     4921 atcgaagcca tggactattt cagagagctc tttgacgatg ctggagtcga aggagaagaa
     4981 gaactcatcg ctagtttggg aactcccaaa gaagtggccc acgaagttct atccaatctt
     5041 ctcgataaaa aaatcaatga agcacccgct caaaaaaata accgacaaat tttacatatc
     5101 gccttgttag ccctccttgc agcacctatc ggcattcctc tgggaatcgc catcctcgtg
     5161 accctgttcg caatccttgt agccgctttg actgtcattc tggctttctt tgcagtttcc
     5221 atactgggta tcatcggcgg attcctattt ttagttgaaa gtttcactgt cctcgcccaa
     5281 gccaaatcag cctttatctt gatttttggt tctggtttac tggctatcgg tgcttcttcg
     5341 ctagttttac ttggcatttc ctatgtagct cgcttcttcg gtctactcat tgttcgtctg
     5401 gtacaatttg ttcttaaaaa aggaaagaga ggtaatcagc atgcgtaaat ggacaaaagg
     5461 atttctcatc tttggtgtgg tgactaccgt tatcggcttt atcctgcttt ttgtaggtat
     5521 ccaatctgac gggattaaga gcctactttc catgtccaaa gaacctgtct atgatagccg
     5581 tacggaaaag ctaacctttg gcaaggaagt cgaaaaccta gaaattactc tccaccaaca
     5641 cacgctcacc atcacagact ctttcgatga tcaaatccac atttcttacc atccatctct
     5701 ttctgctcac catgatctta tcaccaatca gaacgataga actctgagtc tcactgataa
     5761 gaaactgtct gaaactccgt ttctctcttc tggaattggt gggattcttc atatcgcaag
     5821 tagctactct agtcgttttg aagaagttat tctccgacta ccaaaaggga gaactctaaa
     5881 agggatcaac atctcagcca atcgcggaca aaccaccatc ataaatgcta gccttgaaaa
     5941 tgcgaccctc aatacaaaca gctatatcct ccgaattgaa ggaagtcgta tcaaaaacag
     6001 taaactcaca acgcccaata tcgttaatat ctttgataca gttcttacag atagtcagct
     6061 agagtcaaca gagaatcact tccacgctga aaatatccaa gtccatggta aggttgaact
     6121 gactgccaaa gattatctca gaatcatcct agaccagaaa gaaagccaac gaattaactg
     6181 ggacatctca agcaactatg gttctatctt ccaattcaca agagaaaagc ctgaatcaag
     6241 aggtacggaa ttaagcaacc cttacaaaac tgaaaaaacc gatgtcaagg atcaactcat
     6301 tgcgagatct gatgataata ttgatctaat atccacacca agcagacgtt gacaaaaacg
     6361 cttacatctg ctactatgga agcatattta tctaactaaa aaggaggttc taccatgaaa
     6421 caagaatggt ttgaaagtaa tgattttgta aaaacaacaa gcaagaacaa gcctgaagag
     6481 caagctcaag aggttgcaga caaggctgaa gaaacgatag ccgatctcga tacaccaatt
     6541 ggaaaaaata ctcaagtaga ggaggaagtc tctcaagctg aagtcgaact ggaaagccag
     6601 caagaagaga aaattgaaac tcctgaaggc ggtgaagcga gaacagaaat agaagaaaag
     6661 aaggcatcta attctactga agaagagcca gacctttcta aagaaacaga aaaagtcact
     6721 atagctgaag agagccaaga agctcttcct cagcaaaaag caaccacgaa agagccactt
     6781 cttatcagta aatctttaga aagtccttat atccccgacc aagctccaaa atctagggat
     6841 aaatggaaag agcaagtgct tgatttttgg tcttggctag tggaagcgat caaatctcct
     6901 acaagtaagt tggaaacaag tatcacacac agttacacag cctttctctt gctcattctg
     6961 ttttctgcat cttccttttt ctttagtatc tatcacatca aacatgctta ctatggacat
     7021 atagcaagca ttaacagtcg cttccctgag cagctagctc ctttaactct tttttctatc
     7081 atctctatcc tagtagcgac aacactcttc ttcttttcat tcctcttggg tagtttcgtt
     7141 gtgagacgat ttatccacca ggaaaaggac tggacgctag acaaggttct ccaacaatat
     7201 agtcaactct tggcaattcc aatcttcctc actgctattg ctagtttctt tgctttcttt
     7261 gatagcctac gatttacggc cctcttgtgt gtgattagca ttggaatcat tctgcttgcc
     7321 agtctccata tcattacaag accaagtcaa gcaagtgaaa ccgactcctt ctatcaatta
     7381 ttcttgtctg tccttgtgaa cggagttatt atcctcctct tctttgtagc tgaagtggca
     7441 ctgattggag attatcttcg tatcttggcc tttctttaaa attcaatcct gtcatccttg
     7501 gcaggatttt ttctatgtca aaaaagcaag tcgattgacc tgcttctgct ttacttttct
     7561 tgtgctaatg ttttaaaatc tttgtctaat atgggttgca cttctatttg attggcgtct
     7621 agttggtggt aaaaagatgg gggtaatacc tctaagaatt ttgcatagtc caagcgagca
     7681 atggcttcat agtcttctgg tttagagcca tctcctgtga tttgaaccaa gtcaatctcc
     7741 cactgcactt ccttatccac caattgctca atataggctg caggaacaaa cggttctctc
     7801 ggtaaaactg tcttgactcc ttgagctaga tggtaaacgc catgcacttt tccttctcca
     7861 tcctgataga aggaaactct cgcaaagtag gcatctgtct cttgaaccgc acgcacacgc
     7921 gcttcaaact gagccaaacc atctgcctct aaaaagcttt tcaagttctc acgactaaag
     7981 aaaataggat taaaaattcc ttggcaaatc gtaaaacgag ctacagggtc tgctagatag
     8041 gcttgaatac ttgcttggaa ataagcttca aaatcaaaac catctagccc ttcaatatca
     8101 gtccctgtat aagcaagcaa agcatctaaa aatgacttga taatctgcca gtctgtctct
     8161 gttagtaggt caggaagatc gacacggtaa gccttttgac catcagcata actgacctta
     8221 aaaagaaatt gtgattgccc cactatcgca cactcaatat actcgagacg attaagaggc
     8281 tgacggagat agactacatc atagatatgt gactccaaac cgtctagcaa tgccaagatc
     8341 gacttggcat tcaaaatctc ttgttctcct aaaatgctct gtttatttgg aataaaaaat
     8401 gttttcgcca taactggact cctttgaaat cgcttacata cagtatacct tatttttctg
     8461 aaagatacag aaacaaactt tagatttttg agtacaaagc atagaaaaac agcccctgag
     8521 gactgtttga tcttatacag tagctgcttg atccaagctt tcaccgatag cggctaggcg
     8581 ctcgatcact tcagcttgtg tcaattcatt ttttgaaaca tagcggttac gtgggtgaac
     8641 acggcactcg tgtgagcatc cacgaaggta cttgtcttca ttttcttctg atgtcaagat
     8701 acgacggtta cagaagggat ttccacagtt gacataacgt tcacatggtg ttccatcaaa
     8761 ccagtctttc cctacgatgg ttgggttgac atggttgaca tcaacggcga tacgctcgtc
     8821 aaatacgtac atttttccat cccaaagctc accttgaact tctgggtctt taccgtaagt
     8881 tgcgattcct ccgtgcaatt ggccgacatc tttgtagcct tcacggacca tccagcctga
     8941 gaatttctca cagcgaacgc cacctgtaca gtaaaccacg acacgcttgt ccatgaattt
     9001 ttccttgtta tcacggaccc attgtggtaa ctcacggaag ttgcgaatat ctgggcgaat
     9061 agctccacgg aaatgtccta ggtcgtactc ataatcgtta cgtgtgtcaa ggacaacggt
     9121 atctttatca agaagcgctt ctttgaactc ttttggagac aagtaagcac ctgttgtttc
     9181 aagtgggttg atgtcattgt caaagtcgtt gtcttccaaa ccaaggtgga caatttcttt
     9241 cttgtagcga acaaacatct tcttgaaggc ttgttcattt tcttcgtcaa tcttgaacca
     9301 gagttcttcc attcctggaa ggctgtgaac gtagtccatg tatttttgag ttgtttcata
     9361 gtcacctgaa actgttccgt taattccctc gtcagcgact aggatacggc ctttaagacc
     9421 gattgattta cagaaagcca agtggtctgc agcaaattgc tctgcatttt caattggagt
     9481 ataaaggtag taaagtaaga cacgaatatc ttttgccata agatttgttc tcttttctat
     9541 tcttaaattt tctaaatttt ttatttatcc atactagtat acctgcttga gaaaacgaat
     9601 gcaatttatt tgactggaaa ttgtagagga ttctcgaaat ttcctgaatg gttaaaataa
     9661 ggacaagaga aaacatggat atctatatcc ttgtgccaaa aaaaccactg ccctccccag
     9721 accaacctga ggaaagcagt gattcttatt ttaggagtta ggaatgaata cacgaaatca
     9781 atttagctga ttattttttg tttttcaaga attcatcgta ttgtttttgc atttcgttca
     9841 atactttttc gtaggcacct tcagatttca atttttccat caattctgga atcgctttat
     9901 ctgggtctac agtaccagtg ttgatagctg tatcaaattg ttgcattgtg ttagcaatag
     9961 ctgagatttc agatttcaca ttgtcagtat tgaagataaa tccaagcgct ggagattctt
    10021 tagcttctgc caattctttc ttagaatttt cgatttgttg gtctgtaacg ttttcgttga
    10081 tgtaaaggat ccagttgtta ccagtgttcc atccacccat gtgagtgttt cctttgtagc
    10141 catcaagaac gcgaacacgg ttttctttac cttcaatttt ttcccagttc ttgccttctg
    10201 gaccgtaaac aagaccgttc aaaagttctg ggttcgtatt caagaggttc aagatttcca
    10261 ttgatttttc tttgttctta gagttgtttg agatgacaaa gttagcaact tgtgttgttt
    10321 ggtttttctt gatgaagtta gtgattggtt tgatttggat atctttgttg gcaacacgtg
    10381 aaagcaagct gttaccgtag tcagctggtc ctactgtttc ttcacgaacg aaccaagtat
    10441 cttgttgaag gtcaaatgaa gtatcgcttg ttgcgacgtc ttttggaatg tagccagctt
    10501 catagaattt gtgaagagtc ttcaagtgtt ctttgaaacg aggcacttcg taacggttta
    10561 caactttagt agtatcgcct tcaaggtcaa taacgaatgg aagcccgttt gctactgggt
    10621 agtcaaaatt atcagatggg atgaaaactt taccaatagc aaatggtact acgtctggag
    10681 ctttttcttt gatttgtttc aagactggct caagagtttc gtaagaagta acacctgaaa
    10741 tatcgatacc atatttggca aggagagttc cgttgaaggc aaagttttga gatgatgcta
    10801 cgttggctgc aactggtaca gcataaatct taccatttac agtattaccc ttgatataag
    10861 ctggatcaag tgccttgtaa aggtctttac cttctttttt gtacaattct gtcaagtcag
    10921 cataagcacc tttttgagca tttacaatat agttatctgc aaaggcgata tcatagtttt
    10981 caccagatga tgtgataact gacattttct taccatagtc accccagcca aggtattgga
    11041 tatccaattt ggcaccaact ttttcttcga tgattttgtt ggcatttgct aacaattcat
    11101 ccaagttatc tggtttgtca ccgatttggt acattttgat aacaggtttg tcacctgaat
    11161 cagcagcttt tttgctgtta cctgtcaaat ttccacaagc agcaagacct gcagccagag
    11221 cgactacact agcagatgca aaagcatatt ttttccagtt tttcatgata aaaactcctt
    11281 tttttatttt taaacttata aacaatgtaa tgatcttaac ttcacacaag ggaagttggg
    11341 aactgagaaa tgttcttttt caataagcac tattctttca caccaccgat agtcaaacct
    11401 tttacaaagt agcgttggaa gaatggatac aaaatcgcga ttggaagggt tgctaccaca
    11461 accatggcca tacgacctgt ttctttcggt agagcaactc ccagttgacc agtcaagccg
    11521 accgctttgg caatgtagtc catattttgt tggatttgca tgagcaaata ttgcaatgga
    11581 tacaagttat cactcttgat gtaaagaagg gcgttgaacc agtcattcca gaaaccaaga
    11641 gctgtcaaaa gcgtgatggt tgcgatacct ggtagtgaca atggcaaaca gatttggaag
    11701 aaaatccgag cctcactggc accatcgata cgagccgatt cgagaatggc ttctggaatg
    11761 gtcttcttga aaaaagaacg catcaagatg atattgaatg gtgagagaag cattggaaca
    11821 atcaaggccc aaacagtgtc accaagctga agtacacggg tcaccatgat ataacctggt
    11881 accaaaccag cgttgaacaa catactgaga aggacgaaga tggtaaaaaa tctgcgatac
    11941 ttaaaggttg tccgtgaaat agcgtaggca taggttgttg tgataaagac atttgtcaat
    12001 gtcccaacta cggttacaaa gacagagatg aagagggctt gtaggatttt atccttaaac
    12061 tgtgccaaaa actcaaaacc gtctaagcca aattgggatg ggaagaagct atagccgtat
    12121 tggaggaggc ttttctcgtc tgtcactgaa ataatgataa cgaatacaaa aggtaggata
    12181 caagagaggg caatcaaacc cgaaatgata ctgaagaaga tatctgcttt cttactgaag
    12241 gagtgaatgc cgacattatc aattttttct tttttaattt tcttttttgc catattctcc
    12301 tcctttctag aacaaagctg agtttggatc gactcgtctt gcaagcaagt ttgataggat
    12361 aaccagaatc aaaccaacaa cggattggta aagaccggct gctgcagcca taccgatatc
    12421 tgctgtctga gtcaaaccat taaagacata tacgtccaaa acgttggtta cattgtaaag
    12481 ctgaccagca ttgtgtggga tttgatagaa gagaccgaag tctgcgcgga agatatttcc
    12541 gactgcaagg atggtcaata cagttacaag cggagtcaac tgaggaatgg ttacgttgcg
    12601 aatacgttgc cacttgctag ctccgtccac tgtcgctgct tcgtagtagg ttggatcaat
    12661 tcccatgatc gtcgcatagt acatgacact gctatatcca aagcctttcc aaatacctag
    12721 gaaaagtagg agatagggcc agatgcccag gtcagcgtag aaattgactt ctttgagacc
    12781 aagactttcc aatagatgat tgaacacccc tttatcaata tttaggaagg catctgtaaa
    12841 gaaactgatg ataacccaag acaagaagta agggaacaac atagaagttt gaaaaatctt
    12901 caccattctc ttagaacgga gctcgctgag gataatggca atccctacag atacaactaa
    12961 acctagaaag ataaagccaa gattgtagag gacagtattt cgtgtgataa taaaggcgtc
    13021 tcttgaacta aataagaatc tgaaattatc gagtccgacc catttactat ttatgatact
    13081 atctatgaaa ccattactgg tcatgtggta gtctttgaag gcaaccacgt tcccaaatac
    13141 tggaatgtaa aagaatagaa tcaaccagag tgcccctggc aaaaccatca agagaaagat
    13201 ccagttgtct ctcaatgttt ttgaaaactt tttcataatt tcctcccttt ttattttgat
    13261 atccatctaa aaattctttt ttagactttt gataacgatt acattattag tatactccta
    13321 tttgcaggtt aggttaaact cctaattata gaaaaaactc cacaaattat gtagcagatt
    13381 taaaacttta tcaccactat caaacaaatg tcctaaatca attgtttatt ttatctctat
    13441 tagcccagtg atggcgtcac tctgttataa gcatccaaca acggggtata ctgaaaaatc
    13501 tccagactag ggaactcagc gatagttcct aatctggaga tttttaatat gttattaggc
    13561 gtttgctttc aacttagcaa taacctcttt aagattatca atcaactctg cttcagtatg
    13621 ctcagagcct ttttcatctg ccaagaacaa aactgctttt tgaagttctt cttgagagtt
    13681 ttcaaggaca tccttatctg ctgtttcttt gcccgctgtt tgtcctccat ccttagttgc
    13741 tgactggctt tccttaatgg actctaggga agcaatggca tctttgactg tttgcaagat
    13801 atcacgtaaa ccttgctctg tcaaactatc atctgcaaaa gctttcttag cctctgccaa
    13861 aaccagacgt gctgaatctg tggtaggatt cgatacacct gtcaatgatc tcaaaagatt
    13921 ttctaaagtt tgagtctgct tactaatact agactaaaat caaaaagcat tatataacag
    13981 tgatatgaaa tcaactaaag aagaaatcca aactatcaaa acacttttaa aagactctcg
    14041 tacagctaaa tatcataaac gccttcaaat cgttctatag taaaatgaaa caagaacagg
    14101 acaaatcgat caggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact
    14161 attctagttt caatccatta tatttcgtct gatgggcaaa tcttataaag agattataga
    14221 acttttatag tagtttgaaa taagatgtga acaactctat caggaaagtc aaattaattt
    14281 atagaaatat tttagcagct aaggtgtact gttatagatt caatacacta tacttggtgg
    14341 tttagctctc ctgttttctc ttttagcttt aaccagccat aaatggtatt acgtgagatt
    14401 tggaaaacgt gtgatgcttc tgttatacta cctgttcgct cacaataaga gagaattttt
    14461 ttacgaaaat ctattgaata tgccataaaa ggattatacc acattgtgta ctatttttgg
    14521 ttcattttac tatatcactt tggactatat cggtctagtt atgtctaaac gattatagct
    14581 ggatactaag agaaatcaaa aaagcactaa gggagaacgt cccaaagtgc ttaatatcaa
    14641 ggcctcaaag cctgtcttat tcaataaaat attacaacat cttgttgtag aattcaacga
    14701 caagtgcttc gttgatttct gggttgattt cgtcgcgttc tggcaagcga gtcaatgaac
    14761 cttccaattt ttcagcgtcg aatgatacga atgctggacg tccaagagta gcttctactg
    14821 cttcaaggat tgctggaact ttcaatgatt tttcacgaac tgagatcact tgacctggag
    14881 ttacgcggta tgatgggata tcaacgcgtt tcccgtcaac aaggatgtga ccgtggttta
    14941 cgaattgacg agcttgacga cgagtagtcg cgagaccaag acggtaaaca acgttatcca
    15001 aacgacgttc caaaagaagc ataaagttga aacctaggat tccgcctttg atttttgtag
    15061 cttgtacgaa caagttacgg aattgttttt cacctacacc gtaagtgaaa cgaagttttt
    15121 gtttttcagc caattgcaaa ccgtattctg acaatttaga acggttgttt ggtccgtgtt
    15181 gtcctggtac gtagttacga cgtgccaatt ctttacctgt acctgtaagt gaaaggccaa
    15241 ggcgacgagc ttgtttccaa gatggtcctg tataacgtga catatgaatg tcctcctgat
    15301 ataaataata tttcggtgga aatagtcact tagaaagccc tgattcgtgc agatgccctt
    15361 cgcctaaaca gccaaggtta cttgtcataa gacacctgtt gacgagcttc atgctttcct
    15421 gctgatattt cacacaaaat ccattttacc atgaatagct ggatttgtaa aggggtttta
    15481 ggctttattt tcactaccag agagattgag aacgagggta aaggtacttc ctagaccgta
    15541 ctggctgctg actgtgattt ccccacccaa ttgatgggcc aattcacgcg caatcgcaag
    15601 tcctaatcca tgaccacctg tcttcatgtt acgcgaagtt tcgacacgat aaaggcgttt
    15661 gaaaatattt tccaaatcct ctggggcaat gccctgccct tcatcggtca cactgattga
    15721 aagctggccc ttctccagct tagtcaccac ttccagcttg gttcctggag cagaatattt
    15781 aaaagcgtta tcgaccagat tcaccaagat acgagaaagc ttagcataat ctccctcaat
    15841 ccgggcagac tctgggatta cctgcaagtg gacatctctt ctctcctgct caatcaaaaa
    15901 ctgaaattca ctcatgcact caattaagag cttgtccaga aaaatactgt ctttactggt
    15961 agtttccacc tgatttctag ctgtgtttag ggtcaaaaaa ttcaactcct caaccagttt
    16021 attgagcctc tccgtctggc gtccaatggt tgctagataa tgagcttgct ccgactcctt
    16081 gataatccca tccaaaatcc cttctaccgt cgcttggatc gaagtgatag gggtcttaat
    16141 atcatgcgac aactgggcaa tcatcaagcc cttttctcgt tcgctttctt ccaaggaatc
    16201 aaagcttacc tgcaaatcat gggacatctc attaaaagtt tgccctaatt gctgaaattc
    16261 tacaggacct tgaacctcca aatttgaagg aaaatccttg gccgctaccc gcttggcatg
    16321 ctccttgagt ttgcccaacg acgtaaagac tggcaatagg agaaagagac taatcccagc
    16381 accgacaagg ctggcaacga tggtcatccc aagcaaaaag taaatctcgc ctttcgcaat
    16441 cagcattttt tgaacagccc aaaaaacaac caaaatggtt aagagggttg aaataatata
    16501 tccaaccaaa atataacttt ttagtttcat gtttgtcctc tcggtttctc tatcttatat
    16561 cccaaccccc aaactgtctt aatagtgggc gtttggtcac tactatattt tgccagctcc
    16621 tgtcgaagag catggatatg cacattcaag gtattggtgt catccacgta gtcttctttc
    16681 cagatctttt catagaggtc tgtcttggag aaaactcgct ctggattgct agctaaaatc
    16741 cacagcaatt caaatgattt aacagttaaa tccagcattt cttctcctat ttgaacttca
    16801 tgactactat gattcatttt tagattgcca agggaaatca gctctgtttc gcccccacga
    16861 tgaaggcggc gcaaaatatt gtggacacgc aaaaccagct cacgtgggct aaaaggctta
    16921 gcaataaaat catctgctcc caagctcagg ccgtaaatct tgtcctgttc actggtctta
    16981 gcagtaataa ataggaaagg ctgctctggt gataagtatt gaacctcact gattaaatca
    17041 taaccatcca tccgaggcat cataacatct gtgataatca aatcaatcgg ttttttcttg
    17101 aacagctcta gcgcttccag tccatcatgg gctaccaaga cctgataacc tgcctgaatt
    17161 aagtatctct gatgaatatc tgtgatttct acctcgtcgt caacgagtaa aattgtcttt
    17221 cccatctgtc tctcctttga taaaaacagt gctatactgc tttaagtata acactatttt
    17281 tattgtttta cgatgatttt aatctgctat tgaaagaaag tcctgattgc tgattgagtt
    17341 gttaaactaa tcttagtcag cccactatcc atcatttatt tccttagctg attagttttc
    17401 ttcttttttg tttttcaaac ctagaccacc gagtaaacct gcaagcgcaa gcccaaggaa
    17461 accaatactt gccattgatg tttgagtctc accagtattt ggtagcatag ctttatcctc
    17521 tgacatcatc gtatcagaca tcttgttagc agaagcagcc atgttttcac ctgccatcgt
    17581 gttggtagaa cttgtcatgg tgtcagcagg catgctatct gtagtacctg tagcatgatt
    17641 gtgattcatc ggagtcacgc cagaaccaga gttagaaggt gataatgaac catttgctgt
    17701 gtctgatgtt tctttaac
//
LOCUS       DAVBCV010000043        17572 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBCV010000043 DAVBCV010000000
VERSION     DAVBCV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.043"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(179..1114)
                     /locus_tag="VQ516_001471"
     CDS             complement(179..1114)
                     /locus_tag="VQ516_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2511895.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1111..1971)
                     /locus_tag="VQ516_001472"
     CDS             complement(1111..1971)
                     /locus_tag="VQ516_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2511896.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2079..4241
                     /locus_tag="VQ516_001473"
     CDS             2079..4241
                     /locus_tag="VQ516_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2511897.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4333..5592
                     /locus_tag="VQ516_001474"
     CDS             4333..5592
                     /locus_tag="VQ516_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2511898.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRSE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5606..6472
                     /locus_tag="VQ516_001475"
     CDS             5606..6472
                     /locus_tag="VQ516_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2511899.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6486..7319
                     /locus_tag="VQ516_001476"
     CDS             6486..7319
                     /locus_tag="VQ516_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2511900.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKSTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            7320..7400
                     /locus_tag="VQ516_001477"
                     /pseudo
     CDS             7320..7400
                     /locus_tag="VQ516_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7538..8980
                     /gene="gtfA"
                     /locus_tag="VQ516_001478"
     CDS             7538..8980
                     /gene="gtfA"
                     /locus_tag="VQ516_001478"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2511901.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTEILPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAREVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVSF"
     gene            9118..10311
                     /locus_tag="VQ516_001479"
     CDS             9118..10311
                     /locus_tag="VQ516_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2511902.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10998..12977
                     /locus_tag="VQ516_001480"
     CDS             10998..12977
                     /locus_tag="VQ516_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2511903.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13044..14540
                     /locus_tag="VQ516_001481"
     CDS             13044..14540
                     /locus_tag="VQ516_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2511904.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14540..15466
                     /locus_tag="VQ516_001482"
     CDS             14540..15466
                     /locus_tag="VQ516_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2511905.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15475..16542
                     /locus_tag="VQ516_001483"
     CDS             15475..16542
                     /locus_tag="VQ516_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511906.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16553..17479
                     /locus_tag="VQ516_001484"
     CDS             16553..17479
                     /locus_tag="VQ516_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511907.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         5175 a         3141 c         3674 g         5582 t
ORIGIN      
        1 aggctccata atatccatag ggaatttacc cactacaaat attatagagc ccgttttaat
       61 gagagtttgt aactctcttt tttgttttta gagacaataa aacttcagat ccgtaaaact
      121 taattgaatt actaatctga agttattttt atatcgttta tattataatt gtgttatttt
      181 acttccaact attgagagaa atttcgccac tatttagcca gatttctttt ccgttatcac
      241 attgaactaa aagttttcca ttttctgaga tgtctttagc aagtcccttg tagtcttttt
      301 gctctagtgt gaaagtgact tcttttccta gaatgaatga ctgttttttg tataggtata
      361 atagctcttc tgctggtgtt tcgaagaaag cacgccagat ttctatgatc aattcattcc
      421 ttgttatagg agctgtagct ttaaataagc tggcagcttt ttcttttaat tcctgaggga
      481 agtctttaat agtgaaattg atacctactc caataatgat atctgtgact aagccagttt
      541 ctacagaggt cattgcttca gtaaggattc ctccaatttt atgattgttt agatagatat
      601 cattgaccca ttttatgtcg acatctatta aagttaggtt cttaatggct ttgtagacag
      661 ctccagctac aagtagtgtg taggatggta atttgtcata ggggagattt ggtttaagat
      721 ggagtgtcat ataaatacca ccttgtggtg agtagaagga acgttgaaaa cggcctcggc
      781 ctgctgtttg ataggaagct agatagaggg tatttgcttc atggcctaaa tcaattgctt
      841 cttttgcatc tagttgtgtt gattttgttt cgggtttaaa gctgacttta attggaagat
      901 tttcttctag aatctctgga agaataaggt caccattcat cagtttatat cctctatttt
      961 tgatactatc aatttcaatg ccttcttgtt ctagtcgctt gatggctttc caaattgctg
     1021 ttcggcttag ggatagtttt tctgcgattt tttctccgct gatatagtcg gtttctttag
     1081 ataggatttg gtagacagct tggtaggatt tcataatgtt gcctttctca ctagttggta
     1141 ttgagagtat tcttttcttg tatgacttgg agtctgatta aagtattgtt tataagcttt
     1201 cgaaaaatgg agtggatctg aaaaacctac cgagtatgca attaccttga tggactcttg
     1261 ggtattttcg agaagttgtc tagctcggtg cattcgaacg tagagtaggt attctttggg
     1321 tgataaggta ttaaattctt tgaatacgct tgataagtag cttctgtgaa cggatagttc
     1381 ttttgctaaa tcttgaattg taagtgattg aggatagtgg ctatcaatta atcgtttgca
     1441 ttcaagatag agttggtggg ttgatgaaat attctttttt ttctgattgg gagcaatagt
     1501 tcccagatga aacatcagtt catgaagttg tcccatgata tggagttgag ctaattcact
     1561 tgattttgta atctgagcga agcggacaat gtctgagatg agttttgcag tagtctgggt
     1621 atgacaagtt tcagattgga tgagatagga ttgatcagaa atttgagaaa gagcaaaata
     1681 atcaggggat ttccctccag tgattcctaa ccagtagtag gcccaaggtt ctttactatc
     1741 tgcttgataa aaggttagtt cctctggttt taatagaaag aaatctcctt cttttaaatc
     1801 aacaatttta cccttgtaat aaaattttcc ttgtccttta gtaatgtaat gtagaacgta
     1861 tgtatcacga atggctggac caaaagagta attaggtgtg cattcctcat atccataaaa
     1921 gcttagggca aggtcgattg ttccagtctg gtattctgaa aaaactaaca tgtgctacct
     1981 cctacctaac attttaccat attcttgaga catttttcta ttttggaaag cgatttcagg
     2041 tgataaaata taatcaataa agtgatgagg tgaagtaaat gggagttagg atagagaata
     2101 atctatttta tgttgagagt aaaaatctaa gtttgattat tgaaaatcga aatggctact
     2161 tacttttgaa acatttagga aagactatta agaactataa aggttccaat agtgtttatg
     2221 aacgagacca tgccttttca ggaaatccaa cggctactaa tcgaaccttt agtttagata
     2281 ctcagcgaca gatttttgga caacatggct taggagattt taggaaacca actatacagg
     2341 ttcagcatag tgtaactgaa gtaacagact ttcgatttgt agaagcaaag attttaaaag
     2401 gtcagaatgg tccacagggc ttaccttctc cacatagcat ggacgataca gagactcttg
     2461 tcttaatgtt agaagattct aaggctcaac ttagtctgac tttgtattat actactttta
     2521 ataatgatgc gactattact agctacagta aattagataa taatagtaat caggaagttg
     2581 tcatccataa agatttttct tttatggctg attttccagc tgcagattac gaaatagcaa
     2641 ctctgcaggg tgcttatgct cgtgaaaaga ctgttagacg tcaacaggta gaacaaggaa
     2701 tcttttcgat tagttcaaac cgaggtgctt ctggtcatgc tcaaacacca gctcttctac
     2761 tatgcgaaca aggagtcaca gaggatgctg ggaatgtgtt tgctattcaa ctaatgtata
     2821 gtggcaactt tgaagctttt gttcaaaaaa atcaattgaa tgaagttcgg gtggctattg
     2881 gcattaatcc agaaaacttt tcttggaagt tagcccctga ggaatacttt gaaacaccgg
     2941 tagctttagt gactcattca gatcagggat taactggtat tagtcatgaa agtcagaatt
     3001 ttgtactgaa gcacattatt ctaagtgaat tttctaaaaa agaacgtcca attctaatca
     3061 ataactggga agctacttac tttgactttc agagagaaaa actgttagag ttagcagatg
     3121 aagctaagaa agttggcatt gaactttttg tattagatga tggttggttt ggcaatcgtt
     3181 ttgatgataa tcgtgcttta ggtgattggg ttgttaatga ggaaaaactg ggtggaagtc
     3241 tagaaagtct gatttcagct atccatgaaa gaggtttgca gtttggactt tggttagaac
     3301 ccgaaatgat ttctgtagat agtgatttgt atcgtcaaca ccctgactgg gctattcagg
     3361 ttcctgacta tgagcatact tattctcgga atcaattagt acttaatctt gccaatcctc
     3421 aggtagtaga atacttgaaa agtgtcttag atcaactcct atcttatcat gagattgatt
     3481 acattaaatg ggatatgaac cgcaatatca ctaagctagg gaatggatta acttatctag
     3541 agacacagat gcaatctcat cagtacatgc tggggcttta tgaactcgtt tcttatctga
     3601 cagagaagca cagccatatt ctctttgagt cctgctctgg tggtggtgga cgaaatgatc
     3661 ttggtatgat acgctatttc ccacaagtct gggctagtga taatactgat gccattgcac
     3721 gtttaccaat tcaatacggt tcatcctatt tctatccaac catttctatg ggggctcatg
     3781 tgtcagcagt accgaatcat cagatgggac gaatgacacc attagaaaca cgtggccatg
     3841 tagcaatgat gggaaatttg ggctatgagc ttgatttgac aaatttatca gatgaagaga
     3901 aagctacgat tgctaatcag gtgaacttgt ataaagaatt acgaccagta gttcagttag
     3961 gacagcagta tagactaatt aatcctgata ctgcatccaa tgaagcagct gtacaattta
     4021 attacggaaa tcaaacgatt gtaacctacg ttcgcgtttt atctgttgta gagaccatgg
     4081 aaacaacttt aaaattaaaa gatttggatg aagagggact atataaatta caggaaaatg
     4141 gcgaagttta ctcaggtgca gaactaatgt atgcgggctt aactgttatc ttatcccaag
     4201 gagatttttt gagtagacag tatattttta gaaaactatg atatgtactg accccaaaag
     4261 ttagatttat tctatctaac ttttgaagtg cagtttgata agggtgtata aatttataaa
     4321 ggaggctttc taatggaatg gtataaaaaa atcggacttc ttgcaactac aggtttagct
     4381 ttgtttgggc tcggtgcttg ctccaactat ggtaaatctg cggatgacac agtgaccatc
     4441 gagtatttca accagaaaaa agaaatgacc aaaaccttgg aagaaatcac tcgtgatttt
     4501 gagaaggaaa accctaagat aaaggtcaaa gtcgtcaatg taccaaatgc tggtgaagta
     4561 ttgaagacac gcgttctcgc aggagatgtg cctgatgtgg tcaatattta cccacagtcc
     4621 atcgaactgc aagaatgggc aaaagcaggt gtctttgaag atttgagtaa caaagactac
     4681 ctgaaacgcg tgaaaaatgg ctacgctgaa aaatatgctg taaacgaaaa agtttacaac
     4741 gttcctttta cagctaatgc ttatggaatt tactacaaca aagataaatt cgaagaactg
     4801 ggcttgaagg ttcctgaaac ctgggatgaa tttgaacagt tagtcaaaga tatcgttgct
     4861 aaaggacaaa caccatttgg aattgcaggt gcagatgctt ggacactcaa tggttacaat
     4921 caattagcct ttgcgacagc aacaggtgga ggaaaagaag caaatcaata ccttcgttat
     4981 tctcaaccaa atgctattaa attgtcggat ccgattatga aagatgatat caaggtcatg
     5041 gacatccttc gcattaatgg ctctaagcaa aagaactggg aaggtgctgg ctataccgat
     5101 gttatcggag ccttcgcacg tggggatgtc ctcatgacac caaatgggtc ttgggcgatc
     5161 acagcgatta atgaacaaaa accgaacttt aagattggaa ccttcatgat tccaggaaaa
     5221 gaaaaaggac aaagcttaac cgttggtgcg ggagacttgg catggtctat ctcagccacc
     5281 accaaacatc caaaagaagc caatgccttt gtggaatata tgacccgttc agaagtcatg
     5341 caaaaatact acgatgtgga cggatctcca acagcgatcg aaggggtcaa acaagcagga
     5401 gaagattcac cgcttgctgg tatgaccgaa tatgccttta cggatcgtca cttggtctgg
     5461 ttgcaacaat actggaccag tgaagcagac ttccatacct tgaccatgaa ctatgtcttg
     5521 accggtgata aacaaggcat ggtcaatgat ttgaatgcct tctttaaccc gatgaaagcg
     5581 gatgtggatt aggagtagag agactatgaa aaaagtatta caaaaatatt gggcatgggc
     5641 ttttgtggtc atccccctct tgttacaagc aattttcttc tatgtgccga tgtttcaagg
     5701 agccttttac agttttacca actggacagg attgacttac aactacaaat ttgtcggctt
     5761 aaacaacttt aagctcctct tcatggatcc aaaattcatg aatgcgattg gctttaccgc
     5821 aatcattacg attgccatgg tggttggtga gattgcactc gggatcttca ttgcgcgtgt
     5881 cttgaactct aaaatcaaag gccaaacctt cttccgtgct tggttcttct tcccagctgt
     5941 tttatctggt ttgacagtgg ctttgatctt caagcaagtc ttcaactacg gtcttccagc
     6001 gattgggaat gcccttcata ttgaatttct ccaaaccagt cttttaggga ctaagtgggg
     6061 agcaatcttt gcggctgtct ttgtccttct ttggcaaggg gtggctatgc ccatcatcat
     6121 cttcctagct ggtttgcaat ctattccaac tgagattaca gaggcagcaa ggattgatgg
     6181 tgcgactagc aagcaagttt tctggaacat tgaattgcct tacttgctac caagtgtctc
     6241 tatagtcttt atcctagccc taaaaggtgg gctgactgcc tttgaccaag tctttgccat
     6301 gaccggtggt ggtccaaaca atgccacaac ctcacttggg ctcttggttt ataactatgc
     6361 ctttaaaaac aaccaattcg gttatgccaa tgccattgcc gtaatcttgt tcttcttaat
     6421 tgtagtgatt tcgatcatcc aattgagagt atctaagaaa tttgaaattt aagaggagaa
     6481 gcatgatgaa acaagatgaa agaaaagccc tgattggcaa atacattcta ttgattctag
     6541 gatcggttct gattttagtg ccgctccttg ctaccctctt tagttccttc aaatccacta
     6601 aggatattgt agataatttc tttggctttc caaccaactt cacatgggac aactttagcc
     6661 gtctcttagc tgatgggatt ggaggctatt attggaactc tgtcgtcatc actgtcttgt
     6721 ctttacttgc agtaatgatc tttatcccta tggcagccta ctccatcgct cgcaatatga
     6781 gtaaaagaaa agcctttacc atcatgtata ccctcttaat cctcggaatc ttcgtacctt
     6841 tccaagtcat catgattccg attacggtta tgatgagtaa actcggtttg gctaatacct
     6901 ttggtttgat cttgctctac ttgacctatg cgattccaca gaccctcttt ctctatgttg
     6961 gctatatcaa aatctcgatt ccagaaagtc tggatgaagc agcagagatc gatggggcta
     7021 atcaatttac aacctatttc cgcatcatct tcccaatgat gaaaccgatg catgcgacaa
     7081 ccatgatcat caatgccctt tggttctgga atgacttcat gttgccactc cttgtcttga
     7141 accgggattc caaaatgtgg actctgcctt tgttccaata caactacgca ggccaatatt
     7201 tcaacgacta cggaccaagc tttgcctctt acgtggtcgg cattatcagt atcaccattg
     7261 tctatctctt cttccaacgc catatcatcg caggaataag caacggggca gtgaagtaat
     7321 actcaatgaa aatcaaagag caaactagga agctagccgc aggttgctca aaacactgtt
     7381 ttgaggttgt ggatgaaact gacgaagtca gtaaccatat ctacggtaag gcgacactga
     7441 cgtggtttga agagattttc gaagagtata acatgaattt gatttacgaa gccagatcat
     7501 atgagacttg gcttcaatta gaaaaaggag agtaatgatg ccaattcaaa ataaaaccat
     7561 gttgattacc tattctgata gccttggaaa taatcttaaa gacttatatg ataatttgga
     7621 agagcatttt ggagatgcta ttggaggagt tcacctttta ccatttttcc catcaacagg
     7681 tgatcgtgga tttgcgccag ttgactacga cgaagtggac tcagcttttg gtgattggga
     7741 ggatgttaag cgtttaggtg agaaatatta tcttatgttt gattttatga ttaatcatat
     7801 ttctcgtcaa tccaagtatt ataaggacta tcaagaaaaa catgaagcca gtgaatttaa
     7861 agatctcttt ttaaactggg ataagttttg gccagaaaac cgtccgacac agtctgatgt
     7921 agatttaatt tacaagcgta aggatcgtgc accaaagcaa gagattgtgt ttgaagatgg
     7981 ttcagtggaa catttgtgga atacctttgg tgaggagcag attgatcttg atgtgaccaa
     8041 agaagtaact atggaattta tccgtaagac cattcagcac ttggcaagta atgggtgtga
     8101 tttgattcgt ctagatgcct ttgcttatgc agtgaagaaa ttggatacta atgatttctt
     8161 tgtggaacca gatatttggg atttattgga caaagttcga gatatcgctg ctgagtatgg
     8221 gacagagatt ttacctgaga ttcatgaaca ctattcgatt cagtttaaaa tagcagacca
     8281 tgattactat gtttatgatt ttactcttcc aatggtgaca ctttatactc tttacagttc
     8341 cagaacagag cgtttggcta agtggttgaa gatgagcccg atgaagcaat ttacgacgct
     8401 agatacccat gatgggattg gagtagtaga tgtcaaggat atcctgaccg atgaggagat
     8461 tgactatgct tcaaatgaac tctataaggt tggagccaat gtcaaacgta agtactctag
     8521 tgccgagtat aacaacttag atatctacca aatcaattca acctactatt cagcgcttgg
     8581 agatgatgat gtcaagtatt ttctcgctcg tctaattcaa gcttttgccc caggtattcc
     8641 tcaggtttac tatgtgggtc tattagcagg caagaatgac ttgaaattat tagaagaaac
     8701 taaagaaggt cgaaatatta atcgtcatta ctatagcaac gaggaaatag caagagaagt
     8761 gcaacgacct gttgtgaagg cccttctcaa tctattttct ttccgtaacc gttcagaagc
     8821 ctttgatcta gaagggacta ctgagataga gacaccaaca gctcacagca ttgtaatcaa
     8881 acgtcaaaat aaagataagt ccgtaacagc agtagcagaa attgatttgc aaaatcagac
     8941 ttatcgggta attgagaatg gagttgaagt atcattttga agccttgata tggtggataa
     9001 aatagggttt ttattttgga aaacgtttcc tttgttttca aattgctaaa aaagtggtac
     9061 aatataggat agcttactat tatctgaatc agctgatttg gagagaaagg attcattttg
     9121 aaatcaatag gctttattga aaagctgaag gggttgtcta gtaaagagct gattttattg
     9181 ggaattatcc taagtatctt tttacccttt tatctttttg tagttgtact ctgtttatat
     9241 attatcagtt tgatttttac aggagacatg aaaagtattc ttcagaaaat gggggagcat
     9301 ccgatgctgc ttctttttct tagctatagt actgttatat ccattcttgc acaaaattgg
     9361 atgggtcttg tggcttcagt aggaatgttt ctatttacta ttttcttttt gcactatcag
     9421 tcgattttat cccataaatt ctttcgattg attttgcagt tcgtcttgtt tggtagtgtc
     9481 ttgtcagctg cttttgccag tttagaacat ttccaaattg tgaagaaatt taactatgct
     9541 tttctttcac ccaatatgca ggtgtggcat cagaaccggg cagaagtgac cttctttaat
     9601 cctaattatt atggaattat ttgttgtttc tgtattatga ttgctttcta tctgtttaca
     9661 acgaccaagt tgaattggtt gaaagtattc tgtgtgattg caggctttgt taatctcttt
     9721 ggtttgaact ttactcaaaa tcgaactgcc tttcctgcta ttatcgctgg agcaattatc
     9781 tatctcttta cgactattaa aaactggaag gccttttggc ttagtattgg ggtcttcgcg
     9841 attggtttga gtttcctctt ttctagtgat ttgggagttc gaatgggtac tttagactct
     9901 tctatggaag aacgcatttc tatctgggat gctgggatgg ccttgtttaa gcaaaatcct
     9961 ttttggggtg aagggccatt gacctatatg cactcttatc ctcggataca tgctccttat
    10021 catgaacatg cccacagtct ttatattgat acgattctga gttacggaat tgtgggtacc
    10081 attttattag ttttgtcttc tgttgctcct gttcgcttga tgatggatat gagtcaggag
    10141 tcggggaaac gtccgattat cggcctttat ctatctttcc ttacagtggt tgctgtgcac
    10201 ggaatttttg acttggctct cttctggatt cagtcaggct ttattttctt gctagttatg
    10261 tgcagtattc cattggagca tcgaatgttg gtatcggaca tgacggatta agttttataa
    10321 gtttgaaatc ttctaccttg ggtaggagat ttttttatac gatgggcatg tcgtgcatct
    10381 gaggtgtaag tcctctgtag ggatccacta ccggtgaata caatagcgct ccccaagcct
    10441 gactatcgtg aggtagcgga ttaaaatggt ctggggatag atcgttttaa gtctgacgca
    10501 ggaaataaga attgtcagag aaagcaatag tgaaatcgta ggctatgaat agaaacgtga
    10561 tagcgaggag catatagtgg ataaggtagc gagaaatttt aaagtataaa agatagtctt
    10621 aatctatata tgtaaatcac aaaagtaatt gaattcggaa taaggttggt gtgaaaagac
    10681 atagtaaact gaatcttgaa tactagaatg atacttctac aatattgtta taatttaatt
    10741 tgaattccaa aatcacattg ttccgtccgt atttcatttt actgtatgtc ttcctaaact
    10801 ccaaagtttc tgcgtcaaat ttcctatttt cactgtagtc tttgacactt cgaacgacaa
    10861 tttgaatcat atattcaaaa aaaaatgaaa aatttgtgcc aacaagccta aatattctga
    10921 aaatttgttt gatttttaat ggataatgtg atataatggt tgtcaatcta taattcacat
    10981 aaacagagga gtagaaaatg aaaaaaaatc gtgtatttgc tacagcaggt cttgttttat
    11041 tagcagcagg tgtacttgca gcatgcagtt cttcaaaatc atctgattca tcagccccta
    11101 aagcttatgg ctatgtttat acagcagacc cagaaacctt ggactacctg atttcaagta
    11161 aaaatagtac aacagtagtg acttcaaatg ggattgatgg tttattcact aacgataatt
    11221 acggtaatct tgctcctgca gttgcagagg attgggaagt ctctaaggat ggtttgacct
    11281 acacttataa gattcgtaaa ggggttaaat ggtttacctc tgatggagaa gaatatgcag
    11341 aggtgacggc taaagatttc gtgaacggtt taaaacacgc agcagataaa aaatcagaag
    11401 ctatgtattt agctgaaaat tcggttaaag gcttggcaga ttatctatca ggaacttcaa
    11461 cagatttttc aacagttggt gtcaaggcgg ttgatgatta tacgttacaa tacactttga
    11521 accagcctga accgttctgg aactctaagt tgacctattc tattttctgg cctctgaatg
    11581 aagaattcga aacatcaaaa ggaagcgatt ttgctaaacc aacagatccg acatccttgc
    11641 tttataatgg tccattcttg ttgaaagggt tgactgcaaa atcttctgta gagtttgtaa
    11701 aaaatgagca atattgggat aaagaaaatg tccacctaga tactatcaat ctagcttact
    11761 atgatggatc agatcaggag tcgctagagc gtaacttcac tagtggagct tatagttatg
    11821 cccgtcttta ccctaccagc tccaactatt ctaaggttgc agaagaatac aaggacaata
    11881 tctattacac acaatcaggc tctgggattg ctggtctggg tgtgaatatt gatcgccaaa
    11941 gttacaacta tacttctaaa actacagatt cagagaaagt agctactaag aaggcattgc
    12001 ttaacaaaga tttccgtcaa gccttgaatt ttgctcttga tcgctcagct tactcagctc
    12061 aaatcaatgg taaagatgga gcagctttag cagttcgtaa tttatttgta aaaccagact
    12121 ttgtttcagc tggtgagaag acctttggtg atttagtcgc tgctcaactt cctgcttatg
    12181 gtgatgagtg gaaaggtgtg aatttagctg atgggcagga tggtttattc aatgctgaca
    12241 aggccaaggc agagtttgcg aaagctaaga aagctttaga agcagacggc gttcagtttc
    12301 ctattcatct ggacgttcca gtagaccaag catcaaaaaa ctacatatct cgtattcagt
    12361 cctttaaaca atctgtagaa acagttcttg gtgttgaaaa tgtcgttgtt gatattcaac
    12421 aaatgacaag tgatgaattc cttaatatta cttactatgc tgccaatgct tcatctgagg
    12481 attgggatgt atcgggagga gtttcatggg ggccagacta tcaagaccca tctacttacc
    12541 tggatatttt aaaaacaact agcagtgaaa ctacaaaaac atatttagga tttgataatc
    12601 caaatagccc ttcagtagtt caagttggtt tgaaagaata cgataaatta gttgatgaag
    12661 ctgccaaaga gacaagcgac ttgaatgtcc gttatgaaaa atatgcagcg gctcaagcat
    12721 ggttgacaga tagttcactc tttattcctg ctatggcttc ttctggtgca gcaccagtgc
    12781 tttcacgaat tgttccattt actggagctt ctgcgcaaac aggctctaag gggtcagatg
    12841 tttacttcaa atatttgaaa ttacaagata aagtggtgac taaggaagag tatgaaaaag
    12901 ctcgtgaaaa atggttgaaa gaaaaagctg aatcaaatga gaaagctcaa aaagaattgg
    12961 caagtcatgt gaagtaaata atttctaata gaactttctc tccatgagga gaaggttctt
    13021 ttgggatttt taaaggaaat aatatgaaaa aatatatttt tatgcgtgtt ttgcggtcat
    13081 tggtttcgat tttcttagta acgactttga cctacacgat tatctatacc ttggttcctc
    13141 gaaaattgat tttcaagcag gatcctaact ataataaaat tgcaacaacg gctgataaac
    13201 gtgataacta tgaaaatact gtgtttgagc gtatgggcta cattgagtat tacgatacta
    13261 aagagttgca agaaaaggca agtagcatgg attcttctgt aacagtagaa gcaaatgcga
    13321 ccaataaagc tatttatgaa aagtacatca atcaattagg tcatggttgg actttgggag
    13381 aatttactga aagtggtcaa ttctatgcta ctcgtgaaat tccaattttt gaacgtgttt
    13441 ttcacttcta tgctaacttg attgacattg accatacaaa taaaatccaa gaccctgaaa
    13501 atccagactt gaaacgctac cttcgttttg aaaatgatcc agctatcgga tggtcattgg
    13561 tcggttcagg aactaaacat aaatatctct tgtactttaa cagtcagttc ccatttgttc
    13621 atcaaaactt tgtgaacttg aatttaggtg actcttaccc aacctatgct aatacaccag
    13681 ttcttcaggt tattactcaa ggtcaaggac aaaccaaaac tgcccaagtt cagttcccaa
    13741 caggtaagaa aacgtcttct gtaaatattt actcaagaac ctacaagtca cctagtcagg
    13801 ctgactctcg tgaagtagct agctatggga aagatgatcc ttatacagcg actgaaagta
    13861 attaccaata tccatctatg attgtcagct ctgctattac tggtttgatt ggtttggttc
    13921 ttgcctatgc tcttgccgtg ccacttggtt cagccatggc tcgtttcaag aacacttgga
    13981 ttgatagcct ctcaacaggg gctttgacct tcttgcttgc tcttccaacg attgccttgg
    14041 tttacatcgt tcgattgatt ggatcatcta ttgcccttcc agattcattc cctatcttgg
    14101 gagctggaga ttggcgttct tacgttttac cagcagtcat ccttggtttg ttgggtgctc
    14161 ctggtacagc catttggatt cgtcgttaca tgattgactt gcaatctcaa gactttgttc
    14221 gtttcgctcg tgcaaaaggt ttgtctgaaa aagaaatttc aaacaaacac atctttaaaa
    14281 atgccatggt tccgctggtt tcaggaattc ctggtgccat cattggggtt atcggtggtg
    14341 caacccttac tgaaacagtc ttcgccttcc caggtatggg taaaatgttg attgactctg
    14401 taaaagcatc taataactct atggtcgttg gtcttgtctt catctttaca tgtatttcta
    14461 tcttctcacg tcttttggga gatatttgga tgactattat tgacccacgt attaaattga
    14521 ctgagaaagg aggcaaataa tgtctacaat cgataaagaa aaatttcagt ttgtaaaacg
    14581 tgacgatttt gcctctgaaa ctattgatgc gccagcatat tcttactgga aatcagtgtt
    14641 taaacaattt atgaagaaaa aatcaactgt agtcatgttg ggaatcttgg tagccatcat
    14701 tttgataagt ttcatctacc caatgttttc taagtttgat ttcaatgatg tcagcaaggt
    14761 aaacgacttt agtattcgtt atatcaagcc aaatgcggag cattggttcg gtactgacag
    14821 taacggtaaa tcgctctttg acggtgtctg gttcggagct cgtaactcca tcctcatttc
    14881 tgtgattgcg acagtgatta acttggttat cggtgttttt gtcggtggta tttggggtat
    14941 ttcaaaatca gttgaccgtg tcatgatgga agtttacaac gtcatctcaa acatcccacc
    15001 tcttttgatt gttattgtct tgacttactc aatcggagct ggattctgga atctgatttt
    15061 tgccatgagc gtaacaacat ggattggtat cgccttcatg atccgtgtgc aaatcttgcg
    15121 ctatcgtgac ttggaataca acttggcatc acgtactttg ggaacaccaa ccttgaagat
    15181 tgttgccaaa aatatcatgc ctcaattggt atctgttatt gtgacaacca tgactcaaat
    15241 gcttccaagc tttatctcat acgaagcctt cttgtctttc ttcggtcttg gattaccgat
    15301 tacagtgcca agtttgggtc gtttgatttc ggattattca caaaacgtaa caaccaatgc
    15361 ttacttgttc tggattccat tgacaaccct tgtcttggta tccttgtccc ttttcgtagt
    15421 tggtcaaaac ttagcggatg ctagtgatcc acgtacacat agataggagt agaaatgaca
    15481 aaagaaaaaa atgtaatttt gactgctcgc gatattgtcg tggaatttga cgttcgtgac
    15541 aaagtattga cagccattcg cggcgtttcc cttgaactcg ttgaaggaga agtattagcc
    15601 ttggtaggtg agtcaggatc aggtaaatct gttttgacaa agaccttcac aggtatgctc
    15661 gaagaaaatg gtcgcattgc ccaaggtagt attgactacc gtggtcagga tttgacagct
    15721 ttatcttctc acaaggattg ggaacaaatt cgtggtgcta agattgcgac tatcttccag
    15781 gacccaatga ctagtttgga ccccattaaa acaattggta gtcagattac agaagttatt
    15841 gtaaaacacc aaggaaaaac agctaaagaa gcgaaagaat tggccattga ctacatgaat
    15901 aaggttggca ttccagacgc agatagacgt tttaatgaat acccattcca atattctgga
    15961 ggaatgcgtc aacgtatcgt tattgctatt gcccttgcct gccgacctga tgtcttgatc
    16021 tgtgatgagc caacaactgc cttggatgta actattcaag ctcagattat tgatttgcta
    16081 aaatctttac aaaacgagta tcatttcaca acaatcttta ttacccacga ccttggtgtg
    16141 gtggcaagta ttgcggataa ggtagcggtt atgtatgcag gagaaatcgt tgagtatgga
    16201 acggttgagg aagtcttcta tgaccctcgc catccatata catggagtct cttgtctagc
    16261 ttgcctcagc ttgctgatga taaaggggat ctttactcaa tcccaggaac acctccgtca
    16321 ctttatactg acctgaaagg ggatgctttt gccttgcgtt ctgactacgc aatgcagatt
    16381 gacttcgaac aaaaagctcc tcaattctca gtatcagaga cacattgggc taaaacttgg
    16441 cttcttcatg aggatgctcc aaaagtagaa aaaccagctg tgattgcaaa tctccatgat
    16501 aagattcgtg aaaaaatggg atttgcccat ctggctgact aggaggaagg aaatgtctga
    16561 aaaattagta gaaatcaaag atttagaaat ttccttcggt gaaggaagta agaagtttgt
    16621 cgcggttaaa aatgctaact tctttatcaa caagggagaa actttctcgc ttgtaggtga
    16681 gtccggtagt gggaaaacaa ctattggtcg tgctatcatc ggtctaaatg atacaagtaa
    16741 tggagatatc atttttgatg gtcaaaagat taatggtaag aaatcgcgtg aacaagctgc
    16801 ggaattgatt cgtcgaatcc agatgatttt ccaagaccct gctgcaagtt tgaatgaacg
    16861 tgcgactgtt gattatatta tttctgaagg tctttacaat caccgtttat ttaaggatga
    16921 agaagaacgt aaagagaaag ttcaaaatat tatccgtgaa gtaggtcttc ttgctgagca
    16981 cttgactcgt taccctcatg aattctcagg cggtcaacgt caacgtatcg gtattgcccg
    17041 tgccttggtc atgcaaccag actttgttat tgcagatgag ccaatttcag ccttggacgt
    17101 ttctgtacgt gcccaagtct tgaacttgct caaaaaattc caaaaagagc tcggcctgac
    17161 ctatctcttc atcgcccatg acttgtcggt tgttcgcttt atttcagatc gtatcgcagt
    17221 tatttacaag ggtgttattg tagaggttgc agaaacagaa gaattgttta acaatccaat
    17281 tcacccatat actcaagcct tgctttcagc ggtaccaatc ccagatccaa tcttggaacg
    17341 taagaaggtc ttgaaggttt acgacccaag tcaacacgac tatgagactg ataagccatc
    17401 tatggtagaa atccgtccag gtcactatgt ttgggcgaac caagccgaat tggcacgtta
    17461 tcaaaaagga ctaaactaat aatggtttta taatttccat gtcaattttt atgggaatta
    17521 taaaactttt ttgttggact gatttgaaaa actttgaaag cttcctgaaa ga
//
LOCUS       DAVBCV010000044        17282 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBCV010000044 DAVBCV010000000
VERSION     DAVBCV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.044"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..692)
                     /locus_tag="VQ516_001485"
     CDS             complement(<1..692)
                     /locus_tag="VQ516_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2511908.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDG"
     gene            complement(803..1057)
                     /locus_tag="VQ516_001486"
     CDS             complement(803..1057)
                     /locus_tag="VQ516_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511909.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(1415..1948)
                     /locus_tag="VQ516_001487"
     CDS             complement(1415..1948)
                     /locus_tag="VQ516_001487"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2511910.1"
                     /translation="MTIFKEQESEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(1935..2261)
                     /locus_tag="VQ516_001488"
     CDS             complement(1935..2261)
                     /locus_tag="VQ516_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2511911.1"
                     /translation="MAYNTDFKQGALDSIKGGHRHVEAAKVFWCWRQNSLHVGKERRE
                     QRNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIAAHFDCAVPSVWAALKQIKVTL
                     KKDDDL"
     gene            2495..4213
                     /locus_tag="VQ516_001489"
     CDS             2495..4213
                     /locus_tag="VQ516_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2511912.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            4324..4671
                     /locus_tag="VQ516_001490"
     CDS             4324..4671
                     /locus_tag="VQ516_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2511913.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(4949..5785)
                     /locus_tag="VQ516_001491"
     CDS             complement(4949..5785)
                     /locus_tag="VQ516_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2511914.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(5798..6427)
                     /locus_tag="VQ516_001492"
     CDS             complement(5798..6427)
                     /locus_tag="VQ516_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2511915.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(6437..7078)
                     /locus_tag="VQ516_001493"
     CDS             complement(6437..7078)
                     /locus_tag="VQ516_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2511916.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(7224..8453)
                     /locus_tag="VQ516_001494"
     CDS             complement(7224..8453)
                     /locus_tag="VQ516_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2511917.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEESENLLT
                     KWMIARSYQEMDDLDTAYEHYQELIGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(8443..9609)
                     /locus_tag="VQ516_001495"
     CDS             complement(8443..9609)
                     /locus_tag="VQ516_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2511918.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            9753..10766
                     /locus_tag="VQ516_001496"
     CDS             9753..10766
                     /locus_tag="VQ516_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2511919.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(10811..11230)
                     /locus_tag="VQ516_001497"
     CDS             complement(10811..11230)
                     /locus_tag="VQ516_001497"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2511920.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDQVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ516_001498"
     CDS             complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ516_001498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2511921.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(12733..13611)
                     /locus_tag="VQ516_001499"
     CDS             complement(12733..13611)
                     /locus_tag="VQ516_001499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2511922.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(13627..15132)
                     /locus_tag="VQ516_001500"
     CDS             complement(13627..15132)
                     /locus_tag="VQ516_001500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2511923.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALIHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(15147..15683)
                     /locus_tag="VQ516_001501"
     CDS             complement(15147..15683)
                     /locus_tag="VQ516_001501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2511924.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ516_001502"
     CDS             complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ516_001502"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2511925.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ516_001503"
     CDS             complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ516_001503"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2511926.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(16942..17142)
                     /locus_tag="VQ516_001504"
     CDS             complement(16942..17142)
                     /locus_tag="VQ516_001504"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2511927.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         5222 a         3786 c         3096 g         5178 t
ORIGIN      
        1 ccgtcagtag tagtcatgct actgagtttt ttatctgtga cattgtagct ggtaccaatc
       61 agttgttttt tattggtaat gtaggttccg tcactttctt tttctccaat tccagtatca
      121 tttttcaatg atagcaaacg cccttgttca tcaacatagc gaactttcac attttctgag
      181 attagttctg ccaattctga ggtttttttc tttttcttga tttcttcggt tattttccct
      241 ttctcttctt cgggaatatt tagttttgga atgatttttt caacaacggt tcgtgatggt
      301 tccacagtat cttggatgac tgaaaagtca gctagaattg ggagattata ctgaacacgg
      361 taactttgag tgtttactcc tactctttca ttattctctg aaaatacttg tacggtataa
      421 gaaacaacat cttttcctaa tagaacatcc ccagtagaga aatagccgcc ttttcctagt
      481 ttgctatctc cagagcccac ttctttccta attttatcag atagtttttt accagtcagt
      541 acattcgttc gcacaatccc tttgtctacc cctacaaagt gggagaactt tttgaactct
      601 tcagaaccag atctagccca accattatta agggcatttg cttttgtatt tgtattctct
      661 ctcaaaggtt tggcgattag aattatattc atcggcactt agagttgctg ctatatctga
      721 ctcttgaata ccaacttcct tactaccatt tctagcggca gtatatgtgt atttatctgt
      781 ttctacttct agctgattat ccttaacatg ataggtaatt ttctcctggt ataagagtct
      841 tcagtagttt atatactctg tcatcatctt tagctttaga agcagctgag tttagccaag
      901 ttggggtatt aggagtatct ttatcaacct gtttcaagac attattcaag tcatttgcta
      961 gcctattggt tatcctcact gtgaaagttt tcttccagtc tgaaccactt ttattagact
     1021 tagatatgct catgaaatcc tggtcaacat tcaacacaat atcgccttgt tttttagcat
     1081 cttccgttgc ttctgcttca aattgatcat ggagtttttg ttgctctggt gtatagtttt
     1141 tttatttaca cgatttaatg tactattttt tattttagta ttaagaactc cattaatacg
     1201 atttttaaac tctgcttcaa aaaatccctt aatttgttgg tgcgcaagtt caacattcaa
     1261 acctttctta tacaagtctt cattctgata caagtcttca ttctgaacga tttcggctac
     1321 acggtgaatg gtttgatcgt ataccgtctt ctcttaacca gcagtatcct tagttttaat
     1381 gactactcga taggttcctt tagaaccgtt atagtcatct agttttaaaa aagcattcca
     1441 aacattcaaa aatagttgga acttccctta ataaatccgt cactttcttt ttcaagatag
     1501 cccaagcttg ctcaatagga ttcaaatccg gtgaataagg tggtagaggt aagaaaaagt
     1561 gtttatcttg gatgcagagt tcatccaaaa tgttcttggg atgaaaacta gcattgccca
     1621 tgacaataac atgaggtgtc ttcaaagcag gtaggagttg cgttttgaac cactccacaa
     1681 agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc tataaactct ccgtctactt
     1741 gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct aatcttttca tagactttct
     1801 cccctctagg agcccctgca taaggacgat agaggtagcg gtcgattcct gtttcatcaa
     1861 tatatacgat tggtaaatct tttaggttat ccaaaatatc aagaaactca gcgactttct
     1921 ctgactcttg ttccttaaag atcgtcatct ttttttaaag tgaccttaat ctgctttaaa
     1981 gctgcccata ctgaaggaac agcacaatca aaatgtgccg caatttcccg taaaaaagca
     2041 tctggatgag cctctacaaa ggctttcaat tcttctaaag ggatcttacg ctttttgacg
     2101 actcgctttt tccgctctaa gttcctttgt tcacgtcttt cttttcccac gtgaagagag
     2161 ttctgacgcc aacaccaaaa aactttggcc gcctcgacat gtctgtgccc tcctttgatg
     2221 gaatctaatg ctccttgttt aaaatcagta ttatatgcca tagctttatt agaccatgct
     2281 tattttaaac taagcaacta tatcaatgtt ttgttatgag aagacttact ttttttactg
     2341 aaattgaatt tttcctagcc tcaacatcat ataaccccaa aattcaatct gacctggttt
     2401 gaagagatgt tcgaagcaga taaggtttta ctaaaactaa aaaaaatgat ataatcaaac
     2461 tgattaaatg ttttaaaaat aaaggagaat ttgaatgtct taccaagaaa attaccagaa
     2521 atgggttgat tttgtggagc ttcctgacta ccttcgtcaa gatttggaaa atatggacga
     2581 aaaaactaag gaagatgcct tctatacaaa tcttgaattt ggtactgcag gtatgcgtgg
     2641 cttggttggt gctggtacaa accgtatcaa catctacgtt gttcgccaag ctactgaagg
     2701 attggctcgt ttgattgagt caaaaggtgg aaacgagaaa gaacgcggtg tagcaattgc
     2761 ctacgatagc cgtcacttct cacctgagtt tgcctttgaa tctgcggcag ttcttgctaa
     2821 acacggcatc aaatcttacg tatttgaaag ccttcgtccg actccagaac tatcatttgc
     2881 agttcgtcat ctcaactgtt tcgcaggtat catggtcaca gccagccaca accctgctcc
     2941 atttaacggt tacaaggttt acggtgaaga cggtggacaa atgcctccac acgatgcgga
     3001 cgctttgact acttatatcc gtgcaatcga aaacccattt gcagttgaag ttgctgatgt
     3061 ggaaactgaa aaagcttctg gcttgattga agttatcggc gaagctgttg acgtagaata
     3121 ccttaaagag gttaaggacg taaacatcaa cccagccttg attgaagaat ttggtaaaga
     3181 catgaagatt gtctacacac cacttcatgg tactggtgaa atgttggctc gtcgtgctct
     3241 tgcccaagca ggatttgact ctgttcaagt tgttgaagcg caagcaactg ctgacccaga
     3301 cttctcaact gtaacatctc caaacccaga aagccaagca gcctttgccc ttgctgaaga
     3361 acttggtcgt caagttggtg cagatgttct tgtcgcaact gacccagacg ctgaccgtgt
     3421 tggtgttgaa gttcttcaaa aagatggtag ctacctcaac ctttcaggta accaaatcgg
     3481 tgctatcatg gctaaataca tcttggaagc tcacaaaaac gctggaactc ttcctgaaaa
     3541 tgccgctctc tgcaaatcta tcgtttcaac tgacttggta acgaagattg ctgaaagcta
     3601 cggcgcaact atgttcaacg tcttgacagg tttcaaattt atcgctgaaa aaatccaaga
     3661 attcgaagaa aaacacaacc acacttacat gatgggattt gaagaaagct tcggttactt
     3721 gattaaacca tttgtacgtg ataaagatgc catccaagcc gttcttgtcg ttgctgaact
     3781 tgctgcctac taccgttctc gtggtttgac acttgctgac ggtatcgaag aaatctataa
     3841 agaatatggc tactacgcag aaaaaacaat ctctgttact ctttcaggtg tcgatggtgc
     3901 tgaacaaatc aaagcgatta tggctaaatt ccgcaacaat gctccaaaag aatggaacgc
     3961 aacagctatc actgtcgtag aagacttcaa ggcacaaact gctactgttg ctgacggtac
     4021 tgttacaaac ttgacaactc ctccaagtga tgtgttgaaa tacacacttg ctgacggttc
     4081 atggattgcc gttcgccctt caggtacaga accaaaaatc aagttctaca ttgcagttgt
     4141 aggagaaacc aacgaagaat cacaagctaa gattgctaac atcgaagcag aaatcaatgc
     4201 atttgtaaaa taaagaccta taaaagatga gaccaaccaa cctcatcttt tttcgtatca
     4261 aatctaagca attttagaaa ctttatggca tactagactt atcaatgaaa gcgaggtaat
     4321 ctcatggaac aatttttaga taacatcaaa gaccttgaag tcactacagt tgtgcgtgcg
     4381 caagaagctc ttgataaaaa agaaactgca accttcttta tcggtcgcaa aacttgccct
     4441 tactgccgta aatttgcagg tacattgtca ggtgtcgtag ctgaaaccaa agctcacatt
     4501 tacttcatca atagtgaaga accaagccaa ctcaatgatt tgcaagcatt ccgctcacgc
     4561 tatggaatcc caactgtacc aggttttgtt catattacag atggacaaat caacgtccgt
     4621 tgcgactctt caatgtcagc acaagaaatc aaagatttcg caggattgta aaaaatcagc
     4681 taacaagtct taaaatcaca aaaacgcata atgtaaggtg caaaaacctt gatattatgc
     4741 gttttattgt tcctaaaaca gttacatcag catttcatta cagaacattt taattttgga
     4801 agtcaaatca atttttaaca atgttttaga aatcacagtg ggctattcca tattaaatta
     4861 tccagattca atcgctatta tcgttttcct agattacaaa aaaactgcgt tttgctaatc
     4921 aaaacgtagt ctcagccact attttatctt actgtccttc ttttacttct ttggttgcta
     4981 catcttctcc aaaccatttt tggctgattt cttggaactt gccgtccttg taaagactag
     5041 aaaaagcttc atttatcttc ttaaccaagg ttgtatcttc cttacgggct ccaaccgcaa
     5101 aagcttctgt ttctagtcca actgtaaaga cattataatc gtttaaaaca ccttctgctt
     5161 ctaaataata gtttgcatag acacggtcaa tcaatagacc atcaattcga tcgtttttca
     5221 aatcaatcaa ggcttcatta aaggtttggt attgattcgc ttccttatta gcgacaatat
     5281 tcttcaaaat ttctggattt gcttcaaagt ccgcataacc agatgaacca gcttgagctc
     5341 ctaatgtctt tccagtcata tcctttgcag tcgtgatacc agatgatttc ttcgtaacca
     5401 atacctgctc attcttcata tatgagttac tgaaagccac cttttcacgg cgttcgtctg
     5461 tagcggaata gccattccaa atcagatcaa tcgttccttt tgtcaattca gcttctttca
     5521 aatcccaatc aatcggttgc caatttaccg tgattccgta tttttcaaaa acagctgtag
     5581 ctaaatcaat atcaaatcct gcataagaac catctttctg agcaaatccc attggaacaa
     5641 aagtactatc aaatccaata gtaatagact tgttagactg gtactttgac caattatctc
     5701 cactagtttc gctagaattt ttcccacaag ctactaagaa caaagcagtc atcagactga
     5761 ctaatacaag catccatttt ttcatccatt tttcctccta tttaggttct actttcaata
     5821 atacatctgc gatattttca gcaaactgca aatcatgggt aaccacaatc tgggtcatcc
     5881 caagttccct attttgcaag attagcttct ccacttccaa acgtaattct ggatccaggg
     5941 cagaagttgg ttcatcgtag ccaatgattt ctgggtcaat catcatagca cgcgccaaag
     6001 ccacccgctg cttttgccca ccagatagtg agaaaggata ggcctctgcg tgtcctccta
     6061 gtcctaactg ttccaagagt ccactcgcct tcttctcagc ctcttcctgc ttcattccca
     6121 tggtcttcac aggcgataag gtcaaattgt ccagaactga taaatgaggg aacagttgaa
     6181 aatcttggaa tacaaatccc agtagattgc gcttctgcaa ttcatccagc tctaaaggtt
     6241 gtccattata aaagatttgc cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa
     6301 gagttgtctt acctccacca gaaggtccaa cgatagccag gatttgcttt tcaggaatac
     6361 ttagactgaa attagacagg atttgtttgt ctccaaagac tttattgata tttcgtaatt
     6421 ctaacatggc agcctcctat ctataataac tatacttctt ctcaactttt ttggaaataa
     6481 ttgtcacaat cccaatcaaa atcaaataaa tggctcctgc caagaacata ggaactagac
     6541 tagcatcgcg gttagcagct gttcgactag ccaagataag gtctgaaatt ccgagagcat
     6601 agaccaaaga agtatccttg accaaactca taacttcatt aaagacacta ggaagaacga
     6661 tcttggtcac ttggggcaag ataatatagc gcactctgtc aaaagggcta aacttcaaga
     6721 ccttggcggc ctcatactgt cctcttggaa tagtgtcaat tcccccacgg aaaatttctg
     6781 caaagtaagc tgcatagttg agaacaaagg caataatagc tgcaggaagg cggtctaaac
     6841 gaatcccaat acttgggagc acataataga taaaaatcag ttgcaagagt aagggggttc
     6901 ctcgcataac ccagatgtaa atgttaatca gataatggag gggcttccaa tggacttgca
     6961 aggcaaaggc aatcaaaacg cccaagggaa tcgaaaatag caagaccagt gcaaagacct
     7021 gtacagtcgt gctcgcacca ttcagtaaac tcggtaatat ctcaaataaa taagacatac
     7081 tgcacctcct aaaagtaatt gttcctatta tagcatgaat aaacaagatt accaagtatt
     7141 tttgtaaaaa gattctacca tatacaaata aaaaccgtag ctatcaaaag agataaacta
     7201 cagttctata tgtttaaaca ttcttacaat ctctcaaaca gttcttgcat ttgcacatca
     7261 tctggaacca gttttaagta agtgtgagca tggacttttg cttcttcaaa atgtcccaat
     7321 tcacgcaaga gatagatata gtgttccaga aattctggat tgtccttcaa atctcctatc
     7381 aactcttgat aatgctcata agcagtatcc aaatcgtcca tttcttgata agaacgagca
     7441 atcatccact tggtcaaaag attttctgac tcctcactct gcaagtctag aatatcctca
     7501 taacgctcct gctccagata aatagtggct aaacgaagca agatttcttc tgtatcctca
     7561 gcgtcttctt ttgcagtaag gagataattt tctgcaccac tagcatcatg caattcataa
     7621 gaaaattgtg aagcagctag caagaggcga gtttcaaagg gatttttctc taatccttgc
     7681 ttagcgatac gcagggcttc ttgaacttga tgttccttat gtaaagcctg actgtaccca
     7741 tactcatagc cttcaaagtc aggagaaatg gtatcaagct gcttaaagta gagggtggct
     7801 ttttgatatt cttcttgatc aaaataaaga ctggccaact caaaagctgt taagtcatcg
     7861 tattctaact ccagggcttt ttctaaaaac tcagtagccg tttcaaattt ccctaactga
     7921 gcataggcaa agccaattcg ttgataggtg gaaatgcccg tttgctcata aatcgagcga
     7981 ttatctaact gggcataggc ttgaatagcc gcttggtaat tttccaactc actatccaac
     8041 tctgccaaac ccaatatcaa gagagaatcc tctgagtagg tcaaggcctc caataatttc
     8101 tcacgtgcca catctgtcaa accttccagc tggtaaaggt ctgccttcag agccaaagac
     8161 gagacatacc agtcactgtc agcttggatt tcctcaagat aggtaaaggc ttcttctatt
     8221 tgaccatcct cgctagcaat tgcagctaga ttaagatgaa cctctggaaa atcctctaca
     8281 attttcaggt aaatttcctt ggcctgagga tagaaaccaa tcccttcaag ataagttgcc
     8341 aactcataca gaagatcact tgaatcattt tctaaagctt tggcgaaata atgctcagcc
     8401 ttagttaaat cttgctcctc caaagcctgt aacatctgtt gactattgtt cactcttgac
     8461 ttcctcaccc tctaattcat atagaccact gactacctta taccattcga aaatggctga
     8521 aatgacaacc ttagcagagg cataaaccgg aataccaagt aaaactcccc agataccaaa
     8581 catagatcct gaagttaaca aaacaaagag aacattaata gggtggatgt ttaattgact
     8641 tcccaaaatc aatggagaga caaaacggcc ttcaatagtt tgttctacga taaagacaat
     8701 cactactttc aaaagcatga ctggaccagc aatcaaaccc aatactagag caggaagcat
     8761 ggctagaaag ctaccaagat aagggaccag atttaaaata ccagcagtaa cccccagcgt
     8821 aaccgcatag cgtagaccaa taatcttgaa gaagatgata aacattactg ctacaataat
     8881 agccactgtc acttgccctc gaacatagtt ggacaactgt tgattcacat ctgataaaac
     8941 ttgtccaaca ggttccttca attttcttgg aatgaattgg gtcaaatagt tacgcaagcc
     9001 tttcccatca cgcaagagat aaaagagcat gaaaggaacg ataatcaagg caacaatcac
     9061 ttgagaagct ccgctaataa aggcactcac ccagttgact gcctgagatg aaaccttact
     9121 tgccaaaact gtagcctggc tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa
     9181 atcatctggc aggtgctggg ctaccaatcc attaacaatc ctatctatat cttctaagta
     9241 aacaggaacg tttcttgcaa aggtcaaaac ctgacgttgc agatttggaa tggcgactgc
     9301 caagccccaa atgataaaga gagcgatgat aacaaagaca atagtgatag ctataacacg
     9361 attaacctta tgcttctcca tccaatcaac aataggattc aacaaataat ataacaaacc
     9421 agacaaaatg actggcaaca tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa
     9481 actaatctta cttaaaataa aaagattcag tcccaataat aaggttacta aaaataccgt
     9541 aattgccttg ttatctaaaa accacttgaa aaaccaagat aggctaaaat gtttctcttt
     9601 ttgctccata aatacttcct ttctattgtt ctctcattat accatttttg aaacaaaata
     9661 actctttttt aaagtgctta catctaattg gatatcttta atttacttgt ttttgtgata
     9721 taatagtctt atctttacac agaggtatag taatgaaaga aactgtttat tttggaactt
     9781 atacacgtcg tacttctcaa gggatttaca aggcagactt tgatacagaa actggtcagc
     9841 tttcaaatct agaacttttt gcagctgagc caagtccaac ctaccttgcc tttgaccagc
     9901 accaacattt atacactgtt ggtagccaag acgataaggg gggaattgca gcctatcaaa
     9961 ctgacgggac tgtgttaaat catgttgttg aagaaggagc tccccactgt tatgttgctg
    10021 tcgatgaaaa gcgtgatttg gtttacgcag ccaactatca caagggacaa gtccttgttt
    10081 ataaacgcca ggaagatggt agtcttctac ttagtgatat ggatcaacac agtggccaag
    10141 gtccacatga aaatcaagct tccccccatg ttcactatac agatttaaca cctgaccact
    10201 atctagtgac ctgcgacttg ggaactgacc aagtcatcac ctatgacctc gatcaagaag
    10261 gaaaattatc taagctctat acctatcaca gcaagccagg agcaggctca cgccatatca
    10321 ttttccataa ccactataaa atcgcttatc tcatttgtga actcaatagt actatcgaag
    10381 ttttaatcta cgatggcgtt ggcgaatttg aacgtatgca ggtcatttca actttaccgg
    10441 aagcttacga aggctttaat ggaaccgctg ctattcatct ctctaaagac ggtaaatacc
    10501 tctacgcttc taaccgtggc catgattcta tcgcagtata taccatcctt gcggacggta
    10561 gcttagagtt attagaaatc gttccaacgc atggtcagac tccacgtgat tttgatttga
    10621 cacccgacca aaaatttctc attgttgtcc atcaagactc tgacaatgca actgtcttta
    10681 aacgtaattg tgacaatggt cgtctagcag aactctccaa cgacttccat gttcccgaag
    10741 cagtctgcat ccgttttgct ccttaaaaca ccataaaaaa tgaacttggt attttcaagt
    10801 tcattttttc ttatagtcta tttccgacat taatacggtt aatagcacgt tgcaaagcaa
    10861 tcttagcacg acgttcttgg tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt
    10921 ctgcacgaag tttggcacgt tctgcacgac tgatatcgat atcacgagca cgttctgcag
    10981 agtcagcgac gattgtgatc atatcattgg caatttcaat aacaccaccg tttactgcaa
    11041 tccagttcac ttgatcttta tcatcgatac gttttacctt tacttcatca actgctaaaa
    11101 ccgcaatcat attttcatgt cgtggcaaga tccccatctc accatccaga gttcgaaccg
    11161 atacatagct ggcatggtga tcatagacga gaccatctgg tgtcacgatc tggacagtta
    11221 actgagccat agttcacctc ttaaaatccc attttttcag cttttgcaat cacatcttcg
    11281 atagaaccta caccacggaa ggcatcttct ggcaagtggt cgtatttacc atcaaggatt
    11341 tccttaaagc cacgtacagt ttcagcaact ggaacataag aacctggctg accagtaaat
    11401 tgttccgcaa cattgaagtt ttgtgacaag aagaactgga tacgacgggc gcgagcaacc
    11461 aaggtctttt cttcatcaga aagttcatcc ataccaagga tagcaatgat atcttgcaat
    11521 tcatggtaac gttgaaggac acgttttact tcagcagcaa ctgcatagtg ctcttctcca
    11581 acgatttcag gtgccaaggc acgtgagctt gaagcaagtg ggtcaacggc tgggtagata
    11641 cccaattgta ccaacttacg ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt
    11701 gctggcgctg ggtcagtata gtcatccgct ggcacataga tagcctggat agaggttaca
    11761 gaacccttct tggttgatgt gatacgttct tgcaattgac ccatttccgt agcaagtgtt
    11821 ggttggtaac caacggctga tggcatacga cccaaaaggg cagatacttc tgaaccagcc
    11881 tgagtgaaac ggaagatatt atcgataaag agaagcacgt cttggccttc cacatcacgg
    11941 aagtattcag cgattgtcaa accagtaagg gcaacacgca tacgtgctcc tggtggctca
    12001 ttcatctgac caaagaccat ggctgttttc tcgataacgc ctgattcttt catttcccag
    12061 taaaggtcat tcccctcacg agtacgttcc ccaacaccag caaatactga aataccaccg
    12121 tgctcttggg caatgttgtg aatcaattct tggattaaga cagttttacc aactccggca
    12181 ccaccgaaaa gtccaacttt accaccttta aggtaagggg caagaaggtc aataaccttg
    12241 atccctgttt caaggatttc agaagaggta gacaactcat caaaagttgg agctttttta
    12301 tgaattggct gacgctctgc gtcttctgta aaaggagctt ccaagtcaat ggtatctccc
    12361 aaaacgttga agacacgtcc caaagtttct ttacctactg gtacagagat tggacgacct
    12421 gtgtccaata cttccattcc acgagtcaac ccatctgttg attccatggc gatagtacga
    12481 accataccat ctcctaactc caaggctact tcaaggacga tttttgtttt tctttcgtca
    12541 tttttgtaga cgacaagtgc attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa
    12601 acgtctacaa cgggaccgat aacctgagca attttacctg aactcatctc cttctcctat
    12661 tctatataag atactgtcgg ttcctagctc aactaggtcc taagttcatt ttcatacgag
    12721 ctggactaga gcctattcta aggcactagc acctgctacg atttctgtaa tttcttgtgt
    12781 aatcgccgcc tgtctggcac ggttatactg aattgtcaaa tcattgatga ctttcttagc
    12841 attatctgtc gctgtttgca tggctgtcat acccgcagca ttctcagctg tcttggcatc
    12901 tataatggca ccgtaaatca tactttctgc aaactgaggc aacaactgct ccagaatttc
    12961 ttctcggctg gtttccaatt caaaagtcaa gctgtactct tcatccgctt catttggatc
    13021 caagtcaaca atcggaagca tttgttccac acgcatttga ctggttagcg tattgacatg
    13081 gtggttgtag caaacataaa gctcatcaaa gagttcattt tggtacattt caacagtttt
    13141 tgaaataatc ttacgaactt gatcaaagct aggttggtct gccaagccac gtaattcata
    13201 aagtggttga ataccgcgag ccttaaagaa atcagctccc atcccaccga tacagatcat
    13261 ttcaaaacct ttaccgtctg ggtggtattc ttctttcaac tccataacag ctttcaaaat
    13321 agaggaatta taacctccaa ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt
    13381 cttcacagaa cggctaatca acatcggatt agttgaagca ccagctccat taccatgaag
    13441 gatatctgtc aaaagtttac gcactttctg agcgtaaact tggaagttgc gagcagcttc
    13501 ttcagagcga cctagcttag cagccgatac catttgcatg gcattagtga tttgactcgt
    13561 attttttgtt gaggcgattt ttgttttaat atcatttaga gatactgcca tctgacacct
    13621 ctattcttat tggaagctag attgattgag aaactctgta atcgcagcat ccaagactgc
    13681 ttcttctggc aagtcttttg tatcacgaat ggtttccaaa atctctggat gttgagcatc
    13741 aaagaaggca tggaactctt cctcgaaacg aacaatatca tctactggaa cagtatccaa
    13801 gaaaccatgt atcaaagcat aaagaatggt tacttgtttc tcaacaggta atggtttgtg
    13861 aacaggttgt ttcaagacct caacggtacg acgtccacgg ttcaacttag cctgtgttgc
    13921 tgcgtccaag tcagaaccaa acttagtaaa ggcttccaac tcacggtatg aagcaaggtc
    13981 gatacgaagt gtaccagcaa ccttcttcat ggctttgatt tgtgcagaac cacctacacg
    14041 agatacagat gaacccgcat cgatggctgg acgaatacct gcattgaaga ggccatcgcc
    14101 aaggaagatt tgtccatcag tgatagaaat cacgttggtt gcgatatagg ctgagatatc
    14161 tcctgcttgt gtctcgataa atggtagggc tgtaattgat ccaccaccaa gttcatcaga
    14221 aactttagct gagcgctcaa gcaaacggct gtggagatag aaaacatccc ctgggaaggc
    14281 ttcacgacct ggaggacgac gaagcaagag cgacagttca cgataagcta ccgcttgttt
    14341 tgatagatca tcgtatacaa tcaaaacatg cttaccttga tacataaatt cttccgccat
    14401 agcgacccca gtataaggag ctaggaagag caatggagat ggttgtgaag cagaggctgt
    14461 cacaacgatt gtgtagtcca aggcaccgta ctgacgaagt gtttctactt gcgtacgaac
    14521 tgttgattct ttttgtccaa tcgcgacgta gatacagatc atatcttgat ctttttggtt
    14581 caagattgta tcaatcgcaa tggttgtttt ccctgtctga cggtcaccga taatcaactc
    14641 acgttgacca cgaccaatcg gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg
    14701 ttctgaaaca gacttacgtt gcataacacc aggagctggt gcttctactg gacgagtttt
    14761 atcagtgtgg atttctccaa gaccgtcaac tggacgacca agcggatcca caacacgacc
    14821 aatcagactt tcacctacag ggacttccat gattttccct gtacggcgga ttgtatcgcc
    14881 ttcacggata tctgtaaagt cacctaggat gataatacca acgtctgttg actccaagtt
    14941 ttgagccata ccataagagc cgttttcaaa attcaacaac tctccactca tgacattttc
    15001 aaggccgtga gcacgcgcga taccgtcccc gatataggtt acaacacctg tttcagtcac
    15061 atcaaaattg ggtttgaaat tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc
    15121 gttaattgcc aaaagaacac cactttctat ttcaaatttt ctttaacaac tttaagttgt
    15181 tgtttaatac tcacatcaat tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga
    15241 ctttcatcga tttgttcttt tacactcctt actttcagag acattttttt ctcaatcaaa
    15301 gggagcaaac gagtcttctg ttcatcagtt agaggatgag cagacgtaat cgtcacttca
    15361 aatcgatttg tttctttttc aagtcggttc aagcaatcta caagcacatc ataaaaaaga
    15421 tttgctctgt gattgtaggc cagaacctgg ataaagtttt gtaataaagg cgacacagaa
    15481 tcttggaaaa aagcaattgt tttttcctta tccgactcgt ctactgccac ttgttttaaa
    15541 aaagaaggca gacctgtttt ttcaacaact tgcttgattt gagtcaagtc tgaaaagata
    15601 cggtcttctt ctcctttttc aagtaccaat tggacaaaag gcatgctgta tttttcaatt
    15661 acctttactg ttttcttgtc cattaagctt ctcctagctg atcgatatac tgatcaatga
    15721 gtgctttatg ggcatgactg tcaaggtttt gtgagatgat tttaccagct aagctgatgg
    15781 tcagatctgc gacctcaccc ttaacactct gtaaagcttc tactttattt tgagcaattt
    15841 cttgattggc tttttctttt aagtgtcctg cttctagttt agcatctgct aagatattag
    15901 ccttactttg ctcagctgtt tcctttgcat tttcaatgat tgtcttagct tctttacggc
    15961 taccagccaa ttcatcttcg cgtttttgag ccaatacttc tgctttttga cgggcttctt
    16021 cagctctgtc aatatctgaa gcaatttttt cagctctttc ttcgaaaatg cctgtaatat
    16081 tagaccatgc aaatttttta atcaagacta gcaaaagaat aaaagagcca gtgattaaaa
    16141 taaaattacc aattaattca cctactgtta cgtgcatcag ttactccttt ctattcctct
    16201 tcaatattaa tcttattccc taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg
    16261 atgcaggaaa taaagacaga aaatgcagtc caagccaaat tagctccaaa ggctactgga
    16321 taccaataaa tagcttggtg ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca
    16381 aagatattcc caaaaatccg caaagccaaa gataagaagt ttgtaaattc ttccaagata
    16441 ttcatcggta tgacaaaaac aggactcata aaagatttta tacttttttt aaatccacga
    16501 cgacgaacgc tttctatatg tgtcaacaaa atgacaagaa aagataaggt taagtcatac
    16561 tgtaaattag cggttggcga actccaccag ttagtcccat cgatcgtttg aagctttgtc
    16621 attaagccaa ggttattggc aatcaccatg aaaaggaata aacaaaggaa aaagagtgaa
    16681 taatctttca tgtagcgcga accaatgtta ggttctgtaa atccaataac aaagtcatag
    16741 acatactcaa gtacattttg ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa
    16801 taaataaaga caaaaataac tcccacaatc aacaaagtca tggctaacat agtcagattg
    16861 aagataacag gaccaataga gatgattgga ttaatacttt cttccatcta aaatcctccc
    16921 ttttcttctc gttccatgta tttatttgat aataaatgag aagacaagag ttacaaagaa
    16981 agttccttca ataaaggcaa cacctaaaaa catcaaacta cgaaactcag aaagcatatc
    17041 tggttggcgt gctactgatt taaacagtcc attcatcaat aaaccttcac cgacagatac
    17101 gcccatacag gcaatacata agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa
    17161 ataaaattta cttacttagt gtagtcctaa aatttaaatt tgtcaacttt taaccgctat
    17221 tggaagcgtt taataaaaat tatttccatt ttgctaaatt tcaagttcaa gttagccatc
    17281 ca
//
LOCUS       DAVBCV010000045        16132 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBCV010000045 DAVBCV010000000
VERSION     DAVBCV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.045"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            145..6831
                     /locus_tag="VQ516_001505"
     CDS             145..6831
                     /locus_tag="VQ516_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2511928.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTVEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQTSNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGFKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEDTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQIRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGKERIFT
                     AINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEASQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7079..7935)
                     /locus_tag="VQ516_001506"
                     /pseudo
     CDS             complement(7079..7935)
                     /locus_tag="VQ516_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8146..8505)
                     /locus_tag="VQ516_001507"
     CDS             complement(8146..8505)
                     /locus_tag="VQ516_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2511929.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8597..9553
                     /locus_tag="VQ516_001508"
                     /pseudo
     CDS             8597..9553
                     /locus_tag="VQ516_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9537..10094
                     /locus_tag="VQ516_001509"
     CDS             9537..10094
                     /locus_tag="VQ516_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2511930.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10087..10341
                     /locus_tag="VQ516_001510"
     CDS             10087..10341
                     /locus_tag="VQ516_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511931.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10353..12407
                     /locus_tag="VQ516_001511"
     CDS             10353..12407
                     /locus_tag="VQ516_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2511932.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12586..13446
                     /locus_tag="VQ516_001512"
     CDS             12586..13446
                     /locus_tag="VQ516_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2511933.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13657..14367
                     /gene="ccdA2"
                     /locus_tag="VQ516_001513"
     CDS             13657..14367
                     /gene="ccdA2"
                     /locus_tag="VQ516_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2511934.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14387..14953
                     /locus_tag="VQ516_001514"
     CDS             14387..14953
                     /locus_tag="VQ516_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2511935.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14964..16076
                     /gene="msrB"
                     /locus_tag="VQ516_001515"
     CDS             14964..16076
                     /gene="msrB"
                     /locus_tag="VQ516_001515"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2511936.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         4901 a         3052 c         3565 g         4614 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgttgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caaacttcaa atcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ttttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgtagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt attcttgaat ggaaaatctc ttggttttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     3181 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     3241 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     3301 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaggca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ctgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggtcc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgac aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaaga tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 ataagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcc
     5821 caagccaaac ttcgtttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
     6421 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
     6481 tatctaggtg aaactcgtgt agaacaagaa ggaaaaactg gtaaagaacg tatctttaca
     6541 gcgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agctccgaca
     6601 gaccgcatag tcttggttgg aaccaaacca gtagctcaag aagctaaaaa accacaagtg
     6661 tcagaaaaag cagatacaaa accaattgat tcaagtgaag ctagtcaaac taataaagcc
     6721 cagttaccaa atacaggtag tgcggcaagc caagcagcag tagcagcagg tttagctctt
     6781 ctaggtttga gtgcaggatt agtagttact aaaggtaaaa aagaagacta gaaattttga
     6841 taccttcttt atcattaaag accaaagaaa aactcttgtc ctaaatagaa gataaagagt
     6901 ctggaacaaa atagttctca accacaaaaa agctagatat ttccaattgc ggaactctag
     6961 ctttttaatt ttgagtcgtt ctcttattgt atagtagatt gaaactagaa tagtacactt
     7021 ctacttctaa aacattgtta gaaatcaatt tgactgtcct tatcgatttg tcctgttctt
     7081 atttcatttc actatgtttt aggaggttat tggccataag taccaatccc atgtcaattc
     7141 tcacttgacg cttccctctc agattacatc tcttgtaacc caaacaagcc tttatctgcc
     7201 caaagacagg ttccacatca atcttgcgtt gagcgaaaat ctgtctacct tggggagata
     7261 aaagcgcctg acattcttta gctttcaagt tttgatagcg ttcgttcata tacagtccct
     7321 tttgagggac tgattcaggt tcatcagcgt agtaaacctt gatttcctgt tgaaagtctg
     7381 tctgtgtttt ctggtacttg atatggtgaa aacgatagca ccagccatca ggatgtgtgt
     7441 agctatcctc cttgtcatta tagtgccaat tcgctaagtt tttagctgac tgtttatacc
     7501 ctctcttttg ttccttatca aacatggcat atttaatcag aaggggagtg atttcttctt
     7561 gaaaatagac ctgtatctgt tcttgaagtt tggcggagaa tttctctgtc gttttcttcc
     7621 acacaaaact atacttgttg gcattggctt caatcttagt tccgtcaata aacaaacaat
     7681 ctaaggtcac taactcttcc atttttcaat ttttcgacca gagaaaatcc cttgtgaata
     7741 ggcaaataga agagtagata caagcatttt agggtgataa gacgggcgat caaaggcatg
     7801 atagaaggcg tggaagtgac aatcctccaa ggtattcacc actttttcaa tagtaaaaac
     7861 gagatgatct tgtggcaaga aggaactgat ttctagtggt aaagttgttt gatttgtgtt
     7921 atagtgaata tgcatgggat agcctttcta aatggtttat taagcaattc tattttacca
     7981 agactatcgc aaccatgcaa aaaagcaacg gcaaatccgt tgcctttttt ggagataaga
     8041 gactattgtc ccagactctt ttctttgtat aaaattgtat agtgttattg tctatactat
     8101 tttgtagaga aagcaaaaat ccctagaaaa tgcttaggga ttcgactatt tcattaagaa
     8161 caattcaaca atcatttcta tatacatgat aagggttaat aagaatcctg cacgccagaa
     8221 gaatttgata aatttaggat agtaaaagct gcgttttttc aaaaggaaga aaaagacgag
     8281 aataatggct aggatcgaga gggctagccc cagtctaggg aggaaattat gggtaaaggt
     8341 tttagctgta atcaagtaat actcaaatac caagactgga aaagccaaat ccgcaaagtt
     8401 tcgtcctagt ttttttaatc taaaaagttt ggttaggata atgcagacga ctaaggtcag
     8461 tatcaataat aaaatagatg ctaatttcat taaaatcata cccatattgt atcataaaaa
     8521 aaggctaatg gaaacgagaa acagaggaat ttataggaaa gtcaggcttt tgagattgtg
     8581 ggtgattttt gttataatga aagttataaa atcttataat accttgaatg attattatcg
     8641 aaaactcttt ggagaaaaga cttttaaagt ccctattgat gcgggatttg actgtcccaa
     8701 tcgtgatgga actgtggctc atggaggctg tactttttgt acggtttccg gttctggaga
     8761 tgccattgtg gcaccagatg cgcctatccg tgagcaattt tataaggaaa ttgactttat
     8821 tcatcgtaag tggccagatg ttcagaagta tctggtttat tttcaaaatt ttaccaacac
     8881 ccatgaaaag gtggaagtta ttcgagagcg ttatgaacag gctatcaatg agccaggtgt
     8941 ggtaggaatc aatattggaa cgcgcccaga ctgtttacca gacgaaacca tcgaatattt
     9001 ggctgagtta tcggagtgca tgcatgtgac ggttgaattg ggcttgcaaa ctacttatga
     9061 agcaacctct gacctgatta accgtgcgca ttcttatgaa ttgtaggtgg aaacggtcaa
     9121 gcgtttgaga aagtatccaa aaattgagat tgtttcccat ttgatcaatg gtttgcctgg
     9181 tgaaacccat gagatgatgg ttgaaaatgt ccgccgttgt gtcacggata acgatattca
     9241 agggattaaa ctgcatttgc tccatcttat gacaaatact cgtatgcaac gagattacca
     9301 tgaggggcgt ttacaattga tgagtcagga cgaatatgtc agggtcatct gtgaccaact
     9361 ggaaattatt cccaaacata tcgtcatcca tcgaattaca ggagatgcgc ctagggatat
     9421 gctgcttggg cctatgtgga gcctcaataa atgggaagct ctcaacagca ttgagatgga
     9481 gatgcgacgt cgtggaagtg ttcaaggatg caaggctgta aaacaggagt ttgaaaatga
     9541 aaagaccact tgagatggca catgattttt tggctgaggt cgtgacaaaa gaggatgtcg
     9601 tagtggatgc gactatggga aatggtcatg acacgctttt tttagccaag ctagccaagc
     9661 aagtctatgc ctttgatatt cagaagcaag ccttggaaaa gacccaagag cgtttgcatc
     9721 aggctgactt gacaaatgcc cagttaatct tgcaaggcca tgagacactg gaccagtttg
     9781 tgataaaagc taaggcaggg atttttaatc tgggctattt gccgtcagct gataagtctg
     9841 tcatcacccg accgcagaca acgattgagg cattagaaaa gctatgtggc ttacttgtca
     9901 aaggtggacg aattgctatt atgatttact atggtcatga aggaggcgat ctcgagagag
     9961 atgctgtctt ggattttgtg atccagttga accaacaaga gtacacagct gccatttacc
    10021 gaactttaaa ccaagtcaac aacccgccgt ttttagtgat gattgaaaaa ttagagagat
    10081 acagacatgg ataaacaata cctacacgaa aagctggatg ccatgcgcca gaattttgtt
    10141 gaatcaaccc accacgaacg atcggtgggc gtgctagacc aagcgcatat gagcaaaaaa
    10201 atgcttaaaa tcaagaaaaa attagttgct cttgaaatgg aacggtgcca gagaaaaatt
    10261 gagcacaaag actgttccaa gattgaccaa aaaatcaaag agcagaagga gatatttgaa
    10321 tcctgttgta aaaaagatta aggaggacgt gcgtggaatt actgatttac ctcatcctat
    10381 ttttactggt tttgattgtc tcaagtacaa ccaataagct cctgcccttt ttgcctctcc
    10441 ctttggtgca gattcttttg ggaattgtga ttggtctctt tttacccaat actgactttc
    10501 accttaatac ggagttgttt ttggcactgg ttatcggacc cttgcttttc cgagaggctg
    10561 aagaagcaga tgttacggct attttaaaac actggcgaat cattgtttat ctcatatttc
    10621 cagtgatttt tatctcgacc ttgagtttgg gtggcttggc ccatcttctt tggttcagcc
    10681 ttcccttggc agcttgcttg gctgttgggg cagcccttgg tcctacggac ttggtggcct
    10741 ttgcctctct ttcggagcgt tttagctttc ctaagcgcgt gtccaatatt cttaagggcg
    10801 aaggactctt gaatgatgct tctggtttgg tggcttttca ggtagctttg acagcttgga
    10861 caactggagc tttttctctg gggcaagcta gcagttcgct catcttttca atcctaggcg
    10921 gttttttaat tggattttta acggccatga ccaaccgctt cctccatacc ttcttgctaa
    10981 gtgtgcgcgc aacggatatt gccagtgaac ttttattaga attgagtttg cctctagtga
    11041 ccttctttct ggcagaagaa gttcatgttt caggtattat tgccgtcgta gttgctggaa
    11101 ttttaaaggc aagtcgcttc aagaaaatca cgctcctcga agcccaagtg gatacggtga
    11161 ccgagacggt ctggcataca gtgaccttta tgctcaacgg ttctgtcttt gtgattttag
    11221 ggatggagtt ggaaatgata gcagaaccta tcttgaccaa tccaatctat aatcctctac
    11281 ttttattgct atctctcatc gcccttacct ttgtcctctt tgtcattcgt tttattatga
    11341 tctatggcta ttatgcctat agaacccgac gcctcaagaa aaagctaaat aagtatatga
    11401 aggacatgtt tctcttgacc ttttcaggtg ttaagggaac ggtgtcgatt gctacgattc
    11461 tcttgatacc aagtaatcta gaacaggagt atcctctttt gcttttcctt gttgcaggtg
    11521 tgacgcttgt cagcttttta acaggtctct tggtcttgcc tcatctttct gatgaagagg
    11581 aagaaagcaa ggattatctc atgcatatcg ccattttgaa tgaagtaacg ctagagttgg
    11641 aaaaagagtt ggaagacacc agaaataaac ttcccctcta tgcggctatt gacaattatc
    11701 atggacgtat tgaaaatctc attttaagcc aagaaaacca ggatgatcaa gaagattggg
    11761 ctgctttgaa actcttgatt cttagtattg aaagtgatgg tttggaacag gcctatgaag
    11821 aggggaacat tagcaatcgt gcttaccgag tttaccaacg ttatctgaaa aatatagaac
    11881 aaggaatcaa tcgtaaactt gcctcaagac tgacctatta ttttcttgtt tccttgagga
    11941 ttttacgttt tctccttcat gaagttttta ctcttggaaa gaccttccgt agctggaagg
    12001 acaaggagca aagccgtctc cgtgctcttg attatgacca aattgcagag ctctatcttg
    12061 ccaatacaga gatgattatt gaaagtttgg aaaacctgaa gggagtctac agacgctctt
    12121 tgattagttt tatgcaggag tctcgtcttc gagaaacagc tattatcagc agtggtgcct
    12181 ttgtcgaacg ggttatcaat cgtgtcaaac ccaacaatat cgatgaaatg ctgagaggct
    12241 attatctgga gcgcaagttg attttcgaat acgaagaaaa acgattgatt acgactaagt
    12301 atgccaagaa attacgacaa aatgtaaata acttagagaa ctattccttg aaggaagctg
    12361 ccaataccct gccgtatgat atggtggaat tggtaagaag aaattagtta atactcttcg
    12421 aaaatctctt caaaccacgt cagcgtcgcc ttggattata tatgtgactg acttcgtcag
    12481 tttcatctac aacctcaaag cagggctttg agcaacctgc ggctagcttc ctagtttgct
    12541 ctttgatttt cattgagtat aagattataa atgaaggagt atgacatgaa aaagtggtgg
    12601 aaagagctga tagacaagcc tttattaaaa gcttttttgc attattatca agcatcagat
    12661 agtgagttga ccagtgtcgc agtagcctac tattggttga tttcgatttt ccccctgctt
    12721 ttggtggtgg tcaatatcct cccctatttt cagattcctg tgggagaatt tctgtgcttt
    12781 atgaaagacg ttctgccccc aagcctctat gaaggtgtgg aaaaaatagc cagagaagtt
    12841 ttgacgcaac cttcaacagg tttattgagt ttttctgttt tatcggcgtt atggagtttt
    12901 tcaaaatcta tgaatttctt gcaaaaagct tttaacaagg cttatggcgt cgaaaagagt
    12961 cgtggactta tttcccatca gatgctaagt ctcctagtca gttttggctt gcagctcctc
    13021 tttgcgtttg ccttgttttt gatcttattt ggtcagatga ttttggcttt acttgctcat
    13081 tattggacaa aagatggcat tatctatcag gctttacaag ggttggcagg tcctctgatt
    13141 tacgccctcc tctttgctat cttggtcatg ctttattatt ttcttcccaa tctatctaac
    13201 aggaaaatta gctataccct accaggcagt gcctttgttc tcttagtgat tttaggttta
    13261 ctaactctgt tctctagtta tctcaattac tatgtccatc atttggtgga tgtccgaatt
    13321 ttaggttccg ttcttcttgt tgttatgatg ttttggttta tcttgattgc taaaattgtt
    13381 atcctaggcg cggttatcaa tgccagtatg cagagtttga aagatccggt ctttaaaaaa
    13441 gactaactat ttatccatta caaaagcgca tagtatcaaa actttataga accttgatac
    13501 tatgcgcttt tgtgctttga cagttttttg tatagttttt gtccaagatt tctaaatcat
    13561 ctgattaaat tcctattttc ccctatcatt gtccctgttt tttcgaaaat ggaaggagta
    13621 taataaatct atcaataaaa tgataggagg aactttatgg gtcatatttt cttttttcta
    13681 agtgtctttt tggcagggat tctatccttc ttttctcctt gtatcttacc tttgttaccg
    13741 gtctatacag gagtgttact agatgataag gatggtgctc aggcttctag cggcaaattt
    13801 tcaatctcag ttactagttt attacgaact ctggccttta tagcaggaat ttcctttata
    13861 tttattttgt tgggctatgg agctggtttt ttaggcgatt tgctttatgc ttcttggttc
    13921 caatatctta ctggggcaat tattatcctt cttggtttgc accaaatgga gattctacac
    13981 tttaaggggc tttataagga aaagaggcta caactgcaag gacaggggca aaatggtaag
    14041 ggctatagtc aggcattttt attgggcttg acctttagtt ttgcttggac gccttgcgtg
    14101 gggccggttc tggggtctgt tttggccttg gcggcttcag gtggttcagg agcttggcag
    14161 ggagctggtc tcatgttggt gtatacgctg ggcttggcgc taccattctt gcttctagct
    14221 ctgacctcta gttatgtttt gaaacatttc cgaaaacttc atccctatct cggaatcctc
    14281 aaaaaagtgg gtggttttct cattattgtg atgggactct tggttctgtt tggaaatgct
    14341 tcaattttaa gtcaattatt tgaataaaat ggaaaggaat atcaatatga aaaaatggca
    14401 aacatgtgtt cttggagcag gttcgctcct ttgtttgacg gcttgttcag gcaagtccgt
    14461 gactagtgaa caccaaacga aagatgaaat gaagacggag cagacagcta gtaaaacaag
    14521 cgcagctaaa gggaaagagg tggctgattt tgaattgatg ggagtagatg gcaagaccta
    14581 ccgtttatct gattacaagg gcaagaaagt ctatctcaaa ttctgggctt cttggtgttc
    14641 catctgtctg gctagtcttc cagatacgga tgagattgct aaagaagctg gtgatgacta
    14701 tgtggtcttg acagtagtgt caccaggaca taagggagag caatctgaag cggactttaa
    14761 gaattggtat aagggattgg attataaaaa tctcccagtc ctagttgacc catcaggcaa
    14821 acttttggaa acttatggtg tccgttctta cccaacccaa gcctttatag acaaagaagg
    14881 caagctggtc aaaacacatc caggattcat ggaaaaagat gcaattttgc aaactttgaa
    14941 ggaattagcc taggaggtgt cttatgaatg ataagttaaa aatcttcttg ttgctaggag
    15001 tattttttct agccataacc ggtttctatg ttctattgat acgaaatgca gggcagacag
    15061 atgcctcgca aattgaaaag gcggcagtta gccaaggagg aaaagcagtg aaaaaaacag
    15121 aaattagtaa agacgcagac ttgcacgaaa tttatctagc tggaggttgt ttctggggag
    15181 tggaggaata tttctcacgc gttcccgggg tgacggatgc cgtttcaggc tatgcaaatg
    15241 gtagaggaga aacaaccaag tacgaattga ttaaccaaac aggtcatgca gaaaccgtcc
    15301 atgtcaccta tgatgccaag caaatttctc tcaaggaaat cctgcttcac tatttccgca
    15361 ttatcaatcc aaccagcaaa aataaacaag gaaatgatgt ggggacccag taccgtactg
    15421 gtgtttatta cacagatgac aaggatttgg aagtgattaa ccaagtcttt gatgaggtgg
    15481 ctaagaaata cgatcaacct ctagcagttg aaaaggaaaa cttgaagaat tttgtggtgg
    15541 ctgaggatta ccatcaagac tatctcaaga aaaatccaaa tggctactgc catatcaatg
    15601 ttaatcaggc ggcctatcct gtcattgatg ccagcaaata tccaaaacca agtgatgagg
    15661 aattgaaaaa gaccctgtca cctgaggagt atgcagttac ccaggaaaat caaacagaac
    15721 gagctttctc aaaccgttac tgggataaat ttgaatccgg tatctatgtg gatatagcaa
    15781 ctggggaacc tctcttttca tcaaaagaca aatttgagtc tggttgtggc tggcctagtt
    15841 ttacccaacc catcagtcca gatgttgtca cctacaagga agataagtcc tacaatatga
    15901 cgcgtatgga agtgcggagc cgagtaggag attctcacct tgggcatgtc tttacggatg
    15961 gtccacagga caagggcggc ttacgttact gtatcaatag cctctctatc cgctttattc
    16021 ccaaagacca aatggaagaa aaaggctacg cttatttact agattatgtt gattaatact
    16081 cttcgaaaat ctcttcaaac cacgtcagcg tcgccttacc gtatgtaggg tt
//
LOCUS       DAVBCV010000046        15641 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBCV010000046 DAVBCV010000000
VERSION     DAVBCV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.046"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(48..797)
                     /locus_tag="VQ516_001516"
     CDS             complement(48..797)
                     /locus_tag="VQ516_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2511937.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            930..3266
                     /locus_tag="VQ516_001517"
     CDS             930..3266
                     /locus_tag="VQ516_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET2511938.1"
                     /translation="MDKNKIMGLTQREIKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3368..4045
                     /locus_tag="VQ516_001518"
     CDS             3368..4045
                     /locus_tag="VQ516_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2511939.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4032..4829
                     /locus_tag="VQ516_001519"
     CDS             4032..4829
                     /locus_tag="VQ516_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2511940.1"
                     /translation="MLASEVIHAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4846..5949
                     /locus_tag="VQ516_001520"
     CDS             4846..5949
                     /locus_tag="VQ516_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2511941.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            5956..6147
                     /locus_tag="VQ516_001521"
     CDS             5956..6147
                     /locus_tag="VQ516_001521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511942.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            6250..7269
                     /gene="galE"
                     /locus_tag="VQ516_001522"
     CDS             6250..7269
                     /gene="galE"
                     /locus_tag="VQ516_001522"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2511943.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAIEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7271..8224
                     /locus_tag="VQ516_001523"
     CDS             7271..8224
                     /locus_tag="VQ516_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2511944.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRKLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDDRLKKIEFI"
     gene            8376..9041
                     /locus_tag="VQ516_001524"
     CDS             8376..9041
                     /locus_tag="VQ516_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511945.1"
                     /translation="MLSKEDYTEEIGLIKKQNYVEAELYPIVADIIKPTLKDSLSKRY
                     VFGRQRRGLGQIYYGLSNFPDIVILDKTYENKSRKSIKIEEWKKLRGCVEVKNLNYSL
                     ITEEKIKSTISNSFEHITGEMEQLTGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKE
                     EIDNTIVEVVNKRIETEEAGNSFDWWKNIKDLSFNYTDICLSKDCRQEWNEFVKRVKE
                     IEW"
     gene            9080..9181
                     /locus_tag="VQ516_001525"
                     /pseudo
     CDS             9080..9181
                     /locus_tag="VQ516_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(9232..9438)
                     /locus_tag="VQ516_001526"
     CDS             complement(9232..9438)
                     /locus_tag="VQ516_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2511946.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            9490..9966
                     /locus_tag="VQ516_001527"
     CDS             9490..9966
                     /locus_tag="VQ516_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2511947.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9975..10646
                     /locus_tag="VQ516_001528"
     CDS             9975..10646
                     /locus_tag="VQ516_001528"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2511948.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDFETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            10777..11115
                     /locus_tag="VQ516_001529"
     CDS             10777..11115
                     /locus_tag="VQ516_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2511949.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            11118..11747
                     /locus_tag="VQ516_001530"
     CDS             11118..11747
                     /locus_tag="VQ516_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2511950.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11987..13138
                     /locus_tag="VQ516_001531"
     CDS             11987..13138
                     /locus_tag="VQ516_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2511951.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            13451..14200
                     /gene="truA"
                     /locus_tag="VQ516_001532"
     CDS             13451..14200
                     /gene="truA"
                     /locus_tag="VQ516_001532"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2511952.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            14190..14963
                     /locus_tag="VQ516_001533"
     CDS             14190..14963
                     /locus_tag="VQ516_001533"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2511953.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14941..15402
                     /locus_tag="VQ516_001534"
     CDS             14941..15402
                     /locus_tag="VQ516_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2511954.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         4473 a         2907 c         3613 g         4648 t
ORIGIN      
        1 tgatatataa atgaaaacct tagctgtttg gccaaggttc atttcttcta tgcaagttct
       61 tctcttttct tatctgggtt ccagatgaag cttgcgataa agctaaagat gatggttagg
      121 atagcgagaa taatagcaag gatgagggca gggatgcgga taaaccacca gagtggattt
      181 ggttttttat cattgtgcca ttgtttcgtt tcttcgtcca gacgttggaa ttctgtttga
      241 atacgattgg cgactgtttc aatgccttca tcattcattt tcttgatatc tgagatatcg
      301 attggatttc caaagttcat atcgacacgt tcacggctaa tcaagccctt caaagtcatg
      361 ggaccggtgt aggtaaccgg catgatacgg accttggcca ttttggcaat cagtgctgcg
      421 ccccccttga catcgtttga gtggcggcta ccacttggaa acatgatgag agagcggtca
      481 ctttttttga gaacgttgat aggatatttg atggctgagg cgctgggatt ttcacggtcg
      541 atgggaaagg cgccacacat acgaatccac caaccaaaga tacggttggt aaagagttct
      601 ttttttgcca taaagatgaa ctgttttggc ttggtcgcaa aggccatata aacaggatcc
      661 caccaggtac ggtgaggcgc aactaaaata taattttcat cttgattagg aattttatca
      721 gtattatgat agtgagcatt gccattgatg gaccataaga gcaatacaac taatccacgc
      781 aaataagtat aaaacatgcg atctccttcg attgttttct tgttattatt ataccttatc
      841 aaaggagggc tggcaaactt ttcccttgac tagatgcata tttgggatga aattagaatt
      901 ctgttaaaaa aaatgatata atagaattta tggataaaaa taagattatg ggattaaccc
      961 aaagagaaat caaggaaaga caggctgagg gtttggtcaa tgactttacc gcatcagcca
     1021 gtaccagcac ttggcaaatc gttaaacgaa atgtctttac cctttttaac gctttgaact
     1081 ttgccattgc tttggccctt gcctttgtgc aggcttggag caatctggtc ttctttgctg
     1141 ttatctgctt taacgctttt tctgggattg tgaccgagct acgagccaaa cacatggtgg
     1201 acaagctcaa tctcatgacc aaggaaaagg tcaaaaccat ccgtgatggt caggaagttg
     1261 ctcttaatcc tgaagaatta gtgctaggag atgtcattcg tttgtctgca ggagagcaga
     1321 ttcctagtga tgccttggtt ttggaaggct ttgcggaagt caatgaagcc atgttaacgg
     1381 gagaaagtga tttggtgcaa aaggaagttg acggcttact tttgtcagga agtttcctag
     1441 ccagtgggtc agttttatct caagttcacc atgtcggtgc agacaactat gctgccaaac
     1501 tcatgcttga ggctaagacc gttaaaccca tcaactcccg tatcatgaaa tcgctggaca
     1561 agttggctgg ttttactggg aagattatca ttccctttgg tctggctctc ttgctggaag
     1621 ccttgctttt aaaaggcctg cctctcaagt catccgttgt aaactcgtcg acagctcttt
     1681 tgggaatgtt gcctaaggga attgcccttt tgaccattac ttcgctcttg actgcagtga
     1741 ttaagttggg cttgaaaaag gtcttggtgc aggagatgta ctctgttgag accttggcgc
     1801 gcgtggatat gctctgtctg gacaagacgg gtaccatcac ccaaggaaag atgcaggtgg
     1861 aggctgttct tccactgacg gaaacttatg gtgaagaggc tgttgccagc atcctgacca
     1921 gctatatggc ccatagtgag gataaaaatc caacagctca agccattcgc cagagattcc
     1981 aaggtcaagt agtctatcct atgctctcta atcttccctt ctccagcgac cgcaagtggg
     2041 gagccatgga gttagaaggt ttgggaatag ttttcttagg ggcacctgag atgttgctgg
     2101 attctgaggt cccagaagcc agagaggcct tggagagagg atcacgtgtc ttggtcttag
     2161 ctctcagtca ggagaaatta gaccatcaca aaccacagaa accatctgat attcaggctc
     2221 tagccttgct ggaaatcttg gatcctattc gagagggagc ggcagagacg ctgaactatc
     2281 tccgttctca ggaagtgggg ctcaagatta tctctggtga caatccagtt acggtatcca
     2341 gcattgccca gaaggctggt tttgcggact atcacagcta tgtcgattgt tcaaaaatca
     2401 cggatgagga attgattgct ctggcggagg agacagcgat tttcggacgt gtttcccctc
     2461 atcaaaagaa actcattatc caaacgctga aaaaagcggg tcatacaaca gctatgactg
     2521 gggatggagt taatgatatc ctggctcttc gtgaggcgga ttgttctatc gtaatggcgg
     2581 agggagaccc ggcaacccgt cagattgcca acctggttct cttgaactca gactttaatg
     2641 atgttcctga gattcttttt gaaggtcgtc gtgtggtcaa taacattgcc cacatcgctc
     2701 cgattttctt gataaagacc atctattcct tcctgttagc agtcatctgt attgccagtg
     2761 ctttactagg tcggtcagag tggattttga ttttcccctt cattccgatc cagattacca
     2821 tgattgacca gtttgtggaa ggtttcccac cattcgttct gacttttgag cgaaatatca
     2881 aacctgttga gccaaatttc ctcagaaaat ccatgcttcg tgccctacca agtgccctca
     2941 tggtcgtgtt tagcgttctt tttgtgaaaa tctttggaac gagtcaaggt tggtctgagt
     3001 tagaaatctc aactctcctc tattatctct tggggtcaat tggtttctta tccgtattta
     3061 gagcctgcat gccattcact ctatggcgag tcctcttgat tgtctggtca gtaggaggct
     3121 tcctagccac agctctcttc ccaagaatcc aaaaactgct tgaaatttca accttgacag
     3181 aacaaacgtt gcctgtttat ggtgtcatga tgttggtctt tacagtgatt ttcatcctga
     3241 ctagtcgcta tcaagctaga aaataaagaa agactgcaat ctgtggattg cagttttttt
     3301 aggtacaaga ttgccagccg aaatgtggta taataagagg taatagagtt ttggaaagtg
     3361 agagaagatg atttcaaaga gattagaatt ggtagcttcc tttgtgtcac agggggctat
     3421 tttactagat gtgggaagtg accatgctta tctgcctatc gagttggttg agagaggcca
     3481 aatcaaaagc gctattgcag gtgaggtggt ggaaggtccc tatcagtctg cggttaaaaa
     3541 tgttgaggct cacggcctaa aggagaaaat ccaagtccgt ttagccaatg gcttggcagc
     3601 ttttgaagag actgaccaag tgtctgtcat taccattgct ggcatgggtg gtcgtttgat
     3661 tgctaggatt ttagaagaag gtttggggaa gttagctaat gtagagcgtt tgatcctcca
     3721 gcccaataat cgtgaagacg acttgcgtat ctggctacag gatcatggat tccagattgt
     3781 agcagaaagc atcttagaag aagctggaaa gttttatgag attttggtgg tggaagcagg
     3841 acaaatgaag ctatcagcca gtgatgttcg ctttggtccc ttcttgtcca aagaagtcag
     3901 tccagtattt gtccaaaaat ggcaaaaaga agctgagaag ctagagttcg ctctcggaca
     3961 aatcccagaa aaaaatctgg aagaacgtca agttctagta gataagattc aagctatcaa
     4021 ggaggtgctc catgttagca agtgaagtga ttcatgctta tgaagccttt tgccctcagg
     4081 aattttctat ggagggagac agccgtggtc tgcaaattgg cactttagac aagggtatcc
     4141 aaagggtcat ggttgccctc gatattcgtg aagagacagt ggcagaggcc attgaaaagg
     4201 gtgtggactt gattatcgtc aagcacgcgc ctatctttcg tcctatcaag gacttgcttg
     4261 ccagccgtcc gcaaaatcag atttacatcg acctgattaa gcatgacatc gcagtttatg
     4321 tcagccatac caatattgat atcgttgaaa atgggctcaa tgactggttt tgtcagatgc
     4381 taggaatcga ggagacgact tatctgcagg aaacaggtcc agaacgtgga attggacgta
     4441 ttgggaatat tcagcctcag acattttggg aattggccca acaggtcaag caagtctttg
     4501 acctagatag ccttcgaatg gtgcattatc aagagaatga tttgcagaag cctatttcaa
     4561 gagtagcaat ttgtggtgga agcgggcagt ctttctataa ggatgctttg gcaaaggggg
     4621 cagatgtcta tatcactggt gatatctact accatactgc tcaggatatg ctgtctgatg
     4681 gcttgttagc attggatcca ggtcactata tcgaagtgct ttttgtggaa aaaatcgcag
     4741 cactccttac tcaatggaaa gagaaaaaag gctgggaact agagatttta cctagtcaag
     4801 catcgaccaa tcctttccgt catatctagt tagaaagtga agacaatgaa aaaagttgcc
     4861 attattggag caggaattgt gggagcaaca gctgcctact acctctcgag agaaagtgac
     4921 ctagaggtga ccgtttttga ccatgagcaa ggtcaagcca ccaaggccgc agcaggaatt
     4981 atcagtcctt ggttttccaa acgccgtaat aaagcctggt acaagatggc gcgcttgggg
     5041 gctgattttt atgtggattt attagctgat ttagagaaat caggacaaaa aatcgacttt
     5101 taccagcgtt cgggagtctt tctcttgaaa aaggatgaat ccaagttgga agaactttat
     5161 caactggccc tccagcgcag agaagaatct cccttgatag ggcaattagc cattctggac
     5221 caagcctcag ctaatgaatt attccctggt ttgcagggat ttgaccgcct gctctatgct
     5281 tctggtggag cgagagtaga tggccaactc ttggtaactc gtctgctaga agccagtcat
     5341 gtcaagctgg tcaaagaaaa agtgactctg acaccgttag catcaggcta ccagattggt
     5401 gaagaggagt ttgagcaggt tattttggcg acgggagctt ggttggggga catgttagaa
     5461 cccttgggtt atgaagtaga tgttcgtcct caaaaaggac aactacgaga ttatcagctg
     5521 gtccaagaca tggaagctta ccctgttgtc atgccagaag gggagtggga tttgattcct
     5581 tttgcaggtg ggaaattgtc cttaggcgct acccacgaaa atgacatggg atttgatttg
     5641 acggtagatg aaaccttgct ccaacaaatg gaggaggcca ccttgactca ctatctgatt
     5701 ttggctgaag ctacttcaaa atctgagcgt gttggaatcc gtgcctacac cagtgatttc
     5761 tctcctttct ttgggcaggt gcctgactta actggtgtct atgcagccag tggactaggt
     5821 tcatcaggcc tcacaactgg tcctattatt ggttaccatt tagctcaact gatccaagac
     5881 aaggagttga ccttggaccc tgaaaactac ccaattgaaa actatgtcaa acgagtaaaa
     5941 agcgaataat actcaatgaa aatcaaagaa caaactagga aactagccgc gggttactca
     6001 aagcactgct ttgaggttgt ggatagaact gacgaagtca gctcaaaaca ctgttttgag
     6061 gttgtagata gaactgacga agtcagtaac catacccatg gcaaggcgac gttgacgtgg
     6121 tttgaattgg attttcgaag agtataagca ttttactgaa attttatcag gtagtttagg
     6181 atggcaaatg acattccctg tcaaaaatgg taaaataaga aaaaataatc tgagaatcga
     6241 ggaaaaaaga tgcaagaaaa gattttggta actggtggtg ccggttttat tggaacccac
     6301 actgttattg aattgatcca agcaggccat caagttgttg tggtggataa tcttgtcaat
     6361 agcaatcgta agagtttaga agttgttgaa agaatcacag gagttgagat tcctttctat
     6421 gaggcagata ttcgtgacac agctaccctc agagatattt tcaagcagga agaaccgact
     6481 ggtgtcattc actttgctgg tttgaaggct gttggcgaat caacacgtat ccctcttgcc
     6541 tactatgaca acaatatcgc tggaactgtc agccttttga aagccataga agaaaacaac
     6601 tgtaaaaaca tcatcttcag ttcttctgcg acagtttacg gggatccgca cacagtgccc
     6661 atcttggaag atttcccact ttcagtgacc aacccatacg gtcgtactaa gctcatgcta
     6721 gaggaaattt tgactgatat ttacaaagca gactcagaat ggaatgttgt cttgcttcgt
     6781 tactttaacc caatcggagc ccatgagagt ggtgatttgg gagaaaatcc aaacggtatt
     6841 ccaaacaatc tcttgccata tgtgactcaa gtagccgttg gaaaattaga gcaagtgcaa
     6901 gtgtttggag acgattacga tacggaagat ggaacaggtg ttcgtgacta tatccacgtt
     6961 gtcgatttgg ctaagggtca cgttgcagct ttgaaaaaaa tccaaaaagg ttcaggacta
     7021 aacgtttata accttggaac tggtaaaggt tactcagttc ttgaaattat ccaaaacatg
     7081 gaaaaagcgg tgggacgtcc tattccttac cgtatcgtag aacgtcgccc aggtgatatc
     7141 gctgcctgct actcagaccc agcaaaagct aaagcagaac tcggttggga agcagaactc
     7201 gacatcaccc aaatgtgtga agacgcatgg cgttggcaaa gcaagcatcc aaatggattt
     7261 gaagactaag atgatgattt caatcatcgt cccttgttta aacgaagagg aagtacttcc
     7321 tcttttttat caggctctgg aagctttact tccagatttg gaaacagaaa tcgagtatgt
     7381 ctttgtcgat gatggatcaa gtgatgggac cttggaactc ttaaaggcct atcgggagca
     7441 aaatccggca gtccattata tttctttctc tcgaaatttt ggcaaagaag cagcccttta
     7501 tgcaggcttg caatatgcga caggagattt ggtggtggtg atggatgcag acctccaaga
     7561 tcctcctagt atgttgtttg agatgaaaaa tgtactagac aaaaatgtag acttggactg
     7621 cgttgggaca cggagaacta gtcgggaggg agaacccttc tttcgcagtt tctgtgctgt
     7681 tctcttttat cgcctcatgc aaaaaatcag cccagtggct ctgccgtcgg gtgtccgtga
     7741 ttttcgtatg atgagaaggt ctgtagtcga tgccatttta agcttgaccg agtccaatcg
     7801 tttttcaaag gggctcttcg cctgggtggg tttcaaaacc tactatttgg actatccaaa
     7861 tgtcgaaagg caggctggca agaccagttg gagttttagg aaactctttt tctactccat
     7921 tgaagggatt attaattttt cagattttcc cttgattata gcctttgtag ctggtctcct
     7981 atcttgtttt ctttctctgc tgatgacctt ttttgttgtg gttcggaccc tcattttggg
     8041 caatccgaca tctggttgga cctctctgat ggctgttatt ctctttcttg gaggcattca
     8101 actcttgacc attgggattc tcggcaagta tatcagtaag atttatttag agactaaaaa
     8161 aagaccactt tatcttatca aagaaaaaag tgatgatagg ctaaaaaaga tagaatttat
     8221 ctgacatagt cactttttgt tatttgtaat tcttttagtt cggagtttgg gtgattttta
     8281 aaagaattag ggatttgaaa tgatttaaaa aatctatgta gactaaattt aaattgtagt
     8341 gacttaataa ataaaaaatt taggagtatt atgttatgct aagtaaagag gattatactg
     8401 aagaaatcgg gctaataaag aaacaaaatt atgtagaggc tgaattgtat cctatagttg
     8461 ctgatataat taagccaact ttaaaagata gtttatcaaa aagatatgtc ttcggaagac
     8521 aaagaagggg tttgggacaa atttattatg gtctaagtaa ttttcctgat atagttattt
     8581 tagataagac ttatgaaaac aaatctagga aaagtataaa aattgaagaa tggaagaaac
     8641 ttcgaggatg cgtggaagtt aagaatttaa attatagttt aattacagag gaaaaaatca
     8701 aatcaaccat atcgaacagt tttgaacata taactggaga gatggaacaa ctaactggag
     8761 agatgggaca acttattgga gaccttttat ggtataaaaa agtaatttat acaaatggaa
     8821 ttgaatggag atttctaagt ttagatgaca aggaagagat agataataca attgttgaag
     8881 tagttaacaa aagaatagaa acagaagaag cgggaaattc ttttgattgg tggaagaata
     8941 ttaaagattt atcgttcaat tatacggata tatgcctatc taaagattgt agacaagagt
     9001 ggaatgaatt tgtaaaaagg gtaaaggaaa tagaatggta gaagtctatt ctcattgaga
     9061 aatttagatt ttaagttaca gtaagattta tttagagact aaaaaaagac cactttatct
     9121 tatcaaagaa aaaagtgacc ttcctgattt tacagaaaaa aataaagtga aaagactata
     9181 attttacatg gaaatgtgct aaactagagg gagtggatta tcgccattga tttaggcttc
     9241 ctggtttcca atcagggcgc gagttgggca atttttaaca gcctctaaga catcctgact
     9301 aggagaaatt tctttttcca gttggtcagg gtcatcgtaa aaacgcacga ttccattatc
     9361 gtggtaatca aataaatcag aataagtttg gcaaagccca caggcgatac atcgttcagg
     9421 tataagtgtg attttcatgt tcatatttat attgtaataa gaaaagtaaa aaaaaacaag
     9481 gagtaaggta tggcaaaaga accgtggcaa gaagatatct atgatcaaga agaatcaaga
     9541 gcagagcgtc ggcatcgaaa ccacggaggg gctgatagga tggctaatcg tattttgacg
     9601 atcctagcta gtattttctt tgtaattgtg gtggtgatgg tcatcgttct catctatcta
     9661 tcatcggggg gaagtaatcg cacagcagcc ttaaaagact ttcatgattc tgatgcaagt
     9721 gtagtacaaa tctcatcttc aagcagttct cagcctgagc agagttcaga gccagaatct
     9781 acttctagta gttcagaaga agctgctaat cctgaaggaa cgattaaagt tctcgcagga
     9841 gaaggggaag cagctattgc cgctcgtgca ggaatctcca ttgctcagtt agaggccttg
     9901 aatcctgggc acatggctac aggatcttgg tttgctaatc caggtgatgt tataaaaata
     9961 aaataggagc cgatatgaaa acaattcaaa ttgctattga tggtcctgct tccagcggta
    10021 agagtacggt cgcaaagatt attgctaagg attttggatt cacctacctt gatacaggag
    10081 ctatgtatcg tgcagcgacc tatatggctc ttaagaacca attaggagtt gaagaagtcg
    10141 aagcccttct agccttgttg gaccagcatc caatcagctt tggacgttca gaaactggag
    10201 accagcttgt ttttgtagga gatgtggata ttacccatcc tatccgtgaa aatgaagtga
    10261 ccaatcatgt ttctgctatt gcagcaattc ctgaagtgcg tgagaaactg gtttctctcc
    10321 aacaagaaat tgcccagcaa ggcgggattg tcatggatgg tcgcgatatt ggaactgttg
    10381 tattgccaca agcagaattg aaaattttcc tagtagcttc tgttgatgag agagcagagc
    10441 gtcgttacaa ggaaaatatt gccaagggaa ttgaaacaga ctttgaaacc ctaaaaaagg
    10501 aaattgctgc gcgtgactac aaggatagtc atcgtgagac ttctcctctc aaacaagcag
    10561 aggatgctgt ctaccttgat acaactggtt tgaacattca agaagtagtt gaaaaaatca
    10621 aagcagaagc tgaaaaaaga atgtaaatgt agaaaagccg atagattgat atcggttttt
    10681 ttctaaattt gtcaaaatgc tgattttagg gtataatagt tgctgttcga gaaattttga
    10741 ttttcaaaga atagtgaatg atgataagga gaaaccatga acaatttacc aaattgtcca
    10801 aaatgtaact cagagtatgt ctacgaagac ggtgccctac tggtttgccc agagtgtgct
    10861 catgagtgga atcctgctga agttgcagaa gtagaagaag gtcttgtcgc tatcgatgcc
    10921 aacggaaata aattggctga tggtgataca gtaactctca tcaaggactt gaaagtaaaa
    10981 ggtgcgccaa aagatttgaa acaagggacg cgcgtgaaaa atatccgcat cgtagaaggc
    11041 gaccacaata tcgactgtaa gattgatggt tttggtgcca tgaaacttaa atcagagttt
    11101 gtgaggaaga tttaagcatg tcaaaacact ataaacttgt attttatagc cgtatcttct
    11161 tgtttctagc ggcttttacg ggagtttatc ttgaaattac taagcatggt ggttttggga
    11221 tgcttctcta ttacacggtt ctgtccaacc tcttggtaac tatttttacc ctttatcttc
    11281 taaaggttat gagccgtgta ggtgaaaatt ggcaaaggcc aagtctcttg cgcttaaaag
    11341 gtggggtcac catgagtatc atgattacct gtgtgattta ccatttcctc ttggcgccca
    11401 ttgcgactaa tttctatacc ctagaaaatt tcctttgcca ctatatcgtt cccatctggt
    11461 ttttagcgga taccctcttt tttgacaaac agggtcaata caagatttgg gatccagcag
    11521 tgtggaccat tttacccttt ctgtatatga tgtttgctct ttttaatggc ttggttctaa
    11581 aactcaatat tccaaatgcc aaggataatc ctttccctta cttctttttg aatgtgaaca
    11641 agggttggaa tgttgtgttt aagtggtgtc tgattatctt tgttgcctat atggtagcag
    11701 gatttatttt ctactttatc aagcaaatca agagaaagtc atcctaatac tcttcgaaaa
    11761 tctcttcaaa ccacgtcagt ttccatctgc aacctcaaaa cactgttttg aacaacctgc
    11821 ggctagcttc ctagtttgct ctttgatttt cattgagtat aagatacaag ggattcattt
    11881 tcgaatttaa gaaggagtct acaaaccaat gaggcttctt cttttcttgc ttatttgata
    11941 actatcaaaa aagtagaaga ttaagagaaa tatagaaaga gatttcatga aacaattttt
    12001 agaacgggcc agcattttgg ctctctccct cgttttgatt acctcctttt ccatttcgag
    12061 tgccctacca gccatgtttg actattatca gggttattct aaggaacaaa ttgagctctt
    12121 ggtgagcttg ccttcctttg gaatcatgat gatgttactg ctaaatggtt tcttagaaaa
    12181 aatatttcct gagcgcttac agattagttt gggcttgctg attttatcat tgagcggtac
    12241 agctcccttc tggtaccaag cctatccctt tgtctttgga acacggcttc tctttggttt
    12301 gggtcttggg atgatcaatg ccaaggccat ttctattatc agtgaacgct accaaggaaa
    12361 aaggcgaatt cagatgttag ggctacgcgc ttctgcagag gtcgttggag cttctctcat
    12421 taccttggcc gtcggtcagt tgttggcctt tggttggaca gctatctttc tagcctatag
    12481 tgctggattt ttggtgctgc ccctttatct gctctttgtc ccttatggaa aatcaaagaa
    12541 agaagtcaag aaaagagcga aggaagcaag tcgtttaact cgagaaatga aaggcttgat
    12601 ttttacctta gctatcgaag cggcagttgt agtttgtacc aatacagcta ttaccatccg
    12661 tattccaagt ttgatggtgg aaagaggatt gggggatgcc cagttatcta gttttgttct
    12721 tagtatcatg cagttgatcg ggattgtggc tggggtgagt ttttctttct tgatttctat
    12781 ctttaaagag aaactgctcc tctggtctgg tattaccttt ggcttggggc aaatcgtgat
    12841 tgccttgtct tcatccttgt gggtggtagt agcaggaagt gttctggctg gatttgccta
    12901 tagtgtagtc ttgacgacgg tctttcaact tgtctctgaa cgaattccag ctaaactcct
    12961 caatcaagca acttcatttg ctgtattagg ctgtagtttc ggagccttta cgaccccatt
    13021 tgttctaggt gcaattggct tactaactca caatgggatg ttggtcttta gtatcttagg
    13081 aggttggttg attgtaatct ctatctttgt catgtaccta cttcagaaga gagcttagga
    13141 ttgattccta agtttttctt ttgatactca atgaaaatca aagagcatag ttgattgagt
    13201 ttggaatagt acgctgtagc tgctaaaata tttctagaaa ttaatttgac ttttctaata
    13261 gagttattca tatcttattt caatctacta tagaaagcta gccgcaggtt gctcaaaata
    13321 gtgttttgag gttgcagatg gaaactgacg tggtttgaag agattttcga agagtatgag
    13381 atttttatac ccatacaata tttgttttag gtccttgttt acagactaat ttcttgataa
    13441 aatagaggtt atgacgagat ataaagcaac tatttcctat gatggttatg cctttgctgg
    13501 ctttcagcgc cagcctcatg cgcgtagcgt tcaggaagaa attgaaaaaa ccttgaccag
    13561 attaaataaa gggcaagcca ttactgttca cggtgctggt aggacagata gtggggttca
    13621 tgccctggga caggtcattc attttgacct gccttatcag atggatgagg agaaactccg
    13681 ttttgccttg gatacccagt ctcctgaaga tattgatgtg atttcgattg agcttgtggc
    13741 agatgatttt cattgccgtt atgccaagca tagcaagacc tatgagttta ctgtggatag
    13801 aggacgtccc aaaaatccga tgcgccgtca ctatgccacc cactttccct acccactcga
    13861 tgtggaacga atgcagattg caatcaaaaa gctagaggga acccatgatt ttaccggttt
    13921 tacagcctct gggactagtg tagaggataa ggttcgcacc atcacagaag ctagtttaat
    13981 agtcgatgag acaggacaat ttttgacctt taccttttca ggaaatggtt tcttgtataa
    14041 acagattcgc aatatggtgg ggactcttct caaaatcggt aacaaccgca tgccagtaga
    14101 gcagattgat ctcatcttgg agaagaagga caggcaactt gcaggtccca ctgcagcacc
    14161 aaatggtttg tatttaaagg agattcgtta tgaagaataa tcgtatttta gcactttctg
    14221 gaaatgatat ttttagtggt ggtggactgt cagctgattt ggctacctat accttgaacg
    14281 gcttgcatgg gtttgtagca gtgacttgtt tgacagcctt gacagaaaaa ggatttgaag
    14341 tctttccaac tgatgatacc atttttcaac atgaattaga tagcttgcgt gatgtggaat
    14401 ttgggggaat taagattggt cttctcccta ctgtcagtgt ggctgagaag gccttggact
    14461 ttatcaaaca acgcccagga gtacctgtgg tgttggatcc tgtcttggtc tgcaaggaaa
    14521 cgcatgatgt agctgtcagt gagctctgcc aagagttgat tcgctttttc ccttatgtca
    14581 gtgtgattac gcctaatctc ccagaagcag aattattatc cggtcaggaa attaaaacct
    14641 tggaagacat gaaaactgca gcgcagaaat tgcatgattt aggagcgcca gcagtcatta
    14701 tcaagggagg caatcgtctt agtcaggaca aggctgtgga tgtcttttat gatggacaga
    14761 cctttactat cctagaaaat ccagttatcc aaggccaaaa tgctggtgca ggttgtacct
    14821 ttgcctctag cattgccagt cacttgatta aaggtgataa acttttgcca gcagtagaaa
    14881 gctctaaggc tttcgtttat cgtgctattg cacaagcaga tcagtatgga gtaagacaat
    14941 atgaagcaaa caaaaacaac taaaatcgcc cttgtatccc tattaaccgc cctttctgtg
    15001 gttctaggtt atttcttaaa aatcccaaca cctacaggaa ttctaactct tttagatgct
    15061 ggtgtcttct ttgcggcctt ttactttggt agtcgtgaag gagcggtagt cggaggacta
    15121 gcaagtttct tgattgacct cttatcaggc taccctcagt ggatgttctt tagcttggtc
    15181 aaccatggct tgcagggatt tttcgcagga tttaaaggaa aaagtcagtg gttaggcctt
    15241 attttagcaa ctattgccat ggtaggaggc tacgccttgg gttctacttt gatgaatggc
    15301 tgggcagcag ccctcccaga aattctacca aatttcatgc aaaatatggt agggatgatt
    15361 gtaggattta ttcttagtca aagtatcaag aagattaagt aatactcttc gaaaatctct
    15421 tcaaaccacg tcagcttcca tctgcaacct caaaacactg ttttgagcag cctgaggcta
    15481 gctttctagt ttgcactttg attttcattg agtataaaga ggttgagtaa aaagtatttc
    15541 aaataagaaa aacgcatagt atcaggtgtt gaatgaactg ccccccaaaa agttagacag
    15601 aaaaaatcta actttttggg gtgtttttat tatgaaatta a
//
LOCUS       DAVBCV010000047        15606 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBCV010000047 DAVBCV010000000
VERSION     DAVBCV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.047"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(1..741)
                     /locus_tag="VQ516_001535"
                     /pseudo
     CDS             complement(1..741)
                     /locus_tag="VQ516_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567576.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ516_001536"
     CDS             complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ516_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2511955.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ516_001537"
     CDS             complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ516_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2511956.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3071..3466)
                     /locus_tag="VQ516_001538"
     CDS             complement(3071..3466)
                     /locus_tag="VQ516_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2511957.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3544..4305)
                     /locus_tag="VQ516_001539"
     CDS             complement(3544..4305)
                     /locus_tag="VQ516_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2511958.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4338..4964)
                     /locus_tag="VQ516_001540"
     CDS             complement(4338..4964)
                     /locus_tag="VQ516_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2511959.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4980..5297)
                     /locus_tag="VQ516_001541"
     CDS             complement(4980..5297)
                     /locus_tag="VQ516_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2511960.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5294..5593)
                     /locus_tag="VQ516_001542"
     CDS             complement(5294..5593)
                     /locus_tag="VQ516_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2511961.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5878..6069)
                     /locus_tag="VQ516_001543"
     CDS             complement(5878..6069)
                     /locus_tag="VQ516_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2511962.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6230..6904)
                     /locus_tag="VQ516_001544"
     CDS             complement(6230..6904)
                     /locus_tag="VQ516_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511963.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(6910..7356)
                     /locus_tag="VQ516_001545"
     CDS             complement(6910..7356)
                     /locus_tag="VQ516_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2511964.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7458..7973)
                     /locus_tag="VQ516_001546"
     CDS             complement(7458..7973)
                     /locus_tag="VQ516_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2511965.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVCVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLFLHVS"
     gene            complement(7970..8332)
                     /locus_tag="VQ516_001547"
     CDS             complement(7970..8332)
                     /locus_tag="VQ516_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HET2511966.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICSVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8398..10257)
                     /locus_tag="VQ516_001548"
                     /pseudo
     CDS             complement(8398..10257)
                     /locus_tag="VQ516_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10274..10744)
                     /locus_tag="VQ516_001549"
     CDS             complement(10274..10744)
                     /locus_tag="VQ516_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2511967.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(10868..11584)
                     /locus_tag="VQ516_001550"
     CDS             complement(10868..11584)
                     /locus_tag="VQ516_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2511968.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12465..13874)
                     /gene="ply"
                     /locus_tag="VQ516_001551"
     CDS             complement(12465..13874)
                     /gene="ply"
                     /locus_tag="VQ516_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2511969.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQHEKITAHSMEQ
                     LKVKFGSDFEKIGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLRQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFESLIKGVAPQTEW
                     KQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFL
                     RDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTP
                     KAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRT
                     ISIWGTTLYPQVEDKVEND"
     gene            complement(13885..14217)
                     /locus_tag="VQ516_001552"
     CDS             complement(13885..14217)
                     /locus_tag="VQ516_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511970.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14288..14896)
                     /locus_tag="VQ516_001553"
     CDS             complement(14288..14896)
                     /locus_tag="VQ516_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511971.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14909..15367)
                     /locus_tag="VQ516_001554"
     CDS             complement(14909..15367)
                     /locus_tag="VQ516_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2511972.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15492..>15606
                     /locus_tag="VQ516_001555"
     CDS             15492..>15606
                     /locus_tag="VQ516_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2511973.1"
                     /translation="MNYEASKQLTDTRFKRLVGVQRTTFEEMLAVLKTAYQK"
BASE COUNT         4505 a         3595 c         2859 g         4647 t
ORIGIN      
        1 ttgatatgag aatagtcttt ctcaacatcc ttatccaaca agcatgctgt ggcgtccttc
       61 ttaacttgaa ccaggtcaat attctgggct tgggctgcca tagacgcagc cacaataaca
      121 ttgacgatag atatcatact cctcacacat ctctactgaa cgtttgtagc cttgattttt
      181 cttgaaatcg ttgggaagat agtgggtcgt gtaaattttt tgcacatcga ttccgatgct
      241 attgatggtt tgagaattct tatgaggact gatggtcaag gctgaaccaa agtagtcaaa
      301 gcccaagtcc atagccactt gcgctgtttt atccagtcgg tagtcaaaac aaaccttgca
      361 acggtcgcca ccttcgggct cctcctctag tcccctaact agttttcggt attaattggg
      421 ttcgtaggga gcttctaggt actgaaccgt atttcctgtc cgatcattaa aatcactaac
      481 aaatttcttg atgacataga cccgcttatg gtattctact ttgggatgga tattagaatt
      541 ggcaaaatag atggtcacat ctgcatactt ggtcaaatat tctagtgtat aggtactaca
      601 aggggcacag caaacatgca cgagaatggt tggccgctgc tcatttttct cccatgcttg
      661 taccattttc tgcatgacac ggtcataatt aatcttctga ttggggggca tcttgcacag
      721 aatttcttct acatcgatca tgttcttctc ctttttctag tcttatttta tcatataagt
      781 tagaagagct caaatcaatt tagaagtgaa tattataaaa agaagggtta gtcattccct
      841 ccttttgtgt tttttataag ttgttttcta tagaaagctt acatcatccc gcccatcata
      901 cttggatcca ttgctggagc tggggctact ggttctggtt tattggctac gactgcttct
      961 gttgtcaaaa tcaagctggc tacagatgct gcattttgta gggctgaacg actcacttta
     1021 actggatcaa tgataccttg atcaatcatg ttaacccact cgccagttgc tgcgttgaat
     1081 cctataccaa gctcagcatt tttcaaacga tcgataacga tagatccttc aaatcctgca
     1141 ttgtgagcaa tttgacgaac gggttcttcc aaagcacgga gaacaatatt acgtcctgtt
     1201 gcttcatctc ctgtcaattc caaggtagca acagctggaa tcacattggc aagagctgtt
     1261 ccaccacctg caacaatacc ttcttcaaca gctgcacgag tagcgttgag ggcatcttca
     1321 atgcggagtt tcatttcttt caactcagtt tcagttgcgg ctccgacctt aataaccgct
     1381 acaccacctg acaatttggc caagcgttct tgcaattttt cacggtcaaa ttcagaagtt
     1441 gtagtttcga tttgagactt gataaccgca acacggtgag aaatcgcttc aggatttcct
     1501 gcaccttcta caataaccgt gctatctttg tccacggtca ctctcgctgc ttgaccaaga
     1561 gcttcaattg tcgcatcttt caactcaaga ccaaggtctt ctgtgataac tgttccgcct
     1621 gttaagatgg cgatatcttc aagcatggct ttgcgacggt caccaaaacc aggtgccttg
     1681 actgctacta cgttgaaggt tccacgaatc ttgttcaaaa caagagttgg aagagcctcg
     1741 ccatccacat catccgcaat aatcaagagt ggacgattgc tttggagaat gctttccaaa
     1801 agtggcaaga tttcttggat attggaaatt ttcttgtctg taatcaaaat gtacggattt
     1861 tcaaggtcag ccaccatttt ttcgctatca gtcaccatgt actgtgaaag gtaaccacgg
     1921 tcaaactgca ttccttctac gacttcaagc tctgtttcca taccacgtga ctcttcgatg
     1981 gtgatgacac cgtctttgcc aactttttcc attgcttcag agatgtactc accaactttt
     2041 tcagaacgag aagatacggc tgcaacttga gcgatagctt ctttattggc aacagggatg
     2101 gcgttgtttt tcaaagcttc aactgctgcg gcaactgctg tttcaatccc acgacgaata
     2161 ccgattggat ttgcacctgc tgtgacgttt ttgattcctt cacggacgat tgcttgggtc
     2221 aaaacagttg cagttgtagt tccatcacct gcgatatcat tggtttttga agccacttct
     2281 gataccaatt tggcacccat attttcaaaa tggtcttcta attcaatttc tttggcaata
     2341 gtcacaccgt cattggtaat caatggtgag ccaaatgatt tttcaagaac gacattgcga
     2401 ccttttggtc ccaaggttac tttaacagtg tctgcaagga tatcgacacc acggaccata
     2461 gctgaacggg catctgatga aaatttaatt tcttttgaca tacttacttt ctccttctat
     2521 tcttcaatga ttgccaagat gttagcttcg cctacgatga tgtacttttc atcgccatct
     2581 ttgacatcaa gacctgcgtg ggcttcaact aagacacggt ctccagtttt aacacttgga
     2641 gcaaccaagt caccgttcaa ggtacgaaca ccttgtccag tagccacaac ttgggctgtt
     2701 ttggtttttt cttgggctga acctgcgaga acaaagcccc caacggtttg ttctttttct
     2761 tctattttta agaccacacg gtcccctaat ggtttcaaca tctgatttcc tccataatga
     2821 gatagatatt attagcgctc tttatacctg agtgctaatt catagttcta ttgtatcact
     2881 tagtcagaaa taatcaagaa aaaagtctga ctttctcaag ataaaaagcc tgagaccaac
     2941 tcagactttt taatgtttat actcaatgaa aatcaaagag caaactagga agctagccgc
     3001 aagctgctca aagcattgct ttgatgttgt ggataaagct aacgtggttt gaagagattt
     3061 tcgaagagta ttaaaatggc aattcttcct cttccaagac taaatctgcc aaatcttggc
     3121 ctgcattatt ttcacgcatg gcacgttgag cacgactttc caagagttgg aatcctgtta
     3181 caagtacttc ggtcacgtag ttcatttggc catttttctc aaagcgacgg gtacgcaatt
     3241 ctccatcaac ggaaatgaga ctacctttgg ttgcgtagct tgccaaagtt tctgctagtc
     3301 tgccccatag gaccatattg acaaaatcag cttcacgttc accgttttgg tctttgtaac
     3361 gacggttcac agcgatagtt gctcgcgcta ccgacttgtc attgttggtt ttgtgcaatt
     3421 ctggtgtaga cgttaaacgc ccaatcatga taactttatt atacatattt tcttcctcct
     3481 acttatctat tcgtaggaaa tcaaaaaaag ttacagaaat ttgtaacttt tcgaggaaat
     3541 ttcttatttt ttatgaatca tgaaacctgt cgcctgttga ttggccataa tggtcatatc
     3601 tgtaatctga acacggcgag gctgactggt cacatagact actgtgtctg caatatcctg
     3661 agcttgcaag gcttctattc cttggtaaac ggacgcagct cgctctttat caccatgaaa
     3721 acgaacagtt gagaaatctg tttcgacaat cccaggctga atggttgtca ccttgatatc
     3781 cgttgcgatg gtatcaattc gcagtccatc cgaaaaggtc ttaaccgcag ccttggtagc
     3841 tgagtaaaca gcggcaccag cataggcgta gattcctgcg gttgacccca tattgataat
     3901 atgaccttga ttgactttta tcattgctgg caagaaacag cgagtaaccg ccatcagacc
     3961 tttaacattg gtatccaaca tggtcaacat atccaactcc tcatagtctt gatagggagc
     4021 caagtcaaga gccagtccgg cgttgttgac caagatatca atcctcccta tcgtttctag
     4081 aatatcagag caaacagtct tcaccatgac catatccgtc acatctagtg gaaaagtcca
     4141 gacggtttga tttggaaagc ttactgcaaa ctccgacttg aggatttcta atctgtctat
     4201 ccgtcgtcct gttagaacga catcctcacc ctgctccaga taagcacgcg caatcgcttc
     4261 accgattcct gaggttgctc ccgtaatcac tacatttttt gccatcttat ttccttctat
     4321 cagatgctga caagttccta ggcagtccag tgtttcgctg ggtcgaacgg tgttccgaca
     4381 acttggtctt ctgataattc aagcacccca cgtttttgcg gagcatttgg caaatgcaat
     4441 tcacgaggac tacacatcat cccaaaactc ttttcaccac gaagttcgcc tgggaaaatg
     4501 aaattccctt ttggcatcat ggcaccagga agagccacaa tggttttcaa cccaacacgc
     4561 gcattaggtg ctcctgcaac gatttgcact atcttgtcac ttgcgactgc aacttggcag
     4621 atgttgaggt ggtcactgtc tggatgagct accatctcga caatttcacc aacaacaaac
     4681 ttaggttcct tatcattaac aatttcttct gtaaaacctt ccgcctgtaa ttcttggttc
     4741 aaacgagcga cttgctcatc tgataaaaag acttgaccgc gctctgcaat ttcaaacaag
     4801 ctcgaaacct caaaaatgtt ccaagccact gtttccccat tttctttgag gaaaacacgg
     4861 gctaccttgc ctttgcgttc cacattcagt ttggcatctc cgctattttt cacgatgacc
     4921 ataaggacat cactgacatg ttctttgtta tacgtaaaaa tcattgtttc ctttttctcc
     4981 tatttcaatc ctgctaaaaa atcgttaatt tgctccttac ttttacggtc gcgattgaca
     5041 aaacgaccga tttccttgtc cttttctaga acaacaaggc taggaattcc gtaaacatcc
     5101 cagagtttgg ccaaatccat atactgatct cggtccattc gaataaaggt gaactctgga
     5161 ttggtctctt caatctctgg taaggcagga taaatataac gacaatcgcc acaccagtct
     5221 gccacaaaaa ggaagacctt cttgcccgct ttttccacta aagatgctaa ttcttctaaa
     5281 cttgctggct gtatcataag acttcctcct catagactag gtcttcattt tcatagacaa
     5341 aggtataatg acggccatcc tcaaaaatga cgccaccaac caagctctcc agactgcttt
     5401 cgtaaacttg aacataaagg gtcgcaattt cccccatgtc ggaaaaatgg tctcgcacaa
     5461 tctctgtcaa ctcttcctga gtcttcatga gcttacggtc atctgcaact tttttcgtag
     5521 caagagcaag gcttccgata cctagcagag ccaagcctgc catccacatt tttttagctt
     5581 tcataccatt cattttaaca caaaaaaggc ttcaggacaa atgaggaagc agcagaaaag
     5641 caagtaaaaa gcctcttcct ttaaggaaaa agacttctta tactaagagc aaactaggaa
     5701 gctagccgca ggctgctcaa aacacggttt tgaggttgta gataaggcga cgttgacgtg
     5761 gtttgaagag attttcaaag agtattattc ttattgccag gcacctaagt tgccaacgta
     5821 gtaactatca ggtgtgtagg tattgcgaag catcttacct gatgaagcca gataatacta
     5881 cttgccattg tctttgaccc aatcattcgc aatcatggaa ccagaagaac ttacataata
     5941 ccattctccc ttgtcataaa cccaagtact gactttcatg gttcctgagc aattaaaggc
     6001 aaaaaaactg tccaatatca ttcgtttttt aaaagcattt gacactacat catctacgct
     6061 gtagtacata gatacatcaa acgtaaaagc caaaatatcg aaaaatatac aacttttaaa
     6121 ggcagcattt cattttttaa agtctaacaa ccatagaact gaaatcagag caaggcatca
     6181 atttcctact cttagtacgt ctattttgat ggttgaagtg aagatttttt tatttaacgt
     6241 agacaatttc caacttccca aaagccacat ctccacggat agtcaaggtt ttgctggttg
     6301 gggctacagg agcgtcagcc ttagctgcac caaaggaggt ttctactttc aaatctacac
     6361 gccagtgttg tggaacatag acggttgcat tcccgaaggc cacttcaata tttaaagttg
     6421 caaaatcacc tagcatctct gcattatcat agtagatttt agcatcccca aaagcgactt
     6481 ccacttggtc atctacgaga tcttgatctt gcttatagaa ggtcccgcta ccaaaagcga
     6541 cttccttatc cgtgacgact gtttttttcc cattgtacca ccattttttt tcattccaga
     6601 acttacttga atgcgtcaga taaccaacac caagtaccac caagatgcta gcccaaataa
     6661 gagaatgatt ggtaactggt aacaagtcat aagcgtaatt tgcaatgatg agcgccagta
     6721 aacctgtaaa aactgccgaa gtgagatgac gtctaaggat ggactcaatg gacttataag
     6781 caaaaaaaac aatacctagt aaaggccata ttttaccatc caaagaagga attccaaaat
     6841 tcccttgcag caagatccaa gctgctaaaa ccaacaaaac aataccaaat gctttctttt
     6901 tcatgttttt tacctcatgt ttcttagatt ttcttttagg agggattggt aatgccgtga
     6961 gacatgaacc tccttgtgcg tctgataaaa ggaaatggtg cccgttcctg aaaaggactt
     7021 gtccactgag taaatctgac ggatgtttgc gatcgttgac ttggaaactc gattaaaata
     7081 gcgaggcaag atagactcca actcatagag cttgaggcga acctcataag cttccttctg
     7141 gttatgagcg tagatcttgc tcccttctgt ttcaaagaag agaatttctg acaggtctag
     7201 ataatattca cctgtcccct tgtaaaaagt caacctcgga gcctttgact cttgcaagag
     7261 ccgttgcaag tctgcaattt catctgtcaa agtgggggtc ttgatgacaa tttcagtctc
     7321 ctctaaattg ccgtcaatct caattcgtaa cttcatcaca tctcctttct gactctacta
     7381 tatcaaagct aagttaggaa tacaagggca aaaaagcaag tggtcaattt gaagcagtaa
     7441 gtggttgtag gactagctta acttacatgt aagaatagga agaaaaggaa tccccacacc
     7501 aaaccacaag catagccaac caagacatcc ttgggataat gaacaccccc taaaaccctc
     7561 accaacccca gcaaagctga aaagatcaag catactaagc caataggtag actagcatgc
     7621 agacaggcca tggagatgat agttgccgaa aagacatggc gactgggcat cgaccgtccc
     7681 gaactatcct tatcaagaag gggacagata tcccaagttt cataaggtct cgggtgattg
     7741 attttcttac gaaacaggga caaggccaca aaacctgatg caggaataag cacacaaacc
     7801 ccaacttgct gaccaagtcc tagttgcagg taagtggtga ctagcaagat cagatagacc
     7861 aggggcatgg cgactgtcat caaacgattg aaactgcgta ataggaataa aagaattgga
     7921 cgactagtta gtctggagct gatatttcga taccattctt gataattttt catttatgct
     7981 tctcatactt ttctagaact tgttgagatg ttttcccctt ggcaagccaa tccactagct
     8041 tgtccaagcg acgtatttcc tgcatcagag gatcatcaat ttcctcaata cggacactgc
     8101 aaatcttccc tgtaattgca attcgttcag ggacataata tggtgcttgt ctaaaaaaat
     8161 ctccgtaagt aacatcccta tccagacaag caagaacatc actcacttca taccctgtca
     8221 accaactggt cacttggtga actgactctg cctttccacc ctttcgctca acttttgcaa
     8281 tcaaggctaa gtagacagca gcaaatttca tattgtataa tttctgactc atacttctct
     8341 ctttctatgt gtactagtat aaataagaaa aagaaggccg tcaagccttc ttttgattta
     8401 ttcttctgct tcatcttctg taaattgact attgtacaag tcagcgtaga agccaccttg
     8461 cgccatcagt tcctcatagt tgccttgctc gatgatattt ccatctttca tgaccaagat
     8521 caagtctgca tttcggatgg ttgacaagcg gtgggcaatg acaaaggatg tgcgtccttc
     8581 catcaaacgg tccatggctt tttggatcaa ttcctctgtc cgtgtgtcaa cagaagaagt
     8641 cgcctcatcc aaaatcaaaa gcggtgcatc cttaagaagg gcacgagcaa tagtcaatag
     8701 ttgtttttgt cctacagaca aggtcacggt gtcatccaag atggtatcat agccatctgg
     8761 caaggtcata ataaagtggt gaattcccac agccttacta gcttccatca ttcgttcatc
     8821 actaatccct atttgattat agatgagatt gtctcgaata gttccttcaa agagccaggt
     8881 atcctgcaag accattgaaa aggcatcatg cacttctgaa cgcgtcatag ccttggtatc
     8941 cacaccatca atgtgaatac ttcccttatc aatctcatag aatttcatca aaagattgac
     9001 aatggttgtc ttaccagtcc cagtcggccc aacaatggca accttttgac cagcatgaac
     9061 tgtcgcagag aagtcatagt cttgaacatt gacaccgtcc accagaattt ctcctgctga
     9121 cacgtcgtag aaacgtggaa tcagattgac cagagttgat ttaccagaac ctgttgaccc
     9181 aataaaggcc actgtttgac cagtttctgc tttaaagcta acatgttcaa taactgcctc
     9241 cgaatttgcc gcatagcgga aggtcacatc cttaaactcg acctgacctt tgaagttttc
     9301 atcagtcagc tgcacttgaa cagggttttg gatagaagaa tgcaaatcta aaacttgatt
     9361 aatccgctta gcagagacca tagtttgggg aagaacgatg aagagtgctc ccatgagaag
     9421 gaagtccatg acaacctaca tggcataaga catgaaaaca atcatgtcac taaagagagg
     9481 cagacgcgct atcggagcag cgtcgttaat cacataggcc ccaatccagt aaatcgccac
     9541 actcaaacca cttgaaatcc ccatcatgat aggattcaaa atagccataa gacggttgac
     9601 aaacaaattt aaacgggtca attcatcatt tactgctgca aatttttcat tttgataatc
     9661 ctctgcattg taggcacgaa cgacacgaat acctgttaaa ctctcacgag tgatactgtt
     9721 cagtttatct gtcagcccct gaatcaagga ctgttttgga aaggctagcg tcatcaaaac
     9781 ggtcgtcatc aggacgttga caatcactgc cacaagtacg gcccagagcc agtattctga
     9841 atgacctaaa atcttcccaa tagcccagat agccataatt ggaccacacg ttaccacttg
     9901 caagtccata gtaatcaaca tttgaacttg agtaatgtca ttggtagtac gcatcaagag
     9961 gctaggaatt gaaaatttct taatctctgt ctgcgagtaa tccaaaactc ggttaaaaat
    10021 atcacttctc agcctactag tataagaagc cgccactcgg gatgcaaaaa atccaactgc
    10081 aactacggac aagaaggcaa gaaagggcat tcccatcatc atgcttgccg gctgccacaa
    10141 ctcatctaaa ttagttcctt gactacctag caaatccgta attttcgaga tataggtcgg
    10201 cacttccaac tctagataga ccgaaaagca agtaaagaga atggctagta aaatcatccc
    10261 ccattctttt ctactaattc ttttgactaa tttctttatt ctctcctcct attcccttga
    10321 tatttgcctg tagttgaccg agaactttct caaaaatcag taattcatct tcatcaatgt
    10381 cttccatcaa ctgcttgtct atgcgttcaa aaaaagcctt aacctgttgc atctgagaac
    10441 gtgctttgtc cgtcagacga acaaacttag cccgcttatc aacaggactc gcctccaatt
    10501 ccaccaaacc attttgcact atacgcttaa ccagattact agcaacagac ttggtaatat
    10561 tgagttcctg ctcgatatct ttaatcaaga ccaagtcttg gtttttctcg cgattatcca
    10621 aaaaatgcac aacctgacct tgcggcccac ccataaattc aatgccgcaa cgtttggctt
    10681 ccttttgcac catcaggtga atttgatgac caaaacgctt aaagactaac atcggtttat
    10741 ccataatctc ccccttctaa ataaaaatag ttctctggag aataattaaa tttctatgag
    10801 aactattttc ttgattaaaa aaatcccaag tgattttctc acttaggatc atgttctata
    10861 ggttaaatta aaacccatct acgttcgtat aaatcttttg gacgtcttcg tcgtcttcaa
    10921 gaacgctgta aagtttttca aaggtttcaa ggtcttcgcc tgacaattcc acttctgact
    10981 gaggaatcat ttccaattca gtcacttgga attcttcaat accagactca cggagggcaa
    11041 cgatagcctt gtgaaggtca gttggcgctg tgtaaactgt gattgtacct tcttgtgctt
    11101 ctacgtcatc cacatccaca tccgcttcga gcaattgctc aaagactgcg tccgcatctt
    11161 cacctccaaa tacaataaca cctttgttgt caaagaggta agaaacagaa cctgaagcgc
    11221 ccatgtttcc gccgttttta ccaaaggctg cacggacatt ggctgctgta cggttgacgt
    11281 tagaagtcaa agtatccaca attagcatag agccatttgg cccaaaacct tcgtaacgtc
    11341 cttctgtaaa ggtttcgtct gtgtttcctt tggctttatc aatcgcttta tcgataatgt
    11401 gttttggcac ttgggcttgt ttagcacggt cgataacgaa tttcaaagct gagtttgatt
    11461 ctggatctgg atcacctttt ttagctgcta catagatttc tacaccaaat tttgcatata
    11521 ctttagagtt ggctccatct ttagccgttt tcttggctac gatattggcc catttacgtc
    11581 ccattaggaa tctccttttt tcacatttta atctttctta ttataacaca agtttttttg
    11641 attttcacta gaggaaatgg attttattag caaatcaagc taggatagca ctttacctgc
    11701 taagatggtc ttgcctttct atctttatca acagacactc atccacattc aaaaaacaaa
    11761 ctagaccatt atctgcaaat agaaagtttc agccaagttt gacaaagtca gctcaaatta
    11821 ctgtttgaag tttgtagata taagcgacaa aaacaatcat actgcacctt ttgttgacag
    11881 tctactccag acatatcata gttcaagtaa atactttgaa atccaacagt tcttataggc
    11941 gctattgtat tctaagaaat caatagaaga gtttctaagc aaacctctaa tactcaataa
    12001 aaatcaaaga gcaaactaga aagctagccg caggttgctc aaaacactgt tttgaggttg
    12061 cggatagggc tgacatggtt tgaagagatt ttcgaagagt ataatttacg tgttcctaag
    12121 atggagaagt tagactagta cactggcact tctaaaccat tgctagcaat tgatttgttc
    12181 atatttaatt tcattttttc cataaatggg tattagatat aaacagcaaa atatttccga
    12241 tacgtgtcat tcttgaattt ccaatcatct aaaacaagta aaggataatc aatcccctgt
    12301 atatcaagga attggctacc ctttttactt ttttacacat cctgtttgat agactcattt
    12361 taacatcacg agcatactcc aatggaaatc gctaggcaag agataaactt tcagatattc
    12421 gcagagagct catcgcctct ttttgtcgca aacattcttc tctcctaatc attttctacc
    12481 ttatcctcta cctgaggata gagagttgtt ccccaaatag aaatcgtccg cttacgcact
    12541 agtggcaaat cggttttttc ataaaccgta cgccaccatt cccaggcaag cccggtacac
    12601 tctctaattt tgacagagag attgcgaaca ttccctttta aaggaatact agtggtaaag
    12661 tgagccgtca aatcctgccc atttctgtcc caagccttag gagtcaagac ttccttgcct
    12721 tgatgatcat aggataattc atcccaagta atataatatt gggcaacata ggcaccacta
    12781 tgatccagca gtaaatctcc gtttctgtaa gctgtaacct tagtctcaac atagtctgta
    12841 ctgttttgaa aggtcgcaac tacattgtca cgtaaaaaag aagttgtata ggaaatcggc
    12901 aagcctggat gatcggctgt aaagcgactg ccttcttgaa tcaagtcctc taccatatcc
    12961 accttgcctg ttacaactcg ggcacccgaa cttgggtcgc cccctaaaat aaccgccttc
    13021 acttctgtat tgtccaaaat ctgcttccac tctgtctgag gagctactcc ttttatcaaa
    13081 gattcaaaag cagcctctac ttcatcactc ttactcgtgg tttccaactt gagatagact
    13141 tggcgcccat aagcaacact cgaaatatag accaaaggac gctctgcaga aattcctctc
    13201 tgccttaaat cctctaccgt tacagtatct tgaaacacat ctcctggatt tttaacagca
    13261 tctacgctga ctgtataata aatctgctta aaattaacaa tctgaatctg cttttcgcct
    13321 gaatggacag agttaaaatc aatatcaaga gaattcccta tcttttcaaa gtcagaacca
    13381 aacttgacct tgagttgttc catgctgtga gccgtgattt tttcatgctg cattctagct
    13441 gggacattat tgacctgacc ataatcttga tgccacttag ccaacaaatc gtttaccgct
    13501 ccgcgaacac ttgaattgct ggggtcttcc acttggagaa agctatcgct acttgccaaa
    13561 ccaggcaaat caatactata agtcatcgga gcacgatcga ccgcaagaag agtgggatta
    13621 ttctctaaca aggtctcatc cactacgaga agtgctccag gatagaggcg actgtcgttg
    13681 gtagctgtta cagaaatatc acttgtattt gtcgacaagc tccgcttctt tctttcgata
    13741 acaacaaact catcgggtag ctgattaccc tctttgatga aacgattttc aatactttct
    13801 ccctgatggg tcaagagttt ctttttatcg taattcatag ctagtataaa gtcatttact
    13861 gctttatttg ccatcttcta cctcctaata agttcctgga ttgagttgca taaactcaga
    13921 cttgttcagc gaaatcagcc gtggttggac taagtaatcc aaaatttcct cgtacaattc
    13981 ttctgagaca ttgcgtcgcc gtctggctaa ataagaagtc ggaatgaccg tattatccaa
    14041 cataaatacc ttatctaagt caatcaaggt tggtcttgta aaaggattac gagctagatc
    14101 cggctcttct atcataaagt tcttgaccaa acgtctggtc aagagagctg gtttgaaggt
    14161 ctgattttta accaactctt tgtttttagt catgctgttg tcaatacaga tatacatatg
    14221 attcttcaca gccaaatcgc tactaatagt cggaaaaggc aaataaagag ctacaacatc
    14281 tcctctctta atcaagcaag agcaccccct tttctcctaa tgtaacatag acaggattga
    14341 ccaagtcttc tgattgactc agaatttcca aagtttgagt ttggcgcgct gtcaatttag
    14401 tagcatcttg tctcttcaat acaaaatgct tgtcgccaat aaccttgaca atataatcct
    14461 tctccaaagc tgactggtaa atccacatca gatgttgtct gtcctgagaa ctcaagagag
    14521 aaggattttc aagcctcccg atagtctgat aaaaatcaaa aacaggagct aactcctgcc
    14581 aatctgattg gctagttgtc aaggctagaa aaagggcttt gcgagctgat acttcttggt
    14641 tagccttgag agttactttc ccctccaagt tttttagaaa tcgggaaact ccagaaagca
    14701 aatttttctc taactgcgag aaataaaaac ctttcgttcc cagacataag tctttcatgt
    14761 cgctttctct agcaaataag agctcaaaca tttgatagta aaagaaaaat atctggcact
    14821 gggtcgcgct catcttttcc ttatcggctt ctttttttaa ccagagcaag ggcgacaggt
    14881 agctggattg agacatttcc tctacctcct actctttttt aactggagca tctgcactag
    14941 ctgccacttc ttttgactgg atactttccc actggttaat ctcctctgag ataagacctt
    15001 cgcatgtctt gacaaatagg gcaaaagcct tggtctttcc tgcatatttc tccgtttggc
    15061 attgatagag gaatttttct ttctccagga gttgcgcagt tttttggtaa gaaatccaat
    15121 tttcctttgc attatacaaa ttgataatcc cctcacacag caagccgaga ctggataagg
    15181 caaccgaaat caaacggtag cgatcacctg gcataggaat agcacaaaag acagctatga
    15241 ggaaacctgc cacgatttct gttattttta ataccttata gcgcctacga tgttgaacgc
    15301 ttttctttaa aaaatgagct atctgtacgt ctaatcgctc tgtcaggtac atttcttctg
    15361 gcgtcatatt cgtaactcct ttcatttact ttgataacca tagtataaca tatttgaata
    15421 tcaaagtata gtaaaaactg gactataact tattagactc cctgcgaaac aaaatatggt
    15481 atagtagttc tatgaattat gaagcaagta aacaattaac tgatacacga tttaagcgtc
    15541 ttgttggtgt tcagcgtacc acttttgaag agatgttagc tgtattaaaa acagcttatc
    15601 aaaaaa
//
LOCUS       DAVBCV010000048        15216 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBCV010000048 DAVBCV010000000
VERSION     DAVBCV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.048"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            205..1887
                     /locus_tag="VQ516_001556"
     CDS             205..1887
                     /locus_tag="VQ516_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511974.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            1872..2702
                     /locus_tag="VQ516_001557"
     CDS             1872..2702
                     /locus_tag="VQ516_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2511975.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLSLQNQGRFYNPWK
                     "
     gene            2895..3398
                     /locus_tag="VQ516_001558"
     CDS             2895..3398
                     /locus_tag="VQ516_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2511976.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      3452..3686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3851..4414
                     /locus_tag="VQ516_001559"
     CDS             3851..4414
                     /locus_tag="VQ516_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2511977.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTVAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYILLKPTLKHYER"
     gene            4428..5054
                     /locus_tag="VQ516_001560"
     CDS             4428..5054
                     /locus_tag="VQ516_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2511978.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            5166..6038
                     /locus_tag="VQ516_001561"
     CDS             5166..6038
                     /locus_tag="VQ516_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2511979.1"
                     /translation="MGMLLATRLKNKRKELKLSQKELAEGICEQGQISRMEQGKYSPG
                     SELLFQLSKRLKVSMDYFFEDTEVSSLENIDKFKELSKKFLDEREYESLHYIYELEKS
                     KRARLSAKDQIYLDWIDTLVQFCYLNHQTGAVAKLEKILLSLKKSDFYYLQLGNTLAN
                     FYFDMGQEDTYQELYQLLKENLQSLSVHHLEQLELMIKFRYNYCRYLWLNERTSEAIE
                     EITETIAICKRYNSSYRLADLYCLLGSISEGFAERDRILGFYQVSYQLYKHEENQRMM
                     LEIEKEMKLLESED"
     gene            6168..6305
                     /locus_tag="VQ516_001562"
     CDS             6168..6305
                     /locus_tag="VQ516_001562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511980.1"
                     /translation="MKRSQVVKRCILIALLLLFLLNVLSGSLSVTTRGVDPRPAYGVS
                     L"
     gene            6729..7643
                     /locus_tag="VQ516_001563"
     CDS             6729..7643
                     /locus_tag="VQ516_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET2511981.1"
                     /translation="MKLYQINDGIQIFESSNLEKIVIRNYRNGKKITIDHQPLLKKLL
                     LFLEVPKREEEILLYLPELSNKTLRSVLTNFMSLNIIHCQDTLIIHKKINILIIGLGT
                     TGGYLADGLCRSNINSKLFLLDKDRVDETNVGRQIYLRDDIGQYKVDVFKQKFSDKEI
                     KVFKIFINSKKDLEKICKREGIDLVIQCGDSPSPRELGKIVNTVCDTLQIPYIVNTGY
                     MSNVVPLPEFYYPNNRYNFNYKHQFSDEKLLFSQVLSKADYMVAIQSSFVMLMQIKNF
                     IFNEEPIYYGYRGYYNSVTLAWEVEKID"
     gene            7636..8649
                     /locus_tag="VQ516_001564"
     CDS             7636..8649
                     /locus_tag="VQ516_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511982.1"
                     /translation="MTKYLELDYSHSYILEGFRKNDAFIENHKECLDMLSDKIWRDNK
                     YMNTEKNISSPTRTILSRYTCNILSSLFIDISKNSIEKLIVTCENSDLNWIYNYYKSV
                     YNGVFCELRQLIQQYCKVKDKYEKWLFIKAIINQGIRQNEKEVVEFFLKQLKDNNQGI
                     FDYINSFSFYLLKFYSKDKSRIFLEEQLDIDDFLGSDKEDYARSLVFKNYASLLPDTS
                     ELKRNIMEKCLSQTPQDTDLWKEWFETYASQKEIRQKATEIFKHGYSDISLLKLVKIE
                     PDDTESLIRMIILCTSDLNSNIARYLISFLSDGRLKEFLELFVENFDFLNIIEGKTSE
                     INF"
     gene            8633..10225
                     /locus_tag="VQ516_001565"
     CDS             8633..10225
                     /locus_tag="VQ516_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2511983.1"
                     /translation="MRLIFRIIRKDIILYSILTFLLSIAMVSEAFLMQFIVDSIKFGE
                     KKYVLISIFIVIFLILQTLLYYYQQFLTAVLSKKSAYEYRKLIFKNIQQTSLRLVTGE
                     KNDSILASLTTQIDQIEYNYFYSVYWGGYLLCQLVVAIVMSLYLNPLMSVLTICLSLP
                     NLFVALLFKNSLEQKQEELINETNSSIAVTQDLIEGITDWKIANREPNIFRVFESKTL
                     KLLEKQVKVEKFQYIVVSLNQLFSNVLYFGSWIVGGFLIIRGQLSLGSLIAFSQLLAR
                     ISYPVYASSDLLSKYISGKKIVEILSKEFIFSDESRNYPDEFNVISFEHYFTNIDEKD
                     MPFDKEFYKNKKYLLKGKSGSGKTTILKSILREYDDYSGIVKINGVDVRDIKESVLFD
                     NIAYVSQKPHLFSASLRDNLTLFSEDFTDNEIVEVLKFVELSKWANKDCLDMMLSNDD
                     IKLSGGEVKRVSLARALLMNKNILLLDEFSAGIDYETLLKIEKKLIDLDKMIIYVTHV
                     DIDRSGKQFDDVIDLDCLFISS"
     gene            10417..11320
                     /locus_tag="VQ516_001566"
                     /pseudo
     CDS             10417..11320
                     /locus_tag="VQ516_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091364.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11338..11475
                     /locus_tag="VQ516_001567"
                     /pseudo
     CDS             11338..11475
                     /locus_tag="VQ516_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            11503..11844
                     /locus_tag="VQ516_001568"
     CDS             11503..11844
                     /locus_tag="VQ516_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511984.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFF"
     gene            11817..12128
                     /locus_tag="VQ516_001569"
                     /pseudo
     CDS             11817..12128
                     /locus_tag="VQ516_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            12256..12843
                     /locus_tag="VQ516_001570"
     CDS             12256..12843
                     /locus_tag="VQ516_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2511985.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            13233..14840
                     /locus_tag="VQ516_001571"
     CDS             13233..14840
                     /locus_tag="VQ516_001571"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2511986.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         4714 a         2370 c         3204 g         4928 t
ORIGIN      
        1 actgacgtgg tttgaagaga ttttcgaaga gtataaaatg atggggaagg ctgggcaact
       61 agtctttttc gatgtttata gattagttag gcaagtaagc taggatagga atctttttgg
      121 tgctaagatt ttaagagaag agactgcaag aagggcttga tttatgataa aatagaagtc
      181 taagaagcga atgaagagag taagatgaaa gaagctataa ttgagtggaa ggatttctct
      241 ttccggtatg aaacacaaca agaaccgacc ttgcaaggga tagacttgac catttacaag
      301 ggagagaaag tcttaattgt tggaccatct ggatcaggta aatctacctt gggtcagtgt
      361 ttgaatggga ttattcccaa tatttacaag ggtcagacat atggagaatt tttgataaag
      421 ggtcaaacag cctttgatat gagcatctat gataagtctc atctggttag cacagttttg
      481 caggatacag atgggcagtt tattggcttg tctgtggcag aagatttggc gtttgctctg
      541 gaaaatgatg tgacagccct agatgagatg aaaggtcgtg tttataaatg ggctgaaaag
      601 ctggaccttc ttcctttact ggatcagcgt cctcaggatt tgtcaggtgg acaaaagcag
      661 cgagtcagtc tggctggtgt cttgattgat gaaagtccga ttctcttgtt tgatgagcca
      721 ctcgccaatc tagatcccaa gtcaggtcag gatattatcg aattgattga ccagattcat
      781 aaggaagagg ggacgacgac tcttattatc gagcaccgtt tggaggatgt tctgcatcgc
      841 cctgtggatc ggattgtctt gataaacgat ggtcgtatcc tttttaatgg gagccctgac
      901 cagttgcttg cgactgattt attgactcaa aatggaattc gagaacccct ttatctaacc
      961 actctccgtc aattaggtgt ggacttagtc aaggaagaac agttagcgaa tctggataac
     1021 atgtctatct caaaaggtca ggttcagctg cagaatgaac tggtaaaaga aactccagaa
     1081 ttgcagtcac tctttaaatt agaggacgtg tctttctctt atgatgatag accgatttta
     1141 aaatccctac atttagatat taaaaagggt gaaaagattg ctattgtcgg aaaaaatgga
     1201 gcagggaaat caactctagc caaggctata agtagcttta ttcagacgga aggacgctat
     1261 ctttgggaaa aacaggatat aaaaggcgat tctgttgcag agcgggcgga acgagtaggc
     1321 tatgtgctac aaaatcctaa tcaaatgatt tcaaccaata tgatttttga tgaggtggct
     1381 ctagggctcc gtttgcgagg tgtggatgag aaggaaattg aaacgagagt ctatgaaacc
     1441 ttgaaaatct gtggacttta tgaattccgt aattggccta tttctgccct gtcatttggt
     1501 cagaaaaaac gtgtcaccat tgcttcaatt ttggtcttag gagctgaaat tattctccta
     1561 gatgaaccga ctgcaggtca agatcagaag aactatactg agattatgga atttctcgaa
     1621 gagttacatc gaaaagggca taccattgtc atgattaccc atgatatgca attgatgctg
     1681 gattattcag accgagtcct tgtcatggtg gatggagaat tgattgccga tactgttcca
     1741 gccagtctgt tgagcgatcc tgagctgtta gtaaaagcca atctaaaaga aacctccatc
     1801 tttaacttgg ctaagaaact agatgtggat ccactggatt taacggcatt ttacaaagaa
     1861 aggagagagg gatgcaagct aaattaatcg gttaccagca tagagatact gtgattcatc
     1921 gcttgtcagg agctgggaaa cttctctttt tcattctggt atcattggcg gccatgatta
     1981 gctatgatac cagaatgctc ttgctgattg ccatcttttc agtctttctc ctctatttat
     2041 cagaaattcg ctttaaagat gtttcctttg tagccgtttt tgcgacggta tttgccgttt
     2101 taaacgtttt gatggtctat ctcttttctc ccgagtatgg ggttggactt tatggagaga
     2161 gaagtgtgat ttggcaggga atcggtgtct acactctaac cagccaggag ctcttttatc
     2221 tgctaaattt ggccattaag tatctttgca ccattcctct ggctattatc tttttgatga
     2281 caacccatcc tagtcagttt gcttccagtt taaatcaaat tggtgtgccc tataagattg
     2341 cttattctgt cagcctgacc ttgcgctata ttccagattt gcaggaagaa ttctttacta
     2401 tcaagatgtc tcaggaggcg cgtgggatgg aattatccaa gaaagcttct cttatgcaac
     2461 gaatcaaagg caatctgctc attattacgc ccttgatttt tagctcgcta gaacgcattg
     2521 ataccattgc gactgccatg gagcttcgcc gctttgggaa agaaaaaaaa cgcacatggt
     2581 atagttatca ggccttgaaa aaaggagact atattacctt gctcttggca accttgtttt
     2641 tagtagctag tttactactt agcttgcaga atcagggacg attttacaac ccttggaaat
     2701 agcttgaaaa aattgaaaaa atcaagtcgt ttctattgac aatgattctg aaagtgttat
     2761 actaagaaag tagtttcgct gatttacttc aaacctgttg tgaggtaagt taacgatgcc
     2821 ttaaccacgc tgtttgctga gcttgactcc gggcagtgtg gctatttttt tgcaatggtg
     2881 aaaggaagca agtcatgaca aatcacattg tattatttga acctcaaatt ccacaaaata
     2941 cgggcaatat cgcgcgtact tgcgctgcga ccaattctcc cctccacatc atcaagccta
     3001 tgggctttcc tattgatgac cgcaagatga agcgggctgg attggattac tgggataagc
     3061 tagagattta tttttacgag agtttggaag atttcatatc tcagatgaag ggaaaactct
     3121 atctgatttc gaaattcgcg gaaaaagtct attctgaggt ggatttatcg actgacgagg
     3181 accattattt tctctttgga cgtgaagaca agggcttacc tgaagatttt atgcgagaac
     3241 atcctgagaa ggctctccgt attcccatga atgatgaaca tgtccgcagt ctcaatgtgt
     3301 ctaataccgt ctgcatgatt gtctatgagg ctcttcgcca gcagaacttt gcaggtcttg
     3361 aactcgtcca tacctatgaa gtggataaat tgaaataaaa aatgaaaatg aacaaaatgc
     3421 ttgcgcttgc aagcgttttt tgttatgata aaagagtctt cagggcaggg tgtgattccc
     3481 gaccggcggt gactttaact aggaaatgat cttttccttt tatactttgt tgacaagctt
     3541 tgcctaacca gaagttatgc ctacagcttg tcgcctagtc taaaagaaaa atctctattt
     3601 ccttctcttt aaagaagtcc gcgagcgcaa gctgatgtgg tgagattcca caaccgacag
     3661 tatagtctgg atgggagaag acgaaagaat agctttgtct gttttgatag atttatagcc
     3721 ggattgtaac cacttgcttt gtttttcttt aatagagtag gagggaactt ttgggatata
     3781 aaaagtgaga atagatagag gaatcctttc caacttcttc tgattttata gaaaattgga
     3841 ggaacctgtt atgacaaaca cacgtcgact ttcgaccatt gcaattttat cagccatctc
     3901 atttgtgctg atgtactttg actttccgct tttaccagcg gcatctttcc tcaagatcga
     3961 atttagtatc ttgccagtcc ttgtgggtct ggtggtcatg gatttgcctg ctgctctagg
     4021 agtgctcttg cttcgctcac tcttgaaatt gcttcttaac agccaaggag tgaatactta
     4081 cattggtttg ccgatgaata tcgtagcttt gggagttttt gtcatcgtat ttgctttgat
     4141 ttggaaaaag gaacggacaa cccttcgttt cctactaggc tctctagctg ggactattgg
     4201 tttaaccgtg gctatgttag ttctcaacta tgtttatgct gttcctttgt acgctaagtt
     4261 tgctaacttt gatattggaa aaattttggg actttccaac tacctaatga ccatggtatt
     4321 accttttaac ttgattgaag gtgtaatctt ttccgtttca ttctggttgt tgtacattct
     4381 cttgaaacca accttaaaac attatgaaag ataaacaaac atttttaatg aagggcagtt
     4441 ttgccctttt acttttcgtt attcttggct atatggtcaa attttaccct gaaatgttgg
     4501 tcaattttga ccaatcgatt cagactgcca ttcgaggaga cttaccagat tatttgacta
     4561 ttctttttcg atccctcaca cgtctgattg atattccagt gattatcact tgggttgtca
     4621 ttatagcttt tgtcttttat cgtaagcgat ggaagataga aagtttcttc atgctgggaa
     4681 atctgacttt ggcaggtctt ttaatcgtga cctttaaaaa tatctaccag cgcccacgac
     4741 cagctatctt acatctggtg gaggagaagg gattttcctt cccaagtggg cattctctgg
     4801 ctgtaacgct gatggtaggg actttgattg ttattcttag tcagcggatt aaagatcagg
     4861 tctggagaaa aatcgtacaa atcgtccttg gcctctacct agtcagtgtg ctggtatcaa
     4921 gagtctatct gggagttcat tacccatcag atgttcttgc cagtctctgt gtgggcttgg
     4981 gagccctgtt tatcgagttt cccttttatg ataagctccg cttccaatgg cgatttaaag
     5041 gcaaacagaa atgatggaaa agataaaaaa ttgtacaaat acaaatagaa ccatatgaaa
     5101 tatatacttg aaacatgttt tggagtttga tatattttaa taaataaagc aaggaggaag
     5161 aaagtgtggg tatgttatta gcgacacgct taaaaaataa aagaaaggaa ttaaaattat
     5221 ctcaaaaaga attagctgag gggatttgtg agcaagggca gattagccgt atggagcaag
     5281 gtaaatactc acctgggtca gaattactct ttcaactatc taagcgattg aaggtgagca
     5341 tggattattt ctttgaagat actgaggttt ctagtttaga aaatattgat aagtttaaag
     5401 aattatctaa gaaatttttg gatgagaggg aatatgaatc tctccattac atctatgaac
     5461 tggaaaaatc taaacgtgct cgtctatctg ctaaagacca gatttatttg gattggattg
     5521 atacattagt tcaattctgt tatttaaatc atcaaacagg ggcagttgcg aaactggaaa
     5581 aaatactgtt atctctgaaa aaatctgatt tttactatct acaactggga aataccttgg
     5641 ctaattttta ttttgatatg gggcaggaag acacatatca ggaactctat caacttttaa
     5701 aagagaacct ccagtcacta tctgttcatc atttggaaca gttagaattg atgattaaat
     5761 tccgatataa ctattgtaga tatttgtggt tgaatgaacg aacatctgaa gctatagaag
     5821 aaattacaga aacgatagct atttgtaaaa gatataatag tagttatcga ttagcagatt
     5881 tgtattgttt attgggaagt ataagcgagg gctttgctga aagggatagg atactaggtt
     5941 tttatcaagt atcttaccag ctttacaagc acgaagagaa ccagagaatg atgctagaaa
     6001 ttgagaagga aatgaaatta ttggaaagtg aagattaagc aaaaagattt gtattcttag
     6061 ataactttaa tacatattat aaaaaaaata aaaaaatatc ataaaaatta ttgacaaacc
     6121 aaaattgatg tgttagaata tcaccaatag ttaaggaggt ttagaagatg aaaagatctc
     6181 aagttgtcaa acgatgtata ttaattgctt tattgcttct atttctactt aatgtgttaa
     6241 gtggttcttt atctgtaaca actagaggag ttgatccaag accagcatat ggagtttcgc
     6301 tttaaagttg tgcatagttc ttcttttata gacggagagt ttttctcaat agtaagtgat
     6361 tcaaattttg atggatgatt ttccattaaa ttaagtgatt gcgaaaactt tttgaaatac
     6421 attctctttg gatattgaaa gtttgttttg gattttttta tgaagcttat cttagctgtt
     6481 agattggggt tttatgggat ttgtcctcac cgggaaaaaa tcttaaatct ttgaagaaag
     6541 aggtttttat cttatggtta gagatacatc agaagaaatt gaagttattg acttgtcttc
     6601 tatgaccgtt tcctactcat ctgttatgct aggaaactct tggatgacgt ggaattgatt
     6661 tattaagata gattatttgg tttttaccaa aaatctgtct ttttaatttt taaaggacta
     6721 atggtgatat gaaactatat caaataaatg atgggataca aatttttgaa agtagtaatc
     6781 ttgaaaaaat agtaattaga aattatagga atggtaagaa aataactatc gatcaccaac
     6841 cattattaaa aaaattacta ctattccttg aagttcctaa acgtgaagaa gaaattctat
     6901 tatatcttcc agaattatca aataaaacat tacgaagtgt tttgacgaat tttatgagtt
     6961 taaatataat tcattgtcaa gatacattaa taattcacaa aaaaataaac atattgataa
     7021 taggtttagg gactacgggt ggatatctgg cagatgggtt atgtcgtagt aatattaatt
     7081 cgaagctatt tcttttagat aaggatcgtg tagatgagac taatgtaggt agacagattt
     7141 atttaagaga tgatattggt caatataaag ttgatgtatt taaacaaaag tttagtgata
     7201 aagaaataaa ggtattcaaa atttttatta atagtaagaa agatttggaa aagatttgca
     7261 aaagggaggg tatcgatttg gttatacaat gtggtgactc cccttcacct cgagaattag
     7321 gaaaaattgt gaatacagta tgtgatactc tccagatacc ttatatagtt aacactggct
     7381 acatgagtaa tgtagttcct ttgccagaat tttattatcc aaataataga tacaatttta
     7441 attataagca tcaattttca gatgaaaagt tactgttctc acaagttttg tctaaagctg
     7501 attatatggt ggcaattcaa tcatcatttg taatgttaat gcaaataaaa aattttatat
     7561 ttaatgaaga acctatttat tatggttata ggggatacta taattctgta acattagcat
     7621 gggaggtgga aaaaattgac taaatattta gaattggatt attctcattc atatattttg
     7681 gaaggtttta gaaaaaatga tgcgtttatt gaaaatcaca aagaatgcct tgatatgtta
     7741 tcagataaga tttggcgaga taataaatat atgaatacag aaaaaaacat atcatcaccg
     7801 acacgaacaa tcttatcacg ctatacttgt aatattttat cgtctttgtt tattgatatt
     7861 agcaaaaata gcatagaaaa attgatagtg acatgtgaaa atagcgatct taattggatt
     7921 tataattatt ataaatcggt ttacaatgga gtattttgtg aattgcgaca acttatacaa
     7981 cagtactgta aggtgaagga taagtatgaa aagtggcttt ttataaaagc tattataaat
     8041 caaggtattc gtcaaaatga gaaagaagtt gttgaattct ttttaaaaca attgaaggat
     8101 aataatcaag gtatttttga ttacatcaat agttttagtt tttatctatt aaagttttat
     8161 tcaaaagaca aatctcgcat ttttttagaa gagcagctag atatagatga ttttttggga
     8221 tcagataaag aagattatgc taggtcttta gtttttaaga attatgcttc tctattgcct
     8281 gatacttctg aattaaaaag aaatattatg gaaaaatgtt taagtcaaac acctcaagat
     8341 acagatttat ggaaagaatg gtttgaaact tatgccagtc agaaagagat tagacaaaaa
     8401 gcaacggaaa tatttaagca tggttattca gatatttcgt tactaaaatt agttaaaatt
     8461 gagcctgatg acactgagag tttaatcaga atgattatct tatgtaccag tgacctaaat
     8521 agtaatattg caaggtattt gatttcattt ttgagcgatg ggcgattaaa agaatttttg
     8581 gagttatttg ttgaaaattt tgactttttg aatattattg agggaaaaac tagtgagatt
     8641 aatttttaga ataattagaa aagatattat attatattct atattaacat ttttgctatc
     8701 aatagcgatg gtaagcgaag catttttgat gcaatttatt gttgattcaa ttaaatttgg
     8761 tgaaaagaag tatgttttga tttctatttt tattgtaatt tttttgattc ttcagacttt
     8821 attgtattac tatcaacaat ttcttacagc tgtattaagt aaaaaatctg catatgagta
     8881 ccgtaaatta atttttaaaa atattcagca gacttctctt aggcttgtta caggtgaaaa
     8941 aaatgatagt attctagctt ctttgacaac gcaaatcgat caaatagaat ataattattt
     9001 ttattctgtt tattggggtg gatatttgct ttgccaatta gtggtagcta tagttatgtc
     9061 gctttatcta aatccattga tgtctgtatt aaccatttgc ttaagtctcc ctaatttatt
     9121 tgtagcactt ctttttaaaa actccttgga acaaaagcaa gaagagctaa ttaatgagac
     9181 aaattcctca atagcagtta ctcaagattt aattgaggga attactgatt ggaaaattgc
     9241 aaatagagaa cctaatatat ttagagtgtt tgaaagcaaa acattgaagc ttttagagaa
     9301 gcaagtgaag gtagaaaaat ttcaatacat tgttgttagt ttaaatcaat tgttttcaaa
     9361 tgttttatat tttggtagtt ggatagtagg tggattcttg attatacggg ggcaactttc
     9421 attagggagt ttgatagcgt tctctcaatt attagcaaga atttcttacc ctgtttacgc
     9481 ttcttcggat ttattgtcaa aatatattag cggtaaaaaa attgtagaaa ttctttctaa
     9541 agaatttata ttttcagacg aaagcagaaa ttatccagat gagtttaatg ttatttcatt
     9601 tgaacattat tttacaaaca ttgacgaaaa ggatatgcct tttgataaag aattttacaa
     9661 aaataaaaaa tatttgttga aagggaaaag tggtagtggg aaaacaacaa ttttaaaatc
     9721 gattttacga gagtatgatg attatagtgg aattgtaaaa attaatgggg tagatgttag
     9781 ggatataaaa gagagtgttc tttttgacaa tatagcttat gtttcccaaa aacctcactt
     9841 gttcagcgca agtcttagag acaacctgac attattttct gaagacttca ctgataatga
     9901 gatagttgag gttttaaaat ttgttgaatt atctaaatgg gctaataaag attgcttgga
     9961 tatgatgctt tcaaatgatg atataaaatt atcaggaggg gaggtcaaaa gggtttcttt
    10021 agctcgagcc ttattgatga ataagaatat tctgttattg gatgagttct ctgctgggat
    10081 tgattatgaa acattgttaa aaatagagaa gaaactcatt gatttggata agatgattat
    10141 ttacgtgacg catgtggata tagatagatc cggcaaacag tttgatgatg tcattgattt
    10201 ggattgtctt tttatatcct cgtgacatga gagttgagtc agttgaatta ttttgtcact
    10261 ctgtttagtt cttgttggta gtgtcaggta atctttgtta atttttcagg aagtctattg
    10321 aaatatcctc ttggttatat attacgagga gtaattaatg gatattctcg agaattcaat
    10381 acaacaattt gattctaaag ttaagtatgt ttctttattt gcggaatcaa tcttttatca
    10441 gataccatga gctattttgc aggtgctatg atttctcctt tgtatgtgag gattatcgga
    10501 gatgatatga cagatgctat gggggttaga caggcaactg ctggtgtagt taatattctc
    10561 agcaacctta tagggggagt tttgattggg ggtcatcagt cttcagatgt ttgtaggttt
    10621 gaatgctttg acctttcttt ttgcactgtt gggtgtggtg tttattcgta cttctttgag
    10681 agatattgag gaacagatgg aggttagtaa aagattagcc gcaaaatctt ttggagagca
    10741 tttcctccat tcaatgaaga tattgtcaag ttttccagcc atcatcaaat taatattcat
    10801 tgcagctatg aaccaagtga ttttaagcat tattatccct ttaacgactt tgatgttaat
    10861 ccatcatcca tttatcttct ttgacactgg gaaatccatt gcagcattgt cagtggttat
    10921 tttttcagga acgattgtgg gaagtttttt gagtggaggt atcctaaaaa atattcccat
    10981 aaaaatgatt gtctactttg aagaaattat gcatctctgt attctctttt cttggttgac
    11041 caatcatttc ctccttttat tggtagttgt cttcttatgc gcagttgcag taggaatgat
    11101 ggggcctcgg attttcaacc ttgtattctc gatggttcca gaagaagtca tgggaaccat
    11161 ccagtcagtg cttggagtat tcaatgttgt tgtaccagga attcttagca tgctcatcgt
    11221 tgccttagcg agtgctacaa acctaaaaat cacagtgctt ccacttttta tttttgtatt
    11281 gactgcctta gcaattgtga aagcaatgaa aatagagtaa tagtagtatg aactactgtc
    11341 tctttttggg tgggagttca ttacccatca gacgtccttg ccagtctctg tgtgggcttg
    11401 ggagttttgt ttatcgagtt tcccttctat gacaagctcc gcttccaatg gcgatttaaa
    11461 ggcaaacaga agtgagtatc gaattccctt gaggagaaag aaatgaaagt caatatagca
    11521 gatcttcatc cgactcaact atatttatca gaaaagaagt tgcaagatat tcagatgctt
    11581 taccagtcgg cagaaacaat ccaagtcgat ccaatcagta ttcttgcctt tggagattgt
    11641 ttgctgatta cagatgggca tcacagggct tatcaggctt tattggcagg tcgggatacg
    11701 atttctgctg agtgggatag agatggtggt gatgaactat atcatctcta tgcgcaagct
    11761 tgcgaggaaa gaaagattta ctctgttttt gatttaaaaa atcatatctt acctctttgg
    11821 atcggcatgg tgagcttttt ttgattgaag tcaaggagaa gggagaatgg tttccaattg
    11881 gggatattac actatctcag gataatctcc ccattgtgat tgggaattcc gcttaccaac
    11941 atcgaggact tggaaaaaag attctaagtg ctttgattga attggctcga gtaaaaggat
    12001 ggaaagaatt gagagtcaag gaaatctaca cctacaatca tgcttctagg aggtgtttca
    12061 agtcgcttgg atttgtggaa aatggagcaa cagaaaaagg aaggagtttt atattggaat
    12121 tagtctaatc ctgccacttt ctccccctta acctttgact attccgcaaa attatcgtaa
    12181 aataaagagt aaatgataaa atgaggtcag agtctgttcg ctctgacgat agtagtataa
    12241 atgaggagaa acgctttgga attagaagta tttgctgggc aagaaaaaag tgaactatct
    12301 atgattgagg tagcgcgtgc tatattagaa cttcgtggtc gcgatcatga gatgcatttt
    12361 agcgatcttg taaacgaaat tcaaaactac cttggaacat caaacagcga tatccgcgaa
    12421 gctttgcctc tgttctacac agagttgaac tttgacggta gcttcatctc acttggggac
    12481 aacaaatggg gtcttcgttc atggtatggt gtggacgaaa tcgacgaaga aatcatcgct
    12541 cttgaagaaa atgacgacga tgaagtagca ccaaaagcta agaaaaaacg tgtcaatgcc
    12601 tttatggatg gtgattcaga tgccattgac tacaatgcag atgatccaga agacgaagat
    12661 gcatacgaag cagatccagc tctttcatac gatgatgaaa atccagatga tgaaaaaaat
    12721 gaagtggaag cttatgatgc agaaatcaac gaaatcgctc cagatgactt gggagaagat
    12781 gtggatctca acgaagacga cgacgagttt tcagatgacg acgctgaaac cagcgaggaa
    12841 taagagttag ctattgacaa ttatcctatt tttaggtatc atattgtttg ggcacctctt
    12901 ttagaggtcg gggctcccta gttcttaggg agctattttt gttttttcaa gaagttatct
    12961 tcttgtattt tatactcaat gaaaatcaaa gagcaagcta ggaaactggc cgtaggctgc
    13021 tcaaaacact gttttgaggt tgtagataag actgacaaag tcaggaacac atatctacgg
    13081 caaggcgacg ttgacgcggt ttgaagagat tttcgaagag tattagttgt gaatctggtg
    13141 cagtcgtccc agattattct tattagtagg gtcttgtttt ctatatcccc tcgtagttaa
    13201 caagaccttg agcattttag aaagaggaat ctatgtctac gaaatatatt tttgtaactg
    13261 gtggtgtggt atcgtctatt gggaaaggga ttgtggcagc gagtctaggc cgtctcttga
    13321 aaaatcgtgg tctcaaagta accattcaaa agtttgaccc ttatatcaat attgatccgg
    13381 gaaccatgag tccttaccag cacggggaag tttttgtgac agatgacgga gctgagacag
    13441 atttggactt gggtcactat gaacgtttca tcgatatcaa tctcaacaaa tattccaacg
    13501 tgacaactgg gaaaatttac agtgaagttc ttcgtaaaga acgccgtgga gaataccttg
    13561 gggcaactgt tcaagtcatt cctcatatca cagatgcttt gaaagaaaaa atcaagcgtg
    13621 ccgctctaac gaccgactct gatgtcatta tcacagaggt tggtggaaca gtaggagata
    13681 tcgagtcctt gccattccta gaggctcttc gtcagatgaa ggcagatgtg ggtgcggata
    13741 atgtcatgta tatccataca accttgcttc cttacctcaa ggctgctggt gaaatgaaaa
    13801 ccaaaccaac ccaacactct gtcaaagaat tgcgtggctt gggaatccaa ccaaatatgt
    13861 tggttattcg tacagaagag ccagctggtc aaggaattaa aaataaactg gcccagttct
    13921 gtgatgtggc accagaagcc gttatcgaat cgttggatgt tgaacacctt taccaaattc
    13981 cactgaactt gcaggcacaa gggatggacc aaattgtttg tgatcatttg aaattagacg
    14041 caccagcagc ggatatgaca gaatggtcag ccatggtgga caaggtcatg aacctcaaga
    14101 aacaagttaa gatttccctt gttggtaagt atgtggagtt gcaagatgcc tatatctcag
    14161 tggtcgaagc cttgaaacac tctggctatg tcaatgatgt agaagttaaa atcaattggg
    14221 tcaatgccaa tgatgtgaca gcagagaatg tagcagaact cttgtctgat gcggacggga
    14281 tcatcgtacc aggtggtttt ggtcaacgtg gtacagaagg gaaaatccaa gccatccgct
    14341 atgcgcgtga aaatgatgtt ccaatgttgg gagtctgctt gggaatgcag ttgacatgta
    14401 tcgagtttgc tcgtcacgtt ttaggtcttg aaggtgccaa ttctgcagag cttgcaccag
    14461 aaacaaaata ccctatcatt gatatcatgc gtgatcagat tgatattgag gatatgggtg
    14521 gaacccttcg tttgggactt tatccgtcta agttgaaacg tggctctaag gctgctgctg
    14581 cttatcacaa tcaagaagtg gtgcaacgcc gtcaccgtca ccgttatgag tttaataatg
    14641 ccttccgtga gcagtttgag gcagcaggtt ttgtcttttc aggagtttct ccagacaatc
    14701 gtttggtaga aatcgtggaa attcctgaaa ataaattctt tgtagcttgt cagtatcacc
    14761 ctgaactgtc aagccgtcca aaccgaccag aagaactcta cactgccttt gttactgcag
    14821 cggttgagaa cagcaattag caaaatcaga acctttgaga aaaatctcag aggttttttg
    14881 catacgatga tattgcaata tatctgaggt agggggcctc tgtatgtacc tgctaccgtt
    14941 gaaatcaata gcgactccct cttgccctgt gctagtgaat ggatttatca gtatattgaa
    15001 atgaaataaa atttgaacaa attaattcgg aaagccaaat caatttctag caaagtttta
    15061 ggaactggat tgtatagtga attgaaataa gatgtgaaca tctctatcag aaaagtcaaa
    15121 ttaatttata gaaatatttt agcagtcaag atgtactgtt atagattcaa tacattatac
    15181 ttttttaatt taatccacta tagtaaaatg aaataa
//
LOCUS       DAVBCV010000049        14476 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBCV010000049 DAVBCV010000000
VERSION     DAVBCV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.049"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            3..350
                     /locus_tag="VQ516_001572"
     CDS             3..350
                     /locus_tag="VQ516_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2511987.1"
                     /translation="MLRLPYKKQKISHFTVSEDKDICRYIRQQLYYQNLFWMKEQAEA
                     YQKGENILTYGLKEWYPQIRPIVGKFFQIEQDLTSYYQHFYTYYQKNPQNDWQKLYPP
                     AFYQQYFLKNMVE"
     gene            369..2171
                     /gene="pepF"
                     /locus_tag="VQ516_001573"
     CDS             369..2171
                     /gene="pepF"
                     /locus_tag="VQ516_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2511988.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            2173..2886
                     /locus_tag="VQ516_001574"
     CDS             2173..2886
                     /locus_tag="VQ516_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2511989.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            2953..3894
                     /gene="prsA"
                     /locus_tag="VQ516_001575"
     CDS             2953..3894
                     /gene="prsA"
                     /locus_tag="VQ516_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2511990.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(4011..4352)
                     /locus_tag="VQ516_001576"
                     /pseudo
     CDS             complement(4011..4352)
                     /locus_tag="VQ516_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4765..4860
                     /locus_tag="VQ516_001577"
                     /pseudo
     CDS             4765..4860
                     /locus_tag="VQ516_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5113..5733)
                     /locus_tag="VQ516_001578"
     CDS             complement(5113..5733)
                     /locus_tag="VQ516_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2511991.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(5733..6215)
                     /locus_tag="VQ516_001579"
     CDS             complement(5733..6215)
                     /locus_tag="VQ516_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2511992.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(6215..7057)
                     /locus_tag="VQ516_001580"
     CDS             complement(6215..7057)
                     /locus_tag="VQ516_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2511993.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(7152..7952)
                     /locus_tag="VQ516_001581"
     CDS             complement(7152..7952)
                     /locus_tag="VQ516_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2511994.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            8096..9475
                     /gene="glmU"
                     /locus_tag="VQ516_001582"
     CDS             8096..9475
                     /gene="glmU"
                     /locus_tag="VQ516_001582"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2511995.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            9485..10030
                     /locus_tag="VQ516_001583"
     CDS             9485..10030
                     /locus_tag="VQ516_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2511996.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            10042..10356
                     /locus_tag="VQ516_001584"
     CDS             10042..10356
                     /locus_tag="VQ516_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2511997.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            10373..11065
                     /locus_tag="VQ516_001585"
     CDS             10373..11065
                     /locus_tag="VQ516_001585"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2511998.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            11142..11633
                     /gene="rocS"
                     /locus_tag="VQ516_001586"
     CDS             11142..11633
                     /gene="rocS"
                     /locus_tag="VQ516_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2511999.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            11731..12321
                     /locus_tag="VQ516_001587"
     CDS             11731..12321
                     /locus_tag="VQ516_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2512000.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            12330..14093
                     /locus_tag="VQ516_001588"
     CDS             12330..14093
                     /locus_tag="VQ516_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2512001.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            14307..>14476
                     /locus_tag="VQ516_001589"
     CDS             14307..>14476
                     /locus_tag="VQ516_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2512002.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASYVFQISRNTIYGWLKLKEK
                     TGELNHQVKGTKP"
BASE COUNT         4578 a         2587 c         3005 g         4306 t
ORIGIN      
        1 aaatattgcg tcttccctat aagaaacaaa aaatatctca ttttacagtt tctgaggaca
       61 aggacatctg tcgctatatc cggcaacaac tttattatca aaatctcttt tggatgaaag
      121 aacaagcaga agcctatcaa aagggagaaa atatcctgac ttatggactg aaagaatggt
      181 atccacaaat tcgaccaata gtgggcaaat ttttccagat tgaacaagac ttgactagct
      241 attatcagca cttttatacc tattaccaaa aaaatcctca aaatgattgg caaaagcttt
      301 atccaccagc cttttatcag caatatttct tgaaaaatat ggtagaatag aaaggatgga
      361 ggaatctaat ggtattacaa agaaatgaaa taaatgaaaa agatacatgg gatctatcaa
      421 cgatctaccc aactgaccag gcttgggaag aagtcttaaa agatttaaca gaacaattgg
      481 agacagtagc ccagtatgaa ggccatctct tggatagtgc ggataaccta ctagaaatca
      541 ctgaattttc tcttgaaatg gaacgccaga tggagaagct ttacgtttat gctcatatga
      601 agaatgacca ggatacacgt gaagctaagt atcaagagta ctatgccaag gccatgacat
      661 tctacagcca gttagaccaa gccttttcat tctatgagcc tgaatttatg gagattagcg
      721 aaaagcagta tgctgacttt ttagaagctc aaccaaagct gcaggtttat caacactatt
      781 ttgacaagct cttgcaaggc aaggatcacg ttctctcaca acgtgaagaa gaattattgg
      841 ctggagctgg agaaatcttt ggttcagcaa gtgaaacctt cgctatcttg gacaatgcgg
      901 atattgtgtt cccttatgtc ctagacgatg atggtaaaga agttcagcta tctcatggga
      961 tttacacacg tttgatggag tctaaaaaac gtgaggttcg ccgtggtgcc tatcaagctc
     1021 tttatgcgac ttacgaacaa ttccaacaca cctatgccaa aaccttgcaa accaatgtta
     1081 aggtgcaaaa ttaccgtgct aaagttcgta actacaagag tgctcgtcat gcagccctcg
     1141 cagcgaattt tgttccagaa agtgtttatg acaatttggt agcagcagtt cgcaagcatt
     1201 tgccactctt acatcgctat cttgagcttc gttcaaaaat cttggggatt tcagatctca
     1261 agatgtacga tgtctacaca ccgctttcat ctgttgaata cagttttacc taccaagaag
     1321 ccttgaaaaa agcagaagat gctttggcag tcttgggtga ggattacttg agccgtgtta
     1381 aacgtgcctt cagcgagcgt tggattgatg tttacgaaaa tcaaggcaag cgttcaggtg
     1441 cctactctgg tggttcttac gataccaatg cctttatgct tctcaactgg caggacaatc
     1501 tggacaatct ctttactctt gttcatgaaa caggtcacag tatgcattca agctatactc
     1561 gtgaaactca gccttatgtt tacggagatt actctatctt tttggctgag attgcctcaa
     1621 ctaccaatga aaatatcttg acggagaaat tattggaaga agtggaagac gacgcaacac
     1681 gctttgctat tctcaataac ttcctagatg gtttccgtgg aacagttttc cgccaaactc
     1741 aatttgctga gtttgaacac gccattcacc aagcagatca aaatggggag gtcttgacaa
     1801 gcgatttcct aaataaactc tacgcagact tgaaccaaga gtattatggt ttgagtaagg
     1861 aagacaatcc tgaaatccaa tacgagtggg ctcgcattcc acacttctac tataactact
     1921 atgtatatca atattcaact ggctttgcgg ccgcctcagc cttggctgaa aaaattgtcc
     1981 atggtagtca agaagaccgt gaccgctata tcgactacct caaggcaggt aagtcggact
     2041 atccacttaa tgtcatgaga aaagctggtg ttgatatgga gaaggaagac tacctcaacg
     2101 atgcctttgc agtctttgaa cgccgtttaa atgagtttga agcccttgtt gaaaaattag
     2161 gattggcata aaatggttga atcgtatagt aagaatgcta accataacat gcgtcgtcct
     2221 gtcgtcaaag aagaaattgt agacttgatg cgtcagcgtc aaaagcaggt cacaggtttc
     2281 ttgaaagaat tggaaaactt tgcccgcaag gaaaatattc ctattattcc ccatgaaacg
     2341 gttgcttatt tccgttttct tatggaaacc atgcagccta aaaatattct ggaaattggg
     2401 acggctatcg gtttttcagc tctcttgatg gctgaacatg cgccaaatgc taagattaca
     2461 actattgatc gtaatccaga aatgattggt tttgccaagg aaaattttgc ccagtttgac
     2521 agtcgcaagc aaatcactct cctagaggga gatgcggtgg atgtcttatc tacactgaca
     2581 gagtcttatg atttcgtctt tatggattct gccaagtcta aatacatcgt ctttctgcca
     2641 gaaatcctca aacatttgga agttggtggt gtggttgtct tggatgatat ttttcaaggt
     2701 ggtgatgttg ccaaggatat tatggaagtc cgtcgtggtc agcgaaccat ttatcgaggc
     2761 cttcaaaaat tatttgatgc aaccttagac aatccagaac tcaccgcaac attagtgcct
     2821 ttaggagatg gtattctcat gcttcgtaaa aatgtagcag atgttcaact gtctgaaagc
     2881 gaatgatttt cagaaaaatt taagaaaaaa tagtaaaata gatagagtaa cacttatctc
     2941 aaaggagtag acatgaagaa aaaattattg gcaggtgcca tcacactatt atcagtagca
     3001 actttagcag cttgttcgaa agggtcagaa ggggcagacc ttatcagcat gaaaggggat
     3061 gtcatcacag aacatcaatt ttatgagcaa gtgaaaaaca acccttcagc ccaacaagtc
     3121 ttgttaaata tgaccatcca aaaagttttt gaaaaacaat atggctcaga gcttgatgat
     3181 aaagaggttg atgatactat tgccgaagaa aaaaaacaat atggcgaaaa ctaccaacgt
     3241 gtcttgtcac aagcaggtat gactcttgaa acacgtaaag ctcaaattcg tacaagtaaa
     3301 ttagttgagt tggcagttaa gaaggtagca gaagctgaat tgacagatga agcctataag
     3361 aaagcctttg atgagtacac tccagatgta acggctcaaa tcatccgtct taataatgaa
     3421 gataaggcca aagaagttct cgaaaaagcc aaggcagaag gtgctgattt tgctcaatta
     3481 gccaaagata attcaactga tgaaaaaaca aaagaaaatg gtggagaaat tacctttgat
     3541 tctgcttcaa cagaagtacc tgagcaagtc aaaaaagccg ctttcgcttt agatgtggat
     3601 ggtgtttctg atgtgattac agcaactggc acacaagcct acagtagcca atattacatt
     3661 gtaaaactca ctaagaaaac agaaaaatca tctaatattg atgactacaa agaaaaatta
     3721 aaaactgtta tcttgactca aaaacaaaat gattcaacat ttgttcaaag cattatcgga
     3781 aaagaattgc aagcagccaa tatcaaggtt aaggaccaag ccttccaaaa tatctttacc
     3841 caatatatcg gtggtggaga ttcaagctca agcagtagta catcaaacga atagtccaaa
     3901 tcaatgagtc agggaaaaaa actcgacttc aggaaaaaat gaagcaaaca ttcccacaat
     3961 aaaacgcata gtacaaggtt tgtactgacc ccaaaagtta gacaattaat ttatctgaag
     4021 gatttagttc tgtattgcac agggctaagt ccttttagtt ttaccttaat tctcttattg
     4081 ttgtaataat caatatagtc tataatggct tgttccaatt gattaagtga tttaaatgtt
     4141 ttctcatagc cataaaacat ttcggatttt aaaatgccaa agaaagattc catcctaccg
     4201 ttgtcttggc tgtttccttt gcgtgacata gatgcttgaa ttcccttact ctctaggaag
     4261 cgatgataag aatcgtgttg gtattgccag ccttggtcac tatggagaat cgtattctcg
     4321 tagtgcttct ctttgaatgc ctgttccaac attaacgatc aatcaattta atcatgtacc
     4381 taatattaga attgtttatc ccaaatttat ttgaaagctt ctctaagcta tatccttgtt
     4441 ttctaagttc atagatctga actttatcat cataagttaa tttcataata aaaacacccc
     4501 aaaagttaga ttttttctgt ctaacttttg gggtgtagtt catgtacacc tgatatgatg
     4561 cgttttataa ttttaaagac tttttgacca gcctcatttt tttaacttga tactcagtga
     4621 aaagcaaaga ttaaactagg aagctagctg taggctgctc aaagaacagc tttgaggttg
     4681 cagataaaac ttgtgaggtc accaacatat ataatgtgaa gctgacgtga cttgaataga
     4741 ttttagaagg gtatgagtct ggaagtttta atggataatg caagattcca tagaatgggt
     4801 aagctagagt tcttatgtga agagtttggg cataaacttt taccttttcc tccctactca
     4861 tcttagtata gaaaagtgaa tctaaaatag tacataactg cttctaaaac attcttataa
     4921 attgatttaa attctcaaat catattattc agttcttatt tcattttgct ctacaatcct
     4981 gttgagaaga cacgtgttca tatcaaaaag gtattggcaa gttgcaatac ctttttacaa
     5041 ggttcttttg tcttattttt gtttcaactg actatatctc ctatggttct agttcagaag
     5101 gctaggctat aattatgatt gataagaagt atcattccaa gtattgagag tgaatgtttc
     5161 aaaatcatgg gtttctataa tggtcaggct ggcatttgct agaccgccat ctttacgaag
     5221 aagtggttct ttatagccta ggagagtacg aagactggca gtaagattgg cgccgtgtcc
     5281 gacaattaga atacgctcag ctggactatc ttttaatgat ttgataaatt ggatggtccg
     5341 ttgagttgta ctatagaggg attcggctcc gaacattcga gtgtcaaatt gagcaagatt
     5401 tgaacgaaaa gcctggattt gttgcgggta aatagcttcc aaggttgcaa ttttcaaacc
     5461 ttctaacttc ccaagttgcc attcacggag attaggaacg atttctaaag aacagggggt
     5521 atagagttga ctttggataa tctcagcaga tttgaccgct cgaggtaaat cacttgaata
     5581 aatctgatca aaaggaattt ccttgagata ctggccaagt cgttttaggg tttcaatgga
     5641 ttcaggaaga agaggagaat caccgctagc accttgaaaa cgaccttctt ggttccagag
     5701 ggtacgaccg tggcggacaa agtagagttt cattacttga tgtcctccaa aatatctaca
     5761 aagtctgcct ttacaaagct agccaagtct tgtggcgcga cgataatgct gtgtccgact
     5821 tcgcctgcag agacaatcat tcgatccaaa tctagagcaa ttttatcgat aaaaatggga
     5881 taattgtgtt tctgacgaat tccgacagga ttattggctc catgaatgta accagttgtt
     5941 ttttctaagt ccttttgtgg aatcatgctc acttttttat tgccagaaat tttagctagt
     6001 ttcttttcag acaagtgctg agtgataggg acaattccga taatcggtcc ggtcttgtct
     6061 cccaaaagcg ccaaggtttt gaaaatctga tctcgttcat aaccttgagg aagctctcct
     6121 tctagggcat tgatttgaat cccctgatga gggatagctg ctttagatag gatttgttcc
     6181 accaatgttt ttttgatttt aacttttttt gccattattt atatttatcc tccaattgac
     6241 tcatccaaat accaagccag attcccagcg caaagaagaa ggcgatgatg acataaccga
     6301 caagtgaaag tcctgtgtat tggatacttt cagcgtttcc tgcatttgga attaagatca
     6361 aaagggtact tgataggacg ataccgatga tgaaatgata gacgcgtgag tggtagttgt
     6421 ttaaggcata atccatcaat tttgaaaaaa cgatgagagt tgcacctgca ccaattccaa
     6481 tcggaaagaa ggttcccaag agatcaaaag ttttaaaacc agtcaacata ggagcataga
     6541 gtcccaaaat caaaagtaag ttcgatgggc tgaggccagg aaccaagacg ccaagggcca
     6601 atagtgcgcc tgctaggacg aagttaagaa agctggcgct taaggttcca acgacaaaat
     6661 ttaaggcata gagtcctaat ccagaaatga taaaggttgt ccataaccaa gctaaatcaa
     6721 tcttgtctcg gtcagattct cgagttgatt ctttgaggag gctaggaact gtaccgataa
     6781 tagctcccgc aaagctccat aatacaaaga cctgataatt ttcaagcagg tattcaatcg
     6841 ggtaggaaaa taagccgatt cccagaagca taccgatggc aactggaata aagtacaaaa
     6901 cattttcttt aaggtcttta aagggatggg ccagaaagcc aatcattcgt tcatagattc
     6961 ctaagattgc tgctagaacc cctccggaaa ttcccggtag gataaatcca agagcaatta
     7021 caatcccttt aataacgcgt gctaaccatg agagcatata tttcctccaa ctatttctat
     7081 ttcaaaaatt ccttactata ttgtatcaca atcagacaca aaaagaaaaa gcaaatgata
     7141 aacaaatgct tttaaggttt taaaaaaagt tttcgaaagg tttcttcttt attttttaag
     7201 ggagagataa cgttgatatc taaatcgtgg tcaaagccgg caatttttcc tttagatgtg
     7261 tattggtgaa tatcataatc taaatcagtt ttaggactgc tctccaaaaa tcctgagtct
     7321 gagccgtagg acggaatcca aacagaggta aacttgcctg tatcaatact gtgttcttcc
     7381 atgaagtaga caccaacgta gatgccgatg tttttagcac ctagtgatgc tagttttgct
     7441 cgaaagtttt cgacaccttc gttcatatta gacatggttt tgtcttccac gtcaagccaa
     7501 tagtaactag ggctgtaagg agaggcagca ttgtagaaaa cttcggcagc cttttccatt
     7561 tcttggacac tttttccagc tacataagcg tagacagcaa ctgggacatt ccgcttttga
     7621 agttcagtga tatgactctt ataggccttg tctattccat tgataaatga agcatcattt
     7681 tcttttgttg tttgagcacc actgtgaaca cgaacaatag cacctgaaat attttgtgag
     7741 agggcatcgt agttgatttc ctcaggacgc tgccagccag agaggtcaat aatcggtttg
     7801 tctaagtgtt tcaaagcctg tgcttcaatc tgtgctatat tggattttgt tttaaacgat
     7861 tggctgtcat taagtgggcg attgatgatt aaaatgaaca tcataatccc aaaaaaacta
     7921 aataaaataa gtggatgaat ttgttttctc atatcttata agtctaccct aaaaatcaaa
     7981 aaaaatcaaa aaaatgggtt aaggaagaga ctttagagca ttttttcatt caagagtgcg
     8041 gaatgatttg aaatatggta taataaaagg gaatttctac agaaaagaga agattatgtc
     8101 aaattttgcc attattttag cagcgggtaa agggactcgc atgaaatctg atttgccaaa
     8161 agttttgcac aaggttgcgg gtatttctat gttggaacat gttttccgta gtgtgggagc
     8221 tatccaacct gaaaagacag taacagttgt aggacacaag gcagaattgg ttgaggaggt
     8281 cttggctgga cagacagaat ttgtgactca atctgaacag ttgggaactg gtcatgcagt
     8341 tatgatgaca gaacctatct tagaaggttt gtcaggacac accttggtca ttgcaggaga
     8401 tactccttta atcactggtg aaagcttgaa aaacttgatt gatttccata tcaatcataa
     8461 aaatgtggcc actatcttga ctgctgaaac ggataatcct tttggctatg gacgaattgt
     8521 tcgtaatgac aatgctgagg ttcttcgtat tgttgagcag aaggatgcta cagattttga
     8581 aaagcaaatc aaggaaatca acactggaac atacgtcttt gacaacgagc gtttgtttga
     8641 ggctttgaaa aatatcaata ccaataacgc tcaaggcgaa tactatatta cagacgtcat
     8701 tggtattttc cgtgaaactg gtgaaaaagt tggcgcttat actttgaaag attttgatga
     8761 aagtcttggg gtaaatgacc gtgtggcgct tgcgacagct gagtcagtta tgcgtcgtcg
     8821 catcaatcat aaacacatgg tcaacggtgt tagctttgtc aatccagaag caacttatat
     8881 cgatattgat gttgagattg ctccggaagt tcaaatcgaa gccaatgtta ccttgaaagg
     8941 gcaaacgaaa attggtgctg agactgtttt gacaaacggt acttatgtag tggacagcac
     9001 tatcggagca ggagcggtca ttaccaattc tatgattgag gaaagtagtg ttgcagacgg
     9061 tgtgacagtc ggtccttatg ctcacattcg tccaaattca agtctgggtg cccaagttca
     9121 tattggtaac tttgttgagg tgaaaggatc ttcaatcggt gagaatacca aggctggtca
     9181 tttgacttat atcggaaact gtgaagtggg aagcaacgtt aatttcggtg ctggaactat
     9241 tacagtcaac tatgacggca aaaacaaata caagacagtc attggagaca atgtctttgt
     9301 tggttcaaat tcaaccatta ttgcaccagt agaacttggt gacaattccc tcgttggtgc
     9361 tggttcaact attactaaag acgtgccagc agatgctatt gctattggtc gcggtcgtca
     9421 gatcaataaa gacgaatatg caacacgtct tcctcatcat cctaagaacc agtaggagcc
     9481 tatcatggag tttgaagaaa aaacgcttag ccgaaaagaa atctatcaag gaccaatatt
     9541 taaactggtc caagatcagg ttgaattacc agaaggcaag ggaactgccc gacgggattt
     9601 gattttccac aatggggctg tctgtgtttt agcagtaacg gatgaacaaa aacttatctt
     9661 ggtcaagcag taccgcaaag ctatcgaggc tgtctcttac gaaattccag ccggaaaatt
     9721 ggaagtagga gaaaacacag cccctgtggc agctgccctt cgtgaattag aggaagaaac
     9781 agcctataca gggaaattag aactcttgta cgatttttat tcagctattg gcttttgtaa
     9841 tgagaagtta aaactatatt tagcaagcga tttgacaaaa gtggaaaatc cgcgtccgca
     9901 ggatgaggat gaaaccttgg aagtccttga agtgagctta gaagaagcga aagaattaat
     9961 ccaatcaggt catatctgtg atgccaagac aattatggct gttcagtatt gggagttgca
    10021 gaaaaaatag aggaggtcag tatgggtaaa tctttattaa cggatgaaat gattgaaaga
    10081 gctaatagag gcgaaaaaat ttcaggtcct cctttgctag atgataatga ggaaactaag
    10141 attttaccaa cctcttcttc ccgttttggt tatgccaatc ctaaggatca tggttttagc
    10201 caggaaacct tgaagattca ggtcgaacca tctattcata aaagccgtcg tattgaaaat
    10261 accaagagaa atgtcttcaa ttctaagttg aataaaatct tatttgcggt catctttctc
    10321 ttgattttgc ttgttttagc aatgaaactt ttgtaataga aaaggaattg aaatgaaaat
    10381 aggaattatt gctgctatgc cagaagaact ggcttatctg gtccagcatt tagataatac
    10441 ccaggagcaa gttgttttgg ggaataccta tcatacagga accattgctt ctcatgaagt
    10501 cgttcttgta gaaagtggaa ttggtaaggt catgtctgct atgagtgtgg cgattttggc
    10561 tgatcatttc caggtggatg cccttattaa tacgggttca gctggggcag tagcagaagg
    10621 tatcgctgtt ggggatgtcg tgattgctga caaattagcc tatcatgacg tggatgtcac
    10681 agcttttggc tatgcttatg gacaaatggc gcaacaaccg ctttatttcg aatcagacaa
    10741 aacctttgtt gctcaaatcc aagagagttt atctcaattg gaccaaaact ggcatcttgg
    10801 tttgattgct acaggagata gttttgttgc aggaaatgac aagatagaag cgattaagtc
    10861 ccatttccca gaagttttag ccgtggagat ggagggggca gctattgctc aagcagcgca
    10921 tgccctcaat ctcccagtct tagtcatcag agctatgagt gacaatgcca accatgaagc
    10981 aaacatcttt tttgatgagt ttattatcga agctggacgt cgctctgccc aagtcttgtt
    11041 gacctttttg aaggctttag attaagcgga aatttgacag tttttctagc ttatgataag
    11101 atttaagtaa agaaaagcta gaaaacgttt cagaggatat tatgagtatt gaaatgaccg
    11161 tcagtgagat tgcagaggtc ttaggattat ctcgccaagc aatcaataac cgtgtcaaag
    11221 aattaccaga agaagacaca gataaaaatg acaaaggggt aacagttgtt accagaagtg
    11281 gcttgattaa gctagaagaa atctataaaa aaacgatttt tgaagatgag cctgtcagtg
    11341 aagatgtcaa acaacgtgaa ctgatggaga ttctagtgga tgagaagaat gcagaaattc
    11401 ttcgtcttta tgaacaatta aaagccaagg atcgtcagtt atcagaaaaa gacgagcaga
    11461 tgcgtatcaa agaccgtcag attgctgaga aagacaaaca attagatcag caacaacaat
    11521 tgacccttca agccatgaag gatcaagaaa atcttaagct agagctggac caagcaaaag
    11581 aagaagtcca atccactaag aaaggctttt ttgctcgttt atttggagga taatcaagga
    11641 gctgtttagg ttaaatgcta acctgatggc ttcttttgtt tgtgaatagt atagttgatt
    11701 agaacagaga cagtagagga gaggtacaga atggaacgat tagaagatta cattgctttt
    11761 gacttagaat ttaatcagca cgaaggacaa acacacttga ttcaagtatc ggcagtgcgt
    11821 tttagagatg gtcgggaaat cgatgcctat gattcctatg ttcataccag cgtccctcta
    11881 aaaagcttta tcaatggttt gaccgggatt acagctgaga ccttaaaaga tgctcctcca
    11941 gttgagaaag tcttagaaga attccaagag tttgtgggtt ctttgctgat gataggctac
    12001 aatgctgcta aaagtgattt acctatttta gcagagcatg gtttagacta tagccaacag
    12061 tacaaggtgg atttgtatga tgaagctttt gaacgtcgga gttcagattt acatggtatt
    12121 gccaatctca aattgcaaac tgttgcctca tttcttggat ttcaaggtca gtctcataat
    12181 agtttagagg atgctcggat gacggcgcgt gtttacgaat ccttcttaga gtcggatcaa
    12241 gctagggaat tgttagagac agaaagtagc ttatcaaaca atccttttgg tgtcttggac
    12301 ttgtctcaat tatttgacta ggagtgccta tgaaacctga aaaacctaaa aaattgggtc
    12361 ttaaacagat ttacctcaat cttgataaaa tcctctttct atttttcttg atttttatga
    12421 tagtggagta tttgtgggta ccattcaatt catggttggc tagcttctta ttagatcaga
    12481 caggttatct ttttatttcc tataacaatg tcttagccat ccttactcat tctccattga
    12541 ttagtctagc ttttttagcc ttgattgtaa ttaaccttct ggttgcttat tttcaaatag
    12601 gactcctctt tattggtgct cgtcatctcc tctataatga aaagcgaagg ctaattgagt
    12661 atatccgcaa ggttttccat gaaagttttg gatttatgaa aaagctaact tgcgctaaag
    12721 ccttgtttat ctttttctat atagctatgc tttttccttt tatacggaaa atgctgaaga
    12781 tttattatct taataaaatc gtcattccag agtttatcca agcttattta gaagataaat
    12841 attggatgtg gtggctgagc atcctacttc tatctcttat ctttctctat gtctctgtca
    12901 gattgatgtt tgcccttccg aagattgtat atgatcagct gactgttcga gaagcagtga
    12961 tgtttagctt ggagaaaacc aagaagagag ttacttttta tgcttggaat ttattttaca
    13021 tcttgctcaa agcacattta ttattttatc ttcctttgat tcctttatta ttggctcaaa
    13081 ctttggtaga tgatatcact cagagagagt ctctgattct tggtattttg aattttgtcg
    13141 tgattaaaaa tctctattat atggctctga cttatttttt ggttaaattt gtttcatttt
    13201 tgacaggaaa ggagctggat atgcttccta ggagagaaaa agatcacatc gtgcgatggg
    13261 gtgtcatgac ttgtgccagt cttttctttg ccttagaagg ttatatttat ctggaggctc
    13321 ccatggttca tctacctcaa cttatttctc accgaggggt ttccaatgca aatgggattc
    13381 aaaatacagt agagtccttg gaaactacag cacaactcaa accagacttg gtggagacgg
    13441 acgtgcagga aacaaaagat gggcagtttg tcatgatgca tgatgctaac ttgaaaaatc
    13501 tagcaggtat caataaaagt cctcaagact taaacttgga ggagcttaaa gggattgata
    13561 tttttgaaaa tggctaccag actaaaattt caagctttga agattatctc agtcgagcca
    13621 acgaacttgg tcaaaaatta ctaattgaaa ttaaaaccag taaaaaagat agtccagaca
    13681 tgatgaaccg ctttttagcc cgttatgctg caaagctcaa gatttatgga catcaaatcc
    13741 agtctttaga ctaccatgtt gtcgaaaaag taagacagta tgatgccgaa ctgccagttt
    13801 atttcatcat gccctacaat tctgtctttc ctaaaacaag agcgacagga tatactatgg
    13861 agtactcaac cttggatgaa tattttgtaa gcaaactatg gacaacggat cagaaacttt
    13921 atgtctggac tgtcaatgat tcagaagcta tcagcaaatc tcttcactta ggagtagatg
    13981 gagtgattac agatgatttg gaaaaagttc agcgggaaat agaagtagcc caagaagatc
    14041 cagaatatac ggatttgctc ttgaaaaaag cattggaatt ctttgaattt tagtaataaa
    14101 aaagaggaac ggttgttcct cccatgatag gtaagtattc ttgtgaatgc ttgctttttt
    14161 gtggttgcaa tccgtttacg agaattgttt gtattgttct tgtttcaatt tactttatag
    14221 tgctttgaag ttggaatgct acactataac ttctaaaata tagtaaaatg aaccaaaaat
    14281 agtacacaat gtggtataat cttcttatgg catattcaat agattttcgt aaaaaagttc
    14341 tctcttattg tgagcgaaca ggtagtataa cagaagcatc atacgttttc caaatctcac
    14401 gtaatactat ttatggctgg ttaaagctaa aagagaaaac aggagagcta aaccaccaag
    14461 taaaaggaac aaaacc
//
LOCUS       DAVBCV010000050        14429 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBCV010000050 DAVBCV010000000
VERSION     DAVBCV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14429
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.050"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            136..792
                     /locus_tag="VQ516_001590"
     CDS             136..792
                     /locus_tag="VQ516_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2512003.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            782..1105
                     /locus_tag="VQ516_001591"
     CDS             782..1105
                     /locus_tag="VQ516_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET2512004.1"
                     /translation="MVSKYLLLAVIFSDLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            1209..2039
                     /locus_tag="VQ516_001592"
     CDS             1209..2039
                     /locus_tag="VQ516_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2512005.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKISDKITVETVIKNQGLDNLKVI
                     DLPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2080..2157
                     /locus_tag="VQ516_001593"
                     /pseudo
     CDS             2080..2157
                     /locus_tag="VQ516_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            2261..3091
                     /locus_tag="VQ516_001594"
     CDS             2261..3091
                     /locus_tag="VQ516_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2512006.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            3245..4099
                     /locus_tag="VQ516_001595"
     CDS             3245..4099
                     /locus_tag="VQ516_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2512007.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            4199..5572
                     /locus_tag="VQ516_001596"
     CDS             4199..5572
                     /locus_tag="VQ516_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2512008.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            5565..6626
                     /locus_tag="VQ516_001597"
     CDS             5565..6626
                     /locus_tag="VQ516_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2512009.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            6628..7320
                     /locus_tag="VQ516_001598"
     CDS             6628..7320
                     /locus_tag="VQ516_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2512010.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(7350..7919)
                     /locus_tag="VQ516_001599"
     CDS             complement(7350..7919)
                     /locus_tag="VQ516_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2512011.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            8054..8668
                     /locus_tag="VQ516_001600"
     CDS             8054..8668
                     /locus_tag="VQ516_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2512012.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            8670..10316
                     /locus_tag="VQ516_001601"
     CDS             8670..10316
                     /locus_tag="VQ516_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2512013.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHRASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            10328..11614
                     /locus_tag="VQ516_001602"
     CDS             10328..11614
                     /locus_tag="VQ516_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2512014.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGCD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            11675..12208
                     /locus_tag="VQ516_001603"
     CDS             11675..12208
                     /locus_tag="VQ516_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2512015.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKASAEEEETEELA"
     gene            12286..12756
                     /gene="nrdI"
                     /locus_tag="VQ516_001604"
     CDS             12286..12756
                     /gene="nrdI"
                     /locus_tag="VQ516_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2512016.1"
                     /translation="MKIISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(13049..14311)
                     /locus_tag="VQ516_001605"
     CDS             complement(13049..14311)
                     /locus_tag="VQ516_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2512017.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFSGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         4272 a         2695 c         3277 g         4185 t
ORIGIN      
        1 gcaacctcaa aacaatgttt tgagcaacct gcggctagtt tcctagtttg ctctttgatt
       61 ttcattgagt attattaaga caatatgagg agaaagatga aagaaaaagg attttaggag
      121 ggggcgcagg cagccatgcc aacggctctt ggctatgtca gtatcggcct ggcctgtggg
      181 actatcggtg cgccctatgt gacacctgtt gagatgggct tgatgagtct ctttgtttat
      241 gctgggagtg cccagtttgc catgttggca ctgattgtgg ttcaagctcc tgtggcagct
      301 attgctatga cggtttttct aatcaacttg cgtctctttt tgttgagttt acacgcatcg
      361 acttatttcc gtcataccag tctttggtac aatatcggta tgtctagtat cttgacagat
      421 gagacctatg gcgttttgat gggtgaattg gcccatacag acaaggtaaa tcctatgtgg
      481 atgcacggaa acaatcttaa cagctatgtg gcttggtttg tggggacagt agtcggaacg
      541 gctctgggtg gcctgctacc aaatccagaa atctttggct tggattttgc cctggttggg
      601 atgtttattg gtatttttgc ttcgcaattt cagattatgc aaagacggat tcctgtccgc
      661 aatctgctca ttatcctagc agttgttgcg gtgtccttct ttttgctctt gacagtggtg
      721 tctcagtcac tagctgttct gtttgcgacg ctacttggtt gtagcatggg ggtggtttta
      781 gatggtcagt aagtatcttt tattagcagt tattttctct gacttggtga cttggattct
      841 ccgtatgatt cccttcatct tagtcaagta taagggcttg cctgcaatcg ttgagcgttt
      901 tttgaagttc ttgcccgttt ccattatctt tgccttgatt ctttcaagcg tagtgacagg
      961 taaggttggg agccttcctc aaattaaatg gctagacttc ttagccgtct ttccaacagc
     1021 ttgggtagcc tttcgctacc gcaatctagt cggaacagtt ctctttggag tagtcttgat
     1081 tgccgtcttg cgtttggtct tttaaatcag ccataaagaa aacctatcac agatatatat
     1141 atcatataat ggtgtaaatg atttttttct gttaagatta taaggtattc tattttggag
     1201 gaaatgacat gaaaaaaatc gttaaatact catctcttgc tgccctaggg cttgttgctg
     1261 caggtgtgct agcagcttgc tccggtggtg ctaagaaaga aggacaagca gctagcaaga
     1321 aagaaattat tgttgcaact aattcttcac caaaaccatt caactatgaa gaaaatggcg
     1381 aattgactgg ttacgagatt gaagtcgttc gcgctatctt taaagactct gataagtatg
     1441 atgtcaagtt tgagaagaca gagtggtcag gtgtctttgc gggtcttgac gctgaccgtt
     1501 accaaatggc tgttaacaac atcagctaca ctaaagaacg tgcggagaaa tacctttatg
     1561 cagccccaac tgttaaagac cctaacgttc ttgtagtcaa gaaagacgat tctagcatca
     1621 aatcgcttga tgatgttggt ggaaaatcaa cagaagttgt tcaaggaaca acatctgcta
     1681 aacagttaga agactacaac aaacaacact cagataatcc aactatcctt aactatacta
     1741 aagcaaactt acaacaaatc atgggccact tgagcgatgg tcagtttgac tacaagattt
     1801 ctgataaaat cactgttgaa acagtcatca agaaccaagg tttggacaac ttgaaagtca
     1861 tcgatcttcc aagcgaccaa caaccttacg tttacccact tcttgctaaa ggtcaagatg
     1921 agttgaaaac atttgtagac aaacgtatcc aagaactcta caaagacgga actcttgaaa
     1981 aattgtctaa acaattcttc ggagacactt acctaccggc agaagctgat attaaataat
     2041 ttcttgaaat catccattct gagtaagatg ggtgattttt cagttcgcta ccgcaatcta
     2101 gtcggaacag ttctctttgg agtggtcttg attgccatct tgcgtttggt cttttaaatt
     2161 acccataaaa aaaacttatc acagagatag atatcatata atggcgtaaa tgctcttttt
     2221 ctgttaagat tataaggtat tctattttgg aggaaatgac atgaaaaaaa tcgttaaata
     2281 ctcatctctt gcagcccttg ctcttgttgc tgcaggtgtg cttgcggctt gctcaggtgg
     2341 tgctaagaaa gaaggacaag cagctagcaa gaaagaaatc atcgttgcaa ccaatggatc
     2401 accaaagcca tttatctatg aagaaaatgg cgaattgact ggttatgaga ttgaagtcgt
     2461 tcgcgctatc tttaaagatt ctgacaaata taatgtcaag tttgaaaaga cagaatggtc
     2521 aggtgtcttt gctggtcttg atgctgaccg ttacaatatg gctgtcaaca atcttagcta
     2581 cactaaagaa cgtgcggaga aatacctcta tgccgcacca attgcccaaa atcctaatgt
     2641 ccttgtagtg aagaaagatg actctagtat caagtctctc gatgatatcg gtggaaaatc
     2701 gacggaagtc gttcaagcca ctacatcagc taagcagtta gaagcataca acgctgaaca
     2761 cacggacaac ccaactatcc ttaactatac taaggcagat ttccaacaaa tcatggtacg
     2821 tttgagcgat ggacaatttg actacaagat ttttgataaa atcggtgttg aaacagtgat
     2881 caagaaccaa ggtttggaca acttgaaagt tatcgaactt ccaagcgacc aacaaccgta
     2941 cgtttaccca cttcttgctc agggtcaaga tgagttgaaa tcatttgtag acaaacgcat
     3001 taaagaactc tacaaagacg gaacgcttga aaaattgtct aaacaattct tcggagacac
     3061 ttatctaccg gcagaagctg atattaaata atttcttgaa atcatccatt ctgagtaaga
     3121 tgggtggttt ctcagttttt agggatatag ttttaaacta tatatctgac tatctaataa
     3181 caatttgtga aatcaaacaa attgtgagat actagtacgg tattattttt aaggagaaag
     3241 aatcatgaaa atcaaaaaat ggcttggcct agcagccctt gctacagtcg caggtttggc
     3301 tcttgcagct tgcggaaact cagaaaagaa agcagacaat gcaacaacga tcaaaatcgc
     3361 aactgttaac cgtagcggtt ctgaagaaaa acgttgggac aaaatccaag aattggttaa
     3421 aaaagacgga attaccttgg aatttacaga gttcacagac tactcacaac caaacaaagc
     3481 aactgctgat ggcgaagtag atttgaacgc tttccaacac tataacttct tgaacaactg
     3541 gaacaaagaa aacggaaaag accttgtagc gattgcagat acttacatct ctccaatccg
     3601 cctttactca ggtttgaatg gaagtgccaa caagtacact aaagtagaag acatcccagc
     3661 aaacggagaa atcgctgtac cgaatgacgc tacaaacgaa agccgtgcgc tttacttgct
     3721 tcaatcagct ggcttgatta aattggatgt ttctggaact gctcttgcaa cagttgccaa
     3781 catcaaagaa aatccaaaga acttgaaaat cactgaattg gacgctagcc aaacagctcg
     3841 ttcattgtca tcagttgacg ctgccgttgt aaacaatacc ttcgttacag aagcaaaatt
     3901 ggactacaag aaagcacttt tcaaagaaca agctgatgaa aactcaaaac aatggtacaa
     3961 catcattgtt gcgaaaaaag attgggaaac atcacctaag gctgatgcta tcaagaaagt
     4021 aatcgcagct taccacacag atgacgtgaa aaaagttatc gaagaatcat cagatggttt
     4081 ggatcaacca gtttggtaat aagaaacagg gaggtgggag agaaaattcc acctcttgct
     4141 tttgtataga gtatagattg taaagaagac tattcgttca tagaaaggta gagagaatat
     4201 ggtttttcct agcgaacaag aacagattga aaaatttgaa aaggatcatg tagtccaaca
     4261 ttattttgag gttttgcgta ccttgatttc taagaaatca gtctttgccc agcaggttgg
     4321 actcaaggaa gtcgcaaatt atctgggtga gattttcaag cgtgttggag ctgaagtgga
     4381 gattgatgag agctatacag cgccctttgt catggcacat ttcaagagtt cgcgtccaga
     4441 tgccaagacc ttgattttct ataaccacta tgacactgtg ccagcggatg gggatcaggt
     4501 ctggacagag gatcctttta cgctttcggt ccgcaatggc ttcatgtatg ggcgtggggt
     4561 tgatgacgac aagggtcata tcacagctcg cttgagtgct ttgagaaaat atatgcagca
     4621 ccatgatgat ttacctgtca atatcagctt tatcatggag ggagcggagg aatcggcttc
     4681 aacagaccta gataagtatt tggaaaagca tgcagacaaa ctccgtgggg cggatttgtt
     4741 ggtctgggaa caagggacca aaaatgcctt ggaacagctg gaaatttctg gtggcaataa
     4801 ggggattgtg acctttgatg ccaaggtaaa aagcgctgat gtggatatcc actcgagtta
     4861 tggtggtgtt gtggaatcag ctccttggta tctcctccaa gccttacagt ctcttcgtgc
     4921 tgcggatggc cgtatcttgg ttgaaggctt gtacgaagaa gtacaagagc ccaatgaacg
     4981 agaaatggcc ttgctagaaa cttatggtca acgaaaccca gaggaagtta gtcggattta
     5041 tggattggag ttgcctctct tacaggagga gcggatggcc tttctaaaac gtttcttttt
     5101 cgagccagcg cttaatatcg aaggaatcca gtctggttat caaggtcagg gtgttaagac
     5161 tattttacct gcagaagcca gtgccaagct agaggttcgt ctggttccgg gcctagaacc
     5221 gcatgatgtt ctggaaaaaa ttcggaaaca actagacaaa aatggctttg ataaggtaga
     5281 attatactat accttgggag agatgagcta tcgaagcgat atgagcgcac cagccattct
     5341 caatgtgatc gagttggcca agaaattcta tccacagggc gtttcagtct tgccgacgac
     5401 agcggggaca ggacctatgc atacggtctt tgatgcccta gaggtaccaa tggttgcatt
     5461 tggtctagga aatgccaata gccgagacca cggtggagat gaaaatgtgc gaatcgctga
     5521 ttattacacc catatcgaat tagtagagga gctgattaga agctatgagt agagatatta
     5581 tcaagttaga tcagatcgat gtgacttttc accaaaagaa gagaaccatc acagcggtta
     5641 aggatgtgac cattcacatc caagaagggg atatctacgg aatcgttgga tattctggag
     5701 cagggaaatc aacccttgta cgggtgatta acctcttgca aaaaccatct gcagggaaaa
     5761 ttaccattga cgacgatgtg atttttgatg gtaaggtcac cttgacggca gagcagttgc
     5821 gtcgtaaacg tcaagatatc gggatgattt tccagcattt taacctgatg agccaaaaga
     5881 cagcagagga gaatgtagcc tttgccctta aacactctgg actcagcaag gaagaaaaga
     5941 aggctaaagt agctaagttg ttggacttgg ttggtttggc agatcgtgct gaaaactacc
     6001 cttcacaact atctggaggg caaaaacagc gtgtggcaat tgcgcgtgcc ttggccaatg
     6061 atccaaaaat cttgatttca gacgagtcaa cttctgccct tgatccgaag acaaccaagc
     6121 agattttggc cttgttgcaa gatttgaacc aaaaattagg cttgactgtt gtcttgatta
     6181 cgcatgaaat gcagattgtc aaagacattg ccaaccgtgt tgcagttatg caggatgggc
     6241 atttgattga agagggtagt gtgcttgaaa tcttctcaaa tcctaaacaa cctttgactc
     6301 aagactttat ctcaacagct acaggtattg acgaagccat ggtcaaaatc gagaagcaag
     6361 aaatcgtgga acacttgtct gaaaacagtc tcttggtgca actcaagtac gctggagctt
     6421 caacagacga gccacttttg aatgaattgt acaagcatta ccaagtaatg gctaatattc
     6481 tctatgggaa tatcgaaatt ctcgatggta ctcctgttgg agaattggtg gtggtcttgt
     6541 caggtgaaaa agcagcgttg gcaggtgccc aagaagccat tcgtcaagca ggtgtacaac
     6601 taaaagtatt gaagggagga cagtaagatg gaatcattga ttcaaaccta tttaccaaat
     6661 gtctataaaa tgggttgggc tggtcaggca ggctggggaa cggctatcta cttaactctt
     6721 tatatgacag ttctttcctt cattatcgga ggcttcttgg ggctagtggc aggtctcttt
     6781 ctcgtcttga cagcgccagg tggtgtcttg gagaataaag tcgtattctg gattttagac
     6841 aaaattacct caatttttcg tgcagttccc tttatcatcc tcttggcaat cttgtcacca
     6901 ctttctcact tgattgttaa aacaagtatc gggccaaatg cagcccttgt cccactttct
     6961 tttgcagtct ttgccttctt tgcccgtcag gtgcaggttg tcttggctga attggatggc
     7021 ggtgtcattg aggcggctca agcgagcgga gcgactttct gggacatcgt gggtgtttac
     7081 ctatcagaag gtcttccaga tttgatccgt gtgacgactg tgaccttgat ttcccttgtt
     7141 ggggaaacag ctatggccgg tgcggttgga gctggtggta tcggtaacgt agccatcgct
     7201 tatggattta accgctacaa tcacgatgtg accatcttgg caaccatcat tatcattttg
     7261 attatctttg caatccaatt cttgggagat ttcttgacta agaaattgag ccataaataa
     7321 aaaagagccg cgtggctctt tttaactgat cagattttct gtgcaaattt tttactcaag
     7381 gcttgtccaa tcaaggcacc cactagggct ccgatgacaa tacttgcgat aaatagaagg
     7441 acagttccag ggtttggagc gaccatgatg cggtcgatat attcttggga ttttcctctt
     7501 gccagaagag tagccatata ggctttgggc gcaatccaca taagcaagat tggtcctgtt
     7561 gtactaaagg cgaaaataat gaaagaaagg aagttctttg ttttgtcctt gtattttcct
     7621 aaatgagcta ctccatctgc taggaggcca cagataattc caggaaggaa ggaaccagca
     7681 ccgtgtttag ttcccaagaa aaagagggca atgacaaggc cgatagtggt aatggctcca
     7741 aagcgcggaa cttttgcgac taggatcata tagacgctac cgccgacaag ggcagtaaag
     7801 gcaggcgcat aaaacatgtt tccagtttgg tcaaagagat tgcccaaaag gacaccaatc
     7861 cccatgcaga ggaagtaaag aactgcaaaa agcagtgtag ttaagataga tttcttcatg
     7921 aattgtctcc tgataatttt ttcacaattc tcattttacc atggtttggt gggattgtaa
     7981 atggatacta gaagtttttg aaaacgcttg aaatatgata taatggtttc atagttattt
     8041 ttaggggaat atcatgggta gatttttaga ctttgtcttt aatcgtttct ttttagggat
     8101 gattgcgaca gccttctttt ggctattaac tttagcagga gggattatcc ttggtctagc
     8161 gccggctagt gccaccttga tgagcttata tgcagaacat ggttatagct ttcgggaata
     8221 cagtttgaag gaggcttggt ctctttacaa gcaaaatttt gtctcaagca acctgatttt
     8281 ctatagcttt ttaggtgtgg gtctagtttt gacctatggt ttgtatctct tggtgcaatt
     8341 gcctcatcag actattgttc atttgattgc gacccttttg aatgtcctag tagttgccct
     8401 gatctttttg gcttatacag tatctttaaa attacaagtt tattttgcct tgtcctatcg
     8461 aaatagtctc aaattatcct tgattggcat ctttatgagt ctagcagctg tggccaaggt
     8521 tctccttggg actgtgctac ttgtagcaat tggttactat atgcctgccc tgctattttt
     8581 tgtaggaatt gggatgtggc atttctttat cagtgatatg ttggaacctg tctatgaaat
     8641 catccatgaa aaattggcga caaaatagaa tgaagcagtt ttggctacat acgcttctaa
     8701 gaacctatag ttcagtgatg atcattatca ttgcgagttt tgcaatctta ctctcttacg
     8761 ctgactggga ttcacgtgaa aaggaagccc agagagtagc ccagcgtgta actgctagaa
     8821 cagtgagtga aattgaatat taccatagag agtcaaccca gatagctcag gctttagttg
     8881 aaaatcaagc tcgtattgag ggaatctata aatactttag ccttagcatg ccagactatt
     8941 tttactggca attagagcgg aaagcttcgc cttatatatc agtctctctg tatgaaaatg
     9001 ttgatgacct ctatgttcga aatgattttg taactggggt ggccattgct tttcaagatt
     9061 acaaggaagt ctatgtttct actaaagaca aacgtagtgg agaaaaaatc agggctgagg
     9121 atttcaaacc agcaggaaat agttttgcca ttccggtgtc agatccagtg tcagatcaag
     9181 atttaggagt gatttacatc tccttggatc ctgctgtttt ataccatgcc attgataata
     9241 ctagaggtca tactccgatg gcagtaacag tgacctcacc ttttgatacg gagatttttc
     9301 atattggtga gacagttgat agggagagtg aaaattggct agttggctta acttctcatg
     9361 gatatcaggt tcaggtggca gttcctaaaa actttgtttt acaaggaaca gtgactagct
     9421 ctgctttgat tgtgggtttg agccttctct ttattgtcat tctttatctg actttgaggc
     9481 agacttttgc taattaccaa aagcaggtag tggatttagt ggaatccatt caagtcattg
     9541 ctcaaggcga agaggggcgt cggattgaca tttccgaaaa agatcaggaa ttactcctaa
     9601 tcgcggagac gaccaatgat atgttggatc gattggaaaa gaatatccat gatatttacc
     9661 agttagagct tagtcaaaaa gatgccaata tgcgagcctt gcaggcgcaa atcaatcctc
     9721 attttatgta taatacgctg gagttcttgc gcatgtatgc agttatgcag agtcaagatg
     9781 agttggcaga tatcatttat gaattcagta gtctcttgcg taacaatatt tccgacgaaa
     9841 gagagaccct cctcaaacag gaattagaat tttgccgtaa atacagctat ctctgcatgg
     9901 ttcgctatcc caagtccatt gcctatggtt tcaagataga tccagagtta gagaatatga
     9961 agattcccaa gtttaccttg caaccgctgg tagaaaacta tttcgcgcat ggtgttgacc
    10021 acaggcggac agataatgtg attagcatca aggctcttaa acaggatggt tttgtggaaa
    10081 ttttggtggt cgataatggt agaggaatgt cggctgaaaa gttggcaaat atccgagaaa
    10141 aattaagtca gaaatatttt gaacaccgag ccagctacag tgatcaaagg cagtctatcg
    10201 ggattgtcaa tgtacacgag cgttttgtgc tctattttgg agaccgctat gccattacga
    10261 tagagtctgc agagcaagcc ggtgttcagt atcgtattac aattcaagat gagtagaaag
    10321 ggagaaaatg tataaagtat tattagtaga tgatgagtac atggtgacag aaggtctgaa
    10381 gcgtttgatt ccctttgata agtgggatat ggaggtcgtc gcaacagcca gtcatgccga
    10441 tgaagctcta gaatatgttc aggaaaatcc tgtcgatgtc atcatttccg atgtcaatat
    10501 gccagacaaa acagggcttg atatgattcg ggagatgaaa gagatcttac cagatgctgc
    10561 ctatatcctg ctctcaggtt atcaggagtt tgattatgta aaaagagcaa tgaaccttag
    10621 tgtggtggac tatttggtca agcctgttga taaggtagag ctgggaaatc tgctggagaa
    10681 gattgcaggt cagctcggcg agagagggaa gaaaagtcag actcttagtc aagaattaga
    10741 cgaggctgga tttgttagtt atttagggga taaggagaat tggtggatag gtctatccaa
    10801 ggaaaaacaa ggttccttca ccattcccta ctatgtcttg ggccaagact ggcagatttt
    10861 catttctgac caacccctag atggtttagt cgttacacct tttgaagctc cttatcaaga
    10921 acactttgaa cgctggaagc tgaatgctga gaaaaccctc ttttacggtt ctgtaaattt
    10981 gcagcagtct gagagtctct ttgcctatta cgaaccgatt tatagggtta tcattcaggg
    11041 aaatctcaat caaatcgtag aagagttaaa tctcttggag aaggtagttc ttgaaaatac
    11101 gccgcgtgtt ccgattacta aacagctttt tattcagttt gtcatggatg ttttccattt
    11161 atttgaacat ctcaaagctg atgacctgac agacatcgtc aaaaccattc atgctattca
    11221 atccttcgat gaattggttc cttatatcaa ggaaactctg agcagctttt tcggtcaata
    11281 ccgtatgaat gaaaatgtgg tcagtgtgct ggaagtcatt ggttgtgatt accaaaaaga
    11341 gctttccctc aaggatatca gtaaggctct ctttatcaat cctgtctatc tagggcagtt
    11401 gattaagcgt gaaaccgatt cgacttttgc agagttacta aacaaacaac gtattaaggc
    11461 tgcccaacaa cttttgcttt caactagtga cagcatcgaa gatatttgtt atgctgttgg
    11521 ttacagtaac cttggatatt tctataaagt tttccgaaaa ttgtgcggaa aatcgccaaa
    11581 agcctaccga aaacaggtag aaactatact ataagatttg tattccttta caaaatgtgc
    11641 tataatataa atattattat taggaggttc tatcatgaaa aagaaaccaa tttatctatg
    11701 ggtcttgcta atcttgtctg cccttatttc agttccgtct ctgtttggaa ttgtgagtcc
    11761 cttgcctagt aaagaagccc ttcgtactgc tcagaaacaa gttgcagggg tcaatgctca
    11821 gcaattagaa gaccagctta attataccta tagagtagca gaagcatctc attctatttt
    11881 taatgttgct ttgattgtgc tatctactat tttagtggtc gtagcgattg tcttccttgt
    11941 tcgtaaaaat ttgcaatatg caaactatac ttacgttggc tatgttttgt tagctattat
    12001 tggttcgatt tatggctatg ttggtttaca agacgctgtg cagttggttc aagatgagag
    12061 catgcgtttg acagtgagta ttggttcaaa agctgttagc attttctata tcgtcatcaa
    12121 tgttctcttc ctagcccttg tcttctataa gatgtggcga caacagaaag cctcggcaga
    12181 agaagaggaa acagaagagc ttgcctaagt attgacaaaa aagaactatc aagatacaat
    12241 cttggtagtt gttttttgat tatagataaa atcatggagg tacatatgaa gataatttct
    12301 ttagtttata ttagtttgag tggcaacact gaaagttttg tgacgcgctt gaaagactat
    12361 ctcttgtccc agtacaaagg gattgaggtt caaaagattc atatcaagga tttagtcaag
    12421 gaaggcaaaa atttctatga aatggaccat ccctatgtcg cttttttgcc gacctatctc
    12481 gaaggtggga atggcgtgga taacggagat gttgagattt tgacgacacc ggtgggagat
    12541 tttattgcct atggtaacaa tgctagtaag tgttttggtg tggttgggtc aggaaatcgt
    12601 aactttaata accaatactg cctaacagcc aagcaataca gtcaacgctt tggctttcct
    12661 gtattggctg actttgaaat gcgaggcatg ctggaagata tcaaacatgt cgcagctatt
    12721 atcgcagatt tgtatgagtt ggaaaaggag aattaatact caatgaaaat caaagagcaa
    12781 actaggaagc tagtcgcagg ttgctcaaag cacagctttg aggttgcaga tagagctgac
    12841 gtggtttgaa gagattctcg aatagtataa aattcttata agaggtggtt gaagttgttc
    12901 aactgttttt tgagtataaa cagtctttaa gaacgtaaaa aagcataagt tcaggtcttc
    12961 aaaaccttgt tcttatgctt tttatcatag aaatatttgc tgaattttct cttgaaatga
    13021 aaccttatct tcatctcgcg cttgtccctt accctgcttt tccgattgcg agtgcgtaga
    13081 taaagaagat ggcgaagcca aagaggaaaa tcaatcccgc aatggcaagg tgtttgagcc
    13141 aagaaggaag atttttgttt tcacgcgtta ccaaggcgta attcaaaaga gcaaagaaag
    13201 gtgttgtcag gaaagagcca atcatggcaa agcggagcat ggttgaaacc tgaccagaga
    13261 agaacttgat aatgacgata ccgatgatag cagtgatggt catccagatg ttcaaagatt
    13321 tacgattgtc ctctttttga ctgattagca gtcggagaga ttcctgatta acgcgagaat
    13381 agccatcgat aacagttata actgttccaa agatacagag gaaggcaata aaggtaatca
    13441 agtaacggga ccattcgcca agaacagagg catacatgcc cacgaattga gagatgtatt
    13501 tggctgaagc agcttcaacc gcctgccctg taggatactg aatcagtgct cccagtgcca
    13561 caaagaagac ggctaggata gctgttccaa tataaccagt gttaaagtca aacagagcgt
    13621 cttctgtgtt aaagttgacg gtctttctct tttcagctga ccaaagtgaa ttgatggctg
    13681 aaatttcaat aggagccggc atccatccta ggagggagac gatgaagggc agagctgcca
    13741 tttgccaagg tgtcttctcg acaaaatcag aactgtattc tggatgcttg accgccgcaa
    13801 tgataactgc cagaacagtc gcaatggtta aggcagacat aatccatttg accatgccgt
    13861 ctaaaagttt gtagcctcca aagagtagca tagcccaaat gattgcaacg agaatgaggg
    13921 accactgagt aatgctaagt ccaatcattg ggaaggcact ggcgatgata gctgagcaca
    13981 gaatggcaac accagccgtg ttgaccatag ccgaaaagac attgaggata aagaaaatcc
    14041 agagatagag ttttcctttt tcggcataac cttcaaccaa agtctttcca gtatcagctg
    14101 tgtattcagc accaaaacgg aaaaatggat atttaaagac attggctaag atgaccaaga
    14161 gaagtagaga ccaaccgtaa gaaccgccag cttgagttga ggatacaatg tgggaacctc
    14221 caacagcggc agttgccatt aggattccag gtcccattgc ttttagcttg cttgcccagg
    14281 ttgattgatt taaggaaata gcttgcgaca tgataaatac tcctttttga attttcagaa
    14341 taatctgtat atgtaatcta tattctacaa aaaattttgg aaaaatgcaa gaatattttg
    14401 aaaaaagata gaaaatttac aaagaaaat
//
LOCUS       DAVBCV010000051        14315 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBCV010000051 DAVBCV010000000
VERSION     DAVBCV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.051"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..1288)
                     /locus_tag="VQ516_001606"
     CDS             complement(<1..1288)
                     /locus_tag="VQ516_001606"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2512018.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1366..2613)
                     /locus_tag="VQ516_001607"
     CDS             complement(1366..2613)
                     /locus_tag="VQ516_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET2512019.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGVSIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVSVIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="VQ516_001608"
                     /pseudo
     CDS             complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="VQ516_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
     gene            3778..4119
                     /locus_tag="VQ516_001609"
     CDS             3778..4119
                     /locus_tag="VQ516_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2512020.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4177..4257
                     /locus_tag="VQ516_001610"
                     /pseudo
     CDS             4177..4257
                     /locus_tag="VQ516_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4373..6076)
                     /gene="ilvD"
                     /locus_tag="VQ516_001611"
     CDS             complement(4373..6076)
                     /gene="ilvD"
                     /locus_tag="VQ516_001611"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2512021.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6309..7241)
                     /locus_tag="VQ516_001612"
     CDS             complement(6309..7241)
                     /locus_tag="VQ516_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2512022.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7238..8095)
                     /locus_tag="VQ516_001613"
     CDS             complement(7238..8095)
                     /locus_tag="VQ516_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2512023.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(8099..9445)
                     /locus_tag="VQ516_001614"
     CDS             complement(8099..9445)
                     /locus_tag="VQ516_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2512024.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(9458..9742)
                     /locus_tag="VQ516_001615"
     CDS             complement(9458..9742)
                     /locus_tag="VQ516_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2512025.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9746..11776)
                     /locus_tag="VQ516_001616"
     CDS             complement(9746..11776)
                     /locus_tag="VQ516_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2512026.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            11968..12975
                     /locus_tag="VQ516_001617"
     CDS             11968..12975
                     /locus_tag="VQ516_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2512027.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            12978..13178
                     /locus_tag="VQ516_001618"
     CDS             12978..13178
                     /locus_tag="VQ516_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2512028.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13330..13512
                     /gene="rpmF"
                     /locus_tag="VQ516_001619"
     CDS             13330..13512
                     /gene="rpmF"
                     /locus_tag="VQ516_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2512029.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            13528..13677
                     /gene="rpmG"
                     /locus_tag="VQ516_001620"
     CDS             13528..13677
                     /gene="rpmG"
                     /locus_tag="VQ516_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2512030.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14169..>14315)
                     /locus_tag="VQ516_001621"
     CDS             complement(14169..>14315)
                     /locus_tag="VQ516_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2512031.1"
                     /translation="YLNESGSMATGWVTVSGKWYYTYNSGDLLVNTTTPDGYRVNANG
                     EWVG"
BASE COUNT         4616 a         3028 c         2463 g         4208 t
ORIGIN      
        1 attgagtata aaatcctagt ttttcaaaga tttctgagaa gttttggctg attgtctcaa
       61 gtgacacttg cacttcttct cgggtttggt tgttcttgac cgtcacttgt ccgctttcga
      121 cttcgctctc tcctagggtg atgagggtct tagccgcaaa gacatcggct gacttgaact
      181 gagcttttag tttacggttg aggtaatcac gctctgcttt gaaaccttgt tggcgaagag
      241 cctgtaccaa ttccaaggcc ttgatatttg ccccttcgcc caagactgcg atatagacat
      301 ctagggcgtt ttcgataggg agggtcacac cttgcttttc aaggatgaga agcaggcgct
      361 ctacaccaag tccaaaacca aatccagcag tttcagggcc tccaaagtaa gcaaccaaac
      421 catcgtagcg accacccgca cagacggtca ggtcattgcc ctcaatctct gtgataaact
      481 cgaaaatggt gtggttgtag tagtccagac cacgcaccat attggtatcg atgatgtaat
      541 ctactccaag attttccaac atctgacgca cagcatcaaa atgagcttgg ctttcttcat
      601 caagaaagtc caagatagac ggcgcattct ctactgccac cttgtcttct ttttccttag
      661 agtccaagac acgaagagga ttttcctcca agcgacgttg gctatcctta gacaaggtct
      721 ccttgagcgg tgtcaaatag tcaatcaagg cttggcggta ggctgcacgg ctctcaggat
      781 ttccaagagt gttgaggtgc aatttgacac cttgaatacc gatttccttc aaaaaatggg
      841 ctgccatagc gattgtttcc acatcggtag ctggattgct agagccaaaa cactcaacac
      901 caatctggtg gaattggcgc aagcgccctg cctgtggacg ctcataacgg aacataggtc
      961 ccatgtagta gaacttgctt ggcttttgca cttctggggc gaaaagttta ttttccacat
     1021 aggaacggac aacgggtgca gttccttctg gacggagggt aatatgacgg tcacccttgt
     1081 cataaaaatc gtacatttcc ttggttacga tatccgttgt atctccgaca gagcgactga
     1141 taacctcgta atgctcaaaa ataggcgtgc gcacttctgc atagttgtag cgtttgaaaa
     1201 tctcacgggc aaagccctca acgtactgcc acttagcaga ctcagcaggt aaaatatcct
     1261 gcgttccttt tggtttttgt aatttcatag ggaatcctct ttaaacttaa tagtcttatt
     1321 ttaccataaa tagagggatt aaaacagtaa gaaaaaaatt aggatttaga tatcattttt
     1381 gagattaaga attgtcaaaa aaatagctag caaggaaaga ccaacaaata gcatccaagt
     1441 caactgtata ttccatacgg ctactagtga aaaacaagct gttcccacag gtatggataa
     1501 ggtaaacaat agacctaaaa aattactagt acgagctaga acctctggag ctagattttt
     1561 catgagcatg gcactaatct ttggttgaac tttaccagac acatacagag taaagaagag
     1621 aaatagcaaa ccaagcacga cttgattgaa taaattagcc aaaccaacta gactaagtcc
     1681 tacggtctcc cacatcatca atctaggcaa ggactgcttc ccaaaataat cattgcccgt
     1741 aaggctactg atgatgactg atactaaaac acagaattga ttgataaata gtgcctctgt
     1801 ataagaaaaa ttcaagagag aatggctcaa aaagaagata ttataaattc cacccaaagc
     1861 gccacccaag gaattaataa gcaagacagc aaagagcata aaaccaaagt ttttctgtcc
     1921 acttttaaga aaaacgagac gtaaatttcg gtaaattgtt aggaactggt ctttgataga
     1981 aagcttctca ttttttaagt tttcaccatc agcagatgac attgacaggc tcaatttgct
     2041 ttttcctaaa aagaggatag tggctgatac taggaaaaag caggcattga ttcccgcaac
     2101 gagagaaaaa ttgttgaccg atagagctaa gagccagact ccgaaagctt gaccaccaat
     2161 agctgaaata taggtgatga actgtgaaaa agaataagcc tccatcagat catcttcagc
     2221 tactttttcc ttaataagag gcatacgcag gccacctgca aaatcactga tgatatcact
     2281 aatgacattg atcaaacaca ggctagaaaa ggcaaagaga ctagcttgct gaacaactag
     2341 ggctgctaga aaaaatagaa ccgcctgaaa caaaccgcta tagaccatcc atttgacctt
     2401 gtccctcgtg taatctgccc gaatccctgc aaaaactgta aagagggtcg gaagaatcat
     2461 gacaatattc gccatagcaa cagcaaaaga tgcttgtgac aaggtcgatg catagacgat
     2521 aaagaccagg ttgaaaatcg aaacaccaaa agcattgaag aagcgtgata aagtcaaaat
     2581 tctataagct ggatttctaa acaatagttt catagataat ctccctcctt gttctaagtt
     2641 ctacatcaag aatagcttta gtatagcact tatgaatttg cgaaacgatt cttttgaata
     2701 tatgcaacaa acaaaatgag caaaaaaacg atagaacttt tgtaatgacc tgagagaggg
     2761 caacagttct atcgcttcaa attttttctt ggtttgcaga tattcaagaa tcgggagttt
     2821 ttctatagta ttcggcagat ttattacagc caagcatctc aaaaatacgg acagcatcct
     2881 ccatcttttt ctggccttcc ttgactctac cttgcttgct atcaaggaga ccttctgccc
     2941 acagataaac aattcggaaa taggtctcat tttccttgta gaaatgctct tcgataacac
     3001 gtttaaaata ataggcattg gtaaattctt cacactcaat actagctaaa aagccattca
     3061 atagtatagt atgaaaaagg tttcgattgc cagacatttc cattagaaaa tcggatttac
     3121 gtaccatttc tcgtacatat ctagtaaaaa gagaaacaga taaaaatgga gaactgactg
     3181 aaaataaatt gagttcatag attccccaga tctcggtaga aaacaaataa tcatgaagga
     3241 cttttccttc ctctgctgtt aagtctaccc tttcatctat gctcttcata taagacttga
     3301 taataatggc atttagaata tgtttctgtt tgttgtgaga atgggcatgc ttttatactc
     3361 cctgcgatat aagtcctcaa gaggtgctat attctttggt tccaagacat ctgtaatttc
     3421 ttttctcaac tcagaatctg tatcatactg gaaacctctt gccagaaaga ggatctcctc
     3481 cacactggca gatatatttt ccagagcaaa tagaaacttt tccaccgaaa gctcactctg
     3541 acctgtttca aaacgggaca acatagacgg cgaaaattgt cctccggttg cttgtctcag
     3601 tgagatattt cttgactctc gtaattgtct aaagactttt ccaatctgct ccatagactt
     3661 ccccttgatt ccgtattttc ttcattttat catatttttc agaaaattca tcaaaaactt
     3721 gccaaattgt cagaattatg agaaaataga ggatatttat cacgtggagg gactgctatg
     3781 agagacgata tcaaaatcaa tgaccgtgct ttggccttgc aagaccaaat tatcgaaaaa
     3841 ctagagaaag tttttgatac agatgtggaa ttggatgttt acaatctagg tctgatttat
     3901 gaaatcaatc tggatgaaac ggggctctgc aagattgtca tgaccttcac cgatactgcc
     3961 tgtgattgcg ccgaaagcct gcctattgaa atcgtggcag gtctgaaaca aatcgagggt
     4021 atcaaagata tcaaggttga agttacctgg tcgcctgctt ggaaaatcac acgaatcagt
     4081 cgctatggcc gtattgccct tggactacca cctcgttaag cagaccaatc acttttaaag
     4141 atgaaaaaag caagccgaaa atggcttgct ttttgatact caatgaaaat caaagggcaa
     4201 actagaaaac tagccgcagg ttgctcaaaa cactgttttg aagttatgga tagaactgac
     4261 gaagtcagcc caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat
     4321 acctacggca aggcgacgtt gacgtgattt gaagagattt tcgagtatga gtttattttt
     4381 tacctgactt gtccatattc cagaagtctg tcacggctcc gcgtgaagca gatgatacga
     4441 tgtgggcata tttaccgagg acaccacggc tgtaaagtgg tggcaaggtt gtttctgcct
     4501 tgcgtttttc aagttcttct tcggatacgg ccatagaaat ttctttggta tcttggtcaa
     4561 ccgtaacgat atcgccggta cggagatagg caattggtcc accatcctga gcttcaggag
     4621 cgatatgtcc aacaaccaga ccataagtac caccagagaa acgtccgtcc gtcaagaggg
     4681 ccaccttatc tccctgacct ttaccaacaa tcattgaaga aagtgatagc atctcaggca
     4741 taccaggacc acctttaggt ccaacaaaac gaacaacgac tacatcgcca tcaacgattt
     4801 catctgtcag aacggcctga atcgcatctt cttctgagtc aaagacctta gctggcccaa
     4861 cgtgacgacg cactttaaca cctgatacct tggcaactgc accgtcagga gcaaggttcc
     4921 cgttcaagat gataagcgga ccatccgcac gttttggatt ttcaagtggc atgataactt
     4981 tttggcctgg agtcaagtct gcaaagtcag ccaagttttc agctacagtc ttaccagtac
     5041 atgtgatgcg atctccgtga aggaaaccat ttgccaacaa atacttcata accgcaggga
     5101 caccaccgac ttcgtagagg tcttggaaga catactgacc agatggtttc aagtcggcca
     5161 agtgaggcac acgttcttga atcgtattga agtcctcaag tgacaagtca acatttgcgg
     5221 catgggcaat ggcgagcaag tgaagagtgg cgtttgtaga accaccgaga gccatcgtta
     5281 cagtgatagc atcttcaaag gcttcacgag tcaagatatc tgatggtttg agaccaagtt
     5341 ccaacatctt aacaacagca cgtcctgctg cttcgatatc ttctttctta tcagctgatt
     5401 cagctgggtg agaggatgac cctggcaaac tcatccctag aacttcgata gcagttgcca
     5461 tggtattagc agtatacata ccaccacaac caccagggcc agggcaggca ttacattcaa
     5521 gacgtttcac gtcctcagct gtcatgtcac cgtggttcca ttttccgata ccttcaaaga
     5581 cagaaaccaa gtcgatatct ttaccatcaa gatttcccgg tgcaatagtt ccaccatagg
     5641 cgaaaatagc tgggatatcc atattagcaa tagcaatcat agatccaggc atgttcttgt
     5701 cacagccacc gatagcgacg aaggcatcca cgttgtgacc actcatagcc gcctcgatgg
     5761 agtccgcgat gatgtcacga gatgttagag agaaacgcat accaggcgtt cccatagcga
     5821 tcccgtccgc tacggtaatg gttccaaact gtacaggcca agcgcctgca gatttgacac
     5881 cttctttagc cagtttcccg aaatcatgca agtgaatgtt acatggtgta ttttccgccc
     5941 aagtcgaaat cactcccaca atcgatgttt caaagtcctt atctgtcata ccagtcgcac
     6001 gaagcatagc acggttaggt gatttaacca tgctgtcata aatgctactg cggtgacgtt
     6061 tatctaattc agtcatctta tccctcccat ttcagttttt actattatag cacaattttc
     6121 gcatgaagaa cagaataaaa ttcttgaatt ttcagaaaat tctatacaca tgtgaaatat
     6181 ttaaaattaa aaacaacaaa gcggattagt gcactttctg atgaccagaa tatgcttttt
     6241 aatccgcttt ctttaaataa cgtactgtaa tttttacaga aattctttca aataagtgta
     6301 tttaacatct atcttgcatt ataaatttct agaaccttct cttttatatt cgattcactc
     6361 aaaccatact cattaagaag ataatccatt ttccctactt gaccgaatct ttcttgaaca
     6421 cccatccgat gaatttttgt tattccatca tcagagaata attcacataa agcactgcca
     6481 attccaccta tctgattgtg gttttctaca gtaaatatag tttttccact taacattgtt
     6541 tttatctgtt ctggtatcgg tttgattcta aataaatcta tcacacctac tgaataacct
     6601 aatttagaca gttcatctgc aactcgaata cttggagcaa ccattatgcc agaagcaacg
     6661 attacaagat cttcaccatg ccttaactca atgtagcctt tagaaaaatc ttctccacct
     6721 tgatacacag gaactggagc ttttctaatt gttcgaatat attttagtcc ttttaagtct
     6781 aatgtctggt tcaatatttc acgaaattgg atatcatcag ttgcttcgaa aatgattgat
     6841 ttaggaatta aacgtaacaa tccaatttct tcaaatggca tatgtgttcc accattcatc
     6901 tctgccgtta ctcctgcatc tgatccaatc acagtggcat ccaattgtgc gtatccaaga
     6961 gaaataaata attgatcaaa tactcttcgt gaagcaaaag gaccaaatgt atgaagataa
     7021 ggtctaaacc cctgaataga caagcctgct gcaaggccga ccatttctgc ttccataatc
     7081 ccaacattca cataacggtc tccaaagtcc ttttcaagat tattagtagc catcgaactt
     7141 gacaaatcgg cttctaagac tactatatca gaatcacttt gattagcctc taaaaggaag
     7201 tctctatata catgccgtaa ttctttcgta cttctcatca ttctgtttcc tccaattcct
     7261 gacttaatct ttctacaact gaagttaaca tttgtttctc ctctacagta gggcgaagat
     7321 gatgattgga tttcatttct tccagctctt gaaccccttg acctttaata gtatctaata
     7381 caatgcactt aggtgatgaa ttatttgact gttttaattg gacaatccct tcataaattt
     7441 ctctaatatc tgaacccttg accctaatgg attcaaatcc aaatgctgaa aatttttcta
     7501 cgaaatcacc tggattacaa atatcctttg taaaaccatc taattgtttt ttgttatcat
     7561 caacaaatac aattaagttg gataactgtt gatgagaagc aaactgtata gcctcccaac
     7621 attgtccctc atttaactca ccatctccaa caatagcgta agtataaaag ggactctttc
     7681 ttattctctg accatatgca agtccagttg caacactaat tccttgtcct aaagagcccg
     7741 ttgtcatatc tatgcctggc gttagatttc tatcaggatg agacggtaat ttggttccat
     7801 ttgtatttaa agaatataag aattctttgt caaagaaacc attcaaatag agtgtactgt
     7861 atagagctgg tcctccgtga ccttttgata atatgaaata atctctatct cgtgctgcaa
     7921 atatttctgg agtcattggc attatttcac cataaagcac cgctaaaact tctacgatag
     7981 acagacttcc tccgtaatgt ccgaatccaa gatgattcaa tgttctaaga gtatttaatc
     8041 ggatgttagt cgcaaatttt cttaacccat cttctctatt tttacttaaa atcatccctt
     8101 attcctccgt tgcagatggc tttttaataa aggatactcc aaacataact gctagaataa
     8161 gaacaagacc aatcacaatg cctgcttgtg agccaaattg atttaacatt cctaaaataa
     8221 ttcctgatag accaaaatct gcatctgaga aagttgatcc ttggaaacca agtcctccca
     8281 aaactggcat taaaaagact ggaagaaaac tgattaaaat accttgtaaa aatgctccaa
     8341 tagtggctcc acgaacacca ccagatgcat tcccaatgac acctgcagtc gctccacaga
     8401 agaaatgagg cacaacacct ggtaagataa caaccgttcc tgaagcaatc ataattacca
     8461 tacttactaa accaccaaca aaactagaga taaatccaat tagaactgca ttgggtgcat
     8521 aagtataaac aatcggacaa tccaaagcag gttttgaatt aggtacaaga cgctctgaaa
     8581 tacctttaaa ggctggaaca atttcgccca aaataaggcg aacacctgct aaaataacaa
     8641 atacccctgc tgcaaattga cctgctaatt gtaaagcata aactagacca cttgtaccac
     8701 tactgatttc tttttctata tattctgacc ctgcaaagat agctacaata atgtaaataa
     8761 ctgccatgga taaagtaata ctaacagtac tatcacgtaa aaaagctaaa ctctttggaa
     8821 atttaatgtc ctctgttgat tttgatttgt caccgataag gctaccagta aaaccactca
     8881 accaatatcc caaagaactg aaatgaccta aagctacctt gtcatttcca gttaattgaa
     8941 ccatatattt ttgcacaaat gctggggaaa tactcataat aataccgagt gctaatcctc
     9001 ctagtaagat gagaggcaag ctagtaaagc cagcaactga taaaatgacc gcaatcatac
     9061 atgccatata tagagtgtgg tgccctgtta aaaaaatata tttaaatcga gtaaaacgag
     9121 cgattaagat attgaacacc atgcctgcaa acataatcat tgcagtagct gagccatatg
     9181 ttgttaaagc tacagctaca attgcttcat tattcggcac aacgccagat aaatgaaaag
     9241 catgctcaaa catggtacca aatggattta aagaattttg tacaattcct gcaccaccag
     9301 atacaactaa gaaaccaaca aaggtcttaa ttccaccttt aataatatca ggtaatttct
     9361 tcttctgaag aactaatcct aagattgcaa ttaaagctac taaaatagct ggtgtactaa
     9421 caatatccaa tatgaacttc atcatgacgc tagcctccta tataagtcct ttttcttcac
     9481 aaagtttagt aattaattct cgtagttcat ccatatcaat aatactattt aagatacgaa
     9541 catctccaag atgactagct gaatcagcta gatcacgacc aacaatccaa atatcagctg
     9601 catttggatc tgctccacct aaatcataat gttcaacttc tacatccgaa acattcaaat
     9661 cactcaatac agattcaata ttcatctgta ccataaaact tgaacctaat cctgaaccac
     9721 aagctgtacc aatttttaac attatctaat cctcctgttt aattatcatt ttaatgtcat
     9781 catagttttt tgatgatatt aaagtttgaa catgattttt atctcttaaa attgttgtta
     9841 aatgtgacaa agcctttaaa tgactctcat tatcaatggc tgcaatacaa atcaacaatc
     9901 ttacctcttg ttctggatta tccaataaat aaatcggttc ttccaaaact aacattgaca
     9961 ttcctatttc attcacacct tcatctggcc gagcgtgagg aattgctact cccttcccta
    10021 aattaataaa aggtccaaac tcttctactt tttgaatcat tgcctcaggg tagttctcag
    10081 ttatcttatc ttgatccaaa agcggtttag ctgctaaacg aatcgcctcc ttccatccta
    10141 atttttgcga actaacctga taggtttctt tggtaataag ttgttctagc actggtacaa
    10201 tttcctttct atcatttttt tggtaaagat aattctttaa cgccaatctt aattccaatt
    10261 cttgtgtaat aattccatat cttttgacaa tattcaggat ttgttcaatc tcaaaatctc
    10321 catactctaa attcggaaaa tcttttaaca ctagttctac tagttgtatt gcttgctctt
    10381 cagtcatcat aaccgaaact agataatttg gcttttctgt ctccaccttt atggtagaaa
    10441 aaaccatatc atagtcacta ctagctttca cctgtaaatc atcaatcttt gaggttccta
    10501 taaactcaat ttgaggaaat aatgctaata gattctcttt taacatcaat gaagaactaa
    10561 caccattagg acaaatgatt gctgctttat accatttttg aggcaaagta tctgctttct
    10621 ttaaataacc tccgaaatgg ataacaaaat atgctgtttc actatcaggt atgggattgt
    10681 caatagcgtc catcaagggc atcaaagaat ctttgactaa ttcaaataaa tcaggataat
    10741 gttctttaac atgcaataca tattcatttg aactaggtag gccgaacttt aatctatagt
    10801 aagccggtat aaggtggcgg cgaagatttt ctctcaatcc ttccctttgt ttaaaatgta
    10861 acaaagaaat atcttccatt ctacttataa tagcctctgt taattgatta aagtaaaccg
    10921 gagcaacatc tacttcacct tcaaagcaac ttgataataa aacggtgata tagcgataat
    10981 catcctcaga aaacaccgta tctataattc ccaaatcaac cactgtatcc aataaaatag
    11041 tggttatatc ttgaataaca ggagatacta atgtctctga aagacatact ctttcaacat
    11101 ccctttgata cctacacaga atgaatacta aaccgaaaag gtaaactttt aattgattaa
    11161 caataggtac tagctgtagc ttctcataat aatctttaac tacctgatca atcaaatcat
    11221 aagttaatga atacccccaa ctggataaaa cataatccaa accccaaatc cctatggagg
    11281 attccagcaa ctcactaacc atttgaaaag ctaagcggtg cttattccac tctgaaccgt
    11341 gtaaagtata accttttgct ctactgtacc ctagctccaa atcattatct aacataatct
    11401 ttcttaatga ttgaatatca gataaggttg tattcttact tactttcaaa aagtcttggt
    11461 aatgactatt cgatataaaa tctaatcggc aaaaagtgta aagatagatt aaagctaagc
    11521 gagtcgactt tggtaaaacc aattcatccg acttaataat atctgtcaaa gactgcttcg
    11581 tacgatttga taaactatag cgaccttgct ttttatccag cactatccct ttattagcta
    11641 gataaggcac taaataatct attccttctt tgacttcctt tataggtaag ctcaccttaa
    11701 cagataattc atataatgat agctcacaat gatccatcaa agtcatcaaa ataactagtg
    11761 ctctataatc aaacatttct cttcctttct aactacaatt atagtgtaaa aattataaga
    11821 ataaaagagt atttgagcac aatataatct caccttttaa aagaaattat tgggctgaaa
    11881 gtttttagtg attatttttc aactttctat tgactttata aattaaagtg atatcattta
    11941 gatatcaaat aacaggaggt cattcttatg actgaaaaac taatcaattc aaaaccaaat
    12001 ggtgtattcg cattgattct cattgagttg acaatcgtac ttggtatctt tatatttata
    12061 atgggggttg gttcggaaaa catttttgga attattatcg gacctttact aatcgtaatt
    12121 gcaggtctag ctcatgctgg tttaaaagtt gtcaaacctc aagaagctct ggttctgaca
    12181 ctctttggta actatacagg taccatcaaa gaacctggct tttactttgt caatcccttc
    12241 agcgtagcag tcaaccctgc aaaccacact cgacttggac aaagtggtga tgttagcaca
    12301 aaatctcctt ttttaggagc taaatcatca aatgacaatg atgtaaatct tgaaattggc
    12361 aagaaacaga tttccctcaa agtcatgacc ttgagcaatt ctcgtcaaaa aatcaatgat
    12421 tgcttaggaa accctgtaga aatcggtatc gcggtaactt ggagagttgt cgataccgct
    12481 aaggcagtct tcaacgttga taactacaaa gaatatcttt cattgcaatg tgatagcgcc
    12541 ctccgtaata ttgtccgcat ctatccttac gatgtgtctc ctaatgtgga tactacgggt
    12601 gatgggcaag cagatgaagg tagtctccgt ggctctagcg aaattgttgc taaccgtatt
    12661 cgtgaagaaa tccaaagtcg tgttgaggat gctggcttgg aaatccttga agcacgtatc
    12721 acttacctag cttatgctcc agaaattgct gccgttatgc ttcaacgcca acaagcatct
    12781 gccattattg atgcacggaa gatgattgta gatggtgctg taggaatggt tgaaatggca
    12841 ctagaacggc tcaatgaagg ggaattggta gaacttgacg aagaacgaaa agctgccatg
    12901 gtttcaaatc tccttgtcgt tctttgtggc aatcatgatg cacaaccaat tgtcaacaca
    12961 ggaagccttt actaaagatg gctgaagcta aaaaaaagca gatccccctt cgactctcaa
    13021 caaagttata cgctgcactc gcatcatggg cagaagacga ctttcgttca gtcaatgggc
    13081 aaatcgaata tctcctcaca gagtgtgtca agcaacggaa gaaagacgga aaatacgtat
    13141 cagaaaccat tgacgaacca tttgagattg atatttaaca ccttctcctg tctgccgtgt
    13201 cggataggag aattttttca tctttttttg tcaagtaact ttactttaca aaaaaaatgt
    13261 gttatcctag tatggttgat gaaaatcagt agattgaatc gaatataaat acctaaagga
    13321 gaaatcaaaa tggcagtacc tgcacgtcgc acttcaaaag cgaagaaaaa caaacgtcgt
    13381 acacactaca aagtaacagc tccatctgta aactttgacg aaactactgg agattactca
    13441 cgttctcacc gtgtatcact taaaggatac tacaaaggac gtaaaatcac taaagctgca
    13501 tcagctgaat aatagaaggg agataccatg cgcgtaaata ttacacttga acacaaagaa
    13561 tctggtgaac gcttgtacct tacttctaaa aacaaacgta acactccaga ccgccttcaa
    13621 ttgaagaaat actcaccaaa acttcgcaaa cacgttgtgt ttacagaggt taaataagca
    13681 ttcaatacga atcaatacag aagaaaaacg cttgatcagc gttttttctt tttgttaatt
    13741 tcactacatt gtaagggaaa tcattcgaat gactacattt ttcactacct tttttgaaat
    13801 aagaatacgg attcggacaa ttaaaagcaa gaggaactca aatgtaagtt ggggttcctt
    13861 ttttgtatat ttttcaaata acgcctctac actatttgta gcaaattcac caactacagt
    13921 tgtatcttag ttaaaataag ttagaatatg taagtgagta ccagatatac caagacatcg
    13981 tcaccatcta aggtatattc aaaatacaaa agttgaccaa ctagatttct gaatatcctt
    14041 atatatccat tcttaaaatt ggtttaaata gcgtagtctt ttaaactagt tttgagaatc
    14101 caaaaaatct tcctacatat gtaagaagat tttttagttc agaatgatta gatttagcta
    14161 atggatacct atcctaccca ctcaccgtta gcattgactc gatagccatc gggtgtagtc
    14221 gtgtttacta ataaatctcc tgaattatag gtatagtacc atttaccaga aactgtaacc
    14281 caaccagtag ccattgaacc tgattcattt aagta
//
LOCUS       DAVBCV010000052        14075 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBCV010000052 DAVBCV010000000
VERSION     DAVBCV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.052"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(394..2085)
                     /locus_tag="VQ516_001622"
     CDS             complement(394..2085)
                     /locus_tag="VQ516_001622"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2512032.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2221..2667
                     /gene="argR"
                     /locus_tag="VQ516_001623"
     CDS             2221..2667
                     /gene="argR"
                     /locus_tag="VQ516_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2512033.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2718..5252
                     /gene="mutS"
                     /locus_tag="VQ516_001624"
     CDS             2718..5252
                     /gene="mutS"
                     /locus_tag="VQ516_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2512034.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKLDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTENPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5399..7093
                     /gene="patA"
                     /locus_tag="VQ516_001625"
     CDS             5399..7093
                     /gene="patA"
                     /locus_tag="VQ516_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HET2512035.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDVPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(7423..7692)
                     /locus_tag="VQ516_001626"
                     /pseudo
     CDS             complement(7423..7692)
                     /locus_tag="VQ516_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7884..9650
                     /gene="patB"
                     /locus_tag="VQ516_001627"
     CDS             7884..9650
                     /gene="patB"
                     /locus_tag="VQ516_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2512036.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            9789..10478
                     /locus_tag="VQ516_001628"
     CDS             9789..10478
                     /locus_tag="VQ516_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2512037.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLDEL"
     gene            10482..10661
                     /locus_tag="VQ516_001629"
     CDS             10482..10661
                     /locus_tag="VQ516_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512038.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            10648..11997
                     /locus_tag="VQ516_001630"
     CDS             10648..11997
                     /locus_tag="VQ516_001630"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2512039.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            12121..13581
                     /locus_tag="VQ516_001631"
     CDS             12121..13581
                     /locus_tag="VQ516_001631"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2512040.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            13595..14029
                     /locus_tag="VQ516_001632"
     CDS             13595..14029
                     /locus_tag="VQ516_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2512041.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNTYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         4111 a         2714 c         3180 g         4070 t
ORIGIN      
        1 cttcggatta cgaatgaatt tgattgctgg cattatcaat catgaactag gattctagtt
       61 ttgcaggaag tctattattt ggttaggtga attagtgaag cgtttaggca agtgtctctg
      121 gttacgacgt catggactct aaatcgatta tatttagggg tcatgactag tgaagcagtt
      181 agctagttcg catataagcg gctagcgtct aacaattagg aactttagtt ccaataactt
      241 taagattacg acgttttagg acataaatcg atcatattta tgtcctaaaa ctagtgaagt
      301 atgtcctaaa actagtgaag cgcctagcca aagtccgaat aggatttggc gttagttact
      361 tagattgctt tgcaatcaag taactttggc gatttacatc ttctctggcg cttctactcc
      421 aagcaagcga agggcttctt tgagaacgac tgcggttgcg tagctgaggg ctagacggct
      481 gtcgcgttct gggctttcat ccaagatacg tgtatgtgca tagtatttgt taaaggattg
      541 agccaggcta attgcaaatt tagcaatgat agaaggttca aagttatctg ccgcacggtt
      601 gataatacgt gggaagtctt gaatgagttt aatgatttcc cagctttcag tatcattcaa
      661 gctatagttg ccagctgttt ctggtttgaa atcggctttg cgtaagatag attggatacg
      721 agcgtaggca tattgaacgt aaggtccagt ttcaccctcg aaggatacca tagcctctag
      781 gtcgaagtcg tatccatttg tacggtcggt tttgaggtca tagaatttaa tggctccaac
      841 cccaacagca tgtgctactt ggtctttgtt ttctagttca ggatttttag cctcgatttg
      901 gaccttggca cggctaacag cctctgcaac agtaggctct agcaagatga cattcccttt
      961 acgagtagag agtttcttcc cttcttttgt aaccaaacca aaaggaacgt gagtaatgtc
     1021 gtcactccag tcgtagccca tctcttgcaa gacagctttg agctgtttaa agtgggcaga
     1081 ttgttcttga ccaacgacat agatagattt agcaaattgg tattcgtttt tacggtagag
     1141 ggctgcagcc aagtcacgtg tgatatagag agttgcacca tcagacttct tgatgagggc
     1201 tggatgttca attccgtatt tctcaagatt cacaacttgg gcaccttctg attcaagaag
     1261 tagtcctttt tcagaaagaa tgtctacaac tgcatccatc ttatcattgt agaaggcttc
     1321 tccgttatag ctgtcaaatt caaccttcaa ttcattgtaa aggcggttaa attccactaa
     1381 actttcatcg cggaaccatt gccaaagagc gagagcttcc tcatctccat tttcaagttt
     1441 acggaaccat tcgcgcgctt cttcatccaa gctagggtca ttttcagctt cagcgttgat
     1501 gcggacatag agtttaagga gttcatcgat tggatgagct tttacagctt cttcgtcgcc
     1561 ccattttttg taggcaacaa tcaacatccc aaattgttta ccccagtctc ccaaatggtt
     1621 gaccttgacc gtttgataac cgattttttg gaaaatatgt gacaagctat ctccgataac
     1681 agttgaacgc aggtggccaa tagaaaatgg tttagcgata ttcggactag acatgtcgat
     1741 aacaacattt tcttgtttac caatattttg gtcagcatag tgttcttttt cagtggtaac
     1801 agcttgcaat acttgagcag aaatggcaga tttatcaagg aaaaagttaa cgtaaggtcc
     1861 tgttgcgaca actttttcaa aggcttggct gttcattttt tcagccagtt cagccgcaat
     1921 catttgtggt gctttacgtt cgactttggc aagagaaaaa gcagggaaag cgatgtcacc
     1981 catttctgag tttttagggg tttccagtaa ctttaaaata gcctcttggt ccaggctatc
     2041 aatgatgcta gccaattcgc tagcaatcaa ttcttttgta ttcattaaga gctccttttt
     2101 ggacttttct actattttat cacaatttta aagaaagaag aaaaaatttt tgaaatctcc
     2161 tgtttttttg gtataatatg gttataaata tagttataaa tatgcacgca agaggatttt
     2221 atgagaaaaa gagatcgtca tcagttaata aaaaaaatga ttactgagga gaaattaagt
     2281 acacaaaaag aaattcaaga tcggttggag gcgcacaatg tttgtgtgac gcagacaacc
     2341 ttgtctcgtg atttgcgcga aatcggcttg accaaggtca agaaaaatga tatggtgtat
     2401 tatgtactag taaatgagac agaaaagatt gatttggtgg aatttttgtc tcatcattta
     2461 gaaggtgttg caagagcaga gtttaccttg gtgcttcata ccaaattggg agaagcctct
     2521 gttttggcaa atattgtaga tgtaaataag gatgaatgga ttttaggaac agttgctggt
     2581 gccaatacct tattggttat ttgtcgagat cagcacgttg ccaaactcat ggaagatcgt
     2641 ttgctagatt tgatgaaaga taagtaaggt cttgggagtt gctctcaaga cttatttttg
     2701 aaaaggagag acagaaaatg gcgatagaaa agctatcacc cggcatgcaa cagtatgtgg
     2761 atattaaaaa gcaatatcca gatgcttttt tgctctttcg gatgggtgat ttttatgaat
     2821 tattttatga ggatgcggtc aatgctgcgc agattctgga aatttcctta acgagtcgca
     2881 acaagaatgc cgacaatccg atccctatgg cgggtgttcc ctatcattct gcccaacagt
     2941 atatcgatgt cttgattgag cagggttata aggtggctat cgcagagcag atggaagatc
     3001 ctaaacaagc agttggggtt gttaaacgag aggttgttca ggtcattacg ccagggacag
     3061 tggtcgatag cagtaagctg gacagtcaga ataatttttt ggttgccata gaccgcgaag
     3121 gcaatcaatt tggcctagct tatatggatt tggtgacggg tgacttttat gtgacaggtc
     3181 ttttggattt cacgctggtt tgtggggaaa tccgtaacct caaggctcga gaagtggtgt
     3241 tgggttatga cttgtctgag gaagaagaac aaatcctcag ctgccagatg aatctggtac
     3301 tctcttatga aaaagaaagc tttgaagacc ttcatttatt ggatttgcga ttggcaacgg
     3361 tggagcaaac ggcatctagt aagctgctcc agtatgttca tcggactcag atgagggaat
     3421 tgaaccacct caaacctgtt atccgctacg aaattaagga tttcttgcag atggattatg
     3481 cgaccaaggc tagtctggat ttggttgaga atgctcgctc aggtaagaaa caaggcagtc
     3541 ttttctggct tttggatgaa accaaaacgg ctatggggat gcgtctcttg cgttcttgga
     3601 ttcatcgccc cttgattgat aaggaacgaa tcgtccaacg tcaagaagta gtgcaggtct
     3661 ttctcgacca tttctttgag cgtagtgact tgacagacag tctcaagggt gtttatgaca
     3721 ttgagcgctt ggctagtcgt gtttcttttg gcaaaaccaa tccaaaggat ctcttgcagt
     3781 tggcgactac cttgtctagt gtgccacgga ttcgtgcgat tttagaaggg atggagcaac
     3841 ctgctctagc ctatctcatc gcacaactgg atgcaatccc tgagttggag agtttgatta
     3901 gcgcagcgat tgctcctgaa gctcctcatg tgattacaga tgggggaatt atccggactg
     3961 gatttgatga gactttagac aagtatcgtt gcgttctcag agaagggact agctggattg
     4021 ctgagattga ggctaaggag cgagaaaact ctggtatcag cacgctcaag attgactaca
     4081 ataaaaagga tggctactat tttcatgtga ccaattcgca actgggaaat gtgccagccc
     4141 actttttccg caaggcgacg ctgaaaaact cagaacgctt tggaaccgaa gaattagccc
     4201 gtatcgaggg agatatgctt gaggcgcgtg agaagtcagc caacctcgaa tacgaaatat
     4261 ttatgcgcat tcgtgaagag gtcggcaagt acatccagcg tttacaagct ttagcccaag
     4321 gaattgcgac ggttgatgtc ttacagagtc tggcggttgt ggctgaaacc cagcatttga
     4381 ttcgacctga gtttggtgac gattcacaaa ttgatatccg gaaagggcgc catgctgtcg
     4441 ttgaaaaggt tatgggggct cagacctata ttccaaatac gattcagatg gcagaagata
     4501 ccagtattca actgattaca gggccaaaca tgagtgggaa gtctacctat atgcgtcagt
     4561 tagccatgac ggcggttatg gcccagctgg gttcctatgt tcctgctgaa agcgcccatt
     4621 taccgatttt tgatgcgatt tttacccgta tcggagcagc agatgacttg gtttcgggtc
     4681 agtcaacctt tatggtggag atgatggagg ccaataatgc catttcgcat gcgaccaaga
     4741 actctctcat tctctttgat gaattgggac gtggaactgc aacttatgac gggatggctc
     4801 ttgctcagtc catcatcgaa tatatccatg agcacatcgg agctaagacc ctctttgcga
     4861 cccactacca tgagttgact agtctggagt ctagtttaca acacttggtc aatgtccacg
     4921 tggcaacttt ggagcaggat gggcaggtca ccttccttca caagattgaa ccgggaccag
     4981 ctgataaatc ctacggtatc catgttgcca agattgctgg cttgccagca gaccttttag
     5041 caagggcgga taagattttg actcagctag agaatcaagg aacagagaat cctcctccca
     5101 tgagacaaac tagtgctgtc actgaacaga tttcactctt tgatagggca gaagagcatc
     5161 ctatcctagc agaattagct aaactggatg tgtataatat gacacctatg caggttatga
     5221 atgtcttagt agagttaaaa cagaaactat aaaaccaaga ctcactagtt aatctagctg
     5281 tagcaaggag acttctttga caattctcca cttttttgct agaatgacat cacacaaaca
     5341 gaatgaaaag gagctgacgc attgtcgctc ccttttgtct attttttaag gagaaagtat
     5401 gctgattcag aaaataaaaa cctacaagtg gcaggccctg gcttcgctcc tgatgacagg
     5461 cttgatggtt gctagttcac ttctgcaacc gcgttatctg caggaagtct taggcgccct
     5521 ccttactggg aaatatgaag ctatttatag tatcggggct tggttgattg gtgtggccgt
     5581 agtcggtcta gttgctggtg gactcaatgt tgtcctcgca gcctatattg cccaaggagt
     5641 ttcatccgac cttcgggagg atgccttccg taaaattcaa accttttctt atgctgatat
     5701 tgaacaattt aatgcgggaa atctagtcgt tcgaatgaca aatgatatca accagattca
     5761 gaacgttgtc atgatgacct tccagattct tttcagactt cccctcttgt tcatcggttc
     5821 gtttatccta gcggttcaaa ccttaccttc tctgtggtgg gtgattgttc tcatggtagt
     5881 cttgattttt ggtttgactg ctgtcatgat gggaatgatg gggcctcgtt ttgccaagtt
     5941 tcaaaccctt cttgagcgca tcaatgccat tgccaaggaa aatttacgtg gcgttcgtgt
     6001 ggtcaagtcc tttgtccaag aaaaagagca atttgctaag tttacagagg tctcagacga
     6061 gcttcttggt caaaaccttt acattggtta tgccttttca gtagtggaac cctttatgat
     6121 gttggttggt tacggggcgg tcttcctctc tatttggctg gtcgcgggaa tggttcagtc
     6181 ggatccgtct gttgttggtt ccatcgcttc ttttgttaat tacctaagcc agattatctt
     6241 taccattgtt atggttggat ttttgggaaa ttctgtcagc cgtgccatga tttccatgcg
     6301 tcgtattcga gaaattcttg acgcagagcc agctatgacc ttcaaggatg tcccagatga
     6361 agagttggtt ggaagcctta gctttgaaaa tgtgaccttt acctatccaa tggacaagga
     6421 accgatgctg aaagatgtga gctttactat tgaacctggt caaatggttg gtgtagttgg
     6481 agcgactggt gcaggaaagt caaccttggc tcaattgatt ccacgtctct ttgatccaca
     6541 ggaaggggct atcaaaatcg gaggtaagga tattcgagaa gtgagtgaag gaaccctgcg
     6601 taaaacagtt tccatcgttc tccaacgtgc cattcttttt agtggaacga ttgcagataa
     6661 cttgagacag gggaagggaa atgctactct atttgaaatg gagcgcgcag ccaatattgc
     6721 ccaggctagt gaattcattc atcgtatgga gaaaaccttt gaaagtccag ttgaagaacg
     6781 gggaaccaat ttctctggtg gacaaaaaca aaggatgtcg attgcgcgtg ggattgtcag
     6841 caatccacgt attctgattt ttgatgattc gacctcagcc ttggatgcca aatcagagcg
     6901 cttggtgcaa gaagctttga ataaggactt gaaggggacg acaaccatta ttattgctca
     6961 aaaaattagc tcggttgtcc atgcagacaa gatcttggtt ctaaatcaag gacgattgat
     7021 tggtcaaggc acgcatgcag acttggttgc caacaatgcc gtttaccgtg aaatctatga
     7081 aacacagaaa tgaaagacaa actataagaa aagtcaatag ttttatctaa actatttctt
     7141 atttcaattt gatgatttgg cgatgatttt agagcacggc aaaaagccct tgaaaaagtc
     7201 cattttttca aaggtaatcc tgtgttgatt tcagaaatta catcactttt tgttcgtcaa
     7261 atggcagctc ttttttagga tataaaacag ggttcggata agtttttttg caaggtggat
     7321 gatggctaca ttgtaatgtt ttccttgtta taacttagtc ttaagatagg ccttaaaagc
     7381 aggtgaaaag tgagggcatg ctttggcagc ttgtatgagt acctaccgca gatgagggga
     7441 accccgtttg accattcttc cagctaaatc aatctgacct gactgataaa tagaagaatc
     7501 cagtccagcg aaagcttgta attgagcagg attatcaaag gcatgaatat ttcgaatctc
     7561 ggctaaaatg accgccccta aacgattccc aatcccagta accgtcgtga tgaccgagtt
     7621 gaactcagcc atcaagtcat tgacacattt ttccgccttg tcaatgagcc tcttgtaatg
     7681 tttgatgatt tcattacacg agataaaacg tctatgcgtt atcaaactca ttaccaatta
     7741 aaacaaaaag ctgtggttag atcctttcgg aaatcgtcaa gcgattggag gaaatgaact
     7801 aatccacagc ggcttattcc aagtatacca cttgggcttt ggcagtagct aactgcgcta
     7861 aatataatat aaggaggagt aaaatgaaga cagttcaatt tttttggcat tattttaagg
     7921 tctacaagtt ctcatttgta gttgtcatcc tgatgattgt tctggcgact tttgcccaag
     7981 ccctctttcc agtcttttct ggacaagcgg tgacgcagct agccaattta gttcaagctt
     8041 atcaaaatgg caatccagaa cttgtatggc aaagcctatc aggaatcatg gtcaatcttg
     8101 gcctgctggt tttggttcta tttatctcta gtgtaatata catgtgtctc atgacgcacg
     8161 tgattgcaga atcgaccaac gagatgcgca aaggcctctt tggtaagctt gctcagttga
     8221 cggtttcttt ctttgaccgt cgacaagatg gcgatatctt gtctcatttt accagtgatt
     8281 tggataatat cctccaagcc tttaacgaaa gcttgattca ggtcatgagc aatattgttt
     8341 tatacattgg tctgattctt gtcatgtttt cgagaaatgt gacgctggct ctcatcacca
     8401 ttgccagcac cccattggct ttccttatgc tgattttcat cgtgaaaatg gcacgcaaat
     8461 acaccaacct ccagcagaaa gaggtaggga agctcaacgc ctatatggat gagagcatct
     8521 caggccaaaa agccgtgatt gtgcaaggaa ttcaagagga tatgatggca ggatttcttg
     8581 aacaaaatga gcgcgtgcgc aaggcaacct ttaaaggaag aatgttctca ggaattcttt
     8641 tccctgtcat gaatgggatg agcctgatta atacagccat cgtcatcttt gctggttcgg
     8701 ctgtactttt gaatgataag tctattgaaa caagtacagc cctaggtttg attgttatgt
     8761 ttgcacaatt ttcacagcag tactaccagc ctattatcca agttgcagcg agttggggaa
     8821 gccttcagtt ggcctttact ggagctgaac gaattcagga aatgtttgat gcagaggagg
     8881 aaatccgacc tgaaaaggct ccaaccttca ctaagttgca agaaagtgtt gaaatcagtc
     8941 atatcgattt ttcatacttg cctgataaac ctattttgaa agatgtcagc atttctgccc
     9001 ctaaaggcca gatgacagca gttgttgggc cgacaggttc aggaaaaacg actattatga
     9061 acctcatcaa tcgcttttat gatgttgatg ctggtggtat ttattttgat ggtaaagaca
     9121 ttcgtggcta tgacttagat agtcttagaa gcaaggtggg aattgtattg caagattcgg
     9181 tcttgtttag cggaacgatt cgagacaata tccgatttgg tgtgccagat gctagtcagg
     9241 aaatggttga ggtagcagca aaagcaaccc acattcacga ctatatcgaa agtttgcctg
     9301 ataagtacga tactcttatt gatgatgacc agagcatctt ttcaacaggg cagaagcaat
     9361 tgatttcaat cgctcgaacc ctgatgacag atccagaagt tctcattctc gatgaagcaa
     9421 cttcaaacgt agatacggtg acagaaagca agattcagca tgccatggag gtggttgtag
     9481 caggtagaac tagtttcgtc attgcccacc gcttgaaaac cattctcaat gcagatcaga
     9541 ttattgtcct taaagatgga gaagtcattg aacgtggtaa ccaccatgaa cttttgaagc
     9601 taggtggctt ttattcagaa ctctatcaca atcaatttgt tttcgaataa gaaagaagtt
     9661 gtcctatgtg ggcagctttt tcttgtccat aaaaaatgtt tatcacagcc ttaaaaaaaa
     9721 catattagac gaaagtcatt ttgagtgata tgataggact atcgttagca ttcgaaagga
     9781 gaggcatcat ggctagaacg gttgtaggag ttgctgcaaa tctatgtccc gtagacgcag
     9841 aaggcaaaat cattcattca tctgtatctt gtagattcgc agagatcatt cgtcaagtcg
     9901 gtggtctccc tttagtcatt cctgttggtg atgagtcagt tgtacgtgat tatgtggaaa
     9961 tgattgacaa actcattttg acaggaggcc aaaatgttca tcctcagttt tatggagaga
    10021 aaaagaccgt cgagagcgat gattacaatc tggtccgtga cgaatttgaa ttggcactct
    10081 tgaaggaagc gcttcgtcag aataaaccaa ttatggcaat ctgtcgcggt gtccaacttg
    10141 tcaatgttgc ctttggtgga accctcaatc aagaaatcga aggtcactgg caaggactac
    10201 ctttcgggac atctcactct attgagacag tagaaggaag cgtggtggct aagctatttg
    10261 ggaaagaaag tcaggtcaac tcagtccatc gtcaaagcat taaagatttg gcacctaatt
    10321 tccgtgtaac tgctattgat tcaagagacc aaactattga agcgattgag tctatcgacg
    10381 agcaccgcat tatcggttta cagtggcatc cagagtttct ggttaatgaa gaagatggca
    10441 atttagaatt atttgagtat ttattggatg aactgtaaca attggaacat ctggtcgttc
    10501 tttcttttat tttttcaaaa tttttagaat gtggtatttt acgcaaacgt ttgaattctg
    10561 ataaaaattg gagaaattcg acaaaaaaac ttgaaaaaaa cgaaggtaag cgttatgata
    10621 gaaaagaaga aatattggag gaagaacatg tcacatatta aatttgatta ttcaaaagtt
    10681 ttagacaaat ttgttgcacc acatgaagtg gaatacatgc aatcacaagt aacagcagca
    10741 gatgaattga tccgtaaagg aactggtgct ggtagcgact tcttgggatg gttggacctt
    10801 cctgaaaaat acgaccgcga agaatttgac cgcatcttga aagctgctga gcaaatcaag
    10861 tcagacagcg atgttttggt tgtgatcggt attggtggat cttaccttgg tgcaaaagca
    10921 gcaatcgact tcttgaatca ccattttgct aacttgcaaa caaaagaaga acgcaaagct
    10981 ccacaaatcc tttacgcagg aaactcaatc tcatctactt accttgctga cttggtagag
    11041 tatgttgcag acaaagactt ctcagtaaac gtaatttcta aatcaggtac aacaactgaa
    11101 ccagcgattg ctttccgtgt ctttaaagaa ctcttggtta agaaatacgg tcaagaagaa
    11161 gctaacaaac gtatctatgc aacaactgac cgccaaaagg gtgctgttaa ggttgaagca
    11221 gacgctaacg gttggggaac atttgttgtt ccagatgata tcggtggacg cttctcagta
    11281 ttgacagccg ttggtttgct tccaatcgca gcatcaggag ctgacataaa agctcttatg
    11341 gaaggtgcga atgcagctcg caaagactac acttcagaca aaatctctga aaacgaagct
    11401 taccaatacg cagctgttcg taacatcctt tatcgtaaag gctatgcaac tgagatcttg
    11461 gtaaactatg agccatcact tcaatacttc tcagaatggt ggaaacaatt ggctggtgaa
    11521 tcagaaggaa aagaccaaaa aggtatctac ccaacttcag ccaacttctc aactgacttg
    11581 cactcacttg gtcaatttat ccaagaagga actcgtatca tgtttgaaac agttgtccgt
    11641 gttgacaaac ctcgtaaaaa cgtgcttatt cctactttgg aagaagacct tgacggactt
    11701 ggttaccttc aaggaaaaga cgttgacttt gtaaacaaaa aagcaactga cggtgttctt
    11761 cttgcccaca cagatggtga tgtaccaaac atgtatgtga ctcttccaga gcaagacgct
    11821 ttcactcttg gttacactat ctacttcttc gaattggcaa ttgccctttc aggttacttg
    11881 aatgctatca acccatttga ccaaccaggt gttgaagctt ataaacgtaa catgtttgcc
    11941 cttcttggaa aaccaggatt tgaagaattg agcaaagaac ttaacgcacg tctataatag
    12001 aagaaaagag tggtttgccc actcttttta ctctctttat ccatagaaat tggactcagc
    12061 caagacttgt gatataatat agaaagcaaa aaggcagacg cctagataat aggagaaact
    12121 atgtcaaaag atatccgcgt acgttacgca ccaagtccaa caggactact acacatagga
    12181 aatgctcgta cagcattgtt taattacttg tatgcgcgcc atcatggtgg aacatttctc
    12241 atccgtatcg aagatactga ccgtaaacgc catgtcgagg atggtgaacg ttcacaactt
    12301 gaaaaccttc gctggttagg catggattgg gatgaaagtc cagaatcaca tgagaattat
    12361 cgccagtctg agcgtttgga cttgtatcaa aaatacatcg accaactatt agctgaagga
    12421 aaagcctaca aatcttacgt tacagaagaa gagttggcag ctgaacgtga acgtcaagaa
    12481 gtagctggcg aaacaccacg ctacatcaat gaataccttg gtatgagtga agaagaaaaa
    12541 gcagcttaca tcgcagaacg tgaagcagca gggatcatcc caactgttcg tttggctgtc
    12601 aatgagtcag gtatctacaa gtggcatgat atggtcaaag gcgatatcga atttgaaggt
    12661 ggcaatatcg gtggtgactg ggttatccaa aagaaagacg gttacccaac ttacaacttt
    12721 gccgttgtta tcgatgacca cgatatgcaa atctctcatg ttatccgtgg agatgaccat
    12781 attgctaata caccaaaaca gcttatggtc tatgaagctc ttggttggga agctccagag
    12841 ttcggtcaca tgaccttgat tatcaactct gagactggta agaagttatc taaacgtgat
    12901 actaatacac ttcaatttat cgaagattac cgaaagaaag gttaccttcc agaagcagtc
    12961 tttaatttta ttgctcttct tggttggaac ccaggtggtg aagatgagat tttctctcgt
    13021 gaagaactca ttaaactttt tgatgaaaac cgcctcagca agtcaccagc agcctttgat
    13081 cagaaaaaac tagactggat gagcaatgat tatatcaaga atgcagacct tgaaaccatc
    13141 tttgaaatgg caaaaccatt cttagaggaa gcaggccgtt tgactgacaa ggctgaaaaa
    13201 ttagttgagc tctataaacc acaaatgaaa tcagtagatg agattatccc attgacagat
    13261 cttttcttct cagatttccc agaattgaca gaagcagagc gcgaagtcat gacgggtgaa
    13321 acagttccaa cagttcttga agcattcaaa gcaaaacttg aagcgatgac agatgataaa
    13381 tttgtgacag aaaatatctt cccacaaatt aaagcagttc aaaaagaaac aggtattaaa
    13441 gggaaaaatc ttttcatgcc tattcgtatc gcagtttcag gcgaaatgca tgggccagaa
    13501 ttaccagata caattttctt gcttggacgt gaaaaatcaa ttcagcatat cgaaaacatg
    13561 ctaaaagaaa tctctaaata aagaggatac aataatggac atctgggaaa agatgtacga
    13621 agaagcacag aaactataca atccacatga agtatcagac tttgtttatg ccaatcatgt
    13681 tgtagctgca gtagaagcag aagatggaca aatatttaca ggattctgta tggagggaac
    13741 ctgtggtgtt ttccatctct gcgcagaacg ggcggccctc ttcaatacgt accaattttc
    13801 aggacaaact aaggttaaga aagtattagc ctttcgagac aaaccacctt atggtggaag
    13861 ttcagccatg ccttgcggtg cttgtagaga attcctttta gagcttaatg ctgaaaataa
    13921 agatgcagaa ttcatgatgg actataatat aagaaaaaca gttaaagtcg cagaactaat
    13981 cccttattgg tggggagaag aacgtgcttc taagtttaat gagcgataga agagtcagta
    14041 taattctggc tttttttact ataagttgaa aaaat
//
LOCUS       DAVBCV010000053        14039 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBCV010000053 DAVBCV010000000
VERSION     DAVBCV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.053"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            98..1357
                     /locus_tag="VQ516_001633"
     CDS             98..1357
                     /locus_tag="VQ516_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2512042.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQIQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1433..1568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1607..2107
                     /locus_tag="VQ516_001634"
     CDS             1607..2107
                     /locus_tag="VQ516_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2512043.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2183..2551
                     /gene="rplL"
                     /locus_tag="VQ516_001635"
     CDS             2183..2551
                     /gene="rplL"
                     /locus_tag="VQ516_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2512044.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKTKLEEAGASVTLK"
     gene            2738..2908
                     /locus_tag="VQ516_001636"
     CDS             2738..2908
                     /locus_tag="VQ516_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512045.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2908..3156
                     /locus_tag="VQ516_001637"
                     /pseudo
     CDS             2908..3156
                     /locus_tag="VQ516_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3170..3510
                     /locus_tag="VQ516_001638"
                     /pseudo
     CDS             3170..3510
                     /locus_tag="VQ516_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3503..3883
                     /locus_tag="VQ516_001639"
     CDS             3503..3883
                     /locus_tag="VQ516_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2512046.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            3896..4039
                     /locus_tag="VQ516_001640"
     CDS             3896..4039
                     /locus_tag="VQ516_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512047.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            4132..4719
                     /locus_tag="VQ516_001641"
     CDS             4132..4719
                     /locus_tag="VQ516_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2512048.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            4921..5058
                     /locus_tag="VQ516_001642"
     CDS             4921..5058
                     /locus_tag="VQ516_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HET2512049.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            5237..7846
                     /locus_tag="VQ516_001643"
     CDS             5237..7846
                     /locus_tag="VQ516_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET2512050.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            7879..9819
                     /locus_tag="VQ516_001644"
     CDS             7879..9819
                     /locus_tag="VQ516_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET2512051.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            9816..11423
                     /locus_tag="VQ516_001645"
     CDS             9816..11423
                     /locus_tag="VQ516_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2512052.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            11438..12178
                     /locus_tag="VQ516_001646"
     CDS             11438..12178
                     /locus_tag="VQ516_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2512053.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            12181..13500
                     /locus_tag="VQ516_001647"
     CDS             12181..13500
                     /locus_tag="VQ516_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512054.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIASKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDVLIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
     gene            13634..13771
                     /locus_tag="VQ516_001648"
     CDS             13634..13771
                     /locus_tag="VQ516_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HET2512055.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT         4691 a         1960 c         2777 g         4611 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga
       61 agagagtaca acttgtcttt tagaaaagga gcctataatg aaagtctttc agcatgtaaa
      121 tatcgtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag cagtcaagga
      181 ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag ctgagcagat
      241 tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc
      301 aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta
      361 tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc
      421 cctgacagag atgctccagt caggaacaac aacctttaac gatatgtata atcccaatgg
      481 tgtggatatc cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc
      541 tccgactctc ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc
      601 catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca
      661 ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct
      721 aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa
      781 acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc
      841 cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca
      901 agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat
      961 tatccagatc caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa
     1021 taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag
     1081 tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc
     1141 taaggtcctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt
     1201 tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca
     1261 cctggtttat gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt
     1321 tgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg aaaaatttta
     1381 aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta tatacggttt
     1441 aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc gaggacaaga
     1501 cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg cgtttttctt
     1561 tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga gtgaagcaat
     1621 tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag ctgctgcatc
     1681 tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc ttcgtcgcga
     1741 gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc gtcgtgcagc
     1801 tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg cagtagcatt
     1861 ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta aaaacgctga
     1921 agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag aagagattct
     1981 tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt ctgtacttca
     2041 agcgccagtg cgcaacgttg ctcttgcagt caaagcggtt gcagaaagca aagaagacgc
     2101 ggcttaatct taagctacac agcgtagcct agctacgaaa aaaactatta taaaatttaa
     2161 aacttatttg gaggaaataa caatggcatt gaacattgaa aacattattg ctgaaattaa
     2221 agaagcttca atccttgaat tgaacgacct tgtaaaagct atcgaagaag aatttggtgt
     2281 aactgcagct gctcctgtag ctgttgctgc agctgatgca gctgatgctg gtgctgctaa
     2341 agattcattc gacgttgaat tgacatctgc aggcgacaaa aaagttggcg ttatcaaagt
     2401 tgtacgtgaa atcactggtc ttggtcttaa agaagctaaa gaacttgttg acggtgcacc
     2461 agcacttgtt aaagaaggcg ttgcaactgc agaagctgaa gaaatcaaaa ctaaattgga
     2521 agaagctgga gcttcagtta ctcttaaata atagagtatc gcaatttgat tgcggaatcc
     2581 ctatttacca gtctttgaga gcgataagcg attgatagaa actgataaat tgatttggtt
     2641 tcctgccaaa aattctttgg caggattttt gttttaataa actttcgggg gtagacacta
     2701 gattgtctac cccttttttt gtgtttcgga ggtaattatg caaaaggaag gacagtcatc
     2761 acgtcaattc gtgaagagtc atctcatgac tatcatggga gttgatattg taaaagcaac
     2821 tcagttagtt gatgaaatgg aacaagtagg tttaatccga tttgatgaat ttggaaatgt
     2881 tggaatttta gtcttggagg gacaataatg aagtgtatta ccgctaatca atatcagaca
     2941 tcagagcgtt attataagct ccccaaactt ttatttgaga gtgaacggta taagaatatg
     3001 aaactggagg ttaaggtggt ttactcagtt ttaaaagatc ggttggagtt gtctttgagc
     3061 aaaggttgga ttgatgagga tggggctatt tatttgattt attccaattc aaatttgatg
     3121 gcacttttag gctgttcaaa gtcaaaatta ctctccatgt gagtttgaag tgacattttt
     3181 agatgattac cataaaaaac ataactaccc actattttac gaatcctatc ttcaaaacgt
     3241 tatggaattc cttgaaagtc aagacataaa gaatggggtt gatgcctttg tagatgatca
     3301 tcaaaatctc gtttttgttt tatatggaca aggctatcga gccgagggaa aagagggaat
     3361 acttacaacc caagtaactg taaaagctta tgatgaagac aagaaaccga ttaacttcgc
     3421 aaatttatta gattccttaa tttattagat tccttaatcg tgtcagaata tcaaatggaa
     3481 ccgaatcttt gggaggtctc ccatgattga tctctatcta agtaaaaata gacaaagaaa
     3541 tcaacttctt ttagatttct tccaaaacta tggcatagag gtatcttgtc attcagtttc
     3601 tgaaatgaca aaggacaaat taattgagat gatgagctat tcttcagatt gttttgagtt
     3661 tttatctccc aatttattac gatttaagaa ccgtgacaat ctaagattaa cggatttcgt
     3721 tgagataatt ttaaaaaatc ctgaactaac catcagactt cctcttgcgg tttcaaataa
     3781 gcgagtttat ccaagtctga atttggaaga ggctagagct ctattgccaa gagatacgaa
     3841 acaattgatt tacatggcgc aaacacatta tttatctaac taaaggagaa gcaatatggc
     3901 tgaacgattt tgggagaact tgtccattat tttggctgaa agaaatatca gctggattga
     3961 gttaaccaga aaaatgtttg cgggagagtt tcactatcca agtgaattga atcgtttgta
     4021 tcaaaagatt cgccactaga agatggaaca atgaatgcct caaagtccat gggtagaaag
     4081 gattgtgcag gtattagatt tagattatga agatttattt cggaggtaac tatgaaaaga
     4141 ataattccag tttatatatt ccaacaagta aatgtcctat tggtatctct atacttattg
     4201 aaatttcttt gtatcggtga gttaactata ctacagattc tctatggttc gtcactcatt
     4261 tcttttttat ggatgtatgg ccaacgcaaa caagcgcata aggtcaatat gaaatctagg
     4321 atgaaatggc ttggtgttga atttgttagc ctactgatta taagtctatg ttttagtctg
     4381 attcatgctc aaggaagcac gaatcaagca aacttaattg gccttcaaca tcaaattcct
     4441 tggttttcat ttttattgtt cttaatcaat gcgagtatgg ttgaagaatt tctgtatcga
     4501 gaaattttat ggaatttggt cagaaaatta gatatcagag ttgctttgac aagtgtttta
     4561 tttgccttag cacatcatac aggaaccatt ctagcttggt gtttgtatgt ttcacttggg
     4621 atgtttttag gtatggtgcg ctataaatct gacttatggg gcagtatggg actacatttg
     4681 gtgtggaacc tactagttta tagtttgctg cttttttaaa ataggtgacc tgatttttag
     4741 gtaacttcta aaactaactt ccagttttgg gagagagatg gaagttactt tgaaaagtta
     4801 cggaaacatt attttctatg tgacaacatt gtataattgc ttgcatttta ctggtggaga
     4861 gagtataatg agggtggata aagtacttaa tcccaaaaat ttaaatagga ggagctagct
     4921 atggcagaag aagtattaaa tcttcaatta gtatctgttc aagttgacga aacagatgaa
     4981 gttgatggaa tgcgatttag tacattcagt acaaatcgtt gcggtaactg gagtgctttt
     5041 agttgggaaa attgttaatt catttaaatt gtaaacttta gagattctct ggagttatgg
     5101 atgtcgttcc aatatgtgca cgttggaatg ttagtgctta tatggggaga atccaaagga
     5161 ttctcccttt ttatttaaat aagtctgtct agctatagat atattaagtt tgataaaatt
     5221 tcttggagat tttgttatgg attataattt caatttggag catcctttct ttttcactaa
     5281 taatgattat agtacagata ctagcataaa gtaccaagta agtctgcctt ttaattggca
     5341 cgaagtaatg aataatgatg agtgggttta tcaataccct attggaaaat ttgttgaaag
     5401 acaagggtgg aaaatacaca ttagttcaga gtataacagt tcacatgaat tgttacaaga
     5461 tgtagcaaaa atttgccatg aaatgcgaat tccatttaaa catcttagta cagaagacaa
     5521 atttatcatg cgaaacggga aattggttag tcgaggattt tctggtaaat ttattacatg
     5581 ttatccaaat caaaatgaat tagagtcggt tttgcaaagg ttagaaagtg ccttaaagca
     5641 atataatggg ccatatattt taagcgataa acgttgggat gaagcaccaa tatatctgag
     5701 gtatggtgtg tttaggccgt caagggatga tgaaaagaag gttgctattg atgaattaat
     5761 tgttggcgat gaagttgtta aagatgaacg tttaccagta tttaaaattc caaaaggtat
     5821 agtgccccct gactttttga ataaatggct tgacaagaaa gataaaaaac agggagactt
     5881 tccctttatt atagataatg caattagatt ttcaaacagt ggtgggatat ataatgctag
     5941 gctaaaagaa gatgggaaaa aaataatttt aaaagaggct agaccgtata caggtctagg
     6001 atttgatggt acttattctt cagagaaatt agcttcggag tgcaaagcac taaaaattct
     6061 aaatgaatgg agtgagacgc ctaaaatata ctggcacggt aagatctggg agcacacctt
     6121 cttaggaatt gaacatatga agggcgttcc actaaataga tgggttacca ataattttcc
     6181 tttatatgaa gttgttgata agaccaaaga ttatctattg agagtttcaa agatagtaga
     6241 aaaactcatc gatttaacga ataagtttca ttctgaaaat gtatatcatc aagatttaca
     6301 tttaggaaat atcttggtta aggatgaaga tgagattagt ataatagact gggaacaggc
     6361 tgtcttttca aatgatgaaa aagttgtaca taaagttgca gcccctggtt ttagagcatg
     6421 gcgagaaaca cttcccagtg agatagattg gtatggtatt agacaaatag ctcattattt
     6481 atacatgcca ctagtaacta catcggattt gacatacaac tatgtaagtc aaaccaggat
     6541 agaaggaaaa aaactatttg agtctttggg ttatacaaga gaacatatag attatgtaga
     6601 aagtttattg tcatatctag attctaaatg tccacagatt gagaatattt ccaggaaaaa
     6661 agttttaaaa cccatgcatg agattagaac tatcgagagt gagcaagata tacaagattt
     6721 tattataaaa ttattgagag ggtttacgct aacttatgga cagtggcgta aagaatttca
     6781 atcaagattc ttcccagttc attattatgg tctgaatttt aatcaaggaa ttgcgttttc
     6841 agatttagct atcctttgga gttatcaaca actagctaag aaagtaaaaa atttcaagtt
     6901 tgatgactat tatgagattc gaacacaggt tattaatgag gcagtaaata attttaagaa
     6961 atcgtccctt tctggtcttt ttgatggtaa aattggtaca atctggctca tttatgaatt
     7021 tggggagata gatcgggcag ttgaactttt tacaactcat tttatagaga tatttgagaa
     7081 ttcacaaaat aaaaatttat atagtgggca ggctggaatt ttactggttg gactgtattt
     7141 tcttagtaaa ggggaaattg ataataaact aggagaagaa atactaatta ggcttagaga
     7201 atatacttta aattatatag aaaatccaga aactttttgt aaagttggcg ctagtgatgt
     7261 tcagagtaat gatccttatg agaattttgg tggattacta tatggtcacg caggggtagc
     7321 ttggctcttc ggtgaagctt ataaattaac tggtgaaagt atctataaaa atggtctaga
     7381 attggctgta gataaagaat tagttgctta taaagtagat agtaataatt cattgcagta
     7441 cagccaaggt catagattac taccatatct agcaactgga agtgctggcc tgcttttact
     7501 tattaataga aataaagaaa tcttgtcaag caaatactta aaatatttaa cttctttaga
     7561 gagggcaact gatgtagttt tttgtgtatt accaggttta tttaacggat tttgtggtct
     7621 tgaagttgct aataatatat atagtgatat cgatgataac ttttcaggac aaaagaaatt
     7681 aattgaacaa ctttatagat atttgtgtgt aattgaagaa ggatttgtta ttgcgggaga
     7741 taatggttta aagataacaa cagatattgc ttcgggattt gcgggtgttg caattggatt
     7801 ggtatcaata atggataata aattgacgat tcttcctcaa atataatatt tagataccat
     7861 tattaagggg tcatacttgt gaatcagagt aatgaaatat ttttaaatac aatacaggaa
     7921 aaaacacact ataagaggtc tatctcttca tcatttcagg aattaaaaaa atggaattct
     7981 ttgatattta ggagagggcg atattggtac caatttgata attggagtat acccaatact
     8041 ggtgtaataa aggaatggta tgtcgaagat aatgactgga ttcctaaggt tagttcttcc
     8101 agtttagaca attcaaattc acttaaaatt gtctttgatg tggaaaaata tactttgcca
     8161 gatgatagta tcttacagga tgttaggatt tcatctgatg agagttatat agttttggtt
     8221 gtatcctcac tgcgtagcac taatttaatt ggaataaaaa aatatactat ggaagagtta
     8281 tttatcattg aagatatttc agtagaaagt tcgttctatt tgggtaaatt cggtgttatg
     8341 tatactcgct ccaaagagta cgggaggcca agtaagttat tctataaatc ttttgatagc
     8401 tttacagaag aggaactgtt tgaagaaaac gaatgctctt ttcgattaaa aatagtccac
     8461 attgatagca ataattgttt tgtaaagtca gtagactttc agaaaggcag gatttttcta
     8521 tacagtttcg accggacggg ttttgttaga cactcttaca cagaaacggt agccccaaca
     8581 cctagagata tcgctctatt tagtacagaa aaagcacagt actttcttgg gctctcttca
     8641 actgaagaaa agaaagatca actaattcta agggaaactt cttcgggaga cagagtttct
     8701 attactatac catatcaaga tagggctaga agagttcact gtataggacg ttatatcctt
     8761 ttagactgtt caaacgcagc gaattccgtc ttttatcttg ctacatttaa aaataatagt
     8821 ttgagggagt tgagtgttaa taagataaca tttgatgaaa agacaacact ttatgaaaat
     8881 tcattttctg atcgtgttct tctgttttta gagaggagaa ctttttacga aaaaatactt
     8941 agttttgatt taatagaaaa tacattaaaa actgaatttg aaagacccat cataaaatcg
     9001 aataatacta aatatttttc taaggtgatt tggactaaaa aagacagtta tgatgtaagc
     9061 ataccgattt ctttattttg gaaaagtgaa gatacggatg agctcccaag aagaaaaaaa
     9121 tgtattctaa gtgtctatgg ggcttatggt aagaatgata attctgattt agatgaaatt
     9181 atgttatcta taatagatgc aggttttatt tatgctatag ttcatgtaag aggtggaggc
     9241 tatttgggtg gtgaatggta tcgctcagga aaggcgttaa acaaatggaa ttctatcaga
     9301 gattttattg agggagtaaa ttatttaagg gaaaatgatg taattgacag taagcgatta
     9361 ggtttaataa cttctagtgc aggtggaata attgctggtg cggtgttgaa cgaggaaaaa
     9421 aatttattgc aaagcattct cttattttcg ccatttataa atccttatga tacacttcag
     9481 aatccaaatg atcctctttc taagaccgaa atagcagagt ggggagatat tagagaccct
     9541 gaagtaaagg cttatataaa gtcatattcg cctatgcaaa atattgaaaa ggcacgagac
     9601 tcaaatactg ttatagttaa tattttgggt gagaaagacc catatattaa taataacgaa
     9661 gtgatagagt ggtcaaagaa attaaattct attggagtta aaagtttgtt gtatttaaac
     9721 aaagcagctg gtcatggagg ttttactcca tcagatgttc tcttaatgat tgatacttta
     9781 aattatttct ttgaagaagt gggaaggaat aacttatgaa ttatttaaaa tttataaaaa
     9841 agactaaact tatattaatg gggattttaa tatttctatc ttcgtttaat ggtgtcttgc
     9901 tctcaggaat tattgtatat gcaggtagtt taaatcagac ttcctcattt tctgacgttc
     9961 taagatttgg tgctataagt attctgggtt ggtcagcaat ctatatatca aattactatt
    10021 tagaagtaac ggaagcatca ataacgaaag atataaatgt aaaaattaaa caaggatatt
    10081 ttagagaaca gtatctttct tctgaaatgg ttaaagatta ctcatctatt atttcagttt
    10141 tgtcaaatga tttgagatta atcgaagaaa actattttag acaaattttt gaaataattt
    10201 cttcaatatt gttgttcatc gtctctctaa gttttatgct ttatttaaac ttcctagttt
    10261 caataatatt tattgtatta tcagcattgc cgattatagt acctgtcttt atgaagaaga
    10321 tgttgtctaa ttcagctaat gagtactcta acagcaatgc agagtatact cacataataa
    10381 aagaaatttt taatggtttt aagacattaa aatcttattc tgttactaaa gaaataatta
    10441 gtttgtcgga taaaaagttg gataaactag aagattctac ttttaatttg aaacgatcag
    10501 aggttctttc aaaattggtt gcagtactaa tttcaggttt ttgctttcta gttcccttgg
    10561 ttgtaggatg ttattttgta atttatcata aaagcctttc ttttagtgaa ctgataggta
    10621 ttttcttagc aaacgataaa gttttagggc cgatacaatc aattgcctat tcattaaata
    10681 agataaatac aaccaaagat ttaaggaaac cgtttttaaa atacttaagt ggagagaaga
    10741 attttataga cgctgaacat gataataacg gactgtatac ttcatcaata gatgagatac
    10801 acatgaaaga tgttgtatat tctattacac cagaaaataa attaagtatt gacttctcat
    10861 ttaagtcacc atttagggta ttattaacag gaacttctgg tagtgggaaa acaacgattt
    10921 taaatttaat taatggttct ttaaagccac aaaaaggtta tgtaaatttg ttatcacatg
    10981 ggaaaaagag ttcagattca ataccaacag ttgatcagac accatatatt tttgacacta
    11041 ctattcgtga gaacgtaact ttatttcaaa atgaatattt ttcagatgat cagataattg
    11101 aggtgttaaa aaaggtaaat ctatatgaag aattagaaaa gatagatata ctaaattatc
    11161 aatgtggtga aaatggtagt aatttgtctg gaggtcaaaa acaaaaaata gctttagcta
    11221 gagctctgat tagaaataat aaagtgtact tatttgacga aatatcagct aatttagata
    11281 atgataattc aaattccata catgatattc tgttcaattt aggtatttca tttattgaag
    11341 tttcacatca ttatgactta aatgacaaga gatacactga tatatataaa ttggaaaatg
    11401 gaacactttt caaaattaag tgattggagg caagaatatg gataaagatt atatattaaa
    11461 agtgaaaggg ctgtatcatc aatttttact aggaaataat aaaacgttgc aagtgctgaa
    11521 aaatgtttct ctttctgctt cgagaggaga atttataagt attctaggaa ttagtggttc
    11581 tggaaagtca actttattaa aatgtatttc tagtttgctt gaaccaacaa gtggggaagt
    11641 aattttaaat ggaatcaacc cctataaaat cagaaatgca aaattgtcaa gtataagacg
    11701 taacgaagta tcttttatat ttcaagcata caatttaata ccttccctgc cggtaataga
    11761 aaatatagca cttcctttgc gattatcaca aaaaaaatta actattaaaa atgtagaaaa
    11821 cttactcaaa agaatgaagt ttaatgctgg cttaaacgat tttgttggaa ctctgtctgg
    11881 tggagaacaa cagaaggttg ctatagctag agcggttatt gctgatagtg atataatatt
    11941 tgctgatgag ccaactgggg ctttagacag cgtttctcgt gaagtaattt ttgaattatt
    12001 gagagagtta gtaggggcgg gtaagtgtgt aattatggta acgcacgata tagaattggc
    12061 ctcgaaaact gatcgtgcat taatattgaa agatggaaaa attttcaaag aacttcatag
    12121 acctagcggg gaagagttgt ataaaatctt agaggtacaa tcaactacgg aggaatagag
    12181 atgctcagat tagtttacta tcaattttta cataataaga aacaatggtt aggcgtttca
    12241 ccagtaattt ttgtttcgag tttagttatg ggattggctg ttaatggggt aattaatgtt
    12301 gagaataatt cgcaagtatt tgtcggactt ccagatccta aaccaatttt tatgtttccg
    12361 atagtattcg gaggagtaac attattcttt gtgctctcta acataataaa tatgttggtt
    12421 gaaattttta gagatgatta cgaacttctg gaggtgttgg gagcaagtct ccttcaacta
    12481 tcatttcttg tggggggaca gatttttatc atttcctcga ttattagttt tattgcatat
    12541 ctatgctcta tatttgttac gtcaaattat tattattttt tacaatactt tttcggggaa
    12601 aatatattac ctgatatcca atttcaaacg agtgctgttg gatgtatcat tacagtagtg
    12661 ttgatttcat ttttagcctt tctaagtggt tgcttttata cgttcaagaa gattagaaat
    12721 aggaagagtt ctaaaataag gcatgttctt tcaatagtaa aaagaatact gctattggct
    12781 ggttttagtg ttatctggtt gctttctttg caacaaattt tccaagatag cacaatttta
    12841 gcgaaggccc agataatatt caatattgta atcttagata ttgtgattat ttatcaatta
    12901 tctcctttta tacagtcatg ttttatcaaa ctactttcta taattatatt tagaaataat
    12961 tttatgttca ttgcttcgaa gtggaattta ctatatcgta agccttatat aaaatctatc
    13021 agtgctgcaa ttacaggagc tatattacta atttctagtt ttcaaatgat ttctcagaat
    13081 atactatctc aatttcaaga tgacagtgat ttagagctaa aagtggcatt tattgtctat
    13141 gtaggagcac caattttgat agtacttgca aatattattt caattgcatt tttgtcatcg
    13201 catcaagaaa ggattgagat tcagcaattt gaaattttag gaacttctaa ttaccagatg
    13261 gttaagatta aagttggtga ggctatattt ttgacatttg ttacttcatt aattgcattt
    13321 ttacttaaca ttaaaattat ggctttaata tactactctt tagaggatgt tttaatagat
    13381 gatatgaatc ttttggggtt aatacttcca aattttatag tgtctattat tttatttatt
    13441 ttaatattta ttactaaaag ttcctatttt atctttaaga atgcaaaaat tatttcttaa
    13501 aaatttgtaa gttttcgcta tataaaatag ttaaatctat tttgtatcat tgcttgcatt
    13561 ttactggtgg agagaatata atgagggtgg ataaagtact taatcccaaa aatttaaata
    13621 ggaggagcta gctatggcag aagaagtatt aaatcttcaa ttagtatctg ttcaagttga
    13681 cgaaacagat gaagttgatg gaatgcgatt tagtacattc agtacaaacc gttgcggtaa
    13741 ctggagtgct tttagttggg aaaattgtta attcatttaa attgtaaact ttagggattc
    13801 tctgaagtta tggatgtcgt tccaatatgt gtacgttgga atgttagtgc ttatatgggg
    13861 ggaacagaat cctctcttga ttgaagacaa gttagccatt aggctggttt gtctctttgt
    13921 caactgtagt gggttgatat aatagtatta gtgagtggga taaaagtttc atttagttta
    13981 ttcagtacaa atttaacggg tcaagattta ttgaactgca ccccaatagt tagacagaa
//
LOCUS       DAVBCV010000054        14037 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBCV010000054 DAVBCV010000000
VERSION     DAVBCV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.054"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(166..780)
                     /locus_tag="VQ516_001649"
     CDS             complement(166..780)
                     /locus_tag="VQ516_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2512056.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1596..1865)
                     /gene="rpsO"
                     /locus_tag="VQ516_001650"
     CDS             complement(1596..1865)
                     /gene="rpsO"
                     /locus_tag="VQ516_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2512057.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGSRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2091..2636)
                     /locus_tag="VQ516_001651"
     CDS             complement(2091..2636)
                     /locus_tag="VQ516_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2512058.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWAFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            2875..3075
                     /locus_tag="VQ516_001652"
     CDS             2875..3075
                     /locus_tag="VQ516_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512059.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            3506..3646
                     /locus_tag="VQ516_001653"
     CDS             3506..3646
                     /locus_tag="VQ516_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2512060.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            3653..3829
                     /locus_tag="VQ516_001654"
                     /pseudo
     CDS             3653..3829
                     /locus_tag="VQ516_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            3887..5830
                     /gene="thrS"
                     /locus_tag="VQ516_001655"
     CDS             3887..5830
                     /gene="thrS"
                     /locus_tag="VQ516_001655"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2512061.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHGDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5879..6853)
                     /locus_tag="VQ516_001656"
     CDS             complement(5879..6853)
                     /locus_tag="VQ516_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2512062.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6846..7523)
                     /locus_tag="VQ516_001657"
     CDS             complement(6846..7523)
                     /locus_tag="VQ516_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2512063.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7679..8752
                     /locus_tag="VQ516_001658"
     CDS             7679..8752
                     /locus_tag="VQ516_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2512064.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHKIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8843..9145)
                     /locus_tag="VQ516_001659"
     CDS             complement(8843..9145)
                     /locus_tag="VQ516_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512065.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9213..9650)
                     /locus_tag="VQ516_001660"
     CDS             complement(9213..9650)
                     /locus_tag="VQ516_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2512066.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9726..10568)
                     /locus_tag="VQ516_001661"
     CDS             complement(9726..10568)
                     /locus_tag="VQ516_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2512067.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10680..11330
                     /locus_tag="VQ516_001662"
     CDS             10680..11330
                     /locus_tag="VQ516_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2512068.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11540..12174
                     /locus_tag="VQ516_001663"
                     /pseudo
     CDS             11540..12174
                     /locus_tag="VQ516_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            12305..12742
                     /locus_tag="VQ516_001664"
     CDS             12305..12742
                     /locus_tag="VQ516_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512069.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(12837..13136)
                     /locus_tag="VQ516_001665"
     CDS             complement(12837..13136)
                     /locus_tag="VQ516_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512070.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(13544..13906)
                     /locus_tag="VQ516_001666"
     CDS             complement(13544..13906)
                     /locus_tag="VQ516_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512071.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
BASE COUNT         4306 a         3064 c         2449 g         4218 t
ORIGIN      
        1 ttcttatttc attttactat atttataaga atctcctaat caaatcaaaa gccactctac
       61 aatagagtaa ctttaagatt gtccatttat actcaatgaa aaagccctct gaaaatcaga
      121 gagctgattt gagctcggac taaaatccta gtgaaacaaa aaaatctaca cgatcagaaa
      181 agtctctatc gtgccatttt catacatgat gtatagtcca agtagaatga atactaaggg
      241 cacaatgatt cgctcgtatt tttcaattgt ctcgaatatt aacggaatag aagataacac
      301 ccgactaatc tcgcaaaaga taattatgcc gattacaaac acaagcaagg ccacgagggt
      361 ctgtgaccaa tctaacgaag caaaataagg tatatagata cctaaattat ctccgccaga
      421 cgcaattgtc agcaatgtaa ctgtccaaaa cagttgattt gccttgcttt gttctaatct
      481 ttcaataatt tcttcctctt cttcttccgc atcttctcca acaattgcaa agcgaatccc
      541 taaatagata gggattaaac caagcaatcc aaccatccat tcttcaggca cgaaattaac
      601 gacataagca gcaactaaac tcgcccctac aagtaagcct gtgcctagat attgccccgc
      661 ataaatatgc catttctgtt tattctgtga tagctgtgca aataaaataa ttaaaataat
      721 taaataatcg atactggtgg aaatataaac accaatagca gatatgattg tctgtcccat
      781 aaaaaaaaac tcctgtatta gtcagtaata aatcagcaga ttttaggaga gcacagacac
      841 attaatttag ctatcgctag tgggtaatat cgaacgtagg aaagcatttt actcctcctc
      901 aaaacgtagt tacaaagtaa tttctaactg gaattcagtg actacttcca tgtaaagtat
      961 aacatactat actttacttt gtaaagagtg ggctcttcta gggggcttgc atacaactac
     1021 tcggcttgaa aagccgagta actgcgcacc tttatggtgt aattagctga taccgtttga
     1081 ggtttttgta gtctccaaaa ttgttaccga taaaacctca ataaaaaagc cctctgaagt
     1141 cagagagctg atttgagtcc gggctaaaat cctagtgaaa aagatgaaac tccttgtgct
     1201 catcgaacac tgtgtcattt ccctattttc atacggattt ttgacgccct tagcatcatg
     1261 attcttgcta gataaatcgt ttatagacta ggcgacgagg cgaagatcgt acgaaacttt
     1321 cagtgaaaca aaaaaaatct ccccgaaggg agaaaagctg gtttatttta ccttacagtg
     1381 ctaggaaccg taaccaaaga ttatacttga gtatatcgag gtaaggtgac aacacaaaaa
     1441 agctacccaa tttagagtag cttcattatc aagatatgct caattgaaca cggcctaagc
     1501 acttggcaaa aaagataaaa tctcctagaa actgaagttt cttcgtaaat tttcctattt
     1561 ttactttgtg cttttgacag cctttgtatc ttgaattagc gacgaagtcc tagagagttg
     1621 atcaactcac ggtaacggtt aacgtcgttt ttacgcaagt atgcaagcaa gttacgacgg
     1681 ctaccgattt ttttcatcaa tccacggtaa gtagcgtggt cttttttgtg ttgtttgatg
     1741 tgttcattaa ggtggttgat ttcccaagta aggacagcaa cttgaacctc tactgaacct
     1801 gtatcacctt cgtgacgtgc atattgtgcg atgatttcat tttttttctc ttttgagatt
     1861 gccatgatgt ttctcctttt tatttggctt catccgagtg acaggttggc atgcctgtaa
     1921 ccaagaagaa gttatttgtc tttacgacaa tccctattct accaagattt aaattttctg
     1981 tcaaacatta atactctcat agttattatt tttcaagtaa agagatacgt tcttttatat
     2041 gatgaccttt ttcaagttca tctaccaatc caatagcata atctgcataa ctaatctggc
     2101 ttattccttt tttcattagt agtaaaaatt tctcctgcta gaatgtagtt tcctgttttt
     2161 tcaccatcgg gaataaaatc tacagcagga ctgacaaaag cccagttgag attatttttt
     2221 gtacgaagaa gatccaattc atccgcttga gcttttgcta gtgacttaaa ttcttcagga
     2281 aaatcaggcg tatctaaaag tcgtgtcgtt tttgtttcat ctatataaag gcttcctgat
     2341 ccccctacaa ctagaaaacg tgtctgtgtt ccagcaagaa gctgattaaa tagttcgatt
     2401 gatttgctgt ggagcggtag cgtatctggt gtataagcac caaacgctga aataacagca
     2461 tcaaatccag taagatcatc ttttgtcaac tcaaataaat cttttttaat aatagactca
     2521 gcttgacttt tgttttcaga acgaacaata gccgttactt catgtcctcg tttgactgct
     2581 tcttcaacaa ttgccttccc cgcttgtcca tttgctgcaa taactgctag tttcattttt
     2641 tatacccctc ttgttgtaat tattttagtt acaacaattg tgacactctt aataatcaat
     2701 gtcaatagtc ttgcttaatt attatcaaaa tatttctacc aagaaaacta accatgattc
     2761 tagtgaaaag aaaaatcttc tttgtcaaca aatttacttt cttgttttaa acatactata
     2821 ataatcatag caagagatct aagttgtctg tttttttaaa acgaggtgat tatcatgcgt
     2881 agattctatt cccatctccc ctactatctg gtcatattat tcttttattg gccactttat
     2941 gagttgttct tgctagttgt ttctgacccc cttacactca agggactcta tataaacaat
     3001 cttctcttct ttacacctat ggtaatcttg attgtatcgt tactctatag ctaccgtttc
     3061 cgtttctcac tttgatggtt agttggtaac ggactgctct tttactttac tatcataacc
     3121 tttggtgtgt ttatactaat ttacttgcta atctatgaaa cagttgctct ggtgggcatg
     3181 gattctggta ttagcatcaa gcatattcta caaaaaatga aaaacaaaaa actttcacaa
     3241 aatccttgaa aaatctcaca atcatgctat aataatccat agagacaagt cacttagtcc
     3301 ctttctacta gagagtgcgt ggttgctgga aacgcatagg aagtctaaac tgatactact
     3361 cttgagtttt ttatgaaaac ataaaacggt ggccacgtta gagccgatca gaggtgtccc
     3421 tctcttttga ggtacataaa tgaaggtgga accacgttgc gacgtccttt cgaggatgtc
     3481 gcattttttt attaggatac taattatgga gttgcaagaa ttagtggagc gcagttgggc
     3541 aatccgacaa gcttatcacg aactggaagt taagcatcat gatttcaagt ggacggtaga
     3601 agaagacctc ttggctttat ctaatgatat tggaaatttc caatgactgg tgatgacaaa
     3661 gcaaggacgc tactatgatg aaacacccta cacactggaa caaaaacttt cagaaaatat
     3721 ctggtgacta ttagaacttt ctcaacgttt ggatatagat attctgacgg aaatggaaaa
     3781 cttcctctct gataaagaaa agcaattgaa cgttagaact tggaagtagt ctgctgataa
     3841 aaaatcaatg cttagaaact atgaaataat aaaaaaggag aacatcatga ttaacattac
     3901 tttcccagat ggcgctattc gtgaattcga atctggcgta acaacttttg aaattgccca
     3961 atctatcagc aattccctag ctaaaaaagc cttggctggt aaattcaacg gcaaactcat
     4021 cgacactact cgcgctatca ctgaagatgg aagcatcgaa attgtgacac ctgatcacgg
     4081 agatgccctt ccaatcttgc gtcactcagc agctcacttg ttcgcccaag cagctcgtcg
     4141 tcttttccca gacattcact tgggagttgg tccagccatc gaagatggtt tctactacga
     4201 tactgacaac acagctggtc aaatctctaa cgaagacctt cctcgtatcg aagaagaaat
     4261 gcaaaaaatc gtcaaagaaa acttcccatc tattcgtgaa gaagtgacta aagacgaggc
     4321 acgtgaaatc ttcaaaaatg acccttacaa gttggaattg attgaagaac actcagaaga
     4381 cgaaggcggt ttgactatct atcgtcaggg tgaatatgta gacctctgcc gtggacctca
     4441 cgttccatca acaggtcgta tccaaatctt ccaccttctc catgtagctg gtgcgtactg
     4501 gcgtggaaac agcgacaacg ctatgatgca acgtatctac ggtacagctt ggtttgacaa
     4561 gaaagacttg aaaaactacc ttcaaatgcg tgaagaagct aaggaacgtg accaccgtaa
     4621 acttggtaaa gagcttgacc tctttatgat ttcacaagaa gtgggacaag gtttgccatt
     4681 ctggttgcca aatggtgcga ctatccgtcg tgaattggaa cgctacatcg taaacaaaga
     4741 gttggcttct ggctaccaac acgtctacac tccaccactt gcttctgttg agctttacaa
     4801 gacttctggt cactgggatc attaccaaga agacatgttc ccaaccatgg acatgggtga
     4861 cggggaagaa tttgtccttc gtccaatgaa ctgtccgcac cacatccaag ttttcaaaca
     4921 ccatgttcac tcttaccgtg aattgccaat ccgtatcgct gaaatcggta tgatgcaccg
     4981 ttacgaaaaa tctggtgccc tcactggcct tcaacgtgta cgtgaaatgt cactcaacga
     5041 cggtcaccta ttcgttactc cagaacaaat ccaagaagaa ttccaacgtg cccttcagtt
     5101 gattatcgat gtttatgaag acttcaactt gactgactac cgcttccgcc tctctcttcg
     5161 tgaccctcaa gatactcata agtactttga taacgatgag atgtgggaaa atgcccaaac
     5221 catgcttcgt gcagctcttg atgaaatggg cgtggactac tttgaagccg aaggtgaagc
     5281 agccttctac ggaccaaaat tggatatcca gattaaaact gcccttggaa aagaagaaac
     5341 cctttctact atccaacttg atttcttgtt gccagaacgc ttcgacctca aatacatcgg
     5401 agctgatggc gaagatcacc gtccagtcat gatccaccgt ggggttatct caactatgga
     5461 acgcttcaca gctatcttga ttgagaacta caagggggcc ttcccaacat ggctggcacc
     5521 acaccaagta accctcatcc cagtatctaa cgaaaaacac gtggactacg cttgggaagt
     5581 agccaagaaa ctccgtgacc gcggtgtccg tgcagacgta gatgagcgca atgaaaaaat
     5641 gcagttcaag atccgtgctt cacaaaccag caagattcct taccaattaa ttgttggaga
     5701 caaagaaatg gaagacgaaa cagtcaacgt tcgtcgctac ggccaaaaag aaacacaaac
     5761 tgtctcagtt gataattttg ttcaagctat cctagctgat atcgccaaca aatcacgcgt
     5821 tgagaaataa gagtctagca taaaagcctc caatctggag gctttttctc atctattttt
     5881 actcaaggac taagttcact tgagcaaact gaatccgcac tgtcgttcct tttccgacct
     5941 cagactcgat acgaatctgg tgccccagtt cttcagaaat tttcttagat agataaaggc
     6001 caagtccaga ggactgctgg gtcaaacggc cattgtatcc tgaaaagcca cgttcaaata
     6061 ctcggaggac atcactgttt tttatcccga ttcccgtatc tttgatacaa agctcttggt
     6121 catccatata aatctccaga ccaccttcct tggtgtactt gagactgttt gagatgattt
     6181 gctcaataac cactagcagc cactttttat ccgtcacgat ttctttatca aggtcatgta
     6241 gattgacatt taagcctttt tgaataaaga aaagagcata tttacgaatt atttccttga
     6301 ccaagtcctc aatttgaacc tgctttaaga gcaaatcatc atggaaactt tctaaacgca
     6361 ggtactgtaa aactaggttg gtataagagt cgattttgaa aatttcctgt tctagctgct
     6421 gcttcagttg gcggtcgacc acttctgcaa ctaagagttg actggctgca atgggggtct
     6481 ttatctgatg gacccacaag gtatagtaat ccagcaaatc cgtcagtttt ctttctgctt
     6541 ttgacctctg ctgatagagt tccatctcac gcgcttctaa tttttctgct aaagctattt
     6601 ccaaaggaga cttggcttcc ctctctccat agagaagttc ctggcgatag acctgcattt
     6661 ccaccaatat gtcccaagtg aaaaataaga tggttacaaa gcaacacaag aagaaaaagt
     6721 agaggaagta aattcctaga ctggcaaata aaaactgaaa gagtaagaca agaaatgcca
     6781 aagaaagcag atagataaaa agacgactac gggagcgcag ataggctaga aaaaattgtt
     6841 tccaatcaag catgcttcaa tccgtaccct attcctttct tggtctcgat aaatcctacc
     6901 aatccctgct cctccaactt tttacgcaaa cgagccacat tgacagagag ggtattatca
     6961 tcaatgaaaa agtcactgtt ccaaagttcc cgcatcaggt cgtcacgtgc tacgatgttg
     7021 cctgcatgct caaataacac gcgtaaaatc tggaattcat tcttggtcaa attcaagact
     7081 tgcccttgat aatgtaaatc catggatttg gtattgagga taacaccagc atattccagc
     7141 aaactctcat cacgcccaaa ctcataggaa cgacgcaaca agccctgaac cttagctaaa
     7201 agaacctgct ggtcaaaagg cttggtcaca aagtcatccg cccccatatt gattgccatg
     7261 acaatatcca tagcctggtc tctcgaagaa agaaacatga taggtacctt ggaaatcttg
     7321 cggatttcct gacaccagtg ataaccatta aacaagggca aaccaatatc catgaggacc
     7381 agatgaggtt ccgactgaac aaatagactc aaaacttcca taaagtcttc taccaggacc
     7441 acttcaaatc cccattcaga gagcattttc ccaatctgtt gacgaatgac ctgatcatct
     7501 tctattaata aaatcttgtg catgcgcttc tccttttcca ttattataac agatttttcc
     7561 atgctagatg gtctgaaact gaatttgaaa tagcctgttt ttagccagtg caaacaggct
     7621 atgctactag ctaatttgag ggaaatttgc taagataaat aaaaagaaag gagctcttat
     7681 ggccaatatt tttgactatc tgaaagatgt cgcatatgat tcttattacg accttccctt
     7741 gaatgagtta gacattctaa ccttaataga aatcacctac ctctcctttg ataatctggt
     7801 ctccacactt cctcaacgtc ttttagatct agcacctcag gttccaagag agcccaccat
     7861 gcttactagc aaaaatcgcc ttcaattatt agataaattg gctcaacaca agcgcttcaa
     7921 aaattgcaaa ctctcccatt ttatcaacga catcgaccct gaactgcaaa agcaatttgc
     7981 ggctatgact tatcgtgtca gcctcgatac ctatctgatt gtctttcgtg ggacagatga
     8041 cagtatcatt ggctggaagg aagatttcca cctgacctat atgaaggaaa ttcctgctca
     8101 aaagcacgcc cttcgctatt taaagaactt ttttgcccat catcctaagc aaaaggttat
     8161 tctagctggg cattccaagg gaggaaatct cgctatctat gctgctagcc aaattgagca
     8221 aagtttgcaa aatcagatca cagcagttta tacatttgat gcacctggtc tccatcaaga
     8281 attgacacag actgcgggtt atcaaaggat aatggataga agcaagatat tcattccaca
     8341 aggttccatt atcggtatga tgctggaaat tcctgctcac aaaatcatcg ttcagagtac
     8401 tgccctgggt ggcatcgccc agcacgatac ctttagttgg cagattgagg acaagcactt
     8461 cgtccaactg gataagacca acagtgatag ccagcaagta gacacaacct ttaaagaatg
     8521 ggtggccaca gtccctgacg aagaacttca gctctacttc gacctcttct ttggcactat
     8581 tcttgaggct ggtattagct ctatcaatga cttggcttcc ttaaaggcgc ttgaatacat
     8641 tcatcatctc tttgtccaag ctcaatccct cactccagaa gaaagagaaa ccttgggtcg
     8701 ccttacccag ttattgattg atactcgtta ccaggcatgg aaaaatagat aatactcttg
     8761 aaaattaaat gtatacaaaa caaaagacct agaatacata ctttcatgtg cattctaagt
     8821 ctttttaaat agaatctaat agtcaataaa aatcaaagag cattgagaga taatggggct
     8881 tggaacgtcc ctctcgcttc aacaaaatga ccccattata gattaaaaag atgccactta
     8941 gaaaaagcaa aaaaggaagt aagacaaagg caaatacata aaaagctaac tgaacattct
     9001 cgtatccatt tttataaaaa aggtaggata gataaaaata acttgaaatg agggataata
     9061 aaaataatac tggattccac aaacttctat tatccttcca aaatgacact ataaaggcta
     9121 atacaattcc tataacgaga tacatttctt actcctttaa tagctacatt ttatcataat
     9181 tatccaaaga aaaaagaggg catttatccc tcttaatcct tcatctgact ctctgcatcg
     9241 gccacgactt tttctagact ggtttgacca agttctgcct ccatagtcaa ctgaattctc
     9301 tccaattttt gatccaaaac atcatgaata tgagctccta cagggcaatt tggattcgga
     9361 ttgtcatgga aactgaagag ttgacctgtc ttaccaagac attcgaccgc ctgataaaca
     9421 tctaaaagac taatatcctt aaggtccttg acaatatctg ttccgcccgt tccacgcgct
     9481 actgaaatca gctctgcctt cttcaactgg gacaagatct ttctgataat gacaggattg
     9541 accccgacac tagcagccag aaaatcactg gtcaccttgc ttttcttccc ctcgagggca
     9601 atgattatca gcatatgagt cgcaatggta aatctacttg gaatttgcat cctcttctcc
     9661 tttttacgag gctaccctgc ctctactctt ctttttctat tattataccc tttttagttg
     9721 taatgtcaat cgttaccact tttcaaccag tcgtctaact cccgatcgca accctctttc
     9781 tgagccaatt ctctcaaaaa ttcctgatga tgagtatggt ggatcccatt gaccagactt
     9841 tcatagtaaa cctcaaaata gggaagtctc aggtctttag ccagctgcaa ttcagctgcc
     9901 acatcgtaat ctacccgtcg gaagtccata tctaccaggc ccttgtcatc aaactccaaa
     9961 atcatatact gggcccgcaa gtccttccgt agctgagcgt ccaaaaagaa aggttggcca
    10021 atcgaacccg gattgacaat caattgccca ccagtcccgt aacgaagcaa ctgctggtga
    10081 atgtgtccat aaacagcaat atcacaggga ggatgagtca ccaagcggtc aaattcctct
    10141 tgtttgccag tatgaatcaa ctctcgcccc cagttcttat caggcagatg atggctaatt
    10201 cccactgtca aatccccaaa ctgacgatga atttgaagag gttgattgtg gagcacttca
    10261 atttcttcta gggaaatttc ctctaaaaca tactggcact ggcgcaagag atagcgttga
    10321 ctagggcgag tactatccaa ttccttacgg acaccatgcc aaagactgtc ttcccagttt
    10381 cccaaaactc tagccgtaat cggtagttga tccaacaagt ccaaaatcct tctacgccct
    10441 gtccctggca tgagaatatc tcccaaaagc cagtattcat ccactcctag ctgccgagca
    10501 tctgccaaaa cagcctccaa ggcggtggta tttccatgaa tatctgaaag aagagctatt
    10561 ttcgtcatat ccatctcctc gttttttctc ttgcaataag tataacataa aaagtcacag
    10621 ctagagaaat ctagcttttt ttgatatact agataaagat attagacaag aggaaacgaa
    10681 tgaccccaaa caaagaagac tatctaaaat gtatttatga aattggcata gacctgcata
    10741 agattaccaa caaggaaatt gcggctcgca tgcaagtctc tccccctgcc gtaactgaaa
    10801 tgatcaaacg aatgaaaagt gaaaatctca tcctaaagga caaggaatgt ggctatctac
    10861 tgactgacct cggtctcaaa ctggtctctg agctctatcg taagcaccgc ttgattgaag
    10921 tttttctagt tcatcattta gactatacaa gtgaccagat tcacgaggaa gctgaggtct
    10981 tggaacacac tgtctctgac ctgttcgtgg aaagactaga taaactgcta ggtttcccta
    11041 aaacctgccc ccacggagga actattcctg ccaagggaga actcctcgtt gaaatcaata
    11101 acctcccact agctgatatc aaggaagctg gcgcctaccg cctgactcgg atgcacgata
    11161 gttttgacat tctccattat ctggacaagc actcacttca catcggtgac cagctccaag
    11221 ttaagcagtt tgatggcttc agcaatacct tcactatcct cagtaacgac gaggatttac
    11281 aagtgaatat ggacattgca aaacaactct atgtcgagaa aatcaactaa tttctcaagt
    11341 cccctaccaa ccctgaaagt tttattttca gggttttatt tctatcattt tattttactt
    11401 tcatttggtg gtagaatatt tgaaaaaaaa tgggagaata tttgaaaaaa agaaaggtag
    11461 tttaacctat gaaaaaatca ctaaaaattt ttgctacatc taaatagttt gacctcttcg
    11521 gggttgcttt ggtcgttgga ttgcgattgc atctggttac ctcaactcac gcctcgataa
    11581 atttgtagac tggggaccat ggacagctct tgtacccttt ggattgattt ccgtaaccaa
    11641 cgttgggatt tccatgttgt ccactcgttt cacggggaaa ttaagcaaat gggaaaatta
    11701 ctttggtatt gttaatacca ttttgtccgg tgctattgat tatatccttg gaaataaggc
    11761 ggccatcatt acctatcccg tcaccttcct catttatacc tttgctataa agaaatggaa
    11821 agcttcgcaa gaaggcagac ccaaccaaat gagccaaaaa caggtaaaat tggcggccat
    11881 catcatttcc atcatcgcct tcctctttgc ctttgtgacc aactatatcg gatatggggg
    11941 caagatgaat ctccttgcct acgtaacaac tattgccttt gcactgtccc tcattgccaa
    12001 tgctttgaat gcattggcaa tgagggacag tggggctttt ggttgattta caatttcgtt
    12061 cagctgacaa aagctggtat tcaaggaaac ttcgccaata tcggtaaata catcttttat
    12121 atcctcaatg caatcggagc tttatttgtc tggaatgatg aagaagttag ataatagaag
    12181 gaaactcaaa taaagaatac tagccaactt aagagaatct cccctctgct atttttatca
    12241 ttttagttgt aactcccatt cttttagagt agaatgaagt tagataaaag agggaggtct
    12301 tgtcatgaaa aaactctata gaatccattt tatagcaatt gcagttattg atttgctact
    12361 atttgcattt tttatcacta gactcgagac atcttttgag tggctcttgc tctctggttt
    12421 aattttcttc cttgctcaag gactcctgct atttctcttg gtcgtccgac tcaaacatca
    12481 attcgctgag atttatcctc aaatcaataa aaagattcgc ttctactatt taggggttct
    12541 caccattgat tttctatttt ttgttctctt agccttcatt agttctcagc gtttttcatc
    12601 tcttatgcca atcatcactg cttgccattc tactttttat tatatgacag ctgactacct
    12661 aagagaaaac tatccagact tttacgacaa acacatctct aacacgtata ctattttatc
    12721 gatttcttct tttttagtat aagacagctc gttctccaga aatattcatt aactttcatt
    12781 tttatacttt tcctttcgat tttcaaaaac aaggggaggg attaacctcc cttatattag
    12841 acataacttt taggatcaca attgtatgtt cctttcgaaa tatccccaaa aatcgtagcc
    12901 cctgttaaat aatgatttct tgtagaagca tatgctattt tcttcatcac atccagtctc
    12961 gctactttta gctcgagatt aggattggaa aacatagcat ttttaatatc ttccaaatct
    13021 tgagaactag gtgcgagtag agttacatcg ccctccaatc tattagcctt gtctatataa
    13081 agactaactc tttcagcact ttctgttcgc agaaaattta gtacatttct attcattttt
    13141 ttacctcgta tttttctaat tattgatttt ttctagaaac aaaatcaatt caacggtgaa
    13201 gctctagctt tcatcttcat tcgtacctcc ttttgtgtat atctagcttt acataaacct
    13261 atgatttaga gtatagtttc tcaattattc tcagaaacta tggagatgat ttaattatca
    13321 tacaaatcaa agacctctcc ttgctcatct tcaccgtaac tctcgagttc ctcataaaag
    13381 tattccaaag cagtttgttc ttctggtgtc ataaagaaac tcctttctaa acaatattag
    13441 taaaaaatac gttcttattt ccatcctaaa taaagtagtg tttttatccc gtaataggtt
    13501 ttatttttgc ggagcattac actccgcatt aatattgata gtattaagaa tcattattac
    13561 ttaataaaaa ttgcgctctc aaacatagac gtatacaaaa tgccctatgt atacgtttcg
    13621 cttggaagac atctaagaaa aaattttgta gttcagacag taagattaat acatctttct
    13681 ctaacataag tgatgtctgg tactcccaat tattgctctg ttttaacgat gaaaaaggta
    13741 tagctgtctc ttttaatttt atccaatcta tacgttcttt tttaggaacg tattctctaa
    13801 cagcttgact aacaatactg gaatagctta cttcatagcc taatttattg taaacatcat
    13861 ctttaagctt atctaatatc tgtctggatt cagacgctaa acgcattcta acattctctt
    13921 ttcccatatt atcacccctt tctgacggga ttctgttatc ccgtaaatta agtatataac
    13981 aatttagaaa aaaaatcaag aattaagttg aaaaaaattc cacaatctaa aaattta
//
LOCUS       DAVBCV010000055        13682 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBCV010000055 DAVBCV010000000
VERSION     DAVBCV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.055"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..1537)
                     /locus_tag="VQ516_001667"
     CDS             complement(<1..1537)
                     /locus_tag="VQ516_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HET2512072.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYAASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGTGLDSVYHVPIYYKLTVTNDGSKLTFTYTVTYVDPTTRALGNLSKMGHG
                     YSIYNTGTSIQTMLTLGSDLGTPSDVKNYITDKDGVLVRSYNTSTMTTWGSGYTWGNG
                     AQMNGFFAKQGYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFKGRGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASESASTSASASASTSASASASTSASASASTSA
                     SASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASA
                     SAST"
     gene            complement(1535..1648)
                     /locus_tag="VQ516_001668"
                     /pseudo
     CDS             complement(1535..1648)
                     /locus_tag="VQ516_001668"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
     gene            complement(1887..2348)
                     /locus_tag="VQ516_001669"
     CDS             complement(1887..2348)
                     /locus_tag="VQ516_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512073.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(2315..2458)
                     /locus_tag="VQ516_001670"
     CDS             complement(2315..2458)
                     /locus_tag="VQ516_001670"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2512074.1"
                     /translation="MAYSTDFKQGALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KGT"
     gene            2727..3107
                     /locus_tag="VQ516_001671"
     CDS             2727..3107
                     /locus_tag="VQ516_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2512075.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            3206..3427
                     /locus_tag="VQ516_001672"
     CDS             3206..3427
                     /locus_tag="VQ516_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2512076.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3444..3758
                     /gene="trxA"
                     /locus_tag="VQ516_001673"
     CDS             3444..3758
                     /gene="trxA"
                     /locus_tag="VQ516_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2512077.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            4065..4598
                     /gene="queF"
                     /locus_tag="VQ516_001674"
     CDS             4065..4598
                     /gene="queF"
                     /locus_tag="VQ516_001674"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2512078.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(4852..5520)
                     /locus_tag="VQ516_001675"
     CDS             complement(4852..5520)
                     /locus_tag="VQ516_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2512079.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(5631..6185)
                     /locus_tag="VQ516_001676"
     CDS             complement(5631..6185)
                     /locus_tag="VQ516_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2512080.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPGFPKNRVHEVFEILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(6431..8227)
                     /gene="pepF"
                     /locus_tag="VQ516_001677"
     CDS             complement(6431..8227)
                     /gene="pepF"
                     /locus_tag="VQ516_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2512081.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            complement(8238..8981)
                     /locus_tag="VQ516_001678"
     CDS             complement(8238..8981)
                     /locus_tag="VQ516_001678"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2512082.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFITQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNVVPSIHLFENKADFLAQLDQ
                     FDRILVAYEEAAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(8983..9933)
                     /locus_tag="VQ516_001679"
     CDS             complement(8983..9933)
                     /locus_tag="VQ516_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2512083.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDMTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            complement(10071..10499)
                     /locus_tag="VQ516_001680"
     CDS             complement(10071..10499)
                     /locus_tag="VQ516_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2512084.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(10480..11568)
                     /locus_tag="VQ516_001681"
     CDS             complement(10480..11568)
                     /locus_tag="VQ516_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2512085.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(11587..12057)
                     /locus_tag="VQ516_001682"
     CDS             complement(11587..12057)
                     /locus_tag="VQ516_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2512086.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     VLFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(12348..12800)
                     /locus_tag="VQ516_001683"
     CDS             complement(12348..12800)
                     /locus_tag="VQ516_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2512087.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(12837..13016)
                     /locus_tag="VQ516_001684"
     CDS             complement(12837..13016)
                     /locus_tag="VQ516_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET2512088.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIGKQAGL"
     gene            complement(13153..13332)
                     /locus_tag="VQ516_001685"
     CDS             complement(13153..13332)
                     /locus_tag="VQ516_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512089.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            complement(13497..>13682)
                     /locus_tag="VQ516_001686"
     CDS             complement(13497..>13682)
                     /locus_tag="VQ516_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512090.1"
                     /translation="IFTKQIRHWILSDQVISGKRKLFFREDTPKEILDLYENIKSKLD
                     FAYQEVHSNNGLKKYEK"
BASE COUNT         4055 a         3116 c         2513 g         3998 t
ORIGIN      
        1 tggttgacgc tgaagctgat gcactggttg acgctgaggc cgaagcactg gtacttgctg
       61 aagccgacgc actggtgctt gctgaagccg aggcgctagt gcttgctgaa gccgacgcac
      121 tcgttgatgc tgaagctgac gcactcgttg atgcagattc agacgctgag gtacttgctg
      181 aagctgaggc actcgttgat gcggaagctg acgcactcgt tgatgctgag gctgaggcac
      241 ttgtacttgc tgaggctgat gcgctggttg aggccgattc agaagctgag gtgcttgctg
      301 aggctgaggc gctttgacta gcacttactg agagtgactt agactgtgaa agtgactgac
      361 tggtcgtgct agattcaact acctttcctc tacccttgaa gtagttagtt ctaatgttat
      421 ctgttttagc agcgtaaggg gtaaatgtaa aggatgtatc cgttccagta attggtacag
      481 tccaagatga tgttaatcca tatccttgct tagcaaagaa accattcatt tgggcaccat
      541 ttccccaagt atacccacta ccccacgtcg tcattgtaga tgtattatag gatcgaacca
      601 gtacaccatc tttgtcagta atgtagttct ttacatctga aggggtacca agatcactgc
      661 caagggttaa cattgtttgt attgaagtac ctgtattata gatagaatac ccatgaccca
      721 tctttgaaag attaccaaga gcccttgtgg taggatctac atacgtaacc gtataggtaa
      781 aggtcagttt tgaaccatca tttgtcactg tcaatttata gtaaattgga acatgatata
      841 ccgaatctaa acctgtacct gtataaactt ttgtctcact tttagcaatg tttagacttg
      901 cattaattgc aggggcgcca tttacaatcg tattgccaga aaaaacagca gcttttatag
      961 aagccagcaa ttgctcgatg gaatccactg aacgcttgcg tcgcttagca taagattgga
     1021 gattgacatt tgtaactgat gctgcataat cactagctgg tttcttacga tcttcttcga
     1081 ccttcttagc agttgcttct gtagcggcag ctgtttgtga tcctaccaca gtagatgatg
     1141 cagagatact tgtcgaagcc gatgtacttg ctgattctga tgcacttgta ctcgctgagg
     1201 tcgaagcgct ggttgaggct gactcagatg cagatgtact tgccgactct gaagctgaca
     1261 aactagttga actcgctgaa ttacttgtag atatcgtacc tagaactact gtatcatttg
     1321 ttgccaaagc atccgttttc tctacagttt tttcaagtac tgccgactca tttgtaaata
     1381 ccttcgtttg agttgcaacg gttccactta tgacagttcc cgcagcaact attcccttga
     1441 ggatatcaag cccagtaatt gaatgactta ctttatcttc taccgtttcg gtcatgacct
     1501 gagtagtatc aacaccacca cgcatgacct taaacaagcc aaaaagagaa gtcgaggccc
     1561 gtagccaatg cttccctgat ttgattaatt tgaaccgagt tactcggtct gtttctctat
     1621 attcaccctt ctgacgtcta aaaaatattt tgaaatttcc tttcgagtat tattttcatg
     1681 tatggatata aataaattac catactataa ttatagaata gggaacccaa aatagcaaga
     1741 aacttgaggt atttttgcta aaaaaaaaaa agcattttac tattttatat atatattata
     1801 agataagttt tttataaaga cacgcaaatg agtataaatc agctaatttt tggtttatat
     1861 tcctattttt atgcataatt attctttcat tctctaattt gtgaatcaat atatctgatt
     1921 agaaaactac cattgagttt ttgctccacc tcattagtcc gctcaaaacg ccgtccgcta
     1981 atcttgtcat agactttctc ccctctagga gcccctgcat aaggacgata gaggtagcgg
     2041 ttgattcccg tttcgtcaat atataggact ggtaaatctt ttaggttatc caaaatatca
     2101 agaaactcag aaactttctc aggcttttgt tccctaaaac tggtcgtctt ttttaaaatg
     2161 accttaatct gctttaaaac tgcccatacg gagggcaaag cacaatcaaa acgggccgca
     2221 atttcccgta aaaaagcgtc tggatgagcc tctacaaagg ctttcaattc ttctaaaggg
     2281 atcttacgct ttttgacgac tcgctttttc cgctctaagt tcccttgttt acgtctttct
     2341 tttcccacgt gaagagagtt ctgacgccaa caccaaaaaa cttggctgcc tcgacatgac
     2401 tgtgcccctc tttgatggaa tctaatgctc cttgtttaaa atcagtacta tatgccatag
     2461 ctttattaga ccatgcttat tttaaactaa gcaactatag atagagaaat gaaataaaat
     2521 ctaaattcga atgtgataag gagttagaaa ttgaaacaat aggattcccc cattattcac
     2581 acaaaaataa aaccactaaa aaacagagct tgccagcatt cttgaaaagt agtataatta
     2641 ttgcatgcgc aatcatcttg tgacgcagag actgcccgtt aatggacttt cttctatatt
     2701 atgactctaa aaagaaagga gacactatga cctatttgga aaaatggttt gacttcaatc
     2761 ggcgtcagaa agaaattgaa agtctcttgg aagagaccat tgcccaacag agcgagcaaa
     2821 gtctgacctt gaaagagttc tacctgctct actatctgga cttggctgaa gaaaaatctc
     2881 tacgccagat tgacctgcca gataaacttc atctgagccc gagcgcagtt tctaggatgg
     2941 tggctcgctt ggaagctaaa aattgcggtc tactcagtcg catgtgttgc catcaagata
     3001 gacgctctag ttttatctgc ctgacaaatg atgggcaaaa gacactggcc tcgctacaaa
     3061 agactgtcga agaaagcttg gaagcaggtt tggatttctt gatttaagat gattttaaaa
     3121 aaagttgcgt gcgcaatcat ttttcttgac attctctttt ataaggagta aaataaagtt
     3181 agcattaaac aaaggagttt taaatatgat tgaaattacc tatatagatg ccagcaagaa
     3241 cgaaagaact gtgactttcg agtcttatga agactttgaa cgttcacaac aagcttgcct
     3301 tatcggcgtc gcagactact accctgtcca aaaattaact tacaagggtc ataatttgga
     3361 ctaccatggg acttatggag atatcttctt ctatctcatg aaacaagatt taagccaata
     3421 taactaaaaa aaggagaaat acaatggcaa aagcaattac agatgcaaca ttcgaacaag
     3481 aaacaaaaga cggtttggtc ttagtagact tctgggcaac ttggtgtggt ccatgtcgta
     3541 tgcaaggtcc aatcttggac aaattgtctg aagaactttc agaagatgtc ttgaaaatcg
     3601 ttaaaatgga cgttgatgaa aatccaaaca cagctcgtgc ttttggaatc atgtctattc
     3661 caactcttct cttcaaaaaa gacggccaag ttgtcaaaca agttgcaggt gttcacacag
     3721 cagaacaaat caaggccatc attgctgaat tgagctaatc aaacgagaga ccaagtactt
     3781 tctttggtct cttttttctt gccctttgcc atttttcaaa aaatatgcta gactatagta
     3841 gattgaaact agaatagtac acctctactt ctaaaacatt gttaaaaatc gatttgactg
     3901 tcctgatcga tttgtcctgt ttttatttca ttttactata taagtagaat attacgatgt
     3961 ttgggatatg ccatcgctaa aaaaaaactc gatttggtat tttttagttc cctaatggga
     4021 gctttttgcg tgctctgaac atttcccatt ttggaaggag tactatgaaa catcaatcag
     4081 ccttggtcgt ctttagtggc ggtcaagatt caacaacctg ccttttctgg accatgcaac
     4141 actatgaaac agtcgaagct gtcacctttg cctacggcca acatcatcac ctcgaaattc
     4201 aaattactag agaaatcgct aaggaacagg gcattcgtca ccatatcctc gatatgtctc
     4261 tgctgggaca aatcactgct cagccagact ttgcgacgat tcatatttcc tacattcctg
     4321 acaagctctg tgtcgagtca aaatccctca aactatatct atttagctac cgaaaccacg
     4381 gagatttcca cgaaaactgt atcaacacca tcgggaaaga cttggtcaac ttgctagacc
     4441 ctcgctattt agaagtctgg ggaaaattca ctccgcgcgg tggcatttca atcgacccct
     4501 actataacta cggtaagcaa ggaactaagt atgagggctt ggcagaacaa cgcctcttcc
     4561 aacacgacct ttatccagag aaaattgaca accgctaaag tcatactcaa tgaaaatcaa
     4621 agagcaaact aggaagttag ccgcaggtta ctcaaagcag tgtttgaggt tgtagataaa
     4681 actgacgaag tcagatcaaa acactgtttt gaggttgtgg atagaactga cgtagtcagt
     4741 aaccatacct acggcaaggt aaagctgacg tggtttgaag agattttcga agagtatcat
     4801 acgaaaaagc cctgttcctc aagatgagga gcaaggcttt ttgagtttca attattcttc
     4861 tgttccaagg aaattttttg caacaagggc tgcaagaact ccaccagcga ttggtgcaag
     4921 gatgaaaatc caaacttgtt gaagggctgc gccgcctacc aagacagctg gtgccaagct
     4981 acgagctggg tttactgaaa gtccagtaat cttcaatccg acaagaatca tcgccatcaa
     5041 tgacaaaccg attaccaaac cagcaatcgc gccattgccc ttgctttctg aagtcacagt
     5101 catgataacc aatacaaaca agaaagttgc gatgacttca aacaagaaac caccaaagac
     5161 agtgacaccg tttgccaagg cattttcacc aagactagca gttgacatac ctgaattact
     5221 caagaggaag aagactgcgc cagaagcgat gaaagctcca acaacctgac caaggatgta
     5281 gtttacaagt tctgaagatg acaaacgttt gtttacaaac atagcaatcg aaacagccgg
     5341 gttcaagtga gcacctgaaa cagttccgat tgagtaggct gccaccacga ttgccaaacc
     5401 aaaggcaaag gcgattccaa ggtgaccaag gccatcaaga ccatttccaa aaacaacagc
     5461 tcctgttcca acgaacacaa gcatgaacgt accgattaac tcagcaacaa attttttcat
     5521 tttctttctc cttttttcaa aaactagata ctagtctatc aaaagtagga aagggtttca
     5581 agaaaattga ttggaaattt tttgaaaatc atagaactat tagctaatct ctagtattga
     5641 aaagactgga tagcttcttt caactcatct tgtaaactat ttctctggtc aagttggaca
     5701 tagacttcca ccagacagga tctaaagttg gaaaatttgt aaaaatcctc cctttcttct
     5761 atcggaaaat caacagtttt tatccaagaa gctacttgtt cttgcgctaa cttcccttgt
     5821 aaaataggtt catagatcac tcttgctaaa cgccaatcct catcatctgt aaagcgaatc
     5881 gacattcttt taaataattg gccaagtatc tcaaatactt catgaactct gtttttagga
     5941 aagcccggat gacaaaccac ctctgtcagt aaatcggctc catgtgcaaa agcatgaacc
     6001 caaccatact gacttgaaaa accccttgta tccttttctt ttgaaagata gtgcaagcct
     6061 tgatttaaaa ggacattacg aatttctgga gaaggatttc ccaaatgatc aaacaaccac
     6121 tggatttctt cctggttata atttggtttt tcttctgcta tttttcttag taaatcttga
     6181 tacatggtca atacctctac atttctagca actgttcaaa aaggcagtct taaatgactc
     6241 aatattgaat tctcaattaa atacaatctg atataaaatg acgtaaataa ctatcaatac
     6301 cagttctaca gtaagttcaa atttaacatc acgatcttca acgacatttt tgaaaatagc
     6361 tacaactaag acaaatagaa tgacgcttaa caagcccata aacatcattc taaaaaattt
     6421 ttctattccc ctactctccc aactcagcac tataggagat aatctggtca actgtgtcag
     6481 acaagaattg gatggtatca cggagtggtt tgtctgttga aatatcagca ccgataatca
     6541 tggctgactc aagtggtgtc ttgctaccac ctgatttgag gagattgagc cagtcttcag
     6601 ctccagtttc agaatgtttt agatgaaggt aaccagcagt cgagataact agtcctgctg
     6661 agtaagtgta actatacaag cccatatagt agtgagcttg gcgcatccaa gtcagagttg
     6721 catcatcgtc aatttcaata gcatctcccc agaaatccgt caaaacttcc ttcataatgc
     6781 tgttgagctt gcttgctcca aaggtctccc cttcttcaat caatgtatac accttacgct
     6841 ggaaggcggc ttccaagagg tgggtgataa agttatggaa gtaggtgtct gtcaagcgat
     6901 gagccagagc gaagcgtttt tgacgtgggt cattagactg gttctccaag taatcactga
     6961 gtagcaattc attgaaggtt gacggtgctt caacatagta ggtcgacata tgggcattga
     7021 agtaactttg atgattgtct gaaaagatga attgaccaga atgcccgatt tcatgaatca
     7081 aggtatagac atcgctcaaa cggcctgtcc agctcatgag tacataaggg tgtacgcgat
     7141 atgggtccgc cgcataacca ccggaatcct tgccactgtt agcagcaaag tccacccagc
     7201 gctcttcttg gtaacgagca acttcctgac aatattcttg ccccaaaggt tctaccgact
     7261 tcatgaccaa atcataggca tcgtcaatag tcacttcagg attcagggcg ctgtccaagt
     7321 ccaatttcca gtctgcaaag gtcatctttt caagaccatt taccttggca acatgcttga
     7381 ggtatctctg agcgactggt gcaaagtcct tcatgatgag gtcaatctgg cggtcaaaca
     7441 tgacacggtc cacttcttgt tcagctagaa gatagtcaaa gacagagtcg tatcccttca
     7501 tatcagccaa gagtttttca gacttgacct gagccagata ggctgctgca gccgtatttt
     7561 ggtgcttacg aagtccctct gagaaggaac ggaaggattt ctcacgaacc tcagcatcct
     7621 catggttttg gtagaaattc tcataggtca caaagctgtt tttgtaggtc ttgccatggg
     7681 cttcaaagtc agccatttca aaatccccag ctcgcatctt agtataaatg tcctgcggac
     7741 tgtagaaaac ttcaccgaga tttgtcaagg ccttctccac atctgcccct aagtagtggg
     7801 cttttttgat tttagcctga cgaatggcag ctgttaaatg tggcaattta cccaaacgat
     7861 ccaagacttc ctcgtctgct gccaccaagg catcgtcaaa aaaggtcaag gctacgctgg
     7921 catctgtttc aaattccatc ccagcttgag caatatttgc aaattcgtca ttgctatagt
     7981 ccgtcgtctg aggcataaaa ccatagttgc caatatggct catctgaatg tagatctgtt
     8041 ccaattccgc aaaggccttc tcgaaatcct caaaagtgtg aagattgccc ttgtaatcac
     8101 ggctaaactg gttgatgtct tcgcgagctt tctcgattgc acgcaagaaa tcctcacggt
     8161 cttggtatag agctgttaaa tcccaaagtt ccttctctgg aaattctgaa cggtgttttt
     8221 gttccatttt cttcctctta tttctctaat tctactaaaa cactaagggc tgataaggcg
     8281 taaagcggtg ctgtttctgc tcttagaatg cgagggccaa gtcctgccaa aacagctccc
     8341 ttagcttcaa aattttcaat ttccgcaggt gacagaccac cttctggacc aaagataaag
     8401 agcaatttgg ctcctttctc gagtccagta actgctttta taagagcagc ggcttctcct
     8461 tctttggccg cctcttcata ggctactaag atgcggtcaa actggtcaag ttgggctaga
     8521 aagtctgctt tattctcgaa aagatggata cttggaacaa cattacgctt actttgctct
     8581 gctgctccaa gagcaatttt ttctagtttt tcaacctttt tacccaattt cttgccatcc
     8641 cacttagcaa cggaccaatc ggcagggaag gcccaaatct ggctggcacc tagttccgtc
     8701 accttttgag tgataaactc caacttatct cccttgggaa atccagatgc gatggtcact
     8761 tggactggta gttccacatt gtcatttaat tcttggacca actcaaactg acgattttcc
     8821 atatccagca cgcgcgccaa gcgcttgatg ccatcatcaa agactaaggt aacctcatcc
     8881 tcttctttca agcgcataac ctgaaacata tgcttgctgg tttccctgtc ctcgatggtg
     8941 acaggagaga ctgcactccc cttgacaaaa tactgttgca tgctagcctc caatcacacc
     9001 tgaaatatcc ttggtcttct tgaagacgca ggcattccat tccccttgaa tcatatgggt
     9061 ttcaaggaaa aatccagctg actcagccga ctggcgcacc atgtcccact tgtccttgat
     9121 aatgccactc atgatgaggt agccttcatc ctttaccaag cgataagcat cctccgtcag
     9181 atgaatgaga atatccgcca agatattagc cacgatgaca tctgcttcaa tctcaactcc
     9241 cttaagcaag tctcctgctg ctacatggat attttccata ccagtgttga gctcaatatt
     9301 ttcctgagcg acacgaaccg ccacatcatc caagtcatag gcgaaaatct ccttggcacc
     9361 taggagtgag cttgcaatag aaagaacccc tgaaccagtc cccacatcta gcaccgtttc
     9421 gccaccacga agaacctgtt ccaaggcaaa aaggctcatc ttggtagttg ggtgggttcc
     9481 agtaccaaaa gccatgccag gatccagctt gataattttt tctcccgcag tcgcctcata
     9541 gtctgtccaa gagggaacga tggtcaaatc atgagtgatg cgagcaggtt catagtattt
     9601 cttccagttg tctgcccagt cttcctcagc caaggcagtc gtacctattt ttaactctcc
     9661 caaatccata aaatccgtca attctgctaa gcgagcctgc aagtccgcct caaccactgt
     9721 catatccacc gtgtcagggt agtaggctgt cactacgatt tcttcttgct gctccacctc
     9781 tgggaaaatc tcgccaaaac gatccacatt tcccatatag tccatgctgt cttcaatcgc
     9841 gactccttgc gctcccagct caatcaagag attggaaacc aactcctctc cctcacgctt
     9901 cactgtaact tttaactctt gccatgtttc cattattaag ataccaagcc cgtaaaacac
     9961 aaaaccaaaa taggaaattc tctgaagacg cttgtgtcta agagaagttt atctttttgg
    10021 cacagtgttt agggcgggtt cagtttagaa atgtaactga accatccttt ctaatcactt
    10081 acttttaaat aatcttttaa tctctcttgc aactgaggca caacttgact ggaactaaga
    10141 aattcctcaa cattcatcag ctgatagccc tgtccttcat ctccgaagat gatattgtca
    10201 aattgttctt gtcttagctg accaaccata aagaccgatt tcttgccttt aaaaattacg
    10261 ctaggataaa tcttgctcca aagcagacag tcttcatcta aatgaattcc cagttcctca
    10321 taaacttcac gccgagcgca ttcaaaaggg ctttcgtccc cttcacggcc accacctggc
    10381 aactcccaca tgttgggaca agggatatcg tccttgtcat cacgcaaaat cgtcagtagc
    10441 ttatccccac aaaagagggc aatcttgcaa ccttggaaat cagaaatttc tagttccatc
    10501 ttcagttcct tctaacattt ccttttccag ctctgctaac aagttttcat aatagcgttt
    10561 ctcgtccctc aacattcgac tactatccat tttctgtcta gcaatcttga gagccttacg
    10621 agttcgatct acatctttct tcacctttaa ttgataccag gcttgtatca cttgaagatt
    10681 ggacagtttg agagacagaa acgatttgac ctgtcgaata ctagcatatt gctccgcttg
    10741 ctcaaaatct ccttccaaca aggcgatatg aagcagggat agttgggcaa ctgtctgcat
    10801 catcggagta gttgtcctct caagtaatgc ttgaaactgc tgtttagcta cttcttcctt
    10861 cccttccaaa atggaaactt caccttgcat acctaataca ccatccgcaa aactccctcg
    10921 tgcatcctca ggaactgctt gaacaaagtc tttcaaatca tattcttgag gagccagcaa
    10981 agtttgagca gaatgtctca ataccaggta ggcgtatttg gtattttcag ggtgttgtag
    11041 taattcccaa atttttgctc catcggtgat gtcgactggc aaaatgttat ttaggaagaa
    11101 agataaatta agaaaaatcc aagtccctgc aaaataccag cttcttgtca aaaatccaaa
    11161 cactatcgcc aataatatca agccgagatg aaccatcaag cctcctgaaa gcatcaggat
    11221 gattctttga tcgctttcat cctcttttaa accaatgtat tgagcaccaa catttttcag
    11281 aatggctgtt ctactaagat gaaacctgcc tgactttttg gtcaaaataa aatgtcctaa
    11341 tccaaaagcc accagccgat agcctgtcaa gtagccacaa aaagcatgac ccagctcatg
    11401 aagaataaag attaaataca tgcttagaag agcgaaggca taaccaaaag taaaggctaa
    11461 aactgcggaa taccccaact ctgcaaatgc gattgttcca caagcaaaag ctagcataat
    11521 aaagacaaca gctagcacat aaaccaaata agtcccaatt ttcttcataa cacctccaac
    11581 caactcctag tatcttggat aaggataaaa ttctccctct tccaagccaa cttttccttc
    11641 ttcaaagact tcttggttcc attccatgac aaattcctct gcttctgggt cttccaaaaa
    11701 gtccatgagg acatctagcc caacctcagc agtatcttta aggaaaagca caaaataagc
    11761 taaaaattca cgggaaaatc cttttttagg caggtaagga ataacagtca aatagtcttc
    11821 ctcattgact gttgacttag caggattgta gaaaaggacc gcttcctcaa aaagaatgtc
    11881 atctgatgaa acctctccgt cttcatccac catctccaca cctgcagcat tttgcgcttc
    11941 caatagaaaa ctcacttcta ccgcatggtt gcgtttgtcc cagctaatct caaagtcaaa
    12001 gggaaagttc ttgtccaact cttcctctaa aacatctaaa aatccgtatg ttgccatttt
    12061 gtcctctttc tatgcgactc tttaatcgcc ccgattgctc ggaaatatgc taaaatagat
    12121 actaccatct taccacaaaa ttattttatg tcctaattat accatattac ctcatttaaa
    12181 cccttggtat cagtgatttt cttaaaagtc tgatttcttc atttctcata aaaatcaata
    12241 taaaaagccc tcgaaagggc taataaatct ataaaatcaa taggcgagta actagcacaa
    12301 gtggacgtgc ttttttattg actattacca cgataccacg cttaatctta ggcttgaact
    12361 ttcttatctg caatagcgtc tgtcaaagtc tgagaaaagt taagtcccat ttctcgtccc
    12421 aacttatctg cccattttgg tatggtcaaa gtctttttaa tgggttcctg acttcctagg
    12481 tattctgata catcaacaga taccatagaa ataaaagatt tatcaaggtc ataggttgac
    12541 acgaaatctt catcatcttt aaaaggatca ttatcaatta aagacaagct attgatatct
    12601 gatggctgag gtaactctcc atcactctct atcaaatctg caacagttat ccctagccac
    12661 tccgacccca tagccaaagc ctcagaaatc ccctctcctt gtgtagctga gtattcaaaa
    12721 tctgggaaat ggacaaaata agtcgcttct gttccgtctg tgtcgtcata ataaaataaa
    12781 gctggatacg taactaacat ttcactacct ccatatcaaa aagcagggac tgaattttac
    12841 aacccagctt gctttcctat ccctctttca gtgtacttat tcagctcacc atgaaggatt
    12901 gtgataggtc tttccccttg cttctcgatt ttaatatggg agcctttacc gcctctagtc
    12961 tttatccaac catgggccgt aaagagttta accatctctt tttgtgtcat aggcatagcg
    13021 cttttacctc ctgacaacac cattataaca cgtgttacac gtattgtaaa ggagtgatac
    13081 ttattattct attatacata aaagccccta gatgtggttc taagggaaac caatttattc
    13141 agacctattt ttctaatgag tagtaaaaac tgcttcttta tcgagcaatt catcatctgt
    13201 atagtcaatt gtaaaagtat ctcgatctaa gacagattga ggcggagttg aatgaatcat
    13261 aggaccactg cgtactctat attttttatc tccaattttt acaaactgat actcttcgaa
    13321 aatcaaattc aaaccacgtc aacgtcgcct taccgtactc aagtacagcc tgcggctagt
    13381 ttcctagttt gctctttgat tttcattgag tatgattaac tctcaagtct tcgaaatcag
    13441 gattttcaac agttattaca aggaggcgat ttactacttc aaaaacatca attattctat
    13501 ttttcatatt ttttcaaccc attattagaa tgaacttctt ggtaagcaaa atcaagttta
    13561 gatttaatgt tttcgtacaa atctaaaatc tcttttggag tatcttcccg gaagaaaagt
    13621 tttcttttcc ctgaaataac ttgatcacta agaatccaat gacgaatttg ttttgtaaaa
    13681 at
//
LOCUS       DAVBCV010000056        12693 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBCV010000056 DAVBCV010000000
VERSION     DAVBCV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.056"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            2..1242
                     /locus_tag="VQ516_001687"
     CDS             join(2..341,341..1242)
                     /locus_tag="VQ516_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2512091.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
     gene            complement(1477..2085)
                     /locus_tag="VQ516_001688"
     CDS             complement(1477..2085)
                     /locus_tag="VQ516_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET2512092.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            2251..2421
                     /locus_tag="VQ516_001689"
                     /pseudo
     CDS             2251..2421
                     /locus_tag="VQ516_001689"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            2410..3021
                     /locus_tag="VQ516_001690"
     CDS             2410..3021
                     /locus_tag="VQ516_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2512093.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
     gene            3738..4424
                     /locus_tag="VQ516_001691"
     CDS             3738..4424
                     /locus_tag="VQ516_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2512094.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMAAISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            4425..5357
                     /locus_tag="VQ516_001692"
     CDS             4425..5357
                     /locus_tag="VQ516_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2512095.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFFFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            5368..6297
                     /locus_tag="VQ516_001693"
     CDS             5368..6297
                     /locus_tag="VQ516_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2512096.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            6311..7750
                     /locus_tag="VQ516_001694"
     CDS             6311..7750
                     /locus_tag="VQ516_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512097.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            7819..9396
                     /locus_tag="VQ516_001695"
     CDS             7819..9396
                     /locus_tag="VQ516_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2512098.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            9456..10916
                     /locus_tag="VQ516_001696"
     CDS             9456..10916
                     /locus_tag="VQ516_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2512099.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNYMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            10938..11393
                     /locus_tag="VQ516_001697"
     CDS             10938..11393
                     /locus_tag="VQ516_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET2512100.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            11480..11885
                     /gene="cdd"
                     /locus_tag="VQ516_001698"
                     /pseudo
     CDS             11480..11885
                     /gene="cdd"
                     /locus_tag="VQ516_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            11898..12347
                     /locus_tag="VQ516_001699"
     CDS             11898..12347
                     /locus_tag="VQ516_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2512101.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDILKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEETILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHRTGLLVEEPLNIKKVVFKVKI"
     gene            complement(12459..12693)
                     /locus_tag="VQ516_001700"
                     /pseudo
     CDS             complement(12459..12693)
                     /locus_tag="VQ516_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4517 a         1652 c         2339 g         4185 t
ORIGIN      
        1 catggtgaaa ttaattgacc gttatggcat agaaatcgtt aaaaaaggga agaatcgtca
       61 ctattcttct aaattgaaac aagaaatgat ggataaagcc ttactggaag gttgttcaca
      121 aagaagtatt agtcttgatt atgccctccc aaaccaagga atgctttcat tttggccggc
      181 acaatacaag aaaaacgggt atactattgt tgagaaaaca agagggagac cagctaaaat
      241 gggacgtaaa cggaagaaga cttgggaaga aatgacagaa ctagaacgac tccaggagga
      301 gaatgaacgc ttacggactg aggtggccta cctaaaaaag ttaaaagagt tagaggaaag
      361 ggacgaagcc ctagagcgag aaaggcagag acagttagag aaatggtttc aggaggattt
      421 cgactagatt ttcttcttga aacagcacgt ttagctcgct cgacttacta ttatcagttg
      481 aaacaactgg atggggttga taaagataaa gagattaaaa ccgaaattca gggcattgat
      541 aatgaacata aaggcaacta tggctatcgt cgaatccatt tagaactaag gaatcgtggt
      601 tttgtggtca atcataagaa agttcaacgg ttgatgagaa ttcttggttt aacggctcga
      661 attcgccgta aacggaagta ttcttcctac caaggagaga ttggcaagaa agcagagaat
      721 ctcattcaac gccagttcga agcatcgagg ccaatggaaa agtgctatac agatgtgaca
      781 gagtttgcca ttccaaatag cacacagaaa ctctatttat caccagtttt agatggtttt
      841 aacagtgaaa tcattgctta tcatctttct acttcgccaa acttagaaca agtgaagagt
      901 atgttagaac aggcctttac agagaaatac tatgagaata cgattctcca tagtgaccaa
      961 ggctggcaat accaacacga ttcttatcat cggttcctag agagtaaggg aattcaagca
     1021 tccatgtcac gcaagggcaa cagcccagac aacggcatga tggaatcttt ctttggcatt
     1081 ttaaaatccg aaatgtttta tggctatgag aagaacttta gatctttaga aaaccttgaa
     1141 caagctattg tggactacat tgattactac aacaacaaac gaattaaggt aaaactaaaa
     1201 ggacttagtt ctgtgcagta cagaactaaa tcctttggat aaattatttg tctaattttt
     1261 tggggtcagt acagtattaa ataccatgat cttatgcgtt ttataataaa aaatctgttc
     1321 ttgttttatt tttagaaaat ttactaaaga ctaaaaatat tatgaataat ttaaaaaata
     1381 gtcaggttat atagtttttt gaaattaaaa ttattttatc gttgaaatta gttaaaaatt
     1441 tttataaaat caagatgcaa ggctatgaga tgttctctag tttaattttt catacttcga
     1501 gaatataaag ttatataaaa atgcattagc atattttgtt attgagggaa gtataaaaat
     1561 cattaaagtt atccaaacta gtaattgatt ctgtaatata aagaagagta gaactgggat
     1621 aatttcaata attctaataa gaacggcaat aaaaaaatgt ttataggcta acatgattga
     1681 attgtgtaga gaatttttta aagtgttatt atatctaccg attaaaggaa ttagaaatgt
     1741 tgttgctatt aaatgaagta tgatatatat tactccaata tgtcgtaaga tagaaatgat
     1801 atatataggt aatggtatgt agggtaggaa gaacaaactg aataataaga ttaaaattgg
     1861 taaattagat attaatgctg ctcctaaatt tttccaataa tctttaaaaa aactgtttgc
     1921 gaagtctcta tagggatagg attcctcgtg tcgtacaatt tttattagag ttgctattag
     1981 ggcactttca cctgatccga tagcaataac tgttactact gacgctacat agagtagatt
     2041 taacattata agatttgaaa ctttatcaat aaattttatc tgcatcttta actcctgctg
     2101 tttttatact tataatatca catcatttga gtttcatcaa ctttgaatac attaagaaag
     2161 aatataacta tgaggaaagg aaaaaattat tatgaataaa tccaaaaaat ataaagaaag
     2221 aattcattat gaatacgata atcttactaa atcagagaaa aaattgctga ctatatttta
     2281 gttagagata gtgaaatagt ttatgaaacg tcttcagaaa ttgttagtaa agtaaaagtt
     2341 ggagagggaa ctcttaatcg attttgcaaa aagattggat actctggttt tcaggaacta
     2401 aaattaaaaa tgacgaaaga tatattagag ttagaatctc aaaaaatgag tagtgacaca
     2461 tttattgatg aaataaaaaa taattattta tctattgtgg aaagtacaag aaaattaatt
     2521 gatggtagac aaatagaatt agctataaaa ttaataagag aagcaaacca gattttaatg
     2581 attggtgtgg gttctagtgg aaatgcggct agagaatttg agagtagttt gttaagaatt
     2641 ggaataataa gtaagacggt aattgatacc cattttcaac tcatgcatac tgcgttattg
     2701 aaagataatg atttgataat agcgtttagt ttatctggtt ccacaaaaga agttgaggaa
     2761 actttattaa acgcaaaaag aaaaaatgtt aaaattattt caataacaaa ttattcgtcc
     2821 agaaatatag caaaattatc tgactgcgtt cttttgacat caaaaaaaga atcttattta
     2881 gagggtggta gtttaatggc taaggcttct caactattca taattgatgt aatatgcact
     2941 agactctctt taataaatta tgaagatact atatgtaaaa aagaagagat tgcttctttg
     3001 ctgtcaaata aagtggaata aatcaataat ttttaaattt taataaaaat tttttaaaat
     3061 aaattaaaaa acgatcctaa aagggtcgtt tttttgctaa agttttaaaa ataaacaata
     3121 attaagatta tagtgcgttg aatctatatt aggacataac aattcttaaa tcattgttag
     3181 aaattagttt gaattttcca atcaatttgt tcgattttta catcattgca ttatatcacg
     3241 tgactattaa ccttgttttc tcctaaaatt gattttcttc ttttcttatc ttgtccactc
     3301 gaaacaagta ttgtaagaat ttgattattt ttgaaagtac ttttaatata tttgagatag
     3361 ttaaaaaaga tttgaaacta aattccaaat tagaaaaaga cttgaaatgc taaaaaaaaa
     3421 aagtatactc taattgaaaa cggtaacaaa actaatttag agaatgaaat atagagtatt
     3481 tctctcttaa aagtttttgg tgaaacgaga tgtagaaagg agatttagcc aaagagtcta
     3541 ttagtgctag aataatagat tagaattata gtgtattgaa tctataacag tacgccttaa
     3601 atgctaaaat atttctataa attaatttaa ctttcctgat agggctgttc atatcttatt
     3661 tcaattcact atataaataa aattgtagta gatgttattg aattagtaaa aaaaataaaa
     3721 aaacggaggt tttctgaatg gaaactaaaa aaattaaaaa tttaaaaggt caaataattg
     3781 tttcctgcca agcgttagaa ggagagccac tttatactcc aaatggagga gtgatgccgt
     3841 tattagcaaa agccgccttt caagcaggcg ctaaaggtat tcgtgctaat tctgtccgcg
     3901 atatttcaga aataaaagaa gaagttgact taccaattat aggtatcata aaaagagatt
     3961 acgatggctt tgaacctttt atttcagcaa ctatgaaaga gattgatgaa ttagttagtg
     4021 aaggagtaga cattttagct ttggattgta ctaatcgttc aagaccaggt tatgataata
     4081 ttactgactt tattcatgat attaaagtca agtatccaaa ccaattatta atggctgcta
     4141 tttctacttt tgaagaaggg aaagtagctg ctgaatcggg agtagatttt gtgggtacga
     4201 cattatctgg ttatacacca tatagcccta aaaaagataa tcctgatttt gagttagttg
     4261 aaagattggt aaaagagctt gacgtaccag ttattgctga aggaagaata agtactccgg
     4321 aacaagctag aaaaatgtta gatttaggtg cttacgcagt ggttgttgga ggagctatca
     4381 ctcgtccttt ggaaatagcg aagaagttta ttgaggtagt ttagatgaat caagaaatat
     4441 taaaaaagag taagattcaa ttgacagttg aacatattca aaaatattgg attttatatt
     4501 taatgatgat tccagcaact gttctactaa ttttatttac gtatggacca atgtacggca
     4561 ttattatggc ctttcaagac tttactgtct tcaagggata tactggaagt ccatttgtcg
     4621 gtttaaaaca ttttcaaaga ttgttctcag atcctttgtt ttatagattg ttcaagaata
     4681 cgttcatggt aggggtttta gattttttat tctttttccc agctccgctt atttttgcac
     4741 tcatattaaa cgaagtcaga aaagtaagat ttaagagtgt agttcaatct attagttatc
     4801 ttcctcattt tattccccta gtggttatgg ttggtattat ttttgaacta tttggttcat
     4861 atggtattat taacagttta ctttcatcac ttggaatgga gccaataagt ttctttacaa
     4921 aatctgaatg gttcttgcct ctctacattg gttcaggagt atggaaaaca ataggttggg
     4981 gttctatcat ttatatggga gcattaacca atatagattc aactttatat gaagcagcag
     5041 atatggacgg ggctaataga tggcacaaaa tgtggcatgt aactctacca agtttaagac
     5101 ctacagtagt tactttgttt atactaaatg ctggtggtat catgcaagta ggatttgaaa
     5161 aggtattctt gatgtctagt cctgcaacat atgaagtatc tgatgtatta tctacttatg
     5221 tttatagaca aggtatttta aattctgatt tcagttactc agctgcagta ggattattta
     5281 ataacatagt agctttgtta tttgttttat tagctaataa gattgctaaa aaacttggcg
     5341 aagaaggaat tgtataaggg gggtaaaatg attttttcaa aaaatacaaa aaagagtaat
     5401 agagacaaag aaaccttcgt tgattatctc tttatattca ttttaataat aataacctta
     5461 cttatgttgt atccattatg gtgggtagtg atgacgtcac tttcagaacc agtttggcta
     5521 tctaaaaacg ttgttaaatt atttccctta aaaatagata ttactgcata taagtttatt
     5581 tttggggatt ctaaactatg gcaagcatat ggtaatagtg tattgtatgc tgcactatct
     5641 acaattgtta gtgttgcggt atgtagtatg tatgcctatc cacttacaat tcctgaaatt
     5701 aggggtaaaa aattctggaa tactcttatt ttgattccaa tgttctttag tggaggaatg
     5761 atcccgcttt atatacaagt tgcaaacctc ggtttattga atactgcttg ggctcttgtt
     5821 ttacctggtg ctgtttctac atattatata attttgttta gaacacagtt taaatcaatc
     5881 ccaatggagc ttcgtgagtc tgctagaatt gatggggcaa gtgaatttag aattttttgg
     5941 aatttagtat taccattatc aaaagttatt ttcatgatta tagcacttta ttgcatagtt
     6001 ggttcatgga atagttttac tcctccatta ctttatttga atgacgacac taagatgcct
     6061 ttatctattt atttgagaaa tttagtcgtt acaaatacaa ctgatactgc tgaattatca
     6121 ctttctgcag gtaatacata ttaccaacaa attgctgctc aaggtggagg tattggtatg
     6181 cgtattgcta ttaagatggg tactattgtt gttagtgtta ttccaattat gatgatttat
     6241 ccatttattc agaaacattt tgttaaggga atcctgctag gttctgtgaa aggttaataa
     6301 ggtaattatt ttgcaaaatt taaaaaaata tcttttatta gatagcggtt tagtagttgg
     6361 gtatacatta gaaaatgctc aattaaaatt aaataagcta aaaaaagata ctgaaaacaa
     6421 tccactattt ggtgaagagt attttgccga aaatcctaaa ttatgggaag ttcgttttga
     6481 taattcatat ccaaacgtta tctatgataa agaattgaaa ttatatagat gttggtatca
     6541 aacatttgtt agtgatgaag caagcgaaga gactccttta gcagaacgtg gagagaagga
     6601 atacattgtc aagtcatcta gaatgacagc actttgctat gctgaaagta aagatggagt
     6661 aaaatgggaa aaaccaaatc tcaatctagt aaaatttaaa ggttcaaaag ataataatat
     6721 tgttctgatg catgcacacg gtgcaggtat catgctagat gaacaggagt tggatcctaa
     6781 gaaacgatat aaattagttt caaaattaga attttctccg aatcttcatt acatggttgt
     6841 tgttttttca gaagacggta ttcattttga cgaacctatt ccatggaaaa aatggaatcc
     6901 tgcagcagat agtcataatt ttccatttag agatccaaaa actggtaaat acgctgttat
     6961 tacaagaacc tgggcaaata acgtacgtct aagtgcaatt actttcagtg atgattttat
     7021 taattggtca gaacctaaag aaatccttag gggagatgga tttgaagatc aaatttattc
     7081 tatgccagta atttcatacg cggatatcta tttaggtttt gcttcaatgt accatgaagg
     7141 cgacacttta aatgaaaact atgataaagt agacttagaa ttgaaattct ctgctaattt
     7201 ggaacaatgg gatagtgtat gtaagggcga ctatctcatt gaaaggggag gtggagtata
     7261 cccgcagaat gctgattttg atgctagttg tattttcacg gctgctccaa taattgaaga
     7321 tgataagatt tggttctact atatgggtgg aaatggtaat cacactggct tccgagaaag
     7381 ttcattagct agaggatata ttaagaaaga ccaatttgct tattatacta ataagagaga
     7441 aggaattcca gctaaaatat ctacagttag cttccaaact tacgatgatg aaatttatat
     7501 cttagctgat attcctgaaa atggttatat taaagttgcg gtaggtacta aatacggtaa
     7561 tgcttattca gatttcggtc aggaagatat ctctcttgtt caagtttccg atggtaagta
     7621 taagttggaa tttaataaaa aatctatctt agatttaggt aattctccag ttgcgttcaa
     7681 atttgaaatc gagaatgcaa aattgtatgg tattgaaggt agtattcagc aacactatct
     7741 caaatattaa ccagaataat tcgttaacaa ataatctcta tattagtgga gatagatata
     7801 taaaatggag gtcttattat ggtaaagctt aataaggtat taaaagggag tgttgcattc
     7861 ctgagtgcaa gtttattgct agcagcttgt ggcaataacg ctaattcaaa gcccgctgaa
     7921 aaaacagctg atggtaaaac gaaaatttcg tttctatcta catggcaaat taatgatgtt
     7981 cgtccgccta aagatgaaac acttgttggt caatggttag aagaacagct aggtattgat
     8041 attgatcttg taactattcc tggcgaggga gtaagttcta aagtaaattc tcttattact
     8101 agtaaccagt taccgactgt tttgttgaca acaggagata agagtgatat cgctggtatc
     8161 aacaaacttg gtaaacaggg tgcattctta gatttgtcac aacacatgga caaactacca
     8221 aactataaga agtaccttga aaaaaataat gcattagctc aaatagaaga tgatgaaaaa
     8281 catatttatg ctttcacaaa attctttact gatgagaata tcatgtatac tacaccaatt
     8341 ctgagaaaag acttacttgt tggttcagaa tttgaagatc tttcaaaaat taaaactgtt
     8401 gatgactata caaaagtttt gaaatatttg actgaaaaac aaggttctcc agcatttatt
     8461 caacgtaatg gctatgaagg cttcatgaag cgagttacac cattgtggaa tttaagccat
     8521 cgtacatact atgactatga atcagattca tataaacatc cagttgaaca acctcaattg
     8581 aaacaattcg ttgaatggtt gaaagaatta cgtaaagata acgtacttca ccctgactgg
     8641 gcagttatga aagatgaaac ttgggaaggt ctattggctt ctaacaaggg ttcattcact
     8701 gttgaccgta tgaatatcat tggagataat aactttgata aatcatttga ttggcagcca
     8761 cttgtttatc cagaaattaa tggaaaacgt tatttgcaac cgaaaacacc gtacatttct
     8821 gattcaggtt gggtaatcaa tgccaatgca ccaaaagaag aaattgataa agcgttagaa
     8881 ttctttaatt tcttatacgc tgatgaaaat caagaaaaac ttgctattgg atttgaaggt
     8941 aaaacattta cacgtgaaaa ccctaatact caagcaggta ttaaatggtt gattcaaatt
     9001 tacggtgaaa atgctggaaa tgataaagca gaactgctgt ttaaacatgg taaccaagca
     9061 tttactcgcg ttcaaactaa attagatatt gaggcacagc caggaacatt ccctaaagtt
     9121 atgtatgatc aagtagataa aattaagaaa gatttaggtg gttttagacc agatactccg
     9181 gttctttcct ttacaactga agaattagat gttttaacta aatctgaagc aggattaaat
     9241 actttctttg atgaaaatat tattaaattc attgaaggac aaagagatat gagtgagtgg
     9301 gattcattta tcaatgaagc taaatctaaa gacttggata aagttacaaa agtatataat
     9361 gatgcctaca aacgtgtcaa agatagaatg aaatagacta aatggtagct ctctggtttt
     9421 tcagagagct actactgcct gagggggagt aagctttgta tgatgtagta attataggtg
     9481 caggaagttc gggcgcattg gctgcaattg cttcggggcg tctagggtta aaaacttgtc
     9541 taatagagaa gggaagtaga attggtggag ttcctattaa tacactaatg gggtcatttg
     9601 ctaatctata ttttgatgat tctggtagag taaatgcaag taaaattgtt ggggaattaa
     9661 tagaacgtat tatcaaaaaa ggtggtactg tctttaacta tatggaagaa ttgatgaatg
     9721 taaatgattt ttatcagatt acaattccct atcaaccaga agtttatgaa tcggttttaa
     9781 ctgatatgtt gattgagtct aaagttgata ttttactaaa tgttgaagtt aataatatta
     9841 tttatgatag taaaaatatt aaaagtcttg aaattttaag taatcaggaa gttaaattga
     9901 ttgattctaa agtttttatt gatgctacag ggaatgcaac aattgcttct gctagtggag
     9961 ctgatgttta tgaaaaaaat tcttcttacg gttgtttaat gagagttgga ggagttgata
    10021 tctcaaagac tatagaacat attgaaaaaa caaagcagtg gaactatata gaaggatata
    10081 atcagtggtt agaagaagta ttagcaaatc gaaagtacag agccactgga gcagttttaa
    10141 aagatccagt ttcttatgat catgccccta tgctttcaaa agacgaccat tttatgaatg
    10201 atgaaaaatg gaattacatt aaagaaagat gggaaaaatt tagatttatt tacactttag
    10261 aagtatcact atttaggaat gaattaaaaa aagctgtaga atctggggat tttaattttg
    10321 aaatttatca tgatgataaa tctggagtca ctatgaatgg cgacggtata agctatggtt
    10381 catggggtca aaatgtagca ttaattaatg ttgcaaaagc atttggattc aatcccaaat
    10441 atatggaaga tgaatcacat gctaatattc tttctagaaa atataattta acatttttat
    10501 cttttcttaa aaaatatgta ccaggttttg aaaattcttt tatccttgat cagggaacta
    10561 gagctactaa cagatcatgt aggcacattg ataattcatc tatgatggac gaaattcttg
    10621 attttccaat gtatacattt aagactatgt atagctatca aactcctaaa gaaataacgt
    10681 acaaatcaat tgtaggtgga aaattgaata atttgtttgt aataggtaaa ggtgcttacc
    10741 aatccgaaag ttttagatca caaattagtt gtatgttaat ggggatatca tcagcagcag
    10801 cggcaaaaac tattattgaa gataaaagca atactctaaa cattaataga gatttgttta
    10861 aatcgaactt agatatatta tttacatggg agttttctaa ttggattact gattaacaaa
    10921 taataaggga atattaaatg aatgatacta ataaaaatca atactttgtt tatgaatttg
    10981 atactagctt tgtagaaatg tcttattctc agttttttga aggatattgt attctttttt
    11041 caaaagaacc ctatccttct ttatctgata tgccaatgga atttagaaaa gaatttttaa
    11101 tggaaatgag tatattaggt gatgcgatgc ttgaagtgtt aaatgcctat agaattaatt
    11161 atgatatctt aggaaattca acacctgtac ttcatgctca tttatttccg agatacatgt
    11221 gggaagagga agagagaaga aaaactgtag ttcatagata tgattcttca gtttttagca
    11281 atcctgagtt tgaatttact atagaaaagc atggaccaat tattaaaaaa ctgaggtctg
    11341 ctattgatag acattataga gagtatcaac aattattaga tgattcaaac tgaactccac
    11401 attttcgatt agatttaata ccttgtatat tacaactagg ttgtggatga tataattttt
    11461 tgaaaaatat attttaggaa tgacttataa agcaattata tcagaggcta ttaaatcaat
    11521 ggccaaagct tatgctcctt attctgattt taaggttgga gctgcgttag taactgaaga
    11581 taaaaaattt tttctggatg taatatagaa aatgctgctt tttctcctac ttgctgtgca
    11641 gaacgagtat ctttttataa agctgtattt aaaggagaac gttcgttttc aagaacatgt
    11701 attgtaggtg gaaaaaatgg tatcttatca tcttttatag caccttgcgg agtgtgtcgc
    11761 caggtaatga tagaatttgt aatcctgatt catttgaagt tatattggct agtgatgttg
    11821 atacttataa agtatataaa cttaaagaac tactaccagt tagttttaat caagaaaatt
    11881 tataacagga gggaactatg gaattagttg aatttaaaaa tggacatgtt aatacggttc
    11941 atgagcatat cgcaataacg aaagtaaaag agtttctaaa taatcatgat atattaaaag
    12001 ataaaggtgg catacataaa atttctactg atttctttta caatataatt gatatggaaa
    12061 ctacaactga agagaataga ccgtgggaaa gtcataagga atattatgat gtccattatc
    12121 tgttaaatgg agaagaaaca attttatata acttcttgtc tcaaatggaa ttgtctgaat
    12181 ataaagtaga tgatgattgg caacaaatga atgggacagc tttatttagt attaaattaa
    12241 agaaagatat gttacttttg ttggaaccaa acgatgcaca tagaacaggc ctattggttg
    12301 aagaaccact taatattaag aaagttgttt ttaaggtaaa aatttaactg aaagtacttt
    12361 cggggattaa gaattttaaa atatttaaga aattgtattc taaagttcta aaattataga
    12421 tatttaattt aaagcttgtt aaaatagaat agtaacactc atctgctttc aaaaagcatt
    12481 ctattccatc tccgattaac gatggacttt atcacctcct tctccagtcc ttgtatgaca
    12541 tcttggagtt gattcatgac atcttccaaa gttcgaaagg ctttattctt aaatccacgt
    12601 ttatgaatct ctttccacac ttgttcgatg ggttcatctc tggtgtgtat ggaggaataa
    12661 atgcaaaacc aatattagtc ggagtcttta agg
//
LOCUS       DAVBCV010000057        12573 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBCV010000057 DAVBCV010000000
VERSION     DAVBCV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.057"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQ516_001701"
     CDS             complement(251..433)
                     /locus_tag="VQ516_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512102.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQ516_001702"
     CDS             complement(649..1881)
                     /locus_tag="VQ516_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2512103.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ516_001703"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ516_001703"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2512104.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQ516_001704"
     CDS             complement(3048..4217)
                     /locus_tag="VQ516_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2512105.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQ516_001705"
     CDS             complement(4403..5707)
                     /locus_tag="VQ516_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2512106.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQ516_001706"
     CDS             complement(5824..6978)
                     /locus_tag="VQ516_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2512107.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQ516_001707"
     CDS             complement(6975..7526)
                     /locus_tag="VQ516_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2512108.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQ516_001708"
     CDS             complement(7519..8655)
                     /locus_tag="VQ516_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2512109.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ516_001709"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ516_001709"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2512110.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGELFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ516_001710"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ516_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2512111.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYLVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQ516_001711"
     CDS             complement(10172..10903)
                     /locus_tag="VQ516_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2512112.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQ516_001712"
     CDS             complement(10905..12350)
                     /locus_tag="VQ516_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2512113.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4237 a         2433 c         1656 g         4247 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcgtccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttc ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactagat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaaa tatagtaaaa
    12481 tgaaataaga ataggacaaa tcaatcagga cagtcaaatc gatttctaac aatgttttag
    12541 aatcagatgt gtactattct agtttcaata taa
//
LOCUS       DAVBCV010000058        12421 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBCV010000058 DAVBCV010000000
VERSION     DAVBCV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.058"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            301..561
                     /locus_tag="VQ516_001713"
     CDS             301..561
                     /locus_tag="VQ516_001713"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2512114.1"
                     /translation="MSGGETKRVALAKIILKNPPLIFADELTASLDSTTSQGIMKLLL
                     GMKNKNRLIIIATHNPDIWNQADEVINLNQLLQSIYIFYILK"
     gene            complement(665..1135)
                     /locus_tag="VQ516_001714"
     CDS             complement(665..1135)
                     /locus_tag="VQ516_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2512115.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQTLLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(1152..3425)
                     /locus_tag="VQ516_001715"
     CDS             complement(1152..3425)
                     /locus_tag="VQ516_001715"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2512116.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLASDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3772..6873
                     /locus_tag="VQ516_001716"
     CDS             3772..6873
                     /locus_tag="VQ516_001716"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2512117.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASFLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNEIVMKT
                     IYR"
     gene            6955..7962
                     /gene="pfkA"
                     /locus_tag="VQ516_001717"
     CDS             6955..7962
                     /gene="pfkA"
                     /locus_tag="VQ516_001717"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2512118.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            8019..9524
                     /gene="pyk"
                     /locus_tag="VQ516_001718"
     CDS             8019..9524
                     /gene="pyk"
                     /locus_tag="VQ516_001718"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2512119.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(10256..10481)
                     /locus_tag="VQ516_001719"
                     /pseudo
     CDS             complement(10256..10481)
                     /locus_tag="VQ516_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11329..12201
                     /locus_tag="VQ516_001720"
     CDS             11329..12201
                     /locus_tag="VQ516_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2512120.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            12214..12378
                     /locus_tag="VQ516_001721"
     CDS             12214..12378
                     /locus_tag="VQ516_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512121.1"
                     /translation="MTVILGFLFLFLMTWWVISIVNSQKPRKNETFIGYIQRYDIDGN
                     GYQSLPCPQN"
BASE COUNT         3792 a         2156 c         2655 g         3818 t
ORIGIN      
        1 gagttgtttt gtcgcttttc attataggtc atatgggact ttttttctac aataaaatag
       61 gctccataat atctatagtg gatttaccca ctacaaatat tatagaaccc ttttttattg
      121 acagtatcag gatatctttt tcaaattttt ggtttgttag ataatgaaaa tgtttctatc
      181 aatctagatt taggattaat aaatcgtaaa tgtaattata aagaaaaaga taagttaaaa
      241 aaagatgtat tagcaagagt tggtctaact catattcatc tataacaaaa gatatatgaa
      301 ttgtctggcg gtgaaacaaa acgtgttgct ttagcgaaaa tcattcttaa aaatccacca
      361 ttaatattcg cggatgagtt gactgcatca ctcgattcga cgacttcaca aggaattatg
      421 aaattgttat taggtatgaa aaataaaaat cgtctaatca tcattgcaac tcataatccc
      481 gatatttgga atcaagcaga tgaagttata aatttaaatc aattattaca gtcaatatat
      541 atcttctata ttttaaagta attttatctg aatccgttaa gagactattg tcccaggctc
      601 tttctgatgt taaagacttt ctaccaggtt ttttaaaagc ataattgtta gttgtagtca
      661 tttattattc ttcaaagaaa aatggtgggg cgaatttttt cagttcttca aagcactttt
      721 gagcagtatc tgcatcttca cagatgataa gacagacatc attaccacaa agggtagcga
      781 tagcgtcagg gaagctcaaa gtatcaatga tagaaccaaa ggattgagcc agtccaggaa
      841 gggtttttag taggacttgg tgttgaactg ggcgcatcca gacaagggcg tcttccatgt
      901 agagttcgag acgtttttcc cattttgaga tggaaccatt gttaagaaca taataagcgc
      961 tatcttcttc gcggactttt gataggttca tatttttgat gtcgcgtgag agggttgcct
     1021 gagttacttg aatgtcgttc tcagcaagaa gggtttgcaa ctcagcctgt gtatgaatct
     1081 tgttttttgt gataagagcg cgtataagtt ggtggcggtg ttctgattta ttcataataa
     1141 tgtaactcct tttagcaagg taaggtaagc atggactgag cgaggtcgac agtcaagtgg
     1201 tagtctgtat tgtcacggat ggtgatttca aagtcagtag tatagaggac taaacggaga
     1261 gtgtctcctt cttttagctt gtaaatagtt ggctgcagtt caaattgaac gtccatccat
     1321 tcatctgcag taatatcctc tactaacagt aaatcatttc tattttgtaa attaaggtaa
     1381 ccttttgtca cgactctttg tgcctctggt ctaaatggca attcacagag attttccaac
     1441 atgtgatagc gaccgttgtc aatggttcta gcacttaaaa tagctggata aggttgtagg
     1501 tatttctttt gcccaaattc tagcagttgg gcagataaga gccccttgtt tgtactggat
     1561 ttgatacgaa gattgagctg agcgcgaccg tttaggtgga gatctttagt cacaggaagg
     1621 ttaatagtaa tctgattagc tttcccttga tagagctctg tattgaaggt ttggtatgtc
     1681 ttaccatagc gctcaaaatc cttatctggg tactggtttt gaatagcttg ctcttcttga
     1741 ccaagtgaga aggtttcaca gttttcttgc ccaccgaagt tatcaagtga taaccaagtc
     1801 tgtggagctg tattgtcctg ccagataaca gtaggaagtt gaaagtctgt ttcctgtcct
     1861 agtaatttct tggtcaataa ggcatttatg gactcacgga agtcaattga ttgccaattg
     1921 ttcatgtaaa catgggcacc attatggaaa aagagatgct tgtgtatatg agtaggaaga
     1981 gcatggaaca tctggtaaac atgaagtggt ttgacattcc aatcctgaga accatgagta
     2041 aagacaacct ctgcctttac tttatgggca ttgagcagat aattgcggtc atgccaaaac
     2101 tgattgtagt ccccagtttt tcggtctagt tgagctttca ctttttctaa gtcagcttgg
     2161 tgagcttcat tgccacggat atagtcgcta gccaagagat tacgagaata ggttaactca
     2221 gcaagggagt caaagtcctc acctggataa ccacctgggc tagtcaccag accgttttca
     2281 cggtagtagt tgtaccatga tgaaattcct gcctcggcaa tgataacttc taaaccatcg
     2341 actcctgtag tcgcaagacc attggacatg gtacctagat aggaaagtcc cgttgtagca
     2401 acttttccgt ttgaccaatc agccttgact tgacgctggc gcgtgtgatc agtaaaggca
     2461 cggcaacggc cgttaagcca atcgatgaca tttttataag cctcgatttg ctggtagtct
     2521 ccattagtca tgaaacctgt cgagtctttg gtaccaacac ctgagacata gagattggca
     2581 aagcctctcg gaaggaagta gtcgtttagt gtatagctag agttgatgtg agttagcttt
     2641 tcctcagcct ctgctataag ctcagcttta ccttggggtt ggacgagatt tagttgaggt
     2701 ttctctagct caatcttgtg aggaagttta acctcaagct cgccctccat cttgtagaga
     2761 gccttgtcac tagccttgtc attggttccc tgatgataag ggctggctgt catgatggca
     2821 gggatttttc catcaaaacg agggcgaata atgctaacct ttactaggtc tgatagccct
     2881 ttttggtcag tatcgacacg agactcaacg taaacgactt cacgaatgac atcctggtta
     2941 gaaaaagtag ccaaactctt gccgttaaag tagtggtagt cattatcctc cggaataaga
     3001 ccatcactaa caagttggtc gataagagta tttccttttt tggtgcgagt attgagtaac
     3061 tgatagagat tttcaatcaa gtcaccatat ataatgggaa atccagtttc tttacgaaaa
     3121 acgtcactat cttcgaagtc aaccaaataa gaaaagccta aaagttgaaa agcaacagta
     3181 taaaaaatat ctgctgtcag ttcatcttct gattgaaaaa atgtcagcag gtctgttttt
     3241 ttatcagctg ctaggataga aagtgggtag ttggtgtctt gataagtgaa aaagaaacga
     3301 cgtaaaaagg tttcaagtga gtctttgtga ttggctgtat tttgtaaatc aaagccacat
     3361 ttttttagtt cagataagac attttctttt ggaaaattga tataactata ttgattaaaa
     3421 cgcatagaac ctccatatag aatgacagtt aaggttatta tatcaaaaaa aaagcagaaa
     3481 gggaattgtt aacttcaaaa ggaaataatc caataaaaat gaataaagta ctaaattcaa
     3541 tatagagaac agagtaacaa taagaataaa tagatagggt ataaaagttc taggagattt
     3601 atattatatg ctttctgttt ttatatacaa tatagtataa atataaaaat gatgacaaaa
     3661 atacaaatga atagaaaata aattagtaag ctgatgaaat ttttctcaag agaagccatt
     3721 tataggtgaa aatggtataa tatagtgaga aggatagagg agaagtgtaa attgatcgca
     3781 caactagata caaaaacagt ctatagtttt atggaaagcg tcatttcgat cgaaaagtat
     3841 gtgagagcag ctaaagaata cggctacact catttggcta tgatggatat tgacaatctt
     3901 tatggcgctt tcgactttct agagattaca aaaaaatacg gcattcatcc tttgctaggg
     3961 cttgaaatga cagtgtttgt agatgatcag ggagtgaatt tgcgcttttt agctctatct
     4021 agtgtgggct atcagcagtt gatgaagctt tcgacagcca agatgcaggg ggagaaaact
     4081 tggtcagtcc tgtcccagta cctggaggat atcgcggtca ttgtgcctta ttttgataga
     4141 gttgagtcgt tagaactagg ctgtgattac tatatagggg tttatccaga aacactagca
     4201 agcgaatttc atcatcctat cttacctctt tatcgggtca acgcttttga aagcagggat
     4261 agagaagttc ttcaagtttt aacagcgatt aaagaaaatc taccgctcag agaagttccc
     4321 ttgcgttcga gacaagatgt ctttatatca gcaagttttt tagagaaact attccaagag
     4381 cgttttccgc aagctttgga caatttagaa aagcttattt caggcatttc ttacgacttg
     4441 gatactagtc tgaaactgcc tcgttttaat ccagctagac cagcagtaga ggagttgaga
     4501 gagcgtgctg aactggggct tgttcagaag gggttgacta gtaaagaata tcaagataga
     4561 ctagaccaag aattgtctgt tattcatgat atgggctttg atgattattt cttggttgtt
     4621 tgggatttgt tgcgttttgg acgatcgaat ggctattata tgggaatggg aaggggttct
     4681 gcagtaggca gtttggtttc ttatgcctta gacatcacgg ggattgaccc agtagagaaa
     4741 aatctgattt ttgaacgctt tcttaatcgt gaacgctata ccatgcctga tattgatatt
     4801 gatatcccag atatttatcg tccagatttt atcagatatg ttggtaataa atatggtagt
     4861 aaacatgcgg cacaaatcgt tactttttca acctttggag ccaagcaagc tcttcgagat
     4921 gtcttgaaac gctttggtgt gccagagtat gaattatctg caattactaa gaaaatcagt
     4981 tttcgtgaca atcttaagtc ggcctatgag ggcaatctcc agtttcgtca gcaaatcaat
     5041 agtaagttag aataccaaaa agcttttgag attgcttgca agatagaggg ctatccaagg
     5101 caaacctctg tccatgcggc tggtgttgta attagtgacc aagatttaac caactacatt
     5161 cctctaaagt atggtgatga aattccactg actcagtatg atgctcatgg agttgaggct
     5221 agcggacttt tgaagatgga ctttctggga ctacgaaatt tgacctttgt ccagaagatg
     5281 caagagttgc ttgctgaaac agaaggtatt catctgaaaa ttgaagaaat cgatttagaa
     5341 gacaaagaaa cattagcttt atttgcctct ggtaatacaa aaggtatctt tcaatttgag
     5401 caaccaggtg ccattcgtct gcttaagcgt gtgcaaccag tctgttttga agatgtcgtc
     5461 gcgactactt ctctaaatcg accgggtgct agtgactata tcaataattt tgtggcaaga
     5521 aagcatgggc aggaagaagt gactgttctg gatccagtac tggaggatat tttggctcca
     5581 acctacggca taatgctcta tcaggagcag gttatgcagg ttgcccagcg acttgccgga
     5641 tttagtcttg ggaaagccga tattttgcgt cgggctatgg ggaaaaagga tgcctctgcc
     5701 atgcatgaga tgagggcttc ctttattcaa ggttcattag aagctggtca tactgtggaa
     5761 aaagcagagc aggtctttga tgttatggag aagtttgcag gttatggttt taacaggtca
     5821 cacgcctatg cctactcagc cttggccttc cagttggctt atttcaaaac gcattatcca
     5881 gccatttttt atcaggtcat gttaaattct tccaacagtg attacttaat agatgcactt
     5941 gaagcaggtt ttgaagtagc ctctctatcc atcaacacca ttccctatca cgataaaatt
     6001 gccaacaagg ccatctatct aggtttgaaa tccattaaag gagtcagtaa tgatttagct
     6061 ctctggatta ttgaaaatag accttattct aacattgaag attttatagc taaattacct
     6121 gagaattatc tgaaacttcc tctgctagaa cctttggtaa aagttggtct tttcgattca
     6181 tttgaaaaaa atcgtcaaaa agtatttaat aacttagcta atctatttga atttgtgaaa
     6241 gagttgggaa gtttgtttgg agatgctatt tatagttggc aggaatcgga agattggacg
     6301 gaacaagaaa aattttatat ggaacaagag cttttaggga taggtgtcag caaacatcca
     6361 ctacaagcta ttgcaagtaa ggctatttac ccgattaccc caatcggaaa tttgtcagaa
     6421 aatagctatg ctattatctt ggttgaagtt cagaaaataa aagtgattcg taccaaaaag
     6481 ggtgaaaata tggccttctt acaggcagat gatagtaaga aaaaattgga tgttacactc
     6541 ttctcagatt tatatcgaca ggttggtcag gaagtaagag aaggagcatt ctactatatc
     6601 aaagggaaag tccaatcacg tgacggtcgt ctgcaaatga ttgcacaaga aataagagaa
     6661 gcagttgctg aacgcttttg gatacaggtg aaaaatcatg aatcggatca agaaatttca
     6721 cgtattttag aacaatttaa aggcccaatc ccagtcatca tccggtatga agaggaacag
     6781 aaaaccatcg tttctcccca tcattttgta gctaaatctg atgaattaga ggcgaaattg
     6841 aatgaaatcg ttatgaaaac gatttatcgc taaaaatacg gaaaatagaa gaattttaaa
     6901 atcaaatgtg atataatcaa gaagaatgtt aaaagaaaaa ggagcataac caatatgaaa
     6961 cgtattgctg ttttgactag tggtggagac gcccctggta tgaacgctgc catccgtgca
     7021 gttgttcgtc aagcaatttc agaaggaatg gaagtgtttg gtatctatga cggatatgct
     7081 ggtatggttg ccggtgaaat tcatccccta gatgcagctt cagtagggga catcatttct
     7141 cgtggtggta ctttccttca ctcagctcgt tacccagagt tcgctcaact tgaagggcaa
     7201 cttaaaggga ttgagcaatt gaaaaaacac ggaattgaag gtgtagttgt tatcggtggt
     7261 gacggatctt accacggcgc tatgcgtttg actgaacatg gcttcccagc tattggtctt
     7321 ccaggtacaa tcgataacga tatcgttggt actgacttta caatcggttt tgacacagcg
     7381 gttactactg ccatggacgc tatcgataag attcgtgata catcatcaag tcaccgtcgt
     7441 acttttgtaa tcgaagttat gggacgtaac gctggtgata tcgctctttg ggctggtatt
     7501 gcaactggtg ctgatgaaat catcatccct gaagcaggct tcaagatgga agatatcgta
     7561 gcaagcatca aagctggtta tgaatgtggt aaaaaacaca atatcatcgt cttagctgaa
     7621 ggtgtgatgt cagcggctga atttggtcaa aaacttaaag aagctggaga tacaagcgac
     7681 cttcgtgtaa cagaacttgg acatattcaa cgtggtggtt ctccaactgc gcgtgaccgt
     7741 gttttggcgt cacgtatggg tgcacatgct gttaaacttc ttaaagaagg tatcggtggt
     7801 gttgcggttg gtattcgtaa cgaaaaaatg gttgaaaatc caattcttgg tactgcagaa
     7861 gaaggggcat tgtttagcct tactgcagaa ggtaagattg tggttaacaa cccgcacaaa
     7921 gccgatattg agctatctag cttgaataag agcttgtcat aatttacaat ttagttaata
     7981 agaccaaaaa ggtcatatat aaaggagtca caaaaatcat gaacaaacgt gtaaagatcg
     8041 ttgcaacttt gggtcctgcg gtagaaatcc gtggtggtaa aaaattcggt gaggacggat
     8101 actggggtga aaaacttgat gttgaagctt cagctaaaaa cattgctaaa ttgattgaag
     8161 ctggtgctaa cacattccga ttcaacttct cacacggcga ccaccaagaa caaggtgagc
     8221 gtatggcaac tgttaaactt gcggaaaaaa ttgcaggtaa aaaagttggt ttccttcttg
     8281 atacaaaagg acctgaaatc cgtacagaat tgttcgaagg tgaagctaaa gaatattcat
     8341 acaaaactgg tgaaaaaatt cgtgttgcaa ctaaacaagg aatcaaatca actcgtgaag
     8401 tgattgcgtt gaacgttgct ggtgctcttg atatctatga tgatgttgaa gttggtcgtc
     8461 aagttttggt tgacgatggt aaacttggtc ttcgtgtggt tgctaaagat gatgcaactc
     8521 gtgaatttga agttgaagtt gaaaacgatg gtatcatcgc taaacaaaaa ggtgtgaaca
     8581 tccctaacac taaaattcct ttcccagctc ttgctgaacg cgataacgac gatatccgtt
     8641 tcggtcttga acaaggtatc aacttcatcg caatttcatt cgtacgtact gcaaaagatg
     8701 tgaacgaagt tcgtgcaatc tgtgaagaaa ctggaaacgg acatgttcaa ttattcgcta
     8761 aaatcgaaaa ccaacaaggt atcgataact tagacgaaat catcgaagca gctgatggta
     8821 ttatgattgc tcgtggtgat atgggtatcg aagtaccgtt cgaaatggtt ccagtttatc
     8881 aaaaaatgat tatcaagaaa gtcaatgctg caggtaaagt tgttatcact gcaacaaaca
     8941 tgcttgaaac aatgactgaa aaaccacgtg caactcgttc agaagtatca gacgtattca
     9001 acgctgttat cgacggaact gacgctacaa tgctgtcagg cgagtctgca aacggtaaat
     9061 acccactcga gtcagtaact acaatggcta caatcgacaa gaatgctcaa gctcttctta
     9121 atgaatacgg acgtcttgat tcagattcat ttgagcgtaa ctctaagaca gaagtaatgg
     9181 cttctgctgt taaagatgct actagctcaa tggatatcaa attggtcgta actcttacta
     9241 agacaggtca tactgcacgt ttgatttcta aataccgtcc aaatgctgac atcttagcat
     9301 tgacatttga cgaattgaca gaacgtggct tgatgttgaa ctggggtgtt atcccaatgt
     9361 tgacagatgc tccatcttca actgacgata tgttcgaaat cgctgaacgt aaagcggtag
     9421 aagcaggtct cgttgagtca ggcgatgata tcgttatcgt tgctggtgtg ccagtaggag
     9481 aagctgttcg cacaaacaca atgcgtatcc gcacagtacg ttaagaaaaa tataaaaacc
     9541 tatcatatcc agctttagag cttgtgtgat aggctttttg tatagagggt aagaaatagg
     9601 caaaactttc ataatggatt gatactcttc gaaaatcaaa ttcaaaccac gtcagcgtcg
     9661 ccttaccgta tatatgttac tgacttcgtc agttctatct acaacctcaa agcagtgctt
     9721 tgagcaacct gcggctagct tcctagtttg ctctttgatt ttcattgagt atgaaataag
     9781 atatgcacaa attgattagg aaattcaaat gaatttctac aaatgtttta gcaatcgtaa
     9841 tgtacttgtc tagattcgat ctgatatatt ttcgatttaa tgatatggta tttaaaacct
     9901 ccaaagtagc ttgctccatt cttttactta cgtgagtgta gatgttattt actgttttag
     9961 cgtttttgtg ttccactcta accattatag cattcttctc agctagtgta ctaaggagtg
    10021 tgtgcctgaa aatatgggaa ctaaggggct ggtttatcgg tttctctagt ttagtatttg
    10081 ccttttgcaa agtgatgtta aatgcctttc tctaaattta catatcacta ttgtttaaca
    10141 aaatctaatc tactttaggt cacttattct ttttttgaaa tgtagaatga actttttcaa
    10201 agtttttcga atcttttaaa atctgtttgt tttatatcgc cattctcccc cttttttaat
    10261 tctccctata tagcctgaca actttcccga tggtacgaat atggttgctt tcgtctaggt
    10321 ggatgtcggg gtatttggga ttgagttttt ttaaggtagc cttggcggag tttcttgaca
    10381 tagttagcgc cgtctacttg gaagatgccg atggtattat agtcaatctg tggggtattc
    10441 ttgataaata ggtagtcgct gtttcttatc tttggctcca tggacttgct gacgacataa
    10501 gcgattgggt cgtagtcgtc tgggataatg gaaactccat aactaaatcg tcgtcctgca
    10561 tcgagcggct acctgcagag ataaactacc taacacgaga gtaagtagtc tgtctgtagt
    10621 cgtccagtct gatgattttt acgatatttc gtttttctgc tcatacagtt gcctctcggc
    10681 ataggtcaga actttacctt gtctgggtgg tgcccgttgg tcgtagatag attggatatc
    10741 gctaggagaa tccttttgaa ctggaggaaa gagggtatca atcaagctac tgaatacttt
    10801 agctaagtca aatatagtat ttttcttagt agacctaacc cttttttcat aatttccaat
    10861 ggtgttttta cttataccaa tcttagtacc caattcttat tgagtccaac cattactagt
    10921 ctatattgtt ttatagttga ttgagtttgg aatagtacgc tgtagctgct aaaacatttc
    10981 taggaattaa tttgactttc ctaatagagt tgttcatatc ttatttcaat ctactatgtt
    11041 tttcacctct aacaatcgca atctcttctt tatccatgaa tgaaatcgct ttctattttt
    11101 gtaagtaaag catcacacga aatccacgaa aatgaaaacc tttgttgtgt tttcgtaaaa
    11161 aatttgttga cagagcacga aacgcctact ataattaaat caagcttaag gaaataacaa
    11221 aaaaacaaat cggagggaaa gacgatgaac acattaaatg agaaagtaat caatatctgt
    11281 aaagcagtag ttaaagaaac tttaatccaa gacatttagg agaaagaaat gaagaaatca
    11341 agaaaattag ccacattagg aatctgttca gccttgtttt taggcttggc tgcttgtcaa
    11401 caacaacatg ctacttctga ggggacgaat caaaggcaaa gcagttcagc gaaagttcca
    11461 tggaaagctt catacaccaa cctaaacaac caggtaagta cagaagaggt caaatctctc
    11521 ttatcagctc acttggatcc aaatagtgtt gatgcatttt ttaatctcgt taatgactat
    11581 aataccattg tcggctcaac tggcttatca ggagatttca cttcctttac tcacaccgaa
    11641 tacgatgttg agaaaatcag tcatctctgg aatcaaaaga agggcgattt cgttgggacc
    11701 aactgccgta tcaatagtta ttgtcttttg aaaaattcag tcaccattcc aaagcttgaa
    11761 aagaatgacc aattgctttt cctagataac gatgcgattg ataaaggaaa ggtctttgat
    11821 tcacaagata aggaagagtt tgatattcta ttttcgagag ttccaactga ggcaactaca
    11881 gatgtcaagg ttcacgctga aaagatggaa acattcttct cacaatttca attcaatgaa
    11941 aaagctcgaa tgctgtctgt agtcttgcac gacaatctgg atggcgagta tctgtttgta
    12001 ggccatgttg gggtcttagt acctgctgat gacggtttct tatttgtaga gaaattgact
    12061 ttcgaagagc cctaccaagc gattaaattt gctagtaagg aagattgcta caagtatttg
    12121 ggcaccaagt atgcggatta tacaggcgag ggactggcta agccttttat catggataat
    12181 gataagtggg ttaaacttta atagtgttat gatatgacag taatcttagg atttttattt
    12241 ctatttttga tgacttggtg ggttatttca atcgttaata gccagaaacc acgcaaaaat
    12301 gaaacattta ttggctatat ccaaagatac gatattgatg ggaatggcta tcaatcatta
    12361 ccgtgtcctc agaactgaga ggagttcttg aaaccgtaaa accgctttga acaagagtta
    12421 c
//
LOCUS       DAVBCV010000059        12019 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBCV010000059 DAVBCV010000000
VERSION     DAVBCV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.059"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..165
                     /locus_tag="VQ516_001722"
     CDS             <1..165
                     /locus_tag="VQ516_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512122.1"
                     /translation="AYNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLI
                     AGIINHELGF"
     gene            357..2648
                     /gene="pcrA"
                     /locus_tag="VQ516_001723"
     CDS             357..2648
                     /gene="pcrA"
                     /locus_tag="VQ516_001723"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2512123.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2691..3371
                     /gene="radC"
                     /locus_tag="VQ516_001724"
     CDS             2691..3371
                     /gene="radC"
                     /locus_tag="VQ516_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2512124.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3368..4057)
                     /locus_tag="VQ516_001725"
     CDS             complement(3368..4057)
                     /locus_tag="VQ516_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2512125.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4075..4716)
                     /locus_tag="VQ516_001726"
     CDS             complement(4075..4716)
                     /locus_tag="VQ516_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2512126.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4799..5014)
                     /locus_tag="VQ516_001727"
     CDS             complement(4799..5014)
                     /locus_tag="VQ516_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2512127.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(5022..5369)
                     /locus_tag="VQ516_001728"
     CDS             complement(5022..5369)
                     /locus_tag="VQ516_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2512128.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5374..6489)
                     /locus_tag="VQ516_001729"
     CDS             complement(5374..6489)
                     /locus_tag="VQ516_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2512129.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6499..7458)
                     /locus_tag="VQ516_001730"
     CDS             complement(6499..7458)
                     /locus_tag="VQ516_001730"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2512130.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7571..8140)
                     /locus_tag="VQ516_001731"
     CDS             complement(7571..8140)
                     /locus_tag="VQ516_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2512131.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8268..8939
                     /locus_tag="VQ516_001732"
     CDS             8268..8939
                     /locus_tag="VQ516_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2512132.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8923..9741
                     /locus_tag="VQ516_001733"
     CDS             8923..9741
                     /locus_tag="VQ516_001733"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2512133.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9738..10634
                     /locus_tag="VQ516_001734"
     CDS             9738..10634
                     /locus_tag="VQ516_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2512134.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10678..11652
                     /gene="pta"
                     /locus_tag="VQ516_001735"
     CDS             10678..11652
                     /gene="pta"
                     /locus_tag="VQ516_001735"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2512135.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3502 a         2300 c         2586 g         3631 t
ORIGIN      
        1 gcctataacc atgcgctatc caaggagaga agcaaggttg agaacatctt tgccaaagta
       61 aaaacgttta aaatgttttc aacaacctat cgaaatcatc gtaaacgctt cggattacga
      121 atgaatttga ttgctggcat tatcaatcat gaactaggat tctagttttg caggaagtct
      181 attgaggtat tgagctagtt tatgaaaaaa ttgggtgaaa aagtcgagtg ttttagaaac
      241 ccacagtgta gtattctagt ttcaatccac tatattttgc tactccccgt aaagtttcta
      301 ttttccctga tttctgatat aatagaaata ttgacttcaa gagtaaggaa gagaagatga
      361 acgcattatt aaatggaatg aatgaccgtc aggctgaggc ggtgcaaacg acagaaggtc
      421 ccttgctaat catggcagga gctggttctg gaaagactcg tgttttaacc caccgtatcg
      481 cttatttgat tgatgaaaag ctggtcaatc cttggaatat cttggccatt acctttacca
      541 acaaggctgc gcgtgagatg aaagagcgtg cttatagcct caatccagct actcaggact
      601 gtctgattgc gaccttccac tccatgtgtg tgcgtatttt gcgtcgcgat gcggaccata
      661 ttggctacaa tcgtaatttt acaattgtgg atcctggtga acagcgaacg ctcatgaaac
      721 gtattctcaa acagttgaac ttggatccta aaaaatggaa tgaacgaact attttgggga
      781 ccatttccaa tgctaagaat gatttgattg atgatgttgc ttatgctgct caagctggcg
      841 atatgtatac gcaaattgtg gcccagtgtt atacaaccta tcaaaaagaa cttcgtcagt
      901 ctgaatccgt tgactttgat gatttgatta tgctgacctt gcgtctcttt gatcaaaatc
      961 ctgatgtttt gacctactac cagcaaaaat tccaatacat ccacgttgat gagtaccaag
     1021 ataccaacca cgctcagtac caattggtca aactcttggc ttcccgtttt aaaaatatct
     1081 gtgtggttgg ggatgcggac cagtctatct acggttggcg tggtgctgat atgcagaata
     1141 tcttggactt tgaaaaggat taccccaaag ctaaggttgt tttgttggag gaaaattacc
     1201 gctcaaccaa aaccattctc caagcggcca acgaggttat taaaaataat aaaaatcgcc
     1261 gtcctaaaaa tctctggact caaaacgctg atggggagca aatcgtttac tatcgtgctg
     1321 atgatgagct ggatgaggct gtatttgtag ccagaaccat cgatgaactt agtcgcagtc
     1381 aaaacttcct tcataaggat tttgcagttc tctatcggac taatgcccag tcccgtacaa
     1441 ttgaggaagc cctgctcaag tctaacattc cttataccat ggttggcgga accaaattct
     1501 acagccgtaa ggaaattcgc gatattattg cttatctcaa ccttattgct aatttgagtg
     1561 acaacattag ttttgagcgt attatcaacg agcctaaacg tggaattggt ctaggtacag
     1621 ttgagaaaat ccgtgatttt gcaaatttgc aaaatatgtc tatgctggat gcttctgcta
     1681 atattatgtt gtctggtatc aagggtaagg cagcccaatc tatctgggat tttgccaata
     1741 tgatgcttga tttgcgggag cagctagacc acttaagcat tacagagttg gttgagtccg
     1801 tcctagaaaa aacaggttat gtcgatattc ttaacgccca agcgactcta gaaagcaagg
     1861 caagggttga aaatatcgaa gagtttcttt ctgttacgaa gaactttgat gacaccactg
     1921 atgtgacaga agaggaaact ggtctggaca aactgagtcg tttcttaaat gacttggctt
     1981 tgattgccga cacagattca ggtagtcagg agacatcaga agtgaccttg atgaccctgc
     2041 atgctgccaa aggtctcgaa tttccagttg tctttttgat tgggatggaa gaaaatgtct
     2101 ttccacttag tcgtgcgact gaagatccag atgaattaga agaagagcgc cgtctagcct
     2161 atgtaggtat cacgcgtgca gagaaaattc tctatctgac caatgccaac tcacgcttgc
     2221 tttttggtcg taccaattat aaccgtccga ctcgttttat taacgaaatc agttcagact
     2281 tgcttgagta tcaaggtctg gctcgtcctg caaatacaag ctttaaggca tcatatagca
     2341 gtggtagtat ttcctttggt caaggtatga gtttggctca ggctcttcaa gaccgtaaac
     2401 gcggtgctgc cccaaaatcg atccagtcaa gcggtcttcc atttggtcaa tttacagctg
     2461 gcgcaaaatc agcatctagc gaggcaaatt ggtccattgg tgacattgct ctccacaaga
     2521 aatggggaga gggaactgtt ctggaagttt caggtagcgg tgctaggcag gaattgaaaa
     2581 tcaatttccc agaagtaggt ttgaaaaaac ttttagccag tgtggctcca attgagaaaa
     2641 aaatctaatt ttccatcctt ctcacgaata ataaagtgag gaggattttt atgtacagta
     2701 tttcattcca agaagattca ctattaccaa gagaaaggct ggccaaggaa ggagttgaag
     2761 cgcttagtaa ccaagagttg ctagctattt tactcaggac aggaacacgt caagctagcg
     2821 tttttgaaat tgcccaaaaa gtcttgaaca atctttcaag cctaacggat ttgaaaaaaa
     2881 tgaccctgca ggaattgcag agtttgtctg gtattgggcg tgttaaggcc atagaattac
     2941 aagctatgat tgaactgggg catcgtattc acaaacacga gactcttgaa atggaaagta
     3001 ttctcagcag tcaaaagttg gccaagaaga tgcagcagga attaggggat aaaaaacaag
     3061 agcacctggt ggcactctat ctcaatactc aaaatcaaat catccatcag cagaccattt
     3121 ttatcggttc tgtaactcgt agtatcgctg aaccgcgaga gattcttcac tatgcaatca
     3181 agcatatggc gacttctctt atcttggtcc acaatcatcc ttcaggagcg gtagcgccta
     3241 gccaaaatga tgatcatgtc actaaacttg ttaaagaagc ctgcgaattg atggggattg
     3301 ttctcttgga ccatttgatt gtctctcatt ctaattactt tagttatcgt gaaaagacag
     3361 atttaatcta aagttcatta acgacatagt caaagagttt tttatctttg ggacgatttt
     3421 caaaaagaag ttctggatgc cattggacac cgagaaaggc gacatcatcc gtactcatga
     3481 cagcctcaat gataccatct ttaggatcat gagccacaac ttttaaattt ggtgctaagt
     3541 ccttgatgct ctggtggtgg aaggagttga tatgagagat ttctccatag atttcttgga
     3601 gaacggtatc tggttctgtt accaagcgtt gagttgtgta ctcaacagaa gaatcctgcc
     3661 aatggtcttc gatatcttgg tacaaagttc cacccatggc aacgttaaag agttgggtac
     3721 cacggcagac agagaaaatg ggctttttct gtttaatagc ttccttgatg agggccagtt
     3781 cgaagatatc tctttgaagg ttatagtcat cgctatcaat ggttttgggt tcgccataaa
     3841 attttggatc gacattttgc ccacctgtca agatgagctt gtcaatcaaa ctgatatagt
     3901 ggcaggccat ttcttgatca ccaatcggta ggatgatggg aatccctcca gcatctttaa
     3961 cgccttcaac aaagcctttt gctgcgtagc tcatcatgat gtcatcatct ggatgagctt
     4021 tttcgtttcc tgtaatccca ataactggtt ttttcataaa atgattttcg ctttctaatc
     4081 ctcttttcgc atgaagtaga ggagggtttg gagttcactt gtcaaatcga catactgaac
     4141 gaccacgtct tttggtaaat gcagatggac tggtgaaaaa ctgagaattc ctttcacacc
     4201 agcatcaacc aagagattag caacctcttg tgacttgacg ctgggaacag ttaggatagc
     4261 agtcttcaca tcagcatcct tgattttatc cttgatctga gaaatcccgt aaatgggaat
     4321 cccgtcagga gtttgggtac cgacttcagg atggtcgtct aggtcaaagg ccatgataat
     4381 cttcatcttg ttacgttcgt ggaagcggta gtggagaagg gcatggccca tatttccaat
     4441 accaaccagc atgacattgg taatagagtt gtcattgagc aaatcggcaa aaaatgtcat
     4501 tagttttttg acatcatagc caaaaccacg acgaccaagt tcaccaaaat aggaaaaatc
     4561 acgacgtacg gtcgctgaat caataccgat agcctctgca atttgcttag agttggcacg
     4621 ttcaatcttt tctgcatgaa atctcttaaa aattcgatag tagagagaga gtctttttgc
     4681 tgtagctttt ggaatagcaa actgtttatc tttcacaaaa tcacaacctt tctattcttc
     4741 tattttatag aaacattgtg aaaaaatcaa caaaaataag aaaaaactaa gaaaaatctt
     4801 agttttgatg taaaaaatct gcatgagata gaaaacggta gaggtctccg accagaccct
     4861 gataaacttt ttggccccta aaagtcagag aagtcacata aagtgtatct ggtaaggtta
     4921 cacatcctga caaagtcaac atgagagcct catgatcctc atacttgaga gtacgctcta
     4981 catgatagca gtccttatag gtcagttcaa acattttggc tctatctttc cgattttgta
     5041 aagacaccac gttctaccaa gctatccatg aggaagtaga atttttcctg atgaatatgg
     5101 tggtcttctg atttgaaaat atcaactaga cgaaggctaa acttgtcagt gatattgatt
     5161 ttagcccctg taagttcctt gttaatgatg attttgagtt ggaagccttc accgctgttt
     5221 ggcacttttt ccaaaaggcg agtcagttca tagttaccaa ccttagtttc aaaaaaggtg
     5281 ttatctttga gggtgaattt tttaacagaa gggctaagag tgtaatcgta acgacaattt
     5341 tttaactgaa tgattttttc aaatgccata tggctaacct ccgataattt cttttaaggt
     5401 ttttgcgagg gtttgtaggt cttcaacggt attttgtggc gacaaactga tgcgaaggga
     5461 ttccttcaag cgttctgaat ttgcgccata catggcttca agaacatggc tggattggac
     5521 aacgcctgca gtacaggctg agccagtaga gattgaaatt ccagctaaat ctagccgaag
     5581 gagtaagagg tcatttttct gaccaggaaa tccaatattg agaacataag ggagatgatg
     5641 ttttcctcta ttcaagtaat actgaatgcc ctccagctct gccagaaagg cggtttctag
     5701 attttgtaca tgttgaaaat gttcttcttg tttttctagg tcttctttta gggctgcaac
     5761 catgcctacg atagcaggca gattttcagt tcctgcacgt tttttctgtt cctggtctcc
     5821 gccatgtaga taggaatcaa agtccatgct agatgcgtag agaaaaccga ttcccttagg
     5881 accatggaat ttgtgggcag aagcagtgag aaaatcaatg cccaattctt ctgaatgaat
     5941 tgggatttta ccaatagcct gaactgcatc aacatgatag gcagcagggt gttgcttgag
     6001 tatttggcca atttcagcga tgggcagtag gtttcctgtc tcattattgg caaacatggt
     6061 agaaaccaaa atcgtatcgt cacgtaaagc cttttgaatt tgctgggctg tgatttcttg
     6121 attttctggc tggataatgg ttgcttcaaa cccaaagtgt tgaaccaagt aatcaattgt
     6181 ttcaaggaca gcatggtgct cgatggcagt tgtgatgata tgttttcctt gttcttggtg
     6241 acgaagacag tagccaatga tggtagtatt attgccttca gtcccaccag aagtgaaaaa
     6301 gatatgttga ggttttgtcc ttagtaactg ggctagttcc tgacgggctt ctcgcaagag
     6361 tttgccagct tgacgaccat gaccatgaat actagaagga tttccgtggg tttcttgcat
     6421 aaccttggtc atagctgaaa tagcaactgc tgacatagga gtcgttgcag cattgtccaa
     6481 ataaatcaaa gaatcacctt atttcttttt attgtaggca aagagtgggc tgactggttt
     6541 tctttcgtga atacggacga tagcatcacc aattaactca ctagcagtga tgtagcatac
     6601 atttttagga gttttttctt ttgttgctac tgaatcagtc acaagaattt ctttaatatt
     6661 agtattgtca agaagctcag cagctccctc gacgaagaga ccgtggctag aaacagcata
     6721 aatttctgta gctccttcac gttcaacgat tttagaagct tcagagaagg tacgtcctgt
     6781 atttaaaata tcatcaatca agatagcttt cttaccttca acatcaccaa taatataacc
     6841 ttcgttacga gttgcatcgt cttgagggta gtcgataatg gcgataggag catcaagata
     6901 ttcagccagg ctacgcgcac gtttgacacc tgaattttta gggctaacga caacaacatc
     6961 tgaaccaagc aatcctttat cgcagtaatg ttttgcgaat aggggaacag tgaaaagatt
     7021 atccactgga atatcaaaga aaccttgaac ctgaacggca tgcaaatcaa gagtcaggat
     7081 acgatcaact ccagccttaa ccagcatatt ggcaactagt tttgctgtaa gtggctcacg
     7141 aggacaagca atgcggtctt gacgtgcata gccaaaatat ggaaggacaa cgttgatact
     7201 gtgggcactt gcacgcacac aagcatcgac catgattaac aattccatta ggtggttgtt
     7261 gacagggaaa cttgttgatt ggatgatgta aacatcataa ccacggacac tttcttcgat
     7321 atttacttgg atttctccgt ctgaaaattg acgtgatgat agttttccaa gtgggacacc
     7381 aacagcttgg gcaatttttt gtgcaatctc ttggttagag ttgagtgcga aaagtttcat
     7441 gttttttcta tctgacatta tagaccgtcc tctgtaaact ttataaatcc tagttatatt
     7501 taccttacat atatgaactg ggatttgtgt atttttatct tttctatttt accaaaaaat
     7561 ggagattatt tcagctattt ttcatacttt tgacaaatcg aaccaatttt gaaggagctt
     7621 tttggtagga aatctgattt ttctctaaaa attgtcggaa atcctgtttg ccttgctcat
     7681 gattttccac ttcaagctcc aattcgtaat ctgttatatc aaagtatcgg ctctgatcca
     7741 gtgccatgag accaatagct gttttcattt catagcgaag cgttgttaga caaccaagaa
     7801 cctgccagtt cttactttgg ataccatgtt tcgccaattc atccagtact agtccttgag
     7861 gaagttcttc cttactcaga tagtcctcag catcttttag ttgcaatttt tggttgtatt
     7921 ccatgtttcc aacactctgc gggactttga gtgtcaactc agcccagtct tcaaaggttc
     7981 gaatgcgcat agcgactttc ttttctcgca gttcaaaatc aggcgtgtcg atgtagtaat
     8041 ttgtttgaag aacaggagtg acacctgtga actggtcttt tagacgattg tattcatctt
     8101 ttttcaatag tgttttcaat tcaatttcta aatgtttcat ttttcttacc tttttttatc
     8161 gttgaaagcg gatttatggt ataataagca ttgtatttat tgtatatgaa tctggagaaa
     8221 aaatcaaaga tatttttgac ggataatatg agaacaaggg agaatatatg accttagaat
     8281 gggaagaatt tctagatcct tacattcaag ctgttggtga gttaaagatt aaacttcgtg
     8341 gtattcgtaa gcaatatcgt aagcaaaata agcattctcc aattgagttt gtgaccggtc
     8401 gagtcaagcc aattgagagc atcaaagaaa aaatggctcg tcgtggcatt acttatgcga
     8461 ccttggaaca cgatttgcag gatattgctg gcttacgtgt gatggttcag tttgtagatg
     8521 acgtcaagga agtagtggat attttgcaca agcgtcagga tatgcgaatc atacaggagc
     8581 gagattacat tactcataga aaagcatcag gctatcgttc ctatcatgtg gtagtagaat
     8641 atacggttga taccatcaat ggagctaaga ctattttggc agaaattcaa attcgtactt
     8701 tggccatgaa tttctgggca acgatagaac attctctcaa ctacaagtac caaggggatt
     8761 tcccagatga gattaagaag cgactggaaa ttacagctag aatcgcccat cagttggatg
     8821 aagaaatggg tgaaattcgt gatgatatcc aagaagccca ggcacttttt gatcctttga
     8881 gtagaaaatt aaatgacggt gtaggaaaca gtgacgatac agatgaagaa tacaggtaaa
     8941 cgaattgatc tgatagccaa tagaaaaccg cagagtcaaa gggttttgta tgaattgcga
     9001 gatcgtttga agagaaatca gtttatactc aatgatacca atccggatat tgtcatttcc
     9061 attggcgggg atggtatgct cttgtcggcc tttcataagt acgaaaatca gcttgacaag
     9121 gtccgcttta tcggtcttca tactggacat ttgggcttct atacagatta tcgtgatttt
     9181 gagttggaca agctagtgac taatttgcag ctagatactg gggcaagggt ttcttaccct
     9241 gttctgaatg tgaaggtctt tcttgaaaat ggtgaagtta agattttcag agcactcaac
     9301 gaagccagca tccgcaggtc tgatcgaacc atggtggcag atattgtaat aaatggtgtt
     9361 ccctttgaac gttttcgtgg agacgggcta acagtttcga caccgactgg tagtactgcc
     9421 tataacaagt ctcttggcgg tgctgtttta caccctacca ttgaagcttt gcaattaacg
     9481 gaaattgcca gccttaataa tcgtgtttat cgaacactgg gctcttccat tattgtgcct
     9541 aagaaggata agattgaact tattccaaca agaaacgatt atcatactat ttcggttgac
     9601 aatagcgttt attctttccg taatattgag cgtattgagt atcaaatcga ccatcataag
     9661 attcactttg tcgcgactcc tagccatacc agtttctgga accgtgttaa ggatgccttt
     9721 atcggtgagg tggatgaatg aggtttgaat ttatcgcaga tgaacatgtc aaggttaaga
     9781 cctttttaaa aaagcacgag gtttctaagg gattgctggc taagattaag tttcgaggtg
     9841 gagctattct ggtcaataat caaccgcaaa atgcaacgta tctattggac gttggagact
     9901 acgttaccat tgacattccc gctgaggaag gctttgaaac cttggaggct attgagcatc
     9961 cattagatat tctctatgag gatgaccact ttctagtctt gaataaaccc tatggagtgg
    10021 cttctattcc tagtgtcaat cactctaaca ccattgccaa ttttatcaag ggttactatg
    10081 tcaagcaaaa ttatgaaaat cagcaggttc acattgttac cagactagat agggacactt
    10141 ctggcttgat gctctttgcc aagcacggtt atgcccatgc acgattagac aagcagttgc
    10201 agaagaaatc tatcgagaaa cgctactttg ctttggttaa gggagatgga catttggagc
    10261 cagaagggga aattattgct ccgattgcgc gtgatgaaga ttccattatt accagacgag
    10321 tggctaaagg cggaaagtat gcccatactt catacaagat tgtagcttct tatggaaata
    10381 ttcacttggt ctatattcac ctgcacactg gtcgaaccca tcaaatccga gtccattttt
    10441 ctcatatcgg ttttcctttg ctgggagatg atttgtatgg tggtagtctg gaagatggta
    10501 ttcaacgtca ggctctgcat tgccattacc tatcctttta tcatccattt ttagagcaag
    10561 acttgcagtt agaaagtccc ttgccggatg atttcagtaa ccttattacc cagttatcaa
    10621 ctaatactct ataaaaactg tctcagagta taattattat cttaaaggag aaaactcatg
    10681 gaagtttttg aaagtctcaa agccaacctt gttggtaaaa atgctcgtat cgttctccct
    10741 gaaggggaag agcctcgtat tcttcaagca acaaaacgct tagtaaaaga aacagaagtg
    10801 attcctgttt tgcttggaaa tcctgaaaaa attaaaattt atcttgaaat tgaaggaatc
    10861 atggatggtt atgaggtcat cgaccctcaa cattatcctc aatttgaaga aatggtttct
    10921 gccttggtgg agcgtcgcaa gggcaaaatg actgaagaag atgtacgcaa ggttttggtt
    10981 gaagatgtca actactttgg tgtgatgttg gtttacttgg gcttggttga tggaatggtg
    11041 tcaggagcga ttcactcaac agcttcaaca gttcgtccag ctctacaaat catcaaaact
    11101 cgtccaaatg taactcgtac ttcaggagcc ttcctcatgg ttcgtggtac ggaacgttac
    11161 ctatttggag actgtgccat taacatcaat ccagatgcag aagccttggc tgaaattgcc
    11221 atcaactcag caatcacagc taagatgttt ggcatcgaac ctaaaattgc catgttgagc
    11281 tattctacta aaggttcagg gtttggtgaa agcgttgata aggtcgttga agcaactaaa
    11341 attgctcacg acttgcgtcc tgaccttgaa atcgatggtg agttgcaatt tgatgcagcc
    11401 tttgttcctg aaactgcagc tctgaaagct cctggaagta cggtagctgg tcaagcaaat
    11461 gtcttcatct tcccaggtat cgaggccgga aatatcggtt acaagatggc tgaacgcctg
    11521 ggtggctttg cggctgtagg acctgttttg caaggtttaa acaagccagt taatgatctt
    11581 tctcgtggat gtaatgcaga tgatgtttac aagttgaccc tcatcacagc agctcaagca
    11641 gttcatcaat agtgaaaact ataaagcgat atactatgct atactgtagt tatgaaacta
    11701 tgtacgaaaa gcactgccat taattcctga gaactaaatt actgattggt gtcaaaaagg
    11761 aaaacttcca agcgatgata tcctgtctat acacgaccta tagaaatctg taatatgtat
    11821 gtccgtaaaa cgataatttc cctttttgat tttaaatgag tatgaaaaga gaattttttc
    11881 atgagaaaac gcataatatt aagtgtcttg acacctgcta ttatgcgttt tctggctctt
    11941 tgtcaactgt agtgggttga agaaaagcta agctcgagaa aggacaaatt ttgtcctttc
    12001 ttttttgata ttcagagcg
//
LOCUS       DAVBCV010000060        12003 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBCV010000060 DAVBCV010000000
VERSION     DAVBCV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.060"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(126..571)
                     /locus_tag="VQ516_001736"
                     /pseudo
     CDS             complement(126..571)
                     /locus_tag="VQ516_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            579..722
                     /locus_tag="VQ516_001737"
     CDS             579..722
                     /locus_tag="VQ516_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512136.1"
                     /translation="MAERFWENLSIILADRNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            815..1402
                     /locus_tag="VQ516_001738"
     CDS             815..1402
                     /locus_tag="VQ516_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2512137.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCSDELTILQILYGASLIS
                     FLWMYGQRKQVIKVNMKTRIQWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            1801..2610
                     /locus_tag="VQ516_001739"
     CDS             1801..2610
                     /locus_tag="VQ516_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2512138.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNNYSCKALC
                     ECLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIREKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKVSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWNSLLLNENCLVIGASVSGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTNHLSYGNRISPQFPILLITDCLFSHYLESPERQYYYNQTIIHKEE"
     gene            2615..3316
                     /locus_tag="VQ516_001740"
     CDS             2615..3316
                     /locus_tag="VQ516_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2512139.1"
                     /translation="MSQIWTKEKFISQVHGGVIVSCQALPGEPLYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVINEFIKKIKEKYPNQLLMADVSNFDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            3341..4258
                     /locus_tag="VQ516_001741"
     CDS             3341..4258
                     /locus_tag="VQ516_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2512140.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            4286..5827
                     /locus_tag="VQ516_001742"
     CDS             4286..5827
                     /locus_tag="VQ516_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET2512141.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDDRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDIPEIKADSS
                     WEVRH"
     gene            5846..6304
                     /locus_tag="VQ516_001743"
     CDS             5846..6304
                     /locus_tag="VQ516_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2512142.1"
                     /translation="MIISEQSDFRRYASINKHFSKVCDFLENTNLTDLVDGKVDIDGE
                     NVFANCMIYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDEPVKKLVIKVLNEEK"
     gene            6306..8528
                     /locus_tag="VQ516_001744"
     CDS             6306..8528
                     /locus_tag="VQ516_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET2512143.1"
                     /translation="MKKNIKQYVTLGTAVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTPISEVQPKSGNSSEVTVQPRTVETTVKDPSSATEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFKNNKKTEYRNGTHVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSTDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            8568..9677
                     /locus_tag="VQ516_001745"
     CDS             8568..9677
                     /locus_tag="VQ516_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2512144.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            9834..10724
                     /locus_tag="VQ516_001746"
     CDS             9834..10724
                     /locus_tag="VQ516_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2512145.1"
                     /translation="MKIIGIDIGGTTIKADLYNEFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYILNHSIDGVGISTAGVVNANTGEIIYTGYTIPGYIGVNFTFEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRAGDKVAKETFEIFVE
                     NLTKGLLTITYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            10749..11072
                     /locus_tag="VQ516_001747"
     CDS             10749..11072
                     /locus_tag="VQ516_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512146.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENYEKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            11062..>12003
                     /locus_tag="VQ516_001748"
     CDS             11062..>12003
                     /locus_tag="VQ516_001748"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2512147.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQISHTSNSNHGVIEGNDALIYLMNQQQYLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNRQINRVHELERHIQSNNEEIERLIKW
                     EKLEIIPAHLEQFFFCKGKVGTIPRTVDNRLYNSFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKNNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETR"
BASE COUNT         4078 a         1756 c         2347 g         3822 t
ORIGIN      
        1 gttgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaagag
       61 cctgggacaa aatagttctc gaccacaaaa aagctagaga tttccaattg tggaacttta
      121 gctttttaat tttgagtcgt tctcttattg tattttagga ggttattggc cataagtacc
      181 aatcccatgt caattctcac ttgacgcttg cccctcagat tacatctctt gtaacccaaa
      241 caagccttta tctgcccaaa gacaggtgac attctttagc tttcaagttt tgatagcctt
      301 cgttcatata cagtcccttt tgaggggctg attcaggttc gtcggcgtag taaaccttga
      361 tttcttgttt aaagtctgtc tgtgttttct gatgtttggt atggtgaaaa cgataatacc
      421 agccatcagg atgtgtatag ctatcctcct tgtcattata gtaccaattc gctaagtttc
      481 tagctgactg tttatagcct ctcttctgtt ccttatcaaa catggcatat ttaatcagat
      541 ggtttacctc cttttcatct aactaaagga gaagcaatat ggctgaacga ttttgggaga
      601 acttgtccat tattttggct gacagaaata tcagctggat tgagttaacc agaaaaatgt
      661 ttgcgggaga gtttcactat ccaagtgaat tgaatcgttt gtatcaaaag attcgccact
      721 agaagatgga acaacgaatg cctcaaagtc catgggtaga aaggattgtg caggtattag
      781 atttagatta tgaagattta tttcggaggt aattatgaaa agaataattc cagtttatat
      841 attccaacaa gtaaatgtcc tattggtatc tctatactta ttgaaatttc tttgtagcga
      901 tgagttaact atactacaga ttctctatgg agcgtcactc atttcttttt tatggatgta
      961 tggtcaacga aaacaagtga tcaaggttaa tatgaaaact aggatacaat ggcttggtat
     1021 tggattcgtt agcctactga ttataagtct atgttttagt ctaatccatg cgcaagaaag
     1081 cacgaatcaa gcaaacttaa ttggacttca acatcaaatt ccttggtttt catttttatt
     1141 gtttttaatc aatgcgagta tggttgaaga atttctgtat agagaaattt tatggaactt
     1201 ggttagaaaa ttagatattc gagttgcttt gacaagtgtt ttatttgcct tagcacatca
     1261 tccaggaacc attatagctt ggtgtttgta tgtatcgctg gggatgtttt tagggcttgt
     1321 acgctacaaa ttggacttat ggggcagtat ggggctacat ttggtgtgga acctactagt
     1381 ttatagtttg ctgctttttt aaaataagtg atctgattta tctcataaat cagtagtttt
     1441 tataggatta tttttaattc ggaaacaaaa atgtattaac agcataaaat aaagaaacta
     1501 ggaacatttt ttttattttt tagaaataaa atgttgtata gaaattccga aagaaataat
     1561 aaaatcaaaa taaaaattaa taaaataggt atatttgaac atattgacgt cgacaaatgt
     1621 tcttgcaaga cataaaatac aaataaaaag aataaaaaat ttttatattt ttatatttgt
     1681 tgttctgtta taaaatgaaa gaaattagaa actagtttct aaatcatgaa aaatattgaa
     1741 aaaaaaaaaa taagtgttaa aataaagaat gtaaacgttt acttcaataa aggagctcat
     1801 atgttactgc aaaaagaact aattccaatg atagaagcta acttaccaaa tatggcatat
     1861 gctgaaaaag acattgctaa attcttctta aaacagcaac ctctgaataa ttattcatgt
     1921 aaggcattgt gcgaatgcct taatgtatcc aaagcaacat tgactcgatt tgcgaaaaaa
     1981 tgtggtttta aaggttttag acaattcatt ttcaaatacc aagagatgat tcgtgagaaa
     2041 gaaaagttag cattgtatac agaggctaca gaaaaagttt tatccgacta tgaagaaatg
     2101 ttgagaaaaa cttacacggt tcttgatgaa gttcaacttg agcgtattgc tgagatgata
     2161 gaaactgctg agcgtgtata tctttatggt aaagtaagtt ctgttcttgc tttacaagaa
     2221 atgaagatga gatttatgcg tctcggagtc attggtgaag tattatcaga cgaggatatg
     2281 attttgtgga atagcttatt acttaatgaa aattgccttg tcattggagc atccgtttca
     2341 ggtcaaactg atattgtact agaaggtctt caaaaagctg cggataaagg cgctaaaaca
     2401 gttttaatga ctacaagaaa atttgacgaa gaagattgtt tctttgatga actattgtta
     2461 ttagcttcga ccaatcatct ctcgtatggc aatcgcatat cacctcagtt tccaatactt
     2521 ttaattacag actgcttatt ctctcattat ctggaaagtc cagagagaca atattattac
     2581 aatcaaacta ttatccataa ggaggaataa taaaatgagt caaatttgga ctaaagaaaa
     2641 atttataagt caagtccatg gtggagtcat tgtttcttgt caagctttac ccggtgaacc
     2701 cctttataat gaagaattta gcttgatgcc ttttatggct aaagcagctt tagaggcagg
     2761 agcagtgggc attcgcgcaa attctgtgcg tgatattaaa gcaattcaga aagtagtaga
     2821 tttaccaata attggaatta tcaaaagaga ttatccacct caagaaccat atattactgc
     2881 tacgatgaaa gaagtagatg aacttgtaga atgcggaaca acagtcattg catttgatgc
     2941 aactttaaga ccaagatatg atggcttagt tatcaatgaa tttatcaaaa aaataaaaga
     3001 aaaatatcct aatcaattgt tgatggcgga tgtaagtaat tttgatgaag gtctctatgc
     3061 atttaaatca ggcgttgatt ttgttggtac aacattatca gggtacacaa gtacaagtgt
     3121 acaatcagat gagcctgatt ttgaactaat gaaaaaattg gctgatttta atattccggt
     3181 aattgccgaa ggaaaaattc attatccaga acaattaaaa aaagcttata gtttaggtgt
     3241 taccagtgtt gtcattggtg gagcgattac acgtccaaaa gaaattgctc agcgatttat
     3301 taatgtcatc aaataaaaaa caatataaag gagaaatctg atgaaagatt taactaaata
     3361 caaaggcgtt attcctgcat tttatgcttg ctatgatgaa aatggtgaaa ttagccaaga
     3421 acgtgtaaaa tctctggtgc aatatttcat tgacaaaggt gtaaaaggta tctatgtaaa
     3481 tggttcttca ggtgaatgta tttaccaaag tgtagaagat cgtaaacaaa ttattgaagc
     3541 tgttatggaa gttgctaaag gtaaattaac agttatcaac catgttgcat gtaataacac
     3601 gaaagatagt attgaattgg caaaacattc agaaagtgtt ggagtcgatg ctattgctgc
     3661 tatcccacct atttacttca aattgccaga atattcaatt gcagcatatt ggaatgcaat
     3721 gagtgaagct gcgccaaata cagattttat catttataat attccacaat tggcaggggt
     3781 tgcgttgact ggtagtttgt atgcaacaat gcgtcaaaat cctcgtgtga ttggagttaa
     3841 aaattcttct atgcctgtac aagatattca aatgtttgta gctgcaggtg gagaagatta
     3901 cattgtattc aatggtccag atgaacaata tcttggtggt cgcttgatgg gagcagaagc
     3961 tggtattggt ggtacttatg gcgttatgcc agatttgttc ttgaaattgg aaagtttgat
     4021 tcaagaacga gatttagata ctgctaaaaa acttcaatat gctatcaatg aagttatcta
     4081 taagatgata tcaggtaagg caaatatgta tgctgtagca aaagaggttt tgcgtctaaa
     4141 tgaaaaactt gatttaggtt ctgttcgtca acccttagaa gcattggcag aaggtgactt
     4201 ggaagttgca aaacaagcag cagaacttat tcaacaagca cgaaaagaat ttttataata
     4261 aataaaatca atatggagat atgttatggg cacaacagga tttacaataa ttgacttaat
     4321 tatcttgatt gtttatttac ttgcggtgtt ggttgcaggt atctatttct ctaaaaaaga
     4381 gatgaaagga aaagagttct tcaaaggaga tggttcggtt ccttggtatg ttacttcggt
     4441 atccattttt gccacaatgc tcagtccgat ttccttcttg ggactcgctg gtaactctta
     4501 tgcaggtagc tggattttat ggtttgctca attagggatg gtagtagcta ttccactgac
     4561 aattcgtttt atcttaccta tctttgcacg gatagacatc gatacggcat atgattactt
     4621 ggataaacgt tttaattcta aagcacttcg tattatttca gcactcttgt ttattattta
     4681 tcaattggga cgtatgtcta tcattatgta cctcccatca gctggtttat cagtattgac
     4741 aggaattgac atcaatattt tgattatttt gatgggtgca gttgcaattg tttattctta
     4801 cactggtggt ctaaaatccg tattatggac agactttatt caaggtgtga ttctgattag
     4861 tggtgtcgtt ttagctttat ttgtactgat ggctaatatt aaaggtggct ttggtgcagt
     4921 agcagaaaca ttagcaaacg ggaaattcct tgctgcaaat gaaaaacttt tcgatcctaa
     4981 cttgctttca aactccatct ttttaattgt gatgggttca ggctttacaa tcttgtcttc
     5041 ctatgcttca tctcaagatt tggttcaacg ttttactaca acacaaaata ttaagaaact
     5101 taataagatg ttgttcacaa acggtgtctt gtcacttgca actgcaacag tcttttactt
     5161 gattggtaca ggcttgtacg tattctatca agtacaaaat gcaggtagtg cagctagcaa
     5221 tatccctcaa gaccaaatct ttatgtactt tattgcatac cagttaccag taggtatcac
     5281 aggtttgatt ttggcagcga tttatgcagc atctcaatca actatttcaa caggtttgaa
     5341 ctctgttgca acttcatgga cattggatat tcaagatgtc atttctaaaa atatgtcaga
     5401 cgatcgtcgt acgaaaattg cacaattcgt atctctagca gtaggtttat tctcaattgg
     5461 tgtttccatt gtcatggctc actcagatat taaatctgca tacgaatggt tcaatagctt
     5521 catgggactt gtacttggtc tacttggtgg tgtatttatt cttggatttg tttctaaaaa
     5581 agcaaataaa caaggtgctt atgcagcgct gattgtatca actatcgtca tggtatttat
     5641 taaatacttc cttcctccaa cagctgtgag ctactgggca tattcattga tttcaatctc
     5701 tgtatcagta gtttcaggtt atattgtatc tgttcttact ggaaataaag tatctgcacc
     5761 taaatataca acgattcatg atattccaga aattaaagcg gattcaagtt gggaagttcg
     5821 tcactaatag aaaagagaaa ataatatgat tatttctgaa caatctgatt ttagacgata
     5881 tgcttctatc aataaacatt tttcaaaagt ctgtgatttt ttagaaaata caaatttgac
     5941 agatttagtt gatggaaaag ttgatattga cggggaaaat gtttttgcaa attgtatgat
     6001 ctatctagct gatggagtcc caggagatat ttttgaaact cataagaaat atttagatat
     6061 tcatattgtt gttgaaaata ctgaaaaaat ggcagtaact tccccagttc gtgcccagtc
     6121 acgtgtacct tttagcgaag aaaaggatat tgctttttac gatagcaaag actatcaaat
     6181 tgttgaattg cttccaggga atatgttggt gacttttgaa gaagatcttc accaaccaaa
     6241 gattcattgc aatgatgagc ctgttaaaaa acttgttatt aaagttttaa acgaggaaaa
     6301 ataaaatgaa aaaaaatatt aaacaatacg taaccttagg tactgcagtg gtattatcag
     6361 catttgttgc taactcagtt gcagctcagg agactgaaac ttctgaagta tcaacaccag
     6421 agttggtgca acctgttgca ccaacgactc cgatttcgga agtacaacct aaatcgggta
     6481 actcttcgga agttactgta caacctagaa cagtcgaaac tactgttaag gatccatctt
     6541 ctgcaacgga agaaactcct gtcttagaaa aaaataatgt tactttaaca gggggcggag
     6601 aaaatgttac taatgagtta aaggataaat ttactagcgg tgactttact gtagtgatta
     6661 agtacaatca gtctagtgag aaaggcttac aagctctgtt tggaatatct aattctaaac
     6721 ccggtcaaca aaatagttat gtagatgtgt tccttagaga caatggtgaa ttgggaatgg
     6781 aagcgcgtga tacttcttcc aataaaaatc acctagtatc cagacctgct tcagtttggg
     6841 gtaagtacaa acaagaagct gtgactaaca ctgttgcagt agtagcagat tcagtcaaaa
     6901 aaacatattc tttatatgca aatggtacaa aagtagtaga aaagaaagtg gataacttct
     6961 taaacatcaa ggatattaaa ggtattgatt actatatgct tgggggagtg aaacgtgcag
     7021 gaaaaacggc gtttggtttt aacggaacac tagaaaatat caaattcttt aatagtgcat
     7081 tggatgaaga aactgttaaa aagatgacaa caaacgctgt tactggacat ttaatttata
     7141 cggctaatga tacaacaggt tctaactatt tccgtattcc agttctgtat acttttagca
     7201 atggtcgggt attttcaagc attgacgctc gttacggcgg aactcatgat tttttgaata
     7261 aaattaatat tgctacaagt tatagtgatg ataatggtaa gacatggact aaaccaaaat
     7321 taacattggc attcgatgat tttgcgccag taccattaga atggcctcgt gatgttggtg
     7381 gacgtgactt acaaatcagc ggtggtgcaa cctatattga ctctgttatt gttgaaaaaa
     7441 ataacaaaca agtactcatg tttgcggatg tgatgcctgc tggagtaagt tttagagaag
     7501 ctactagaaa agattcaggt tataaacaaa ttgatggtaa ttattacctt aaattaaaga
     7561 aacaaggtga tactgattac aattatacta ttcgtgagaa tggtactgta tacgacgatc
     7621 gtaccaacag accaactgaa ttttcagtag ataaaaattt cggtattaaa caaaatggta
     7681 attatttgac agtagagcaa tattcggttt catttaaaaa taataaaaag actgaatatc
     7741 gtaatgggac tcacgtccat atgaatatct tctacaaaga tgcattgttc aaagtagtgc
     7801 caacgaacta tattgcatat atttctagca atgatcatgg cgaatcttgg tctgcaccaa
     7861 ctttattacc tcctataatg ggacttaatc ggaatgcgcc ttatttgggt cctggacgtg
     7921 gaatcattga aagctcaact ggacgtattc ttattccgtc ttacactggt aaagagtctg
     7981 cattcattta tagtgacgat aatggagcat cttggaaagt taaagttgtg ccacttcctt
     8041 ctagttggtc agcagaagca caatttgtag aattgagtcc aggagtaatt caagcataca
     8101 tgcgtacaaa taatggtaaa attgcatatt taacaagtac agacgcaggt actacttgga
     8161 gtgcaccgga atatttgaaa tttgtttcaa atccaagtta tggaacacaa ttatcaatca
     8221 tcaattatag tcaactgatt gatggtaaaa aggctgtcat tttaagtact ccaaactcca
     8281 caaatggtcg taaacacgga caaatttgga ttggtctaat taatgatgat aatacaattg
     8341 attggcgtta tcatcacgac gttgattata gtaactatgg atactcatat tcaacattga
     8401 cagagttacc aaatcatgaa attggattga tgtttgaaaa atttgattca tggtctcgta
     8461 atgaacttca tatgaaaaat gttgtaccat atataacatt taagattgaa gatctgaaaa
     8521 agaattaaag ctgaaatttg aaaatatata aaaagaggat aaaaattatg gtaaattacg
     8581 gtattgttgg agctggatat tttggagctg atttagctcg ctcaatgaac aaaattgaag
     8641 atgcaaaagt ggttgcggta tttgacccaa atcatggaga agaagttgct caagagttgg
     8701 gatcagatgt ttgtgcaagt ttagatgaac ttgtagcacg tgaagatatt gattgtgtga
     8761 tcgtagcttc acctagctac cttcaccgtg aaccagttgt gaaagctgct caacatggca
     8821 aacacgtatt ttgtgaaaag ccaattgcat tgtcttatga agattgtaaa gccatggttg
     8881 acgcatgtaa agaaaataat gttatcttta tggctggtca catcatgaac ttctttaacg
     8941 gtgtacacca tgctaaagaa ttgattactc aaggtaaaat cggtaaagtt ctttattgcc
     9001 atgccgctcg tacaggttgg gaagaacaac aaccaactgt atcatggaag aaacttcgtt
     9061 ctcaatctgg aggacatttg taccatcata ttcatgaatt agattgcatt cagtttatca
     9121 tgggaggact tcctgaaaaa gcgacaatgg taggaggcaa tgtatatcat aaaggtgaaa
     9181 actttggtga tgaagatgat atgctcattg taaacttaga atactctgat gatcgttatg
     9241 ctgttttgga atatggtaat gctttccgtt ggggtgaaca ctacgtcttg attcaaggaa
     9301 ctgaaggagc tatcaaactt gatttgttca atactggagg tactcttcgt gttaaaggtg
     9361 aaggagaatc acacttctta gttcatgaaa ctcaagagga agatgatgac cgtacagcta
     9421 tctataccgg tcgtggtatg gatggagcaa ttgcgtacgg taaaccagga gtacgttgcc
     9481 cattatggtt gcaaacatgt attgataaag aaatggaata tctacatgac atcattaaag
     9541 gtggagaaat tacagaagaa tatgaaaaac ttctcaatgg tgtagctgct ttagaatcaa
     9601 tcgctaccgc tgatgcatgt actttatcag ttaaagaaga tcgaaaagta agtctttcag
     9661 aaatcacaaa tgcttaactt ttgtaaaaca gaatagtaaa ttcttatcat tatataattt
     9721 ctaaagttct gtgatacaac tcattgaata aagaaataga gatgggactg ggataatgcc
     9781 cagtcccatt ttttatcaaa aagtaatgag atcaaaaatg tgggagtgat gaaatgaaga
     9841 ttatagggat cgatattggc ggaacaacaa ttaaagcaga tttatacaat gagtttggaa
     9901 cgagtttaaa tcagttcaaa gagatagaaa caattattga ctatgatttg ggaacgaacc
     9961 agatattaaa tcaggtctgt gatttaattg gtgagtatat tttaaatcat tcaattgatg
    10021 gtgttgggat ttccactgct ggagttgtta atgctaatac aggagagatc atctatacag
    10081 gctatacaat accagggtat atcggagtaa actttacttt cgaaatagaa aaacgttttg
    10141 ggttgtctac ttttgttgaa aatgatgtta attgtgctgc attaggtgaa ttgtggaagg
    10201 gacaagccaa agataagaaa aatgtagtaa tggttactat tggaacaggt ataggaggca
    10261 gtattattgt caatggacaa attgttaacg gatttaacta tactgctggt gaagtaggct
    10321 atattcctgt aggtaattcg gattggcaaa gcaaagcctc aacaaccgca ttgattcatt
    10381 tatatcaaaa gaaaagcttg aaaactaatc aaactgggcg tactttcttt actgatttaa
    10441 gagctggaga taaagtcgct aaagaaactt ttgaaatttt tgtagaaaat ctaacaaaag
    10501 gtttattaac gattacttat ctacttaatc cagaaattct catattagga ggtgggatcc
    10561 tggctaaaaa ggatattttg ttacctgaaa ttcaaagttc tttagctaaa aatgcaatgg
    10621 ataataggtt tttgcctaaa aatcttgtgg cagctacatt aggaaatgaa gctggtcgta
    10681 taggagctgt aaaaaatttc ttagatagaa tttctaataa atagtatgaa agataaggag
    10741 gtgtcacaat gactaactct gtattttcga caatgcaaga tattgagaat gttgcaaccg
    10801 atattataaa atcatatgat aatgagattt atacttataa agctgtttcc caagaagaat
    10861 tggaaaaact agaaaaaagt tatgatgaaa aaagtcacga agaattagtt tcaatagaaa
    10921 gcaatttaga aatgaaacaa cagaacctta ttgatgaggt taataaaaca atcaaggaaa
    10981 attatgaaaa gattcagtat atttcatcaa gtaggagagg agaatttgta gaaaaaatta
    11041 ttggtagggt ggtagaaaaa tatggctatt agtcagatga aaagaatttc tctactattt
    11101 tctaaaagta gtcttgatga tgttttaaaa actattcaag aactagagtc agtgcagttc
    11161 cgtgatttaa aggttcagga taactggtca gaagctctag aaaaagatga ggttgtattt
    11221 ccaactattc aaatttctca tacttctaat tccaatcatg gggttattga aggaaatgat
    11281 gccttgattt atttgatgaa tcaacaacaa tatttagaag caactgtaga gaaattacaa
    11341 gaatacctac cgaaagaaaa cacgtttaaa ttattacagc aacctccgat aacgacctct
    11401 tatgaagaat tagaaaaatt tggtaaagct aatgttgctg agggtgttct taaaaaagtg
    11461 aatcgtcaaa taaacagagt tcatgaatta gaaagacaca ttcaaagtaa taatgaggaa
    11521 atagagagat taataaaatg ggaaaaatta gaaattattc ctgcgcattt agaacaattt
    11581 tttttctgta aaggaaaagt cggaacaatt ccaaggactg tagataatcg cttatacaat
    11641 agttttttag aaaacaatat tgaagttcaa gaaatatttt ctaatgatag agagtacggt
    11701 gttgttgttt tctatcagtc cagttgctct attgattttg atgaatactt atttgaacca
    11761 tttgattatt ctagaaagga attaccgaag cagcgagtag tagatttgga tcaagaaaac
    11821 atgcagttaa taactgaaaa agagaatatt atcgcatcgt tgcaagattc aaagaataat
    11881 ttgatagatt tacaatggca aattgactat attttatcta tctatgctcg tcaaatctct
    11941 aagaataacc ttttgagcac tccgcatcta gttgcactag aaggatggat agaagaaact
    12001 cgt
//
LOCUS       DAVBCV010000061        11971 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBCV010000061 DAVBCV010000000
VERSION     DAVBCV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.061"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(118..1749)
                     /locus_tag="VQ516_001749"
     CDS             complement(118..1749)
                     /locus_tag="VQ516_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2512148.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGITITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2042..7021
                     /locus_tag="VQ516_001750"
     CDS             2042..7021
                     /locus_tag="VQ516_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2512149.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAVLASLGLASLALTLRRKREDKD"
     gene            7111..8307
                     /locus_tag="VQ516_001751"
     CDS             7111..8307
                     /locus_tag="VQ516_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2512150.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8443..8970
                     /locus_tag="VQ516_001752"
     CDS             8443..8970
                     /locus_tag="VQ516_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2512151.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9047..9403
                     /gene="glnR"
                     /locus_tag="VQ516_001753"
     CDS             9047..9403
                     /gene="glnR"
                     /locus_tag="VQ516_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2512152.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9440..10786
                     /gene="glnA"
                     /locus_tag="VQ516_001754"
     CDS             9440..10786
                     /gene="glnA"
                     /locus_tag="VQ516_001754"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2512153.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10882..11001)
                     /locus_tag="VQ516_001755"
     CDS             complement(10882..11001)
                     /locus_tag="VQ516_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2512154.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11259..11396
                     /locus_tag="VQ516_001756"
     CDS             11259..11396
                     /locus_tag="VQ516_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512155.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEALNIRLEVK
                     N"
     gene            11489..11971
                     /locus_tag="VQ516_001757"
                     /pseudo
     CDS             11489..11971
                     /locus_tag="VQ516_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3845 a         2183 c         2569 g         3374 t
ORIGIN      
        1 ctattctagt ttcaatatac tatatatctg cagaatactc tttcagtttc attcagccta
       61 gtcttgctta ggaaaaagta aaaagccatc caattatgat ggatagcttc ttggttctta
      121 gatttgttct gcaaaaacct tttcagcaag gttcatagcg tggtctgata cacgagtgta
      181 gtgtgagatg atgtcgataa agttgacccc agcttgtgtt gaacattcgc ctttgttgag
      241 gcgtttgatg tgggtttttc tgagaacacg ttctatctta ttgattgctt catgacgttc
      301 tacaagactg cgtgcttttt caatatcatt gttttccaca ctatccagag catctttgat
      361 aaagtcacta gtttggcggt aaacttcctc taattctttc aaggcggcat cagaaaattc
      421 aacatttttc cgttgaagat agtcagtcag attgagtaga gcctccgtgt ggtctccaat
      481 ccgttccaaa tcacgggagg aatcaaggat attggtaagc acttcacttt ctttttggct
      541 gagagcttcg cttgaaaggg caatgagata acgtgttaat tgctcatcga tggtgttaat
      601 tgcttcttcg gttttatgcc ctttttcagc aactttttca tccaagtcaa tgatgtactt
      661 ataggaaagg tcaaaggctt tagcagcgta gtttcctaag tgcaagagct ctttcttagc
      721 atttcctaga gcgatagatg gggcctgttt gatgaaatgt tcatcaagat ataagggttc
      781 gtatttgaca acctcgtcct ctccaggaat aatcttggtt acaaagtaag ccagagctcc
      841 gataaatgga aattggacaa tggtgttggt aatattaaag gttccgtgag caaaggcgat
      901 ggtcatttcc ggtgctagat ttagcgtagc ttcaaaccaa tgaatcagga cagtaaaagg
      961 aactagaaaa ataacgcaga cgactgttcc gataacgttg aaggcaacat gagctcctgc
     1021 tacccgttta gctgcaatat tagcccctaa agaggcaatg atggctgtaa tggttatccc
     1081 gatattgtca ccaaatagaa ctggcaaagc tccctgtagg tcaattagat tgccggcgta
     1141 gaggttttgt aaaatcccaa tggtagccga agaagcttga attagcaagg tcaagccggt
     1201 accgacaaag acacccaaaa caggattctt acttagctca atcatatagt ccttaaagac
     1261 ctgtaaatcc ttgagtggag ccattgcgcc gctcatgaga ttgagggcaa aaaagatacc
     1321 accgacacca aagaggatgc gtccgatatt attgactgtc cgattttttg taaaaaagag
     1381 acagacggca ccgataaaga gcataggtag ggcatagtta cctaatttaa aaccgatgag
     1441 aaaggatgtg acagttgtcc caatattagc acccatgaca atcccgatag cctgacgtaa
     1501 ggttaagaga ccggcactga ccaggccgac tgtgataact gttacaccag aactagactg
     1561 aattagagca gtcatcccaa taccaaccag aactccaaag aaaggattac tagtatattt
     1621 gtcaatgtaa aaacgaaggc gatctccagc agcttgttgc aaaccgtctc ccatggtctt
     1681 gatgctatat aagaatagcc ccagaccacc taaaaagtga aataaaattt cctgccaatt
     1741 aatggacatt tctttttcct ccgaaaaata atcgcggaat atcttctatt ctattttaaa
     1801 ggataaaagt aaatctaaca agtgttaatg tcaagtttgt aaaagaaaat tataattgat
     1861 aagtgtatta tttttaagag tttttaacct tattttagat ttgtgcaaac agttattgtt
     1921 ttttgtctaa aaatgtttta ggataaaaat gtgattatca tggttctaga gtttgataaa
     1981 aaactacaga atataaagcg ctttcttgaa aacaacaaaa tcaatctttt aggaggagaa
     2041 aatgaagaat ccattttttg aaagacgttg tcgttacagt attcgtaagt tatcagtagg
     2101 agcctgctcg ctgatgattg gtgctgtttt atttgctggt ccagccttgg ctgaagaaac
     2161 tgcagttcct gaaaatagcg gagctaatac agagcttgtt tcaggagaga gtgagcattc
     2221 gaccaatgaa gctgataagc agaatgaagg ggaacatgct agagaaaaca agctagaaaa
     2281 ggcagaagga gtagcgatag catctgaaac tgcttcgcca gcaagcaatg aagctgcaac
     2341 tactgaaact gcagaagcag ctagcgcagc taaaccagag gaaaaagcaa gtgaggtggt
     2401 tgcagaaaca ccatctgcag aagcaaaacc taagtctgac aaggaaacag aagcaaagcc
     2461 cgaagcaact aaccaagggg atgagtctaa accagcagca gaagctaata agactgaaaa
     2521 agaagtccag ccagatgtcc ctaaaaatac agaaaaaaca ttaaaaccaa aggaaatcaa
     2581 atttaattca tgggaagaat tgttaaaatg ggaaccaggt gctcgtgaag atgatgctat
     2641 taaccgcgga tctgttgtcc tcgcttcacg tcggacaggt catttagtca atgaaaaagc
     2701 tagcaaggaa gcaaaagttc aagccttatc aaacaccaat tctaaagcaa aagaccatgc
     2761 ttctgttggt ggagaagagt tcaaggccta tgcttttgac tattggcaat atctagattc
     2821 aatggtcttc tgggaaggtc tcgtaccaac tcctgacgtt attgatgcag gtcaccgtaa
     2881 cggggttcct gtatacggta cactcttctt caactggtct aatagtattg cagatcaaga
     2941 aagatttgct gaagctttga agcaagacgc agatggtagc ttcccaattg cccgtaaatt
     3001 ggtagacatg gccaagtatt atggctatga tggctatttc atcaaccaag aaacaactgg
     3061 agatttggtt aaacctcttg gagaaaagat gcgccagttt atgctctata gcaaggaata
     3121 tgctgctaag gtaaaccatc caatcaagta ttcttggtac gatgccatga cctataacta
     3181 tggacgttac catcaagatg gtttgggaga atacaactac caattcatgc aaccagaagg
     3241 agataaggtt ccggcagata acttctttgc taactttaac tgggataagg ctaaaaatga
     3301 ttacactatt gcaactgcca actggattgg tcgtaatcct tatgatgtat ttgcaggttt
     3361 ggaattgcaa cagggtggtt cctacaagac aaaggttaag tggaatgaca ttttagacga
     3421 aaatgggaaa ttgcgccttt ctcttggttt atttgcccca gataccatta caagtttagg
     3481 aaaaactggt gaagattatc ataaaaatga agatatcttc tttacaggtt atcaaggaga
     3541 ccctactggc caaaaaccag gtgacaaaga ttggtatggt attgctaacc tagttgcgga
     3601 ccgtacgcca gcggtaggta atacttttac tacttctttt aatacaggtc atggtaaaaa
     3661 atggttcgta gatggtaagg tttctaagga ttctgagtgg aattatcgtt cagtatcagg
     3721 tgttcttcca acatggcgct ggtggcagac ttcaacaggg gaaaaacttc gtgcagaata
     3781 tgattttaca gatgcctata atggcggaaa ttcccttaaa ttctctggtg atgtagccgg
     3841 taagacagat caggatgtga gactttattc tactaagtta gaagtaactg agaagaccaa
     3901 acttcgtgtt gcccacaagg gaggaaaagg ttctaaagtt tatatggcat tctctacaac
     3961 tccagactac aaattcgatg atgcagatgc atggaaagag ctaacccttt ctgacaactg
     4021 gacaaatgaa gaatttgatc tcagctcact agcaggtaaa accatctatg cagtcaaact
     4081 atttttcgag catgaaggtg ctgtaaaaga ttatcagttc aacctcggac aattaactat
     4141 ctcggacaat caccaagagc cacaatcgcc gacaagcttt tctgtagtga aacaatctct
     4201 taaaaatgcc caagaagcgg aagcagttgt gcaatttaaa ggcaacaagg atgcagattt
     4261 ctatgaagtt tatgaaaaag atggagacag ctggaaatta ctaactggct catcttctac
     4321 aactatttat ctaccaaaag ttagccgctc agcaagtgct cagggtacaa ctcaagaact
     4381 gaaggttgta gcagtcggta aaaatggagt tcgttcagaa gctgcaacca caacctttga
     4441 ttggggtatg actgtaaaag ataccagcct accaaaacca ctagctgaaa atatcgttcc
     4501 aggtgcaaca gttattgata gtactttccc taagactgaa ggtggagaag gtattgaagg
     4561 tatgttgaac ggtaccatta ctagcttgtc agataaatgg tcttcagctc agttgagtgg
     4621 tagtgtggat attcgtttga ccaagccacg taccgttgtt agatgggtca tggatcatgc
     4681 aggagctggt ggtgagtctg ttaacgatgg cttgatgaac actaaagact ttgaccttta
     4741 ttataaagat gcagatggtg agtggaagct agctaaggaa gtccgtggta acaaagcaca
     4801 cgtgacagat atcactcttg ataaaccaat cactgctcaa gactggcgct tgaatgttgt
     4861 cacttctgac aatggaactc catggaaggc tattcgtatc tataactgga aaatgtatga
     4921 aaagcttgat actgagagtg tcaatattcc gatggccaag gctgcagccc gttctctagg
     4981 caataacaag gtacaagttg gctttgcaga tgtaccggct ggagcaacta ttaccgttta
     5041 tgataatcca aattctcaaa ctccgctcgc aaccttgaag agcgaagttg gaggagacct
     5101 agcaagtgca ccattggatt tgacaaatca atctggtctt ctttattatc gtacccagtt
     5161 gccaggcaag gaaattagta atgtcctagc agtttccgtt ccaaaagatg acagaagaat
     5221 caagtcagtc agcctagaaa caggacctaa gaaaacaagc tacgccgaag gggaggattt
     5281 ggaccttaga ggtggtgttc ttcgagttca gtatgaagga ggaactgagg acgaactcat
     5341 tcgcctaact cacgcaggtg tatcagtatc aggttttgat acgcatcata agggagaaca
     5401 gaatcttact ctccaatatt tgggacaacc agtaaatgct aatttgtcag tgactgtcac
     5461 tggccaagat gaagcaagtc cgaaaactat tttgggaatt gaagtaagtc aggaaccgaa
     5521 aaaagattac ctagttggtg atagcttaga cttgtctgaa ggacgctttg cagtggctta
     5581 tagcaatgac accatggaag aacattcctt tactgatgag ggagttgaaa tttctggtta
     5641 cgatgctcaa aagactggtc gtcaaacctt gacgcttcgt taccaaggtc atgaagtcaa
     5701 ctttgatgtt ttggtatctc caaaagcagc attgaacgat gagtacctca aacaaaaatt
     5761 agcagaggtt gaagctgcta agaacaaggt ggtctataac tttgcttcac cagaagtaaa
     5821 agaagctttc ttgaaagcaa ttgaagcggc cgaacaagtg ttgaaagacc atgaaactag
     5881 cacccaagat caagtcaatg accgacttaa taaattgaca gaagctcata aagctctgaa
     5941 tggtcaagag aaatttaagg aagaaaagac agagcttgat cgtttaacag gtgaggttca
     6001 agaactcttg gctgccaaac caaaccatcc ttcaggttct gccctagctc cgcttcttga
     6061 gaaaaacaag gccttggttg aaaaagtaga tttgagtcca gaagagcttg caacagcgaa
     6121 acagagtcta aaagatctgg ttgctttatt gaaagaagac aagccagcag tcttttctga
     6181 tagtaaaaca ggtgttgaag tacacttctc aaataaagag aagactgtca tcaagggttt
     6241 gaaagtagag cgtgttcaag caagtgctga agagaagaaa tactttgctg gagaagatgc
     6301 tcatgtcttt gaaatagaag gtttggatga aaaaggtcaa gatgttgatc tctcttacgc
     6361 ttctattgtg aaaatcccaa ttgaaaaaga taagaaagtt aagaaagtat ttttcttacc
     6421 tgaaggcaaa gaggcagtag aattggcttt tgaacaaacg gatagtcatg ttatctttac
     6481 agcaccacac tttactcatt atgcctttgt ttatgaatct gctgaaaaac cacaacctgc
     6541 taaaccagca ccacaaaaca aagtccttcc aaaacctact tatcaaccgg cttctgatca
     6601 acaaaaggct cctaaattgg aagttcaaga ggaaaaggtt gcctttcatc gtcaagagca
     6661 tgaaaatgct gagatgctag ttggggaaca acgagtcatc atacagggac gagatggact
     6721 gttaagacat gtctttgaag ttgatgaaaa cggtcagcgt cgtcttcgtt caacagaagt
     6781 catccaagaa gcgattccag aaattgttga aattggaaca aaagtaaaaa cagtaccagc
     6841 agtagtagct acacaggaaa aaccagctca aaatacagca gttaaatcag aagaagcaag
     6901 caaacaattg ccaaatacag gaacagctga tgctaatgaa gccctaatag cagtcttagc
     6961 cagccttggt cttgctagtt tagccttgac cttgagacgg aaaagagaag ataaagatta
     7021 aatatcgaaa aatcttgtga aatctttcct tatatttcca aagtgtgata taatagtttc
     7081 gaataaaata aataaaggag tttttgtaac atggcaaaac ttactgttaa agacgttgac
     7141 ttgaaaggta aaaaagtcct cgttcgtgtt gacttcaacg taccattgaa agatggcgta
     7201 atcactaacg ataaccgtat cacagcagct cttccaacta ttaagtacat catcgaacaa
     7261 ggtggacgtg caattctttt ctctcacctt ggacgtgtga aagaagaagc tgataaagct
     7321 ggcaaatcac ttgctcctgt agcagctgac ttggcagcaa aacttggtca agatgttgtt
     7381 ttcccaggtg tcactcgtgg tgctgaattg gaagcggcaa tcaacgctct tgaagatgga
     7441 caagttctct tggttgaaaa cactcgttac gaagatgttg acggcaagaa agaatctaaa
     7501 aacgatcctg aacttggtaa atactgggca tcacttggag atggtatctt cgtaaacgat
     7561 gcattcggta cagctcaccg tgcacacgca tctaacgttg gtatctcagc aaacgttgaa
     7621 aaagcagttg ctggtttcct tcttgaaaac gaaattgcct acatccaaga agcagttgaa
     7681 actccagaac gtccattcgt ggctatcctt ggtggttcaa aagtttcaga caagatcggt
     7741 gttatcgaaa acttgcttga aaaagctgat aaagtcctta tcggtggtgg gatgacttac
     7801 acattctaca aagcacaagg tatcgaaatc ggtaactcac ttgtagaaga agacaaattg
     7861 gatgttgcga aagctcttct tgaaaaagca aatggtaaat tgatcttgcc agttgactca
     7921 aaagaagcta acgcatttgc tggttacact gaagtgcgtg acactgaagg tgaagcagtt
     7981 tctgaaggct tccttggtct tgacatcggt ccaaaatcta tcgccaaatt tgacgaagct
     8041 ttgactggtg ccaaaacagt tgtatggaac ggacctatgg gtgtatttga aaacccagat
     8101 ttccaagctg gtacaatcgg tgtgatggac gctatcgtga aacaaccagg agttaaatca
     8161 atcatcggtg gtggtgactc agctgccgca gcgattaacc ttggccgtgc agacaagttc
     8221 tcatggatta gtacgggtgg tggagcatca atggaacttc ttgaaggtaa ggttcttcca
     8281 ggacttgcag ccttgacaga aaaataagat tttataaaca aatcaaagaa gagagggatg
     8341 aaagttcctc ttttcttttg cttaaaataa aaacgcttcc tctcaactat tactcataaa
     8401 aatcaccgat ttatgataaa atggaaatag aaagttgaga ttatgagtta ttttaaaaaa
     8461 tataaattcg ataaatccca gttcaaactt ggtatgcgaa cctttaaaac aggtattgct
     8521 gtttttctag ttctcttgat ttttggcttt tttggctgga aaggtcttca aattggtgct
     8581 ttgacagccg tttttagcct gagggagagt tttgatgaga gtgttcattt tgggacttcg
     8641 cgtattctag gaaatagtat cggtggactc tatgccttgg tcttcttctt attaaatact
     8701 tttttccacg aagccttttg ggtgaccttg gtagttgttc caatctgcac catgttaacc
     8761 attatgacaa atgtagccat gaataacaaa gcaggggtta ttggtggtgt agcagctatg
     8821 ttaatcatta ccctatcaat tccaagtggt gagacaattt tgtacgtgtt tgtgcgtgta
     8881 ttagaaacgt ttatgggagt ttttgtcgca attatcgtaa attacgatat tgatcgtatt
     8941 cgtctctttt tagagaaaaa agaaaaataa tgttacattt tataacatta tcaattgacg
     9001 tttgtctttt tttagactat aatagacaga aagaaggaaa ttgtaaatga aggaaaaaga
     9061 atttcgccga aatatggctg tttttcctat cggcagtgtt atgaagttga ccgatttatc
     9121 ggcgcgtcag attcgttatt atgaagatca agagttgatc aagcccgatc gaaacgaagg
     9181 aaatcgtcgc atgtattcct tgaatgacat ggatcgtctg cttgaaatca aagattatat
     9241 ctctgaaggt tataatatcg ctgccattaa gaaaaaatat gctgaacgtg aagcgaaatc
     9301 caagaaagcg gttagtcaga ctgaagtacg tcgtgcactt cacaatgaac tcctccaaca
     9361 ggggcgtttt gcttcagtac agtcaccttt tggtcgcggt taggcaaccg caagtagtca
     9421 tagattaagg agaaaactta tgccaatcac agctgcagat attcgtcgtg aagtcaagga
     9481 aaaaaatgtt acctttattc gtcttatgtt ctcagatatt ttgggaacca tgaaaaacgt
     9541 cgaaattcct gctacagatg aacagttaga taaggtcttg tcgaacaagg ttatgtttga
     9601 tggatcttct attgaaggtt ttgtacgtat caatgagtcg gatatgtact tgtacccgga
     9661 cttggataca tggacagtct tcccttgggg agatgaaaat ggaagtgttg caggtctgat
     9721 ctgtgatgtc tatacaacag aaggtgaacc atttgcgggt gaccctcgtg gtaatttgaa
     9781 acgagctctt cgtcacatgg aagaagttgg attcaaatcc ttcaaccttg gtccagagcc
     9841 agaattcttc ctatttaagt tggatgaaaa tggggaccca acacttgaag tgaatgacaa
     9901 gggtggctac tttgatttgg cacctactga ccttgcggac aacacacgtc gtgagattgt
     9961 gaatgtcttg accaaaatgg gatttgaagt agaagcgagt caccacgagg ttgcggttgg
    10021 acagcatgag attgacttta agtacgatga agttctccgt gcttgtgata agattcaaat
    10081 ctttaagctt gttgttaaaa ccattgctcg caaacacgga ctttacgcaa catttatggc
    10141 gaagccaaaa tttggtattg ctggatcagg tatgcactgt aatatgtcct tgtttgatgc
    10201 agaaggaaat aacgccttct ttgatccaaa tgatccaaaa ggaatgcagt tgtcagaaac
    10261 agcttaccat ttcctaggcg gtttgatcaa gcatgcttac aactatactg ccatcatgaa
    10321 cccaacagtt aactcataca aacgtttggt tccaggttat gaagcgcctg tttacattgc
    10381 ttgggctggt cgtaaccgtt cgccacttgt gcgcgtacct gcttcacgtg gtatgggaac
    10441 tcgtcttgag ttgcgttcag tggatccaat ggcgaaccct tacattgcta tggctgttct
    10501 tttggaagtt ggtttgtatg gtattgaaaa taaaatcgaa gcaccagctc ctatcgaaga
    10561 aaatatctac atcatgacag cagaagagcg caaggaagct ggtattacag accttccatc
    10621 aactcttcac aacgctttga aagctttgac agaagatgaa gtggttaaag ctgctctcgg
    10681 agatcacatc tacactagct tccttgaagc caaacgaatc gaatgggcaa gttatgcaac
    10741 cttcgtttca caatgggaaa ttgataatta tttagacctt tactaatact aatatagaag
    10801 aaagattgcc tgtgggtaaa cagtcacaca gcagtctaag ccatcaaata cctataaccc
    10861 ttaaaatagc gatttcggtc gctatttatt tttcgaaaaa tcctcttgac tcttttgcga
    10921 attagattta tcatcagacg tgtagaagaa aatcaaggag tcactatgga aaaattcaat
    10981 ttcaaaaaca atatcgggca agaaaacaag ttacttcaaa tcgaaatcta caagtttact
    11041 aacttttgca agcttcaaaa ttacacatct gtaaatattt tttcgaaaga tatatttgaa
    11101 gccatagtaa attgagacta gaatagtaca ctacatctgc taaaaatttc tagaaattaa
    11161 tttaactttc ctaatcgatt tgtttacatc ttatttcaat ctactatatt atctcaataa
    11221 atgatgatgg cgactgtttt gtttgtgctt gtcgattttt gaatcctgat cgagcggaag
    11281 aatatttctg ccgaggttgt caaggggaaa atcctgaaga tattgaattt tatgatgaac
    11341 aattacaggc agaaaaggtg gaggctttga atattcgatt ggaagtaaaa aattgaatta
    11401 tctacaatct catcaacaat aaacctattt caaccccatt atctataggc gtatttttac
    11461 gcctttttat atacttgaag cacataagaa acattatggg aagtttgctg atggaagtac
    11521 tcaagaaatt gatgttcctt atgatattcc tgatacttgg gagtgggtga ggataaaatc
    11581 aatttattgg aattttgggc aaaataagcc agagaaatcc tttaggtata tagatacgtc
    11641 tagtattgat agaaaaaaga acataatcaa ctacaaaaat ctacaatatc tttcacctga
    11701 acaagcgcct tcccgtgcca gaaaattagt ttcgcagaat agtgtcttat tttcaacagt
    11761 tagaccatat ctaaaaaata ttgctgtagt tagagaactt aaagagtatt tgatagctag
    11821 tacagcattt attgttttgg atactttact taacgaaaca tatttgaaat attatttatt
    11881 atcagataat tttatcaatc gtgttaataa taaatctact ggaacaagtt atcctgcaat
    11941 caatgattat aattttaatc tattattaat t
//
LOCUS       DAVBCV010000062        11722 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBCV010000062 DAVBCV010000000
VERSION     DAVBCV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.062"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..199
                     /locus_tag="VQ516_001758"
     CDS             <1..199
                     /locus_tag="VQ516_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512156.1"
                     /translation="SKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKMFSTTYRNH
                     RKRFGLRMNLSAGIINHELGF"
     gene            349..603
                     /gene="blpM"
                     /locus_tag="VQ516_001759"
     CDS             349..603
                     /gene="blpM"
                     /locus_tag="VQ516_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET2512157.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            619..822
                     /gene="blpN"
                     /locus_tag="VQ516_001760"
     CDS             619..822
                     /gene="blpN"
                     /locus_tag="VQ516_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET2512158.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            1066..1215
                     /gene="blpO"
                     /locus_tag="VQ516_001761"
     CDS             1066..1215
                     /gene="blpO"
                     /locus_tag="VQ516_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET2512159.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1319..1438
                     /locus_tag="VQ516_001762"
     CDS             1319..1438
                     /locus_tag="VQ516_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2512160.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            2224..2622
                     /locus_tag="VQ516_001763"
     CDS             2224..2622
                     /locus_tag="VQ516_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2512161.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKKQTVFLILQQ"
     gene            2660..3349
                     /locus_tag="VQ516_001764"
     CDS             2660..3349
                     /locus_tag="VQ516_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2512162.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3391..3639
                     /locus_tag="VQ516_001765"
     CDS             3391..3639
                     /locus_tag="VQ516_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512163.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKDDA"
     gene            3669..4280
                     /locus_tag="VQ516_001766"
     CDS             3669..4280
                     /locus_tag="VQ516_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2512164.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4441..5235
                     /locus_tag="VQ516_001767"
     CDS             4441..5235
                     /locus_tag="VQ516_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2512165.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5232..5867
                     /gene="trmB"
                     /locus_tag="VQ516_001768"
     CDS             5232..5867
                     /gene="trmB"
                     /locus_tag="VQ516_001768"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2512166.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5993..6472
                     /gene="rimP"
                     /locus_tag="VQ516_001769"
     CDS             5993..6472
                     /gene="rimP"
                     /locus_tag="VQ516_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2512167.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6516..7652
                     /gene="nusA"
                     /locus_tag="VQ516_001770"
     CDS             6516..7652
                     /gene="nusA"
                     /locus_tag="VQ516_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2512168.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7674..7967
                     /locus_tag="VQ516_001771"
     CDS             7674..7967
                     /locus_tag="VQ516_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2512169.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7960..8259
                     /locus_tag="VQ516_001772"
     CDS             7960..8259
                     /locus_tag="VQ516_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2512170.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8276..11068
                     /gene="infB"
                     /locus_tag="VQ516_001773"
     CDS             8276..11068
                     /gene="infB"
                     /locus_tag="VQ516_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2512171.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11319..11669
                     /gene="rbfA"
                     /locus_tag="VQ516_001774"
     CDS             11319..11669
                     /gene="rbfA"
                     /locus_tag="VQ516_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2512172.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3590 a         2054 c         2662 g         3416 t
ORIGIN      
        1 cagcaaactc aaaccgctaa cagttgaaga taaagcctgt aaccatgcgc tatctaagga
       61 gagaagcaag gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt tttcaacaac
      121 ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgagtgctg gtattatcaa
      181 tcatgaacta ggattctagt tttgcaggaa gtctaatgta atttttgcaa ttcaagacgt
      241 ttcgatgact attcaagatc tggatgaaaa attttaaaaa acgatgatat actaaacttg
      301 tcaaagttac aacaagacaa aaattaaaaa taaaaaagag tatttgtcat ggatacaaaa
      361 attatggaac aatttcatga aatggatata actatgttat ctagtattga aggaggcaag
      421 aataattggc aaactaatgt cttggaaggt ggtggtgctg cttttggtgg ctgggggcta
      481 gggacagcta tttgtgctgc gagtggtgtt ggagcaccat ttatgggagc atgtggatac
      541 ataggagcta aatttggtgt ggatctttgg gcaggagtaa caggggcaac gggtggattt
      601 taataaaagg agacttgtat gaatacttat tgtaatataa atgaaacaat gctgagtgag
      661 gtttatggag gtaattccgg aggagcagct gtagttgctg ctttaggttg tgcagcgggt
      721 ggagtgaaat atgggagact tctaggacca tggggcgctg caataggagg aattggagga
      781 gcagtggttt gtggatattt agcctatacc gctacatcat aaaaatgaaa gctaaatttt
      841 atgaaggcta tattctttat tattctcttc gcttttcaaa cctatcttat ttatctatcc
      901 ataagtatct ccgataaaaa acaaaagact attgaattaa catcattgaa ttgttttgtc
      961 atattatttc taatctatga taaattaatt tttctcttta ttgcctatgt ttttttgata
     1021 atttttatat taaacttgtt ccgtaactga aaagtgatat actatatgga tacaaaaatg
     1081 atgtcacaat ttgcagttat ggataatgaa atgcttgctt gcgttgaagg tggagatatt
     1141 gattggggaa gaaaaattag ttgtgcagca ggggttgcat atggcgcaat tgatgggtgt
     1201 gcaacaacgg tttgacattt ctattgggac catttgctat aggaataggt gtaactggtg
     1261 ctgcaggtgg agctatactt ggaggtgtag cctatgcagc aatatgttgg tggtaattat
     1321 ggattttaaa agttttatta ttggtttagt agttggtata tttggtcctt atatggatga
     1381 cttaattaga aaaatatttt caaaatcttc caaaaaggat acggatagta ctctctaaaa
     1441 aataaatttc tcccctgtat ttgctagctt aatttacagg ggagtttctt taatattgtt
     1501 tcgcctgtca aaatgatatg tgatagcagg atgaaaagtc tacaaacaag ttggtatcaa
     1561 tacaatataa agaggaaagt atgtttttaa tgcttacttt ttaaattttt taacaattca
     1621 agatgtttcg atgacaattc aagatttgga tgaaaaattt taaaaaacaa tgatatacta
     1681 aacttgtcaa agttgcaacg ggtgctgcta cattatgtta aaaatcgctc ttgattgata
     1741 agctggattt gttaggaaag gagtaaataa ttatggataa gaaaaagata gtttcgacta
     1801 taatatgtat agtatttctt gtagtgtcag tagataattt ttttagagac ttaaccccgt
     1861 tactgttcat tttaaatatt attggcctgt cttgcttttc agtgctaaca tacattaaca
     1921 taaaagagat actcttaaat ataagtaaat gatgatttgg ttagtcctag aaccaatcaa
     1981 cttatgttta tcggcttggc agattttatg tttgtcattt gtttgtatag aggtatttca
     2041 gaaacagaat tctatcaaca actaatcgct tatataggtg tattctctgc ttgtttatca
     2101 cgcttctgct catgtggggc ttgaagtatt ataaaaagta gctttctatc cttttctgac
     2161 aggatatttg agtagaaggc tctattaaat tgttagtgaa ataaatgaat ggaggtattt
     2221 aatatgaaat acagattatt ttttgttatt ttcttgagta gtgtgttgga tattctttta
     2281 gggacatttt tacaaatctc tatcgtatct attgggtggc ttgttcttta cagtggtttg
     2341 tttgaagtag gagttttcct tcttgctaat aaaggggtgg cggtaaaaat caaggaagta
     2401 gatattcgaa atcgctttaa atttattttt ggaaaaacct tatggtttca aattcttttg
     2461 ctcatctttt tgataatcaa actttatctt ggtttggatg cgaggttgat tttattctat
     2521 ggacatattt tcattgtctt taatgcctta atgtatctat tatctagtag tcaggttagc
     2581 cttaaaaaaa aacaaactgt cttcttaatc ttacagcagt agagtctgac aaggtgaggt
     2641 catagtaggg aggatttcta tgaaaaagta tcaacttcta ttcaaaataa gtgcagtctt
     2701 ctcttactta tttttcgtat ttggtctttc tcagctgacg cttatcgtcc aaaattattg
     2761 gcaattttct tcccagattg gtaatttcgt ctggattcaa aatatcttga gtttactttt
     2821 tattggagtc atgattgtgg ttcttgttaa gacaggccat ggttatctct tccgcattcc
     2881 aagaaaaaaa tggctttggt attcgatttt gacagtatta gtggtagtgt tccagatctc
     2941 ttttaacgtt cagacagcta aacatgttca gtcaactgcg gaaggttggg ctgtattgat
     3001 tggttatagt gggactaact ttgcagagct aggtatttat atagccctgt tctttctggt
     3061 tccactgatg gaagaattga tttatagagg attactgcaa catgctttct ttaagcattc
     3121 gcgatttggt cttgatttgc ttcttccttc tattttattt gctctccctc atttttcaag
     3181 cctgcctagt ctgttagata tcttcgtctt tgcaacagtt ggaatcatct ttgctggttt
     3241 gacccgctat accaagagca tttatccatc ctatgcggtg catgtgatca ataatattgt
     3301 agcgaccttc ccgtttttgc tcacttttct acatagggtc ttggggtaaa aaaacaaaag
     3361 gcttgctttt cagccataga ggaggtcatc atgtataaac acttattttt cctagattcc
     3421 aaaactttag atcggttgac accctatatt ctagtcttgg cttctgacac cattgctttt
     3481 aatgtttttg tgctaacctt tgtatctgcg gtggttttta atttcctaaa ttccatgcta
     3541 gctttaatgg ctatattcat aggggctggc tatgtggtcg gattttggtt gctaaaatgg
     3601 tttgttttgg aaaggttaga gctaaaggat gacgcgtagg aagtctgttt ggtgaggagg
     3661 atagttttat ggagtttttt gataaatttc atgccttgtg ttttggattt ttagtactaa
     3721 taattgtcat tacagttcct tatacgatta accatggggg tttttttcaa aatgaatctg
     3781 cattgattct tgtaagtctt cttgtaacct cgctgagtgt tgcttatgct agaaagtttg
     3841 aaatgatttc ttttgggatg ttaagcaaga aacaactttt gcttttcatt gcaatctttc
     3901 ttctaagtgt acttgagacg ctggtttata ttcatttctt cgctgtttct tctggctcag
     3961 gggtccaaca cttggcggaa gtcagcagag gaatttccct gtctttgatt ttgactacct
     4021 cagtttttgg ccccatccag gaggaactca ttttcagagg acttcttcaa ggtgcggttt
     4081 ttgacaattc ttggttaggg cttgtgctaa cttcctctct cttttctttc atgcatggac
     4141 cttctaatgt cccttcgttt attttttatc tacttggggg tttgttgctg ggctttgctt
     4201 ataaaaagag tcaaaaccta tgggtttcta ctctagtaca catgttttac aacagttggc
     4261 cactcctata ttatttataa aaattatgaa aaggtaagca gaagacggtg cttacctttt
     4321 tcaagctata atgataccca agccaatcca gaggcttttc tttgagaatc gggttagagt
     4381 ggttgacgaa taggccaaaa actagtagaa tagtaaggaa actttatacg gaggaaagaa
     4441 atggatttgg gtgataatga gctaacactg actcccatac ctgggaaaag tggcaaggct
     4501 tatatgggta gctatcctga cgggaagcgc atctttgtaa aaatgaacac ctctccaatc
     4561 ctacctggtc tagctagaga acaaattgct ccacaattat tatggagtcg tcgtttggca
     4621 gatgggcgtg atatgtgtgc tcaagaatgg ttgacaggca agatattgac cccctatgat
     4681 atgaatcgta agcaaatcgt caatatttta acccgtcttc atcgctcacg tccgttgatg
     4741 acacaattga gtcgtttggg ctatgccatg gaaacacctg tagatttact acagtcttgg
     4801 caggaaacgg ctccagatgc tttgcgtaaa aatcatttta tcagtgaagt gatggctgat
     4861 ttacgtcaga ctattccagg atttagagag gaccatgcga ccattgtcca tggagatgta
     4921 cgacatagta attggattga gacagatagt ggcttgattt atttagtaga ttgggattcg
     4981 gttcgcttga ccgatcgcat gtttgatgtg gcccatatgc tctgccatta tatttcagaa
     5041 catcagtgga aggaatggtt gacctactac ggttacaagt acaatcaaac ggtattaagt
     5101 aaattgtatt ggtatggtca attgtcttat ttgagtcaga tttccaagta ttatatgaac
     5161 caagatttag aaaatgtcaa tcgggagatt catggtttgc gtcatttccg agacaagtat
     5221 ggaaagagaa gatgagagtt agaaatcgta aaggggcaac agaattacta gaggcaaatc
     5281 cccagtatgt ggtcctcaat cccttggaag ccaaggcaaa atggcgggac ttgtttggca
     5341 atgataatcc cattcatgtg gaagttggaa gtggaaaggg tgcctttgtt tcaggtatgg
     5401 ccaagcaaaa ccctgacatc aactatatcg ggattgatat tcaaaagtct gttttgagct
     5461 acgctttgga caaggtgctt gaagttggag tgcctaacat caagctcttg tgggtagatg
     5521 gttctgactt aactgactat tttgaagacg gtgagattga tcgcttgtat ctgaactttt
     5581 cagatccatg gccgaaaaaa cgccatgaaa agcgtcgttt gacctacaag accttcttgg
     5641 ataccttcaa acgtatcttg cctgaaaatg gagaaattca tttcaagacg gataaccgtg
     5701 gcttgtttga gtacagttta gtgagctttt ctcaatatgg catgaaactc aatggtgtct
     5761 ggttagattt gcatgccagt gattttgaag gcaatgtcat gacagaatac gagcaaaaat
     5821 tctcaaacaa ggggcaagtt atctaccgag ttgaggcaga attttaagag atagcctaaa
     5881 attaggctgt acaagtgctt ttgctttaca taagttggca aacgtgctat actgatagta
     5941 agaatatgaa aagtgaggcg gggaaatatc ttcgcctctt gcttatgagg aggtggacgc
     6001 aatcgcaaca atcgtagaat tagtcagaga agttgtagaa cctgtcatag aagctccttt
     6061 tgaactcgtg gatatcgagt atggaaagat tggcagtgac atgattctca gtatttttgt
     6121 agataaaccc gaaggaatta ccttgaacga cacggcagac ttgacagaaa ttatcagtcc
     6181 tgtcctagac accatcaagc cagatccctt cccagaacaa tatttcctag aaattaccag
     6241 tccaggtttg gaacgccctt tgaaaaccaa ggatgccgtc gctggagcgg ttggaaaata
     6301 catccatgtc gggctctacc aagccatcga taagcaaaag gtctttgaag gaaccttgtt
     6361 ggccttcgaa gaggacgagt tgactatgga atatatggac aagacgcgta agaaaaccgt
     6421 ccaaattcca tacagtttag tatcaaaagc acgtttagca gttaaattat agaaaaagaa
     6481 aggatagctt ttgaggattc aaaagtgaag aaaacatgag taaagaaatg ctagaggcct
     6541 tccgcatttt ggaagaagac aagggaatca aaaaagaaga tatcatcgac gcagtagtag
     6601 agtcgcttcg ttccgcttat cgcagacgct atggtcagtc agacagcgta gctattgact
     6661 tcaacgaaaa aacaggtgac tttacagttt atactgtccg tgaagttgtt gatgaagtat
     6721 ttgatagccg tttggaaatc agcttgaaag atgctcttgc cattaattca gcttatgaac
     6781 ttggagacaa aatcaagttt gaagaagcac cagctgagtt tggtcgtgta gcagcccagt
     6841 ctgccaaaca aaccatcatg gaaaaaatgc gcaagcaaac acgtgccatc acttacaata
     6901 cttacaaaga acatgagcaa gaaatcatgt ctggtacagt agaacgcttt gacaaccgct
     6961 ttatctatgt caaccttggt agcatcgaag cccaattgtc aaaacaagac caaattcctg
     7021 gagaagtttt tgcttctcat gatcgtatcg aagtttatgt ttacaaggtt gaagacaacc
     7081 ctcgtggtgt gaacgtcttt gttagccgta gtcatccaga aatgatcaaa cgtttaatgg
     7141 agcaagaaat tccagaagtt tatgatggaa ctgttgaaat catgagcgtg gctcgtgaag
     7201 caggtgaccg tacgaaggtt gctgttcgta gccacaatcc aaacgtggat gctatcggta
     7261 caatcgttgg acgtggtggt gctaatatca agaagattac tagcaaattc cacccagctc
     7321 gttacgatgc taaaaatgac cgcatggtac caatcgaaga aaatatcgat gttatcgagt
     7381 gggtagcaga tccagctgaa tttatctaca atgccatcgc tcctgctgag gttgaccaag
     7441 ttatctttga tgaaaacgac agcaaacgtg ccttggtggt tgttccagat aacaagcttt
     7501 ctcttgccat tggtcgtcgt ggacaaaacg tgcgcttggc ggctcacttg actggttacc
     7561 gtatcgatat caagtctgct agcgaatttg aagccatgga agacgctgct tcagtagagt
     7621 tggaagtaga aaacgatact gtagaagaat aaaagctgct agaggaggga aagatgaaaa
     7681 caagaaaaat ccctttgcgc aagtctgttg tgtctaacga agtcattgat aagcgtgatt
     7741 tgctccgcat tgtcaagaac aaggaaggac aagtctttat tgatcctacg ggcaaggcca
     7801 atggccgcgg cgcttatatc aaactagaca atgcagaagc cctagaggcg aaaaagaaga
     7861 aggtctttaa ccgcagcttt agcatggaag tggaagaaag cttttatgac gagttgatcg
     7921 cttatgtgga tcacaaagtg aaaagaagag agttgggact tgaataagca aaagataagt
     7981 aatctcttgg ggcttgctca gcgagcaggg cgcatcatat cgggtgaaga attggtggtc
     8041 aaggccattc aagacggcaa ggccaagttg gtctttctag ctcatgatgc tggacccaat
     8101 ctgaccaaga agattcaaga taaaagtcat tattatcaag tagaaattgt aaccgtgttt
     8161 tcaacactgg aattaagcat agcagtcggg aaatcgagaa aggttttggc tgtaacagat
     8221 gctggattta caaagaaaat gaggtctctt atggaataga agaggaggac atgatttgtc
     8281 taagaaaaga ttgtacgaaa tcgcaaaaga acttggaaaa gaaagtaaag aagttgtagc
     8341 gcgtgcaaaa gagttgggct tggatgtgaa aagccactca tcaagtgtgg aagaagctgt
     8401 cgctgcaaaa attgctgcca gctttaagcc tgcagctgct ccgaaagtag aagcaaaacc
     8461 tgcagcacca aaagtaagtg cagaaaagaa agccgaaaaa tctgagccag ctaaaccagc
     8521 tgtagctaag gaagaggcaa aaccggctga gccagttgct ccgaaaacag aaaaagtagc
     8581 agtgaaaccg caaagtcgta atttcaaggc tgagcgtgaa gcccgtgcta aagagcaggc
     8641 agaacgacgc aagcaaaata agggcaataa ccgtgaccaa caacaaaacg gaaaccgtca
     8701 gaaaaacgac ggccgtaatg gtggaaaaca aggtcaaagc aaccgcgaca atcgtcgctt
     8761 taatgaccaa gctaagaagc agcaaggtca gcaaaaacgt agaaatgagc gccgtcagca
     8821 agaggataaa cgttcaaatc aagcggctcc acgtattgac tttaaagccc gtgcagcagc
     8881 cctaaaagca gagcaaaatg cagagtacgc tcgttcaagt gaggaacgct tcaagcagta
     8941 tcaggctgct aaagaagcct tggctcaagc taacaaacgc aaggaaccag aggaaatctt
     9001 tgaagaagcg gctaagttag ctgaacaagc acagcaagtt caagcagtgg ttgaagtcgt
     9061 ccctgagaaa aaagaacctg cagtggatac acgtcgtaaa aaacaagctc gaccagacaa
     9121 aaatcgtgac gattatgatc atgaagaaga tggtcctaga aaacaacaaa agaatcgaag
     9181 tagtcaaaat caagtgagaa atcaaaagaa tagtaactgg aataacaaca aaaagaacaa
     9241 aaaaggcaat aacaagaaca accgtaatca gactccaaaa cctgttacgg agcgtaaatt
     9301 ccatgaattg ccaacagaat ttgaatatac agatggtatg accgttgcgg aaatcgcaaa
     9361 acgtatcaaa cgtgaaccag ctgaaatcgt taagaaactt ttcatgatgg gtgtcatggc
     9421 cacacaaaac caatccttgg atggggaaac aattgaactc ctcatggtgg attacggtat
     9481 cgaagccaaa caaaaggttg aagtggataa tgctgacatc gaacgtttct ttgtcgaaga
     9541 tggttatctc aatgaagatg aattggttga gcgtccacca gttgttacta tcatgggaca
     9601 cgttgaccac ggtaaaacaa cccttttgga tactcttcgt aactcacgtg ttgcgacagg
     9661 tgaagcaggt ggtattactc agcatatcgg tgcctaccaa atcgtggaaa atggtaagaa
     9721 gattaccttc cttgatacac caggacacgc ggcctttaca tcaatgcgtg cgcgtggtgc
     9781 ttctgttacc gatattacga tcttggtcgt agcggcagat gacggggtta tgcctcagac
     9841 tattgaagcc atcaaccact caaaagcagc taacgttcca atcatcgtag ccattaacaa
     9901 gattgataaa ccaggtgcta acccagaacg cgttatcggt gaattggcag agcatggtgt
     9961 gatgtcaacc gcttggggtg gagattctga atttgttgaa atctcagcta aattcaacca
    10021 aaatatcgaa gaattgttgg aaacagtcct tcttgtagct gaaatccaag aactcaaggc
    10081 agacccaaca gtgcgtgcaa tcggtacggt tatcgaagcg cgcttggata aaggaaaagg
    10141 tgcggtcgca acccttcttg tacaacaagg taccttgaat gttcaagacc caatcgttgt
    10201 cggaaatacc ttcggtcgtg tccgtgctat gaccaacgac cttggtcgtc gtgttaaagt
    10261 tgctggacca tcaacaccag tctctatcac aggtttgaac gaagcaccga tggcgggtga
    10321 ccactttgcc gtttacgagg atgaaaaatc tgcgcgtgca gcaggtgaag agcgtgccaa
    10381 acgtgccctc atgaaacaac gtcaagctac ccaacgtgtt agccttgaaa acctctttga
    10441 tacccttaaa gctggggaac tcaaatctgt taatgttatc atcaaggctg atgtacaagg
    10501 ttctgttgaa gccctttctg cctcacttca aaagattgac gtggaaggtg tcaaagtgac
    10561 tatcgtccac tcagcggtcg gtgctatcaa cgaatcagac gtgacccttg ccgaagcttc
    10621 aaatgccttt atcgttggtt tcaacgtacg ccctacacca caagctcgtc aacaagcaga
    10681 agctgacgat gtggaaatcc gtcttcacag cattatctac aaggttatcg aagagatgga
    10741 agaagctatg aaagggatgc ttgatccaga atttgaagaa aaagttattg gtgaagcggt
    10801 tatccgtgaa accttcaagg tgtctaaagt gggaactatc ggtggattta tggttatcaa
    10861 cggtaaggtt gcccgtgact ctaaagtccg tgttatccgt gatggtgtcg ttatctatga
    10921 tggtgaactc gcaagcttga aacactataa agacgacgtt aaggaagtta caaacggccg
    10981 tgaaggtgga ttgatgatcg acggctacaa tgatattaag atggatgatg tgattgaggc
    11041 gtatgtcatg gaagaaatca agagataaga ttttttgctc ctttcttagg tggtgaggga
    11101 cgcaagcaaa ccgatggttt cattgcttat ttttgagcct agggtctcaa aaatcccctg
    11161 tgatgggact gataaatcag ttccatcact ttcaccacgg cgaaagaagc agatgacttc
    11221 aaattgaact tcgtttcaat ttaaactgaa aatcaagaag tttaaaatag ctaggtctgc
    11281 tggcctagct tttggttcaa agtagagaaa ggaatatcat ggcaaatcat ttccgtacag
    11341 atcgtgtggg catggaaatc aagcgtgaag tcaatgagat tttgcaaaag aaagtccgtg
    11401 atccacgtgt ccaaggtgtg accatcacag atgttcagat gctgggtgac ttgtctgttg
    11461 ccaaggttta ttacaccatt ttgagtaacc ttgcttcgga taaccaaaaa gcccaaatcg
    11521 ggcttgaaaa agcaactggt accatcaaac gtgaacttgg tcgcaatttg aaattgtaca
    11581 aaatcccaga tttgaccttc gtcaaagacg agtccatcga gtatggaaac aagattgacg
    11641 agatgctacg caatctggat aagaactaaa gaagaggggt tgcccctctt tttcggctct
    11701 ttgtcaactg tagtgggttg aa
//
LOCUS       DAVBCV010000063        11615 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBCV010000063 DAVBCV010000000
VERSION     DAVBCV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.063"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            1..1046
                     /locus_tag="VQ516_001775"
                     /pseudo
     CDS             1..1046
                     /locus_tag="VQ516_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1162..1965)
                     /locus_tag="VQ516_001776"
     CDS             complement(1162..1965)
                     /locus_tag="VQ516_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2512173.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(2244..2999)
                     /locus_tag="VQ516_001777"
     CDS             complement(2244..2999)
                     /locus_tag="VQ516_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2512174.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(3069..5720)
                     /locus_tag="VQ516_001778"
     CDS             complement(3069..5720)
                     /locus_tag="VQ516_001778"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2512175.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(5713..6291)
                     /locus_tag="VQ516_001779"
     CDS             complement(5713..6291)
                     /locus_tag="VQ516_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2512176.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(6291..6761)
                     /locus_tag="VQ516_001780"
     CDS             complement(6291..6761)
                     /locus_tag="VQ516_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2512177.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(6748..7326)
                     /locus_tag="VQ516_001781"
     CDS             complement(6748..7326)
                     /locus_tag="VQ516_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET2512178.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(7609..8004)
                     /locus_tag="VQ516_001782"
     CDS             complement(7609..8004)
                     /locus_tag="VQ516_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2512179.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(8100..8354)
                     /locus_tag="VQ516_001783"
     CDS             complement(8100..8354)
                     /locus_tag="VQ516_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2512180.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(8363..9697)
                     /locus_tag="VQ516_001784"
     CDS             complement(8363..9697)
                     /locus_tag="VQ516_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2512181.1"
                     /translation="MTDEWIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9697..9930)
                     /locus_tag="VQ516_001785"
                     /pseudo
     CDS             complement(9697..9930)
                     /locus_tag="VQ516_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
     gene            9961..10035
                     /locus_tag="VQ516_001786"
                     /pseudo
     CDS             9961..10035
                     /locus_tag="VQ516_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10024..10149
                     /locus_tag="VQ516_001787"
     CDS             10024..10149
                     /locus_tag="VQ516_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HET2512182.1"
                     /translation="MDRTDEVSNHTYSKVKLTWFEEIFEEYKMILLLILYHMERD"
     gene            complement(10199..10339)
                     /locus_tag="VQ516_001788"
     CDS             complement(10199..10339)
                     /locus_tag="VQ516_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512183.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(10336..10518)
                     /locus_tag="VQ516_001789"
     CDS             complement(10336..10518)
                     /locus_tag="VQ516_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512184.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(10520..11303)
                     /locus_tag="VQ516_001790"
                     /pseudo
     CDS             complement(10520..11303)
                     /locus_tag="VQ516_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3451 a         2529 c         2159 g         3476 t
ORIGIN      
        1 atgaaggctg gactaaagat agagtttctc ttgaatactg cctcccaagt cgtacgatac
       61 ttcttaactg gctagcacaa tacaggaaaa acgggtatac tattgttgag aaaacaagag
      121 agagagtacc tgagagcgga gaatgccatc ctaaaaaagt taagagaact ccgattgaag
      181 gaggaaaaag agaaagaaga aagacagaaa ttgttcaaga attaatgact gagttttcgt
      241 tagatcttct tctaaaagcc attaaactag ctcgttggac ctactactat cacttgaaac
      301 agctagacaa aacagataag gaccaagagc ttaaagctga aattcaatcc atttttatcg
      361 aacacaaggg aaattatgct tatcgctgtg ttcatttaga actaagaaat cgtggttatc
      421 tggtaaatca taaaagagtt caaggcttga tgaaagtact caatttacaa gctaaaatgc
      481 gacagaaacg aaaataaagg agacgttggt aagaaggcag agaatctcat tcaaggccaa
      541 tttgaaggat ctaaaacaat ggaaaagtgc tacacagatg tgactgaatt tgccattcca
      601 gcaagtactc aaaagcttta cttatcacca gttttagatg gctttaacag cgaaattatc
      661 gcctataatc tttcaacttc acccaactta gaacaagtac aaacaatgtt ggaacaggca
      721 ttcacagaga agcactacga gaatacgatt ctccatagtg accaaggctg gcaataccga
      781 cacgattctt atcatcggtt cctaagagta agggaattca agcatctatg tcacgcaagg
      841 gaaacagcca agacaacggt atgatggagt ccttctttgg gattctgaaa tcggaaatgt
      901 tttacggtta tgagaagtcg tttcagtcgc ttaagcaatt ggaacaagcc attatagact
      961 atattgatta ctacaacaat aaacgaatta aggtaaaact aaaaggactt agccttgtgc
     1021 aatacagaac taaatccttc ggataaatta tttgtctaac tttttggggt cagtacacct
     1081 aaaactttga tgatatacgt tttcttgtga gaatatttac ttcatttttg cctaaaattc
     1141 aatgtttact cagtatttgg attatgaaaa atcgaggtct aaatctagat acattttttc
     1201 tgaagacaaa tcattttgac caccgagcaa gagattttca aaaaaagctg ttaaaaactc
     1261 agaacgtcgc tgtaaaatct ttgcattatc taataccaag gcatcacgaa aatatttgga
     1321 atgttgctga aatggtgtat tatcaatatc aaaaccaaac tcacgaagat actgaatcaa
     1381 aaagaccgtt actgtccgag tgtttccttc gcgaaatgga tgaatctgcc agattcctga
     1441 aataaaatgc tggatttgtt taaccacatc cgcctgagtt agtgtcgcat atgcaacttg
     1501 tttttcctga ttaaaatcat aatctaaggt catttgaatc atggagtaat cagagtacac
     1561 aacactttca ccattcaaaa caggttcatt ctttgtgata ttggtctgac gaaattgccc
     1621 caccggaata gagggttcaa atatatcttg aaacaactcc ttatgaatag caagtaaggt
     1681 cgcaggacta aagctaaagc ctcttcgaga caatagttct acaatacgta gagaaaccaa
     1741 gtctgcctct tccgtacttg catcaatagt atggtgataa gccgttgcat cctcataaac
     1801 ctgctcataa gtcagttctc cccgggactg tttctcagcc aaagattcca tatacgctga
     1861 tggcactaga ttgtcaactt tctgcagacc aaaacctatc cgccataaat cacgcttcgc
     1921 ttcataagac aagtttggat tgtcaatgtt gtaagttgat tgcatagaaa tatcctttca
     1981 attgtttttc ttcattacca gtaaatcacg aaattggttg gaaactggct taaactataa
     2041 aaagtcctca caataggttc tatgatatat atatatatac ttcatgtaaa taataaaaga
     2101 gttataatca gcactactga taaattcaat accgatttcg ccatcttaaa acttagtcaa
     2161 acgaataccg ttgatttcca gttcattaag tcgttgacct gtcaagactt ctcgttcctt
     2221 caactggaaa agttaagggg gaactaatac aaatcctcta cgcttctata ttcaatgatt
     2281 tcatttttga taatattctt tttaaactca aaatgtttta aaggattatc gattaacact
     2341 aactttggaa tatcatctag atttgtgacc aaggataaaa taaaatccga ttgaaactcc
     2401 ttgacttttt caaattcttt tgcagttaaa cgcacacgcc ccttagattt tcggatacct
     2461 tttacttcgg caaggtaaga atactcttgg acatctacct gaaagtcata tccatcacca
     2521 taaagacggg catcggttaa ctgtccacct tggaattttt cttctttatc aaaatgaagg
     2581 ataaaataat tctcagcctc cattcccgtt tcctgtagac gtttgaattt agttcgaact
     2641 attggttttt caacgatggt tatgcctgtt ttcttccctt cactggcaag taacatttta
     2701 actatttcag cataactatg aacatcttca tttccaaaca tcatatcaat taaatcttta
     2761 cgaaaacgat aaacttcagc tttctgccac caaccttttc gattattgtc aaaataagga
     2821 tcaaataaat ccattctatt ttttacaact ccagttgttt cagcaattcc cagagaaacc
     2881 acatagcgat aaaactccga cttgctagag cattgaaatt cttttataaa aagcttgtca
     2941 aacttggcaa gaccgtagcc aattaaattt aacaattcat aatgagggtg tttggacatc
     3001 actttctcca tcaatagata ttgatttcat tataccaaaa aacacggctc atcaccgtgt
     3061 ttctgtgttt atttcaccaa cttcttcatc tcatcaatac gtgctacggt cgcatcatac
     3121 ttagcttggt aatctgcttg cttgtcgcgt tctttttgga caacttctgg tttagcgttg
     3181 gcgacgaagc gttcgttgct gagttttttg ccaaccatgt cgagttcttt ctgccatttg
     3241 gcaagttctt tttccagacg tgccaattct tcttcgacat tcaagaggtc taccagtggc
     3301 aagaagattt cggctcctgt gatgacgctt gacatagcca gttcaggtgc agggatggtt
     3361 gatgcgattt ccaagtgttc aggatttgtg aaacgtttga tgtagttgac attgctgtta
     3421 aagaaggctt ccaagtcgct atcgcttgtc ttaacaagga tagtgatagg cttgcttgga
     3481 gcaacgttta cttccgcacg cgcattacga acggcacgga tcaagtcttt aaggctttcc
     3541 acaccagtgt gagcagcaag gtcttcaaag gctgggttga cagttgggta ttctgctgta
     3601 acgatagatc cttctgagat ttgtccaaag atttcctctg tcacaaatgg catgattggg
     3661 tgaaggagac gaaggatctt gtccaaagtg tatagtagaa cagaacgtgt gatgactttc
     3721 tcttcttcgt tatcgctata aaggacttcc ttggtcaact caacatacca gttggcaaat
     3781 tcttcccaga tgaagttgta aaggatatga ccagccacac caaactcgaa cttatcaaag
     3841 ttttcagtaa cttttgcaat ggtttcgttg agattgtgga gaatccagcg gtccgtcaca
     3901 ttaccagcct cacctgttgc aacttttgtg acattgtcat gcgccacatc cagcgtcaaa
     3961 ccttcattgt tcatgaggat atagcgagaa atgttccaaa ttttgttaat aaagttccat
     4021 gaagcatcca ttttctcgta agagaaacga acgtcttgac caggagctga gccgtttgac
     4081 aagaaccaac gaagactatc tgtaccatat ttatcaataa catccattgg gtcaatgcca
     4141 tttccaagag atttagacat cttacgtcct tcttcatcac gaattagacc atggataaga
     4201 gcatttttaa atgggctctt tcctgtaaat tcaattcctt ggaaaatcat gcgtgatacc
     4261 cagaaaggaa taatatcata gcctgttacg agtgttgaat ttggatagta gcgtttaaag
     4321 tcagtagttt cggtattagg ccagcccatg gttgagaagg gccagagggc agagctgaac
     4381 caagtgtcaa ggacatcttc gtcctgtttc cagccatcgc ccgctggggc ttcctcgccg
     4441 acatagattt cgcctgcttc attataccaa gcagggattt ggtgacccca ccagagctga
     4501 cgggaaatca cccagtcgtg gacattttcc atccattgca ggaaggtgtc gttgaagcgc
     4561 ggtgggtaaa attccacctt gtcatctgtg tcttggttgg caatggcgtt cttggccaat
     4621 tggtccatct tgacgaacca ttgagtagac aagcgtggct caactacgac acctgtccgt
     4681 tctgagtgac cgactgaatg gacacgtttt tcgattttga caagggcacc gatttcttcc
     4741 aacttagcaa cgactgcctt acgagcttca aaacgatcca tgcctgaaaa ttcaaaggca
     4801 agctcgttca tagttccgtc gtcgttcatg acgttgactt gtggcaagtt atgacgttgg
     4861 ccaaccaaga agtcatttgg atcgtgggca ggtgtgattt tcacgacacc agtaccaaac
     4921 ttaggatctg cgtgctcatc accaacgatt gggattaatt tattagcaat tggaaggatg
     4981 acatttttac caatcaagtc cttgtagcgc gggtcttctg ggttgaccgc aaccgcaacg
     5041 tccccaaaca tggtctcagg acgagttgta gcaacttcaa gggcgcgtga accatcttcc
     5101 agcatgtaat tcatgtgata gaaggcacct tcgacgtcct tgtgaataac ctcgatatca
     5161 gaaagggctg tgcgagctgc tgggtcccag ttgatgataa actcaccacg gtagatccag
     5221 cctttcttgt aaaggtccac aaagacctta cgaacagctt ttgacaaacc ttcgtcaaga
     5281 gtgaaacgct cacgagagta gtctacagag agccccatct tgccccattg ttccttgata
     5341 gtagtggaat attcgtcttt ccattcccag actttcgtca agaaagactc acgaccaagg
     5401 tcatagcggg aaatgccctc accacgcaag cgctcctcta ccttagcctg agtggcaatc
     5461 cctgcgtggt ccatcccagg aagccaaagg gtatcaaaac cttgcatgcg tttttgacgg
     5521 atgataatat cttgcaaagt tgtatcccaa gcgtgaccaa ggtgaagttt acctgtaacg
     5581 tttggtggtg gaataacgat tgaataaggc ttagcctttt gatcgcctga aggcttgaaa
     5641 acatcagcat caagccattt ttggtaacga ccagcctcaa cctcggctgg attgtattta
     5701 gatgaaagtt ctttagacat gtgtgtgtcc tttctctatt ttgtttattt tattttgaat
     5761 ttgcttagca gcttcttctg cagacaaatt cgtattattt attttaaagt agtggtgcaa
     5821 ctcattcggt tgatattggg aatttaattg aagtgtttca gcggtctcta aaatttctct
     5881 ttcagatacc tcaatatgtc gttttaaggg tttgtgcttt aatcgattct ccgttcgatt
     5941 tcgacgtatg cactcttcaa gacttgtttc caactcaaca aaaagaatct cttgatgata
     6001 atcatccaac aaattctgaa tttgctttaa atacatcagt tgatactgat ttgaaaaatc
     6061 aattacatct gttaaaatta ctgatcgctg atttcttgca cttgctccaa ggaatgaaaa
     6121 agtgattcca cgaacaaatt cccacatttc ctctgtatag tcctgataga tttctagtgc
     6181 aaaatcaatg gcttgatggt tataaaatag ggtagcatcc gtcagtcgag ataattcttg
     6241 accaatggtc atttttcctg atgctggagc accaatgatg aaaagatgca tcaaatcacc
     6301 tcccactcac tcttcagcaa ggcatatctc aaatcatcac agcagttgcc ttgagcatct
     6361 ttacggtctc ttatgcgagc ttcgagggta aagccaagct tttccgcgac tcgttgactt
     6421 tgaaggttat atccaaagca agttagttca atcttgtgaa gacccaaatc tttaaaggct
     6481 aagtcaatca aggcacgcgc agcttctggc acataacctc gcccccaata gtctgggtgt
     6541 aaggtatagc cgatttccag cacatcatct tcatggcgat ggttgaaatc gacagagcca
     6601 acgatcttat ccgttccttt gacgacaatc ccgtagcctg ctggataggc gacctctggc
     6661 aaactagcat agtcaaagat atcctcagca tccgccacag tacggactcg taaaacgaga
     6721 cgttctgttt cgattttatc tggcagctca gttcttgcca tccttcttcc tcgctttttt
     6781 gatgaaactg cccttcatat ctacacgctt atccagatag cgataaacgc gctgatatcc
     6841 atctcccatg aaataggttg gggcaaacag ttgattttta aaatgtccct tttcatccag
     6901 gagttctggg gcaacaagtc gctcaagaat cttggcaaag atgtggcaaa taccgtcttc
     6961 ctcaacaatc ctatctaccc gacaatctaa aacaagtgga caggcgtcta aaataggagt
     7021 ctgagttcgt tcagaaattt cataatgcac tcccaaacgt tccaatttct cctgatgact
     7081 gataaaacca gcctgctcca tcgcaagcat agaagtttca tcagaaatat tcacagtaaa
     7141 tttttgatac tgtttgatct gctctgcggc attctctctc gcaacgactc caatcacaac
     7201 ccaatctcct agactatacg aggaactaca ggtcgtgatg ttatagccaa aattctgatc
     7261 ttgatatcct aaaataaaaa caggaaaacc ataatatagt ttattagttt taaatgattg
     7321 ttttataaca acctcctttg actaagacac aaaagaaaag ccctgccatc tacatgacag
     7381 ggacgaatgt atttatccgc ggtaccaccc aatttcgggc caagcccgca tctttagtct
     7441 ttaaaataaa aagacagaaa tatttcattt tttcagtcat tagcaaccag ttttgacaca
     7501 tttgtggact tctcagcgcc gccactttct gtaaaatgtg ggattcaaaa cacctctaat
     7561 agctctattg tagcaagttt ttctcggaaa tgcaaggctc aagaaaaatt acttgaactt
     7621 gatgccattt tctttcaatt tcttgatggt agctgggccg attcctttca aggcaaggag
     7681 ttctttttca gtccagtttt tgaaatctga agcagacttg attccttcat catagaaagt
     7741 cttggcacga tcaagtggaa gtccacccaa gtttgctgca aaatcttcta cagaattagc
     7801 tactttttga gcttgctctt tggtagcttc tactgcttgt tcaacttttt tagaaacagc
     7861 cttaccagct gcgcttgctg actgttctgc acgcttcacg actttttttg tctcatctac
     7921 aaccttagtc acagtacttg aaaaggcacc tgcgcgacgg aggccatttc gtaattgttt
     7981 tttacgattg agtttctttg acatgataaa atcctttcaa taaaaaatcc ctgctctgac
     8041 aaggatttaa cataaacatt ctatcaagat tttagtgaaa aatcaagaat ctttctcgtt
     8101 taataatttc gctcaccaaa caagccttct ggcaaatcat ggaatccctt gcctactttg
     8161 aagttttcaa acttaccaag caagaactga taagcatcca agcgactctc gtagcgaatc
     8221 atggcatctt tggcacgctc gtcctggtct tcttcgtaaa ctttttgact ttccttgtgc
     8281 gccatgtcgt taatcatgat ttgggtattg acccaagctt caaattcctt cataaattct
     8341 tgttcgtaac tcatttttct ccttattcta atccacgaaa atagtccgta tcaatctcac
     8401 ggtagggctg gatatcctga acatactcca acactccttg gaattctccg tcttcatcgt
     8461 gtactgcagc ataggtgatg tggacaaact tacctcgcga ctcagacttg aaccacattt
     8521 catacttgtc tttgcttccc tcacgaagcc tcttcatgat agttttgacc ttgtccaagt
     8581 acttaggcgg atggcagagt tcgacattgc gcccgacttg agacggcgtc cgtttgaaaa
     8641 tcatctcatc agctggcgta ttgtcattgt aatactggaa aatatcttct ttattgacaa
     8701 aggtaatctc catagggaga tgattgagga tgagattggc ctgctcgact gaaagatagc
     8761 cattaccaaa agcctgttga ctatggcggt ccagcacagc ttccttttcc ttaggggtaa
     8821 aggtaatggt aaaatggcct tctggcgtat ctatgacttg ttgaacttga ccttctgccg
     8881 tatctagctg tacaggctcc tctgcaatct tttcctcaat aaagctctgt cgttctggca
     8941 cccatttctc tgacggacgg atgatggcat agccataggc atcgctctcc tccgcaatct
     9001 gaagccagtc atcctgagta aaagactcaa ggagaatcat gaggaggatg gactcttcct
     9061 tgaaaatcat actttcaaac tctgtcgcaa aagcttcaaa agcttccttt acactgctaa
     9121 ttgacacttc tggtagtgac ttgactgtcg ttagagctgt ttgaaagagt tccctaatct
     9181 gatcatccac tccccacata actttgggag gtgaatcgtg tccatagcgc tccatgatag
     9241 gaaagaagag ttcttcctta cgttggtaat ggatgtcaaa ttgccccaca agccccatct
     9301 gacgcaccaa gcccttgcgc atctccgcaa gcatttcctc gtcttccatc aactcatagg
     9361 tatctaacaa tctacgaatg cgaatcaaag ctgcacggag ggccaaattt tcttccttaa
     9421 agacacgaac tgggtgccca gggtgttcgg tatctaaaac ttcgacacct ttgatagcat
     9481 ttttaaaaag attggcatgg acatcacaga gttccataac atcttcaaaa gtgacgcccg
     9541 aatccgagtt catcagctcg tgctccataa gggaaatctc gatggctgac acgcccgtaa
     9601 aggtcgcatc aaagcgatct tgaaccgact caggagaggc gccattgtgc aattctaaca
     9661 aaatatcccg taggatatga atccattcat ctgtcattag tctaatccaa tcacttcgta
     9721 gccattcgct tccagtgtgc gtacaatctt gtccatagga gttcctgtta gcttagaacc
     9781 ctgtttaagt gatactttac gaccaactgt attgcgcatt aagggattgg caaggggttt
     9841 aaaacccaac tccactagaa tttacaagac ttctggatgc ttgtccacca cttctgcaac
     9901 aggaattgac acatcgatga tattgtccat gacgacctcc attgtatgtt ctaatactca
     9961 atgaaaatca aagagcaaac taggaagcta gccgcaggtt gctcaaaaca gtgttttgag
    10021 gttgtggata gaactgacga agtcagtaac catacctaca gcaaggtgaa gctgacgtgg
    10081 tttgaagaga ttttcgaaga gtataaaatg attttactgc ttatattata ccatatggag
    10141 agagattaaa aaactagcag tggagttcct gctagttttc tgcgacttgg attaccatct
    10201 agataagttt aaagaaccaa tccaatatct tgtaaaacag caattgaact gcaaaagaaa
    10261 tgatcatcca aaagaatttc acaatcccaa taatcggttt gataaaaaga atagcaagaa
    10321 ttgtccagaa aaatttcatt ctctctcctc tggcttaatg agccaccgag ccgtatccat
    10381 tagcttatct gcaataaaga gttttcccag accaacaacg acatggtcat ctttctttat
    10441 cgttttcatc acttttacac cttgcacggt caaaaagtaa ttaccataaa ttttagcaaa
    10501 agctttgata ggattcatat tattctcctt tgatgatttc agcctctttt cggattgttt
    10561 caacatcttc ttgatattga acttcactat agttgactag cttggataat tctttctgca
    10621 agctttcccc cattggtttc atagttgcaa aggttaaacc acctgaaatg ataccaccca
    10681 agataggaat aaatttcccc attcctttgg ccaaccctcc cttggtaaga ttcacaccaa
    10741 atatttttaa gacttttttc aaaatagggt accaaagcgt ctttgttaaa gctttattag
    10801 gcactgtttt cattacctgt ttggcaattg ttataccacc agcacgtagc aaacaaagca
    10861 gcggttccat tcacccctaa cattacgcct agataaagca agagggtatt ttgagcatct
    10921 tcactcaact cctcacgtga agcccaaaga tcctcataac cataaatata acctaattct
    10981 tgggccaatt tcagagagaa accataaaat tgagccacat aagctggaat ggtaattgcc
    11041 atagcaagcc ctccaggtaa tcctgccaaa acagaagtcc cactcgctaa taaaacatta
    11101 tccctaatac aggcattagc cactcgatct aagatttctt gagataagag agacgttgga
    11161 ccttgttcca ataaggtagg aatgtcctgt ggacccaatt ctttggaaaa cttatccact
    11221 aaaaatttcg aacgatcaac cttaacaact ggtagtttta ccacttgttg taatacttgc
    11281 atagctaagt cttgttcagc catatacaaa ctccttattt cttttttgca ccttatcata
    11341 ttatatttcc tacaaaaaaa gcattttcta attagataac attaaaaaat acagaacccc
    11401 ttttttatag taaaataaaa caagaacagg acaaatcgat caggacagtc aaatcgattt
    11461 ctaacaatgt tttagaagta gaggcgtact attctagttt caatatattt taaaagaata
    11521 tttttccatt tcaaattcaa cctattttcc tccaccaaaa aagggctcta taatatttgt
    11581 agtgggtaaa ttccctatgg atattatgga gccta
//
LOCUS       DAVBCV010000064        10989 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBCV010000064 DAVBCV010000000
VERSION     DAVBCV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.064"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            136..2316
                     /locus_tag="VQ516_001791"
     CDS             136..2316
                     /locus_tag="VQ516_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2512185.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VQ516_001792"
     CDS             2368..3183
                     /locus_tag="VQ516_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2512186.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETLVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            3323..4666
                     /locus_tag="VQ516_001793"
     CDS             3323..4666
                     /locus_tag="VQ516_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2512187.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            5043..6233
                     /locus_tag="VQ516_001794"
     CDS             5043..6233
                     /locus_tag="VQ516_001794"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2512188.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6786..7721
                     /locus_tag="VQ516_001795"
     CDS             6786..7721
                     /locus_tag="VQ516_001795"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2512189.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7755..8792
                     /locus_tag="VQ516_001796"
     CDS             7755..8792
                     /locus_tag="VQ516_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2512190.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8965..9570
                     /locus_tag="VQ516_001797"
     CDS             8965..9570
                     /locus_tag="VQ516_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2512191.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9726..10256
                     /locus_tag="VQ516_001798"
     CDS             9726..10256
                     /locus_tag="VQ516_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2512192.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQ516_001799"
     CDS             10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQ516_001799"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2512193.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFP"
BASE COUNT         3341 a         2017 c         2395 g         3236 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaggtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatttttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 actagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agcgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgatgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtgatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaaaat
     1861 cttactgagg aagtttactc agtagcagac ggtcaagttg ttggtttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgtg tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttgc agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccacgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactttagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatgtt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attccagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 gatagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagata agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcagat atgaccttgg atatgggatt cttggaaatt gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat caaatcctgt tatggagaaa attaagacta aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccaattg actcagttga tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggagatatt gcccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttagcagc gcgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcaccgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagatgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa actaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgccaaaca ttttaataga aattgttgga
     4681 gtactgagct ccaacttttt tatttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtagtt tgcaattcgc aaagaatgac
     4861 ggcttcgagg tcgctcgtct ttctacggtt gccgccatac tgcggtcacc ctatgcgcct
     4921 tactagcttc agaaataaaa aagtatcgtt tttatttctg aagctagtaa ctcttagata
     4981 atggaaattc ctatcagtac tttgtaactc tataacaata ttttttaagg ggggacattt
     5041 ttatgtcaga acgtaaatta ttcacgtctg aatctgtatc tgaggggcat ccggataaga
     5101 ttgcagacca aatttcagat gcgattttgg atgctatttt agcaaaggat ccagaggcgc
     5161 acgttgctgc tgaaacagct gtatatactg gttctgtcca cgtttttggt gaaatttcta
     5221 caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac cattgcagag attggttata
     5281 ccaatacaga atatggattt tctgctgaga cggtgggagt acacccatct ttggtggaac
     5341 aatctcctga catcgctcaa ggtgttaacg aagccttgga ggttcgtgga aatgctgatc
     5401 aagatccact ggacttgatt ggagcaggtg accaagggct catgtttgga tttgcagtag
     5461 atgaaacaga agagctcatg ccattgccaa ttgcactcag tcataaattg gttcgtcgtc
     5521 tggcagaact tcgtaagtct ggagaaatta gctatctccg tccagatgca aaatcacaag
     5581 ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt agatacagtc gttatttcta
     5641 ctcagcatga tccagaggcc actaatgaac aaatccatca agatgtgatt gacaaggtca
     5701 tcaaagaagt tattccatct tcttatcttg atgataagac aaaattcttt atcaatccga
     5761 caggtcgttt tgtaatcggt ggtcctcaag gggactcagg tttgactggt cgtaagatta
     5821 ttgtagatac ttatggtggc tactctcgtc atggtggtgg tgccttctct ggtaaagatg
     5881 cgactaaggt ggatcgttca gcctcttatg cggctcgcta tattgccaag aatatcgttg
     5941 cagcaggcct tgctaagaag gcagaagtgc agttggccta tgctatcggt gttgcgcaac
     6001 ctgtttctgt tcgtatcgat actttcggta caggaacagt agctgaaagt caacttgaaa
     6061 aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat tatccaaatg ctggacctca
     6121 agcgtccaat ttaccgtcaa acatcggctt acggtcacat gggacgtaca gatattgatc
     6181 ttccatggga acgtttggat aaggtagatg ctttgaaaga agcagtaaaa taagatttta
     6241 agaggggaac gtcctctctt ttttatagtt tttaactata ctgggatact gttctgaaaa
     6301 tccattttgc gaaagtagag atttacatgt ataatggatt gaaactagaa tagtacacct
     6361 caacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttc tcctgttctt
     6421 gtttcatttt actatatttc tttaaaaatg ataaaggttg agatttctcc tcgtaataga
     6481 taatcatggg gatatttcaa tccaaagttt tattcgttat cacttgacta ttgcaaggtt
     6541 ttctagagca acagagtcat ggaatggact catggttgag atttctcctt gttgcttgga
     6601 cttcattcaa aagtctgtta cccaagcctt gttcaaactt ccaatacact agctgtttcc
     6661 atagcatgac ttctgtacta gactttcttt tccgaataaa tagatagaac cacagaatct
     6721 agtaaaccta gaattaaaat tatggtataa tattagcaat aaaagaaatc tggaggatta
     6781 gaatcatggt atcaacgaaa acacaaattg ctggttttga gtttgacaat tgcttgatga
     6841 atgcagcagg tgtggcttgt atgacgatag aggagttaga agaggtcaaa aactcagcgg
     6901 caggaacctt tgttactaag acagcgacct tggacttccg tcaggggaat cctgagccac
     6961 gctaccaaga tgttccactt ggttccatca actctatggg cttgccaaat aatggcttag
     7021 actattattt ggattatctt ttagatttgc aggaaaaaga gtcgaaccga actttcttct
     7081 tatctctggt cggcatgtct ccagaggaaa cccatactat tttgaaaaaa gtccaagaga
     7141 gtgattttcg tggtctgact gagctaaatc tttcctgtcc aaatgttcca ggtaaacctc
     7201 agattgccta tgattttgag acaacagacc ggattttggc agaagtgttt gcttacttca
     7261 ccaaacctct tggaattaaa ttgccacctt attttgatat tgttcacttt gaccaagcgg
     7321 cagctatttt caacaaatat ccgctcaagt ttgtcaactg tgttaactct atcggaaacg
     7381 gcctctatat agaagacgaa tctgtcgtta ttcggcctaa gaatggtttt ggtggaattg
     7441 gtggagaata catcaaaccg actgctctag ccaatgttca cgccttttat caacgtttaa
     7501 atcctcaaat ccaaattatc ggaacaggtg gcgttctgac tggtcgagat gcctttgaac
     7561 acatcctctg tggagcaagt atggtgcagg tgggaacgac ccttcacaaa gaaggcgtca
     7621 gtgcttttga ccgcattacc aatgaactga aagcaatcat ggtggaaaaa ggctacgaga
     7681 gcttagaaga tttccgtggg aaattgcgct atattgacta aattaaatcg aaaaatctga
     7741 agaaaggaga gacgatgcta gccattgaag aaagtcagaa gttgacttta tcaaatttac
     7801 cgagcctgag cctatttaca gggacagatc agggtcagtt tgaagtgatg aagagtcaag
     7861 tgttgaaaca gattgggtat gattctgctg acctcaactt tgcctacttt gatatgaaag
     7921 aagtagttta caaggatgtg gaactggagt tggtcagcct tcctttcttt gcggatgaga
     7981 aaatcgtgat attagattat tttatggata tcacgactgc taagaaacgc tttttgacag
     8041 atgatgagct taagtcattt gaggaatacc ttgacaattc ttctccaaca accaagttga
     8101 taatctttgc agaaggaaag ctggatagca aaagacggtt agtcaaatta cttaagcgtg
     8161 atgccaaggt cttcgatgca gtagaagtaa aagaacaaga attgcgccag tatttccaaa
     8221 agtggagtca gaaacaaggt ctgcagttta ccaatcattc ttttgaaaat ctcctcatca
     8281 agtcggggtt tcaatttagc gaaatccaga aaaatcttct ctttttacag tcctataagg
     8341 cgaattctgt tattgaggaa gaggatattg ttaacgcaat tcccaagacc ttgcaggaca
     8401 atatttttga tttaactcag tttattctga ctaaaaagat ggatcaggcg cgcgatttgg
     8461 tgagagactt gaccttgcaa ggggaagatg aaatcaaact gattgcagtc atgctgggac
     8521 aatttcggac ttttactcag gtgaagattt tggcggagtc tggccaaaca gaatcgcaga
     8581 ttgcaagtag tttaggtagt tatctgggac gtaacccaaa tccttatcaa atcaagtttg
     8641 cattaagaga ttcgagagga ctttctttga gctttctgaa gcaagctatt tcctatttga
     8701 ttgagacaga ctatcagatt aagacaggtc tttatgaaaa aggtttcctt tttgaaaagg
     8761 cactcttaca gattgctagt caggtcaatt gacatttgtt gaaactacaa cccgcgtcag
     8821 gattatcttg tcgtgggttt cttgctgatt tgcttatttt attagaaagt atgtcagtga
     8881 tttccttatc tataagcaaa aaacttgaaa aaagtgaatg tttgcgttat cattttcata
     8941 tagaaagaaa aagaggtatt acagatggct attatcttac cagaacttcc atatgcatac
     9001 gacgctttgg aaccatacat cgatgcggaa acaatgcatt tgcaccatga caaacaccat
     9061 caaacttatg tcaacaatgc caatgcagct ttagaaaaac accctgaaat cggtgaagac
     9121 cttgaagcct tgcttgctga tgtagaatct atcccagctg atatccgtca agcacttatc
     9181 aacaatggtg gcggacactt gaaccacgct cttttctggg aattgatgac tcccgagaaa
     9241 acagctcctt cagcagaact ggcagcagca atcgatgcaa catttggttc atttgaagaa
     9301 ttccaagcag ccttcactgc agcagcaaca actcgttttg gttcaggttg ggcatggttg
     9361 gttgtcaaca aagaagggaa acttgaagtg acttcaacag caaaccaaga cacaccaatc
     9421 tcagaaggta aaaaaccaat cttgggcttg gacgtttggg aacatgctta ctacgtgaaa
     9481 taccgcaacg tgcgtcctga ctacatcaaa gctttctttt cagtaatcaa ctggaataaa
     9541 gtagatgaat tgtacgcagc tgctaaataa tgatagttgg agggaagaat tgttcttctc
     9601 tttttaggtt ataagattct ggtctgacaa aatcgtcaga cttttttcat ttttatgaga
     9661 aacgtgctaa ctgctagaaa ttatggtaga ataaagtatt aagtaatcgt ggaaaaagga
     9721 tatctatgcg aaaagaaatt gcacctgaat tatacaacta taacaagttt cctggtccgg
     9781 agttccattt acacggggat aaggtcgaaa cggaagggat agctttttcc ttggttgaaa
     9841 atatcaagga tgcctttgat gtgacgactt ttaatcagcg tttttcagaa gtcttaacca
     9901 agtttgatta tatcgtgggg gactggagca acgaacagct tcgcctacga ggtttttaca
     9961 aggatgaccg aacagaagaa aaacttgaaa aaatcagtcg tttacaagac taccttttag
    10021 agtattgtag ttatggttgt gcctattttg tcttagaaaa tgaagcccct aagcgagcat
    10081 catttgacaa gaaaatgcgt aagacggaag aagaaacacc ttctagaaaa ggaaagaaac
    10141 cgactcaaac taaacgaaag tcgaatgcag ataagaaaaa tagacgtcgt cagaaagacc
    10201 agcattctca gaaagaggac aaggaacaac gtcattttgt cattcgtcag aagtgagtag
    10261 agaataagga gaagaaatga aaccctcaat tcatagtttg gcacatcaaa ccatgcagga
    10321 atgggtattg gagcagggag aaaagaaatt ccgtgcagat caaatctggg aatggctcta
    10381 ccgtaaacgt gtgcagtcat ttgaagaaat gaccaacctt tccaaggatt tgattgctaa
    10441 gctcaatgac cagtttgtgg tcaatccctt gaaacaaggt attgtgcaag agtctgctga
    10501 tggtacagtc aaatatctct ttgaattgcc tgacggtatg ttaattgaga ctgtactcat
    10561 gtgtcaacac tatggtttat ctgtctgtgt gaccactcag gtcggctgta atatcggttg
    10621 taccttctgt gcctctggtt tgattaagaa gcagcgtgac ctcaataacg gtgaaatcgt
    10681 agcgcaaatc atgctggttc agaaatactt tgatgagcgt ggtcaggatg aacgcatcag
    10741 ccatatcgtt gtcatgggaa tcggtgagcc ctttgataat tacaacaatg tcttgaattt
    10801 ctttcgtact atcaatgatg ataaaggaat ggctatcggt gctcgtcaca tcacggtttc
    10861 aatctcaggt ttggctcata aaattcgtga ctttgctgat gaaggggttc aggtcaatct
    10921 tgccgtgtct cttcacgcac ccaacaatga acttcgttca agtatcatga agattaaccg
    10981 tgcctttcc
//
LOCUS       DAVBCV010000065        10791 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBCV010000065 DAVBCV010000000
VERSION     DAVBCV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.065"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            135..425
                     /locus_tag="VQ516_001800"
     CDS             135..425
                     /locus_tag="VQ516_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512194.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKNAKNCVRLSQLSLNKR"
     gene            449..1165
                     /locus_tag="VQ516_001801"
     CDS             449..1165
                     /locus_tag="VQ516_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2512195.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1294..2109)
                     /locus_tag="VQ516_001802"
     CDS             complement(1294..2109)
                     /locus_tag="VQ516_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2512196.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2106..3011)
                     /locus_tag="VQ516_001803"
     CDS             complement(2106..3011)
                     /locus_tag="VQ516_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2512197.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            3409..5538
                     /locus_tag="VQ516_001804"
     CDS             3409..5538
                     /locus_tag="VQ516_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2512198.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            5525..5974
                     /locus_tag="VQ516_001805"
     CDS             5525..5974
                     /locus_tag="VQ516_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2512199.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            6033..6305
                     /locus_tag="VQ516_001806"
     CDS             6033..6305
                     /locus_tag="VQ516_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2512200.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(6659..6850)
                     /locus_tag="VQ516_001807"
     CDS             complement(6659..6850)
                     /locus_tag="VQ516_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512201.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            7278..8036
                     /locus_tag="VQ516_001808"
     CDS             7278..8036
                     /locus_tag="VQ516_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2512202.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            8038..10026
                     /locus_tag="VQ516_001809"
     CDS             8038..10026
                     /locus_tag="VQ516_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2512203.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(10068..10712)
                     /locus_tag="VQ516_001810"
     CDS             complement(10068..10712)
                     /locus_tag="VQ516_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2512204.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNEEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
BASE COUNT         3340 a         2019 c         2254 g         3178 t
ORIGIN      
        1 tataccatat tttgtttcgc aggaagtcta atgctaaaat cttatcagtg agaagatttc
       61 ttaaagttta ttctttgtag tttcatctgt attagtacat aatttaactt agagaaaata
      121 ggaggactaa tcgaatggaa ttaaaagatt ttacagaaaa agaacaggaa atgattaaga
      181 aaaggcttac aatgtctaat attagtgaca aagaaactac tgagaagatt ctggcgctag
      241 tcccacaaga cttgattaag cgaatcccgt tttttgtcag aaaacatgct acaacacgta
      301 cgattaaacg tatttcaatt gaataccctg aactctacgc tgtagctcaa acaagtggtg
      361 agattccaga aaaaaacgcg aagaattgcg tcagattatc acaactatct ttgaacaaaa
      421 gatgaataag catagtatta agtagagaat gaaaaaatat tttattggcg gtttgggaag
      481 caatgcctat catagcaagg attttcttca agaactagat tcgcaggtct attttctaaa
      541 tccatatgaa aagcatcttc gagatgaaac agaattgaaa tcatggttta aaaatgagat
      601 tgtagaggaa gaatctatct gtctgatagg tcattctatt ggaggcgatt tagctcgtta
      661 tttcgcatcg gaatttgaag aagtaaagaa actgattctt ttggatggtg gctatctaga
      721 tttagataag attttacctt tggatacaga gttagaggaa actaaaaatt atatcaaatc
      781 tcaaattatt tcggacttag atgttcttac ttctaaagaa aaatctgaag caaagcattg
      841 gtcagaaaat atggagaaag ctgtaagaca gtcctatcac tggaatgttg agtataatag
      901 atatgagttg gctataaatt atgaaaatat agaagcgata ctccgcctac ggagaaaaat
      961 acaagctttt aagagagaag tgggagatac cttgtttatt agtcctcgct atcctaatga
     1021 agctacatgg agagaggaag ccctaaaaga attgccagac tattttgata ctattttttt
     1081 agaaaacttt ggccatgagc tttatactca agcacctaaa gaaatcgcta gtctaatgaa
     1141 tgagtggctc gcttattttc tatgagctac ttgtattgct ggaaatggta taagatgaaa
     1201 atctacaagt cactaatttt catataacaa aaaacgaaca ggctcttttt ctgttattca
     1261 gaagagaact tgttcgtttt tatcttgatt ttcttaaaaa gctctatata catagggatt
     1321 ttctggttta tcgactttgg taaagctgtc gatttccaag gttggaaggt tttgtttgag
     1381 ttctttatgg tagtgattga ccagttttcc tttggctgtt atccaagtat tgttttcata
     1441 ctgccgggta ttgcccttgg tcagcaatcc atagacacca gaatctgcga tacagtggat
     1501 aatgccgaat cggaacagaa attgactatt ggcatgactg gggtcgttat agacaaagcc
     1561 tgtaaactgg attgtcttgc cctcaaactc atctggatag tcgtagatag cctccatgac
     1621 ttccatatag ttttcattag tgatctgaat actatcttgg gataagtatt tatccgccgc
     1681 cgttcgcatt tccttttcat aggctgattt tgaaaaataa gaactggtat ctggtttcaa
     1741 atattggctt gtcgtccctt cgcttgtctg aatggctaga tctgttcctt ccgatagggg
     1801 gaaatgataa cctttagcag aaacggtctg agaatccaag ctaacagttg ggaaagttaa
     1861 gccgatgaca agtggaatag ccagaagaga aatactcgtc atcttggcca atcggctgtt
     1921 cagatgactg tgggttttga cttgcttcat ccagatatac aattgaacaa ttgccaagat
     1981 aaaggaaagc accatggaga tataggccag ataggaatag tgcatgttga tgtactggtt
     2041 tagtttgccc gacagatgga ggtaaatagt cagttcaaaa tagccagcta aaactaaaaa
     2101 tcgaatcata ggatgactcc aatcaagaga gaatagacta agacgacaag agttacaatc
     2161 gttatgaagt gactgataaa tcgtgctttc aagtaatttt tcatcatgag aatatttttg
     2221 atatccagca ttggaccaat gacgagaaag gccagaactg gtgccaaacc gaaactcgag
     2281 agaagagaag cacctataaa ggcatccgcc tcactacaga gcgaaagaag aaaggccaaa
     2341 atcatcaaga gcaggatggc aaaaagagga gtcgcactga tagaggtcag aatccgagtc
     2401 ggaacgtaga cctgtattat agaagcaaag aggcagccaa ataccaaata acgccccgta
     2461 tcaaaaaatt catcaatggc ctgcacaaag acttgaaaaa ctttttttgc agaactcaag
     2521 taagaaaaat catgctcatg acaagccaga cgattttctt tctgaatcgg ttcttgccag
     2581 aaaaatccta gaaaaattcc tagtattaca gccacaagaa tggaacccag agctcgtaat
     2641 agggcgacat ggaaggagtt gccaaaggca gaataggtcg caaaaagaac aatgggattg
     2701 ataacaggtg ctgtcacaag aaaaggaacg gctgtgtaac tgggaacctt tttttccaga
     2761 aaacgattga tgatggggac gattccacat tcacaagaag ggaaaagcat accgacaaag
     2821 gtcccaaaaa agattctccc ccaacgattt cgagggagaa aatgataaac cttgtcaggt
     2881 gtgatataaa cttcaatcag ccctgagaca atgcttccta ttagaacgaa gggaagggct
     2941 tcaatgataa tggagagaaa aatggctcca gtttgaagta cactagatgg gagttgttga
     3001 aaagcagtca tctagttttt cttttctgct ttggcttttt tctctttgtc atcagggaaa
     3061 gtaacttgtt tcaaatcagg gagtttagca aactcttcaa acatcttgtc caagtcgtct
     3121 cgttttgtaa attttacagc cataggtgta cctcgattct aaagtctacc tctattatac
     3181 tattattttg cggaaaaggg agttttttaa tttgttttcg atagttgata cacttcgaaa
     3241 atctcttcaa accacgtcag cttcgcctta ccgtatatat gtgacttatc cacaacctca
     3301 aaacagtatt ttgagcagcc tgcggctagc ttcctagttt gctttttgat tttcattgag
     3361 tatgaaaagg attggagaaa gccttccagc ttgtggtata atagatttat ggataaaaaa
     3421 tatgaaaaaa tctctcagga tttgggagtg atgttaaagc aaattgatac cgttctaagt
     3481 ttgacagctg aaggggcgac tattcccttt atcgcgcgtt atcgcaagga catgactggt
     3541 agtctggatg aggtggcgat taaggctatt attgatttgg ataaaagtct gacaaatctc
     3601 aatgaccgta aggaagctgt cttagctaag attcaagaac aaggtaagtt gaccaaggaa
     3661 ttggaagaag ctatcttagt tgccgaaaaa ttagcagacg ttgaagaact ctatcttcct
     3721 tataaggaaa agcgtcgtac caaggcaacc attgcccgtg aagctggact ctttcctctt
     3781 gctcgtttga ttttgcagaa tatagttgac ttagagaaag aagctgaaaa gttcgtctgt
     3841 gaaggatttg cgactggcaa ggaagccttg accggtgcag ttgatatttt ggtcgaagcc
     3901 ttatcggaag atgtgacctt gcgttctatg acttatcagg aagtgctgag acactctaaa
     3961 cttacttctc aagccaagga tgaaagtctt gatgaaaagc aggtttttca gatttattat
     4021 gatttttcag agacagttgg aactatgcaa ggctatcgta ccttggctct caatcgtggg
     4081 gagaaacttg gtgtcttgaa gatcggtttt gaacatgcga cggaccgtat tcttgccttc
     4141 tttgctactc gtttcaaggt gaaaaatgct tatattgatg aagttgttca gcaatccgtt
     4201 aagaaaaagg tcttgcctgc tatcgagcgt cgtattcgga cagaattaac tgagaaagct
     4261 gaagaaggag ctatccaact tttttctgac aatctgcgca atctcctctt ggttgctcca
     4321 ctgaaagggc gcgtggttct tggatttgac ccagcctttc gtacaggtgc caagttagct
     4381 gtcgtggatg caacaggaaa aatgctgaca actcaggtta tttatcctgt taaaccagca
     4441 tcagctcgtc aaatcgaaga agccaagaaa gatttagcag atttaattgg tcaatacggt
     4501 gtagagatta ttgccattgg aaatggaacg gccagtcgtg aaagtgaagc ttttgtagcg
     4561 gaagttctga aagatttccc tgaagtcagc tatgttatcg ttaatgaaag tggtgcttct
     4621 gtctattctg ccagcgaact tgctcgtcag gagtttccag acttgaccgt tgaaaaacgc
     4681 tctgccattt ctatcgcccg tcgtttgcaa gatcctcttg cggaattggt caaaatcgat
     4741 cctaagtcaa ttggtgtcgg tcaataccaa cacgatgtca gtcagaagaa actatctgag
     4801 agtctggact ttgttgtcga tacagtggtt aaccaagttg gtgtcaatgt caatacagct
     4861 agcccagctc ttctttcaca cgtagctgga ctcaacaaaa ctatctctga aaatattgtc
     4921 aaataccgcg aggaagaagg aaaaatcact tcacgcgccc aaatcaagaa agttcctcgt
     4981 ctgggagcca aggcctttga gcaggctgct ggtttccttc gtatccctga aagtagcaat
     5041 atccttgata atacaggagt tcacccagag aattacactg cagttaagga actcttcaaa
     5101 cgtttagata ttaaagacct aaacgaagaa gcccaaagca aactcaagtc cctttcagtc
     5161 aaggaaatgg ctcaagagct agacctcggt ccagaaaccc ttaaagatat cattgcagat
     5221 cttctcaaac caggtcgaga tttccgtgat tcctttgacg cacctgtgct tcgccaagat
     5281 gtcctagata tcaaagacct agtggtaggt cagaagctag aaggtgttgt gcgtaacgtt
     5341 gttgacttcg gtgccttcgt tgatatcggg attcacgagg acggcttgat tcatatttct
     5401 catatgagtc gcaagtttat caaacaccct agtcaagtgg tgtcagttgg agatttggtg
     5461 accgtttggg tcaagaaaat tgatactgaa cgtgaaaaag ttaatctgtc gctcctcgct
     5521 ccaaatgaaa ctgactgatt atgtcaagca ggtttcacta gaagacttcg ggagagcttt
     5581 tatccatcat gtccagtgga ataggcgtct acgttcgaca ggtgggcgat ttttccccaa
     5641 agatgggcat ctggatttta atcccaaggt ttatcagaaa ctgggtatgg aggtttttag
     5701 gaaaattgtc cgacatgaac tctgtcatta tcacctttat ttccaaggga agggctatca
     5761 acacaaggat cgggatttta aggaactgtt gaaagcagtg gatggattaa ggtttgtacc
     5821 atccttgccc aatagtaact ctaaaccact caagctttat cgttgtcaat cctgccagca
     5881 aaggtatcag cgcaagcgta ggattgatac caaacgctat cgctgtggac tttgtcgagg
     5941 taaattgctt ctgataaatc agcctgagga ctgatgaaag ccgagcctcc ccgtgatata
     6001 ctatgtctaa cctagcagaa agaggaaata tcatgaacag taaattttat aaaatgagac
     6061 gcaatcgcat gatttcagga gttctggcag gtctgtcaga caagtggaat tttgatgtta
     6121 ccctagttcg tttcttgttt gccattttta cagttgcaaa ctttggactt ggcgtgatta
     6181 tctatatcat cctagcttct atcatgccta ccaaggaaga aatcgaagca gaaatgtacg
     6241 gaacaggccc acgtaaacgc aaagaagccc aagccattga cgacaatgaa ggctggtttt
     6301 ggtgaggttc tacagaagat ttggaataag caaaaagctt taaatcaata gtctaaactg
     6361 cttgatttaa agctttttta cttttatact caataaaaat caaagtgcaa actaggaagc
     6421 cagccacagg ctgtaattta gtcggcaatg tgaagttgac atagtttgca ctttgatttt
     6481 tgaatagtat taataatatt tcgccagctt catccacaaa ctatagtgga ttggaactag
     6541 aatagtacac ctctacttct aaaacattgt tagaaatcga tttgactatc ctgatcgatt
     6601 tgtcctgttc ttatttcatt ttattataaa agcagtaatt taaactcagt tttttaatct
     6661 atttttgctg ctgagacaat acatttttgg aaaaatggtg gatgctgata acaactgata
     6721 accagcaaat aacataagca gtgccggtaa attctgtacc gataaagtgt gtgtgttcaa
     6781 ctaagagctg accgatgaat ggagagagca gatagagaag tccgatgagt atatgttctt
     6841 tgtgtttcat taaagcgcac cttctttcct tataggttga acgacttgca agtgttaaat
     6901 tgaatggtat gttatctagg taagcttact caaatgatag ttcccgcttc ctcctttcca
     6961 aagtaagcct tttcatactt atattttaca actttaaaaa taggaaatca aatcattttc
     7021 taagtaattt tgcttatatt tcacccaatc ttacaaaaat gtaagattaa agtctctttt
     7081 gtaaggttag gttatggcaa gcggtctttt tttgatgtaa actagactca taaagaacaa
     7141 aggagtaaca tcatgaaaca aatcttacat aagaaaacta gaaaaccaag tcacaaagaa
     7201 attgagcgcg ttcagctggg atgcactatg atgcaagcta catttcattt gatgggatat
     7261 taagaaagga gattctcatg acacttttag atgtaaaaca cgttcaaaaa atttataaaa
     7321 cacgttttca gggcaaccaa gtagaagccc tcaaggatat tcactttacc gtcgaaaagg
     7381 gtgactacgt tgccatcatg ggtgagtctg gttctggtaa atcaactctt ctcaatattc
     7441 tagctatgct ggataaacca agtcgtggtc aggtttactt gaatggaact gacaccgcaa
     7501 ccattaaaaa ttcacaggcc tctagcttcc gtcgtgaaaa gttgggcttt gtcttccaag
     7561 actttaactt gctagatact ctatctgtaa aggacaatat cttgcttccg cttgtcttgt
     7621 caagaagacc tattacagag atgatgaaga aattggtggt gacagctgag aatctgggaa
     7681 taaaccaatt acaagagaag tacccttacg agatttctgg tggccagaaa cagcgtgtag
     7741 cagtagcacg cgccatcatc actgaacctg aaattctcct tgcggacgaa ccgacaggag
     7801 ctcttgactc caagtcatct gcagccttac ttgatgtctt tgatgaaatc aatgagcgcg
     7861 gccaaaccat tctcatggta acccactcaa cagcagcagc tagcagagct aaacgtgtac
     7921 tctttatcaa agacggcatt ctttacaatc aaatctaccg tggagagaag acagaacgtc
     7981 agatgttcca agaaatctct gataccttga ctgtcatggc aagcgaggtg aattagtatg
     8041 tttcgattaa ccaataagtt agcggtatcg aacttgatta aaaaccgcaa actctactat
     8101 ccttttgcgc tggctgttct cttggcagtc actgtcacct atctctttta ctctctaacc
     8161 ttcaatccca agattgctga aattcgtgga ggaacaacca ttcaggctac acttggattt
     8221 ggtatgtttg tcgtcaccct tgcgtcagcc attatcgttc tctatgccaa tagttttgtc
     8281 atgaagaacc gttccaagga actaggaatt tatggcatgt tgggcttgga gaagcgtcat
     8341 cttatcagta tgacctttaa ggagttagtg gtatttggga ttctaactgt tggagcgggt
     8401 atcggtattg gagccttgtt tgacaagtta attttcgctt tcctgctcaa actaatgaaa
     8461 ttgaaggttg agctggttgc taccttccag acgaaagttg tcattacagt gcttgttgtc
     8521 ttcggtttga ttttcctagg cctcatgttc ctgaatgccc ttcgaatcgc ccgtatgaat
     8581 gccctccagc tctctcgtga gaaagctagt ggagagaaaa aaggtcgctt ccttcctctc
     8641 caaaccattc ttggttccat aagtttagga attggctatt atcttgccct tacggtaaaa
     8701 gatcctctta cagccttaac aaccttcttc atagctgttt tactggttat ctttgggact
     8761 tatctcttgt ttaatgcagg gattaccgtt ttcctccaaa tcttaaagaa aaataagaaa
     8821 tactattacc aaccaaataa tctcatatct gtttctaact tgattttccg tatgaagaaa
     8881 aatgcagttg gactagccac catcgctatc ttgtcaacaa tggttttggt aaccatgtca
     8941 gcagcgacaa gcattttcaa ttcctcagaa agctttaaaa aagttctaaa tcctcatgat
     9001 tttggggttt cagggcaaaa tgttgaaaaa gaagacttgg acaaactctt gagccagttt
     9061 gcaagtgaca aaggttatag tgtcaaagag aaagaagtac ttcgttacag taactttggt
     9121 attgcaaatc aagaaggaac taagttaact atttttgaaa aaggacaaaa ccgtgtccaa
     9181 ccaaaaacag ttttcatggt atttgaccaa aaagattatg aaaatatgac tggtcaaaaa
     9241 ctgtctctat caggaaatga ggtcggactc tttgcaaaga atgagggagt taaagaacag
     9301 aaagctctaa ctctaaatga tcatcaattt tctgtaaaag aagaatttac taaagatttt
     9361 attgtcaacc atgttccaaa tcagtttaat attttgactg ctgattacaa ttaccttgtt
     9421 gtacctgatt tacaagcctt tttggatcaa ttcccagatt cggctatcta taatcagttt
     9481 tacggtggta tgaatgtaaa tgccagtgaa gcagaacaac tcaaggtcgc tgaggagtat
     9541 gaaaaatact tacaaaagtt taatgctcaa ttaaacactg aaggtaacta tgtgtatggt
     9601 agcactctag cagatgctag tgctcagatg agtgccctct ttggtggtgt cttctttatc
     9661 ggtattttct tatccattat ctttatggtc ggaaccgttc tggtcatcta ctacaaacaa
     9721 atttctgaag gatatgaaga ccgtgagcgc tttattatct tgcagaaagt cggtttagat
     9781 caaaagcaaa tcaagcaaac catcaacaaa caggttttaa ctgtattctt ccttcctttg
     9841 ctctttgcct tcctacatct agcctttgcc taccatatgc ttagtctgat tttaaaagtg
     9901 attggtgtac tggatacgac tatgatgttg attgtgacct tgtctatctg cgctatcttc
     9961 ctcatcgcct atgtgctgat tttcatgatt acttcaagaa gttatcgcaa gattgtgcaa
    10021 atgtaaaaaa gatacctcga cttcaaaatc gaggtatttc ttgtattcta aatgctgaaa
    10081 agttgttcga gcaggaaggt aactcccatg gtcaagagac caatagcaag gttccgaatc
    10141 atagctgttt tggttggggc ttttccaagt ctagcacttg tgtaaccagt gagaagaagg
    10201 gccacaccga caataaggac ggtagcaggg atgcggtaat cacttggaaa aatggtcact
    10261 gacagcattg gaggcaaact tctaaggaaa aaggcaacga agctagaaat ggcagcgtgc
    10321 caaggattgg taaattcttc atactcaatc ccatattttt cctctaccag agccttgagt
    10381 ggatttttaa gaaagatctt attagtcaag agttgggcag aagttttgaa ttcttcattt
    10441 tggatataag cagcatagag ggattttttg gctagttccc tatcttggtc tagcaagagt
    10501 ttttctcgcg aaacggcagc ttcctcggta tcttttggag ttgaaacgga tacatattct
    10561 ccaccagcca ttgaaaaggc accagctaag atagccgcaa atcctgataa aaagataatc
    10621 cagatattgg tcgtggcact ggcaaccccg ataaccacac cagcaatgga aataattcca
    10681 tcgttagcat caagaacacc cgcacgcagg atatttaaac gacctgcaaa atttgaatca
    10741 atttcgtgat ttgtttctga cgctaaattt caagttcaag ttagccatcc a
//
LOCUS       DAVBCV010000066        10604 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBCV010000066 DAVBCV010000000
VERSION     DAVBCV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.066"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(7..1221)
                     /locus_tag="VQ516_001811"
     CDS             complement(7..1221)
                     /locus_tag="VQ516_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2512205.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1377..1670)
                     /locus_tag="VQ516_001812"
     CDS             complement(1377..1670)
                     /locus_tag="VQ516_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512206.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1832..2284
                     /locus_tag="VQ516_001813"
     CDS             1832..2284
                     /locus_tag="VQ516_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2512207.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2319..3707)
                     /locus_tag="VQ516_001814"
     CDS             complement(2319..3707)
                     /locus_tag="VQ516_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2512208.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFENLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3773..4735
                     /locus_tag="VQ516_001815"
     CDS             3773..4735
                     /locus_tag="VQ516_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2512209.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4732..4923)
                     /locus_tag="VQ516_001816"
     CDS             complement(4732..4923)
                     /locus_tag="VQ516_001816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512210.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDETDEVSSKHVFEVVDRTDEVS
                     NHTYGRATLTWFAEIFEEY"
     gene            complement(4987..5997)
                     /gene="ccpA"
                     /locus_tag="VQ516_001817"
     CDS             complement(4987..5997)
                     /gene="ccpA"
                     /locus_tag="VQ516_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2512211.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKVGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6422..7021)
                     /locus_tag="VQ516_001818"
     CDS             complement(6422..7021)
                     /locus_tag="VQ516_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2512212.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7022..8119)
                     /locus_tag="VQ516_001819"
     CDS             complement(7022..8119)
                     /locus_tag="VQ516_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2512213.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8119..8856)
                     /locus_tag="VQ516_001820"
     CDS             complement(8119..8856)
                     /locus_tag="VQ516_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2512214.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(8858..9742)
                     /locus_tag="VQ516_001821"
     CDS             complement(8858..9742)
                     /locus_tag="VQ516_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2512215.1"
                     /translation="MNMIKVQGLHKNIKSKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(9729..9923)
                     /locus_tag="VQ516_001822"
     CDS             complement(9729..9923)
                     /locus_tag="VQ516_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512216.1"
                     /translation="MKKAILFLFIGLSLVVWLVEMFTGWFDQSFLHQVIRGAWGLGFM
                     ISVVFPMGMEWLKGEYYEHD"
     gene            complement(9920..10126)
                     /locus_tag="VQ516_001823"
     CDS             complement(9920..10126)
                     /locus_tag="VQ516_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512217.1"
                     /translation="MEEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEGFYHFPWELFAS
                     FGIMSWLIREGLKLVRDVKKELEK"
BASE COUNT         3213 a         2411 c         1931 g         3049 t
ORIGIN      
        1 caagccctat ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa
       61 ctcatcaaca cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc
      121 tcgaccatga accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc
      181 atcatcgata cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg
      241 tttaacggca gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc
      301 gtagattcgt ccaccaggaa gaagcaattc atcgactgat tggtgacccc ccaaggaagt
      361 ttgtacgact tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag
      421 cccttcgata tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg
      481 gaaacctgct atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg
      541 tgccaagctc gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc
      601 atccgcaaag ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc
      661 cttaggataa agggctccag ttgggttatt tggattgata aggacaattg ccttggtatt
      721 ggaagtgatt tttgacttga tatcgtcaat atctgggtac cattccacag cttcatcaca
      781 gatatagtga acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc
      841 tggcattggc actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat
      901 caactcactg acaccatttc caaggtaaat atcatcaata tctacgtttg ggaatttctt
      961 cagctggcaa tactgcatga tggccttacg ggctgagaaa atccctttgg agtcagaata
     1021 gccttcacta tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa
     1081 gccaaattct gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat
     1141 ccgcatggct tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact
     1201 agacttgtta tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca
     1261 ctagaaagag aatgcgacaa gtttactgaa atgtgttact agtttcgtat agaagaaaaa
     1321 ccttgctaaa ggcgaggtta agaacgtaga gatagcaggc attttgcaaa gacacatcac
     1381 ataccaaaag ataaacctag aaaaagcaag cctagaaagg agtcataaag aagattaaac
     1441 atgaggaaaa gcccccatat catcaagtaa tcccaacgcc cacttcgttt tgcaactaga
     1501 aataagatta gatggtagag tacatgaaag actaaaaatc caataaaact tggataaaga
     1561 agcggtacca gactctctaa ttgacagatc atgatcactt ttaccaagac tgaaactagg
     1621 ataattccat aatacaggaa atttgatttt tgaaaagagt ttttcatcat tctcccttcc
     1681 ttcacttctt tccttctatc ctttctttta ttttatcata tatttatata gattttaagc
     1741 cctaatattc ttttgaaaaa tacttttttt gctttacttc tatagtgaaa aggtttacaa
     1801 ttatagtaag aaaatttcag gaggtttcat tatggcacaa cgttaccaaa atatcatggt
     1861 cgcaatcgat ggttctaagg aagcggactt ggcttttgtc aagggagttc attctgctct
     1921 acgaaacgac gctaaactca ccatcgcaca tgtcattgac acacgcgctc tccaaagcgt
     1981 atccaccttt gatgctgaag tttacgaaga actccaagtc gacgctgaaa gtctgatgaa
     2041 agagtacgaa aaacgtgcta aagatgctgg ggtagcagat gttcatatcg tcattgaaat
     2101 gggaaatcca aagaccctgc tagcacgtac tattccagat gccgaggaag tggacctcat
     2161 cctcgttggc gcaactggtc tcaacgcctt tgaacgcctc ttggtcggct cttcatctga
     2221 atacatactc cgccatgcta aggtcgattt gctggttgtg agagaacaag aaaaaacctt
     2281 ataatcacaa agaaaaggag cccctagctc ctttttgttt acgatttatt tctctcttta
     2341 tggcgttcgt aagccttgag ctggcgctgc agttcctttt taatagcagg ttctggagca
     2401 tatttttctt cccaattatc tggttttaag attttatggg tcactggatc aaaatgagcc
     2461 ttgccatctg gaaaaatttt ccccatattg gcctgatgga caatatcaaa aatacgttct
     2521 gggtccaccc ccatcaagac aaaactgccg taggtgaagt aaagcgtgtc aatcaaggca
     2581 tccacttgcc ctatcaaatc ttgctgagca ggtgtcttct tggctacttt acctgctgcc
     2641 ttatcaaggg cctgatgaag ttgcgataca gcttgaccaa aatcttcttc agaaggactg
     2701 gctgctcgaa caaactccac caattcttct attttaaaac cagccctatg ggttgcaccc
     2761 tctaaatccc aagctcgagg ttcttcttgg gttcgttcat ccatcatgtg gtggaaggtc
     2821 ttgaccttat tgaaatgata gtcacggctg acaaagactt tttctgaaga caaaacacca
     2881 aaatgttcca aggccttgtg gatcccatct tgttgattgc tagtggtaat gtgcttggca
     2941 gcttccttga cactgctact accatttccc atagcgaccg acataccgac gccagccagc
     3001 atttcaaggt cattatctga gtcaccaaaa gccatgactt ggttgaggtc aaagccatat
     3061 tctttcccaa ctcggcgaat gccttctaat ttagaatttc cctgattgat gacatccgat
     3121 gcaaaaggat tgctacgtgt caatttcaag ttttcaaaat cagctgccgc cttctcagat
     3181 tcttctggtg tcatcagcat caaaacttgg tagataggct gattcatcag gtgaagcagg
     3241 tcctcttcct tttggggaac aaccttgctg accatgcgat taaaagactg actcaccgtc
     3301 cgagttaaaa cagagggaac gaagcgacta attcgttggg aaaaagaacc cagaccaaag
     3361 gacatgattt tagaacccaa catggcatcc ttggtcccta gggcaatctc cgtgccctct
     3421 tttttagcat agctaattag atggcgcaaa tgtaacttgg aaatagggct cgtgaacaag
     3481 actctgtctt tactaaagat atactggcca ttataggtta ccgcaaaatc cagatccaaa
     3541 tcgtccatca attccttaac aaaaaaaggt cctcgccctg tcgctacgcc aactagtacc
     3601 ccttgttctt tgacaatctt aatcgcgtcc ttagtggatt tcaaaacact cttgcgattg
     3661 ttgaccaagg ttccatcgat atcaaaaaaa acagctttga cttccatcct attccaatct
     3721 ccccttttgt gatacaatga ttataccaca tttcagaaag agtgagtaaa tcatgcctaa
     3781 gaaaatcctt gttttacata cgggtggaac tatttccatg caggccgatg cttctggcgc
     3841 tgttgtgacg agttcagata atcccatgaa ccatgtgtcc aacccacttg aaggaatcca
     3901 agtccacgcc ttggactttt ttaaccttcc aagtccccat atcaaaccca aacatatgct
     3961 ggtcctctac cagaaaatta aagaggaagc agataactac gatggagtgg taatcacaca
     4021 cggaaccgat actttagagg aaacagccta tttccttgat accatggaag ttccccatat
     4081 gcctatcgtt ctaacaggag ccatgcgtag ctccaatgag ctcggtagtg atggtgttta
     4141 taattaccta agtgctttac gagtggccag cgatgacagg gctgctgaca aaggagtttt
     4201 ggtcgttatg aacgatgaaa tccacgctgc caagtatgtc accaaaacac atacgactaa
     4261 tgtcagcacc ttccagactc caacacatgg ccccctcggt ctcatcatga aacaggaaat
     4321 cctctacttc aaaacagctg aacctcgtgt tcgctttgac cttgatcaca tacaaggttt
     4381 agtccctatc atctcggctt atgctggtat gacagatgag ctgattgata tgctggattt
     4441 agaacacttg gacggtttga ttatccaagc cttcggagct ggtaatattc ccaaagaaac
     4501 ggctcaaaaa ttagaaagcc ttctgcaaaa aggaatccca gtcgctctgg tatcacgatg
     4561 ctttaacggt attgccgagc ctgtttatgc ctaccagggt gggggcgtac agttgcaaaa
     4621 agcaggcgtt ttctttgtta aagaactcaa cgcccaaaaa gctcgcttga aactcctcat
     4681 cgccctcaat gccggactaa caggacaggc tttgaaagac tatatggaag gctaatactc
     4741 ttcgaaaatc tctgcaaacc acgtcagagt cgccctaccg tatgtatggt tactgacttc
     4801 gtcagttcta tctacaacct caaaaacatg ttttgagctg acttcgtcag tttcatctac
     4861 aacctcaaaa cagtgtgttg cgcagccttt aaccagccgc ctagtccgct ctatggtatt
     4921 cattaagtca acatctcttg tttaagagca caaaatcagg aaatcttctc gattccctga
     4981 ttttttctat ttacgttttc gtgttgagct acgttctgtc aaaccatgag gtaagagaac
     5041 ttcacgttct tccaactctt ccttatgcat aatcttggtc aacatacgca tactaatggc
     5101 accaaggtca taaagaggtt gggcaatcgt tgtcaagttt ggacgggtaa agcgtgagat
     5161 ttgtgaatca tcactagtaa taatttcaaa atcttctggc acagaaacac ccttatcagc
     5221 caaaccgttc aagactcctg ctgccaactc atcacctgtc acaactgctg cagttgcatt
     5281 tgatgaaatc aaacgctctg ctaaggcgta accatcatca tagctatatt tagattcaaa
     5341 taccaaaccc tcactataag tgattcctac ttttttcaag gtttccttgt agccaactaa
     5401 acgaacctta ccattgatgt catccactag cggaccgcta acgaaagcaa tacgctcatt
     5461 ttctttagca aggtagctca ctgcatcaat tgttgcttgc ttatagtcaa tattgacact
     5521 tggcaactgg tgctcaacat cgacagttcc tgcgagaaca atcggagtac gtgaacgcga
     5581 aaattctgag cgaattttat ctgtcaagtg ataacccata tagataatgc catctacctg
     5641 ctttgaaaag agggtattga caacagaaac ttctttctcg ttatcttcat cgctattagc
     5701 taggacaata ttgtacttgt acatttctgc aatatcatca atccccttag ccaaactcga
     5761 aaaataacca ttggtaatat ttggaatcac gacaccgaca gtggttgtct ttttacttgc
     5821 aagaccacgc gcaactgcat ttggacgata atccaaacga tcaattacct ctagcacttt
     5881 tttacgggta ttctctttta catttttatt gccattgacc acacggctga ccgtcgccat
     5941 ggaaacacct gcttcacgag cgacatcata aatggttact gtatcatctg cattcattcc
     6001 ttttcctgtc ctttctatct cacacattct tttacaagta gaggtactga ttgaagctct
     6061 atatctactt acaaaagtga agatgtgaaa atttcgtttt catatttcta cttattccat
     6121 tctatcacta attgtaaaca ctttcaagtg ttttttgaag attgattgaa aaaatttcat
     6181 agaaaaccta ggtttagctc cttgctacca ccttagacta aataaaaagg aggaaactaa
     6241 gcccttctaa agttatagta aaatgaaata agaacaggat aaatcgatca ggacagtcaa
     6301 atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca atctactata
     6361 ggtattgttc cattcactac cgtcaatttt agcacatagt cttcatgaaa atattatatc
     6421 atcataacca accagattct tttgcgatat tagctgcctc tgttcgatta ccagcatcca
     6481 gttttgaaag aatattggtg acatagtttc gaatagttcc gtttgagagg taaagctgat
     6541 ctgcgatttc ttggttggat aagccccggg cgattccctt taagacagca atctcttgtt
     6601 ctgtcaacgg attgggacgg gtcatcacca cttccatcaa ttcaggcgaa tactccttgc
     6661 gtccttcgag gacggtgtgc aaggtttgca tgaggtctgc aatgcttcgt tcctttaata
     6721 cataagcatc cactccagcc ttgaccgcac gttcaaaata cccagcacgc ttgaaggtcg
     6781 tcaccacaac cacctttgtt tcaagccttt ctgatcgtat ccactccaag acttcaagac
     6841 ctgtcttaac aggcatttct acgtcaagga tggcgatatc tacagactcc ttttctaata
     6901 gttggattgc ttcttgccca ttcttggctt gaaggacaga ctccacatct ggttgaagcg
     6961 tgagcaattg acacatagca tctcgcaaca tactttgatc ttctgcgact aatactttca
     7021 tctactttct ctccttataa ggtagtcgaa cttgcacttc cgttggatgt ttctgactga
     7081 ttacacttac ttctcctgaa aatggaagga cacgttctcg aactgtatgg agctcatttt
     7141 ctgttacagt ttcaaaacct ttcccgtcat ctctgaccat caggagcaat tcctgatctg
     7201 tgcgttctaa ttttaagtag gctttagacg ctttagcatg tttgatgatg ttggtcacta
     7261 attcaagcaa aatcatggaa gccgttgact ctaattcctg agttaagcta gctgtatcta
     7321 gttgattagc tatttccacc tcaattccag caatttctaa catctttttc acagtctcta
     7381 gttcggatgt caaagttcta gacttaagat tttccacaat ggttcgcact tcacgcattg
     7441 attctttgct aatctgctgt atttctctta attccttttc cacttgtgga taagcctgta
     7501 tttgaaataa ctgcaaggct agatcagttt taacactaag catagcaaag gtatgcccca
     7561 gactatcatg caaatcttga ccgatacgat tgcgctcatt ttctgctagc aatagattta
     7621 tctgggtatt ttgcttagtc tgagcttctt tcaaatcctc gacaaggcga attcgaaccg
     7681 agcccaaagt catcaaatca acaaaagtaa gaattccaag cagatagact agaaactcaa
     7741 cttcgattct ctgaaaaatc aaaagtcgcc ccacaacaag gacttgagca agaagaaaag
     7801 tccagacatg taaagacttt aaactacgta cgctgaaatg ataacttaag agattggaaa
     7861 ggaaaaagaa aaaccagata taattcacag caacaaaggc ggtattccca gctacataga
     7921 tcagcatgat cccccagaaa agccaagata ggcgctggct cttagttgtt aaaacaccca
     7981 aatatgccac tacaaataga atatcaatca ataaatgcca ggcagaaagc cacccagtca
     8041 ctacaggtag gatggggaaa atcataaaaa ttaaactggc ccaaaacata tagtgtatgc
     8101 ttttcagtct ttcaagcatt aagcattctc cttatgacct tgaaggtaaa tggtcaaacc
     8161 aaacaaaact gctgaaaaaa caagtagata aactgtggct gatagattga tgctaccctc
     8221 gtttaagaag gtcttgagca actccatcaa ctgataagtt ggtaggcact tcccgattgc
     8281 ttgcatccag tctggaaata aagagatggg catccagagt ccacctaaaa cagccaagcc
     8341 tagatagaga agattgccca cgacagacat cagctgacta gaaggcaaga gtgtcaaggt
     8401 caagccaagc gctacaaaag ctatacttcc tactatcagc aagaacgcag ccccaatcca
     8461 gcttcctaaa gacatatcca cacctctgac caagtgccca actgagaaaa caaccaggat
     8521 tgagaccaaa taatcaacca tcatacttgt tatctttgat agataatatt ctaccatatt
     8581 tacagggcta tgacgcaatg ttttctgcca gttgttcatc ttatcggtat gtaagacagc
     8641 tgggaatgaa aagatagcgg tcgacatcat ggaaaaggca gtcatggaga tgaggtagtc
     8701 gcgcataaaa ttagccggtc cacctggtgt gtcctggtac atgcctgaaa agaatagata
     8761 gaaggctgtc ggcatcccta cagataataa ataatagact gattgccgtt tggtcaatag
     8821 aaattctatc ttgtttagtg caatccatcg tttcatctta gtcatctccc ttctgtgttt
     8881 cttcaaagat tgtatccaac aaactacgat tattgacttc gatttcttgt atcctacatc
     8941 ctgcttgaac taacagttcc cagaaagcat ccgcttcgca tgtgactact tgtaaggcat
     9001 cttgtttttg tacccagttt tcaaccaaga ctgacttctc aacgacttcc ttgtaagcta
     9061 gcggaaggat aaaatgcttt tcaatctcct cactacgcat agctagaggc gtcgtgtcac
     9121 gaatcaactc tcccttattt aaaaccaaaa tccggtcagc tgtatgctct acctcttcaa
     9181 tataatgaga cgaatagaga atggtgactc cttgcgcttt taggtcccga acaatttccc
     9241 aaaaacgttg acgagttgaa gtatccatgg cagcagttgg ctcatctaaa aagacaagct
     9301 ttggtcgccc aattaaggtc aagataaaag ataaaagacg cttttgccca cctgacaatt
     9361 tttctgcgaa ttgctctttc tgttgctggt caaactgtaa tagttgatcg atttcctggt
     9421 tgttcaaggg atttggatag atacgttgaa agaaagcaat caactcttta acctttagtt
     9481 tttgaacaat tacattttct tgagggagat aggatctcgt atagtctaac ttagagctcg
     9541 ttaccggcaa atcttggata tatacttgcc cactggtgac caatttatct ccaagcaaac
     9601 agtccaagag tgtcgtctta ccagctccat ttggcccaat caaggcgacg cattcacctt
     9661 cagctacctc aaaggaaata tccttcaaaa tagccttact tttgatgttt ttatgtaagc
     9721 cttgtacctt aatcatgttc ataatattct cctttcaacc actccattcc catagggaaa
     9781 acgacggaaa tcataaatcc taatccccag gcgccgcgaa taacttggtg aagaaaggat
     9841 tgatcaaacc aacctgtaaa catttctacc aaccatacca ctagtgatag tccaataaaa
     9901 agaaagagaa tcgctttttt catttttcaa gctccttttt cacatctctt actaatttca
     9961 agccttctcg tatcaaccaa gacatgattc caaagcttgc aaacaactcc caaggaaaat
    10021 gatagaatcc ttcgtccaat cctgagaaca tgagataagt catgactcct gctactacta
    10081 aactcactgc gataatcatt ttatttctca tctcttcttc ctccatttca tactacaatt
    10141 atagtctttt gaaatcagag gagatagaag cttctgtcac taaaaaatat gacaaatgtc
    10201 ataaaaaatt ctgttcaaaa caagcaagat acactataca ataaaacaca attagataaa
    10261 tctaaggcaa cttcctcaaa agagatatca aaccccattc acactataat gtaaactaat
    10321 acttatttaa agtcaaaaga gcggaaattt ttatcgtaca aacatatata ttataggtaa
    10381 gacaaaaaac tcttcaatta ctttcttaaa aatttatagg agttataaaa tctattaaat
    10441 ttcaatgtgg atacctaaac tacagtattt attgaaaaga ctggagacaa aaagtatacg
    10501 ctgccaaaat gaattgctga aaatcaaaaa agagagaagc aaactgattc tctcttaatg
    10561 tatataatat ctagttataa aaatacacac tcacatatct ctgt
//
LOCUS       DAVBCV010000067        10222 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBCV010000067 DAVBCV010000000
VERSION     DAVBCV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.067"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(365..1138)
                     /locus_tag="VQ516_001824"
     CDS             complement(365..1138)
                     /locus_tag="VQ516_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2512218.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            1314..2717
                     /locus_tag="VQ516_001825"
     CDS             1314..2717
                     /locus_tag="VQ516_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2512219.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            2832..3470
                     /locus_tag="VQ516_001826"
     CDS             2832..3470
                     /locus_tag="VQ516_001826"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET2512220.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            3482..3922
                     /locus_tag="VQ516_001827"
     CDS             3482..3922
                     /locus_tag="VQ516_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET2512221.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            3915..4346
                     /locus_tag="VQ516_001828"
     CDS             3915..4346
                     /locus_tag="VQ516_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2512222.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEENLCL"
     gene            4337..4807
                     /locus_tag="VQ516_001829"
     CDS             4337..4807
                     /locus_tag="VQ516_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2512223.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            4832..5605
                     /locus_tag="VQ516_001830"
     CDS             4832..5605
                     /locus_tag="VQ516_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2512224.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            5602..6402
                     /locus_tag="VQ516_001831"
     CDS             5602..6402
                     /locus_tag="VQ516_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2512225.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            6461..8755
                     /locus_tag="VQ516_001832"
     CDS             6461..8755
                     /locus_tag="VQ516_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2512226.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPVNLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            8765..>10222
                     /locus_tag="VQ516_001833"
     CDS             8765..>10222
                     /locus_tag="VQ516_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512227.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLH"
BASE COUNT         3406 a         1387 c         2142 g         3287 t
ORIGIN      
        1 tatatttaga tcatcaaggt cacttaattc ttgaaatata gggtctaggc gacaaagtct
       61 ttaaaatcag aaaaacgcat aatatcaggt gtgcaaaaac ttgatactat gcgttttatt
      121 gtgggaaggt ttactccatt ttctcctgaa attgagtttt tgtccagcct ctgtttttag
      181 aattgctaag aaaataatgt catgtggtga atatttgtaa atcagtcagc agacagaacg
      241 aatactcttc gaaaatctct tcacatcatg tcagcttcgt cttgccgtgt ccatggggga
      301 cttgacttga tcagttttca aatctgtact ttgagcaagc tgagactagc ttcctatttg
      361 attttcattg aatatcagaa acccattctc catcaaataa ttcgactgcg tctaataatt
      421 tttggtctgg caaggtgtca gaaataaagg ttgtgtattt ggagagggga ttaattttaa
      481 aaaatccagt cttgtaaaat ttagaactat caatcagtaa gatggtttca tgggctttgt
      541 caataatatt cttttttgaa atagcttggc tgagagaagc ttcataaaca tattggtcat
      601 caatacctct tgctgaacaa aatgctaaat cgatattaaa atgatctaat aaagaatttt
      661 ccttatcata gttgaccacg gaacaggatt gatgtttgac ctcgccagat gtgataaaga
      721 ttttggagct atctttaaca gtttcagata gggtttgtgc agtatgtaaa ccatttgtaa
      781 aaataatcaa attatcaagt tcagaaagat agggacaaag ttcgtagaca gtagtactag
      841 aatctagata gatacacata ccagaccgaa taaagtcttt agcgagacta gcgattagtc
      901 ttttttgcct agtactttct ccttcccgta tttgatgaga aagttcaatt gtgttcatag
      961 aggacagggt cacgtatccg tgctttcttt tgataagacc ttgattttct aagaaaatta
     1021 aatcacgacg taaggtactt gtgctggaga aagtgatttc tgccagctct tttacggcaa
     1081 ttcttttttt ctttttaata atttcaatca attcaagtac acgttcatct tttatcataa
     1141 gctcctccta atttatcatt tcaactatat tatagcacaa attggaggaa tttgaattat
     1201 ttttatgaat attgggttaa catttgaaca ttattcaagt aagcgttcac atattgaaaa
     1261 aataaaacgt ggggattata ataaagttaa tcaaggacga agagagaaga aaaatggaag
     1321 cggttttagc aatagattta ggtgcgactt ctggaagagc aatcgttggt tacctttctg
     1381 aaaataaact agtaatggaa gaaataaatc gcttttctaa tctacctatt agagtaaaag
     1441 ggcatttatc ttgggatatt gactttctac tagctaaaat tcttgaaagt atccgcttgg
     1501 ctaatactag ttacaagatt ttatctatcg gtattgacac atggggagtt gattttggac
     1561 tgattgataa tgaaggtaag ctgttatcac aacctgttca ttatcgtgat gaaagaacaa
     1621 agggagtgtt aaaggaaata tctgaaatga ctgaattaga aaaactgtat tcagagacag
     1681 gaaatcagat tatggagata aataccttgt ttcaactctt taaggcacgt caagaatctc
     1741 ctgactcttt ctataagacc aataagattc ttttaatgcc agatttgttt aattatctct
     1801 tgacaggtaa gtttgctaca gaaaaaagca ttgcttcaac aactcaatta tttgatccta
     1861 ggagtcaaaa ttggaatcag aatatcttaa aactatttga attggattca tctttacttc
     1921 ctgaaattgt ttcagaggga aatgttcttg gaaggataaa aaaggagtat ggtttaggcg
     1981 atattcctgt tgtgaatgtt tgtagtcatg atacagcaag tgcgattgtc tcagtaccta
     2041 agacagaagg tagtttattt atttcatcag gtacttggtc tttggttgga gtggaactta
     2101 cttcaccgat tcttactacc gaatccttca gttatggatt tacaaatgaa gtcggtaaag
     2161 atggagtgat tacatttctg aagaattgta cagggttgtg gatcatagag gaactaagac
     2221 gttcatttga acgaagaggg aaagcctatt cttttgatga tattaggaca atggtggaga
     2281 aagaaaaaga aaatcttcct ctgattgata ctgaatcaac tgaatttgca acagaatctg
     2341 atatgcacaa gactttgaca gaatatctag cttatcatca tgaaactaga gagtggacag
     2401 atggacaact atttaagatt gtttatgaaa gcctagctga aacgtatagg aaagcgatag
     2461 agttattaga agaactaact cataaggttt ataagaggat atatgtgatt ggaggaggtg
     2521 ctagagccag ttattttaac caaatgattg ctgatagaac tggtaaagag gttcttacag
     2581 gtttgactga ggctacagct gtggggaata ttgttgtgca gctcatagct atgggacaat
     2641 taaaagggat ggaagaggct caccatgtta ttgaggagtt tctacaatta gagagttatt
     2701 actcccaaaa gaattaaaaa gattgagagt ttgtaaattt gcctccctcc cccttcttag
     2761 cttttgtgca ggaagggggg ataattggtg aattgaaaaa tatttagtgt tttgatatga
     2821 ggaggacaag gatgtcagat gtaaaacaag aattaattaa atatggtaag aagctagtag
     2881 aaacagattt gacgaaagga acaggtggga atctcagcgt tttcgatcgt gaaaaacaat
     2941 tgatggcaat taccccgtcg ggtattgatt tctttgaaat caaagaatcc gatattgtag
     3001 tgatggatat taatggaaat gttgtagagg gagaacgctt gccatctagc gaatggtata
     3061 tgcatttgat tcaatatcaa actcgtgatg atatcgatgc aattatccat gctcatacaa
     3121 cttatgcaac agtattagct tgtctcagag aaccacttcc agcgagtcat tatatgattg
     3181 cagtggcagg gaaagatgtt cgggtagctg agtatgcaac atatggcacg aaagaattgg
     3241 ctgtgaatgc agctaaagca atggaaggtc gtagagcagt tttactagcg aatcatggaa
     3301 ttttagcagg tgcacaaaat ttattgaatg catttaatat tgttgaagaa gttgaatatt
     3361 gtgcaaaaat ttattgttta gctaagaatt ttggagagcc agtagttctt cctgatgagg
     3421 agatggaatt gatggcagaa aaatttaaaa catacggtca gagaaaatag ggaggatatt
     3481 aatgttaaaa catataccga aaaatatttc tccagattta ttgaagactt taatggaaat
     3541 gggacatgga gatgaaatag tattagctga cgcgaattat ccttctgcct catgtgcaaa
     3601 taagctaatt cgttgtgatg gcgtaaatat tccagaatta ttagattcca ttctgtattt
     3661 aatgccatta gatagttacg tcgatagttc aattcagttt atgaacgttg tttcgggtga
     3721 tgatattcct aagatatggg gtacctatag acagatgatt gaaggtcatg gtacagatct
     3781 taaaacgatt acttatctta gaagagaaga cttttatgaa cgtagtaaga aagcttatgc
     3841 tattgttgct acaggagaaa cttcacttta tgctaatatt atccttaaga aaggagtagt
     3901 tgttgaaaga gaaaatgttt aatagaggaa ttttagttgc cagtcatggt gattttgcta
     3961 gcggagctct catgaccgca gaaatgtttg ttggtgagac aacaaatgat agagttagga
     4021 cattaggttt gatgcctgga gagaatattg tagagtttga gcattatttt aaaaatcaag
     4081 tggatgaact gttagactca aatcaagagg ttatcgtttt gactgacttg attggaggaa
     4141 gtcctaataa tgtggctttg tcacggtttt taaatttgga ttcagttgat attgtaacag
     4201 ggtttaatat tcctctccta gtggaattaa tatcaaatta tgattcaaaa atcaatttag
     4261 aagaaattgt tcacaatgct caaaatagtt tgtttaatgt taaacaacaa cttaacgtag
     4321 aggaggaaga aaatttatgt ctatagagtt tgttcgtatt gatgaccgtc tggtacatgg
     4381 tcaagttgtc actacgtggc taaaaaagta tgatattgag caagttatca ttgttaatga
     4441 tcgcatctca gaagataaaa cacgacaatc tattttaaag atttctgcac cagtaggttt
     4501 aaaaattgtt ttctttagtg taaaacggtt tgtggaagtt ttaaactctg tgccaataaa
     4561 aaagagaaca atgctgatat atacaaatcc aaaagatgtg tatgattcta ttgaaggaaa
     4621 tttaaaattg gagtacctca atgtaggaca gatgagtaaa acggaggaaa atgaaaaggt
     4681 aacgggaggt gtagctctag gtgaagaaga caaatattat tttaagaaaa tagttgataa
     4741 gggaacgaga gttgaaattc aaatggttcc taatgataaa gttacaatgt tggaaaaatt
     4801 tttataaaaa taatttaagg aggtacagta tatgctattc acacaagcat tactggtgac
     4861 attagttggg attattgcca ctattgacta taatggaccg ttatttatga ttcaccgtcc
     4921 gttagttaca agtgcaatgg ttggcttagt cttaggagat ttcacccaag gtgttcttat
     4981 tggttcagct cttgaattaa cttggctcgg tgtaacaggt attggaggtt acactccacc
     5041 agatactatt tcaggtgcga ttattggtac tgcatttggt attttatctg gtcaaggaga
     5101 aactgctggt atcgctatag cagttccaat tgcagttgct acccaacagt tggatgttct
     5161 tgcaaaaact ttagatgttt attttgtgaa aaaagctgat aatgatgcta aaaacggaga
     5221 ttattcaaag atcggttttt atcattattc aagtttggtt ttaatcacgt tatttaaaat
     5281 tgtaccaatt ttcctagcta ttatgcttgg aggggaatat gtggcagact tgtttgctaa
     5341 ggttccacca atcgttatgc agggacttaa ctctgcaggt gctttactac cttcaattgg
     5401 ttttggtatg cttttaaata tgatgctcaa gaaaaatatg tgggtattct tgttgattgg
     5461 attcatttgt tctgtgtatg gaggaatgtc aaccattggg atctcactag ttggtattgc
     5521 ggtagcatac ttctacgata tgattggaag taaaccacaa gaaacaactt caagtagtga
     5581 tgttgaggag gatcttgatc tatgatgaat aataaagtaa ctaaagttga acttaaaaaa
     5641 gttttcaaac gaagttttat gtatggttct tcatggaact atgagagaat gcagaaccta
     5701 ggttttctat atacaattct tccagtattg aaaaaactat acccagacaa agattcagct
     5761 tctcctgcaa tgaaacgtca ccttgagttt ttcaatactc atcaaacagc ggcaccattt
     5821 attcttggag ttacttccgc tatggaagaa caagaaggaa atgaaggtgc agcttcaatt
     5881 actggtatta aagttggctt gatggggcca ctggctggtc taggagatag tttgttctgg
     5941 ctgacactag ttcctatctg ttttagtatt ggtgcgtctt attctaaaga cggcggtgct
     6001 ttaggtatct ttatcgcctt aatattgttt aatattatta atattcctgt taaatatttc
     6061 ggtttgaaat atgggtatac taagggttct agtcttatcc aagaaaataa tacaaaagga
     6121 acattgaatc gcgttacgag tatggcgaca gcattagggc tagtactagt gggtggtttg
     6181 attccatcaa tggttggtat taattttgga ttagaattta agcaggggga acttgttatt
     6241 tctgttcaag aaatgattac aaaattaatt ccaggattta tccctatggc tttgacttta
     6301 ttaatgtgta aattaattag aaaaggaaag aatccggttg tactaatctt tagtgttatg
     6361 gctattggag ttatcctagt tgttttagga attttgaagt agtagaaagt gtggaggtgg
     6421 tatttgggat atcacctcca ttttggaaga gaggtaaaga gtgaaattat ggtataagaa
     6481 agctgcctca aattggaatg aagccttgcc gattgggaac ggtcatttag gtggtatgat
     6541 ttatggttca gctacaaaag aatgtattca actaaacgat gagactattt ggtatagagg
     6601 aaagtcagat agaaataatc cagactcact attgcatctt aaaaaaattc gggaatatct
     6661 tttagatgga gaaattcaga aagccgaaga attgataaag ttaacaatgt ttgctacccc
     6721 aagagatcaa agccactatg aattacttgg ggaactttac attgagcata tagatattca
     6781 gtcttgtgct ctttcattgt atgaaagaga gctagattta gatacagcta tttctaatgt
     6841 tgtgtttgag cctaatagtt gtaatttaca aataaaaaga gaatatttta cgagttttaa
     6901 taagaatatt ttatgttgcc gtatagtgtc atcagttcaa aacacattaa atttaaacat
     6961 taatttgggt agaaataaac ggtttaatga cgaagtatct aaactggatt caagtacaat
     7021 tttaatgtcg gcctctgctg gaggtagaaa aggtgttcag tttaaagtag tatgtcattc
     7081 taaagttacg gatggtgaag taagtgtatt gggagagaca atagttattc ggaatgctac
     7141 agaggtattt ctttatctca aatcaatgac ggattattgg ggaaatatag atatttcttc
     7201 tcttcaggga gaatttagta gtattgatta ctttacagaa aaagatgaac atgtaaaaaa
     7261 atatcaggag caatttaata gagttgattt taaactagac tatagtaaag attgtcttag
     7321 cattccaacg aatctacttc ttgaaaacac taaaaagtat agtaactact tgactaactt
     7381 gttatttcat tatggaagat atctgttaat atcgtctagt caaccgaatg gtttacctgt
     7441 caatcttcaa ggaatatggt gtgatgaatt aaatccaatt tggggttcta aatatacgat
     7501 taatattaat actcaaatga attattggat ggtaggtcca tgtgatttac cagaagtaga
     7561 atatccatta tttgatatgc tcgaaagaat gagagaaccg ggaagactaa ccgctaagaa
     7621 aatgtatgga gctagaggtt ttacagcaca tcataatacg gatggttttg gcgatacggc
     7681 tccccaatct catgccatgg gggctgcaat ttgggtatta actattccat ggttatgtac
     7741 tcatatttgg gaacactatt tatatttcca agatgagcgt attcttacgg aacattttga
     7801 aatgataaaa gaagcatttc ttttctttga agattattta tttgaggtgg atggctactt
     7861 gatgacaggt ccaagtgtct caccggaaaa taaatatcgc ttaaaaaatg gtattgaagg
     7921 aaatgcttgt ctatcatcta caattgataa tcaaattcta agatattttt gtgattcatg
     7981 cattggcatt gcaaaacaat taggagacaa ttcggatttt attagtcgtg tgaaggagtt
     8041 aaaaaagaaa ctacctaaaa caaaaatagg tagtaatggg caaatccaag aatggttaga
     8101 agattatgaa gaagtagagc ctgggcatag acacatttca cctctatttg ggctttatcc
     8161 ttataatgag attgatattc ataaaactcc ggaattagca gaagcagcta aaatcactat
     8221 caataggaga ttatcaaacg ctaacttttt atcttcacag gagagggagc aagcgattaa
     8281 taattggtta gtaagtggtt tgcatgctag tacacaaaca ggttggagtg ctgcatggct
     8341 gattcatttt tttgcgagac tatatcaagg tgaacctgct tataaccaga ttaatggttt
     8401 gttaaataat gcgactcttg gcaatttatt tcttgaccat ccaccatttc aaattgatgg
     8461 taatttaggt ttggtgagtg gaatttgtga attattagta cagagccatc ataattggtt
     8521 atcactaatt ccagctttac cttctgcttg gtcagaagga gaagtgaaag gtttcagagt
     8581 aagaggagga tataaggtat cgtttgcttg gaaaaatggg gatataacat tcctaaaatt
     8641 ggaaggagga aacaaagatc aaaaagtaag agtaagaata tatggcaaaa atactgatgt
     8701 acaaaatatt gaattggtat ttaattcaga aaaaattatt gagttaaatt tttaggtata
     8761 agtcatgaat aaagaaaaaa taaaaagaaa attaatcaca atactgtttg tatgtattgg
     8821 gatgttatgt tttggattgt tagcaggagt taaggctgat aatcgtgttc aaatgagaac
     8881 gacgattaat aatgaatcgc cattgttgct ttctccgttg tatggcaatg ataatggtaa
     8941 cggattatgg tgggggaaca cattgaaggg agcatgggaa gctattcctg aagatgtaaa
     9001 accatatgca gcgattgaac ttcatcctgc aaaagtctgt aaaccaacaa gttgtattcc
     9061 acgagatacg aaagaattga gagaatggta tgtcaagatg ttggaggaag ctcaaagtct
     9121 aaacattcca gttttcttgg ttattatgtc ggctggagag cgtaatacag ttcctccaga
     9181 gtggttagat gaacaattcc aaaagtatag tgtgttaaaa ggtgttttaa atattgagaa
     9241 ttattggatt tacaataacc agttagctcc gcatagtgct aaatatttgg aagtttgtgc
     9301 caaatatgga gcgcatttta tctggcatga tcatgaaaaa tggttctggg aaactattat
     9361 gaatgatccg acattctttg aagcgagtca aaaatatcat aaaaatttgg tgttggcaac
     9421 taaaaatacg ccaataagag atgatgcggg tacagattct atcgttagtg gattttggtt
     9481 gagtggctta tgtgataact ggggctcatc aacagataca tggaaatggt gggaaaaaca
     9541 ttatacaaac acatttgaaa ctggaagagc tagggatatg agatcctatg catcggaacc
     9601 agaatcaatg attgctatgg aaatgatgaa tgtatatact gggggaggca cagtttataa
     9661 ttttgaatgt gccgcgtata catttatgac aaatgatgta ccaactccag catttactaa
     9721 aggtattatt cctttcttta gacatgctat acaaaatcca gctccaagta aggaagaagt
     9781 tgtaaataga acaaaagctg tattttggaa tggagaaggt aggattagtt cattaaacgg
     9841 attttatcaa ggactttatt cgaatgatga aacaatgcct ttatataata atgggagata
     9901 tcatattctt cctgtaatac atgagaaaat tgataaggaa aagatttcat ctatattccc
     9961 taatgcaaaa attttgacta aaaatagtga ggaattgtct agtaaagtca actatttaaa
    10021 ctcgctttat ccaaaacttt atgaaggaga tgggtatgct cagcgtgtag gtaattcctg
    10081 gtatatttat aatagtaatg ctaatatcaa taaaaatcag caagtaatgt tgcctatgta
    10141 tactaataat acaaagtcgt tatcgttaga tttgacgcca catacttacg ctgttgttaa
    10201 agaaaatcca aataatttac at
//
LOCUS       DAVBCV010000068        10133 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBCV010000068 DAVBCV010000000
VERSION     DAVBCV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.068"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            398..1091
                     /locus_tag="VQ516_001834"
                     /pseudo
     CDS             398..1091
                     /locus_tag="VQ516_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1147..1419
                     /locus_tag="VQ516_001835"
     CDS             1147..1419
                     /locus_tag="VQ516_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HET2512228.1"
                     /translation="MFLLFSALVGMLSQLKEVPESVFLQETVEENHLVNVYSVLEVIS
                     TLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK"
     gene            1733..1864
                     /locus_tag="VQ516_001836"
     CDS             1733..1864
                     /locus_tag="VQ516_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2512229.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            1885..3435
                     /gene="dltA"
                     /locus_tag="VQ516_001837"
     CDS             1885..3435
                     /gene="dltA"
                     /locus_tag="VQ516_001837"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2512230.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFVQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRKRFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3432..4676
                     /gene="dltB"
                     /locus_tag="VQ516_001838"
     CDS             3432..4676
                     /gene="dltB"
                     /locus_tag="VQ516_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2512231.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4690..4929
                     /gene="dltC"
                     /locus_tag="VQ516_001839"
     CDS             4690..4929
                     /gene="dltC"
                     /locus_tag="VQ516_001839"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2512232.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            4922..6190
                     /gene="dltD"
                     /locus_tag="VQ516_001840"
     CDS             4922..6190
                     /gene="dltD"
                     /locus_tag="VQ516_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2512233.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6345..6785
                     /gene="adcR"
                     /locus_tag="VQ516_001841"
     CDS             6345..6785
                     /gene="adcR"
                     /locus_tag="VQ516_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2512234.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6785..7489
                     /locus_tag="VQ516_001842"
     CDS             6785..7489
                     /locus_tag="VQ516_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2512235.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNTHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7482..8288
                     /locus_tag="VQ516_001843"
     CDS             7482..8288
                     /locus_tag="VQ516_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2512236.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGRSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8298..9803
                     /gene="adcA"
                     /locus_tag="VQ516_001844"
     CDS             8298..9803
                     /gene="adcA"
                     /locus_tag="VQ516_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2512237.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKVTFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT         2903 a         1910 c         2165 g         3155 t
ORIGIN      
        1 ctactcgatt aactcccctg aaatttgttt taattatgca aatttcacgt atttttgatg
       61 ctgagacgac gatcctggga acttttcaga tatttttttg actatctaaa atctatcatt
      121 agaaaagctt agagcgccaa aggatttgag cgtttttctg atttttaaga ctttttccca
      181 gtctcttttt cgattgaaga tgtaattatt ctactaacta acttcttagt actagccaac
      241 aacgataatc ataattcctc ctaaaattag gaataataaa ggcaatagtt tttgtttttt
      301 catgtaaaaa acctcacttt tgttttctgc tattttatgc taaaatatta aaaatcaaat
      361 ttaattccaa agtttgtaac taaaggggga gcgctacatg tctaattcat ttgtcaagtt
      421 gttagtctct caattatttg caaatttagc agatattttc tttagagtaa caatcattgc
      481 taacatatac attatttcaa aatcagtaat tgccacatca ctagttccta tcttaatagg
      541 aatatcctct tttgttgcga gtcttttagt tccgttggtt actaaaaggt tagcgctaaa
      601 tagggtttta tctttatctc aatttggaaa gactatatta ttggcgatac tggtaggaat
      661 gtttaccgta atgcaatccg tagcgccttt ggtgacctat ctatttgttg ttgcaatttc
      721 catactagat ggttttgcag cacccgtttc ctatgctatt gtgccacgct atgcgaccga
      781 tttgggtaag gctaattcag ccttatcaat gactggtgaa gctgttcaat tgataggttg
      841 gggattaggt ggactcttgt ttgcaacaat tggtctgtta cctaccacgt ttatcatttt
      901 agtcttgtat atcatttcta gctttctgat gttatttctt cctaacgctg aagtggaggt
      961 gttagagtca gaaactaatc ttgaaatttt gctcaaaggt tggaagttag ttgctagaaa
     1021 tcctagatta agactttttg tatcagcaaa tttattggaa attttttcaa atacgatttg
     1081 ggtttcttcc attttgtagc tatggcaata gtgacactga ctattctata ttttccaaac
     1141 gcacagatgt ttttattatt ttcagcttta gttggtatgt tatcgcaact aaaagaagtt
     1201 cctgaaagtg tatttcttca ggaaacagta gaggaaaatc atttagttaa tgtctattcc
     1261 gttcttgaag tgatttctac attagcattt tcagtatttg ttttgctaat gagctatatt
     1321 actgagagtt ttggtattag catcagtttt tggctatcag ccatttgtct gatgatagaa
     1381 gctattttaa tttatatcag acgagattat tttaaatgag agagtggtta aaaagtcttt
     1441 aaaaatcaga aaagcgcata gtatcaggtg ttgaaaaaac ttgatattgt gtgttttatt
     1501 atgggaagat ttacttcatt gtctcctgaa atggagtttt gaagcaggat cactctattt
     1561 gaaaatatac atagaaaaac tgtataatcc ttaaaataag ctgaaatcta ttggaaacat
     1621 acggtaaaca attgaaaagg tactatataa agtggtacaa tggtagaaaa ataagctagt
     1681 aagaatcact cttattttag cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca
     1741 gagaaaagaa ttgtacctct ttcttggtcg gacagccttg tattttctta tctttctagg
     1801 gctgctttac ttctttagct atcttggtca gggtcaagga agctttatct ataatgaatt
     1861 ttaacttgaa ggagaatccc tattgtgtca aataaaccaa tagcagatat gattgaaacc
     1921 attgagcatt ttgctcagac acagcctagc tatcctgtct ataatgtttt ggggcaggaa
     1981 cacacttatg gcgatttaaa ggctgattcg gatagtttgg ctgcagtcat tgaccaacta
     2041 ggcttgcctg agaagtctcc tgtggttgtt tttggtggcc aagaatatga aatgttggca
     2101 acctttgtag cgctgactaa gtcaggtcat gcctacattc caattgatag ccattcggcc
     2161 ttggagcgag tttcagctat tttagaagta gcagagccaa gcttgattat tgccatttca
     2221 gcctttccct tggagcaggt ttctacacca atgataaatc tagctcaggt tcaagaagcc
     2281 tttgttcaag ggaataacta tgaaatcacg catccagtca agggagatga taattactac
     2341 attatcttta cttctggtac gactggtaag cctaagggag tgcagatttc acatgataat
     2401 ctcctcagct ttacaaactg gatgattatg gataaggaat ttgcgacacc gagtcgtccg
     2461 caaatgctgg cacagccacc ttattctttt gacttgtctg tcatgtattg ggcaccgacc
     2521 ttggcacttg gtggtacgct tttcactctt ccttcagtca tcactcagga ctttaagcaa
     2581 ctctttgcgg ctatcttttc attgccaatc gctatctgga catcaacacc atcctttgca
     2641 gatatggcca tgttgtctga atacttcaac agtgagaaaa tgcctggagt cacgcatttc
     2701 tactttgatg gtgaagaatt gacggtcaaa acagctcaaa aactgcgcaa gcgtttccca
     2761 aatgcccgta tcatcaatgc ttacggccca acagaagcga cagtagctct gtcagcagtt
     2821 gccgtgacag acgagatgtt agcgactctc aaacgcctac caatcggcta taccaaggct
     2881 gattctccaa cctttatcat tgacgaggaa ggaaataaac tgccaaatgg cgaacaggga
     2941 gaaatcattg tttctgggcc agctgtttca aaaggttata tgaacaatcc tgaaaaaaca
     3001 gcagaagcct tctttgagtt tgaaggtctg ccagcctatc acacaggcga tgtgggaacc
     3061 atgacagatg agggcttgct tctctacggc ggacgcatgg acttccagat taagtttaac
     3121 ggttaccgca ttgagttaga agatgtctct caaaacctca acaagtctcg ctttatcgaa
     3181 tctgctgtcg cagtaccgcg ctataacaag gaccacaagg tacaaaatct attggcttat
     3241 gtcatcttaa aagacggtgt tcgtgagcag tttgagcgag atatcgatat taccaaggcc
     3301 atcaaggaag acctgacaga catcatgatg tcctatatga tgccatctaa attcctttac
     3361 cgagacagtt tgccactgac tccaaatgga aagattgaca tcaaaggatt gattaacgag
     3421 gtgaataaga gatgatggag ttttttcaac agcttcctca tttagagcca tatggcaatc
     3481 ctcagtattt tgtttatgtg attgctgcaa ccttgcccat ctttataggt ctctttttca
     3541 agaaacgctt tgcctggtat gaagtgttgg taagtctctt ctttattgtc accatgttgg
     3601 tgggtggaaa gaccaatcaa ctagctgcct tgggtattta cctttgctgg gaaatattgc
     3661 tcctgctttt ctacaagcat tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag
     3721 ttagttttct gtccctactt ccgattatct ttgtcaaggt gcaaccagct atcaatggaa
     3781 cgcagtcttt gcttgggttc ttgggaattt cttatcttac ctttcgttcg gttgggatta
     3841 tcatcgagct gagagatgga gtgattaagg attttaccct ctgggaattc ctccgtttcc
     3901 ttctcttcat gccaactttc tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa
     3961 attatcaggc tattcctgag cgagatgagt tgatggatat gctggatgaa tctgtccgct
     4021 atatcatgtg gggctttttg tataagttta tcctagctca tgttttagga gagaccttac
     4081 tacctcctct gaagaattta gccttgcagt caggtggctt ctttaatctc tatgccttgg
     4141 cagttatgta tacttttggt ctggagctct tctttgactt tgcaggttat tctatgtttg
     4201 ctttggccat ctcaaacttg atgggaatcc gtagccctat caactttaac aagccctttt
     4261 tatcaaggga tttaaaggag ttttggaatc gctggcatat gagtctgtcc ttctggttcc
     4321 gtgactttgt ctttatgcga atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc
     4381 gtaatgtaac ctcaagcatg gcctacattg taaatatgct gattatggga ttttggcatg
     4441 gtgtgacctg gtactatatc gcctatggac tctttcatgg actaggcttg gtcatcaatg
     4501 atgcctgggt tcgcaagaaa aaaacactca ataaggaacg gaaaaaagca gggaaggctg
     4561 ccctacctga gaatcgctgg attcagttgc ttggcatggt tgtcactttc catgttgtca
     4621 tgttgtcatt cttaatcttt tctggattct tgaataatct atggtttaaa aaataaaagg
     4681 aaataaaata tggatatcaa atcagaagtt atcgaaatta ttgatgagtt gtttatggaa
     4741 gatgtttctg acatgatgga tgaagatctt tttgatgcag gtgtcttgga tagtatggga
     4801 acggttgagt tgattgtgga gattgagaac cgttttgaca ttcgtgtccc tgtaacagag
     4861 tttggtcgtg acgactggaa tacagctaat aaaatcatag ctggtattgt ggagctacaa
     4921 aatgcttaaa cgcttatgga tgatcttcgg accggtcttg atcgctggtt tgttggtttt
     4981 tctgctcatt ttcttttatc ctactgagat gcatcataat ctaggagctg aaaagcgttc
     5041 agcagtggct actactatcg atagttttaa ggagcgaagt caaaaagtca gagcactatc
     5101 tgatccaaat gtgcgttttg ttcccttctt tggctctagt gaatggcttc gttttgacgg
     5161 tgctcatcct gcggtattag ctgagaaata caatcgttcc taccgtcctt atcttttagg
     5221 acagggggga gctgcatcgc ttaaccaata ttttggaatg caacagatgt taccacagct
     5281 ggagaataaa caagttgtgt atgttatctc acctcagtgg ttcagtaaaa atggctatga
     5341 tccagcagcc ttccagcagt attttaatgg agaccagttg actagttttc tgaaacatca
     5401 atctggggat caggctagtc aatatgcagc gactcgctta ctgcaacagt tcccaaacgt
     5461 agctatgaag gacctggttc agaagttggc aagtaaagaa gaattgtcga cagcagacaa
     5521 tgaaatgatt gaattattgg ctcgttttaa tgaacgccaa gcttcctttt ttggtcagtt
     5581 ttcggttaga ggctatgtta actacgataa gcatgtagct aagtatttaa aaatcttgcc
     5641 agaccagttt tcttatcagg caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac
     5701 ttccaataat gagatgggaa tggaaaatta tttctataat gagcagatca agaaggattt
     5761 gaagaaatta aaggattctc agaaaagctt tacctatctc aagtcgccag agtataatga
     5821 cttgcagttg gttttaacac agttttctaa atctaaggta aacccgattt ttatcattcc
     5881 acctgttaat aaaaaatgga tggactatgc tggtctacga gaggatatgt accaacaaac
     5941 ggtgcagaag attcgctacc agttagaaag tcaaggtttt accaatatag cagatttttc
     6001 taaggacggc ggggagcctt tctttatgaa ggacaccatt caccttggtt ggttgggttg
     6061 gttggctttt gacaaggcag ttgatccttt cctatccaat cccacaccag ctccgactta
     6121 ccatctgaat gagcgctttt tcagcaaaga ttgggcgact tatgatggag atgtcaaaga
     6181 atttcaatag ataatataat atagaagagt tcaagaatga aactagtttt cacgtttttt
     6241 cttgactctt tttttgcttg tgatataatt aactggtaaa caaaatgtca aagaatagta
     6301 tttttctctt aaaaaagaga agtctaaaag gaaaggagtc agatatgaga cagctagcaa
     6361 aggatatcaa tgcttttttg aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc
     6421 taataggtca ttgcactagc gaggtggccc tgaccaatac tcaggagcat atccttatgc
     6481 tcttgtcaga ggaatcttta acaaattcag aattggcccg tcgtctcaat gtcagtcagg
     6541 cggcagttac caaggccatt aagtctttgg tcaaggaagg gatgttggaa acatctaaag
     6601 attctaaaga tgcgcgtgtg attttttatc agttgactga cttggctcgt ccaatcgctg
     6661 aggagcacca ccatcaccat gagcatacac ttttaaccta tgaacaagtg gcgactcagt
     6721 ttactccaaa tgaacaaaaa gtgattcagc ggtttttgac tgctttagta ggagaaatca
     6781 aataatgaga tatattacgg tagaggattt gtccttctat tatgataagg aacctgttct
     6841 tgaacatatc aattattgtg ttgatagtgg ggaatttgtt accttgactg gggaaaatgg
     6901 agcggctaag acgacgctca tcaaggccag tcttggaatt ctgcaaccac gcattggaaa
     6961 ggtggctatt tcaaagacaa atacgcaagg taagaaattg agaatagcct atcttcctca
     7021 acaaattgcc agttttaatg ctggttttcc aagtacggtc tatgaatttg tcaagtcggg
     7081 tcgctatccg agaaaaggct ggttccgtcg tttgaatact catgatgagg agcatatcaa
     7141 ggctagtctg gactcagttg gcatgtggga acatcgagac aaacgcttgg ggtctctatc
     7201 tgggggacaa aagcagcgag cggtaattgc gcgtatgttt gcttctgacc ctgatgtgtt
     7261 tatcctagac gagccgacaa cggggatgga tgcaggaagt aaaaatgaat tttacgaact
     7321 catgcaccac agcgcccatc atcatggcaa ggctgttttg atgattaccc atgaccctga
     7381 agaagttaag gattatgcgg atcgcaatat tcatctagtc cgtaaccaag actcgccatg
     7441 gcgttgtttc aacgttcatg agaatgggca ggaggtgggc catgcttagt ttattatctt
     7501 acgactttat acaacgcgcc tttttggcgg ttattgctat gagtcttttc tcaccggtat
     7561 tgggaacctt cctcatcttg cgtcgtcaga gtttgatgag tgataccctt agccacgtct
     7621 cactttcagg tgtagccttt ggtctggttt tggggatttc tccaactgtt tctactattg
     7681 ccattgtctt gattgcggcg gtctttctgg agtatctccg tacggtttac aagagcttta
     7741 tggaaatcgg gacagctatc ctcatgtcaa caggtttggc tgtttctctg attgtcatga
     7801 gcaagggtag aagctcgagt tcaatgagtt tggaccaata tctctttggt tcgatcgtga
     7861 ctatcagtga agaacaggtc atttccctct ttgtcattgc ggcggttgtt ttgattttga
     7921 cctttctctt tcttcgtcct atgtatatct taacttttga cgaagatacg gcctttgtgg
     7981 atggcttgcc agttcgtacc atgtccattc tttttaacat ggtgacaggg gtggctattg
     8041 cccttatgat tcctgcagca ggagctcttc tggtatcgac cattatggtc ttgccagcta
     8101 gtattgccct gcgtctgggg aaaaacttta aatcggttat gctgcttgcc agtgcgattg
     8161 gctttttggg aatggtagca ggactttaca tttcctacta tgcagaaaca cctgcaagtg
     8221 caagtattac cattattttt gtaactgtct ttatactaat cagtttagta agacgtttta
     8281 tcaaatagga gacgaacgtg aaaaaaatta gcttattact agccagtcta tgtgccttgt
     8341 ttttagtggc ttgttccaat caaaaacagg cagatggtaa actcaatatc gtgacaacct
     8401 tttaccctgt ctatgaattt accaagcaag tcgcaggaga tacggctaat gtagaactcc
     8461 taatcggtgc tgggacagaa cctcatgaat acgaaccatc tgccaaggca gttgccaaaa
     8521 tccaagatgc agataccttc gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat
     8581 tgctagatac cttggataag aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc
     8641 tcttgccagg tggcgaggaa gaagagggag accatgacca tggagaagaa ggtcatcacc
     8701 atgagtttga cccccatgtt tggttatcac cagttcgtgc cattaaacta gtagagcaca
     8761 tccgcgacag cttgtcagca gattatcctg ataaaaaagt gacctttgag aagaatgcag
     8821 ctgcctatat cgaaaaattg caagccttgg ataaggctta cgcagaaggc ttgtctcaag
     8881 caaaacaaaa gagctttgtg actcaacacg cagcctttaa ctatcttgcc ttggactatg
     8941 gactcaaaca agtcgcaatc tcaggacttt ctccagatgc agagccatca gctgctcgct
     9001 tggcagaatt gacagagtat gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa
     9061 atgcctcaca agcccttgct aacacacttt caaaagaagc aggtgtcaaa actgatgtcc
     9121 tcaatccttt agaaagtctg acagaagagg acaccaaggc tggagaaaac tacatttccg
     9181 tgatggagaa aaacctcaag gctttgaaac aaacaacaga ccaagaaggc ccagcaatcg
     9241 aacctgaaaa ggcagaggat accaagacag tccaaaatgg ttacttcgag gatgcagctg
     9301 tcaaggaccg caccttgagt gactatgcag gtaactggca atcagtttat cctttccttg
     9361 aagacggcac gtttgaccaa gtctttgact acaaggctaa gttgactggt aagatgaccc
     9421 aggctgagta caaggcttac tatacaaaag gctatcagac agatgtgact aagattaaca
     9481 ttactgataa tactatggaa tttgttcaag gtggacaaag caagaaatac acttacaagt
     9541 atgtcggtaa gaaaattttg acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg
     9601 aagccacaga tgctgacgct ggacaattca agtatgttca gtttagtgat cacaatgttg
     9661 ccccagttaa ggcagaacat ttccatatct tctttggagg cacaagccaa gaagccctct
     9721 ttgaagaaat ggacaactgg ccaacctact acccagataa cctatctggc caagaaatcg
     9781 cccaagaaat gttggcgcat tgatgagaaa tcgactagtt cataagagct agtcggtttt
     9841 ttgatactag atggctaact tcattataga cctttcagaa ctttaaacaa taaataaaac
     9901 tcttgaaata tctgggctta tatgctagaa tgtagttagt gtataaggtt cattagaaca
     9961 ccaaagcccc taactagtgc catagttaag ggcttttttg acgtatctta gcgatttaac
    10021 gggtgtttat aggctagtca gtcggtctac ttcttaccat ctagccattt gcagacgaaa
    10081 tacaagataa ttccagctat aacatctgct ataatagtaa gagcaagctc tgg
//
LOCUS       DAVBCV010000069         9629 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBCV010000069 DAVBCV010000000
VERSION     DAVBCV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.069"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            363..950
                     /locus_tag="VQ516_001845"
     CDS             363..950
                     /locus_tag="VQ516_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512238.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGTLLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQKKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(1037..1240)
                     /locus_tag="VQ516_001846"
     CDS             complement(1037..1240)
                     /locus_tag="VQ516_001846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512239.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(1537..2241)
                     /locus_tag="VQ516_001847"
     CDS             complement(1537..2241)
                     /locus_tag="VQ516_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2512240.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(2311..4137)
                     /gene="glpO"
                     /locus_tag="VQ516_001848"
     CDS             complement(2311..4137)
                     /gene="glpO"
                     /locus_tag="VQ516_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2512241.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            complement(4179..5687)
                     /gene="glpK"
                     /locus_tag="VQ516_001849"
     CDS             complement(4179..5687)
                     /gene="glpK"
                     /locus_tag="VQ516_001849"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2512242.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(5846..7273)
                     /locus_tag="VQ516_001850"
     CDS             complement(5846..7273)
                     /locus_tag="VQ516_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2512243.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            7438..8310
                     /gene="hslO"
                     /locus_tag="VQ516_001851"
     CDS             7438..8310
                     /gene="hslO"
                     /locus_tag="VQ516_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2512244.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQERHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            8297..9277
                     /gene="dusB"
                     /locus_tag="VQ516_001852"
     CDS             8297..9277
                     /gene="dusB"
                     /locus_tag="VQ516_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2512245.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(9507..>9629)
                     /locus_tag="VQ516_001853"
     CDS             complement(9507..>9629)
                     /locus_tag="VQ516_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512246.1"
                     /translation="KASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
BASE COUNT         2742 a         2186 c         1922 g         2779 t
ORIGIN      
        1 aatacgtcta tttatttttt attgtttttt attgtagagc agacccaagc gaatgaacat
       61 aggttgtaaa aagcaaatga aaaaactgcg aggataatag cttctgtgtc taaataaccc
      121 atttcgagac ctcttttcaa caacaacaag cctgtagttt cgataacgat caaacataaa
      181 aatatataaa aaattttcgc attaagcata ttgttaccgc tttcttttta tttggtttta
      241 ttgtataata aaagttgaaa aaaatcaaaa atacaaagga gtttccgtta tgtctaagat
      301 gtttaaatgc atgtgtttgc tagttatgtc tgttttctgt atgtctagca tttccccgtc
      361 gcttgtttgt ttttgctgat gatagcttat cggcaaactc taaagttgta tctggtgagg
      421 ctcagtttga gaatggctca tcagtgcggt tcggagatac gcaagtaaat attttaagcg
      481 atgaagttct tgaggttgtc aatccagatg gaagtgtaga cactattgaa cgacgtgctg
      541 acggcgtcta tatcaatggg gcattttata tggcttatca aaagaatgaa atcgacctaa
      601 atatatcttt tagatcatac gatcctaatg tttggaatta cgtcaacaca atacatggga
      661 ataaacaagc taacacattc gctaacttca tgactggtgc tggaatcagt tatatgattg
      721 gtaggatagg tactttattg gggggtccat ggggtgcaat aattggtgga gcatattttg
      781 gtatacaagc ttatcagtct tacttggatt cacaaagccc atatccatac tacataacaa
      841 gtacttatat ccatgttgct caaaaaaaat ggaagtttat cactgaatac tatagaaact
      901 caaactatac tgggtatgta aaaaccgtaa caacctacgt gaatttctaa aatataaacc
      961 ctgcctagat ataaaggcag ggtttatatt ttagttaatt gctcaagtaa tttgtaagca
     1021 taatacttgc gaaactctat ttgttcaagc ggttcaaaag ctcttgtttt ttatcctcaa
     1081 gctcagctct cctttgctcg atttcttcac gtttttgggc gatttctgct tgcgcctcgg
     1141 gagtttccaa agtgccttgt gacttgtgaa attctactag catatcaacg atttttgggt
     1201 ctaattcgtt gacataatca ggatttgacc atttcggcac gttgcttgca ggtgcttgct
     1261 ttttgccgcc ttttttgggc gttttggctt gttgttcagc tttctgactg tagttgacct
     1321 cgtggaattt tcgtgcttga tatgatgcgt tttattatga gaagatttac ttcatttttt
     1381 gctgaaattg ggctcttgcc caagctctat caacataatt gttttctaat actcaatgaa
     1441 aatcaaagag caaactagga agctagccgt aagctgtact tgagtacggt aaggcgacgc
     1501 tgacgtggtt tgaatttgat tttcgaagag tataaactag aaaagtgaga atacaagcac
     1561 tgccaaggct gctccgataa cagggcctac aacaggaatc caagcgtaag accagtctcc
     1621 gtctcccttg tttggaattg gcaagatgct gtgcatgata cgaggtccaa ggtcacgagc
     1681 tgggttcaag gcataacctg ttgtcccacc tagtgataga ccgataccga caatcaaagt
     1741 tcccactgca aaggttccga tacctgcctg aaaatcgtaa agacccaaag caaagattgt
     1801 caacaccaaa acaaaggttc caaggatttc gctaatcaag tttgatacag tatccttgat
     1861 ggctggtcca gtactgaagg ttgccaggat attgcctgca ttttcttctg cctcatagtg
     1921 aggtttgaat tgcaaccaaa ccaaaatctg acccagcatg gcccctgcga actgggctaa
     1981 gatataaggc aaaacggaag cccaaggcaa accacctttt aaggccacac cgatggtcac
     2041 agctgggttt aaatgagctg gactgagctt gccagataca aagactgcaa ccgcaactgc
     2101 aatcccccaa cccatagtaa tcacaatcca acctgagcta ttgctcttgg ttttaggaag
     2161 aaccacacct gcaacaacac catttcctag aagaatcagg attaaagtcc ctagaaattc
     2221 tccaaataat tcattcatca tctttctgtc tccattaaaa agaagggacg ggcgacaagg
     2281 aatgctgccc tccacctctt ttattttttc ttaatttttt aattctgcta aatcgttgtt
     2341 agcgagagct gcttcgacat cagcacggta agttgctttt tcttcttctg tccagtcata
     2401 gaatcgtccc atttcatcca aaattggctc aacgatgcta tccaagctat cacgcataaa
     2461 gagcatatgg ttggtacgac gaagaaggaa gtcaactggg ctaagagcca actcgttgcg
     2521 cattgcatag tgaagggaca aagtatctgc caagctgagt cctggcgctt gttccaagct
     2581 gtgagcaagt gcaaagactt tcggtgcatt tgaaccgtaa agatttgcca gatagtgagc
     2641 ttccttgcta tccaaaccac gtgatactcc aagttgcgca aaggcttcga tttctgaatc
     2701 cacatttgct gggttcaatt ctccacctga aacagggtaa gttttagaat tgatcaattt
     2761 aaagctacgg tcaaattctg ctttgaggat gtcaaccacg cgctccatag ctccttcagc
     2821 catcttacgg tagtctgtga ttttaccgcc agcaagagtc aaaagaccat tgtcatcacg
     2881 gtccaagcta gacccacgag aaactgcaga tggatccaaa tgtttctcag atgtgctact
     2941 ttcaagcttg ctgacagcag actcaacatc ttcacgtgtt ttttctttgg agagataaga
     3001 ttcaacagtc gcaatcaagt tgtcaaagct ttcatcactg atggtaccgt tatttccacc
     3061 attatagtca gaggcactgt tccctgcaat caatggacga agacctgccc agctgctttc
     3121 gatatcatca atggtgatgt tggattctgg aaagcggttg ttgacaatgc caagtagata
     3181 atctacatct tcttgagtta cttttggatg ctccaaatca cctgtgtagt ctgtatcagt
     3241 tgtaccaaag taagtcttgt tttcacgtgg gagaacaaag accatacgac cgtcacccaa
     3301 acctgtgtca aagtaaactg gctgtgaaac cttgattttg cttgaatcca ctaccaagtg
     3361 aactccctta gttgggcgca tttgtgagaa ttgcgttccc ttattagaca aattacgtac
     3421 tttatcactc caaggacctg ttgtattaat aaccagacgg gccttgattt caaatacttg
     3481 gtctgtcaag agatcacgag ctacaacacc tgtaatcttg ccactttcgt caaagaggaa
     3541 gccttctgcc ttcacgtggt tggcaatgag ggcaccgtct tggttggcac gtttgatgtt
     3601 ttcaatcacg agacgcgcat cgttgttacg gaagtcaaga tacactccac ctcctaccaa
     3661 gccttccttc ttcaagtttg gctggcgttc caagacttga tccttgctca aaaccttgtt
     3721 cgcagttggt gtgttgctaa cacctgccaa gaggtcgtac aagtccatgg ctactttaag
     3781 acggaagagg ctaaaggttg ctccatcttc atcgtaaact ggtaagagca ttgggtctgg
     3841 ttttggaatg tgtggagcga tttgttgaac cactgcacgt tcagaaaccg tatctgagac
     3901 cacttctacg tcgaattgtt taaggtaacg aagtcctccg tgaaccaatt ttgttgaacg
     3961 actagatgtt ccttctgcaa agtcttgcat ttcaatcaaa ccagtctcaa gaccgctagc
     4021 tgccgcctgc aaggctacac cagctcctgt gattcctcca ccgataatca agaggtccag
     4081 ggtgcgttcc tgcatttttt taattgacaa ttcacgtgtt ttctttgaaa attccatatt
     4141 tacacacttt ctaaataaat cgctttattc tgccagtatt agtcgtctac ttccgcaaag
     4201 acttgagttg ctttcacagc cttcttccag cccttgtaga gttgttcctt gcgagattcg
     4261 ttcatagatg gctcaaagag ttctcctgtc tcgttcaaga gtttcaactc gtccaagtct
     4321 ttccagtacc ctactgacaa acctgctagg aaggccgctc ctagagctgt tgtttccagg
     4381 tttttagcac gtgcaatgtc aatgcctaaa atatccgcct ggaactgcat gaggaagttg
     4441 ttcatggctg caccaccatc caccttcagt acttgaatgg cggtctgagt atccacttgc
     4501 atggtgtcga tgatatcacg cacttgataa gcaatagatt gcaaagtcgc cttgataaag
     4561 tcttctttgc ttgttccacg agtcaaacca aagacggaac cacgagcatt ttggttccag
     4621 tatggagcgc ctagacctgt aaaggctgga acgacataaa cttcatcgtt gttgtgagaa
     4681 tcacgagcgt atttttcaga ttctggtgaa ttttcaacca tgcgaagacc gtcacgaagc
     4741 cactgaatag cacttcctgc gatgaagata gaaccttcca aggcataata aaccttaccg
     4801 ttgattccgt aaccaatggt tgtcaagagg ttgttttcag acaactgcat ctcttcccca
     4861 gtattcatga tgatgaaaga gcctgttcca taagtattct taaccatacc tggctcaaaa
     4921 gccaactgtc caaagagggc tgcttgttgg tccccagcca tacctgagat tggcacctct
     4981 ccaccgtaga aatggaatgg agctgtcttg ccgtagattt cggagttaga acgaacttct
     5041 ggaagtatag ccttcggaat gttaaggatt tccaaaatct catcatccca tttgagttct
     5101 ttaatgttat aaagcatggt acgagctgca tttgagtagt cagtcacgtg agccgcaccg
     5161 tcagtcaatt tccaaaccaa ccaagtatcg atagtaccaa agagcaattc ccctttttct
     5221 gctcgctctt gagcaccttc tacatgatcc aaaatccaac gaaccttggt agcagagaag
     5281 taagcatcaa taatcaaacc agtcttttca tggaattttt ccacataacc ttggcttttt
     5341 agttgctcag ccaaaggtgc tgtctggcgt gactgccaaa cgatagcatt gtagatagga
     5401 agtcctgttt tcttatccca gacaaccgtt gtttcacgtt ggttggtaat cccgattgcc
     5461 tcgatttgat ttggcttgac accactttcg atgaaagcac ccgcaataac tgactgaaca
     5521 gagttccaaa tttcattggc attgtgctca acccaacctg cctgagggaa aatctgggta
     5581 aactcttttt gactcgagct aaccttttcc ccttttttgt tgaaaatgat ggcacgagaa
     5641 cttgtagttc cctggtcaat ggccatgatg tatttttctt gtgacatgta ctgtcctcct
     5701 gataaagatc ttgaggtgtt tcctctttac acttactagt atacaaaata aagcgctttc
     5761 ttgtttatca acttttttta atttttaaaa aaatgtgagg agattgtgtg aatttcacaa
     5821 aaaagaccta gattaaccaa gccttttaag tgaataataa ctgacgaatc cctgccaaat
     5881 cttccatatc caagtcgttt tttaaataat agactggtgt ctgttccttc ttatgaatcg
     5941 ggttattggt aaccagcaaa tcataatgcc gactagggtc ataagcttca atggtaataa
     6001 aacgattgta ttccaaatac cgtttcaaaa tggctgccat ccttgaaaag acaagaaaac
     6061 cagatgtcaa atccagacca atccgcatat gctggccacc gttttcagcc atatattcga
     6121 ttaactggag ccattcccag agaatcttct tatctaaatc tgtcccctgt tgaaaagcag
     6181 gtagagcatg aaaaatctcc tctgctgtct ctttaaaatc atgttccatc agtaaatatg
     6241 gatgacgatt ctctaactgg tacttgtagc gatcctgtaa aatatagccc ttatagagat
     6301 agacttgagc acaaagctga ctgagttcat aggtgacatg gtcctctgta taatccccca
     6361 atagttcctc cttcttcatt tcttgaatca attgcgtcag taaatctgcc aactgccctc
     6421 caaaaccaag aatatactcc atagtatgaa gaggaagaat gcgatgagat aggagaaaag
     6481 agaagaatat catcatctca ccatcctcaa ctcctagtgg aatatgttgc ccagcaaaaa
     6541 aggacggaac ctttctcagc aaacgaagat aaaagatatt gtcaaaatac cctcgcattt
     6601 tctcttctat gacttgaaac tgacaagcat tgacccgaag acgttgttga ctgatgtgag
     6661 cccagagaac caagtccaat ttctgccccg cagacaaact tgcaccgcag atttcctcta
     6721 aattggcaat ctcctgtttt ctctctggtt tctgcatgtg accttcccat tcctgactcg
     6781 accagacctt tcggaaaaga cagaaataga aatagcgaat ctgatgctct ggacctctcc
     6841 agcgtccatt ttggatggat aaatcaaatt ctgacaaaat ctgatttaaa ccagccaagt
     6901 gacgaccaag cgtagcctcg ctaatcacca attcttgagc cagctgatgg gctaaaaact
     6961 gttggtggta gagaagataa accaaaatct ggtatttaac agcactatcc aaaaacaagc
     7021 tccgaatatc tctccccttg gtagctgcac cgatagacag acgcagattt tcatcttctg
     7081 agtgaatagt cagttctaag ccactatcca aagccttgtc attgagcagg gtgacatatt
     7141 tggttagggt tgcttttgaa aatcctgttt cttccattac agccttgacg gtcgtctgag
     7201 actcttgtaa tagaaaggaa aggattgaaa attgaccaca ctcggctttc tccatcaaat
     7261 ctcctaaata catttgttac ccctttcatt ttctacctta agcatagcat aaatcttaca
     7321 aatgctgaaa taatctgttt ctctttttat tttcatgctg atttcctggt ccattatcct
     7381 gaaaatcagt aaacacacgg ctcccccttt gggcattttt atgctaaaat agtagctatg
     7441 gataaaatta ttaaaactat atcagaaagc ggagcctttc gtgcttttgt ccttgatagc
     7501 acggaaaccg tccgcactgc tcaagaaaga catcaaaccc aagctagctc aactgtagcg
     7561 cttggtcgaa ctcttatcgc tagccagatt ctcgcagcca atgaaaaagg aaataccaaa
     7621 cttacagtta aggtgttggg ctctagctct ctaggtgcta ttatcaccgt cgctgatacc
     7681 aaggggaacg tcaaaggcta tgttcaaaat cctggtgttg acatcaaaaa gactgcgact
     7741 ggtgaagtcc tagtcggacc ttttgttgga aatggtcaat tcctcgttat cacagactac
     7801 ggtgctggaa atccttacaa ctctataact cccctcatct ctggagaaat cggtgaagac
     7861 cttgcctttt accttactga aagccaacaa acgccttcag cggtcggcct caatgtcctt
     7921 ttggacgagg aagacaaggt caaggttgca ggtggtttcc tagttcaagt cttgccagga
     7981 gccaagaaag aagagattgc tcgctttgaa aaacgcatcc aagaaatgcc agctatctct
     8041 actcttctcg aaagcgacga ccatatcgaa gccctcctca aggctatcta cggggacgaa
     8101 gcctacaagc gtctttctga agaagaaatc cgtttccaat gtgactgtag ccatgaacgc
     8161 tttatgaacg ctcttgccag ccttccaagc tcagacttac aggaaatgaa agaggaagac
     8221 cacggggcag aaatcacttg tcaattctgc caaactactt acaactttga tgaaaaggac
     8281 ctggaggaac tcattcgtga caaatcttaa tacacctttt atgattggca atattgagat
     8341 tcccaatcgt accgttttag cgcctatggc tggcgtgacc aactcagcct ttcgtaccat
     8401 cgcaaaagag ctcggagctg gactcgttgt aatggaaatg gtctctgaca agggaatcca
     8461 atacaacaac gaaaaaaccc tgcacatgct tcatatcgat gagggcgaaa accctgtctc
     8521 tatccaactt tttggtagcg atgaagacag cctagcacgc gcagcagaat tcatccaaga
     8581 aaacaccaag accgatatcg tcgatatcaa catgggctgc cctgtcaaca aaatcgtgaa
     8641 gaacgaagct ggagctatgt ggctcaagga tcctgacaag atctactcta tcatcaacaa
     8701 ggtccagtct gtccttgata tcccacttac tgtcaaaatg cgtaccggat gggcggaccc
     8761 atctcttgca gtagaaaatg ccctcgctgc tgaggctgca ggtgtttctg ccctcgccat
     8821 gcatggccgt acccgtgaac aaatgtatac tggccacgca gaccttgaga ccctttacaa
     8881 ggttgcccaa gctctaacca agattccatt catcgccaac ggtgatatcc gtactgtcca
     8941 agaagccaag caacgcatcg aagaagttgg tgctgacgca gtcatgattg gccgagctgc
     9001 catgggaaat ccttacctct tcaaccaaat caaccattac tttgaaacag gagaaatcct
     9061 acctgatttg acctttgaag acaagatgaa gatcgcctac gaacacttga aacgattgat
     9121 taacctcaaa ggagaaaacg tcgcagttcg tgaattccgc ggcctcgctc ctcactatct
     9181 ccgtggaaca tctggcgctg ccaaactccg tggagccatt tcgcaagcta gcaccctggc
     9241 agagattgaa gccctcttgc aattggagaa ggcttaatag tttaaaaccc gtaactctct
     9301 taaagagcct cttgaatgcc gccattcttt cctcatcaaa aaaactccgt agtctctagc
     9361 gctataaatc cggctagaga ttacagagtt atttttttat ctaaacttcc tacaagggta
     9421 tattatctac tgaaaggata tggtatttgt agggaatact ttaaagagtt cttacaggaa
     9481 gtcagtatta actagttata ttaggtttag tttacccatt caccattggc attgactcta
     9541 tagctatcta cagttgtgtt gactgcaagg gcacctgagc cattgacata gtaccattta
     9601 tctgatactt tgaaccattg gcttgcttt
//
LOCUS       DAVBCV010000070         9472 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBCV010000070 DAVBCV010000000
VERSION     DAVBCV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.070"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            150..686
                     /locus_tag="VQ516_001854"
     CDS             150..686
                     /locus_tag="VQ516_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2512247.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            737..823
                     /locus_tag="VQ516_001855"
                     /pseudo
     CDS             737..823
                     /locus_tag="VQ516_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(892..1107)
                     /locus_tag="VQ516_001856"
     CDS             complement(892..1107)
                     /locus_tag="VQ516_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2512248.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            1205..1525
                     /locus_tag="VQ516_001857"
     CDS             1205..1525
                     /locus_tag="VQ516_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512249.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(1556..1894)
                     /locus_tag="VQ516_001858"
     CDS             complement(1556..1894)
                     /locus_tag="VQ516_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512250.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(1906..2625)
                     /gene="trmD"
                     /locus_tag="VQ516_001859"
     CDS             complement(1906..2625)
                     /gene="trmD"
                     /locus_tag="VQ516_001859"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2512251.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(2615..3133)
                     /gene="rimM"
                     /locus_tag="VQ516_001860"
     CDS             complement(2615..3133)
                     /gene="rimM"
                     /locus_tag="VQ516_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2512252.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKRYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(3164..3952)
                     /locus_tag="VQ516_001861"
     CDS             complement(3164..3952)
                     /locus_tag="VQ516_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2512253.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(4529..4768)
                     /locus_tag="VQ516_001862"
     CDS             complement(4529..4768)
                     /locus_tag="VQ516_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2512254.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(4788..5060)
                     /gene="rpsP"
                     /locus_tag="VQ516_001863"
     CDS             complement(4788..5060)
                     /gene="rpsP"
                     /locus_tag="VQ516_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2512255.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            5230..5439
                     /locus_tag="VQ516_001864"
     CDS             5230..5439
                     /locus_tag="VQ516_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512256.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            5918..6139
                     /locus_tag="VQ516_001865"
     CDS             5918..6139
                     /locus_tag="VQ516_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512257.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            6222..7025
                     /locus_tag="VQ516_001866"
     CDS             6222..7025
                     /locus_tag="VQ516_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2512258.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(7081..8622)
                     /locus_tag="VQ516_001867"
     CDS             complement(7081..8622)
                     /locus_tag="VQ516_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2512259.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(8624..9106)
                     /locus_tag="VQ516_001868"
     CDS             complement(8624..9106)
                     /locus_tag="VQ516_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2512260.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(9106..>9472)
                     /locus_tag="VQ516_001869"
     CDS             complement(9106..>9472)
                     /locus_tag="VQ516_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET2512261.1"
                     /translation="KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNI
                     KKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQ
                     LLFNTMKRDRQKAVAAVNP"
BASE COUNT         2796 a         2028 c         1698 g         2950 t
ORIGIN      
        1 attttcgaat caacaaaaaa attatccaac ttgactagtc acgaaaatcg gctgttacag
       61 tttttgttac cttgttttta aaaaatggtt gacatcgact cctatatgag tataatagtt
      121 atcatacatt agaaaagagg ttaactattt tgaaaaaagc tcacgtttat gctatccctg
      181 ctattggggc tgctctcatt gctgctttgg cacaaatcag tcttccaatt ggacctgttc
      241 ccttcactct gcaaaacttt gcaatcggct tgattgctac tgtctttaga ccgagagagg
      301 ctgtactttc tgctggactc tatcttcttc taggtgctat cggtcttcct gtctttgcag
      361 gaggtggagc tggttttcag gctttagttg gccctactgc aggctatctt tggttttatc
      421 tcgtttactc tggacttact tcctctctaa ccaacagcaa gagtggtgtt gttaagattt
      481 ttcttgcaaa cctcttgggt gatgcccttg tctttgtcgg cgggattctc agcttgcatt
      541 tcctagctgg aatggcattt gaaaaagctc ttgctgtggg ggttcttccc tttatcattc
      601 cagaccttgg caaacttctg gctattagtt ttattagccg tcccctactt caacgcctta
      661 aaaatcaggc ttactttact aactaaaaaa ggatatcgag ttatcatgac tcaatatcct
      721 tttcttttat tttgaaaact tatactcaat gaaaatcaaa gagcaaacta gaaagctagc
      781 cgcaggctgc tcaaaacact gttttgaggt tgtggatgaa actgacgaag tcagtaacca
      841 tacatacggc aaggcaaagc tgacgcggtt tgaagagatt ttcgaagagt attagttgcc
      901 tttaaaggca tccaccatcg tttgaaattc ttcatttgag agagtaatcc ctttgcccat
      961 tttagtatgg tctggactcc aagcacgaat atcaaacttt gcaggggcac cattaaagct
     1021 cacacggtta atttccttgg tccaaccttt ttcgttttca gaaagagtca acaagtgctc
     1081 ttcgatttca aatgtaaatt ctgccatttt cttctccttt tttagtttca ttagtttatt
     1141 cgtaaaatct tgtagatttt aggaaaattt tatataatat tgatataaaa gaagggaggc
     1201 caatatgaga cataaattcc agcaagttct aaataaaata catgattttt taaatggata
     1261 tgaccaacct gaccagactg aaaccaactc ccttacagcc actattgaag aggctatcca
     1321 gaaacaaacc gctgttcacc ttatcttgtc tgagacaagt tttacaggtg atatcatcaa
     1381 atatgatcag caaggccagc aaattatcgt gaaaaatttt gccaaaaatg tgagccggat
     1441 tatccgtata agcgatattc aacgcctgcg atttgtcccc tcaactgtcc aaacagccca
     1501 aaaaaataga tttaagaaag agtgagatgt agttgcttca tcccactctt ttttcttagc
     1561 gaatttgttc aaaatgtaaa tgaactgcaa tatgatctcc ataaccactt ctttccaagt
     1621 cacgttgtaa acgataggaa atgtagtgtt ctgcaatggt aatgtaacct gcgcccaata
     1681 aacgatgttc aaccatagat tgaatcatac tgatagtcgc acgttccacc ttggcttctt
     1741 gtaaatccaa aactaccttc ttagtgactt gagcaagatt ttgacgcaaa tcatctgtca
     1801 aaacataaac agtttgggct gccttcaaga tggcttggta aatcttatct ggattaaatt
     1861 cagcaatttc gccattacgt ttgattactt gcataggttt ctcctttatt ctttgttttc
     1921 tttgatttct gccagcattt tttcttcttc tactgtcagt tgataatgtt caagtaaatc
     1981 cggtctgcgc tcgtaggttt tctttaaact ctcgtacaat cgccactgac gaatcttttc
     2041 atggtggcca ctcatcaata catctggcac gaccatgcct cgataatcat agggacgtgt
     2101 gtactgagga tattctaaaa gacctgaaga aaaactatca tcttggtggc tagactcctt
     2161 gccaatcact tctggaatca ggcgaactgt agcatcaatc atggtcatag ctgccaattc
     2221 tccaccagtg aggacatagt cgcctaggga aatctcatct gttaccaagg tcttaatgcg
     2281 ctcatcataa ccctcatagt gcccacagat aaagattagc tcttcctctt gagccaaatc
     2341 ttcagcataa gcctgatcaa actgctttcc agcaggatcg aggagaataa cgcgcggatt
     2401 tttcttttca atagcatcaa aggaatcgaa aataggttgt gctctgagca acatgccctg
     2461 accgcctccg tagggctcat catctacatg acgggccttt tcagcatttt ctcgaaaatt
     2521 atgatactgg atatccaaga gccctttttc tcgagccttt ccaacgattg agtgctccag
     2581 tggagaaaac atctctggaa agagggttaa aatatcaatc ttcatcgtct aacccttcta
     2641 agatttccac atcgacccgt ttatttggaa tatcaacatt gagaaccact ggtgggatat
     2701 aaggtaaaag caaatcacgt ttgccttttc gtttgaccac ccagacatca ttagcacctg
     2761 gttgcaggat ttccttgatg gttccaacca agctatcacc ctcatagact tccaaaccga
     2821 taatctcgtg atagtaaaat tcaccatcgt ctaggtcatt caaatcttcc tcagcgacct
     2881 tgagactata tctcttgtac ttttcgatag tattgatatg gtacatatct ttgaatttaa
     2941 taatgtcaaa gttcttctgt ttacggtggc tagcgatggt cactgtttgg acaaactgat
     3001 ctttttcatc aaacaaagcc agctcagctc cttttttaaa ccgttcttct gcaaaatccg
     3061 tcacagacaa gactcgcatc tcaccctgta atccctgcgt attaacgatt ttcccaacat
     3121 taaagtagtt catcttgtct cctgtaatct ccttttttcc atcttattct gacaattctc
     3181 gaataatagc cgcaattttt tccgattctg accattgtaa ataatggtga ttccctccta
     3241 aaatgagttt agtattggaa gtccaatatt ctgattctct gtactctttt tctctataag
     3301 gctgacaaaa aacaaataca ggaatatgag cttctataga tacatcctca aaatcttcct
     3361 cagtaatctc tccagatatc tgaaattctg gatcttgatt ttccaactct aagccttttt
     3421 cttgcattaa ttcccagatt tttttattcg tttcaggact aaatgttgct tgagttaagt
     3481 tcttaaaata aagttcagga ccacactcgt caatcagcct catctgctct tccatttctg
     3541 gataaggatt ttctgaaaaa tcagcaaaca tgactttttt agttgtcggt tcaattgcta
     3601 ctaaagtctg acgcttaatt ggtttctcga gtaatttgca agctaaaatt ccactccaac
     3661 tatgtgcaca aagtatatat tcagaaattc ctaattcttc aagtacttca taaaccgcat
     3721 ctgcaagatt atctagattt tttccagctt ggtcatgaat cggactccta cctgtgttcg
     3781 gaaaatcaat tgtcaaataa ccaattgtag gaggaggttt ttcaagtata agtgaaaaat
     3841 tttcataact tggtagcaaa cctgctccgt ttagacaaac tagcactttc ttttgctttt
     3901 gataagtaac agagaggcta ccaatttctg tagatacttc aaacctcttc ataaagaaat
     3961 ccactcattc tatataatga attattaaaa atccttatct tttattttat cacgttccaa
     4021 ggattttctc aagttggagg aaggggacaa catctctact ttcccttcaa aaatccttcc
     4081 aaatcatgtt tatgttggta attaacggct gcggttttgt ctttctcaaa gacagtcttg
     4141 gtaaggtcaa tatgattaat agctacgatt gcgacggtgt agtaaatgat atcagccagt
     4201 tctctggcaa gttcctcgtt cgaatcctat cccttctttt cgaccagagc gcctattcaa
     4261 aacctcggct acttctccga cttcctccac taacttcata aagagacctt catcagtccg
     4321 agactgctgt taatgttcga ttaagtagtc ttggaattgc ctaaacgttc aatcttttat
     4381 agtatattga aactagaata gtacaccttt acttctaaaa cattgttaga aatcgatttg
     4441 actgtcctga tcgatttgtc ctgttcttgt ttcattttac tatatcttct attccacaca
     4501 aaaaagcgag acatccgtcc cgcccttctt atttttcgtc aataacgatt cttacttttt
     4561 tgtattcagt tgggacagag tagacaatcg ttcttatcgc agaaatagtg cgacccttac
     4621 gaccgattac acgacccaca tcgctttgat caagattcaa atgatattcc aaaaattctg
     4681 gtgtatcctc aatcttgata gttaaggcat ctggttgtga aattaagggt ttcacaatcg
     4741 caataatgag attttcaatc gtatccatct gtcaacctac tttaaactta ttttgaaaat
     4801 ttagaatcgt ggaatttttt caatacgcct tcttttgaaa ggatgttacg tactgtgtct
     4861 gaaggttgag ctccattagc caaccatgca agaacgcggt cttctttcaa agttacttgg
     4921 ttttcagcaa caagtgggtt gtaagttcca actgtttcga tgaaacgtcc gtcacgtggt
     4981 gaacgtgaat ctgctacgtt gatacggtag aaaggttttt tcttagaacc catacgagtc
     5041 aaacggattt taactgccat ttttaaagtc tcatttcttt aattttttat ttcggtgaaa
     5101 tagctgagct atttagcaca tgttctatta tagcagattt ctggcatgtg tcaagaaaaa
     5161 aacttgacag actataaatt tcttaaagta tttagaaatt tgttagaata ggaaataaat
     5221 gtttgaaaga tgctatccgt gaatactatt ttagaaaaat tttataaaga acaccaagtc
     5281 aaacccttca tatcgcctga acgagagttg gatacttggc ttctaagtcc caagcccgtt
     5341 cccaagcgaa atatggactt attagtagat gattcactag caggagatat cattttacta
     5401 tggagaatcc agtttggtac ttttaccact gaaacatgat tttaaaaact cccattcgaa
     5461 tatgattcaa aattcgctta gaatcaatgt tttcccgctt tttcaaaaaa taaattttca
     5521 tctaatttaa ataactttca attaagtgtg aattttgctt aaaatcatta aaaagcaccc
     5581 catggtgtga accatgaagt gttgtaaatg ctgtgttgta gctagttctt agcgtttaga
     5641 gaactggcta gctttacgag ctttcttaag atctggtttc caaaatactt tgagtacact
     5701 tatggtattg atgcccctaa acatctaaaa actctggtag aaaaagggta tgctcttgtc
     5761 gaaacttctt ttgactcact tgaccatcta aataatacca tgaaaaagaa tatcctgaag
     5821 aagaagggag ttactggact ttctaaaatg aaatcttgcg acctggatca agcccttcat
     5881 gagcattttt cagaagaaga attagctggt cactttcatg tccttctatg gactttttta
     5941 caatggcatt gctatcacac ccaataccta tctaagcgcc tggttcgtaa actttattgc
     6001 agctcttcct ctaaatttcc taattgttga accaattgcc cgttttatac taagttcttt
     6061 tcagaaacca tttactgggg aagaagttga agaagttgaa gattttcaag atgatgatga
     6121 aatcccaact attatctaag ccagttctgt aaactactaa tatttgaaat ccacttcttt
     6181 ttagggtaca atggtataaa tgaatttttg agaggatcag aatgaaaaaa ctagcaaccc
     6241 ttcttttact gtctactgta gccctagctg ggtgtagcag cgtccaacgc agtctgcgtg
     6301 gtgatgatta tgttgattcc agtcttgctg ctgaagaaag ttccaaagta gctgcccaat
     6361 ctgccaagga gttaaacgat gctttaacaa acgaaaacgc caatttccca caactatcta
     6421 aggaagttgc tgaagatgaa gccgaagtga ttctccacac aagccaaggt gatattcgca
     6481 ttaaactctt ccctaaactc gctcctctag cggttgaaaa tttcctcact cacgccaaag
     6541 aaggctacta taacggtatt accttccacc gtgtcatcga tggctttatg gtccaaactg
     6601 gagatccaaa aggggacggt acaggtggtc agtccatctg gcatgacaag gataagacta
     6661 aagacaaagg aactggtttc aagaacgaga ttactcctta tttgtataac atccgtggtg
     6721 ctcttgctat ggctaatact ggtcaaccaa acaccaatgg cagccagttc ttcatcaacc
     6781 aaagctctac agatacctct tctaaactcc ctacaagcaa gtatccacag aaaattatcg
     6841 aagcctacaa agaaggtgga aaccctagtc tagatggcaa acacccagtc tttggtcaag
     6901 tgattgacgg tatggatgtt gtggataaga ttgctaaggc cgaaaaagat gaaaaagaca
     6961 agccaactac tgctatcaca atcgacagca tcgaagtggt gaaagactac gattttaaat
     7021 cttaaaaacc aaaaaaatac agtatccaca ttcggtactg tatttctttt actctcattc
     7081 ttaagttaaa ttattaaaat cccatatttg gtctatccag ccttcataaa agtctggctc
     7141 gtggcagacc ataaggatag atcccctata ttctttgaga gcgcgtttga gctcatcctt
     7201 tgcatccaca tccaaatggt tggtcggctc gtccagcact aaaacgttgt tttcacgatt
     7261 catcaagaga cagaaacgaa ccttggcttg ctctccccct gataatactt gaatctggct
     7321 ttcaatatgt ttggttgtca aaccacaacg ggcaagggct gcacggactt ctgcttgatt
     7381 aagggcagga aaggcattcc agacagcttc aagaggagtt tggcgattac cgccttctac
     7441 ttcctgctca aaataaccaa gttctaaata atctccacgc tccacttccc cagcgattgg
     7501 cgagataatg cccaagagac tcttcaagag agttgttttt ccaataccat tagcaccaat
     7561 aatcgcaacc ttttgattgc gttcgaaggt aagatttaaa ggcttagtaa gaggaaggtc
     7621 gtaaccaatt tgcaagttct tggcttggaa gataaagcgc cctggtgtac gagctggttt
     7681 gaaatcaaag gatggttttg gtttctcact ttggagttcg ataatatcca tcttatccaa
     7741 tttcttttga cgagacatag ccatattacg agttgcaaca cgggctttat tacgagccac
     7801 aaagtccttg aggtctgcaa tctctttctg ctggcgttcg taggctgcct ctagctgaga
     7861 tttcttcata gcataaactt cttggaactg gtagtagtca ccagagtaac gcgtcagctg
     7921 ttgattttcc acatgataga caatattgat aacgtcattg aggaatggaa tatcgtgcga
     7981 aatgagaaca aaggcattct catagttttg gagatagcgc ttgagccaat caatatgctc
     8041 agcatccaag tagttggtcg gctcgtccaa cagcaagata tcaggctttt caaggagaag
     8101 ttttgccaaa agcaccttgg ttctttgccc acctgacaaa gaagttacat ccgtatccat
     8161 gccaaagtcc ataacaccaa gagcacgcgc tacttcgtca atcttagcat ccaaggtata
     8221 gaaatcacga ctctccagac ggtcttgaag ttctcctact tcttccatga gagcatcaac
     8281 atccgcaccg tcttcagcca tttccatata gaggtcattg atacgagctt cagctttgaa
     8341 aagctcatca aaagccgtac ggagaacatc acgcaccgac tgtctttcag caaggacaga
     8401 gtgctgatcc aagtaaccag ccgtcacata tttggaccac tcaacctttc cttcatctgg
     8461 cagcatttta ccagtcacga tactcataaa ggttgatttt ccttcaccat tggcaccgac
     8521 caggccgata tgttctccct tgaggagacg gaaggacaca tcttcaaaaa ttgcacggtc
     8581 accaaaaccg tgactcagat ttttaacttc taaaatactc attttaattc cttatcttgt
     8641 ttttatgtaa tcgtttataa aggagccaag ccagatagcc acccaaagtg ttggtccaca
     8701 aatcatcaat ctcaaagacg cgattgaaat caaagaaaaa gtccaagatt aattgcgtac
     8761 actcgattcc aagactcaca agaaaactaa aaagaaggac cttttttgtt ttccgcaaat
     8821 ttggaaatag ataaaggagt tggaaaatca gaggaaaaaa caagaagaca ttgaggatat
     8881 tttgtaaaaa aatccaacat aattgtccaa tgtcactcac ttcgcccagt ttccagagag
     8941 aattgaaagg agtcaaaaga aaaaccaggc gtccaagatg ctgaatatct ggagttccca
     9001 ctcccacggt agattgttct tgaggagtaa agcaaaaata gacaatgcaa atgctataga
     9061 aaatgactcc ccagaccaaa atatgattat aagtcttctt catcattaag gatttaccgc
     9121 tgcgactgcc ttctggcggt cacgtttcat tgtgttaaag agcaattgtc cacaagctgc
     9181 gtcaatatct gtaccatgct cttgacgaac cacacagttg cccccttttt tcttaagcgt
     9241 atcatagaaa gccaacacgc gctctttggg actacggcta tattggtcat gctcactaac
     9301 tgggttataa ggaatcaagt ttacataaga cgatttcttg atgttcttga gcaattcagc
     9361 caattccaag gcttgttcca caccgtcgtt gacttcatta agcatgatat attcaaaggt
     9421 tacacgacgg tttgttgtct caatgtagta ttcaatagca gcaaagagtt tt
//
LOCUS       DAVBCV010000071         8894 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBCV010000071 DAVBCV010000000
VERSION     DAVBCV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.071"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(135..1103)
                     /locus_tag="VQ516_001870"
     CDS             complement(135..1103)
                     /locus_tag="VQ516_001870"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2512262.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VQ516_001871"
     CDS             complement(1248..2063)
                     /locus_tag="VQ516_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2512263.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFTNTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAHKEQKVGKKQGLI
                     FLGLALRTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VQ516_001872"
     CDS             complement(2088..2585)
                     /locus_tag="VQ516_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2512264.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ516_001873"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ516_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2512265.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MSVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VQ516_001874"
     CDS             complement(4033..4548)
                     /locus_tag="VQ516_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2512266.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VQ516_001875"
     CDS             complement(4550..4993)
                     /locus_tag="VQ516_001875"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2512267.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VQ516_001876"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQ516_001876"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VQ516_001877"
     CDS             complement(5180..5647)
                     /locus_tag="VQ516_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2512268.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VQ516_001878"
     CDS             complement(5848..7134)
                     /locus_tag="VQ516_001878"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2512269.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VQ516_001879"
     CDS             complement(7365..7601)
                     /locus_tag="VQ516_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2512270.1"
                     /translation="MLQKIYEQMTNFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7867..8664
                     /locus_tag="VQ516_001880"
                     /pseudo
     CDS             7867..8664
                     /locus_tag="VQ516_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8699..>8894)
                     /locus_tag="VQ516_001881"
     CDS             complement(8699..>8894)
                     /locus_tag="VQ516_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512271.1"
                     /translation="RFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAYIKKNLK
                     KVLPSCNTFYEALFSCSCFN"
BASE COUNT         2851 a         2119 c         1430 g         2494 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcggta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc tcaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 tctttatggg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gtaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgtcatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatggcagg ttcatccaat
     2941 ttaacaccac cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagacataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtcttg gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttagggg aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaaaaccc gtagcacata ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccat cagccgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgctccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgactcc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgatta agacgctcat
     5281 ctgtcaagat atcgtacaaa cttccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgagctc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaaa ttttcacagt
     6121 atccaagccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgatac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttgatcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagtcatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
     7801 ctattagaac tataatattt atatatatgt attatggttc tgattttcta gtataaaaaa
     7861 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
     7921 gaggtcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
     7981 aagattgctc agaagttaat tgaaaagact tctatgaccg acattgatca tcagttgtct
     8041 atttcaactt caactgtcat tcgcaagatc aataattttc actttgagca tgatttttcg
     8101 cgtcttcctg agattatgtc ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc
     8161 gggagatgga gatgagcttt attgctcagg actttgataa actcaatatc ataactgttc
     8221 ttgagagcag aacacaagcc atcatccgaa atcccatgaa tacaaggcta tcaagcgcta
     8281 ctggaagctc attcaacaag atagtcagaa actgagtgat aaacgatttt atcgccctac
     8341 ttttcgtatg cacttgacta ataaagaaat tctagataaa cttttgagct attccgaaga
     8401 cttgaaacac cactataatc tctatcagct cttgcttttc cacctctgtt tcagactttg
     8461 tttaaaacct tcctcaaaga taaagagaaa atcgtcaacg cccttcaatt accttattcc
     8521 aacgccaaac tggaagccac caataatctc atcaaactta tcaagcacaa tgcctttggt
     8581 tttaggaact ttgaaaactt caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg
     8641 ctttcttcaa cccactacag ttgacaaaga accgaaaaat gtctcgcctc taatatagtc
     8701 aattgaaaca agaacaagag aaaagagcct cgtaaaaggt attgcaactt ggtaatacct
     8761 ttttgaggtt ctttttgata taagcccatg ttttctcaat aggattgtac tcaggtgagt
     8821 agggaggaag aggtaaaagt ttatgcccaa actcctcgca taaaagttct aacttaccca
     8881 ttctatggaa tctt
//
LOCUS       DAVBCV010000072         8830 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBCV010000072 DAVBCV010000000
VERSION     DAVBCV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.072"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            264..1322
                     /locus_tag="VQ516_001882"
     CDS             264..1322
                     /locus_tag="VQ516_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512272.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFDGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            1637..2611
                     /locus_tag="VQ516_001883"
     CDS             1637..2611
                     /locus_tag="VQ516_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512273.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSTK"
     gene            2991..3365
                     /locus_tag="VQ516_001884"
     CDS             2991..3365
                     /locus_tag="VQ516_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET2512274.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            3573..4475
                     /locus_tag="VQ516_001885"
     CDS             3573..4475
                     /locus_tag="VQ516_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512275.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            4531..4770
                     /locus_tag="VQ516_001886"
     CDS             4531..4770
                     /locus_tag="VQ516_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2512276.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINENPYPWDGFISATVR"
     gene            4767..5495
                     /locus_tag="VQ516_001887"
     CDS             4767..5495
                     /locus_tag="VQ516_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512277.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGKVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            6630..6869
                     /locus_tag="VQ516_001888"
     CDS             6630..6869
                     /locus_tag="VQ516_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512278.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            7419..>8830
                     /locus_tag="VQ516_001889"
     CDS             7419..>8830
                     /locus_tag="VQ516_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2512279.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKYEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKASEEEQAANLKYQQEL
                     VKYIRENDPTKKAEAKKAMDEAEKEYKKKQTEFAEVRAKVIPSAEELKKTRQKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIDES
                     DSEDYVKEGLRAPLQFELDVKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     QAGQYLAAAEEDLVAKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPEKPAEEPENPAPAPQPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPA
                     PQPGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLQYNGSWYYLNAN
                     GAMATGWLQYNDSWYYLNANG"
BASE COUNT         3046 a         1215 c         1616 g         2953 t
ORIGIN      
        1 taaatctaat aaattctatt attatagctc ttggtatgat attggcatta attttggtat
       61 tatcaagagt gtatagaaaa tatttttata gagacgatga tgactaaagt ttttatcaat
      121 aatttgggct ccttgtcaac tgtagtggat tgaagaaaag ctaacgtctg gggaagggtt
      181 taaagctttg ctttatcaaa tattgactta cagagttgct ctattgtatt gatgtattct
      241 tagtaaataa tatggtgttt tgaatgaaag atgatcagaa atatttatta gcgggacttt
      301 attcgcttct agttgcaata ttttattttc cattgattga gtccaagggg atttttgtaa
      361 gtatattgat ggcggtttta cttttatatt taatatactt cattgcaaca gtcattcata
      421 ttgtgataat taagtttata agaaaaaaat cttttaagta tttggttcta tatccattta
      481 cttatgatgg gagctggaga tttcaaccta taaacttact ctactttcca gaaatggtga
      541 gagatgtcat tccgattaat ttagttcaag aatattgcca aggacaacct tacggactgt
      601 taaaaaagat gctgaaaaga attcgattgt cacgagagat agctttactc ctggctacca
      661 ttatcgtcta tttttttacg catagaattc tacctctttc agtatttacg ttcatatttt
      721 cttacatatt gctatttgca caatcttatt tgggtggtaa taccgtttgg attggaaata
      781 gaagattaat aatagatgat gaatttgaaa aaatcttgct ttcaaaaagt tatataaaag
      841 aaatatcatc tgcacgatac tcggaatact tgacatgtga atacaaaaac cttacaccaa
      901 tcatattgtt agctattttt gaaaatttgc ttgactccta cttgcttcag aatcagagtg
      961 aggttgactt agatattttt tataaagttt tacctttgct ttataaagaa aaatatacta
     1021 tgggattcaa ttactttgta agtttaaatt atttactata taaggttgga tttttgggaa
     1081 tcatttacga taatgaagca ttaggagatt tgtcaaaaca atatttaaac agaaatattt
     1141 cggaattaca agatggttct ttcgatggcg gtatacaaga tgctgttgcc tctaaacaaa
     1201 tagtagttat aaatgaattt atagcttgtt taaattccag atgcatgcca tcccaatatg
     1261 atagattttt ttataaagat agaccttata ttttttccag aaagagtcct attaaggggt
     1321 gaaaatttgt tacatttgga ttaacaagtg tcctagggat tagaaataac tctagtatag
     1381 ctagatattt ttgagtaact agaaatatca ttaggctacc catatgattg ccagaaaaga
     1441 tggctgatga atcttattta tactgttgca gtaggcattg tctaggataa ataagctatc
     1501 ttgatggggg aaattggaat aattttagta gaaacattaa aggattatgg acaagtagcg
     1561 caggattagg tgatgcaatg tgctaagttg gcttggtgtt agcagtagta ttatttaaca
     1621 atggaaagta gactaaatga aaaataagcg ttattttttt gacacaattt taatgatttt
     1681 actgcttata agcacgatat tttgtgtcag cccagtattt ataaaattgg acatcttagg
     1741 aaccccctct catgccatct taacctttgt attggcgata ccgttattct atattttgtc
     1801 tcaatgctta catactttgc tattactagt ttcctcaatc ttttgtaaat taagaccaat
     1861 ttatttttat tttatatttg tgatcattat aggagctagg aaatactatc gaattctctt
     1921 tcaccaacta atgggattta gtccaggaat agcagtgttt tataaggaga gtcaaacaac
     1981 taagaatctg tttaaatttt actacttctt gtactttaca acactaattt catactattt
     2041 tttctttact tttgtgtatg acaaaccgtt gcttttgcct cttattccat tttctattat
     2101 catagcatta gtacaaaaac tttatagaat tgagaatcaa caattatttc tgttaaaatc
     2161 aaaggtacta acaatactgg aatctaagaa gaattgtgag tttaatcttc aggattatca
     2221 tgaaatttgg aaattgcaat caaaatctga gttgccatgc gtggctttat cttatatcag
     2281 tttgatcaag ccatatcttt cagagagcgt gcgtgaacaa attgatttat tagaagttaa
     2341 gcgatttaaa aaaataaatc atccaatatc actttatggt atgttggatg ttataaaatt
     2401 aaatctatat ttacggcatt ataatgaaaa aaataaatat gaatcaatgt taaagaaaat
     2461 tttagaggta agaccagact ttgttttaat cgagcagaat attgatgata gtttaaatag
     2521 ctcgcaacca ttatctttat ctttagccat ttcagaaatc caacttttgc ttgaagtgta
     2581 tatggggatc aaacatgttt caaccaagtg ataaaaccag aataatattt gccattatag
     2641 gggcaagttg catattagtc agtatgtatc ttattccact tattaaaact gagaataaat
     2701 tttgggaaat acttttattt atattaggtg tattgctaat tggaattgcg ttactaagtc
     2761 aaaaaaaata aattttgaaa tagtattagt gagttcgcaa tcatttattg ctagtggttt
     2821 agaactgtta agtttcaaac aataattgat tttaaacctt aggtaaccta taaaaacaaa
     2881 taaggtagag aaaggatatc ttatctaggt agctaagtga taaaaacaca gtcgtctatc
     2941 acttgttagg aaactatcct aactatgctt aataaaaaag gagaaaataa atggaactcg
     3001 tattaccaaa taattatgtt gttcttgagc aagaagagat gatgtatctt gatgggggat
     3061 tttctattcc gagatggcct gttgcaacag ccattaatat agcttttaat ggtgttttag
     3121 gtggaggagc aatcagtcta gttagaaatt atattcgtaa ttatggtttg cggcgagtta
     3181 caagcgcaat tgctggagca gctgcaagat atgttggggt acgagttgca aatagagtgg
     3241 caggatttgc actgtctgct attaatggat ttgcagcttg gatgtcaatt ggcgatgcta
     3301 ttacaacaat ctgggccaac aatgatgtaa ataggagaga cccaaattta aacgccttgt
     3361 ggtaactaga tatgggtatt aaaatggaaa aaatttttgt tattattttt tttgtatgtc
     3421 tatttatatc atccataact tttttagcct atgattttgt tagcgaagaa ataaaaaagt
     3481 tgattatttg gattaatgtc gtttttttga ttttaatcat agcaatgatg atttatccaa
     3541 aattaagaaa atgataagga cactgtaaaa ttatgatacg taaactaatt gttttcttac
     3601 taatgctccc tctttttgtc ttgtggctag agttgcatgt cctagtgaat aacttactat
     3661 tgaatctaga atctcccctt gattttgtga ttagtatgag tcttgctttc tgtagtttga
     3721 ttttatcaaa aatagtttta gacatactat acgcattgaa agatttatac aagaaagaag
     3781 gtcttattaa gatttttccc tttattttta taagaaggaa aaaagtgaat gtgagattct
     3841 cgccctattt ttcattccat cgtaaaagtt tgtcgcctga tgatttaaga tctaggatta
     3901 tatggagttt tattcttgaa attgctatta tcctagtatt tattttaaaa attccttttg
     3961 caatcatcat gcttactaca atatttttct ggactataat ggatatcaat catctcgttt
     4021 ttaataagac ggaatttctt tttaatcaaa acaaatggca aaaagaagat tcatttgaaa
     4081 gtaatttgca taagatattg atggacaaaa tacaaaaatc agaattaagc tattctgact
     4141 taatgtccct tcttctctat gatgcgatga accaatccac ctttttaacg gatagtgaac
     4201 tttttgagga tattttgaaa aaaatcgaag attctcataa cacccttctg tgtacaggtc
     4261 ttgtcgagtt actgctgtat gagatttcta ttagtaacaa taacaactgg caagaaaagg
     4321 ttgataaaat tagaatacag ctcatcagaa tcaatcaatt agattttttc tattatacaa
     4381 gttggttaag gcaaaatttt gatttctgta tgaatagaga gtatcataaa atgaaatctc
     4441 ggaaattatt actgagtaat aaaaagatag tttaagttcg tcctatttgt aaaacaaatg
     4501 aaacaatttc aactaaggag gagaaaacaa atggaacttg tattaccaaa taattatgtt
     4561 gtgattgatg aagaagtaat gatgtatctt gatgggggag cttatttaag caagcgtgct
     4621 tgtcaaggaa tttgcgcagc agcaggtgta ttggctacag cagcagggaa aatagcttac
     4681 tgtattggat atggtgttct taatagaggt tgtgatatta acgagaaccc ttatccttgg
     4741 gatggattca tatctgcaac agtaagatga gtaatgtaga taagattcga aaaatacaca
     4801 tcatcgtgtg ttgggtgtat atatttttat cattcagggc gattataaat gatacggagt
     4861 attttttgct tattttttta gcttttatct actcaatagt atcactacct ctatattctg
     4921 tgaaaaataa aatagtatct atctgtctag ttataaattc gattctgtta atgagtttcc
     4981 ccattttaat caataagttt ttcccagaaa gttttttgac ttataccgta ttaataagtg
     5041 tttttatcct agagttagca atctttcatt taattaggaa agattttgat attaaattga
     5101 ctaacgaata taagaaaatt agtcagttta gaagcaaggt gtctcaatct ccttggataa
     5161 aatatttaga gatttctagt tttatattaa ctatatttcc atctattctt tatggtaaag
     5221 ttgataatca tgtactgact cttatctttt tgataaaaat ttgtgtagat actacgataa
     5281 aatttttatt catcagatta tttgacacaa gtactttaat gaagaggaga atattttttc
     5341 ttttcgcatt ggatgttata gcttacttat ttttaggata tcttttagtg attcaaaaag
     5401 caggctattt gttttcggtt ttacttcttt tttctaattt ttcagttcca tttatcaaag
     5461 aaaaggaata cgaattattt aaaaatagca agtaatagaa atatggatta aatcttagaa
     5521 cggcttaaaa tcagatatga taaagggaaa tagtcttatc taggtaacta agtgctacaa
     5581 acacaatcga ggatcacttg ttaggattac attttaatca tgcttaataa gaatggagaa
     5641 ttcctatggg gattgtatta ctctgaagtt tcgaatacct tagttctcgt gattttgagc
     5701 tattctagct ttagaatcct tcagaacgta aaattttaag cagtcaaaga cttagaatag
     5761 cctgaaaaca tttattttat agcaattcta ggcttagaat tgctcatata tatgtatttg
     5821 agctgctcga tagttaggac agctctattc tgagaaatct gagctatcaa tgcttcagaa
     5881 tagtatttcg ccaatggttt ttattgaaga ggtgattgtg ttatgatagt ctaaaataaa
     5941 attgatttaa tagagtaaat ggagaatcgt tacatgaaac atttttttgc aggaattggt
     6001 gagataaaac ttgttggcta cttattgtat atatgtgtag gactagcccc tctttttcat
     6061 atatatgtca taggttcgga actgagtttt gtgaaaatag tgttatctat tttgggagtt
     6121 atatttgtaa gtatgttaac tattgctcga atttatcgga cttttctata aagaatagtt
     6181 tggacttaaa ctgtatctaa aaacagaggt tggtcgatta tcagcctctt tcttgtcgtt
     6241 gaaaagagtg agatatattg acttttgaaa aaaagatgtc ataattgagt tgggataaac
     6301 ctaaaagtaa tatagtagat tgaatctgga atagtccgtt atgactgtta aaatatttct
     6361 agaaattaat ttgactttcc taatcgattt gttcacatct tgtttcaatc tactatactt
     6421 tctcgttaaa ccgttcggca attcatggtg acatggacac atagtagtaa gcacactatg
     6481 tggcttggca gagctaaaaa ctgttccctt gcgataagcc taataagcac aacataggga
     6541 gttagagaag ccgactctaa tcaaccactt tgggcaatag tgagaactgc cccgtgcttt
     6601 tttattttga gaaaatatgg agtttgtcgt tgaaattact tgattgtatt ttggactatc
     6661 aagaaagatt caatggaaaa acatgtcaag tatcaacgaa ttataagtat ttagagattt
     6721 tcaaagtgaa tttttgtttg actgatttac atcatttatt tgacttatac aaaatcacac
     6781 gagattatgc tagtcaaaca aaacctgcta ttcaagatgg tgtttttatt ttagaagatt
     6841 ttagaaatat cctatgtaca atgatgtaat cgaaaggata tctttatgtg agtttatcgg
     6901 cgatatcttc tatatctaag ctagcaagtt gttgcatcgt agctaaggat ttatctaaaa
     6961 atactatgaa attggacgtc atattttttg aggataaaaa taaaagatcc gcagttttag
     7021 gtttacgaag agacaaaagc ggagtattta aaccagttac tctacatttt acaagcgcta
     7081 agaaatatgc taaagttcgt aaaacagatg tgaaagaaat gaaatggcta taaagattgt
     7141 ccgcaggctt aagcttgcgt tttttgttgt ttaacgaaat taaaaaacaa gctctctcat
     7201 cggaaatgtt tttataggaa tgaaggaaga tgatgctatg tttaaaaaga gaatcgaaaa
     7261 gggcaaaagt agtgtcttta tctttttgga gtagaagctt atgatataga aatttgtaat
     7321 aaaaatgtaa tataaaacat ttgacaaata tttacggggg gagtatactt aatataagta
     7381 tagtctaaaa atgactatca gaaaagaggt aaatttagat gaataagaaa aaaatgattt
     7441 taacaagtct agccagcgtc gctatcttag gggctggttt tgttacgtct caacctactt
     7501 ttgtaagagc agaagaagct cccgtagcta gtcagtctaa agctgagaaa gactatgatg
     7561 cagcagtgaa aaaatatgaa gctgctaaga aggaatatga ggacggaaaa gctgctcaga
     7621 aaaagtatga agatgatcag aagaaaactg aggagaaagc ggaagaagaa agaaaagctt
     7681 ctgaagaaga acaagctgca aatctgaaat atcaacaaga gttggttaaa tatatacgtg
     7741 aaaatgatcc aacaaaaaaa gctgaagcta agaaagcaat ggatgaggct gagaaagagt
     7801 ataagaaaaa acaaacggaa tttgctgaag ttcgtgcaaa ggtaattcct agcgcggaag
     7861 aattaaaaaa gactagacaa aaagcagaag aggctaaagc aaaagaagca gaacttacta
     7921 aaaaagtaga agaagctgag aaaaaagtta ctgaagccaa acaaaaagtg gatgcagaac
     7981 atgctgaaga agtcgctcct caagctaaaa tcgctgaatt ggaacatgaa gttcagaaac
     8041 tagaaaaagc tctcaaagag attgatgagt ctgattcaga agattatgtt aaagaaggtc
     8101 tccgtgctcc tcttcaattt gaattggatg ttaagcaagc taaactatca aaacttgaag
     8161 agttgagtga taagattgat gagttagacg ctgaaattgc aaaacttgaa aaagatgtag
     8221 aagatttcaa aaactcagac ggtgagcaag ctggacaata ccttgctgca gctgaagaag
     8281 acttagttgc taaaaaagct gaattagaaa aaactgaagc tgaccttaag aaagcagtta
     8341 atgagccaga aaaaccagct gaagaaactc cagctccagc accaaaacca gagcaaccag
     8401 ctgaacaacc aaaaccagcg ccggctcctc aaccagaaaa accagctgaa gagcctgaga
     8461 atccagctcc agcaccacaa ccagagaagt cagcagatca acaagctgaa gaagactatg
     8521 ctcgtagatc agaagaagaa tataatcgct tgacccaaca gcaaccgcca aaagcagaaa
     8581 aaccagctcc tgcaccacaa ccaggttgga aacaagaaaa cggtatgtgg tacttctaca
     8641 atactgatgg ttcaatggct acaggttggc tacaaaacaa cggttcatgg tactacctca
     8701 acgctaatgg tgctatggct acaggttggc tccaatacaa cggttcatgg tactacctca
     8761 acgctaatgg tgctatggct acaggttggc tccaatacaa cgattcatgg tattacctaa
     8821 acgctaacgg
//
LOCUS       DAVBCV010000073         8519 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBCV010000073 DAVBCV010000000
VERSION     DAVBCV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.073"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            218..1408
                     /locus_tag="VQ516_001890"
     CDS             218..1408
                     /locus_tag="VQ516_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2512280.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(1729..2610)
                     /locus_tag="VQ516_001891"
     CDS             complement(1729..2610)
                     /locus_tag="VQ516_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2512281.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(2751..4079)
                     /gene="vncS"
                     /locus_tag="VQ516_001892"
     CDS             complement(2751..4079)
                     /gene="vncS"
                     /locus_tag="VQ516_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2512282.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(4076..4732)
                     /gene="vncR"
                     /locus_tag="VQ516_001893"
     CDS             complement(4076..4732)
                     /gene="vncR"
                     /locus_tag="VQ516_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2512283.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(4781..4864)
                     /gene="pep27"
                     /locus_tag="VQ516_001894"
     CDS             complement(4781..4864)
                     /gene="pep27"
                     /locus_tag="VQ516_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2512284.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(5044..6423)
                     /gene="vex3"
                     /locus_tag="VQ516_001895"
     CDS             complement(5044..6423)
                     /gene="vex3"
                     /locus_tag="VQ516_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2512285.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(6475..7122)
                     /gene="vex2"
                     /locus_tag="VQ516_001896"
     CDS             complement(6475..7122)
                     /gene="vex2"
                     /locus_tag="VQ516_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2512286.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(7135..8412)
                     /locus_tag="VQ516_001897"
     CDS             complement(7135..8412)
                     /locus_tag="VQ516_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2512287.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITSYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         2415 a         1862 c         1614 g         2628 t
ORIGIN      
        1 acaacctcaa aacggtgttt tgagcagcct gcggctagct tcctagtttg ctctttgatt
       61 ttcattaagt ataaacagcc actctccagt tcaattggag agttttttga tgagagtaga
      121 tatttataga cctcaatttg acaccataca ttttctatga taaaatgtaa caaattcatt
      181 tacaacaaat cgaaatctaa atgaaatgga attttctatg aaatcagtca aaggactact
      241 ctttatcata gctagtttta tcttgactct tttgacttgg atgaacactt ctccccaatt
      301 catgattcca ggactagctt taacaagcct atctctgact tttatcctag ccactcgtct
      361 cccactacta gaaagctggt ttcacggttt ggagaaggtc tacaccgtcc acaaattcac
      421 agcctttctc tccatcatcc tactaatctt tcataacttt agtatgggcg gtttgtgggg
      481 ctctcgctta gctgctcagt ttggtaatct tgccatctat atctttgcca gcatcatcct
      541 tgtcgcctat ttaggcaaat acatccaata cgaagcttgg cgatggattc accgcctggt
      601 ttacctagcc tatattttag gactctttca catctacatg ataatgggca atcgtctcct
      661 tacatttaat cttctaagtt ttcttgttgg tagctatgcc cttttaggct tactagctgg
      721 tttttatatc atttttctat atcaaaagat ttccttcccc tatctaggaa aaattaccca
      781 tctcaaacgc ttaaatcacg atactagaga aattcaaatc catcttagca gacctttcaa
      841 ctatcaatca ggacaatttg cctttctaaa gattttccaa gaaggctttg aaagtgctcc
      901 gcatcccttt tctatctcag gaggtcatgg tcaaactctt tactttactg ttaaaaattc
      961 aggcgaccat accaataata tctatgataa tcttcaagcc ggcagcaaag taaccctaga
     1021 cagagcttac ggacacatga tcatagaaga aggacgagaa aatcaggttt ggattgctgg
     1081 aggtattggg atcaccccct tcatctctta catccgtgaa catcctattt tagataaaca
     1141 ggttcacttc tactatagct tccgtggaga tgaaaatgca gtctacctag atttactccg
     1201 taactatgct cagaaaaatc ctaattttga actccatcta atcgacagta cgaaagacgg
     1261 ctatcttaat tttgaacaaa aagaagtgcc cgaacatgca accgtctata tgtgtggtcc
     1321 tatttctatg atgaaggcac ttgccaaaca gattaagaaa caaaatccaa aaacagagct
     1381 tatttacgaa ggattcaagt tcaaataagt aatcatactc tcctgggatt tcccaggaga
     1441 gttttttata gccaaatgaa ataaaaccgg aacgatggaa ttttggcatt caaatcaatt
     1501 gataagaata ttttatagtg gagtgaaata ggatacgaac aaattgatta ggaaaatcaa
     1561 atgaatttat agaaatcttt tagcagttat gttgtcctat tctagtttca aaacgctata
     1621 gaagcagcat tgtgctagtc tagattcagt ttactatact aaaacgagta gcttgaaatc
     1681 aaaaaaccca ccctcacagg caggttttat ctgtattatt cagctagatt atgctttacc
     1741 ttctgaaccg aatacgtcga tacgttcttc aaccgatgct tggatagctt ttacaccgtc
     1801 agccaagaat ttacgtgggt cgaagagttt tttcttgtcg tattctgctt cgtttgcttc
     1861 gtagtcacga gcaaatttac gagttgcgtt agcgaatgcg atttggcatt ctgtgttaac
     1921 gttaactttg gcaacaccaa gtttgatagc tgcttggatt tgctcatcag gaatacctga
     1981 tccaccgtgc aatacgattg ggaatcctgg aagagcttct gtcaattttt gcaagtggtc
     2041 aaggtcaaga ccttcccagt ttactgggta agggccgtgg atgttaccga taccagctgc
     2101 caagaagtcg ataccagttt caaccattgc tttagcgtct tcgattggag ccaattcacc
     2161 tttaccgatg attccgtctt cttcaccacc gatagtacca acttcagctt ctactgagat
     2221 acctttagcg tgtgcttttt caacaacttc tttagccaat ttaaggtttt cttcaactgg
     2281 aaggtgtgaa ccgtcaaaca tgattgaagt ataaccaact tcgatacact caagtgcatc
     2341 ttcgtagtga ccgtggtcaa ggtggatagc tactggtaca gtgataccca ttgattcaac
     2401 aaggttagcg atcaagttgc gagcaacttt gtaaccaccc atgtatttag cagcacccat
     2461 tgaagtttgg atcaaaactg gagctttttt agcttctgct gcgcgcaaga tagcttgagt
     2521 ccactcaagg ttgtttgtgt taaatccacc aactgcataa ccgttgtcac gggctgcttg
     2581 gacaaatttt tctgctgaaa cgattgccat tttatcaggc ctcctgtata tttttatggg
     2641 tcatcccatt tacattgttc attttatcac tttttgccaa aaaaatctag tttttcccgc
     2701 agtttcgatt gattttcttc taactccatc tatgtaaacc ctttctctcc ctagtcttgg
     2761 acgacttttg gaaaatctat aaagaaggtt aaacgattct cctccatctc gaaacgataa
     2821 gctaattttt catgttctaa tagactctta accacaaaga gccccatacc agaccccttg
     2881 accttgcgac tggcattgtc agaaaaagac tgggctagtt tttcttgttc ctctgagcta
     2941 cagctatttt cgataaaaag ttctccttct ctttctccaa ttcgaactaa gccacctgga
     3001 acagagtgct taatggcatt gctgatgaga ttagaaagaa tcaacttcat aactgatggg
     3061 tttagataag cctgctgatg ggtcaaacta ttgtctatct ggagctctct ttccttggct
     3121 agcaaggcat aatctttgac cagattttgc gtcatctgga ggaggtcaat tgtttcccta
     3181 tcatctcgca attcctgcac agaagagagg gaaagtatct gcagaacatg gtgattgagt
     3241 tcatccacaa tccccaaggc aactcccaga tactggtctc tatccttata acgaccgata
     3301 ttctctctca tattttcgat taggattttc aaactagcca gcggtgtttt caattcatga
     3361 gaagctcctc gtaggaattc gaccttcatc ttctccagct ggagaatggc ttcattcttt
     3421 tcatgcaagt ccgcaataac agtcaagaga tgctggtaga ggctattgat ttgttccttg
     3481 agattaccta tctcatcctt agaatccacg cgcaatcgca cttgggaatc caggtccatc
     3541 atccgacggg tcacccgctt gatttccaag atcggtgcaa caatagtccg agcgtagatg
     3601 taggccacca agagggaaat cagaaaggag gccagcaagg tatagggaag aaactggaga
     3661 ctgatttgct ccgcttcctt ttgtaaatcc atggaagcta gaaactggag aatcatagta
     3721 gcaccgtctt gcgttttcac ctcgcgctcc tcaataaaga gagaggttgt ctggcggtct
     3781 gtgtccagag gaagactgtc cttgacttct aacttgtcct cggtcatctc acctttgacg
     3841 gtccccttga tatcactagt ctgggaatac aagtctaaca cttgctcgat actctgccta
     3901 tctttccctt ctagggactg ggcaatggct gttgcctttt gaccaatggt ttcctgacga
     3961 tgactcagat aagtcgaagg aaaaagaaaa taaatagcta aatgaaggca gataaccaga
     4021 acactaaata tcgagaaggt atagataaat atctttgcaa ataaacctgt tcgtttcatt
     4081 ttcgctccaa tttataacca acattgcgca cagtgaggat acaatccaag tctagctttt
     4141 tccgcaattc cttgatataa acatcaataa cacggtcaaa gggaacctca tctgtcgctt
     4201 tccagacggc atcgataatc tgagatcgag tcaaggcccg gccttcattt ttcactagat
     4261 agtccagaat ttccaactct ttggcattga taggcacttc ttgacctgcg atgcttgcac
     4321 tgtagctttc aaagtccacc ttggtatcct tgtaagaaaa gattcgtcct gtatcgtagt
     4381 agcgcttgaa aatcgcgtcc accctcactt ttaaaaggga gagggagaaa ggtttttcca
     4441 gatagccatc tgccaaagag gcaaaggcac tcatcttgta ttcctcatct tgaaaagctg
     4501 tcaacatcaa gacaggaacc tgactggttt tacgaatctc agctaggact tctaagccgt
     4561 tgagcttggg catctggata tccagtaaaa ccagggccac ctcatagcta gaaaattgct
     4621 ccagagcttc ctgaccgtcc gctgcctcaa tagtttcata gccacaatcc gtcaaataat
     4681 cactgacccc ctcacggatc atctcttcat cttctacaat taaaattttc atactttaac
     4741 tgctctctat tttttatttt tcttagaata aatacctact ctattttcta ttatagtctc
     4801 ttgctggcct tttgtctgca agcaactgac cactagataa aacgttgtga aattcctttc
     4861 tcataaattc cataacttta gtatattata tttaagcact aaagtacaaa gaaagcaact
     4921 gaaagcaatg attttcacca ctgctttcgg atttattttg aattgttaaa tagccattcc
     4981 tatccactat tcttgaatag aaacacaaga tgcaatcttt attctagact cattttttca
     5041 aatttattca ccatccagca agagctcttt tggttgtttt ctaaggagat tgcttgaagc
     5101 aagcgccata acgagaacca ctagaaccaa ggcaaggaca aaaatgatga taaagtctga
     5161 tgtctgaatg gaaatgtcta ggctcgacaa ggtcttgcta aagccatcta cttctgcacc
     5221 accaccaagg ttagaggctt gagccgcctt actagcctgt ttggcaacac ctgaagtcac
     5281 attggcaagg acagtgtttc caattgcacg ggcagtgtaa ttagctagga agtaagcaga
     5341 aactagagca gggatagcaa tcaagataga ttcggtgatg aattgaccca agatacttgc
     5401 ctgcttgagg ccgatagaga ggagaattcc cacttccttg cgacgggcgt tgatccaaag
     5461 gctgagcaag agggcaagga ggagaactga gaagctcaag ctaccccaga agaggaggtt
     5521 ggccatcttg tacataccag agatagattg ctcaagagct gggtagttag aggagctctt
     5581 gacgagtgtg tagctcttcc agttgatacc actgatgcca ttcaactctt tcataacatc
     5641 atccaagttc ttgtctgctg ttacaaagaa ggttgcgtcc ccataaatgg ctgtgtcttc
     5701 tgtgtatcca taaagttttg cagcagtgtg aatgtctgta atagctgtgt tttcgtaaag
     5761 ttcttgtgag taggttactg ctgacttatt atgaccatca aagagtccct tgattgtcac
     5821 ttcaactgtt tccttggctc ctttttcatt atctgcatcg tagatattag agtccagttt
     5881 aaccttgtcc cctactttcc agccgtgttt ggctgccaag tccttgtgca agaggatttt
     5941 atccttgtcg tcgttggtta agtgctctcc ttcgactagt ttataagaac cagagacaaa
     6001 cttgtcttct ttagaggagt cattgacacc tgtaatcatc aagctacttc caaaacgctt
     6061 ggcacgatca gcagtgagat tcttcttggt ttctggcgtt tcaatcaggt catatccagt
     6121 caaatctccg atagcgttga tacgtttgac ataagactcg atggccttgt tttcggtgat
     6181 ttttttgatg tcttcaccct tgatattccc agcaccacga ggcgttcctt ggttgacgcg
     6241 acgattgatt tgcatggaga agctattagt gatattttta aaggtctcct gagaagcctt
     6301 ggcagtagct cccttgattg acaagccgac caaactcaag ctcgccatga ggagaataat
     6361 caggaagatg acaatcgatt tgaaaaactt ccttgtaaca taggcaaatg cgttgtgtaa
     6421 catagattcc ctttctagat tttgttttaa tcattctatc aaaataagct caaattattt
     6481 actagtattg cgcgtttcag tcagtttctt atcctttaat tcaagtgtaa tatctgacgc
     6541 ttgtgccact tctttactgt gagttacgac aatcacacat ttacctgttt tctgggcaag
     6601 tgatttgagt agttcgacaa tatctccagc agttttagga tccagatttc ctgttggctc
     6661 atcagctaga ataactggag cttctgagac caaactgcga gcaatggcaa cacgttgctg
     6721 ttgaccacct gataactgga gaacattccg cttgatctgg ctttcatcca aaccaagctc
     6781 aagaagtgta tccttgcttg cctttttgtt gaccaagcgg atattttcca gcggagaaag
     6841 ataatctatc aagttataat tttgaaagac cagggaaata tggtgcatgc gatggtaaga
     6901 atagcccttc ttacgaatat cctctccttg aaaaaggata gaaccttcaa caggactatc
     6961 tagaccagca agtagggaca agagtgtgga ttttcctgct cctgactccc caataatact
     7021 gtaaaatttt ccaggttcaa aattataatc gatctgatat aggactgctt cagcagtatt
     7081 cttataacgg taggtaacat cttgtaattg taataaagtc atgatttctc cttcttaact
     7141 aatagatgat aaaatttctt tcggtgattt tctaaataag aataggaaac aaagggctac
     7201 agataagcaa ctaagcagaa ctagaaaaac ataggattct gcaaaagata agatgctagt
     7261 tgataaactg cttgctttgg ctagtgtatc ttgtaagctt gcctgatctc cacttgctag
     7321 tagagtttgg agtagataac ttgtgattcc gtttcctgca acaaatgctg gaagcaaagc
     7381 tccaagagat accaaaacta cctctaaaca gaattgtagg aagatcgagc tcttgccttt
     7441 tccaagtgca agtaaaatcc ccacttcata gacccgttct ctcaaccaga gagacaaaac
     7501 cagaattaag gctccagctc ctgctatcaa catcccataa aggaagatgg tcaagaaggt
     7561 ttggaaagtt gcaactgagt ctttgatttg ttcaaaagcc ttgttttcct tttcgacttg
     7621 gtagccttga ttttccaagg ccaagttttc tacctgcttc atgagtccgt ccatttcctt
     7681 aggattttct acatagaagc gtgctgcact gacttgagct tcactattgc ccaaaagggt
     7741 ttggctactt tcatagtctg taaagacttg attttcactg aagtcagaag acaagcctgt
     7801 gaatttctct tgttttttac cagaaaagat gccgataatc tcaaactcta ctgtttgtcc
     7861 ttttccagat tcagactgac cagcatccaa gccaatcttg tcatgaagtg aaagaccgtt
     7921 cttcttagcc aattcttcgt ggataaggat tttcttggaa tccccttttt gaaggtgtcg
     7981 cccttctttt agattgaaag ccgaactggt aaaggttaca tccttggatg aatcctcaag
     8041 agccgttaag ctaaccaagt tattgtctgc agctgataaa tcatcacgct ccacgctctg
     8101 ctcaccagtc actgcttcct tgtcttttag ttttgcgacc gtctcaagtt caggagagac
     8161 attttccagc cccttaatct tgcttacaga tgctaggtct gacaacttga atgtctgacc
     8221 attctctatc ttcttaatag aaaaagatgt attgagtgat ttataaagat tgctttctac
     8281 tgttttgttg gacttcatca gagtcaaaca ggctgaaatt ccggccaata agaccaataa
     8341 aatcagaaat aaaataaaac ttctcagtcg ctttctgctg acataagccc aagatctttg
     8401 gattggattc atttgtcacc tccatatttg taagactatt ataaaaccca aatatgaaat
     8461 gtttatgaaa tacgaaaaaa aatatcgagt aggggataat ctctagcccc tctcacacc
//
LOCUS       DAVBCV010000074         8109 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBCV010000074 DAVBCV010000000
VERSION     DAVBCV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.074"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            120..497
                     /locus_tag="VQ516_001898"
     CDS             120..497
                     /locus_tag="VQ516_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2512288.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            732..2123
                     /locus_tag="VQ516_001899"
     CDS             732..2123
                     /locus_tag="VQ516_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2512289.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            2237..3079
                     /locus_tag="VQ516_001900"
     CDS             2237..3079
                     /locus_tag="VQ516_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2512290.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            3352..4113
                     /locus_tag="VQ516_001901"
     CDS             3352..4113
                     /locus_tag="VQ516_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2512291.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            4483..5400
                     /gene="glyQ"
                     /locus_tag="VQ516_001902"
     CDS             4483..5400
                     /gene="glyQ"
                     /locus_tag="VQ516_001902"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2512292.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            5660..7696
                     /locus_tag="VQ516_001903"
     CDS             5660..7696
                     /locus_tag="VQ516_001903"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2512293.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            7738..7995
                     /locus_tag="VQ516_001904"
     CDS             7738..7995
                     /locus_tag="VQ516_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2512294.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         2555 a         1431 c         1794 g         2329 t
ORIGIN      
        1 gctttctttt ccgaataaat agatagaagc atagaatcta gcaaacctag atttaaaaat
       61 gtgctataat agaaggagga aaaggaggat tctcagacat ccaggtatca gcccaactaa
      121 tgatttgtca atttatccgc gatatgctgg acttgccagc aaaaaatgtg acgattttgg
      181 agggaagtaa cattcacgtc ttgccttcca tgccctactc agcgcaagat ttctatacta
      241 gtatagacgt cttggcggag ttagataatg gaatccaagt tatcatcgaa attcaggttc
      301 atcatcagaa ttttttcatc aatcgcctat gggcttatct gtgcagtcag gttaatcaaa
      361 acctagaaaa aattcgccaa cgtgaaggtg atacccacca gagctacaaa caaatcgcac
      421 tagtatacgc tatcgcaatt gtcgatagta attacttctc agatgaccta gcttttcata
      481 gttttatagt aaaatgaaat gagaacagga caaatcgatc aggacagtca aatcgatttc
      541 taacaatgtt ttagaagtat aggtctacta ttctagcttc aatctactag aaattccata
      601 gatagaaaac tacataatct ctacagatac ggatgttgga gttgatgtaa gatgctttgg
      661 cttgctagag gaattgtgga ttgccaaatt gtatcattga aattattgct caaatttgtt
      721 atgatataaa tatgaataaa agtagactag gacgtggcag acacgggaaa acgagacatg
      781 tattattggc tttgattggt attttagcaa tttctatttg cctattaggc ggatttattg
      841 cttttaagat ctaccagcaa aaaagttttg agcaaaagat tgaatcgctc aaaaaagaga
      901 aagatgatca attgagtgag ggaaatcaga aggagcattt tcgtcagggg caagccgaag
      961 tgattgccta ttatcctctc caaggggaga aagtgatttc ctctgttagg gagctgataa
     1021 atcaagatgt taaggacaag ctagaaagta aggacaatct tgttttctac tatacagagc
     1081 aagaagagtc aggtttaaag ggagtcgtta atcgtaatgt gaccaaacaa atctatgatt
     1141 tagttgcttt taagattgaa gagactgaaa agaccagtct aggaaaggtt cacttaacag
     1201 aagatgggca accttttaca cttgaccaac tgttttcaga tgctagtaag gctaaggaac
     1261 agctgataaa agagttgacc tccttcatag aggataaaaa aatagatcaa gaccagagtg
     1321 agcagattgt aaaaaacttc tctgaccaag acttgtctgc atggaatttt gattacaagg
     1381 atagtcagat tatcctttat ccaagtcctg tggttgaaaa tttagaagag atagccttgc
     1441 cagtatctgc tttctttgat gttatccaat cttcgtactt actcgaaaaa gatgcggcct
     1501 tgtaccaatc ttactttgat aagaaacatc aaaaagttgt cgctctaacc tttgatgatg
     1561 gtccaaatcc agcaacgacc ccgcaggtat tagagaccct agctaaatat gatattaaag
     1621 cgactttctt tgtgcttggg aaaaatgttt ctgggaatga ggacttggtg aagaggataa
     1681 aatctgaagg tcatgttgtt ggaaaccata gttggagcca tccgattctc tcgcaactct
     1741 ctcttgatga agctaaaaag cagattactg atactgagga tgtgctaact aaagtgctgg
     1801 gttctagttc taaactcatg cgtccacctt atggtgctat tacagatgat attcgcaata
     1861 gcttggattt gagctttatc atgtgggatg tggatagtct ggactggaag agtaaaaatg
     1921 aagcatctat tttgacagaa attcagcatc aagtagctaa cggctctatc gttttgatgc
     1981 atgatattca cagtccgaca gtcaatgcct tgccaagggt cattgagtat ttgaaaaatc
     2041 aaggttatac ctttgtgacc ataccagaga tgctcaatac tcgcctaaaa gctcatgagc
     2101 tgtactatag tcgtgatgaa taagcaagaa aaaataggtc tgttagatat ttgacagact
     2161 tattttttac agaatatagt actacttaaa aaatgtttta tgctataatt gatgaataaa
     2221 atagaaggag aagcatatga atacctatca attaaataat ggagtagaaa ttccagtatt
     2281 gggatttgga acttttaagg ctaaggatgg agaagaagcc tatcgtgcag tgttagaagc
     2341 cttgaaggct ggttatcgtc atattgatac ggcggcgatt tatcagaatg aagaaagtgt
     2401 tggtcaagca atcaaagata gcggagttcc acgtgaagaa atgttcgtaa ctaccaagct
     2461 ttggaatagt cagcaaacct atgagcaaac tcgtcaagct ttggaaaaat ctatagaaaa
     2521 actgggcttg gattatttgg atttgtattt gattcattgg ccgaacccaa aaccgctcag
     2581 agaaaatgac gcatggaaaa ctcgcaatgc ggaagtttgg agagcgatgg aagacctcta
     2641 tcaagaaggg aaaatccgtg ctatcggcgt tagcaatttt cttccccatc atttggatgc
     2701 cttgcttgaa actgcaacta tcgttcctgc ggtcaatcaa gttcgcttgg cgccaggtgt
     2761 gtatcaagaa caagtcgtag cttactgtcg tgaaaaggga attttattgg aagcttgggg
     2821 gccttttggt cagggagaac tatttgatag caagcaagtg caagaaatcg cagcaaatca
     2881 cggaaaatcg attgctcaga tagccttggc ctggagcttg gcagaaggat ttttaccact
     2941 tccaaaatct gtcacaacct ctcgtattca agctaatctt gattgctttg gaattgaact
     3001 gagtcatgag gagagagaaa ccttaaaaac gattgctgtt caatcgggtg ctccacgagt
     3061 tgatgatgtg gatttctaga aaatcataaa aagaattgta cattattcta atttttgata
     3121 taatagtcag caggaaagaa agtcttatgg cgttcttcaa gcgagcttgg gatagtggga
     3181 gccaagtagg gcaaaataat gagctggcgc tttctgtagt attttcaaaa acaatgaagt
     3241 aataaattag ggtggaaccg cgtttctgac gcccctaggt taaatcaacc taggattgtc
     3301 ggacgcggtt cttttgctta ttcagtctat tgtgtgaaag aaaggagagc cgtggacaac
     3361 ctttatcttg taaaagacga tagtcaacta gctacatttc gtgattttgt agtaagaaat
     3421 actgaaaagt tgaaagatta tcaatctttt ttaaagaatg aacttgcagt ctgtgattta
     3481 ccgcaagccg ttatttggtc agattttaat gctgctacac agattattag ggaaagcgct
     3541 gttccagcct atacaaataa tagacgaatg gttatggcgc ctgatttagc tgtttggaaa
     3601 gagttgtatt tgtatcaatt gatggactac gagtgttctc agcaaactca agcaatagaa
     3661 agtcactatc attctttatc tgaaaatttc ctcttacaga ttgtaggaca tgagttagct
     3721 cattggtcgg agcatttttt agatgatttt gatggttatg actcttatat ctggttcgaa
     3781 gaggggatgg ttgaatatat tagtcgcaag tatttcttga cagaagagga atttcaagcg
     3841 gaaaaaattt gtaatcaatc tctcgtagaa ctttttcaga agaagtatgg ttggcattca
     3901 ttgaatgatt ttggttcttc gacttatgat aagaactatg caagtatttt ttatgaatac
     3961 tggcgcagct ttttgacagt agataagttg gtagaaaatt taggtagtgt acaagcggtc
     4021 ttagattctt atcatttatg ggcaaataca gaaaaaactt ttcccttgtt agattggttt
     4081 gttcagcaga aattaattga aaaagaaata taaaaactaa aggagtaaac aatgtctaag
     4141 aaattgacat ttcactgcgt cagtggcaga aacctcctta cagtcggact gccccacgct
     4201 cagcactaga gtgcctgagc tagacgcagt actaactcgt cttgcctcgt atgatcgacg
     4261 aggcagactc atgtcgcaag taattatttt ttattaagga gtaaacaatg tctaagaaat
     4321 taacatttca ctgcatcagt ggcagaaacc tccttacagt cggactgccc cacgctcagc
     4381 accctagtgc tgagctagac gcagtactaa ctcgtcttgc ctcgtataat cgacgaggca
     4441 gactcgtgtc gcaagaaatc attttttatt aaggagtaaa caatgtctaa gaaattaaca
     4501 tttcaagaaa ttattttgac tttgcaacaa ttttggaatg accaaggttg tatgcttatg
     4561 caggcttatg acaatgaaaa aggtgcgggg acaatgagtc cttacacttt ccttcgtgct
     4621 atcggacctg agccatggaa tgcagcttat gtagagccat cacgtcgtcc tgctgacggt
     4681 cgttatgggg aaaaccctaa ccgtctctac caacaccacc aattccaggt ggtcatgaag
     4741 ccttctccat caaatatcca agaactttac cttgagtctt tggaaaaatt gggaatcaat
     4801 cctttggagc acgatattcg ttttgttgag gacaactggg aaaacccatc aactggttca
     4861 gctggtcttg gttgggaagt ttggcttgac ggaatggaaa tcactcagtt cacttatttc
     4921 caacaagtcg gtggattggc aactggccct gtgactgcgg aagttaccta tggtttggag
     4981 cgcttggctt cttacattca agaagtagac tctgtctatg atatcgagtg ggctgatggt
     5041 gtaaaatacg gagaaatctt tatccagcct gagtatgagc actcaaaata ttcatttgaa
     5101 atttcggacc aagaaatgtt gcttgaaaac tttgataagt ttgaaaaaga agctggtcgt
     5161 gcattagaag aaggcttggt acaccctgcc tatgactatg ttctcaaatg ttcacatacc
     5221 tttaatctgc ttgacgcgcg tggtgccgta tctgtaacag agcgtgcagg ctatatcgct
     5281 cgtatccgta acttggcccg tgttgtagcc aaaacctttg ttgcagaacg caaacgccta
     5341 ggttacccac ttttggatga agaaacacga gttaaacttt tagcagaaga cgcagaatag
     5401 tgagtaatac tgtactctaa aatcgttaga ggcaagtcga agacttgcta gagggatatg
     5461 caccgccgta ctgttatgtg gggatttttg aaaccttagg ttcaaaaatc agtcagctaa
     5521 atccactttg atgagattgt tggaaatctt gataaattta gcattggatt tccatacaga
     5581 ctcacagagt tagttagcga cgtccccagt acctaaggtg catatcaaaa aagattgaag
     5641 aaaatgtaaa ggaaaaaaca tgacaaaaaa cttattagta gaactcggtc ttgaagaatt
     5701 accagcctat gttgtcacac caagtgaaaa acaactaggc gaaaaaatgg cagccttcct
     5761 caaggaaaac cgcctgtctt ttgaagccat tcaaaccttc tcaacaccac gtcgtttggc
     5821 tgttcgtgtg actggtttat cagacaaaca gtctgatttg acagaagatt tcaagggacc
     5881 agcaaagaaa attgccttgg atagtgatgg aaactttacc aaagcagctc aaggatttgt
     5941 ccgtggaaaa ggcttgactg ttgaagatat cgaattccgt gaaatcaagg gtgaagaata
     6001 tgtctatgtc actaaggaag aagttggtca agcagttgaa gccattgttc ctggtgttgt
     6061 agatgtcttg aagtcattga ctttccctgt cagcatgcac tgggctggaa atagctttga
     6121 atacattcgt cctgtccaca ctttaactgt tctcttggat gagcaagagt ttgacttgga
     6181 tttccttgat atcaagggaa gtcgtgtgag ccgtggccat cgtttcttgg ggaaagaaac
     6241 caaaattcag tcagcattga gctatgaaga agaccttcgt aagcagtttg taatcgcaga
     6301 tccatgtgaa cgtgagcaaa tgattgttga ccaaatcaag gaaattgagg caaaacatgg
     6361 tgtacgtatc gaaattgatg cggatttgct gaatgaagtc ttgaatttgg ttgaataccc
     6421 aactgccttc atgggaagtt ttgatgctaa ataccttgaa gttccagaag aagtcttggt
     6481 gacttctatg aaggaacacc agcgttactt tgttgttcgt gatcaagatg gaaaactctt
     6541 gccaaacttc atttctgttc gtaacggaaa cgcagagcgt ttgaaaaatg tcattaaagg
     6601 aaatgaaaaa gtcttggtag cccgcttgga agacggagaa ttcttctggc gtgaagacca
     6661 aaaattggtg atttcagatc ttgttgaaaa attaaacaat gtcaccttcc atgagaagat
     6721 tggttccctt cgtgaacaca tgattcgtac gggtcaaatc actgtacttt tggcagaaaa
     6781 agctggtttg tcagtggatg aaacagttga ccttgctcgt gcagcagcca tttacaagtt
     6841 tgacttattg acaggtatgg ttggtgaatt tgacgaactc caaggaatta tgggtgaaaa
     6901 atacaccctt cttgctggtg aaactccagc ggtggcagct gctattcgtg aacactacat
     6961 gcctacatca gctgaaggag aacttccaga gagcaaggtc ggcgcagttc tagccattgc
     7021 agacaaattg gatacgattt tgagtttctt ctcagtagga ttgattccat caggttctaa
     7081 tgacccttat gcccttcgtc gtgcaactca aggtgtggtt cgtatcttgg atgcctttgg
     7141 ttggcacatt gctatggatg agctgattga tagcctttat gcattgaaat ttgacagttt
     7201 gacttatgaa aataaagcag aggttatgga ctttatcaag gctcgtgttg ataagatgat
     7261 gggctctact ccaaaagata tcaaggaagc agttcttgca ggttcaaact ttgttgtggc
     7321 agatatgttg gaagcagcaa gtgctctcgt agaagtaagc aaggaagaag attttaaacc
     7381 atctgttgaa tcactttctc gtgcctttaa cctggctgag aaggcagaag gggttgctac
     7441 agttgattca gaactatttg agaatgacca agaaaaagct ttggcagaag cagtagaaac
     7501 actcgtttta tcaggacctg caagtcagca attgaaacaa ctctttgcgc ttagcccagt
     7561 cattgatgct ttctttgaaa atactatggt aatggctgaa gatcaggctg tccgtcaaaa
     7621 tcgtttggca atcttgtcac aactaaccaa gaaagcagct aagtttgctt gttttaacca
     7681 aattaacact aaataaaatt tgataaacgg actttatctt attacaaagg agaagaaatg
     7741 gatccgaaaa aaattgctcg tatcaatgag cttgctaaaa agaaaaaaac agaaggctta
     7801 acaccagaag aaaaagtgga acaagccaaa ctacgtgagg agtacatcga aggttatcgc
     7861 cgcgctgttc gtcaccacat tgaaggaatc aaaattgtgg acgaagaagg aaacgatgtt
     7921 acaccagaaa aactacgcca agtacaacgt gaaaaaggat tacatggccg tagtcttgat
     7981 gacccaaatt cataatatgt actctttctt ttaacaaaga tagatgaaac gataacaaag
     8041 agactagcag tttgtgtttg ctagtctttt ttcgctaaaa aaggaaccat aatggttcct
     8101 aaaaactat
//
LOCUS       DAVBCV010000075         7490 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBCV010000075 DAVBCV010000000
VERSION     DAVBCV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.075"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..1039
                     /locus_tag="VQ516_001905"
     CDS             <1..1039
                     /locus_tag="VQ516_001905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2512295.1"
                     /translation="IKVMDERFGHSIYIYESETLSDNQDEIPIKLTNHSLIEPFELLT
                     EMYALPKYYEKDPTPVLAPFYFTFFGMMVADLGYGLLLFLGTMLALKIFHLPSETKRF
                     LKFFNILGVAVAIWGGIYGSFFGYELPFHLISTTSDVMIILVVSVVFGFITVFAGLLA
                     SGLQKVRMKKYAEAYNSGFVWCVILLGLLFIAVGMLMPDMRLLFVLGKWVSIFNAVGI
                     LVVSIIQAKSLSGIGAGLFNLYNISSYIGDLVSFTRLMALGLSGASIASAFNLIVGLF
                     PGTLAKLTIGLVLFILLHAINIFLSLLSGYVHGARLIFVEFFGKFYEGGGKPFQPLKA
                     SEKYIKVITKN"
     gene            1055..1531
                     /locus_tag="VQ516_001906"
     CDS             1055..1531
                     /locus_tag="VQ516_001906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET2512296.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1565..2146
                     /locus_tag="VQ516_001907"
     CDS             1565..2146
                     /locus_tag="VQ516_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512297.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSVIVKK
                     KEVEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            2211..3218
                     /locus_tag="VQ516_001908"
     CDS             2211..3218
                     /locus_tag="VQ516_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET2512298.1"
                     /translation="MDTNLFSKINTTISVKENDFITEDKFQKIIQSKDTETMAFILEA
                     TPYHLSIDVLENPSQTETSLMTKLVNDYRWAYAESPSNKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKILDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFGSIKERIRPHYG
                     S"
     gene            3208..3528
                     /locus_tag="VQ516_001909"
     CDS             3208..3528
                     /locus_tag="VQ516_001909"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET2512299.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            3591..5366
                     /locus_tag="VQ516_001910"
     CDS             3591..5366
                     /locus_tag="VQ516_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET2512300.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGIPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGIYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            5367..6752
                     /locus_tag="VQ516_001911"
     CDS             5367..6752
                     /locus_tag="VQ516_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET2512301.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLSSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            6775..7386
                     /locus_tag="VQ516_001912"
     CDS             6775..7386
                     /locus_tag="VQ516_001912"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET2512302.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNRLRKEVESYLIDNLKSFAVAKSLKNSLMVEELFSIPSKEIELFIEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         2458 a         1042 c         1525 g         2465 t
ORIGIN      
        1 tataaaagtt atggatgagc gttttggaca ttctatttat atttatgaat cggaaacatt
       61 gtcggataat caagatgaaa tacctatcaa attaacgaat cattctttaa ttgaaccatt
      121 tgaattattg acagaaatgt atgctctgcc caaatattat gagaaagatc ctacacctgt
      181 attagcacca ttttacttta cattttttgg aatgatggtt gctgatttag gctatggttt
      241 actattgttt ttaggaacaa tgttagcatt aaaaattttt catctacctt cagaaactaa
      301 gagattttta aaattcttta atatattagg ggtagccgtt gcaatttggg gtggaatcta
      361 tggctcattt tttggatatg agttgccatt tcatctgata tctacaacct ctgatgtcat
      421 gattatatta gtagtgtcag ttgtgtttgg gtttattaca gtatttgcag gtttgttagc
      481 ttcaggacta caaaaagtaa gaatgaagaa atatgcagaa gcatataatt caggatttgt
      541 gtggtgtgtt attctgcttg gcttgttatt tattgctgtc ggaatgttga tgcctgatat
      601 gagactgtta tttgtattag ggaaatgggt atctattttt aatgctgtgg gaattttggt
      661 tgtttctatt attcaagcca aaagcttgtc aggtattgga gcaggattgt ttaatctata
      721 taacatttca tcttatatag gtgatttagt tagtttcact cgattgatgg cattaggatt
      781 atctggagca agtatagcat cagctttcaa tttaattgtt ggtttgtttc cgggaacatt
      841 ggctaaacta acaattggat tagtattatt cattctttta catgcgatca atatttttct
      901 atcgttacta tcaggatatg ttcatggagc acgtctgata tttgttgaat tttttggtaa
      961 gttttatgag ggtggaggaa aaccatttca acctttgaag gcttctgaga aatatattaa
     1021 ggttattaca aagaattaat ggaggatata tataatggaa catttagcaa cttatttttc
     1081 aacctatgga ggagctttct tcgctgcatt gggaattgta ttggcggttg gattaagtgg
     1141 tatggggtct gcttatggag ttggtaaggc tgggcaatct gccgcagctt tactgaaaga
     1201 acagccagaa aagtttgcct ctgctttgat attgcaatta ctgcccggaa cacaaggatt
     1261 atatggtttt gttattggaa ttttgatttg gttgcaatta actccagaac ttcctttaga
     1321 aaaaggcgtt gcttatttct ttgtagctct tccaattgct attgtaggat acttttcggc
     1381 taagcatcaa ggaaatgtag cagtagcggg aatgcaaatc ttggctaaaa gaccaaaaga
     1441 attcatgaag ggagcaattt tggctgccat ggtagaaacc tatgcaattc ttgcctttgt
     1501 cgtatcattc attttgaccc ttcgtgtata agaaattaat ttgctattca aaggaggtgt
     1561 ctaaatgagc aatttagaaa acttacgaga gtctgttatt gaacaagctc atgaaaaagg
     1621 gcgtatgaaa ttattggatt ccaaaaagaa gattgatgat gaatttgaaa tgcaaaagtc
     1681 ggtcattgta aagaaaaaag aagtggaaca tgaacgaaag ttaaaagaat tacaacagaa
     1741 atatcaaata atttttcaac aattaaaaaa taaggaacgc caatcaacgt tagtatcaaa
     1801 acagaaaata ttaaaagaac tttttcaatc tgctttaata gaaatggaat cttggagtgc
     1861 agataaagaa atggagttca tctatcgaat tctggaacga tattcacaac aagaggtcat
     1921 agtaactttt ggggagcgga ctttagctaa attcaatctg gaacaattag agaaattgaa
     1981 attctctttt ccaaatcatt tatttagtga acagcctatc tcaaatgaat caggcttact
     2041 tatttcaata ggtaaaattg atgataacta tttgtataaa acattaattg gatcgatttc
     2101 taaggaagaa agttcaagta tcgcaaatca aatttttatc aattaaggat gaaattggtt
     2161 aatccttctt agaaatttgg agtattccaa taaaattaga aaggtatttt atggatacta
     2221 atcttttttc aaaaataaat acgacgattt cggtaaaaga aaacgatttt attacagaag
     2281 ataaatttca aaaaattata caatccaaag atacggagac aatggcattt atcttagaag
     2341 caactcccta tcatttatcg attgacgttt tagaaaatcc tagtcagaca gagacttcgc
     2401 taatgacaaa attagtcaat gattatagat gggcctatgc tgaaagtccg tctaataaaa
     2461 ttgtgacttt atttgcttta cgatatgttt atcataatat caaagtttta ttaaaatcta
     2521 aggcggcaat taagaaagat ttttctaaat tattaattcc aatagggatt tttgatatag
     2581 aaagtttaaa acatttagtt tcttccttac attcagatac acttcctgat tttatggttc
     2641 atgaagtaga atcaatttgg aatgagtatg aaacttttaa taatattcgt gtacttgatg
     2701 tcggagctga tctagcatat tttaaacatc tgaaactttt atctaatgag ttagatgagg
     2761 tgctgtctca ggttattgtc gcaatgattg acttttataa tattattact gtaaaacgtg
     2821 gtttatctca aaataagagt catggggata ttttacaatt actttcagat gaaggaagta
     2881 tttctgctaa agaatttata tacattgtag aaaatcaaga aatatttgtg tggttcaata
     2941 aaataaatcc aagcttagat tcaatctttt caacttatga attgaagatg caggacgcaa
     3001 caatttcatc ttctgagtta gaatttttat gtgatttatt attgtataaa attttagatc
     3061 aaggaaggta caatgtagat gggccgttag tacttgctag atatttattg ggatgtgagt
     3121 ttgaagtaaa gaatctcaga atgattatat cagctcttca aaatacaatt ccctttggat
     3181 caataaaaga aaggatacgc ccgcattatg gaagctaata agtataaaat tggcataatt
     3241 ggtagccgtg atattatttt accctttagc atgattgggt ttgatatatt tcctgcctat
     3301 caagaacaag aagctataaa tactctaaga aaattagccc aatctgatta tggtgtcatt
     3361 tatatcactg aagacattgc ttcaatgata ttagatacaa ttcgccatta tgattctcaa
     3421 gttgtgcctg ctattatttt attaccgact cataaacaag gtttaaattt aggattaaaa
     3481 cgtatagagg ataatgtgga gaaagcagta ggacacaata ttttataata atgtacaaaa
     3541 ttgtctgtaa tattattcta taatttttgg acttagtaag gagaataact ttgactcaag
     3601 ggaagattat aaaagtatcg ggacctctag ttattgcatc aggtatgcag gaggctaata
     3661 ttcaagatat ttgccgtgta ggtaagctag ggttaatcgg tgaaattatt gaaatgagaa
     3721 gagatcaggc atctatccaa gtctatgagg aaacatctgg tcttggtccg ggagaacctg
     3781 ttgttacaac tggagaacct ctctcggttg aattaggacc aggattgatt tctcaaatgt
     3841 ttgatggcat acaacgccca ttagatcgat ttaaattggc tactcataat gattttctag
     3901 ttcgtggggt agaagttcca agtttggata gagatattaa gtggcatttt gattccacta
     3961 tagcaattgg tcaaaaagtg agtacgggtg atattctagg aactgtcaag gaaaccgagg
     4021 tagttaatca taaaattatg gttccttatg gagtatctgg cgaagtcgtt tctatcgcat
     4081 ctggcgattt tacaattgat gaagttgtat atgaaataaa aaaattggac ggtagtttct
     4141 ataaaggaac gcttatgcaa aaatggcctg ttcgcaaggc gcgtcctgtt tctaaacgtt
     4201 taatcccaga agaaccatta atcacaggtc aacgagttat tgatacattc tttccagtaa
     4261 ccaaaggggg agcagcagct gttcctggac cgtttggagc aggaaagaca gttgtacaac
     4321 accaagtagc taaattttcc aatgttgata ttgttattta tgtcggttgt ggagaacgtg
     4381 gaaatgaaat gacggatgta ctgaatgagt ttcctgagtt gattgaccct aataccggac
     4441 aatcaattat gcaacggaca gttctgattg ctaatacttc aaatatgcct gttgctgctc
     4501 gtgaggcttc aatttataca ggaattacca tggctgagta ttttcgtgat atgggctact
     4561 ctgtcgccat tatggctgat tcgacttcac gttgggcaga agcactacgt gaaatgtcag
     4621 gacgtctaga agaaatgcct ggtgatgagg gttatcctgc ttatctggga agtcgtatcg
     4681 ctgaatatta tgaaagagcg ggacgttctc aggttctagg gattccagaa cgtgaaggaa
     4741 ctattactgc tattggagct gtatcgccac ccggtggaga tatttcagaa ccagttactc
     4801 aaaacacttt acggattgtg aaagtttttt gggggcttga tgctccgttg gcacagagac
     4861 gtcattttcc tgcaattaac tggcttacat cttattcact ctataaagac agtgtgggaa
     4921 tttatataga tggtaaagag aagacagatt ggaatagtaa aataactcgt gcgatgaact
     4981 acttacaacg ggaatctagt ttagaggaaa ttgttcgtct tgttggaatt gattctctgt
     5041 ctgataatga acgactaacg atggaaattg ctaaacaaat tcgagaagat tatttgcaac
     5101 agaatgcttt tgattcagta gatacattca cttcgtttgc aaaacaagaa gcaatgctaa
     5161 gtaatatcct aacttttgct gatcaggcaa atcatgcttt agagttaggt tcttacttta
     5221 cagagattat ggaaggtacc gtggcagttc gagaccgtat ggcgcgaagt aaatatgttt
     5281 cagaagatag attagatgaa atcaaaatta tttcaaatga gattacacat caaattcatt
     5341 tgatattaga aacaggaggt ctataaatga gtgttataaa agaatacaga actgctagtg
     5401 aagttgttgg gcctcttatg attgttgaac aagtaaataa tgtgtcttac aatgagttag
     5461 ttgaaattca acttcataat ggagaaattc gtcgtggaca agttttagag attcacgaag
     5521 ataaagcaat ggttcaactt tttgaaggat ctagtggaat aaatttagaa aagtctaaaa
     5581 ttcgttttgc tggtcatgca ctagaattgg ctgtatctga ggatatggtt ggtcgtattt
     5641 ttaatgggat gggaaaacca attgatggtg gaccagattt aattccagag aaatatttag
     5701 atattgatgg tcaagctatt aatcctgtat ctagagatta tccagatgaa tttattcaga
     5761 cagggatctc ctctattgat catttgaata ctcttgtacg tggtcaaaaa ttaccagtat
     5821 tttcaggttc gggcttacct cataatgaat tagcagctca gatagcaaga caagcgactg
     5881 ttttaaattc tgatgaaaat tttgcggttg tatttgcagc aatgggtatt acttttgaag
     5941 aagctgagtt ttttatggaa gaactcagaa aaacaggagc gatcgatcgt tcagtcttat
     6001 ttatgaactt ggcaaatgat cctgcaattg agcgtattgc aactccccgc attgctttaa
     6061 ccgcggcaga gtatctagct tttgaaaaag atatgcacgt tctagttatc atgacggata
     6121 tgactaacta ttgtgaagcg ttacgtgaag tctcggcagc gcgccgtgaa gttccaggta
     6181 gacgaggcta tccgggatat ttatatacaa atttatcaac tctatatgaa agggctggtc
     6241 gcttagttgg taaaaaaggt tcagtgacac agattcctat tttaacaatg ccagaagatg
     6301 acataacaca tccaattcct gatttaactg gatacattac tgaagggcaa attattttgt
     6361 ctcatgagtt gtataatcaa ggttatcgtc caccaatcaa tgttttatct tctctctctc
     6421 gattaaaaga taagggatct ggagaaggta aaactcgtgg agatcatgct ccaactatga
     6481 atcaactgtt tgcagcctat gcccaaggga aaaaggttga agagttagca gtagtattag
     6541 gagaatcggc tttatctgat gtagataaat tgtatgtgag atttacaaag cgttttgaag
     6601 aagagtacat caaccaagga ttttataaaa atcgaaatat agaagatacg ttgaatcttg
     6661 ggtgggaatt actatcaatt cttcctagaa cagagttaaa acgtatcaaa gatgatttgc
     6721 ttgataaata tttacctttg gtagaagttt aatccagaaa tggagtgatt atctatggta
     6781 cgtttgaatg taaaaccaac tcgtatggaa ttgaataact taaaggaacg tttgaaaaca
     6841 gctgaacgtg gacataagtt attaaaggat aaaagagatg aattgatgag gcgatttatt
     6901 tctttgattc gtgagaataa tcgacttcgg aaagaagtag aaagttatct aattgataat
     6961 ctaaaatctt ttgcagttgc taaatcatta aagaactctc taatggtgga ggaattattt
     7021 tcaattccat cgaaagaaat tgaattattt attgagaaag aaaatatcat gagtgtaaca
     7081 gttcctagaa tgcatatgaa tattacttct caaaatgaga acagtgaata cagctattta
     7141 tcttctaata gtgaaatgga tgatgtattt gctacaatga atagtttaat tgataaatta
     7201 ctaagactgg cagaagttga aaaaacgtgt cagttaatgg ctgatgaaat agaaaaaaca
     7261 cgtagacgtg taaatggttt agaatattcg attattccaa acttgtcgga aactattcat
     7321 tatatagaat tgaaactaga ggaggcagaa agagccaatt tagttcgtat tatgaaagtg
     7381 aagtagttcc tttatttaga tttttaatta gatgaacaaa tatcagcttg gataaggcgg
     7441 taagcctttc taagcttttt taggctcttt gtcaactgta gtgggttgaa
//
LOCUS       DAVBCV010000076         7474 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBCV010000076 DAVBCV010000000
VERSION     DAVBCV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.076"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            60..929
                     /locus_tag="VQ516_001913"
     CDS             60..929
                     /locus_tag="VQ516_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2512303.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            933..1889
                     /locus_tag="VQ516_001914"
     CDS             933..1889
                     /locus_tag="VQ516_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2512304.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1889..2653
                     /locus_tag="VQ516_001915"
     CDS             1889..2653
                     /locus_tag="VQ516_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2512305.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2653..3363
                     /locus_tag="VQ516_001916"
     CDS             2653..3363
                     /locus_tag="VQ516_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2512306.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3828..4484
                     /locus_tag="VQ516_001917"
     CDS             3828..4484
                     /locus_tag="VQ516_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2512307.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALREKFEANGIQVEEIARTSAKVL
                     "
     gene            4592..5687
                     /gene="prfB"
                     /locus_tag="VQ516_001918"
     CDS             join(4592..4663,4665..5687)
                     /gene="prfB"
                     /locus_tag="VQ516_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2512308.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5705..6397
                     /gene="ftsE"
                     /locus_tag="VQ516_001919"
     CDS             5705..6397
                     /gene="ftsE"
                     /locus_tag="VQ516_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2512309.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6390..7316
                     /locus_tag="VQ516_001920"
     CDS             6390..7316
                     /locus_tag="VQ516_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2512310.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         2208 a         1292 c         1682 g         2292 t
ORIGIN      
        1 agaacctatc aaaaagtgag ggaaaaccct cggaattata aatagaaaga gtgaatctta
       61 tgctccaaca actcgtaaat ggtttgattc taggtagtgt ttacgcgctg ttagccctag
      121 gatataccat ggtttacgga attatcaagc tcatcaactt cgcccatggt gatatttata
      181 tgatgggagc ctttatcggt tatttcttga tcaattcttt ccaaatgaat ttctttgtag
      241 cgcttattgt agctatgcta gcgacagcta ttcttggtgt cgtgattgag tttcttgctt
      301 accgaccttt gcgccactct actcgtattg ctgttttgat tacggctatt ggggtttctt
      361 tcctattgga gtatggaatg gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag
      421 cgattcaaac agttcgatat gatttgggac caattagctt aacaaatgtg cagttaatga
      481 ttttggccat ttccttgatt ttgatgattt tgttacaagt cattgtccaa aagactaaga
      541 tggggaaagc catgcgtgca gtatcagtag atagcgacgc ggcgcaattg atggggatca
      601 atgtaaaccg tacgattagc tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg
      661 gtgttctgat tgctctttat tataactctc ttgagccttt gatgggggtt actccaggtc
      721 ttaaatcttt cgttgccgca gtacttggtg gtatcggaat tattcctggt gcggctcttg
      781 gtggctttgt gattggtcta ttggaaacct ttgcgactgc ctttgggatg tcagatttcc
      841 gtgatgccat tgtttatgga atcttgttgt tgatcttgat tgtccgccca gctggtatcc
      901 ttggtaagaa tgtgaaagag aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc
      961 tatggttact ccttttgtta gctggctata gcttgattag tgtactggtt tcagtcggag
     1021 tacttaatct attctatgta cagattttac aacaaattgg aattaatatt attttggctg
     1081 ttggtctcaa cttaatcgtt ggtttttcag gacaattttc acttggtcat gctggtttca
     1141 tggcgattgg tgcctatgca gcagctatta ttggttctaa attaccaacc tacggtgcct
     1201 tctttggagc tatgcttgta ggggctttgc tttcaggagc agttgcctta cttgtcggca
     1261 ttccaacctt gcgcttgaag ggggactatc ttgcggtagc aactctaggt gtttctgaaa
     1321 ttatccgtat ctttatcatc aatggtggaa gtcttacaaa tggtgcggca ggtatcttag
     1381 ggattcctaa ctttacaact tggcaaatgg tttacttctt tgtcgtgatt acaaccattg
     1441 caaccttgaa cttcttgcgt agcccaattg gccgttcaac cctctctgtt cgtgaagatg
     1501 aaatcgctgc tgagtcagtt ggggttaata cgactaaaat taaaatcatc gcttttgtct
     1561 ttggtgccat tactgcaagt attgctgggt cacttcaggc aggatttatc gggtctgttg
     1621 taccgaaaga ttacaccttc attaactcaa tcaacgtttt gattattgtt gtatttggtg
     1681 gactcggttc cattacaggt gcgattgttt cggctattgt tctgggaatt ttgaatatgc
     1741 ttctccaaga tgttgctagt gtgcgtatga ttatttacgc tttagccttg gtattggtaa
     1801 tgattttcag accaggtgga ctccttggaa cgtgggaact gagcctatca cgtttcttta
     1861 aaaaatctaa gaaggaggaa caaaactaat ggcattactt gaagtaaaac agttaaccaa
     1921 acattttggt ggtctaacag ctgttggaga tgtgactctt gaattgaacg aaggggaact
     1981 ggttggatta atcggtccaa acggagctgg gaaaaccacc cttttcaacc ttttgaccgg
     2041 tgtttatgaa ccaagcgagg gaacagtaac cctagatggt caccttttga atgggaaatc
     2101 accttataag attgcctctt tgggacttgg acgtactttc caaaatatcc gtctctttaa
     2161 agatttaaca gttttagata atgttttgat tgcttttgga aaccatcaca aacagcatgt
     2221 ttttactagt ttcttacgct taccagcttt ttacaagagt gaaaaagaat taaaggctaa
     2281 agctttggaa ttgttgaaaa tctttgattt agatggtgat gcagagactc ttgctaaaaa
     2341 tctttcctac ggacaacaac gtcgtttgga aattgttcgt gcccttgcta cggaacctaa
     2401 aattctcttc ttagatgaac cagcagcagg tatgaaccca caggaaacag ccgaattgac
     2461 tgagttaatt cgtcgtatca aagatgaatt taagattaca atcatgttga ttgaacacga
     2521 tatgaatctg gtcatggaag taacagaacg tatctacgta cttgaatatg gccgtttaat
     2581 tgctcaagga actccagacg aaattaagac caataaacgc gttatcgaag cttatctagg
     2641 aggtgaagcc taatgtctat gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc
     2701 caagcagtcc gtgatgtaag ctttgaagtt aatgaaggag aagttgtttc ccttatcggt
     2761 gccaacggtg caggtaagac aactattctt cgcaccttgt caggtttggt tcgaccaagt
     2821 tcaggaaaga ttgaattttt aggtcaagaa atccaaaaaa taccagctca gaaaatcgtg
     2881 gcaggtggtc tttcacaagt tccagaagga cgccacgtct ttcctggctt gactgttatg
     2941 gaaaatcttg aaatgggagc tttcttaaag aaaaatcgtg aagaaaatca agctaacttg
     3001 aagaaggttt tctcacgctt tcctcgtctt gaagaacgta agaaccaaga tgcagctact
     3061 ctttcaggag gggaacaaca aatgcttgcc atgggacgcg ctcttatgtc aacaccaaaa
     3121 cttcttcttt tagatgaacc atcaatggga cttgccccaa tcttcatcca agagattttt
     3181 gatatcattc aagatattca gaagcaagga acaaccgtcc tcttgattga acaaaatgcc
     3241 aataaagcac ttgcaatctc tgaccgagga tatgtattgg aaacaggaaa aatcgtccta
     3301 tcaggaacag gaaaagaact cgcttcatca gaagaagtca gaaaagcata tctaggtggc
     3361 taaaacaatc cagtggattg ttttagtcgg cagatgaaga ttacgaagta atcatcaata
     3421 tagtccgggg gcccttttta gtcggtagat tgagattgcg aagcaatcat caaatccaga
     3481 ggattgtttt agccggcaga taaagattac gaagtaatca tcaatatagt ccgggggccc
     3541 tttttagtcg gtggatgaag attgcgaagc aatcatcaaa tccagaggat tgttttagcc
     3601 ggtagattga gattgcaaac aaatctgcat ctacattgaa agcttaattt ctaataattg
     3661 aaaaaatcga atgaaaagtt tcttaccttc attcacagag ctcgatttta gagctctttt
     3721 tgctagctta ttcatacttt tctaaatttt gaaaaagaaa tgtaagcgtt tgatagattt
     3781 gcaaaaagat tgtataatag ggataagaat agaaaaggag aagtctcatg gcagttaaag
     3841 attttatgac ccgcaaggta gtttatatta gtccagatac aacagtatct catgcagcag
     3901 atttgatgag agagcaaggt ttgcaccgtc tgcctgttat cgaaaatgat caattggttg
     3961 gtttggtgac tgagggaacc attgcacaag caagtccatc taaagcaaca agtctttcta
     4021 tctatgagat gaattatctt ctgaataaga caaaagtaaa agatgtcatg attcgcgatg
     4081 ttgtcactgt ctcaggctat gctagtctag aagatgcaac ttatctgatg ttgaaaaata
     4141 agattggtat tctccctgtc gtagataacc atcaagtata cggagttatt actgaccgtg
     4201 acgttttcca agcctttctt gaaattgcag gttatggcga agaagggatt cgtgtacgct
     4261 ttgttacaga agatgaagtt ggtgttcttg gaaaaattgt ttctttgatt gtagaagaaa
     4321 atttgaatat ctcccataca gtcaatattc cgcgtaagga tggtaaggtg attatcgaag
     4381 tgcaaatcga tgaatcaatt gatttaccag ccttgagaga aaaatttgaa gcaaatggta
     4441 ttcaagtgga agaaatcgct cgcacttcag caaaagtctt gtaagaaggg aagtccaaag
     4501 gcttcttttt tcatgaaaag gggattagag caaaagatgg aaagaaatga taaaatatgc
     4561 tataatgaaa taatgtaaaa aaggagtatt tatggacatt tcagtaattc gtcagaaaat
     4621 tgacgcaaat cgtgaaaaat tagcttcttt cagggggtct ctttgacctc gaagggctag
     4681 aggaagagat tgccatcttg gaaaacaaga tgacagaacc tgatttttgg aacgataata
     4741 ttgcggccca aaaaacgtcg caagaattga atgaattaaa aaacacttac aataccttcc
     4801 ataagatgga agagttgcag gatgaagtcg aaattttatt ggattttttg gctgaagacg
     4861 agtcagtgca tgatgaactg gtagcgcagt tagccgaact tgataagata atgaccagct
     4921 acgagatgac tctactcttg tcagaacctt atgaccacaa caatgccatc ttggaaatcc
     4981 atccaggttc tggtggtact gaggcgcagg actggggtga tatgttgctt cgtatgtata
     5041 ctcgttatgg taatgctaaa ggctttaaag tggaagtgtt ggattaccaa gcaggtgatg
     5101 aggctggtat taagtcggta actttatcat ttgaagggcc taatgcctat ggtctcctca
     5161 agtcagaaat gggtgttcac cgcttagtgc gaatctcacc atttgactct gccaaacgtc
     5221 gccatacctc tttcacatct gtagaagtga tgccagaatt ggatgatact attgaagtgg
     5281 aaatccgtga agatgatatc aagatggata ccttccgttc aggtggtgcc ggtggacaaa
     5341 acgtcaataa ggtttcaaca ggtgtacgtt taacccacat tccaactgga attgttgtcc
     5401 aatcaacagt ggatcgtacc cagtatggaa atagagatcg tgccatgaag atgttgcagg
     5461 ctaagctcta tcaaatggag caagataaga aggctgcgga ggtagattct ctcaaaggtg
     5521 agaaaaagga gatcacttgg ggaagccaaa tccgttctta tgtcttcacg ccttatacta
     5581 tggtaaaaga tcaccgaact agctttgagg ttgctcaggt agataaggtt atggatgggg
     5641 acctagatgg ttttatagat gcttatctca agtggcgaat tagctaagat agaaaggaac
     5701 tcacatgtca attattgaaa tgagagatgt cgttaaaaaa tacgacaacg gaacgactgc
     5761 tctacgcggt gtttcggtca gcgttcaacc gggggaattt gcttacatcg taggaccttc
     5821 aggagcaggg aagtcaactt ttattcgttc tctgtatcgt gaagtaaaaa tcgataaagg
     5881 aagtctatca gttgctggtt ttaatctggt taagatcaaa aagaaagatg tcccgcttct
     5941 acgtcgtagt gttggggttg tcttccaaga ttataaattg ttaccaaaga aaactgtcta
     6001 tgaaaatatt gcttacgcta tggaagtaat cggggaaaat cgccgtaata tcaaaagacg
     6061 agtgatggaa gttttggact tggttggatt gaagcataag gttcgttctt tcccaaatga
     6121 actctcaggt ggggagcagc agcgaattgc gattgcgcgt gcaattgtaa ataatcccaa
     6181 agtattgata gctgatgagc caacaggaaa tctggatccg gataattcat gggaaattat
     6241 gaatctcttg gaacggatta acctacaagg gacaactatt ttgatggcga ctcataatag
     6301 ccagattgta aataccttgc gccaccgtgt cattgccatt gaaaatggcc gtgtcgttcg
     6361 tgacgaatca aaaggagagt atggatacga tgattagtag attttttcgc catttatttg
     6421 aagccttaaa aagtttgaaa cgaaatggtt ggatgacagt agctgctgtc agttcagtca
     6481 tgattacttt gaccttggtg gcaatatttg catctgttat tttcaataca gcgaaactag
     6541 ctacagatat tgaaaataat gtccgtgtag tagtttatat ccgaaaggat gtggaagata
     6601 atagtcagac aattgaaaaa gaaggtcaaa ctgttacaaa taatgactac cacaaggtat
     6661 atgattcttt gaagaacatg tctacggtta aaagtgttac cttttcaagt aaagaagaac
     6721 aatatgaaaa attaaccgag ataatgggag ataactggaa aatctttgaa ggagatgcca
     6781 atcctctcta tgatgcctat attgtagagg caaacactcc aaatgatgta aaaactatag
     6841 ccgaagatgc taaaaaaatt gaaggtgtct ctgaggttca agatggcggt gccaatacag
     6901 aaagactctt caagttagct tcatttatcc gtgtttgggg actagggatt gctgctttgt
     6961 taatttttat cgcagttttc ttgatttcaa ataccattcg tattaccatt atttcccgca
     7021 gtcgcgaaat tcaaatcatg cgcttggttg gagctaaaaa cagttatatc cgtggaccgt
     7081 tcttgttaga aggagccttt atcggtttat tgggagctat cgcaccatct gttttggtct
     7141 ttattgttta tcaaattgtt taccaatctg tcaacaaatc gttggtaggg caaaatctat
     7201 ccatgattag tccagattta tttagtccgt tgatgattgc cctactattt gtgattgggg
     7261 ttttcattgg ttcattggga tcaggaatat ccatgcgccg attcttgaag atttaggtaa
     7321 aatagctgct tttatgagga gattgtaaaa tctccttttt tgctacaaga gtttttgaaa
     7381 agagatgcgt agaagaaaag agcttccaaa gaagtccccc agagaagact tcttgatggc
     7441 taatttgaac ttgaaatttg gcgcactaga aatt
//
LOCUS       DAVBCV010000077         7292 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBCV010000077 DAVBCV010000000
VERSION     DAVBCV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.077"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(115..390)
                     /locus_tag="VQ516_001921"
     CDS             complement(115..390)
                     /locus_tag="VQ516_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2512311.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(496..1335)
                     /locus_tag="VQ516_001922"
     CDS             complement(496..1335)
                     /locus_tag="VQ516_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2512312.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1477..2325
                     /locus_tag="VQ516_001923"
     CDS             1477..2325
                     /locus_tag="VQ516_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2512313.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2418..3335)
                     /locus_tag="VQ516_001924"
     CDS             complement(2418..3335)
                     /locus_tag="VQ516_001924"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2512314.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3772..3977
                     /locus_tag="VQ516_001925"
                     /pseudo
     CDS             3772..3977
                     /locus_tag="VQ516_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="VQ516_001926"
     CDS             complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="VQ516_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2512315.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4598..4942)
                     /locus_tag="VQ516_001927"
     CDS             complement(4598..4942)
                     /locus_tag="VQ516_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2512316.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="VQ516_001928"
     CDS             complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="VQ516_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2512317.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5308..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5490..6089)
                     /locus_tag="VQ516_001929"
     CDS             complement(5490..6089)
                     /locus_tag="VQ516_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2512318.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            6559..6858
                     /locus_tag="VQ516_001930"
     CDS             6559..6858
                     /locus_tag="VQ516_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2512319.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            7077..>7292
                     /locus_tag="VQ516_001931"
     CDS             7077..>7292
                     /locus_tag="VQ516_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512320.1"
                     /translation="MGQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCP
                     ECGSQMKKYDFQKPSKIPYLETTGMPTR"
BASE COUNT         2333 a         1473 c         1197 g         2289 t
ORIGIN      
        1 tacaatattt tcggaacatt caacttttta actctattta ttactagatt tcataattaa
       61 aaagcctact gaccaagcta gaaagcttga tacaataggc tttttaaaga ctgattattt
      121 aacagcgtct ttaagagctt taccagcttt gaatgctggt actttagaag ctgcaattgt
      181 catttcttta ccagtttgtg ggttgcgacc tttacgttct gcacgctcac gaacttcaaa
      241 gttaccaaaa ccgatcaatt gaactttttc accagctgca agatagtcag ctactgctgc
      301 aaatacagct tcaactgctg ctgctgagtc tttcttagtc aattctgtag cttctgctac
      361 tttagcgatc aaatcttgtt tgtttgccat gttaatgatt cctccagata atttctaatt
      421 aacaaatata atcatatcct aaaatccctt ttaggtcaag ttaaaaacgc tatttcttcc
      481 aattttattt attttttagg agtggtaacg tatcaaaata gcccaagcgt tctcacccgt
      541 gtgagtttga ataatggaac ccgtttccaa aacagaaatt ggcttttcaa cataagcttg
      601 taagctttct ttcatctctt ttgcccaatc atcactacca gaatatgaaa ttccaatctc
      661 tgctacagca cgttcagaaa gcgatgttat caactcatct aaccattttt taaatgtttt
      721 agttccacga cctttaacca ttggctgcaa ttcatggtct ttcatttgca tgacaacacg
      781 gatattgaga agggagctca acaagccagt tacacggcca attcgtccac ctttgacaag
      841 attttccaaa gttgaaacac caatataaag ctctgtatgg tttttaacct cttctacatg
      901 agataaaatt gcctccatat ctttaccttc ttgagctaac ttcgcagcct caacaacttg
      961 gaatttcagg gcttggtcag tgaaggaact atcaacaaca atcacatctg cagtagatag
     1021 gctagcacct tggcgtgctg cttctaccgt acccgaaaga gcatgggaca tatgaatagc
     1081 aagaatctgg ccaccatctt tgcataggtc ttcaaaaatc tcagcaaaga cacctacagg
     1141 tggctgactt gttttcggaa gattcttact ttcttgcatc aactgaagaa atttaccttc
     1201 ttctttcaaa tccgcatcag aataaacaac attatcaatc attacagata atggaacaat
     1261 tgtaatatct aattgcttta ctagttcagg ttcaatagta acagatgaat cggttacaat
     1321 cttaattttt gtcatagtat caatctttct attttaggat tcagattggt ttccttactt
     1381 ctaattatat caaaaaaaag attaaaaatc ctaatggagt caatcaaatt ttccgtaaaa
     1441 tttgatataa tcaacttata agaaaagagg tgtcctatga ttaaaaaaat ttaccccatt
     1501 tttaccattt tactaggtgc tgctatttat gcttttggac tgacttattt tgtagttccc
     1561 catcatctct ttgaaggagg ggcgacaggt attaccctca tcacatttta tctttttaaa
     1621 atccctgttt ccctcatgaa cctgctgatt aatattcccc ttttcatcct agcttggaag
     1681 atctttggag ccaaatccct ctattctagt ttactaggaa ccttagcttt gtccggctgg
     1741 ttagcttttt ttgagcatat tccccttcat attgatcttc aaggtgattt actaataaca
     1801 gcccttatag cgggaatcct attgggaatt ggccttggaa ttatttttaa tgctggaggt
     1861 acaactggcg gaactgatat tctagctcgt attctcaaca aatacactca tatatccata
     1921 ggaaaactgc tctttatctt agatttttgt attctcatgt tgattctcct aatcttcaag
     1981 gatttgagat tggtttccta cacgcttttg tttgatttta ttgtttctcg tgttattgat
     2041 ttgattggtg aaggaggata tgccggcaaa ggctttatga ttatcacaaa acgtcctgac
     2101 caacttgcta aggcgattaa tgatgacctc ggaagaggtg ttacttttat ttctggtcaa
     2161 ggctactata gtaaagaaaa tttgaaaatc atctactgta ttgtcggaag aaatgaaatt
     2221 gtgaaaacga aggaaatgat tcatcgaatc gatcctcaag cctttataac tattacagaa
     2281 gcccatgaaa tcctaggaga aggcttcacc tttgaaaaag aataaaaaga ggtaatgtcg
     2341 tgacctcaaa agttagacta aatcatctat cttttgggtt acagacaacc tcttttttgt
     2401 tttatttact caagctctta agaccaattc cgagttactt cttcatcagc ctttaactga
     2461 tccactaatt ggtcaactga gtcaaatttg gtcatatcac gaatgcgatc aagccaataa
     2521 accatgacgg tttccccata aatatcttga ttaaaatcaa aaatattgac ttcaaaacgt
     2581 gcttcttctc catcaaaggt cacatttttc ccgacactag ccatagcacg atacttctgt
     2641 ctttgaatct caacatcaac gacataaacg ccatctgctg gcatataagt acggtctaaa
     2701 agcactaaat tcgctgtcgg ataaccaatt gtacgaccac gagcattacc atgaaccacc
     2761 atacctcttg atggaagcgg tgcccccaaa agttttcctg cttctttcac atttccatct
     2821 aaaatagctt gacggatacg agttgaacta atctttcctt tctcatcttc tacaggtgga
     2881 acaatgataa cttctccatc aaagtaatcc tttaaatctt ctgctgtttt tttgtcagaa
     2941 ccaaatgtat aatcaaaacc tgcaacaata attttggcat tcatagcctt gatataagtt
     3001 gcaaagaatt cttgtgcagt gagactagcg aattgactac taaaatcaag gagatataat
     3061 tcttctacac cttcgcgctt taattttctt tcacgttcag cagggttcaa aatatgcaaa
     3121 aacaaatccg gatgataagg ctctaaagcg atctttggag attcattaaa ggtcataacg
     3181 acgataggta ataaatcctt tctcgcagcc ttgttggcaa cacgaaataa ttcttgatgc
     3241 cccttatgta tgccatcaaa atagccgaga acaacgactg aatcagatgg tgtgccaata
     3301 tctttttggt tttttatagg aatagtaata atcataaaat aattatatca tagcgatagc
     3361 tatttctgga acagaaaatc tgaaatgttg tttttttcac atgaagtgta cctgttttca
     3421 aaaagcactt tattctatcg ttgcttaact atgaactttg caatattctt ctcaaaaact
     3481 tgtaggacat cttcaaaatt ttgcaaggag tgattagact tgttcggtaa ccataaagtg
     3541 tcatactatg ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta
     3601 attggtgccg aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa
     3661 aatgagacaa cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag
     3721 tcactttccc tgatgacaaa gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct
     3781 ttccaacaac tcccatctag tgtgcttcag actgggctat ttttctctcc atctgttagc
     3841 ttggattctc agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc
     3901 aaaccattca gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt
     3961 atagtcaatt gaaataaaat ctgaagaaat cgagtaggaa actcatatca atttttaaca
     4021 gtgttctatt ccagattcat actcaatgaa aattaaagtg caaactagga agttagccgc
     4081 aggctgtact tgagtacggc aaggtgaaac tgacgtggtt tgaatttgat tttcgaagag
     4141 tatcaatcca ctatacatat taaaaagtat ggatatcaaa acaaaaaaac aggctctccg
     4201 aaaactcgga aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt
     4261 tcgggataaa aggttcaatg gaccttttta tttagcgatt gggtaaacag aaacttgttt
     4321 tttatcgcgt cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc
     4381 gtctccacca cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag
     4441 gattgatcca cctgttacag tttgtccgtc agctgcttta gctccaagac gttttgcttg
     4501 tgaatcacgt ccgtttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt
     4561 gttaagagtc atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg
     4621 tttggacgaa ctcagaatag ttctccgata agtttgccat acctaagaaa aatgattcaa
     4681 agaataactg ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca
     4741 gatagccacc ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa
     4801 tagaattgat aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt
     4861 attcgccact ctcggcgtgt ccagtaattt ccgcacccct cagctcgcca tcttcggctc
     4921 tctcaaagac tgcttgtatc atgtgttctc cttaaaatta agcgttaatt gcgttgatga
     4981 caacttttgt atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt
     5041 acttgtaagt aacaactttc ttttgttttc cttgtttttc aacagttcca actacagtag
     5101 ctccagcaac aagtggagtt ccgacaacag tgttttcacc accaacaaga acaacttcgt
     5161 taaaagtaac ttcttgacca gcttcaacgt tcaatttttc aatgtaaact gcttgaccaa
     5221 cttcaacttt aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc
     5281 tcctatgatt tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac
     5341 aaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5401 tagtcaactc ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat
     5461 caagcttttt cttttacttt tttctgccac taggcaaaaa gcatactgag ataaaaaatg
     5521 ctcatcataa taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct
     5581 gaaaagacat cgccaaggac aaaactacta agcaggctaa caaatatgaa cccttcacgc
     5641 aaaaaaggag tgtgcttagt tcggaaataa tctccaaaag ctagcattgt tcgtttgata
     5701 ttccctgtca taaaggcatt attgtaggca atacccgaaa cctcaccaaa agcagttgtc
     5761 accagtccca tacagaaggc caagggctgc actagataga tattatctac agtttgtggc
     5821 acaaaaccaa taattattga taggattgct aaaggaatca aggacagaat aggttttttc
     5881 acaattctca atttttcctt ataaatcgtt aataaaaaga ctcccattat aaacgttatt
     5941 aaggttaaaa gcttatccct aacatccgaa acattatttt gtattagctc taccgaaaga
     6001 aagacaacat ttccagcttg tccagctaca agggtattcc ctcgaacaat aaaagtgtaa
     6061 gcatccacat atccagcaca aaatgtcaaa aaagtgctaa ccttttagac tgacgtgata
     6121 tttttcttat aggtaataac ctcattttcc ctcccattgt attttctctt agaaatattg
     6181 taccatttct ttctaaaaaa tcgtagacta ccatttagat tttactatta gcataaaaat
     6241 aataatagac aactatttat ccaaaaatag atagaggtaa catgtttgca aacaaagcat
     6301 acgaaccttt agtaaaatca tttccatgaa actagaatag agcccctctt agcaaaaatc
     6361 attattttaa tttatttcta atcactcctt gacataaata actctcacca ataaaagact
     6421 atgtcttaaa aaaatggtat aataaaatca atacctgggc ttgatggcta tgctactaat
     6481 aacaattagg agagaaaatc aggcacttgt taacaacaag gattatcccc ttgagatgaa
     6541 aggaacttta gaaatcttat gatgaacatg caaaacatga tgcgtcaagc acaaaaactt
     6601 caaaaacaaa tggaacaaag ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct
     6661 gctcaagatc ttgtccaagc gaccttaact ggcgataaga aagttgtcag cattgatttc
     6721 aatccagctg tcgttgaccc agaggacctt gagactcttt ctgatatgac cgttcaagcc
     6781 atcaactctg ctcttgaaca aatcgatgaa actactaaga aaaaactggg tgctttcgct
     6841 gggaaattgc cattctaaaa acaaggagct agaacaatac ttgtcgataa caaaggctaa
     6901 gaaaggtgca aaaattataa aaaacgcata gtatcaagca acctaaaact ttgggctcta
     6961 taatatttgt agtgggtaaa tccactatag atattatgga gcctatttta ttgtagaaaa
     7021 aaatcccata agatctataa tgaaaagcga ccaaacaact cattagaaag attcatatgg
     7081 gacaattaca ttttatcaca aaattactag acattaaaga ccctaatatc cagattttag
     7141 acatcatcaa taaggataca cacaaggaaa tcatcgccaa actggactac gacgccccat
     7201 cttgccctga gtgcggaagt caaatgaaga aatatgactt tcaaaaaccg tctaagattc
     7261 cttaccttga gacaactggt atgcctacta ga
//
LOCUS       DAVBCV010000078         7220 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBCV010000078 DAVBCV010000000
VERSION     DAVBCV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.078"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            231..645
                     /locus_tag="VQ516_001932"
                     /pseudo
     CDS             231..645
                     /locus_tag="VQ516_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(684..1127)
                     /locus_tag="VQ516_001933"
     CDS             complement(684..1127)
                     /locus_tag="VQ516_001933"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2512321.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(1157..3379)
                     /locus_tag="VQ516_001934"
     CDS             complement(1157..3379)
                     /locus_tag="VQ516_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2512322.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            3790..4032
                     /locus_tag="VQ516_001935"
     CDS             3790..4032
                     /locus_tag="VQ516_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512323.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            4372..5001
                     /locus_tag="VQ516_001936"
     CDS             4372..5001
                     /locus_tag="VQ516_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2512324.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(5124..7016)
                     /gene="pepO"
                     /locus_tag="VQ516_001937"
     CDS             complement(5124..7016)
                     /gene="pepO"
                     /locus_tag="VQ516_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2512325.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         1985 a         1681 c         1378 g         2176 t
ORIGIN      
        1 ttatcatttt atgacaaaca tactatcgtt aaatgctgat ttaatcgaaa ttttgagtta
       61 taaactcctc agtttcgaac tcaaaattag ccgtgcagga taggataccc ttcataaact
      121 tatataacta ctttaatacc ttaaaaagct aataaaataa ggaatatcta agcaatatac
      181 attctaaaat acctgtacgg gagtgtctct aaagaaaagg aggttttagc atgaaaaaaa
      241 tcatcttcat caaaaccatt caactccttg tcattgatgg aatcatgctg gcatttttga
      301 catttaaaag gggggcttac ttgggactgg attttgattt atagtggttg gctcattttc
      361 tttcatcctg tgctattgac ctatctttca aaccaacttt gtgaccactt tagtcaactc
      421 tattcccaga ttagaccgag attctggcgt tttgctttac aaattctcct atgggatagc
      481 ctgatgattc tctccttggt gtctttaagt gatattccac ttttccttca gggaactctc
      541 ctcatcctag gacatctcat cccttcctat cgcatctgcc aaagcctgaa aagagacttc
      601 ccccaagcat atcaagaacc gatttctttt tggagtattt tatgatcgat gagaaagacc
      661 aagccgactg ggcttggtct ttcttatctc tttttagtat ctaggataat ggtaacaggt
      721 ccgttattaa ccagctcaac ctgcatatct gctccaaaga tacctgtctg aacgggcact
      781 tcttgcgcta atttttgatt gaaagcatca tagaagtctg atgccatatc aggtttagct
      841 gcccctgtaa aggctggacg attgcctttc ttagtatccg caaagagggt aaactgagaa
      901 atagagagga tttctccttc aatatctttg acagacaggt tcatcttgcc ttctgcgtct
      961 gaaaaaatcc gcatattgac cagttttctc acagcatagt ccaaatcttc ctcttggtcc
     1021 tctggtccaa caccaaccag caataaaagt ccctgattga tttttccctg aatctggcct
     1081 tctatactca cttgggcttt tttaacccgt tggataatga ttttcataat agcctttcta
     1141 gtaagagcta ggacaactag ccgttggtcc gtttgacaga gtaaacttct ggcacactct
     1201 taattttatc gacaaccgtg gtcagtgtag agaggttggc aataccgaag gacacatgga
     1261 tattagcaaa cttcatatcc ttggttggtt gggcattgac cgttgaaata ttcttggttg
     1321 tatttgaaag aacttgcagt acatcgttca acagtcctgt acggttgaga ccgtagatat
     1381 cgatatgggc catatactcc ttatttgagc tagagtactg gtcttcccat tccacatcaa
     1441 ggagacgttg ctcgtagttt tcttgggcac gcaggttcat acagtccaca cggtgaatag
     1501 ccacaccacg acccttggta atgtagccaa caatatcgtc accaggcacg gggttacaac
     1561 acttagcaat ccgcactagg agaccagaag caccttcaat aaccactccc ccctcatgct
     1621 tgaccttgag agtttcttta ttttcaacct tgacctcgcc acctttgaca agctcctctg
     1681 cctcagcctt ggccttggca cgctcttcct cacggcgttc cttttcagtc agacggttaa
     1741 agacggtaat cgcaccgatt tccccaaaac caatggccgc aaagagggag tcttctgtct
     1801 tgtaactggt cttttgcaga acttgatcca tgtggcgctt gtccataaat ttatttgcca
     1861 catagccatt ttcttggaac tgagccatca gcatctcacg acccttgttg acagacaatt
     1921 ccttatcttg gtttttaaag aactggcgaa tcttattgcg cgccttgcta gtcttgacca
     1981 tattgagcca gtcacggcta ggtccaaagg agttcgggtt ggcgataatt tcaacctgat
     2041 cccctgtctt taacttggtt gtcagtggaa ccatgcggcc attgaccttg gcaccagttg
     2101 ctttttcacc gaccttggta tggatttcgt aggcaaaatc aatcggtcct gaatctttgg
     2161 gaagagaacg gacagctcca tctggggtaa aaacgtaaat ctcctcagcc agatagtttt
     2221 ccttaacaga gtccacaaat tccttagcat catcagcctg gtcttggagc tccatcatct
     2281 ccttgatcca gttcattcca atagctgatt ccttgctgtt aacttgcccc tttatacctt
     2341 tcttataagc ccagtgagcc gcaaccccgt actcagccac ctcgtgcatt tccttggttc
     2401 gaatctggaa ttcaatcggc ccttttggtc cataaacagt cgtatggata gactgataac
     2461 cattagcctt gcggttggca atatagtctt tgaagcgacc tggcatcggt ttccaaaatt
     2521 catgcacgta accaagcatg gcataaacat cactttgggt atctaaaata caacgaatag
     2581 caatcagatc atagatttcc tcaaaccgtt ttctcttgtc ctgcattttg cggaaaattg
     2641 agtaaatatg cttgggacga ccataaatct tccctttcaa gtgacgttct gtcgtatact
     2701 cctctaattt tgtgactacc tcatccacca aggcctcacg ctccctgcgc ttttccttca
     2761 tcatatgggt aatcttgtaa aactccgttg gattgagata acggaaagac aagtcttcta
     2821 attcccattt gacactggaa atccccaaac gatgggcaag cggggcatag atttccatgg
     2881 tttctttgga aatacgctcc tgcttgtctt ttcgaagatg tttcagggtc cgcatattgt
     2941 gcaagcggtc agacagtttg accaaaataa cgcggatgtc ctcagacatg gccatgagca
     3001 tcttgcgatg attttccgct aattgctcct cgatcgattt gtactcgacc ttgccaagct
     3061 tggtaactcc gtcaacaatc atccgcacat caggaccaaa ctctctttcc aaatcgtcca
     3121 aagtcgcatc tgtatcttcc accacatcat gcaagaatcc acaagctact gttacagcat
     3181 ccagctttag cttagctaaa atacctgcca cttggatagg gtgaatgata taaggctcgc
     3241 ctgatttgcg atattgacca ctgtggcatt caacagcata gaccaaggcc ttatggacaa
     3301 aatgaacatc ctcttccgtt aaatattctt tggttaaagc gacaacttct tcgcctgtta
     3361 aattcacttc tttcggcatc tctactctcc aattcttcct accattttat cactttttta
     3421 agaatatgaa aactagattg gaacagaata agaaaaaaat aattcaaaat tgcttgataa
     3481 ttctgaatta ttggcccata atatactacg aagttagatt ttaaacttag gtgatagaag
     3541 gagagataga agaacggaaa ccatattgta acccaaagac tttctgattt ccccaattcc
     3601 attgaagata cgaaagataa acggtggaac tcgtatcaca tacactggta ccttgactgg
     3661 attttggaat taatactcaa tgaaaatcaa agagcaaact aggaaactag ccacaggttg
     3721 ctcaaagcac tgctttgagg ttgtagataa gactgacaaa gtcagtcaca tatatacggc
     3781 aaggcgacgt tgacgcggtt tgaagagatt tttgaagagt ataaaaatcc tcaagatact
     3841 ttcttctatc ctttagttta taaggagaat acctatgaaa aaactgctat ttctatcttt
     3901 gctctcctaa tgttaggagt ttgctgcctg ttcctattca gccagcaaag ctataaaaaa
     3961 ctcgttcaat actatgctaa cgaccagaac ctgcccagta ggataactta tagtgaatat
     4021 agcgacaaat gagaagccaa ctacggtagc actctaaaca tcacgtctat caaacaagct
     4081 aatgacggag tttatgcaac ctatgaaggg caattgacac ctttccaata ttgataaatt
     4141 gataaccagc ctgtcttcat ctagtcatgc tggtttttaa gttcatttta aatccttacc
     4201 tattctccct aactgtgcta tacttaattt atactcaatg aaaatcaaag agcaaactag
     4261 aaagctagcc gcaggctgtt caaagcactg ctttgaggtt gcagataaag ttgacgcggt
     4321 ttgaagagat tttcgaagag tattagtaca ttctttgaga ttggagttag tatgaaaatc
     4381 cataaaaccg tgaatcctgt tgcctatgaa aatacctatt atctagaagg cgaaaagcac
     4441 ctcatcgtcg tcgatcctgg tagtcattgg gaagccattc gtcagacaat cgagaagatc
     4501 aacaaaccga tctgtgctat tctcttgacc cacgcccatt atgaccatat catgagtctg
     4561 gacttggttc gcgagacgtt tggcaatcct cctgtctata tcgcagagag cgaagccagc
     4621 tggctctaca ctcctgtcga taatctctcc ggtctccctc gccacgatga tatggcagat
     4681 gtggtcacaa aacctgcaga acacaccttt gtctttcacg aagaatacca actagaggaa
     4741 tttcgtttta aggttctacc gaccccaggg cactctatcg gtggtgtttc cctagtcttt
     4801 cctgatgctc acctagtctt gacgggagat gctctattcc gcgaaactat cggacggacc
     4861 gaccttccga ctggtagcat ggagcaactc cttcatagta tccagaccca actcttcacc
     4921 ctaccaaact acgatgtcta tccaggacat ggtccagcta ctactatcgc tcacgaaaag
     4981 gccttcaatc cctttttcta gcaagatgat gacaatcgaa atttaagtaa actatccagc
     5041 aaatctttct attacaaaag gcatcctatc aaggttttca cacgtgattg gatgcctttt
     5101 ttctgatgac tagatttttt gtattaccaa ataatcacgc gctcctctgg tgaacgccac
     5161 attccgtctc cttctttgac atcataggtt gtaaagaaat cgtcgaagtt tggtacttgc
     5221 acattgacac ggagtttggc tggtgcgtgc acatcgacgc tagccaaaag tttcataaat
     5281 tctggtcgac ctttcatgcg ccagatgcga ccgaagttgt agaagaactc ttctgctgag
     5341 aagtctgctt ctctcttagc tgcttcaagc gctgctgcga ttcctcccaa gtcagccacg
     5401 ttttctgata cagtcaattt accgttaatg gttgctccat aagaatcctg tccatcaaat
     5461 tggtcaatga ctttttgtgt tttctccttg aaggcagcat agtcgctctc tgtccaccaa
     5521 tccttgaggc taccattttc gtcaaaggaa gccccgttag tatcaaaggc gtgggaaatt
     5581 tcatgggcaa tcactgcccc aataccaccg tagttagcag aagatgactg atgcaagtca
     5641 tagaaaggcg cctgtaaaat ggccgctgga aagacaatca ggttcttctg aggattgtag
     5701 taggcattga ccatatgagc aggcatgccc cattccttat agtctacagg ctggttccac
     5761 ttactccaac tgtgcttgat ttccacacgc gcaaaggcta gagcattctc aaaaagactg
     5821 gcagtttcat tcactacctt atccttgtaa cgtgcaggca attcttctgg atagccaata
     5881 taaggtttga tcacattgag cttcacgata gcctgtttac aggtttctgg agtgagccag
     5941 tcattcttaa gcagacgctc cttatagaca tcaatcatgg ttgccacttt tttctccaca
     6001 tccgccttgg cttctggaga gaacttctca cgggcgtacc aaagccccag ggcttgcttg
     6061 aaaggttcct gtgctagatg ataagctgct ttgaccttat cttttgcctc tggaactcca
     6121 gaaagggcac ggctgtaggc accagacaaa acacggatat cctctgttaa atagctggtt
     6181 gaaagattga caacactcaa aatcaaggtt gctttaagga gagaccaggc ttcctcactg
     6241 taaaattgct ctgctgcttg ccagaaacgt tcctcgtcta caataacctt gtctggtaat
     6301 tgcccaataa ctgctttgaa gaagtcatcc aaaggtaggg caggcgcgaa tttcttgaaa
     6361 tcttcgtaag aatatggatg atagagttta gcatattctg aactttcttc attagagagc
     6421 accactgccg caactcggcg gtccaattca agtctttttt ctagcaagtc ttcaatttct
     6481 tcatcagaga aatcataagc cttgaggaga tttgcgctgc tttctttcca aagagtcaag
     6541 agctcttcgc gctgaggatg ttcttctgca tagtaggtcg tatctggcaa gattgtgctt
     6601 ggagcgctag cccatagaac attgattcta gcatccataa agtctggcga tacaccaaaa
     6661 ggaaggaagt ttggttttcc tgcaagctca aactctgcta gtttagctgt aaaatccgca
     6721 aaagtttcca attcttggaa ttctttaagg agtggtaaga caggtgtgat accgtcagct
     6781 tctctcttgt caaaatcacg aactaggcgg tggtatttga caaagttttc caagatagca
     6841 tcctcaggca cttcttcacc tgctaaccac ttgtctgttg tcgccagcat caggtcttca
     6901 atttcctggt ctaaatcaac aaaacctcct gtttgagact tatctgctgg gatttcagct
     6961 gtctgttgcc attctccatt gatagcatca taaaaatcat cttgataacg tgtcatcttg
     7021 ttctcgcttt catttgtatt tgcatttatc ttaacaaaaa tcactaggga atgcaattaa
     7081 taaccattta actattattt tttagctatt ataaaaaacc tttccatgat aaaacgcata
     7141 attccaagtt ttttacactt gatactatgc gttttataat ttagaaattt atcctgaaag
     7201 ttatctttag aatctatttt
//
LOCUS       DAVBCV010000079         7162 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBCV010000079 DAVBCV010000000
VERSION     DAVBCV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.079"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(741..1001)
                     /locus_tag="VQ516_001938"
     CDS             complement(741..1001)
                     /locus_tag="VQ516_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512326.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(1060..1611)
                     /locus_tag="VQ516_001939"
     CDS             complement(1060..1611)
                     /locus_tag="VQ516_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512327.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1601..1798)
                     /locus_tag="VQ516_001940"
     CDS             complement(1601..1798)
                     /locus_tag="VQ516_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512328.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(1802..2386)
                     /locus_tag="VQ516_001941"
     CDS             complement(1802..2386)
                     /locus_tag="VQ516_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512329.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(2794..3219)
                     /locus_tag="VQ516_001942"
     CDS             complement(2794..3219)
                     /locus_tag="VQ516_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512330.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(3212..3706)
                     /locus_tag="VQ516_001943"
     CDS             complement(3212..3706)
                     /locus_tag="VQ516_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512331.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(3752..3958)
                     /locus_tag="VQ516_001944"
     CDS             complement(3752..3958)
                     /locus_tag="VQ516_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512332.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(3945..4106)
                     /locus_tag="VQ516_001945"
     CDS             complement(3945..4106)
                     /locus_tag="VQ516_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2512333.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(4471..5427)
                     /gene="lytA"
                     /locus_tag="VQ516_001946"
     CDS             complement(4471..5427)
                     /gene="lytA"
                     /locus_tag="VQ516_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2512334.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPEGLITVK"
     gene            complement(5803..7155)
                     /locus_tag="VQ516_001947"
     CDS             complement(5803..7155)
                     /locus_tag="VQ516_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2512335.1"
                     /translation="MDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVGRFLGQE
                     SLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWILGALLSI
                     LVMLLGFLGLYHLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLRSIGDSL
                     AALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRKSVPELL
                     PQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQTAARRI
                     MTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIFLFFASP
                     ALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPLVSSFIE
                     LIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAILATKVQS
                     "
BASE COUNT         2298 a         1486 c         1132 g         2246 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga tcagcatttc ctagccaata ggacgctcca aatcacctat tctaagtcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtggt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac tagaatagta catctctgct tctaaaacat tgttagaaat cgatttgact
      481 gtcctgatca atttgtcctg ttctcgtttc atttcactat actaaaaaac cacctgaaac
      541 aaatcaagtg actagaggat aaaaacaaag actaggcaaa aagttttgaa aagcaaaaaa
      601 cgcataatat caggtcttga aaaaccttga tactatacgt ttgattgtgg gaagattgac
      661 ttcattttct cctgaaattg aagttttttc tagtttccag atatagacga attcaatata
      721 gccatctcgc tacaattatt ttaaccatcg agaaaatttt tcagaatttc aaaaatttca
      781 ttttgatcca atgaaatgtc atcattttga agaatagttg acttcacagg ctctaattct
      841 atcacaaagc ggtgtatgtc tgcttttacc gcactacttg atgttatagc cgataacttt
      901 tcatctccta gcaaggaagc tgtcaaacgg gtaaggtcat tcaaatgctt tttaatggac
      961 ttacttaaac cttgagcacc tgtagcagag cgttccctca tatccagcca agctttagcc
     1021 ttaaaaataa ttaatactca atgaaaatca aagagcaaac taggaagcta gctgcaggct
     1081 gctcaaaaca ctgttttgag gttgtagatg gaagctgacg tggtttgaag agattttcga
     1141 agagtataaa ccacaattac tcaataccga atacccctga atggtttctt tttcatgcac
     1201 caatatatta taataatctt catccaataa taaggctgat aaactagcat cttggtcaaa
     1261 atgtaaggga atttctcgac cgtccttctt taatggatat tctggtaaga tactaaatag
     1321 ttcaatcata gaaggaaact caggattagt tgttgtaaat cgaaaaagct gcgctttctc
     1381 atctttctga cttccttgat actctcccaa ttctaaaaaa tgattcaagg tagtataaaa
     1441 ttccttattt tttacttcat aaatgatgac catatcataa tcttttgttg tgcgactttt
     1501 aaatccttgc gaatccaata cgatagaggt agcagttccc ccaatcagaa cataatagtt
     1561 ctgaaaatcc gcaaacattt cttgaaaaat aactttcgta ttagctggca tcatcttctc
     1621 ccagatactg taatatcata ttttctagtg cttcactctc ttcctctata cgtgggtcat
     1681 catcatcttt taaggtcaaa taaagagaaa tcggatctac aaattgttta tcatgattat
     1741 ttttaaaatc attccaaaac tcagatacaa aaggacgata tttccatatc tctagcatct
     1801 ttcattttaa aacatgctga gaaagtggca aggataactg attgaatttt ctctgccata
     1861 tgacatagct agtattttca tccgtttcag ctaaaaaagt tgaatgcgac aaagcataag
     1921 caccaccata tagaaggtta gaaacagatt ttatctgctt tatatcgcca tctggcaata
     1981 aaaaggcaat ccacgttaat tgttcgctag gtgttaattc cttagggatt tcagtatcat
     2041 tcgcattgag tactagtccc aatggaggga agaagaggtt tcccttaaag tctacaaacg
     2101 gaactctagc ttgaagtaat tgcttttttt cactgtctga taacttcgaa aacaccaaaa
     2161 caacatccat attggctttt tcacccatag tgcgagcctg agtaacaaat gaactcaaac
     2221 tcccccttct cttttcttta atcaataaaa atgattcctt atgaaaagtc atttgagtat
     2281 actcaaacct ttttacaaaa ctgattggta aagtcaaagt cagttcctca atactgacat
     2341 caacataaaa ggattgaaaa attgacatta tctttttgac attcatgttt tttccttatt
     2401 ctatcatttt tattatattt tatcattcgg tataataaaa tataagtttt ttgataaaat
     2461 aaccaaaaac tactcgctat cccctctcac attaccctac atatcgtttg acatgcgact
     2521 gatagttcag gaaaacttca aggagaactt ttctctcatc cactatgcag gacttactat
     2581 ttcacttcta ctcctatagg ctcaatttga cacttttctt tttcgaattg ctcatatgcc
     2641 tcctgaatag tgagttcgat ttatccaaat ttatttttgt ctattttgat gaatacttcc
     2701 acgaagatag tctgaaaatg aatttgcacc aattttcctt ctctttttaa gcatattatt
     2761 ccagatatga ttgccctctt gttcagtaat aaattacgct ttaaacgctt caatcagtat
     2821 atctcctgtt gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa catctcttag
     2881 gttattactt cctaatatcc cattatgctt tttcgctaag aaaatagaag tcgcttctcc
     2941 cttaccaata atcttgttac tatcataatt tcttgttaaa tctctatata atgcgtattc
     3001 ttcagttcca atgtctatgc tcacaatctc agctgaaccc ttagttacca actggtctat
     3061 cctagatttt aaatggggaa ttgtaggtat attgatttca tcatacacct cttgtggaat
     3121 aacaatttta cccgaataga gcttttctaa aagatgttca gtgccaaccc ataaaaatgc
     3181 tgaaatacaa tctgcatcaa taaatacacg actagtcaaa cgacaactcc ccctcttcat
     3241 ctagcccata tacaatatca tatctgaaag catctagtaa cagttcctca tacttccctt
     3301 gcgaaattct gttattttct aaaagttgtt cagtcgattt aatatagtgc cctaatacca
     3361 tttccttttt actttctgac aaaggacgat ataaacttgt atcatagcct aatcttgaag
     3421 ctgtctctat aacactaata tccatatttt taatttcttc tgcatcaagg tatccatcat
     3481 ttctcaatct atataacata gctttatgac tgataccata aaactgaccc aattttataa
     3541 tatcttctac ttcaagatga gttctattgg cattttctct gatttcctca accatcctat
     3601 acagtgaaga tgggaaaatt aaaaaataag aagcaaactg atccgctttt ctttcagttt
     3661 catctccttc accaatcaag ataagactga ctgaactctt cttcacctca tcataataaa
     3721 gatgatacag tccatgtgct aaagaaaatc tttaccttcc taatggcatg tctgaattga
     3781 ctgcaatgag actgaactga gttcctttat aacagactcc gctaatattc tttccgagtc
     3841 cataaaatac cagcgtcaaa ttttctatct tttgtaccaa tttaaaaata tctatcggcg
     3901 attcaccatc agctcccaac ttttttctaa gatttgaagc tttattactt aaatccattc
     3961 gtgatattcc tttcaccttt ttccattttg gcaatcatgc tttgattcaa agacaaatac
     4021 tcagcaatct tatcttgagt aagatttgaa gatattctaa gttcttttat tctttttcca
     4081 acatcacata catttatcat attcatacac ctctcataaa aagaatagca cattcttgtc
     4141 ataatttttt aaataaaaaa attatgacaa aacaaggaag caatttattg atgctactta
     4201 aaaatctaaa attgatgata ttaaacctat ttgatgagtt cctatcaaaa atcgtatctt
     4261 caacctcaaa acagtactta aagctatccg actcggttta cattgtcaaa tttagatttt
     4321 atttgagcat aactttctag tttgcttttt gatttttgtt taatatagta acaaaaattg
     4381 aaggaaatat ctccacaaga aaacgcatac tattaagctt tttcaagacc taataatatg
     4441 cgctgttctg atttgaaaga cattccatta ttattttact gtaatcaagc cttcaggctc
     4501 tactgtgaag tctggcttgt ctgccagtgt tccgtctggt ttgaggtagt accagcctgt
     4561 tccgtccgct gactggataa aggcatttga taccatggcg ccttctttag cgtctaagta
     4621 gtaccaagtg tccttgtact tgacccagcc tgtcttcatg gcaccttctt cgttgaaata
     4681 gtaccactta tcagcgattt tcttccagcc tgtagccatt tcgcctgagt tgtcgaacca
     4741 gtaccagttg ccgtctgtgt gcttcctcca gcggtctgca agcatatagc ctgaactgtc
     4801 aaagtagtac caagtgccat tgattttctc aaacttgtct tttggataag agccgtctga
     4861 atgtacgtac cagtagccag tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt
     4921 ctcaatatca tgcttaaact gctcacggct aatgccccat ttagcaagat atggataagg
     4981 gtcaacgtgg tctgagtggt tgtttggttg gttattcgtg caatactcgt gcgttttaat
     5041 tccagctaaa ctccctgtat caagcgtttt cggcaaacct gcttcatctg ctagattgcg
     5101 taagagttcg atataaaggc ggtagtccgt catgaactct tctttggttg aatggctttc
     5161 aatcagttca accgctgcat aggtctcagc attccaaccg cccccaacgt cccaggcacc
     5221 attatcaaca ggtcctacct gcatgatgca accgttccca acaatgtgcg agaaaaaacc
     5281 taattctggg tctttccgcc agtgataatc cgcttcattc tgtacggttg aatgcggatt
     5341 cccagttgag tgtgcgtgta cttgcctata tggttgcacg ccgacttgag gcaaatctgt
     5401 tcttaattta ctcacattaa tttccatatt ctactcctta tcaattaaaa caactcattt
     5461 tttacaatcc agaaccagaa actcctttat ttctacctta caaagaagac aatcttagtc
     5521 acgattaagc ttgtagatag aacctcaaaa cgcactattt tgacactgta aataggactg
     5581 acaaggtctg cattctatct acaataacac cccagactaa aaagcttttc aaaagtatat
     5641 ttttacagtc tctatatgtc cttttcataa atactatact ttattatatc acataaaaga
     5701 agtcaaaagt ctgttaaact attttcaaca ccaaactaaa gaagagaaca caagtttttt
     5761 cgatgttcac tagaggaaat ggattttatt cagtaaatcc aactaggatt gcactttggt
     5821 tgccaaaatt gcctttcctt cttttatcaa gggatgacgg aatagtgaga agtacagttg
     5881 aactgtcatg gcaacccaga taagaggttc acaaaggata acacccttat atcctgccca
     5941 aggaataatc aaaaccacaa aaacgatttt tccgattagt tcaataaagc tagaaactag
     6001 aggaaggatc ttctgcccca agccctgcaa gcaattgcga taaatcaaca agaggctcaa
     6061 aatgggataa aaggctgaac tgatttgcag atagagactt ccattttcta tcaagtaacc
     6121 atctgtcgaa ctagccaaga aggaaaccaa ggctggactg gcaaaaaaga ggaaaataca
     6181 aacaaaaact gcccaggata tacttaaacg actgccgatt cgaagacctt gaacaatgcg
     6241 gtcaggtcgc ttagctccta gattctgaga agcaaaggtc gtcattgatg cagaaatagc
     6301 ggtcatagga agaagggcaa aggtcataat gcgtcgagct gccgtctggg cactaataat
     6361 cactgcacca aatgtattaa cagaaaactg taaaatcaca ctgccgatag atacaattga
     6421 actcatcaag cccatagcca aaccttgctc caagagatcc gcgtacaagc ttttgtccca
     6481 tttgaaatgt ttaaactgtg gcaagagttc tggcacactt ttacgaatat aataaaagca
     6541 gagaaccgct gataaacctt gcgaaataat ggtagcaagt cctgcggatt gaactcccag
     6601 atgcaattgc gtaataaaat agagatccag aaccacatta accaaggcag agaaaatcag
     6661 aaatcccagg gctgctagac tgtcaccaat agaccgcaac aagcctgcaa aaagattata
     6721 agcaaagctg acacctacac aggtcacaat catagaaata tattgataag attgaggaag
     6781 aatttctgca ggagtatcta agtattgcaa gagatgatac aagccaagaa agcccagcaa
     6841 cataactaga atgctcaaaa gagcacctaa aatccaagta gctgctactg cttccttgat
     6901 tttagtgaaa ttccgagccc cataataacg agcaatgaca atccccatgc cattgccaac
     6961 accaagtgta aaacctacaa tcaggtcaaa aatcgctgtc gtcgctccta ctgcagccaa
     7021 ggattcttga ccaagaaatc gtccaacaat caagacatca gcagtgttat agagctgttg
     7081 aaaaatattt gatagcaaaa ttggaaaggt gaagcttaag agcgagggaa gaatcggacc
     7141 atgtatcagg tccactgttc gt
//
LOCUS       DAVBCV010000080         7087 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBCV010000080 DAVBCV010000000
VERSION     DAVBCV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.080"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(147..2144)
                     /gene="metG"
                     /locus_tag="VQ516_001948"
     CDS             complement(147..2144)
                     /gene="metG"
                     /locus_tag="VQ516_001948"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2512336.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEYDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2268..3527)
                     /locus_tag="VQ516_001949"
     CDS             complement(2268..3527)
                     /locus_tag="VQ516_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2512337.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKKSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3529..4230)
                     /locus_tag="VQ516_001950"
     CDS             complement(3529..4230)
                     /locus_tag="VQ516_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2512338.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4214..5413)
                     /locus_tag="VQ516_001951"
     CDS             complement(4214..5413)
                     /locus_tag="VQ516_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2512339.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILIPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5699..7045
                     /gene="gor"
                     /locus_tag="VQ516_001952"
     CDS             5699..7045
                     /gene="gor"
                     /locus_tag="VQ516_001952"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2512340.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         2048 a         1572 c         1341 g         2126 t
ORIGIN      
        1 catgttttga gctgacttcg tcagttctat ctacaacctc aaaacagtgt tttgagcaac
       61 ctgcggctag cttcctagtt tgctctttga ttttcattga gtataaataa aaaaacagaa
      121 ctagtctgaa ctagtcctgt ctacttttac ccaatcacac ttccatttgg tacagctgga
      181 tcaactgtga gaagggttaa tttgccatca tattcagctg atagaatcat accctggctg
      241 acatattttt tcatcatttt acgtggtttg aggttagcaa cgatttgaac tttcttgccg
      301 accaattctt gttcatttgg atagtatttt gcaattcctg aaagaatctg acgatcttct
      361 ccatcaccag catccaagcg gaattgaagc aacttatctg aaccttctac tttagacact
      421 tctttgactt ctgcgacacg gatttcaacc ttgtcaaagt cttcaaactt gatttcatcc
      481 ttgtttagtt tgagctcaac ttcgtccgga ttccattctt tttcgactgc tggtttattg
      541 ccttccattt gttccttgat ataggcgatt tcttcttcca tatttagacg tggaaagata
      601 ggtgttcctt tggcaactac agtcacatct gctgggaagt cagccaaact caagttttca
      661 agactagaaa cttcttccaa accaagttga gtcaaaactg cacgactagt ttccatcata
      721 aatggttcaa tcaagtgagc aactacacga atgctggctg ccaagtggct catgacactt
      781 gccaattggt cacgaagagc ttcatccttg gccaagaccc atggtgcggt ctcatcgatg
      841 tatttattgg tacgagagat cagagtccag actgcttcaa gcgcacgtgg atagtcaact
      901 gcttccatgt gtgtatggaa gtctgcgatt gattgttctg caacctcagc aagaacatga
      961 tcaaattcag tcacaccttc tacataggca gggatttgtc catcaaagta cttattaatc
     1021 atggaaaccg tacggttaag gaggttccca aggtcattag ccaattcata gttgatacgg
     1081 ccgacatagt cttcaggagt aaaggttccg tctgaaccaa ctggaaggtt acgcatgagg
     1141 tagtaacgaa gtggatctag tccataacgc tctaccaaca tttcagggta aacgacattc
     1201 ccttttgact tagacatttt tccgtctttc atgacaaacc aaccatgggc aatcaaacga
     1261 tcaggtaatt taacatccaa catcataaga aggattggcc agtagataga gtggaagcga
     1321 aggatatctt ttcctaccat atggaagact gttccattcc agaacttgtc aaagttacca
     1381 tgttcgtctt gagcgtagcc aagagctgtc gcatagttaa gaagggcatc aatccaaacg
     1441 tagacaacgt gttttggatt tgatgggaca ggcactcccc atgtaaaggt tgtacgagat
     1501 accgccaaat cttccaagcc tggctcgatg aagttgcgta gcatttcatt aaggcgacca
     1561 tctggcgtga taaattcagg atgagctttg aaaaattcga ccaaacggtc ttggtatttg
     1621 ctaaggcgaa ggaagtatga ttcttcagaa acccattcaa cctcatgacc tgatggagca
     1681 ataccaccag tcacatttcc agcttcatca cggaaaactt ctgccagctg gctttctgta
     1741 aagaattctt cgtctgatac tgaataccaa ccagagtatt cacccaagta gatatcatct
     1801 tgagcaagta agcgttcaaa gacctgtgcg acaacttttt catggtagtc atcggttgta
     1861 cggataaatt tatcgtatga gatatctagt aattgccaga gttctttaac tccaaccgcc
     1921 attccatcaa cataggcttg aggtgtaata ccagcttctt ccgctttctg ctggattttc
     1981 tgaccatgtt catcaagacc tgtcagataa aatacatcgt agcccatcag gcgtttgtaa
     2041 cgtgctagga catcacatgc gatagttgtg taggcagaac cgatatgaag tttcccagat
     2101 ggatagtaaa tcggcgttgt aatataaaaa tttttttcag acataatttt tcctttccag
     2161 gcaaatgaaa cctgtttttc taacacttca ttatatcaca tttttaatga atttcgatag
     2221 ggaaatccat acaaaaacaa gatagacgag tgtccatctt gttgatctca ttcataacga
     2281 agggcttcaa ttggatcaag tttcgatgcc ttgttggctg gcaagactcc aaaaatcata
     2341 ccaacactag ccgaaactgc aagactaaat agggcaactg ggattgatac tccaacttct
     2401 ataccttcta ttaaaccttg cagtaacaaa cctgctaagg cagttaaacc acttgcaatt
     2461 gtcaagccaa ttaagccacc taacaaggtc aaaatcatgg attcaatcaa aaactgaatt
     2521 aaaatattgg cacgtgttgc acccaaagct ttacgaagac caatctcacg agtgcgctct
     2581 gtcaccgaaa ccagcatgat gttcatgaca ccagttcctc caacaaagag agaaatccct
     2641 gcgatggaac taataatcgt cgtcataaaa ctaaacgatt gttgaatttc tgcaaataca
     2701 acggactcat ctgccacctg gtattctccc tgttgtaagc ctgcaagctc tgtcattttt
     2761 cgtgccagtt ctggacccag agttggggtt aaactggtat cattcactcg aaagacaata
     2821 ctagctattt catctatatt aaaattcgca gcaagggaga tattggtagt aataggcaag
     2881 ccaccaaacc catatatttt tgacttttta gcctccggac tagtataaac cccaatgacc
     2941 cggtaactaa atccattgac ttctacaacc ttgttaatag cctcttgagg agattcaaat
     3001 aaactaatgg acaattcctc atctagcaaa ataacacttg caaactcttt gaaatcttgc
     3061 tctctcagac tacgacctgc aataatttca ttcttaacag cgtccatgta agttctgttt
     3121 ccacctgtca aattagcatt ctcaaccttt ttatcttgat aggtcaagat ggcattcgtt
     3181 gaattggtta catagtaact atccactccc ttcagtttag ctgcctcttg aacccaggat
     3241 tcttgcggtt ttggcggttc aacaggaact tcctcttcct ttccagaaac cgtaaaagct
     3301 gattgtttct gagtaaaaga cccatcttta ctttttttag gagagaaaaa gacgctaata
     3361 tttttctgag atttagtcat atctttattg acttgacgag atagggaatc acccaaagcc
     3421 ataatcacaa caactgatga aacaccgata ataatcccaa tcatagtaag caaagaacgc
     3481 atcttgtgag ccatgataga tgaaaaggca aatttcagat tctgcatctt agttttcctc
     3541 ctttcctaac tgagcactgt cagacgaaat gaccccatcc cgaatgacaa tctgacgttt
     3601 ggcataggca gcaatctcag gctcatgcgt taccatgata atggtttttc cttctttatt
     3661 caaatcaacc aataattgca taatttggtt acctgttttg gtatccaagg ctcctgtcgg
     3721 ttcatccgct aggataatag aaggattgtt taccaaggca cgcgcaatgg ctacacgttg
     3781 cttttgacca ccagataatt ctgaaggtaa atggtgacta cgttctgtca attcaacctt
     3841 gtctaaatat tcctcagcca acttgcgacg ttttgaagac gaaactcctg cgtaaatcaa
     3901 gggcaattct acattttgca gagcattgag cttcgataga agaaagaact gctgaaagac
     3961 aaaaccgatt tgttggttac ggaccttagc tagttgtttt tcaccaagcc cagccacttc
     4021 ttgaccttca agataatatt ctccactggt tggtgtatcc aacatgccaa tcgtattcat
     4081 cagagtggac ttaccagacc cagatggtcc catgatggct acaaattcac cctcattcac
     4141 ttctagattg atatttttga gaacctgcag ttcttggtca ccattacggt aacttctgaa
     4201 gatatttttt agactaatta gttgcttcat cagccttcac ctcttttcct tcttccaagg
     4261 aagatgttgg attactgatg accttagcac cgttcgttaa accagaagtg atttcttgat
     4321 tttctgcgtc agcatttccc aatgaaacct caactttttt agccttttgt tgttcatcca
     4381 caatccagac ataattttta ctatcatcca ttactagact gctaacagga ataagaatag
     4441 ccttagtttt gcttttaacc tcaatgttga cagaaaaacc ttgtttcaaa tcaccaacct
     4501 cgcctgtcac atcaatagta taagggtatt tagaacctgt attattcccg gctgctggac
     4561 tagctgcttc accattgttt ttaggatagt cagaaatata gcttaatttc ccagtccatt
     4621 ttttatcagg atacacttta gaagtaaagc ttacttcttg acctacagaa aggttggcta
     4681 gattgtactc agacaattct cccttgactt gtaaattttc attgctgaca atatgaacca
     4741 taacttgact cgcccctgtt ggagatttag aaacattgct attgacttcg actacagttc
     4801 cctctagggt actgagaaca gttgttgcat ccaattgact ttgagccttg cttaattgcg
     4861 ctgcagcatc tgcacgcgca tcacgggcat cacccaattg agcatcaata gaagcaacag
     4921 aatttccagc cactggagtt gggctttgca ccgttgcatc ttctcctcct actggcgctg
     4981 gtaactgtgg agcctgagct gaagcggctt catttcgtgc ttgattgagt tcattgatat
     5041 gacgatctgc cttagctact gctcgactag ctgaatcata ggccgcctgc gcttctgaac
     5101 tactgtactt gactaaagcc tgcccttcgc tgaccttatc gcccacagaa acaaggattt
     5161 catctaaatc acccttacta gcatcaaaat aaacatattg ttcatttttt gctgttactg
     5221 tccctgacaa taaaacagag gatgccacgc ttccttcctt ggcaacaaca agatgagtag
     5281 gctcatcttt tacagcagtc tgagaaggtt gtctaaaaag taaaatcccc ccggcaccca
     5341 atacaactac actcgcagca ccgattgctg catacagttg ccacttttta gctttaccat
     5401 tctttttctt cataatgaaa ctccttttct tttttacaat actttgctat tataccaaat
     5461 ttccctccag caaacaatgc agttcagatg gaaataatgg cagttcagga ttaaacaatc
     5521 gttcggaatt ttgcttttct attgtactag atatataata cactatcttg acttatttgg
     5581 caagcttttt tgcaattttt tgtgcgtagc agatttttgc aatcaaatca aaaaaagaat
     5641 aaaatataac tatcaaaaaa gacactctac tatttaaaag attacaaagg agttttcaat
     5701 gagagaatat gatatcattg ctatcggtgg aggtagcgga ggaatcgcta ccatgaaccg
     5761 tgctggtgaa catggagcca aagcagccgt tattgaggaa aagaaattag gtggaacctg
     5821 tgtcaacgtc ggttgtgttc ctaaaaaaat catgtggtac ggggcgcaaa tcgctgagac
     5881 tttccatcaa tttggagaag actacggctt taagactact gatcttaact ttgactttgc
     5941 aaccctacgt cgcaatcgtg aagcctacat tgatcgcgct cgttcttctt atgatggtag
     6001 ttttaaacgc aacggtgtag acttgattga aggtcatgct gaatttgtag attctcatac
     6061 tgtaagcgta aatggtgaac tgattcgtgc taaacatatc gtgattgcta caggtgccca
     6121 tccaagtatt cctaatattc ctggtgctga gctaggtggc tcttctgatg atgtatttgc
     6181 ctgggaagaa cttccagagt caattgccat tctaggcgct ggttatatcg ccgttgaatt
     6241 agctggcgta ctccacactt ttggtgtcaa gacagatctc tttgttcgcc gcgatcgtcc
     6301 tttacgtggt tttgattcct acatcgttga aggtttggtc aaggaaatgg aaagaacaaa
     6361 cttaccactt cacactcaca aagtccctgt caagttagaa aaaactactg acggcattac
     6421 cattcatttc gaagatggta ctagtcacac agctagccaa gttatctggg ctacaggtcg
     6481 ccgtccaaac gttaagggct tgcaacttga aaaagctgga gtgactctga acgaacgtgg
     6541 ctttatccaa gtggatgaat accaaaatac tgttgttgag ggaatctatg ctctaggtga
     6601 tgtaacgggc gagaaagaac tgactccagt tgcaatcaag gccggacgta ccctatctga
     6661 acgtctcttt aacggaaaaa ctactgcaaa aatggattac tcaactattc caactgttgt
     6721 cttttcacac cctgctatcg gaactgttgg tttgacagaa gagcaagcta ttaaagaata
     6781 cggtcaagac caaatcaagg tttataaatc aagctttgca tctatgtact ctgcttgcac
     6841 tcgcaaccgt caagaatccc gtttcaaact cataacagct ggttcagaag aaaaagttgt
     6901 cggacttcat ggaattggct acggcgttga tgaaatgatt cagggatttg ccgttgctat
     6961 caaaatggga gcaaccaagg ctgactttga tgcaactgtg gcaattcacc caactgcatc
     7021 tgaagaattt gtaaccatgc gttaatcgaa acaaaagaag ctgatataaa tatgtctgct
     7081 tcttttt
//
LOCUS       DAVBCV010000081         6989 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBCV010000081 DAVBCV010000000
VERSION     DAVBCV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.081"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            226..6657
                     /locus_tag="VQ516_001953"
     CDS             226..6657
                     /locus_tag="VQ516_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2512341.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VVDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPYGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANHADEKG
                     MISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGE
                     ASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAY
                     DDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
BASE COUNT         2759 a          943 c         1348 g         1939 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 gtagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcacaggatt ttaaagagaa gaaaacagca gtcattgagg gaaaagaagt tgttagtaaa
      481 aatcctgtga cagacaataa cactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagatcatac ggactcattt gtgaataaaa acacagaaaa tcccaaaaaa
      601 gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgaag atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccatatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgcg gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctatttgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcat gctgatgaaa aggggatgat ttcttattac
     4201 ctagtagatc ctgacaaaga tgcttctaaa tatgaaaaac ttggtgaaat ttctgaagat
     4261 aaactcaaaa atgcaaagag cccagaggaa aataccaata ataatcaagc taaggatgaa
     4321 gattcaaaac cagatgaaaa aagttcagtt gagggagaag ctagccttga aataaataaa
     4381 actatttcaa caattagaga gtttgaaaat aaagacctaa agaaactcat taaaaagaaa
     4441 tttagagaag ttgatgattt tacaagtgaa actggtaaga gaatagagga atacgattat
     4501 aaatacgatg ataagggaaa tattattgct tatgacgatg gtagtgcctt acaatatgaa
     4561 actgaaaaac ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4621 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4681 ggaaatagct ataaatcgat agaaatcaaa gcgactaagt atgattcaca ttcaaaaacg
     4741 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4801 gatatgagat tatttgttaa agatgacaat caaataaaag ctgaaactaa gattagaatg
     4861 cctgaaaaaa ataaggaaac taaagcagaa tatccctatg tatcaagtta tgggaatgta
     4921 atcgaattag gagaaggaga tctttcaaag aacaaaccag acaatttaac taaaatggaa
     4981 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5041 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5101 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5161 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5221 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5281 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5341 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5401 tccaatgtag aagctataaa agaagatggc tccatgttat ttattgatac caaaccagta
     5461 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5521 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5581 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5641 actagagatt ttaatattaa gctgaatgtt aaagacggtg acatcatgga ctggggaatg
     5701 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5761 cttcaaactg gctatagcga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5821 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     5881 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     5941 gagattcaag aacaacatat ttatgtaaat ggaaaagaat atacatcatt tgatgatatt
     6001 aaacaaataa cagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6061 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtaagtga attaaaacct
     6121 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6181 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6241 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6301 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6361 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaat
     6421 gaaagtcaca gaaaagagga tttacaaaga gaagatcatt cacaaaaatc tgattcaact
     6481 aaggatgtta cagctacagt tcttgataaa aacaatatca gtagtaaatc aactactaac
     6541 aatcctaata agttgccaaa aactggaaca gcaagcggag cccagacact attagctgcc
     6601 ggaataatgt ttatagtagg aatttttctt ggattgaaga aaaaaaatca agattaagac
     6661 aaaagctata gaaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt
     6721 tttgtgaaaa cagccattta taactcaatt atttagttta cttcactatt gatactattt
     6781 ggagttatta atggacttag tttagataac taatgaattg attgaaaggg ttagtattga
     6841 caatattggt catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt
     6901 gatgagacaa tttcggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
     6961 aggacaaatt ttgtcctttc ttttttgat
//
LOCUS       DAVBCV010000082         6889 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBCV010000082 DAVBCV010000000
VERSION     DAVBCV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.082"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..316
                     /locus_tag="VQ516_001954"
     CDS             <1..316
                     /locus_tag="VQ516_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512342.1"
                     /translation="ELIAPMTYEETMTSDFFEVWFQKFFLPTLTTPSVIIMDNVRFHR
                     MGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWVHIKKHLKKVLPSCNTFYEALLSCSC
                     FN"
     gene            377..1339
                     /locus_tag="VQ516_001955"
     CDS             377..1339
                     /locus_tag="VQ516_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2512343.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1516..2523
                     /gene="gap"
                     /locus_tag="VQ516_001956"
     CDS             1516..2523
                     /gene="gap"
                     /locus_tag="VQ516_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2512344.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2590..3426)
                     /locus_tag="VQ516_001957"
     CDS             complement(2590..3426)
                     /locus_tag="VQ516_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2512345.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3552..5747
                     /locus_tag="VQ516_001958"
     CDS             3552..5747
                     /locus_tag="VQ516_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2512346.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVAAVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIAYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5800..5952
                     /gene="rpmG"
                     /locus_tag="VQ516_001959"
     CDS             5800..5952
                     /gene="rpmG"
                     /locus_tag="VQ516_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2512347.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5962..6138
                     /gene="secE"
                     /locus_tag="VQ516_001960"
     CDS             5962..6138
                     /gene="secE"
                     /locus_tag="VQ516_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2512348.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6194..6730
                     /gene="nusG"
                     /locus_tag="VQ516_001961"
     CDS             6194..6730
                     /gene="nusG"
                     /locus_tag="VQ516_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2512349.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2143 a         1198 c         1495 g         2053 t
ORIGIN      
        1 tgaattaatc gctccaatga cttacgaaga gacgatgacg agcgactttt ttgaagtatg
       61 gtttcagaag tttttcttac caacattaac cacaccatcg gttattatta tggataatgt
      121 aagattccat agaatgggga agctagaact cttgtgtgaa gagtttgggc ataaactttt
      181 acctcttcct ccctactcac ctgagtacaa tcctattgag aaaacatggg ttcatatcaa
      241 aaagcacctc aaaaaggtat taccaagttg caataccttt tacgaggctc ttttgtcttg
      301 ttcttgtttc aattgactat atatcacgga tacagttggg aaagaagcta ttaaacattt
      361 ggagagtcct tttgggatga acatgtcaaa tattactata gatgggcaag aaatggatct
      421 gagtcaaacg atgccttctt atgctcaagt agtgagtctt tatgatgata caagtatctc
      481 tgttagtagt aatgagacgg acaaagtgtt ggctggctcc ctctatactg atacaaatga
      541 acagggatta acgattccaa gcagtttact aaaaaattgg aatgaacaga caggaaaaaa
      601 tttgacagct aatgatctta ttggcaaatc agtctcagcc agcattgtag aaagtgctgc
      661 cgaaactagt aagattgctc aatttcaaac gaagattgta cgtgtaatca atgatgaaga
      721 tgacatggag gacagcaaca gtttcatgct gtctcatcaa atggaaacga ttttgaaaga
      781 ggctggattt acgaaagctg tatcttattt tatcttggaa ctcaaagatc catcacagac
      841 aaaagtagta acagaagaat tacagaaaaa taagaagtat actgtgcttt ctcaacagag
      901 agttcttgat attgtgatta tctttattcg tgttattcag ggattattga ttgtgctttc
      961 atcacaagct attgtggtag cagcggttat gattggtatc attatttaca tcaatatcat
     1021 gcagcgttcc aaggaaatag gtgtcatgaa ggcagttggt tatcagaatc gtgatgtcaa
     1081 aggaattttt atttacgagg ctatctggat tgtaggcatc gccttgctgc tggcattttt
     1141 ggttgcacaa ggggtgggaa gtttggcgaa tgcgattgta agtcactttt acccatccat
     1201 cactaaagtt tttgaattaa atcttttatc tgttttagga actctagttt tcgctctatt
     1261 acttggttat gtctcagcct acttcccggc gcgtaagatt agtaaaatgg atcctgtaga
     1321 accgttacgc tatgaatagt aagggattcc ttgatttaca gtcttaaaaa gaaactaaca
     1381 tagaaaaaca atggacatac ttgcaattca actcaaatag ttgtataata ggtttgttga
     1441 aaggtgattt gtagtgattc aagttactct tttcacaata aaaatttcat gattttcata
     1501 aggaggaaat cactaatggt agttaaagtt ggtattaacg gtttcggacg tatcggtcgt
     1561 cttgctttcc gtcgtatcca aaacgtagaa ggtgttgaag ttacacgcat caacgacctt
     1621 acagatccag ttatgcttgc acacttgttg aaatacgaca caactcaagg tcgtttcgac
     1681 ggtactgttg aagttaaaga aggtggattt gaagttaacg gtaaattcat caaagtttct
     1741 gctgaacgtg atccagaaca aatcgactgg gctactgacg gtgtagaaat cgttcttgaa
     1801 gctactggtt tctttgctaa gaaagaagca gctgaaaaac accttaaagg tggagctaaa
     1861 aaagttgtta tcactgctcc tggtggaaac gacgttaaaa cagttgtatt caatactaac
     1921 cacgacgttc ttgacggtac tgaaacagtt atctcaggtg cttcatgtac tacaaactgc
     1981 ttggctccaa tggctaaagc tcttcaagac aactttggtg ttgttgaagg attgatgact
     2041 actatccacg cttacactgg tgaccaaatg atccttgacg gaccacaccg tggtggtgac
     2101 cttcgccgtg ctcgcgctgg tgctgcaaac atcgttccta actcaactgg tgctgcaaaa
     2161 gctatcggtc ttgtaatccc agaattgaat ggtaaacttg atggatctgc acaacgcgtt
     2221 ccaactccaa ctggatcagt tactgaattg gtagcagttc ttgaaaagaa cgttactgtt
     2281 gatgaagtga acgcagctat gaaagcagct tcaaacgaat catacggtta cacagaagat
     2341 ccaatcgtat cttcagatat cgtaggtatg tcttacggtt cattgtttga cgcaactcaa
     2401 actaaagttc ttgacgttga cggtaaacaa ttggttaaag ttgtatcatg gtacgacaac
     2461 gaaatgtcat acactgcaca acttgttcgt actcttgaat acttcgcaaa aattgctaaa
     2521 taattcttga gttgatagaa agcaaggctt tgtggtcttg ctttttatat ggaaaaatgg
     2581 atgacacgat catccattct tttttaattc tttttcaaat gtatttgaaa gggtagtgaa
     2641 agttagcttc tctaaagtaa gtgggtgggt aaaggaaagt cggaaggcat gaagcataag
     2701 ccgacttgtc tttgatttac tattatagag agggtcgccc aggataggaa gattatgatg
     2761 cgaaaggtgc acacgaatct gatgggttcg ccctgtcttt agcttgcaac gagccaagga
     2821 agtcttgttt gagaattgct ttaatctgct tacatacgtt tcagcatatt gcccattttt
     2881 tgcatcaact attctttttc tacgatcatg gcgatcacgt ccaattttgt ctctgaaaac
     2941 aagttctttt ctgttgatat ttccatcaac tagagcccaa tattctctag aaatctcttt
     3001 tttctccaat aagcgattga gaatgggcag gataaaagga tttttggcaa agagaactaa
     3061 gccactggtt tccatgtcca gacgatgaac gacatagcag gtttggccaa cataggtact
     3121 gacatggtta agaagggcaa tttcgtttgg ttgattacca tgcgttttca tcccctctgg
     3181 tttgtttaca ataatcaagt attgatcttg ataaacttcc tgcactaagt ctgggttgcc
     3241 ccaagggatc gtcttttggg gataatcttc ctcgtcaaaa gtcaactggc aaacatctcc
     3301 aggatttacg atttcgttcc agtggacttc ttcttgattt atcaaaatat gtttcttgat
     3361 tctcaaaaaa tgacggattt ttctagggat gaggagttgt tcctcaagta attgctttac
     3421 cgtcatttga ggtagagagg cgggtaatgt aaatgtgaat tgcatacaga tattgtaaca
     3481 aaaaaagccc tatttggata ggaaatagct aaattcttgt cttcctatga tgaagatgat
     3541 aaaataaacg catgaaatta gataaattat ttgagaaatt tctttctctt tttaaaaaag
     3601 aaacaagtga actagaggac tctgattcta ctatcttacg tcgctctcgt agtgatcgaa
     3661 aaaaattagc ccaagtaggt ccgattcgaa aattctggcg tcgttatcat ctaacaaaga
     3721 ttatccttat actaggtttg agtgcaggct tgctagttgg aatctatttg tttgctgtag
     3781 ccaagtcgac caatgtcaat gatttgcaaa atgccttgaa aactcggact cttatttttg
     3841 accgtgaaga aaaagaggct ggtgccttgt ctggtcaaaa gggaacctat gttgagctga
     3901 ctgatatcag taaaaacttg cagaatgctg ttattgcgac agaagaccgt tctttctata
     3961 aaaatgacgg gattaactat ggccgtttct tcttggctat tgtcactgct ggacgttcag
     4021 gtggtggctc taccattacc caacagctgg ctaaaaacgc ctatttatca caggatcaaa
     4081 ctgttgagag aaaagcgaaa gaatttttcc ttgccttaga attaagcaaa aaatatagta
     4141 aggagcaaat tctaaccatg taccttaaca acgcttattt tggaaatggt gtgtggggtg
     4201 tagaagatgc gagtaagaaa tactttggag tttctgcatc agaagtgagt ctggatcaag
     4261 ctgcgactct ggcagggatg ctcaaggggc cggaactgta taatcccttg aattccgtag
     4321 aagattctac taatcggcgc gatactgtct tgcagaatat ggttgcagca ggatatattg
     4381 ataaaaacca agaaaccgaa gctgctgaag ttgatatggc ttcgcaattg cacgataagt
     4441 atgaaggaaa aatctcagat taccgttacc cctcttattt tgatgcggtg gttaatgaag
     4501 ctgtttccaa gtataatcta acagaggaag agattgtcaa taatggctac cgcatttaca
     4561 cagagctgga ccaaaactac caagcaaata tgcagattgt ttatgaaaac acatcgctat
     4621 ttccgagggc agaggatgga acgtttgctc aatcaggaag tgtagctctc gaaccgaaaa
     4681 cagggggagt tcgtggagtt gtcggtcaag ttgctgacaa tgataaaact ggattccgga
     4741 atttcaacta tgcaacccaa tcaaagcgta gtcctggttc tacaattaag cctttagttg
     4801 tttatacgcc agcagttgaa gcaggctggg ctttgaataa gcagttggat aaccatacca
     4861 tgcagtatga cagctataag gttgataact atgcagggat caaaacgagt cgagaagttc
     4921 ctatgtatca agccttggca gaatcgctta atctacctgc tgttgccgct gttaatgatt
     4981 tgggtgtcga caaggctttt gaggcaggcg aaaaattcgg actcaacatg gaaaaggtcg
     5041 accgtgttct tggtgtcgcc ttgggaagcg gtgttgaaac caaccctctt caaatggctc
     5101 aagcatacgc tgcctttgca aatgaaggtt taatgcctga agctcatttt attagtagaa
     5161 ttgaaaatgc tagtggacaa gttattgcga gtcataaaaa ttcacaaaaa cgggtgattg
     5221 ataagtctgt agctgacaag atgaccagta tgatgttggg gactttcacc aacggtaccg
     5281 gtattagttc atcgcctgca gactatatta tggcagggaa aactggaaca actgaagcag
     5341 ttttcaatcc ggagtacaca agtgaccagt gggtaattgc ttatactccg gatgtagtga
     5401 ttagccactg gcttggcttt cctaccactg atgaaaatca ctatctagct ggctctactt
     5461 caaacggtgc agctcatgtc tttagaaaca ttgccaatac cattttacct tatacgccag
     5521 gaagtacctt tacggttgaa aatgcttata agcaaaatgg aattgcacca gccaatacaa
     5581 aaagacaagt acaaaccaat gataatagcc agacagatga taatttgtct gatattcgag
     5641 ggcgtgcgca aagtctagta gatgaggcta gccgggctat ctcagatgcg aagattaagg
     5701 aaaaggctca aacaatatgg gattcgatag tcaatctatt tcgctaagat gcttgtcaaa
     5761 gcctagcttt cttgttataa tggataggat ggaggcgtta tggcactaaa aaaagcaagc
     5821 ctagcttgtg cggtttgtgg ttcgagaaac tattcaatca agatcagcgg aaaccccaag
     5881 cctacacgac tagaagtaaa taaattttgt aagcattgtg gcaagtacac tacacacaga
     5941 gaaacgagat aggagagagc gatgcgtttt attggagata tttttagact tcttaaagac
     6001 acaacgtggc caactcgcaa ggaaagctgg agagattttc gttctatcat ggaatacaca
     6061 gctttctttg tagtaattat ttacattttt gaccagttga ttgtttcagg tttgattcga
     6121 tttattaaca ttttttagaa gattagtgga gttaattaca ctagaaatct tctatattat
     6181 gaaaggaaat atcatggata gttttgataa agggtggttt gttttacaaa cttattctgg
     6241 ttatgaaaat aaggtgaaag aaaatctatt acaacgtgca caaacctaca atatgttgga
     6301 taatattcta cgcgttgaaa ttccaacaca aacagtgcaa gttgaaaaaa atggaaagag
     6361 aaaagaagta gaagaaaatc gctttccagg ttatgttctt gtagaaatgg tcatgacaga
     6421 tgaagcttgg tttgttgttc gaaacacacc aaatgttaca ggatttgtcg gatcacacgg
     6481 gaacagatca aaaccaactc cattattgga acaagaaatt cgtgacattt tggtatctat
     6541 gggacaaact gttcaagaat ttgatttcga tgttgagatt ggtcaaactg tacgtattat
     6601 tgatggtgct tttgcagact acactggtaa gattacagaa attgataata acaaagtgaa
     6661 aatgattatc tctatgtttg gtaatgacac agttgcagaa gtaaacctaa accaaatcgc
     6721 agaattataa ccctgagaga ggctggagcc tctctttttt gtgcagttta ggagctaaag
     6781 ggaacagaat ggagaaaatg taacaaatgt gttttctaat ctgttagact gtatctagaa
     6841 aggggaaaat tatgattaaa gaattgtatg aagaagtcca agggactgt
//
LOCUS       DAVBCV010000083         6834 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBCV010000083 DAVBCV010000000
VERSION     DAVBCV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.083"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(201..1625)
                     /locus_tag="VQ516_001962"
     CDS             complement(201..1625)
                     /locus_tag="VQ516_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2512350.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2169..4448)
                     /gene="pulA"
                     /locus_tag="VQ516_001963"
     CDS             complement(2169..4448)
                     /gene="pulA"
                     /locus_tag="VQ516_001963"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2512351.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4560..6518)
                     /gene="ligA"
                     /locus_tag="VQ516_001964"
     CDS             complement(4560..6518)
                     /gene="ligA"
                     /locus_tag="VQ516_001964"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2512352.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6611..>6834)
                     /locus_tag="VQ516_001965"
     CDS             complement(6611..>6834)
                     /locus_tag="VQ516_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2512353.1"
                     /translation="FFTQVQTKVESEFLGRVLSTIFTLAILFMPIAKGFMTVLPSVHL
                     SSFLIIGSGVIILSCISFIYVRTHFEKLI"
BASE COUNT         2022 a         1502 c         1239 g         2071 t
ORIGIN      
        1 actatatata gtaaaatgaa ataataacag gacaaatcga tcaggacagt caaatcgatt
       61 tctaacaatg ttttagaagt aaatctgtac tattctagtt tcaatatact ataataccaa
      121 aaagttcgta ttttttctat aattattatt attttatagc aaaaaataaa accaggaaaa
      181 caatttcctg attttacacg ttatttcaca tcaaaaacaa tggatttgac atttgtcatc
      241 gcttcaatgc tatatttaat tccttgcact ccagctccag aacctttgac accaaggaat
      301 gggaaattat ctggaccacg ctgggtttta ttattaatgt ggactgtacc tacttcaagt
      361 ttttcagcaa tttcaaaggc ttttttgaaa tcatttgtaa agactgatga ttgaaggccg
      421 aattcagatt cgttggcaaa ggcaatagct tcctctacac tagccacacg aatgattggt
      481 aaaacaggac caaatggctc ttcccatgcc actttcatat cttttgtaac ttggtcaaaa
      541 agcactggcc agaggagatt gccctcacgt ttgattggtg taagagcctg agcctctttt
      601 tcttgtgcat cctcaatcaa gccccaaatg aagtcggctg aagcattgtc aataacaggt
      661 gtaatatcag cattgtcaaa tggatcacca actgttaatt tagaaacttc ttcctgaagc
      721 aaagtagcta atttatctgc tacactttcg agaacaatga cacgtttaat ggccgtgcaa
      781 cgttgtcctg agtagctaaa ggctcccgca acaatttgct tggcagcatg ttccaaatct
      841 gcatcttcta gtacaagagc tgcatctttc ccaccaagtt ccaacatgat aggacgcata
      901 ccagctaaac gaccaatacg ttctccaata ggagttgaac ctgtaaagtt gatgaagttg
      961 acttctttgt gctcaatgat ataatcccca atttctgaac cacgacctgt aatggtgttg
     1021 aaaacacctg ccggaatccc tgcttcttca aatgctttag ccaacaagag tccagaaatg
     1081 gaaccttgtg ttggtggctt caacatgacc acattccctg caatcaaggc aggtgcaatt
     1141 ttagaagcag ataaattaac tggataatta aagggagcaa tcgctagcac gataccaact
     1201 ggttcacgac ggacaacagc cagtttgttt ttacttgctg cctcaaaacc accaccttcc
     1261 attgcttgtc cagtgatacg gagaccttcc tcagcagcat aacgaatcaa gtctgctgta
     1321 cgcactactt ctccaattgc tgctttaatc ccttttgcta cttctttggc aaggatagta
     1381 ccaatttttt ccttatcgcg ttctaaaata gctgctgttt tatgcaaata agccgcacgt
     1441 tcaattgctg ataaagctcg ccatgctggc agggctgcac gcgcagcttg catagcctca
     1501 tcagcttcag tctgagtcat ggctggaact gtacccaatt cttcttgatt gattggtgaa
     1561 taaatcgtaa tttcttgttc agatgatttc cattttccat ttactaaatt ctgatatctt
     1621 gtcaaattct tgtcctcctg aaaatgatta agatatattt taccaaattt tcagaaaata
     1681 acaacccttt ttgcaaaatt tttgagatta ttttcacaag cttgattttt caaagtatac
     1741 tcaataaaaa taaaaaaatc cactacgtca aggcgaggct aatgtggttt gaagaaattt
     1801 tcgaagagcg tgaatgagta tcatctatag taaaataaaa aaactgaaca atttggttgg
     1861 ggacagccaa accaatttct cacaatgttt cagaaacaag ggtgtgctat tccaatttca
     1921 gcctactata actgtcatag attgctgaaa caaagtctag gtaaaagtct tcataataaa
     1981 aagacctcct atcaaatgtt caaaaacttt gataggaggt cttgtttcgt gaaaatattt
     2041 atcaaatttt ctatacaagt gagctgttag ccaggttctt tctattcttt caatttcaat
     2101 gaatggattt tttaccaata ctcataactg ggaatttttc tgtctaaaaa tagcgagata
     2161 gatggtattt ataaaacact caagacagct agactaatat catttaaaac attatcttct
     2221 tttgagcgac tgttggttac caacatagct aaatttcctg cattttcaaa ttgatagggt
     2281 tctgatttag cattcacaac taccaagagg tgttctttgc cgtgaacttc atagataagg
     2341 cagccgctat gttcaatcgc agaatgcaca aagacatgat ggtaaatttc atcatagcta
     2401 gagtaagaaa aggcaccagt ttttgtcttc aatcggatga cttgacggat aaactcaata
     2461 ctgtcttgac gctcattaat caagttccag ttcacttggt tcacactgtc aggagcatta
     2521 tagctattca tcgcacgctc tctatcatca tgggtcaact caccattttc accagtcgca
     2581 accagtttgg tacgaccaaa ttcttgaccg atttccataa aggccatccc ctgcatgagc
     2641 agattcatgg ctgtggcagt ttcgaccttg cgcatgattt gctctgaact ttggtctgga
     2701 tgaagggttg ccaataaatc gtgaagattg taattgtcat gggcttctac atagttaagc
     2761 acctgatttg gatgtgtata gcttcctaat tcacgacttc ctaggattgc tttagctaga
     2821 attggctctg tcgcagcacc actgacaaaa cctgacttga tagcaccata aacttctccc
     2881 cctttgacag catcgcgctg attgtcatta aagaaaccaa tatttggcat ctggtaggca
     2941 ttgtccttct tggccttatc ataaggggca agacctgttc ccatatccca tccttctcca
     3001 tagaggataa tgttggagtc aatttcatcc aagctttgac gaatcatctg catggtcttg
     3061 acatcatgaa tccccatcaa gtcaaaacgg aagccgtcaa tattatattc ctgcacccag
     3121 tatagaagag aatcaatcat atacttgcga aacatttcgt gttcactggc tgtttcattt
     3181 ccaacacccg ttccattctg gaaggtacca tctggattca tacgataata gtaatcaggg
     3241 actgttgttt ggaatggtgc atcaacaact gagaaggtat ggttatagac tacatccata
     3301 atgactccaa tacccgcatc gtgataagct tgaaccatca ccttcaaatc acgaatgacc
     3361 tgagctggat catctggatt agttgaaaaa ctagtttctg gcgcgttata gttttgtgga
     3421 tcataacccc agttgtaggt tacatttcca tcctcatcgt attctttatg acggtctgca
     3481 attggttgca attgaacata attgtagccc agcttcttga tgtaatcaaa agcagttgac
     3541 tggccgtatt ggttaactgt tccagcctga gcagcaccca agaaagttcc tcgaagatgt
     3601 tcatctacac ccgatgtagg tgatttagtc aaatcacgaa tatgcatttc acagataact
     3661 gccttacatg gattttccaa gcgccaagta gcctccgaac cgtgcttaac ctcgaagttt
     3721 tcaacttgct tttctacatg gctcagaata gctgaacgtt tgccatcagg gctggtcgcg
     3781 attgtataag gatcacgtgt cagtgtttgg tgatgaggga attggacttg atactgataa
     3841 gtcttaccta ccaaatcttc ttcaacatcc aaactccaga caccgattgt attgtcctta
     3901 tgattataag agtagctatt gcctcttttc atctcaaaag tcttccaaac gggtgcatca
     3961 ttagcagctg attcataaac gacaacttgc acttctgtcg ctgtaggtga ccagagggaa
     4021 aaatgagcct gattgtcctc tacacggcaa cccaattctc cttggtaacc ccaatgatga
     4081 tcaaaactag cactgttaat ggccttatca aaggcaaaag gattttgatt tttatagaaa
     4141 ggactggcaa tagcaggatt ttcagagtaa taaatcctat catcgccttc caaaatccag
     4201 acctctgtta ataggggata gtgattaaaa cggatagaat attctttact agtttgacct
     4261 gtatgaacca caaaattcaa gctttctata acatgtgaac ttgggtgttc aaagctaaat
     4321 aaagctccaa aataatcttc tttgtaggtt agcaaatcaa ttcgttgatc ctgacttttt
     4381 acaaaggagc aagtgtcata ttctccattc ttacgatggt aatgaatgcg catagggtag
     4441 ttatacattt tttatttttc ctttttactt tgtttctatt tcactaataa atttttgtca
     4501 atctcgtctc aattaacaga catagtcata ttctctaaac tctgttttta aacgatccat
     4561 tacaaacttt ctagccatgc ctcatctctg acctggatac caagttcttg tgctttttgc
     4621 agtttacttc cagcgtctgc acctaccacg acgaggtcgg tctttttaga aacactacct
     4681 gtcactttgg cacccagact ttcgagttta cttttagctt ctgagcgctt gagtcgttcc
     4741 aattttcctg tcaataccac ggtcaaacct gacaaggccg catccgctac taccgtctgt
     4801 cctttatagt ccagattgac cccagtttct ttcaattctc tgagcagaat ttcagagcct
     4861 tctgtcgcaa aataagtctg aagacttttg gcaatcacgc cacctagact ttcaatacta
     4921 gccacttcct ctgaatctgc ctgagacaga ttttcaattg aatggaaata ttgaagtaaa
     4981 agctgactag ccttgcttcc gacatgacga attcccaaac caaataagag cttctcggca
     5041 gaattttcct ttgatgcttg gatagcctga tacagtttag cagcggactt ttccttaacc
     5101 ccctctaaaa ggaggaaatc ctcttcttgc aaacgataaa tatccgccac atccttgact
     5161 aaattagcag caaaaagctt ctcaacaata gatggaccaa ggcctgtaat attcatagca
     5221 tcacgagaag caaagtgaat caagccttcc atgatttgag cagggcaacg cggattgata
     5281 caacgtaggg ccacttcatc ttcaaagtgc aacaagtcag agttacaact tggacagttt
     5341 gtagggatat ctagtttttc ttcagaaacc cgtttggact ctaccacacg taaaacggca
     5401 gggatgatgt caccagcctt atatacaatg accgtatcgt cttttcggat atctttttca
     5461 gcaatataat ctacattgtg cagggtcgca cggctaacag tcgtaccggc aagttgtact
     5521 ggtgttagat tagcagttgg agttacaaca ccggtacggc caactgtcca gtcaactgat
     5581 aagagttgag cttctttttc ttcagcaggg aacttgtagg ctactgccca ctttggagcc
     5641 ttaacggtaa aaccaagttc ttcttgactt gctaggtcgt tgaccttgat taccactcca
     5701 tcaatatcgt aaggcagatt ttcccgttcc tgtcctactt cttggataaa attccagatt
     5761 tcatctatgt tttcagccaa gattcgctta ggattgacca caaaacctag ttgttctagg
     5821 tacttcaaac ccttttcttg gctatcacga gttgaagggc tggcttcttg atagagaaac
     5881 gttgcaagat tacgcttggc aactactgct gtatccaact gacgcagagt tcctgctgcc
     5941 gcattacgag gattagcaaa ttcaggctct ccattttctt ggcgcacttg gttaacttgg
     6001 tcaaaggaag cgcgtggcat gtaacattcc ccacgaactg tgatatctag ttcttctggc
     6061 aaagtcaaag ggatgtcctt aacacgcttg aggttttctg tgatattttc accaattgaa
     6121 ccatctccac gtgttacccc agcaaccaaa atcccctttt cataagtcag cgagatagat
     6181 aagccatcga ttttcagctc acaaatatag gtcggatgag ccacttcctt acgaacacgc
     6241 gcatcaaaag catctagctc ctcacgtgaa aaagcatcct gcaaactata aagaggatac
     6301 tgatgactgt atttttcaaa accatctaaa accttgccac caacacgatg agtcggactg
     6361 tctgctagca cttgctctgg ataagcagtt tctaactcga ccaactcacg gtaaaggcgg
     6421 tcatactcac tgtctgaaac cgagggatta tcgctggtat agtactcagt cgcatagcga
     6481 ttgagcaaag cgactaactc attcattctt ttattcataa gaccatttta ccataaaaca
     6541 agccctcctc acaaacgaga agggcggaaa aaacacttag tttgaaatta tttttgaaac
     6601 tcaagcaacc ttatatcaat ttttcaaaat gagttcgaac ataaataaac gatatacaag
     6661 acaagatgat aacaccactt ccaattatca ggaaagaaga gagatgtaca cttggcaaga
     6721 ctgtcataaa tccttttgca ataggcataa atagaatagc taaggtaaaa attgtactca
     6781 gtactcttcc aagaaattcg ctctcaacct tggtttgtac ttgagtaaaa aagt
//
LOCUS       DAVBCV010000084         6637 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBCV010000084 DAVBCV010000000
VERSION     DAVBCV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.084"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(84..1244)
                     /locus_tag="VQ516_001966"
     CDS             complement(84..1244)
                     /locus_tag="VQ516_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2512354.1"
                     /translation="MKKKFALSFVALASVALLAACVEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTNMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(1345..1593)
                     /locus_tag="VQ516_001967"
     CDS             complement(1345..1593)
                     /locus_tag="VQ516_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2512355.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQ516_001968"
     CDS             complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQ516_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2512356.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQ516_001969"
     CDS             complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQ516_001969"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2512357.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3154..3621)
                     /locus_tag="VQ516_001970"
     CDS             complement(3154..3621)
                     /locus_tag="VQ516_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2512358.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(3640..4197)
                     /locus_tag="VQ516_001971"
     CDS             complement(3640..4197)
                     /locus_tag="VQ516_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2512359.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            4322..5167
                     /locus_tag="VQ516_001972"
     CDS             4322..5167
                     /locus_tag="VQ516_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2512360.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            5226..6398
                     /locus_tag="VQ516_001973"
     CDS             5226..6398
                     /locus_tag="VQ516_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2512361.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            6449..>6637
                     /locus_tag="VQ516_001974"
     CDS             6449..>6637
                     /locus_tag="VQ516_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2512362.1"
                     /translation="MEHIILLRGVTPNGKNAIPKMSYLVDILTEADFQQVRTYIQSGN
                     IILESNLALEEIREQVHTL"
BASE COUNT         1894 a         1385 c         1287 g         2071 t
ORIGIN      
        1 agtttgaaga gattttcgaa gagtattaaa tatcgaaaca gggagtgagt caaaggctca
       61 ttccctattt caacattttt ctattatggt tttacaactt ctgctgcttc aactttacca
      121 ttgttcatgg tcatcatgta agcagttttg actgtgttgt ggtctgcatc gaagcttgtt
      181 tgaccagtta caccttcaaa atcttttgtt ttagcaaggt tatccttgat ttcacctgaa
      241 tttttagcac cttttgctgc gtttgctaca aggtgaactg aatcataagc caaggctgca
      301 aatgttgaag gctcttcatt gtacttagca cggtaagcgt caaggaaggc tttagcttta
      361 gctgaaactt ctacagtagt tgagaagcct gagataaagt agatgtttga tgctttttca
      421 gcagttgctt gttgtacaaa ctcctcaccg ttgaatccat caccaccaac gattggtttg
      481 tcaattccca tgccacgcgc ttggtttaca atcttaccag cctcattata gtaaccagga
      541 acaacgatag catcaaagtc tttccctttc atgtttgtaa gggctgcttg gaagtctgtg
      601 tcacctgcta cgaaagtttc atctgcaacg atttcaccct tgtatgactc gcggaaagat
      661 ttagcaatcc ctttagcata gtcactggca ttgtcagtgt aaagaacaac tttcttagca
      721 tttaattttt cagaaacata gtttgagata atttttcctt ggaagctatc ttggaaagtt
      781 ccaataaaga ggtaatcttg acctttagtc aatccatctt gagtcgcact tggtgagatc
      841 aatggaacac ctgcttttgt agcgttcgct accgcagctg cagtcgcacc agatgtcgca
      901 ggtcctacga ctgctgatac tttagattgg gttacaaggt tagttgtaac tgaagcagcc
      961 tcagctgttt cagacttatt atctttatcg actacttcga tttgttttcc atcgatacca
     1021 cctgcggcat tgatttcatc aacagccaat tgggcacctt tttgttcagc tgttccgtat
     1081 gcagctaaag aacctgattc ttcaaagtta aacccgattt taattgtctt ttcctctact
     1141 gagttaccag cagtgttgac tgctccagac ttcacttcta cacaggctgc aagaagtgct
     1201 acacttgcaa gcgccacaaa cgatagggca aattttttct tcatcttttt gtctccttat
     1261 ttcttaaata gtctgttaag aatataccga attatcagaa aattgtcaat gttttccttg
     1321 aactttttcg atgataacgt tttcctatcg ataaagattg cccacaaagg gtgtttccaa
     1381 ctcttggata tgacaaaccc taactttttt aataaatttt tccttgctca agcgtccgac
     1441 caattgctcc acttcttgag ttggaacata gagttgtaag taacgatgtt tcttggaatg
     1501 ataggtaata tctccataat cctgcagttt tttggcatca cgattatagt aaagatagat
     1561 aatcaagcca gagcgattga ctttttcaaa catgataaat cctctttcta agaaatctct
     1621 ccctattata ccaaaaaaag caaacccagt cgagtttgcc ttctatcatc attagttcaa
     1681 tgaattgttg gccataattt catcaataaa gccatattca agtgtttcct gggcgctcat
     1741 ccagttatca cgttctgcat ctgcatggac tttttccatt gactgacctg aattttcagc
     1801 caagattttt tccaaggtat tacgagtttt gagcaagtgt tctgcagcga tagccatatc
     1861 agtttgttgg gtaccaccac ctgtaccgcc cattggttgg tgaatcatgt attcagcatt
     1921 tggaagcatg aaacgtttgc cttttgctcc acttgatgcg atgactgttc ccatagatgc
     1981 agccattccc ataacaatgg tttggacatc tgccttgata aagttcatgg tatctacgat
     2041 tgccaaacca gctgaaacag aaccacctgg tgtattgaca taaaggtaaa tatcttttgt
     2101 actatcttgg gcatccaaga aaagcagttg ggcaataaca gagttagcca tattgtcttc
     2161 aaccggacct gtcagcataa tgatgcggtc tttgagaaga cgtgagtaaa tatcgtaaga
     2221 acgttctcca cggcttgttt gttcaataac tacaggaatc attcatttct ccttttgagt
     2281 tttaattttg ttggtcaaat gactgaagat aagactatta taatatcttg gtcaaaaaag
     2341 gtcaaatttt tgctctgctt tcattagaca gaaacaaaaa cccaacctcc tttcgtgact
     2401 ggaaatactt ttccaagtca ttcttctttt cgatcttatt ttgtaccgaa caagcggtct
     2461 ccagcatctc caagacctgg aacgatataa ccgtgttcgt tcaaacgttc atccaaggct
     2521 gctgtaaaga tttctacatc tggatgagct tcttgaaggg cttttacacc ctctggagca
     2581 gatacaaggc agacaaattt gatatttgat gcgccacgtt ttttaagaga atcaacagcc
     2641 aagattgctg agccacctgt tgccaacatt gggtctacta caaaaatttg acgttggtca
     2701 atgtcctcag gcaatttcac caagtattca actggttgaa atgtttcttc atcacggtac
     2761 ataccgatgt ggccaacttt agcagctgga accaagctca agagaccatc aaccatcccg
     2821 atacctgcac gcaagattgg gacgatggcc aatttcttac ctgccaattg tttttgaact
     2881 gtttttgtaa ttggtgtttc gatttccaca tcttctagtg gaagatcacg aagtacttca
     2941 taccccatca acattgcaat ctcatctact agctcacgaa aagcttttgt agaagtatct
     3001 gtacgacgca agattgacaa tttgtgttga atcagtgggt gattaataac ttcaattttt
     3061 cccatttttg gaattccttc tttcaattta ttcttcttat tataccaaaa aacgggttaa
     3121 aaatctttct aaaccattta tttttgataa tttttacatt agatcagcct ctttaagagc
     3181 tgtctgtact gtctcaagtg gtaaatgggt caattctgtc cctttttctt gataaaggta
     3241 ttgggcgtag tcgtccattc ggtactggtt gatataaacc acacgcttgc agccgacctg
     3301 aagcaattgt tttgtacagt tgagacaagg aaaatgggtt acataggctg taaagccttt
     3361 gggaacacca cgctcagcac cttgaaggat agcattgacc tcagcgtgaa gggtgcgaac
     3421 acagtggcct tcaatgacca aacattcgtg atcaatacaa tgctcagtcc ctgacaccga
     3481 accattgtaa ccagtggaaa taaccttatt atcttttacc agaatcgcgc ccactttagc
     3541 acgtttacaa gtggaacgat tcgcaattag tagagcttgg gctgcaaaat actcatccca
     3601 ggccagtctt ttttcagtca tctcttttct cctttttctc tattttttaa aaaatggtaa
     3661 acctaaatct gcaatctttt cagctggtac cttcatgcca tccttgatcc attttagaag
     3721 gacagagacg atggctgagc tccagaagga atgaagataa gagctgacac cttttgattt
     3781 cccatggtat ttttctagaa attcctgcat ggcttggaca aagatttttt ccagatggta
     3841 atccaaggcc aattgaatta ctctagcttc ctttctggcc tcccggaaaa ggtgaaccca
     3901 aaccaaataa aggtctgtct ttaaatcgta atgatgcagc tgttccataa tattgtggac
     3961 agttcgttta aagacgctct ctaaaatttc ctctttggag tcataattgc gataaaaggc
     4021 cgcacgcgaa acacctgcac gtttgaccaa ttcagaaata ctaatcttgg tcagttcctt
     4081 tttttccaag agttgcaaga gggctgtttc aatggcttct ctggttaata aattggattc
     4141 ttggtttgat tttctgagat tttcaagaga cttttcagag attctacgtt cagacataac
     4201 attttctttc tacttgtcac aacagacgga tgatgctttt gtttcaaggg ttttcatgat
     4261 ataattgtaa aaaaagacag aacctttgtc aatgtataac tgacaaagat ggagaatttc
     4321 tatgacttgg aagattattg ctgactctgg ttgtgattat cgtcaactgc caacaccagc
     4381 tattaacaca acctttgtaa gtgtcccctt aaccattcaa gtagctgatc aggtctttgt
     4441 tgatgacgcc agtctcgata ttgaccaaat gatggaaacc atgtatgcaa ctgcagaagc
     4501 ttcaaaatcg gcttgtccaa gcccagatga ctatttgcga gcatttgaag gtgccaaaaa
     4561 cattttccta gtaaccatca caggtaccct ttctggcagt cacaatagtg ctcaactagc
     4621 aaagaatatt tatctggaag accatcctga cactaagatt catgtgattg atagtttatc
     4681 tgctggtggt gaagttgacc tactcgtaga aaaattgaat gacttgattg accagggctt
     4741 gtcttttgaa gaagtggttg aagctatcac cgcctatcaa gaaaaaacta agttgctctt
     4801 tgtcctagcc aaagtcgata acttggtgaa gaacggccgt ttgagcaagc ttatcggtac
     4861 ggtcgttggc cttctcaaca ttcgtatggt cggaaaagct agtgaaactg gaactctcga
     4921 attgctacaa aaagcaaggg gatcaaagaa atcagttcaa gctgcctatg atgagttagt
     4981 aaaagctgga tatgctggtg gccgtattgt catggcccaa cgcaataacg aaaaatgttg
     5041 tcaacagctc tcagagcgaa tccgtgaaac cttcccacaa gcggatatta aaattctacc
     5101 aacctctggt ctctgcagtt tctatgcaga agatggcggt ttgctgatgg gctatgaaat
     5161 tgattaattg tattcttgac aagaggtgac catcatctct tgtttttttg tggtacaata
     5221 gagctatgaa acattttgat actattgtca tcggtggggg acctgctggt atgatggcta
     5281 cgatttccag tagcttttat ggacagaaaa ccctcctcat cgaaaaaaat cggaaacttg
     5341 gaaaaaaatt agctgggact ggtgggggac gttgcaatgt gaccaacaat ggtagcttag
     5401 acaacctgct agctggaatt cctggaaacg gacgctttct ttacagtgtt ttctcccagt
     5461 tcgataatca tgacatcatc aactttttta cagaaaatgg tgttaaactt aaggtcgaag
     5521 accacggacg cgtctttcca gccagtgaca agtctcggac tattatcgaa gctttggaaa
     5581 agaaaatcac cgaactaggt ggtcaagttg ctactcaaat agaaatcgtt tctgttaaaa
     5641 aagtagatga ccagtttgtc cttaagtcag cggatcaaac cttcacttgt gagaaactca
     5701 ttgtcacaac aggtggtaag tcttatcctt cgactggttc gactggtttt ggtcacgaga
     5761 ttgctcgcca ttttaagcat accatcaccg atcttgaggc tgctgaaagt cctttattaa
     5821 cagattttcc acataaagcc ttacaaggga tttctttgga cgatgtgacc ctaagttatg
     5881 gtaagcatgt catcactcat gatttactct ttacccactt tggtttgtca ggtcctgctg
     5941 ccctacgcat gtctagcttt gtcaaaggtg gggaggttct ctcactcgat gttttgcctc
     6001 aactttctga gaaggacttg gttacatttc tagaagaaaa tcgggaaaaa tccttgaaaa
     6061 acgctttaaa aaccttgtta ccagaacgct tggccgaatt ttttgtacaa ggatatcctg
     6121 aaaaagtcaa acaactgact gaaaaggaac gagaacaact tgtccagtcc attaaagaac
     6181 ttaaaattcc tgtaactgga aaaatgtccc ttgcaaagtc ctttgttacc aagggtggag
     6241 tcagtctcaa ggaaatcaat cctaaaaccc ttgaaagtaa gctggtacct ggcctccact
     6301 ttgcaggcga agttatggat atcaatgccc acacaggtgg ctttaacatc acttctgccc
     6361 tctgtaccgg ctgggtggcg ggatcaaatc caatctaaat atacattact aaatggaaca
     6421 atcccccaaa aaaaagaaag gaactacttt ggaacatatt atcttgctaa ggggagtcac
     6481 tcctaatgga aaaaatgcta tccctaaaat gtcttatcta gtagatatct tgacagaagc
     6541 tgattttcaa caagttcgaa cttatattca aagtggaaat attattcttg aaagtaactt
     6601 agctctcgaa gaaatacgag aacaggttca tactcta
//
LOCUS       DAVBCV010000085         6447 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBCV010000085 DAVBCV010000000
VERSION     DAVBCV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.085"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            222..422
                     /locus_tag="VQ516_001975"
     CDS             222..422
                     /locus_tag="VQ516_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512363.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            447..863
                     /locus_tag="VQ516_001976"
     CDS             447..863
                     /locus_tag="VQ516_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512364.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1094..1801
                     /gene="ccdA2"
                     /locus_tag="VQ516_001977"
     CDS             1094..1801
                     /gene="ccdA2"
                     /locus_tag="VQ516_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2512365.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1812..2369
                     /gene="sdbB"
                     /locus_tag="VQ516_001978"
     CDS             1812..2369
                     /gene="sdbB"
                     /locus_tag="VQ516_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2512366.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2490..3881
                     /locus_tag="VQ516_001979"
     CDS             2490..3881
                     /locus_tag="VQ516_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2512367.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPSWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4024..4941
                     /gene="adcAII"
                     /locus_tag="VQ516_001980"
     CDS             4024..4941
                     /gene="adcAII"
                     /locus_tag="VQ516_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2512368.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            4949..>6447
                     /locus_tag="VQ516_001981"
     CDS             4949..>6447
                     /locus_tag="VQ516_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2512369.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT         1980 a         1172 c         1354 g         1941 t
ORIGIN      
        1 atggtttcag aaatttctct taccaacatt aaccacacca tcggttatta tagtaaaatg
       61 gaataagaac aggacaaatc gatcaggaca gtaaaatcga attctaacaa tgttttagaa
      121 gtctatatgt actattctag tttcaatata ccataactgc tcctagtcaa cctgcaagtc
      181 ctgtcccagc tccaagctca gctccgactc aagatcctag agtggtagca agcgcttcgg
      241 ctagctcaac gagcacacaa gctcaagagc aagttgacaa gtctgaactt cgtgccttga
      301 gtcaagagtt agaccaacgc ttgaaagctt tggcaacagt atctgatcca aaaatcgatg
      361 caaccaaggc tgtcctccta gatgctcaaa aagctccgga agatagtgcc ttgacagagt
      421 aaggtctgca tactgcagta gagtctgtga aggctgcctt ggactctctc aaagatgtga
      481 aagcaaatgc tagcgacagc aagcctgcac aggacaagaa ggatgcaaaa caaggaacgg
      541 aagatagtaa ggattcagat aagatgactg aaacaaactc agttccggca ggagtgattg
      601 tggtcagtct acttgccctc ctaggcgtga ttgccttctg gctgattcgc cgtaagaaag
      661 agtcagaaat ccagcaatta agcacggaat tgatcaaggt tctaggacag ctagatgcag
      721 aaaaagcgga taaaaaagtc cttgccaaag cccaaaacct tctccaagaa acccttgatt
      781 tcgtgaaaga agaaaatggc tcagcagaga cagaaactaa actagtagag gagcttaaag
      841 caatccttga caaactcaag taaaacatga tttatgaaat gtcagaccta aaagacattt
      901 tcgttcacaa aagcagtgac gagtaatcct tgtcgttgct ttttggctat tttgggggat
      961 aagatccgcc ttgactagca aaagaaaaga ttggtccaat agtgcccgat acgattttgc
     1021 gtagcagaaa gcttacaaat aaaattgaat atagtaaaat aggattagaa ttattaagaa
     1081 agttggttct ttattggaaa cgatagtatt tttaatctct gtttttctag caggtgtttt
     1141 atcctttttt tctccttgta tttttcctct tctgccagtc tatgctggga ttttattgga
     1201 tgatcaagaa agtgcaaaaa gcttttcttt gtttgggaga aaggttctct ggtcaggctt
     1261 gattcgaaca ctttgcttta tcgctggtat ctctctcatt ttctttattc taggctttgg
     1321 tgctggttac tttggtcata ttctctatgc aaattggttt cgatatgtca tgggagctat
     1381 tattatcatt ttgggtcttc accagatgga aatttttcat ttgaagaaat tagaagttca
     1441 aaaaagtttt acctttaaaa aatcagattc taatcgttat tggtcagctt ttttacttgg
     1501 tattaccttt agctttggtt ggacaccttg tattggtcca gttttaagtt ctgttttagc
     1561 acttgcggct tctggaggca atggtgcttg gcaaggagcg atttatactc tcatttacac
     1621 tctgggcatg gcccttcctt tcttggtctt ggcactagct tcaggtctag tcatgccata
     1681 ttttagtaaa atcaagcgtc atatgatgct actaaagaaa attggtggtt tcctcattgt
     1741 tttaatggga attttgttac tattaggaca agtaaatgtt ctagctggaa tttttgaata
     1801 aaggaggaca aatgaaaaaa gtaatgtttg ctggcttaag tctcttgtca ttagttgtat
     1861 tgatggcctg tggtgaggaa gaaactaaaa agactcaagc agcacaacag ccaaaacaac
     1921 aaacgactgt acaacaaatt gctgttggaa aagatgctcc agacttcaca ttgcaatcca
     1981 tggatggcaa agaagttaag ttatctgatt ttaagggtaa aaaggtttac ttgaagtttt
     2041 gggcttcatg gtgtggtcca tgcaagaaaa gtatgccaga gttgatggaa ctagcggcga
     2101 aaccagatcg tgatttcgaa attcttactg tcattgcacc aggaattcaa ggtgaaaaaa
     2161 ctgttgagca attcccacaa tggttccagg aacaaggata taaggatatc ccagttcttt
     2221 atgataccaa agcaaccacc ttccaagctt atcaaattcg aagcattcct acagaatatt
     2281 taattgatag ccaaggaaag attggaaaga ttcaatttgg tgctatcagt aatgcggatg
     2341 cagaagcagc atttaaagaa atgaactaga atcgataaaa tctgattaca agatgtagtc
     2401 agattttttt agaaaaacat tttataaata ttcacatatc tcctatattt atggtaaaat
     2461 agaattatca gtttattttg gagtcaaaga tgaatatatt tagaacaaag aatgttagtt
     2521 tagataaaac agagatgcat aggcatttga agttatggga tttgattttg ctgggtatcg
     2581 gagccatggt agggacaggc gtctttacaa tcacaggtac tgcagctgca acacttgctg
     2641 gcccagccct agtgatttca atcgttattt ctgccttgtg tgtgggatta tcagccctct
     2701 tttttgcaga atttgcctcg cgagtacccg ctacaggagg tgcctatagt tacctctatg
     2761 ctatcttagg agaattccct tcctggttgg ctggttggtt aaccatgatg gagttcatga
     2821 cagccatatc aggcgtagct tcgggttggg cagcttattt taaaggcttg ctctctcaat
     2881 acgggatagc ctttcctcag gctttaaatg gtacctttaa tcctcaagca gggacatttg
     2941 ttgatttatt gcctattctt gtcttggtct tggtgacttc gcttgtttta ctaaatgcca
     3001 aggcagcctt acgctttaat tcgattctag ttattttgaa attttccgct ttagctctct
     3061 ttgttttagt aggaatttgg aatattaagt ttgataattg gagcaatttt gctccttacg
     3121 gttttggaca aatctatgga gctagcactg gtattatggc tggtgcgtcc ttgatgttct
     3181 tcggtttttt gggatttgaa tccatctcta tggctgtaga tgaagtcaag actcctcaaa
     3241 aaaatattcc tagagggatt gtcttatcgc tttctatcgt aaccattctc tatgccttgg
     3301 tgacccttgt tttgactggt gtggttcact atagtcattt aaatgtcgat gatgccgttg
     3361 cctttgccct tcgtagtgtt gggattagtt gggcagccaa ctatgtgtca ttagtggcta
     3421 tcttgacctt gattacagtt tgtatttcga tgacctatgc cctatcgcgt atgatttaca
     3481 gtttagcacg tgacggctta gtgcctgctg cctttaagga actgacgaag actagcaaga
     3541 taccaaagaa tgctactatc ttgacaggtc tagcttcagc agtagcagca ggaatgttcc
     3601 cactagccag tattgcagcc tttttaaata tttgtacctt agcctacttg atcatgctgg
     3661 cttatggtct gattcgctta cggaaagaaa aaggaatgcc caaagctggg gaatttaaaa
     3721 caccattggt acctctgtta ccaattttat cgatcatcat ttgtttatcc ttcatgttac
     3781 agtataatat gaacacctgg attgctttcc tagttgcgtt gttagtagga agtattattt
     3841 acttcactta tggctataag cattctacca tagaagaata aagtgaaagt ctattgagtt
     3901 gttgacactc agtagatttt tatatgtgaa agaaatttca aattttttct aaaaatatct
     3961 tgacagatta aattttcagg agtagaatat ttactagtta attaaatggt taaggagttg
     4021 ttcatgaaga aacaaaattt atttttagtc ctgttaagtg tctttctttt gtgtttgggg
     4081 gcttgtggtc aaaaggaaag tcagacagga aaggggatga aaattgtgac cagtttttat
     4141 cctatctacg ctatggttaa ggaagtatct ggtgacttga atgatgttcg gatgattcag
     4201 tcaagtagtg ggattcactc ctttgaacct tcggcaaatg atatcgcagc catctatgat
     4261 gcagatgtct ttgtttacca ttctcataca ctcgaatctt gggcaggaag tctggatcca
     4321 aatctaaaaa aatccaaagt gaaggtctta gaggcttctg agggaatgac cttggaacgt
     4381 gtccctggac tagaggatgt ggaagcaggg gatggagttg atgaaaaaac gctctatgac
     4441 cctcacacat ggctagatcc tgaaaaagct ggagaagaag cccaaattat cgctgataaa
     4501 ctttcagagg tggatagtga gcataaagag acttatcaaa aaaatgcgca agcctttatc
     4561 aaaaaagctc aggaattgac taagaaattc caaccaaaat ttgaaaaagc gactcagaaa
     4621 acatttgtaa cacaacatac agccttttct tatctagcga agagatttgg gcttaatcaa
     4681 cttggtattg caggtatctc tcctgaacaa gaaccaagtc cacgacaact aacagaaatt
     4741 caggaatttg ttaagaccta taaggttaaa acgattttta cagaaagtaa cgcttcttca
     4801 aaagtagctg aaactcttgt caaatcaaca ggtgtgggtc ttaaaactct gaatccttta
     4861 gagtcagacc cacaaaatga caagacctat ttagaaaatc ttgaagaaaa tatgagtatt
     4921 ctagcagaag aattaaagtg aggaaagaat gaaaatcaat aaaaaatatc tagcaggttc
     4981 agtggcagtc cttgccctaa gtgtttgttc ctatgaactt ggtcgtcacc aagctggtca
     5041 ggttaagaaa gagtctaatc gagtttctta tatagatggt gatcaggctg gtcaaaaggc
     5101 agaaaatttg acaccagatg aagtcagtaa gagagagggg atcaacgccg aacaaattgt
     5161 tatcaagatt acggatcaag gttatgtgac ctctcatgga gaccattatc attactataa
     5221 tggcaaggtt ccttatgatg ccatcatcag tgaagaactt ctcatgaaag atccgaatta
     5281 tcagttgaag gattcagaca ttgtcaatga aatcaagggt ggctatgtga ttaaggtaga
     5341 cggaaaatac tatgtttacc ttaaagatgc agcccatgcg gacaatattc ggacaaaaga
     5401 agagattaaa cgtcagaagc aggaacgcag tcataatcac aactcaagag cagataatgc
     5461 tgttgctgca gccagagccc aaggacgtta tacaacggat gatgggtata tcttcaatgc
     5521 atctgatatc attgaggaca cgggtgatgc ttatattgtc cctcacggca atcacttcca
     5581 ctatattcct aagagtgatt tgtctgccag tgaattagct gctgcccaag cctattggaa
     5641 tgggaagcag ggatctcgtc cttcttcaag ttctagtcat aatgcaaatc cagctcaacc
     5701 aagattgtca gagaaccaca atctgactgt cactccaact tatcatcaaa atcaagggga
     5761 aaacatttca agccttttac gtgaattgta tgctaaaccc ttatcagaac gccatgtaga
     5821 atctgatggc ctagtatttg atccggcaca aataacatat cgtacagtca atggtgttgc
     5881 tgtaccgcac ggagaccatt atcactttat tccttattca caactgtcac ctttggaaga
     5941 aaaattggct cgtatgattg ctgttaaggg acaaaatggt gcagtcttgc caggaatgca
     6001 ttatttgaaa ccagcaccaa aaccgcaagt tcaaccatca actgagaaaa aacaaactga
     6061 ttttgcagta gaacaggttg ttagaaaagt tggagaaggc tatgttgtgg aaattgctgg
     6121 tgtcagtcac tatgtttttg ctaaagactt ggctaaggat aagattgatg ctattgaaaa
     6181 tctcttgtct aagaaaactc aagagacaca tgctctagtt gctaaaaaag aaaatgtcgc
     6241 tcctcgtgac caagaatttt atgataaagc ttacaatctg ttaacccagg ctcataaagt
     6301 cttgtctgaa aataaaggtc gtacttctga tttccaagcc ttagacaaat tagcagaacg
     6361 cttgaataat gaatcttcta ataaagtaaa attggtagat gatttattgg cattcctagc
     6421 accaattacc cacccagaac gccttgg
//
LOCUS       DAVBCV010000086         6187 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBCV010000086 DAVBCV010000000
VERSION     DAVBCV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.086"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(119..751)
                     /locus_tag="VQ516_001982"
     CDS             complement(119..751)
                     /locus_tag="VQ516_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2512370.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ516_001983"
     CDS             complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ516_001983"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2512371.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ516_001984"
     CDS             complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ516_001984"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2512372.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2567..3139
                     /locus_tag="VQ516_001985"
     CDS             2567..3139
                     /locus_tag="VQ516_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2512373.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3191..4054)
                     /locus_tag="VQ516_001986"
     CDS             complement(3191..4054)
                     /locus_tag="VQ516_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2512374.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            4520..4858
                     /locus_tag="VQ516_001987"
     CDS             4520..4858
                     /locus_tag="VQ516_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2512375.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            4836..5195
                     /locus_tag="VQ516_001988"
     CDS             4836..5195
                     /locus_tag="VQ516_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512376.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WILPILLMAGMGDVIIN"
     gene            5214..5426
                     /locus_tag="VQ516_001989"
     CDS             5214..5426
                     /locus_tag="VQ516_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512377.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            5841..5909
                     /locus_tag="VQ516_001990"
                     /pseudo
     CDS             5841..5909
                     /locus_tag="VQ516_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            5889..>6187
                     /locus_tag="VQ516_001991"
     CDS             5889..>6187
                     /locus_tag="VQ516_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2512378.1"
                     /translation="MMYLDGGGVGCNWWNKRDNIAAAAVSGGMSVYQTYAIKKLIANR
                     GMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDG"
BASE COUNT         1901 a         1050 c         1311 g         1925 t
ORIGIN      
        1 atctacaacc ttaaaacagt gttttgagca gcccgcagct agtttcctag tttgctcttt
       61 gattttcatt gagtattact tcattttctt ctgaaataga gttgtcgccc agtctatgct
      121 attgaaaata cgccaaaact tctaagggtt tgtgagcgat ataatcaggt tgatagttta
      181 gtagatctgc ttgctctcca aatccccaag tgacggccaa tttctgaata cctgtttctc
      241 gagctcccag catatcaaac ttggtatctc cgatgatgat ggcttgttct ggtgctagtt
      301 gatgtgtctg taaggcttgg tgaatgacat ctgccttatg gggtgcttca gggctagaac
      361 cataaatacc atcaaagaaa tgatggattt ccaagttttt ggccatgtct tgagcagtag
      421 atgtattctt tgtcgtggtg atgtagagtg gataactgct cgataactcc tcaagcaagt
      481 ctataatctg aggaaagagt tgagcttcat agatgccttt tgccttatag taagaacgat
      541 atatctgcat ggcttcagaa atttggtctt tggacaggca ggtcgcaaaa ctactttcga
      601 gaggtggtcc cataaaacca cgaatagttt tggcatcagg gctaggcacc cccagctctt
      661 taaaggtata ggtaaaggca ttgtgaatcc cgatagaact atcaacgagg gttccatcca
      721 aatcgaaaaa aatcgctgtg atagaggtca tggtttctcc tatttgataa gcttattctc
      781 cgaaaatctc tttttggagg cgacgaccag taggggtggt agcgagtcca ccttcagctg
      841 tttcacgaaa ggcagttggc atgcttgctc ctacttggta catggcatcg atcacttcat
      901 ccacagggat tttagattcg atacctgcca aggccatgtc tgctgcgatg aaagcaaagc
      961 tagctcccat ggcattacgt ttgacacagg gaacttcgac caaacctgca acagggtcac
     1021 agatgaggcc tagcatattt ttaatgacaa aggcaatagc ttgactggcc tgataaggtg
     1081 ttccacctgc agccagagtc aaggcggcag cactcatagc agaggctgaa ccaacttcag
     1141 cttgacaccc accctcagca cctgagatgg aggcattgtt tgcgatgact agtccaaagg
     1201 caccagcagc aaagaggaaa tccaattgtt gctcgtggct gaggtctaat ttttcaatag
     1261 cagcagtgag aacggaaggg agacaaccgg cacttcctgc ggtaggagta gcgcagacca
     1321 agcccatttt agcattgtgt tcattgactg cgatagcatt tcgggcagca gagagaatcg
     1381 tgtaatctga taaagttttt tcgtttttga tgtagtgatc caatttggca gcatctccac
     1441 ctgtcaggcc actacgagat ttattttcat tgaggccaag ttggacagag gctttcataa
     1501 cttccagatt gcgttccatg agaaggaaga cttcttcacg ttcgcgaccg gtcaattcaa
     1561 actctgttgt aatcatgagt tctgcgacat ttccttgaaa gtccagatct gcttgctcga
     1621 ccaattcttt gatagaataa aacatgcttc ctcctattta aagaaattga cattgtggag
     1681 atgagggatt tttcgaattt cttcgatagc ctcatcacag ttgcgactgt caacttcgat
     1741 aatcataatg gctttttcac cagctttttc acgagtgaca ttcatctggg cgatattgat
     1801 accatagcgg gaaagcgcct ctgtaacaag ggcaatcata cctggaatat cttgatgaac
     1861 gatgatgata gtcggtgtat tcatattgag agagacggca aaaccattga gttcggttac
     1921 ctgaatattt cctccaccga tagaaatacc agtcacgctg atggtcttgt gggcattttt
     1981 aacagtaatt ttagtggtgt tagggtgagg ggcattgctg tctttctgaa tggtccagac
     2041 aatcttgata ccacgcttgt gggcaatttc cagactattt ggaatttcag gatcatctgt
     2101 atccattcct aaaatacctg caacaagggc taggtctgtt ccgtgaccac gataggtctt
     2161 ggcaaatgag ttaaaaagtt ggaattcaac ttctgtcgga gtatcatcaa aaatggaaga
     2221 gacaatcttc ccaatacgaa cagcaccagc ggtatggcta ctagatgggc caatcataac
     2281 tggtccgatg atatcaaaga cagattgaaa acgaagtgat ttcatcagtt tccccttata
     2341 aaaattctta tctctattat atcaaagaat gaggggcttg gctttaattg tggatgaaaa
     2401 cctttctaat acctcaaata gcataaaaat agtatctttt atgacaaaaa acaccttatt
     2461 tagggaaata aaaaataatt ttgtaatatt tctacataaa agtgtcaaga aacagtaata
     2521 tttaaagggt atgatagaac tatagaaaga aggagaattt tcgaatatga aatcaataac
     2581 taaaaagatt aaagcaactc ttgcaggagt agctgccttg tttgcagtat tttctccatc
     2641 atttgtatct gctcaagaat catcaactta cactgttaaa gaaggtgata cactttcaga
     2701 aatcgctgaa actcacaaca caacagttga aaaattggca gaaaacaacc acattgataa
     2761 cattcatttg atttatgttg atcaagagtt ggttatcgat ggccctgtag cgcctgttgc
     2821 aacaccagcg ccagctactt atgcggcacc agccgctcaa gatgaaactg tttcagctcc
     2881 agtagtaagt gaaacagttg tttcaactgt aagcggatct gaagcagaag ccaaagaatg
     2941 gatcgctcaa aaagaatcag gtggtagcta tacagctaca aatggacgtt atatcggacg
     3001 ttaccaatta acagattcat acctgaacgg tgactactca gctgaaaacc aagaacgtgt
     3061 agcagatgcc tacgttgcag gacgttacgg ttcatggact gctgctaaaa acttctggct
     3121 taacaatggc tggtattaag aactaacgaa caaataatat gtaaagagtc gaggaaatcc
     3181 ctcggctttt ttatctttcc ttcgcttggt aatagatctc tacttctttt ttgagatgag
     3241 acagcataga agtttcctcg gttagctcca gaagggagaa ccctttttgg ataagtttag
     3301 aatcgtctgt acaaatccca atacggacat agcagatagc cagcctatcg tagcttttct
     3361 tattcttggc gtcctccagt aaagtgtagc agatttgctt acacatgttt ttatcttgat
     3421 tgtatagata gagtgtggaa aggtttaata gaattgtcat tcgcaagtca tataaatgtt
     3481 gatagttttt atagacttct aggcgttgca atatttttcc agtgatgaga tggaggtatt
     3541 caatgggaaa actgaaaaga atggtattga ggatttttag atcactctca taccatgtat
     3601 cttgtttttc aatttttttc caaagttttt tgacaacctg ttctgcgtgt tttgatagtt
     3661 cccctgcccc gtgttgacgg agataaataa caacttcaag catatctctg atttcttcta
     3721 tagggagatc gtggtgggtc ttgaggtagt cttggcattt ttgaaataaa ttgacaagat
     3781 cgctggtccc gataatggaa cgcatattga gataggtttg cataatctct gtgcgttgac
     3841 ttggttgata aagttgacag atatattcaa actcttcaaa actcatattg atttgacgga
     3901 gaagaaattc catattttca tattttgggg ttgtcttgcc actttcgatt tttgataagc
     3961 tggttcttga gagaacaccc ccacagacct cttcttgggt cagccccttt gactcacgta
     4021 tttctttata gacctttccg aaatcataac gcatgatttt ctcctttctg tgaaaaaagt
     4081 taacaaacaa taaaattttg ttaaaaatat tgtatcatag caatgtaaaa agtaaaaaag
     4141 aaagtcgaaa taaatttgtg aaaaaagtta acgtaatttt aaaatgagct taataagagg
     4201 tatgatgtag gtagaaaata aagataggag aaaaagtatg aataaaaaaa tattttgtat
     4261 tctagtatgt attatatcat tgattagtct ggccataatc tttccatggg gctggtcgat
     4321 ataatgtaag ggagcttgag ttgttaaagg aggcttagtc cttggacaaa aatctgctgt
     4381 actacaatgc ttggaaagat atcaagaaca aagaatgata cgattgaaaa taaactgatt
     4441 caagaactac tgggcagtga ttgaggtttg ggtgacatca aagaaaagtt agattaattc
     4501 taaagaattg agtatttcta tgttaaaaaa atatccttgt acgatgcaac atgatcagtc
     4561 agattgtgct gcggcagttg tttcaacagt tcttttatct tacaaaaagg aactatcaat
     4621 catgaagatt cgggaaatta ttggtacaga catgtatgga acgactgtca gtggtattgt
     4681 ttcaggtctg aataagttga attttacagt aaaagctgtt cgagtagcac tggaagattt
     4741 gactccaaaa ttaacatttc ctgcgattct tcaagttaag aatgatttag gtcaaaatca
     4801 ttttgtggta ttacatagta taaaggagaa aataaatgga actcgtatta ccaaataatt
     4861 atgttgtgat tgatgaagaa gagatgatgt accttgatgg gggggctata tatataccca
     4921 ggtgggcaat tacaggagcc attactggtg cagcatatgc agcattagca gcagcaggag
     4981 gtggaggcct tcaactagtt cttgcatctt atggattacg ctccgcactg gtagctggga
     5041 ttgttaaagg tttaggagta ttaggaattc atattggaaa tgcttttgca aatactgtta
     5101 ttagaagtat tgcatctgct ggaattggtg ctggagctga ttggatttta ccaatattat
     5161 tgatggctgg gatgggcgac gtgataatca attgagaata ggataatagt attatgatgg
     5221 ttgttttaat ttcagttatt gttgcattcc tgtaccataa aattacagag tatgtcaaca
     5281 acaggaaaat tgatattgtt atttttctat cattgctgat ttctcatttt atttggaatt
     5341 actatttttt accaattgaa atattgatag caatcagtgg agcaaattta ttattaagtt
     5401 ttttaaaaaa attacagatt aaataatatt attccatatg acatatcaac taggtcatta
     5461 gattttattg aagatataaa ttaaaaacga ggttgggaaa aaactcaatt tgaaagtgaa
     5521 attgagtaaa tttttctatt atataacgca tattatcaag atttttgaac acctgataat
     5581 atgcactttt cttattttaa agattttttg cctttcttca tttcttgaat gaattggaga
     5641 gatgacttta tttaggtgtt tgacattact atagatagga tttagcagta ctaatatgaa
     5701 ctcatattat aggtgcagac ttaaattttg gaagcaagct aaaggatatc ttatctaggt
     5761 aactaagtgc tataaacaaa gtcaacgatc acttgttagg aaacgatcct aactatgctt
     5821 aataaaaaag gagaaaataa atggaactcg tattaccaaa taattatgtt gttcttgagc
     5881 aagaagagat gatgtatctt gatgggggtg gtgttggttg taactggtgg aacaaacgtg
     5941 ataatattgc tgcagctgca gtttctggag ggatgtcagt ataccagaca tatgccatta
     6001 aaaagttaat tgctaatcgt ggtatgatga gccgagtcgt tagaagtgct ataacaagat
     6061 atattggtgg agttagtatg gcaactatta ctaatgctct taatattgcg gctactgttg
     6121 ctgaagtttt ctcattagga ggagccattg catatggttt agatatcgta gacggtaaat
     6181 ttgatgg
//
LOCUS       DAVBCV010000087         5939 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBCV010000087 DAVBCV010000000
VERSION     DAVBCV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.087"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            390..1010
                     /locus_tag="VQ516_001992"
     CDS             390..1010
                     /locus_tag="VQ516_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512379.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1096..1539
                     /gene="tsaE"
                     /locus_tag="VQ516_001993"
     CDS             1096..1539
                     /gene="tsaE"
                     /locus_tag="VQ516_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2512380.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            1529..2047
                     /locus_tag="VQ516_001994"
     CDS             1529..2047
                     /locus_tag="VQ516_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2512381.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2055..3071
                     /locus_tag="VQ516_001995"
     CDS             2055..3071
                     /locus_tag="VQ516_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2512382.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEKALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3155..4411
                     /locus_tag="VQ516_001996"
     CDS             3155..4411
                     /locus_tag="VQ516_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2512383.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNLLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQVDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGISSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            4466..5632
                     /gene="recA"
                     /locus_tag="VQ516_001997"
     CDS             4466..5632
                     /gene="recA"
                     /locus_tag="VQ516_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2512384.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
BASE COUNT         1843 a          977 c         1375 g         1744 t
ORIGIN      
        1 tgtttagtta atcttgacat tatagttttt ctgaaacgtt aaataacctg taaggctgat
       61 agttagatta atactatcag ccttataatt atgtgactct gagtccgtct cgctccgtga
      121 ggtgcgagac aaaacaacca cccgtttgac ggattttcat gatttcaaat ttatattctt
      181 cgaaaatctc ttcaaatcac gtcagctcta tctgcaatct caaaacagtg ttttgagcaa
      241 cctgtggcta gctacctagt ttgctctttg attttcattg agtattagtc tctagtgttt
      301 gtttagcgtc ttaaagagac atttgagaag attagaaaca gttctctctt ttctcctagt
      361 ataatgattg gtaggaaggg agagagaaga tgaaatcaat gagaatctta tttttgttag
      421 ctttaattca aatcagtttg agtagctgtt tcctatggaa ggaatgcatc ttgtccttta
      481 aacaaagtac agcttttttc atcgggagca tggttttcgt ttcaggaatc tgtgctggag
      541 taaattatct ttatacccgt aagcaagaag tccatagtgt cctagccagt aagaagtcgg
      601 tgaagctttt ttacagtatg ttactcttaa ttaatttgtt aggagctgtt cttgttttgt
      661 cagataactt gttcatcaaa aatacgctgc agcaagaatt agttgacttt ttattgccat
      721 ccttcttttt cctatttggg ctagatttgc tgattttttt acccttgaaa aaatacgtgc
      781 gcgattttct tgctatgcta gacagaaaaa agacagtgtt ggtgactatt ttagcaacac
      841 ttcttttctt aagaaatcca atgaccattg tctcacttct gatttatatt ggactgggct
      901 tgttttttgc agcctatctt gtcccaaatt cggttaagaa ggaagtttcc ttttatggtc
      961 atattttccg agatcttgta ttggtcattg ttacgctcat tttcttttag agaaagcctg
     1021 ttttttatgg atgttatgat gataagttta gtaggaaaat agacacaaaa gaaaagtttt
     1081 tggtataata agagtatgta cacaaaaaat gaagaagagt tgcaagcctt aggggagcgt
     1141 ttgggccatc tattagcaaa gaatgatgtt ttaatcttaa ctggagaact gggtgcaggt
     1201 aaaacgacct ttactaaagg acttgcaaaa ggattacaga tttctcaaat gattaaaagt
     1261 cccacctata ctatcgtgag agagtatgaa ggtcgacttc cactttatca cctagatgtt
     1321 tatcgtattg aaggagatgc tgattctatc gacttggatg agtttatctt tggtggcggc
     1381 gtgactgtta ttgagtgggg aaatctctta ggagatgcct tgccagatgc ttatttagaa
     1441 ttggaaattc taaaagaagc agatggacgc cgtttaaatt ttcaggcaaa gggtttgcgt
     1501 gctgagaaat tgttagagga gcttcaatat ggagtatgaa ttgctcatta gggaagcaga
     1561 gcccaaagat gcagctgaat tagtggcctt tttaaatcgt gtgagtttgg agacagactt
     1621 taccagccta gacggagatg gtattctctt gactagtgag gagatggaaa tattcctcaa
     1681 caagcaagct agttcggaca atcagataac cttacttgca ttcttaaatg gcaaaattgc
     1741 tggtattgta aatattacag ctgatcagcg taagagagtc cgtcatattg gagatctctt
     1801 cattgtgatt ggaaaaagat attggaataa tggcttggga agtttgttgc tagaagaagc
     1861 gatagagtgg gcacaagcaa gtggcattct gcgtcgtctc caactgactg tccaaactcg
     1921 taatcaagca gcagtccatc tttatcaaaa gcatggcttt gtcattgaag gtagccaaga
     1981 gcgtggggca tatatagaag aagggaaatt tatcgatgtt tacttgatgg gtaaactgat
     2041 aggttagtag aaatatggtt aaaaaaatta ttggaatggt gctagcttta ctttctgtaa
     2101 ctgtagtagg agtaggtgtt tttgcttata ctatttatca acaagggaca gaaaccttag
     2161 ctaaaaccta taaaaaaatc ggcgaagaaa ccaaggttat tgaagcgact gaacctctaa
     2221 ccattctgtt aatgggagtg gacaccggaa atgttgaacg aactgaaact tgggtcggta
     2281 gaagtgatag catgatcttg atgacagtga atcctaaaac gaaaaaaaca acaatgatga
     2341 gtttagagcg ggatattctg acgcgcattg aatcagggaa tggtcaggct catgaagcga
     2401 aactgaactc agcatatgca gatggtggag cagagcttgc tatagaaacc attcaaaaaa
     2461 tgatgaatat ccatattgat cgctatgtga tggtcaatat gagagggttg caaaaactag
     2521 tggatgcagt aggaggtatt acagtcaata atatcctagg tttcccaatt tctatcagtg
     2581 accaagaaga atttaatact atttctatcg gtgttgggga gcaacatatt gggggagaaa
     2641 aagccctagt ctatgcacga atgcgttacc aagatcctga gggggattat ggtcgtcaaa
     2701 aacgtcaacg tgaagttatt caaaaagtca tggaaaaagc tctcagttta aatagcgttg
     2761 gtcattatca agagattcta aaagctttga gtgacaatat gcagaccaat attgatttgt
     2821 ctgcaaaaag tatccctaac ttgctaggct ataaagattc atttaaaacc attgaaactc
     2881 agcagttgca gggtgaagga gagatacttc aaggtgtttc ttaccagatt gtttcaagag
     2941 cacatatgtt ggaaatgcaa aatatactcc gacgttcttt gggacaagaa gaagttactc
     3001 agcttgaaac caatgcggtt ttatttgaag atttatttgg cagagcacct gttggtgatg
     3061 aagataatta acttttgata caaataaaaa atcaatcgta ggaaaattcc tgcgattttt
     3121 tcaaaaaaat acgaatagat aggtaggagg aaacatgaaa gcagaaatca ttgctgttgg
     3181 aacagagatt ttgacaggac agattgtcaa caccaatgcc cagtttttgt cggaaaaact
     3241 agctgagatt ggggtagatg tatattttca gacggctgta ggagacaatg aagttcgtct
     3301 cttgtctttg cttgagattg ccagtcaacg tagcagtctg gtgattttga caggcggttt
     3361 ggggccaact gaggacgacc taaccaaaca aaccctagct aaatttttag ggaaagcatt
     3421 agtctttgat cctcaggctc aggagaagtt ggatatcttt tttgccctgc gaccagacta
     3481 tgcccgaaca ccgaataacg aaagacaagc tcaaattgta gaaggagcga ttccactgcc
     3541 aaacgaaaca ggactggctg ttggaggaaa attagaagta gacggagtga cctatgtcgt
     3601 ccttccaggt ccgccaagtg aattgaaacc catggtctta aacctacttc tacccaagtt
     3661 gatgacaggg agcaagctgt attcccgagt tcttcgtttc tttgggattg gcgagagcca
     3721 gctggttacg attttggctg atttaattga taatcaggtc gatcctacct tggcccctta
     3781 tgccaagaca ggagaagtca ctctacgtct gtcaacaaag gctagcagtc aagaagaggc
     3841 gaatcaagcg ctggatatct tggaaaatca aatcttggac tgccagactt tcgaaggaat
     3901 ttctttacga gacttttgct atggttatgg ggaagaaact agtttagcca gcattgtggt
     3961 agaagaactg aaaaggcaag ggaaaaccat cgcggctgca gagagtttga cggcaggtct
     4021 tttccaagct accgtggcga atttttctgg aatttcaagt atatttaagg gtggttttgt
     4081 gacctatagc ttggaggaaa aatcaaggat gttggatatt cctgccaaga atttggaaga
     4141 acatggtgtg gtgtctgaat ttacagctca gaagatggct gagcaggcac gaagcaagac
     4201 ccagtctgat tttggaatta gtttgactgg agtggcagga ccagatagcc tagaaggaca
     4261 cccagttggg acagtcttca taggcttggc gcaagatcaa ggaactgagg ttatcaaggt
     4321 taatattgga ggcagaagcc gagcagatgt acgtcacatt gcggttatgc atgcctttaa
     4381 cctagttcgc aaggctttat taagtgacta atttttgata taatagtaga taggtctgag
     4441 gatcattaga atgtaggaga atagaatggc gaaaaaacca aaaaaattag aagaaatttc
     4501 aaaaaaattt ggggcagaac gtgaaaaggc cttgaatgac gctcttaaat tgattgagaa
     4561 agactttggt aaaggatcaa tcatgcgttt gggtgaacgt gcggagcaaa aggtgcaagt
     4621 gatgagctca ggttctttag ctcttgacat tgcccttggc tcaggtggtt atcctaaggg
     4681 acgtatcatc gaaatctatg gcccagagtc atctggtaag acaacggttg cccttcatgc
     4741 agttgcacaa gcgcaaaaag aaggtgggat tgctgctttt atcgatgcgg aacatgccct
     4801 tgatccagct tatgctgcgg cccttggtgt caatattgac gaattgctct tgtctcaacc
     4861 agactcagga gagcaaggtc ttgagattgc gggaaaattg attgactcag gtgcagttga
     4921 tcttgtcgta gtcgactcag ttgctgccct tgttcctcgt gcggaaattg atggagatat
     4981 cggagatagc catgttggtt tgcaggctcg tatgatgagc caggccatgc gtaaacttgg
     5041 cgcctctatc aataaaacca aaacaattgc catttttatc aaccaattgc gtgaaaaagt
     5101 tggagtgatg tttggaaatc cagaaacaac accgggcgga cgtgctttga aattctatgc
     5161 ttcagtccgc ttggatgttc gtggtaatac acaaattaag ggaactggtg accaaaaaga
     5221 aaccaatgtc ggtaaagaaa ctaagattaa ggttgtaaaa aataaggtag ctccaccgtt
     5281 taaggaagcc gtagttgaaa ttatgtacgg agaaggaatt tctaagactg gtgagctttt
     5341 gaagattgca agcgatttgg atattatcaa aaaagcaggg gcttggtatt cttacaaaga
     5401 tgaaaaaatt gggcaaggtt ctgagaatgc taagaaatac ttggcagagc acccagaaat
     5461 ctttgatgaa attgataagc aagtccgttc taaatttggc ttgattgatg gagaagaagt
     5521 ttcagaacaa gatactgaaa acaaaaaaga tgagccaaag aaagaagaag cagtgaatga
     5581 agaagttccg cttgacttag gcgatgaact tgaaatcgaa attgaagaat aagctgttaa
     5641 agcagtggag aaatccgcta ctttttcgat ttttgattca agtttttaga ttatatatag
     5701 tagcttgaaa taagatatga acaactctat taggaaagtc aaattaattt ctagaaatgt
     5761 tttagcagct acagcgtact attccaaact caaccaacta taatagatcg aaactagaat
     5821 agtacatatc tacttctaaa acattgttaa aaatcgattt gactttcctt atttcattcc
     5881 gctatatata agtttgctgt ttcttgtcgc tcctctggaa agctgatata atagcttta
//
LOCUS       DAVBCV010000088         5470 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBCV010000088 DAVBCV010000000
VERSION     DAVBCV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.088"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            135..1628
                     /locus_tag="VQ516_001998"
     CDS             135..1628
                     /locus_tag="VQ516_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2512385.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1641..2687
                     /locus_tag="VQ516_001999"
     CDS             1641..2687
                     /locus_tag="VQ516_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2512386.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIY
                     GSGGIMGYAKDWIVKKNSVIIGRKGNINKPILVRENFWNVDTAFGLEPVLEKINSEYL
                     FYFCQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADFVALVDKSQFACEI
                     AIKVWRNSLKFSII"
     gene            2717..5413
                     /locus_tag="VQ516_002000"
                     /pseudo
     CDS             2717..5413
                     /locus_tag="VQ516_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1815 a          845 c         1140 g         1670 t
ORIGIN      
        1 gacgtggttt gaagagattt tcgaagagta taaaatattc aggtgacgca tagatatagt
       61 taattgaagc tttgtttgaa atctgataaa ataatgatat tactaagttt taaaaactaa
      121 agaaaaggga agatatgatt acaggcgaat taaaaaataa aatcgatcag ctgtgggaaa
      181 ttctttggac agaaggaaac gcaaatcctt taacaaatat tgaacagttg acttatctct
      241 tatttatgaa agatttggat agtgtcgagc ttggacgtga aagtgatgct gaatttctag
      301 ggattcctta tgagggagtt tttccaaaag ataaacctga ataccgttgg tcaactttta
      361 aaaatatagg agatgctcag gaagtttatc gtttaatgac tcaggagatt tttccgttta
      421 ttaaaaatct caagggggat acagatgata cagccttttc acgatatatg cgagaagcta
      481 tttttcaaat aaataaacct gctacgcttc aaaaggcaat ttctatctta gatgtttttc
      541 caactagggg attagatgta gattttgata atgacaaaca aagtattact gatatcggag
      601 atatctatga atatctgtta tcaaaattgt cgaccgcagg taaaaatgga cagttccgta
      661 cacctcgtca catcatcgat atgatggttg agttgatgca accgactatc aaagatatca
      721 tctcagatcc cgctatgggt tctgctggct tcttagtatc tgctagccgt tacttaaagc
      781 gtaagaaaga tgaatgggaa accaatacag ataatatcaa tcattttcat aatcagatgt
      841 ttcatggaaa tgatacggat acgactatgt tgagacttgg ggcgatgaac atgatgctac
      901 atggagtaga aaatccacaa atcagttacc ttgactcgct gtctcaagat aatgaagaag
      961 ccgataaata tactttggtt ttagcaaatc ctccttttaa gggctcactt gactacaatt
     1021 caacctctaa tgaccttctt gcaaccgtaa aaaccaaaaa aacagaatta ctctttcttt
     1081 ctcttttctt gcgaacttta aaaccaggtg gacgagcagc agttatcgta cctgatggtg
     1141 tcctttttgg ttcgtctaaa gctcataaag gaattcgtca ggaaattgta gagaatcata
     1201 agcttgatgc tgtaatctca atgcctagtg gtgtgttcaa gccttatgct ggagtttcaa
     1261 ctgccattct catctttaca aaaactggta atggtggtac tgacaaagtc tggttttacg
     1321 atatgaaagc ggatggttta agtttggatg ataagcgaca accgattagc gacaatgata
     1381 ttccagatat tatcgaacgc tttcatcatc ttgaaaaaga agcagaacgt cagagaacgg
     1441 atcaatcttt ctttgttcca gttgctgaga taaaggaaaa tgattatgat ttgtctatca
     1501 ataaatataa agagattgag tatgaaaaag ttgagtatga accaacagag gtcatcttaa
     1561 agaaaatcaa tgatttagaa aaagaaattc aagctggctt ggctgaatta gaaaaattac
     1621 tcaagtagag aggtggctgt atgaaaaaag tgaagttggg ggaagtctta tctctaaaaa
     1681 aaggcaagaa agccactgta cttgctgaac aaacaactct aagccaacgt tatattcaaa
     1741 tagatgattt aagaaataat aataatttaa aattcactga aagtttaaat atgactgaag
     1801 cactcccaga tgatattctg atagcatggg atggagctaa tgcaggaaca gttggttatg
     1861 gattatcggg agctgttggt agtacaatta cggtcttaaa aaagaatgag cgatacaaag
     1921 aaaaaattat atcagattac ttgggagtct ttttggaaag taaatcgcag tatttacgag
     1981 aacattcaac aggtgcaaca attcctcatt taaacaagaa tatattactt gatttacaat
     2041 tagaattgct aggtatcgaa gaacaagaga acattatctg tattcttaat acgattaaaa
     2101 ggcttattac taaaagaaaa ttgcagttag atgaactaaa cttgctcgtc aaatcccgat
     2161 ttaacgagat gtttggggat gttattctaa atgaaaaaga atggaaagtt agcaaatgga
     2221 acgagattct aacaataaga aatggtaaaa atcaaaaaca ggttgaagat gctgatggga
     2281 aatttcctat ttatggaagt gggggaatta tgggatatgc taaagattgg atagtgaaga
     2341 aaaattcagt tattattgga cgtaaaggaa atataaataa acctatttta gttagagaaa
     2401 atttttggaa tgtagataca gcatttggat tagagcctgt tttagaaaaa ataaactcag
     2461 aatatttatt ctatttttgt caattataca attttgagaa gttaaataag gctgtaacaa
     2521 tacctagttt aactaaatca gatttattaa atatttccat ccccctcccc ccactagccc
     2581 tccaaaatga gttcgcagac tttgtagccc tggtcgacaa atcacaattt gcttgtgaga
     2641 tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta aactatttgt
     2701 ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg aatatgaatc
     2761 tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg tggctactgc
     2821 ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc acgattcata
     2881 tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg attttaggga
     2941 tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg gaaaccatgc
     3001 tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc atttgtatca
     3061 gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt attttgatga
     3121 gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat ctatgataaa
     3181 gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg ctcagtccgt
     3241 agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc aagatgtgcc
     3301 tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg atatcgatct
     3361 ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg ttgatggtct
     3421 caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa atgggaaaat
     3481 tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg aagtacaggt
     3541 caaagaatat gctgaagctt tggagaaaca tatcggctat cagccaattt gctttattac
     3601 aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg caggctttta
     3661 ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac cgcttgagga
     3721 tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac atgccattgc
     3781 aagcgtttgt gaagctttct ctgatcatcg tagacaggca cttttggtta tggcaactgg
     3841 ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc ataactgggt
     3901 aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcat atgattcgtt
     3961 tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag aaggagctca
     4021 atcaagtcga atggtctttt caacttatcc gaccatgatt ggagcgatta gtggtcaaga
     4081 agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa ttgacgaatc
     4141 tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg caaggattgt
     4201 aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat tctttaattt
     4261 ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag acggatattt
     4321 agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc tacattatga
     4381 tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg atagctgtga
     4441 aaaagatatt gatgggagtg tgtttaattc ctttattttc aataaaagta cagtagaaat
     4501 tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg aaattggtaa
     4561 aactattatt tttgctaaaa atcatgacca tgcggaatat atcagaggta tttttaacaa
     4621 ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta ttaagcatta
     4681 tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg cgatttctgt
     4741 cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct tcttcaagaa
     4801 agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc tatgtaaaga
     4861 tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg gggacaattt
     4921 tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt cgctgactca
     4981 gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc aataccaaga
     5041 agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag gtcgtataga
     5101 gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt atagctatag
     5161 gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc aaaaaaatct
     5221 ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat ttgatttgtg
     5281 gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc atatttccca
     5341 agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg tttttgagca
     5401 ggctgaaatt atggctcttt gtcaactgta gtgggttgaa aaaaagctaa gcttgagaaa
     5461 ggacaaattt
//
LOCUS       DAVBCV010000089         4780 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBCV010000089 DAVBCV010000000
VERSION     DAVBCV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.089"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(1..157)
                     /locus_tag="VQ516_002001"
                     /pseudo
     CDS             complement(1..157)
                     /locus_tag="VQ516_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(541..1737)
                     /gene="tuf"
                     /locus_tag="VQ516_002002"
     CDS             complement(541..1737)
                     /gene="tuf"
                     /locus_tag="VQ516_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2512387.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2648..3517)
                     /locus_tag="VQ516_002003"
     CDS             complement(2648..3517)
                     /locus_tag="VQ516_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2512388.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3740..4420)
                     /locus_tag="VQ516_002004"
     CDS             complement(3740..4420)
                     /locus_tag="VQ516_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2512389.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDK
                     PTPEPGTPKAETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGF
                     FKKKEDEK"
     gene            complement(4378..>4780)
                     /locus_tag="VQ516_002005"
     CDS             complement(4378..>4780)
                     /locus_tag="VQ516_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2512390.1"
                     /translation="FKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYKE
                     GSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANAY
                     QKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
BASE COUNT         1342 a          950 c          931 g         1557 t
ORIGIN      
        1 attgtttgat tacagtctaa aagttctata atctctttat aagatttgcc catatcagac
       61 gaaatagaac gatttgaaga cgtttatgat atttagctgt acgagagtct tttaaaagtg
      121 ttttgatggt ttggatttct tctttaattg atttcatatt actattgtat aatgcttttt
      181 gattttagtc tagtattata ctcttcgaaa atctcttcaa accgcgtcag cttcgccttg
      241 tcgtatagat gttactgact tcgtcagttc tatctacaac ctaaaaacag tgttttgagc
      301 agtctgtagc taactttcta tagtaaaatg aaataagaac aagacaaatc gatcaggaca
      361 gtcaaatcga tttctaacaa tgttttagaa gtaaatgtgt actattctag tttcaatata
      421 ttatagtttg ctctttgatt ttcattgagt attagttcaa tatctaaaaa aagaaccttg
      481 ccaagcaaga ttcttttagt gtctgactta aataattgtt cttctgggaa ctaaatcgaa
      541 ttaagcttcg atttctgtaa ccatacctga accaacagta cgtccaccct cacggataga
      601 gaatgtagta ccttgttcta cggcgattgg gtgaatcaac tcaacgtcga ttgtcacgtt
      661 atcaccaggc attaccattt cagtacctgc tggaagttcg attgaacctg taacgtcagt
      721 agtacggaag tagaattgtg gacggtagtt gttgaagaat ggagtgtgac gtccaccttc
      781 ttctttagta aggatgtaga cttcaccttt gaatttagtg tgtgggttga ttgaacctgg
      841 tttagcgata acttgtccac gttcgatttc atcacgttga acaccacgaa gaaggacacc
      901 tacgttatct ccagcaagac cttcgtcaag ttgtttacgg aacatttcaa caccagtaac
      961 aactgctttt tgagtttctt ctttgatacc aacgatttcg atttcgtcgt tgactttaac
     1021 gataccacgg tcgatacgtc ctgaagcaac tgtaccacgt ccagtgattg agaatacgtc
     1081 ctcgactgga agaagcaatg gtttgtcagt gtcacgttct ggttctggga tatactcatc
     1141 aactgtgttc atcaattcca taacgatgtc ttcgtattta gagtcacctt caagagcttt
     1201 aagtgctgaa ccttggataa ctggaagatc gtcacctggg aagtcgtatt ctgacaatag
     1261 gtcacggatt tccatttcaa ccaattcaag caattcttcg tcgtcaacca agtcaacttt
     1321 gttcatgaag acgataaggt gtttaacacc aacctgacgt gaaagaagga tgtgctcacg
     1381 agtttgtggc attggtccgt cagttgaagc tactacaagg atagctccgt ccatttgagc
     1441 agcaccagtg atcatgtttt taacgtagtc cgcgtgtcct ggagcgtcga tgtgagcgta
     1501 gtgacgtttt tcagtttcgt actcaacgtg cgcagtgttg atagtgatac cgcgttcgcg
     1561 ttcttctgga gcagcatcga tagacgcata gtctttaggt tggttaactg atgaaggcaa
     1621 gcgacgtgcc aaaacagttg tgatagctgc agttagggta gttttaccgt ggtcaacgtg
     1681 tccgatagta ccaatgttaa cgtgtggttt actacgatcg tatttttctt ttgccatttg
     1741 agtaaaagcc tccaataaaa tatattttat agatagacag taggcaatac agtctaactt
     1801 tccttactat tttatcaaat ttcagctaaa ttgcaagtgt tttacaacgt ttttgtgaat
     1861 tattcttggt aaaaatttaa ctaattaata gaaagtacta aatagactta tccttcaagg
     1921 agttttcagc attatccatc cactcacttg ctgaaaatgt aaacagtctc tggtttatat
     1981 agtaaaatga aacaagaata gagcaaatcg atcaggacag tcaaatcgat ttctaacaat
     2041 gttttagaag tagatgtgta ctattctagt ttcaatctac tatacaacct caaaactgtg
     2101 ttttgaacaa cctgcggcta gcttcttagt ttgccctttg attttcattg agtataaaat
     2161 tgcagataaa acgataacaa tcataggact cagcagagtc aatgtaggtc gaagaatagc
     2221 ccccctaatc gcttatttta tagcaaaatg aaatagaaac tgaacatttt gttgaggaag
     2281 gtaaatcaat tcctcacaat gtttgagaaa gaagggagga ctattccaac ttcaacccac
     2341 tataaagcag acttacaaga tagggaaccc atctccgata atatatagta gaatgaaaac
     2401 tgcacaattt gattggagaa ttcaaaccaa tttctaacaa tgttttagaa acaagggtct
     2461 attatttcaa ctgcaaccta ctatagccca ttgaagctgg aatagcccac tgagacttct
     2521 aaaccactgc tggagaggag tttgtttaga gtttctttca tttccctaaa aaatcaaaaa
     2581 gagagaacca gtctgatcct ctcttactta tagatggtgc tcactcaaat gttaaaggag
     2641 ctatttctta gagataaagg aatttgaata cagctactgc cgcaattgct gctgcgatag
     2701 gtgctactac tggtacccaa gaataccacc attttgaatc gcctttatgc tcaccaagaa
     2761 ctgattttgg aaggaaagca tgaaggagac gtggtcccaa gtcacgggct gggttcaagg
     2821 caggtcctgt aggtcctcca agtgatgtta ccaaagccat aacgaggaat ccaagtgcca
     2881 agtgagccac agaaagtcct gaagcagtgt gtggagccac ctgtgcttta atagccaagt
     2941 cagaaaaatc aactgtttgt cctgcttcta ttgccttttg tttcatgaat tgaagcactt
     3001 cagcaccaaa gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca aatgaaccaa
     3061 caaactcatt aatcaaacca ttgacagttg ctgcatagcg actttctttt gtaccatggt
     3121 caatacttga aatagttgag aaagttccca agatgttatt tggtttttca gttttcaagt
     3181 agaatggacg gtatgttgcc acaactaagg cttggccaaa gatagccccc aagacttgcg
     3241 cgataatgta aggtaccact tgtgcccaag ggaaaagacc gctaactgca agccctagag
     3301 tgaaagcagg gttgatgtga ttcccagata cgttaccaaa catcaaggct gggatcataa
     3361 cccccatacc ataaccaaca gcgatgacga tccagccact ttggtgacct ttcgtacctt
     3421 taagttcaac gttggcaact gcaccattcc caagaatgat caaaatggca gttcccaaaa
     3481 attcagtggc atacttcagt gcccatgtga aatccatttg ctagattctc cttaaaattt
     3541 tttagatatt ccttatttta tcaattttta ccctatttag caactgattt ctggaaatat
     3601 ttttaaaaaa ataggattca tagtttccca gttcaacgta cttgtaagcg tttttattct
     3661 tcacgaaaga cctcattctc aaaaccagga aaaagcccac atctgtgatc ttttcctggt
     3721 tcctagattc taaaatctat tatttttcat cttctttttt cttgaagaat cctaatccta
     3781 gaccagctaa caaggtcatg ataccagcac tagccaaggt agcattagct tctgtacctg
     3841 tgtttggcaa ttcctctctc ttacctgtct cataagtcgg aacttctggg tctggattca
     3901 ctggagtttc agcttttgga gtacctggtt ctggagttgg tttatctggt gttggagtct
     3961 cttgatttgg tagttctggg tttggtgtcg gaacacttgg atgctctgga tttggttgtg
     4021 ggattggttt ttctggagtc ggttcacctg gagtacctgg ttcctgagga cttggaattt
     4081 ctggtttttc tggagttggt ttatctggtg ttgataaacg gtcatacctt accgttattt
     4141 ctttatcact agagtctgac gtaacttctt gtgattcaac tgttggaata tctggatctt
     4201 tgtacttgtc aatcttacca gatataacct cgtcccagtt tcctgttgtc cattcaccgt
     4261 aggttacaac tcccgtgacc ttgttctcag tttttgtacg gcttaaggtt acaggttgaa
     4321 caacatcttc ttttacattt tggttcgtaa ctttatcaac gtaatgaatg atacgcgtta
     4381 tagtcttcgt ctcagtagag gttgctgttt tgggaaccac tgtttcctca acattctcac
     4441 ggtagtaata gtcaactgtt gcaccgtctt ctggtacgca tttgcaggag ttgctaccaa
     4501 ggtgtatgtt gttttccttg tgataactcg gtcttctttg tcctcagttg ttgttttccc
     4561 ttcaatagtt tttgattctg tggtatactc agaacctatc gctaaatcag cttttataac
     4621 agactctgcc aacttctctt ggctaccttc tttatagtaa ttggctgtta ctgtagcagt
     4681 ggttggcgct tcgctttctc tataaactaa ggtcactgtt ctaccttcgc ttacaatatt
     4741 cccagttaaa cttgcagaat ttgtatctgc ttctttaaaa
//
LOCUS       DAVBCV010000090         4723 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBCV010000090 DAVBCV010000000
VERSION     DAVBCV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.090"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..141
                     /locus_tag="VQ516_002006"
     CDS             <1..141
                     /locus_tag="VQ516_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512391.1"
                     /translation="LLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSC
                     FN"
     gene            complement(134..307)
                     /locus_tag="VQ516_002007"
     CDS             complement(134..307)
                     /locus_tag="VQ516_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2512392.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(465..2072)
                     /locus_tag="VQ516_002008"
     CDS             complement(465..2072)
                     /locus_tag="VQ516_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2512393.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2225..3709
                     /locus_tag="VQ516_002009"
     CDS             2225..3709
                     /locus_tag="VQ516_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2512394.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3804..4286
                     /locus_tag="VQ516_002010"
     CDS             3804..4286
                     /locus_tag="VQ516_002010"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2512395.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(4572..4723)
                     /locus_tag="VQ516_002011"
                     /pseudo
     CDS             complement(4572..4723)
                     /locus_tag="VQ516_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1360 a         1093 c          885 g         1385 t
ORIGIN      
        1 cttttacctc ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat
       61 atcaaaaagc acctcaaaaa ggtattacca agttgcaata ccttttatga ggctcttttg
      121 tcttgttctt gtttcaattg actatattat cagtgtaata gtctaataga tagttaaatg
      181 tagttggatt tccactaaag gttgatgagt aagtttttgt atctgaacct gattggccgc
      241 aagcagctaa aagcaaaaca gatgcaaaag tcaggcctgc actaaggaca cgcttcttta
      301 tgttcatctt ctttctcctt aatagtggga atttgtaaag ttaattgaat ttcgagaatg
      361 aaagtttata aaccttggtt ataaaaaaca aaggatttct gcttttatac agtcctccct
      421 tgtttttata tgatttcaat tttaaatttt tctgcaaaaa atatttatag taattccaca
      481 cagaaagcat cccatggaac taagatttgt ttttcaaaga cttcttgagc tagggtgttt
      541 tcaatcaaga cagatttgac ttttccttct actgtcaagt cttgctcttc attggacaag
      601 ttagccacaa ctaggaagcg acggtcgcca tccttacgta tataagcaaa gaccttatca
      661 gccgtatcaa gcaattcaaa gtcagctcga actagccagc tattctcctt gcgaatttgg
      721 accagtttct gataggtata gaaaatagaa tctggatttg ccagcgcttc ttggacattg
      781 atcatctcgt aatttggatt aaccgccaac caaggttgac ctgttgagaa accagcgttt
      841 ttgctctcgt cccattgcat aggggtacgg gcattgtcac gtccaataac acggatactg
      901 tccatgattt cttcaatcgg aacacctttt tcaagagcct cacgcgcata gttgagagat
      961 tcaatatctt ctacttgatc cagtgtttca aacggatagt tggtcatccc aatctcctca
     1021 ccttggtaga tataaggagt tcctctcata agatgaagca agattgcaaa ggctttggca
     1081 gatttttcgc ggtattcttg gtcatttccc cagattgaga caatacgagg gaggtcatgg
     1141 ttgttccaga agagggaatt ccagccgtcc tcaactccta actctgtctg ccatttgttg
     1201 aagatttctt ttaacttagc gatattcagc tctttttgat agtgccattt aggctgacct
     1261 tcctgatact gaagaccgat atgttcaaac tggaagacca tagacaattc ttgccccttt
     1321 ggatcagagt agagcttggc aatctctggc gttgctcccc aagtctcccc tactgtcaag
     1381 agatccttat ctccaaaggt cgcctgattc atttccttga gatagggatg gagcatagga
     1441 ccattattga ctaccttctc gtcaggaatt ttgccaatca tgtcaataac atccatacgg
     1501 aaaccaccaa tacccttatc aatccagaag ttcatcatct cataaatttt ctggcgaagt
     1561 ttttcatttt cccagttgag atccggctgt ttcttgctga aaaagtggag atagtattga
     1621 cctgactttt catcgtattc ccaagcagac ccactaaaga tagaatctaa atcgttaggc
     1681 tcatcccgcc agatatagta gtctcgctca gggctattag gattttcaca ggcctctaca
     1741 aaccaggcat gctcatccga ggtatgattg accaccaagt ccatgatgat acggatatca
     1801 cgcttcttag cttctgcaat cagctgatcc atgtcctcca tggttccaaa aatagccgca
     1861 atcgcttgat aatcagcaat atcgtagcca ttatcatcca tagggctgtc ataaacggga
     1921 gaaagccaaa ttgctgtgat tcctagcttg gctagatagt ccaacttact ggtaattcct
     1981 ggcaaatcgc caattccatc tccattacta tccataaaac tctttggata gacttgatag
     2041 actacggcat tatgccacca tttttcttgc atctgtttac ctcattattt tcataatcta
     2101 tagtctatga tagcactttt taaaattttg tgcaagcgtt tgcttaatct ttttaatttc
     2161 ttttttagct ccgtagtttc ctaacttcca attttttatt ataatagagt ccagaggtaa
     2221 aaaaatgaaa aaacaagctt ttagttctga acagtatttg aatttacaac gcgaccatat
     2281 tttggagcgc attaaccaat ttgacggcaa gctctactta gagtttggcg gtaaaatgtt
     2341 agaagatttc cacgctgctc gtgtccttcc tggttatgaa cctgacaaca aaatcaagct
     2401 cttgcaagaa ttgaaagagc aggttgaggt tgtgattgcc attaatgcta gcaacattga
     2461 acattccaaa gcacgtggcg acttgggcat ttcttatgac caagaagttc ttcgtttgat
     2521 tgacaaattc aatgaattag ggatttttgt tggttccgtt gtcattacac aatacgctgg
     2581 ccaacccgct gcagatgcct tcctcaacca acttgagaaa aacggaattg attcttatct
     2641 tcattatcca atcaaaggat atccgacgga tatggatcac atcatttccc cagaaggcat
     2701 gggcaaaaac gactacatca aaaccagtcg caacttgatt gtcgtaaccg ctcctggacc
     2761 cggttctgga aaattggcaa cgtgtatgtc caatatgtac cacgaccaaa tcaatggcat
     2821 caagtctggc tacgctaaat ttgaaacctt ccctgtttgg aatcttcccc ttcatcatcc
     2881 agttaacttg gcttatgaag ctgccacagc tgaccttgat gatgtcaaca tgattgaccc
     2941 cttccatctt caaacctatg gagaaaccac tgtcaactac aaccgtgata tcgaaatctt
     3001 cccagtgctc aagcgcatgt tggaacgtat tctcggaaaa tcaccatacg cttcaccgac
     3061 agatatgggt gtcaacatgg ttggtttcgc tattacagat gacgaggctg ctgtcgaagc
     3121 ttctaaacaa gaaatcatcc gccgttacta tcaaacagtt cttgacttca aagctgaaaa
     3181 agttggcgaa gctgccgtca agaaaattga gttgctcatg aacgacctcg gtatctcacc
     3241 tgcagaccgt aaggttgctg tcgttgcgcg ccaaaaagca gaagaaactg gcggaccagc
     3301 cctagctctt gaattaccaa atggtgacat tgtcactggt aaaaactcag aactctttgg
     3361 ccctacagcc gctgccttga tcaacgccat caaaaaatca gctgacatcg ctaaagaagt
     3421 aaaactaatc gagcctgaag ttgttaagcc aatccaagga cttaaaatcg atcatctcgg
     3481 tagccgcaat ccacgccttc attcaaatga aatcctgatt gcacttgcta tcacagctac
     3541 agaaaatcct gatgctgccc gcgctatgga agaactcggc aacctcaaag gaagcgaagc
     3601 ccactcaacc atcatcttga ctgatgaaga caagaatgtc cttcgtaaac tgggtatcaa
     3661 cgtaaccttt gacccatact accaatacga ccgcttatat cgtaagtaaa gatttcaacc
     3721 tctccagcct cggagaggtt ttctctctgt ctcaggagca agctttatga tataatgaga
     3781 gaagaaaact gaaaggattt accatgtcaa aagaagttat tgtcgaaagt tttgaacttg
     3841 accacaccat tgtcaaagca ccctatgttc gcttgattgg ggaagaaaca ggaccaaaag
     3901 gagacatcat ctccaattat gatattcgct tggtgcaacc aaacgaagac tctatcccta
     3961 ctgccggcct tcacactatc gagcacctct tagccaaact catccgtacc cgcattgacg
     4021 gcatgattga ctgttcacca tttggttgcc gcacaggctt ccacatgatt atgtggggac
     4081 gtcacaccag tgctaaaatc gcagctgtta tcaaggattc gctcaaggaa atcgctgaaa
     4141 ctactacttg ggaagatgtc ccagggacaa ccatcgaatc ttgcggaaac tacaaggacc
     4201 acagcctctt ctctgctaaa gaatgggcaa aactcatctt ggaacaaggg atttcagatg
     4261 atgcctttga acgtcatgtg atttaaaatc gagtagggga taatctctag cccctctcac
     4321 accaccgtac gtgccgttcg gcatacggcg gttcaactaa cttttaacgc atgtcgttca
     4381 aggtaataat ccaaacacga aaccaatcca cgtttttcaa ggactggttt tgatatagca
     4441 cgtttaagta ccgacttctg agctactata gtggattgaa actagaatag ttcacctctg
     4501 cttctaaaac attgttagaa atcgatttga ctatcctgat cgatttgttc tattcttatt
     4561 tcattttact ataattgata gtggtcgccc cagccagata ccttatctgc tatccattta
     4621 ggaaccccta acttaagcaa tccccataat cgtctcgatt tcttcttcca ttgcttccag
     4681 ataatcactc gtaggcgagt atgcaaacgc tcatctatgc tgg
//
LOCUS       DAVBCV010000091         4552 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBCV010000091 DAVBCV010000000
VERSION     DAVBCV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.091"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(112..549)
                     /locus_tag="VQ516_002012"
     CDS             complement(112..549)
                     /locus_tag="VQ516_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2512396.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(634..3267)
                     /gene="polA"
                     /locus_tag="VQ516_002013"
     CDS             complement(634..3267)
                     /gene="polA"
                     /locus_tag="VQ516_002013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2512397.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3520..3873
                     /locus_tag="VQ516_002014"
     CDS             3520..3873
                     /locus_tag="VQ516_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2512398.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            4088..4204
                     /locus_tag="VQ516_002015"
     CDS             4088..4204
                     /locus_tag="VQ516_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2512399.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            4197..4430
                     /locus_tag="VQ516_002016"
     CDS             4197..4430
                     /locus_tag="VQ516_002016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512400.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT         1385 a         1096 c          837 g         1234 t
ORIGIN      
        1 gtatctacaa cctcaaaaca gtgttttgag cagcccgcga ctagtttcct agtttgctct
       61 ttgattttca ttgagtataa cacaaaaggt agcccatcag ctaccttttt cttatgcttc
      121 ctcaatcaag cgagtatgtt ctctcttgat acagcgattc atcacgatat catcacatcc
      181 accatcacgc aaaatctctt tcgcttctaa actttcaagt cctagctgtg cccaaaaaat
      241 cttggcatca gctttgagaa aatcacgcgc cacatcgggc agaaattcac tgcgacgata
      301 aacattgaca atatctacag gaaaaggaat ttcagcgagg ctagcataag ccttttcacc
      361 caagatttcg ccacctgccg ccttgggatt gactgggatg attttatagc cccgagcctg
      421 catttccttt gtcactcgat tgctggttgt ttcttcacgg tcagacaaac ccaccacagc
      481 aagggtttta ctcgttgcga gatactgacg aatcacgcca tcacttggat tgataaattc
      541 ttgactcata gaaaacctcc tttttcatca gtatagcaca ttttgaaaag gtttgcagaa
      601 ttctactaca aaaaaggagg actagccccc tttttattta gcctcgtacc aggttgcccc
      661 ttcattctca tctgcgataa gaggaacact gagttgaatg gcttcttcca tggtttgttt
      721 caccaatttt ttcatctcta ccaattcaga tttaggcact tcaaggacga tttcatcgtg
      781 cacttgtaac agcatcttag tctgataacc acctgcaacc aaggctttat ccagctgaat
      841 catggcaatc ttgagaatat ctgctgccga accctggata ggtgagttga tagcagttcg
      901 ctccgcaaaa ccacgaatat tgaagttgcg cgaattgata tctggcaact cacggcgacg
      961 cttaaagagg gtctctacat agcccttatc acgcgcctcc cgcaccactt catccatgta
     1021 gtttttaata cctggaaaac gttcaaagta ggtatcaatg taggctttgg cttccttacg
     1081 actaattccc aaattattag acaagccaaa gtctgaaatc ccataaacca ctccaaagtt
     1141 aactgccttg gcattgcgac gatcatttgc agtcacatta tcaggacgct caatgccaaa
     1201 gacccgcatg gctgtcgaag tatggatatc tgccccctct tggaaggcct taatcaagtg
     1261 ctcatcctta gaaatatgcg ccaaaacgcg caattcaatc tgtgaatagt cagagctgag
     1321 tagcacacta tcctcccact ctggcacaaa agccttccga atcaagcgcc cttgttccaa
     1381 tcgggcagga atattttgca agtttggatc cacactagac aaacgcccgg tctgggtcaa
     1441 atcctgcata tagcgagtat gaatctttcc atcagccaaa atccagtcct gcaagccaat
     1501 tacataagta gattgaatct tagcaatttg acggtaatcc aggattttct taacaatcgg
     1561 agcaatagga gcgagacgct ccaacacatc cactgctgtc gaataacctg tcttggtttt
     1621 cttagtgtat tctagaggaa gtcccaattt ctcaaagaga agcacgccca actgcttagg
     1681 cgagttgaca ttaaactcct caccagccag ctcgtaaatc tcttgagtca gtttttcaat
     1741 gacaagctca ttttcagcct gcatctcaag caaggtctct ttcttgacca caatcccagc
     1801 aatttccatc ttggcaagaa caaaagccag aggttgctcc atatcataaa gaagctctaa
     1861 ttgcccattt tcgctgagtt tttcaagtaa aataggctct gtttctacca aaacagcaag
     1921 tttacaagct aagtgttcca agaatttctc acgttcagga atggcctttt taacaccctt
     1981 accgtagaaa gtttcatcat caaccaagta agtctgacca taaagactag cgatggtcgc
     2041 aatttcattg tcctccacag tcgaaaggag gtatttagcc aaacggatgt caaaagcagg
     2101 cgcctgcaaa tccacaccaa aacgttgcaa aagaacttta accttcttaa agtcataaac
     2161 tctcagagat gttttttcta agaaatcctt gaaaatcggg tcttgcaaca gctcaagctt
     2221 gtctgtggca tagagcttat ccccacaaga ccaggcaaat ccaaccaaat tatccgtatg
     2281 ataattctca ccaaaaagct caaagtggaa gatagactct tcactcagca tatcttgact
     2341 gatttggtca acaatagtaa aatccaaacc ctcagccaca tcagctgacg acacatttaa
     2401 agcctgcttg agctgtttga atcccatctc atcgtaaaat ttcccaagat tttcaacatc
     2461 tggaccacta tagaccaagt cctctaaacc aatcgcaatc ggtgccttgg tatcaatggt
     2521 cgctagtgtt ttagacaaaa aggcctgttc cttgtcattg atgagatttt ccttcatctt
     2581 agaagtcttc attccatcaa tattttcata aatcccctca agcgaaccat gctccagcaa
     2641 gagcttaata cccgtctttt caccgacttt ggtcacccca gggatattat ccgacttatc
     2701 acccatgagc gccttgagat cgataaactg agctggtgtg aggcccattt cttccatgag
     2761 gtaatctggc gtaaaggcct caaactcagc cacacctttc ttggaaattt caaccaccgt
     2821 atgctcatcc gtcagctgaa tcaaatcctt gtccccactg acaatggtaa tatcaaaacc
     2881 atcctgctct gctagcttat ccaacgtccc aatgatgtca tccgcctcat actgagccag
     2941 ctcatagtga cgaatcccca tatgatccag caactcacga atgaaaggaa attgctcacg
     3001 aaactcatca ggagtcttgg cccgaccacc cttatagtcc gcatacatct ctgtccggaa
     3061 ggtcgtcttt cccgcatcaa aagccaccaa aatatgactc ggctcaaccc gctccaacaa
     3121 atgactcaac atcaactgaa aaccataaat cgcattggta tgcaaaccag ccgcattctt
     3181 aaaacggtcc aactgctgat acagcgcaaa aaacgcccga aaagctacag aagacccatc
     3241 aatcaataat aattttttct tatccataca cccattataa aggaaagaat caaaaaatac
     3301 cattgggaag agctagagca agtatttttc aaactttttc cgaataaata gatagagcca
     3361 gagaatttag taaacctaga tttaaaaatg tgctataata tagtatattg aatctataat
     3421 agtacacctt gactgctaaa atatttctat aaattaattt gactttcctg atagagttgt
     3481 tcacatctta tttcaattca ctatagaagg aggaaaagga tgattctcag acatctggga
     3541 attagcccga ccaatgactt ggttgctaag aaaattttca gcaacccaga aatcacttgt
     3601 caatttatcc gcgatatgct ggacttgcca gccaaaaatg tgactatttt ggagggaagt
     3661 aacattcatg tcttgccttc catgccctac tcagcgcagg atttttatac cagtatagac
     3721 gtcttggcgg agttggataa cggtactcaa gtaattattg agattcaagt ccatcatcag
     3781 aattttttca tcaatcactt gtgggcttac ctgtgcagtc aggttaatca aaatcttgaa
     3841 aaaattcgtc agcgagaagg tgatactcac tagatctaca aacacatcgc tcctgtttac
     3901 gtcattgcta tcgtggatag taattatttc tcagatgacc tggcttttca tagctttagt
     3961 atgcgcgaag acacaacagg tgaggtattg gcgattacca acaatggaca ggaaaaccat
     4021 ctggttaaga tggcattctt ggaattaaaa aatacagaga aaccagcaaa gacaaggttc
     4081 gcaagccatg gttggagttt ttcggcaaca agccctttac ccagcaaccg caacgagcca
     4141 ttacccaagc aaatcaactg ctggactaca agagctggtc cgaggaggac aggaaaatgt
     4201 ttagtcaact acatatgcga gaagaacaag tcttgttagc acaggactat gccttggaaa
     4261 ctgctagggc tgaaggcctt gaacaaggac tagagcgtgg gaaagttgaa ggaagggcag
     4321 aaaggaaact ttttgccttc ctagacatag tacgccaagg tcttctgact tctgaggttg
     4381 ccagccagca attaggtatg tcagtatctg aatttgaggc actgttgtaa aatggctcca
     4441 taatatccat agtgggtaaa tcccctatgg atattatgga gcctattttt gtgtagaaaa
     4501 aaagtcccat atgacctata atgaaaagcg acaaaacaac tcattaaaaa ga
//
LOCUS       DAVBCV010000092         4477 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBCV010000092 DAVBCV010000000
VERSION     DAVBCV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.092"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..1261
                     /locus_tag="VQ516_002017"
     rRNA            <1..1261
                     /locus_tag="VQ516_002017"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1313..1385
                     /locus_tag="VQ516_002018"
     tRNA            1313..1385
                     /locus_tag="VQ516_002018"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1346..1348,aa:Ala,seq:tgc)
     gene            1510..4411
                     /locus_tag="VQ516_002019"
     rRNA            1510..4411
                     /locus_tag="VQ516_002019"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1239 a          918 c         1333 g          987 t
ORIGIN      
        1 atacatagcc gacctgagag ggtgatcggc cacactggga ctgagacacg gcccagactc
       61 ctacgggagg cagcagtagg gaatcttcgg caatggacgg aagtctgacc gagcaacgcc
      121 gcgtgagtga agaaggtttt cggatcgtaa agctctgttg taagagaaga acgagtgtga
      181 gagtggaaag ttcacactgt gacggtatct taccagaaag ggacggctaa ctacgtgcca
      241 gcagccgcgg taatacgtag gtcccgagcg ttgtccggat ttattgggcg taaagcgagc
      301 gcaggcggtt agataagtct gaagttaaag gctgtggctt aaccatagta ggctttggaa
      361 actgtttaac ttgagtgcaa gaggggagag tggaattcca tgtgtagcgg tgaaatgcgt
      421 agatatatgg aggaacaccg gtggcgaaag cggctctctg gcttgtaact gacgctgagg
      481 ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgct gtaaacgatg
      541 agtgctaggt gttagaccct ttccggggtt tagtgccgta gctaacgcat taagcactcc
      601 gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc
      661 ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatccc
      721 tctgaccgct ctagagatag agttttcctt cgggacagag gtgacaggtg gtgcatggtt
      781 gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca acccctattg
      841 ttagttgcca tcatttagtt gggcactcta gcgagactgc cggtaataaa ccggaggaag
      901 gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg tgctacaatg
      961 gctggtacaa cgagtcgcaa gccggtgacg gcaagctaat ctcttaaagc cagtctcagt
     1021 tcggattgta ggctgcaact cgcctacatg aagtcggaat cgctagtaat cgcggatcag
     1081 cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac cacgagagtt
     1141 tgtaacaccc gaagtcggtg aggtaaccgt aaggagccag ccgcctaagg tgggatagat
     1201 gattggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga tcacctcctt
     1261 tctaaggata aggaactgcg cattggtctt gtttagtctt gagaggtctt gtggggcctt
     1321 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga tcccgctagg
     1381 ctccattggt gagagatcac caagtaatgc acattgaaaa ttgaatatct atatcaaata
     1441 gtaacaagaa aataaaccga aaacgctgta gtattaataa gagtttatga ctgaaaggtc
     1501 agaaaataag gttaagttaa taagggcgca cggtggatgc cttggcacta ggagccgacg
     1561 aaggacgtga caaacgacga tatgccttgg gtagctgtaa gtaagcgatg atccagggat
     1621 ttccgaatgg gggaacccaa caggtagtac ctgttaccca catctgttaa ggatgtgagg
     1681 aggaagacgc agtgaactga aacatctaag tagctgcagg aagagaaagc aaaagcgatt
     1741 gccttagtag cggcgagcga aacggcagga gggcaaaccg aagagtttac tcttcggggt
     1801 tgtaggactg caatgtggac tcaaagatta tagaagaatg atttgggaag atcagccaaa
     1861 gagagtaata gcctcgtatt taaaatagtc tttgtaccta gcagtatcct gagtacggcg
     1921 ggacacgtga aatcccgtcg gaatctggga ggaccatctc ccaaccctaa atactcccta
     1981 gtgaccgata gtgaaccagt accgtgaggg aaaggtgaaa agcaccccgg gaggggagtg
     2041 aaatagaacc tgaaaccgtg tgcctacaac aagttcgagc ccgttaatgg gtgagagcgt
     2101 gccttttgta gaatgaaccg gcgagttacg ttatgatgcg aggttaagtt gaagagacgg
     2161 agccgtaggg aaaccgagtc tgaatagggc gccttagtat catgacgtag acccgaaacc
     2221 atgtgaccta cccatgagca ggttgaaggt gcggtaagac gcactggagg accgaaccag
     2281 ggcacgttga aaagtgcttg gatgacttgt gggtagcgga gaaattccaa acgaacttgg
     2341 agatagctgg ttctctccga aatagcttta gggctagcgt cgacattaga gattcttgga
     2401 ggtagagcac tgtttgggtg aggggtccat cccggattac caatctcaga taaactccga
     2461 atgccaatga attatggtcg gcagtcagac tgcgagtgct aagatccgta gtcgaaaggg
     2521 aaacagccca gaccaccagc taaggtccca aaataattgt taagtggaaa aggatgtggg
     2581 gttgcacaga caactaggat gttagcttag aagcagctat tcattcaaag agtgcgtaat
     2641 agctcactag tcgagtgacc ctgcgccgaa aatgtaccgg ggctaaaaca atttaccgaa
     2701 gctgtggata cctttatagg tatggtagga gagcgttcta tgtgtgatga aggtataccg
     2761 tgaggagtgc tggaacgcat agaagtgaga atgccggtat gagtagcgaa agacaggtga
     2821 gaatcctgtc caccgtaaga ctaaggtttc caggggaagg ctcgtccgcc ctgggttagt
     2881 cgggacctaa ggagagaccg aaaggtgtat ccgatggaca acaggttgat attcctgtac
     2941 tagagtatgt agtgatggag ggacgcagta ggctaactaa agcagacgat tggaagagtc
     3001 tgtctaagca gtgaggtgtg atatgagtca aatgcttata tctgtaacat tgagctgtga
     3061 tggggagcga agtttagtag cgaagttagt gacgtcacac tgccaagaaa agcttctagc
     3121 gtttaaacat actctacccg taccgcaaac cgacacaggt agtcgaggcg agtagcctca
     3181 ggtgagcgag agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa
     3241 ggggtgctga ctttaagtca gccgcagtga ataggcccaa gcaactgttt atcaaaaaca
     3301 cagctctctg ctaaatcgta agatgatgta tagggggtga cgcctgcccg gtgctggaag
     3361 gttaagagga gtgcttagcg taagcgaagg tatgaattga agccccagta aacggcggcc
     3421 gtaactataa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acccgcacga
     3481 aaggcgtaat gatttgggca ctgtctcaac gagagactcg gtgaaatttt agtacctgtg
     3541 aagatgcagg ttacccgcga caggacggaa agaccccatg gagctttact gcagtttgat
     3601 attgagtgtc tgtaccacat gtacaggata ggtaggagtc taagagatcg ggacgccagt
     3661 ttcgaaggag acgctgttgg gatactaccc ttgtgttatg gccactctaa cccagatagg
     3721 tgatccctat cggagacagt gtctgacggg cagtttgact ggggcggtcg cctcctaaaa
     3781 ggtaacggag gcgcccaaag gttccctcag aatggttgga aatcattcgc agagtgtaaa
     3841 ggtataaggg agcttgactg cgagagctac aactcgagca gggacgaaag tcgggcttag
     3901 tgatccggtg gttccgtatg gaagggccat cgctcaacgg ataaaagcta ccctggggat
     3961 aacaggctta tctcccccaa gagttcacat cgacggggag gtttggcacc tcgatgtcgg
     4021 ctcgtcgcat cctggggctg tagtcggtcc caagggttgg gctgttcgcc cattaaagcg
     4081 gcacgcgagc tgggttcaga acgtcgtgag acagttcggt ccctatccgt cgcgggcgta
     4141 ggaaatttga gaggatctgc tcctagtacg agaggaccag agtggactta ccgctggtgt
     4201 accagttgtc ttgccaaagg catcgctggg tagctatgta gggaagggat aaacgctgaa
     4261 agcatctaag tgtgaaaccc acctcaagat gagatttccc atgattatat atcagtaaga
     4321 gccctgagag atgatcaggt agataggtta gaggtggaag tgtggcgaca catgtagcgg
     4381 actaatacta atagctcgag gacttatcca aagtaactga gaatatgaaa gcgaacggtt
     4441 ttcttgaatt gaatagatat tcgattttga gtaggta
//
LOCUS       DAVBCV010000093         4438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBCV010000093 DAVBCV010000000
VERSION     DAVBCV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.093"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..119
                     /locus_tag="VQ516_002020"
     CDS             <1..119
                     /locus_tag="VQ516_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2512401.1"
                     /translation="QQGFTDRLVGYSSSIALVFTTLIGMISLVQRRVLKEDD"
     gene            121..966
                     /locus_tag="VQ516_002021"
     CDS             121..966
                     /locus_tag="VQ516_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2512402.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            976..2295
                     /locus_tag="VQ516_002022"
                     /pseudo
     CDS             976..2295
                     /locus_tag="VQ516_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
     gene            complement(2503..2575)
                     /locus_tag="VQ516_002023"
     tRNA            complement(2503..2575)
                     /locus_tag="VQ516_002023"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2540..2542),aa:Lys,seq:ctt)
     gene            complement(2839..4110)
                     /locus_tag="VQ516_002024"
     CDS             complement(2839..4110)
                     /locus_tag="VQ516_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2512403.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
BASE COUNT         1295 a          809 c          975 g         1359 t
ORIGIN      
        1 atcaacaagg ttttactgat cgtcttgtag gatattctag ctcaatcgct ttagtattta
       61 caacgttaat tggtatgatt agcttagtac aacgtcgcgt tctgaaggag gatgactaag
      121 atgaaacgat ttaaaccaac aacaatctta gaatatattc tgttaacttt attggcagga
      181 ctcttccttt ttcctatggt ttggatggta gtttcagcta tgaagccaga agcggatgtt
      241 tataaaaatt tgactagttg gcaagccttc ttaccaagtt taaatcccgc tgattggttt
      301 aaaccttatc aggaagtatt tgagcgcttt agtattttta catatctagg gaacagcatt
      361 ttttatgctg gaacgtttgc tattggttct attattgtca atgcactggc cggtttcgct
      421 tttgcaaaag tcaatttttc aggtaagaaa attttatttg gtttcttact tgctttactt
      481 attattccag tggaaactgt gttaattcca caatttacca ttatcaattc cttaggtttg
      541 gtcaataatc gtcttgcagt tattattcca ggattggcga gtgtatttaa tatttactta
      601 tttaggaatt tctttattgc aattccagaa gagattcttg agtctgcaaa gatggacgga
      661 gcaagtatcg ttagaatttt ctttagaatt atgcttccaa tgtctaaacc tgcagttgct
      721 acagttggtg ttttatcatt tatttcaagc tggaatgatt atatctggcc attgatggtt
      781 ttgacagatt cttctaaatt ttcaatgcag gttgcaatta caactattaa tacaactcaa
      841 ccagtttata tcaatcaggt aatggctgta ttaactattt caacgattcc gttgattttg
      901 gtctatgtag tcgctcagaa atatattgtt caaggtctgg gtggttccgg aactggaata
      961 aaataggata tacttatgat aaataaaaca tttacagtag aaaaagccaa tcgttttata
     1021 gcagaaaata aacatctcgt taatactcaa tataagcctg aagaacattt ttcagctgag
     1081 attggttgga tcaatgatcc aaatggattt gtctattttc gtggagaata ccatctcttt
     1141 tatcaattct atccatatga tagtgtttgg gggcctatgc actggggaca tgctaaaagt
     1201 aaggacttgg tgacttggga gcacttgcca gtggcacttg ctcctgacca agattatgac
     1261 cgaaatggtt gtttctcagg ctctgccatt gtcaaggatg atcgcctctg gctcatgtac
     1321 actggacata tcgaagaaga aaccggtgtc cgccaagtgc aaaatatggc attttcagat
     1381 gacgggattc actttgaaaa gatttcccaa aatccagttg caactggatc agacttgcca
     1441 gatgagttga ttgctgctga tttccgtgat ccaaaactct ttgaaaaaga tggacgctat
     1501 tactccgtag tagctgccaa acacaaggat aatgtgggct gtatcgttct actagggtcc
     1561 gataacctag tagaatggca gttcgaatcc atctttttaa aaggaggaga acaccaaggc
     1621 tttatgtggg aatgcccaga ttacttcgag ttagatggga aagattgcct tattatgtca
     1681 cccatgcgtt atcagcgtga gggagactca tatcataaca tcaactcatc gcttttgttc
     1741 acgggtaagg tagattggag agaaaaacgt tttatcccag aatcagttca agaaattgat
     1801 catggccaag acttctatgc gcctcaaaca ttgttggacg atcaaaatcg tcgtatcctg
     1861 attgcttgga tgcagacatg ggggcgtacc cttccaaccc atgaccaaga acacaaatga
     1921 gcatgtgcca tgactttacc tagaattcta agattggaag atggcaaact aagacaattc
     1981 cctgttaaaa aaggccaata tcaaatccaa atagataaag attgtcatta ccacttagga
     2041 aatgatacag attatcttga atttggttat gacagtaatg cgcagcaagt ttacattgat
     2101 cgtagccatc ttattcaaaa aattctaggt gaagaagaac aggacactag tcgacggtat
     2161 gtagatattg aagctaaaga attggaagtt gttctagata aaaattccat cgagattttt
     2221 gtcaatcaag gtgaagcaag cttgactgca acttattact taacggtgcc agctgagcta
     2281 tcacgaattg attaaaaatt aagttatttc tcctaaagaa aaagttctct ttctaaaata
     2341 gtggaaagag gactttttgt gttttgggta tataagctta gtttatggta tttgtaaaat
     2401 tggtgttgga ttatgattta agctagtttt ctaaagaatt tgaaaaaaat tttatttaag
     2461 caaaaaaacc ttggttccaa ggcttttcct gttgtattta gatgccccct acagggatcg
     2521 aacctgtgac ccacggatta agagtccgct gctctgccag ctgagctaag gaggcaaaga
     2581 aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa ttaagcaggt
     2641 gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc tgttagaaga
     2701 cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg aagtcgtaca
     2761 ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag aggaaagtgc
     2821 aattttttga aaaggatttc agatttttcg caaacgatcg atagcttcct ttctttgagc
     2881 caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat tacgaatttt
     2941 ttctggcaga tagtcttgct tgatccaatt tccaggatag ttgtgtggat agagatagtc
     3001 ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgcaggt gtcgcggaat
     3061 aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta cataggctga
     3121 gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa tgcgggcttc
     3181 ggggaaacca atcctttgtg cagcatccag agcagtcacg gtatgaatct gggcctcagg
     3241 attggctaag ccaatatctt cataggcgat aacagtcaag cgacgagcga gactaggcag
     3301 atccccagcc tcaatcaagc gggcagcata gtggagactg gcatccacat ctgagccacg
     3361 gatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct tgtccatagt
     3421 gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc gaatgccctc
     3481 gtcattctca ggggtagaga gaacagccaa gtccagtgag ttaaaggcag agcgaaggtc
     3541 tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta ttggaaaatc
     3601 aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt cttggttaga
     3661 cagaggttcc aactcgaaaa tttgaacacg gctacgaatg gcaggagtga cagagaagaa
     3721 aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga gaggcaagag
     3781 gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga cgagaccacc
     3841 agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa ctgtcgcatt
     3901 gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac tggttttgcc
     3961 gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca ccatgcggcg
     4021 gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga tggttttagg
     4081 gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctaacgtgg atttgagtag
     4141 aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt gatatttcaa
     4201 tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat ttaatgagga
     4261 gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata gttgagccta
     4321 aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta tgaactataa
     4381 tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt ttgatttt
//
LOCUS       DAVBCV010000094         4137 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBCV010000094 DAVBCV010000000
VERSION     DAVBCV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.094"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            720..1712
                     /locus_tag="VQ516_002025"
     CDS             720..1712
                     /locus_tag="VQ516_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2512404.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VQ516_002026"
     CDS             1714..2532
                     /locus_tag="VQ516_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2512405.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VQ516_002027"
     CDS             2534..3319
                     /locus_tag="VQ516_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2512406.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3358..3636
                     /locus_tag="VQ516_002028"
     CDS             3358..3636
                     /locus_tag="VQ516_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2512407.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3621..3818
                     /locus_tag="VQ516_002029"
     CDS             3621..3818
                     /locus_tag="VQ516_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2512408.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1152 a          705 c          897 g         1383 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cgatgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
      541 gagaaatcct aatttcacaa tccataggca aactcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagcaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccagact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgacg gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtcattt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tctccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttgatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggggagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccga aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagaa acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaagg cagttgtagc tgaggttttg
     3421 aaggatttgc cagaatggtt tggaatccca gaaagcacac aagcctatat agaaggaacc
     3481 acgacactgc aagtttggac cgcctatcag gagagtgatt tgactagatt tgtaagctta
     3541 tcctattcga gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag
     3601 gtagaaaaat tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg
     3661 gttatctgca ggtcaaaaca gtggcagaag gttctaataa agattatgat cgaacaaatg
     3721 acttttatcg aggtcttggc tttaaaaagt tagagatttt tcctcaacta tggaatccgc
     3781 aaaatccttg tcagattttg attaaaaagc ttgaataata ttacttgaca tctattctca
     3841 gagtgccata ctgtaagtgt aatcgccgat ttagctcagt tggtagagca aggcactcgt
     3901 aaagcctagg ttataggtag ataaacgact gaggagttga aaaaatagat aggtagaaga
     3961 taaccgttaa gccttattct tagcggttat ttatattgtt taatagcgct gatatttcat
     4021 caattatgcc tgttttcgtg tttctggtag ttgttcaagt ttattgctac tatttttgat
     4081 ggtatgaatg tgcttataat gtatcccggt taacgaaagt tttggactta tactctt
//
LOCUS       DAVBCV010000095         4116 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBCV010000095 DAVBCV010000000
VERSION     DAVBCV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.095"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..776
                     /locus_tag="VQ516_002030"
     CDS             <1..776
                     /locus_tag="VQ516_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512409.1"
                     /translation="DYSIVEEKLDWQSKLSKIYPNVEFIKIEKLKLILLCKYRNQIID
                     LEDDKKRLLLLKLLYLTSKQFCYDEFKGAKFGCMSFFSHYQGYMSRLDSLDSDKFVSV
                     KQRIVSSISKLVEQYSDHIINLDRTLVDSLSDTLENQLFDDWDYILSEITTYSELLAK
                     SGLLYNSLDYKIDNVKKEVAKLSDFHQSLFKIEGFSDFFDSWQFQRYRSLVNQVYEIF
                     SMLQVSSVERTVLCGALSTIVFSKSNFNYQAFFEYLKDT"
     gene            805..1713
                     /locus_tag="VQ516_002031"
     CDS             805..1713
                     /locus_tag="VQ516_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512410.1"
                     /translation="MDYVDVILDEYGSVFLIKDHIKLKINNLELYRYFSSFGMSHLQF
                     RKEELPGKLKQFDFEKNKSDSYFSKKSSLIDFIKRNYFYDIKLYSDNPKNPIESWKNK
                     TEKIKIAKSTEILWSEVANFSSISKSPYYCLLYNLKNTENYILIISKTYFSAIQKMVS
                     YNLNNYFELEDVCCKSIVSKRELIENSININKSKMKLLNVCEGSKGYTRRVFKYNNIF
                     VGQLEFHSNIIFSEIALNELSIVKLLDSVESVLSEQLFGKLNWDNLFVVLSDDFNDYL
                     DINESQKYYSNGSLRGLELGIFMGGV"
     gene            1715..3613
                     /locus_tag="VQ516_002032"
     CDS             1715..3613
                     /locus_tag="VQ516_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET2512411.1"
                     /translation="MRIIEIVDVEDQLLQLLSAHTNIDIRAQELSTLDPDSKVDLIIY
                     LKSRRDIENINNIIIKSPNRNIIVIISAFDIIYALYFPKSTFRPCLNCLLLKLKDNNP
                     EHNLLLFLKYGTLPSKHFNLDNRLKSNLILFNEKLDPKVEEYSIEGVNTTNFTFHFSC
                     SCENSGYDMSERMADILLGEGKKLSPKNYRLKKSEHLFDFTRYLDRNFGFFHHSYDEK
                     ISEYAPLHAVEINVDAGYESGWGRTFMTEAATKSAFLESLERYCNSRNRRYKKLIRGC
                     FSDLSKKYNTLNPVDFCLSNKEYRDSSYKFHDFDSEKNYDWIFGKNITTGKICLLPSQ
                     LVFYKDFSKSEENERFVYETSNGCALGSSIEEAIFYGILEIIERDNFLLTWYLKEEVK
                     QLSWESLPSRLRIINERLKEDNCELKLFNITKEVGIPVIWALYINHNENASIRSFSAA
                     GCHINPLSAIESAIFEVITSVPTFERLIEVRNKESFIEEIQNNFSLIRDIDDHILYHA
                     HADYKNKMPFLFKNNYSEYQNEFDENRIFNTYSHATLTSDLQALIKIIEKKFGDIYVV
                     NISDKVSEKFGLYCVKVLIPGLLNMSFGHSNRRVNIERIKNYVDYRGGNVNSITLNSI
                     NHLPHPFP"
     gene            3637..>4116
                     /locus_tag="VQ516_002033"
     CDS             3637..>4116
                     /locus_tag="VQ516_002033"
                     /inference="COORDINATES: protein motif:HMM:NF025394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET2512412.1"
                     /translation="MKRDWKSKHLFYHDVQQFDKLVIYLSKFMDKLVNQNQIEKYFYI
                     RYWEGGPHIRLRYLVRDTQNFETIDSMINKYAYKFWENNPPMKLLNKTDYKKIYNESI
                     DNNGKMDRKLRKNATIENIAYIPEIERYGGEEALIVSEDLFCICSQVSTALLNVVDNY
                     "
BASE COUNT         1539 a          443 c          712 g         1422 t
ORIGIN      
        1 cagattactc aatagtggaa gaaaaattag attggcaaag taaattatct aaaatttatc
       61 cgaatgttga atttataaaa attgaaaaat taaaattgat tttgttgtgt aaatatagaa
      121 atcaaattat tgacttagag gatgataaaa aaagattgtt actattgaaa ttattatatt
      181 tgacttcaaa gcaattttgc tatgatgaat ttaaaggagc gaaatttgga tgtatgtcat
      241 ttttttcaca ttatcaaggt tatatgagca gattagattc actcgactca gataaatttg
      301 tttcggttaa acagaggatt gtttcctcta tttctaaatt agttgagcaa tattcagatc
      361 atattataaa tctggataga actttagttg attctctaag tgataccctt gagaatcagc
      421 tatttgatga ttgggattat atattaagcg aaataactac atatagcgaa ttattagcaa
      481 aatcaggatt gttatataat tcgctagact ataagattga taatgtaaaa aaagaagtgg
      541 ccaagctaag tgactttcac caatctttat ttaaaattga aggattttca gatttttttg
      601 atagttggca atttcaaaga tataggtctc tcgtgaacca agtttatgaa attttttcaa
      661 tgttacaagt aagtagtgta gaaagaactg tgttgtgcgg agctctaagt actattgtct
      721 ttagtaagtc aaattttaac tatcaagctt tttttgagta tctaaaagat acgtagaatt
      781 cgattttaaa ggaggctgaa atatatggat tatgtagatg taatattgga tgagtatggg
      841 agtgtttttc tgataaagga tcacataaaa ctaaaaatta ataatctaga actatatcgg
      901 tattttagta gctttggaat gagtcatctt caatttagaa aagaagagct tccaggaaaa
      961 ttaaagcagt ttgattttga aaaaaataaa agtgacagtt atttttctaa gaaaagtagt
     1021 ctgattgatt ttattaagag aaattatttt tatgatatta agttatattc tgataatccg
     1081 aaaaatccga tagagtcttg gaaaaataag acagaaaaaa ttaagattgc taaaagtaca
     1141 gaaattcttt ggagtgaagt agcaaatttt tcgtcaattt ctaaatctcc ttactattgc
     1201 ttgttatata atttaaagaa tactgaaaat tacatattga taatcagtaa gacatatttt
     1261 tcagccatcc aaaaaatggt ttcgtataat ctaaataatt attttgaatt agaagatgtt
     1321 tgttgtaaaa gcatagtgag caagcgagaa ttgatagaaa attcaattaa tattaacaaa
     1381 agcaaaatga aactgctaaa tgtttgcgaa ggtagtaaag gttacacaag aagagtattt
     1441 aagtacaata atatttttgt gggacaattg gaatttcact ctaacataat cttttcagaa
     1501 attgctttaa atgaattaag tattgttaag cttttagatt ctgttgaaag tgttttatct
     1561 gagcaactat ttggaaaatt aaattgggat aatttatttg ttgttttatc agatgatttt
     1621 aatgattatc tggatataaa tgaatctcaa aaatactact caaatggttc tttgagagga
     1681 ctggagcttg gaatctttat gggaggggtt taaaatgaga ataattgaaa ttgttgatgt
     1741 tgaggaccaa cttttacaac ttttaagtgc acataccaat attgatatta gagcacaaga
     1801 gttatcaaca cttgacccag attcaaaagt tgatttgatc atatacttga aatctcgacg
     1861 tgatattgaa aacataaata atattattat taaatcacct aacaggaata tcattgttat
     1921 aataagtgct tttgatatta tatatgcatt gtattttcca aaatcaacat ttagaccttg
     1981 tttgaactgt ctattattaa aacttaaaga taacaaccct gagcataatc tattattatt
     2041 cctgaaatac ggaacattac ctagtaaaca ttttaatctt gataataggt taaaatcaaa
     2101 tttaatacta tttaatgaga agttagaccc taaggtagag gagtatagta ttgagggggt
     2161 aaatactaca aattttactt tccattttag ttgtagctgt gaaaatagtg gttacgatat
     2221 gtcggaaaga atggcagata ttctacttgg tgaaggaaaa aaattatctc ctaaaaatta
     2281 tcgtttaaaa aagagtgagc atctattcga ttttactaga tatctagata gaaattttgg
     2341 attttttcat cattcatatg atgaaaaaat ctccgaatat gcgcctctcc atgcagtaga
     2401 gattaatgtt gatgctggtt acgagtcagg ctggggaagg acttttatga cagaggctgc
     2461 aacaaaatct gcttttttag agtcattaga gagatactgc aactctagaa atagacgcta
     2521 taaaaaatta attagagggt gtttttctga tttatctaaa aagtacaata cattaaatcc
     2581 tgttgatttt tgtctttcta ataaagaata tagagatagt tcctataaat ttcatgattt
     2641 tgattctgag aaaaattatg attggatatt tggtaaaaat ataactaccg gaaaaatttg
     2701 cttattgccc tcgcaactag tattttataa agactttagt aaaagcgaag aaaatgaaag
     2761 gtttgtttat gaaacatcaa atggatgtgc tttggggagt agtattgagg aagctatttt
     2821 ttatgggatt ttagagatta ttgaaagaga taatttttta cttacatggt atttaaaaga
     2881 agaagtaaaa caattatcat gggaatcttt accatctcgc ctacgtatta ttaatgaaag
     2941 actaaaagaa gataactgtg agctcaaact ttttaatata actaaagaag tgggcatacc
     3001 agtaatttgg gcattgtata taaatcataa tgaaaatgct agtattcgaa gtttttcagc
     3061 tgcaggctgt catattaatc ctttaagtgc aattgaatca gcaatttttg aagtaataac
     3121 ttctgtgcca acttttgaaa gattaattga agttagaaat aaagagtcat ttattgagga
     3181 aattcagaat aatttttctt taatacgaga tattgacgat catatattgt atcatgctca
     3241 tgcggattat aaaaataaaa tgcccttttt atttaaaaat aattatagtg aatatcaaaa
     3301 tgaatttgat gagaatagaa tttttaatac ttattcacat gcaacgctta catcagattt
     3361 acaggcacta ataaaaatta ttgaaaaaaa atttggagat atatatgtag tgaatataag
     3421 tgataaagtt tccgaaaaat ttggacttta ttgtgttaaa gtgttgattc ctggacttct
     3481 taatatgtca tttggacatt caaatcggcg tgtaaatata gagaggataa aaaattatgt
     3541 agattataga gggggaaatg taaatagtat tacattgaat tccataaacc atcttcccca
     3601 tccatttcca taagattata tataaaggaa taataaatga aaagagattg gaaatcaaag
     3661 catttatttt atcatgatgt tcaacaattt gataaattgg taatctattt atcaaaattt
     3721 atggataaat tagttaatca gaatcagatt gaaaaatatt tctatattcg ttattgggaa
     3781 ggggggcctc atattcgatt aagatatttg gtgagggata cacaaaattt tgaaacaatt
     3841 gatagtatga taaataagta tgcttataaa ttttgggaaa ataatcctcc aatgaagtta
     3901 ttaaataaga ccgattataa gaaaatttat aatgagagta ttgataacaa cggaaagatg
     3961 gatagaaaat tgaggaaaaa tgcgactatt gaaaacattg cctatatacc ggaaatagag
     4021 agatatggcg gggaagaagc tctgatagta agcgaagatc tattttgtat ctgtagtcag
     4081 gtttcaactg cgttacttaa tgtggtagat aactat
//
LOCUS       DAVBCV010000096         4006 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBCV010000096 DAVBCV010000000
VERSION     DAVBCV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.096"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            213..2114
                     /locus_tag="VQ516_002034"
     CDS             213..2114
                     /locus_tag="VQ516_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2512413.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINCVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            2117..2980
                     /locus_tag="VQ516_002035"
     CDS             2117..2980
                     /locus_tag="VQ516_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2512414.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            3060..>4006
                     /locus_tag="VQ516_002036"
     CDS             3060..>4006
                     /locus_tag="VQ516_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2512415.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQVIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFILVTFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTI"
BASE COUNT         1199 a          681 c          883 g         1243 t
ORIGIN      
        1 gctatacgcg tacgtaaata atgaaaagaa ttaaataata ggcttgtgcg ataactattt
       61 gaaacaagat tagctggtta gaaaatccaa caagatagtg aaggaatcta atataaaata
      121 tacataattt agtttatttt atgttataaa agttgtacta aaagacttac gattatcttg
      181 aatgccttag ggaaacatgc tatactactc ttatgattat tttacaagct aataaaattg
      241 aacgttcttt tgcaggagag gttcttttcg ataatatcaa cctgcaggtt gatgaacgag
      301 atcggattgc tcttgttggg aaaaatggtg caggtaagtc tactcttttg aagattttag
      361 ttggagaaga ggagccaact agcggagaaa tcaataagaa aaaagatatt tctctgtctt
      421 acctagccca agatagccgt tttgagtctg aaaataccat ctacgatgaa atgcttcatg
      481 tctttaatga tttgcgtcgg acggagagac aactgcgtca gatggagctg gagatgggtg
      541 aaaagtctgg tgaggatttg gataaactga tgtcagatta tgaccgctta tctgagaatt
      601 ttcgccaagc aggtggcttt acctatgaag ctgatattcg agcgattttg aatggattca
      661 agtttgacga gtctatgtgg cagatgaaaa tagctgaact ttcaggtggt caaaatactc
      721 gtttggctct agctaaaatg ctccttgaaa agcccaatct cttggtcttg gacgagccaa
      781 ctaaccactt ggatattgaa accatctcct ggctagagaa ttacttggta aactatagcg
      841 gtgccctcat tatcgtcagc cacgaccgtt atttcttgga caaggttgcg acaattacgc
      901 tagatttgac caagcattcc ttggatcgct atgtggggaa ttactctcgt tttgtcgagt
      961 tgaaggagca aaagctagct actgaggcaa aaaactatga aaagcaacag aaggaaatcg
     1021 ctgctctgga agactttgtc aatcgcaatc tagttcgtgc ttcaacgacc aaacgtgctc
     1081 aatctcgccg taaacaacta gaaaaaatgg agcgtttgga caagccggaa gctggcaaga
     1141 aagcagccaa catgaccttc cagtctgaaa aaacgtcggg caatgttgtt ttgactgttg
     1201 aaaatgcggc tgttggctat gatggggaag tcctgtcaca accgattaac ctagaccttc
     1261 gtaagatgaa tgctgttgct atcgttggtc ccaatggtat cggaaaatca acctttatca
     1321 aatcaatcgt ggatcaaatt ccgtttatca agggagaaaa gcgctttggc gctaatgttg
     1381 aggttggtta ctatgaccaa acccaaagca agctgacacc aagtaatacg gtgctggatg
     1441 aactctggaa tgatttcaaa ttgacaccag aagttgaaat ccgcaaccgt cttggagcct
     1501 tccttttctc aggagatgat gttaaaaaat cagtcggcat gctatctggt ggcgaaaaag
     1561 ctcgtttgct tttagctaaa ttgtctatgg aaaacaataa ctttttgatt ctggatgagc
     1621 cgaccaacca cttggatatt gatagtaagg aagtgctaga aaatgccttg attgactttg
     1681 atggtacctt gctttttgtc agtcatgatc gttactttat caattgtgtg gcaactcatg
     1741 ttttggaatt gtctgagaat ggttcaactc tctaccttgg agattacgac tactatgttg
     1801 agaagaaagc aacagcagaa atgagtcaga ctgaggaagc ttcaactagc aatcaagcaa
     1861 aggaagcaag tccagtcaat gactatcagg cccagaaaga aagtcaaaaa gaagttcgca
     1921 aactcatgcg acaaatcgaa agtctagaag ctgaaattga agagctagaa agtcaaagcc
     1981 aagccatttc tgaacaaatg ttggaaacaa acgatgccga caaactcatg gaattacagg
     2041 ctgagctgga taaaatcagc catcgtcagg aagaagctat gcttgagtgg gaagaattat
     2101 cagatcaggt gtaaagatgg aacatcttgg aaaagtattt cgtgaatttc gaacaagtgg
     2161 aaattattct ttaaaggaag cagcaggcga atcctgctct acctctcagt tatctcgctt
     2221 tgagcttggg gagtctgacc tggcagtctc ccgtttcttt gagattttgg ataacattca
     2281 tgtaacaatc gaaaatttca tggataaggc aaggaatttt cataatcatg aacatgtgtc
     2341 tatgatggca cagattatcc cactttacta ttcaaacgat attgcaggtt ttcaaaagct
     2401 tcaaagagaa caacttgaaa agtctaagag ttcgacgact tccctttatt ttgagctgaa
     2461 ctggattttg ctacaaggtc tgatttgtca aagagatgcg agttatgata tgaagcagga
     2521 tgatttggat aaggtagcag attatctctt caaaacagaa gaatggacca tgtatgagtt
     2581 gattcttttc ggtaacctct atagtttcta cgatgtagac tatgtcactc ggattggtag
     2641 agaagttatg gagagggagg aattttacca agagattagt cgccataaga gattagtgtt
     2701 gattttggcc ctcaattgtt accagcattg tttagagcat tcttcttttt ataatgccaa
     2761 ctattttgag gcttatacag agaagattat tgacaaaggt attaagcttt atgagcgtaa
     2821 tgttttccat tatttaaaag gttttgcctt atatcaaaaa ggacagtgta aagaaggctg
     2881 taagcagatg caagaggcca tgcatatttt tgatgtgtta ggtcttccag agcaagtagc
     2941 ctattatcag gaacactacg aaaaatttgt caaaagttaa ttttcccaaa taagggaaaa
     3001 aataaaaagc tcctttcggt tttgatacaa tagtttcaaa atttgagagg agctttaata
     3061 tgaatcgcta tgcagtgcag ttgattagcc gtggggctat caataaaatg ggaaatatgc
     3121 tctatgatta tggaaatagt gtctggttgg cttctatggg gactatagga cagacagttt
     3181 taggaatgta tcagatttct gagctcgtca catctattct cgtcaatccc tttggcggag
     3241 ttatttcaga ccgtttttct cgtcgtaaga ttttaatgac ggcagatctt gtttgtggga
     3301 ttctttgtct ggctatttct ttcataagga atgatagctg gatgattggc gctttgattg
     3361 ttgctaacat tgtgcaggtt attgcttttg ccttttctcg cacagccaat aaagctatca
     3421 taactgaagt ggtggagaaa gatgagattg tgatctataa ttctcgctta gagctggttt
     3481 tgcaggttgt aggtgttagc tctcctgttc tttccttcct tgttttacag tttgcaagtc
     3541 tccatatgac gctactgcta gactcgctga cttttttcat tgcttttatt ctagtgactt
     3601 tccttccaaa agaggaagca aaagttcaag agaaaaaggc ttttactggg agagatattt
     3661 ttgtagatat caaggatggg ttacactata tctggcatca gcaagaaatt ttcttccttt
     3721 tgctggtagc ttccagcgtt aatttctttt ttgcagcttt tgaatttcta cttccctttt
     3781 cgaatcagct ttacgggtca gaaggagcct atgcaagtat tttaactatg ggggctattg
     3841 gttccatcat tggggctctt ctagctagta aaattaaagc taatatttat aatcttttga
     3901 ttttactggc tttgacaggt gtcggagttt ttatgatggg attaccactt ccaacttttc
     3961 tttccttttc tggaaattta gtttgtgaat tgtttatgac gatttt
//
LOCUS       DAVBCV010000097         3693 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBCV010000097 DAVBCV010000000
VERSION     DAVBCV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.097"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(119..970)
                     /gene="rfbD"
                     /locus_tag="VQ516_002037"
                     /pseudo
     CDS             complement(119..970)
                     /gene="rfbD"
                     /locus_tag="VQ516_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1036..2085)
                     /gene="rfbB"
                     /locus_tag="VQ516_002038"
     CDS             complement(1036..2085)
                     /gene="rfbB"
                     /locus_tag="VQ516_002038"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2512416.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2095..2691)
                     /locus_tag="VQ516_002039"
     CDS             complement(2095..2691)
                     /locus_tag="VQ516_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2512417.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(2692..3549)
                     /gene="rfbA"
                     /locus_tag="VQ516_002040"
     CDS             complement(2692..3549)
                     /gene="rfbA"
                     /locus_tag="VQ516_002040"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2512418.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1028 a          857 c          662 g         1146 t
ORIGIN      
        1 ctgagccaaa gctatgcacc aaagagacca gcaccaacaa caagataatt gtacatctta
       61 agctcctttg gagttcatta tctgcctctt ttattagact agaaaatcat tctactactt
      121 atctcacttc ttgtttgtaa aattcttgca atgcatcttg ccaagttgga ataacaaatc
      181 cagtagcttt ggctttggcc aggctcatcg ttgagtttag cggacgttga gctttggctg
      241 gaaattgact ggaatctact ggcttgactt cgacatctgt atctttcaaa atttcaactg
      301 caaaatcata ccatgttgtg tcttctgtcg catcatttga caaatgataa taaccaaatt
      361 ccttacgatt ttcagctagg tagatcatga attcagccaa ggtacgagtc caagtcggac
      421 gaccgtactg gtcatttaca actgttaaag tcttatgagt tttcgcaaga ttttgcatgg
      481 taaaaacgaa gtttttgcca taatttccaa atacccaggc agtacggata atatagaaat
      541 tagacacatg cttctcaact aactcttccc ccatacgctt agtgcgtcca tattctgtct
      601 gtggatctgg tcggtcatca acttcccact cttgtccaac tggtttctta ccgtcaaaga
      661 cctagtccgt agaaatataa actagagttg caccatgctt ttcagatgct tttgcgacat
      721 tttttgtccc catcacattg atggcgaagt ccaactcttt tccttcatcc tctgctgcat
      781 caacagcggt gtaggctgca caatggtaga ctaaagtcgg tttcacctct tcaaaaactt
      841 tctcaaccat ttcttcattg gtaatgtcca tctcagccac atctactgcc acgtattctt
      901 cattacgttc atccaataaa tagcgaagtt ccgttcctaa ttggccattt gcccctgtaa
      961 ttaaaatcat ctctagacct ttctaatatg taactcttcc caatatagca ataaaaagca
     1021 gcgatttcct gctttttata ctgtaataat ctcctgagtc ttagcataat tggcttcaac
     1081 agcttctttt tctgctttcc accattcttg gttatctgta taccacttga ttgttgcctt
     1141 gagcccagct tcaaagttgg taaattcagg tttccacccc aactcatcac ggagcttgct
     1201 ggcatcaatc gcatagcgaa ggtcatgtcc tgcacggtca gtcacatgat cataggcatc
     1261 cgtagcttgt cccatttcct taaggataag ttccaaaact tccttattgt tcttctcacc
     1321 atcagcccca atcaagtagg tttcaccgat ttgccctttt gtcaagattg tccaaactcc
     1381 tgaagaatgg tcattggtat gaatccagtc acgaacgttc ttaccttcac cgtaaagttt
     1441 tggcttaatt ccacttagga tgttagtaat ctgacgtggg atgaattttt cgatatgttg
     1501 ataaggaccg tagttatttg aacagttgga aatcgttgcc ttgactccaa aagaacgcac
     1561 ccaggctttg acaatcaagt ctgatgcggc cttggttgat gagtacggag agcttgggtt
     1621 gtattttgtt tcagcagtaa atttctcacc tggtccttca ccatgacctg gcaaatcttc
     1681 tcgtaaaggg agatccccat aaacttcatc tgtcgataca tggtggaagc gaatatcata
     1741 cttacgagca gcttctaaaa gagtatagat tccaatgaag ttagtatgaa taaatggcga
     1801 tggatcattg agcgaattat cattgtggct ttccgctgca taatgaacga tagcatctgc
     1861 ttgagcaacc aacttgtcta ccaactccgc atcagcaatg tcaccaacaa ctaactcaac
     1921 acgattgcct aaaatttcct caatattcgc gcggtttcca gcataagtca acttatctag
     1981 gactgtcacg tgaacatctg gaaagttctc gtaaacataa tggacaaagt tagaaccgat
     2041 aaagccagct ccacctgtca cgataatatt tttgtattca gtcatattct ttccttattc
     2101 caaatcttct tttttcaaag gcttcacgtc cttaagtagg ggatgatgtt tgtctgcttc
     2161 agatacgtct gcttcttcca gattttccca ttcaatacca aggcttggat cagcgtagtt
     2221 cacaaaggca tacttgggtt tgagttcaag agcccagtaa tcattgacca gatagctata
     2281 tgacactgta tctgataaaa cttggaagcc attagctacg cctcgaggaa caaagattcc
     2341 cttgcttgca tcaattactg tctgataggt attcccaaag gtttcaccct cgcgtagatc
     2401 aacccaagaa cccagaactt tccctccatc tgctacagag atgtacttat cccaaggctc
     2461 tgcgtggagg cctcgaagga catttttacg tgagaaggat acattgtttt gcaattttcc
     2521 ttctgcaaag aaagactctg gaaatccaag tggaagcact ttttcctttt ggaaattttc
     2581 tttaaaccag ccacgattat ctccatgaac ggggatatca aactccaaca tgcctggaat
     2641 agcttcaacc ttgcgtgccg caagcgtctt accgaaaaaa ttatctgtca tctatgcttc
     2701 tccaatcaaa cggagcagat actgtccgta ttcatttttc ttaagtggtt gggctaaggc
     2761 caatacatct tctcgactga tatagcccat acggtaagca atttcttcta agtttgctac
     2821 ctgaacattt tgcatccgtt gcactgtttc gatgtactgt gaagcctcta gtaaactttc
     2881 atgagttcca gtattcagcc aagcaaagcc acgtcccata agctcaacag acaaatcacc
     2941 acgatctagg taagccttgt ttacatctgt aatttccagt tcaccacgag ggcttggttt
     3001 aatactcttg gcaatttcga caacatcgtt atcatagaaa tacagtcctg tcactgcata
     3061 gttggagcga ggatactccg gtttttcttc tatggaaata gcattcatgt ctgtatcaaa
     3121 ctcaaccaca ccaaaacgct ctggatcctt cacttggtag ccaaaaacag tcgcaccttt
     3181 ctctttcttg gctgcttttt gaagcattgt gctcagacca ggaccatggt agatattgtc
     3241 gcctaaaatc aaggcaacgc tatcgtcaga aataaactct tccccaattg ccaaaccatc
     3301 tgtacttggt tgctctgcat aagaaagttt gatcccaaac tcagatccgt cttgaagaag
     3361 ctcttgaaat cgatgtaaat cctgtggagt ggaaataatc aaaatatccc taatcccagc
     3421 caacatcaat gttgaaagtg ggtagtaaat catcggttta tcataaaccg gcataagttg
     3481 ttttgacgca gcgcgagtca aaggatataa acgtgtcccc gaaccacctg ctagaataat
     3541 acctttcata gtagggtacc tttctttgtt attttagagt caattactat taattgtgta
     3601 ctgctctaaa aagaaaacaa tcatcccatg ttttatatgg ctctataata tttgtagtgg
     3661 gtaaatccac tatagatatt atggagccta ttt
//
LOCUS       DAVBCV010000098         3365 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBCV010000098 DAVBCV010000000
VERSION     DAVBCV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.098"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            513..1859
                     /gene="gdhA"
                     /locus_tag="VQ516_002041"
     CDS             513..1859
                     /gene="gdhA"
                     /locus_tag="VQ516_002041"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2512419.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2365..2535)
                     /locus_tag="VQ516_002042"
     CDS             complement(2365..2535)
                     /locus_tag="VQ516_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512420.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2548..3126)
                     /locus_tag="VQ516_002043"
                     /pseudo
     CDS             complement(2548..3126)
                     /locus_tag="VQ516_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3143..>3365)
                     /locus_tag="VQ516_002044"
     CDS             complement(3143..>3365)
                     /locus_tag="VQ516_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512421.1"
                     /translation="QAQTPRKSSKLKPLTAEDKACNHVLSKERSKVENIFAKVKTLKM
                     FSTTYRNHRKRFGLRMNLIVGIINHELGF"
BASE COUNT         1046 a          616 c          565 g         1138 t
ORIGIN      
        1 cacctctact tctaaaacat tgttagaaat cgatttgact gtcctaatcg atttgtcctg
       61 ttcttgtttc attttactat agaacgattt gaaggcgttt atgatattta gctgtacgag
      121 agtcttttaa aagtgttttg atggcttgga tttcttcttt agttgatttc atatcactat
      181 tatataatga tttttgattt tagtctggta taaatattgc tttcctccaa aatggtcata
      241 attttactag caaatctaac atatcacgga taaattaaca agtgatttct gaattgctaa
      301 acattttctt ttcttatagc ataccatact ttaagatttt gtctttgaga aagatatttc
      361 caagaaaaac gttcgttttt ttatctattt tcaaaaaaac caaaaactga attgaaagaa
      421 tttttagaaa atttctgttt tttctttaca gtgaaaaaaa attctgctat aatgtcttat
      481 gtaataaaat atgacaataa aaggagaacg atatgacatc tgctaaagaa tatatccaaa
      541 gcgtgtttga aactgtgaaa gctcgtaacg ggcacgaggc tgaattcctc caagctgttg
      601 aagaattttt caacacttta gaacctgtat ttgaaaaaca ccctgagtat atcgaagaaa
      661 atatcttggc acgtattact gagcctgagc gcgtggtttc tttccgtgtt ccttgggttg
      721 accgtgatgg aaaaattcaa gtaaaccgtg gttaccgtgt tcaattcaac tcagctgttg
      781 gaccatacaa aggtggactt cgtttccacc caactgtaaa ccaagggatc ttgaaattcc
      841 tcggatttga acaaatcttt aaaaatgtct tgactggact tcctatcggt ggaggtaaag
      901 gtggatcaga cttcgatcct aaaggtaaaa cagatgctga agtgatgcgc ttctgccaaa
      961 gcttcatgac tgaattgcaa aaacacatcg gaccatcact tgacgtacct gctggtgata
     1021 tcggtgttgg tggacgtgaa attggttacc tttacggtca atacaaacgt cttaaccaat
     1081 ttgatgctgg tgtcttgact ggtaaacctc ttggatttgg tggtagcttg attcgtccag
     1141 aagcaactgg ttacggtttg gtttactata ctgaagaaat gctcaaagct aacggtaaca
     1201 gctttgctgg taagaaagtc gttatttcag gttctggtaa cgttgctcaa tatgctcttc
     1261 aaaaagcaac tgaactcggt gcaactgtta tttctgtttc tgactcaaat ggttatgtca
     1321 tcgatgaaaa tggtatcgac ttcgatcttt tggttgatgt taaagaaaaa cgtcgtgctc
     1381 gtttgactga gtatgcagct gaaaaagcaa ctgcaaccta ccacgaaggt actgtatgga
     1441 cttacgctgg aaactatgac attgctcttc catgtgccac tcaaaacgaa atcaacggtg
     1501 aagcagctaa acgtttggtt gctcaaggcg ttatctgtgt atctgaaggt gccaacatgc
     1561 cgagcgacct tgatgccatc aaagtttaca aagaaaatgg tatcttctac ggacctgcaa
     1621 aagctgccaa cgctggtggt gtagccgttt cagctcttga aatgagccaa aacagtcttc
     1681 gcctctcatg gactcgtgaa gaagttgatg gccgtctcaa agacatcatg acaaacatct
     1741 tcaacacagc taaaacgact tcagaaacat acggtcttga taaagactac cttgcaggag
     1801 ctaacattgc tgcctttgaa aatgtagcaa atgccatgat tgcacaaggt attgtttaag
     1861 atttatttac tcaatcctca atcactacga ttggggattt ttagcttgaa ttaaaaaatt
     1921 ggaaaatcta attaaataaa atgaaatgaa ataaaaactg aacaaattga ttgcggaatt
     1981 caaaccaatt tctaacatac ttctagaaat taatttgact tttctaatcg atttgttcat
     2041 atcttatttc aatttactat acttacttaa ccctttttat actcaatgaa aatcaaagag
     2101 caaactagga agcgagccgc aggctgtact tgagtacggc aaggcaaagc tgacgtggtt
     2161 tgaagagatt ttcaaagagt attaaacaaa aagaaaaacc gctactccta agaatagcga
     2221 tttaatcatg tttttaagct actactgtag ttgctctatt atttaagtgt tatgaataac
     2281 ttcaatatct ttaaagcttt ttgttagatt tataatttca actatagcca tctctactcc
     2341 ttagtttaca gcaaaaaatt atgcttaatt cttattgttt ttgtaaacaa tcagcgaacc
     2401 caatactatt ccattcaaca agcctaaaga aataccatat tcaactggaa aatctaaaaa
     2461 aattgcaaac actattccaa tcatcacccc tagtgaggct attgttaaaa ttacatattc
     2521 attttctttc ttcattttct ttacacctca tttccttttt gtatataagc tgtagtactc
     2581 tccaccaaga gttattaagt acttatgatt tcctgactca acaatcttac cacctttcat
     2641 tacaaaaata atatccgcat ccttaatcgt tgacaatcta tgagctataa ttatttgagt
     2701 acaaccctga ctttgtatat actttgttat tctttcctca ttaatagtgt ctaatgcact
     2761 agttgcttca tctaaaatta caatactagg attatttatt aatgcacgtg ccagtgctat
     2821 ctgttgcctt tgcccacctg aaatatttga ccccatctct gagatgatag tattaaattt
     2881 catcggcata gccatgattt catcatagat ttgaactgct ttacaaactt cctctatctt
     2941 ttgtgaagta acttcgtgct ttaaagttat attatcaaga atacttcggt tcaataaaaa
     3001 tgaatcctgt ggaactactc ctaaattttg acttaatatt tttttatcta tttgattaat
     3061 atttatacca taaaataaaa cttttcctgt atcaatctta tataatcctg atggtatttt
     3121 tgataataga cttcctgcaa aactagaatc ctagttcatg attgataata ccaacaatca
     3181 aattcatccg taatccgaag cgtttacgat gatttcgata ggttgttgaa aacattttaa
     3241 gcgtttttac tttggcaaag atgttctcaa ccttgcttct ctccttagat agcacatggt
     3301 tacaggcttt atcttcagct gttagcggct tgagtttgct ggatttacgt ggagtttgtg
     3361 cttga
//
LOCUS       DAVBCV010000099         3192 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBCV010000099 DAVBCV010000000
VERSION     DAVBCV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.099"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(381..914)
                     /locus_tag="VQ516_002045"
     CDS             complement(381..914)
                     /locus_tag="VQ516_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2512422.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ516_002046"
     CDS             complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ516_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2512423.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYKLKQRLTQVLARKGYDFSDIASALREYL"
     gene            1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ516_002047"
     CDS             1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ516_002047"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2512424.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1028 a          562 c          581 g         1021 t
ORIGIN      
        1 atttcatttt actatactgt tacgaaatta gatatataaa tgaaaacttg cctcaaggga
       61 ggttttttgc tagtagaaaa gcgatagaca gaaattgttg gactcttaaa aaaatgaagt
      121 tttgctcaaa agagcttctt aaattaaaac gagcaattat cagttcaaat ctgtttcaaa
      181 agaagaacgg ataaattaat cgatgctttg tttttgagtt aggaagttaa gcatgccaaa
      241 ctgagtcgta ggaataaatt tctttataga aatgtgttgt aactctgcta taatggattt
      301 attccttttt gtgtttacac aatttatttt atactaccaa aaaaacaagg tcaggatttt
      361 gttcctgacc tttgacaact ttaccgattc tttagttcta catagcgctt gtaccaaatg
      421 tttacatagg cttctgagaa aggaccacgt ccattgttaa tccaatcaac aagaattttg
      481 acatgttctt ttaaaatata gtccaagtca tcagaataat tcattttgcg tttgtgacgc
      541 tcgtactctt caacgtccaa gagacgtttt tccccatctg taaaaatttt aacatccaaa
      601 tcgtaatcaa tatacttcag tgcttcttca tccagatagt aggggctagc catattgcaa
      661 tagtaagaag ttccattatc acgaatcatg gcaatgatat taaaccaata tttcttgtga
      721 aagtaaacaa tagccggttc tcgagtgacc caacgacgac catcactttc ggtaacaagt
      781 gtatgatcgt tgacaccaat aatggcgttt tctgttgttt ttagtaccat ggtgtcccgc
      841 caagttcggt ggagactccc atcatgctta taactttgaa ttgtaataaa gtcgccttct
      901 tttggaagct tcataactaa ccaactttct acaatttata agtttatcat ttactattgt
      961 accataaaat tacctaaaat ctgtgaattt cacttggaaa tattaaagat attctctaag
     1021 agcgcttgct atatccgaaa aatcgtagcc ctttcgtgct aaaacttgag ttaaacgctg
     1081 cttcagtttg tatccttcat actttcgggc atacttagta tattgcttat caagttcctt
     1141 gaagatgagt tcctgagtcg tttcttcatc aacttgacta tccaattcgt caaaggcaat
     1201 tttagcatca aaataagaga agcccttgtt agtcaagttc tggataatct tatcttgcag
     1261 ggcacgagct ggaagttttc cctcatattt tttcaatagt ttattggcta cacgttgagc
     1321 aacttccgaa aaatcaaaat cattcaagat ttcttctata gtagattttg aaattccttt
     1381 ttgtgctaat ttctgagtca gtacataagg tcccttgtct cctgaaagtt gattggcatt
     1441 gatgatagca taagcgtact gaccatcatt aatccactta tcttctttaa gattagcaat
     1501 aacttgagaa acgatgtttt tatcaatatc gtattttttc agatattctc tgacttcttt
     1561 ttcagtgcgt gctttaaagg ataagtggta gagggccaga ttcttaccat aagaaaattg
     1621 agcaaagtct tgaatctctt tcaattcctc ttcgcttatc accttatctc tcgataacat
     1681 aaaacgaaca attgtatctt cggtgatata gcatttgtcg ccattatcaa gctccatcag
     1741 atagagtctt tttttctttt caagttttgt gattttcata gctctattat aactcaaaat
     1801 gtgataagat aggggtatga atctgaaagt gaaacaaaaa ataccattaa aaatcaagcg
     1861 catgggaatt aacggtgagg gaatcggctt ttaccaaaaa acattagtct ttgtaccagg
     1921 agctctcaaa ggcgaagata tctattgtca gattacttct attagacgca actttgttga
     1981 agcaaaatta ctgaaggtca acaagaagtc taaatttcga attgtgccat cttgtactat
     2041 ttataatgaa tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt
     2101 caagacggac ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta
     2161 tgaaattcgt ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt
     2221 tcagactcga aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta
     2281 tttagtagag ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg
     2341 cttagcagaa ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg
     2401 tgtccgtact attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt
     2461 tacaaaccgc cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga
     2521 agttgtgaca gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa
     2581 gacagagatt atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc
     2641 actatcccct cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga
     2701 agcagtaaaa gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt
     2761 tggaacgatt ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat
     2821 tccagaagct attgaagatg ccaagcgaaa tgctaaaaga atgggatttg acaatactca
     2881 ttatgaagct ggaacggcag aagagattat tcctcgttgg tacaaggaag gctaccgagc
     2941 agatgctttg attgttgacc caccacgtac aggtctggat gataagttat tagatactat
     3001 tcttacttat gtaccagaaa aaatggttta tatttcttgt aatgtttcga ccttggctcg
     3061 tgatttggta cgcttagtag aagtctatga tcttcattat atccagtcgg tcgatatgtt
     3121 cccacataca gctcgaactg aagctgttgt aaaattaata aaaaaagttt aaaaaaggtg
     3181 ttgacaaaac at
//
LOCUS       DAVBCV010000100         2990 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBCV010000100 DAVBCV010000000
VERSION     DAVBCV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.100"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            101..1078
                     /locus_tag="VQ516_002048"
     CDS             101..1078
                     /locus_tag="VQ516_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2512425.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGVRFEAEK"
     gene            1075..2157
                     /locus_tag="VQ516_002049"
     CDS             1075..2157
                     /locus_tag="VQ516_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2512426.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFELLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(2221..2990)
                     /locus_tag="VQ516_002050"
                     /pseudo
     CDS             complement(2221..2990)
                     /locus_tag="VQ516_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          926 a          666 c          534 g          864 t
ORIGIN      
        1 aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt
       61 tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat
      121 cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca
      181 actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca
      241 gaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta
      301 tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa
      361 acatgttatt cttgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca
      421 aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattatc acgagaaagc
      481 ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta
      541 tgccaaatac tcttccaaga tgcctgactt attggctgga caaacaccaa atgtcttttc
      601 agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt
      661 tcgtcttttt gaaaaagcta atgacgcgac ttaccatgct caacagcttg acaatagcat
      721 tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg
      781 aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct
      841 caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca
      901 gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat
      961 gatccagcaa ccagatctga atctctacca aacttggctg tatgatgcag gctctgttca
     1021 tgagctacta tataccatgc gccagactgc tggcgttaga tttgaggcag aaaaatgaaa
     1081 accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc
     1141 cccattcaaa ctcaactatt tgagctcctt cttgctggag aaaacctcct aggagtgagc
     1201 caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag
     1261 aaaaaagccc aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt
     1321 gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt
     1381 tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct gattggaact
     1441 cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc
     1501 atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt tgtagagaaa
     1561 atcactcact acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt
     1621 gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgatca aaaattggac
     1681 aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat gctacgaaaa
     1741 ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg
     1801 aatgccgagg aaaaactaca gtatcgtgat atattggctg tttccctcgc tagtgatgtt
     1861 aatgttaaat ttagaaaaat catcttagaa aagtttaaag ataaccaact aaccctgctt
     1921 cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac
     1981 tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac tggtcgtatg
     2041 ggcaaggaag gttatgttat cactctcgtc actcatccag aagaaatcaa aaaacttaaa
     2101 aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc
     2161 cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc aattttatac
     2221 tcatctgctt tcaaaaagca ttctagtcca tctccgatta acgatggact ttatcacctc
     2281 cttctccaat ccttgtatga catcttgaag ttgattcatg acatcttcca aaattcgaaa
     2341 ggctttattc ttaaatccac gtttacgaat ctctttccac acttgttcaa tggggttcat
     2401 ctctggtgtg tatggaggaa taaatgcaaa accaatatta gtcggaatct ttaagatact
     2461 tgatttatgc catatagcat tgtccataac gagtaaaaaa taatcatctg gataagcttg
     2521 tgaaagcttt tctaaaaagg cgttcatcca ctcagtatta catctaccag ctattaagaa
     2581 aaatgattcg cctgtatggg catcaacagc tccataacaa tagcgaaatt ctcgtatata
     2641 gtgactatgg atatgtggac ctactcctat tggagcccaa caagatccca gtttactgat
     2701 tctaccgaaa ccagcctcat cttggtacat caagcgaacc ttacgaaaac gtctactata
     2761 tttaaaacgc tttcttgtct tcttgaattg agattttatt tttagacgcg acaatggttt
     2821 gagcgtctgc tttcttagga tgttctggac gtggcataat atttcgccaa ccatggcgct
     2881 tcaacagttg atagaaggca tcacgtgtgt aggaacgacc taactccttt ttataagcct
     2941 gaaataaggc atcaattgta acaaattctc ctgcctctgc agccttcaaa
//
LOCUS       DAVBCV010000101         2878 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBCV010000101 DAVBCV010000000
VERSION     DAVBCV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.101"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(384..1859)
                     /locus_tag="VQ516_002051"
     CDS             complement(384..1859)
                     /locus_tag="VQ516_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2512427.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            2566..>2878
                     /locus_tag="VQ516_002052"
     CDS             2566..>2878
                     /locus_tag="VQ516_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2512428.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTKIKRRTVRKCLGKGRFKEVYSADYAQQSYENNRKHSVKRSSVTKELKEKILHYHNQ
                     KF"
BASE COUNT          904 a          624 c          519 g          831 t
ORIGIN      
        1 ataagaaaga cgctcatggc atcgctctta aacttctttc tgaccccaat cgtgaacaat
       61 ttcaacacga taatcggcaa gtagaactaa aaatactgac tagacatatt catcgtctca
      121 agaaaaaaca gtctgattgg aaagtacaat acactcgttg tcttgatatc atctttcctg
      181 agttggataa aatcgttgga aagcattcag aatataccta ccaactcttg acacgctacc
      241 ctaatcctca gaaaaggatt gaggcaggat ttgataagct gatagaaatt aagcgattga
      301 ccgcttctaa ttcaagggct ttttgccgtg ctctaaaatc atcgccaaat catcaaattg
      361 aaataagaaa tagtttagat aaactattga cttttcttat agtttgtctt tctcttaata
      421 acgttgatat gattgacctc tggatcttcc gtcccttgga gggagcaacc acgttccttg
      481 gcgaattgga tatagtcgac aatttctcgt gtaatgcgtt caccaggagc caagatagga
      541 atacctggag ggtaacacat aacaaattct ccacagacct gtccaacaga atcatccaaa
      601 gttaaacttt ttctttctga atagaaggct tcttgcggag acagcactaa ctcgggctga
      661 atatattctc ctgctatcaa atcttttcca tctcttgaat agagtctctt aatatcagcc
      721 agagcaccaa ccaagcgctc gatgtcttgg atgcggtcgc cgatggaaat ataggccaag
      781 atattgccga tatcaccaaa ctcgatctga atgtcgtatt cgtctcgcaa gaggtcataa
      841 acctcgatac ctgttaagcc aataccctga gtgtaaactg acaacttagt tacgtcaaaa
      901 tcgcaaaccg aaacaccgtc tattaactct tttgagtagg catagtagcc accgatagca
      961 ttgatttcat gacgagcgta ctcggatagc tcaatgactt cctcaaacga ctctttacca
     1021 cgaagggcca agttgcgacg tgaaatatcc aaactagcca tcaataagta agaggcagat
     1081 gtagactggg tcaggtttat aatctgacga acgtactcgg gattcatctg ctccccgatt
     1141 aaaagaagcg aactttgtgt caaactccca ccagacttat gcatggaaac tgctgccata
     1201 tcagctcctg catccatagc agaaattgga agtttaccag tgaaatgcaa atgcgctcca
     1261 tgggcttcat ctactaaaac catcatgcca gcttcatgag ccatttctgt caaccccttt
     1321 aggtctgaac agatgccgta gtaagtagga ttgttgatta ggatagcctt agcatctgga
     1381 tggtccttta tggcctgtgc tactcggtca ttttcaagac ctaaagcgat accaatctta
     1441 ggatctacac tcatctcgat atagatggga atggcaccac atagaaccaa cgcattgata
     1501 gcagatttat ggacatttcg tggcagaata atcttatctc ctgccttgca ggttgccaga
     1561 atcatagtct gcaccgatga agttgttcca ccaatcatta gaaaggcatg gctagctcca
     1621 aaagcatctg cagccagctc ctctgcatca cgaataatcg aaataggatg gcctaaatta
     1681 tccaagggtt tcatcgaatt gacatcaatg cctacacatt tttctcctaa gagttcgaca
     1741 agttctggat ttccccgtcc acgcttgtga cctggaacat caaagggaac aatccttttc
     1801 ttgcgtaact tcaccaaggc ctcataaatt ggggcttggt tttgatctaa ctctttcaaa
     1861 acatactcct ttcaatattt ttaggacctt atgaaaacta aggaactaaa ggttgactca
     1921 tgaaaaaaga gcaggttttc acctgctctg caatcttctg acgtgtttat tttggtttct
     1981 gttgagtatg tctgttcccg atgtttccta actctctagt agcaaatatt acgtacttta
     2041 ttgatcgttt cacaagatga cgtgtgcttt caccacacta aaaatttatg aattaggaca
     2101 agtgtcctaa catcaactta taaaagtagg cttttaaccc tatcaataat gtggcttttc
     2161 aaccacgctt cggcattcaa ccctgcctgt ccgtaggcat tttttactcg ggtttatatt
     2221 tgctagaaaa catcatctca ttttctaagc tttattacta tatcatgttt ttaagaactt
     2281 gtaaagtatt ttttgataaa aaacaaaaat ataatagatt gaaactagaa tagtacacct
     2341 ctgcttctaa aacattgtta gaaatagatt ttactgtcct gatcgatttg ttctgttctt
     2401 gtttcatttt actatatgtt cgtaaatgaa agttctataa tgaagttagc cacccagccc
     2461 tcctaaaaat tagatggttc aactagctga tagctcttgt aggcataaga aaacacctct
     2521 gtgttatact tgttgttcaa ccacaaacac aagaaaggca cagaaatgca agacaattat
     2581 actacaaaag gtaaacattt gacaatcgat agccgtcgct taatcgaaag atggaaaaaa
     2641 gaaggaaaat caaatagaga aattgcctct ctacttggaa aagctcctca aactatccac
     2701 actaaaatca agcgtaggac agtccgaaaa tgtcttggaa aagggcgctt caaagaggtt
     2761 tattctgccg actatgctca acagtcttat gaaaataatc gcaagcactc ggtcaagaga
     2821 tcaagcgtga ccaaggaact aaaggaaaag attctccact atcataacca aaaatttt
//
LOCUS       DAVBCV010000102         2713 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBCV010000102 DAVBCV010000000
VERSION     DAVBCV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.102"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(50..244)
                     /locus_tag="VQ516_002053"
     CDS             complement(50..244)
                     /locus_tag="VQ516_002053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512429.1"
                     /translation="MSSKKHPLGEKRNHTGSKLRQGFWMVFRLGVNNWGFKEIENKKP
                     SKNSIAGENEIILNENQRAN"
     gene            306..1652
                     /locus_tag="VQ516_002054"
                     /pseudo
     CDS             306..1652
                     /locus_tag="VQ516_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
     gene            complement(2052..>2713)
                     /locus_tag="VQ516_002055"
     CDS             complement(2052..>2713)
                     /locus_tag="VQ516_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512430.1"
                     /translation="IIRKVQEPEFWKEVNLSDLEKIRLAIRDLLQFLDKTDRKPYYVN
                     FEDRILSTVHETTAFLQVNDLRSYNEKVEHYLKTHLDEESISKLYHNKKLTSDDMLAL
                     EKLLWEKLGSKADYQSHYENKAIPRLVREIIGLDRESANRIFSKFLSDENLNARQISF
                     VKLIVDYIVENGFLETKVLTQEPFKSYGSVQLLFQHQLPVLRNIVQIIELINNRAGEA
                     A"
BASE COUNT          816 a          561 c          482 g          854 t
ORIGIN      
        1 cttatctaca acctcaaaac agtgttttga gcagcctgca gctagtttcc tagtttgctc
       61 tttgattttc attgagtatt atttcatttt ctcctgcaat tgaattcttg ctcggctttt
      121 tgttttctat ttctttaaaa ccccaattat tcacccctaa tctaaaaacc atccagaatc
      181 cttgccttag cttagatcct gtatggtttc ttttttcacc caatgggtgt tttttactag
      241 acaaaaaaga atttcccctt tatggtataa gtgtagaaaa aaacacaaaa agaaaggaaa
      301 ctcacatgaa cagtttacca aatcatcact tccaaaacaa gtctttttac caactatctt
      361 tcgatggagg tcatttaacc cagtatggtg gtcttatctt ttttcaggaa cttttttccc
      421 agttgaaact aaaagagcgg atttctaagt atttagtaac gaatgaccaa cgccgctact
      481 gtcgttattc ggattcagat atccttgtcc agttcctctt tcaactgtta acaggctatg
      541 gaacggacta tgcttgtaaa gaattgtcag ctgatgccta ctttccaaaa ttgttggaag
      601 gagggcagct tgcttcacag ccaaccttat cccgttttct ttccagaact gacgaggaaa
      661 cagtccatag tttgcgatgc ctcaaccttg aattggtcga attcttttta cagtttcacc
      721 agctaaacca actcattgta gatatcgatt ctacccattt cacaacttat ggcaagcaag
      781 aaggtgttgc ttataacgcc cactatcgtg ctcatggcta tcatcctctt tatgctttcg
      841 aggggaagac aggttattgt ttcaatgccc agcttcgtcc tggtaatcgt tattgttctg
      901 aagaggcaga cagctttatc acacctgttt tagaacggtt taatcaactt ctctttcgaa
      961 tggatagtgg ctttgcgacc cccaaattat acgatttaat tgaaaaaaca gggcaatact
     1021 aactcataaa actcaagaaa aatactgttc tgagccgtct tggagacctt tccctccctt
     1081 gcccacagga tgaggactta accatcttgc cccactccgc ctactcagaa actctctatc
     1141 aagcaggatc ttggtcgcac aagcgtcgtg tctgccagtt ctctgaacga aaagaaggaa
     1201 acttgttcta cgatgttatt tctctcgtta caaatatgac gagtggaaca agccaagacc
     1261 agtttcagct ttatcgtgga cgtggtcaag ccgagaattt catcaaggag atgaaggagg
     1321 gattttttgg cgataaaacg gatagttcaa ccttaatcaa aaacgaagtt cgtatgatga
     1381 tgagctgtat cgcctacaat ctctatcttt ttctcaaaca tctagctgga ggtgacttcc
     1441 aaactttaac aatcaaacgc ttccgccatc tttttcttca cgtggtagga aaatgtgttc
     1501 gaacaggacg caagcagctc ctcaaattgt ctagtctcta tgcctattcc gaattgtttt
     1561 cagcacttta ttctaggatt agaaaagtca acctgaatct tcctgttcct tatgaaccac
     1621 ctggaagaaa agcgtcgtta atgatgcatt aaagaacagt cgagatgaaa aaatcgtgtg
     1681 acgcaccaag ggaggagtct gcccttttga ggaaatctag cgaggaaaaa cgatactgga
     1741 acagcagaaa gtaaaactga cctcatgagg aggaagaaag tggctcatga ggtcaggggt
     1801 tttgtaagtt acatctagtt gagagaggta tgaatgattt gggttaaaat caaagtagct
     1861 cttttgttaa taactcgatc aacaaacatc gtggtacaag tatctacttt gaaatttatc
     1921 aaccacttaa caactgatac tgtattctag gaaaacgatg acattcttcc taataaaact
     1981 tctcatatat agcataaatt tctactcttt ttaattttaa atgagtctca gcatggcaat
     2041 cactttagaa tttaagccgc ttctccagct cgattattga taagttcaat gatttgaaca
     2101 atattacgaa gtactggtag ttggtgttgg aagagtagtt gaacagaacc ataagattta
     2161 aacggctctt gcgttaacac tttcgtctct aaaaaaccat tttctacaat gtagtctaca
     2221 atcaatttta caaatgaaat ctgcctggca ttaagattct catccgacaa aaatttagag
     2281 aaaatacgat tggcagactc tctatctaag ccaataatct cacgaaccaa tctcggaatt
     2341 gccttatttt cataatgact ttggtagtct gctttactac ctaatttttc ccaaagcaat
     2401 ttttcaagtg caagcatatc atcagatgtc aactttttat tatggtatag cttagaaatg
     2461 gactcctcat ccagatgagt tttcaaataa tgctcaactt tttcattgta agaccgaaga
     2521 tcgttgacct gcaaaaatgc tgtggtctcg tgaacagtgg agagtatacg atcttcaaag
     2581 ttaacatagt agggtttacg gtctgtttta tccaaaaact gtaataaatc tcgaatagca
     2641 agacgaattt tttccaaatc agacaagtta acttctttcc aaaattcagg ctcctgtact
     2701 ttcctgataa ttt
//
LOCUS       DAVBCV010000103         2613 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBCV010000103 DAVBCV010000000
VERSION     DAVBCV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.103"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            195..509
                     /locus_tag="VQ516_002056"
     CDS             195..509
                     /locus_tag="VQ516_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512431.1"
                     /translation="MWVLGFILFLIFFYSNDSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            519..725
                     /locus_tag="VQ516_002057"
     CDS             519..725
                     /locus_tag="VQ516_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2512432.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            725..922
                     /locus_tag="VQ516_002058"
     CDS             725..922
                     /locus_tag="VQ516_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512433.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            978..1658
                     /locus_tag="VQ516_002059"
     CDS             978..1658
                     /locus_tag="VQ516_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2512434.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVVIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            1670..2311
                     /locus_tag="VQ516_002060"
     CDS             1670..2311
                     /locus_tag="VQ516_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2512435.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2329..2559
                     /locus_tag="VQ516_002061"
     CDS             2329..2559
                     /locus_tag="VQ516_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512436.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          763 a          427 c          539 g          884 t
ORIGIN      
        1 aaggtgaagc tgacgtggtt tgaagagatt ttagaagagt attaatgata gataaagtgg
       61 tcaggaacct actcctgacc tttttctttt gcaaaatgac aaaaatcata atttttttga
      121 caactatact tgtcaaaaag aaaaagatgt gttacaatgt aagcaagtta agagaaaaga
      181 agaaaggagc tatgatgtgg gtactagggt ttatactatt tctgattttc ttttattcta
      241 atgattctaa aaaaatcaag aaactagaga agaaaatcaa aagacttgag cgaaaagaga
      301 aaggaaatgc agaaatgtcg agattattac aagaaatgat tggaaaggaa ccaattataa
      361 cgggagtgta tattgggcca gataactggg aagttgtgga tgttgatgag gaatgggtaa
      421 agctacgacg tgtagataat acgggaaaag aaaaattcaa gttgcaacgt attgaggata
      481 tccaaaccgt tgaatttgac ggagagtagg tgtgtaacat gcaactaaaa aatcgtctaa
      541 aagagcttcg agctcgcgat ggtctcaatc aaaccgacct agccaaactg gcaggggttt
      601 ccagacagac cattagccta ctagaacggg atgagtacac cccatccatt atcattgccc
      661 tgaaaatatc tcaaattttc aacgaaacag tcgaatcggt atttcgtttg gaggaggacg
      721 agtgatgaac aagtataaag tgatttatta tgtagtggtc atagctttat tagtcagcgt
      781 atttctactg attgggatgg acctaagctg gtttaatccc tatcaaagcg accaatttgt
      841 ttgggtctac tttgctctca tcccagtaat tgaatggatt gaaaagaaat ccaaaaatct
      901 agcaagtgaa aaaggagaat gaatatgaaa aagaaacgta gattgttatt tttgatgtct
      961 attgtcttgg ggggttcttg ggcatgtttg tagggatgtt taaggcacgt gtcgaatccc
     1021 acgaaattat tttagatgta aaagccttga tgccatggat atcagctatt tgtttactga
     1081 taggtttcat tagtatgttt ttgactttca atttcttaaa gaaaagcaga aaatttcatt
     1141 ccttgtatca agaggaaatg gatgacgatc tgaatgaaac ctattatgtg caaatgtatc
     1201 ggaatcttga gtttggaacc attgctttta atattacagg tgtagtgatt ccattggcta
     1261 tttttatttc attaagtgag gtgattatat tgcatacaaa ccctcaaaca tttttccttt
     1321 ctttcttact ctttgtggta ttcttagtcg ctcaaaaatc tctttttaaa accattgcga
     1381 ttgttcgtca gtttgatttg gaatttttcg ctacaccaaa ggatgtcttg aactatataa
     1441 attcttatga tgaaggggag cgtcaggcta atttggaaca gagttttcga attttattcc
     1501 aattacacca atatgtctta ccagccttat atatttttct tattatcctt tctttcttga
     1561 caggagagat tcagttacta gctttcttgc ttgtaggagc catccatgtt tatatcaatg
     1621 tgatgcagtt acctatggta aaacgttatt tcaaataaag gagttatcta tgaaattact
     1681 taaaaacctt ggctggtttc ttctagccct tctatccttt ttctttatct atggctttat
     1741 tcaggggctc gcgactgcgt cgcttgcttt aggcgcttca ccctatgctg ttactctact
     1801 ctatgtggtc ttggctggag tatatgtgta cggcatttac aaatggtatc agaaagctcc
     1861 tgtttatatt gagaagagcg gctttaaccg atttatttgg cttcctgcct tggtttggtt
     1921 cttatctctg gtcgttcaat tcttcttgcc agatgatcct tcagtaaatc agcaaatagc
     1981 gacagacttg accttgtctc aaccactttt ctcattcttt gctgtggtta tttttgctcc
     2041 tttgacggaa gagattgttt ttagagggat gttagcacgc tatctctttc ctaagcagga
     2101 caatagtaaa cgaaccctga tttttcttct ggtatctagt cttctatttg ccttgattca
     2161 ttttccaggt gatgtgcaac aattttttgt ctattttagc cttggtttta gtttgggttt
     2221 ggcttacatt agcagaaaag gtctggtcta cagtatttct cttcacgctt tgaataattt
     2281 agtcggcttt ttgatgattc tcatgctata atagagtcag gaggtcacat gaaacgagta
     2341 attttattag cagtgattca ggcagtcgtt ctatttttca tcattggagc gctagcttat
     2401 gccttcaaag gcgatttctt ctataactat ctagcagttg tctttgctcc tattgcaggt
     2461 gtactgcgtt ttgggacggc ttacataacg gaaattgtct tgcctcgaaa ggcagccgaa
     2521 atcgctgaaa agcgtaaagc aggcaaaaat tcaaaataaa agagtccggt gaagttcatc
     2581 ggatttttgt attatactca atgaaaatca aag
//
LOCUS       DAVBCV010000104         2312 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBCV010000104 DAVBCV010000000
VERSION     DAVBCV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.104"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(1..410)
                     /locus_tag="VQ516_002062"
                     /pseudo
     CDS             complement(1..410)
                     /locus_tag="VQ516_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(355..537)
                     /locus_tag="VQ516_002063"
     CDS             complement(355..537)
                     /locus_tag="VQ516_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2512437.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            872..>2312
                     /gene="pcpA"
                     /locus_tag="VQ516_002064"
     CDS             872..>2312
                     /gene="pcpA"
                     /locus_tag="VQ516_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET2512438.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDGA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGS"
BASE COUNT          814 a          345 c          379 g          774 t
ORIGIN      
        1 catttctttt tacttccgtc acatcaatca ttaccgtgtc ctcagaactg agaggagttc
       61 ttgaaatcgt aacaccacct ttgaacaaga gttacttcaa cccattagct ccgacggatt
      121 aagttgcttt cgtgaatacc aaaatcagcc gcaatttctt cataagtgcg gtattctcgc
      181 acatattgaa gagtggccat aagaaggtct tctaggctta atttaggttt tcgtccacct
      241 tttgtgtgtt taagttgata agctgttttt aacacagcta acatctcttc aaaagtggtg
      301 cgctgaacac caacaagacg cttaaatcgt gcatcggtta gttgtttact tgcttcataa
      361 tttcgcaggg agtctattga ctctttggta ggtgtcaatg tttttttcat ctatcccgag
      421 aattattttc ccgccatttg tatttgcaaa tgctgagtag gtttcccaga aagactctgg
      481 aagattgttt ttagcttttt tgtattctaa atcaacccct tcaaatttta agtccatatt
      541 tttcctttac atctgttttt tgtggttctg gtatttgttc aagttgagtg ataatatagc
      601 gaattgaatt tcgagagttt ttactcagtt aatttctttt ttaacccact ttaatttctt
      661 ttttaacacg ggttaaaaaa gaaattaaag tgggttaatt tttcttgact taaaatttaa
      721 aaatgatata ctgtgtttat ggagagatga caatttataa aatgtactac tctattctac
      781 tagataatga ttgaataaaa ctttttataa caacggctag caaagttata attttatatc
      841 tattagaaaa taaatatgta aggaaattta aatgaaaaaa actacaatat tatcattaac
      901 tacagctgcg gttattttag cagcatatgt ccctaatgaa ccaatcctag cagatactcc
      961 tagttcggaa gtaatcaaag agactaaagt tggaagtatt attcaacaaa ataatatcaa
     1021 atataaggtt ctaactgtag aaggtaacat aggaactgtt caagtgggta atggagttac
     1081 tcctgtagag tttgaagctg gtcaagatgg aaaaccattc acgattccta caaaaatcac
     1141 agtaggtgat aaagtattta ccgttactga agtagctagt caagctttta gttattatcc
     1201 agatgaaaca ggtagaattg tctactatcc tagctctatt actatcccat caagcataaa
     1261 aaaaatacaa aaaaaaggct tccatggaag taaagctaaa actattattt ttgacaaagg
     1321 cagtcagctg gagaaaattg aagatggagc ttttgatttt tctgaattag aagagattga
     1381 attgcctgca tctctagaat atattggaac aagtgcattt tcttttagtc aaaaattgaa
     1441 aaagctaacc ttttcctcaa gttcaaaatt agaattaata tcacatgagg cttttgctaa
     1501 tttatcaaat ttagagaaac taacattacc aaaatcggtt aaaacattag gaagtaatct
     1561 atttagactc actactagct taaaacatgt tgatgttgaa gaaggaaatg aatcgtttgc
     1621 ctcagttgat ggtgttttgt tttcaaaaga taaaacccaa ttaatttatt atccaagtca
     1681 aaaaaatgac gaaagttata aaacgcctaa ggagacaaaa gaacttgcat catattcgtt
     1741 taataaaaat tcttacttga aaaaactcga attgaatgaa ggtttagaaa aaatcggtac
     1801 ttttgcattt gcagatgcga ttaaacttga agaaattagc ttaccaaata gtttagaaac
     1861 tattgaacgt ttagcctttt acggtaattt agaattaaaa gaacttatat taccagataa
     1921 tgttaaaaat tttggtaaac acgttatgaa cggtttacca aaattaaaaa gtttaacaat
     1981 tggtaataat atcaactcat tgccgtcctt cttcctaagt ggcgtcttag attcattaaa
     2041 ggaaattcat attaagaata aaagtacaga gttttctgtg aaaaaagata catttgcaat
     2101 tcctgaaact gttaagttct atgtaacatc agaacatata aaagatgttc ttaaatcaaa
     2161 tttatctact agtaatgata tcattgttga aaaagtagat aatataaaac aagaaactga
     2221 tgtagctaaa cctaaaaaga attctaatca gggagtagtt ggttgggtta aagacaaagg
     2281 tttatggtat tacttaaatg aatcaggttc aa
//
LOCUS       DAVBCV010000105         1822 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBCV010000105 DAVBCV010000000
VERSION     DAVBCV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.105"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..317)
                     /locus_tag="VQ516_002065"
     CDS             complement(<1..317)
                     /locus_tag="VQ516_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512439.1"
                     /translation="MILSKVRCYIYNSKIRGKVLYELYEFINENKQLMDAINSIKVDS
                     SNGFSIQILHLGEQQTTVLLTDFFTKTLDLFKEFRVTNKSTLEQVKNQISILNSSGPK
                     MDLT"
     gene            complement(330..1349)
                     /locus_tag="VQ516_002066"
     CDS             complement(330..1349)
                     /locus_tag="VQ516_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2512440.1"
                     /translation="MSNTLKQLLLFREEFIKNILNGIDVDETVVIENDFVEELENFLK
                     ENDLLDFSNSIFFQLSGDKVIVNNIYPGYLAYFNRFLTFYPKIFAIKEVGEYIDFLNF
                     EKRIADMFYCWGFNANSRVLTAEKIIEIPNHQINAKYIKKVIKMSELLFGVDSNNRIR
                     LLNNRRELIKTIFQGSVIKSAIPALAATLDTISNSGSFQISLSKQIINNLVLSGKDKV
                     TIPRIELDNVVLSRQKIIVSVSYINDFLDLRFPDKIEYILNFLEGSGFNTKVMVYFGK
                     IVEEDKNYFVNLEKPQYFDFTSILFNKLFFKELNKFDYMVIEELIPNLDEDVMMTEYI
                     KEYTE"
BASE COUNT          626 a          318 c          190 g          688 t
ORIGIN      
        1 gttaaatcca tttttggacc agaagaattt agtatgctaa tttgattttt cacctgctca
       61 agagtactct tatttgtaac cctaaattcc ttaaacaagt ctaaagtttt agtaaaaaaa
      121 tctgttaata agacagttgt ttgctgttct cctaaatgta atatttgaat actaaaacca
      181 ttactactgt caactttaat tgaattaatt gcgtccatca attgcttatt ttcatttata
      241 aattcatata attcatacag aactttacct ctaattttag aattatatat gtaacaacgt
      301 actttactta aaatcataat agattgctcc tattcagtat actcttttat atactctgtc
      361 atcattacat cttcatcaag atttggaatg agttcttcaa taaccatgta atcaaacttg
      421 tttaattctt taaagaataa cttattgaaa agtatactgg taaaatcaaa atactgaggt
      481 ttttcaagat taacaaaata gttcttatct tcttctacaa ttttcccaaa gtagaccatt
      541 actttagtat tgaaaccaga tccttctaaa aaatttaaaa tgtattctat tttatctgga
      601 aatctcaaat ccaaaaaatc atttatatac gaaactgata caataatctt ctgtcttgaa
      661 agaacaacat tatctaactc tatcctagga attgtaactt tatctttacc acttaatact
      721 aaattattaa ttatctgctt tgataaagaa atttgaaatg atccactgtt actgattgta
      781 tccaaagtag cagctagagc aggaattgct gacttaatta cagaaccttg gaaaatagtt
      841 tttatcaatt cccttctatt gttaagcaac cgaattctgt tattactatc aacaccaaac
      901 aagagttcac tcatttttat aacttttttt atgtacttag cattgatttg gtgatttggg
      961 atttcaatta ttttttcagc tgtcaacact ctagaatttg cattaaatcc ccaacaataa
     1021 aacatatccg caattctctt ttcaaaattt aaaaagtcaa tatattctcc aacttcttta
     1081 atagcgaata ttttcggata aaatgtaagg aacctattaa aatatgctaa atatcctgga
     1141 taaatattat tcactattac tttatctcca gataactgaa aaaatatgct atttgaaaaa
     1201 tctagcaaat cattctcttt taaaaagttc tccaactctt ctacaaaatc attttctatt
     1261 actactgttt catccacatc aatcccattc aaaatatttt ttatgaactc ctctctaaaa
     1321 agtagtaatt gcttcaatgt atttgacata tattcatctg gataatatgt ttccaaatta
     1381 acattagcga gtctaggagc ttctttgatg attatgttaa ctatatcctg aactatttct
     1441 tctgatgtcc ctaccttctg gtgttctctc ctgtgtttat tataataaca ttgtccgaat
     1501 agtagctgaa aaaacaaatt actatcaaat aatctcgtta gaattaatag ctttcttatt
     1561 gacttattat caacttttaa actttctttt tcaatttgcc tcaactctac aatgtcttga
     1621 taaataaaat tatgttctat aaaaatttac ttcaactagc tatggtttaa tagatttttt
     1681 aaaggggata attgtctaaa atttaacttt ttacaaatca agatgtctct ataaactagc
     1741 aacctctcta ataactttac ccctatagct atagacaatc gagtagggga taatctctag
     1801 cccctctcac accaccgtac gt
//
LOCUS       DAVBCV010000106         1651 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBCV010000106 DAVBCV010000000
VERSION     DAVBCV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.106"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            100..1575
                     /locus_tag="VQ516_002067"
     CDS             100..1575
                     /locus_tag="VQ516_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2512441.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          468 a          335 c          328 g          520 t
ORIGIN      
        1 tccagtattt gacaatattg aagcagctat taaagaacac ggattaagct aattctaatt
       61 tgttaacatt catataacat aaaaaaggga ggaactgtta tggatgcaat ctttgaccta
      121 atcggaaagg ttttcaatcc cattttagaa atgggtggac ctgtcatcat gttaatcatt
      181 ttgacagtat tggctttact ttttggagtg aaattctcca aagcgcttga aggtggtatc
      241 aaacttgcca tcgctcttac aggtatcggt gctatcatcg gtatgctaaa tggtgctttc
      301 tcagcatcac ttgcaaaatt cgttgaaaac actggtatcc aattgagtat taccgacgtt
      361 ggttgggcac cactcgcaac aatcacttgg ggttctgctt ggacactata cttcttgctc
      421 atcatgttga ttgtcaacat agtgatgcta gctatgaaga aaacagatac acttgacgtt
      481 gatatctttg atatctggca cttgtctatc acaggtctct tgattaaatg gtatgctgat
      541 aacaatggtg tgagtcaagg ggtttcactc tttattgcta cagcagctat cgtccttgtc
      601 ggtgtgttga aaattatcaa ctctgacttg atgaaaccta catttgatga ccttcttaac
      661 gccccaagtt catcaccaat gacatcaact cacatgaact acatgatgaa cccagttatc
      721 atggttttgg ataagatttt tgaaaaattc ttcccaggcc ttgataaata tgactttgat
      781 gctgctaaat tgaacaagaa aatcggtttc tggggatcta aattcttcat cggtttcatc
      841 cttggtatcg ttatcggtat tatgggaact ccacatccaa ttgcaggtgt tgcagatgca
      901 gataaatggc gtcttgttat caaaggatgg ttgtctcttg gtttgactgc cggtgtatct
      961 ttggaactct tctcacttat cggttcatgg ttcatcgcag ccgtagaacc actatcacaa
     1021 ggtattacaa acgttgctac taaacgtctt caaggacgta aattcaatat cggtcttgac
     1081 tggccattca tcgctggtcg tgctgaaatc tgggcttgtg ccaacgtact tgcaccaatc
     1141 atgttgattg aagcagtgct tctttcaaaa gttggaaatg gtatcttgcc acttgcaggt
     1201 atcatcgcta tgggtgttac tccagctctc ttggttgtaa ctcgtggtaa attgctccgt
     1261 atgattatct tcggaacact cttgttgcca ctcttccttc tttcaggtac acttattgca
     1321 ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc cagaaggtgt gagccaaact
     1381 caattgatta ctcactctac tcttgaagga ccaatcgaaa aacttcttgg ttggacaatt
     1441 ggtaacacta caactggtga tatcaaagca atccttggtg cagtagtctt ccttgtattc
     1501 tatatcggta tctttgcttg gtacagaaaa caaatgatca aacgtaacga agagtacgca
     1561 gcaaaagcaa aataatgcgc tcctataaaa tataaactca atgaaaatca aagagcaaac
     1621 taggaaacta gccacaggtt gctcaaaaca c
//
LOCUS       DAVBCV010000107         1576 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBCV010000107 DAVBCV010000000
VERSION     DAVBCV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.107"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQ516_002068"
     CDS             84..491
                     /locus_tag="VQ516_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2512442.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            656..955
                     /locus_tag="VQ516_002069"
     CDS             656..955
                     /locus_tag="VQ516_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2512443.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVNEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="VQ516_002070"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="VQ516_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2512444.1"
                     /translation="MMIVLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
BASE COUNT          541 a          313 c          280 g          442 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgtta atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
     1021 taacaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcagag
     1201 gaaaggtatt gtactatgat gattgtacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgaggca gggaatagac tcattagcct atgtagttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggctactat ataaacgctt tgagaacggc
     1441 agactgactt ggcccagtac agaaaaggat gtcaaagctc tcacatctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacctctact tctaaa
//
LOCUS       DAVBCV010000108         1529 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBCV010000108 DAVBCV010000000
VERSION     DAVBCV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.108"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            108..407
                     /locus_tag="VQ516_002071"
     CDS             108..407
                     /locus_tag="VQ516_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2512445.1"
                     /translation="MDSYAINFWESKGVGIDKKILEFIQKNITVIKNKNFLNKDFDIY
                     VKKIHNLFFVISTLVSKEQYMGIVSSQIHMFANRMGISPTIEVTMLDFFLREDSD"
     gene            409..1179
                     /locus_tag="VQ516_002072"
     CDS             409..1179
                     /locus_tag="VQ516_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET2512446.1"
                     /translation="MEKYFFDISIFEKIKKVLSYQSKYWEISKPRDKYSFKSQNRISG
                     SNSIKFKDFELDYELESLGKILLKRRSFLLNEFDKNASFDKLIYLLKTVFSPTNCKFV
                     FGEEVPLFSYPTSGALNSITPFVYIDNMDEVKNGIYEVKYDGLEFKKSMNIIDLETFT
                     PITSKNSDVQNNSFKKVNMIIMLCSNYENIFNKYGLLAYRLLHLEAGHIAQNIQLVST
                     ALGIKSLPMGGWFDDKIETFINEISDKKNIYLYAIAVG"
     gene            1199..1363
                     /locus_tag="VQ516_002073"
     CDS             1199..1363
                     /locus_tag="VQ516_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2512447.1"
                     /translation="MNAEDKFRELENENKLLKLHNKELENEIQNGWKFWIWVTVPILG
                     LIYAIIQKIF"
BASE COUNT          563 a          163 c          250 g          553 t
ORIGIN      
        1 ataactatta ttacgaaaaa aaattcttgt gtaaaattct attgactttc tatattattt
       61 ttgtatttca taacgaatgt aatttaacaa gtgaagaaat tttaaagatg gattcctatg
      121 ctattaattt ctgggagagt aaaggggtcg gtattgacaa aaaaatcttg gaatttattc
      181 aaaaaaatat tacagttatt aaaaataaaa attttttgaa taaagatttt gatatttatg
      241 ttaaaaagat tcataattta ttctttgtca tttctacttt agtttcaaaa gagcaataca
      301 tggggatagt ttcttcccaa atacatatgt tcgcaaatag gatgggaatt tctcccacca
      361 ttgaagttac catgctagat ttttttctga gggaggattc tgattaatat ggaaaaatat
      421 ttttttgata tttctatttt tgaaaaaata aaaaaagtat tatcatacca atctaaatat
      481 tgggaaatat cgaagcctag ggataagtac agtttcaaaa gtcaaaatag aatatctggc
      541 tctaatagca ttaaatttaa agattttgag ttagattatg aactggagtc acttggtaaa
      601 atacttttaa aaaggagatc gtttttacta aatgaatttg ataagaatgc gagttttgac
      661 aaactgattt acttgttgaa aactgtattc tctcctacaa attgcaagtt tgtatttgga
      721 gaagaggttc ctttatttag ctatcctaca tcaggtgcgt taaattcaat tactcctttt
      781 gtttatattg ataatatgga tgaagtaaag aatggtatat atgaagtaaa gtatgacgga
      841 cttgaattta aaaaaagtat gaatattata gacttagaaa catttacccc aataacaagt
      901 aaaaacagtg acgtacaaaa taattcattt aaaaaggtaa atatgataat tatgttatgt
      961 tcgaattatg aaaatatttt caataaatat ggattattag cttatcgact tttgcattta
     1021 gaggctggtc atattgcaca aaatatccag cttgtttcta ctgcgctagg aataaaaagt
     1081 ttaccgatgg gaggttggtt tgatgataaa atagaaactt ttattaatga aattagtgat
     1141 aaaaaaaata tttatctcta tgcaattgca gttggataaa tttaaggagg aagtgtagat
     1201 gaacgcagag gataaatttc gcgaactaga aaatgaaaat aaacttctaa aacttcataa
     1261 caaggagctt gagaatgaaa ttcaaaatgg atggaaattt tggatatggg taactgtccc
     1321 tattttaggt ttgatatatg ctattattca aaaaattttt taattatgct agtttgtcag
     1381 gttgagtagt ttttgttcaa aatttaataa acttttttca atgaagatta tctaaagatt
     1441 gcttctcaga tgagaatggc ttctctatct tatataaatt atcttcatag agtgtcctgt
     1501 tattcatgtt atcacagaat agatagttt
//
LOCUS       DAVBCV010000109         1455 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBCV010000109 DAVBCV010000000
VERSION     DAVBCV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.109"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(412..804)
                     /gene="rpsI"
                     /locus_tag="VQ516_002074"
     CDS             complement(412..804)
                     /gene="rpsI"
                     /locus_tag="VQ516_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2512448.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(824..1270)
                     /gene="rplM"
                     /locus_tag="VQ516_002075"
     CDS             complement(824..1270)
                     /gene="rplM"
                     /locus_tag="VQ516_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2512449.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
BASE COUNT          420 a          290 c          279 g          466 t
ORIGIN      
        1 cccaaactct tcacacaaga gttctaactt acccattcta tggaatcttg cattatccat
       61 aatatagtag attgaaacta gaatagtaca cctctacttc taaaacattg ttagaattcg
      121 atttgactat cctcggtttg tcctattctt atttcacctt actataaaaa taaggtaaac
      181 ttttcaaaaa aacacgaaaa aagcacccat agggcaaacc tttgagtgct ttgaaacatt
      241 gatataattg tattgattag cgttttgagg caactgacct tgtcaggttg cagccacatt
      301 tgtaagcaac taaagtcgca acaactgtgt caattgcacc aatttagtaa gaaagtataa
      361 aaaaagaaca ccccgaaagg tgctctgtat aagtatagta attctttcga attaacgttt
      421 actaaattgt gatgctttac gagctttctt aagacctggt ttcttacgtt caactttacg
      481 tgagtcacgt gtaagaagtc ctgcgcgttt caatgaatcg cggaagtctg ggtctacttg
      541 aagaagggca cgagcgatac cgtgacggat agctcctgat tgaccagcgt atccaccacc
      601 tacaacgtta acgaaaacgt cgtatgaacc tacagttgaa gtaactgcga atggttggtt
      661 gatgacaaga cgaaggtcag catgtgggat gtactcttca acatcttttt tgttaacagt
      721 gattttacca gttcctggaa caaggcgaac gcgtgcaaca gcgtttttac gacgtccagt
      781 acctgcatat tgtgcttgtg acatacttta tttgttcctt tccttagata agtcctgaaa
      841 tgtcaagaac ttctggttgt tgtgcagcat gagtgtgctc agctccaaca aatactttca
      901 acttcatacc ttgagcgcgt ccaagtgtat tgtgtggaag cataccttta actgatttct
      961 caatcaaacg tactgcattt ttagaacgaa gttcacctgc agagatttgt ttcaatccac
     1021 ctgggtggtt tgagtgagtg tagtagattt tatcagttgc ttttttacca gtcaatttaa
     1081 ctttttcagc attgataaca atcacgaagt cacctgtatc agtgtgtggt gtaaatgttg
     1141 gtttgttttt tccgcgaagt acgctagcaa ctactgcaga aagacgtcca agtggtacat
     1201 cagttgcgtc aactacgtac catttacgtt caacttggcc tggtttagcc ataaatgttg
     1261 ttttgttcat gatttctcct atatatagtt cgatttttgt ttacggtgat gggtgttcct
     1321 tcccatcgaa aagtatttgg aaggttccgg ggcctttcaa atggggtaaa caataccgcc
     1381 tactatcata ccaaatttca cccctaaaag tcaatagata tgaaaaatat ttttcggtaa
     1441 cttctcaaag taact
//
LOCUS       DAVBCV010000110         1341 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBCV010000110 DAVBCV010000000
VERSION     DAVBCV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.110"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(1..365)
                     /locus_tag="VQ516_002076"
                     /pseudo
     CDS             complement(1..365)
                     /locus_tag="VQ516_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(814..1212)
                     /locus_tag="VQ516_002077"
     CDS             complement(814..1212)
                     /locus_tag="VQ516_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2512450.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          387 a          263 c          212 g          479 t
ORIGIN      
        1 gcttttcctt ttctagggta aggcaaatcc tgcttgctta accccaattt tccatgatga
       61 atccaatagt aaatggttga aattcccacg ttaacccctt tagccataac catcatttca
      121 ggcgaaaatt tttggttatg atagtggaga atcttttcct ttagttcctt ggtcaagctt
      181 gatttcttga ccgagcgctt gcgattgttt tcataagact gttgagcata gtcggcagaa
      241 taaacctctt tgaagcgccc ttttccaaga cattgtcgga ctgtcctacg cttgatttca
      301 gtgtggatag tttgaggagc ttttccaagt agagaggcaa tttctctatt tgatttccct
      361 ttgttcgcct tttgtagtat aatggttttg catctctgtg cctttcttgt gtttgtggtt
      421 gaacaacaag tataacacaa aggtgttttc ttatgcctac aagagctttc attatttcca
      481 ttttcttttg gatttcactc tattctgaaa aacttgtgta tatttactga agctagcaag
      541 tcttacctgt aaatttaatg aaagcaacac aaaatccgag aggggaatct cggattaatg
      601 tcaatttaaa aagatagaga gtttttagtt taaataaatt gttttaaaat atcaacaaca
      661 tcacttcttt tcttaacctg ataagccttg attcctaatt ttggggctac gattatattg
      721 tcctcaatat cgtctagaaa gacacaattt ctaggttata actggtattt atcgatagtt
      781 actcatatat atcagtccac ctccatactc accttacaga ttcttttgat aataatatct
      841 ttgcctaatg tagagatagt cttgcaaaga aaaaacttcc ttgtagccat gtttctgata
      901 aaagtccggt gcctggaact ggtaagtatt gacaaaggca aaacaacaat ttcgattctt
      961 agcttcactt tctgcctgtt gcaatagttt tgaaccgatt ccttgccctc gcagttcctc
     1021 ttttacaaac aaatactcga tttctagcca atttccaaaa gtctctgcta tcaaacccgc
     1081 aaggagattg cccttttcat cttcgacata aagattaagt ggctcacttt cagcctcttc
     1141 tctttttgaa cggttataaa cacgaatcag attccctatt tcttgcgatt tatgtgattc
     1201 cttattttcc aatctaaagt ataatgaaat gaaataaaac atgcgcaaat cgattaagga
     1261 atttaatcta atttctaaca atgtcttaga aatcaaagtg tactatttta acttcaatgc
     1321 actatacatc taatactcaa t
//
LOCUS       DAVBCV010000111         1190 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBCV010000111 DAVBCV010000000
VERSION     DAVBCV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.111"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            239..>1190
                     /locus_tag="VQ516_002078"
     CDS             239..>1190
                     /locus_tag="VQ516_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2512451.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          413 a          210 c          249 g          318 t
ORIGIN      
        1 agccacctta ccttagtcaa gaattagatg tttcactatg tttgagtaag ttgatgattt
       61 catttgataa caggtttgaa actgttaggc taggtggcca aggctaatca tagccttggt
      121 ttagctgaaa aaacaggttc aagggttcct gttgtcaaat gaaatgtgat ttaaggtata
      181 agaaaacacc tctgtgctat acttgttgtt caccacaaac acaaggaaag gcacagagat
      241 gcaagaacat tataccccaa aagggaaaca tttgacaata gataaccgtc gcttgattga
      301 gcggtggaag aatgaaaata agtccaatcg tgaaattgca ggcttgttag gaaaggcgcc
      361 tcaaacgatt cataatgaag tcaaaagagg tacaacttta caacaagtga gaaaagggct
      421 atacaaaaag gtctattctg ccgattacgc acaaactgtt taccaattca atcgaaaacg
      481 gtcggtgaaa aagttaattt taacaaagga aatcagagag aagatcttac actatcataa
      541 gcaaaaattt tcgcctgaaa tgatggttaa caagaagcaa gtgaaagttg gtatttcaac
      601 catctactac tggtttcata atggtcattt aggattgacg aaggccgaca tgctttatcc
      661 cagaaaaagg aaaggtgtca agaagcaagc tagtccgaac tttaagccgg caggtaaatc
      721 tatcgaagaa cgtcctgacg ttattaatct tcgcttggaa aatggtcatt atgaaattga
      781 taccgtccta ctgactaaga taaaaaatta ttgcctgtta gtcttaaccg accggcggag
      841 cagacaccaa attataaggt taattccaaa taaaactgct gaatctgtca atcaggcgct
      901 tacgttacta ttaggggagc atcgtattct gtccattact gcagataatg gttcggagtt
      961 caaacgattg tctgaggtat ttcctgagga acatatctac tacgcacatg cttactcttc
     1021 atgggagaga ggttcaaatg aaaatcataa tcgattaatt cggagatggt tacctaaagg
     1081 aaccaagaaa acgactccga aagaagtagc ttttatcgaa aattggatta acaactaccc
     1141 taaaaaatgc ttggactaca agtcgccaag tgaatttctt ttgggtggct
//
LOCUS       DAVBCV010000112         1054 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBCV010000112 DAVBCV010000000
VERSION     DAVBCV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.112"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..555
                     /locus_tag="VQ516_002079"
     CDS             <1..555
                     /locus_tag="VQ516_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2512452.1"
                     /translation="KDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTE
                     IPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPA
                     THNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPIS
                     NHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(503..1048)
                     /locus_tag="VQ516_002080"
     CDS             complement(503..1048)
                     /locus_tag="VQ516_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2512453.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          380 a          165 c          153 g          356 t
ORIGIN      
        1 aaagacgaaa caacctctta tcctatatat gaaatcccag aagcatggag atatattaaa
       61 tttgctagcc tagttaactt cagaatagga aaaactcctc cacgcagtga agctactttt
      121 tggggaactg aaataccttg ggtatctata tcagatatgc ctatatctgg ttatgtaact
      181 aatacgagag aatctatttc taaattagca ttaaaatcaa agaaaataga catttcacct
      241 aaaggaactt tattaatgag ttttaaatta tctattggaa aagttgccat attggatatt
      301 cctgccactc ataatgaagc cattatatcg atattcccct atgctaataa agaaaatatt
      361 atcagagatt acttaatgat atttttgcca cttatctcta ctttaggtga ttcaaaagat
      421 gctataaaag ggaaaacatt aaatagtacc agtatctccg aattattaat ccctatttct
      481 aatcatgaag agatgaaaag aatcatttcc aaagttgatt tactttttca aaaagtttct
      541 caactttttg agtaataagt tcctgttcct caaaaggagc taacggaatt aatagctcgc
      601 ttagtgtagt tttaggaata ttatataaag cttgacctga tagtttagtt attgctttca
      661 attgtttata aaataacgga gaggacaagt taaataatag aaattttgaa ataatctctg
      721 aactttcgaa tggtgttaat tggaaaataa atccaccagc cacaacacca tcatagtctt
      781 tatcgattct tgcaaacttt ccaatatgtt ctaaagaggt tgatacaggt gttattagct
      841 gattatgttt taaataaact tgctcagagg agatgaattg tgtatcaatg tagtaatcat
      901 tatccaacag agaaaattct aaaggcttaa tattaccacc acgtataatt ctaacacctt
      961 tattattaat gcttaaatcg cccttcttgt aagaaagacc tgtatttatt gaaaaaatat
     1021 cttttatttt tataacaacc caattcatag gtat
//
LOCUS       DAVBCV010000113         1035 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBCV010000113 DAVBCV010000000
VERSION     DAVBCV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.113"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ516_002081"
     CDS             complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ516_002081"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2512454.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSL"
BASE COUNT          270 a          226 c          183 g          356 t
ORIGIN      
        1 caatgagaaa tagtttatcc atcctcgaat agacaaattc agttgctcaa tacgtcttgt
       61 taggtctata ctccatttcc tctgtgttag tttcttcaat ttaagcttaa atctccaaac
      121 actatcttga tgtggacgac ttttccaacc atctgatgat ttccagaacc cgaaacctag
      181 atatttcaac tctcttggtc tggtaatctt agtcttggtc atgtttactt tcagacctag
      241 ccgtttctca ataaaacgac tgactgaata catcacacgc ttagcggctg cctcgcttcc
      301 gaccgtaatc acacaatcgt ctgcgtagcg aacaaatcga agccctctct tttctaattc
      361 cttgtccaat tcattaagca tgatattgga taagagagga gataaatttc ctccctgtgg
      421 cgtgccaact agcgttttat aacgttgacc gttaatgata acacctgaat gaagatattt
      481 acgaatcaag gattccgtat ctccgtcttc gataatgtta tgtactaagg acatcaatct
      541 atcttgagga actgtatcga aaaatttctc taggtctatg tccactatcc actcatagcc
      601 gtcatttaag tactctaaga gcttcatgat ggctttttca catgacctat ttggtctgaa
      661 tccatagctc gtatctgaga aatggggttc acaaataggg ctaataactt gaacaatggc
      721 ctgttgaatc attctatcca taactgttgg aattcctagt tggcggatgc ctccgtttgg
      781 tttgggaatc tcaactctaa gaactggttg aggcttatat tttctctgtt ttatcagttc
      841 cttagtcagg cgccagtttt gtctgagata gccatccatc tcttcgatag taattccatc
      901 aatcccagct gagcctttat tagattttac ttgattgtag gcttcaagca tattgtcgcg
      961 tgataatatt ttatctagca attttgacat gtgcctattc ctttcttgtt ttggtgcctg
     1021 agggacatct tatat
//
LOCUS       DAVBCV010000114          902 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBCV010000114 DAVBCV010000000
VERSION     DAVBCV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.114"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            122..590
                     /locus_tag="VQ516_002082"
                     /pseudo
     CDS             122..590
                     /locus_tag="VQ516_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          331 a          135 c          166 g          270 t
ORIGIN      
        1 gagaagagga ttgccatctt ctccattttt atgtgcagag ttatagtggc ttgatgctgg
       61 gaaagtacac tgtgactgct aaaacattcc tagaagctgc tttgatttcc ctaatctatt
      121 tatgcaaatt ttatgttatt ttactataac agttgttgct aagccaaata atagtgggga
      181 agttcattta gacgtaagca ttgaagataa tcagggaggt agtgggtata atttcagttc
      241 tgtttcaagt agctcacaaa cagctaaata tgaaggaact ggttataata acaattcatc
      301 attatatata acgattgata aaacgtctga tgcaacagct cttttgaaat taaagttgaa
      361 taatgttgat aatcaacctg ctactgaagt tcctagttca ggaattactg taaaattaaa
      421 tgctaaagat aatgctggaa actggacaag tgcttcgaat aaagacaagt gcttcgaata
      481 aaaaagaaat aacagtaaaa attgtttctg ctaaaccgac atatccagac aaaatcttag
      541 tgaaaaatcc tgataatata aaagatacag aaaaaaatgc cattattgaa aaattgaaag
      601 aggcaaataa aaatcatcca gcaggagctc caacctttgc taaaggtgaa ggagagcatg
      661 caaatgatat tgtagcaact tattcagatg gtacaactta ttatgtaccg ttaaatgatg
      721 tgacaaaata tgcgaggtag tggctgtacc actcacttat tcacctcccc gtgatttgta
      781 gtagtgatag gctttctcac tattattata aaacaaaata aagatcacaa cactttttca
      841 ttctgtgttg tgccttgagt gaaacgaaag gaatgaatta taaatatgaa aagtatagtc
      901 ac
//
LOCUS       DAVBCV010000115          890 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBCV010000115 DAVBCV010000000
VERSION     DAVBCV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.115"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(1..229)
                     /locus_tag="VQ516_002083"
                     /pseudo
     CDS             complement(1..229)
                     /locus_tag="VQ516_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            498..890
                     /locus_tag="VQ516_002084"
                     /pseudo
     CDS             498..890
                     /locus_tag="VQ516_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          293 a          169 c          169 g          259 t
ORIGIN      
        1 cagtattaca tccaccagct attaagaaaa atgattcgcc tgtatgggca tcaacagctc
       61 cataacaata gtgaaattct cgtatatagt gactatggac atgtggacct actcctattg
      121 gagaccaaca agatcccagt ttactgattc taccgaagcc agcctcatct tggtacatca
      181 agcgaacctt atgaaaacgt ctactggttt taaagcgctt tcttttcttc agttgatttc
      241 atatcactat tgtataatgc tttttgattt tagtctagta taaatagcga gaaatatcta
      301 tcctatcttc tagaatgtct tccaaacgag gaaactctcg taaacaaaga ggttttagag
      361 gcctatttac cgtggactaa agttgcacaa gaaaagtgca aataagaaat ctccagatta
      421 ggaactatcc gtgagttctc tagtctggag atttttcaat agacttcctg cgaaacaaaa
      481 tatggtataa tagttctatg aattatgaag caagtaaaca actaaccgat gcacgattta
      541 agcgtcttgt tggtgttcaa cgcacgactt ttgaagagat gttagctgta ttaaaaacag
      601 cttatcaact taaacacgca aaaggtggac gaaaacctaa attaagccta gaagaccttc
      661 ttatggccac tcttcaatat gtgcgagaat atcgaactta tgaagaaatt gcggctgatc
      721 ttggtattca cgaaagcaac ttactccgtc ggagccaatg ggttgaagta actcttgttc
      781 aaaggtggtg ttgcgatttc aagaactcct ctcagttctg aggacacggt aatgattgat
      841 gcgacggaag taaaaatcaa tcgacctaaa aaaagaatta gcgaattatt
//
LOCUS       DAVBCV010000116          860 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBCV010000116 DAVBCV010000000
VERSION     DAVBCV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.116"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            751..>860
                     /locus_tag="VQ516_002085"
     CDS             751..>860
                     /locus_tag="VQ516_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HET2512455.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIV"
BASE COUNT          296 a          133 c          158 g          273 t
ORIGIN      
        1 gcgccattaa atcattggtt atgggacgga gtaaaagagt ccagtggact cttttagcct
       61 gagctcagtt taaaaaagcg agggtggtta ttttctcaaa gttttgaagg agctaaagca
      121 agagctatta ttatgagctt gttggaaaca gctaaacgtc atcaattata gtgcgttgaa
      181 tctataacag tacgcatcga ctgctaaaac atttctataa atcaattttc ctttcctaat
      241 cgatttgttc atatcttatt tcaatccatt ataaatagcg agaaatatct atcctatctt
      301 ctagaatgtc ttccaaacga ggaaactctc gtaaacaaag aggttttaga ggcctattta
      361 ccatggacta aagttgtaca agaaaagtgc aaataagaaa tctccagatt aggaactatc
      421 cgtgagttca ctaatctgga gatttttcaa tagacttcgt tattgggcgg ttacgatatg
      481 atcactgact tcgtcagtct tatctacaac ctcaaaactg tgttttgagc aacctgcggc
      541 tagcttccta gtttgctctt tgattttcat tgagtattag aacagaaaaa atacttggag
      601 tatttgtttg tgtgtttatt tttatataac aaactataaa caaaacaaaa atataaaaaa
      661 agagacaaaa aagaacagaa agcaattgac aacggtttca tatagtgtta tacttatggc
      721 ataaagttaa tggaaagaag ggaaaaactt atggtattag attatttctt tgacaaaaac
      781 cttgtgtttt gcttagaagc ggataatcaa gaacaactct ttgatcaggt tgcaacttta
      841 ttggaagaac gagaaattgt
//
LOCUS       DAVBCV010000117          849 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBCV010000117 DAVBCV010000000
VERSION     DAVBCV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.117"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(173..250)
                     /locus_tag="VQ516_002086"
                     /pseudo
     CDS             complement(173..250)
                     /locus_tag="VQ516_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            408..626
                     /locus_tag="VQ516_002087"
     CDS             408..626
                     /locus_tag="VQ516_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET2512456.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          266 a          127 c          163 g          293 t
ORIGIN      
        1 gtatgccgaa cggcacgtac ggtggtgtga gaggggctag agattatccc ctactcgatt
       61 tcgaaatcta gtggaatgaa tctggaatag tccatcgagc tttctaatac tcttcgaaaa
      121 tctcttcaaa ccacgtcaac gtcgccttgc cgtgcgtatg gttactgact tcgtcagttc
      181 tatccacaac ctcaaaacag tgttttgagc aacctgaggc tagtttccta gtttgctctt
      241 tggttttcat ttagtataac agattgttag aagttggttt aaatttccta atcagtttgt
      301 tcacatttac cttcgatata ttatatccca tagttaaggt tggtcataca gatgattata
      361 gtcatggagc cgtaaaactt agtgtttctt tagttgacaa agatgccatg aaaaaaatat
      421 ctgtaactgt aataggatat tttgaaataa atatagatga aaatatcacc gatattctat
      481 acgtaaatgg tactgctatt ctttatcatt atttacgttc tattgtttca atagtttcgg
      541 caattgatag cagtgaagca atgttgctac ctaccattaa tgttttagag ttactagata
      601 aatctcaacc ttttgaagaa gaataattta ttagctcact aaattgaggg taaggaaaag
      661 taaaatcagt aagaaaaatg tcttgcatta tacagcaacc ttttgggaat gagtagatgg
      721 attgaataaa attttattaa gagtggatga tttatctgta gattattatt ggacagttag
      781 tcttgaagta gtctaagaat taggttataa tcagtagaat ccttgctaat aatgaggagg
      841 ttagtttat
//
LOCUS       DAVBCV010000118          832 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBCV010000118 DAVBCV010000000
VERSION     DAVBCV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.118"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            129..410
                     /locus_tag="VQ516_002088"
                     /pseudo
     CDS             129..410
                     /locus_tag="VQ516_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(544..631)
                     /locus_tag="VQ516_002089"
     tRNA            complement(544..631)
                     /locus_tag="VQ516_002089"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(595..597),aa:Ser,seq:gct)
     gene            complement(638..711)
                     /locus_tag="VQ516_002090"
     tRNA            complement(638..711)
                     /locus_tag="VQ516_002090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Ile,seq:gat)
     gene            complement(747..817)
                     /locus_tag="VQ516_002091"
     tRNA            complement(747..817)
                     /locus_tag="VQ516_002091"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(783..785),aa:Gly,seq:tcc)
BASE COUNT          288 a          184 c          139 g          221 t
ORIGIN      
        1 tactagacat taaagaccca aacatcaaga ttctagatat catcaatatg gatacccaca
       61 aagaaattat cgctaagctg gattatgagg ctccatcttg ccctgattgt ggaagtctaa
      121 tgaagaaaaa ggaaccagag aaatttttcg gacttattga ggacaatctt aagcaggttc
      181 atcctatttt tcagactgtc tttaaaacct tcctcaaaga taaagaaaag attgtcaacg
      241 cccttcaact acactattct aacgccaaac tggaagcgac caataatctc atcaaactta
      301 tcaagtgcaa tgcctttggt tttcgaaact ttgaaaactt taaaaaacgg atttttatcg
      361 ctttgaacat caaaaaagaa aggacgaaat ttgtcctttc tcgagcttag cttttcttca
      421 acccactaca gttgacaaag agccataaaa ataacttcag attagtaatt caattaagtt
      481 ttacggatct gaagctttat tgtctctaaa aataaaaaag agagttacaa actctcatta
      541 aaacggagaa taagggattc gaacccttgc gccagttacc cgacctaacg atttagcaaa
      601 ccgtcctctt cagcctcttg agtaattctc caattaatgg gcacgagtgg actcgaacca
      661 ccgacctcac gcttatcagg cgtgcgctct aaccacctga gctacgcgcc caagttaaaa
      721 aacttggtaa tttgaacaaa gttcaaagcg ggtgacgaga atcgaactcg cgacaacagc
      781 ttggaaggct gtagttttac cactaaacta cacccgcata aatactatca at
//
LOCUS       DAVBCV010000119          830 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBCV010000119 DAVBCV010000000
VERSION     DAVBCV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.119"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            21..830
                     /locus_tag="VQ516_002092"
                     /pseudo
     CDS             21..830
                     /locus_tag="VQ516_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
BASE COUNT          266 a          174 c          162 g          228 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt gaccgaaagt ccgattcagc gtccaaaaaa aaccaaagca aaaattattc
      421 tggtaaaaag aaacgacaca ccttaaaaac tcaaattatg ctggatttga cgacacataa
      481 agtctgtcaa atggcctttt ctgacggaca tacgcatgat tttactctct tcaaagaaag
      541 tattggacaa agtttgcctg aaacgacgct tgcctttgtt gacctaggtt atttaggcat
      601 cttgaaattt catgagaata ctttcattcc tgctaaaaat tccaaaaatc gccgcctgag
      661 tgaggatgat aagcagttaa ataaagagat gtcagcgata cgaattgaaa ttgaacattt
      721 taacgctaaa ttcaagacct tccaaatcat gtcagtccct tatcgtaacc gcagaaaacg
      781 tttcgagtta cgggcggaat taatttgtgc catcatcaat tatgaagtga
//
LOCUS       DAVBCV010000120          771 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBCV010000120 DAVBCV010000000
VERSION     DAVBCV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.120"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..>771
                     /locus_tag="VQ516_002093"
     CDS             <1..>771
                     /locus_tag="VQ516_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HET2512457.1"
                     /translation="RIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDF
                     SRLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQV
                     KIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYK
                     AIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLF
                     HFQNKDPEKFFGLIEDNLKQVHPLFQTVFKTFLKNKEK"
BASE COUNT          241 a          158 c          125 g          247 t
ORIGIN      
        1 tcgtatcatc aaccaaaaaa ttgctcaaaa gttaattgaa aaaatttcta tgactgatat
       61 tgcccatcag ctggccattt caacttcaac tgtcattcgc aagctcaatg actttcactt
      121 tgagcatgat ttttctcggc ttccaaagat tatgtcttgg gatgagtatg ccttcactaa
      181 gggaaagatg agtttcattg cgcaagattt tgataatctt aatattatca ctgttcttga
      241 aggcagaaca caagctgtca tccgaaatca ctttcttcgc tacgatagag ccgttcgttg
      301 tcaagtgaaa atcattacga tggatatgtt tagtccttac tatgacttgg ctaaacagct
      361 ttttccgtgt gctaaaatcg ttctagatcg tttccatatt atccaacatc tcagccgtgc
      421 catgagtcgt tttcgtgttc aaattatgaa tcagtttgaa cgaaaatctc atgaatacaa
      481 ggctatcaag cgttactgga aactcatcca acaggatagt cgtaaattga gcgataaacg
      541 tttttatcgc cctacttttc gcatgcactt aacaaataaa gaaattcttg acaagatttt
      601 aagctattca gaagacttga aacaccacta tcagatctat caactcttac tttttcactt
      661 tcagaacaaa gaccctgaga aatttttcgg actcattgag gacaatctga agcaggttca
      721 tcctcttttt cagactgtct ttaaaacctt tctaaagaac aaagagaaaa t
//
LOCUS       DAVBCV010000121          692 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBCV010000121 DAVBCV010000000
VERSION     DAVBCV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.121"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(74..147)
                     /locus_tag="VQ516_002094"
     tRNA            complement(74..147)
                     /locus_tag="VQ516_002094"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VQ516_002095"
     tRNA            complement(157..242)
                     /locus_tag="VQ516_002095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VQ516_002096"
     tRNA            complement(250..321)
                     /locus_tag="VQ516_002096"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VQ516_002097"
     tRNA            complement(344..416)
                     /locus_tag="VQ516_002097"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="VQ516_002098"
     tRNA            complement(435..516)
                     /locus_tag="VQ516_002098"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="VQ516_002099"
     tRNA            complement(522..594)
                     /locus_tag="VQ516_002099"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="VQ516_002100"
     tRNA            complement(619..691)
                     /locus_tag="VQ516_002100"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
BASE COUNT          179 a          205 c          149 g          159 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt accttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ct
//
LOCUS       DAVBCV010000122          690 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBCV010000122 DAVBCV010000000
VERSION     DAVBCV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.122"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            1..686
                     /locus_tag="VQ516_002101"
                     /pseudo
     CDS             1..686
                     /locus_tag="VQ516_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          213 a          151 c          113 g          213 t
ORIGIN      
        1 attactatgg atatgtttag cccttactat gacttagcta gacagcttcg ctttcaaatt
       61 tctaggctca ggctgaaaca gtctcccaga ctgtttcact cccgaatgct aaaatcgttc
      121 ttgatcgctt tcacattgtg caacatctta gccgtgctat gagtcgtgtg cgtgtttaaa
      181 tcatgaatca gtttcatcga aaatcccatg aatacaaggc tatcaagcgc tactggaagc
      241 tcatacaaca ggatagccgt aaactgagtg ataagcgttt ttatcgtcct acttttcgta
      301 tgcatttaac caataaagag attttagaca agcttttgag ctattcggaa gacttgaaac
      361 accactatca gctctatcag ctcttgcttt ttcacttcca gaataaggaa ccagagaaat
      421 ttttcggact tattgaggac aatctgaagc aggttcatcc tatttttcag actgtcttta
      481 aaaccttcct caaagataaa gaaaagatta tcaacgccct tcaactaccc tattcaaacg
      541 ccaaattgga agcgaccaat aatctcatca aacttatcaa gcacaatgcc tttggttttc
      601 ggaactttga aaacttcaaa aaacggattt ttatcgctct gaatatcaaa aaagaaagga
      661 cgaaatttgt cctttctcga tcttagcttt
//
LOCUS       DAVBCV010000123          680 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBCV010000123 DAVBCV010000000
VERSION     DAVBCV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.123"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            1..680
                     /locus_tag="VQ516_002102"
                     /pseudo
     CDS             1..680
                     /locus_tag="VQ516_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          205 a          138 c          124 g          213 t
ORIGIN      
        1 ttcaagtgct atcactgttc aaaaatgatg gtcgctgaaa cttctatcgt caagaataat
       61 caccaaatcc ctcgtatcat caaccaaaag attgctcaaa agttaattga aaagatttct
      121 atgactgata ttgcccatca gctttccatc tcaacttcaa ctgttattcg taagctcaat
      181 gacttccact ttgagtgtaa ttttagaaat ctgcctgaga ttatgtcttg ggacgttgaa
      241 acagtccggg gagtgactgt ttcaatcggg agatggagat gagcttcatt gcgcaagatt
      301 ttaacaatct caatatcatc actgttcttg agggtagaac acaagctgtc atccgagatc
      361 actttcttaa atatgataga gccgtccgat gtcgcgtcaa aattattact atggatatgt
      421 ttagtcctta ctatgactta gctagacaac ttttcccatg tgctaaaatc gtgttggatc
      481 gctttcacat tgtacaacat cttagccgtg ctatgagtcg tgtgcgtgtt caaatcatga
      541 atcaattgga tcgaaagtct catgaataca aggctatcaa gcgctactgg aagctcatac
      601 aacaggatag tcgtaaactc agcgataaac atttttatcg ccctactttt cgtatgcatt
      661 taaccaataa agagatttta
//
LOCUS       DAVBCV010000124          631 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBCV010000124 DAVBCV010000000
VERSION     DAVBCV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.124"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
BASE COUNT          196 a          121 c          161 g          153 t
ORIGIN      
        1 taggctgatt ttaagtacga cgggcatgtc gtgcatctga ggtgtaagtc ctccgtgggc
       61 acctgctacc ggtgaaccca atagcgattc ccaagcctga ctatcgtgag gtagcggatt
      121 aaaacggtct ggggatagac cgttttaagt ctgacgcagg aaataagaat cgtcagagga
      181 agggatagcg aaatcgaggc tctacgaaca ggaacgtgat agtaaggcgt atacagcgga
      241 taagggggct taaaactcta aagtccaaaa aggtagtcgt aacctatatg cgtaaatcac
      301 gagagtaatt gaattcggac taaggtctat gtgaaaaaga taaatcttcc tagagtctaa
      361 agactctgcg tcagatttcc tattttcatt gtaacctttt aacgtcctca tatcttgtat
      421 aaacgaggaa agatgtacgg cttatcccgt gaggtctcat gagcgccgaa agcgtagtaa
      481 caacgaatca tgagaagtca gccgagccca tagtagtgag gaaacttctg taatggaagt
      541 ggagcgaagg ggtaaacaac taatcgaagt aatcctactt cacttgtgtc tgtaaaacga
      601 acggtctgat agaactggac tctgtcacgt a
//
LOCUS       DAVBCV010000125          611 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBCV010000125 DAVBCV010000000
VERSION     DAVBCV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.125"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(189..>611)
                     /locus_tag="VQ516_002103"
     CDS             complement(189..>611)
                     /locus_tag="VQ516_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2512458.1"
                     /translation="KPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          155 a          121 c           93 g          242 t
ORIGIN      
        1 gatgaagttt tttctcaact tcttttactt tattaaatag ttttctaaac cggttattta
       61 accatttaat taaccagtaa atagtttact atttaaaatt acttttgtca agtttctgtt
      121 acgtttttct aaaaaagaga agtaacacga aaggttactt ctccataaaa tatgctccat
      181 tcattttact actgtatagg agtcggttga ctttctttta acaaagccaa gagactatct
      241 acttctgctg aaatagtgtt attatctttc gttccgagaa gaagactact ttttagacca
      301 gtcaatgtct ccacagcatt ttgtctaata ctagaatctg ttactttttc tagcaaggcc
      361 tccgcctctg ctatcttagc gttaataaca gaatgctcta cttgaggaat ttcagcctca
      421 tctgtagtat cttcagcttc ttcctctgtc tcttccgtat cagtgcttgg tttataaaga
      481 ttatctgctg aaggatttaa accagcgtga ttctcttttt catcagattc agggtgagtt
      541 ggctcactta cttcatcatg ttccttatct tcctcaggtt tttctgtctg aggtttctcc
      601 tcgtttggtt t
//
LOCUS       DAVBCV010000126          560 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBCV010000126 DAVBCV010000000
VERSION     DAVBCV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.126"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..>560
                     /locus_tag="VQ516_002104"
     CDS             <1..>560
                     /locus_tag="VQ516_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2512459.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPL
                     DRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYV
                     EHPNERPHSDNGFGNASDHVQRNKNG"
BASE COUNT          183 a          114 c          126 g          137 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatca ggattcagga
      241 aatactgagg caaaaggagc agaagctatc tacaaccgcg tgaaagcagc taagaaggtg
      301 ccacttgatc gtatgcctta caatcttcaa tatactgtag aagtcaaaaa cggtagttta
      361 atcatacctc attatgacca ttaccataac atcaaatttg agtggtttga cgaaggcctt
      421 tatgaggcac ctaaggggta tactcttgag gatcttttgg cgactgtcaa gtactatgtc
      481 gaacatccaa acgaacgtcc gcattcagat aatggttttg gtaacgctag cgaccatgtt
      541 caaagaaaca aaaatggtca
//
LOCUS       DAVBCV010000127          534 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBCV010000127 DAVBCV010000000
VERSION     DAVBCV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.127"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
BASE COUNT          172 a           89 c          139 g          134 t
ORIGIN      
        1 ctgactatcg tgaggtagca gggagaggaa gggatagcga aatcgtggct ctacgaacag
       61 gaacgtgata gtaaggcgta tatagcggat aaggaggctt caaactctaa agtccaaaaa
      121 ggtagtcgta acctatatgt gtaaatcacg agagtaattg aattcggact aaggtttgtg
      181 tgaaaaagat aaatctttct agagtctaaa gactctgcgt cagatttcct attttcactg
      241 taacctttta acgtcctcat atcttgtata aacgaggaaa gatgtacgac ttatcccgtg
      301 aggtttcatg agcgctgaaa gcgtagtaac aacgaatcat gagaagtcag ccgagcccat
      361 agtagtgagg aaacttccgt aatggaagtg gagcgaaggg gtgaatactc aaacagtctg
      421 gggagagact gtttgaggtc tgtcgctaga aagagaaaac gacagatcga agtaatccta
      481 cttcacttgt gtctgtaaaa tgagtggtct gatagaactg gactttgaca cgta
//
LOCUS       DAVBCV010000128          494 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBCV010000128 DAVBCV010000000
VERSION     DAVBCV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.128"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(53..126)
                     /locus_tag="VQ516_002105"
     tRNA            complement(53..126)
                     /locus_tag="VQ516_002105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Met,seq:cat)
     gene            complement(140..229)
                     /locus_tag="VQ516_002106"
     tRNA            complement(140..229)
                     /locus_tag="VQ516_002106"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Ser,seq:tga)
     gene            complement(312..385)
                     /locus_tag="VQ516_002107"
     tRNA            complement(312..385)
                     /locus_tag="VQ516_002107"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(349..351),aa:Ile,seq:gat)
     gene            complement(420..490)
                     /locus_tag="VQ516_002108"
     tRNA            complement(420..490)
                     /locus_tag="VQ516_002108"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Gly,seq:tcc)
BASE COUNT          141 a          137 c          109 g          107 t
ORIGIN      
        1 cgacacatgg agcttcaatc cattgctcta ccaactgagc taccgagcct tattgcggga
       61 gcaggatttg aacctacgac cttcgggtta tgagcccgac gagctaccga gctgctccat
      121 cccgcgttaa taatataaaa ggaggatgtg ggattcgaac ccacgcacgc ttttacacgc
      181 ctgacggttt tcaagaccgt tcccttcagc cggacttggg taatcctcca aaatagttta
      241 tacgggaaca agcccgtgtc ctcttagtga acagataata ttccaatccc ctagtaatgg
      301 actaaacact atgggcacga gtggactcga accaccgacc tcacgcttat caggcgtgcg
      361 ctctaaccac ctgagctacg cgcccaagtc aaaaacttgg tacaaagaac aaagttcaaa
      421 gcgggtgacg agaatcgaac tcgcgacaac agcttggaag gctgtagttt taccactaaa
      481 ctacacccgc taaa
//
LOCUS       DAVBCV010000129          416 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBCV010000129 DAVBCV010000000
VERSION     DAVBCV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.129"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            134..>416
                     /locus_tag="VQ516_002109"
     CDS             134..>416
                     /locus_tag="VQ516_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512460.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSL"
BASE COUNT          131 a           89 c           66 g          130 t
ORIGIN      
        1 tctaaccaca gcttttgttt caattggtaa tgagtttgat aacgcataga cgtttgatct
       61 cgtctaaggt aacaaggcaa aaaattcgtg gataaccaag tagacttgaa atactatatc
      121 tggaggtact cccatgcttt atgtgggcat tgatatcgct aaaaataaac acgatgttac
      181 agccttgaat gttccaggaa aaactgttct taaaccactc actttttcaa ataataaagc
      241 tggttttgaa ctcttagatc tgtctcttcg acagctcaac caagactgtc tcatcgctct
      301 tgaaaatacc ggacattatg cctttaatct actgaacttc ctgcatgaac aaggatataa
      361 agtttacacc tacactcctt tgctcatcaa ggaatttgcc aaatcattat cacttc
//
LOCUS       DAVBCV010000130          393 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBCV010000130 DAVBCV010000000
VERSION     DAVBCV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.130"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..>393
                     /locus_tag="VQ516_002110"
     CDS             <1..>393
                     /locus_tag="VQ516_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2512461.1"
                     /translation="YLHLWELSDWDQEGMLCLHELISREEGLVDDIPRLRKYFKTKFR
                     NRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLWLDDYYLFHETLRDYRNKQSK
                     EKQEELERVLSNERFRGRQRVLRDLRIVF"
BASE COUNT          143 a           57 c           93 g          100 t
ORIGIN      
        1 taccttcatt tatgggaatt gtcggattgg gaccaagaag gcatgctctg cttacatgaa
       61 ttgattagta gagaagaagg actggtagac gatattccac gtttaaggaa atatttcaaa
      121 accaagtttc gaaatcgaat tttagactat atccgtaagc aggaaagtca gaagcgtaga
      181 tacgataaag aaccctatga agaagtggga gagatcagtc atcgtataag tgaggggggt
      241 ctctggctag atgattatta tctctttcat gaaacactaa gagattatag aaacaaacaa
      301 agtaaagaga aacaagaaga actagaacgc gtcttaagca atgaacgatt tcgagggcgt
      361 caaagagtat taagagactt acgcattgtg ttt
//
LOCUS       DAVBCV010000131          340 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBCV010000131 DAVBCV010000000
VERSION     DAVBCV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.131"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
BASE COUNT          128 a           41 c           62 g          109 t
ORIGIN      
        1 tgttttgaac aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtattag
       61 aacagaaaaa atgcttggag tatttgtttg tgtgtttact tttatataac aaactataaa
      121 cacaacaaaa atataaaaaa agagacaaaa aagaacagaa agtaattaac aacgatttca
      181 tatagtgtta tacttatagc ataaagttaa tggaaagaag ggaaaacctt atggtattag
      241 attatttctt tgacaaaaac cttgtgtttt gcttagaagc ggataatcaa gaataactct
      301 ttgatcaggt agcaagcttg ttggaggaac gagaaattgt
//
LOCUS       DAVBCV010000132          326 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBCV010000132 DAVBCV010000000
VERSION     DAVBCV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.132"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            135..326
                     /locus_tag="VQ516_002111"
                     /pseudo
     CDS             135..326
                     /locus_tag="VQ516_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          100 a           65 c           57 g          104 t
ORIGIN      
        1 tctaaccaca gctttttgtt tcaattggta atgagtttga taacgcatag acgttttatc
       61 tcgtctaagg taacaaggca aaaagttcgt ggataaccaa gtagacttga aatactatat
      121 ctggaggtac tcccatgctt tatgtgggca ttgatgtcgc taaaaataaa cacgatgtta
      181 cagccttgaa tgttcgagga aaactgttct taaaccactc actttttcaa ataataaagc
      241 tggttttgaa ctcttagatc tgtctcttcg acaactcaac caagactgtc tcatcgctct
      301 tgaaaatacc ggacattatg ccttta
//
LOCUS       DAVBCV010000133          308 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBCV010000133 DAVBCV010000000
VERSION     DAVBCV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.133"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            77..>308
                     /locus_tag="VQ516_002112"
     CDS             77..>308
                     /locus_tag="VQ516_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET2512462.1"
                     /translation="MIKILAACGAGVNSSYQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPIL"
BASE COUNT          102 a           57 c           61 g           88 t
ORIGIN      
        1 gaaaaacttt atgcttacat tgctgaagca accgcttaat cttgtctatt aaaaaataaa
       61 atcggaggaa atccaaatga ttaaaattct tgctgcctgc ggtgcaggtg ttaactcaag
      121 ttaccaaatt aaaagtgctc tagaagaaga actttcaaac cgtggttatg atgttcactg
      181 tgatgcagtc atggtgaaag atgtaaacga agaccttatg aaaggttacg atatctttac
      241 accaatcgct gcaacagacc ttggttttga accaggtatc ccagttatcg aagctgggcc
      301 aatcttat
//
LOCUS       DAVBCV010000134          300 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBCV010000134 DAVBCV010000000
VERSION     DAVBCV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.134"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(1..199)
                     /locus_tag="VQ516_002113"
                     /pseudo
     CDS             complement(1..199)
                     /locus_tag="VQ516_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           91 a           42 c           59 g          108 t
ORIGIN      
        1 ttagaatagt gtagttgaag ggcattgaca atcttctctt tatctttgag gaaggtttta
       61 gaggatgaac ctgattcaga ttgtcctcaa tgagtccgaa aaatttgtca ggctccttat
      121 tctgaaagtg aaaaagcaag agttgataga gattatagtg gtgtttcaag tcttctgaat
      181 agctcaaaag tttatctata gtagattgaa actagaatag tacacctctg cttctaaaac
      241 attgttagaa atcgatttga ctgtcctgaa tgatttgtcc tgttattatt tcattttact
//
LOCUS       DAVBCV010000135          292 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBCV010000135 DAVBCV010000000
VERSION     DAVBCV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.135"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            51..>292
                     /locus_tag="VQ516_002114"
     rRNA            51..>292
                     /locus_tag="VQ516_002114"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           90 a           54 c           81 g           67 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga
       61 gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct
      121 gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct
      181 gcctggtagc gggggataac tattggaaac gatagctaat accgcataag agtagatgtt
      241 gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg cg
//
LOCUS       DAVBCV010000136          288 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBCV010000136 DAVBCV010000000
VERSION     DAVBCV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.136"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(163..236)
                     /locus_tag="VQ516_002115"
     tRNA            complement(163..236)
                     /locus_tag="VQ516_002115"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(200..202),aa:Asn,seq:gtt)
BASE COUNT           99 a           66 c           42 g           81 t
ORIGIN      
        1 ctgttcgctc acaataagag agaacttttt tacgaaaatc tattgaatat gccataaaaa
       61 gattatacca cattgtgtac tatttttggt tcattttact ataacacgaa atagattatt
      121 attacataac aaaaaagagg tctaaacctc ttaactaaat tactccgcca gtaggactcg
      181 aacctacgac atcatgatta acagtcatgc gctactacca actgagctat ggcggattaa
      241 agctaagcga cttccctatc tcacaggggg caacccccaa ctacttcc
//
LOCUS       DAVBCV010000137          277 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBCV010000137 DAVBCV010000000
VERSION     DAVBCV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.137"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            <1..>277
                     /locus_tag="VQ516_002116"
     CDS             <1..>277
                     /locus_tag="VQ516_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2512463.1"
                     /translation="QRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAM
                     QGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQG"
BASE COUNT          126 a           37 c           47 g           67 t
ORIGIN      
        1 aacaacgcat aatagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
       61 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      121 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      181 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      241 agaaagattt ggacatttct attgtttccc aaggaga
//
LOCUS       DAVBCV010000138          270 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBCV010000138 DAVBCV010000000
VERSION     DAVBCV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.138"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..>270)
                     /locus_tag="VQ516_002117"
     CDS             complement(<1..>270)
                     /locus_tag="VQ516_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512464.1"
                     /translation="RKEISNWHIKASQYYLESLYKLLREKLLEQPLLHADETSYRVLE
                     SDSHLTYYWTFLSGKAENQAITLYHHDQRRSGLVVQEFLGDYSGYV"
BASE COUNT           82 a           58 c           51 g           79 t
ORIGIN      
        1 acatagccag aataatctcc taggaattct tgtactacta aaccactccg acgctgatca
       61 tggtggtaca gcgtgattgc ttgattctca gctttcccag acaaaaaagt ccaatagtag
      121 gtcagatggc tatcactctc tagaacccga taagaggttt catccgcatg aagaagaggt
      181 tgttctaaca acttttctcg taaaagctta taaagggact ctaaatagta ttgacttgcc
      241 ttgatatgcc aattagagat ttccttacgt
//
LOCUS       DAVBCV010000139          261 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVBCV010000139 DAVBCV010000000
VERSION     DAVBCV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.139"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
BASE COUNT           83 a           45 c           51 g           82 t
ORIGIN      
        1 atagagctcg ctcttactat tatagcggat gttatagctg gaattatctt gtatttcgtc
       61 tgcagatggc tagatagtaa ggagtagacc gagtgactag cctataaaca cccgttaaat
      121 cgctaagata cgtcaaaaaa gcccttaact acggccatag ttaggggctt tggtgttcta
      181 atgaacctta tcaattaact acattttagc atataatcta caatagttca agcattaagt
      241 gaggttgatg atctaaattt a
//
LOCUS       DAVBCV010000140          229 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain TNE6086
            SAMEA1919802-rid20174453.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVBCV010000140 DAVBCV010000000
VERSION     DAVBCV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919802
            Sequence Read Archive: ERR298088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980253.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980253.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,079
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 68 of 141
            Pseudo Genes (incomplete)         :: 68 of 141
            Pseudo Genes (internal stop)      :: 37 of 141
            Pseudo Genes (multiple problems)  :: 28 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919802-rid20174453.denovo.140"
                     /strain="TNE6086"
                     /db_xref="taxon:1313"
     gene            complement(<1..>229)
                     /locus_tag="VQ516_002118"
     CDS             complement(<1..>229)
                     /locus_tag="VQ516_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2512465.1"
                     /translation="FLKPLYNLLRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSG
                     KVENQAITLYHHDQSRSGSVVQEFLGDYSGYV"
BASE COUNT           70 a           50 c           49 g           60 t
ORIGIN      
        1 acatagccag aataatctcc taggaattct tgtactaccg aaccactccg actctgatca
       61 tgatggtaca gcgtgattgc ttgattctca actttcccag acaaaaaggt ccaatagtag
      121 gtcagatggc tatgactctc tagaacccga taagaagttt catccgcatg aagtaagggc
      181 tgagtcaata gtctctctcg caagaggtta taaaggggct ttaggaata
//
